-- dump date 20140619_154720 -- class Genbank::CDS -- table cds -- table main -- field 1 id -- field 2 GI -- field 3 GeneID -- field 4 chrom_position -- field 5 chromosome -- field 6 codon_start -- field 7 contig -- field 8 description -- field 9 end_pos -- field 10 gene -- field 11 gene_id -- field 12 name -- field 13 organism -- field 14 product -- field 15 protein_id -- field 16 start_pos -- field 17 strand -- field 18 taxid -- field 19 type -- header -- id GI GeneID chrom_position chromosome codon_start contig description end_pos gene gene_id name organism product protein_id start_pos strand taxid type NP_214515.1 15607143 885041 1..1524 1 NC_000962.3 Rv0001, (MT0001, MTV029.01, P49993), len: 507 aa. dnaA, chromosomal replication initiator protein (see citations below), equivalent to other Mycobacterial chromosomal replication initiator proteins. Also highly similar to others except in N-terminus e.g. Q9ZH75|DNAA_STRCH chromosomal replication initiator protein from Streptomyces chrysomallus (624 aa). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS01008 DnaA protein signature. Belongs to the DnaA family. Note that the first base of this gene has been taken as base 1 of the Mycobacterium tuberculosis H37Rv genomic sequence.; Chromosomal replication initiator protein DnaA 1524 dnaA 885041 dnaA Mycobacterium tuberculosis H37Rv Chromosomal replication initiator protein DnaA NP_214515.1 1 D 83332 CDS NP_214516.1 15607144 887092 2052..3260 1 NC_000962.3 Rv0002, (MTV029.02, MTCY10H4.0), len: 402 aa. DnaN,DNA polymerase III (beta chain) (see citations below),equivalent to other Mycobacterial DNA polymerases III beta chain. Also highly similar to others e.g. P27903|DP3B_STRCO DNA polymerase III beta chain from Streptomyces coelicolor (376 aa). Overlaps and extends CDS in neighbouring cosmid MTCY10H4.01.; DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase) 3260 dnaN 887092 dnaN Mycobacterium tuberculosis H37Rv DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase) NP_214516.1 2052 D 83332 CDS NP_214517.1 15607145 887089 3280..4437 1 NC_000962.3 Rv0003, (MTCY10H4.01), len: 385 aa. RecF, DNA replication and repair protein (see citations below),equivalent to other mycobacterial DNA replication and repair proteins. Also highly similar to many others. Contains PS00017 ATP/GTP-binding site motif A (P-loop),PS00617 RecF protein signature 1, and PS00618 RecF protein signature 2. Belongs to the RecF family.; DNA replication and repair protein RecF (single-strand DNA binding protein) 4437 recF 887089 recF Mycobacterium tuberculosis H37Rv DNA replication and repair protein RecF (single-strand DNA binding protein) NP_214517.1 3280 D 83332 CDS NP_214518.1 15607146 887088 4434..4997 1 NC_000962.3 Rv0004, (MTCY10H4.02), len: 187 aa. Conserved hypothetical protein (see Salazar et al., 1996). Belongs to superfamily DUF721; this family contains several actinomycete proteins of unknown function.; hypothetical protein 4997 887088 Rv0004 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214518.1 4434 D 83332 CDS NP_214519.2 448824735 887081 5240..7267 1 NC_000962.3 Rv0005, (MTCY10H4.03), len: 675 aa. GyrB, DNA gyrase subunit B (see citations below). Contains PS00177 DNA topoisomerase II signature. Belongs to the type II topoisomerase family. Start changed since first submission (-39 aa).; DNA gyrase (subunit B) GyrB (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) 7267 gyrB 887081 gyrB Mycobacterium tuberculosis H37Rv DNA gyrase (subunit B) GyrB (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) NP_214519.2 5240 D 83332 CDS NP_214520.1 15607148 887105 7302..9818 1 NC_000962.3 Rv0006, (MTCY10H4.04), len: 838 aa. GyrA, DNA gyrase subunit A (see citations below). Contains PS00018 EF-hand calcium-binding domain.; DNA gyrase (subunit A) GyrA (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) 9818 gyrA 887105 gyrA Mycobacterium tuberculosis H37Rv DNA gyrase (subunit A) GyrA (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) NP_214520.1 7302 D 83332 CDS NP_214521.1 15607149 885982 9914..10828 1 NC_000962.3 Rv0007, (MTCY10H4.05), len: 304 aa. Possible conserved membrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Possible conserved membrane protein 10828 885982 Rv0007 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_214521.1 9914 D 83332 CDS NP_214522.1 15607150 887085 complement(11874..12311) 1 NC_000962.3 Rv0008c, (MTCY10H4.07c), len: 145 aa. Possible membrane protein.; Possible membrane protein 12311 887085 Rv0008c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_214522.1 11874 R 83332 CDS NP_214523.1 15607151 887087 12468..13016 1 NC_000962.3 Rv0009, (MTCY10H4.08), len: 182 aa. Probable ppiA (alternate gene name: cfp22), iron-regulated peptidyl-prolyl cis-trans isomerase A. Belongs to the cyclophilin-type PPIase family. Alternative start codon has been suggested.; Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A) 13016 ppiA 887087 ppiA Mycobacterium tuberculosis H37Rv Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A) NP_214523.1 12468 D 83332 CDS NP_214524.1 15607152 887082 complement(13133..13558) 1 NC_000962.3 Rv0010c, (MTCY10H4.10c), len: 141 aa. Probable conserved membrane protein. Belongs to superfamily DUF2581,conserved in the Actinomycetales. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved membrane protein 13558 887082 Rv0010c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214524.1 13133 R 83332 CDS NP_214525.1 15607153 887074 complement(13714..13995) 1 NC_000962.3 Rv0011c, (MTCY10H4.11c), len: 93 aa. Probable conserved transmembrane protein. Belongs to uncharacterized protein family UPF0233. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved transmembrane protein 13995 887074 Rv0011c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214525.1 13714 R 83332 CDS NP_214526.1 15607154 887083 14089..14877 1 NC_000962.3 Rv0012, (MTCY10H4.12), len: 262 aa. Probable conserved membrane protein. Belongs to superfamily DUF881. Contains probable N-terminal signal sequence.; Probable conserved membrane protein 14877 887083 Rv0012 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214526.1 14089 D 83332 CDS YP_177615.1 57116682 885955 14914..15612 1 NC_000962.3 Rv0013, (MTCY10H4.13), len: 232 aa. Possible trpG,anthranilate synthase component II (glutamine amidotransferase). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. Similarity to other type-1 glutamine amidotransferase domains. Note that previously known as pabA.; Possible anthranilate synthase component II TrpG (glutamine amidotransferase) 15612 trpG 885955 trpG Mycobacterium tuberculosis H37Rv Possible anthranilate synthase component II TrpG (glutamine amidotransferase) YP_177615.1 14914 D 83332 CDS NP_214528.1 15607156 887072 complement(15590..17470) 1 NC_000962.3 Rv0014c, (MTCY10H4.14c), len: 626 aa. PknB,transmembrane serine/threonine-protein kinase (see citations below). Contains PS00107 Protein kinases ATP-binding region signature, and PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on serine/threonine residues. PknB has been shown to be a substrate for PstP and its kinase activity is affected by PstP-mediated dephosphorylation. PknB and PstP (Rv0018c) may act as a functional pair in vivo to control mycobacterial cell growth.; Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B) 17470 pknB 887072 pknB Mycobacterium tuberculosis H37Rv Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B) NP_214528.1 15590 R 83332 CDS NP_214529.1 15607157 885953 complement(17467..18762) 1 NC_000962.3 Rv0015c, (MTCY10H4.15c), len: 431 aa. PknA,transmembrane serine/threonine-protein kinase,magnesium/manganese dependent (see citations below). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. It has been shown that sodium orthovanadate inhibits the activity of the enzyme in vitro.; Transmembrane serine/threonine-protein kinase A PknA (protein kinase A) (STPK A) 18762 pknA 885953 pknA Mycobacterium tuberculosis H37Rv Transmembrane serine/threonine-protein kinase A PknA (protein kinase A) (STPK A) NP_214529.1 17467 R 83332 CDS NP_214530.1 15607158 887078 complement(18759..20234) 1 NC_000962.3 Rv0016c, (MTCY10H4.16c), len: 491 aa. Probable pbpA,penicillin-binding protein, similar to many.; Probable penicillin-binding protein PbpA 20234 pbpA 887078 pbpA Mycobacterium tuberculosis H37Rv Probable penicillin-binding protein PbpA NP_214530.1 18759 R 83332 CDS NP_214531.1 15607159 887075 complement(20231..21640) 1 NC_000962.3 Rv0017c, (MTCY10H4.17c), len: 469 aa. Probable rodA (alternate gene name: ftsW), cell division protein,integral membrane protein. Belongs to the FTSW/RODA/SPOVE family.; Probable cell division protein RodA 21640 rodA 887075 rodA Mycobacterium tuberculosis H37Rv Probable cell division protein RodA NP_214531.1 20231 R 83332 CDS NP_214532.1 15607160 887070 complement(21637..23181) 1 NC_000962.3 Rv0018c, (MTCY10H4.18c), len: 514 aa. PstP,phosphoserine/threonine phosphatase. Experimental studies have shown that PstP specifically dephosporylates model phospho-Ser/Thr substrates and it is likely that PknB (Rv0014c) and PstP may act as a functional pair in vivo to control mycobacterial cell growth (See Boitel et al.,2003).; Phosphoserine/threonine phosphatase PstP 23181 pstP 887070 pstP Mycobacterium tuberculosis H37Rv Phosphoserine/threonine phosphatase PstP NP_214532.1 21637 R 83332 CDS NP_214533.1 15607161 887079 complement(23270..23737) 1 NC_000962.3 Rv0019c, (MTCY10H4.19c), len: 155 aa. FhaB,conserved protein with forkhead-associated domain (IPR000253), probably involved in signal transduction.; Conserved protein with FHA domain, FhaB 23737 fhaB 887079 fhaB Mycobacterium tuberculosis H37Rv Conserved protein with FHA domain, FhaB NP_214533.1 23270 R 83332 CDS NP_214534.1 15607162 887067 complement(23861..25444) 1 NC_000962.3 Rv0020c, (MTCY10H4.20c), len: 527 aa. FhaA, TB39.8,conserved protein with forkhead-associated domain (IPR000253) at C-terminus, may be involved in signal transduction. Alternative start codon in position 24979 has been suggested (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Conserved protein with FHA domain, FhaA 25444 fhaA 887067 fhaA Mycobacterium tuberculosis H37Rv Conserved protein with FHA domain, FhaA NP_214534.1 23861 R 83332 CDS NP_214535.1 15607163 887066 complement(25913..26881) 1 NC_000962.3 Rv0021c, (MTCY10H4.21c), len: 322 aa. Conserved hypothetical protein, similar to various proteins.; hypothetical protein 26881 887066 Rv0021c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214535.1 25913 R 83332 CDS NP_214536.1 15607164 887071 complement(27023..27442) 1 NC_000962.3 Rv0022c, (MTCY10H4.22c), len: 139 aa. Probable whiB5 (alternate gene name: whmG), WhiB-like regulatory protein (see citations below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa).; Probable transcriptional regulatory protein WhiB-like WhiB5 27442 whiB5 887071 whiB5 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein WhiB-like WhiB5 NP_214536.1 27023 R 83332 CDS NP_214537.1 15607165 887062 27595..28365 1 NC_000962.3 Rv0023, (MTCY10H4.23), len: 256 aa. Possible transcriptional regulator. Contains probable helix-turn helix motif from aa 19 to 40 (Score 1615, +4.69 SD).; Possible transcriptional regulatory protein 28365 887062 Rv0023 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214537.1 27595 D 83332 CDS NP_214538.1 15607166 887061 28362..29207 1 NC_000962.3 Rv0024, (MTCY10H4.24), len: 281 aa. Putative secreted protein, p60 homologue, similar to many. Similar to Mycobacterium tuberculosis proteins Rv1477, Rv1478,Rv1566c, Rv2190c. Could belong to the E. coli NLPC / listeria P60 family.; Putative secreted protein P60-related protein 29207 887061 Rv0024 Mycobacterium tuberculosis H37Rv Putative secreted protein P60-related protein NP_214538.1 28362 D 83332 CDS NP_214539.1 15607167 887060 29245..29607 1 NC_000962.3 Rv0025, (MTCY10H4.25), len: 120 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. Rv0739 (268 aa), FASTA score: (37.6% identity in 101 aa overlap), and Rv0026 FASTA score: (35.4% identity in 113 aa overlap); etc.; hypothetical protein 29607 887060 Rv0025 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214539.1 29245 D 83332 CDS NP_214540.1 15607168 887057 29722..31068 1 NC_000962.3 Rv0026, (MTCY10H4.26), len: 448 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis: Rv0025 FASTA score: (35.4% identity in 113 aa overlap) and Rv0739 (268 aa), FASTA score: (32.4% identity in 142 aa overlap).; hypothetical protein 31068 887057 Rv0026 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214540.1 29722 D 83332 CDS NP_214541.1 15607169 887054 31189..31506 1 NC_000962.3 Rv0027, (MTCY10H4.27), len: 105 aa. Conserved hypothetical unknown protein.; hypothetical protein 31506 887054 Rv0027 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214541.1 31189 D 83332 CDS NP_214542.1 15607170 885812 31514..31819 1 NC_000962.3 Rv0028, (MTCY10H4.28), len: 101 aa. Conserved hypothetical unknown protein.; hypothetical protein 31819 885812 Rv0028 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214542.1 31514 D 83332 CDS NP_214543.1 15607171 887053 32057..33154 1 NC_000962.3 Rv0029, (MTCY10H4.29), len: 365 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. C-terminal region of Rv2082; Rv3899c.; hypothetical protein 33154 887053 Rv0029 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214543.1 32057 D 83332 CDS NP_214544.1 15607172 887051 33224..33553 1 NC_000962.3 Rv0030, (MTCY10H4.30), len: 109 aa. Conserved hypothetical unknown protein.; hypothetical protein 33553 887051 Rv0030 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214544.1 33224 D 83332 CDS NP_214545.1 15607173 887049 33582..33794 1 NC_000962.3 Rv0031, (MTCY10H4.31), len: 70 aa. Possible remnant of a transposase, showing partial similarity to mycobacterial transposases in a short overlap, e.g. Rv2791c|MTV002_57 (459 aa), FASTA score: (72.2% identity in 36 aa overlap); Rv2885c, Rv2978c, Rv3827c, etc.; Possible remnant of a transposase 33794 887049 Rv0031 Mycobacterium tuberculosis H37Rv Possible remnant of a transposase NP_214545.1 33582 D 83332 CDS NP_214546.1 15607174 887050 34295..36610 1 NC_000962.3 Rv0032, (MTCY10H4.32), len: 771 aa. Probable bioF2,8-amino-7-oxononanoate synthase, with its C-terminal similar to others. Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases.; Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) 36610 bioF2 887050 bioF2 Mycobacterium tuberculosis H37Rv Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) NP_214546.1 34295 D 83332 CDS NP_214547.1 15607175 887052 36607..36870 1 NC_000962.3 Rv0033, (MTCY10H4.33), len: 87 aa. Probable acpA (alternate gene name: acpP), acyl carrier protein, similar to others. Also similar to proteins of Mycobacterium tuberculosis Rv1344 and Rv2244 (31.5% identity in 73 aa overlap).; Probable acyl carrier protein AcpA (ACP) 36870 acpA 887052 acpA Mycobacterium tuberculosis H37Rv Probable acyl carrier protein AcpA (ACP) NP_214547.1 36607 D 83332 CDS NP_214548.1 15607176 887046 36867..37262 1 NC_000962.3 Rv0034, (MTCY10H4.34), len: 131 aa. Conserved hypothetical protein, showing weak similarity to AE001980|AE001980_7 hypothetical protein from Deinococcus radiodurans (120 aa), FASTA scores: opt: 141, E(): 0.0028,(29.3% identity in 123 aa overlap).; hypothetical protein 37262 887046 Rv0034 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214548.1 36867 D 83332 CDS YP_177686.1 57116683 887048 37259..38947 1 NC_000962.3 Rv0035, (MTCY10H4.35), len: 562 aa. Probable fadD34,fatty-acid-CoA synthetase, similar to many.; Probable fatty-acid-CoA ligase FadD34 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 38947 fadD34 887048 fadD34 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD34 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) YP_177686.1 37259 D 83332 CDS NP_214550.1 15607178 887043 complement(39056..39829) 1 NC_000962.3 Rv0036c, (MTCY10H4.36c), len: 257 aa. Conserved protein, highly similar to CAB95889.1|AL359988 conserved hypothetical protein from Streptomyces (276 aa). Also some similarity to Rv3099c|MTCY164_10 (283 aa), FASTA scores: E(): 3.3e-05, (25.9% identity in 205 aa overlap).; hypothetical protein 39829 887043 Rv0036c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214550.1 39056 R 83332 CDS NP_214551.1 15607179 887042 complement(39877..41202) 1 NC_000962.3 Rv0037c, (MTCY10H4.37c), len: 441 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of macrolide.; Probable conserved integral membrane protein 41202 887042 Rv0037c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_214551.1 39877 R 83332 CDS NP_214552.1 15607180 887045 41304..41912 1 NC_000962.3 Rv0038, (MTCY10H4.38), len: 202 aa. Conserved protein, similar to many.; hypothetical protein 41912 887045 Rv0038 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214552.1 41304 D 83332 CDS NP_214553.1 15607181 887038 complement(42004..42351) 1 NC_000962.3 Rv0039c, (MTCY21D4.02c, MTCY10H4.39c), len: 115 aa. Possible conserved transmembrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved transmembrane protein 42351 887038 Rv0039c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_214553.1 42004 R 83332 CDS NP_214554.2 57116684 887037 complement(42433..43365) 1 NC_000962.3 Rv0040c, (MTCY21D4.03c), len: 310 aa. Mtc28,secreted proline rich 28 kDa antigen protein (has hydrophobic stretch at N-terminus) (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Secreted proline rich protein Mtc28 (proline rich 28 kDa antigen) 43365 mtc28 887037 mtc28 Mycobacterium tuberculosis H37Rv Secreted proline rich protein Mtc28 (proline rich 28 kDa antigen) NP_214554.2 42433 R 83332 CDS NP_214555.1 15607183 887040 43562..46471 1 NC_000962.3 Rv0041, (MTCY21D4.04), len: 969 aa. Probable leucyl-tRNA synthetase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family.; Probable leucyl-tRNA synthetase LeuS (leucine--tRNA ligase) (LEURS) 46471 leuS 887040 leuS Mycobacterium tuberculosis H37Rv Probable leucyl-tRNA synthetase LeuS (leucine--tRNA ligase) (LEURS) NP_214555.1 43562 D 83332 CDS NP_214556.1 15607184 887034 complement(46581..47207) 1 NC_000962.3 Rv0042c, (MTCY21D4.05c), len: 208 aa. Possible transcriptional regulatory protein, MarR-family. Some similarity to Mycobacterium tuberculosis proteins Rv2327,Rv0880, and Rv1404.; Possible transcriptional regulatory protein (probably MarR-family) 47207 887034 Rv0042c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably MarR-family) NP_214556.1 46581 R 83332 CDS NP_214557.1 15607185 887032 complement(47366..48100) 1 NC_000962.3 Rv0043c, (MTCY21D4.06c), len: 244 aa. Probable transcriptional regulator, GntR family, similar to others.; Probable transcriptional regulatory protein (probably GntR-family) 48100 887032 Rv0043c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably GntR-family) NP_214557.1 47366 R 83332 CDS NP_214558.1 15607186 887030 complement(48233..49027) 1 NC_000962.3 Rv0044c, (MTCY21D4.07c), len: 264 aa. Possible oxidoreductase, highly similar to AAD32732.1|MmcI|AF127374| F420-dependent H4MPT reductase from Streptomyces lavendulae (264 aa). Also similar to Mycobacterium tuberculosis proteins e.g. Rv1855c, Rv0953c, Rv0791c, Rv0132c, etc.; Possible oxidoreductase 49027 887030 Rv0044c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214558.1 48233 R 83332 CDS NP_214559.1 15607187 887029 complement(49043..49939) 1 NC_000962.3 Rv0045c, (MTCY21D4.08c), len: 298 aa. Possible hydrolase, showing similarity with others. Also similar to Mycobacterium tuberculosis proteins Rv3473c, Rv1123c,Rv1938, Rv3617, Rv3670, etc.; Possible hydrolase 49939 887029 Rv0045c Mycobacterium tuberculosis H37Rv Possible hydrolase NP_214559.1 49043 R 83332 CDS NP_214560.1 15607188 887028 complement(50021..51124) 1 NC_000962.3 Rv0046c, (MTCY21D4.09c), len: 367 aa. Ino1 (alternate gene name: tbINO), myo-inositol-1-phosphate synthase (see citations below).; myo-inositol-1-phosphate synthase Ino1 (inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (glucose 6-phosphate cyclase) (glucocycloaldolase) 51124 ino1 887028 ino1 Mycobacterium tuberculosis H37Rv myo-inositol-1-phosphate synthase Ino1 (inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (glucose 6-phosphate cyclase) (glucocycloaldolase) NP_214560.1 50021 R 83332 CDS NP_214561.1 15607189 887031 complement(51185..51727) 1 NC_000962.3 Rv0047c, (MTCY21D4.10c), len: 180 aa. Conserved protein, similar to many.; hypothetical protein 51727 887031 Rv0047c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214561.1 51185 R 83332 CDS NP_214562.1 15607190 887027 complement(51828..52697) 1 NC_000962.3 Rv0048c, MTCY21D4.11c, len: 289 aa. Possible membrane protein.; Possible membrane protein 52697 887027 Rv0048c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_214562.1 51828 R 83332 CDS NP_214563.1 15607191 887024 52831..53244 1 NC_000962.3 Rv0049, (MTCY21D4.12), len: 137 aa. Conserved hypothetical protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 53244 887024 Rv0049 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214563.1 52831 D 83332 CDS YP_177687.1 57116685 887065 53663..55699 1 NC_000962.3 Rv0050, (MTCY21D4.13), len: 678 aa. Probable ponA1,penicillin-binding protein (class A), bienzymatic protein with transglycosylase and transpeptidase activities (see Graham & Clark-Curtiss 1999), highly similar to many (see Billman-Jacobe et al., 1999). Belongs to the transglycosylase family in the N-terminal section, and to the transpeptidase family in the C-terminal section.; Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase) 55699 ponA1 887065 ponA1 Mycobacterium tuberculosis H37Rv Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase) YP_177687.1 53663 D 83332 CDS NP_214565.1 15607193 887018 55696..57378 1 NC_000962.3 Rv0051, (MTCY21D4.14), len:560 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein.; Probable conserved transmembrane protein 57378 887018 Rv0051 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214565.1 55696 D 83332 CDS NP_214566.3 448824736 887015 57410..57973 1 NC_000962.3 Rv0052, (MTCY21D4.15), len: 187 aa. Conserved protein, similar to others including Rv1930c from Mycobacterium tuberculosis (174 aa). May be a membrane protein.; hypothetical protein 57973 887015 Rv0052 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214566.3 57410 D 83332 CDS NP_214567.1 15607195 887020 58192..58482 1 NC_000962.3 Rv0053, (MTCY21D4.16), len: 96 aa. rpsF, 30S ribosomal protein S6, highly similar to many. Contains PS01048 Ribosomal protein S6 signature. Belongs to the S6P family of ribosomal proteins.; 30S ribosomal protein S6 RpsF 58482 rpsF 887020 rpsF Mycobacterium tuberculosis H37Rv 30S ribosomal protein S6 RpsF NP_214567.1 58192 D 83332 CDS NP_214568.1 15607196 887013 58586..59080 1 NC_000962.3 Rv0054, (MTCY21D4.17), len: 164 aa. ssb,single-strand binding protein (see Mizrahi & Andersen 1998), highly similar to others. Belongs to the SSB family.; Single-strand binding protein Ssb (helix-destabilizing protein) 59080 ssb 887013 ssb Mycobacterium tuberculosis H37Rv Single-strand binding protein Ssb (helix-destabilizing protein) NP_214568.1 58586 D 83332 CDS YP_177688.1 57116686 887022 59122..59376 1 NC_000962.3 Rv0055, (MTCY21D4.18), len: 84 aa. rpsR1, 30S ribosomal protein S18-1. Belongs to the S18P family of ribosomal proteins. Note that previously known as rpsR.; 30S ribosomal protein S18-1 RpsR1 59376 rpsR1 887022 rpsR1 Mycobacterium tuberculosis H37Rv 30S ribosomal protein S18-1 RpsR1 YP_177688.1 59122 D 83332 CDS NP_214570.1 15607198 887010 59409..59867 1 NC_000962.3 Rv0056, (MTCY21D4.19), len: 152 aa. rplI, 50S ribosomal protein L9. Contains PS00651 Ribosomal protein L9 signature. Belongs to the L9P family of ribosomal proteins.; 50S ribosomal protein L9 RplI 59867 rplI 887010 rplI Mycobacterium tuberculosis H37Rv 50S ribosomal protein L9 RplI NP_214570.1 59409 D 83332 CDS NP_214571.1 15607199 887008 59896..60417 1 NC_000962.3 Rv0057, (MTCY21D4.20), len: 173 aa. Hypothetical unknown protein.; Hypothetical protein 60417 887008 Rv0057 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214571.1 59896 D 83332 CDS NP_214572.1 15607200 887009 60396..63020 1 NC_000962.3 Rv0058, (MTV030.01, MTCY21D4.21), len: 874 aa. Probable dnaB, replicative DNA helicase. Contains an intein (position 61630..62838) similar to, and in the same position as, those in Sycnechocystis and Rhodothermus marinus (see citation below) and C-terminal extein (position 62839..63015) similar to many dnaB proteins. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the helicase family, DNAB subfamily. In the intein section; belongs to the homing endonuclease family.; Probable replicative DNA helicase DnaB 63020 dnaB 887009 dnaB Mycobacterium tuberculosis H37Rv Probable replicative DNA helicase DnaB NP_214572.1 60396 D 83332 CDS NP_214573.1 15607201 887006 63200..63892 1 NC_000962.3 Rv0059, (MTV030.02), len: 230 aa. Hypothetical unknown protein.; Hypothetical protein 63892 887006 Rv0059 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214573.1 63200 D 83332 CDS NP_214574.1 15607202 887004 63909..64967 1 NC_000962.3 Rv0060, (MTV030.03), len: 352 aa. Conserved hypothetical protein.; hypothetical protein 64967 887004 Rv0060 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214574.1 63909 D 83332 CDS YP_007408697.1 448814824 14515843 complement(65012..65350) 1 NC_000962.3 Rv0061c, len: 112 aa. Conserved hypothetical protein supported by RNA-seq data. Similar to MMAR_3839, 76% identity in 112 aa overlap. Replaces questionable ORF Rv0061 (MTV030.04).; Hypothetical protein 65350 14515843 Rv0061c Mycobacterium tuberculosis H37Rv Hypothetical protein YP_007408697.1 65012 R 83332 CDS YP_177689.1 57116687 887007 65552..66694 1 NC_000962.3 Rv0062, (MTV030.05), len: 380 aa. Possible celA1,cellulase, similar to many. Seems to belong to cellulase family B (family 6 of glycosyl hydrolases). Note that previously known as celA.; Possible cellulase CelA1 (endoglucanase) (endo-1,4-beta-glucanase) (FI-cmcase) (carboxymethyl cellulase) 66694 celA1 887007 celA1 Mycobacterium tuberculosis H37Rv Possible cellulase CelA1 (endoglucanase) (endo-1,4-beta-glucanase) (FI-cmcase) (carboxymethyl cellulase) YP_177689.1 65552 D 83332 CDS NP_214577.1 15607205 886999 66923..68362 1 NC_000962.3 Rv0063, (MTV030.06), len: 479 aa. Possible oxidoreductase, similar to many. Similar to Mycobacterium tuberculosis proteins e.g. Rv3107c, Rv1257c, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site.; Possible oxidoreductase 68362 886999 Rv0063 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214577.1 66923 D 83332 CDS NP_214578.1 15607206 886996 68620..71559 1 NC_000962.3 Rv0064, (MTV030.07), len: 979 aa. Probable conserved transmembrane protein, similar to many. Contains probable coiled-coil domain from aa 948 to 976.; Probable conserved transmembrane protein 71559 886996 Rv0064 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214578.1 68620 D 83332 CDS YP_007408701.1 448814828 14515844 71589..71828 1 NC_000962.3 Rv0064A, len: 79 aa. Possible vapB1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0065 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv0300 (73 aa),Rv1721c (75 aa); Possible antitoxin VapB1 71828 vapB1 14515844 vapB1 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB1 YP_007408701.1 71589 D 83332 CDS NP_214579.1 15607207 886993 71821..72222 1 NC_000962.3 Rv0065, (MTV030.08), len: 133 aa. Possible vapC1,toxin, part of toxin-antitoxin (TA) operon with Rv0064A,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in Mycobacterium tuberculosis: Rv0960 (127 aa), Rv1720c (129 aa), and Rv0549c (137 aa).; Possible toxin VapC1 72222 vapC1 886993 vapC1 Mycobacterium tuberculosis H37Rv Possible toxin VapC1 NP_214579.1 71821 D 83332 CDS NP_214580.1 15607208 887016 complement(72274..74511) 1 NC_000962.3 Rv0066c, (MTV030.09c), len: 745 aa. Probable icd2,isocitrate dehydrogenase NADP-dependent. Belongs to the monomeric-type family of IDH. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2.; Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) 74511 icd2 887016 icd2 Mycobacterium tuberculosis H37Rv Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) NP_214580.1 72274 R 83332 CDS NP_214581.1 15607209 886991 complement(74629..75198) 1 NC_000962.3 Rv0067c, (MTV030.10c), len: 189 aa. Possible transcriptional regulator, highly similar to many. Contains probable helix-turn-helix motif from aa 34 to 55 (Score 1523, +4.37 SD).; Possible transcriptional regulatory protein (possibly TetR-family) 75198 886991 Rv0067c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (possibly TetR-family) NP_214581.1 74629 R 83332 CDS NP_214582.1 15607210 886989 75301..76212 1 NC_000962.3 Rv0068, (MTV030.11), len: 303 aa. Probable oxidoreductase, similar to many.; Probable oxidoreductase 76212 886989 Rv0068 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_214582.1 75301 D 83332 CDS NP_214583.1 15607211 886986 complement(76237..77622) 1 NC_000962.3 Rv0069c, (MTV030.12c), len: 461 aa. Probable sdaA,L-serine dehydratase. Belongs to the iron-sulfur dependent L-serine dehydratase family. Cofactor: iron-sulfur (4FE-4S) (probable).; Probable L-serine dehydratase SdaA (L-serine deaminase) (SDH) (L-SD) 77622 sdaA 886986 sdaA Mycobacterium tuberculosis H37Rv Probable L-serine dehydratase SdaA (L-serine deaminase) (SDH) (L-SD) NP_214583.1 76237 R 83332 CDS NP_214584.1 15607212 886983 complement(77619..78896) 1 NC_000962.3 Rv0070c, (MTV030.13c), len: 425 aa. glyA2, serine hydroxymethyltransferase. Contains PS00096 Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. Belongs to the ShmT family. Cofactor: pyridoxal phosphate.; Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2) 78896 glyA2 886983 glyA2 Mycobacterium tuberculosis H37Rv Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2) NP_214584.1 77619 R 83332 CDS NP_214585.1 15607213 886988 79486..80193 1 NC_000962.3 Rv0071, (MTV030.14), len: 235 aa. Possible maturase,similar to many proteins of the group II intron maturase family. Contains 5 VDP repeats at N-terminus, these are also found in two Streptococcus plasmid hypothetical proteins Q52246|X17092 and Q54942|X66468.; Possible maturase 80193 886988 Rv0071 Mycobacterium tuberculosis H37Rv Possible maturase NP_214585.1 79486 D 83332 CDS NP_214586.1 15607214 886984 80624..81673 1 NC_000962.3 Rv0072, (MTV030.16), len: 349 aa. Probable glutamine-transport transmembrane protein ABC-transporter (see citation below). Note that supposed act with near ORF Rv0073|MTV030.17 ATP-binding protein ABC-transporter.; Probable glutamine-transport transmembrane protein ABC transporter 81673 886984 Rv0072 Mycobacterium tuberculosis H37Rv Probable glutamine-transport transmembrane protein ABC transporter NP_214586.1 80624 D 83332 CDS NP_214587.1 15607215 886977 81676..82668 1 NC_000962.3 Rv0073, (MTV030.17), len: 330 aa. Probable glutamine-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins. Contains PS00017 ATP/GTP-binding site motif A (P-loop),PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters). Note that supposed act with near ORF Rv0072|MTV030.16 transmembrane ABC-transporter.; Probable glutamine-transport ATP-binding protein ABC transporter 82668 886977 Rv0073 Mycobacterium tuberculosis H37Rv Probable glutamine-transport ATP-binding protein ABC transporter NP_214587.1 81676 D 83332 CDS NP_214588.1 15607216 886976 82748..83983 1 NC_000962.3 Rv0074, (MTV030.18), len: 411 aa. Conserved protein,similar to many.; hypothetical protein 83983 886976 Rv0074 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214588.1 82748 D 83332 CDS NP_214589.1 15607217 886982 83996..85168 1 NC_000962.3 Rv0075, (MTV030.19), len: 390 aa. Probable aminotransferase, similar to many class-II pyridoxal-phosphate-dependent aminotransferases (MALY/PATB subfamily). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv2294, Rv0858c, etc.; Probable aminotransferase 85168 886982 Rv0075 Mycobacterium tuberculosis H37Rv Probable aminotransferase NP_214589.1 83996 D 83332 CDS NP_214590.1 15607218 886992 complement(85183..85572) 1 NC_000962.3 Rv0076c, (MTV030.20c), len: 129 aa. Probable membrane protein, with membrane-spanning domain at C-terminus.; Probable membrane protein 85572 886992 Rv0076c Mycobacterium tuberculosis H37Rv Probable membrane protein NP_214590.1 85183 R 83332 CDS NP_214591.1 15607219 886969 complement(85636..86466) 1 NC_000962.3 Rv0077c, (MTV030.21c), len: 276 aa. Possible oxidoreductase, weakly similar to others from Streptomyces. Also similar to MTCY05A6_35 and MTCY1A11_10 from Mycobacterium tuberculosis. And shows some similarity in part with AAL17935.1|AY054120 putative epoxide hydrolase from Mycobacterium smegmatis (203 aa).; Probable oxidoreductase 86466 886969 Rv0077c Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_214591.1 85636 R 83332 CDS NP_214592.1 15607220 886990 86528..87133 1 NC_000962.3 Rv0078, (MTV030.22), len: 201 aa. Probable transcriptional regulator. Contains probable helix-turn-helix motif from aa 35 to 56 (Score 1348, +3.78 SD).; Probable transcriptional regulatory protein 87133 886990 Rv0078 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_214592.1 86528 D 83332 CDS YP_177616.1 57116688 3205053 complement(87208..87801) 1 NC_000962.3 Rv0078A, len: 197 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 87801 3205053 Rv0078A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177616.1 87208 R 83332 CDS YP_007408717.1 448814844 14515845 complement(87798..88004) 1 NC_000962.3 Rv0078B, len: 68 aa. Conserved protein.; hypothetical protein 88004 14515845 Rv0078B Mycobacterium tuberculosis H37Rv hypothetical protein YP_007408717.1 87798 R 83332 CDS NP_214593.1 15607221 886995 88204..89025 1 NC_000962.3 Rv0079, (MTV030.23), len: 273 aa. Unknown protein. Predicted possible vaccine candidate (See Zvi et al.,2008).; hypothetical protein 89025 886995 Rv0079 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214593.1 88204 D 83332 CDS NP_214594.1 15607222 886966 89022..89480 1 NC_000962.3 Rv0080, (MTV030.24), len: 152 aa. Conserved hypothetical protein. Belongs to pyridoxine 5'-phosphate (PNP) oxidase-like (PNPOx-like) superfamily.; hypothetical protein 89480 886966 Rv0080 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214594.1 89022 D 83332 CDS NP_214595.1 15607223 887012 89575..89919 1 NC_000962.3 Rv0081, (MTV030.25), len: 114 aa. Probable transcriptional regulator, highly similar to others.; Probable transcriptional regulatory protein 89919 887012 Rv0081 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_214595.1 89575 D 83332 CDS NP_214596.2 448824737 886968 89924..90403 1 NC_000962.3 Rv0082, (MTV030.26), len: 159 aa. Probable oxidoreductase, highly similar or similar to other various oxidoreductases. Nucleotide position 90144 in the genome sequence has been corrected, A:G resulting in Q74R.; Probable oxidoreductase 90403 886968 Rv0082 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_214596.2 89924 D 83332 CDS NP_214597.1 15607225 886965 90400..92322 1 NC_000962.3 Rv0083, (MTV030.27, MTCY251.01), len: 640 aa. Probable oxidoreductase, showing some similarity to other various oxidoreductases. Nucleotide position 91071 in the genome sequence has been corrected, T:C resulting in I224I.; Probable oxidoreductase 92322 886965 Rv0083 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_214597.1 90400 D 83332 CDS NP_214598.1 15607226 886959 92328..93278 1 NC_000962.3 Rv0084, (MTCY251.02), len: 316 aa. Possible hycD (alternate gene name: hevD), formate hydrogenlyase,integral membrane protein, similar to others. Belongs to the complex I subunit 1 family.; Possible formate hydrogenlyase HycD (FHL) 93278 hycD 886959 hycD Mycobacterium tuberculosis H37Rv Possible formate hydrogenlyase HycD (FHL) NP_214598.1 92328 D 83332 CDS NP_214599.1 15607227 886973 93289..93951 1 NC_000962.3 Rv0085, (MTCY251.03), len: 220 aa. Possible hycP,hydrogenase, integral membrane protein. Belongs to NADH-ubiquinone/plastoquinone oxidoreductase chain 4L superfamily.; Possible hydrogenase HycP 93951 hycP 886973 hycP Mycobacterium tuberculosis H37Rv Possible hydrogenase HycP NP_214599.1 93289 D 83332 CDS NP_214600.1 15607228 886963 93951..95417 1 NC_000962.3 Rv0086, (MTCY251.04), len: 488 aa. Possible hycQ,hydrogenase, integral membrane protein. Belongs to the NADH-Ubiquinone/plastoquinone (complex I) superfamily.; Possible hydrogenase HycQ 95417 hycQ 886963 hycQ Mycobacterium tuberculosis H37Rv Possible hydrogenase HycQ NP_214600.1 93951 D 83332 CDS NP_214601.1 15607229 886956 95414..96892 1 NC_000962.3 Rv0087, (MTCY251.05), len: 492 aa. Possible hycE (alternate gene name: hevE), formate hydrogenlyase, similar to others. Belongs to the complex I 49 kDa subunit family.; Possible formate hydrogenase HycE (FHL) 96892 hycE 886956 hycE Mycobacterium tuberculosis H37Rv Possible formate hydrogenase HycE (FHL) NP_214601.1 95414 D 83332 CDS NP_214602.1 15607230 886954 96927..97601 1 NC_000962.3 Rv0088, (MTCY251.06), len: 224 aa. Possible polyketide cyclase/dehydrase. Belongs to the SRPBCC ligand-binding domain superfamily. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible polyketide cyclase/dehydrase 97601 886954 Rv0088 Mycobacterium tuberculosis H37Rv Possible polyketide cyclase/dehydrase NP_214602.1 96927 D 83332 CDS NP_214603.1 15607231 886949 97758..98351 1 NC_000962.3 Rv0089, (MTCY251.07), len: 197 aa. Possible methyltransferase, showing some weak similarity to others. Also some similarity with many biotin biosynthesis proteins. Belongs to the methyltransferase superfamily.; Possible methyltransferase/methylase 98351 886949 Rv0089 Mycobacterium tuberculosis H37Rv Possible methyltransferase/methylase NP_214603.1 97758 D 83332 CDS NP_214604.1 15607232 886961 98480..99250 1 NC_000962.3 Rv0090, (MTCY251.08), len: 256 aa. Possible membrane protein. Contains IPR014511 Protein of unknown function DUF2068, transmembrane, subgroup.; Possible membrane protein 99250 886961 Rv0090 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_214604.1 98480 D 83332 CDS NP_214605.1 15607233 886953 99684..100451 1 NC_000962.3 Rv0091, (MTCY251.10), len: 255 aa. Probable mtn (alternate gene name: pfs),methylthioadenosine/S-Adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase), including 5'-methylthioadenosine nucleosidase and S-adenosylhomocysteine nucleosidase,similar to others. Belongs to the MTN family.; Probable bifunctional MTA/SAH nucleosidase Mtn: 5'-methylthioadenosine nucleosidase (methylthioadenosine methylthioribohydrolase) + S-adenosylhomocysteine nucleosidase (S-adenosyl-L-homocysteine homocysteinylribohydrolase) 100451 mtn 886953 mtn Mycobacterium tuberculosis H37Rv Probable bifunctional MTA/SAH nucleosidase Mtn: 5'-methylthioadenosine nucleosidase (methylthioadenosine methylthioribohydrolase) + S-adenosylhomocysteine nucleosidase (S-adenosyl-L-homocysteine homocysteinylribohydrolase) NP_214605.1 99684 D 83332 CDS NP_214606.1 15607234 886946 100583..102868 1 NC_000962.3 Rv0092, (MTCY251.11), len: 761 aa. CtpA,cation-transporting P-type ATPase a (transmembrane protein), highly similar to many. Contains PS01047 Heavy-metal-associated domain, and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB.; Cation transporter P-type ATPase a CtpA 102868 ctpA 886946 ctpA Mycobacterium tuberculosis H37Rv Cation transporter P-type ATPase a CtpA NP_214606.1 100583 D 83332 CDS NP_214607.1 15607235 886945 complement(102815..103663) 1 NC_000962.3 Rv0093c, (MTCY251.12c), len: 282 aa. Probable conserved membrane protein, equivalent only to CAC30943.1|AL583924 probable integral membrane protein from Mycobacterium leprae (237 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved membrane protein 103663 886945 Rv0093c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214607.1 102815 R 83332 CDS NP_214608.1 15607236 886943 complement(103710..104663) 1 NC_000962.3 Rv0094c, (MTCY251.13c), len: 317 aa. Member of 13E12 repeat family, showing some similarity to U15187|MLU15187_7 from Mycobacterium leprae (94 aa), FASTA score: (49.4% identity in 79 aa overlap).; hypothetical protein 104663 886943 Rv0094c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214608.1 103710 R 83332 CDS NP_214609.1 15607237 886940 complement(104805..105215) 1 NC_000962.3 Rv0095c, (MTCY251.14c), len: 136 aa. Member of 13E12 repeat, also partially similar to AF0418|AF041819_8 from Mycobacterium bovis BCG (222 aa), FASTA score: (89.6% identity in 96 aa overlap).; hypothetical protein 105215 886940 Rv0095c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214609.1 104805 R 83332 CDS YP_177690.1 57116689 886938 105324..106715 1 NC_000962.3 Rv0096, (MTCY251.15), len: 463 aa. PPE1, Member of the Mycobacterium tuberculosis PPE family, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; PPE family protein PPE1 106715 PPE1 886938 PPE1 Mycobacterium tuberculosis H37Rv PPE family protein PPE1 YP_177690.1 105324 D 83332 CDS NP_214611.1 15607239 886942 106734..107603 1 NC_000962.3 Rv0097, (MTCY251.16), len: 289 aa. Possible oxidoreductase, equivalent to NP_302343.1|NC_002677 putative oxidoreductase from Mycobacterium leprae (289 aa). Also highly similar to BAB69377.1|AB070955 putative oxidoreductase from Streptomyces avermitilis (296 aa). Contains PS00077 Cytochrome c oxidase subunit I, copper B binding region signature.; Possible oxidoreductase 107603 886942 Rv0097 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214611.1 106734 D 83332 CDS NP_214612.1 15607240 886935 107600..108151 1 NC_000962.3 Rv0098, (MTCY251.17), len: 183 aa. FcoT, long-chain fatty acyl CoA thioesterase type III (See Wang et al.,2007), equivalent to CAC30948.1|AL583924 from Mycobacterium leprae (183 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable fatty acyl CoA thioesterase type III FcoT 108151 fcoT 886935 fcoT Mycobacterium tuberculosis H37Rv Probable fatty acyl CoA thioesterase type III FcoT NP_214612.1 107600 D 83332 CDS NP_214613.1 15607241 886933 108156..109778 1 NC_000962.3 Rv0099, (MTCY251.18), len: 540 aa. Possible fadD10,fatty-acid-CoA synthetase, similar to many. Contains PS00455 putative AMP-binding domain signature. Contains IPR000873 AMP-dependent synthetase/ligase domain. Belongs to the ATP-dependent AMP-binding enzyme family.; Possible fatty-acid-CoA ligase FadD10 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 109778 fadD10 886933 fadD10 Mycobacterium tuberculosis H37Rv Possible fatty-acid-CoA ligase FadD10 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_214613.1 108156 D 83332 CDS NP_214614.1 15607242 886931 109783..110019 1 NC_000962.3 Rv0100, (MTCY251.19), len: 78 aa. Conserved hypothetical protein, equivalent only to CAC30950.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (78 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 110019 886931 Rv0100 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214614.1 109783 D 83332 CDS NP_214615.1 15607243 886951 110001..117539 1 NC_000962.3 Rv0101, (MTCY251.20), len: 2512 aa. Probable nrp,peptide synthetase, similar to others e.g. AAD44234.1|AF143772_40|PstB peptide synthetase from Mycobacterium avium (2552 aa); 7476034|S77657 cyclic peptide synthetase from Mycobacterium leprae (1401 aa),FASTA scores: opt: 4268, E(): 0, (65.7% identity in 1091 aa overlap); part of CAB55600.1|AJ238027 peptide synthetase from Mycobacterium smegmatis (5990). Also similar to e.g. AAD56240.1|AF184977_1|AF184977 DhbF protein from Bacillus subtilis (2378 aa); SRF1_BACSU|P27206 surfactin synthetase subunit 1 (3587 aa), FASTA scores: opt: 1708, E(): 0,(30.6% identity in 1633 aa overlap): etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), 2 x PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. Thought to be not involved in mycobactin biosynthesis (see citation below).; Probable peptide synthetase Nrp (peptide synthase) 117539 nrp 886951 nrp Mycobacterium tuberculosis H37Rv Probable peptide synthetase Nrp (peptide synthase) NP_214615.1 110001 D 83332 CDS NP_214616.1 15607244 886926 117714..119699 1 NC_000962.3 Rv0102, (MTCY251.21), len: 661 aa. Probable conserved integral membrane protein, highly similar to P53525|Y102_MYCLE|ML1998|NP_302349.1|NC_002677 possible membrane protein from Mycobacterium leprae (659 aa), FASTA scores: opt: 3107, E(): 0, (70.2% identity in 662 aa overlap). Also similar to others e.g. CAC01497.1|AL391017 putative integral membrane protein from Streptomyces coelicolor (316 aa); etc. Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide.; Probable conserved integral membrane protein 119699 886926 Rv0102 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_214616.1 117714 D 83332 CDS NP_214617.1 15607245 886928 complement(119915..122173) 1 NC_000962.3 Rv0103c, (MTCY251.22c), len: 752 aa. Probable ctpB,cation-transporting P-type ATPase B (transmembrane protein), equivalent to CTPB_MYCLE|P46840 cation-transporting P-type ATPase B from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615, E(): 0, (76.5% identity in 752 aa overlap). Also highly similar to others e.g. CAB96031.1|AL360055 putative metal transporter ATPase from Streptomyces coelicolor (753 aa); NP_241423.1|NC_002570 copper-transporting ATPase from Bacillus halodurans (806 aa); etc. Also highly similar to Z46257|MLACEA_7 aceA gene for isocitrate L from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615,E():0, (76.5% identity in 752 aa overlap). And similar to MTCY251.11 from Mycobacterium tuberculosis, FASTA score: (68.3% identity in 742 aa overlap). Contains PS01047 Heavy-metal-associated domain, PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB.; Probable cation-transporter P-type ATPase B CtpB 122173 ctpB 886928 ctpB Mycobacterium tuberculosis H37Rv Probable cation-transporter P-type ATPase B CtpB NP_214617.1 119915 R 83332 CDS NP_214618.1 15607246 886923 122317..123831 1 NC_000962.3 Rv0104, (MTCY251.23), len: 504 aa. Conserved hypothetical protein, showing weak similarity with other cAMP-dependent protein kinases e.g. AAC37564.1|M65066 cAMP-dependent protein kinase RI-beta regulatory subunit from Homo sapiens (380 aa); etc.; hypothetical protein 123831 886923 Rv0104 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214618.1 122317 D 83332 CDS YP_177691.1 57116690 886920 complement(123980..124264) 1 NC_000962.3 Rv0105c, (MTCY251.24c), len: 94 aa. rpmB1, 50S ribosomal protein L28-1, highly similar to others e.g. Q9X8K8|R28B_STRCO 50S ribosomal protein L28-2 from Streptomyces coelicolor (78 aa); RL28_ECOLI|P02428 50s ribosomal protein l28 from Escherichia coli (77 aa), FASTA scores: opt: 167, E(): 6.2e-06, (40.7% identity in 59 aa overlap); etc. Also similar to MTCY63A_2 from Mycobacterium tuberculosis. Belongs to the L28P family of ribosomal proteins.; 50S ribosomal protein L28-1 RpmB1 124264 rpmB1 886920 rpmB1 Mycobacterium tuberculosis H37Rv 50S ribosomal protein L28-1 RpmB1 YP_177691.1 123980 R 83332 CDS NP_214620.1 15607248 886919 124374..125570 1 NC_000962.3 Rv0106, (MTCY251.25), len: 398 aa. Conserved hypothetical protein, similar to others e.g. AL049841|SCE9_33 from Streptomyces coelicolor (370 aa),FASTA scores: opt: 282, E(): 2.5e-11, (32.0% identity in 381 aa overlap); etc. Some similarity to P94400 homologue to nitrile hydratase region from Bacillus subtilis (397 aa), FASTA scores: opt: 226, E(): 5.4e-08, (26.4% identity in 405 aa overlap). Also similar to COBW_PSEDE|P29937 FASTA score: (25.3% identity in 186 aa overlap); and P47K_PSECL|P31521 47 kDa protein (p47k) (419 aa), FASTA score: (25.9% identity in 401 aa overlap).; hypothetical protein 125570 886919 Rv0106 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214620.1 124374 D 83332 CDS NP_214621.1 15607249 886915 complement(125643..130541) 1 NC_000962.3 Rv0107c, (MTCY251.26c, MTV031.01c), len: 1632 aa. Probable ctpI, cation-transporting ATPase I P-type, highly similar to NP_302704.1|NC_002677 probable cation transport ATPase from Mycobacterium leprae (1609 aa); and similar to others e.g. CAB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); ATA1_SYNY|P37367 cation-transporting ATPase pma1 from Synechocystis sp. (915 aa), FASTA scores: opt: 603, E(): 6.6e-29, (32.4% identity in 710 aa overlap); etc. Also similar to MTCY39.21c and MTCY22G10.22c from Mycobacterium tuberculosis, FASTA score: (34.4% identity in 796 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases).; Probable cation-transporter ATPase I CtpI 130541 ctpI 886915 ctpI Mycobacterium tuberculosis H37Rv Probable cation-transporter ATPase I CtpI NP_214621.1 125643 R 83332 CDS NP_214622.1 15607250 886918 complement(130895..131104) 1 NC_000962.3 Rv0108c, (MTV031.02c), len: 69 aa. Hypothetical unknown protein.; Hypothetical protein 131104 886918 Rv0108c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214622.1 130895 R 83332 CDS YP_177692.1 57116691 886912 131382..132872 1 NC_000962.3 Rv0109, (MTV031.03c), len: 496 aa. PE_PGRS1, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Q50615|Y0DP_MYCTU hypothetical glycine-rich 40.8 kDa protein from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1772, E(): 0,(57.3% identity in 513 aa overlap); etc.; PE-PGRS family protein PE_PGRS1 132872 PE_PGRS1 886912 PE_PGRS1 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS1 YP_177692.1 131382 D 83332 CDS NP_214624.1 15607252 886917 133020..133769 1 NC_000962.3 Rv0110, (MTV031.04), len: 249 aa. Probable conserved integral membrane protein, similar to many e.g. AL079308|SCH69_25 from Streptomyces coelicolor (297 aa),FASTA scores: opt: 552, E(): 6.1e-29, (45.4% identity in 251 aa overlap); P54493|YQGP_BACSU hypothetical 56.4 KD protein from Bacillus subtilis (507 aa), FASTA scores: opt: 320, E(): 4e-15, (32.4% identity in 210 aa overlap); etc.; Probable conserved integral membrane protein 133769 886917 Rv0110 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_214624.1 133020 D 83332 CDS NP_214625.1 15607253 886909 133950..136007 1 NC_000962.3 Rv0111, (MTV031.05), len: 685 aa. Possible transmembrane acyltransferase, equivalent to AA22904.1|AL035300 putative acyltransferase from Mycobacterium leprae (696 aa). Also similar to others e.g. C69975 acyltransferase homolog yrhL from Bacillus subtilis (634 aa), FASTA scores: opt: 520, E(): 4e-22, (36.4% identity in 382 aa overlap). Very similar to Mycobacterium tuberculosis proteins Rv0228, Rv1254, Rv1565c, etc.; Possible transmembrane acyltransferase 136007 886909 Rv0111 Mycobacterium tuberculosis H37Rv Possible transmembrane acyltransferase NP_214625.1 133950 D 83332 CDS NP_214626.1 15607254 886907 136289..137245 1 NC_000962.3 Rv0112, (MTV031.06), len: 318 aa. Possible gca,GDP-mannose 4,6-dehydratase, similar to others e g. U18320|PAU18320_1 GDP-D-mann from Pseudomonas aeruginosa (323 aa), FASTA scores: opt: 415, E(): 4.4e-21, (27.0% identity in 318 aa overlap). Similar to Rv3634c, Rv3784,etc from Mycobacterium tuberculosis. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Seems to belong to the GDP-mannose 4,6-dehydratase family. Cofactor: NAD(+). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible GDP-mannose 4,6-dehydratase Gca (GDP-D-mannose dehydratase) 137245 gca 886907 gca Mycobacterium tuberculosis H37Rv Possible GDP-mannose 4,6-dehydratase Gca (GDP-D-mannose dehydratase) NP_214626.1 136289 D 83332 CDS NP_214627.1 15607255 886905 137319..137909 1 NC_000962.3 Rv0113, (MTV031.07), len: 196 aa. Probable gmhA (alternate gene name: lpcA), sedoheptulose-7-phosphate isomerase (see citation below), similar to many e.g. AE0005|HPAE000596_11 from Helicobacter pylori (192 aa),FASTA scores: opt: 451, E(): 1.9e-24, (45.1% identity in 162 aa overlap). Belongs to the sis family, LPCA subfamily. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable sedoheptulose-7-phosphate isomerase GmhA (phosphoheptose isomerase) 137909 gmhA 886905 gmhA Mycobacterium tuberculosis H37Rv Probable sedoheptulose-7-phosphate isomerase GmhA (phosphoheptose isomerase) NP_214627.1 137319 D 83332 CDS NP_214628.1 15607256 886903 137941..138513 1 NC_000962.3 Rv0114, (MTV031.08), len: 190 aa. Possible gmhB,D-alpha,beta-D-heptose-1,7-biphosphate phosphatase (see citation below), similar to several hypothetical proteins and phosphatases e.g. HIS7_ECOLI|P06987 imidazoleglycerol-phosphate dehydratase (355 aa), FASTA scores: opt: 250, E(): 3.6e-11, (34.0 % identity in 141 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible D-alpha,beta-D-heptose-1,7-biphosphate phosphatase GmhB (D-glycero-D-manno-heptose 7-phosphate kinase) 138513 gmhB 886903 gmhB Mycobacterium tuberculosis H37Rv Possible D-alpha,beta-D-heptose-1,7-biphosphate phosphatase GmhB (D-glycero-D-manno-heptose 7-phosphate kinase) NP_214628.1 137941 D 83332 CDS NP_214629.1 15607257 886902 138513..139673 1 NC_000962.3 Rv0115, (MTV031.09), len: 386 aa. Possible hddA,D-alpha-D-heptose-7-phosphate kinase (see citation below),similar to several hypothetical proteins and sugar kinases e.g. AAK27850.1|AF324836_3 D-glycero-D-manno-heptose 7-phosphate kinase from Aneurinibacillus thermoaerophilus (341 aa); AAK80995.1|AE007802_11 Sugar kinase from Clostridium acetobutylicum (364 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible D-alpha-D-heptose-7-phosphate kinase HddA 139673 hddA 886902 hddA Mycobacterium tuberculosis H37Rv Possible D-alpha-D-heptose-7-phosphate kinase HddA NP_214629.1 138513 D 83332 CDS NP_214630.1 15607258 886900 complement(140267..141022) 1 NC_000962.3 Rv0116c, (MTV031.10c), len: 251 aa. Probable ldtA,L,D-transpeptidase, showing similarity to several hypothetical mycobacterial proteins e.g. Rv1433 from Mycobacterium tuberculosis (271 aa); and Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa); to the C-terminal regions of others like Rv0192 from Mycobacterium tuberculosis (366 aa), FASTA scores: opt: 451, E(): 1.7e-21, (46.7% identity in 270 aa overlap); and Rv0192|Z97050|MTCI28_32 from Mycobacterium tuberculosis cosmid (366 aa), FASTA scores: opt: 699, E(): 0, (45.7% identity in 221 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable L,D-transpeptidase LdtA 141022 ldtA 886900 ldtA Mycobacterium tuberculosis H37Rv Probable L,D-transpeptidase LdtA NP_214630.1 140267 R 83332 CDS NP_214631.1 15607259 886914 141200..142144 1 NC_000962.3 Rv0117, (MTV031.11), len: 314 aa. OxyS, oxidative stress response protein regulatory protein, LysR family (see citation below). Similar to many transcription regulators and OxyR, the oxidative stress response protein of many bacteria. Contains LysR family signature at N-terminus. Also contains helix-turn-helix motif at aa 16-37 (Score 1543, +4.44 SD). Belongs to the LysR family of transcriptional regulators. OXYR is required for the induction of a regulon of hydrogen peroxide inducible genes such as catalase, glutathione-reductase, etc.; Oxidative stress response regulatory protein OxyS 142144 oxyS 886914 oxyS Mycobacterium tuberculosis H37Rv Oxidative stress response regulatory protein OxyS NP_214631.1 141200 D 83332 CDS NP_214632.1 15607260 886898 complement(142128..143876) 1 NC_000962.3 Rv0118c, (MTV031.12c), Len: 582 aa. Probable oxcA,oxalyl-CoA decarboxylase, highly similar to many e.g. P78093|OXC_ECOLI|7449483|B65011|YFDU|B2373|Z3637|ECS325 probable oxalyl-CoA decarboxylase from Escherichia coli (564 aa); M77128|OXAOXA_1 oxalyl-CoA decarboxylase from Oxalobacter formigenes (568 aa), FASTA scores: opt: 2124,E():0, (55.6% identity in 568 aa overlap). Also similar to mycobacterial IlvB proteins e.g. MLCB1788.46c unknown TPP-requiring enzyme from Mycobacterium leprae (548 aa); and AL0086|MLCB1788_19 from Mycobacterium leprae (548 aa),FASTA scores: opt: 831, E(): 0, (33.9% identity in 567 aa overlap).; Probable oxalyl-CoA decarboxylase OxcA 143876 oxcA 886898 oxcA Mycobacterium tuberculosis H37Rv Probable oxalyl-CoA decarboxylase OxcA NP_214632.1 142128 R 83332 CDS NP_214633.1 15607261 886896 144049..145626 1 NC_000962.3 Rv0119, (MTV031.13-MTCI418B.01), len: 525 aa. Probable fadD7, fatty-acid-CoA synthetase, similar to 4-coumarate:CoA ligase of many organisms e.g. U39405|PTU39405_1 4-coumarate:CoA ligase from Pinus taedaxylem (537 aa), FASTA scores: opt: 483, E(): 8.3e-22,(28.2% identity in 440 aa overlap). Contains PS00455 Putative AMP-binding domain signature.; Probable fatty-acid-CoA ligase FadD7 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 145626 fadD7 886896 fadD7 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD7 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_214633.1 144049 D 83332 CDS NP_214634.1 15607262 886894 complement(145627..147771) 1 NC_000962.3 Rv0120c, (MTCI418B.02c), len: 714 aa. Probable fusA2 (alternate gene name: fus2), elongation factor G, highly similar to others e.g. EFG_ECOLI|P02996 elongation factor G (ef-g) from Escherichia coli (703 aa), FASTA scores: opt: 1049, E(): 0, (32.5% identity in 717 aa overlap). Also similar to fusA1|MTCY210.01 from Mycobacterium tuberculosis FASTA score: (39.1% identity in 299 aa overlap); and P30767|EFG_MYCLE elongation factor G (EF-G) from Mycobacterium leprae (701 aa), FASTA score: (31.7% identity in 710 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the GTP-binding elongation factor family, EF-G/EF-2 subfamily.; Probable elongation factor G FusA2 (EF-G) 147771 fusA2 886894 fusA2 Mycobacterium tuberculosis H37Rv Probable elongation factor G FusA2 (EF-G) NP_214634.1 145627 R 83332 CDS NP_214635.1 15607263 886892 complement(147908..148342) 1 NC_000962.3 Rv0121c, (MTCI418B.03c), len: 144 aa. Conserved protein, showing some similarity with others proteins from Mycobacterium tuberculosis e.g. Rv1155, Rv1875, Rv2074,etc.; hypothetical protein 148342 886892 Rv0121c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214635.1 147908 R 83332 CDS NP_214636.1 15607264 886888 148491..148859 1 NC_000962.3 Rv0122, (MTCI418B.04), len: 122 aa. Hypothetical unknown protein.; Hypothetical protein 148859 886888 Rv0122 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214636.1 148491 D 83332 CDS NP_214637.1 15607265 886887 148856..149224 1 NC_000962.3 Rv0123, (MTCI418B.05), len: 122 aa. Unknown protein.; hypothetical protein 149224 886887 Rv0123 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214637.1 148856 D 83332 CDS YP_177693.1 57116692 886883 149533..150996 1 NC_000962.3 Rv0124, (MTCI418B.06), len: 487 aa. PE_PGRS2, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1730, E(): 0,(60.7% identity in 504 aa overlap).; PE-PGRS family protein PE_PGRS2 150996 PE_PGRS2 886883 PE_PGRS2 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS2 YP_177693.1 149533 D 83332 CDS NP_214639.1 15607267 886924 151148..152215 1 NC_000962.3 Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa),FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa protein precursor from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable serine protease PepA (serine proteinase) (MTB32A) 152215 pepA 886924 pepA Mycobacterium tuberculosis H37Rv Probable serine protease PepA (serine proteinase) (MTB32A) NP_214639.1 151148 D 83332 CDS NP_214640.1 15607268 886881 152324..154129 1 NC_000962.3 Rv0126, (MTCI418B.08), len: 601 aa. TreS, trehalose synthase (see citation below), highly similar to others e.g. CAA04601.2|AJ001205 putative trehalose synthase from Streptomyces coelicolor (566 aa); S71450|1536814|BAA11303.1|D78198 trehalose synthase maltose-specific from Pimelobacter sp. strain R48 (573 aa). Also similar to MAL1_DROME|P07191 possible maltase precursor (508 aa), FASTA scores: opt: 807, E(): 0, (33.7% identity in 504 aa overlap); and similar to proteins associated with amino-acid transport e.g. Q64319 rat protein which stimulates transport of cystine and dibasic and neutral amino acids (683 aa), FASTA scores: opt: 839,E(): 0, (32.0% identity in 531 aa overlap). Also similar to several other Mycobacterium tuberculosis proteins e.g. Rv2471 FASTA score: (31.7% identity in 164 aa overlap).; Trehalose synthase TreS 154129 treS 886881 treS Mycobacterium tuberculosis H37Rv Trehalose synthase TreS NP_214640.1 152324 D 83332 CDS NP_214641.1 15607269 886880 154232..155599 1 NC_000962.3 Rv0127, (MTCI418B.09, MTCI5.01), len: 455 aa. Mak,maltokinase; highly similar to various proteins e.g. AJ0012|SCJ001205_4 hypothetical protein from Streptomyces coelicolor A3(2) (464 aa), FASTA scores: opt: 412, E(): 1.1e-19, (40.6% identity in 485 aa overlap); AJ0012|SCJ001206_5 hypothetical protein from Streptomyces coelicolor A3(2) (453 aa), FASTA scores: opt: 403, E(): 4.3 e-19, (36.5% identity in 455 aa overlap).; Maltokinase Mak 155599 mak 886880 mak Mycobacterium tuberculosis H37Rv Maltokinase Mak NP_214641.1 154232 D 83332 CDS NP_214642.1 15607270 886878 155667..156446 1 NC_000962.3 Rv0128, (MTCI5.02), len: 259 aa. Probable conserved transmembrane protein, with some similarity to Rv3064c and other bacterial proteins e.g. AAK85977.1|AE007957|AGR_C_254p from Agrobacterium tumefaciens (206 aa).; Probable conserved transmembrane protein 156446 886878 Rv0128 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214642.1 155667 D 83332 CDS YP_177694.1 57116693 886885 complement(156578..157600) 1 NC_000962.3 Rv0129c, (MT0137, MTCI5.03c), len: 340 aa. FbpC (alternate gene names: mpt45, 85C, fbpC2), secreted antigen 85c (fibronectin-binding protein C) (mycolyl transferase 85C) (see citations below), also highly similar to other Mycobacterial antigen precursors e.g. A85C_MYCLE|Q05862 antigen 85-c precursor (85c) from Mycobacterium leprae (333 aa), FASTA scores: opt: 1937, E(): 0, (81.4% identity in 333 aa overlap); etc.; Secreted antigen 85-C FbpC (85C) (antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) 157600 fbpC 886885 fbpC Mycobacterium tuberculosis H37Rv Secreted antigen 85-C FbpC (85C) (antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) YP_177694.1 156578 R 83332 CDS NP_214644.1 15607272 886876 157847..158302 1 NC_000962.3 Rv0130, (MTCI5.04), len: 151 aa. Probable htdZ,3-hydroxyl-thioester dehydratase. Forms single hot-dog fold, features R-specific hydratase motif, substrate unknown, forms homodimer. Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Similar to others e.g. AL096811|SCI30A_19 from Streptomyces coelicolor (153 aa), FASTA scores: opt: 639, E(): 0, (60.8% identity in 148 aa overlap).; Probable 3-hydroxyl-thioester dehydratase 158302 htdZ 886876 htdZ Mycobacterium tuberculosis H37Rv Probable 3-hydroxyl-thioester dehydratase NP_214644.1 157847 D 83332 CDS NP_214645.1 15607273 886874 complement(158315..159658) 1 NC_000962.3 Rv0131c, (MTCI5.05c), len: 447 aa. Probable fadE1,acyl-CoA dehydrogenase, similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase short-chain specific precursor (412 aa), FASTA scores: opt: 522, E(): 1.4e-23, (30.1% identity in 425 aa overlap). Also highly similar to MTCI5_28 from Mycobacterium tuberculosis.; Probable acyl-CoA dehydrogenase FadE1 159658 fadE1 886874 fadE1 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE1 NP_214645.1 158315 R 83332 CDS NP_214646.1 15607274 886877 complement(159700..160782) 1 NC_000962.3 Rv0132c, (MTCI5.06c), len: 360 aa. Putative fgd2,F420-dependent glucose-6-phosphate dehydrogenase, highly similar to many from Mycobacteria e.g. AAD38167|g5031431 from Mycobacterium chelonae. Also similar to MJ1534|Q58929 N5,N10-methylene tetrahydromethanopterin reductase from methanococcus jannaschii (342 aa), FASTA scores: opt: 285,E(): 7.9e-11, (28.4% identity in 292 aa overlap). And also similar to Rv0953c, Rv0791c, etc from Mycobacterium tuberculosis. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Putative F420-dependent glucose-6-phosphate dehydrogenase Fgd2 160782 fgd2 886877 fgd2 Mycobacterium tuberculosis H37Rv Putative F420-dependent glucose-6-phosphate dehydrogenase Fgd2 NP_214646.1 159700 R 83332 CDS NP_214647.1 15607275 886873 160869..161474 1 NC_000962.3 Rv0133, (MTCI5.07), len: 201 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Highly similar to others e.g. PUAC_STRLP|P13249 puromycyn N-acetyltransferase (199 aa),FASTA scores: opt: 341, E(): 1.8e-16, (33.3% identity in 201 aa overlap).; GCN5-related N-acetyltransferase 161474 886873 Rv0133 Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_214647.1 160869 D 83332 CDS NP_214648.1 15607276 886871 161771..162673 1 NC_000962.3 Rv0134, (MTCI5.08), len: 300 aa. Possible ephE,epoxide hydrolase (see citation below), similar to others e.g. Q39856 epoxide hydrolase (341 aa), FASTA scores: opt: 369, E(): 4.6e-17, (27.2% identity in 335 aa overlap); etc. Also similar to MTCY09F9.26c from Mycobacterium tuberculosis (29.5% identity in 346 aa overlap).; Possible epoxide hydrolase EphF (epoxide hydratase) (arene-oxide hydratase) 162673 ephF 886871 ephF Mycobacterium tuberculosis H37Rv Possible epoxide hydrolase EphF (epoxide hydratase) (arene-oxide hydratase) NP_214648.1 161771 D 83332 CDS NP_214649.1 15607277 886869 complement(162644..163249) 1 NC_000962.3 Rv0135c, (MTCI5.09c), len: 201 aa. Possible transcriptional regulator, weakly similar to others e.g. P32398|YHGD_BACSU hypothetical transcriptional regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 145,E(): 0.0012, (21.0% identity in 162 aa overlap).; Possible transcriptional regulatory protein 163249 886869 Rv0135c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214649.1 162644 R 83332 CDS NP_214650.1 15607278 886868 163366..164691 1 NC_000962.3 Rv0136, (MT0144, MTCI5.10), len: 441 aa. Probable cyp138, cytochrome P450 138, similar to others e.g. SLR0574|Q59990 from synechocystis SP. (444 aa), FASTA scores: opt: 315, E(): 1e-13, (25.7% identity in 416 aa overlap); etc. Also similar to MTV039_6 from Mycobacterium tuberculosis (472 aa), FASTA score: (38.2% identity in 442 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Probable cytochrome P450 138 Cyp138 164691 cyp138 886868 cyp138 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 138 Cyp138 NP_214650.1 163366 D 83332 CDS NP_214651.1 15607279 886865 complement(164712..165260) 1 NC_000962.3 Rv0137c, (MTCI5.11c), len: 182 aa. Probable msrA,peptide methionine sulfoxide reductase (See St. John et al., 2001), equivalent to CAC32179.1|AL583926 putative peptide methionine sulfoxide from Mycobacterium leprae (177 aa). Highly similar to others e.g. CAC18703.1|AL451182 putative peptide methionine sulfoxide reductase from Streptomyces coelicolor (172 aa); PMSR_SCHPO|Q09859 putative peptide methionine sulfoxide reductase from Streptomyces (187 aa), FASTA scores: opt: 468, E(): 9.9e-26, (45.6% identity in 158 aa overlap); etc. Belongs to the MsrA family.; Probable peptide methionine sulfoxide reductase MsrA (protein-methionine-S-oxide reductase) (peptide met(O) reductase) 165260 msrA 886865 msrA Mycobacterium tuberculosis H37Rv Probable peptide methionine sulfoxide reductase MsrA (protein-methionine-S-oxide reductase) (peptide met(O) reductase) NP_214651.1 164712 R 83332 CDS NP_214652.1 15607280 886863 165323..165826 1 NC_000962.3 Rv0138, (MTCI5.12), len: 167 aa. Conserved hypothetical protein, showing weak similarity to Q10827|YT10_MYCTU hypothetical 17.0 KDA protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 131, E(): 0.047, (31.15% identity in 106 aa overlap).; hypothetical protein 165826 886863 Rv0138 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214652.1 165323 D 83332 CDS NP_214653.1 15607281 886860 165827..166849 1 NC_000962.3 Rv0139, (MTCI5.13), len: 340 aa. Possible oxidoreductase, similar to others e.g. O34285|HPNA HPNA protein from Zymomonas mobilis (337 aa), FASTA scores: opt: 507, E (): 5.8e-27, (31.1% identity in 328 aa overlap); TRE_STRGR|P29782 dtdp-glucose 4,6-dehydratase (328 aa),FASTA scores: opt: 254, E(): 2.6e-10, (29.0% identity in 307 aa overlap).; Possible oxidoreductase 166849 886860 Rv0139 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214653.1 165827 D 83332 CDS NP_214654.1 15607282 886859 166910..167290 1 NC_000962.3 Rv0140, (MTCI5.14), len: 126 aa. Conserved protein,similar to others e.g. P74567|D90916_48 hypothetical 20.8 KDP protein from Synechocystis sp. (180 aa), FASTA scores: opt: 229, E(): 4.7e-10, (36.1% identity in 108 aa overlap). Also similar to Rv1056 and Rv1670 from Mycobacterium tuberculosis.; hypothetical protein 167290 886859 Rv0140 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214654.1 166910 D 83332 CDS NP_214655.1 15607283 886872 complement(167271..167681) 1 NC_000962.3 Rv0141c, (MTCI5.15c), len: 136 aa. Unknown protein.; hypothetical protein 167681 886872 Rv0141c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214655.1 167271 R 83332 CDS NP_214656.1 15607284 886858 167711..168637 1 NC_000962.3 Rv0142, (MTCI5.16), len: 308 aa. Conserved hypothetical protein, similar, except in N-terminus, to AB88922.1|AL353862 hypothetical protein SCE34.20 from Streptomyces coelicolor (326 aa).; hypothetical protein 168637 886858 Rv0142 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214656.1 167711 D 83332 CDS NP_214657.1 15607285 886856 complement(168704..170182) 1 NC_000962.3 Rv0143c, (MTCI5.17c), len: 492 aa. Probable conserved transmembrane protein, CIC family possibly involved in transport of chloride, similar to others and hypothetical proteins e.g. O28857 putative chloride channel from Archaeoglobus fulgidus (589 aa), FASTA scores: opt: 966, E(): 0, (37.7% identity in 453 aa overlap); YADQ_ECOLI|P37019 hypothetical 46.0 kDa protein (436 aa),FASTA scores: opt: 452, E(): 2.4e-20, (28.0% identity in 460 aa overlap).; Probable conserved transmembrane protein 170182 886856 Rv0143c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214657.1 168704 R 83332 CDS NP_214658.1 15607286 886854 170284..171126 1 NC_000962.3 Rv0144, (MTCI5.18), len: 280 aa. Probable transcriptional regulator, possibly TetR family. Has region similar to others e.g. Q59431|UIDR_ECOLI|GUSR|B1618|Z2623|ECS2326 UID operon repressor (GUS operon) from Escherichia coli strains K12 and O157:H7 (196 aa), FASTA scores: opt: 214, E(): 1.1e-06,(26.0% identity in 196 aa overlap). Contains probable helix-turn helix motif from aa 109-130 (Score 1463, +4.17 SD). Could belong to the TetR/AcrR family of transcriptional regulators.; Probable transcriptional regulatory protein (possibly TetR-family) 171126 886854 Rv0144 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (possibly TetR-family) NP_214658.1 170284 D 83332 CDS NP_214659.1 15607287 886851 171215..172168 1 NC_000962.3 Rv0145, (MTCI5.19), len: 317 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to many e.g. CAC32172.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (310 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc.; Possible S-adenosylmethionine-dependent methyltransferase 172168 886851 Rv0145 Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_214659.1 171215 D 83332 CDS NP_214660.1 15607288 886849 172211..173143 1 NC_000962.3 Rv0146, (MTCI5.20), len: 310 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to others e.g. AC30975.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (304 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc.; Possible S-adenosylmethionine-dependent methyltransferase 173143 886849 Rv0146 Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_214660.1 172211 D 83332 CDS NP_214661.1 15607289 886847 173238..174758 1 NC_000962.3 Rv0147, (MTCI5.21), len: 506 aa. Probable aldehyde dehydrogenase (NAD+) dependent, similar to others e.g. DHAP_RAT|P11883 aldehyde dehydrogenase (dimeric NADP-preferring) (452 aa), FASTA scores: opt: 1291, E(): 0,(43.9% identity in 453 aa overlap). Also similar to several Mycobacterium tuberculosis aldehyde dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 aldehyde dehydrogenases glutamic acid active site, and PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family.; Probable aldehyde dehydrogenase (NAD+) dependent 174758 886847 Rv0147 Mycobacterium tuberculosis H37Rv Probable aldehyde dehydrogenase (NAD+) dependent NP_214661.1 173238 D 83332 CDS NP_214662.1 15607290 886845 174833..175693 1 NC_000962.3 Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 175693 886845 Rv0148 Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_214662.1 174833 D 83332 CDS NP_214663.1 15607291 886843 175700..176668 1 NC_000962.3 Rv0149, (MTCI5.23), len: 322 aa. Possible quinone oxidoreductase, similar to others oxidoreductases e.g. Q08257 quinone oxidoreductase (329 aa), FASTA scores: opt: 397, E(): 3.2e-18, (28.4% identity in 328 aa overlap); SCHCOADH_4 from Streptomyces coelicolor. Also similar to many proteins from Mycobacterium tuberculosis. Contains PS01162 Quinone oxidoreductase / zeta-crystallin signature. Belongs to the zinc-containing alcohol dehydrogenase family, quinone oxidoreductase subfamily.; Possible quinone oxidoreductase (NADPH:quinone oxidoreductase) (zeta-crystallin) 176668 886843 Rv0149 Mycobacterium tuberculosis H37Rv Possible quinone oxidoreductase (NADPH:quinone oxidoreductase) (zeta-crystallin) NP_214663.1 175700 D 83332 CDS NP_214664.1 15607292 886840 complement(176665..176952) 1 NC_000962.3 Rv0150c, (MTCI5.24c), len: 95 aa. Conserved hypothetical protein, showing some similarity with C-terminus of O53949|Rv1800|MTV049.22 PPE-family protein from Mycobacterium tuberculosis (655 aa), FASTA score: (36.5% identity in 104 aa overlap).; hypothetical protein 176952 886840 Rv0150c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214664.1 176665 R 83332 CDS YP_177695.1 57116694 886857 complement(177543..179309) 1 NC_000962.3 Rv0151c, (MTCI5.25c), len: 588 aa. PE1, Member of the Mycobacterium tuberculosis PE family (see citation below), with N-terminal region similar to others e.g. MTV032_2 PE_PGRS family from Mycobacterium tuberculosis (468 aa), FASTA scores: opt: 1125, E(): 0, (46.3% identity in 456 aa overlap); MTCY493_24 from Mycobacterium tuberculosis FASTA score: (42.5% identity in 558 aa overlap). Also similar to upstream ORF MTCI5.26c FASTA score: (54.7% identity in 464 aa overlap). Also shows similarity to C-terminal part of some PPE family proteins e.g. MTV049_21 from Mycobacterium tuberculosis FASTA score: (41.5% identity in 591 aa overlap).; PE family protein PE1 179309 PE1 886857 PE1 Mycobacterium tuberculosis H37Rv PE family protein PE1 YP_177695.1 177543 R 83332 CDS YP_177696.1 57116695 886838 complement(179319..180896) 1 NC_000962.3 Rv0152c, (MTCI5.26c), len: 525 aa. PE2, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to ORF downstream Z92770|MTCI5_25 (588 aa),FASTA scores: opt: 1492, E(): 0, (54.7% identity in 464 aa overlap); and to many other PE family type members. Predicted to be an outer membrane protein (See Song et al.,2008).; PE family protein PE2 180896 PE2 886838 PE2 Mycobacterium tuberculosis H37Rv PE family protein PE2 YP_177696.1 179319 R 83332 CDS NP_214667.1 15607295 886842 complement(181155..181985) 1 NC_000962.3 Rv0153c, (MTCI5.27c), len: 276 aa. PtbB (alternate gene name: MPtpB), protein-tyrosine-phosphatase (see citation below), showing some similarity to several protein-tyrosine phosphatases, polyketide synthase and aminotransferase e.g. Q05918|IPHP_NOSCO|IPH protein-tyrosine-phosphatase precursor from Nostoc commune (294 aa), FASTA scores: opt: 150, E(): 0.0096, (26.8% identity in 269 aa overlap); etc. Supposedly a secreted protein. Potent and selective inhibitor is an isoxazole compound (See Seollner et al., 2007).; Phosphotyrosine protein phosphatase PTPB (protein-tyrosine-phosphatase) (PTPase) 181985 ptbB 886842 ptbB Mycobacterium tuberculosis H37Rv Phosphotyrosine protein phosphatase PTPB (protein-tyrosine-phosphatase) (PTPase) NP_214667.1 181155 R 83332 CDS NP_214668.1 15607296 886836 complement(181987..183198) 1 NC_000962.3 Rv0154c, (MTCI5.28c), len: 403 aa. Probable fadE2,acyl-CoA dehydrogenase, similar to many e.g. C-terminal region of O01590 acyl-CoA dehydrogenase (974 aa), FASTA scores: opt: 1150, E(): 0, (50.0% identity in 402 aa overlap); ACDS_MEGEL|Q06319 acyl-CoA dehydrogenase (short-chain) (383 aa), FASTA score: (35.0% identity in 306 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE2 183198 fadE2 886836 fadE2 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE2 NP_214668.1 181987 R 83332 CDS NP_214669.1 15607297 886832 183622..184722 1 NC_000962.3 Rv0155, (MTCI5.29), len: 366 aa. Probable pntAa,first part of NAD(P) transhydrogenase subunit alpha,similar to N-terminus of others e.g. PNTA_ECOLI|P07001|P76888|B1603 NAD (P) transhydrogenase subunit alpha from Escherichia coli strain K12 (510 aa),FASTA scores: opt: 921, E(): 0, (42.1% identity in 361 aa overlap); proton-translocating nicotinamide nucleotide transhydrogenase subunit PNTAA.; Probable NAD(P) transhydrogenase (subunit alpha) PntAa [first part; catalytic part] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) 184722 pntAa 886832 pntAa Mycobacterium tuberculosis H37Rv Probable NAD(P) transhydrogenase (subunit alpha) PntAa [first part; catalytic part] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) NP_214669.1 183622 D 83332 CDS NP_214670.1 15607298 886890 184723..185055 1 NC_000962.3 Rv0156, (MTCI5.30), len: 110 aa. Probable pntAb,second part of NAD(P) transhydrogenase subunit alpha,integral membrane protein, similar to C-terminus of others e.g. Q59764 nicotinamide nucleotide transhydrogenase subunit PNTAB (139 aa), FASTA scores: opt: 247, E(): 1.9e-11, (45.5% identity in 88 aa overlap).; Probable NAD(P) transhydrogenase (subunit alpha) PntAb [second part; integral membrane protein] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) 185055 pntAb 886890 pntAb Mycobacterium tuberculosis H37Rv Probable NAD(P) transhydrogenase (subunit alpha) PntAb [second part; integral membrane protein] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) NP_214670.1 184723 D 83332 CDS NP_214671.1 15607299 886830 185052..186479 1 NC_000962.3 Rv0157, (MTCI5.31), len: 475 aa. Probable pntB,pyridine nucleotide transhydrogenase (nicotinamide nucleotide transhydrogenase) subunit beta, integral membrane protein, similar to others e.g. Q59763 proton-translocating nicotinamide nucleotide transhydrogenase subunit beta from hodospirillum rubrum (464 aa), FASTA scores: opt: 1344, E(): 0, (46.4% identity in 472 aa overlap); P07002|PNTB_ECOLI|P76890|PNTB|B1602|Z2597|ECS2308 NAD(P) transhydrogenase subunit beta from Escherichia coli strains K12 and O157:H7 (462 aa).; Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit beta) (nicotinamide nucleotide transhydrogenase subunit beta) 186479 pntB 886830 pntB Mycobacterium tuberculosis H37Rv Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit beta) (nicotinamide nucleotide transhydrogenase subunit beta) NP_214671.1 185052 D 83332 CDS YP_007408797.1 448814924 3205086 complement(186495..186623) 1 NC_000962.3 Rv0157A, len: 42 aa. Conserved protein, showing similarity to C-terminal part (aa 186-220) of O53976|Rv1975|MTV051.13 conserved hypothetical protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 173, E(): 3e-06, (62.5% identity in 40 aa overlap).; hypothetical protein 186623 3205086 Rv0157A Mycobacterium tuberculosis H37Rv hypothetical protein YP_007408797.1 186495 R 83332 CDS NP_214672.1 15607300 886828 186785..187429 1 NC_000962.3 Rv0158, (MTV032.01), len: 214 aa. Probable transcriptional regulator, possibly TetR family, showing weak similarity to various transcriptional activators and repressors e.g. P32398|YIXD_BACSU|YHGD hypothetical transcriptional regulatory protein from Bacillus subtilis (191 aa), FASTA scores: opt:172, E(): 2.4e-05, (23.0% identity in 191 aa overlap). Contains helix-turn-helix motif at aa 32-53 (Score 1296, +3.60 SD). Could belong to the TetR/AcrR family of transcriptional regulators.; Probable transcriptional regulatory protein (possibly TetR-family) 187429 886828 Rv0158 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (possibly TetR-family) NP_214672.1 186785 D 83332 CDS YP_177697.1 57116696 886826 complement(187433..188839) 1 NC_000962.3 Rv0159c, (MTV032.02c), len: 468 aa. PE3, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. O06828 from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1163, E(): 0, (45.8% identity in 467 aa overlap). Also highly similar to upstream MTV032_3, and to MTCI5_25,MTCI5_26, MTV049_ 21, MTCY1A10_26, etc.; PE family protein PE3 188839 PE3 886826 PE3 Mycobacterium tuberculosis H37Rv PE family protein PE3 YP_177697.1 187433 R 83332 CDS YP_177698.1 57116697 886825 complement(188931..190439) 1 NC_000962.3 Rv0160c, (MTV032.03c), len: 502 aa. PE4, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. Z92770|MTCI5_26c from Mycobacterium tuberculosis (525 aa),FASTA scores: opt: 816, E(): 0, (41.4% identity in 367 aa overlap); C-terminal region of O06801|RV1768|MTCY28.34 from Mycobacterium tuberculosis (618 aa), FASTA scores: opt: 417, E(): 6.7e-18, (53.5% identity in 142 aa overlap). Also highly similar to downstream ORF MTV032_2.; PE family protein PE4 190439 PE4 886825 PE4 Mycobacterium tuberculosis H37Rv PE family protein PE4 YP_177698.1 188931 R 83332 CDS NP_214675.1 15607303 886835 190607..191956 1 NC_000962.3 Rv0161, (MTCI28.01, MTV032.04), len: 449 aa. Possible oxidoreductase, similar to hypothetical proteins and various oxidoreductases e.g. AIP2_YEAST|P46681 actin interacting protein 2 (530 aa), FASTA scores: opt: 356, E (): 0, (33.3% identity in 357 aa overlap); DLD1_YEAST|P32891 d-lactate dehydrogenase (cytochrome) (587 aa), FASTA scores: opt: 311, E(): 2.5e-20, (27.9% identity in 366 aa overlap). Also similar to other Mycobacteria proteins e.g. MTCY339.30c from Mycobacterium tuberculosis FASTA score: (29.4% identity in 357 aa overlap); MLCL622.30c from Mycobacterium tuberculosis (449 aa).; Possible oxidoreductase 191956 886835 Rv0161 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214675.1 190607 D 83332 CDS YP_177699.1 57116698 886824 complement(191984..193135) 1 NC_000962.3 Rv0162c, (MTCI28.02c), len: 383 aa. Probable adhE1,zinc-type alcohol dehydrogenase, similar to others e.g. ADH_MACMU|P28469 alcohol dehydrogenase alpha chain (374 aa), FASTA scores: opt: 619, E(): 0, (34.7% identity in 363 aa overlap). Also similar to other alcohol dehydrogenases from Mycobacterium tuberculosis e.g. MTCY369.06c FASTA score: (34.0% identity in 365 aa overlap), MTV022_9 FASTA score: (35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family,class-I subfamily. Cofactor: zinc.; Probable zinc-type alcohol dehydrogenase (E subunit) AdhE1 193135 adhE1 886824 adhE1 Mycobacterium tuberculosis H37Rv Probable zinc-type alcohol dehydrogenase (E subunit) AdhE1 YP_177699.1 191984 R 83332 CDS NP_214677.1 15607305 886821 193117..193572 1 NC_000962.3 Rv0163, (MTCI28.03), len: 151 aa. Conserved protein,similar to others e.g. Q44017 hypothetical 16.6 KDA protein in GBD 5'region (ORF6)from Alcaligenes eutrophus (145 aa),FASTA scores: opt: 155, E(): 0.0002, (26.6% identity in 139 aa overlap). Also weak similarity with MTV008.31c|Rv2475c|B70867 from Mycobacterium tuberculosis (138 aa).; hypothetical protein 193572 886821 Rv0163 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214677.1 193117 D 83332 CDS YP_177617.1 57116699 886267 193626..194111 1 NC_000962.3 Rv0164, (MTCI28.04), len: 161 aa. TB18.5, conserved protein, equivalent to CAB08818.1|Z95398 hypothetical protein from Mycobacterium leprae (156 aa) FASTA scores: opt: 762, E(): 0, (76.3% identity in 152 aa overlap). Some similarity to Rv2185c, Rv0854, Rv0857 from Mycobacterium tuberculosis. Alternative start codon has been suggested. 3' part corrected since first submission (-24 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Conserved protein TB18.5 194111 TB18.5 886267 TB18.5 Mycobacterium tuberculosis H37Rv Conserved protein TB18.5 YP_177617.1 193626 D 83332 CDS YP_177700.2 448824738 886818 complement(194144..194815) 1 NC_000962.3 Rv0165c, (MTCI28.05c), len: 223 aa. Probable mce1R,transcriptional regulator, GntR family (See Casali et al.,2006), showing some similarity to several e.g. NTRA_CHELE|P54988 nta operon transcriptional regulator (231 aa), FASTA scores: opt: 154, E(): 0.00058, (32.0% identity in 125 aa overlap); P46833|GNTR_BACLI gluconate operon transcriptional repressor from Bacillus licheniformis (243 aa); GNTR_BACSU gluconate operon repressor from Bacillus subtilis (243 aa). Also similar to Rv0043c from Mycobacterium tuberculosis. Seems to belong to the GntR family of transcriptional regulators. Start changed since first submission (-41 aa).; Probable transcriptional regulatory protein Mce1R (probably GntR-family) 194815 mce1R 886818 mce1R Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein Mce1R (probably GntR-family) YP_177700.2 194144 R 83332 CDS NP_214680.1 15607307 886822 194993..196657 1 NC_000962.3 Rv0166, (MTCI28.06), len: 554 aa. Probable fadD5,fatty-acid-CoA synthetase, similar to many eg LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 612, E(): 0, (29.4% identity in 534 aa overlap). Also similar to many other fatty-acid-CoA ligases from Mycobacterium tuberculosis e.g. MTCY07A7.11c FASTA score: (35.3% identity in 487 aa overlap), MTV013_10,MTY25D10_30, etc. Contains PS00455 putative AMP-binding domain signature.; Probable fatty-acid-CoA ligase FadD5 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 196657 fadD5 886822 fadD5 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD5 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_214680.1 194993 D 83332 CDS NP_214681.1 15607308 886816 196861..197658 1 NC_000962.3 Rv0167, (MTCI28.07), len: 265 aa. YrbE1A, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar or similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 328,E(): 1.8e-15, (26.6% identity in 244 aa overlap); etc.; Conserved integral membrane protein YrbE1A 197658 yrbE1A 886816 yrbE1A Mycobacterium tuberculosis H37Rv Conserved integral membrane protein YrbE1A NP_214681.1 196861 D 83332 CDS NP_214682.1 15607309 886812 197660..198529 1 NC_000962.3 Rv0168, (MTCI28.08), len: 289 aa. YrbE1B, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 223,E(): 7.6e-07, (23.7% identity in 257 aa overlap); etc.; Conserved integral membrane protein YrbE1B 198529 yrbE1B 886812 yrbE1B Mycobacterium tuberculosis H37Rv Conserved integral membrane protein YrbE1B NP_214682.1 197660 D 83332 CDS YP_177701.1 57116701 886823 198534..199898 1 NC_000962.3 Rv0169, (MTCI28.09), len: 454 aa. Mce1A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); AAA92845.1|U26018 mce gene product from Mycobacterium avium (88 aa) (similarity on C-terminus); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Note that equivalent, but longer 22 aa, to P72013|CAA50257.1|X70901 Mcep protein from Mycobacterium tuberculosis (432 aa). Contains a very hydrophobic region around residues 20-35. Note that previously known as mce1. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce1A 199898 mce1A 886823 mce1A Mycobacterium tuberculosis H37Rv Mce-family protein Mce1A YP_177701.1 198534 D 83332 CDS NP_214684.1 15607311 886810 199895..200935 1 NC_000962.3 Rv0170, (MTCI28.10), len: 346 aa. Mce1B (alternate gene name: mceD); belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below),highly similar to Mycobacterium tuberculosis proteins O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains hydrophobic region in N-terminal 30 residues. In Escherichia coli,N-terminal part is functional and directs export of a leaderless beta-lactamase into the periplasm (see Chubb et al., 1998). Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce1B 200935 mce1B 886810 mce1B Mycobacterium tuberculosis H37Rv Mce-family protein Mce1B NP_214684.1 199895 D 83332 CDS NP_214685.1 15607312 886808 200932..202479 1 NC_000962.3 Rv0171, (MTCI28.11), len: 515 aa. Mce1C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Weakly similar to downstream ORF Rv0172|MTCI28.12|mce1D (530 aa), FASTA score: (24.6% identity in 552 aa overlap). Contains possible signal sequence and highly proline-rich C-terminus. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce1C 202479 mce1C 886808 mce1C Mycobacterium tuberculosis H37Rv Mce-family protein Mce1C NP_214685.1 200932 D 83332 CDS NP_214686.1 15607313 886807 202476..204068 1 NC_000962.3 Rv0172, (MTCI28.12), len: 530 aa. Mce1D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce1D 204068 mce1D 886807 mce1D Mycobacterium tuberculosis H37Rv Mce-family protein Mce1D NP_214686.1 202476 D 83332 CDS NP_214687.1 15607314 886804 204065..205237 1 NC_000962.3 Rv0173, (MTCI28.13), len: 390 aa. Possible lprK (alternate gene name: mce1E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains PS00013 prokaryotic membrane lipoprotein lipid attachment site.; Possible Mce-family lipoprotein LprK (Mce-family lipoprotein Mce1E) 205237 lprK 886804 lprK Mycobacterium tuberculosis H37Rv Possible Mce-family lipoprotein LprK (Mce-family lipoprotein Mce1E) NP_214687.1 204065 D 83332 CDS NP_214688.1 15607315 886820 205231..206778 1 NC_000962.3 Rv0174, (MTCI28.14), len: 515 aa. Mce1F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Predicted to be an outer membrane protein (See Song et al.,2008).; Mce-family protein Mce1F 206778 mce1F 886820 mce1F Mycobacterium tuberculosis H37Rv Mce-family protein Mce1F NP_214688.1 205231 D 83332 CDS NP_214689.3 448824739 886801 206814..207455 1 NC_000962.3 Rv0175, (MTCI28.15), len: 213 aa. Probable conserved Mce-associated membrane protein, equivalent, but longer in N-terminus, to CAC32127.1|AL583926 possible membrane protein from Mycobacterium leprae (182 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Contains two 12 residue direct repeats at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved Mce associated membrane protein 207455 886801 Rv0175 Mycobacterium tuberculosis H37Rv Probable conserved Mce associated membrane protein NP_214689.3 206814 D 83332 CDS NP_214690.1 15607317 886799 207452..208420 1 NC_000962.3 Rv0176, (MTCI28.16), len: 322 aa. Probable conserved Mce-associated transmembrane protein. Contains short region of similarity to PRA_MYCLE|P41484 proline-rich antigen (36 kDa antigen) from Mycobacterium leprae (249 aa) (outside the proline-rich region), FASTA scores: opt: 165, E(): 2.9e-05, (40.0% identity in 65 aa overlap). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv3493c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved Mce associated transmembrane protein 208420 886799 Rv0176 Mycobacterium tuberculosis H37Rv Probable conserved Mce associated transmembrane protein NP_214690.1 207452 D 83332 CDS NP_214691.1 15607318 886795 208417..208971 1 NC_000962.3 Rv0177, (MTCI28.17), len: 184 aa. Probable conserved Mce-associated protein, equivalent to CAC32129.1|AL583926 conserved membrane protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv1973, Rv3493c,etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved Mce associated protein 208971 886795 Rv0177 Mycobacterium tuberculosis H37Rv Probable conserved Mce associated protein NP_214691.1 208417 D 83332 CDS NP_214692.1 15607319 886814 208938..209672 1 NC_000962.3 Rv0178, (MTCI28.18), len: 244 aa. Probable conserved Mce-associated membrane protein, highly similar in C-terminus to CAC32130.1|AL583926 putative secreted protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Note that there is a 10 aa overlap with the upstream ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved Mce associated membrane protein 209672 886814 Rv0178 Mycobacterium tuberculosis H37Rv Probable conserved Mce associated membrane protein NP_214692.1 208938 D 83332 CDS NP_214693.1 15607320 886796 complement(209703..210812) 1 NC_000962.3 Rv0179c, (MTCI28.19c), len: 369 aa. Possible lprO,lipoprotein (visibly not conserved). Contains possible N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Possible lipoprotein LprO 210812 lprO 886796 lprO Mycobacterium tuberculosis H37Rv Possible lipoprotein LprO NP_214693.1 209703 R 83332 CDS NP_214694.1 15607321 886792 complement(210892..212250) 1 NC_000962.3 Rv0180c, (MTCI28.20c), len: 452 aa. Probable conserved transmembrane protein, equivalent to CAC32132.1|AL583926 probable conserved membrane protein from Mycobacterium leprae (465 aa). Shows some similarity with others membrane proteins e.g. AL096849|SCI11_29 from Streptomyces coelicolor (354 aa), FASTA scores: opt: 190,E(): 0.00067, (25.9% identity in 409 aa overlap).; Probable conserved transmembrane protein 212250 886792 Rv0180c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214694.1 210892 R 83332 CDS NP_214695.1 15607322 886788 complement(212277..213011) 1 NC_000962.3 Rv0181c, (MTCI28.21c), len: 244 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. YHHW_ECOLI|P46852 hypothetical 26.3 kd protein from Escherichia coli (231 aa), FASTA scores: opt: 479, E(): 1.2e-29, (37.3% identity in 233 aa overlap); P73623|SLL1773 hypothetical 25.7 kDa protein from Synechocystis sp. strain PCC 6803 (232 aa), FASTA score: (39.1% identity in 233 aa overlap).; hypothetical protein 213011 886788 Rv0181c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214695.1 212277 R 83332 CDS NP_214696.1 15607323 886786 complement(213028..214140) 1 NC_000962.3 Rv0182c, (MTCI28.22c), len: 370 aa (start site uncertain; first of several possibles was chosen, but note that this overlaps the upstream ORF). Probable sigG,alternative RNA polymerase sigma subunit (see citations below), similar to many e.g. Q45585|SIGW_BACSU RNA polymerase sigma factor from Bacillus subtilis (187 aa). Also similar to nine other ECF sigma factors from Mycobacterium tuberculosis e.g. Rv1221, Rv0735, etc. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 205-226 (Score 1181, +3.21 SD). Belongs to the sigma-70 factor family, ECF subfamily.; Probable alternative RNA polymerase sigma factor SigG (RNA polymerase ECF type sigma factor) 214140 sigG 886786 sigG Mycobacterium tuberculosis H37Rv Probable alternative RNA polymerase sigma factor SigG (RNA polymerase ECF type sigma factor) NP_214696.1 213028 R 83332 CDS NP_214697.2 57116702 886785 214088..214927 1 NC_000962.3 Rv0183, (MTCI28.23), len: 279 aa. Possible lysophospholipase, similar to several (especially eukaryotic enzymes, weaker with Escherichia coli), e.g. U67963|HSU67963_1 Human lysophospholipase homolog from Homo sapiens (313 aa), FASTA scores: opt: 569, E(): 2.6e-29,(37.1% identity in 259 aa overlap); P07000|PLDB_ECOLI lysophospholipase L2 from Escherichia coli (165 aa), FASTA scores: opt: 219, E(): 0.00012. Start changed based on similarity to AE001997_8 from Deinococcus radiodurans (282 aa), FASTA scores: opt: 510, E(): 1.4e-25, (34.8% identity in 282 aa overlap). Also shows some similarity to epoxide hydrolases from Mycobacterium tuberculosis e.g. Rv1938 FASTA score: (30.7% identity in 114 aa overlap); and O07214|YR15_MYCTU|Rv2715|MT2788|MTCY05A6.36 (341 aa).; Possible lysophospholipase 214927 886785 Rv0183 Mycobacterium tuberculosis H37Rv Possible lysophospholipase NP_214697.2 214088 D 83332 CDS NP_214698.1 15607325 886806 214969..215718 1 NC_000962.3 Rv0184, (MTCI28.24), len: 249 aa. Conserved hypothetical protein, equivalent to CAC32136.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (249 aa); and C-terminus highly similar to CAB08793.1|Z95398 conserved hypothetical protein from Mycobacterium leprae (145 aa), FASTA scores: E(): 0, (75.2 identity in 145 aa overlap). Also similar to 049841|SCE9_39|T36358 hypothetical protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 231,E(): 8.1e-08, (30.4% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 215718 886806 Rv0184 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214698.1 214969 D 83332 CDS NP_214699.1 15607326 886782 215715..216224 1 NC_000962.3 Rv0185, (MTCI28.25a), len: 169 aa. Conserved hypothetical protein, equivalent to CAB08794.1|Z95398|MLCL622_2 from Mycobacterium leprae (168 aa), FASTA scores: opt: 861, E(): 0, (76.4% identity in 165 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 216224 886782 Rv0185 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214699.1 215715 D 83332 CDS NP_214700.1 15607327 886780 216269..218344 1 NC_000962.3 Rv0186, (MTCI28.25b), len: 691 aa. Probable bglS,beta-glucosidase, highly similar to many e.g. BGLS_AGRTU|P27034 beta-glucosidase from Agrobacterium tumefaciens (818 aa), FASTA scores: opt: 643, E(): 0,(32.5% identity in 842 aa overlap). Seems to belong to family 3 of glycosyl hydrolases.; Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase) 218344 bglS 886780 bglS Mycobacterium tuberculosis H37Rv Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase) NP_214700.1 216269 D 83332 CDS YP_004837046.2 448824740 14515880 complement(218390..218551) 1 NC_000962.3 Rv0186A, len: 53 aa. MymT, metallothionein,equivalent to MAV_4993|A0QMH5 hypothetical protein from Mycobacterium avium (strain 104) (51 aa), and MAP_3626c|Q73TU2 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (51 aa), FASTA scores: opt: 312, E(): 4.6e-17, (81.2% identity in 48 aa overlap).; Metallothionein, MymT 218551 mymT 14515880 mymT Mycobacterium tuberculosis H37Rv Metallothionein, MymT YP_004837046.2 218390 R 83332 CDS NP_214701.1 15607328 886779 218705..219367 1 NC_000962.3 Rv0187, (MTCI28.26), len: 220 aa. Probable O-methyltransferase, similar to many e.g. AB93458.1|AL357591 putative O-methyltransferase from Streptomyces coelicolor (223 aa); MDMC_STRMY|Q00719 O-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores: opt: 327, E(): 2.4e-17, (35.9% identity in 192 aa overlap). Also similar to Rv1703c, Rv1220c from Mycobacterium tuberculosis.; Probable O-methyltransferase 219367 886779 Rv0187 Mycobacterium tuberculosis H37Rv Probable O-methyltransferase NP_214701.1 218705 D 83332 CDS NP_214702.1 15607329 886776 219486..219917 1 NC_000962.3 Rv0188, (MTCI28.27), len: 143 aa. Probable conserved transmembrane protein, similar to T35347|4835334|CAB42956.1|AL049863|SC5H1_31 probable membrane protein from Streptomyces coelicolor (147 aa),FASTA scores: opt: 326, E(): 6.5e-15, (36.2% identity in 141 aa overlap); N-terminus of P80185|MTRC_METTH tetrahydromethanopterin S-methyltransferase subunit C from Methanobacterium thermoautotrophicum strain Marburg/DSM 2133 (266 aa), FASTA scores: opt: 125, E(): 0.033, (31.6% identity in 98 aa overlap). Also similar to Rv3635 from Mycobacterium tuberculosis.; Probable conserved transmembrane protein 219917 886776 Rv0188 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214702.1 219486 D 83332 CDS NP_214703.1 15607330 886774 complement(219996..221723) 1 NC_000962.3 Rv0189c, (MTCI28.28c), len: 575 aa. Probable ilvD,dihydroxy-acid dehydratase, similar to many e.g. ILVD_LACLA|Q02139 dihydroxy-acid dehydratase (dad) from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (570 aa), FASTA scores: opt: 1605, E(): 0, (46.0% identity in 561 aa overlap). Also similar to ML2608|MLCL622.06c|O06069|ILVD_MYCLE dihydroxy-acid dehydratase from Mycobacterium leprae (564 aa). Contains PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. Belongs to the ILVD / EDD family. Cofactor: binds 1 4FE-4S cluster (potential).; Probable dihydroxy-acid dehydratase IlvD (dad) 221723 ilvD 886774 ilvD Mycobacterium tuberculosis H37Rv Probable dihydroxy-acid dehydratase IlvD (dad) NP_214703.1 219996 R 83332 CDS NP_214704.1 15607331 886772 221871..222161 1 NC_000962.3 Rv0190, (MTCI28.29), len: 96 aa. Conserved protein,highly similar to several hypothetical proteins e.g. SYCSLRA_35|Q55554|SLL0176 hypothetical 18.9 kDa protein from Synechocystis (167 aa), FASTA scores: opt: 237, E(): 5.8e-16, (39.4% identity in 94 aa overlap). Also highly similar to Z95398|MLCL622_7|O06070 from Mycobacterium leprae (135 aa), FASTA score: (82.6% identity in 92 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0967, Rv0030, Rv1766 (42.5% identity in 80 aa overlap).; hypothetical protein 222161 886772 Rv0190 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214704.1 221871 D 83332 CDS NP_214705.1 15607332 886770 222289..223530 1 NC_000962.3 Rv0191, (MTCI28.30), len: 413 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug,similar to several hypothetical proteins e.g. YDEA_ECOLI|P31122 hypothetical 42.5 kd protein from Escherichia coli (396 aa), FASTA scores: opt: 475, E(): 4.2e-33, (29.7% identity in 381 aa overlap); and to several chloramphenicol resistance proteins e.g. CMLR_STRLI|P31141 chloramphenicol resistance protein from Streptomyces lividans (392 aa), FASTA scores: opt: 394, E(): 6.7e-12,(28.2% identity in 383 aa overlap). Also similar to SVU09991_1 from Mycobacterium tuberculosis.; Probable conserved integral membrane protein 223530 886770 Rv0191 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_214705.1 222289 D 83332 CDS NP_214706.1 15607333 886768 223564..224664 1 NC_000962.3 Rv0192, (MTCI28.31), len: 366 aa. Conserved hypothetical protein. Has Gly- Arg-rich region followed by highly Pro-rich repetitive region near N-terminus. Similar in C-terminus to other hypothetical proteins e.g. Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa), FASTA scores: opt: 375, E(): 3.2e-24, (36.1% identity in 255 aa overlap); YV09_MYCTU|Q11149|cY20G9.09 hypothetical 47.9 kDa protein from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 330, E(): 3.2e-13, (35.1% identity in 271 aa overlap). Also similar to Rv0116c, Rv1433, Rv2518c, Rv0483 from Mycobacterium tuberculosis.; hypothetical protein 224664 886768 Rv0192 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214706.1 223564 D 83332 CDS YP_177618.1 57116703 3205105 223607..223909 1 NC_000962.3 Rv0192A, len: 100 aa. Probable N-terminal part of Rv0192, which is member of family P5.17 with Rv0116c,Rv1433, Rv2518c, Rv0483. These are all predicted to be exported/membrane proteins. Rv0192A has typical N-terminal signal peptide which is functional and was identified by PhoA fusion screens: O52054 PGB14T-O1 precursor (fragment 45 AA) (see Chubb et al., 1998). Since Rv0192 misses a signal peptide this suggests that there is a frameshift in the region of the overlap with Rv0192 but none found on reinspection of sequence.; Conserved secreted protein 223909 3205105 Rv0192A Mycobacterium tuberculosis H37Rv Conserved secreted protein YP_177618.1 223607 D 83332 CDS NP_214707.1 15607334 886764 complement(224724..226571) 1 NC_000962.3 Rv0193c, (MTV033.01c-MTCI28.32), len: 615 aa. Hypothetical unknown protein.; Hypothetical protein 226571 886764 Rv0193c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214707.1 224724 R 83332 CDS NP_214708.1 15607335 886790 226878..230462 1 NC_000962.3 Rv0194, (MTV033.02), len: 1194 aa. Probable multidrug efflux pump (See Danilchanka et al., 2008),highly similar to many e.g. U62129|STU62129_2|T30293 ABC transport protein homolog from Salmonella typhi (1218 aa),FASTA scores: opt: 1116, E(): 0, (36.3% identity in 1209 aa overlap); CAB66302.1|AL136519 ABC transporter protein ATP-binding component from Streptomyces coelicolor (1243 aa); I84547 mdl protein from Escherichia coli (1143 aa); etc. Also similar to MTCY50_9 and MTCY50_10 from Mycobacterium tuberculosis, FASTA score: (33.8% identity in 574 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop) and one PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Alternative start possible at 1823 but no RBS.; Probable transmembrane multidrug efflux pump 230462 886790 Rv0194 Mycobacterium tuberculosis H37Rv Probable transmembrane multidrug efflux pump NP_214708.1 226878 D 83332 CDS NP_214709.1 15607336 886762 230899..231534 1 NC_000962.3 Rv0195, (MTV033.03), len: 211 aa. Possible two-component response regulator, luxR family, similar to many e.g. U00008|ECOHU49_15 regulatory protein narP from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 232, E(): 7.3e-09, (29.2% identity in 219 aa overlap). Start chosen by similarity. Contains probable helix-turn-helix motif at aa 166-187 (Score 1164, +3.15 SD).; Possible two component transcriptional regulatory protein (probably LuxR-family) 231534 886762 Rv0195 Mycobacterium tuberculosis H37Rv Possible two component transcriptional regulatory protein (probably LuxR-family) NP_214709.1 230899 D 83332 CDS NP_214710.1 15607337 886760 231647..232231 1 NC_000962.3 Rv0196, (MTV033.04), len: 194 aa. Possible transcriptional regulatory protein, similar to two Bacillus subtilis regulators: P42105|YXAF_BACSU hypothetical 21.0 kDa protein (191 aa), FASTA scores: opt: 323, E(): 2.1e-15,(30.9% identity in 181 aa overlap); and Z99105|BSUB0002_9 negative regulator of the lincomycin operon (188 aa), FASTA scores: opt: 255, E(): 1e-10, (25.9 identity in 185 aa overlap).; Possible transcriptional regulatory protein 232231 886760 Rv0196 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214710.1 231647 D 83332 CDS NP_214711.1 15607338 886758 232231..234519 1 NC_000962.3 Rv0197, (MTV033.05), len: 762 aa. Possible oxidoreductase, similar to others e.g. 9948789|AAG06102.1|AE004699_7|B83307 probable molybdopterin oxidoreductase from Pseudomonas aeruginosa strain PAO1 (769 aa); 5441785|CAB46809.1|AL096811|T36812 probable dehydrogenase from Streptomyces coelicolor (747 aa), FASTA scores: opt: 617, E(): 9.8e-30, (29.9% identity in 762 aa overlap); BAB04334.1|AP001509 assimilatory nitrate reductase (catalytic subunit) from Bacillus halodurans (743 aa); etc.; Possible oxidoreductase 234519 886758 Rv0197 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214711.1 232231 D 83332 CDS NP_214712.1 15607339 886755 complement(234516..236507) 1 NC_000962.3 Rv0198c, (MTV033.06c), len: 663 aa. Probable zmp1,zinc metalloprotease, equivalent to Z95398|MLCL622.12c from Mycobacterium leprae (667 aa), FASTA scores: opt: 3710,E(): 0, (80.8 % identity in 667 aa overlap). Also similar to many other metalloproteases e.g. members of the eukaryotic neprilysin family: P08473|NEP_HUMAN neprilysin (749 aa), FASTA scores: opt: 872, E(): 0, (31.1% identity in 692 aa overlap); Q07744|PEPO_LACLA neutral endopeptidase from Lactococcus lactis (626 aa), FASTA scores: opt: 862,E(): 0, (30.0% identity in 654 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Belongs to peptidase family M13 (zinc metalloprotease); also known as the neprilysin subfamily.; Probable zinc metalloprotease Zmp1 236507 zmp1 886755 zmp1 Mycobacterium tuberculosis H37Rv Probable zinc metalloprotease Zmp1 NP_214712.1 234516 R 83332 CDS NP_214713.1 15607340 886753 236550..237209 1 NC_000962.3 Rv0199, (MTV033.07), len: 219 aa. Probable conserved membrane protein, equivalent to Z95398|MLCL622.13 from Mycobacterium leprae (224 aa), FASTA scores: opt: 920, E(): 0, (67.7% identity in 220 aa overlap). Also some similarity to Mce-associated membrane proteins from Mycobacterium tuberculosis e.g. Rv0178, Rv0175, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved membrane protein 237209 886753 Rv0199 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214713.1 236550 D 83332 CDS NP_214714.1 15607341 886803 237206..237895 1 NC_000962.3 Rv0200, (MTV033.08), len: 229 aa. Possible conserved transmembrane protein, equivalent to Z95398|MLCL622.14 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1147,E(): 0, (74.7% identity in 229 aa overlap). Also some similarity to Rv1973 from Mycobacterium tuberculosis (160 aa); and Rv1362c|Z75555|MTCY02B10_26 (220 aa), FASTA scores: opt: 134, E(): 0.063, (25.8% identity in 159 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved transmembrane protein 237895 886803 Rv0200 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_214714.1 237206 D 83332 CDS NP_214715.1 15607342 886756 complement(237892..238395) 1 NC_000962.3 Rv0201c, (MTV033.09c), len: 167 aa. Conserved protein, equivalent to Z95398|MLCL622.15c from Mycobacterium leprae (170 aa), FASTA scores: opt: 646, E(): 0, (63.9% identity in 158 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 238395 886756 Rv0201c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214715.1 237892 R 83332 CDS NP_214716.1 15607343 886750 complement(238392..241292) 1 NC_000962.3 Rv0202c, (MTV033.10c), len: 966 aa. Probable mmpL11,conserved transmembrane transport protein (see citation below), equivalent to Z95398|MLCL622.16c from Mycobacterium leprae (1014 aa), FASTA scores: opt: 4076, E(): 0, (72.8% identity in 1017 aa overlap). Member of RND superfamily,similar to several putative transport proteins e.g. P96687 from Bacillus subtilis (724 aa), FASTA scores: opt: 594,E(): 9.1e-29, (26.9% identity in 717 aa overlap); etc. Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL11 241292 mmpL11 886750 mmpL11 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL11 NP_214716.1 238392 R 83332 CDS NP_214717.1 15607344 886748 241514..241924 1 NC_000962.3 Rv0203, (MTV033.11), len: 136 aa. Possible exported protein (has hydrophobic stretch near N-terminus). Some similarity to part of U02459|LDU02459_1 hypothetical protein from Leishmania donovani (741 aa), FASTA score: opt: 111, E(): 9.1, (30.0% identity in 90 aa overlap).; Possible exported protein 241924 886748 Rv0203 Mycobacterium tuberculosis H37Rv Possible exported protein NP_214717.1 241514 D 83332 CDS NP_214718.2 448824741 886747 complement(241976..243214) 1 NC_000962.3 Rv0204c, (MTV033.12c), len: 412 aa. Probable conserved transmembrane protein (see citation below),equivalent, but has C-terminal extension, to Z95398|MLCL622.17c from Mycobacterium leprae (367 aa),FASTA scores: opt: 2002, E(): 0, (82.4% identity in 374 aa overlap). Some similarity to Rv0585c from Mycobacterium tuberculosis. Nucleotide position 242299 in the genome sequence has been corrected, C:G resulting in V306L.; Probable conserved transmembrane protein 243214 886747 Rv0204c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214718.2 241976 R 83332 CDS NP_214719.1 15607346 886766 243384..244487 1 NC_000962.3 Rv0205, (MTV033.13), len: 367 aa. Possible conserved transmembrane protein, similar to hypothetical proteins from many bacteria e.g. AL0209|SC4H8_6 from Streptomyces coelicolor (402 aa), FASTA scores: opt: 436, E(): 1.7e-21,(27.2% identity in 349 aa overlap); Z99117|BSUB0014_221 from Bacillus subtilis (353 aa), FASTA scores: opt: 394,E(): 8.6e-19, (28.7% identity in 324 aa overla).; Probable conserved transmembrane protein 244487 886766 Rv0205 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214719.1 243384 D 83332 CDS NP_214720.1 15607347 886752 complement(244484..247318) 1 NC_000962.3 Rv0206c, (MTV033.14c, MTCY08D5.01c), len: 944 aa. Possible mmpL3, conserved transmembrane transport protein (see Tekaia et al., 1999), equivalent to Z95398|MLCL622.18c from Mycobacterium leprae (955 aa), FASTA scores: opt: 806,E(): 1.8e-21, (57.2% identity in 243 aa overlap). Member of RND superfamily, similar to others. Belongs to the MmpL family.; Possible conserved transmembrane transport protein MmpL3 247318 mmpL3 886752 mmpL3 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane transport protein MmpL3 NP_214720.1 244484 R 83332 CDS NP_214721.1 15607348 886742 complement(247384..248112) 1 NC_000962.3 Rv0207c, (MTCY08D5.02c), len: 242 aa. Conserved hypothetical protein, equivalent to Z95398|MLCL622_19 from Mycobacterium leprae (261 aa), FASTA scores: E(): 0, (60.8 identity in 199 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 248112 886742 Rv0207c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214721.1 247384 R 83332 CDS NP_214722.1 15607349 886740 complement(248115..248906) 1 NC_000962.3 Rv0208c, (MTCY08D5.03c), len: 263 aa. Hypothetical methyltransferase, equivalent to Z95398|MLCL622_20 from Mycobacterium leprae (279 aa), FASTA score: (64.2% identity in 246 aa overlaps). Also similar to others e.g. 10178368|CAC08407.1|AL392177|Q9F305|MT04_STRCO|SCD17A.03c hypothetical methlytransferase from Streptomyces coelicolor (271 aa). Could start at aa 7.; Hypothetical methlytransferase (methylase) 248906 886740 Rv0208c Mycobacterium tuberculosis H37Rv Hypothetical methlytransferase (methylase) NP_214722.1 248115 R 83332 CDS NP_214723.1 15607350 886739 249038..250123 1 NC_000962.3 Rv0209, (MTCY08D5.04), len: 361 aa. Hypothetical unknown protein.; Hypothetical protein 250123 886739 Rv0209 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214723.1 249038 D 83332 CDS NP_214724.1 15607351 886735 250120..251598 1 NC_000962.3 Rv0210, (MTCY08D5.05), len: 492 aa. Hypothetical unknown protein. Possibly membrane protein; has hydrophobic stretches around aa 333 - 381.; Hypothetical protein 251598 886735 Rv0210 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214724.1 250120 D 83332 CDS NP_214725.1 15607352 886744 251782..253602 1 NC_000962.3 Rv0211, (MTCY08D5.06), len: 606 aa. Probable pckA (alternate gene names: pckG and pck1), iron-regulated phosphoenolpyruvate carboxykinase [GTP], equivalent to Z95398|MLCL622_21 probable phosphoenolpyruvate carboxykinase from Mycobacterium leprae (609 aa), FASTA score: (86.1% identity in 605 aa overlap). Also highly similar to others e.g. PPCK_NEOFR|P22130 phosphoenolpyruvate carboxykinase [GTP] (608 aa), FASTA scores: opt: 2287, E(): 0, (55.9% identity in 598 aa overlap). Contains PS00505 Phosphoenolpyruvate carboxykinase (GTP) signature. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.; Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase) 253602 pckA 886744 pckA Mycobacterium tuberculosis H37Rv Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase) NP_214725.1 251782 D 83332 CDS NP_214726.1 15607353 886734 complement(253669..254640) 1 NC_000962.3 Rv0212c, (MTCY08D5.07c), len: 323 aa. Possible nadR (alternate gene name: nadI), transcriptional regulator,similar to others e.g. NADR_ECOLI|P27278 transcriptional regulator from Escherichia coli (410 aa), FASTA scores: opt: 377, E (): 1e-17, (31.1% identity in 347 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible transcriptional regulatory protein NadR (probably AsnC-family) 254640 nadR 886734 nadR Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NadR (probably AsnC-family) NP_214726.1 253669 R 83332 CDS NP_214727.1 15607354 886746 complement(254637..255950) 1 NC_000962.3 Rv0213c, (MTCY08D5.08c), len: 437 aa. Possible methyltransferase, weakly similar to others methyltransferases e.g. AF127374_30|LINA from Streptomyces lavendulae (611 aa), FASTA scores: opt: 400, E(): 8.1e-19,(27.3% identity in 388 aa overlap); Q50258 fortimicin kl1 methyltransferase (553 aa), FASTA scores: opt: 267, E(): 1.2e-13, (29.3% identity in 351 aa overlap).; Possible methyltransferase (methylase) 255950 886746 Rv0213c Mycobacterium tuberculosis H37Rv Possible methyltransferase (methylase) NP_214727.1 254637 R 83332 CDS NP_214728.1 15607355 886737 256064..257677 1 NC_000962.3 Rv0214, (MTCY08D5.09), len: 537 aa. Probable fadD4,fatty-acid-CoA synthetase, similar to many e.g. 4CL_PINTA|P41636 4-coumarate--CoA ligase (537 aa), FASTA scores: opt: 622, E(): 1e-31, (30.0% identity in 514 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY6A4.14 FASTA score: (30.7% identity in 501 aa overlap); MTCY493_27, MTCY07A7_11, MTCI28_6. Contains PS00455 putative AMP-binding domain signature.; Probable fatty-acid-CoA ligase FadD4 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 257677 fadD4 886737 fadD4 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD4 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_214728.1 256064 D 83332 CDS NP_214729.1 15607356 886730 complement(257783..258856) 1 NC_000962.3 Rv0215c, (MTCY08D5.10c), len: 357 aa. Probable fadE3, acyl- dehydrogenase, similar to many e.g. ACDB_BACSU|P45857 acyl-CoA dehydrogenase from B. subtilis (379 aa), FASTA scores: opt: 812, E(): 0, (39.5% identity in 354 aa overlap).; Probable acyl-CoA dehydrogenase FadE3 258856 fadE3 886730 fadE3 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE3 NP_214729.1 257783 R 83332 CDS NP_214730.1 15607357 886729 258913..259926 1 NC_000962.3 Rv0216, (MTCY08D5.11), len: 337 aa. Double hotdog R-specific hydratase of unknown function, shows no activity for crotonyl-CoA, equivalent to Z95398|MLCL622_22 from Mycobacterium leprae (339 aa), FASTA scores: E(): 0, (73.7 identity in 338 aa overlap). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Double hotdog hydratase 259926 886729 Rv0216 Mycobacterium tuberculosis H37Rv Double hotdog hydratase NP_214730.1 258913 D 83332 CDS NP_214731.1 15607358 886726 complement(259923..260831) 1 NC_000962.3 Rv0217c, (MTCY08D5.12c), len: 302 aa. Possible esterase, showing similarity with others e.g. EST_ACICA|P18773 esterase (303 aa), FASTA scores: opt: 320,E(): 3.2e-13, (29.2% identity in 274 aa overlap).; Possible esterase LipW 260831 lipW 886726 lipW Mycobacterium tuberculosis H37Rv Possible esterase LipW NP_214731.1 259923 R 83332 CDS NP_214732.1 15607359 886727 260924..262252 1 NC_000962.3 Rv0218, (MTCY08D5.13), len: 442 aa. Probable conserved transmembrane protein, some similarity with sulfite oxidases e.g. SUOX_HUMAN|P51687 sulfite oxidase precursor (488 aa), FASTA scores: opt: 153, E(): 0.0087,(28.6% identity in 161 aa overlap); and with some nitrate reductases e.g. NIA_FUSOX|P39863 nitrate reductase (NADPH) (905 aa), FASTA scores: opt: 143, E(): 0.06, (29.3% identity in 92 aa overlap). Also similar to BSUB0017_86 from Mycobacterium tuberculosis.; Probable conserved transmembrane protein 262252 886727 Rv0218 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214732.1 260924 D 83332 CDS NP_214733.1 15607360 886725 262254..262802 1 NC_000962.3 Rv0219, (MTCY08D5.14), len: 182 aa. Probable conserved transmembrane protein, showing similarity with CAB76992.1|AL159178 putative lipoprotein from Streptomyces coelicolor (163 aa).; Probable conserved transmembrane protein 262802 886725 Rv0219 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214733.1 262254 D 83332 CDS NP_214734.1 15607361 886722 262812..264023 1 NC_000962.3 Rv0220, (MTCY08D5.15), len: 403 aa. Probable esterase, similar to others proteins and esterases from various organisms and Mycobacterium tuberculosis e.g. Q50681 (431 aa), FASTA scores: opt: 841, E(): 0, (38.2% identity in 408 aa overlap); Rv1426c, Rv1399c, etc. Contains PS00122 Carboxylesterases type-B serine active site.; Probable esterase LipC 264023 lipC 886722 lipC Mycobacterium tuberculosis H37Rv Probable esterase LipC NP_214734.1 262812 D 83332 CDS NP_214735.1 15607362 886719 264067..265476 1 NC_000962.3 Rv0221, (MTCY08D5.16), len: 469 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to other proteins from Mycobacterium tuberculosis e.g. Q50680|Rv2285|MT2343|MTCY339.25c 47.7 kDa protein (445 aa),FASTA scores: opt: 455, E(): 8.1e-23, (26.7% identity in 461 aa overlap); Rv3740c, Rv3734c, etc.; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 265476 886719 Rv0221 Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_214735.1 264067 D 83332 CDS NP_214736.1 15607363 886723 265507..266295 1 NC_000962.3 Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1,enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc.; Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 266295 echA1 886723 echA1 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_214736.1 265507 D 83332 CDS NP_214737.1 15607364 886718 complement(266301..267764) 1 NC_000962.3 Rv0223c, (MTCY08D5.18), len: 487 aa. Probable aldehyde dehydrogenase, similar to others e.g. A75608|6460525|AAF12231.1|AE001862_57 aldehyde dehydrogenase from Deinococcus radiodurans strain R1 (495 aa); Q47943 L-sorbosone dehydrogenase NAD(P) dependent from Gluconobacter oxydans (498 aa), FASTA scores: opt: 1157, E (): 0, (42.1% identity in 482 aa overlap); etc. Also similar to Rv0768, Rv2858c, etc from Mycobacterium tuberculosis. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site; and PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family.; Probable aldehyde dehydrogenase 267764 886718 Rv0223c Mycobacterium tuberculosis H37Rv Probable aldehyde dehydrogenase NP_214737.1 266301 R 83332 CDS NP_214738.1 15607365 886715 complement(267863..268627) 1 NC_000962.3 Rv0224c, (MTCY08D5.19c), len: 254 aa. Possible methyltransferase, showing weak similarity with other methyltransferases e.g. P74388 sterol-C-methyltransferase (318 aa), FASTA scores: opt: 190, E(): 3.6e-05, (33.3% identity in 114 aa overlap). Equivalent to AL022486|MLCB1883_1 from Mycobacterium leprae (269 aa),FASTA scores: opt: 1456, E(): 0, (82.9% identity in 252 aa overlap). Also some similarity with MTCY21B4.22c from Mycobacterium tuberculosis FASTA score: (30.1% identity in 136 aa overlap).; Possible methyltransferase (methylase) 268627 886715 Rv0224c Mycobacterium tuberculosis H37Rv Possible methyltransferase (methylase) NP_214738.1 267863 R 83332 CDS NP_214739.1 15607366 886713 268663..269817 1 NC_000962.3 Rv0225, (MTCY08D5.20), len: 384 aa. Possible conserved protein involved in LPS biosynthesis, similar to O26275 LPS biosynthesis RFBU related protein (382 aa),FASTA scores: opt: 426, E(): 1.2e-20, (28.2% identity in 394 aa overlap). Some similarity with Rv3032 from Mycobacterium tuberculosis FASTA score: (31.6% identity in 228 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved protein 269817 886713 Rv0225 Mycobacterium tuberculosis H37Rv Possible conserved protein NP_214739.1 268663 D 83332 CDS NP_214740.1 15607367 886711 complement(269834..271564) 1 NC_000962.3 Rv0226c, (MTCY08D5.21c), len: 576 aa. Probable conserved transmembrane protein, equivalent, except in N-terminal part, to AC32114.1|AL583926 conserved membrane protein from Mycobacterium leprae (600 aa), FASTA scores: opt: 2086, E(): 0, (70.3% identity in 579 aa overlap). Also similar to AL021411|SC7H1_20 from Streptomyces coelicolor (483 aa), FASTA scores: opt: 180, E(): 0.00028, (26.5 identity in 388 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved transmembrane protein 271564 886711 Rv0226c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214740.1 269834 R 83332 CDS NP_214741.1 15607368 886710 complement(271574..272839) 1 NC_000962.3 Rv0227c, (MTCY08D5.22c), len: 421 aa. Possible conserved membrane protein, equivalent to AL022486|MLCB1883_4 from Mycobacterium leprae (448 aa),FASTA scores: opt: 2148, E(): 0, (76.6% identity in 423 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved membrane protein 272839 886710 Rv0227c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214741.1 271574 R 83332 CDS NP_214742.1 15607369 886708 273055..274278 1 NC_000962.3 Rv0228, (MTCY08D5.23), len: 407 aa. Probable integral membrane acyltransferase, equivalent to 3063875|CAA18555.1|AL022486|T44870 acyltransferase from Mycobacterium leprae (384 aa), FASTA scores: opt: 2004,E(): 0, (79.3% identity in 381 aa overlap). Also similar to others e.g. Q11064 probable acyltransferase CY50.28C (383 aa), FASTA scores: opt: 372, E(): 2.6e-16, (35.9% identity in 359 aa overlap); Q00718|MDMB_STRMY acyltransferase. Very similar to Rv0111, Rv1254, etc from Mycobacterium tuberculosis.; Probable integral membrane acyltransferase 274278 886708 Rv0228 Mycobacterium tuberculosis H37Rv Probable integral membrane acyltransferase NP_214742.1 273055 D 83332 CDS NP_214743.1 15607370 886724 complement(274306..274986) 1 NC_000962.3 Rv0229c, (MTCY08D5.24c), len: 226 aa. Possible conserved membrane protein with PIN domain in C-terminal half, similar to several others from Mycobacterium tuberculosis. Has some similarity with Rv2757c|D70880 from Mycobacterium tuberculosis (138 aa). (See Arcus et al.,2005). FASTA scores: E(): 1e-15, (45.3% identity in 137 aa overlap), and Rv0301, Rv2546, etc. Also some similarity with Q48177 virulence associated protein C (132 aa), FASTA scores: opt: 101, E(): 0.6, (24.3% identity in 136 aa overlap). Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; Possible conserved membrane protein with PIN domain 274986 886724 Rv0229c Mycobacterium tuberculosis H37Rv Possible conserved membrane protein with PIN domain NP_214743.1 274306 R 83332 CDS NP_214744.1 15607371 886705 complement(274983..275963) 1 NC_000962.3 Rv0230c, (MTCY08D5.26c), len: 326 aa. Probable php,phosphotriesterase, similar to others e.g. AAK42653.1|AE006849 putative aryldialkylphosphatase (phosphotriesterase) (paraoxonase) from Sulfolobus solfataricus (314 aa); PHP_ECOLI|P45548 phosphotriesterase homology protein from Escherichia coli (292 aa), FASTA scores: opt: 408, E(): 7.1e-20, (31.1% identity in 305 aa overlap); OPD_FLASP|P16648 parathion hydrolase precursor (365 aa), FASTA scores: opt: 319, E(): 5.1e-14, (34.5% identity in 333 aa overlap); etc. Belongs to the phosphotriesterase family. Cofactor: contains 2 moles of zinc per subunit.; Probable phosphotriesterase Php (parathion hydrolase) (PTE) (aryldialkylphosphatase) (paraoxonase) (a-esterase) (aryltriphosphatase) (paraoxon hydrolase) 275963 php 886705 php Mycobacterium tuberculosis H37Rv Probable phosphotriesterase Php (parathion hydrolase) (PTE) (aryldialkylphosphatase) (paraoxonase) (a-esterase) (aryltriphosphatase) (paraoxon hydrolase) NP_214744.1 274983 R 83332 CDS NP_214745.1 15607372 886703 276058..277764 1 NC_000962.3 Rv0231, (MTCY08D5.27), len: 568 aa. Probable fadE4,acyl-CoA dehydrogenase, similar to many e.g. O29752 acyl-CoA dehydrogenase (ACD-3) from Archaeoglobus fulgidus (576 aa), FASTA scores: opt: 1788, E(): 0, (51.0% identity in 577 aa overlap); ACDB_BACSU|P45857 acyl-CoA dehydrogenase from Bacillus subtilis (379 aa), FASTA scores: opt: 232, E(): 2.2e- 08, (21.6% identity in 291 aa overlap).; Probable acyl-CoA dehydrogenase FadE4 277764 fadE4 886703 fadE4 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE4 NP_214745.1 276058 D 83332 CDS NP_214746.1 15607373 886701 277899..278588 1 NC_000962.3 Rv0232, (MTCY08D5.28), len: 229 aa. Probable transcriptional regulatory protein, TetR/AcrR family,similar to others e.g. YIXD_BACSU|P32398 hypothetical transcriptional regulator (191 aa), FASTA scores: opt: 149,E(): 0.0014, (21.5% identity in 158 aa overlap). Also similar to MTV030_11 from Mycobacterium tuberculosis. Contains PS01081 Bacterial regulatory proteins, TetR family signature, and probable helix-turn helix motif from aa 33-54 (Score 1142, +3.08 SD). Belongs to the TetR/AcrR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably TetR/AcrR-family) 278588 886701 Rv0232 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR/AcrR-family) NP_214746.1 277899 D 83332 CDS NP_214747.1 15607374 886699 278585..279529 1 NC_000962.3 Rv0233, (MTCY08D5.29), len: 314 aa. nrdB (alternate gene name: rnrS) ribonucleoside-diphosphate reductase, beta chain, similar to others e.g. RIR2_SCHPO|P36603 ribonucleoside-diphosphate reductase (391 aa), FASTA scores: opt: 168, E(): 0.00018, (26.1% identity in 199 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: iron, manganese; Ribonucleoside-diphosphate reductase (beta chain) NrdB (ribonucleotide reductase small chain) 279529 nrdB 886699 nrdB Mycobacterium tuberculosis H37Rv Ribonucleoside-diphosphate reductase (beta chain) NrdB (ribonucleotide reductase small chain) NP_214747.1 278585 D 83332 CDS NP_216247.2 57116704 886732 complement(279605..281140) 1 NC_000962.3 Rv0234c, (MTCY08D5.30c), len: 511 aa. gabD1,succinate-semialdehyde dehydrogenase [NADP+] dependent,equivalent to AL022486|MLCB1883_6 probable aldehyde dehydrogenase from Mycobacterium leprae (457 aa), FASTA scores: opt: 2617, E(): 0, (85.7% identity in 455 aa overlap). Also highly similar to Q55585|GABD|SLR0370 probable succinate-semialdehyde dehydrogenase from Synechocystis sp. strain PCC 6803 (454 aa), FASTA scores: opt: 1676, E(): 0, (55.8% identity in 455 aa overlap); and similar to others e.g. GABD_ECOLI|P25526 succinate-semialdehyde dehydrogenase from Escherichia coli (482 aa), FASTA scores: opt: 929, E(): 0, (36.5% identity in 452 aa overlap); etc. Note that similar to other cytosolic aldehyde dehydrogenases with EC number: 1.2.1.3. Also similar to Rv0768|aldA semialdehyde dehydrogenase from Mycobacterium tuberculosis (489 aa); and gabD2|Rv1731|MTCY04C12.16 possible succinate-semialdehyde dehydrogenase [NADP+] dependent from Mycobacterium tuberculosis (518 aa). Contains PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. Could start at different site by homology. Note that previously known as gabD2.; Succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD1 281140 gabD1 886732 gabD1 Mycobacterium tuberculosis H37Rv Succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD1 NP_216247.2 279605 R 83332 CDS NP_214749.1 15607376 886695 complement(281166..282614) 1 NC_000962.3 Rv0235c, (MTCY08D5.31c), len: 482 aa. Probable conserved transmembrane protein, highly similar to AL133278|CAB61913.1|SCM11_2 putative integral membrane protein from Streptomyces coelicolor (470 aa), FASTA scores: opt: 2116, E(): 0, (61.8% identity in 474 aa overlap); and similar to hypothetical proteins from other organisms e.g. Q13392|384D8_7 hypothetical protein (579 aa), FASTA scores: opt: 355, E(): 6.9e-17, (28.5% identity in 569 aa overlap).; Probable conserved transmembrane protein 282614 886695 Rv0235c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214749.1 281166 R 83332 CDS NP_214750.1 15607377 886707 complement(282649..286851) 1 NC_000962.3 Rv0236c, (MTV034.01c, MTV034.02c, MTCY08D5.32c),len: 1400 aa. Possible aftD, arabinofuranosyltransferase (See Skovierova et al., 2009). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian,2003). Probable conserved transmembrane protein, equivalent to AL022486|CAC32102.1|MLCB1883_7 possible integral membrane protein from Mycobacterium leprae (1440 aa), FASTA scores: opt: 7491, E(): 0, (78.8% identity in 1397 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible arabinofuranosyltransferase AftD 286851 aftD 886707 aftD Mycobacterium tuberculosis H37Rv Possible arabinofuranosyltransferase AftD NP_214750.1 282649 R 83332 CDS YP_177619.1 57116705 3205106 complement(286898..287071) 1 NC_000962.3 Rv0236A, len: 57 aa. Small secreted protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Small secreted protein 287071 3205106 Rv0236A Mycobacterium tuberculosis H37Rv Small secreted protein YP_177619.1 286898 R 83332 CDS YP_177702.1 57116706 886693 287186..288352 1 NC_000962.3 Rv0237, (MTV034.03), len: 388 aa. Probable lpQI,conserved lipoprotein, equivalent to AL022486|MLCB1883_8|T44873 probable secreted hydrolase from Mycobacterium leprae (387 aa), FASTA scores: opt: 1831,E(): 0, (73.3% identity in 390 aa overlap). Also similar to other lipoproteins and various hydrolases e.g. P40406|2126897|YBBD_BACSU|I39839 hypothetical 70.6 KDA lipoprotein from Bacillus subtilis (642 aa); P48823|HEXA_ALTSO beta-hexosaminidase a precursor from alteromonas SP. (598 aa), FASTA scores: opt: 415, E(): 5.8e-17, (31.2% identity in 343 aa overlap); PCC6803|P74340 beta-glucosidase from Synechocystis sp. (538 aa), FASTA scores: opt: 414, E(): 6.1e-17, (30.6 identity in 320 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LpqI 288352 lpqI 886693 lpqI Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqI YP_177702.1 287186 D 83332 CDS NP_214752.1 15607379 886691 288428..289042 1 NC_000962.3 Rv0238, (MTV034.04), len: 204 aa. Possible transcriptional regulatory protein, TetR family, equivalent to AL022486|MLCB1883_9|T44874 probable transcription regulator from Mycobacterium leprae (208 aa), FASTA scores: opt: 1029, E(): 0, (80.9% identity in 199 aa overlap). Also similar to others e.g. CAB77290.1|AL160312 putative TetR-family regulatory protein from Streptomyces coelicolor (240 aa). Also similar to Mycobacterium tuberculosis proteins Z95120|Rv3208 (228 aa), FASTA scores: opt: 266,E(): 8.3e-12, (28.1% identity in 196 aa overlap); and Rv1019 (197 aa).; Possible transcriptional regulatory protein (probably TetR-family) 289042 886691 Rv0238 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably TetR-family) NP_214752.1 288428 D 83332 CDS NP_214753.1 15607380 886689 289104..289337 1 NC_000962.3 Rv0239, (MTV034.05), len: 77 aa. Possible vapB24,antitoxin, part of toxin-antitoxin (TA) operon with Rv0240. Weakly similar to others e.g. Rv1839c|Z83859|MTCY359_34 from Mycobacterium tuberculosis (87 aa). See Arcus et al. 2005. FASTA scores: opt: 88, E(): 5, (40.0% identity in 45 aa overlap).; Possible antitoxin VapB24 289337 vapB24 886689 vapB24 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB24 NP_214753.1 289104 D 83332 CDS NP_214754.1 15607381 886688 289345..289782 1 NC_000962.3 Rv0240, (MTV034.06), len: 145 aa. Possible vapC24,toxin, part of toxin-antitoxin (TA) operon with Rv0239,contains PIN domain, weak similarity with Rv3697c from Mycobacterium tuberculosis (145 aa). See Arcus et al. 2005. FASTA scores: opt: 145, E(): 7.6e-05, (28.0% identity in 143 aa overlap).; Possible toxin VapC24 Contains PIN domain 289782 vapC24 886688 vapC24 Mycobacterium tuberculosis H37Rv Possible toxin VapC24 Contains PIN domain NP_214754.1 289345 D 83332 CDS NP_214755.1 15607382 886686 complement(289812..290654) 1 NC_000962.3 Rv0241c, (MTV034.07c), len: 280 aa. Probable htdX,3-hydroxyacyl-thioester dehydratase (See Gurvitz et al.,2009), highly similar to MLCB1883.17c|T44876063881|CAA18566.1|AL022486 hypothetical protein from Mycobacterium leprae (280 aa), FASTA scores: opt: 1564, E(): 0, (81.8% identity in 280 aa overlap); and CAC32097.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (300 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also similar to proteins from other organisms e.g. CAB77291.1|AL160312 putative dehydratase from Streptomyces coelicolor (291 aa); part of BAA92930.1|AB032743 fatty acid synthetase beta subunit from Pichia angusta (2060 aa). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable 3-hydroxyacyl-thioester dehydratase HtdX 290654 htdX 886686 htdX Mycobacterium tuberculosis H37Rv Probable 3-hydroxyacyl-thioester dehydratase HtdX NP_214755.1 289812 R 83332 CDS NP_214756.1 15607383 886697 complement(290665..292029) 1 NC_000962.3 Rv0242c, (MTV034.08c), len: 454 aa. Probable fabG4,3-oxoacyl-[acyl-carrier protein] reductase, equivalent to 3063883|CAA18568.1|AL022486|MLCB1883_13|T44878 3-oxoacyl-[acyl-carrier protein] reductase homolog from Mycobacterium leprae (454 aa), FASTA scores: opt: 2486,E(): 0, (84.8% identity in 454 aa overlap). C-terminal part highly similar to many FabG proteins e.g. U39441|VHU3944 1_2 from Vibrio harveyi (244 aa), FASTA scores: opt: 562,E(): 3.4e-28, (40.2% identity in 241 aa overlap); U91631|PAU91631_3 from Pseudomonas aeruginosa (247 aa),FASTA scores: opt: 584, E(): 1.5e-29, (44.4% identity in 241 aa overlap). Has N-terminal extension of ~200 aa and C-terminal part contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG4 (3-ketoacyl-acyl carrier protein reductase) 292029 fabG4 886697 fabG4 Mycobacterium tuberculosis H37Rv Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG4 (3-ketoacyl-acyl carrier protein reductase) NP_214756.1 290665 R 83332 CDS NP_214757.1 15607384 886682 292171..293493 1 NC_000962.3 Rv0243, (MTV034.09), len: 440 aa. Probable fadA2,acetyl-CoA acyltransferase (3-acyl-CoA thiolase),equivalent, but shorter 17 aa, to AL022486|MLCB1883_14T44879 acetyltransferase from Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7,E(): 0, (87.6% identity in 435 aa overlap). Also highly similar to many e.g. G83046|PA478 probable acyl-CoA thiolase from Pseudomonas aeruginosa (425 aa); AB77293.1|AL160312 putative ketoacyl CoA thiolase from Streptomyces coelicolor (428 aa); P76503|7449731|YFCY_ECOLI|D65007|B2342 probable 3-ketoacyl-CoA thiolase (acetyl-CoA acyltransferase) (beta-ketothiolase) from Escherichia coli strain K-12 (436 aa), FASTA scores: opt: 914, E(): 0, (38.2% identity in 434 aa overlap); P55084|ECHB_HUMAN mitochondrial trifunctonal enzyme (474 aa), FASTA scores: opt: 881, E(): 0, (37.7 identity in 451 aa overlap). Contains PS00099 Thiolases active site. Belongs to the thiolase family.; Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase) 293493 fadA2 886682 fadA2 Mycobacterium tuberculosis H37Rv Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase) NP_214757.1 292171 D 83332 CDS NP_214758.1 15607385 886698 complement(293798..295633) 1 NC_000962.3 Rv0244c, (MTV034.10c), len: 611 aa. Probable fadE5,acyl-CoA dehydrogenase, equivalent to AL022486|MLCB1883_15 from Mycobacterium leprae (611 aa), FASTA scores: opt: 3598, E(): 0, (89.4% identity in 611 aa overlap). Also highly similar to AL0211|MTV007.14 from Mycobacterium tuberculosis (609 aa), FASTA scores: opt: 2576, E(): 0,(64.6% identity in 611 aa overlap); and to various other bacterial proteins described as putative acyl-CoA dehydrogenases e.g. AE0010|AE001025_6 from Archaeoglobus fulgidus (387 aa), FASTA scores: opt: 229, E(): 6.8e-08,(29.8% identity in 409 aa overlap); etc.; Probable acyl-CoA dehydrogenase FadE5 295633 fadE5 886698 fadE5 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE5 NP_214758.1 293798 R 83332 CDS NP_214759.1 15607386 886680 296005..296493 1 NC_000962.3 Rv0245, (MTV034.11), len: 162 aa. Possible oxidoreductase, equivalent to AL022486|MLCB1883_17|T44882 probable oxidoreductase from Mycobacterium leprae (162 aa),FASTA scores: opt: 860, E(): 0, (83.4% identity in 157 aa overlap). Also similar to several hypothetical proteins and various oxidoreductases e.g. AAK24246.1|AE005898 NADH:riboflavin 5'-phosphate oxidoreductase from Caulobacter crescentus (174 aa); Q02058|DIM6_STRCO|CAA45048.1 actinorhodin polyketide dimerase from streptomyces coelicolor (177 aa), FASTA scores: opt: 308, E(): 3. 2e-15, (37.8% identity in 143 aa overlap). Also similar to Z84498|Rv1939|MTCY09F9.25c from Mycobacterium tuberculosis (171 aa), FASTA scores: opt: 517, E(): 3.5e-30, (49.4% identity in 158 aa overlap).; Possible oxidoreductase 296493 886680 Rv0245 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214759.1 296005 D 83332 CDS NP_214760.1 15607387 886678 296809..298119 1 NC_000962.3 Rv0246, (MTV034.12), len: 436 aa (start uncertain). Probable conserved integral membrane protein, similar to Rv2209|1237062|CAA94252.1|Z70283|Q10398|YM09_MYCTU from Mycobacterium tuberculosis (512 aa), FASTA scores: opt: 712, E(): 0, (33.2% identity in 422 aa overlap).; Probable conserved integral membrane protein 298119 886678 Rv0246 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_214760.1 296809 D 83332 CDS NP_214761.1 15607388 886677 complement(298116..298862) 1 NC_000962.3 Rv0247c, (MTV034.13c), len: 248 aa. Probable succinate dehydrogenase, iron-sulfur subunit, highly similar to CAC44313.1|AL596043 putative succinate dehydrogenase iron-sulfur subunit from Streptomyces coelicolor (259 aa); and similar to iron-sulphur protein subunits of fumarate reductase or succinate dehydrogenases from many bacteria e.g. NP_147618.1|7521083|B72691 fumarate reductase iron-sulfur protein from Aeropyrum pernix (305 aa); NP_069516.1|2649932|AAB90556.1|AE001057 succinate dehydrogenase iron-sulfur subunit B (sdhB) from Archaeoglobus fulgidus (236 aa); etc. Also similar to Q10761|FRDB_MYCTU|7431693|F70762 fumarate reductase iron-sulfur protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 358, E():1e-16, (31.3% identity in 214 aa overlap). Contains PS00197 2Fe-2S ferredoxins,iron-sulfur binding region signature. Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?).; Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) 298862 886677 Rv0247c Mycobacterium tuberculosis H37Rv Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) NP_214761.1 298116 R 83332 CDS NP_214762.1 15607389 886675 complement(298863..300803) 1 NC_000962.3 Rv0248c, (MTV034.14c), len: 646 aa. Probable succinate dehydrogenase, flavoprotein subunit, highly similar to flavoprotein subunit of various succinate dehydrogenases e.g. M88696|RIRSDHA_1 flavoprotein from Rickettsia prowazekii (596 aa), FASTA scores: opt: 651,E(): 0, (34.6 % identity in 598 aa overlap). Also similar to truncated U00022_17 flavoprotein from Mycobacterium leprae (401 aa), FASTA scores: opt: 677, E(): 0, (39.0% identity in 423 aa overlap). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?).; Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) 300803 886675 Rv0248c Mycobacterium tuberculosis H37Rv Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) NP_214762.1 298863 R 83332 CDS NP_214763.1 15607390 886671 complement(300834..301655) 1 NC_000962.3 Rv0249c, (MTV034.15c), len: 273 aa. Probable succinate dehydrogenase, membrane-anchor subunit for succinate dehydrogenase encoded by Rv0247c and Rv0248c. Highly similar to AC44315.1|AL596043 putative integral membrane protein from Streptomyces coelicolor (278 aa). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?).; Probable succinate dehydrogenase [membrane anchor subunit] (succinic dehydrogenase) 301655 886671 Rv0249c Mycobacterium tuberculosis H37Rv Probable succinate dehydrogenase [membrane anchor subunit] (succinic dehydrogenase) NP_214763.1 300834 R 83332 CDS NP_214764.1 15607391 886669 complement(301735..302028) 1 NC_000962.3 Rv0250c, (MTV034.16c), len: 97 aa. Conserved protein, equivalent to MLCB1883.27c|T44883|3063888|CAA18576.1|AL022486 hypothetical protein from Mycobacterium leprae (98 aa),FASTA scores: opt: 478, E(): 4.4e-28, (72.6% identity in 95 aa overlap). Also similar to C-terminus of AC44316.1|AL596043|SCBAC31E11.05c hypothetical protein from Streptomyces coelicolor (146 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 302028 886669 Rv0250c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214764.1 301735 R 83332 CDS NP_214765.1 15607392 886667 complement(302173..302652) 1 NC_000962.3 Rv0251c, (MTV034.17c), len: 159 aa. Hsp (alternate gene name: hsp20, hrpA, acr2), heat-stress-induced ribosome-binding protein A (see citations below). Highly similar to AAD39038.1|AF072875_1|AF072875 putative HSP20 from Mycobacterium smegmatis (145 aa), FASTA scores: opt: 479, E(): 2.3e-24, (59.9% identity in 157 aa overlap); and similar to many bacterial and eukaryotic hsp proteins e.g. P12811|HS2C_CHLRE chloroplast heat shock 22KD protein from chlamydomonas reinhardtii (157 aa), FASTA scores: opt: 184,E(): 1.2e-05, (32.4% identity in 142 aa overlap). Also similar to PCC6803 Spore protein sp21 from Synechocystis sp. (146 aa), FASTA scores: opt: 213, E(): 1.2e-07, (30.3 identity in 145 aa overlap). Also similar to P30223|14KD_MYCTU 14 KDA antigen (16 KDA antigen) 19K major membrane protein (HSP 16.3) from Mycobacterium tuberculosis (144 aa). Belongs to the small heat shock protein (HSP20) family.; Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A) 302652 hsp 886667 hsp Mycobacterium tuberculosis H37Rv Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A) NP_214765.1 302173 R 83332 CDS NP_214766.1 15607393 886665 302866..305427 1 NC_000962.3 Rv0252, (MTV034.18), len: 853 aa. Probable nirB (alternate gene name: nasB), nitrite reductase [NAD(P)H] large subunit, flavoprotein containing siroheme and a 2FE-2S iron-sulfur centre. Highly similar to many others bacterial enzymes e.g. P08201|NIRB_ECOLI nitrite reductase (NAD(P)H) large subunit from Escherichia coli strain K12 (847 aa), FASTA scores: opt: 2775, E(): 0, (49.8% identity in 840 aa overlap); Q06458|NIRB_KLEPN nitrite reductase (NAD(P)H) large subunit (957 aa), FASTA scores: opt: 2902,E(): 0, (54.2% identity in 827 aa overlap). Contains PS00365 Nitrite and sulfite reductases iron-sulfur/siroheme-binding site. Homodimer which associates with NIRD|Rv0253. Cofactors: FAD; Iron; Siroheme.; Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB 305427 nirB 886665 nirB Mycobacterium tuberculosis H37Rv Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB NP_214766.1 302866 D 83332 CDS NP_214767.1 15607394 886664 305453..305809 1 NC_000962.3 Rv0253, (MTV034.19), len: 118 aa. Probable nirD,nitrite reductase [NAD(P)H] small subunit, similar to others e.g. P23675|NIRD_ECOLI|B3366|Z4727|ECS4217 from Escherichia coli strains K12 and O157:H7 (108 aa), FASTA scores: opt: 271, E():1.7e-12, (41.9% identity in 105 aa overlap). Associates with NIRB|Rv0252.; Probable nitrite reductase [NAD(P)H] small subunit NirD 305809 nirD 886664 nirD Mycobacterium tuberculosis H37Rv Probable nitrite reductase [NAD(P)H] small subunit NirD NP_214767.1 305453 D 83332 CDS NP_214768.1 15607395 886661 complement(305825..306349) 1 NC_000962.3 Rv0254c, (MTV034.20), len: 174 aa. Probable cobU,cobalamin biosynthesis protein including a cobinamide kinase and cobinamide phosphate guanylyltransferase. Highly similar to many e.g. Q05599|COBU_SALTY cobinamide kinase / cobinamide phosphate guanylyltransferase from Salmonella typhimurium (181 aa), FASTA scores: opt: 308, E(): 1.1e-14,(38.7% identity in 181 aa overlap); P46886|COBU_ECOLI|B1993|Z3153|ECS2788 Bifunctional cobalamin biosynthesis protein cobU from Escherichia coli strains K12 and O157:H7 (181 aa); part of AL096872|SC5F7_10 from Streptomyces coelicolor (397 aa), FASTA scores: opt: 445, E(): 3.6e-23, (46.0% identity in 176 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable bifunctional cobalamin biosynthesis protein CobU: cobinamide kinase + cobinamide phosphate guanylyltransferase 306349 cobU 886661 cobU Mycobacterium tuberculosis H37Rv Probable bifunctional cobalamin biosynthesis protein CobU: cobinamide kinase + cobinamide phosphate guanylyltransferase NP_214768.1 305825 R 83332 CDS YP_177703.1 57116707 886673 complement(306374..307858) 1 NC_000962.3 Rv0255c, (MTV034.21c), len: 494 aa. Probable cobQ1,cobyric acid synthase, similar to many e.g. Z46611|RCBLUGNS_8 cobyric acid synthase from R.capsulatus (483 aa), FASTA scores: opt: 1239, E(): 0, (47.1% identity in 493 aa overlap); P29932|COBQ_PSEDE cobyric acid synthase from Pseudomonas denitrificans (484 aa), FASTA scores: opt: 1168, E():0, (44.9% identity in 490 aa overlap); etc. Belongs to the COBB/COBQ family, COBQ subfamily. Note that previously known as cobQ.; Probable cobyric acid synthase CobQ1 307858 cobQ1 886673 cobQ1 Mycobacterium tuberculosis H37Rv Probable cobyric acid synthase CobQ1 YP_177703.1 306374 R 83332 CDS YP_177704.1 57116708 886684 complement(307877..309547) 1 NC_000962.3 Rv0256c, (MTV034.22c), len: 556 aa. PPE2, Member of the M. tuberculosis PPE family, similar to many e.g. Rv0280, Rv0286, etc. Equivalent to Z98756|MLCB2492.30 from Mycobacterium leprae (572 aa), FASTA scores: opt: 1837,E(): 0, (62.9% identity in 461 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; PPE family protein PPE2 309547 PPE2 886684 PPE2 Mycobacterium tuberculosis H37Rv PPE family protein PPE2 YP_177704.1 307877 R 83332 CDS YP_177620.1 57116709 3205110 309699..310073 1 NC_000962.3 Rv0257, len: 124 aa. Hypothetical protein,orthologue of ML1828A conserved hypothetical protein from Mycobacterium leprae. Replaced Rv0257c (older annotation). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 310073 3205110 Rv0257 Mycobacterium tuberculosis H37Rv hypothetical protein YP_177620.1 309699 D 83332 CDS NP_214772.1 15607399 886654 complement(310294..310749) 1 NC_000962.3 Rv0258c, (MTCY06A4.02c), len: 151 aa (alternative start possible). Conserved hypothetical protein, showing some similarity to Rv1685c|MTCI125_6 from Mycobacterium tuberculosis (207 aa), FASTA scores: E(): 9.3e-07, (32.1% identity in 140 aa overlap). Also some similarity with AL049819|SCE7_13|T36295 probable transcription regulator from Streptomyces coelicolor (204 aa), FASTA scores: opt: 158, E(): 0.00052, (27.0% identity in 111 aa overlap).; hypothetical protein 310749 886654 Rv0258c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214772.1 310294 R 83332 CDS NP_214773.1 15607400 886657 complement(310774..311517) 1 NC_000962.3 Rv0259c, (MTCY06A4.03c), len: 247 aa. Conserved hypothetical protein, showing some similarity to Rv2393|Z81368|MTCY253_28 from Mycobacterium tuberculosis (281 aa), FASTA scores: E(): 9.5e-16, (33.6 % identity in 235 aa overlap). Also some similarity with CAC33938.1|AL589708 putative secreted protein from Streptomyces coelicolor (248 aa).; hypothetical protein 311517 886657 Rv0259c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214773.1 310774 R 83332 CDS NP_214774.1 15607401 886651 complement(311514..312659) 1 NC_000962.3 Rv0260c, (MTCY0A4.04c), len: 381 aa. Possible two-component response regulator, highly similar to CAB72204.1|AL138851 putative transcriptional regulator from Streptomyces coelicolor (395 aa); and similar to O34394|D69851|YJJA conserved hypothetical protein from Bacillus subtilis (270 aa), FASTA scores: opt: 312, E(): 7.4e-14, (25.8% identity in 267 aa overlap). Also some similarity to regulatory proteins at C-terminal region e.g. CUTR_STRLI|Q03756 transcriptional regulatory protein (217 aa), FASTA scores: opt: 138, E(): 0.02, (30.6% identity in 111 aa overlap).; Possible transcriptional regulatory protein 312659 886651 Rv0260c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214774.1 311514 R 83332 CDS NP_214775.1 15607402 886663 complement(312759..314168) 1 NC_000962.3 Rv0261c, (MTCY06A4.05c), len: 469 aa. Probable nirK3, nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS),equivalent to AAB41700.1|U72744 nitrite extrusion protein from Mycobacterium fortuitum (471 aa); and 2342627|CAB11406.1|Z98741|T44908 nitrite extrusion protein homolog from Mycobacterium leprae (517 aa; longer in N-terminus). Also similar to other nitrite extrusion proteins e.g. NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 755, E(): 0, (35.0% identity in 466 aa overlap). Belongs to the nark/NASA family of transporters.; Probable integral membrane nitrite extrusion protein NarK3 (nitrite facilitator) 314168 narK3 886663 narK3 Mycobacterium tuberculosis H37Rv Probable integral membrane nitrite extrusion protein NarK3 (nitrite facilitator) NP_214775.1 312759 R 83332 CDS NP_214776.1 15607403 886648 complement(314309..314854) 1 NC_000962.3 Rv0262c, (MTCY06A4.06c), len: 181 aa. Aac,aminoglycoside 2'-N-acetyltransferase (aac(2')-IC) (see citation below), highly similar to NP_302635.1|NC_002677 aminoglycoside 2'-N-acetyltransferase from Mycobacterium leprae (182 aa); Q49157|AAC2_MYCFO|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium fortuitum (195 aa), Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. FASTA scores: opt: 884,E(): 0, (69.1% identity in 181 aa overlap); and P94968|AAC2_MYCSM|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium smegmatis (210 aa) (see also citation below). Also similar to Q52424|AAC2_PROST aminoglycoside 2'-N-acetyltransferase from Providencia stuartii (178 aa). Belongs to the AAC(2')-I family of acetyltransferases. Note that previously known as aac(2')-IC.; Aminoglycoside 2'-N-acetyltransferase Aac (Aac(2')-IC) 314854 aac 886648 aac Mycobacterium tuberculosis H37Rv Aminoglycoside 2'-N-acetyltransferase Aac (Aac(2')-IC) NP_214776.1 314309 R 83332 CDS NP_214777.1 15607404 886659 complement(314864..315766) 1 NC_000962.3 Rv0263c, (MTCY06A4.07c), len: 300 aa. Conserved hypothetical protein, equivalent to NP_302634.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (305 aa). Also similar to others e.g. AL121596|SC51A_21 hypothetical protein from Streptomyces coelicolor (285 aa),FASTA scores: opt: 714, E(): 0, (45.3% identity in 289 aa overlap); NP_233164.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (309 aa); NP_406216.1|NC_003143 conserved hypothetical protein from Yersinia pestis (316 aa); YH30_HAEIN|P44298|hi1730 hypothetical protein from Haemophilus influenzae (309 aa),FASTA scores: opt: 430, E(): 3e-20, (29.6% identity in 284 aa overlap); etc. Also similar to carboxylases eg NP_415240.1|NC_000913|P75745|YBGK_ECOLI putative carboxylase from Escherichia coli strain K12 (310 aa),FASTA score: (34.6% identity in 286 aa overlap); NP_459698.1|NC_003197 putative carboxylase from Salmonella typhimurium (310 aa); and to middle part of NP_420636.1|NC_002696 urea amidolyase-related protein from Caulobacter crescentus (1207 aa).; hypothetical protein 315766 886659 Rv0263c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214777.1 314864 R 83332 CDS NP_214778.1 15607405 886646 complement(315783..316415) 1 NC_000962.3 Rv0264c, (MTCY06A4.08c), len: 210 aa. Conserved hypothetical protein, equivalent to CAC32080.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (222 aa). Also similar to others hypothetical proteins e.g. AL121596|SC51A_20 from Streptomyces coelicolor (252 aa),FASTA scores: opt: 420, E(): 2.7e-20, (41.7% identity in 204 aa overlap); P75744|YBGJ_ECOLI hypothetical 23.9 KD protein from Escherichia coli (218 aa), FASTA scores: E(): 2.1e-14, (35.7% identity in 182 aa overlap); YH31_HAEIN|P44299|hi173 hypothetical protein from Haemophilus influenzae (213 aa), FASTA scores: opt: 252,E(): 8.3e-10, (31.1% identity in 183 aa overlap).; hypothetical protein 316415 886646 Rv0264c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214778.1 315783 R 83332 CDS YP_177705.1 57116710 886650 complement(316511..317503) 1 NC_000962.3 Rv0265c, (MTCY06A4.09c), len: 330 aa. Probable iron-transport lipoprotein, most similar to T36412|5763945|CAB53324.1|AL109974 probable iron-siderophore binding lipoprotein from Streptomyces coelicolor (350 aa); and (N-terminus may be incorrect) to T14166|3560508|AAC82551.1|AF027770 fxuD protein from Mycobacterium smegmatis (420 aa), FASTA scores: opt: 385,E(): 1.5e-16, (32.3% identity in 232 aa overlap). Also similar to AAB97475.1|U02617 DtxR/iron regulated lipoprotein precursor from Corynebacterium diphtheriae (355 aa); FECB_ECOLI|P15028 iron(III) dicitrate-binding periplasmic protein (300 aa), FASTA scores: opt: 191, E(): 2.3e-05, (26.5% identity in 196 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as fecB2.; Probable periplasmic iron-transport lipoprotein 317503 886650 Rv0265c Mycobacterium tuberculosis H37Rv Probable periplasmic iron-transport lipoprotein YP_177705.1 316511 R 83332 CDS NP_214780.1 15607407 886642 complement(317525..321154) 1 NC_000962.3 Rv0266c, (MTCY06A4.10c), len: 1209 aa. Probable oplA, 5-oxoprolinase, highly similar to others or to hypothetical proteins e.g. AAK24340.1|AE005906 hydantoinase/oxoprolinase from Caulobacter crescentus (1196 aa); NP_103129.1|14022305|BAB48915.1|AP002997 5-oxoprolinase from Mesorhizobium loti (1210 aa); CAC48426.1|AL603642 conserved hypothetical protein from Sinorhizobium meliloti (1205 aa); S77037|slr0697|1006579|BAA10729.1|D6400 hypothetical protein from Synechocystis sp. strain PCC 6803 (1252 aa),FASTA scores: opt: 2016, E(): 0, (51.4% identity in 1247 aa overlap); P97608|OPLA_RAT|T42756|11278797 5-oxoprolinase (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) from Rattus norvegicus (1288 aa); etc. Belongs to the oxoprolinase family.; Probable 5-oxoprolinase OplA (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) 321154 oplA 886642 oplA Mycobacterium tuberculosis H37Rv Probable 5-oxoprolinase OplA (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) NP_214780.1 317525 R 83332 CDS NP_214781.1 15607408 886644 321331..322722 1 NC_000962.3 Rv0267, (MTCY06A4.11), len: 463 aa. Probable narU,nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS),similar to other nitrite extrusion proteins e.g. NARU_ECOLI|P37758 nitrite extrusion protein 2 from Escherichia coli (462 aa), FASTA scores: opt: 630, E(): 4.4e-33, (38.9% identity in 463 aa overlap); and NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 607, E(): 1.3e-31, (42.0% identity in 457 aa overlap). Also similar to Rv0261c, Rv2329c, Rv1737c, and to MLCB22_25 from Mycobacterium leprae (517 aa), FASTA score: (35.1 identity in 459 aa overlap). Belongs to the nark/NASA family of transporters.; Probable integral membrane nitrite extrusion protein NarU (nitrite facilitator) 322722 narU 886644 narU Mycobacterium tuberculosis H37Rv Probable integral membrane nitrite extrusion protein NarU (nitrite facilitator) NP_214781.1 321331 D 83332 CDS NP_214782.1 15607409 886647 complement(322764..323273) 1 NC_000962.3 Rv0268c, (MTCY06A4.12c), len: 169 aa. Hypothetical unknown protein.; Hypothetical protein 323273 886647 Rv0268c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214782.1 322764 R 83332 CDS NP_214783.1 15607410 886640 complement(323338..324531) 1 NC_000962.3 Rv0269c, (MTCY06A4.13c), len: 397 aa. Conserved hypothetical protein, highly similar to AL079355|SC4C6_19 hypothetical protein from Streptomyces coelicolor (341 aa),FASTA scores: opt: 1019, E(): 0, (46.5% identity in 344 aa overlap), and similar to other proteins e.g. CAC49016.1|AL603644 putative ATP-dependent DNA ligase protein from Sinorhizobium meliloti (636 aa); O34398 YKOU protein from Bacillus subtilis (611 aa), FASTA score: (27.2% identity in 283 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3062,Rv3731 (both DNA ligases), and Rv0938, Rv3730c.; hypothetical protein 324531 886640 Rv0269c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214783.1 323338 R 83332 CDS NP_214784.1 15607411 886637 324567..326249 1 NC_000962.3 Rv0270, (MTCY06A4.14), len: 560 aa. Probable fadD2,fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 544, E(): 2.9e-26, (27.7% identity in 535 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY493_2,MTCY8D5_9, MTCY6G11_8, etc. Contains PS00455 Putative AMP-binding domain signature.; Probable fatty-acid-CoA ligase FadD2 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 326249 fadD2 886637 fadD2 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD2 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_214784.1 324567 D 83332 CDS NP_214785.1 15607412 886641 complement(326266..328461) 1 NC_000962.3 Rv0271c, (MTCY06A4.15c), len: 731 aa. Probable fadE6, acyl-CoA dehydrogenase, with C-terminal half similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase (short-chain) from Homo sapiens (412 aa), FASTA scores: opt: 339, E(): 1.3e-13, (28.1% identity in 288 aa overlap).; Probable acyl-CoA dehydrogenase FadE6 328461 fadE6 886641 fadE6 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE6 NP_214785.1 326266 R 83332 CDS NP_214786.1 15607413 886635 complement(328575..329708) 1 NC_000962.3 Rv0272c, (MTCY06A4.16c), len: 377 aa. Unknown protein.; hypothetical protein 329708 886635 Rv0272c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214786.1 328575 R 83332 CDS NP_214787.1 15607414 886633 complement(329705..330325) 1 NC_000962.3 Rv0273c, (MTV035.01c), len: 206 aa (start uncertain). Possible transcriptional regulator, showing some similarity to hypothetical regulators from Mycobacterium tuberculosis e.g. P96222|Rv3855|MTCY01A6.13c (216 aa); O08377|Rv1534|MTCY07A7A.03 (225 aa), FASTA scores: opt: 123, E(): 3.2e-06, (28.5% identity in 172 aa overlap).; Possible transcriptional regulatory protein 330325 886633 Rv0273c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214787.1 329705 R 83332 CDS NP_214788.1 15607415 886631 330422..331003 1 NC_000962.3 Rv0274, (MTV035.02), len: 193 aa. Conserved protein,highly similar to AAK25058.1|AE005973 conserved hypothetical protein from Caulobacter crescentus (174 aa). Shows also some similarity to others hypothetical proteins e.g. AJ002571|BSAJ2571_7 from Bacillus subtilis (316 aa),FASTA scores: opt: 138, E(): 0.033, (27.1% identity in 133 aa overlap). Previous hits with Q56415|M85195 fosfomycin-resistance protein from serratia marcescens (141 aa), FASTA scores: opt: 82, E(): 1.1e -08, (29.1% identity in 151 aa overlap). Contains PS00082 Extradiol ring-cleavage dioxygenases signature near C-terminus. May belong to the vicinal-oxygen-chelate (VOC) superfamily of metalloenzymes (See Rawat et al., 2003).; hypothetical protein 331003 886631 Rv0274 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214788.1 330422 D 83332 CDS YP_177706.1 57116711 886629 complement(330933..331658) 1 NC_000962.3 Rv0275c, (MTV035.03c), len: 241 aa. Possible transcriptional regulator, TetR family, similar to others e.g. Q9RJE7|SCF81.04c putative TetR-family transcriptional regulator from Streptomyces coelicolor (219 aa); Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa); Q9I2Q9|PA1836 probable transcriptional regulator from Pseudomonas aeruginosa (193 aa); etc. Also shows some similarity with Rv0825c from Mycobacterium tuberculosis (213 aa), FASTA scores: opt: 230, E(): 2.7e-07, (32.6% identity in 190 aa overlap). Seems to belong to the TetR/AcrR family of transcriptional regulators (M. tuberculosis regulatory protein family with many TetR orthologues).; Possible transcriptional regulatory protein (possibly TetR-family) 331658 886629 Rv0275c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (possibly TetR-family) YP_177706.1 330933 R 83332 CDS NP_214790.1 15607417 886627 331748..332668 1 NC_000962.3 Rv0276, (MTV035.04), len: 306 aa. Conserved hypothetical protein, similar to Rv2237|Z70692|MTCY427.18 from Mycobacterium tuberculosis (296 aa), FASTA scores: opt: 874, E(): 0, (49.6% identity in 282 aa overlap).; hypothetical protein 332668 886627 Rv0276 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214790.1 331748 D 83332 CDS NP_214791.1 15607418 886625 complement(332708..333136) 1 NC_000962.3 Rv0277c, (MTV035.05c), len: 142 aa. Possible vapC25,toxin, part of toxin-antitoxin (TA) operon with Rv0277A,contains PIN domain, see Arcus et al. 2005. Highly similar to others e.g. Rv0749|H70824|2911023|CAA17516.1|AL021958 conserved hypothetical protein from Mycobacterium tuberculosis (142 aa); and Rv2530c, etc.; Possible toxin VapC25 Contains PIN domain 333136 vapC25 886625 vapC25 Mycobacterium tuberculosis H37Rv Possible toxin VapC25 Contains PIN domain NP_214791.1 332708 R 83332 CDS YP_177707.1 57116712 886623 complement(333437..336310) 1 NC_000962.3 Rv0278c, (MTV035.06c), len: 957 aa. PE_PGRS3, Member of the Mycobacterium tuberculosis PE family (see citation below), PGRS subfamily of gly-rich proteins, similar to many e.g. Z95890|MTCY28_25|Rv1759c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 3849, E(): 0,(67.8% identity in 903 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1.; PE-PGRS family protein PE_PGRS3 336310 PE_PGRS3 886623 PE_PGRS3 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS3 YP_177707.1 333437 R 83332 CDS YP_177708.1 57116713 886621 complement(336560..339073) 1 NC_000962.3 Rv0279c, (MTV035.07c), len: 837 aa. PE_PGRS4, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002),similar to many e.g. Z95890|MTCY28_25|Rv0278c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 2677, E(): 0, (64.5% identity in 926 aa overlap).; PE-PGRS family protein PE_PGRS4 339073 PE_PGRS4 886621 PE_PGRS4 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS4 YP_177708.1 336560 R 83332 CDS YP_177709.1 57116714 886619 339364..340974 1 NC_000962.3 Rv0280, (MTV035.08), len: 536 aa. PPE3, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. Z80108|MTCY21B4_4|Rv0453 from Mycobacterium tuberculosis (539 aa), FASTA scores: opt: 1131, E(): 0,(51.7% identity in 540 aa overlap).; PPE family protein PPE3 340974 PPE3 886619 PPE3 Mycobacterium tuberculosis H37Rv PPE family protein PPE3 YP_177709.1 339364 D 83332 CDS NP_214795.1 15607422 886618 340998..341906 1 NC_000962.3 Rv0281, (MTV035.09), len: 302 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family that includes Rv0726c, Rv0731c, Rv3399, Rv1729c,etc. MTCY31_23 (325 aa), FASTA scores: opt: 1386, E(): 0,(69. 1% identity in 301 aa overlap). Contains possible N-terminal signal sequence.; Possible S-adenosylmethionine-dependent methyltransferase 341906 886618 Rv0281 Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_214795.1 340998 D 83332 CDS NP_214796.1 15607423 886613 342130..344025 1 NC_000962.3 Rv0282, (MTV035.10), len: 631 aa. eccA3, esx conserved component, ESX-3 type VII secretion system protein, similar to Y14967|MLCB628.18c hypothetical protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 916,E(): 0, (38.7% identity in 568 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Z94121|MTY15F10.26 (619 aa), FASTA scores: opt: 743, E(): 0, (29.9% identity in 612 aa overlap). Member of CFXQ, CBXP family - 9 members in Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; ESX conserved component EccA3 ESX-3 type VII secretion system protein 344025 eccA3 886613 eccA3 Mycobacterium tuberculosis H37Rv ESX conserved component EccA3 ESX-3 type VII secretion system protein NP_214796.1 342130 D 83332 CDS NP_214797.1 15607424 886645 344022..345638 1 NC_000962.3 Rv0283, (MTV035.11), len: 538 aa. eccB3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to several hypothetical mycobacterial proteins e.g. Z94121|MTY15F10_16|Rv3895c from Mycobacterium tuberculosis (495 aa), FASTA scores: opt: 698, E(): 0, (37.6% identity in 492 aa overlap); Rv1782; Rv3450c; Rv3869; and Y14967|MLCB628_16|MLCB628.17c from Mycobacterium leprae (481 aa), FASTA scores: opt: 672, E(): 1.5e-31, (37.2% identity in 506 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX conserved component EccB3 ESX-3 type VII secretion system protein Possible membrane protein 345638 eccB3 886645 eccB3 Mycobacterium tuberculosis H37Rv ESX conserved component EccB3 ESX-3 type VII secretion system protein Possible membrane protein NP_214797.1 344022 D 83332 CDS NP_214798.1 15607425 886611 345635..349627 1 NC_000962.3 Rv0284, (MTV035.12), len: 1330 aa. eccC3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to products of two adjacent Mycobacterium leprae genes, MLCB628.16c (744 aa) and MLCB628.15c (597 aa); and throughout its length to several large Mycobacterium tuberculosis proteins: Rv3447c,Rv3870, Rv1784, etc. Y14967|MLCB628_ 15 (744 aa), FASTA scores: opt: 942, E(): 0, (33.8% identity in 730 aa overlap); Y14967|MLCB628_14 (597 aa), FASTA scores: opt: 613, E(): 3.1e-30, (31.7% identity in 615 aa overlap); Z94121|MTY15F10_17 (1396 aa), FASTA scores: opt: 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap); Z95389|MTCY77_19 (1236 aa), FASTA scores: opt 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap). Contains three PS00017 ATP/GTP-binding site motif A (P-loop).; ESX conserved component EccC3 ESX-3 type VII secretion system protein Possible membrane protein 349627 eccC3 886611 eccC3 Mycobacterium tuberculosis H37Rv ESX conserved component EccC3 ESX-3 type VII secretion system protein Possible membrane protein NP_214798.1 345635 D 83332 CDS YP_177710.1 57116715 886608 349624..349932 1 NC_000962.3 Rv0285, (MTV035.13), len: 102 aa. PE5, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to others e.g. AL0212|MTV012_37 from Mycobacterium tuberculosis (105 aa), FASTA scores: opt: 497, E(): 2.6e-24, (80.4% identity in 102 aa overlap); Z80108|MTCY21B4.03 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 413, E(): 3.7e-19, (66.7% identity in 102 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; PE family protein PE5 349932 PE5 886608 PE5 Mycobacterium tuberculosis H37Rv PE family protein PE5 YP_177710.1 349624 D 83332 CDS YP_177711.1 57116716 886607 349935..351476 1 NC_000962.3 Rv0286, (MTV035.14), len: 513 aa. PPE4, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 958, E(): 0,(43.5% identity in 522 aa overlap).; PPE family protein PPE4 351476 PPE4 886607 PPE4 Mycobacterium tuberculosis H37Rv PPE family protein PPE4 YP_177711.1 349935 D 83332 CDS NP_214801.1 15607428 886604 351525..351818 1 NC_000962.3 Rv0287, (MTV035.15), len: 97 aa. EsxG, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to Rv3020c|AL0212|MTV012.34 (97 aa), FASTA scores: opt: 564,E(): 0, (91.8% identity in 97 aa overlap). Contains probable helix-turn-helix motif at aa 14-35 (Score 144,+4.11 SD). Seems to belong to the ESAT6 family (see Gey Van Pittius et al., 2001). Note that previously known as TB9.8.; ESAT-6 like protein EsxG (conserved protein TB9.8) 351818 esxG 886604 esxG Mycobacterium tuberculosis H37Rv ESAT-6 like protein EsxG (conserved protein TB9.8) NP_214801.1 351525 D 83332 CDS NP_214802.1 15607429 886603 351848..352138 1 NC_000962.3 Rv0288, (MT0301, MTV035.16), len: 96 aa. EsxH, low molecular weight protein antigen 7 (10 kDa antigen) (CFP-7) (Protein TB10.4) (see citations below), ala-rich protein; member of mycobacterial protein family containing ESAT-6,very similar to MTV012_33 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 566, E(): 0, (84.4% identity in 96 aa overlap). Alternative start codon possible position 351878 (see Rosenkrands et al., 2000). Belongs to the ESAT6 family (see Skjot et al., 2000; 2002; Gey Van Pittius et al., 2001). Note that previously known as cfp7 (alternate gene name: TB10.4). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al.,2008).; Low molecular weight protein antigen 7 EsxH (10 kDa antigen) (CFP-7) (protein TB10.4) 352138 esxH 886603 esxH Mycobacterium tuberculosis H37Rv Low molecular weight protein antigen 7 EsxH (10 kDa antigen) (CFP-7) (protein TB10.4) NP_214802.1 351848 D 83332 CDS NP_214803.1 15607430 886602 352149..353036 1 NC_000962.3 Rv0289, (MTV035.17), len: 295 aa. EspG3, ESX-3 secretion-associated protein, equivalent to CAC32061.1|AL583926 possible DNA-binding protein from Mycobacterium leprae (289 aa); and showing some similarity to Rv3866|G70656|CAB06238.1|Z94121|MTCY15F10.23 from Mycobacterium tuberculosis (276 aa), FASTA scores: opt: 149, E(): 0.0035, (27.7% identity in 289 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX-3 secretion-associated protein EspG3 353036 espG3 886602 espG3 Mycobacterium tuberculosis H37Rv ESX-3 secretion-associated protein EspG3 NP_214803.1 352149 D 83332 CDS NP_214804.1 15607431 886599 353083..354501 1 NC_000962.3 Rv0290, (MTV035.18), len: 472 aa. EccD3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein, similar to several others in mycobacteria e.g. Z95389|MTCY77_20|Rv3887c from Mycobacterium tuberculosis (467 aa), FASTA scores: opt: 429, E(): 5.1e-19, (28. 6% identity in 479 aa overlap); Rv3877; Rv1795; Rv3448; and Y14967|MLCB628_9|MLCB628.10c from Mycobacterium leprae (480 aa), FASTA scores: opt: 269,E(): 3.1e-09, (26.0% identity in 503 aa overlap).; ESX conserved component EccD3 ESX-3 type VII secretion system protein Probable transmembrane protein 354501 eccD3 886599 eccD3 Mycobacterium tuberculosis H37Rv ESX conserved component EccD3 ESX-3 type VII secretion system protein Probable transmembrane protein NP_214804.1 353083 D 83332 CDS NP_214805.1 15607432 886615 354498..355883 1 NC_000962.3 Rv0291, (MTV035.19), len: 461 aa. Probable mycP3,membrane-anchored serine protease (mycosin) (see Brown et al., 2000), similar to several others in mycobacteria e.g. Z94121|MTY15F10_28|Rv1796 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 1168, E(): 0, (44.6% identity in 453 aa overlap); Rv3886c; Rv3883c; Rv3449; and Y14967|MLCB628_4|MLCB628.04 from Mycobacterium leprae (446 aa), FASTA scores: opt: 1159, E(): 0, (43.5 identity in 446 aa overlap). Has signal sequence and hydrophobic stretch at C-terminus, followed by short positively charged segment,that seems to act as a membrane anchor. Contains PS00137 Serine proteases, subtilase family, histidine active site signature. Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable membrane-anchored mycosin MycP3 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-3) 355883 mycP3 886615 mycP3 Mycobacterium tuberculosis H37Rv Probable membrane-anchored mycosin MycP3 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-3) NP_214805.1 354498 D 83332 CDS NP_214806.1 15607433 886601 355880..356875 1 NC_000962.3 Rv0292, (MTV035.20), len: 331 aa. EccE3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein (has two hydrophobic segments at N-terminal end), equivalent to CAC32058.1|AL583926 conserved membrane protein from Mycobacterium leprae (339 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; ESX conserved component EccE3 ESX-3 type VII secretion system protein Probable transmembrane protein 356875 eccE3 886601 eccE3 Mycobacterium tuberculosis H37Rv ESX conserved component EccE3 ESX-3 type VII secretion system protein Probable transmembrane protein NP_214806.1 355880 D 83332 CDS NP_214807.1 15607434 886594 complement(356862..358064) 1 NC_000962.3 Rv0293c, (MTV035.21c), len: 400 aa. Conserved protein, similar in C-terminal part to Rv2627c|B70573|MTCY01A10.05|CAB08637.1|Z95387 conserved hypothetical protein from Mycobacterium tuberculosis (413 aa), FASTA scores: opt: 394, E(): 2.1e-17, (31.1% identity in 299 aa overlap).; hypothetical protein 358064 886594 Rv0293c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214807.1 356862 R 83332 CDS NP_214808.1 15607435 886593 358171..358956 1 NC_000962.3 Rv0294, (MTV035.22), len: 261 aa. Probable tam,trans-aconitate methyltransferase, similar to others e.g. P76145|TAM_ECOLI|7465793|B64906|B1519 trans-aconitate methyltransferase from Escherichia coli strain K12 (252 aa), FASTA scores: opt: 649, E(): 0, (39.3 identity in 252 aa overlap). Belongs to the methyltransferase superfamily.; Probable trans-aconitate methyltransferase Tam 358956 tam 886593 tam Mycobacterium tuberculosis H37Rv Probable trans-aconitate methyltransferase Tam NP_214808.1 358171 D 83332 CDS NP_214809.1 15607436 886596 complement(358945..359748) 1 NC_000962.3 Rv0295c, (MTV035.23c), len: 267 aa. Conserved protein, showing weak similarity with CAC46877.1|AL591790 conserved hypothetical protein from Sinorhizobium meliloti (213 aa); and NP_104818.1|14023999|BAB50604.1|AP00300 Protein with weak similarity to NodH from Mesorhizobium loti (257 aa). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 359748 886596 Rv0295c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214809.1 358945 R 83332 CDS YP_177712.1 57116717 886600 complement(359758..361155) 1 NC_000962.3 Rv0296c, (MTCY63.01c, MTV035.24c), len: 465 aa. Probable sulfatase, possibly an aryl-/steryl-sulfatase or a sulfamidase (sulfohydrolase) (sulphamidase). Similar to various hydrolases e.g. AAG41945.1|AF304053_1|AF304053 heparan N-sulfatase from Mus musculus (502 aa); NP_061292.1|6851181|AAF29460.1|AF153827_1|AF153827 N-sulfoglucosamine sulfohydrolase (sulfamidase) (sulphamidase) from Mus musculus (502 aa); AAG17206.1|AF217203_1|AF217203 heparan sulfate sulfamidase from Canis familiaris (507 aa); P08842|STS_HUMAN|1360652 steryl-sulfatase precursor (steroid sulfatase) (steryl-sulfate sulfohydrolase) (arylsulfatase C) (ASC) from Homo sapiens (583 aa); ARSB_FELCA|P33727 arylsulfatase B precursor (535 aa), FASTA scores: opt: 231, E(): 1.7e-08,(30.3% identity in 261 aa overlap). Also similarity with 4 others sulfatases in Mycobacterium tuberculosis. Contains sulfatases signature 1 (PS00523). Note that previously known as atsG.; Probable sulfatase 361155 886600 Rv0296c Mycobacterium tuberculosis H37Rv Probable sulfatase YP_177712.1 359758 R 83332 CDS YP_177713.1 57116718 885981 361334..363109 1 NC_000962.3 Rv0297, (MTCY63.02), len: 591 aa. PE_PGRS5, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Y03A_MYCTU|Q10637 from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 1884, E(): 0,(53.7% identity in 635 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE-PGRS family protein PE_PGRS5 363109 PE_PGRS5 885981 PE_PGRS5 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS5 YP_177713.1 361334 D 83332 CDS NP_214812.1 15607439 886590 363252..363479 1 NC_000962.3 Rv0298, (MTCY63.03), len: 75 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 363479 886590 Rv0298 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214812.1 363252 D 83332 CDS NP_214813.1 15607440 886598 363476..363778 1 NC_000962.3 Rv0299, (MTCY63.04), len: 100 aa. Hypothetical unknown protein. Equivalent to AAK44536.1 from Mycobacterium tuberculosis strain CDC1551 (49 aa) but longer 51 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 363778 886598 Rv0299 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214813.1 363476 D 83332 CDS NP_214814.1 15607441 886588 363826..364047 1 NC_000962.3 Rv0300, (MTCY63.05), len: 73 aa. Possible vapB2,antitoxin, part of toxin-antitoxin (TA) operon with Rv0301 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weak similarity with others e.g. Rv3697c from Mycobacterium tuberculosis (145 aa),Rv1721c|MTCY04C12.06c|Z81360|MTCY4C12_4 conserved hypothetical protein from Mycobacterium tuberculosis (75 aa), FASTA scores: opt: 84, E(): 8.3, (39.5% identity in 38 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible antitoxin VapB2 364047 vapB2 886588 vapB2 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB2 NP_214814.1 363826 D 83332 CDS NP_214815.1 15607442 886586 364044..364469 1 NC_000962.3 Rv0301, (MTCY63.06), len: 141 aa. Possible vapC2,toxin, part of toxin-antitoxin (TA) operon with Rv0300,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2757c, Rv0229c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC2 364469 vapC2 886586 vapC2 Mycobacterium tuberculosis H37Rv Possible toxin VapC2 NP_214815.1 364044 D 83332 CDS NP_214816.1 15607443 886584 364605..365237 1 NC_000962.3 Rv0302, (MTCY63.07), len: 210 aa. Probable transcription regulatory protein, TetR family (see citation below), with its N-terminus similar to N-terminus of several repressors and regulatory proteins of TetR/AcrR family e.g. ACRR_ECOLI|P34000 potential acraB operon repressor from Escherichia coli (215 aa), FASTA scores: opt: 172, E(): 3.1e-05, (22.7% identity in 194 aa overlap). Also similar in N-terminus to N-terminus of MTCY07A7.24 hypothetical regulator from Mycobacterium tuberculosis FASTA score: (38.7% identity in 62 aa overlap). Contains probable helix-turn helix motif from aa 35-56 (Score 1728,+5.07 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transcriptional regulatory protein (probably TetR/AcrR-family) 365237 886584 Rv0302 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR/AcrR-family) NP_214816.1 364605 D 83332 CDS NP_214817.1 15607444 886581 365234..366142 1 NC_000962.3 Rv0303, (MTCY63.08), len: 302 aa. Possible dehydrogenase/reductase, similar to various NADPH dehydrogenases and other NADPH oxidoreductases e.g. O48741|PORC_ARATH|7488284|T00897 protochlorophyllide reductase C chloroplast precursor (NADPH-protochlorophyllide oxidoreductase C) from Arabidopsis thaliana (401 aa); Q42850 NADPH dehydrogenase (395 aa), FASTA scores: opt: 347, E(): 3.8e-16, (35.4% identity in 319 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable dehydrogenase/reductase 366142 886581 Rv0303 Mycobacterium tuberculosis H37Rv Probable dehydrogenase/reductase NP_214817.1 365234 D 83332 CDS YP_177714.1 57116719 886592 complement(366150..372764) 1 NC_000962.3 Rv0304c, (MTCY63.9c), len: 2204 aa. PPE5, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR),similar to others e.g. Z95324|MTY13E10_16 from Mycobacterium tuberculosis (1443 aa), FASTA scores: E(): 0,(50.6% identity in 1403 aa overlap); Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 989, E(): 0, (42.3% identity in 522 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PPE family protein PPE5 372764 PPE5 886592 PPE5 Mycobacterium tuberculosis H37Rv PPE family protein PPE5 YP_177714.1 366150 R 83332 CDS YP_177715.1 57116720 885978 complement(372820..375711) 1 NC_000962.3 Rv0305c, (MTCY63.10c), len: 963 aa. PPE6, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR),similar to others e.g. Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 1340, E(): 0,(40.9% identity in 815 aa overlap).; PPE family protein PPE6 375711 PPE6 885978 PPE6 Mycobacterium tuberculosis H37Rv PPE family protein PPE6 YP_177715.1 372820 R 83332 CDS NP_214820.1 15607447 886577 375914..376585 1 NC_000962.3 Rv0306, (MTCY63.11), len: 223 aa. Putative oxidoreductase, highly similar to H83485|9947208|AAG04663.1|AE004557_4|AE004557 conserved hypothetical protein from Pseudomonas aeruginosa strain PAO1 (218 aa); and to other putative oxidoreductases e.g. middle part of CAB76073.1|AL157953 putative nitroreductase from Streptomyces coelicolor (1212 aa); Q52685|BLUB protein involved in cobalamin (vitamin B12) synthesis from Rhodobacter capsulatus (206 aa), FASTA scores: opt: 318,E(): 2e-15, (35.6% identity in 191 aa overlap).; Putative oxidoreductase 376585 886577 Rv0306 Mycobacterium tuberculosis H37Rv Putative oxidoreductase NP_214820.1 375914 D 83332 CDS NP_214821.1 15607448 886580 complement(376573..377055) 1 NC_000962.3 Rv0307c, (MTCY63.12c), len: 160 aa. Unknown protein.; hypothetical protein 377055 886580 Rv0307c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214821.1 376573 R 83332 CDS NP_214822.1 15607449 886583 377113..377829 1 NC_000962.3 Rv0308, (MTCY63.13), len: 238 aa. Probable conserved integral membrane protein, with C-terminus highly similar to C-terminus of other integral membrane proteins or phosphatases e.g. AAK25788.1|AF336822_1|13430250|AAK25789.1|AF336823_1 putative phosphatase from Streptococcus pyogenes (201 aa); Q06074 hypothetical 24.9 kDa protein (216 aa), FASTA scores: opt: 209, E(): 2e-07, (27.9% identity in 140 aa overlap). Could be a phosphatase.; Probable conserved integral membrane protein 377829 886583 Rv0308 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_214822.1 377113 D 83332 CDS NP_214823.1 15607450 886574 377931..378587 1 NC_000962.3 Rv0309, (MTCY63.14), len: 218 aa. Possible conserved exported protein (has putative N-terminal signal sequence),equivalent to AC32053.1|AL583926 putative secreted protein from Mycobacterium leprae (218 aa). Also similar to others e.g. AB76092.1|AL157956 putative secreted protein from Streptomyces coelicolor (238 aa). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved exported protein 378587 886574 Rv0309 Mycobacterium tuberculosis H37Rv Possible conserved exported protein NP_214823.1 377931 D 83332 CDS NP_214824.1 15607451 886570 complement(378657..379148) 1 NC_000962.3 Rv0310c, (MTCY63.15c), len: 163 aa. Conserved protein, similar to some bile acid dehydratases e.g. P19412|BAIE_EUBSP|98749|D37844|1381566|AAC45413.1|U57489 bile acid-inducible operon protein E from Eubacterium sp (166 aa), FASTA scores: opt: 302, E(): 1e-11, (38.8% identity in 134 aa overlap); AAF22847.1|AF210152_4 bile acid 7a-dehydratase from Clostridium sp. (168 aa).; hypothetical protein 379148 886570 Rv0310c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214824.1 378657 R 83332 CDS NP_214825.1 15607452 886579 379172..380401 1 NC_000962.3 Rv0311, (MTCY63.16), len: 409 aa. Unknown protein. Contains PS00881 Protein splicing signature.; hypothetical protein 380401 886579 Rv0311 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214825.1 379172 D 83332 CDS NP_214826.1 15607453 886566 380556..382418 1 NC_000962.3 Rv0312, (MTCY63.17), len: 620 aa. Conserved hypothetical protein with highly Pro-, Thr-rich C-terminus. Similar to Pro-,Thr-rich region in Rv2264c|AL021925|MTV022_14 from Mycobacterium tuberculosis (592 aa), FASTA scores: opt: 1075, E(): 0, (38.9% identity in 627 aa overlap). Also some similarity with Rv0350|dnaK from Mycobacterium tuberculosis. Possibly membrane protein; has hydrophobic stetch in its middle part.; Conserved hypothetical proline and threonine rich protein 382418 886566 Rv0312 Mycobacterium tuberculosis H37Rv Conserved hypothetical proline and threonine rich protein NP_214826.1 380556 D 83332 CDS NP_214827.1 15607454 886572 382490..382876 1 NC_000962.3 Rv0313, (MTCY63.18), len: 128 aa. Conserved protein,equivalent only to CAC32049.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (130 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 382876 886572 Rv0313 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214827.1 382490 D 83332 CDS NP_214828.1 15607455 886564 complement(382879..383541) 1 NC_000962.3 Rv0314c, (MTCY63.19c), len: 220 aa. Possible conserved membrane protein, with hydrophobic stretch from residues ~75-100. Similar in C-terminal part to Mycobacterium tuberculosis proteins Rv0679c and Rv0680c.; Possible conserved membrane protein 383541 886564 Rv0314c Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_214828.1 382879 R 83332 CDS NP_214829.1 15607456 886563 383602..384486 1 NC_000962.3 Rv0315, (MTCY63.20), len: 294 aa. Possible beta-1,3-glucanase precursor (has hydrophobic stretch in its N-terminal part), similar to others e.g. Q51333|AAC44371.1 beta-1,3-glucanase II a from Oerskovia xanthineolytica (306 aa), FASTA scores: opt: 76, E(): 3e-14, (34.1% identity in 302 aa overlap); and AAC38290.1|AF052745 beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa). Contains glycosyl hydrolases family 16 active site signature (PS01034).; Possible beta-1,3-glucanase precursor 384486 886563 Rv0315 Mycobacterium tuberculosis H37Rv Possible beta-1,3-glucanase precursor NP_214829.1 383602 D 83332 CDS NP_214830.1 15607457 886560 384535..385149 1 NC_000962.3 Rv0316, (MTCY63.21), len: 204 aa. Possible muconolactone isomerase, showing weak similarity with some muconolactone isomerases e.g. O33947|CTC1_ACILW muconolactone delta-isomerase 1 (MIASE 1)(96 aa), FASTA scores: opt: 179, E(): 3.9e-05, (32.6% identity in 92 aa overlap).; Possible muconolactone isomerase 385149 886560 Rv0316 Mycobacterium tuberculosis H37Rv Possible muconolactone isomerase NP_214830.1 384535 D 83332 CDS NP_214831.1 15607458 886559 complement(385173..385943) 1 NC_000962.3 Rv0317c, (MTCY63.22c), len: 256 aa (start uncertain,chosen by homology). Possible glpQ2, glycerophosphoryl diester phosphodiesterase, similar to others e.g. E75317|6459876|AAF11631.1|AE002044_4 glycerophosphoryl diester phosphodiesterase from Deinococcus radiodurans (285 aa); P10908|UGPQ_ECOLI from Escherichia coli (247 aa),FASTA scores: opt: 220, E(): 5.2e-07, (28.0% identity in 250 aa overlap). Also similar to MTCY01A6.27 from Mycobacterium tuberculosis FASTA score: (27.5% identity in 247 aa overlap).; Possible glycerophosphoryl diester phosphodiesterase GlpQ2 (glycerophosphodiester phosphodiesterase) 385943 glpQ2 886559 glpQ2 Mycobacterium tuberculosis H37Rv Possible glycerophosphoryl diester phosphodiesterase GlpQ2 (glycerophosphodiester phosphodiesterase) NP_214831.1 385173 R 83332 CDS YP_177716.1 57116721 886576 complement(386305..387099) 1 NC_000962.3 Rv0318c, (MTCY63.23c), len: 264 aa. Probable conserved integral membrane protein, with some similarity to C-terminus of GUFA_MYXXA|Q06916 (254 aa), FASTA scores: opt: 157, E (): 0.0032, (28.3% identity in 198 aa overlap). Also similar to O26573 conserved protein from Methanobacterium thermoauto (259 aa), FASTA scores: opt: 173, E(): 5.2e-05, (32.7% identity in 214 aa overlap).; Probable conserved integral membrane protein 387099 886576 Rv0318c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein YP_177716.1 386305 R 83332 CDS NP_214833.1 15607460 886555 387148..387816 1 NC_000962.3 Rv0319, (MTCY63.24), len: 222 aa. Probable pcp,pyrrolidone-carboxylate peptidase, highly similar to others e.g. PCP_PSEFL|P42673 pyrrolidone-carboxylate peptidase from Pseudomonas fluorescens (213 aa), FASTA scores: opt: 478, E(): 7.5e-25, (40.2% identity in 219 aa overlap). Belongs to peptidase family C15 (thiol protease).; Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase) 387816 pcp 886555 pcp Mycobacterium tuberculosis H37Rv Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase) NP_214833.1 387148 D 83332 CDS NP_214834.1 15607461 886553 387888..388550 1 NC_000962.3 Rv0320, (MTCY63.25), len: 220 aa. Possible conserved exported protein, similar to some hypothetical proteins and to the middle part of a peptidase: NP_066789.1|10657900|AAG21739.1|AF116907 putative peptidase from Rhodococcus equi (546 aa). Also similar to Rv1728c|MTCY04C12.13c from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 497, E(): 1.2e-26, (41.8% identity in 225 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved exported protein 388550 886553 Rv0320 Mycobacterium tuberculosis H37Rv Possible conserved exported protein NP_214834.1 387888 D 83332 CDS NP_214835.1 15607462 886552 388582..389154 1 NC_000962.3 Rv0321, (MTCY63.26), len: 190 aa. Probable dcd (alternate gene names: dus or paxA), deoxycytidine triphosphate deaminase, equivalent to CAC32024.1|AL583925 probable deoxycytidine triphosphate deaminase from Mycobacterium leprae (190 aa). Also highly similar to others e.g. Q9X8W0|DCD_STRCO|7480599|T36613|SCH35.46 deoxycytidine triphosphate deaminase from Streptomyces coelicolor (191 aa); DCD_ECOLI|P28248|DUS|PAXA|B2065 deoxycytidine triphosphate deaminase from Escherichia coli strain K12 (193 aa), FASTA scores: opt: 408, E(): 2.7e-21,(43.1% identity in 188 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the dCTP deaminase family. The transcription of this CDS seems to be activated specifically in host granulomas (see citation below).; Probable deoxycytidine triphosphate deaminase Dcd (dCTP deaminase) 389154 dcd 886552 dcd Mycobacterium tuberculosis H37Rv Probable deoxycytidine triphosphate deaminase Dcd (dCTP deaminase) NP_214835.1 388582 D 83332 CDS NP_214836.1 15607463 886550 389260..390591 1 NC_000962.3 Rv0322, (MTCY63.27), len: 443 aa. Probable udg (alternate gene name: rkpK), UDP-glucose 6-dehydrogenase ,highly similar to others e.g. CAC44517.1|AL596138 putative UDP-glucose 6-dehydrogenase from Streptomyces coelicolor (447 aa); Q56812 UDP-glucose dehydrogenase from Xanthomonas campestris (445 aa), FASTA scores: opt: 713, E(): 0, (41.9% identity in 351 aa overlap); etc. Also similar to several GDP-mannose 6-dehydrogenase. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UDP-glucose/GDP-mannose dehydrogenases family.; Probable UDP-glucose 6-dehydrogenase UdgA (UDP-GLC dehydrogenase) (UDP-GLCDH) (UDPGDH) 390591 udgA 886550 udgA Mycobacterium tuberculosis H37Rv Probable UDP-glucose 6-dehydrogenase UdgA (UDP-GLC dehydrogenase) (UDP-GLCDH) (UDPGDH) NP_214836.1 389260 D 83332 CDS NP_214837.1 15607464 886557 complement(390580..391251) 1 NC_000962.3 Rv0323c, (MTCY63.28c), len: 223 aa. Conserved hypothetical protein, similar to others e.g. YPJG_BACSU|P42981 hypothetical 24.8 kDa protein from Bacillus subtilis (224 aa), FASTA scores: opt: 182, E(): 1.3e-05, (27.5% identity in 211 aa overlap). Also some similarity to MLU15183_8 from Mycobacterium tuberculosis FASTA score: (32.0% identity in 147 aa overlap). Alternative nucleotide at position 390828 (T->C; S142G) has been observed. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 391251 886557 Rv0323c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214837.1 390580 R 83332 CDS NP_214838.1 15607465 886548 391352..392032 1 NC_000962.3 Rv0324, (MTCY63.29), len: 226 aa. Possible transcriptional regulator, arsR family, with its N-terminus similar to the N-terminus of other DNA-binding proteins e.g. P30346|MERR_STRLI probable mercury resistance operon from Streptomyces lividans (125 aa), FASTA scores: opt: 154, E(): 0.002, (32.2% identity in 90 aa overlap), and its C-terminal part similar to hypothetical bacterial proteins e.g. P54510|YQHL_BACSU hypothetical 14.6 kDa protein from Bacillus subtilis (126 aa), FASTA scores: opt: 159, E(): 0.00097, (35.5% identity in 76 aa overlap). Most similar to AJ005575|SPE005575_2 ORF1 required for antibiotic production from Streptomyces peucetius (226 aa), FASTA scores: opt: 816, E(): 0, (60.7% identity in 211 aa overlap). Also similar in C-terminus to MTCY164.26 molybdopterin biosynthesis moeb protein from Mycobacterium tuberculosis FASTA score: (36.8% identity in 114 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible transcriptional regulatory protein (possibly ArsR-family) 392032 886548 Rv0324 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (possibly ArsR-family) NP_214838.1 391352 D 83332 CDS NP_214839.1 15607466 886546 392039..392263 1 NC_000962.3 Rv0325, (MTCY63.30), len: 74 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 392263 886546 Rv0325 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214839.1 392039 D 83332 CDS NP_214840.1 15607467 886544 392273..392728 1 NC_000962.3 Rv0326, (MTCY63.31), len: 151 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 392728 886544 Rv0326 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214840.1 392273 D 83332 CDS NP_214841.1 15607468 886538 complement(392696..394045) 1 NC_000962.3 Rv0327c, (MT0342, MTCY63.32c), len: 449 aa. Possible cyp135A1, cytochrome P450, similar to cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. FQ12609 putative P450 monooxygenase (506 aa), FASTA scores: opt: 276, E() : 1.7e-11, (27.9% identity in 433 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTV039.06|Rv0568 putative cytochrome P450 (472 aa); MTCI5.10 cytochrome p450 FASTA score: (30.4% identity in 434 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. Alternative start possible at 33706 but no RBS. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible cytochrome P450 135A1 Cyp135A1 394045 cyp135A1 886538 cyp135A1 Mycobacterium tuberculosis H37Rv Possible cytochrome P450 135A1 Cyp135A1 NP_214841.1 392696 R 83332 CDS NP_214842.1 15607469 886542 394111..394713 1 NC_000962.3 Rv0328, (MTCY63.33), len: 200 aa. Possible transcription regulator, TetR/acrR family, similar in part to various hypothetical transcriptional regulators e.g. T36696|4726006|CAB41735.1|AL049731 probable regulatory protein from Streptomyces coelicolor (197 aa). Also some similarity with YX44_MYCTU|Q10829 hypothetical transcriptional regulator from Mycobacterium tuberculosis (195 aa), FASTA scores: opt: 154, E(): 0.00061, (26.7% identity in 202 aa overlap). Contains probable helix-turn helix motif from aa 27-48 (Score 1408, +3.98 SD). Seems to belong to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible transcriptional regulatory protein (possibly TetR/AcrR-family) 394713 886542 Rv0328 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (possibly TetR/AcrR-family) NP_214842.1 394111 D 83332 CDS NP_214843.1 15607470 886536 complement(394694..395320) 1 NC_000962.3 Rv0329c, (MTCY63.34c), len: 208 aa. Conserved hypothetical protein, showing some similarity with others hypothetical proteins and methyltransferases e.g. MitM|AF127374_14 methyltransferase from Streptomyces lavendulae (283 aa), FASTA scores: opt: 242, E(): 1.8e-08,(37.2% identity in 145 aa overlap); Q48938 from Methanosarcina barkeri (262 aa), FASTA scores: opt: 194,E(): 3.6e-06, (31.1% identity in 119 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 395320 886536 Rv0329c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214843.1 394694 R 83332 CDS NP_214844.1 15607471 886540 complement(395347..396087) 1 NC_000962.3 Rv0330c, (MTCY63.35c), len: 246 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 396087 886540 Rv0330c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214844.1 395347 R 83332 CDS NP_214845.1 15607472 886534 396201..397367 1 NC_000962.3 Rv0331, (MTCY63.36), len: 388 aa. Possible dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_103779.1|14022957|BAB49565.1|AP002999 flavoprotein reductase from Mesorhizobium loti (377 aa); NP_147681.1 predicted NAD(FAD)-dependent dehydrogenase from Aeropyrum pernix (381 aa); DHSU_CHRVI|Q06530 sulfide dehydrogenase (431 aa), FASTA scores: opt: 347, E(): 6.8e-15, (25.6% identity in 348 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible dehydrogenase/reductase 397367 886534 Rv0331 Mycobacterium tuberculosis H37Rv Possible dehydrogenase/reductase NP_214845.1 396201 D 83332 CDS NP_214846.1 15607473 886532 397442..398227 1 NC_000962.3 Rv0332, (MTCY63.37), len: 261 aa. Conserved protein,similar to several conserved hypothetical proteins from Streptomyces coelicolor e.g. SC6A9.18c|AL031035|SC6A9_18|T35449 hypothetical protein (266 aa), FASTA scores: opt: 508, E(): 5.7e-27, (36.7% identity in 251 aa overlap).; hypothetical protein 398227 886532 Rv0332 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214846.1 397442 D 83332 CDS NP_214847.1 15607474 886528 398254..398628 1 NC_000962.3 Rv0333, (MTCY63.38), len: 124 aa. Unknown protein.; hypothetical protein 398628 886528 Rv0333 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214847.1 398254 D 83332 CDS NP_214848.1 15607475 886568 398658..399524 1 NC_000962.3 Rv0334, (MTCY279.01), len: 288 aa. RmlA (alternate gene name: rfbA), alpha-D-glucose-1-phosphate thymidylyl-transferase (see citations below), equivalent to CAC32020.1|AL583925 glucose-1-phosphate thymidyltransferase from Mycobacterium leprae (288 aa). Also highly similar to others e.g. AAG29804.1|AF235050 glucose-1-phosphate thymidylyltransferase from Streptomyces rishiriensis (296 aa); RBA1_ECOLI|P37744 glucose-1-phosphate thymidylyltransferase from Escherichia coli strain K12 (293 aa), FASTA scores: opt: 1199, E(): 0, (62.0% identity in 284 aa overlap). Belongs to the glucose-1-phosphate thymidylyltransferase family.; Alpha-D-glucose-1-phosphate thymidylyltransferase RmlA (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase) 399524 rmlA 886568 rmlA Mycobacterium tuberculosis H37Rv Alpha-D-glucose-1-phosphate thymidylyltransferase RmlA (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase) NP_214848.1 398658 D 83332 CDS YP_177717.1 57116722 886527 complement(399535..400050) 1 NC_000962.3 Rv0335c, (MTCY279.02c), len: 171 aa. PE6, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002); contains short region of similarity to part of the unique N-terminus of the Mycobacterium tuberculosis PGRS family of Glycine-rich proteins e.g. Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kd protein (603 aa), FASTA scores: opt: 219, E(): 1.1e-08, (51.5% identity in 66 aa overlap).; PE family protein PE6 400050 PE6 886527 PE6 Mycobacterium tuberculosis H37Rv PE family protein PE6 YP_177717.1 399535 R 83332 CDS NP_214850.1 15607477 886524 400192..401703 1 NC_000962.3 Rv0336, (MTCY279.03), len: 503 aa. Part of Mycobacterium tuberculosis 13E12 repeat family; almost identical to Rv0515|MTCY20G10.05 hypothetical protein from Mycobacterium tuberculosis FASTA scores: (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1148c, Rv1945, etc.; Conserved 13E12 repeat family protein 401703 886524 Rv0336 Mycobacterium tuberculosis H37Rv Conserved 13E12 repeat family protein NP_214850.1 400192 D 83332 CDS NP_214851.1 15607478 886522 complement(401873..403162) 1 NC_000962.3 Rv0337c, (MTCY279.04c), len: 429 aa. Probable aspC,aspartate aminotransferase (transaminase A), equivalent to CAC32019.1|AL583925 probable aspartate aminotransferase from Mycobacterium leprae (437 aa). Also highly similar to many e.g. Q48143|U32823 aspartate aminotransferase (404 aa), FASTA scores: opt: 1646, E(): 0, (57.2% identity in 404 aa overlap). Also some similarity to Rv3565|MTCY06G11.12 from Mycobacterium tuberculosis FASTA score: (27.2% identity in 383 aa overlap). Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate.; Probable aspartate aminotransferase AspC (transaminase A) (ASPAT) 403162 aspC 886522 aspC Mycobacterium tuberculosis H37Rv Probable aspartate aminotransferase AspC (transaminase A) (ASPAT) NP_214851.1 401873 R 83332 CDS NP_214852.1 15607479 886520 complement(403193..405841) 1 NC_000962.3 Rv0338c, (MTCY279.05c), len: 882 aa. Probable iron-sulphur-binding reductase, possibly membrane-bound,equivalent to CAC32018.1|AL583925 probable iron-sulphur-binding reductase from Mycobacterium leprae (880 aa). Also highly similar to others e.g. T36608|5019323|CAB44376.1|AL078610 probable iron-sulfur-binding reductase from Streptomyces coelicolor (760 aa), FASTA scores: opt: 1658, E(): 0, (49.9% identity in 772 aa overlap); BAB07521.1|AP001520 iron-sulphur-binding reductase from Bacillus halodurans (700 aa). Contains PS00070 Aldehyde dehydrogenases cysteine active site and two of PS00198 4Fe-4S ferredoxins,iron-sulfur binding region signature. First of several possible start sites chosen.; Probable iron-sulfur-binding reductase 405841 886520 Rv0338c Mycobacterium tuberculosis H37Rv Probable iron-sulfur-binding reductase NP_214852.1 403193 R 83332 CDS NP_214853.1 15607480 886516 complement(405950..408448) 1 NC_000962.3 Rv0339c, (MTCY279.06c), len: 832 aa. Possible transcriptional regulator, showing very weak similarity with parts of others. Contains PS00017 ATP/GTP-binding site motif A (P-loop); and probable helix-turn helix motif from aa 778-799 (Score 1041, +2.73 SD).; Possible transcriptional regulatory protein 408448 886516 Rv0339c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214853.1 405950 R 83332 CDS NP_214854.1 15607481 886514 408634..409173 1 NC_000962.3 Rv0340, (MTCY279.07), len: 179 aa. Conserved protein; MEME-mast analysis shows similarity to product of downstream gene, Rv0341|iniB.; hypothetical protein 409173 886514 Rv0340 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214854.1 408634 D 83332 CDS NP_214855.1 15607482 886518 409362..410801 1 NC_000962.3 Rv0341, (MTCY13E10.01), len: 479 aa. IniB,isoniazid-inducible gene, (see citations below). Protein very Gly-, Ala-rich, similar to cell wall proteins e.g. P27483|GRP_ARATH glycine-rich cell wall structural protein from A.thaliana (338 aa), FASTA scores: opt: 532, E(): 5.2e-13, (39.3% identity in 321 aa overlap). MEME-mast analysis shows similarity to product of upstream gene,Rv0340.; Isoniazid inductible gene protein IniB 410801 iniB 886518 iniB Mycobacterium tuberculosis H37Rv Isoniazid inductible gene protein IniB NP_214855.1 409362 D 83332 CDS NP_214856.1 15607483 886510 410838..412760 1 NC_000962.3 Rv0342, iniA, (MTCY13E10.02), len: 640 aa. IniA,isoniazid-inducible gene, (see citations below). Shows slight similarity to some hypothetical bacterial proteins e.g. P40983|YOR6_THER hypothetical protein (402 aa), FASTA scores: opt: 242, E(): 1.4e-07, (22.3% identity in 349 aa overlap). Also some similarity to downstream ORF Rv0343|iniC. Possible transmembrane stretch around residue 490. Alternative start site exists at 410824. Contains a phosphopantetheine attachment site motif suggestive of an acyl carrier protein. Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid.; Isoniazid inductible gene protein IniA 412760 iniA 886510 iniA Mycobacterium tuberculosis H37Rv Isoniazid inductible gene protein IniA NP_214856.1 410838 D 83332 CDS NP_214857.1 15607484 886508 412757..414238 1 NC_000962.3 Rv0343, (MTCY13E10.03), len: 493 aa. IniC,isoniazid-inducible gene, (see citations below). Shows slight similarity to P40983|YOR6_THER8 hypothetical protein (402 aa), FASTA scores: opt: 196, E(): 2.6e-05, (25.9% identity in 228 aa overlap). Also some similarity to upstream ORF Rv0342|iniA. Contains (PS00017) ATP/GTP-binding site motif A (P-loop). Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid.; Isoniazid inductible gene protein IniC 414238 iniC 886508 iniC Mycobacterium tuberculosis H37Rv Isoniazid inductible gene protein IniC NP_214857.1 412757 D 83332 CDS NP_214858.1 15607485 886512 complement(414381..414941) 1 NC_000962.3 Rv0344c, (MTCY13E10.04c), len: 186 aa. Probable lipoprotein, without homology. Has an appropriately positioned prokaryotic lipoprotein signature (PS00013).; Probable lipoprotein LpqJ 414941 lpqJ 886512 lpqJ Mycobacterium tuberculosis H37Rv Probable lipoprotein LpqJ NP_214858.1 414381 R 83332 CDS NP_214859.1 15607486 886505 415050..415460 1 NC_000962.3 Rv0345, (MTCY13E10.05), len: 136 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL13282 4|SCAH10_9 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 188,E(): 1.5e-05, (41.0% identity in 117 aa overlap).; hypothetical protein 415460 886505 Rv0345 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214859.1 415050 D 83332 CDS YP_177718.1 57116723 886530 complement(415502..416965) 1 NC_000962.3 Rv0346c, (MTCY13E10.06c), len: 487 aa. Possible ansP2, L-asparagine permease, integral membrane protein belonging to family containing many amino acid permeases,highly similar to G467030|B2126_F2_85|NP_301937.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (498 aa); and NP_301938.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (505 aa). Also highly similar to others e.g. P77610|ANSP_ECOLI L-asparagine permease from Escherichia coli strain K-12 (499 aa). Also highly similar to ANSP1|Rv2127|MT2186|MTCY261_22|O33261 probable L-asparagine permease from Mycobacterium tuberculosis (489 aa), FASTA score: (72.1% identity in 473 aa overlap). And shows some similarity to MTCY3G12.14 from Mycobacterium tuberculosis. Belongs to the amino acid permease family (APC family). Note that previously known as aroP2.; Possible L-asparagine permease AnsP2 (L-asparagine transport protein) 416965 ansP2 886530 ansP2 Mycobacterium tuberculosis H37Rv Possible L-asparagine permease AnsP2 (L-asparagine transport protein) YP_177718.1 415502 R 83332 CDS NP_214861.1 15607488 886501 417304..418290 1 NC_000962.3 Rv0347, (MTCY13E10.07), len: 328 aa (alternative start possible). Probable conserved membrane protein,similar to Rv0831c|AL022004|MTV043_23 from Mycobacterium tuberculosis (271 aa), FASTA scores: E(): 9.6e-21, (33.1% identity in 266 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable conserved membrane protein 418290 886501 Rv0347 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214861.1 417304 D 83332 CDS NP_214862.1 15607489 886500 418293..418946 1 NC_000962.3 Rv0348, (MTCY13E10.08), len: 217 aa. Possible transcriptional regulator, showing some similarity to O53334|RV3188|MTV014.32 conserved hypothetical protein from Mycobacterium tuberculosis (115 aa), FASTA score: (30.0% identity in 100 aa overlap). Contains probable helix-turn helix motif from aa 89-110 (Score 1407, +3.98 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible transcriptional regulatory protein 418946 886500 Rv0348 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214862.1 418293 D 83332 CDS NP_214863.1 15607490 886506 418949..419608 1 NC_000962.3 Rv0349, (MTCY13E10.09), len: 219 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 419608 886506 Rv0349 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214863.1 418949 D 83332 CDS NP_214864.1 15607491 885946 419835..421712 1 NC_000962.3 Rv0350, (MTCY13E10.10), len: 625 aa. Probable dnaK (alternate gene name: hsp70), 70 kDa heat shock protein (see citations below), equivalent to AAA25362.1|M95576|1924344A|738248 heat shock protein 70 from Mycobacterium leprae (621 aa); and DNAK_MYCPA|Q00488 (623 aa), FASTA scores: opt: 3678, E(): 0, (92.3% identity in 625 aa overlap). Also highly similar to others e.g. Q05558|DNAK_STRCO|453231|CAA54606.1|X77458 chaperone protein DNAK from Streptomyces coelicolor (618 aa). Has probably an ATPase activity. Note that this sequence differs from DNAK_MYCTU|P32723 (609 aa), due to a frameshift near the N-terminus. Belongs to the heat shock protein 70 family.; Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70) 421712 dnaK 885946 dnaK Mycobacterium tuberculosis H37Rv Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70) NP_214864.1 419835 D 83332 CDS NP_214865.1 15607492 886497 421709..422416 1 NC_000962.3 Rv0351, (MTCY13E10.11), len: 235 aa. Probable grpE protein (HSP-70 cofactor), equivalent to CAC32012.1|AL583925 Hsp70 cofactor from Mycobacterium leprae (229 aa). Also highly similar to others eg Q05562|GRPE_STRCO|2127521|PN0643 GRPE protein from Streptomyces coelicolor (225 aa). Contains grpE protein signature (PS01071). Belongs to the GrpE family.; Probable GrpE protein (HSP-70 cofactor) 422416 grpE 886497 grpE Mycobacterium tuberculosis H37Rv Probable GrpE protein (HSP-70 cofactor) NP_214865.1 421709 D 83332 CDS YP_177719.1 57116724 886495 422452..423639 1 NC_000962.3 Rv0352, (MTCY13E10.12), len: 395 aa. Probable dnaJ1,chaperone protein, equivalent to AAA25363.1|M95576 DNA J heatshock protein from Mycobacterium leprae (389 aa). Also highly similar to others. Contains both DnaJ signatures (PS00636, and PS00637). Belongs to the DNAJ family. Cofactor: binds two zinc ions per monomer. Note that sequence differs from DNAJ_MYCTU|P07881 due to a frameshift at the N-terminus. Note that previously known as dnaJ.; Probable chaperone protein DnaJ1 423639 dnaJ1 886495 dnaJ1 Mycobacterium tuberculosis H37Rv Probable chaperone protein DnaJ1 YP_177719.1 422452 D 83332 CDS NP_214867.1 15607494 885929 423639..424019 1 NC_000962.3 Rv0353, (MTCY13E10.13), len: 126 aa. Probable hspR,heat shock regulatory protein (see Stewart et al., 2001),merR family, highly similar to others e.g. HspR|P40183 heat shock regulatory protein from Streptomyces coelicolor (151 aa), FASTA scores: E(): 4.9e-22, (55.7% identity in 140 aa overlap), that binds to three inverted repeats (IR1-IR3) in the promoter region of the dnaK operon. Has possible coiled coil region in C-terminal half. Belongs to the MerR family of transcriptional regulators.; Probable heat shock protein transcriptional repressor HspR (MerR family) 424019 hspR 885929 hspR Mycobacterium tuberculosis H37Rv Probable heat shock protein transcriptional repressor HspR (MerR family) NP_214867.1 423639 D 83332 CDS YP_177720.1 57116725 886498 complement(424269..424694) 1 NC_000962.3 Rv0354c, (MTCY13E10.14c), len: 141 aa. PPE7, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. MTCY63_9 from Mycobacterium tuberculosis (2411 aa), FASTA scores: E(): 3.6e-11, (47.6% identity in 103 aa overlap). Possible continuation of ORF upstream, but no sequence error apparent.; PPE family protein PPE7 424694 PPE7 886498 PPE7 Mycobacterium tuberculosis H37Rv PPE family protein PPE7 YP_177720.1 424269 R 83332 CDS YP_177721.2 448824742 886491 complement(424777..434679) 1 NC_000962.3 Rv0355c, (MTCY13E10.15c, MTCY13E10.16c,MTCY13E10.17c), len: 3300 aa. PPE8, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL009198|MTV004_5 from Mycobacterium tuberculosis (3716 aa), FASTA scores: opt: 2906, E(): 0, (40.9% identity in 3833 aa overlap); MTV004_3 FASTA scores: (39.0% identity in 3531 aa overlap); etc. Gene contains large number of clustered Major Polymorphic Tandem Repeats (MPTR). Related to MTCY13E10.16c, E(): 0; MTCY13E10.17c, E(): 0; MTCY48.17,E(): 0; MTCY98.0034c, E(): 0; MTCY03C7.23 E(): 0; MTCY98.0031c, E(): 0; MTCY31.06c, E(): 5.6e-17; MTCY359.33,E(): 2.3e-16. Nucleotide position 426909 in the genome sequence has been corrected, A:C resulting in W2591G.; PPE family protein PPE8 434679 PPE8 886491 PPE8 Mycobacterium tuberculosis H37Rv PPE family protein PPE8 YP_177721.2 424777 R 83332 CDS NP_214870.1 15607497 886490 complement(434830..435474) 1 NC_000962.3 Rv0356c, (MTCY13E10.18c), len: 214 aa. Conserved protein, equivalent to AL023514|MLCB4_12 conserved hypothetical protein from Mycobacterium leprae (218 aa),FASTA scores: opt: 1067, E(): 0, (73.4% identity in 214 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 435474 886490 Rv0356c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214870.1 434830 R 83332 CDS NP_214871.1 15607498 886484 complement(435471..436769) 1 NC_000962.3 Rv0357c, (MTCY13E10.19c), len: 432 aa. Probable purA, adenylosuccinate synthase, equivalent to AL023514|MLCB4_13 from adenylosuccinate synthetase Mycobacterium leprae (432 aa), FASTA scores: opt: 2555,E(): 0, (87.9% identity in 431 aa overlap). Also highly similar to many bacterial adenylosuccinates synthetases e.g. P12283|PURA_ECOLI adenylosuccinates synthetase from Escherichia coli (431 aa), FASTA scores: E(): 0, (51.1% identity in 425 aa overlap); etc. Belongs to the adenylosuccinate synthetase family.; Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase) 436769 purA 886484 purA Mycobacterium tuberculosis H37Rv Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase) NP_214871.1 435471 R 83332 CDS NP_214872.1 15607499 886493 436860..437507 1 NC_000962.3 Rv0358, (MTCY13E10.20), len: 215 aa. Conserved protein, highly similar to ML0281|AL023514|MLCB4_14 conserved hypothetical protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 852, E(): 0, (62.9% identity in 229 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 437507 886493 Rv0358 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214872.1 436860 D 83332 CDS NP_214873.1 15607500 886482 437518..438297 1 NC_000962.3 Rv0359, (MTCY13E10.21), len: 259 aa. Probable conserved integral membrane protein, highly similar to hypothetical or other membrane proteins e.g. AL133220|SCC75A_6|T50569 probable membrane protein from Streptomyces coelicolor (265 aa), FASTA scores: opt: 642,E(): 0, (43.1% identity in 248 aa overlap); P70995 hypothetical 24.7 kDa protein from Bacillus subtilis (219 aa), FASTA scores: E(): 1.5e-12, (31.3% identity in 192 aa overlap). Contains neutral zinc metallopeptidases,zinc-binding region signature (PS00142).; Probable conserved integral membrane protein 438297 886482 Rv0359 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_214873.1 437518 D 83332 CDS NP_214874.1 15607501 886480 complement(438302..438739) 1 NC_000962.3 Rv0360c, (MTCY13E10.22c), len: 145 aa. Conserved protein, equivalent to AL023514|MLCB4_16|CAA18948.1|AL023514|MLCB4.27c hypothetical protein from Mycobacterium leprae (137 aa),FASTA scores: opt: 793, E(): 0, (85.4% identity in 137 aa overlap). And similar to AL049754|SCH10_25c|T36537 hypothetical protein from Streptomyces coelicolor (143 aa),FASTA scores: opt: 497, E(): 3.2e-27, (55.8% identity in 138 aa overlap).; hypothetical protein 438739 886480 Rv0360c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214874.1 438302 R 83332 CDS NP_214875.1 15607502 886478 438822..439649 1 NC_000962.3 Rv0361, (MTCY13E10.23), len: 275 aa. Probable conserved membrane protein (has hydrophobic stretch from residues 132-156), equivalent to AL023514|MLCB4_17|AA18949.1|AL023514 putative membrane protein from Mycobacterium leprae (292 aa), FASTA scores: opt: 1044, E(): 0, (58.6% identity in 292 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved membrane protein 439649 886478 Rv0361 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214875.1 438822 D 83332 CDS NP_214876.1 15607503 886476 439871..441253 1 NC_000962.3 Rv0362, (MTCY13E10.24), len: 460 aa. Possible mgtE,magnesium (Mg2+) transport transmembrane protein; C-terminal region is highly similar to MGTE|G780283 putative Mg2+ transporter from Providencia stuarti (314 aa), FASTA scores: E(): 0, (47.2% identity in 307 aa overlap) (N-terminus extends approx. 150 aa further upstream compared to P. stuarti ORF). Also similar in part to others e.g. AAK20879.1|AF334760_1|AF334760 putative Mg2+ transporter from Aeromonas hydrophila (455 aa); NP_231292.1|NC_002505 magnesium transporter from Vibrio cholerae (451 aa); NP_102305.1|NC_002678 Mg2+ transport protein from Mesorhizobium loti (454 aa); etc. Also similar to Rv1232c|MTV006.04c from Mycobacterium tuberculosis (435 aa). Extended hydrophobic segment spanning last 130 residues. Belongs to the MgtE family.; Possible Mg2+ transport transmembrane protein MgtE 441253 mgtE 886476 mgtE Mycobacterium tuberculosis H37Rv Possible Mg2+ transport transmembrane protein MgtE NP_214876.1 439871 D 83332 CDS NP_214877.1 15607504 886474 complement(441265..442299) 1 NC_000962.3 Rv0363c, (MTCY13E10.25c), len: 344 aa. Probable fba (alternate gene name: fda), fructose bisphosphate aldolase , equivalent to AL023514|MLCB4_18|O69600|ALF_MYCLE fructose-bisphosphate aldolase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1995, E(): 0, (87.7% identity in 342 aa overlap). Also highly similar to others. Belongs to class II fructose-bisphosphate aldolase family. Cofactor: zinc.; Probable fructose-bisphosphate aldolase Fba 442299 fba 886474 fba Mycobacterium tuberculosis H37Rv Probable fructose-bisphosphate aldolase Fba NP_214877.1 441265 R 83332 CDS NP_214878.1 15607505 886473 442395..443078 1 NC_000962.3 Rv0364, (MTCY13E10.26), len: 227 aa. Possible conserved transmembrane protein, equivalent to O69601|Y364_MYCLE|ML0287|CAA18951.1|AL023514|AL023514|MLCB 4_19 hypothetical 24.3 KDA protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 1027, E(): 0, (66.1% identity in 227 aa overlap). Shows strong similarity to DEDA_ECOLI|P09548 DedA protein protein from Escherichia coli FASTA scores: E(): 1.3e-28, (39.5% identity in 195 aa overlap). Similar also to Mycobacterium tuberculosis DedA protein Rv2637|MTCY441.0.; Possible conserved transmembrane protein 443078 886473 Rv0364 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_214878.1 442395 D 83332 CDS NP_214879.1 15607506 886487 complement(443067..444197) 1 NC_000962.3 Rv0365c, (MTCY13E10.27c), len: 376 aa (start uncertain). Conserved protein (see citation below), very similar to G388212|CAA35191.1, a truncated ORF immediately upstream of the Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate aldolase (304 aa), FASTA scores: E(): 7.1e-19, (42.2% identity in 296 aa overlap).; hypothetical protein 444197 886487 Rv0365c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214879.1 443067 R 83332 CDS NP_214880.1 15607507 886471 complement(444222..444815) 1 NC_000962.3 Rv0366c, (MTV036.01c), len: 197 aa. Conserved hypothetical protein, showing weak similarity to HI1395|P44173|YD95_HAEIN hypothetical protein from Haemophilus influenzae (140 aa), FASTA scores: opt: 152,E(): 0.0015, (27.0% identity in 126 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00850 Glycine radical signature.; hypothetical protein 444815 886471 Rv0366c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214880.1 444222 R 83332 CDS NP_214881.1 15607508 886468 complement(444844..445233) 1 NC_000962.3 Rv0367c, (MTV036.02c), len: 129 aa. Hypothetical unknown protein.; Hypothetical protein 445233 886468 Rv0367c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214881.1 444844 R 83332 CDS NP_214882.1 15607509 886469 complement(445314..446525) 1 NC_000962.3 Rv0368c, (MTV036.03c), len: 403 aa. Conserved hypothetical protein, showing some similarity to AJ224684|BJAJ4684_4 cooxS protein from Bradyrhizobium japonicum (422 aa), FASTA scores: opt: 341, E(): 4.3e-13,(27.4% identity in 387 aa overlap); Rv2425c|MTCY428_22 hypothetical protein from Mycobacterium tuberculosis FASTA score: (30.7% identity in 238 aa overlap). Contains PS00213 Lipocalin signature.; hypothetical protein 446525 886469 Rv0368c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214882.1 445314 R 83332 CDS NP_214883.1 15607510 886485 complement(446531..447046) 1 NC_000962.3 Rv0369c, (MTV036.04c), len: 171 aa. Possible membrane protein oxidoreductase, similar to ORF 4 of the Pseudomonas thermocarboxydovorans protein of cutA-cutB-cutC gene cluster: X77931|PTC2CUTAC_4 ORF4 from Pseudomonas thermocarboxydovorans (171 aa), FASTA scores: opt: 226,E(): 9.8e-08, (31.3% identity in 166 aa overlap). Also similar to MTV036.05, MTV036.08, MTV036.09, and MTV026.10.; Possible membrane oxidoreductase 447046 886485 Rv0369c Mycobacterium tuberculosis H37Rv Possible membrane oxidoreductase NP_214883.1 446531 R 83332 CDS NP_214884.1 15607511 886465 complement(447147..448043) 1 NC_000962.3 Rv0370c, (MTV036.05c), len: 298 aa. Possible oxidoreductase, similar to many hypothetical proteins, but also similar to ORF4|X82447|OCCOXMSL4_4 Protein of coxMSL gene cluster from Pseudomonas/Oligotropha carboxidovorans (295 aa), FASTA scores: opt: 851, E(): 0, (48.2% identity in 282 aa overlap); AJ224684|BJAJ4684_3 cooxS from Bradyrhizobium japonicum (302 aa), FASTA scores: opt: 881,E(): 0, (47.6% identity in 290 aa overlap). Also highly similar to MTCY428_21 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible oxidoreductase 448043 886465 Rv0370c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_214884.1 447147 R 83332 CDS NP_214885.1 15607512 886463 complement(448040..448633) 1 NC_000962.3 Rv0371c, (MTV036.06c), len: 197 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL132824|SCAH10.09c|CAB60163.1|AL132824 hypothetical protein from Streptomyces coelicolor (207 aa),FASTA scores: opt: 247, E(): 4.5e-09, (32.3% identity in 195 aa overlap). Also weak similarity with YURE|D70017|Z99120|BSUB0017_134 hypothetical protein yurE from Bacillus subtilis (197 aa), FASTA scores: opt: 217,E(): 2.5e-08, (27.0% identity in 174 aa overlap).; hypothetical protein 448633 886463 Rv0371c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214885.1 448040 R 83332 CDS NP_214886.1 15607513 886460 complement(448630..449385) 1 NC_000962.3 Rv0372c, (MTV036.07c), len: 251 aa. Conserved hypothetical protein, showing some similarity with CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: opt: 186, E(): 1.6e-05, (41.1% identity in 95 aa overlap). Also similar to upstream ORF Rv0376c from Mycobacterium tuberculosis (380 aa), FASTA scores: E(): 6.8e-07, (31.0% identity in 277 aa overlap).; hypothetical protein 449385 886460 Rv0372c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214886.1 448630 R 83332 CDS NP_214887.1 15607514 886472 complement(449404..451803) 1 NC_000962.3 Rv0373c, (MTV036.08c), len: 799 aa. Probable carbon monoxide dehydrogenase, large chain, highly similar to others e.g. AAD00363.1| U80806|CUTL carbon monoxide dehydrogenase large subunit CutL protein from Hydrogenophaga pseudoflava (803 aa); S49124|509391|CAA54902.1|X77931|1094915|2107180C|CUTA carbon-monoxide dehydrogenase large chain (cut operon) from Pseudomonas thermocarboxydovorans (842 aa); C56279|809566|CAA57829.1|X82447|OCCOXMSL4_3|COXL carbon-monoxide dehydrogenase large chain (cluster coxMSL) from Pseudomonas/Oligotropha carboxydovorans (809 aa),FASTA scores: opt: 2484, E(): 0, (56.0% identity in 804 aa overlap); etc.; Probable carbon monoxyde dehydrogenase (large chain) 451803 886472 Rv0373c Mycobacterium tuberculosis H37Rv Probable carbon monoxyde dehydrogenase (large chain) NP_214887.1 449404 R 83332 CDS NP_214888.1 15607515 886462 complement(451800..452279) 1 NC_000962.3 Rv0374c, (MTV036.09c), len: 159 aa. Probable carbon monoxide dehydrogenase, small chain, highly similar to others e.g. B56279|5822285|X82447|OCCOXMSL4_2|COXS carbon-monoxide dehydrogenase small chain from Pseudomonas/Oligotropha carboxydovorans (166 aa), FASTA scores: opt: 662, E(): 0, (59.3% identity in 150 aa overlap); CAA12063.1|AJ224684 putative carbon monoxide dehydrogenase small subunit from Bradyrhizobium japonicum (161 aa); S49123|509390|CAA54901.1|X77931|CUTC carbon-monoxide dehydrogenase small chain from Pseudomonas thermocarboxydovorans (163 aa); etc.; Probable carbon monoxyde dehydrogenase (small chain) 452279 886462 Rv0374c Mycobacterium tuberculosis H37Rv Probable carbon monoxyde dehydrogenase (small chain) NP_214888.1 451800 R 83332 CDS NP_214889.1 15607516 886456 complement(452294..453154) 1 NC_000962.3 Rv0375c, (MTV036.10c), len: 286 aa. Probable carbon monoxide dehydrogenase, medium chain, similar to others e.g. AAD00361.1|U80806|CUTM carbon monoxide dehydrogenase middle subunit from Hydrogenophaga pseudoflava (287 aa); S49122|509389|CAA54900.1|X77931|CUTB carbon-monoxide dehydrogenase medium chain from Pseudomonas thermocarboxydovorans (287 aa); A56279|809564|CAA57827.1|X82447|OCCOXMSL4_1|COXM|CODH carbon-monoxide dehydrogenase medium chain from Pseudomonas/Oligotropha carboxydovorans (288 aa), FASTA scores: opt: 594, E(): 0, (37.5% identity in 277 aa overlap); etc.; Probable carbon monoxyde dehydrogenase (medium chain) 453154 886456 Rv0375c Mycobacterium tuberculosis H37Rv Probable carbon monoxyde dehydrogenase (medium chain) NP_214889.1 452294 R 83332 CDS NP_214890.1 15607517 886454 complement(453230..454372) 1 NC_000962.3 Rv0376c, (MTV036.11c), len: 380 aa. Conserved hypothetical protein, highly similar to T35481|4008539|CAA22508.1|AL034492|SC6C5.10 hypothetical protein from Streptomyces coelicolor (395 aa); and AAK64260.1|AF373840_20 ORF377 hypothetical CoxI from Arthrobacter nicotinovorans (377 aa). And similar to other conserved hypothetical proteins e.g. NP_101963.1|14021136|BAB47749.1|AP002994 hypothetical protein from Mesorhizobium loti (245 aa). Note that C-terminus shows similarity with C-termini of CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); CAB76250.1|X82447|COXI CoxI protein from Pseudomonas/Oligotropha carboxidovorans (330 aa); and AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: E(): 1.9e-17, (47.1% identity in 138 aa overlap). Also some partial similarity with AJ224684|BJAJ4684_5 cooxS from Bradyrhizobium japonicum (107 aa), FASTA scores: opt: 321, E(): 4.2e-14, (53.3% identity in 92 aa overlap); E1184330|Z99120|YURF YURF protein from Bacillus subtilis (330 aa), FASTA scores: opt: 170, E(): 2.9e- 16, (27.5% identity in 345 aa overlap). Also similar to downstream ORF Rv0372c from Mycobacterium tuberculosis (251 aa), FASTA scores: E(): 2.1e-06, (30.7% identity in 277 aa overlap).; hypothetical protein 454372 886454 Rv0376c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214890.1 453230 R 83332 CDS NP_214891.1 15607518 886452 454421..455386 1 NC_000962.3 Rv0377, (MTV036.12), len: 321 aa. Probable transcription regulator, lysR family, showing similarity with many hypothetical transcriptional regulators lysR homolog e.g. P32484|YEIE_ECOLI|M89774 hypothetical transcriptional regulator from Escherichia coli (293 aa),FASTA scores: opt: 265, E(): 4.9e-11, (28.6% identity in 266 aa overlap). Also similar to Rv2282c from Mycobacterium tuberculosis. Contains PS00044 bacterial regulatory protein lysR family signature. Seems to belong to the LysR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably LysR-family) 455386 886452 Rv0377 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably LysR-family) NP_214891.1 454421 D 83332 CDS NP_214892.1 15607519 886450 455637..455858 1 NC_000962.3 Rv0378, (MTV036.13), len: 73 aa. Conserved hypothetical gly-rich protein, showing some similarity to Mycobacterium tuberculosis PE_PGRS family; also similar to MTCY06H11_16|Z85982 hypothetical glycine-rich 88.5 KD protein (1011 aa), FASTA scores: opt: 237, E(): 0.0032,(58.7% identity in 63 aa overlap); MTV043_25.; Conserved hypothetical glycine rich protein 455858 886450 Rv0378 Mycobacterium tuberculosis H37Rv Conserved hypothetical glycine rich protein NP_214892.1 455637 D 83332 CDS YP_177722.1 57116727 886449 455977..456192 1 NC_000962.3 Rv0379, (MTV036.14), len: 71 aa. Possible secE2,protein transport protein, showing similarity with P27340|S61G_SULSO|SECE preprotein translocase SECE subunit (protein transport protein SEC61 gamma subunit homolog) from Sulfolobus acidocaldarius (65 aa), FASTA scores: opt: 79, E(): 4.7. (30.3% identity in 66 aa overlap); and hypothetical proteins e.g. Q9HPW4|VNG1446H hypothetical protein from Halobacterium sp. strain NRC-1 (77 aa); Q9I794|PA0038 hypothetical protein from Pseudomonas aeruginosa (71 aa); etc. Also highly similar to U85467|MTU85467_1 hypothetical Mycobacterium tuberculosis protein from a patient isolate (116 aa), FASTA scores: opt: 443, E(): 7.7e-29, (98.6% identity in 71 aa overlap). Note that for Rv0379|MTV036.14, a translation initiation region different to the one in U85467|MTU85467_1 was chosen. Could be a part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c,SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732.; Possible protein transport protein SecE2 456192 secE2 886449 secE2 Mycobacterium tuberculosis H37Rv Possible protein transport protein SecE2 YP_177722.1 455977 D 83332 CDS NP_214894.1 15607521 886446 complement(456268..456819) 1 NC_000962.3 Rv0380c, (MTV036.15c), len: 183 aa. Possible RNA methyltransferase, equivalent to CAC32002.1|AL583925 possible RNA methyltransferase from Mycobacterium leprae (182 aa). Also some similarity with others methyltransferases e.g. P19396|TRMH_ECOLI|78514|JV0043 tRNA (guanosine-2'-O-)-methyltransferase (tRNA methyltransferase) from Escherichia coli (229 aa), FASTA scores: opt: 227, E(): 1.4e-09, (28.9% identity in 166 aa overlap). Also similar to Rv0881, Rv3579c, Rv1644 from Mycobacterium tuberculosis.; Possible RNA methyltransferase (RNA methylase) 456819 886446 Rv0380c Mycobacterium tuberculosis H37Rv Possible RNA methyltransferase (RNA methylase) NP_214894.1 456268 R 83332 CDS NP_214895.1 15607522 886444 complement(456915..457823) 1 NC_000962.3 Rv0381c, (MTV036.16c), len: 302 aa. Hypothetical unknown protein. Equivalent to AAK44616.1 from Mycobacterium tuberculosis strain CDC1551 (254 aa) but longer 48 aa.; Hypothetical protein 457823 886444 Rv0381c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214895.1 456915 R 83332 CDS YP_177723.1 57116728 886443 complement(457841..458380) 1 NC_000962.3 Rv0382c, (MTV036.17c), len: 179 aa. Probable pyrE,orotate phosphoribosyltransferase, equivalent to CAC32004.1|AL583925 probable purine/pyrimidine phosphoribosyltransferase from Mycobacterium leprae (179 aa). Also highly similar to many others e.g. T36540|4753874|CAB42037.1|AL049754|SCH10.28c probable orotate phosphoribosyltransferase from Streptomyces coelicolor (182 aa); H69115|2622996|AAB86326.1|AE000938_10|MTH1860 probable orotate phosphoribosyltransferase from Methanobacterium thermoautotrophicum (180 aa), FASTA scores: opt: 389, E(): 2.7e-20, (40.7% identity in 172 aa overlap); O08359|PYRE_SULAC|2065444|CAA73352.1|Y12822 orotate phosphoribosyltransferase from Sulfolobus acidocaldarius (197 aa); etc. Note that also similar to other puridine 5'-monophosphate synthases (umpA genes; UMP synthases),generally in N-terminus that corresponds to orotate phosphoribosyltransferase activity. Contains PS00589 PTS HPR component serine phosphorylation site signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Note that previously known as umpA. Nucleotide position 458282 in the genome sequence has been corrected,A:G resulting in Y33Y.; Probable orotate phosphoribosyltransferase PyrE (OPRT) (oprtase) 458380 pyrE 886443 pyrE Mycobacterium tuberculosis H37Rv Probable orotate phosphoribosyltransferase PyrE (OPRT) (oprtase) YP_177723.1 457841 R 83332 CDS NP_214897.1 15607524 886458 complement(458461..459315) 1 NC_000962.3 Rv0383c, (MTV036.18c), len: 284 aa. Possible conserved secreted protein, with hydrophobic stretch in N-terminus and Pro-rich C-terminus. Equivalent to CAC32006.1|AL583925 possible secreted protein from Mycobacterium leprae (286 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Possible conserved secreted protein 459315 886458 Rv0383c Mycobacterium tuberculosis H37Rv Possible conserved secreted protein NP_214897.1 458461 R 83332 CDS NP_214898.1 15607525 886440 complement(459456..462002) 1 NC_000962.3 Rv0384c, (MTV036.19c), len: 848 aa. Probable clpB (alternate gene name: htpM), endopeptidase ATP-binding protein, chain B, equivalent to AC32007.1|AL583925 heat shock protein from Mycobacterium leprae (848 aa). Also highly similar to others e.g. P53532|CLPB_CORGL|1163118|AAB49540.1|U43536|CGU43536_1 CLPB protein (heat-inducible expression) from Corynebacterium glutamicum (852 aa), FASTA scores: opt: 4113, E(): 0,(74.5% identity in 846 aa overlap); T36551|4753885|CAB42048.1|AL049754|clpB|SCOEDB|SCH10.39c probable ATP-dependent proteinase ATP-binding chain from Streptomyces coelicolor (853 aa); P03815|CLPB_ECOLI|1788943|AAC75641.1|AE000345 CLPB protein (heat shock protein F84.1) from Escherichia coli strains K12 and O157:H7 (857 aa); etc. Also similar to Rv3596c|ClpC from Mycobacterium tuberculosis. Contains PS00870 and PS00871 Chaperonins clpA/B signatures and two PS000017 ATP/GTP-binding site motives a (P-loop). Belongs to the CLPA/CLPB family. Contains probable coiled-coil domain from aa 411-503. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1) 462002 clpB 886440 clpB Mycobacterium tuberculosis H37Rv Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1) NP_214898.1 459456 R 83332 CDS NP_214899.1 15607526 886441 462135..463307 1 NC_000962.3 Rv0385, (MTV036.20), len: 390 aa. Probable monooxygenase, similar to T37003|5738846|CAB52917.1|AL109949 probable flavohemoprotein from Streptomyces coelicolor (435 aa); and similar in part (C-termini) to various monooxygenases e.g. P19734|DMPP_PSESP|94993|F37831 phenol hydroxylase P5 protein (phenol 2-monooxygenase P5 component) from Pseudomonas putida (353 aa), FASTA scores: opt: 363, E(): 4.2e-16, (31.8% identity in 255 aa overlap); S47292|2120861|pir|S70085 phenol 2-monooxygenase chain mopP from Acinetobacter calcoaceticus (350 aa); P21394|XYLA_PSEPU|94933|B37316 xylene monooxygenase electron transfer component [includes: ferredoxin; ferredoxin--NAD(+) reductase] from Pseudomonas putida plasmid pWW0 (350 aa); AAC38360.1|AF043544|NtnMA|ntnA reductase component of 4-nitrotoluene monooxygenase from Pseudomonas sp. (328 aa); etc.; Probable monooxygenase 463307 886441 Rv0385 Mycobacterium tuberculosis H37Rv Probable monooxygenase NP_214899.1 462135 D 83332 CDS NP_214900.1 15607527 886030 463411..466668 1 NC_000962.3 Rv0386, (MTV036.21), len: 1085 aa. Probable regulatory protein, LuxR/uhpA family, highly similar to CAC30706.1|AL583923 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar in part to other regulatory proteins e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); N-terminus of CAB92369.1|AL356612 putative AfsR-like regulatory protein from Streptomyces coelicolor (1114 aa); N-terminus of NP_107139.1|14026327|BAB52925.1|AP003009 transcriptional regulator from Mesorhizobium loti (952 aa); AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 224, E() : 1.1e-06,(26.1% identity in 867 aa overlap); etc. Also similar to many putative Mycobacterium tuberculosis regulatory proteins e.g. AL0212|MTV008_44 (1137 aa), FASTA scores: opt: 3756, E(): 0, (56.7% identity in 1089 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop),PS00622 Bacterial regulatory proteins, luxR family signature and probable helix-turn-helix motif at aa 1042-1063 (Score 1025, +2.68 S D). Belongs to the LuxR/UhpA family of transcriptional regulators.; Probable transcriptional regulatory protein (probably LuxR/UhpA-family) 466668 886030 Rv0386 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably LuxR/UhpA-family) NP_214900.1 463411 D 83332 CDS NP_214901.1 15607528 886436 complement(466672..467406) 1 NC_000962.3 Rv0387c, (MTV036.22c), len: 244 aa. Conserved hypothetical protein, showing some similarity to MTCI237.20c, and M17282|HUMEL20_1 Human elastin gene, exon 1, Elastin (687 aa), FASTA scores: opt: 193, E(): 0.35,(34.4% identity in 189 aa overlap).; hypothetical protein 467406 886436 Rv0387c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214901.1 466672 R 83332 CDS YP_177724.1 57116729 886439 complement(467459..468001) 1 NC_000962.3 Rv0388c, (MTV036.23c), len: 180 aa. PPE9, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. MTCY10G2_10|Z92539 from Mycobacterium tuberculosis (391 aa), FASTA scores: opt: 667, E(): 0,(58.3% identity in 180 aa overlap) but much shorter.; PPE family protein PPE9 468001 PPE9 886439 PPE9 Mycobacterium tuberculosis H37Rv PPE family protein PPE9 YP_177724.1 467459 R 83332 CDS NP_214903.1 15607530 886032 468335..469594 1 NC_000962.3 Rv0389, (MTCY04D9.01, MTV036.24), len: 419 aa. Probable purT, phosphoribosylglycinamide formyltransferase 2, similar to others e.g. P33221|PURT_ECOLI|B1849 phosphoribosylglycinamide formyltransferase 2 from Escherichia coli strain K-12 (391 aa), FASTA scores: opt: 481, E(): 1.3e-22, (40.1% identity in 379 aa overlap); etc. Belongs to the PurK / PurT family. Cofactor: magnesium.; Probable phosphoribosylglycinamide formyltransferase 2 PurT (GART 2) (gar transformylase 2) (5'-phosphoribosylglycinamide transformylase 2) (formate-dependent gar transformylase) 469594 purT 886032 purT Mycobacterium tuberculosis H37Rv Probable phosphoribosylglycinamide formyltransferase 2 PurT (GART 2) (gar transformylase 2) (5'-phosphoribosylglycinamide transformylase 2) (formate-dependent gar transformylase) NP_214903.1 468335 D 83332 CDS NP_214904.1 15607531 886433 469591..470013 1 NC_000962.3 Rv0390, (MTCY04D9.02), len: 140 aa. Conserved protein, equivalent to AL023514|MLCB4_11|CAA18942.1|AL023514 hypothetical protein from Mycobacterium leprae (147 aa), FASTA scores: opt: 778,E(): 0, (79.0% identity in 138 aa overlap). Also similar to hypothetical proteins from several Rickettsia species.; hypothetical protein 470013 886433 Rv0390 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214904.1 469591 D 83332 CDS NP_214905.1 15607532 886431 470010..471230 1 NC_000962.3 Rv0391, (MTCY04D9.03), len: 406 aa. Probable metZ,O-succinylhomoserine sulfhydrylase, equivalent, but shorter 20 aa in N-terminus, to AA18941.1|AL023514 O-succinylhomoserine sulfhydrylase from Mycobacterium leprae (426 aa). Also highly similar to others e.g. METZ_PSEAE|P55218 o-succinylhomoserine sulfhydrylase from Pseudomonas aeruginosa (403 aa), FASTA scores: opt: 1175,E(): 0, (47.2% identity in 392 aa overlap); etc. Belongs to the trans-sulfuration enzymes family. Could also be a cystathionine gamma-synthase.; Probable O-succinylhomoserine sulfhydrylase MetZ (OSH sulfhydrylase) 471230 metZ 886431 metZ Mycobacterium tuberculosis H37Rv Probable O-succinylhomoserine sulfhydrylase MetZ (OSH sulfhydrylase) NP_214905.1 470010 D 83332 CDS NP_214906.1 15607533 886430 complement(471227..472639) 1 NC_000962.3 Rv0392c, (MTCY04D9.04c), len: 470 aa. Probable ndhA,membrane NADH dehydrogenase, equivalent to many e.g. AF038423|AF038423_1 NADH dehydrogenase from Mycobacterium smegmatis (457 aa), FASTA scores: opt: 1991, E(): 0, (67.9% identity in 458 aa overlap); MLCB1788_3 NADH dehydrogenase from Mycobacterium leprae (466 aa), FASTA score: (62.5% identity in 467 aa overlap). Also similar to others from several organisms e.g. P00393|DHNA_ECOLI|66211|581140|CAA23586.1|V00306 NADH dehydrogenase from Escherichia coli (434 aa); and Rv0392c|ndhB from Mycobacterium tuberculosis. Has hydrophobic stretch in C-terminus. Belongs to the NADH dehydrogenase family.; Probable membrane NADH dehydrogenase NdhA 472639 ndhA 886430 ndhA Mycobacterium tuberculosis H37Rv Probable membrane NADH dehydrogenase NdhA NP_214906.1 471227 R 83332 CDS NP_214907.1 15607534 886428 472781..474106 1 NC_000962.3 Rv0393, (MTCY04D9.05), len: 441 aa. Member of Mycobacterium tuberculosis 13E12 repeat family of conserved proteins, similar to many e.g. Rv1148c, Rv1945, Rv3467,Rv0336|MTCY279_3 (503 aa), FASTA scores: E(): 0, (61.1% identity in 347 aa overlap).; Conserved 13E12 repeat family protein 474106 886428 Rv0393 Mycobacterium tuberculosis H37Rv Conserved 13E12 repeat family protein NP_214907.1 472781 D 83332 CDS NP_214908.1 15607535 886435 complement(474122..474841) 1 NC_000962.3 Rv0394c, (MTCY04D9.06c), len: 239 aa. Possible secreted protein, sharing no homology with other proteins. Has hydrophobic stretch at its N-terminus.; Possible secreted protein 474841 886435 Rv0394c Mycobacterium tuberculosis H37Rv Possible secreted protein NP_214908.1 474122 R 83332 CDS NP_214909.1 15607536 886425 474940..475344 1 NC_000962.3 Rv0395, (MTCY04D9.07), len: 134 aa. Hypothetical unknown protein.; Hypothetical protein 475344 886425 Rv0395 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214909.1 474940 D 83332 CDS NP_214910.1 15607537 886423 475350..475742 1 NC_000962.3 Rv0396, (MTCY04D9.08), len: 130 aa. Hypothetical unknown protein.; Hypothetical protein 475742 886423 Rv0396 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214910.1 475350 D 83332 CDS NP_214911.1 15607538 886421 475816..476184 1 NC_000962.3 Rv0397, (MTCY04D9.09), len: 122 aa. Part of 13E12 repeat family of conserved Mycobacterium tuberculosis proteins, similar to downstream Rv0393|Z84725|MTCY4D9_5 conserved 13E12 repeat family protein (441 aa), FASTA scores: E(): 0, (87.7% identity in 122 aa overlap).; Conserved 13E12 repeat family protein 476184 886421 Rv0397 Mycobacterium tuberculosis H37Rv Conserved 13E12 repeat family protein NP_214911.1 475816 D 83332 CDS YP_004837048.2 448824743 14515846 476394..476642 1 NC_000962.3 Rv0397A, len: 82 aa. Conserved protein.; hypothetical protein 476642 14515846 Rv0397A Mycobacterium tuberculosis H37Rv hypothetical protein YP_004837048.2 476394 D 83332 CDS NP_214912.1 15607539 886419 complement(476679..477320) 1 NC_000962.3 Rv0398c, (MTCY04D9.10c), len: 213 aa. Possible secreted protein, sharing no homology with other proteins. Has potential signal sequence with hydrophobic stretch from aa 7-25.; Possible secreted protein 477320 886419 Rv0398c Mycobacterium tuberculosis H37Rv Possible secreted protein NP_214912.1 476679 R 83332 CDS NP_214913.1 15607540 886416 complement(477327..478556) 1 NC_000962.3 Rv0399c, (MTCY04D9.11c), len: 409 aa. Possible lpqK,conserved lipoprotein, showing some similarity to penicillin binding proteins and various peptidases e.g. DAC_STRSQ|P15555 d-alanyl-d-alanine carboxypeptidase protein (406 aa), FASTA scores: opt: 348, E(): 5.6e-16,(29.2% identity in 301 aa overlap). Also similar to other Mycobacterium tuberculosis PBPs and esterases. Has possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013).; Possible conserved lipoprotein LpqK 478556 lpqK 886416 lpqK Mycobacterium tuberculosis H37Rv Possible conserved lipoprotein LpqK NP_214913.1 477327 R 83332 CDS NP_214914.1 15607541 886427 complement(478566..479753) 1 NC_000962.3 Rv0400c, (MTCY04D9.12c), len: 395 aa. Probable fadE7, acyl-CoA dehydrogenase, similar to many e.g. CAC12923.1|AL445403 putative acyl CoA dehydrogenase from Streptomyces coelicolor (397 aa); G624219 glutaryl-CoA dehydrogenase precursor (438 aa), FASTA scores: opt: 1161,E(): 0, (48.1% identity in 391 aa overlap); etc.; Acyl-CoA dehydrogenase FadE7 479753 fadE7 886427 fadE7 Mycobacterium tuberculosis H37Rv Acyl-CoA dehydrogenase FadE7 NP_214914.1 478566 R 83332 CDS NP_214915.1 15607542 886438 479789..480160 1 NC_000962.3 Rv0401, (MTCY04D9.14), len: 123 aa. Probable conserved transmembrane protein, equivalent to AL023514|MLCB4_9 putative integral membrane protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 548, E(): 4.4e-32, (66.9% identity in 121 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 480160 886438 Rv0401 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_214915.1 479789 D 83332 CDS NP_214916.1 15607543 886413 complement(480355..483231) 1 NC_000962.3 Rv0402c, (MTCY04D9.15c), len: 958 aa. Probable mmpL1, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCTU|Q11171 hypothetical 106.2 kDa membrane protein from Mycobacterium tuberculosis (968 aa), FASTA scores: opt: 3551, E(): 0, (55.4% identity in 933aa overlap); YV34_MYCLE|P54881 hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 3615,E(): 0, (55.5% identity in 941 aa overlap); etc. Highly similar to many other mycobacterial MmpL proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. Rv0450c, Rv0676c, Rv0507, etc. Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL1 483231 mmpL1 886413 mmpL1 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL1 NP_214916.1 480355 R 83332 CDS NP_214917.1 15607544 886411 complement(483228..483656) 1 NC_000962.3 Rv0403c, (MTCY04D9.16c), len: 142 aa. Probable mmpS1, conserved membrane protein (see citation below),highly similar to other Mycobacterial proteins e.g. YV33_MYCLE|P54880 hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 458, E(): 1.6e-26, (46.9% identity in 143 aa overlap); YV33_MYCTU|Q11170 hypothetical 15.9 kDa protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 362, E(): 1.1e-19, (42.1% identity in 140 aa overlap); etc. Also similar to other MmpS proteins from Mycobacterium tuberculosis e.g. Rv0677c, Rv0451c, etc. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein MmpS1 483656 mmpS1 886411 mmpS1 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein MmpS1 NP_214917.1 483228 R 83332 CDS NP_214918.1 15607545 886409 483977..485734 1 NC_000962.3 Rv0404, (MTCY04D9.17-MTCY22G10.00), len: 585 aa. fadD30, fatty-acid-AMP synthetase, similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa); MASC_MYCLE|P54200 masc protein from Mycobacterium leprae (372 aa), FASTA scores: opt: 888, E(): 0, (44.2% identity in 342 aa overlap). Also similar to Y06J_MYCTU|Q10976 hypothetical 67.9 kDa protein (626 aa), FASTA scores: opt: 1463, E(): 0, (42.4% identity in 568 aa overlap).; Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 485734 fadD30 886409 fadD30 Mycobacterium tuberculosis H37Rv Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_214918.1 483977 D 83332 CDS NP_214919.1 15607546 886407 485731..489939 1 NC_000962.3 Rv0405, (MTCY22G10.01), len: 1402 aa. Probable pks6,membrane-bound polyketide synthase (see citation below),highly similar to others e.g. CAC29643.1|AL583917 putative polyketide synthase from Mycobacterium leprae (2103 aa); Y06K_MYCTU|Q10977 probable polyketide synthase (1876 aa),FASTA scores: opt: 2303, E(): 0, (38.7% identity in 1232 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site, 2 x PS00017 ATP/GTP-binding site motif A (P-loop), and PS00012 Phosphopantetheine attachment site.; Probable membrane bound polyketide synthase Pks6 489939 pks6 886407 pks6 Mycobacterium tuberculosis H37Rv Probable membrane bound polyketide synthase Pks6 NP_214919.1 485731 D 83332 CDS NP_214920.1 15607547 886403 complement(489887..490705) 1 NC_000962.3 Rv0406c, (MTCY22G10.02c), len: 272 aa. Beta-lactamase-like protein, equivalent to AAD38170.1|AF152397_1 beta-lactamase-like protein from Mycobacterium phlei (243 aa); AL023514|MLCB4_8 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1284, E(): 0, (74.9% identity in 243 aa overlap); and AAD38164.1|AF152394_2 beta-lactamase-like protein from Mycobacterium avium (247 aa), FASTA scores: opt: 1301, E(): 0, (74.2% identity in 244 aa overlap); etc. Also slight similarity to others beta-lactamases and hypothetical proteins e.g. P52700|BLA1_XANMA|628530|S45349 metallo-beta-lactamase L1 precursor (beta-lactamase, type II) (penicillinase) from Xanthomonas maltophilia (290 aa),FASTA scores: (34.4% identity in 96 aa overlap). Recombinant protein has beta lactamase activity (See Nampoothiri et al., 2008).; Beta lactamase like protein 490705 886403 Rv0406c Mycobacterium tuberculosis H37Rv Beta lactamase like protein NP_214920.1 489887 R 83332 CDS NP_214921.1 15607548 886418 490783..491793 1 NC_000962.3 Rv0407, (MTCY22G10.03), len: 336 aa. fgd1,F420-dependent glucose-6-phosphate dehydrogenase,equivalent to others from Mycobacteria e.g. AAD38165.1|AF152394_3 from Mycobacterium avium (336 aa),FASTA scores: opt: 2082, E(): 0, (89.9% identity in 336 aa overlap); AL023514|MLCB 4_7 from Mycobacterium leprae (336 aa), FASTA scores: opt: 2069, E(): 0, (89.0% identity in 336 aa overlap). Also similar to other dehydrogenases e.g. CAA77276.1|Y18730 F420-dependent alcohol dehydrogenase from Methanofollis liminatans (330 aa). Also similar to many proteins from Mycobacterium tuberculosis e.g. Rv0953c,Rv0791c, etc. Note that previously known as fgd.; F420-dependent glucose-6-phosphate dehydrogenase Fgd1 491793 fgd1 886418 fgd1 Mycobacterium tuberculosis H37Rv F420-dependent glucose-6-phosphate dehydrogenase Fgd1 NP_214921.1 490783 D 83332 CDS NP_214922.1 15607549 886401 491786..493858 1 NC_000962.3 Rv0408, (MTCY22G10.04), len: 690 aa. Probable pta,phosphate acetyltransferase, highly similar to others e.g. PTA_ECOLI|P39184|11279789|JX0357|B2297 phosphate acetyltransferase from Escherichia coli strain K12 (713 aa), FASTA scores: opt: 1303, E(): 0, (38.0% identity in 718 aa overlap); etc. Belongs to the phosphate acetyltransferase and butyryltransferase family.; Probable phosphate acetyltransferase Pta (phosphotransacetylase) 493858 pta 886401 pta Mycobacterium tuberculosis H37Rv Probable phosphate acetyltransferase Pta (phosphotransacetylase) NP_214922.1 491786 D 83332 CDS NP_214923.1 15607550 886399 493851..495008 1 NC_000962.3 Rv0409, (MTCY22G10.05), len: 385 aa. Probable ackA,acetate kinase, highly similar to others e.g. ACKA_BACSU|P37877 acetate kinase from Bacillus subtilis (395 aa), FASTA scores: opt: 974, E(): 0, (43.5% identity in 393 aa overlap); etc. Contains PS01075 Acetate and butyrate kinases family signature 1, PS00758 ArgE / dapE / ACY1/ CPG2 / yscS family signature 1. Belongs to the acetokinase family.; Probable acetate kinase AckA (acetokinase) 495008 ackA 886399 ackA Mycobacterium tuberculosis H37Rv Probable acetate kinase AckA (acetokinase) NP_214923.1 493851 D 83332 CDS NP_214924.1 15607551 886397 complement(495062..497314) 1 NC_000962.3 Rv0410c, (MTCY22G10.06c), len: 750 aa. PknG,serine/threonine-protein kinase (see citations below),equivalent to PKNG_MYCLE|P57993|13092623|CAC29812.1|AL583918 probable serine/threonine-protein kinase from Mycobacterium leprae (767 aa). Also similar to others e.g. AB76890.1|AL159139 putative serine/threonine protein kinase from Streptomyces coelicolor (774 aa); etc. Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Structure of PknG with inhibitor AX20017 reveals that the inhibitor-binding pocket is shaped by a unique set of amino acid side chains not found in any human kinase (See Scherr et al., 2007).; Serine/threonine-protein kinase PknG (protein kinase G) (STPK G) 497314 pknG 886397 pknG Mycobacterium tuberculosis H37Rv Serine/threonine-protein kinase PknG (protein kinase G) (STPK G) NP_214924.1 495062 R 83332 CDS NP_214925.1 15607552 886393 complement(497314..498300) 1 NC_000962.3 Rv0411c, (MTCY22G10.07c), len: 328 aa. Probable glnH, glutamine-binding protein, membrane-bound lipoprotein (see citation below), equivalent to AL035159|MLCB1450_15|T44736|4154051|CAA22704.1 glutamine-binding protein homolog from Mycobacterium leprae (325 aa), FASTA scores: opt: 1747, E(): 0, (79.3% identity in 328 aa overlap). Also similar to others e.g. GLNH_BACST|P27676 glutamine-binding protein precursor from Bacillus stearothermophilus (262 aa), FASTA scores: opt: 493, E(): 7.5e-22, (37.8% identity in 193 aa overlap); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS01039 Bacterial extracellular solute-binding proteins, family 3 signature. Belongs to the bacterial extracellular solute-binding protein family 3. Presumed attached to the membrane by a lipid anchor.; Probable glutamine-binding lipoprotein GlnH (GLNBP) 498300 glnH 886393 glnH Mycobacterium tuberculosis H37Rv Probable glutamine-binding lipoprotein GlnH (GLNBP) NP_214925.1 497314 R 83332 CDS NP_214926.1 15607553 886415 complement(498300..499619) 1 NC_000962.3 Rv0412c, (MTCY22G10.08c), len: 439 aa. Possible conserved membrane protein, equivalent to AL035159|MLCB1450_16|T44737 probable membrane protein from Mycobacterium leprae (403 aa), FASTA scores: opt: 2027,E(): 0, (80.4% identity in 403 aa overlap). Also some similarity with CAB71201.1|AL138538 putative secreted protein from Streptomyces coelicolor (429 aa).; Possible conserved membrane protein 499619 886415 Rv0412c Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_214926.1 498300 R 83332 CDS NP_214927.1 15607554 886405 499713..500366 1 NC_000962.3 Rv0413, (MTCY22G10.10), len: 217 aa. Possible mutT3,mutator protein (see citation below), showing some similarity with e.g. MUTT_PROVU|P32090 mutator mutt protein from Proteus vulgaris (112 aa), FASTA scores: opt: 151,E(): 0.0008, (40.7% identity in 59 aa overlap). Seems to belong to the NUDIX hydrolase family.; Possible mutator protein MutT3 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) (dGTP pyrophosphohydrolase) 500366 mutT3 886405 mutT3 Mycobacterium tuberculosis H37Rv Possible mutator protein MutT3 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) (dGTP pyrophosphohydrolase) NP_214927.1 499713 D 83332 CDS NP_214928.1 15607555 886391 complement(500350..501018) 1 NC_000962.3 Rv0414c, (MTCY22G10.11c), len: 222 aa. thiE, thiamin phosphate pyrophosphorylase, equivalent to Q9ZBL5|AL035159|MLCB1450_17 probable thiamine-phosphate pyrophosphorylase from Mycobacterium leprae (235 aa), FASTA scores: opt: 1095, E(): 0, (78.0% identity in 223 aa overlap). Also similar to others e.g. T34974|5689976|CAB52013.1|AL109663 probable thiamin phosphate pyrophosphorylase from Streptomyces coelicolor (223 aa); THIE_ECOLI|P30137 thie protein from Escherichia coli strain K12 (211 aa), FASTA scores: opt: 275, E(): 7.8e-12, (37.8% identity in 196 aa overlap); etc. Belongs to the TMP-PPASE family.; Thiamine-phosphate pyrophosphorylase ThiE (TMP pyrophosphorylase) (TMP-PPASE) (thiamine-phosphate synthase) 501018 thiE 886391 thiE Mycobacterium tuberculosis H37Rv Thiamine-phosphate pyrophosphorylase ThiE (TMP pyrophosphorylase) (TMP-PPASE) (thiamine-phosphate synthase) NP_214928.1 500350 R 83332 CDS NP_214929.1 15607556 886390 501148..502170 1 NC_000962.3 Rv0415, (MTCY22G10.12), len: 340 aa. Possible thiO,thiamine biosynthesis oxidoreductase, equivalent to T44739|4154054|CAA22708.1|AL035159|MLCB1450.24 hypothetical protein from Mycobacterium leprae (340 aa), FASTA scores: opt: 1867, E(): 0, (82.0% identity in 338 aa overlap). Shows some similarity to other thiO proteins e.g. THIO_RHIET|O34292 Putative thiamine biosynthesis oxidoreductase from Rhizobium etli plasmid pb (327 aa) (see citation below); AAG31046.1|AF264948_8|THIO putative amino acid oxidase flavoprotein ThiO from Erwinia amylovora (349 aa); NP_106392.1|14025578|BAB52178.1|AP003007|THIO thiamine biosynthesis oxidoreductase THIO from Mesorhizobium loti (333 aa); etc.; Possible thiamine biosynthesis oxidoreductase ThiO 502170 thiO 886390 thiO Mycobacterium tuberculosis H37Rv Possible thiamine biosynthesis oxidoreductase ThiO NP_214929.1 501148 D 83332 CDS NP_214930.1 15607557 886395 502167..502373 1 NC_000962.3 Rv0416, (MTCY22G10.13), len: 68 aa. Possible thiS protein, equivalent to T44740|4154055|CAA22709.1|AL035159|MLCB1450.25 hypothetical protein from Mycobacterium leprae (74 aa), FASTA scores: opt: 303, E(): 2e-18, (71.6% identity in 74 aa overlap). Shows weak similarity with O32583|THIS_ECOLI|THIG1|B3991.1 this protein from Escherichia coli strain K12 (66 aa),FASTA scores: opt: 103, E(): 0.052, (30.9% identity in 68 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible protein ThiS 502373 thiS 886395 thiS Mycobacterium tuberculosis H37Rv Possible protein ThiS NP_214930.1 502167 D 83332 CDS NP_214931.1 15607558 886396 502366..503124 1 NC_000962.3 Rv0417, (MTCY22G10.14), len: 252 aa. Probable thiG,thiamin biosynthesis protein, equivalent to AL035159|MLCB1450_20|T44741|THIG probable thiamin biosynthesis protein from Mycobacterium leprae (261 aa),FASTA scores: opt: 1380, E(): 0, (86.8% identity in 250 aa overlap). Also highly similar to others e.g. SCOEDB|SC6E10.03|T35490|THIG probable thiazole biosynthesis protein from Streptomyces coelicolor (264 aa); F82761|9105679|AAF83593.1|AE003919_4|XF0783|THIG thiamin biosynthesis protein thiG from Xylella fastidiosa (275 aa); P30139|THIG_ECOLI|7448315|B65206|409790|AAC43089.1|U00006 THIG protein thiamin biosynthesis protein from Escherichia coli strain K-12 (281 aa); etc. Belongs to the THIG family.; Probable thiamin biosynthesis protein ThiG (thiazole biosynthesis protein) 503124 thiG 886396 thiG Mycobacterium tuberculosis H37Rv Probable thiamin biosynthesis protein ThiG (thiazole biosynthesis protein) NP_214931.1 502366 D 83332 CDS NP_214932.1 15607559 886381 503496..504998 1 NC_000962.3 Rv0418, (MTCCY22G10.15), len: 500 aa. Probable lpqL,lipoprotein aminopeptidase, similar to others e.g. B83278|9949035|AAG06327.1|AE004720_3|AE004720|PA2939 probable aminopeptidase from Pseudomonas aeruginosa (536 aa); P80561|APX_STRGR|SGAP|S66427 aminopeptidase from Streptomyces griseus (284 aa) (homology only with C-terminus of Rv0418); P37302|APE3_YEAST|1077010|A54134 aminopeptidase Y from Saccharomyces cerevisiae (537 aa); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein aminopeptidase LpqL 504998 lpqL 886381 lpqL Mycobacterium tuberculosis H37Rv Probable lipoprotein aminopeptidase LpqL NP_214932.1 503496 D 83332 CDS NP_214933.1 15607560 886388 505086..506582 1 NC_000962.3 Rv0419, (MTCY22G10.16), len: 498 aa. Possible lpqM,lipoprotein peptidase ; has potential N-terminal signal peptide and contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS00142 Neutral zinc metallopeptidases, zinc-binding region signature.; Possible lipoprotein peptidase LpqM 506582 lpqM 886388 lpqM Mycobacterium tuberculosis H37Rv Possible lipoprotein peptidase LpqM NP_214933.1 505086 D 83332 CDS NP_214934.1 15607561 886383 complement(506561..506971) 1 NC_000962.3 Rv0420c, (MTCY22G10.17c), len: 136 aa. Possible transmembrane protein; has potential transmembrane domains aa 53-99 and aa 100-122.; Possible transmembrane protein 506971 886383 Rv0420c Mycobacterium tuberculosis H37Rv Possible transmembrane protein NP_214934.1 506561 R 83332 CDS NP_214935.1 15607562 886377 complement(507132..507761) 1 NC_000962.3 Rv0421c, (MTCY22G10.18c), len: 209 aa. Conserved hypothetical protein, showing similarity with NP_103507.1|14022684|BAB49293.1|AP002998 hypothetical protein from Mesorhizobium loti (214 aa).; hypothetical protein 507761 886377 Rv0421c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214935.1 507132 R 83332 CDS NP_214936.1 15607563 886375 complement(507758..508555) 1 NC_000962.3 Rv0422c, (MTCY22G10.19c), len: 265 aa. Probable thiD, phosphomethylpyrimidine kinase, equivalent to AL035159|MLCB1450_21 phosphomethylpyrimidine kinase from Mycobacterium leprae (279 aa), FASTA scores: opt: 1386,E(): 0, (77.8% identity in 266 aa overlap). Also highly similar to others e.g. HIU32725_3|P44697|THID_HAEIN phosphomethylpyrimidine kinase from Haemophilus influenzae (269 aa), FASTA scores: opt: 605, E(): 0, (42.1% identity in 259 aa overlap). Belongs to the ThiD family.; Probable phosphomethylpyrimidine kinase ThiD (HMP-phosphate kinase) (HMP-P kinase) 508555 thiD 886375 thiD Mycobacterium tuberculosis H37Rv Probable phosphomethylpyrimidine kinase ThiD (HMP-phosphate kinase) (HMP-P kinase) NP_214936.1 507758 R 83332 CDS NP_214937.1 15607564 886379 complement(508582..510225) 1 NC_000962.3 Rv0423c, (MTCY22G10.20c), len: 547 aa. Probable thiC, thiamin biosynthesis protein, equivalent to Q9ZBL0|THIC_MYCLE|11279601|T44743|AL035159|MLCB1450_22 thiamine biosynthesis protein from Mycobacterium leprae (547 aa), FASTA scores: opt: 3283, E(): 0, (90.1% identity in 547 aa overlap). Also highly similar to others e.g. P45740|THIC_BACSU thiamin biosynthesis protein from Bacillus subtilis (590 aa), FASTA scores: opt: 2295, E(): 0, (65.2% identity in 580 aa overlap); P30136|THIC_ECOLI THIC protein from Escherichia coli strain K12 (631 aa),FASTA scores: opt: 2141, E(): 0, (62.1% identity in 568 aa overlap); etc. Belongs to the ThiC family.; Probable thiamine biosynthesis protein ThiC 510225 thiC 886379 thiC Mycobacterium tuberculosis H37Rv Probable thiamine biosynthesis protein ThiC NP_214937.1 508582 R 83332 CDS NP_214938.1 15607565 886374 complement(510377..510652) 1 NC_000962.3 Rv0424c, (MTCY22G10.21c), len: 91 aa. Hypothetical unknown protein.; Hypothetical protein 510652 886374 Rv0424c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214938.1 510377 R 83332 CDS NP_214939.1 15607566 886373 complement(510702..515321) 1 NC_000962.3 Rv0425c, (MTCY22G10.22c), len: 1539 aa. Possible ctpH, metal cation-transporting P-type ATPase (transmembrane protein), showing some similarity with CAA17934.1|AL022118|13093871|CAC32203.1|AL583926 putative cation-transporting ATPase from Mycobacterium leprae (1609 aa). Also similar to others ATPases e.g. AE000873_1 cation-transporting P-ATPase from Methanobacterium thermoautotrop (844 aa), FASTA score: (30.5% identity in 827 aa overlap); AB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); etc. C-terminal region similar to other ATPases from Mycobacterium tuberculosis e.g. Y05Q_MYCTU|Q10900 putative cation-transporting ATPase C (855 aa), FASTA scores: opt: 770, E(): 5.3e-32, (44.9% identity in 820 aa overlap). Nucleotide position 511518 in the genome sequence has been corrected, T:G resulting in I1268I.; Possible metal cation transporting P-type ATPase CtpH 515321 ctpH 886373 ctpH Mycobacterium tuberculosis H37Rv Possible metal cation transporting P-type ATPase CtpH NP_214939.1 510702 R 83332 CDS NP_214940.1 15607567 886387 complement(515373..515816) 1 NC_000962.3 Rv0426c, (MTCY22G10.23c), len: 147 aa. Possible transmembrane protein; has potential transmembrane domains aa 19-41, and aa 61-83.; Possible transmembrane protein 515816 886387 Rv0426c Mycobacterium tuberculosis H37Rv Possible transmembrane protein NP_214940.1 515373 R 83332 CDS NP_214941.1 15607568 886370 complement(516017..516892) 1 NC_000962.3 Rv0427c, (MTCY22G10.24c), len: 291 aa. Probable xthA (alternate gene name: xth), exodeoxyribonuclease III protein (see citation below), similar to others e.g. EX3_ECOLI|P09030 exodeoxyribonuclease III from Escherichia Coli strain K12 (268 aa), FASTA scores: opt: 360, E(): 1.2e-17, (29.3% identity in 270 aa overlap); etc. Belongs to the AP/EXOA family of DNA repair enzymes.; Probable exodeoxyribonuclease III protein XthA (exonuclease III) (EXO III) (AP endonuclease VI) 516892 xthA 886370 xthA Mycobacterium tuberculosis H37Rv Probable exodeoxyribonuclease III protein XthA (exonuclease III) (EXO III) (AP endonuclease VI) NP_214941.1 516017 R 83332 CDS NP_214942.1 15607569 886368 complement(516895..517803) 1 NC_000962.3 Rv0428c, (MTCY22G10.25c), len: 302 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005.; GCN5-related N-acetyltransferase 517803 886368 Rv0428c Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_214942.1 516895 R 83332 CDS NP_214943.1 15607570 886366 complement(517803..518396) 1 NC_000962.3 Rv0429c, (MTCY22G10.26c), len: 197 aa. Probable def,polypeptide deformylase, equivalent to CAC30884.1|AL583923 polypeptide deformylase from Mycobacterium leprae (197 aa). Also similar to others e.g. DEF_ECOLI|P27251|95874|S23107 polypeptide deformylase from Escherichia coli (169 aa),FASTA scores: opt: 179, E(): 1.8e-05, (34.6% identity in 162 aa overlap); etc. Belongs to the polypeptide deformylase family. Cofactor: binds 1 zinc ion.; Probable polypeptide deformylase Def (PDF) (formylmethionine deformylase) 518396 def 886366 def Mycobacterium tuberculosis H37Rv Probable polypeptide deformylase Def (PDF) (formylmethionine deformylase) NP_214943.1 517803 R 83332 CDS NP_214944.1 15607571 886364 518733..519041 1 NC_000962.3 Rv0430, (MTCY22G10.27), len: 102 aa. Conserved hypothetical protein, equivalent to AC30882.1|AL583923 conserved hypothetical protein from Mycobacterium leprae (102 aa). Also highly similar to CAB93047.1|SCD95A.20|AL357432 hypothetical protein from Streptomyces coelicolor (84 aa).; hypothetical protein 519041 886364 Rv0430 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214944.1 518733 D 83332 CDS NP_214945.1 15607572 886362 519073..519567 1 NC_000962.3 Rv0431, (MTCY22G10.28), len: 164 aa. Putative tuberculin related peptide; almost identical to D00815|MSGAT103_1 AT103 from Mycobacterium tuberculosis (172 aa), FASTA score: (99.4% identity in 163 aa overlap). Highly similar to to CAC30881.1|AL583923 tuberculin related peptide (AT103) from Mycobacterium leprae (167 aa). Some similarity to G550415|HRPC (282 aa), FASTA scores: opt: 120, E(): 0.36, (33.3% identity in 111 aa overlap). Potential transmembrane domain at aa 19-37.; Putative tuberculin related peptide 519567 886362 Rv0431 Mycobacterium tuberculosis H37Rv Putative tuberculin related peptide NP_214945.1 519073 D 83332 CDS NP_214946.1 15607573 886358 519600..520322 1 NC_000962.3 Rv0432, (MTCY22G10.29), len: 240 aa. sodC,periplasmic superoxide dismutase [Cu-Zn], equivalent to CAC30880.1|AL583923 superoxide dismutase precursor (Cu-Zn) from Mycobacterium leprae (240 aa); and AAK20038.1|AF326234_1 copper zinc superoxide dismutase from Mycobacterium avium subsp. paratuberculosis (226 aa). Also similar to others e.g. SODC_PHOLE|P00446 superoxide dismutase precursor (Cu-Zn) from Photobacterium leiognathi (173 aa), FASTA scores: opt: 214, E(): 5.2 e-06, (36.5% identity in 181 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Belongs to the Cu-Zn superoxide dismutase family. Possibly localized in periplasm, membrane-bound.; Periplasmic superoxide dismutase [Cu-Zn] SodC 520322 sodC 886358 sodC Mycobacterium tuberculosis H37Rv Periplasmic superoxide dismutase [Cu-Zn] SodC NP_214946.1 519600 D 83332 CDS NP_214947.1 15607574 886356 520324..521454 1 NC_000962.3 Rv0433, (MTCY22G10.30), len: 376 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. P77213|YBDK_ECOLI hypothetical 41.7 KD protein from Escherichia coli strain K12 (372 aa), FASTA scores: opt: 555, E(): 2e-30, (28.2% identity in 365 aa overlap).; hypothetical protein 521454 886356 Rv0433 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214947.1 520324 D 83332 CDS NP_214948.1 15607575 886360 521514..522167 1 NC_000962.3 Rv0434, (MTCY22G10.31), len: 217 aa. Conserved hypothetical protein, similar to AE002052_2 from Deinococcus radiodurans (213 aa), FASTA scores: opt: 258,E(): 4e-10, (31.9% identity in 213 aa overlap); SYCSLRB_122|Q55701 hypothetical 24.5 kDa protein from Synechocystis (214 aa), FASTA scores: opt: 156, E(): 0.00041, (28.4% identity in 204 aa overlap); MXABSGA_1|LON2_MYXXA|P36774 ATP-dependent protease la 2 from Myxococcus xanthus (826 aa), FASTA scores: opt: 160,E(): 0.00068, (28.4% identity in 197 aa overlap); etc.; hypothetical protein 522167 886360 Rv0434 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214948.1 521514 D 83332 CDS NP_214949.1 15607576 886352 complement(522347..524533) 1 NC_000962.3 Rv0435c, (MTCY22G10.32c), len: 728 aa. Putative conserved ATPase, similar to others e.g. SAV_SULAC|Q07590 sav protein involved in cell division from sulfolobus acidocaldarius (780 aa), FASTA scores: opt: 897, E(): 0,(34.5% identity in 693 aa overlap); NP_148637.1|7435761|B72479 transitional endoplasmic reticulum ATPase from Aeropyrum pernix (699 aa); etc. Also similar to Rv3610c and Rv2115c from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00674 AAA-protein family signature.; Putative conserved ATPase 524533 886352 Rv0435c Mycobacterium tuberculosis H37Rv Putative conserved ATPase NP_214949.1 522347 R 83332 CDS NP_214950.1 15607577 886385 complement(524530..525390) 1 NC_000962.3 Rv0436c, (MTCY22G10.33c), len: 286 aa. Probable pssA, PS synthase (CDP-diacylglycerol--serine O-phosphatidyltransferase) (see citation below), integral membrane protein, equivalent to AL035159|MLCB1450_9|T44730 from Mycobacterium leprae (300 aa), FASTA scores: opt: 1506, E(): 0, (77.9% identity in 285 aa overlap). Also highly similar to others e.g. NP_108059.1|14027250|BAB54204.1|AP003012 phosphatidylserine synthase from Mesorhizobium loti (248 aa); PSS_BACSU|P39823 cdp-diacylglycerol--serine o-phosphatidyltransferase from Bacillus subtilis (177 aa), FASTA scores: opt: 277, E(): 9.9e-12, (33.3% identity in 183 aa overlap); etc. Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family.; Probable CDP-diacylglycerol--serine O-phosphatidyltransferase PssA (PS synthase) (phosphatidylserine synthase) 525390 pssA 886385 pssA Mycobacterium tuberculosis H37Rv Probable CDP-diacylglycerol--serine O-phosphatidyltransferase PssA (PS synthase) (phosphatidylserine synthase) NP_214950.1 524530 R 83332 CDS NP_214951.1 15607578 886350 complement(525387..526082) 1 NC_000962.3 Rv0437c, (MTV037.01c), len: 231 aa (start uncertain). Possible psd, phosphatidylserine decarboxylase , equivalent to CAC29819.1|AL583918 conserved hypothetical protein from Mycobacterium leprae (243 aa); and highly similar to MLCB1450.11|T44729|4154044|CAA22695.1|AL035159 hypothetical protein from Mycobacterium leprae (202 aa),FASTA score: (74.6% identity in 197 aa overlap). Also similar to other phosphatidylserine decarboxylases e.g. NP_108058.1|14027249|BAB54203.1|AP003012 phosphatidylserine decarboxylase from Mesorhizobium loti (232 aa); AAK86872|g15156090|AGR_C_1963 phosphatidylserine decarboxylase from Agrobacterium tumefaciens (244 aa); AAG12422.1|AY005137|Psd phosphatidylserine decarboxylase from Chlorobium tepidum (216 aa); etc.; Possible phosphatidylserine decarboxylase Psd (PS decarboxylase) 526082 psd 886350 psd Mycobacterium tuberculosis H37Rv Possible phosphatidylserine decarboxylase Psd (PS decarboxylase) NP_214951.1 525387 R 83332 CDS YP_177725.1 57116730 886348 complement(526143..527360) 1 NC_000962.3 Rv0438c, (MTV037.02c), len: 405 aa. Probable moeA2,molybdenum cofactor biosynthesis protein, highly similar to many e.g. Y10817|ANY10817_2 from A. nicotinovorans (429 aa), FASTA scores: opt: 786, E(): 0, (39.2% identity in 398 aa overlap); etc. Also similar to MOEA1|Rv0994|MTCI237.08|O05577 probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (426 aa), FASTA scores: opt: 667, E(): 2e-32, (36.5% identity in 425 aa overlap). Note that previously known as moeA3.; Probable molybdopterin biosynthesis protein MoeA2 527360 moeA2 886348 moeA2 Mycobacterium tuberculosis H37Rv Probable molybdopterin biosynthesis protein MoeA2 YP_177725.1 526143 R 83332 CDS NP_214953.1 15607580 886342 complement(527379..528314) 1 NC_000962.3 Rv0439c, (MTV037.03c), len: 311 aa. Probable dehydrogenase/reductase, equivalent to AL035159|MLCB1450_6|T44727 probable oxidoreductase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1360,E(): 0, (69.2% identity in 302 aa overlap). Also highly similar to various oxidoreductases, generally dehydrogenases/reductases e.g. PA5031|C83017|9951320|AAG08416.1|AE004916_5|AE004916 probable short chain dehydrogenase from Pseudomonas aeruginosa (309 aa); Q03326|OXIR_STRAT probable oxidoreductase from Streptomyces antibioticus (298 aa),FASTA scores: opt: 400, E(): 1.2e-18, (34.6% identity in 298 aa overlap); etc.; Probable dehydrogenase/reductase 528314 886342 Rv0439c Mycobacterium tuberculosis H37Rv Probable dehydrogenase/reductase NP_214953.1 527379 R 83332 CDS NP_214954.1 15607581 886354 528608..530230 1 NC_000962.3 Rv0440, (MTV037.04), len: 540 aa. GroEL2 (alternate gene names: groL2, groEL-2, hsp65, hsp60), 60 kDa chaperonin 2 (see Shinnick 1987). Purified 65 kDa antigen can elicit a strong delayed-type hypersensitivity reaction in experimental animals infected with M. tuberculosis. This protein is one of the major immunoreactive proteins of the mycobacteria. This antigen contains epitopes that are common to various species of mycobacteria. Contains PS00296 Chaperonins cpn60 signature. Belongs to the chaperonin (HSP60) family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010).; 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A) 530230 groEL2 886354 groEL2 Mycobacterium tuberculosis H37Rv 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A) NP_214954.1 528608 D 83332 CDS NP_214955.1 15607582 886344 complement(530296..530724) 1 NC_000962.3 Rv0441c, (MTV037.05c), len: 142 aa. Hypothetical unknown protein.; Hypothetical protein 530724 886344 Rv0441c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214955.1 530296 R 83332 CDS YP_177726.2 448824744 886340 complement(530751..532214) 1 NC_000962.3 Rv0442c, (MTV037.06c), len: 487 aa. PPE10, Member of the Mycobacterium tuberculosis PPE family, nearly identical to hypothetical protein from Mycobacterium tuberculosis (strain Erdman) and to AN5S46909_1 protein fragment from Mycobacterium bovis (302 aa); P42611|YHS6_MYCTU hypothetical 50.6 kDa protein (517 aa), FASTA scores: opt: 3144, E(): 0, (98.4 identity in 492 aa overlap); and S46909|S46909_1 (302 aa), FASTA scores: opt: 1897, E(): 0,(98.0% identity in 302 aa overlap). Nucleotide position 532097 in the genome sequence has been corrected, T:C resulting in K40E.; PPE family protein PPE10 532214 PPE10 886340 PPE10 Mycobacterium tuberculosis H37Rv PPE family protein PPE10 YP_177726.2 530751 R 83332 CDS NP_214957.1 15607584 886336 532396..532911 1 NC_000962.3 Rv0443, (MTV037.07), len: 171 aa. Conserved protein,highly similar to AL049863|SC5H1_23|T35339 hypothetical protein from Streptomyces coelicolor (171 aa), FASTA scores: opt: 561, E(): 2.3e-32, (49.7% identity in 165 aa overlap); and CAC42482.1|AJ318385 hypothetical protein from Amycolatopsis mediterranei (163 aa).; hypothetical protein 532911 886336 Rv0443 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214957.1 532396 D 83332 CDS NP_214958.1 15607585 886346 complement(533091..533789) 1 NC_000962.3 Rv0444c, (MTV037.08c), len: 232 aa. RskA, regulator of SigK (See Said-Salim et al., 2006); C-terminus similar to P12752|Y24K_STRGR hypothetical 24.7 kDa protein from Streptomyces griseus (238 aa), FASTA scores: opt: 207, E(): 2.2e-05, (32.9% identity in 158 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al.,2010).; Anti-sigma factor RskA (regulator of sigma K) 533789 rskA 886346 rskA Mycobacterium tuberculosis H37Rv Anti-sigma factor RskA (regulator of sigma K) NP_214958.1 533091 R 83332 CDS NP_214959.1 15607586 886334 complement(533833..534396) 1 NC_000962.3 Rv0445c, (MTV037.09c), len: 187 aa. sigK,alternative RNA polymerase sigma factor (see citations below), highly similar to others e.g. 5531433|CAB50938.1|AL096849|T36745 probable RNA polymerase sigma factor from Streptomyces coelicolor (185 aa); NP_105607.1|14024791|BAB51393.1|AP003005 RNA polymerase sigma factor from Mesorhizobium loti (179 aa); 1654108|AAB17906.1|U11283|A58883 probable transcription initiation factor sigma E from Rhodobacter phaeroides (168 aa), FASTA scores: opt: 299, E(): 2e-14, (32.7% identity in 168 aa overlap); Q45585|SIGW_BACSU RNA polymerase sigma factor SIGW from Bacillus subtilis (187 aa), FASTA scores: opt: 213, E(): 2.9e-08, (26.8% identity in 179 aa overlap); etc.; Alternative RNA polymerase sigma factor SigK 534396 sigK 886334 sigK Mycobacterium tuberculosis H37Rv Alternative RNA polymerase sigma factor SigK NP_214959.1 533833 R 83332 CDS NP_214960.1 15607587 886332 complement(534445..535215) 1 NC_000962.3 Rv0446c, (MTV037.10c), len: 256 aa. Possible conserved transmembrane protein, similar at N-terminus to U1740AF|U15183|MLU15183_40 from Mycobacterium leprae (117 aa), FASTA scores: opt: 175, E(): 2.5e-05, (62.5% identity in 40 aa overlap); and at C-terminus to AL021529|SC10A5_3 from Streptomyces coelicolor (226 aa), FASTA scores: opt: 207, E(): 9.8e-07, (34.2% identity in 114 aa overlap). Also similar to others hypothetical proteins e.g. AAK04680.1|AE006291_14|AE006291 hypothetical protein from Lactococcus lactis subsp. lactis (257 aa).; Possible conserved transmembrane protein 535215 886332 Rv0446c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_214960.1 534445 R 83332 CDS NP_214961.1 15607588 886330 complement(535224..536507) 1 NC_000962.3 Rv0447c, (MTV037.11c), len: 427 aa (start uncertain). Probable ufaA1,cyclopropane-fatty-acyl-phospholipid synthase, similar to others e.g. NP_102178.1|14021351|BAB47964.1|AP002994 cyclopropane-fatty-acyl-phospholipid synthase from Mesorhizobium loti (378 aa); B82240|9655593|AAF94281.1|AE004192 cyclopropane-fatty-acyl-phospholipid synthase from Vibrio cholerae (432 aa); P30010|CFA_ECOLI cyclopropane-fatty-acyl-phospholipid synthase from Escherichia coli strain K-12 (382 aa); X55704|PPLPD_3 LPD-3 from P.putida (394 aa), FASTA scores: opt: 556, E(): 2.8e-30, (33.3% identity in 387 aa overlap); AE0005|HPAE000557_9 from Helicobacter pylori (389 aa),FASTA scores: opt: 539, E(): 3.9e-29, (34.3% identity in 382 aa overlap).; Probable cyclopropane-fatty-acyl-phospholipid synthase UfaA1 (cyclopropane fatty acid synthase) (CFA synthase) 536507 ufaA1 886330 ufaA1 Mycobacterium tuberculosis H37Rv Probable cyclopropane-fatty-acyl-phospholipid synthase UfaA1 (cyclopropane fatty acid synthase) (CFA synthase) NP_214961.1 535224 R 83332 CDS NP_214962.1 15607589 886328 complement(536504..537169) 1 NC_000962.3 Rv0448c, (MTV037.12c), len: 221 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. Z74841|BOD5A2_1 from B. oleracea (283 aa),FASTA scores: opt: 257, E(): 1.4e-10, (32.0% identity in 197 aa overlap); etc. Some similarity to U15183|MLU15183_38 from Mycobacterium leprae (82 aa), FASTA scores: opt: 134,E(): 0.014, (71.0% identity in 31 aa overlap).; hypothetical protein 537169 886328 Rv0448c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214962.1 536504 R 83332 CDS NP_214963.1 15607590 886326 complement(537229..538548) 1 NC_000962.3 Rv0449c, (MTV037.13c), len: 439 aa. Conserved hypothetical protein, some similarity with several hypothetical proteins and various enzymes e.g. AAK24569.1|AE005927 amine oxidase, flavin-containing from Caulobacter crescentus (454 aa); BAB02771.1|AB023036 mycolic acid methyl transferase-like protein from Arabidopsis thaliana (842 aa); BAB01742.1|AP000374 protein which contains similarity to cyclopropane fatty acid synthase from Arabidopsis thaliana (793 aa); etc. Has hydrophobic stretch at N-terminus.; hypothetical protein 538548 886326 Rv0449c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214963.1 537229 R 83332 CDS NP_214964.1 15607591 886323 complement(538588..541491) 1 NC_000962.3 Rv0450c, (MTV037.14c), len: 967 aa. Probable mmpL4,conserved transmembrane transport protein (see citations below), member of RND superfamily, equivalent to U1740V|P54881|YV34_MYCLE hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 5051, E(): 0, (78.4% identity in 962 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. Z83860|MTCY98.08 (962 aa), FASTA scores: opt: 3917, E(): 0, (61.3% identity in 950 aa overlap),MTCY20G9.34, etc. Contains PS00211 ABC transporters family signature. Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL4 541491 mmpL4 886323 mmpL4 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL4 NP_214964.1 538588 R 83332 CDS NP_214965.1 15607592 886321 complement(541488..541910) 1 NC_000962.3 Rv0451c, (MTV037.15c), len: 140 aa. Probable mmpS4,conserved membrane protein (see citations below),equivalent to U1740W|P54880|YV33_MYCLE hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 727, E(): 0, (75.9% identity in 137 aa overlap). Also similar to other Mycobacterial proteins e.g. Z84725|MTCY04D9.16c from Mycobacterium tuberculosis (142 aa), FASTA scores: opt: 451, E(): 3.2e-24, (50.0% identity in 138 aa overlap); etc. Belongs to the MmpS family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein MmpS4 541910 mmpS4 886321 mmpS4 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein MmpS4 NP_214965.1 541488 R 83332 CDS NP_214966.1 15607593 886319 542142..542852 1 NC_000962.3 Rv0452, (MTV037.16), len: 236 aa. Possible transcriptional regulator, similar to several putative TetR-family transcriptional regulators from Streptomyces coelicolor. Also similar in N-terminus to U1740Y|U15183|MLU15183_33 from Mycobacterium leprae (67 aa), FASTA score: (76.1% identity in 67 aa overlap). Contains probable helix-turn-helix motif at aa 44-65 (Score 1727, +5.07 SD).; Possible transcriptional regulatory protein 542852 886319 Rv0452 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214966.1 542142 D 83332 CDS YP_177727.1 57116732 886317 543174..544730 1 NC_000962.3 Rv0453, (MTV037.17), len: 518 aa. PPE11, Member of the Mycobacterium tuberculosis PPE family, similar to many e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 882, E(): 7e-31, (41.8% identity in 514 aa overlap).; PPE family protein PPE11 544730 PPE11 886317 PPE11 Mycobacterium tuberculosis H37Rv PPE family protein PPE11 YP_177727.1 543174 D 83332 CDS NP_214968.1 15607595 886339 544835..545185 1 NC_000962.3 Rv0454, (MTV037.18), len: 116 aa (start uncertain). Conserved hypothetical protein, showing similarity with AAA63007.1|U15183 hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 151, E(): 0.0019,(31.5% identity in 89 aa overlap).; hypothetical protein 545185 886339 Rv0454 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214968.1 544835 D 83332 CDS NP_214969.1 15607596 886314 complement(545375..545821) 1 NC_000962.3 Rv0455c, (MTV037.19c), len: 148 aa. Conserved protein, equivalent to CAC31896.1|AL583925 possible secreted protein from Mycobacterium leprae (153 aa). Has hydrophobic stretch at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 545821 886314 Rv0455c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214969.1 545375 R 83332 CDS NP_214970.1 15607597 886312 complement(545889..546803) 1 NC_000962.3 Rv0456c, (MTCI429A.02, MTV037.20c), len: 304 aa. Probable echA2, enoyl-CoA hydratase, similar to other enoyl-CoA hydratases e.g. Q13011 peroxisomal enoyl-CoA hydratase-like protein (328 aa), FASTA scores: opt: 209,E(): 5.3e-07, (31.7% identity in 142 aa overlap). Also similar to several other proteins from Mycobacterium tuberculosis e.g. MTCY09F9.29 FASTA score: (32.9% identity in 146 aa overlap); and MTI376.01c.; enoyl-CoA hydratase EchA2 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 546803 echA2 886312 echA2 Mycobacterium tuberculosis H37Rv enoyl-CoA hydratase EchA2 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_214970.1 545889 R 83332 CDS YP_177621.1 57116733 3205039 complement(547076..547357) 1 NC_000962.3 Rv0456A, len: 93 aa. Possible mazF1, toxin, part of toxin-antitoxin (TA) operon with Rv0456B (See Pandey and Gerdes, 2005; Zhu et al., 2006); N-terminus highly similar to N-terminal part of P71650|Rv2801c|MT2869|MTCY16B7.42 conserved hypothetical protein from Mycobacterium tuberculosis (118 aa), FASTA scores: opt: 303, E(): 1e-14,(60.44% identity in 91 aa overlap). Also some similarity in part with other hypothetical proteins e.g. Q9PHH8|XFA0027 Plasmid maintenance protein from Xylella fastidiosa (108 aa), FASTA scores: opt: 169, E(): 3.9e-05, (50.820% identity in 61 aa overlap).; Possible toxin MazF1 547357 mazF1 3205039 mazF1 Mycobacterium tuberculosis H37Rv Possible toxin MazF1 YP_177621.1 547076 R 83332 CDS YP_007409102.1 448815229 14515889 complement(547344..547517) 1 NC_000962.3 Rv0456B, len: 57 aa. Possible mazE1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0456A (See Pandey and Gerdes, 2005; Zhu et al., 2006).; Possible antitoxin MazE1 547517 mazE1 14515889 mazE1 Mycobacterium tuberculosis H37Rv Possible antitoxin MazE1 YP_007409102.1 547344 R 83332 CDS NP_214971.1 15607598 886310 complement(547586..549607) 1 NC_000962.3 Rv0457c, (MTCI429A.01, MTV038.01c), len: 673 aa. Probable peptidase, similar to many e.g. NP_102851.1|14022026|BAB48637.1 probable endopeptidase from Mesorhizobium loti (687 aa); Y4NA_RHISN|P55577 probable peptidase (726 aa), FASTA scores: opt: 1126, E(): 0, (40.9% identity in 491 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY369.26 FASTA score: (33.8% identity in 299 aa overlap).; Probable peptidase 549607 886310 Rv0457c Mycobacterium tuberculosis H37Rv Probable peptidase NP_214971.1 547586 R 83332 CDS NP_214972.1 15607599 886306 549675..551198 1 NC_000962.3 Rv0458, (MTV038.02), len: 507 aa. Probable aldehyde dehydrogenase, highly similar to many, closest to P46369|THCA_RHOER EPTC-inducible aldehyde dehydrogenase from Rhodococcus erythropolis (506 aa), FASTA scores: opt: 2767, E(): 0, (79.7% identity in 507 aa overlap); AAC13641.1|AF029733 chloroacetaldehyde dehydrogenase from Xanthobacter autotrophicus (505 aa), FASTA scores: opt: 2563, E(): 0, (75.4% identity in 492 aa overlap); Q9RJZ6|DHAL_STRCO probable aldehyde dehydrogenase from Streptomyces coelicolor (507 aa). Also similar to other semialdehyde dehydrogenases in Mycobacterium tuberculosis e.g. Rv0768, Rv2858c. Belongs to the aldehyde dehydrogenases family.; Probable aldehyde dehydrogenase 551198 886306 Rv0458 Mycobacterium tuberculosis H37Rv Probable aldehyde dehydrogenase NP_214972.1 549675 D 83332 CDS NP_214973.1 15607600 886372 551198..551689 1 NC_000962.3 Rv0459, (MTV038.03), len: 163 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins. Note that highly similar to products of unidentified ORFs in Xanthobacter autotrophicus, AF029733_2 (139 aa), and Rhodococcus erythropolis, REREUTP BC_1 (186 aa). Like MTV038.03, these ORF's are linked to aldehyde dehydrogenase genes. FASTA scores: AF0297|AF029733_2 (139 aa), opt: 439, E(): 6.2e-24, (50.0% identity in 126 aa overlap); and L24492|REREUTPBC_1 (186 aa), opt: 347, E(): 2.1e-17, (52.7% identity in 169 aa overlap). N-terminus also highly similar to AAA63041.1|U15183 ethanolamine permease (eutP) match from Mycobacterium leprae (53 aa).; hypothetical protein 551689 886372 Rv0459 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214973.1 551198 D 83332 CDS NP_214974.1 15607601 886304 551749..551988 1 NC_000962.3 Rv0460, (MTV038.04), len: 79 aa. Conserved hydrophobic protein, highly similar AAA63024.1|U15183 hypothetical protein from Mycobacterium leprae (56 aa),FASTA scores: opt: 197, E(): 3.7e-09, (63.8% identity in 47 aa overlap).; Conserved hydrophobic protein 551988 886304 Rv0460 Mycobacterium tuberculosis H37Rv Conserved hydrophobic protein NP_214974.1 551749 D 83332 CDS NP_214975.1 15607602 886302 552026..552550 1 NC_000962.3 Rv0461, (MTV038.05), len: 174 aa (start uncertain). Probable transmembrane protein. Nucleotide position 552085 in the genome sequence has been corrected, A:G resulting in Q20Q.; Probable transmembrane protein 552550 886302 Rv0461 Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_214975.1 552026 D 83332 CDS NP_214976.1 15607603 886300 552614..554008 1 NC_000962.3 Rv0462, (MTV038.06), len: 464 aa. LpdC (alternate gene name: TB49.2, CIP50), dihydrolipoamide dehydrogenase (see Argyrou & Blanchard 2001), equivalent to AAA63016.1|U15183 lipoamide dehydrogenase from Mycobacterium leprae (467 aa), FASTA scores: opt: 2583,E(): 0, (83.1% identity in 467 aa overlap). Also similar to to many e.g. P50970|DLDH_ZYMMO|X82291|ZMLPD_1 dihydrolipoamide dehydrogenase from Z.mobilis (466 aa),FASTA scores: opt: 1198, E(): 0, (42.4 % identity in 465 aa overlap); etc. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Binds to coronin-1 in BCG and M. tuberculosis - coronin-1 is retained on phagosomes and phagosome maturation is arrested (See Deghmane et al.,2007). LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011).; Dihydrolipoamide dehydrogenase LpdC (lipoamide reductase (NADH)) (lipoyl dehydrogenase) (dihydrolipoyl dehydrogenase) (diaphorase) 554008 lpdC 886300 lpdC Mycobacterium tuberculosis H37Rv Dihydrolipoamide dehydrogenase LpdC (lipoamide reductase (NADH)) (lipoyl dehydrogenase) (dihydrolipoyl dehydrogenase) (diaphorase) NP_214976.1 552614 D 83332 CDS NP_214977.1 15607604 886299 554016..554309 1 NC_000962.3 Rv0463, (MTV038.07), len: 97 aa. Probable conserved transmembrane protein, highly similar to AAA63017.1|U15183 hypothetical protein from Mycobacterium leprae (101 aa),FASTA scores: opt: 364, E(): 4e-21, (57.9% identity in 95 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved membrane protein 554309 886299 Rv0463 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214977.1 554016 D 83332 CDS NP_214978.1 15607605 886296 complement(554313..554885) 1 NC_000962.3 Rv0464c, (MTV038.08c), len: 190 aa. Conserved protein, highly similar to CAC31982.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (188 aa). Also some similarity with Rv1531|AL022000|MTV045_5|D70820 hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 9.6e-10, (30.9% identity in 175 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 554885 886296 Rv0464c Mycobacterium tuberculosis H37Rv hypothetical protein NP_214978.1 554313 R 83332 CDS NP_214979.1 15607606 886320 complement(554882..556306) 1 NC_000962.3 Rv0465c, (MTV038.09c), len: 474 aa. Probable transcriptional regulator, highly similar to AC44331.1|AL596102 putative DNA-binding protein from Streptomyces coelicolor (489 aa); and similar to several hypothetical proteins and others transcriptional regulators. Some similarity in N-terminal region (1-100 aa) with repressors e.g. P06153|RPC_BPPH1 immunity repressor protein (144 aa), FASTA scores: opt: 130, E(): 0.084,(27.0% identity in 100 aa overlap). Very similar to Rv1129c|Z95585|MTCY22G8.18c from Mycobacterium tuberculosis (486 aa), FASTA scores: opt: 1475, E(): 0, (47.4% identity in 468 aa overlap). Contains probable helix-turn-helix motif at aa 19-40 (1827, +5.41 SD).; Probable transcriptional regulatory protein 556306 886320 Rv0465c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_214979.1 554882 R 83332 CDS NP_214980.1 15607607 886294 556458..557252 1 NC_000962.3 Rv0466, (MTV038.10), len: 264 aa. Conserved protein,equivalent to CAC31980.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (264 aa). Similar to Rv2001|Z74025|MTCY39.17c hypothetical 28.7 KDA protein from Mycobacterium tuberculosis (250 aa), FASTA scores: opt: 592, E(): 0, (38.0% identity in 263 aa overlap). Some similarity to several thioesterases e.g. Q42561|ATACPTE17_1 acyl-(acyl carrier protein) thioester from A. thaliana (362 aa), FASTA scores: E(): 0.0092, (24.4% identity in 197 aa overlap).; hypothetical protein 557252 886294 Rv0466 Mycobacterium tuberculosis H37Rv hypothetical protein NP_214980.1 556458 D 83332 CDS YP_177728.1 57116734 886291 557527..558813 1 NC_000962.3 Rv0467, (MTV038.11), len: 428 aa. Icl1, isocitrate lyase (see citations below), highly similar to many,closest to Z29367|RFISCILY_1 from R. fascians (429 aa),FASTA scores: opt: 2359, E(): 0, (80.7% identity in 429 aa overlap). Belongs to the isocitrate lyase family. Has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006; Gould et al., 2006). Predicted possible vaccine candidate (See Zvi et al.,2008).; Isocitrate lyase Icl (isocitrase) (isocitratase) 558813 icl1 886291 icl1 Mycobacterium tuberculosis H37Rv Isocitrate lyase Icl (isocitrase) (isocitratase) YP_177728.1 557527 D 83332 CDS NP_214982.1 15607609 886288 558895..559755 1 NC_000962.3 Rv0468, (MTV038.12), len: 286 aa. fadB2,3-hydroxybutyryl-CoA dehydrogenase, equivalent to CAC31978.1|AL583925 3-hydroxyacyl-CoA dehydrogenase from Mycobacterium leprae (287 aa). Also similar to many 3-hydroxybutyryl-CoA dehydrogenases e.g. U32229|BJU32229_1 beta-hydroxybutyryl coenzyme A dehydrogenase from Bradyrhizobium japonicum (293 aa), FASTA scores: opt: 771,E(): 0, (45.7% identity in 282 aa overlap). Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.; 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) 559755 fadB2 886288 fadB2 Mycobacterium tuberculosis H37Rv 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) NP_214982.1 558895 D 83332 CDS YP_177729.1 57116735 886286 559888..560748 1 NC_000962.3 Rv0469, (MTV038.13), len: 286 aa. Possible umaA,mycolic acid synthase (see citations below), highly similar to CAC30854.1|AL583923 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa),FASTA scores: opt: 1402, E(): 0, (69.6% identity in 286 aa overlap). Also very similar to mycobacterial methyltransferases e.g. U77466|CmaD|MBU77466_1 (286 aa); MTCY20H10.26c|Z92772|MTY20H10_27 (296 aa); highly similar to CFA1_MYCTU|Q11195|U66108|MTU66108_1 cyclopropane-fatty-acyl-phospholipid synthase 1 (287 aa),FASTA scores: opt: 1360, E(): 0, (67.8% identity in 286 aa overlap) (see citation below); and very similar also to methoxy mycolic acid synthase 1 from Mycobacterium tuberculosis e.g. MTU66108_1 (286 aa). Note that previously known as umaA1.; Possible mycolic acid synthase UmaA 560748 umaA 886286 umaA Mycobacterium tuberculosis H37Rv Possible mycolic acid synthase UmaA YP_177729.1 559888 D 83332 CDS YP_177730.1 57116736 886284 complement(560848..561711) 1 NC_000962.3 Rv0470c, (MTV038.14), len: 287 aa. PcaA (previously known as umaA2), mycolic acid synthase (cyclopropane synthase) (see citations below), equivalent to CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa); and highly similar to S72886|B2168_F3_130|467038|AAA17222.1|U00018 hypothetical protein from Mycobacterium leprae (308 aa); Q11195|CFA1_MYCTU cyclopropane-fatty-acyl-phospholipid synthase 1 (cyclopropane mycolic acid synthase 1) (287 aa) (see Glickman et al., 2000); U27357|MTU27357_1 cyclopropane mycolic acid synthase from Mycobacterium tuberculosis (287 aa), FASTA scores: opt: 1415, E(): 0, (72.8% identity in 287 aa overlap); and related enzymes e.g. MTCY20H10.25c|Z92772|MTY20H10_26 (287 aa), FASTA scores: opt: 1387, E(): 0, (72.5% identity in 287 aa overlap).; Mycolic acid synthase PcaA (cyclopropane synthase) 561711 pcaA 886284 pcaA Mycobacterium tuberculosis H37Rv Mycolic acid synthase PcaA (cyclopropane synthase) YP_177730.1 560848 R 83332 CDS YP_177622.1 57116737 3205059 complement(561854..562294) 1 NC_000962.3 Rv0470A, len: 146 aa. Hypothetical unknown protein. GC plot suggests CDS for Cys-rich protein, could possibly be continuation of Rv0471c but no frameshift found to allow this. Sequence same in Mycobacterium bovis and Mycobacterium tuberculosis strain CDC1551. Weak hits to Cys-rich region (aa 258-314) of D63395|D63395_1 mRNA for NOTCH4 from Homo sapiens (1095 aa), FASTA scores: opt: 132,E(): 1.1, (39.35% identity in 61 aa overlap).; Hypothetical protein 562294 3205059 Rv0470A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177622.1 561854 R 83332 CDS NP_214985.1 15607612 886280 complement(562225..562713) 1 NC_000962.3 Rv0471c, (MTV038.15c), len: 162 aa. Hypothetical unknown protein.; Hypothetical protein 562713 886280 Rv0471c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214985.1 562225 R 83332 CDS NP_214986.1 15607613 886308 complement(562723..563427) 1 NC_000962.3 Rv0472c, (MTV038.16c), len: 234 aa. Probable regulatory protein, possibly TetR family, equivalent to CAC31974.1|AL583925 possible TetR-family transcriptional regulator from Mycobacterium leprae (233 aa). Also similar to CAC01492.1|AL391017 putative transcriptional regulatory protein from Streptomyces coelicolor (218 aa); and CAC01371.1|AL390975 putative TetR-family transcriptional regulator from Streptomyces coelicolor (228 aa). Also similar to AL0212|MTV012_65 from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 327, E(): 1.8e-15, (31.0% identity in 232 aa overlap); and Z95120|MTCY07D11.18c (228 aa), FASTA scores: opt: 190, E(): 4.4e-06, (23.1% identity in 186 aa overlap). Contains probable helix-turn-helix doimain at aa 45-66 (Score 1429, +4.05 SD).; Probable transcriptional regulatory protein (possibly TetR-family) 563427 886308 Rv0472c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (possibly TetR-family) NP_214986.1 562723 R 83332 CDS NP_214987.2 448824745 886279 563564..564934 1 NC_000962.3 Rv0473, (MTV038.17), len: 456 aa. Possible conserved transmembrane protein, showing some similarity to hypothetical proteins e.g. NP_102800.1|14021975|BAB48586.1|AP002996 hypothetical protein from Mesorhizobium loti (431 aa); P39385|YJIN_ECOLI|YJIN|B4336 hypothetical 48.2 kDa protein (potential integral membrane protein) from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 396, E(): 9.8e-19,(31.8 % identity in 424 aa overlap); etc. Nucleotide position 563577 in the genome sequence has been corrected,A:G resulting in K5R.; Possible conserved transmembrane protein 564934 886279 Rv0473 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_214987.2 563564 D 83332 CDS NP_214988.1 15607615 886276 565021..565443 1 NC_000962.3 Rv0474, (MTV038.18), len: 140 aa. Probable transcriptional regulator, highly similar to others e.g. CAC04034.1|AL391406 putative DNA-binding protein from Streptomyces coelicolor (141 aa); N-terminus of NP_104173.1|14023352|BAB49959.1|AP003000 transcriptional regulator from Mesorhizobium loti (219 aa); N-terminus of A83618|PA0225 probable transcription regulator from Pseudomonas aeruginosa (179 aa); SINR_BACSU|P06533 sinr protein from Bacillus subtilis (111 aa), FASTA scores: opt: 147, E(): 8.9e-06, (30.6% identity in 111 aa overlap). Also similar to other hypothetical proteins e.g. X66407|RRPHAS_1|ORF1 from Rhodococcus ruber (171 aa), FASTA scores: opt: 280, E(): 4.8e-12, (43.6% identity in 117 aa overlap). Also similar to Rv2745c from Mycobacterium tuberculosis. Contains probable helix-turn-helix domain at aa 35-56 (Score 1709, +5.01 SD).; Probable transcriptional regulatory protein 565443 886276 Rv0474 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_214988.1 565021 D 83332 CDS NP_214989.1 15607616 886272 565797..566396 1 NC_000962.3 Rv0475, hbhA (MTCY20G9.01), len: 199 aa. HbhA,iron-regulated heparin-binding hemagglutinin (see citations below), equivalent to CAC31971.1|AL583925 possible hemagglutinin from Mycobacterium leprae (188 aa). Contains possible N-terminal signal sequence and K-a-rich region at C-terminus: subcellular location: surface associated. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Iron-regulated heparin binding hemagglutinin HbhA (adhesin) 566396 hbhA 886272 hbhA Mycobacterium tuberculosis H37Rv Iron-regulated heparin binding hemagglutinin HbhA (adhesin) NP_214989.1 565797 D 83332 CDS NP_214990.1 15607617 886282 566508..566771 1 NC_000962.3 Rv0476, (MTCY20G9.02), len: 87 aa. Possible conserved transmembrane protein, equivalent to CAC31970.1|AL583925 conserved membrane protein from Mycobacterium leprae (95 aa). Also highly similar to CAC04036.1|AL391406 putative membrane protein from Streptomyces coelicolor (113 aa). Contains PS00606 Beta-ketoacyl synthases active site.; Possible conserved transmembrane protein 566771 886282 Rv0476 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_214990.1 566508 D 83332 CDS NP_214991.1 15607618 886273 566776..567222 1 NC_000962.3 Rv0477, (MTCY20G9.03), len: 148 aa. Possible conserved secreted protein, equivalent to CAC31969.1|AL583925 hypothetical protein from Mycobacterium leprae (123 aa). Also similar to G83406|PA1914 conserved hypothetical protein from Pseudomonas aeruginosa (408 aa). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved secreted protein 567222 886273 Rv0477 Mycobacterium tuberculosis H37Rv Possible conserved secreted protein NP_214991.1 566776 D 83332 CDS NP_214992.1 15607619 888425 567222..567896 1 NC_000962.3 Rv0478, (MTCY20G9.04), len: 224 aa. Probable deoC,deoxyribose-phosphate aldolase, equivalent to Q9CB45|DEOC_MYCLE deoxyribose-phosphate aldolase from Mycobacterium leprae (226 aa). Also highly similar to others e.g. DEOC_BACSU|P39121 from Bacillus subtilis (214 aa), FASTA scores: opt: 543, E(): 1.4e-26, (45.9% identity in 209 aa overlap); etc. Belongs to the DEOC/FBAB family of aldolases, DEOC subfamily.; Probable deoxyribose-phosphate aldolase DeoC (phosphodeoxyriboaldolase) (deoxyriboaldolase) 567896 deoC 888425 deoC Mycobacterium tuberculosis H37Rv Probable deoxyribose-phosphate aldolase DeoC (phosphodeoxyriboaldolase) (deoxyriboaldolase) NP_214992.1 567222 D 83332 CDS NP_214993.1 15607620 885535 complement(567921..568967) 1 NC_000962.3 Rv0479c, (MTCY20G9.04c), len: 348 aa. Probable conserved membrane protein, equivalent to CAC31967.1|AL583925 possible secreted protein from Mycobacterium leprae (254 aa); and C-terminus highly similar to AAF74996.1|AF143402_1|AF143402 putative multicopper oxidase from Mycobacterium avium (149 aa). Contains hydrophobic domain in centre of protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved membrane protein 568967 885535 Rv0479c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_214993.1 567921 R 83332 CDS NP_214994.2 57116738 887163 complement(568964..569806) 1 NC_000962.3 Rv0480c, (MTCY20G9.06c), len: 280 aa. Possible amidohydrolase, highly similar to NP_302587.1|NC_002677|CAC31966.1|AL583925 putative hydrolase from Mycobacterium leprae (271 aa). Also similar to other hydrolases and hypothetical proteins e.g. NP_601985.1|NC_003450 Predicted amidohydrolase from Corynebacterium glutamicum (266 aa); NP_459623.1|NC_003197 putative hydrolase from Salmonella typhimurium LT2 (262 aa); AL096822|SCGD3_8|NP_627996.1|NC_003888 probable hydrolase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 368, E(): 6.1e-15, (34.2% identity in 272 aa overlap); YAUB_SCHPO|Q10166 hypothetical 35.7 kDa protein c26a3.11 from S. pombe (322 aa), FASTA scores: opt: 338,E():1.4e-13, (30.3% identity in 277 aa overlap); etc. Start changed since first submission (-60 aa).; Possible amidohydrolase 569806 887163 Rv0480c Mycobacterium tuberculosis H37Rv Possible amidohydrolase NP_214994.2 568964 R 83332 CDS NP_214995.1 15607622 887161 complement(569988..570512) 1 NC_000962.3 Rv0481c, (MTCY20G9.07c), len: 174 aa. Hypothetical unknown protein.; Hypothetical protein 570512 887161 Rv0481c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_214995.1 569988 R 83332 CDS NP_214996.1 15607623 887169 570539..571648 1 NC_000962.3 Rv0482, (MTCY20G9.08), len: 369 aa. Probable murB,UDP-N-acetylenolpyruvoylglucosamine reductase (see citation below), equivalent to CAC31964.1|AL583925 UDP-N-acetylenolpyruvoylglucosamine reductase from Mycobacterium leprae (367 aa). Also highly similar to others e.g. MURB_ECOLI|P08373 UDP-N-acetylenolpyruvoylglucosamine reductase from Escherichia coli (342 aa), FASTA scores: opt: 292, E(): 6.3e-12, (33.5% identity in 355 aa overlap); etc. Belongs to the MurB family. Cofactor: FAD.; Probable UDP-N-acetylenolpyruvoylglucosamine reductase MurB (UDP-N-acetylmuramate dehydrogenase) 571648 murB 887169 murB Mycobacterium tuberculosis H37Rv Probable UDP-N-acetylenolpyruvoylglucosamine reductase MurB (UDP-N-acetylmuramate dehydrogenase) NP_214996.1 570539 D 83332 CDS NP_214997.1 15607624 887167 571710..573065 1 NC_000962.3 Rv0483, (MTCY20G9.09), len: 451 aa. Probable lprQ,conserved lipoprotein, equivalent to CAC31963.1|AL583925|ML2446 possible lipoprotein from Mycobacterium leprae (441 aa); appears longer than ML2446,so start may be further downstream. Shows also similarity with MLCL383_24|O07707 hypothetical 43.6 kDa protein from Mycobacterium leprae; and to Q49706|B1496_F2_81 (271 aa). Similar to others lipoproteins from other organisms. Also similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0116c, Rv0192, Rv1433, Rv2518c. Contains potential N-terminal signal sequence and appropriately positioned PS00013 prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LprQ 573065 lprQ 887167 lprQ Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LprQ NP_214997.1 571710 D 83332 CDS NP_214998.1 15607625 887158 complement(573046..573801) 1 NC_000962.3 Rv0484c, (MTCY20G9.10c), len: 251 aa. Probable short-chain oxidoreductase, highly similar to others e.g. T36118|4678912|CAB41284.1|AL049707 probable oxidoreductase from Streptomyces coelicolor (260 aa); YDFG_HAEIN|P45200|HI1430 hypothetical oxidoreductase (SDR family) from Haemophilus influenzae (252 aa), FASTA scores: opt: 496, E(): 7.9e-25, (35.0 % identity in 243 aa overlap); etc. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Strong similarity,to bacterial YDFG homologs.; Probable short-chain type oxidoreductase 573801 887158 Rv0484c Mycobacterium tuberculosis H37Rv Probable short-chain type oxidoreductase NP_214998.1 573046 R 83332 CDS NP_214999.1 15607626 887170 573984..575300 1 NC_000962.3 Rv0485, (MTCY20G9.11), len: 438 aa. Possible transcriptional repressor, member of the NAGC/XYLR repressor family; similar to several e.g. D87820_3|O32446|D82254 NAGC N-acetylglucosamine repressor from Vibrio cholerae (404 aa), FASTA scores: opt: 378, E(): 1.2e-17, (26.9% identity in 350 aa overlap); NAGC_ECOLI|P15301 N-acetylglucosamine repressor from Escherichia coli (406 aa), FASTA scores: opt: 305, E(): 1.8e-12, (21.8% identity in 357 aa overlap); etc.; Possible transcriptional regulatory protein 575300 887170 Rv0485 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_214999.1 573984 D 83332 CDS NP_215000.1 15607627 887160 575348..576790 1 NC_000962.3 Rv0486, (MTCY20G9.12), len: 480 aa. MshA,glycosyltransferase (see citations below), highly similar to P54138|Y486_MYCLE|ML2443 possible glycosyl transferase from Mycobacterium leprae (428 aa); and S72892|B2168_C2_201 probable hexosyltransferase from Mycobacterium leprae (409 aa), FASTA scores: opt: 2375, E(): 0, (86.4% identity in 413 aa overlap). Also highly similar to CAC04040.1|AL391406 putative transferase from Streptomyces coelicolor (496 aa); and similar to various transferases e.g. NP_437172.1|NC_003078 putative membrane-anchored glycosyltransferase protein from Sinorhizobium meliloti (416 aa); O26550|U67601_1 LPS biosynthesis related protein from Methanococcus jannaschii (411 aa), FASTA score: (25.3% identity in 387 aa overlap); etc. Also similar to CAC87824.1|AJ316594 putative sucrose-phosphate synthase from Nostoc punctiforme (422 aa). Contains a match to Pfam entry PF00534 glycosyl_transf_1 - Glycosyl transferases group 1.; Glycosyltransferase MshA 576790 mshA 887160 mshA Mycobacterium tuberculosis H37Rv Glycosyltransferase MshA NP_215000.1 575348 D 83332 CDS NP_215001.1 15607628 887162 576787..577338 1 NC_000962.3 Rv0487, (MTCY20G9.13), len: 183 aa. Conserved hypothetical protein, highly similar to P54139|Y487_MYCLE|U00018_38|ML2442 hypothetical 20.8 KDA protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 760, E(): 2.4 e-34, (73.0% identity in 159 aa overlap). Also highly similar to CAC04041.1|AL391406 conserved hypothetical protein from Streptomyces coelicolor (168 aa).; hypothetical protein 577338 887162 Rv0487 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215001.1 576787 D 83332 CDS NP_215002.1 15607629 887171 577664..578269 1 NC_000962.3 Rv0488, (MTCY20G9.14), len: 201 aa. Probable conserved integral membrane protein, LysE family possibly involved in transport of Lysine, similar to others and conserved hypothetical proteins e.g. AB93746.1|AL357613 putative membrane transport protein from Streptomyces coelicolor (204 aa); D83100|PA4365 probable transporter from Pseudomonas aeruginosa (200 aa); YGGA_ECOLI|P11667 hypothetical 21.7 kDa protein from Escherichia coli (197 aa), FASTA scores: opt: 382, E(): 1.1e-19, (39.1% identity in 179 aa overlap); CGLYSEG_2 C|P94633 lysine exporter protein (236 aa), FASTA scores: E(): 2.3e-07, (33.3% identity in 219 aa overlap). Also similar to Rv1986 from Mycobacterium tuberculosis.; Probable conserved integral membrane protein 578269 887171 Rv0488 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215002.1 577664 D 83332 CDS YP_177731.1 57116739 887183 578426..579175 1 NC_000962.3 Rv0489, (MTCY20G9.15), len: 249 aa. Probable gpm1,phosphoglycerate mutase 1, equivalent to P53531|PMGY_MYCLE phosphoglycerate mutase from Mycobacterium leprae (247 aa). Also highly similar to others e.g. PMG1_ECOLI|P31217 (249 aa), FASTA scores: opt: 805, E(): 0, (51.4% identity in 245 aa overlap); etc. Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the phosphoglycerate mutase family. Note that previously known as gpm.; Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) 579175 gpm1 887183 gpm1 Mycobacterium tuberculosis H37Rv Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) YP_177731.1 578426 D 83332 CDS NP_215004.1 15607631 887185 579349..580581 1 NC_000962.3 Rv0490, (MTCY20G9.16), len: 410 aa. Putative senX3,two-component sensor histidine kinase, transmembrane protein (see citations below), equivalent to O07129|SEX3_MYCBO sensor-like histidine kinase SENX3 from Mycobacterium bovis BCG (410 aa), FASTA scores: E(): 0,(99.5% identity in 410 aa overlap); and highly similar to P54883|SEX3_MYCLE|SENX3 sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA score: (83.8% identity in 408 aa overlap). Also highly similar, except in N-terminus, to CAC31957.1|AL583925 probable two-component system sensor histidine kinase from Mycobacterium leprae (441 aa). Also highly similar to sensor kinase proteins from other organisms e.g. CAB77323.1|AL160331 putative sensor kinase protein from Streptomyces coelicolor (426 aa).; Putative two component sensor histidine kinase SenX3 580581 senX3 887185 senX3 Mycobacterium tuberculosis H37Rv Putative two component sensor histidine kinase SenX3 NP_215004.1 579349 D 83332 CDS NP_215005.1 15607632 887195 580809..581492 1 NC_000962.3 Rv0491, (MTCY20G9.17), len: 227 aa. RegX3, response regulator protein (sensory transduction protein) (see citations below), equivalent to O07130|RGX3_MYCBO|REGX3 sensory transduction protein from Mycobacterium bovis BCG (227 aa); AAG09797.1|AF258346_2|AF258346|REGX3 response regulator from Mycobacterium smegmatis (228 aa); equivalent to P54884|RGX3_MYCLE|REGX3 sensory transduction protein from Mycobacterium leprae (198 aa), FASTA scores : E(): 0,(95.4% identity in 197 aa overlap). Also highly similar to other response regulators e.g. AAG43239.1|AF123314_2 |AF123314 putative response regulator from Corynebacterium glutamicum (232 aa).; Two component sensory transduction protein RegX3 (transcriptional regulatory protein) (probably LuxR-family) 581492 regX3 887195 regX3 Mycobacterium tuberculosis H37Rv Two component sensory transduction protein RegX3 (transcriptional regulatory protein) (probably LuxR-family) NP_215005.1 580809 D 83332 CDS NP_215006.2 57116740 887199 complement(581489..583378) 1 NC_000962.3 Rv0492c, (MT0511/MT0512, MTCY20G9.18c), len: 629 aa. Probable oxidoreductase GMC type, similar to others except in N-terminus e.g. P55582|AE000087_5|Y4NJ_RHISN hypothetical GMC-type oxidoreductase from Rhizobium sp. (505 aa), FASTA scores: opt: 873, E():0, (34.3% identity in 502 aa overlap); YTH2_RHOER|P46371 hypothetical 53.0 kDa GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); YTH2_RHOSO|P46371 hypothetical 53.0 kDa gmc-type oxidoreductase from Rhodococcus erythropolis (493 aa),FASTA score: (25.7% identity in 521 aa overlap); NP_085596.1|NC_002679 probable oxidoreductase from Mesorhizobium loti (507 aa); NP_285451.1|NC_001264 GMC oxidoreductase from Deinococcus radiodurans (722 aa); NP_249055.1|NC_002516 probable oxidoreductase from Pseudomonas aeruginosa (531 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature,and PS00624 GMC oxidoreductases signature 2. Belongs to the GMC oxidoreductases family. Cofactor: FAD (by similarity). Note that start changed since first submission (previously 684 aa).; Probable oxidoreductase GMC-type 583378 887199 Rv0492c Mycobacterium tuberculosis H37Rv Probable oxidoreductase GMC-type NP_215006.2 581489 R 83332 CDS YP_177623.1 57116741 3205069 complement(583375..583704) 1 NC_000962.3 Rv0492A, len: 109 aa. Hypothetical unknown protein. GC plot suggests CDS.; Hypothetical protein 583704 3205069 Rv0492A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177623.1 583375 R 83332 CDS NP_215007.1 15607634 887200 complement(583701..584690) 1 NC_000962.3 Rv0493c, (MTCY20G9.19), len: 329 aa. Conserved protein, showing some similarity to U00018_33|B2168_F2_93 from Mycobacterium leprae (167 aa), FASTA scores: opt: 166,E(): 0.00077, (35.9% identity in 131 aa overlap).; hypothetical protein 584690 887200 Rv0493c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215007.1 583701 R 83332 CDS NP_215008.2 57116742 887166 584695..585423 1 NC_000962.3 Rv0494, (MTCY20G9.20), len: 242 aa. Probable transcriptional regulator, GntR family, with C-terminal part highly similar to S72893|B2168_C2_205 hypothetical protein from Mycobacterium leprae (105 aa). Also similar to other transcription regulators e.g. PDHR_ECOLI|P06957 pyruvate dehydrogenase complex repressor PDHR or GENA from Escherichia coli (254 aa), FASTA scores: opt: 284, E(): 1.2e-11, (32.6% identity in 224 aa overlap); etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature, and probable helix-turn helix motif from aa 50-71 (Score 1229, +3.37 SD).; Probable transcriptional regulatory protein (probably GntR-family) 585423 887166 Rv0494 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably GntR-family) NP_215008.2 584695 D 83332 CDS NP_215009.1 15607636 887198 complement(585424..586314) 1 NC_000962.3 Rv0495c, (MTCY20G9.21c), len: 296 aa. Conserved hypothetical protein, highly similar to S72915|B2168_F1_37 hypothetical protein from Mycobacterium leprae (323 aa),FASTA scores: opt: 1615, E(): 0, (82.7% identity in 271 aa overlap); and P54579|Y495_MYCLE|ML243|13094009|CAC31952.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (277 aa). Also highly similar to Q9X8H2|Y716_STRCO|SCE7.16 hypothetical protein from Streptomyces coelicolor (271 aa).; hypothetical protein 586314 887198 Rv0495c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215009.1 585424 R 83332 CDS NP_215010.3 448824746 887234 586394..587380 1 NC_000962.3 Rv0496, (MTCY20G9.22), len: 328 aa. Conserved hypothetical protein, highly similar to S72894|467046|AAA17230.1|U00018 exopolyphosphatase ppx from Mycobacterium leprae (406 aa), FASTA scores: opt: 1902,E(): 0, (86.6% identity in 343 aa overlap); and P54882|Y496_MYCLE|ML2434|13094008|CAC31951.1|AL583925 hypothetical 36.2 KDA protein from Mycobacterium leprae (339 aa). Also highly similar to hypothetical proteins and exopolyphosphatases e.g. Q9X8H1|Y715_STRCO|SCE7.15c hypothetical protein from Streptomyces coelicolor (309 aa). C-terminal region similar to CGU31224_1|Q46054 protein similar to ppx gene product of Mycobacterium leprae from Cornybacterium glutamicum (140 aa), FASTA scores: opt: 615,E(): 2.7e-33, (70.9% identity in 134 aa overlap).; hypothetical protein 587380 887234 Rv0496 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215010.3 586394 D 83332 CDS NP_215011.1 15607638 887240 587377..588309 1 NC_000962.3 Rv0497, (MTCY20G9.23), len: 310 aa. Probable conserved transmembrane protein, equivalent (but shorter in C-terminus) to P54580|Y497_MYCLE|ML2433 hypothetical 37.9 KDA protein from Mycobacterium leprae (355 aa). N-terminus highly similar to S72922|B2168_C1_166|467074 hypothetical protein from Mycobacterium leprae (118 aa), FASTA scores: opt: 350, E(): 1.4e-12, (57.9% identity in 114 aa overlap); and hydrophobic C-terminus, highly similar to S72895|B2168_C2_209|467047 hypothetical protein from Mycobacterium leprae (241 aa), FASTA scores: opt: 473, E(): 8e-19, (53.9% identity in 241 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 588309 887240 Rv0497 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215011.1 587377 D 83332 CDS NP_215012.1 15607639 887238 588325..589167 1 NC_000962.3 Rv0498, (MTCY20G9.24), len: 280 aa. Conserved hypothetical protein, highly similar to P54581|Y498_MYCLE|ML2432 hypothetical 30.5 KDA protein from Mycobacterium leprae (280 aa); and S72896|B2168_C2_210 hypothetical protein from Mycobacterium leprae (244 aa),FASTA scores: opt: 1486, E():0, (89.3% identity in 244 aa overlap). Also similar to Q9X8H0|Y714_STRCO|SCE7.14c hypothetical protein from Streptomyces coelicolor.; hypothetical protein 589167 887238 Rv0498 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215012.1 588325 D 83332 CDS NP_215013.1 15607640 887243 589183..590058 1 NC_000962.3 Rv0499, (MTCY20G9.25), len: 291 aa. Conserved hypothetical protein, showing some similarity to AL031184|SC2A11_16|T34762 hypothetical protein from Streptomyces coelicolor (340 aa), FASTA scores: opt: 240,E(): 1.8e-07, (28.9% identity in 270 aa overlap).; hypothetical protein 590058 887243 Rv0499 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215013.1 589183 D 83332 CDS NP_215014.1 15607641 887256 590083..590970 1 NC_000962.3 Rv0500, (MTCY20G9.26), len: 295 aa. Probable proC,Pyrroline-5-carboxylate reductase (see citation below),equivalent to P46725|PROC_MYCLE pyrroline-5-carboxylate reductase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1473, E(): 0, (82.4% identity in 295 aa overlap). Also similar to others e.g. P46540|PROC_CORGL pyrroline-5-carboxylate reductase from Corynebacterium glutamicum (270 aa); T36286|4803683|CAB42663.1|AL049819 pyrroline-5-carboxylate reductase from Streptomyces coelicolor (284 aa); etc. Belongs to the pyrroline-5-carboxylate reductase family.; Probable pyrroline-5-carboxylate reductase ProC (P5CR) (P5C reductase) 590970 proC 887256 proC Mycobacterium tuberculosis H37Rv Probable pyrroline-5-carboxylate reductase ProC (P5CR) (P5C reductase) NP_215014.1 590083 D 83332 CDS YP_177624.1 57116743 3205035 591111..591347 1 NC_000962.3 Rv0500A, len: 78 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. U00018_25 from Mycobacterium leprae cosmid B2168 (86 aa), FASTA scores: opt: 428, E(): 1.3e-27, (82.6% identity in 86 aa overlap); AL079345|SCE68_26 from Streptomyces coelicolor cosmid E6 (70 aa), FASTA scores: opt: 252, E(): 1.2 e-13, (72.2 identity in 54 aa overlap).; hypothetical protein 591347 3205035 Rv0500A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177624.1 591111 D 83332 CDS YP_177625.1 57116744 3205036 591475..591576 1 NC_000962.3 Rv0500B, len: 33 aa. Conserved hypothetical protein. Basic protein 18 of the 33 aa are Arg or Lys, with strong similarity to AL079345|SCE68_25 protein from Streptomyces coelicolor cosmid E6 (32 aa), FASTA scores: opt: 176, E(): 1e-06, (93.1% identity in 29 aa overlap). Same gene arrangement in both actinomycetes.; hypothetical protein 591576 3205036 Rv0500B Mycobacterium tuberculosis H37Rv hypothetical protein YP_177625.1 591475 D 83332 CDS NP_215050.2 57116745 887228 591654..592784 1 NC_000962.3 Rv0501, (MTCY20G9.28), len: 376 aa. Possible galE2,UDP-glucose 4-epimerase, highly similar (except in N-terminus) to CAC31944.1|AL583925 possible glucose epimerase/dehydratase from Mycobacterium leprae (364 aa). N-terminus highly similar to S72923|B2168_C1_174|467075|AAA17259.1|U00018 hypothetical protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 934, E(): 0, (89.6% identity in 164 aa overlap); and C-terminus highly similar to S72898|467050|AAA17234.1|U00018 hypothetical protein from Mycobacterium leprae (168 aa), FASTA scores: opt: 928, E(): 0, (82.7% identity in 168 aa overlap). Also highly similar to T36274|5123671|CAB45360.1|AL079345 probable epimerase from Streptomyces coelicolor (353 aa); and similar in part to other epimerases e.g. GALE_ECOLI|P09147 UDP-glucose 4-epimerase from Escherichia coli (338 aa), FASTA scores: opt: 241, E(): 6.7e-09, (28.2% identity in 294 aa overlap); etc. Belongs to the sugar epimerase family. Cofactor: NAD. Note that previously known as galE1.; Possible UDP-glucose 4-epimerase GalE2 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) 592784 galE2 887228 galE2 Mycobacterium tuberculosis H37Rv Possible UDP-glucose 4-epimerase GalE2 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) NP_215050.2 591654 D 83332 CDS NP_215016.1 15607643 887260 592791..593867 1 NC_000962.3 Rv0502, (MTCY20G9.29), len: 358 aa. Conserved protein, equivalent to P54878|Y502_MYCLE|ML2427 hypothetical 40.5 KDA protein from Mycobacterium leprae (367 aa), FASTA scores: opt: 2042, E(): 0, (84.1% identity in 365 aa overlap). Also similar to T36273|SCE68.23c hypothetical protein from Streptomyces coelicolor (355 aa). C-terminal similar to AL021529|SC10A5_4|T34572 hypothetical protein from Streptomyces coelicolor (295 aa), FASTA score: (57.8% identity in 263 aa overlap); and to hypothetical proteins from Mycobacterium tuberculosis Rv1920|G70808 (287 aa); and Rv1428c|G70914 (275 aa).; hypothetical protein 593867 887260 Rv0502 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215016.1 592791 D 83332 CDS NP_215017.1 15607644 887264 complement(593871..594779) 1 NC_000962.3 Rv0503c, (MTCY20G9.30c), len: 302 aa. CmaA2 (alternate gene name: cma2),cyclopropane-fatty-acyl-phospholipid synthase 2 (mycolic acid trans-cyclopropane synthetase) (see citations below). Note that this protein has 302 aa and not 322 aa: we have chosen a different initiation codon on the basis of homology. Equivalent to S72886|B2168_F3_130 hypothetical protein from Mycobacterium leprae (308 aa), FASTA score: (78.9% identity in 303 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also similar to other proteins from Mycobacterium tuberculosis and Mycobacterium bovis BCG e.g. MTV038_14|UMAA2|Rv0470c|MTV038.14 putative mycolic acid synthesis/modification protein (287 aa) (57.2% identity in 297 aa overlap).; Cyclopropane-fatty-acyl-phospholipid synthase 2 CmaA2 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 2) (mycolic acid trans-cyclopropane synthetase) 594779 cmaA2 887264 cmaA2 Mycobacterium tuberculosis H37Rv Cyclopropane-fatty-acyl-phospholipid synthase 2 CmaA2 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 2) (mycolic acid trans-cyclopropane synthetase) NP_215017.1 593871 R 83332 CDS NP_215018.1 15607645 887268 complement(594802..595302) 1 NC_000962.3 Rv0504c, (MTCY20G9.31c), len: 166 aa. Conserved protein, equivalent to P54879|Y504_MYCLE|ML2425 hypothetical 18.7 KDA protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 884, E(): 0, (83.1% identity in 166 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). Also similar to M. tuberculosis hypothetical proteins Rv0635|H70612 (158 aa); and Rv0637|B70613 (166 aa).; hypothetical protein 595302 887268 Rv0504c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215018.1 594802 R 83332 CDS YP_177732.1 57116746 887270 complement(595464..596585) 1 NC_000962.3 Rv0505c, (MTCY20G9.32c), len: 373 aa. Possible serB1, phosphoserine phosphatase, equivalent (but longer ~70 aa in N-terminus) to S72914|serB phosphoserine phosphatase from Mycobacterium leprae (300 aa), FASTA scores: opt: 1570, E(): 0, (83.0% identity in 306 aa overlap). C-terminus highly similar to CAB55344.1|AJ010584 phosphoserine phosphatase from Streptomyces coelicolor (266 aa). Low similarity to SERB_ECOLI|P06862 phosphoserine phosphatase from Escherichia coli strains K12 and O157:H7 (322 aa), FASTA scores: opt: 148, E(): 0.043, (24.0% identity in 150 aa overlap). C-terminus is also similar to O33611|AB004855_1|IMD_STRCN protein involved in inhibition of morphological differentiation from Streptomyces cyaneus (277 aa), FASTA score: (37.7% identity in 252 aa overlap). Seems to belong to the SERB family. Note that previously known as serB.; Possible phosphoserine phosphatase SerB1 (PSP) (O-phosphoserine phosphohydrolase) (pspase) 596585 serB1 887270 serB1 Mycobacterium tuberculosis H37Rv Possible phosphoserine phosphatase SerB1 (PSP) (O-phosphoserine phosphohydrolase) (pspase) YP_177732.1 595464 R 83332 CDS NP_215020.1 15607647 887279 596759..597202 1 NC_000962.3 Rv0506, (MTCY20G9.33), len: 147 aa. Probable mmpS2,conserved membrane protein (see citation below), highly similar to other Mycobacterial proteins e.g. C-terminus of AAD44232.1|AF143772_38|AF143772|TmtpA from Mycobacterium avium (221 aa); P54880|MMS4_MYCLE|MMPS4 putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 392, E(): 1.3e-20, (43.7% identity in 151 aa overlap); and the putative membrane proteins from Mycobacterium tuberculosis MTV040_5, MTCY4D9_16, MTV037_15. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein MmpS2 597202 mmpS2 887279 mmpS2 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein MmpS2 NP_215020.1 596759 D 83332 CDS NP_215021.1 15607648 887248 597199..600105 1 NC_000962.3 Rv0507, (MTCY20G9.34), len: 968 aa. Probable mmpL2,conserved transmembrane transport protein (see citations below), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCLE from Mycobacterium leprae (959 aa), FASTA scores: opt: 3699, E(): 0, (58.3% identity in 940 aa overlap); and the Mycobacterium tuberculosis proteins MTV037_14, MTV040_4, MTCY98_8,MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to STMACTII_3|SC10A5_9 from Streptomyces coelicolor; and BSUB0|004_12 from Bacillus subtilis. C-terminal half similar to Q50086|U1740AB from Mycobacterium leprae (386 aa), FASTA scores: opt: 1526,E(): 0, (61.5% identity in 371 aa overlap). Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL2 600105 mmpL2 887248 mmpL2 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL2 NP_215021.1 597199 D 83332 CDS NP_215022.1 15607649 887301 600098..600391 1 NC_000962.3 Rv0508, (MTCY20G9.35), len: 97 aa. Conserved hypothetical protein, showing similarity with T36269|5123666|CAB45355.1|AL079345 probable redoxin from Streptomyces coelicolor (101 aa), FASTA scores: opt: 160,E(): 3.4e-05, (33.3% identity in 75 aa overlap); and E81943|NMA0966 probable thioredoxin from Neisseria meningitidis group a strain Z2491 (77 aa).; hypothetical protein 600391 887301 Rv0508 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215022.1 600098 D 83332 CDS NP_215023.1 15607650 887292 600441..601847 1 NC_000962.3 Rv0509, (MTCY20G9.36), len: 468 aa. Probable hemA,glutamyl-tRNA reductase, equivalent to HEM1_MYCLE|P46724 glutamyl-tRNA reductase from Mycobacterium leprae (467 aa),FASTA scores: opt: 2377, E(): 0, (82.3% identity in 463 aa overlap). Also highly similar (sometimes in part) to others e.g. Q9WX15|HEM1_STRCO glutamyl-tRNA reductase from Streptomyces coelicolor (581 aa); P16618|HEM1_BACSU|HEMA glutamyl-tRNA reductase from Bacillus subtilis (455 aa); etc. Contains PS00747 Glutamyl-tRNA reductase signature. Belongs to the glutamyl-tRNA reductase family.; Probable glutamyl-tRNA reductase HemA (GLUTR) 601847 hemA 887292 hemA Mycobacterium tuberculosis H37Rv Probable glutamyl-tRNA reductase HemA (GLUTR) NP_215023.1 600441 D 83332 CDS NP_215024.1 15607651 887306 601857..602786 1 NC_000962.3 Rv0510, (MTCY21C8.01-MTCY20G9.37), len: 309 aa. Probable hemC, hydroxymethylbilane synthase (porphobilinogen deaminase), equivalent to HEM3B|Q49808|HEM3_MYCLE porphobilinogen deaminase from Mycobacterium leprae (315 aa), FASTA scores: opt: 889, E(): 0, (88.1% identity in 159 aa overlap). Also highly similar to others e.g. Q9WX16|HE31_STRCO probable porphobilinogen deaminase from Streptomyces coelicolor (319 aa); Q9L6Q2|HEM3_SALTY porphobilinogen deaminase from Salmonella typhimurium (313 aa); etc. Belongs to the HMBS family. Cofactor: covalently binds a dipyrromethane cofactor to which the porphobilinogen subunits are ADDED.; Probable porphobilinogen deaminase HemC (PBG) (hydroxymethylbilane synthase) (HMBS) (pre-uroporphyrinogen synthase) 602786 hemC 887306 hemC Mycobacterium tuberculosis H37Rv Probable porphobilinogen deaminase HemC (PBG) (hydroxymethylbilane synthase) (HMBS) (pre-uroporphyrinogen synthase) NP_215024.1 601857 D 83332 CDS YP_177733.1 57116747 887280 602819..604516 1 NC_000962.3 Rv0511, (MTCY21C8.02), len: 565 aa. Probable hemD (alternate gene name: cysG), uroporphyrin-III C-methyltransferase, highly similar to others e.g. CAC31936.1|AL583925 possible uroporphyrin-III C-methyltransferase from Mycobacterium leprae (563 aa); and S72909|CYSG from Mycobacterium leprae (472 aa), FASTA scores: opt: 1946, E(): 0, (83.3% identity in 472 aa overlap); T36265|5123662|CAB45351.1|AL079345 probable uroporphyrin-III C-methyltransferase from Streptomyces coelicolor (565 aa); and similar to others e.g. AAK00606.1|AF221100_3|AF221100 from Selenomonas ruminantium subsp. ruminantium (505 aa); etc. Also similar to Rv2071c and Rv2847c from Mycobacterium tuberculosis. Note that previously known as cysG.; Probable uroporphyrin-III C-methyltransferase HemD (uroporphyrinogen III methylase) (urogen III methylase) (SUMT) (urogen III methylase) (UROM) 604516 hemD 887280 hemD Mycobacterium tuberculosis H37Rv Probable uroporphyrin-III C-methyltransferase HemD (uroporphyrinogen III methylase) (urogen III methylase) (SUMT) (urogen III methylase) (UROM) YP_177733.1 602819 D 83332 CDS NP_215026.1 15607653 887312 604602..605591 1 NC_000962.3 Rv0512, (MTCY20G10.02), len: 329 aa. Probable hemB,delta-aminolevulinic acid dehydratase, equivalent to 46723|HEM2_MYCLE delta-aminolevulinic acid dehydratase from Mycobacterium leprae (329 aa). Also highly similar to many e.g. P54919|HEM2_STRCO from Streptomyces coelicolor (330 aa); HEM2_ECOLI|P15002 from Escherichia coli (323 aa),FASTA scores: opt: 942, E(): 0, (47.6% identity in 317 aa overlap); etc. Contains PS00169 Delta-aminolevulinic acid dehydratase active site. Belongs to the ALADH family. Cofactor: zinc.; Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH) 605591 hemB 887312 hemB Mycobacterium tuberculosis H37Rv Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH) NP_215026.1 604602 D 83332 CDS NP_215027.1 15607654 887307 605604..606152 1 NC_000962.3 Rv0513, (MTCY20G10.03), len: 182 aa. Possible conserved transmembrane protein, with its N-terminus highly similar to S72925|B2168_C1_182 hypothetical protein from Mycobacterium leprae (103 aa), FASTA scores: opt: 217, E(): 8.2e-14, (45.3 % identity in 106 aa overlap).; Possible conserved transmembrane protein 606152 887307 Rv0513 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_215027.1 605604 D 83332 CDS NP_215028.1 15607655 887319 606149..606448 1 NC_000962.3 Rv0514, (MTCY20G10.04), len: 99 aa. Possible transmembrane protein.; Possible transmembrane protein 606448 887319 Rv0514 Mycobacterium tuberculosis H37Rv Possible transmembrane protein NP_215028.1 606149 D 83332 CDS NP_215029.1 15607656 887322 606551..608062 1 NC_000962.3 Rv0515, (MTCY20G10.05), len: 503 aa. Part of M. tuberculosis 13E12 repeat family. Almost identical to Rv0336 (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other M. tuberculosis hypothetical 13E12 repeat proteins e.g. Rv1148c, Rv1945, etc.; Conserved 13E12 repeat family protein 608062 887322 Rv0515 Mycobacterium tuberculosis H37Rv Conserved 13E12 repeat family protein NP_215029.1 606551 D 83332 CDS NP_215030.1 15607657 887324 complement(608059..608535) 1 NC_000962.3 Rv0516c, (MTCY20G10.06c), len: 158 aa. Possible anti-anti-sigma factor, showing some similarity to Rv1365c|MTCY02B10_29 from Mycobacterium tuberculosis (128 aa), FASTA scores: E(): 0.0012, (27.4% identity in 124 aa overlap).; Possible anti-anti-sigma factor 608535 887324 Rv0516c Mycobacterium tuberculosis H37Rv Possible anti-anti-sigma factor NP_215030.1 608059 R 83332 CDS NP_215031.1 15607658 887323 608746..610056 1 NC_000962.3 Rv0517, (MTCY20G10.07), len: 436 aa. Possible acyltransferase, integral membrane protein, equivalent (but longer 26 aa in N-terminus) to AAK44761.1|AE006954 putative acyltransferase from Mycobacterium tuberculosis strain CDC1551 (410 aa). Also similar to many acyltransferases e.g. MDMB_STRMY|Q00718 from Streptomyces mycarofaciens (387 aa), FASTA scores: opt: 200, E(): 1.1e-08, (28.2% identity in 394 aa overlap). And similar to Rv0111, Rv0228, Rv1254,Rv1565c from Mycobacterium tuberculosis.; Possible membrane acyltransferase 610056 887323 Rv0517 Mycobacterium tuberculosis H37Rv Possible membrane acyltransferase NP_215031.1 608746 D 83332 CDS NP_215032.1 15607659 887321 610188..610883 1 NC_000962.3 Rv0518, (MTCY20G10.08), len: 231 aa. Possible exported protein; has hydrophobic N-terminus.; Possible exported protein 610883 887321 Rv0518 Mycobacterium tuberculosis H37Rv Possible exported protein NP_215032.1 610188 D 83332 CDS NP_215033.1 15607660 887334 complement(611172..612074) 1 NC_000962.3 Rv0519c, (MTCY20G10.09c), len: 300 aa. Possible conserved membrane protein, with hydrophobic region near N-terminus. Could be a lipase. Similar to Rv0774c|MTCY369.19c|A70708 from Mycobacterium tuberculosis (312 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00120 Lipases, serine active site.; Possible conserved membrane protein 612074 887334 Rv0519c Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_215033.1 611172 R 83332 CDS NP_215034.1 15607661 887331 612255..612605 1 NC_000962.3 Rv0520, (MTCY20G10.10), len: 116 aa. Possible fragment of methyltransferase (possibly first part), highly similar to part of several methyltransferases e.g. Q43445|U43683 S-adenosyl-L-methionine:DELTA24-sterol-C-methyltransferase from Glycine max (Soybean)(367 aa), FASTA scores: opt: 190,E(): 2.3e-12, (39.2% identity in 74 aa overlap). Also some similarity to MTCY19G5_5 from Mycobacterium tuberculosis. Possibly continues as Rv0521 but we can find no frameshift to account for this.; Possible methyltransferase/methylase (fragment) 612605 887331 Rv0520 Mycobacterium tuberculosis H37Rv Possible methyltransferase/methylase (fragment) NP_215034.1 612255 D 83332 CDS YP_177626.1 57116748 3205045 612598..612903 1 NC_000962.3 Rv0521, (replaces MTCY20G10.11), len: 101 aa. Possible fragment of methyltransferase (possibly second part), highly similar to C-terminus of several methyltransferases e.g. AAF87203.1|AF216282 sarcosine-dimethylglycine methyltransferase from Halorhodospira halochloris (279 aa). Possibly continuation of Rv0520 but we can find no frameshift to account for this.; Possible methyltransferase/methylase (fragment) 612903 3205045 Rv0521 Mycobacterium tuberculosis H37Rv Possible methyltransferase/methylase (fragment) YP_177626.1 612598 D 83332 CDS YP_177734.1 57116749 886261 613038..614342 1 NC_000962.3 Rv0522, (MTCY20G10.12), len: 434 aa. Probable gabP,GABA permease (gamma-aminobutyrate permease), integral membrane protein, highly similar to others e.g. GABP_ECOLI|P25527 gaba permease from Escherichia coli (466 aa), FASTA scores: opt: 1218, E(): 0, (44.3% identity in 424 aa overlap); etc. Also similar to other M. tuberculosis permeases e.g. MTCY13E10.06c FASTA score: (34.4% identity in 407 aa overlap). Contains PS00218 Amino acid permeases signature. Overlaps and extends Rv0523c|MTCY25D10.01 from overlapping cosmid. Belongs to the amino acid permease family (APC family).; Probable GABA permease GabP (4-amino butyrate transport carrier) (GAMA-aminobutyrate permease) 614342 gabP 886261 gabP Mycobacterium tuberculosis H37Rv Probable GABA permease GabP (4-amino butyrate transport carrier) (GAMA-aminobutyrate permease) YP_177734.1 613038 D 83332 CDS NP_215037.1 15607663 887341 complement(614326..614721) 1 NC_000962.3 Rv0523c, (MTCY25D10.02), len: 131 aa. Conserved protein, showing some similarity to M. tuberculosis proteins Rv1598c|MTCY336.06; and Rv1871c|MTCY336_06|O06592 (136 aa), FASTA scores: opt: 197, E(): 5e-08, (38.4% identity in 99 aa overlap).; hypothetical protein 614721 887341 Rv0523c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215037.1 614326 R 83332 CDS NP_215038.1 15607664 887349 614835..616223 1 NC_000962.3 Rv0524, (MTCY25D10.03), len: 462 aa. Probable hemL,glutamate-1-semialdehyde 2,1-aminomutase, equivalent to P46716|GSA_MYCLE glutamate-1-semialdehyde 2,1-aminomutase from Mycobacterium leprae (446 aa), FASTA scores: opt: 1532, E(): 0, (82.6% identity in 460 aa overlap). Also highly similar to others e.g. Q9F2S0|GSA_STRCO from Streptomyces coelicolor (438 aa); Q06774|GSA_PROFR from Propionibacterium freudenreichii (441 aa); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate.; Probable glutamate-1-semialdehyde 2,1-aminomutase HemL (GSA) (glutamate-1-semialdehyde aminotransferase) (GSA-at) 616223 hemL 887349 hemL Mycobacterium tuberculosis H37Rv Probable glutamate-1-semialdehyde 2,1-aminomutase HemL (GSA) (glutamate-1-semialdehyde aminotransferase) (GSA-at) NP_215038.1 614835 D 83332 CDS NP_215039.1 15607665 887358 616223..616831 1 NC_000962.3 Rv0525, (MTCY25D10.04), len: 202 aa. Conserved protein, equivalent to Q49821|B2168_C3_276|S72912 hypothetical protein from Mycobacterium leprae (202 aa),FASTA scores: opt: 1151, E(): 0, (82.5% identity in 200 aa overlap). Also highly similar to CAC08377.1|AL392176 putative phosphoglycerate mutase from Streptomyces coelicolor (233 aa); and similar to SLL0395|Q55734 hypothetical 23.8 kDa protein from synechocystis SP. (212 aa), FASTA scores: opt: 207, E(): 5.1e-07, (28.2% identity in 195 aa overlap). Also some similarity to Rv2228c|Y019_MYCTU|Q10512|cy427.09 hypothetical 39.2 kDa protein from Mycobacterium tuberculosis (364 aa), FASTA scores: opt: 236, E(): 1.1e-08, (34.3% identity in 198 aa overlap).; hypothetical protein 616831 887358 Rv0525 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215039.1 616223 D 83332 CDS NP_215040.1 15607666 887339 616846..617496 1 NC_000962.3 Rv0526, (MTCY25D10.05), len: 216 aa. Possible thioredoxin protein (thiol-disulfide interchange protein) ,equivalent to Q49816|U2168C|S72901 hypothetical protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1144, E(): 0, (78.5% identity in 214 aa overlap). C-terminus shows some similarity to C-terminus of thioredoxins e.g. RESA_BACSU|P35160 resa protein from Bacillus subtilis (181 aa), FASTA scores: opt: 200, E(): 7.4e-06, (24.2% identity in 132 aa overlap); etc. Also similar to Mycobacterium tuberculosis thioredoxin-like proteins Rv1470, Rv1471, Rv1677, etc. Contains PS00194 Thioredoxin family active site. Seems to belong to the thioredoxin family.; Possible thioredoxin protein (thiol-disulfide interchange protein) 617496 887339 Rv0526 Mycobacterium tuberculosis H37Rv Possible thioredoxin protein (thiol-disulfide interchange protein) NP_215040.1 616846 D 83332 CDS YP_177735.1 57116750 887362 617493..618272 1 NC_000962.3 Rv0527, (MTCY25D10.06), len: 259 aa. Possible ccdA,cytochrome C-type biogenesis protein, integral membrane protein, equivalent to Q49810|B2168_C1_192|S72890 hypothetical protein from Mycobacterium leprae (262 aa),FASTA scores: opt: 1341, E(): 0, (79.0% identity in 262 aa overlap). Also highly similar to others e.g. CAC08380.1 (253 aa); CCDA_BACSU|P45706 cytochrome C-type biogenesis protein from Bacillus subtilis (235 aa), FASTA scores: opt: 307, E(): 7.4e-13, (30.4% identity in 237 aa overlap); etc. Seems to belong to the DSBD subfamily. Note that previously known as ccsA.; Possible cytochrome C-type biogenesis protein CcdA 618272 ccdA 887362 ccdA Mycobacterium tuberculosis H37Rv Possible cytochrome C-type biogenesis protein CcdA YP_177735.1 617493 D 83332 CDS NP_215042.1 15607668 887372 618305..619894 1 NC_000962.3 Rv0528, (MTCY25D10.07), len: 529 aa. Probable conserved transmembrane protein, equivalent (shorter 14 aa in N-terminus) to CAC31926.1|AL583925 conserved membrane protein from Mycobacterium leprae (542 aa). Also highly similar to Q49817|B2168_C2_237|S72902 hypothetical protein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1846, E(): 0, (81.1% identity in 338 aa overlap); and Q49811|B2168_C1_194|S72891 hypothetical protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 506, E(): 3.8e-26, (73.6% identity in 106 aa overlap). Also highly similar to CAC08381.1|AL392176 putative integral membrane protein from Streptomyces coelicolor (574 aa).; Probable conserved transmembrane protein 619894 887372 Rv0528 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215042.1 618305 D 83332 CDS NP_215041.2 57116751 887380 619891..620865 1 NC_000962.3 Rv0529, (MTCY25D10.08), len: 324 aa. Possible ccsA,cytochrome C-type biogenesis protein, integral membrane protein, equivalent to NP_302558.1|NC_002677|B2168_C3_281 possible cytochrome C biogenesis protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1779, E(): 0, (82.9% identity in 327 aa overlap). Also highly similar to others e.g. CAC08382.1|AL392176 putative cytochrome biogenesis related protein from Streptomyces coelicolor (380 aa); CCSA_CHLRE|P48269 probable cytochrome c biogenesis protein from Chlamydomonas reinhardtii (353 aa), FASTA scores: opt: 449, E(): 1.3e-23, (34.4% identity in 247 aa overlap); etc. Belongs to the CCMF/CYCK/CCL1/NRFE/CCSA family. Note that previously known as ccsB.; Possible cytochrome C-type biogenesis protein CcsA 620865 ccsA 887380 ccsA Mycobacterium tuberculosis H37Rv Possible cytochrome C-type biogenesis protein CcsA NP_215041.2 619891 D 83332 CDS NP_215044.1 15607670 887385 620907..622124 1 NC_000962.3 Rv0530, (MTCY25D10.09), len: 405 aa. Conserved protein, similar in part to other hypothetical proteins e.g. AL031231|SC3C3_3|CAA20252.1 from Streptomyces coelicolor (1083 aa), FASTA scores: opt: 870, E(): 0,(39.5% identity in 443 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3868, Rv0282,Rv1798, etc.; hypothetical protein 622124 887385 Rv0530 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215044.1 620907 D 83332 CDS YP_007409181.1 448815308 14515848 complement(622121..622282) 1 NC_000962.3 Rv0530A, len: 53 aa. Conserved protein.; hypothetical protein 622282 14515848 Rv0530A Mycobacterium tuberculosis H37Rv hypothetical protein YP_007409181.1 622121 R 83332 CDS NP_215045.1 15607671 887376 622329..622646 1 NC_000962.3 Rv0531, (MTCY25D10.10), len: 105 aa. Possible conserved membrane protein, highly similar to Y13803|MLB1306_1|CAA74131.1 hypothetical protein from Mycobacterium leprae (86 aa), FASTA scores: E(): 2.1e-24,(74.4% identity in 86 aa overlap); and NP_302557.1|NC_002677 putative membrane protein from Mycobacterium leprae (111 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Possible conserved membrane protein 622646 887376 Rv0531 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_215045.1 622329 D 83332 CDS YP_177736.1 57116752 887391 622793..624577 1 NC_000962.3 Rv0532, (MTCY25D10.11), len: 594 aa. PE_PGRS6,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below),similar to others e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1703, E(): 0,(58.2% identity in 536 aa overlap).; PE-PGRS family protein PE_PGRS6 624577 PE_PGRS6 887391 PE_PGRS6 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS6 YP_177736.1 622793 D 83332 CDS NP_215047.1 15607673 887381 complement(624473..625480) 1 NC_000962.3 Rv0533c, (MTCY25D10.12c), len: 335 aa. FabH (alternate gene name: mtFabH), 3-oxoacyl-[acyl-carrier protein] synthase III (see citations below), highly similar to others e.g. Q54206|FABH from streptomyces glaucescens (333 aa), FASTA scores: opt: 1109, E(): 0, (51.4% identity in 333 aa overlap); FABH_ECOLI|P24249 3-oxoacyl-[acyl-carrier-protein] synthase III (317 aa),FASTA scores: opt: 666, E(): 0, (37.1% identity in 318 aa overlap); etc. Belongs to the FabH family.; 3-oxoacyl-[acyl-carrier-protein] synthase III FabH (beta-ketoacyl-ACP synthase III) (KAS III) 625480 fabH 887381 fabH Mycobacterium tuberculosis H37Rv 3-oxoacyl-[acyl-carrier-protein] synthase III FabH (beta-ketoacyl-ACP synthase III) (KAS III) NP_215047.1 624473 R 83332 CDS NP_215048.1 15607674 887408 complement(625562..626440) 1 NC_000962.3 Rv0534c, (MTCY25D10.13c), len: 292 aa. Probable menA, 1,4-dihydroxy-2-naphthoate octaprenyltransferase,integral membrane protein, equivalent to Y13803|MLB1306_2|NP_302556.1 probable 4-dihydroxy-2-naphthoate octaprenyltransferase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1509,E(): 0, (80.2% identity in 288 aa overlap). Also highly similar to others e.g. MENA_ECOLI|P32166|B3930 from Escherichia coli (308 aa), FASTA scores: opt: 495, E(): 2.9e-25, (36.3 identity in 289 aa overlap); etc. Belongs to the MenA family.; 1,4-dihydroxy-2-naphthoate octaprenyltransferase MenA (DHNA-octaprenyltransferase) 626440 menA 887408 menA Mycobacterium tuberculosis H37Rv 1,4-dihydroxy-2-naphthoate octaprenyltransferase MenA (DHNA-octaprenyltransferase) NP_215048.1 625562 R 83332 CDS NP_215049.1 15607675 887430 626457..627251 1 NC_000962.3 Rv0535, (MTCY25D10.14c), len: 264 aa. Probable pnp,5'-methylthioadenosine phosphorylase, highly similar to others e.g. CAB90972.1|AL355832 putative methylthioadenosine phosphorylase from Streptomyces coelicolor (280 aa); etc. Also similar to Rv3307|deoD probable purine nucleoside phosphorylase from Mycobacterium tuberculosis (268 aa). Belongs to the PNP/MTAP family 2 of phosphorylases. Gene name could be inappropriate.; Probable 5'-methylthioadenosine phosphorylase Pnp (MTA phosphorylase) 627251 pnp 887430 pnp Mycobacterium tuberculosis H37Rv Probable 5'-methylthioadenosine phosphorylase Pnp (MTA phosphorylase) NP_215049.1 626457 D 83332 CDS YP_177737.1 57116753 887457 627248..628288 1 NC_000962.3 Rv0536, (MTCY25D10.15), len: 346 aa. Possible galE3,UDP-glucose 4-epimerase, highly similar to CAB76986.1|AL159178 putative epimerase from Streptomyces coelicolor (334 aa); and similar to other epimerases e.g. NP_436775.1|NC_003078 putative NDP-glucose dehydrataseepimerase protein from Sinorhizobium meliloti (368 aa); AF143772|AF143772_7 GepiA from Mycobacterium avium strain 2151 (353 aa), FASTA scores: opt: 577, E(): 3.9e-29, (36.6% identity in 352 aa overlap); GALE_METJA|Q57664 putative UDP-glucose 4-epimerase (305 aa), FASTA scores: opt: 300, E(): 1.6e-12, (30.9% identity in 343 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3634c, Rv3784, etc. Seems to belong to the sugar epimerase family. Note that previously known as galE2.; Probable UDP-glucose 4-epimerase GalE3 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) 628288 galE3 887457 galE3 Mycobacterium tuberculosis H37Rv Probable UDP-glucose 4-epimerase GalE3 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) YP_177737.1 627248 D 83332 CDS NP_215051.1 15607677 887465 complement(628298..629731) 1 NC_000962.3 Rv0537c, (MTCY25D10.16c), len: 477 aa. Probable integral membrane protein, showing weak similarity to YDNK_STRCO|P40180 hypothetical 41.2 kDa protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 122,E(): 0.85, (28.2% identity in 373 aa overlap).; Probable integral membrane protein 629731 887465 Rv0537c Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_215051.1 628298 R 83332 CDS NP_215052.1 15607678 887473 630040..631686 1 NC_000962.3 Rv0538, (MTCY25D10.17), len: 548 aa. Possible conserved membrane protein. Middle region highly similar to AAB63811.1|AF009829|MBE4863a|O32850 unknown protein from Mycobacterium bovis (295 aa) possible transmembrane protein with a repetitive proline, threonine-rich region at C-terminus.; Possible conserved membrane protein 631686 887473 Rv0538 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_215052.1 630040 D 83332 CDS NP_215053.1 15607679 887476 631743..632375 1 NC_000962.3 Rv0539, (MTCY25D10.18), len: 210 aa. Probable dolichol-P-sugar synthase, highly similar to CAB76989.1|AL159178 putative glycosyltransferase from Streptomyces coelicolor (242 aa), and similar to various dolichol-P-sugar synthetases and sugar transferases e.g. NP_126257.1|NC_000868 dolichyl-phosphate mannose synthase related protein from Pyrococcus abyssi (211 aa); N-terminus of NP_127133.1|NC_000868 dolichol-P-glucose synthetase from Pyrococcus abyssi (378 aa); N-terminus of NP_068880.1|NC_000917 putative dolichol-P-glucose synthetase from Archaeoglobus fulgidus (369 aa), FASTA scores: E(): 2.4e-13, (32. 1% identity in 193 aa overlap); Q26732 dolichyl-phosphate-mannose synthase precursor from trypanosoma brucei (267 aa), FASTA scores: opt: 179, E(): 0.0011, (30.7% identity in 205 aa overlap); etc. Also similar to Rv2051c|MTY25D10_18 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable dolichyl-phosphate sugar synthase (dolichol-phosphate sugar synthetase) (dolichol-phosphate sugar transferase) (sugar phosphoryldolichol synthase) 632375 887476 Rv0539 Mycobacterium tuberculosis H37Rv Probable dolichyl-phosphate sugar synthase (dolichol-phosphate sugar synthetase) (dolichol-phosphate sugar transferase) (sugar phosphoryldolichol synthase) NP_215053.1 631743 D 83332 CDS NP_215054.1 15607680 887489 632372..633034 1 NC_000962.3 Rv0540, (MTCY25D10.19), len: 220 aa. Conserved hypothetical protein, similar to hypothetical proteins from Streptomyces coelicolor: CAB76990.1|AL159178 (213 aa); N-terminus of BAA84086.1|AB032065 (446 aa); and CAB61872.1|AL133252|SCE46_21 (210 aa), FASTA scores: opt: 267, E(): 5.3e-10, (32.7% identity in 202 aa overlap). Also some similarity with D90913_63|PCC6803 from Synecho cystis sp (211 aa), FASTA scores: opt: 189, E(): 4.7e-06, (25.3 identity in 194 aa overlap).; hypothetical protein 633034 887489 Rv0540 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215054.1 632372 D 83332 CDS NP_215055.1 15607681 887416 complement(633055..634404) 1 NC_000962.3 Rv0541c, (MTCY25D10.20c), len: 449 aa. Probable conserved integral membrane protein, highly similar (except first 40 residues) to CAB76994.1|AL159178 putative integral membrane protein from Streptomyces coelicolor (456 aa). Also some similarity to Q13724|GCS1_HUMAN mannosyl-oligosaccharide glucosidase (834 aa), FASTA scores: opt: 150, E(): 0.013, (27.1% identity in 339 aa overlap). Contains PS00041 Bacterial regulatory proteins,araC family signature.; Probable conserved integral membrane protein 634404 887416 Rv0541c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215055.1 633055 R 83332 CDS NP_215056.1 15607682 887507 complement(634416..635504) 1 NC_000962.3 Rv0542c, (MTCY25D10.21c), len: 362 aa. Possible menE, O-succinylbenzoic acid-CoA ligase, highly similar to Q50170|AAA63145.1|U15187|XCLB 4-Coumarate--CoA ligase from Mycobacterium leprae (352 aa), FASTA scores: opt: 1815,E(): 0, (78.9% identity in 351 aa overlap). Also similar to N-terminus of acid-CoA ligases e.g. NP_471116.1|NC_003212 O-succinylbenzoic acid-CoA ligase from Listeria innocua (469 aa); NP_390957.1|NC_000964 O-succinylbenzoic acid-CoA ligase from Bacillus subtilis (486 aa); MENE_HAEIN|P44565 O-succinylbenzoic acid-CoA ligase from Haemophilus influenzae (452 aa), FASTA scores: opt: 307, E(): 4.6e-12,(25.4% identity in 339 aa overlap); etc. Also some similarity with fadD proteins from Mycobacterium tuberculosis. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase) 635504 menE 887507 menE Mycobacterium tuberculosis H37Rv Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase) NP_215056.1 634416 R 83332 CDS NP_215057.1 15607683 887494 complement(635573..635875) 1 NC_000962.3 Rv0543c, (MTCY25D10.22c), len: 100 aa. Conserved protein, equivalent to Q50171|MLU15187_32|NP_302469.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (100 aa),FASTA scores: opt: 493, E(): 6.1e-30, (73.5% identity in 98 aa overlap). Some similarity to Rv3046c|NP_217562.1 from Mycobacterium tuberculosis. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004). Alternative nucleotide at position 635633 (C->T; A81A) has been observed.; hypothetical protein 635875 887494 Rv0543c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215057.1 635573 R 83332 CDS NP_215058.1 15607684 887511 complement(635935..636213) 1 NC_000962.3 Rv0544c, (MTCY25D10.23c), len: 92 aa. Possible conserved transmembrane protein, equivalent to NP_302470.1|NC_002677 possible membrane protein from Mycobacterium leprae (96 aa); and shows some similarity to MLU15187_33|Q50172|U296V from Mycobacterium leprae (36 aa),FASTA scores: opt: 151, E(): 2.1e-05, (71.4% identity in 35 aa overlap). Also some similarity with VATL_NEPNO|Q26250 vacuolar ATP synthase 16 kDa proteolipid from Nephrops norvegicus (159 aa), FASTA scores: opt: 80, E(): 11, (26.1% identity in 88 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Possible conserved transmembrane protein 636213 887511 Rv0544c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_215058.1 635935 R 83332 CDS NP_215059.1 15607685 887517 complement(636210..637463) 1 NC_000962.3 Rv0545c, (MTCY25D10.24c), len: 417 aa. Probable pitA, low-affinity inorganic phosphate transporter,integral membrane protein, equivalent to Q50173|NP_302471.1 pitA from Mycobacterium leprae (414 aa), FASTA scores: opt: 2035, E(): 0, (76.3% identity in 418 aa overlap). Also highly similar to others e.g. CAB59461.1|AL132644 putative low-affinity phosphate transport protein from Streptomyces coelicolor (423 aa); PITA_ECOLI|P37308 low-affinity inorganic phosphate transporter from Escherichia coli (499 aa), FASTA scores: opt: 304, E(): 6.9e-10, (32.5 % identity in 234 aa overlap); etc. Belongs to the PHO-4 family of transporters, pit subfamily.; Probable low-affinity inorganic phosphate transporter integral membrane protein PitA 637463 pitA 887517 pitA Mycobacterium tuberculosis H37Rv Probable low-affinity inorganic phosphate transporter integral membrane protein PitA NP_215059.1 636210 R 83332 CDS NP_215060.1 15607686 887509 complement(637583..637969) 1 NC_000962.3 Rv0546c, (MTCY25D10.25c), len: 128 aa. Conserved protein, equivalent to AAA63111.1|U15187|Q50174|U296X hypothetical protein from Mycobacterium leprae (144 aa),FASTA scores: opt: 748, E(): 0, (84.2% identity in 133 aa overlap). Also highly similar to CAB95979.1|AL360034 conserved hypothetical protein from Streptomyces coelicolor (130 aa); and similar to AE000854_8|O26852 S-D-lactoylglutathione methylglyoxal lyase from Methanobacterium thermoautotropto (116 aa), FASTA scores: opt: 155, E(): 0.00019, (30.6% identity in 108 aa overlap); YAER_ECOLI hypothetical 14.7 kDa protein from Escherichia coli (129 aa), FASTA scores: opt: 104, E(): 0.42, (28.7% identity in 115 aa overlap). Also similar to Rv2068c from Mycobacterium tuberculosis.; hypothetical protein 637969 887509 Rv0546c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215060.1 637583 R 83332 CDS NP_215061.1 15607687 887527 complement(638032..638916) 1 NC_000962.3 Rv0547c, (MTCY25D10.26c), len: 294 aa. Possible oxidoreductase, similar to various oxidoreductases e.g. fatty acyl-CoA reductase from Acinetobacter calcoaceticus (295 aa); NP_280196.1|NC_002607 3-oxoacyl-[acyl-carrier-protein] reductase from Halobacterium sp. NRC-1 (255 aa); NP_349214.1|NC_003030 Short-chain alcohol dehydrogenase family protein from Clostridium acetobutylicum (255 aa); etc. Also similar to several proteins from Mycobacterium tuberculosis e.g. Y04M_MYCTU|Q10783 putative oxidoreductase (341 aa), FASTA scores: opt: 644, E(): 0, (46.1% identity in 258 aa overlap).; Possible oxidoreductase 638916 887527 Rv0547c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_215061.1 638032 R 83332 CDS NP_215062.1 15607688 887529 complement(639012..639956) 1 NC_000962.3 Rv0548c, (MTCY25D10.27c), len: 314 aa. menB,naphthoate synthase (dihydroxynaphthonic acid synthase),equivalent to NP_302473.1|NC_002677 naphthoate synthase from Mycobacterium leprae (300 aa). Also similar to others e.g. MENB_ECOLI|P27290 naphthoate synthase from Escherichia coli (285 aa), FASTA scores: opt: 599, E(): 9.3e-33, (48.1 identity in 285 aa overlap); etc. Belongs to the enoyl-CoA hydratase/isomerase family.; Naphthoate synthase MenB (dihydroxynaphthoic acid synthetase) (DHNA synthetase) 639956 menB 887529 menB Mycobacterium tuberculosis H37Rv Naphthoate synthase MenB (dihydroxynaphthoic acid synthetase) (DHNA synthetase) NP_215062.1 639012 R 83332 CDS NP_215063.1 15607689 887534 complement(640228..640641) 1 NC_000962.3 Rv0549c, (MTCY25D10.28c), len: 137 aa. Possible vapC3, toxin, part of toxin-antitoxin (TA) operon with Rv0550c, contains PIN domain (see Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others e.g. Rv0960,Rv0065, and Rv1720c from Mycobacterium tuberculosis.; Possible toxin VapC3 640641 vapC3 887534 vapC3 Mycobacterium tuberculosis H37Rv Possible toxin VapC3 NP_215063.1 640228 R 83332 CDS NP_215064.1 15607690 887515 complement(640638..640904) 1 NC_000962.3 Rv0550c, (MTCY25D10.29c), len: 88 aa. Possible vapB3, antitoxin, part of toxin-antitoxin (TA) operon with Rv0549c (See Arcus et al., 2005; Pandey and Gerdes, 2005).; Possible antitoxin VapB3 640904 vapB3 887515 vapB3 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB3 NP_215064.1 640638 R 83332 CDS NP_215065.1 15607691 887526 complement(641096..642811) 1 NC_000962.3 Rv0551c, (MTCY25D10.30c), len: 571 aa. Probable fadD8, fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 585, E(): 9.5e-30, (28.7% identity in 536 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Note other possible start sites exist downstream of this start.; Probable fatty-acid-CoA ligase FadD8 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 642811 fadD8 887526 fadD8 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD8 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_215065.1 641096 R 83332 CDS NP_215066.1 15607692 887537 642889..644493 1 NC_000962.3 Rv0552, (MTCY25D10.31), len: 534 aa. Conserved protein, similar to others from several organisms. Also shows some similarity with regulatory proteins e.g. AEPA_ERWCA|Q06555 exoenzymes regulatory protein aepA [Precursor] from Erwinia carotovora (465 aa), FASTA scores: opt: 278, E(): 7.6e-11, (23.0% identity in 408 aa overlap). Also similar to Z99119|BSUB0016_28 from Bacillus subtilis (529 aa), FASTA scores: opt: 436, E(): 8.3e-20, (23.8% identity in 547 aa overlap). C-terminus is similar to MLRRNOPR_1 hypothetical 17.7 kDa protein from Mycobacterium leprae (154 aa), FASTA score: (43.1% identity in 160 aa overlap).; hypothetical protein 644493 887537 Rv0552 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215066.1 642889 D 83332 CDS NP_215067.1 15607693 887544 644490..645470 1 NC_000962.3 Rv0553, (MTCY25D10.32), len: 326 aa. Probable menC,muconate cycloisomerase, equivalent to NP_302476.1|NC_002677 putative isomerase/racemase from Mycobacterium leprae (334 aa). Also similar to other muconate cycloisomerases e.g. TCBD_PSESP|P27099 chloromuconate cycloisomerase (370 aa), FASTA scores: opt: 249, E(): 7.8e-09, (32.7% identity in 199 aa overlap). Also similar to O-succinylbenzoate-CoA synthases. Belongs to the mandelate racemase / muconate lactonizing enzyme family.; Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE) 645470 menC 887544 menC Mycobacterium tuberculosis H37Rv Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE) NP_215067.1 644490 D 83332 CDS NP_215068.1 15607694 887535 645467..646255 1 NC_000962.3 Rv0554, (MTCY25D10.33), len: 262 aa. Possible bpoC,peroxidase (non-haem peroxidase), equivalent to NP_302477.1|NC_002677 putative hydrolase from Mycobacterium leprae (265 aa). Also highly similar or similar to various hydrolases and peroxidases e.g. CAB38877.1|AL035707|T36181 probable hydrolase from Streptomyces coelicolor (272 aa); CAC48368.1|Y16952 putative hydrolase from Amycolatopsis mediterranei (284 aa); P29715|BPA2_STRAU non-haem bromoperoxidase bpo-a2 (bromide peroxidase) from Streptomyces aureofaciens (277 aa), FASTA scores: opt: 325,E(): 2.3e-15, (29.5% identity in 268 aa overlap); O31168|PRXC_STRAU|CPO|CPOT non-heme chloroperoxidase (chloride peroxidase) from Streptomyces aureofaciens (278 aa); etc. Also similar to M. tuberculosis non-heme haloperoxidases and epoxide hydrolases e.g. Rv1938, Rv3617,etc.; Possible peroxidase BpoC (non-haem peroxidase) 646255 bpoC 887535 bpoC Mycobacterium tuberculosis H37Rv Possible peroxidase BpoC (non-haem peroxidase) NP_215068.1 645467 D 83332 CDS NP_215069.1 15607695 887554 646298..647962 1 NC_000962.3 Rv0555, (MTCY25D10.34), len: 554 aa. Probable menD,menaquinone biosynthesis protein, including 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase and 2-oxoglutarate decarboxylase activities. Equivalent to NP_302478.1|NC_002677 putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase from Mycobacterium leprae (556 aa). Also similar to others e.g. MEND_BACSU|P23970 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase from Bacillus subtilis (548 aa), FASTA scores: opt: 488, E(): 2.3e-21, (34.3% identity in 545 aa overlap); etc. Cofactor: thiamine pyrophosphate.; Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha-ketoglutarate decarboxylase) (KDC) 647962 menD 887554 menD Mycobacterium tuberculosis H37Rv Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha-ketoglutarate decarboxylase) (KDC) NP_215069.1 646298 D 83332 CDS NP_215070.1 15607696 887551 647959..648474 1 NC_000962.3 Rv0556, (MTCY25D10.35), len: 171 aa. Probable conserved transmembrane protein, equivalent to NP_302479.1|NC_002677 putative membrane protein from Mycobacterium leprae (175 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved transmembrane protein 648474 887551 Rv0556 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215070.1 647959 D 83332 CDS NP_215071.1 15607697 887609 648536..649672 1 NC_000962.3 Rv0557, (MTCY25D10.36), len: 378 aa. MgtA (previously known as pimB), mannosyltransferase (see citation below), similar to other various transferases e.g. NP_243554.1|NC_002570 alpha-D-mannose-alpha(1-6)phosphatidyl myo-inositol monomannoside transferase from Bacillus halodurans (381 aa); NP_249533.1|NC_002516 probable glycosyl transferase from Pseudomonas aeruginosa (406 aa); NP_419573.1|NC_002696 glycosyl transferase, group 1 family protein, from Caulobacter crescentus (455 aa); etc. Also similar to Q55598 hypothetical 44.9 kDa protein from synechocystis SP (409 aa), FASTA scores: opt: 703, E(): 0, (33.9% identity in 378 aa overlap); GPI3_YEAST|P32363 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (452 aa), FASTA scores: opt: 230, E(): 1.1e-07,(23.5% identity in 328 aa overlap).; Mannosyltransferase MgtA 649672 mgtA 887609 mgtA Mycobacterium tuberculosis H37Rv Mannosyltransferase MgtA NP_215071.1 648536 D 83332 CDS YP_177738.1 57116754 887591 649689..650393 1 NC_000962.3 Rv0558, (MTCY25D10.37), len: 234 aa. Probable menH (alternate gene name: menG), ubiquinone/menaquinone biosynthesis methlytransferase (2-heptaprenyl-1,4-naphthoquinone methyltransferase),equivalent to NP_302480.1|NC_002677 putative ubiquinone/menaquinone biosynthesis methyltransferase from Mycobacterium leprae (238 aa). Also highly similar to others e.g. CAB44537.1|AL078618|T34630 from Streptomyces coelicolor (231 aa); UBIE_ECOLI|P27851 from Escherichia coli strain K12 (251 aa), FASTA scores: opt: 421, E(): 1.2e-21, (43.2% identity in 227 aa overlap); GRC2_BACSU|P31113 from Bacillus subtilis (233 aa), FASTA scores: opt: 345, E(): 1.4e-16, (34.6% identity in 231 aa overlap); etc. Belongs to the UbiE family. Note that previously known as ubiE.; Probable ubiquinone/menaquinone biosynthesis methyltransferase MenH (2-heptaprenyl-1,4-naphthoquinone methyltransferase) 650393 menH 887591 menH Mycobacterium tuberculosis H37Rv Probable ubiquinone/menaquinone biosynthesis methyltransferase MenH (2-heptaprenyl-1,4-naphthoquinone methyltransferase) YP_177738.1 649689 D 83332 CDS NP_215073.1 15607699 887569 complement(650407..650745) 1 NC_000962.3 Rv0559c, (MTCY25D10.38c), len: 112 aa. Possible conserved secreted protein, similar to NP_302481.1|NC_002677 putative secreted protein from Mycobacterium leprae (112 aa). Also similar to Y08B_MYCTU|Q11048 hypothetical 11.6 kDa protein FASTA scores: opt: 111, E(): 011, (25.4% identity in 114 aa overlap). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved secreted protein 650745 887569 Rv0559c Mycobacterium tuberculosis H37Rv Possible conserved secreted protein NP_215073.1 650407 R 83332 CDS NP_215074.1 15607700 887637 complement(650779..651504) 1 NC_000962.3 Rv0560c, (MTCY25D10.39c), len: 241 aa. Possible benzoquinone methyltransferase (see citation below),similar to other hypothetical proteins and methyltransferases e.g. Q54300 methyltransferase (211 aa),FASTA scores: opt: 203, E(): 4.8e-07, (30.9% identity in 136 aa overlap). Similar to Rv3699, Rv1377c, Rv2675c, etc from Mycobacterium tuberculosis. Rv0560c can be induced by salicylate and para-amino-salicylate (pas).; Possible benzoquinone methyltransferase (methylase) 651504 887637 Rv0560c Mycobacterium tuberculosis H37Rv Possible benzoquinone methyltransferase (methylase) NP_215074.1 650779 R 83332 CDS NP_215075.1 15607701 887638 complement(651529..652755) 1 NC_000962.3 Rv0561c, (MTCY25D10.40c), len: 408 aa. Possible oxidoreductase, highly similar (except in first 30 aa) to NP_302482.1|NC_002677 putative FAD-linked oxidoreductase from Mycobacterium leprae (408 aa). Also similar to T34627 probable electron transfer oxidoreductase from Streptomyces coelicolor (430 aa); and some bacteriochlorophyll synthases e.g. NP_069300.1|NC_000917 bacteriochlorophyll synthase from Archaeoglobus fulgidus (410 aa); Q55087 geranylgeranyl hydrogenase (407 aa), FASTA scores: opt: 208, E(): 1.7e-06,(26.9% identity in 327 aa overlap).; Possible oxidoreductase 652755 887638 Rv0561c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_215075.1 651529 R 83332 CDS NP_215076.1 15607702 887647 652771..653778 1 NC_000962.3 Rv0562, (MTCY25D10.41), len: 335 aa. Probable grcC1,polyprenyl diphosphate synthetase, equivalent to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl) e.g. GRC3_BACSU|P31114 probable heptaprenyl diphosphate syntetase (348 aa), FASTA scores: opt: 599, E(): 4e-31,(33.2% identity in 307 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins Rv0989c|grcC2|NP_215504.1|MTCI237.03c probable polyprenyl-diphosphate synthase (325 aa); Rv3383c, Rv3398c,etc. Contains PS00444 Polyprenyl synthetases signature 2. Belongs to the FPP/GGPP synthetases family.; Probable polyprenyl-diphosphate synthase GrcC1 (polyprenyl pyrophosphate synthetase) 653778 grcC1 887647 grcC1 Mycobacterium tuberculosis H37Rv Probable polyprenyl-diphosphate synthase GrcC1 (polyprenyl pyrophosphate synthetase) NP_215076.1 652771 D 83332 CDS NP_215077.1 15607703 887649 653879..654739 1 NC_000962.3 Rv0563, (MTV039.01, MTCY25D10.42), len: 286 aa. (alternative start at position 654006). Probable htpX,protease heat shock protein X (transmembrane protein),equivalent to NP_302484.1|NC_002677 putative peptidase from Mycobacterium leprae (287 aa). Also highly similar to others e.g. CAC08262.1|AL392146 putative peptidase from Streptomyces coelicolor (287 aa); NP_387431.1|NC_003047 putative protease transmembrane protein from Sinorhizobium meliloti (319 aa); NP_105051.1|NC_002678 heat shock protein (htpX) from Mesorhizobium loti (336 aa); NP_248692.1|NC_000909|U67608|MJU67608_8 heat shock protein HtpX, possibly protease (htpX) from Methanococcus jannaschii (284 aa), FASTA scores: opt: 660, E(): 0, (46.5 identity in 245 aa overlap). Continuation of MTCY25D10.42. Belongs to peptidase family M48 (zinc metalloprotease). Cofactor: Zinc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable protease transmembrane protein heat shock protein HtpX 654739 htpX 887649 htpX Mycobacterium tuberculosis H37Rv Probable protease transmembrane protein heat shock protein HtpX NP_215077.1 653879 D 83332 CDS NP_215078.1 15607704 887651 complement(654924..655949) 1 NC_000962.3 Rv0564c, (MTV039.02c), len: 341 aa. Possible gpdA1(alternate gene names: gpsA, glyC),glycerol-3-phosphate dehydrogenase [NAD(P)+] dependent,similar to many other glycerol-3-phosphate dehydrogenases e.g. P46919|GPDA_BACSU from Bacillus subtilis (345 aa),FASTA scores: opt: 731, E(): 0, (37.3% identity in 332 aa overlap); etc. Also similar to Rv2982c|gpdA2|MTCY349.05|Z83018|MTCY349_5 from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 740, E(): 0, (40.4% identity in 322 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.; Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA1 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) (NAD(P)H-dependent dihydroxyacetone-phosphate reductase) 655949 gpdA1 887651 gpdA1 Mycobacterium tuberculosis H37Rv Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA1 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) (NAD(P)H-dependent dihydroxyacetone-phosphate reductase) NP_215078.1 654924 R 83332 CDS NP_215079.1 15607705 887662 complement(656010..657470) 1 NC_000962.3 Rv0565c, (MTV039.03c), len: 486 aa. Probable monoxygenase, highly similar to NP_301173.1|NC_002677 putative monooxygenase from Mycobacterium leprae (494 aa). Also highly similar to others e.g. NP_421371.1|NC_002696 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa); C-terminus of NP_051574.1|NC_000958 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa); P12015|CYMO_ACISP cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 354, E(): 2.1e-16, (23.7% identity in 435 aa overlap); etc. Also similar to other putative monoxygenases from Mycobacterium tuberculosis e.g. Rv3854c (489 aa), MTCY01A6.14 (489 aa),MTV013_4 (495 aa), MTCY31.20 (495 aa).; Probable monooxygenase 657470 887662 Rv0565c Mycobacterium tuberculosis H37Rv Probable monooxygenase NP_215079.1 656010 R 83332 CDS NP_215080.1 15607706 887633 complement(657548..658039) 1 NC_000962.3 Rv0566c, (MTV039.04c), len: 163 aa. Conserved protein, similar to others e.g. P77482|YAJQ_ECOLI hypothetical 19.0 KDa protein from Escherichia coli (169 aa), FASTA scores: opt: 422, E(): 5.4e-20, (44.1 identity in 161 aa overlap); etc.; hypothetical protein 658039 887633 Rv0566c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215080.1 657548 R 83332 CDS NP_215081.1 15607707 887667 658321..659340 1 NC_000962.3 Rv0567, (MTV039.05), len: 339 aa. Probable methyltransferase, similar to several e.g. P39896|TCMO_STRGA tetracenomycin polyketide synthesis 8-O-methyltransferase from Streptomyces glaucescens (339 aa), FASTA scores: opt: 685, E(): 0, (35.8% identity in 335 aa overlap); P10950|HIOM_BOVIN hydroxyindole O-methyltransferase from Bos taurus (345 aa), FASTA scores: opt: 509, E(): 3.4e-27, (30.7% identity in 332 aa overlap) etc.; Probable methyltransferase/methylase 659340 887667 Rv0567 Mycobacterium tuberculosis H37Rv Probable methyltransferase/methylase NP_215081.1 658321 D 83332 CDS NP_215082.1 15607708 887654 659450..660868 1 NC_000962.3 Rv0568, (MT0594, MTV039.06), len: 472 aa. Possible cyp135B1, cytochrome P450, similar to putative cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. P29980|CPXN_ANASP probable cytochrome P450 from Anabaena sp. strain PCC 7120 (459 aa), FASTA scores: opt: 525, E(): 7.2e-27, (31.9% identity in 417 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0327c|NP_214841.1|NC_000962|CYP135A1|MT0342|MTCY63.32c putative cytochrome P450 (449 aa), FASTA scores: opt: 1080,E(): 0, (40.5% identity in 444 aa overlap); Rv3685c|NP_218202.1|NC_000962 putative cytochrome P450 (476 aa); Rv0136|NP_214650.1|NC_000962 putative cytochrome P450 (441 aa); etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086).; Possible cytochrome P450 135B1 Cyp135B1 660868 cyp135B1 887654 cyp135B1 Mycobacterium tuberculosis H37Rv Possible cytochrome P450 135B1 Cyp135B1 NP_215082.1 659450 D 83332 CDS NP_215083.1 15607709 887678 661003..661269 1 NC_000962.3 Rv0569, (MTV039.07), len: 88 aa. Conserved protein. C-terminus highly similar to AAA63065.1|U15184|MLU15184_10 hypothetical protein from Mycobacterium leprae (53 aa),FASTA scores: opt: 140, E(): 0.0046, (64.7% identity in 34 aa overlap). Also similar to T36824|SCI35.11 hypothetical protein from Streptomyces coelicolor (64 aa); and N-terminus of T36956 probable DNA-binding protein from Streptomyces coelicolor (323 aa). Also highly similar to Rv2302|MTCY339.07c|NP_216818.1|NC_000962 conserved hypothetical protein from Mycobacterium tuberculosis (80 aa), FASTA scores: opt: 300, E(): 1.4e-13, (61.8% identity in 76 aa overlap).; hypothetical protein 661269 887678 Rv0569 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215083.1 661003 D 83332 CDS NP_215084.1 15607710 887666 661295..663373 1 NC_000962.3 Rv0570, (MTV039.08), len: 692 aa. Probable nrdZ,ribonucleoside-diphosphate reductase, large subunit, highly similar to others e.g. NP_070492.1|NC_000917|NRD|AE000988_11 ribonucleotide reductase from Archaeoglobus fulgidus (752 aa), FASTA scores: opt: 2001, E(): 0, (52.5% identity in 562 aa overlap) (N-terminus shorter); U73619|TAU73619_1|T37459 ribonucleotide reductase from Thermoplasma acidophilum (857 aa), FASTA scores: opt: 1678, E(): 0, (43.7% identity in 723 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase large chain family.; Probable ribonucleoside-diphosphate reductase (large subunit) NrdZ (ribonucleotide reductase) 663373 nrdZ 887666 nrdZ Mycobacterium tuberculosis H37Rv Probable ribonucleoside-diphosphate reductase (large subunit) NrdZ (ribonucleotide reductase) NP_215084.1 661295 D 83332 CDS NP_215085.1 15607711 887710 complement(663487..664818) 1 NC_000962.3 Rv0571c, (MTV039.09c), len: 443 aa. Conserved protein, highly similar to the products of two adjacent orfs in Mycobacterium leprae: AAA63059.1|U15184|U650S|Q50111 hypothetical protein (258 aa), FASTA scores: opt: 1071, E(): 0, (72.5% identity in 233 aa overlap); and AAA63058.1|U15184|U650T hypothetical protein (86 aa), FASTA scores: opt: 192, E(): 6.4e-06,(70.8% identity in 48 aa overlap). Also similar to others e.g. NP_107072.1|NC_002678 hypothetical protein from Mesorhizobium loti (235 aa); NP_213031.1|NC_000918 hypothetical protein from Aquifex aeolicus (175 aa); etc. And similar to part of hypothetical proteins from Mycobacterium tuberculosis e.g. C-terminus of Rv2143|MTCY270.25c|Z95388|NP_216659.1|NC_000962 (352 aa),FASTA scores: opt: 592, E(): 7e-32, (49.3% identity in 205 aa overlap); N-terminus of Rv2030c|NP_216546.1|NC_000962 (681 aa).; hypothetical protein 664818 887710 Rv0571c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215085.1 663487 R 83332 CDS NP_215086.1 15607712 887696 complement(665042..665383) 1 NC_000962.3 Rv0572c, (MTV039.10c), len: 113 aa. Hypothetical unknown protein.; Hypothetical protein 665383 887696 Rv0572c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215086.1 665042 R 83332 CDS NP_215087.1 15607713 887716 complement(665851..667242) 1 NC_000962.3 Rv0573c, (MTV039.11c), len: 463 aa. PncB2, nicotinic acid phosphoribosyltransferase (See Boshoff et al., 2008). Similar to e.g. NP_213718.1|NC_000918 hypothetical protein from Aquifex aeolicus (426 aa); AL109962|T36953|SCJ1.20 conserved hypothetical protein from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1089, E(): 0, (49.4% identity in 385 aa overlap); P_391053.1|Z99120|BSUB0017_57|NC_000964 protein similar to nicotinate phosphoribosyltransferase from Bacillus subtilis (490 aa), FASTA scores: opt: 955,E():0, (43.5% identity in 356 aa overlap); etc. Also similar to Q10641|Y03F_MYCTU|MTCY130.15c|Rv1330c conserved hypothetical protein from Mycobacterium tuberculosis (509 aa), FASTA scores: opt: 761, E(): 0, (38.4% identity in 437 aa overlap).; Nicotinic acid phosphoribosyltransferase PncB2 667242 pncB2 887716 pncB2 Mycobacterium tuberculosis H37Rv Nicotinic acid phosphoribosyltransferase PncB2 NP_215087.1 665851 R 83332 CDS NP_215088.1 15607714 887721 complement(667252..668394) 1 NC_000962.3 Rv0574c, (MTV039.12c), len: 380 aa. Conserved hypothetical protein, showing similarity with other hypothetical proteins and polyglutamate synthases (encapsulation proteins) e.g. AAK64444.1|AF377339_5|AF377339 polyglutamate synthase CapA from Myxococcus xanthus (405 aa); M24150|BACCAPABC_3|CapA polyglutamate synthase (encapsulation protein) from B.anthracis (411 aa), FASTA scores: opt: 261, E(): 4.3e-10,(25.8% identity in 287 aa overlap); etc.; hypothetical protein 668394 887721 Rv0574c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215088.1 667252 R 83332 CDS NP_215089.1 15607715 887720 complement(668579..669745) 1 NC_000962.3 Rv0575c, (MTV039.13c), len: 388 aa. Possible oxidoreductase, similar to many diverse oxidoreductases and monooxygenases e.g. AL109974|SCF34_5|T36404 probable monooxygenase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 786, E(): 0, (38.7% identity in 398 aa overlap); P96555|AB000564 salicylate hydroxylase from sphingomonas (395 aa), FASTA scores: opt: 267, E():5e-11,(26.4% identity in 390 aa overlap). Also similar to Rv1260|Z77137|MTCY50.22C from Mycobacterium tuberculosis (372 aa), FASTA scores: opt: 762, E(): 0, (40.9% identity in 345 aa overlap). The transcription of this CDS seems to be activated in macrophages (see citation below).; Possible oxidoreductase 669745 887720 Rv0575c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_215089.1 668579 R 83332 CDS NP_215090.1 15607716 887717 669848..671152 1 NC_000962.3 Rv0576, (MTV039.14), len: 434 aa. Probable transcriptional regulator, ArsR family. N-terminus highly similar to others e.g. NP_102487.1|NC_002678 transcriptional regulator from Mesorhizobium loti (104 aa); NP_242952.1|NC_002570 transcriptional regulator (ArsR family) from Bacillus halodurans (109 aa); etc. C-terminal region (~240-434) shows similarity with D67028_1 from Rhodococcus rhodochrous (112 aa); and Rv0738 from Mycobacterium tuberculosis (182 aa). N-terminus also highly similar to Rv2034 from Mycobacterium tuberculosis (107 aa). Contains helix-turn-helix motif at aa 23-43 (Score 1628,+4.73 SD).; Probable transcriptional regulatory protein (possibly ArsR-family) 671152 887717 Rv0576 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (possibly ArsR-family) NP_215090.1 669848 D 83332 CDS NP_215091.1 15607717 887732 671166..671951 1 NC_000962.3 Rv0577, (MTV039.15), len: 261 aa. TB27.3, conserved protein. Corresponds to O53774|CF30_MYCTU 27 kDa antigen CFP30B from Mycobacterium tuberculosis culture filtrate (260 aa), FASTA scores: opt: 1781, E(): 0, (100.0% identity in 260 aa overlap). Also similar to several hypothetical proteins and hydroxylases from Steptomyces sp. e.g. T35032 probable hydroxylase from Streptomyces coelicolor (263 aa); Q55078 orfA gene product from Streptomyces sp. (275 aa),FASTA scores: E(): 1.5e-1 9, (38.6% identity in 264 aa overlap); D89734_1|P95754 DNA for SgaA SGAA protein from Streptomyces griseus; and SC9B10_20 from Streptomyces coelicolor (267 aa), FASTA score: (38.9 identity in 252 aa overlap). Also similar to Rv0911|MTCY21C12.05 from Mycobacterium tuberculosis (257 aa), FASTA scores: E(): 1.1e-20, (32.0% identity in 259 aa overlap).; Conserved protein TB27.3 671951 TB27.3 887732 TB27.3 Mycobacterium tuberculosis H37Rv Conserved protein TB27.3 NP_215091.1 671166 D 83332 CDS YP_177739.1 57116755 887725 complement(671996..675916) 1 NC_000962.3 Rv0578c, (MTV039.16c), len: 1306 aa. PE_PGRS7,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many other PGRS proteins e.g. MTCY493.04|Z95844 from Mycobacterium tuberculosis (1329 aa), FASTA scores: opt: 3994, E(): 0, (54.6% identity in 1375 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures possibly fortuitously.; PE-PGRS family protein PE_PGRS7 675916 PE_PGRS7 887725 PE_PGRS7 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS7 YP_177739.1 671996 R 83332 CDS NP_215093.1 15607719 887738 676238..676996 1 NC_000962.3 Rv0579, (MTV039.17), len: 252 aa. Conserved hypothetical protein, showing some similarity to others e.g. AE001747_4 hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 612, E(): 0, (39.6% identity in 235 aa overlap); AE001004_2 hypothetical protein from Archaeoglobus fulgidus (159 aa), FASTA scores: opt: 196, E(): 1e-06, (28.3% identity in 159 aa overlap); etc.; hypothetical protein 676996 887738 Rv0579 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215093.1 676238 D 83332 CDS NP_215094.1 15607720 887712 complement(677125..677616) 1 NC_000962.3 Rv0580c, (MTV039.18c), len: 163 aa. Conserved protein, equivalent to AAA90989.1|U20446|MK35 lipoprotein precursor from Mycobacterium kansasii (225 aa).; hypothetical protein 677616 887712 Rv0580c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215094.1 677125 R 83332 CDS NP_215095.1 15607721 887739 677710..677925 1 NC_000962.3 Rv0581, (MTV039.19), len: 71 aa. Possible vapB26,antitoxin, part of toxin-antitoxin (TA) operon with Rv0582,see Arcus et al. 2005. Showing weak similarity to other Mycobacterium tuberculosis proteins including P95003|Z83863|Rv2550c|MTCY159_6 conserved hypothetical protein (81 aa), FASTA scores: opt: 93, E(): 3.2, (25.7% identity in 70 aa overlap); Rv2871; Rv1241; etc. Also shows weak similarity to X05648|SGSPH_1 from Streptomyces glaucescens (77 aa), FASTA scores: opt: 92, E(): 3.6,(35.4% identity in 65 aa overlap).; Possible antitoxin VapB26 677925 vapB26 887739 vapB26 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB26 NP_215095.1 677710 D 83332 CDS NP_215096.1 15607722 887747 677922..678329 1 NC_000962.3 Rv0582, (MTV039.20), len: 135 aa. Possible vapC26,toxin, part of toxin-antitoxin (TA) operon with Rv0581,contains PIN domain, see Arcus et al. 2005.; Possible toxin VapC26 Contains PIN domain 678329 vapC26 887747 vapC26 Mycobacterium tuberculosis H37Rv Possible toxin VapC26 Contains PIN domain NP_215096.1 677922 D 83332 CDS NP_215097.1 15607723 887733 complement(678389..679075) 1 NC_000962.3 Rv0583c, (MTV039.21c), len: 228 aa. Probable lpqN,conserved lipoprotein, equivalent to AAA90989.1|U20446|MK35|U20446|MKU20446_1 lipoprotein precursor from Mycobacterium kansasii (225 aa), FASTA scores: opt: 945, E(): 0, (62.7% identity in 228 aa overlap); and similar to others from Mycobacteria e.g. Rv0040c and Rv1016c from Mycobacterium tuberculosis. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LpqN 679075 lpqN 887733 lpqN Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqN NP_215097.1 678389 R 83332 CDS NP_215098.1 15607724 887752 679229..681862 1 NC_000962.3 Rv0584, (MTV039.22), len: 877 aa. Possible conserved exported protein, similar to other hypothetical proteins which are not necessarily secreted e.g. CAB61925.1|AL133278 putative secreted protein from Streptomyces coelicolor (772 aa); AAD51075.1|AF175722_1|AF175722 immunoreactive 89kD antigen PG87 from Porphyromonas gingivalis (781 aa), FASTA scores: opt: 637, E(): 2.1e-30, (29.1% identity in 794 aa overlap); etc. Contains PS00699 Nitrogenases component 1 alpha and beta subunits signature 1. Has potential N-terminal signal peptide. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved exported protein 681862 887752 Rv0584 Mycobacterium tuberculosis H37Rv Possible conserved exported protein NP_215098.1 679229 D 83332 CDS NP_215099.1 15607725 887753 complement(681885..684272) 1 NC_000962.3 Rv0585c, (MTV039.23c, MTCY19H5.37), len: 795 aa. Probable conserved integral membrane protein. C-terminus similar to CAB88984.1|AL353864 putative integral membrane protein from Streptomyces coelicolor (299 aa); and C-terminal region of CAC01311.1|AL390968 putative integral membrane protein from Streptomyces coelicolor (925 aa). Also some similarity with Rv0204 from Mycobacterium tuberculosis.; Probable conserved integral membrane protein 684272 887753 Rv0585c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215099.1 681885 R 83332 CDS NP_215100.1 15607726 887754 684410..685132 1 NC_000962.3 Rv0586, (MTCY19H5.36c), len: 240 aa. Probable mce2R,transcriptional regulator, GntR family, part of mce2 operon, similar to many e.g. P33233|LLDR_ECOLI putative L-lactate dehydrogenase operon regulatory protein from Escherichia coli (258 aa), FASTA scores: opt: 225, E(): 9.3e-08, (26.7% identity in 232 aa overlap); etc. Also similar to other M. tuberculosis transcriptional regulators GntR proteins e.g. Rv3060c, Rv0792c, etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature and probable helix-turn helix motif from aa 35-56 (Score 1531,+4.40 SD).; Probable transcriptional regulatory protein Mce2R (GntR-family) 685132 mce2R 887754 mce2R Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein Mce2R (GntR-family) NP_215100.1 684410 D 83332 CDS NP_215101.1 15607727 887755 685129..685926 1 NC_000962.3 Rv0587, (MTCY19H5.35c), len: 265 aa. YrbE2A,hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEA type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 287, E(): 6.1e-12, (21.5% identity in 256 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 311, E(): 1.8e-83, (24.2% identity in 265 aa overlap); NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); etc.; Conserved hypothetical integral membrane protein YrbE2A 685926 yrbE2A 887755 yrbE2A Mycobacterium tuberculosis H37Rv Conserved hypothetical integral membrane protein YrbE2A NP_215101.1 685129 D 83332 CDS NP_215102.1 15607728 887761 685928..686815 1 NC_000962.3 Rv0588, (MTCY19H5.34c), len: 295 aa. YrbE2B,hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 232, E(): 8.4e-08, (22.1 % identity in 267 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 234, E(): 6.3e-08, (24.2% identity in 215 aa overlap); NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); etc.; Conserved hypothetical integral membrane protein YrbE2B 686815 yrbE2B 887761 yrbE2B Mycobacterium tuberculosis H37Rv Conserved hypothetical integral membrane protein YrbE2B NP_215102.1 685928 D 83332 CDS YP_177740.1 57116756 887745 686821..688035 1 NC_000962.3 Rv0589, (MTCY19H5.33c), len: 404 aa. Mce2A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Also highly similar, but longer 21 aa, to P72013|CAA50257.1|X70901|MTCI28.08 Mcep protein from Mycobacterium tuberculosis (432 aa), FASTA scores: opt: 1324, E(): 0, (62.6% identity in 436 aa overlap). Contains a possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). Note that previously known as mce2.; Mce-family protein Mce2A 688035 mce2A 887745 mce2A Mycobacterium tuberculosis H37Rv Mce-family protein Mce2A YP_177740.1 686821 D 83332 CDS NP_215104.1 15607730 887771 688032..688859 1 NC_000962.3 Rv0590, (MTCY19H5.32c), len: 275 aa. Mce2B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); P45391|YRBD_ECOLI hypothetical 19.6 kDa protein from Escherichia coli (183 aa), FASTA scores: opt: 160, E(): 0.00099, (28.3% identity in 166 aa overlap); P45029|YRBD_HAEIN|HI1085 hypothetical protein from Haemophilus influenzae (167 aa), FASTA scores: opt: 135,E():0.035, (25.9% identity in 143 aa overlap); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al.,2008).; Mce-family protein Mce2B 688859 mce2B 887771 mce2B Mycobacterium tuberculosis H37Rv Mce-family protein Mce2B NP_215104.1 688032 D 83332 CDS YP_177627.1 57116757 3205078 688808..689062 1 NC_000962.3 Rv0590A, len: 84 aa. Probable continuation of mce2B|Rv0590. Can find no frameshift to account for this. Possible nucleotide G missing at 688793 as there are 5 in Mycobacterium bovis but only 4 in CDC1551. Strong similarity to C-terminus of other Mce proteins e.g. AL583926|AL583926_38 from Mycobacterium leprae strain tn (346 aa), FASTA scores: E(): 1.2e-20, (67.85% identity in 84 aa overlap).; Mce-family related protein 689062 3205078 Rv0590A Mycobacterium tuberculosis H37Rv Mce-family related protein YP_177627.1 688808 D 83332 CDS NP_215105.1 15607731 887770 689059..690504 1 NC_000962.3 Rv0591, (MTCY19H5.31c), len: 481 aa. Mce2C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and may contain N-terminal signal or anchor sequence. Has highly Pro-rich C-terminus.; Mce-family protein Mce2C 690504 mce2C 887770 mce2C Mycobacterium tuberculosis H37Rv Mce-family protein Mce2C NP_215105.1 689059 D 83332 CDS NP_215106.1 15607732 887786 690501..692027 1 NC_000962.3 Rv0592, (MTCY19H5.30c), len: 508 aa. Mce2D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Has highly Pro-rich C-terminus and may contain N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce2D 692027 mce2D 887786 mce2D Mycobacterium tuberculosis H37Rv Mce-family protein Mce2D NP_215106.1 690501 D 83332 CDS NP_215107.1 15607733 887829 692024..693232 1 NC_000962.3 Rv0593, (MTCY19H5.29c), len: 402 aa. Possible lprL (alternate gene name: mce2E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Possible Mce-family lipoprotein LprL (Mce-family lipoprotein Mce2E) 693232 lprL 887829 lprL Mycobacterium tuberculosis H37Rv Possible Mce-family lipoprotein LprL (Mce-family lipoprotein Mce2E) NP_215107.1 692024 D 83332 CDS NP_215108.1 15607734 887797 693237..694787 1 NC_000962.3 Rv0594, (MTCY19H5.28c), len: 516 aa. Mce2F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce2F 694787 mce2F 887797 mce2F Mycobacterium tuberculosis H37Rv Mce-family protein Mce2F NP_215108.1 693237 D 83332 CDS NP_215109.1 15607735 887835 complement(694839..695231) 1 NC_000962.3 Rv0595c, (MTCY19H5.27), len: 130 aa. Possible vapC4,toxin, part of toxin-antitoxin (TA) operon with Rv0596c,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other conserved hypothetical proteins e.g. Rv0627 (135 aa) and Rv0665 (112 aa) from Mycobacterium tuberculosis; and STBB_PSESM|Q52562 plasmid stability protein from Pseudomonas syringae (139 aa), FASTA scores: opt: 131, E(): 0.0035, (35.2% identity in 88 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC4 695231 vapC4 887835 vapC4 Mycobacterium tuberculosis H37Rv Possible toxin VapC4 NP_215109.1 694839 R 83332 CDS NP_215110.1 15607736 887846 complement(695228..695485) 1 NC_000962.3 Rv0596c, (MTCY19H5.26), len: 85 aa. Possible vapB4,antitoxin, part of toxin-antitoxin (TA) operon with Rv0595c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Highly similar in part to other M. tuberculosis hypothetical proteins e.g. Rv0626, Rv3181c, Rv3385c, Rv3407, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible antitoxin VapB4 695485 vapB4 887846 vapB4 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB4 NP_215110.1 695228 R 83332 CDS NP_215111.1 15607737 887853 complement(695668..696903) 1 NC_000962.3 Rv0597c, (MTCY19H5.25), len: 411 aa. Conserved hypothetical protein, highly similar to Rv3179 conserved hypothetical protein from Mycobacterium tuberculosis (429 aa). Also similar to AAF76191.1|AF271296_1|AF271296 putative ATP/GTP binding protein from Mycobacterium smegmatis (428 aa); Rv2008c|YW09_MYCTU|Q10849 conserved hypothetical protein from Mycobacterium tuberculosis (441 aa), FASTA scores: opt: 270, E(): 3.6e-11, (30.5% identity in 416 aa overlap) (N-terminus longer). Also similar to other hypothetical proteins e.g. NP_085874.1|NC_002679 hypothetical protein from Mesorhizobium loti (435 aa) (N-terminus longer). Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 696903 887853 Rv0597c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215111.1 695668 R 83332 CDS NP_215112.1 15607738 887861 complement(697154..697567) 1 NC_000962.3 Rv0598c, (MTCY19H5.24), len: 137 aa. Possible vapC27, toxin, part of toxin-antitoxin (TA) operon with Rv00599c, contains PIN domain, see Arcus et al. 2005. Similar to others e.g. Rv2596|Y0B5_MYCTU|Q50625 conserved hypothetical protein from Mycobacterium tuberculosis (134 aa), FASTA scores: opt: 254, E(): 8.2e-12, (41.5% identity in 130 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible toxin VapC27 Contains PIN domain 697567 vapC27 887861 vapC27 Mycobacterium tuberculosis H37Rv Possible toxin VapC27 Contains PIN domain NP_215112.1 697154 R 83332 CDS NP_215113.1 15607739 887856 complement(697564..697800) 1 NC_000962.3 Rv0599c, (MTCY19H5.23), len: 78 aa. Possible vapB27,antitoxin, part of toxin-antitoxin (TA) operon with Rv0598c, see Arcus et al. 2005. Similar to others e.g. Rv2595|Y0B6_MYCTU|Q50626 conserved hypothetical protein from Mycobacterium tuberculosis (81 aa), FASTA scores: opt: 160, E(): 6.2e-07, (35.8% identity in 81 aa overlap). N-terminus shows stong similarity with N-terminus of NP_104908.1|NC_002678 hypothetical protein from Mesorhizobium loti (89 aa). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible antitoxin VapB27 697800 vapB27 887856 vapB27 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB27 NP_215113.1 697564 R 83332 CDS NP_215114.2 57116758 887847 complement(697904..698410) 1 NC_000962.3 Rv0600c, (MTCY19H5.22), len: 168 aa (probable partial CDS). Two-component sensor kinase (second part),similar to part (C-termini) of many others e.g. Q04943|AFQ2_STRCO sensor protein afsq2 from Streptomyces coelicolor (535 aa), FASTA scores: opt: 347, E(): 1.9e-12,(33.0% identity in 206 aa overlap); etc. Note that sequence was checked and no errors were detected, which would allow this and the upstream ORF to be joined. Start changed since first submission (- 39 aa). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Two component sensor kinase [second part] 698410 887847 Rv0600c Mycobacterium tuberculosis H37Rv Two component sensor kinase [second part] NP_215114.2 697904 R 83332 CDS NP_215115.1 15607741 887868 complement(698524..698994) 1 NC_000962.3 Rv0601c, (MTCY19H5.21), len: 156 aa (probable partial CDS). Two-component sensor kinase (first part),similar to part (N-termini) of others e.g. Q0375|CUTS_STRLI cuts protein from streptomyces lividans (414 aa), FASTA scores: opt: 230, E(): 3.1e-08, (39.1% identity in 115 aa overlap). Note that the sequence was checked and no errors were detected that would allow this and the downstream ORF to be joined. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Two component sensor kinase [first part] 698994 887868 Rv0601c Mycobacterium tuberculosis H37Rv Two component sensor kinase [first part] NP_215115.1 698524 R 83332 CDS NP_215116.1 15607742 887870 complement(699038..699799) 1 NC_000962.3 Rv0602c, (MTCY19H5.20), len: 253 aa. tcrA,two-component DNA-binding response regulator, highly similar to others e.g. NP_107959.1|NC_002678 two-component response regulator from Mesorhizobium loti (239 aa); etc. Also similar to many other Mycobacterium tuberculosis two-component regulators e.g. Q50806|MTCY10G2.16|Rv1033c response regulator homolog TRCR (TCRV) (257 aa), FASTA score: (47.4 identity in 232 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Two component DNA binding transcriptional regulatory protein TcrA 699799 tcrA 887870 tcrA Mycobacterium tuberculosis H37Rv Two component DNA binding transcriptional regulatory protein TcrA NP_215116.1 699038 R 83332 CDS NP_215117.1 15607743 887863 699856..700167 1 NC_000962.3 Rv0603, (MTCY19H5.19c), len: 103 aa. Possible exported protein with hydrophobic stretch at aa 7-29. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible exported protein 700167 887863 Rv0603 Mycobacterium tuberculosis H37Rv Possible exported protein NP_215117.1 699856 D 83332 CDS NP_215118.1 15607744 887879 700239..701189 1 NC_000962.3 Rv0604, (MTCY19H5.18c), len: 316 aa. Probable lpqO,conserved lipoprotein, highly similar to Rv2999|lppY putative lipoprotein from Mycobacterium tuberculosis (321 aa), FASTA scores: opt: 1153, E(): 0, (53.2% identity in 312 aa overlap). Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable conserved lipoprotein LpqO 701189 lpqO 887879 lpqO Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqO NP_215118.1 700239 D 83332 CDS NP_215119.1 15607745 887872 701406..702014 1 NC_000962.3 Rv0605, (MTCY19H5.17c), len: 202 aa. Possible resolvase for IS_Y349 element, similar to several Mycobacterial hypothetical proteins and weakly similar to Q52563 resolvase from Pseudomonas syringae (210 aa), FASTA scores: opt: 99, E(): 3.1, (35.7% identity in 98 aa overlap). Contains PS00397 Site-specific recombinases active site and probable helix-turn helix motif from aa 9-30 (Score 1815, +5.37 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible resolvase 702014 887872 Rv0605 Mycobacterium tuberculosis H37Rv Possible resolvase NP_215119.1 701406 D 83332 CDS NP_215120.1 15607746 887889 702016..702759 1 NC_000962.3 Rv0606, (MTCY19H5.16c), len: 247 aa. Possible truncated transposase for IS_1536 element, highly similar to N-terminus of other transposases from Mycobacterium tuberculosis e.g. YX16_MYCTU|Q10809|Rv2885c|MT2953|MTCY274.16c putative transposase from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 1368, E(): 0, (83.5% identity in 237 aa overlap); Rv2978c, Rv0922, Rv3827c, etc. Also similar to N-terminus of MTV002_57|Rv2792 resolvase from M. tuberculosis (193 aa), FASTA score: (87.4% identity in 238 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible transposase (fragment) 702759 887889 Rv0606 Mycobacterium tuberculosis H37Rv Possible transposase (fragment) NP_215120.1 702016 D 83332 CDS NP_215121.1 15607747 887883 702813..703199 1 NC_000962.3 Rv0607, (MTCY19H5.15c), len: 128 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 703199 887883 Rv0607 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215121.1 702813 D 83332 CDS NP_215122.1 15607748 887895 703244..703489 1 NC_000962.3 Rv0608, (MTCY19H5.14c), len: 81 aa. Possible vapB28,antitoxin, part of toxin-antitoxin (TA) operon with Rv0609,see Arcus et al. 2005. Similar to several others e.g. Rv0623|P96913|MTCY20H10.04 (84 aa), FASTA scores: opt: 159,E(): 1.2e-09, (43.0% identity in 86 aa overlap); Rv2760c (89 aa); Rv1740 (70 aa), etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible antitoxin VapB28 703489 vapB28 887895 vapB28 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB28 NP_215122.1 703244 D 83332 CDS NP_215123.1 15607749 887896 703486..703887 1 NC_000962.3 Rv0609, (MTCY19H5.13c), len: 133 aa. Possible vapC28, toxin, part of toxin-antitoxin (TA) operon with Rv0608, contains PIN domain, see Arcus et al. 2005. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. YW37_MYCTU|Q10874|Rv1982c|MT2034|MTCY39.37 conserved hypothetical protein (139 aa), FASTA scores: opt: 262, E(): 8.1e-12, (39.1% identity in 128 aa overlap); MTCY20H10.05|Rv0624|MT0652|MTCY20H10.05 conserved hypothetical protein (131 aa), FASTA score: (42.9% identity in 126 aa overlap), Rv0565c, Rv3854c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC28 Contains PIN domain 703887 vapC28 887896 vapC28 Mycobacterium tuberculosis H37Rv Possible toxin VapC28 Contains PIN domain NP_215123.1 703486 D 83332 CDS YP_177628.1 57116759 3205046 703830..704057 1 NC_000962.3 Rv0609A, len: 75 aa. Conserved hypothetical protein,highly similar to part of upstream ORF Rv0612|MTCY19H5.09c conserved hypothetical protein from Mycobacterium tuberculosis (201 aa), FASTA scores: opt: 154, E(): 1.8e-05, (74.3% identity in 35 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 704057 3205046 Rv0609A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177628.1 703830 D 83332 CDS NP_215124.1 15607750 887890 complement(704752..705909) 1 NC_000962.3 Rv0610c, (MTCY19H5.11), len: 385 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 705909 887890 Rv0610c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215124.1 704752 R 83332 CDS NP_215125.1 15607751 887906 complement(705961..706344) 1 NC_000962.3 Rv0611c, (MTCY19H5.10), len: 127 aa. Hypothetical unknown protein. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 706344 887906 Rv0611c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215125.1 705961 R 83332 CDS NP_215126.1 15607752 887908 706324..706929 1 NC_000962.3 Rv0612, (MTCY19H5.09c), len: 201 aa. Conserved hypothetical protein, highly similar, but in part, to downstream ORF Rv0609A conserved hypothetical protein from Mycobacterium tuberculosis (75 aa); and showing weak similarity with other hypothetical proteins from Mycobacterium tuberculosis. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 706929 887908 Rv0612 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215126.1 706324 D 83332 CDS NP_215127.1 15607753 887913 complement(706948..709515) 1 NC_000962.3 Rv0613c, (MTCY19H5.08), len: 855 aa. Unknown protein. Contains a very short region with strong similarity to several preprotein translocases e.g. P47847|SECA_LISMO preprotein translocase seca subunit (836 aa), FASTA scores: opt: 138, E(): 0.18, (38.6% identity in 70 aa overlap, and 72.7% identity in 22 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 709515 887913 Rv0613c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215127.1 706948 R 83332 CDS NP_215128.1 15607754 887914 709356..710348 1 NC_000962.3 Rv0614, (MTCY19H5.07c), len: 330 aa. Conserved hypothetical protein, similar in part to Mycobacterium tuberculosis hypothetical proteins e.g. YY16_MYCTU|Q10685|Rv2077c|MT2137|MTCY49.16c conserved hypothetical protein (323 aa), FASTA scores: opt: 200, E(): 0.00016, (28.3% identity in 269 aa overlap); MTCY9F9_15 FASTA score: (40.3% identity in 144 aa overlap), Rv1949c,Rv2542, etc. Several start sites are possible; first start has been chosen. Note that this ORF overlaps with the upstream ORF. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 710348 887914 Rv0614 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215128.1 709356 D 83332 CDS NP_215129.1 15607755 887920 710345..710587 1 NC_000962.3 Rv0615, (MTCY19H5.06c), len: 80 aa. Probable integral membrane protein.; Probable integral membrane protein 710587 887920 Rv0615 Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_215129.1 710345 D 83332 CDS NP_215130.1 15607756 887921 complement(710584..710850) 1 NC_000962.3 Rv0616c, (MTCY19H5.05), len: 88 aa. Hypothetical unknown protein.; Hypothetical protein 710850 887921 Rv0616c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215130.1 710584 R 83332 CDS YP_007409270.1 448815397 14515850 710782..711009 1 NC_000962.3 Rv0616A, len: 75 aa. Possible vapB29, antitoxin,part of toxin-antitoxin (TA) operon with Rv0617, see Arcus et al. 2005. Similar to many others in M. tuberculosis e.g. Rv2530A (74 aa) 35.9% identity in 78 aa overlap; Possible antitoxin VapB29 711009 vapB29 14515850 vapB29 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB29 YP_007409270.1 710782 D 83332 CDS NP_215131.1 15607757 887926 711006..711407 1 NC_000962.3 Rv0617, (MTCY19H5.04c), len: 133 aa. Possible vapC29, toxin, part of toxin-antitoxin (TA) operon with Rv0616A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2494, Rv3320c, Rv0749, Rv0277c, Rv2530c, etc.; Possible toxin VapC29 Contains PIN domain 711407 vapC29 887926 vapC29 Mycobacterium tuberculosis H37Rv Possible toxin VapC29 Contains PIN domain NP_215131.1 711006 D 83332 CDS YP_177741.1 57116760 887932 711536..712231 1 NC_000962.3 Rv0618, (MTCY19H5.03c), len: 231 aa (probable partial CDS). Probable galTa, first part of galactose-1-phosphate uridylyltransferase, highly similar to N-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 296, E(): 1.4e-11, (50.8% identity in 177 aa overlap); etc. Also highly similar to N-terminal half of some UDP glucose--hexose-1-phosphate uridylyltransferases. N-terminal 28 aa similar to MTCY20H11.08|Rv0627|MTCY20H11.08 conserved hypothetical protein from Mycobacterium tuberculosis (135 aa), FASTA score: (71.4% identity in 28 overlap). Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFs to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as galT'.; Probable galactose-1-phosphate uridylyltransferase GalTa [first part] 712231 galTa 887932 galTa Mycobacterium tuberculosis H37Rv Probable galactose-1-phosphate uridylyltransferase GalTa [first part] YP_177741.1 711536 D 83332 CDS YP_177742.1 57116761 887943 <712174..712719 1 NC_000962.3 Rv0619, (MTCY19H5.02c), len: 181 aa (probable partial CDS). Probable galTb, second part of galactose-1-phosphate uridylyltransferase, highly similar to C-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 416, E(): 5.2e-22, (43.0% identity in 186 aa overlap), etc. Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFS to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as 'galT.; Probable galactose-1-phosphate uridylyltransferase GalTb [second part] 712719 galTb 887943 galTb Mycobacterium tuberculosis H37Rv Probable galactose-1-phosphate uridylyltransferase GalTb [second part] YP_177742.1 <712174 D 83332 CDS NP_215134.1 15607760 887936 712716..713807 1 NC_000962.3 Rv0620, (MTCY19H5.01c, MTCY20H10.01), len: 363 aa. Probable galK, galactokinase, similar to others e.g. P13227|GAL1_STRLI galactokinase from Streptomyces lividans (397 aa); P06976|GAL1_ECOLI galactokinase from Escherichia coli (381 aa), FASTA scores: opt: 669, E(): 0, (35.9% identity in 365 aa overlap); etc. Contains PS00106 Galactokinase signature and PS00560 Serine carboxypeptidases, histidine active site. Belongs to the GHMP kinase family. GALK subfamily.; Probable galactokinase GalK (galactose kinase) 713807 galK 887936 galK Mycobacterium tuberculosis H37Rv Probable galactokinase GalK (galactose kinase) NP_215134.1 712716 D 83332 CDS NP_215135.1 15607761 887901 714202..715266 1 NC_000962.3 Rv0621, (MTCY20H10.02), len: 354 aa. Possible membrane protein; contains potential membrane spanning regions. Also contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible membrane protein 715266 887901 Rv0621 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215135.1 714202 D 83332 CDS NP_215136.2 57116762 887942 715370..716317 1 NC_000962.3 Rv0622, (MTCY20H10.03), len: 315 aa. Possible membrane protein; contains potential membrane spanning region. Shows weak similarity with Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, etc. Start changed since first submission (-26 aa).; Possible membrane protein 716317 887942 Rv0622 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215136.2 715370 D 83332 CDS NP_215137.1 15607763 887970 716410..716664 1 NC_000962.3 Rv0623, (MTCY20H10.04), len: 84 aa. Possible vapB30,antitoxin, part of toxin-antitoxin (TA) operon with Rv0624,see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis e.g MTCY28_2|Rv1740|MTCY28.02|MTCY04C12.25 conserved hypothetical protein (70 aa), FASTA score: (73.5% identity in 68 aa overlap); MTCY4C12_25|Rv0608|MTCY19H5.14c conserved hypothetical protein (81 aa), FASTA score: (73.5 identity in 68 aa overlap); etc.; Possible antitoxin VapB30 716664 vapB30 887970 vapB30 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB30 NP_215137.1 716410 D 83332 CDS NP_215138.1 15607764 887951 716664..717059 1 NC_000962.3 Rv0624, (MTCY20H10.05), len: 131 aa. Possible vapC30, toxin, part of toxin-antitoxin (TA) operon with Rv0623, contains PIN domain, see Arcus et al. 2005. Highly similar to others in Mycobacterium tuberculosis e.g. Rv1741, Rv0609, Rv2759c,Rv0565c, Rv3854c, Rv3083, etc.; Possible toxin VapC30 Contains PIN domain 717059 vapC30 887951 vapC30 Mycobacterium tuberculosis H37Rv Possible toxin VapC30 Contains PIN domain NP_215138.1 716664 D 83332 CDS NP_215139.1 15607765 887967 complement(717153..717893) 1 NC_000962.3 Rv0625c, (MTCY20H10.06c), len: 246 aa. Probable conserved transmembrane protein, showing similarity with others e.g. CAB61866.1|AL133252 putative integral membrane protein from Streptomyces coelicolor (249 aa). Also similar to Rv1491c|MTCY277_13 from Mycobacterium tuberculosis. Contains potential membrane spanning regions.; Probable conserved transmembrane protein 717893 887967 Rv0625c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215139.1 717153 R 83332 CDS NP_215140.1 15607766 887996 718025..718285 1 NC_000962.3 Rv0626, (MTCY20H10.07), len: 86 aa. Possible vapB5,antitoxin, part of toxin-antitoxin (TA) operon with Rv0627 (See Arcus et al., 2005; Pandey and Gerdes, 2005)., similar to others in Mycobacterium tuberculosis hypothetical proteins e.g. Rv0596c, Rv3385c, Rv3407,Rv3181c, etc. Cofactor: Mg2+; Possible antitoxin VapB5 718285 vapB5 887996 vapB5 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB5 NP_215140.1 718025 D 83332 CDS NP_215141.1 15607767 887991 718282..718689 1 NC_000962.3 Rv0627, (MTCY20H11.08), len: 135 aa. Possible vapC5,toxin, part of toxin-antitoxin (TA) operon with Rv0626,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0595c and Rv0665.; Possible toxin VapC5 718689 vapC5 887991 vapC5 Mycobacterium tuberculosis H37Rv Possible toxin VapC5 NP_215141.1 718282 D 83332 CDS NP_215142.1 15607768 887986 complement(718761..719912) 1 NC_000962.3 Rv0628c, (MTCY20H10.09c), len: 383 aa. Conserved hypothetical protein, highly similar to Rv0874c|YZ02_MYCTU|Q10536 conserved hypothetical protein from Mycobacterium tuberculosis (386 aa), FASTA scores: opt: 2082, E(): 0, (81.5% identity in 383 aa overlap). Also some similarity to P72543|SPU62616_1 hypothetical protein from Synechococcus, FASTA scores: E(): 2.8e-28, (36.6 identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 719912 887986 Rv0628c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215142.1 718761 R 83332 CDS NP_215143.1 15607769 887999 complement(720005..721732) 1 NC_000962.3 Rv0629c, (MTCY20H10.10c), len: 575 aa. Probable recD, exonuclease V, alpha chain (exodeoxyribonuclease V,alpha chain) (see citation below), highly similar to other exonucleases e.g. AF157643_3|AAD46809.1|recD Escherichia coli RecD protein homolog from Mycobacterium smegmatis (554 aa); P04993|EX5A_ECOLI|B2819 exodeoxyribonuclease V 67kd polypeptide (exonuclease V alpha chain) from Escherichia coli strain K12 (608 aa), FASTA scores: opt: 512, E(): 1.9e-24, (36.9% identity in 582 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Consists of three subunits; RECB|Rv0630c, RECC|Rv0631c and RECD.; Probable exonuclease V (alpha chain) RecD (exodeoxyribonuclease V alpha chain) (exodeoxyribonuclease V polypeptide) 721732 recD 887999 recD Mycobacterium tuberculosis H37Rv Probable exonuclease V (alpha chain) RecD (exodeoxyribonuclease V alpha chain) (exodeoxyribonuclease V polypeptide) NP_215143.1 720005 R 83332 CDS NP_215144.1 15607770 888004 complement(721729..725013) 1 NC_000962.3 Rv0630c, (MTCY20H10.11c), len: 1094 aa. Probable recB, exonuclease V, beta chain (exodeoxyribonuclease V,beta chain) (see citation below), highly similar to other exonucleases e.g. AF157643_2|recB|AAD46808.1 Escherichia coli RecB protein homolog from Mycobacterium smegmatis (1083 aa); P08394|EX5B_ECOLI|RORA|B2820 exodeoxyribonuclease V 135 kDa polypeptide (exonuclease V beta chain) from Escherichia coli strain K12 (1180 aa),FASTA scores: opt: 289, E(): 4.3e-11, (29.5 identity in 1059 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the helicase family, UVRD subfamily. Consists of three subunits; RECB, RECC|Rv0631c and recd|Rv0629c.; Probable exonuclease V (beta chain) RecB (exodeoxyribonuclease V beta chain)(exodeoxyribonuclease V polypeptide) (chi-specific endonuclease) 725013 recB 888004 recB Mycobacterium tuberculosis H37Rv Probable exonuclease V (beta chain) RecB (exodeoxyribonuclease V beta chain)(exodeoxyribonuclease V polypeptide) (chi-specific endonuclease) NP_215144.1 721729 R 83332 CDS NP_215145.1 15607771 888008 complement(725013..728306) 1 NC_000962.3 Rv0631c, (MTCY20H10.12c), len: 1097 aa. Probable recC, exonuclease V, gamma chain (exodeoxyribonuclease V,gamma chain) (see Mizrahi & Andersen 1998), highly similar to other exonucleases e.g. AF157643_1|RecC|AAD46807.1 Escherichia coli RecC protein homolog from Mycobacterium smegmatis (1085 aa); P07648|EX5C_ECOLI|B2822 exodeoxyribonuclease V 125 kDa polypeptide (exonuclease V gamma chain) from Escherichia coli strain K12 (1122 aa),FASTA scores: opt: 954, E(): 0, (29.2% identity in 1109 aa overlap); etc. Consists of three subunits; RECB|Rv0630c,RECC and recd|Rv0629c. The transcription of this CDS seems to be activated specifically in host granulomas (see Ramakrishnan et al., 2000).; Probable exonuclease V (gamma chain) RecC (exodeoxyribonuclease V gamma chain)(exodeoxyribonuclease V polypeptide) 728306 recC 888008 recC Mycobacterium tuberculosis H37Rv Probable exonuclease V (gamma chain) RecC (exodeoxyribonuclease V gamma chain)(exodeoxyribonuclease V polypeptide) NP_215145.1 725013 R 83332 CDS NP_215146.1 15607772 888015 complement(728583..729278) 1 NC_000962.3 Rv0632c, (MTCY20H10.13c), len: 231 aa. Probable echA3, enoyl-CoA hydratase, almost identical to the MTU88877_1 enoyl-CoA hydratase of Mycobacterium tuberculosis field isolate NTI64719, FASTA score: (92.4% identity in 184 aa overlap). Also similar to others e.g. P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 206, E(): 6.3e-07, (31.5% identity in 232 aa overlap); etc.; Probable enoyl-CoA hydratase EchA3 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 729278 echA3 888015 echA3 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA3 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215146.1 728583 R 83332 CDS NP_215147.1 15607773 888001 complement(729327..730166) 1 NC_000962.3 Rv0633c, (MTCY20H11.14c), len: 279 aa. Possible exported protein; has hydrophobic stretch at aa 23-41.; Possible exported protein 730166 888001 Rv0633c Mycobacterium tuberculosis H37Rv Possible exported protein NP_215147.1 729327 R 83332 CDS NP_215148.1 15607774 888016 complement(730320..731033) 1 NC_000962.3 Rv0634c, (MTCY20H10.15c), len: 237 aa. Possible glyoxalase II, equivalent to NP_302290.1|NC_002677 putative glyoxylase II from Mycobacterium leprae (238 aa); and similar to U00011_3|Y0BK_MYCLE|Q49649 hypothetical 23.9 kDa protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 281, E(): 3.9e-12, (31.8% identity in 201 aa overlap). Also similar to other glyoxalases and metallo-beta-lactamase family proteins e.g. NP_386770.1|NC_003047 putative hydroxyacylglutathione hydrolase from Sinorhizobium meliloti (256 aa); etc. Also similar to other putative glyoxylases from Mycobacterium tuberculosis e.g. Rv1637c. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions.; Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) 731033 888016 Rv0634c Mycobacterium tuberculosis H37Rv Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) NP_215148.1 730320 R 83332 CDS YP_177629.1 57116763 3205041 731113..731364 1 NC_000962.3 Rv0634A, len: 83 aa. Unknown protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 731364 3205041 Rv0634A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177629.1 731113 D 83332 CDS YP_177630.1 57116764 3205042 731712..731879 1 NC_000962.3 Rv0634B, len: 55 aa. rpmG2, 50S ribosomal protein L33. Note that Mycobacterium tuberculosis has a second rpmG gene: P96925|R33H_MYCTU|Rv2057c|MTCY63A.03|rpmG1 putative 50S ribosomal protein L33 (55 aa), FASTA scores: opt: 391,E(): 2.9e-25, (100.0% identity in 55 aa overlap). Belongs to the L33P family of ribosomal proteins.; 50S ribosomal protein L33 RpmG2 731879 rpmG2 3205042 rpmG2 Mycobacterium tuberculosis H37Rv 50S ribosomal protein L33 RpmG2 YP_177630.1 731712 D 83332 CDS NP_215149.1 15607775 888032 731930..732406 1 NC_000962.3 Rv0635, (MTCY20H10.16), len: 158 aa. HadA,(3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302287.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (159 aa); and highly similar to YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 387, E(): 5.9e-21, (43.4% identity in 145 aa overlap). Also similar CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). And highly similar to two hypothetical proteins from Mycobacterium tuberculosis: Rv0504c|YV31_MYCTU|Q11168 (166 aa), FASTA scores: opt: 405,E(): 3.2e-22, (45.0% identity in 140 aa overlap); and Rv0637|MTY20H10_19 (2 ORFs downstream) (166 aa), FASTA score: (48.7% identity in 150 aa overlap).; (3R)-hydroxyacyl-ACP dehydratase subunit HadA 732406 hadA 888032 hadA Mycobacterium tuberculosis H37Rv (3R)-hydroxyacyl-ACP dehydratase subunit HadA NP_215149.1 731930 D 83332 CDS NP_215150.1 15607776 888031 732393..732821 1 NC_000962.3 Rv0636, (MTCY20H10.17), len: 142 aa. HadB,(3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302286.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (142 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also highly similar to CAB77411.1|AL160431|SCD82.08 hypothetical protein from Streptomyces coelicolor (142 aa); and similar to others e.g. U28943|CELE04F6_3 from Caenorhabditis elegans (cosmid E04) (298 aa), FASTA scores: opt: 167, E(): 0.00064, (31.6 identity in 117 aa overlap).; (3R)-hydroxyacyl-ACP dehydratase subunit HadB 732821 hadB 888031 hadB Mycobacterium tuberculosis H37Rv (3R)-hydroxyacyl-ACP dehydratase subunit HadB NP_215150.1 732393 D 83332 CDS NP_215151.1 15607777 888019 732825..733325 1 NC_000962.3 Rv0637, (MTCY20H10.18), len: 166 aa. HadC,(3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302285.1|NC_002677|YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa),FASTA scores: opt: 352, E(): 4e-19, (39.2% identity in 148 aa overlap); and highly similar to others from Mycobacterium leprae e.g. NP_302287.1|NC_002677 conserved hypothetical protein (159 aa). Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa); Rv0635|NP_215149.1|NC_000962|MTY20H10_17 conserved hypothetical protein (two ORFs upstream) from Mycobacterium tuberculosis (158 aa), FASTA score: (49.3% identity in 150 aa overlap); and Rv0504c|NP_215018.1|NC_000962|YV31_MYCTU|Q11168 hypothetical protein from Mycobacterium tuberculosis (166 aa), FASTA scores: opt: 380, E(): 3.8e-21, (43.1% identity in 137 aa overlap).; (3R)-hydroxyacyl-ACP dehydratase subunit HadC 733325 hadC 888019 hadC Mycobacterium tuberculosis H37Rv (3R)-hydroxyacyl-ACP dehydratase subunit HadC NP_215151.1 732825 D 83332 CDS YP_177743.1 57116765 888042 733737..734222 1 NC_000962.3 Rv0638, (MTCY20H10.19), len: 161 aa. Probable secE1,preprotein translocase (tail-anchored membrane protein) (see citation below), highly similar at C-terminal half to others e.g. P36690|SECE_STRGR preprotein translocase SECE subunit from Streptomyces griseus (86 aa), FASTA scores: opt: 220, E(): 4.6e-06, (35.4% identity in 96 aa overlap); P16920|SECE_ECOLI preprotein translocase sece subunit from Escherichia coli strains K12 and O157:H7 (127 aa), FASTA scores: opt: 122, E(): 0.34, (37.0% identity in 54 aa overlap); etc. Contains PS01067 Protein secE/sec61-gamma signature. Belongs to the SECE/SEC61-gamma family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE, SECF|Rv2586c,SECG|Rv1440 and SECY|Rv0732. Note that previously known as secE.; Probable preprotein translocase SecE1 734222 secE1 888042 secE1 Mycobacterium tuberculosis H37Rv Probable preprotein translocase SecE1 YP_177743.1 733737 D 83332 CDS NP_215153.1 15607779 888039 734254..734970 1 NC_000962.3 Rv0639, (MTCY20H10.20), len: 238 aa. Probable nusG,transcription antitermination protein, equivalent to NP_302283.1|NC_002677 transcription antitermination protein nusG from Mycobacterium leprae (228 aa). Also highly similar to others e.g. P36260|NUSG_STRGR from Streptomyces griseus (294 aa), FASTA scores: opt: 845, E(): 0, (55.4% identity in 233 aa overlap); etc. Note that shorter at the N-terminus than other nusG. Contains PS01014 Transcription termination factor nusG signature. Belongs to the NusG family.; Probable transcription antitermination protein NusG 734970 nusG 888039 nusG Mycobacterium tuberculosis H37Rv Probable transcription antitermination protein NusG NP_215153.1 734254 D 83332 CDS NP_215154.1 15607780 888045 735022..735450 1 NC_000962.3 Rv0640, (MTCY20H11.21), len: 142 aa. rplK, 50S ribosomal protein L11, equivalent to NP_302282.1|NC_002677 50S ribosomal protein L11 from Mycobacterium leprae (142 aa). Also highly similar to others e.g. P48954|RL11_STRCO|SCD82.19 50s ribosomal protein L11 from Streptomyces coelicolor (144 aa), FASTA scores: opt: 763,E(): 0, (84.6% identity in 143 aa overlap); etc. Contains PS00359 Ribosomal protein L11 signature. Belongs to the L11P family of ribosomal proteins.; 50S ribosomal protein L11 RplK 735450 rplK 888045 rplK Mycobacterium tuberculosis H37Rv 50S ribosomal protein L11 RplK NP_215154.1 735022 D 83332 CDS NP_215155.1 15607781 888043 735517..736224 1 NC_000962.3 Rv0641, (MTCY20H10.22), len: 235 aa. rplA, 50S ribosomal protein L1, equivalent to NP_302281.1|NC_002677 50S ribosomal protein L1 from Mycobacterium leprae (235 aa). Also highly similar to others e.g. P3625|RL1_STRGR 50s ribosomal protein L1 from Streptomyces griseus (240 aa),FASTA scores: opt: 1081, E(): 0, (72.2% identity in 230 aa overlap); etc. Belongs to the L1P family of ribosomal proteins.; 50S ribosomal protein L1 RplA 736224 rplA 888043 rplA Mycobacterium tuberculosis H37Rv 50S ribosomal protein L1 RplA NP_215155.1 735517 D 83332 CDS NP_215156.1 15607782 888056 complement(736298..737203) 1 NC_000962.3 Rv0642c, (MTCY20H10.23c), len: 301 aa. MmaA4,methoxy mycolic acid synthase 4 (methyltransferase) (see citations below). Equivalent to AAC44876|AAC44876.1|cmaA methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (298 aa); NP_302280.1|NC_002677 methyl mycolic acid synthase 4 from Mycobacterium leprae (298 aa); and highly similar to others from Mycobacteria e.g. downstream ORF P72027|mmaA3|Rv0643c|MTCY20H10.24c putative methoxy mycolic acid synthase 3 from Mycobacterium tuberculosis (293 aa). Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al.,2010).; Methoxy mycolic acid synthase 4 MmaA4 (methyl mycolic acid synthase 4) (MMA4) (hydroxy mycolic acid synthase) 737203 mmaA4 888056 mmaA4 Mycobacterium tuberculosis H37Rv Methoxy mycolic acid synthase 4 MmaA4 (methyl mycolic acid synthase 4) (MMA4) (hydroxy mycolic acid synthase) NP_215156.1 736298 R 83332 CDS NP_215157.1 15607783 888058 complement(737268..738149) 1 NC_000962.3 Rv0643c, (MTCY20H10.24c), len: 293 aa. MmaA3,methoxy mycolic acid synthase 3 (methyltransferase) (see citations below). Equivalent to AAC44875|AAC44875.1|cmaB methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (289 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa).; Methoxy mycolic acid synthase 3 MmaA3 (methyl mycolic acid synthase 3) (MMA3) (hydroxy mycolic acid synthase) 738149 mmaA3 888058 mmaA3 Mycobacterium tuberculosis H37Rv Methoxy mycolic acid synthase 3 MmaA3 (methyl mycolic acid synthase 3) (MMA3) (hydroxy mycolic acid synthase) NP_215157.1 737268 R 83332 CDS NP_215158.1 15607784 888061 complement(738297..739160) 1 NC_000962.3 Rv0644c, (MTCY20H10.25c), len: 287 aa. MmaA2,methoxy mycolic acid synthase 2 (methyltransferase) (see citations below). Equivalent to AAC44874|AAC44874.1|cmaC methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (287 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). Note that alternative start is at position 739247.; Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase) 739160 mmaA2 888061 mmaA2 Mycobacterium tuberculosis H37Rv Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase) NP_215158.1 738297 R 83332 CDS NP_215159.1 15607785 888060 complement(739327..740187) 1 NC_000962.3 Rv0645c, (MTCY20H10.26c), len: 286 aa. MmaA1,methoxy mycolic acid synthase 1 (methyltransferase) (see citations below). Equivalent to NP_302279.1|NC_002677 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa).; Methoxy mycolic acid synthase 1 MmaA1 (methyl mycolic acid synthase 1) (MMA1) (hydroxy mycolic acid synthase) 740187 mmaA1 888060 mmaA1 Mycobacterium tuberculosis H37Rv Methoxy mycolic acid synthase 1 MmaA1 (methyl mycolic acid synthase 1) (MMA1) (hydroxy mycolic acid synthase) NP_215159.1 739327 R 83332 CDS NP_215160.1 15607786 888065 complement(740234..741139) 1 NC_000962.3 Rv0646c, (MTCY20H10.27c), len: 301 aa. Probable lipG, lipase/esterase, equivalent to NP_302278.1|NC_002677 probable hydrolase from Mycobacterium leprae (304 aa). Also highly similar to various hydrolases, especially lipases e.g. AA61351.1|X88895 carboxyl esterase from Acinetobacter calcoaceticus (312 aa), FASTA scores: opt: 867, E(): 0,(50.2% identity in 279 aa overlap); etc. Also similar to transferases e.g. P77026 macrolide 2'-phosphotransferase II from Escherichia coli (279 aa), FASTA scores: E(): 1.3e-14,(32.5% identity in 286 aa overlap). Similar to M. tuberculosis non-heme bromoperoxidases and epoxide hydrolases.; Probable lipase/esterase LipG 741139 lipG 888065 lipG Mycobacterium tuberculosis H37Rv Probable lipase/esterase LipG NP_215160.1 740234 R 83332 CDS NP_215161.1 15607787 888070 complement(741151..742617) 1 NC_000962.3 Rv0647c, (MTCY20H10.28c), len: 488 aa. Conserved protein, equivalent to NP_302277.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (448 aa). Also showing similarity to a variety of hypothetical ABC1-like proteins or conserved hypothetical proteins e.g. D90908_28|P73627 ABC1-like protein from Synechocystis (585 aa), FASTA scores: E(): 1.8e-31, (29.1% identity in 474 aa overlap); Q55884 HYPOTHETICAL6 5.0 KD protein (567 aa),FASTA scores: opt: 583, E(): 5.7e-30, (28.1% identity in 416 aa overlap); etc. Also similar to Rv3197 conserved hypothetical protein from Mycobacterium tuberculosis.; hypothetical protein 742617 888070 Rv0647c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215161.1 741151 R 83332 CDS NP_215162.1 15607788 888048 742719..746366 1 NC_000962.3 Rv0648, (MTCY20H10.29), len: 1215 aa. Alpha-mannosidase (see citation below), showing some similarity to hypothetical proteins and various sugar hydrolases e.g. SYCSLRA_6|Q55528 hypothetical 1 20.4 kDa protein from Synechocystis (1042 aa), FASTA scores: opt: 260, E(): 3.6e-08, (23.4% identity in 602 aa overlap); etc. Contains PS00659 Glycosyl hydrolases family 5 signature.; Alpha-mannosidase 746366 888048 Rv0648 Mycobacterium tuberculosis H37Rv Alpha-mannosidase NP_215162.1 742719 D 83332 CDS YP_177744.1 57116766 888079 746363..747037 1 NC_000962.3 Rv0649, (MTCY20H10.30), len: 224 aa. Possible fabD2,malonyl CoA-acyl carrier protein transacylase, similar to mtfabd|FABD_MYCTU|Q10501|Rv2243 malonyl CoA-acyl carrier protein transacylase from Mycobacterium tuberculosis (302 aa), FASTA scores: opt: 133, E(): 0.074, (31.3% identity in 147 aa overlap).; Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT) 747037 fabD2 888079 fabD2 Mycobacterium tuberculosis H37Rv Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT) YP_177744.1 746363 D 83332 CDS NP_215164.1 15607790 888082 747037..747945 1 NC_000962.3 Rv0650, (MTCY20H10.31), len: 302 aa. Possible sugar kinase, highly similar to others e.g. CAB95296.1|AL359779 putative sugar kinase from Streptomyces coelicolor (317 aa); NP_406512.1|NC_003143 putative sugar kinase from Yersinia pestis (290 aa); NP_229269.1|NC_000853 glucokinase from Thermotoga maritima (317 aa); etc.Contains PS01125 ROK family signature. Belongs to the ROK (NAGC/XYLR) family.; Possible sugar kinase 747945 888082 Rv0650 Mycobacterium tuberculosis H37Rv Possible sugar kinase NP_215164.1 747037 D 83332 CDS NP_215165.1 15607791 888049 748276..748812 1 NC_000962.3 Rv0651, (MTCY20H10.32), len: 178 aa. rplJ, 50S ribosomal protein L10, equivalent to NP_302276.1|NC_002677 50S ribosomal protein L10 from Mycobacterium leprae (177 aa). Also highly similar to others e.g. P36257|RL10_STRGR 50s ribosomal protein L10 from Streptomyces griseus (185 aa), FASTA scores: opt: 633, E(): 0, (59.0 % identity in 173 aa overlap); etc. Belongs to the L10P family of ribosomal proteins.; 50S ribosomal protein L10 RplJ 748812 rplJ 888049 rplJ Mycobacterium tuberculosis H37Rv 50S ribosomal protein L10 RplJ NP_215165.1 748276 D 83332 CDS NP_215166.1 15607792 888078 748849..749241 1 NC_000962.3 Rv0652, (MTCY20H10.33), len: 130 aa. rplL (alternate gene name: L7|L12), 50S ribosomal protein L7/L12,equivalent to NP_302275.1|NC_002677 50S ribosomal protein L7/L12 from Mycobacterium leprae (130 aa); and P37381|RL7_MYCBO 50s ribosomal protein L7/L12 from Mycobacterium bovis (130 aa). Also highly similar to others e.g. P02396|RL7_STRGR 50S ribosomal protein L7/L12 from Streptomyces griseus (127 aa); etc. Belongs to the L12P family of ribosomal proteins.; 50S ribosomal protein L7/L12 RplL (SA1) 749241 rplL 888078 rplL Mycobacterium tuberculosis H37Rv 50S ribosomal protein L7/L12 RplL (SA1) NP_215166.1 748849 D 83332 CDS NP_215167.1 15607793 888087 complement(749234..749929) 1 NC_000962.3 Rv0653c, (MTCI376.23, MTCY20H10.34c), len: 231 aa. Possible transcriptional regulator, TetR family, similar in N-terminus to others e.g. CAC03642.1|AL391338 putative TetR-family transcriptional regulator from Streptomyces coelicolor (190 aa); Q51597 cam repressor from Pseudomonas putida (186 aa), FASTA scores: opt: 150, E(): 0.00085,(27.8% identity in 97 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis hypothetical transcriptional regulators Rv0681 and Rv1816. Contains probable helix-turn helix motif from aa 27-48 (Score 1156,+3.12 SD).; Possible transcriptional regulatory protein (probably TetR-family) 749929 888087 Rv0653c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably TetR-family) NP_215167.1 749234 R 83332 CDS NP_215168.1 15607794 888089 750000..751505 1 NC_000962.3 Rv0654, (MTCI376.22), len: 501 aa. Probable dioxygenase, highly similar to others eg AAK06796.1|AF324838_15|AF324838|SimC5 putative dioxygenase (involved in tetraene formation) from Streptomyces antibioticus (456 aa); CAB56138.1| AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); T51734 neoxanthin cleavage enzyme (9-cis-epoxy-carotenoid dioxygenase) from Arabidopsis thaliana (538 aa); Q53353 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis), FASTA scores: opt: 280, E(): 2.3e-11, (28.5% identity in 523 aa overlap); etc. Also some similarity with Rv0913c|MTCY21C12.07c possible dioxygenase from Mycobacterium tuberculosis (501 aa), FASTA score: (29.5% identity in 522 aa overlap).; Probable dioxygenase 751505 888089 Rv0654 Mycobacterium tuberculosis H37Rv Probable dioxygenase NP_215168.1 750000 D 83332 CDS NP_215169.1 15607795 888081 751517..752596 1 NC_000962.3 Rv0655, (MTCI376.21), len: 359 aa. Possible mkl,ribonucleotide-transport ATP-binding protein ABC transporter (see Braibant et al., 2000), equivalent to P30769|MKL_MYCLE|ML1892 possible ribonucleotide transport ATP-binding protein from Mycobacterium leprae (347 aa),FASTA scores: opt: 2021, E(): 0, (92.2% identity in 335 aa overlap). Also highly similar to many e.g. AB92896.1|AL356992 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (343 aa); NP_253146.1|NC_002516 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa (269 aa); P45393|YRBF_ECOLI hypothetical ABC transporter ATP-binding protein from Escherichia coli (269 aa), FASTA scores: opt: 644, E(): 3.4e-33, (38.5% identity in 244 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC transporters e.g. P71747|CYSA|Rv2397c|MTCY253.24 (351 aa),FASTA score: (33.6% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Possible ribonucleotide-transport ATP-binding protein ABC transporter Mkl 752596 mkl 888081 mkl Mycobacterium tuberculosis H37Rv Possible ribonucleotide-transport ATP-binding protein ABC transporter Mkl NP_215169.1 751517 D 83332 CDS NP_215170.1 15607796 888106 complement(752984..753367) 1 NC_000962.3 Rv0656c, (MTCI376.20), len: 127 aa. Possible vapC6,toxin, part of toxin-antitoxin (TA) operon with Rv0657c,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other proteins from Mycobacterium tuberculosis e.g. Rv2757c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC6 753367 vapC6 888106 vapC6 Mycobacterium tuberculosis H37Rv Possible toxin VapC6 NP_215170.1 752984 R 83332 CDS NP_215171.1 15607797 888077 complement(753462..753617) 1 NC_000962.3 Rv0657c, (MTCI376.19), len: 51 aa. Possible vapB6,antitoxin, part of toxin-antitoxin (TA) operon with Rv0656c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2009|MT2064.1|MTCY39.08c|YW08_MYCTU|Q10848 (80 aa), FASTA scores: opt: 107, E(): 0.0038, (45.8% identity in 48 aa overlap), Rv2871, Rv1560, etc. Also some similarity with AL020958|SC4H8_7 from Streptomyces coelicolor (66 aa),FASTA score: (41.0% identity in 39 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible antitoxin VapB6 753617 vapB6 888077 vapB6 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB6 NP_215171.1 753462 R 83332 CDS NP_215172.1 15607798 888102 complement(753693..754409) 1 NC_000962.3 Rv0658c, (MTCI376.18), len: 238 aa. Probable conserved integral membrane protein, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and showing some similarity with P33774|YPRB_ECOLI hypothetical 24.3 kDa protein from Escherichia coli (217 aa), FASTA scores: opt: 174, E(): 5.3e-05, (25.6% identity in 223 aa overlap). Also similar to Rv1863c and Rv0804 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable conserved integral membrane protein 754409 888102 Rv0658c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215172.1 753693 R 83332 CDS NP_215173.1 15607799 888134 complement(754685..754993) 1 NC_000962.3 Rv0659c, (MTCI376.17), len: 102 aa. MazF2, toxin,part of toxin-antitoxin (TA) operon with Rv0660c (See Pandey and Gerdes, 2005; Zhu et al., 2006), weakly similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1942c, Rv1495, etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Toxin MazF2 754993 mazF2 888134 mazF2 Mycobacterium tuberculosis H37Rv Toxin MazF2 NP_215173.1 754685 R 83332 CDS NP_215174.1 15607800 888141 complement(754980..755225) 1 NC_000962.3 Rv0660c, (MTCI376.16), len: 81 aa. Possible mazE2,antitoxin, part of toxin-antitoxin (TA) operon with Rv0659c (See Pandey and Gerdes, 2005; Zhu et al., 2006), showing some similarity to AF016485_130 from Halobacterium sp (100 aa), FASTA scores: (32.4% identity in 74 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible antitoxin MazE2 755225 mazE2 888141 mazE2 Mycobacterium tuberculosis H37Rv Possible antitoxin MazE2 NP_215174.1 754980 R 83332 CDS NP_215175.1 15607801 888143 complement(755335..755772) 1 NC_000962.3 Rv0661c, (MTCI376.15), len: 145 aa. Possible vapC7,toxin, part of toxin-antitoxin (TA) operon with Rv0662c,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2863|MTV003.09|MTV003_7 (126 aa), FASTA scores: E(): 0.00087, (30.4% identity in 125 aa overlap),Rv0749|MTV041.23 (163 aa); Rv0277c, Rv2530c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC7 755772 vapC7 888143 vapC7 Mycobacterium tuberculosis H37Rv Possible toxin VapC7 NP_215175.1 755335 R 83332 CDS NP_215176.2 448824747 888117 complement(755769..756023) 1 NC_000962.3 Rv0662c, (MTCI376.14), len: 84 aa. Possible vapB7,antitoxin, part of toxin-antitoxin (TA) operon with Rv0661c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2871, Rv1241, Rv2550c, etc. Start changed since first submission, now 38 aa shorter. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible antitoxin VapB7 756023 vapB7 888117 vapB7 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB7 NP_215176.2 755769 R 83332 CDS NP_215177.1 15607803 888144 756137..758500 1 NC_000962.3 Rv0663, (MTCI376.13c), len: 787 aa. Possible atsD,arylsulfatase, similar to others e.g. P5169|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 653, E(): 0, (33.1% identity in 544 aa overlap); etc. Also similar to P95059|MTCY210.30|ATSA|Rv0711|MTCY210.30 from Mycobacterium tuberculosis (787 aa), FASTA score: (38.9% identity in 769 aa overlap); and other arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c|ATSB (970 aa), Rv0711, etc. Contains PS00523 Sulfatases signature 1. Belongs to the sulfatase family. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase) 758500 atsD 888144 atsD Mycobacterium tuberculosis H37Rv Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase) NP_215177.1 756137 D 83332 CDS NP_215178.1 15607804 888146 758532..758804 1 NC_000962.3 Rv0664, (MTCI376.12c), len: 90 aa. Possible vapB8,antitoxin, part of toxin-antitoxin (TA) operon with Rv0665 (See Arcus et al., 2005; Pandey and Gerdes, 2005). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible antitoxin VapB8 758804 vapB8 888146 vapB8 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB8 NP_215178.1 758532 D 83332 CDS NP_215179.1 15607805 888149 758801..759139 1 NC_000962.3 Rv0665, (MTCI376.11c), len: 112 aa. Possible vapC8,toxin, part of toxin-antitoxin (TA) operon with Rv0664,contains PIN domain (See Arcus et al. 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0627 (135 aa), and Rv0595c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC8 759139 vapC8 888149 vapC8 Mycobacterium tuberculosis H37Rv Possible toxin VapC8 NP_215179.1 758801 D 83332 CDS NP_215180.1 15607806 888158 759136..759309 1 NC_000962.3 Rv0666, (MTCI376.10c), len: 57 aa. Possible membrane protein; has hydrophobic stretch at aa 29-47. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible membrane protein 759309 888158 Rv0666 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215180.1 759136 D 83332 CDS NP_215181.1 15607807 888164 759807..763325 1 NC_000962.3 Rv0667, (MTCI376.08c), len: 1172 aa. RpoB,DNA-directed RNA polymerase, beta chain (see Miller et al.,1994; Ahmad et al., 2000), equivalent to P30760|RPOB_MYCLE|ML1891 DNA-directed RNA polymerase beta chain from Mycobacterium leprae (1178 aa). Also highly similar to others e.g. AAF60349.1|AF242549_1|AF242549 DNA-dependent RNA polymerase beta subunit from Amycolatopsis mediterranei (1167 aa); CAB77428.1|AL160431 DNA-directed RNA polymerase beta chain from Streptomyces coelicolor (1161 aa); etc. Start site chosen on basis of RBS but alternative start exists at position 14359. Belongs to the RNA polymerase beta chain family.; DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) 763325 rpoB 888164 rpoB Mycobacterium tuberculosis H37Rv DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) NP_215181.1 759807 D 83332 CDS NP_215182.1 15607808 888177 763370..767320 1 NC_000962.3 Rv0668, (MTCI376.07c), len: 1316 aa. RpoC,DNA-directed RNA polymerase, beta' chain (see Miller et al., 1994), equivalent to P30761|RPOC_MYCLE|ML1890|S31146 DNA-directed RNA polymerase beta' chain from Mycobacterium leprae (1316 aa), FASTA scores: opt: 8295, E(): 0, (95.6% identity in 1316 aa overlap). Also highly similar to others e.g. CAB77429.1|AL160431 DNA-directed RNA polymerase beta' chain (fragment) from Streptomyces coelicolor (1059 aa); P37871|RPOC_BACSU from Bacillus subtilis (1199 aa), FASTA scores: opt: 2367, E(): 0, (52.9 identity in 1317 aa overlap); etc. Belongs to the RNA polymerase beta' chain family.; DNA-directed RNA polymerase (beta' chain) RpoC (transcriptase beta' chain) (RNA polymerase beta' subunit) 767320 rpoC 888177 rpoC Mycobacterium tuberculosis H37Rv DNA-directed RNA polymerase (beta' chain) RpoC (transcriptase beta' chain) (RNA polymerase beta' subunit) NP_215182.1 763370 D 83332 CDS NP_215183.1 15607809 888181 complement(767684..769597) 1 NC_000962.3 Rv0669c, (MTCI376.05), len: 637 aa. Possible hydrolase, highly similar to various hydrolases (N-terminus shorter) e.g. BAA88409.1|AB028646 alkaline ceramidase from Pseudomonas aeruginosa (670 aa,) FASTA scores: opt: 1490,E(): 0, (41.2% identity in 651 aa overlap); NP_063946.1|NM_019893 mitochondrial ceramidase from Homo sapiens (761 aa); P_446098.1|NM_053646 N-acylsphingosine amidohydrolase 2 from Rattus norvegicus (761 aa); BAB09641.1|AB016885 neutral ceramidase from Arabidopsis thaliana (705 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible hydrolase 769597 888181 Rv0669c Mycobacterium tuberculosis H37Rv Possible hydrolase NP_215183.1 767684 R 83332 CDS NP_215184.1 15607810 888190 769792..770550 1 NC_000962.3 Rv0670, (MTCI376.04c), len: 252 aa. Probable end (alternate gene name: nfo), endonuclease IV (apurinase) (see citation below), equivalent to END_MYCLE|P30770|NFO|ML1889 probable endonuclease IV (apurinase) from Mycobacterium leprae (252 aa), FASTA scores: opt: 1463, E(): 0, (85.6% identity in 250 aa overlap). Also similar to others e.g. Q9S2N2|END4_STRCO|NFO|SC6E10.05 probable endonuclease IV from Streptomyces coelicolor (294 aa); etc. Contains PS00729 AP endonucleases family 2 signatures 1 and 2 (PS00729, and PS00730). Belongs to the AP endonucleases family 2. Cofactor: binds 3 zinc ions. The transcription of this CDS seems negatively regulated by the product of mce2R|Rv0586 (See Santangelo et al., 2009).; Probable endonuclease IV End (endodeoxyribonuclease IV) (apurinase) 770550 end 888190 end Mycobacterium tuberculosis H37Rv Probable endonuclease IV End (endodeoxyribonuclease IV) (apurinase) NP_215184.1 769792 D 83332 CDS NP_215185.1 15607811 888194 770582..771424 1 NC_000962.3 Rv0671, (MTCI376.03c), len: 280 aa. Possible lpqP,conserved lipoprotein, similar to U00012|B1308_F2_43|Q49658 from Mycobacterium leprae (302 aa), FASTA scores: opt: 449,E(): 2.4e-22, (37.6% identity in 242 aa overlap). Also highly similar to lpqC|Rv3298c|MTCY71.38c putative lipoprotein from Mycobacterium tuberculosis (304 aa). Also similar to a large variety of proteins including various esterases and poly(3-hydroxyalkanoate) depolymerases, e.g. NP_249234.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (322 aa); C-terminus of AAD45376.1|AF164516_1|AF164516 cinnamoyl ester hydrolase EstA from Piromyces equi (536 aa); part of P52090|PHA1_PSELE poly(3-hydroxyalkanoate) depolymerase C precursor from Pseudomonas lemoignei (414 aa); CAC10310.1|AL442629 putative secreted protein from Streptomyces coelicolor (348 aa); etc. Has a 17 aa signal sequence and contains appropriately positioned (PS00013) Prokaryotic membrane lipoprotein lipid attachment site.; Possible conserved lipoprotein LpqP 771424 lpqP 888194 lpqP Mycobacterium tuberculosis H37Rv Possible conserved lipoprotein LpqP NP_215185.1 770582 D 83332 CDS NP_215186.1 15607812 888198 771484..773112 1 NC_000962.3 Rv0672, (MTCI376.02c), len: 542 aa. Probable fadE8,acyl-CoA dehydrogenase, highly similar to many e.g. CAC33951.1|AL589708 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (557 aa); P33224|AIDB_ECOLI|B4187 aidb protein (acyl-CoA dehydrogenases family) from Escherichia coli strain K12 (546 aa), FASTA scores: opt: 1369, E(): 0, (44.1% identity in 524 aa overlap); etc. Also similar to several other M. tuberculosis proteins e.g. Rv0154c|MTCI5.28c FASTA score: (26.3% identity in 342 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073). Belongs to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE8 773112 fadE8 888198 fadE8 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE8 NP_215186.1 771484 D 83332 CDS NP_215187.1 15607813 888175 773123..774061 1 NC_000962.3 Rv0673, (MTCI376.01c, MTV040.01), len: 312 aa. Possible echA4, enoyl-CoA hydratase, showing similarity with others e.g. NP_419216.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (256 aa); Q52995|ECHH_RHIME probable enoyl-CoA hydratase from Sinorhizobium meliloti (257 aa), FASTA scores: opt: 210, E(): 1.2e-06, (27.9% identity in 280 aa overlap); etc. Also similar to other enoyl-CoA hydratases from Mycobacterium tuberculosis e.g. P95279|MTCY09F9.29|ECHA13|Rv1935c|MTCY09F9.29 enoyl-CoA hydratase (318 aa), FASTA score: (27.1% identity in 280 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible enoyl-CoA hydratase EchA4 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 774061 echA4 888175 echA4 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase EchA4 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215187.1 773123 D 83332 CDS NP_215188.1 15607814 888203 774064..774786 1 NC_000962.3 Rv0674, (MTV040.02), len: 240 aa. Conserved hypothetical protein, highly similar to AC13063.1|AL445503 conserved hypothetical protein from Streptomyces coelicolor (268 aa); and similar to NP_438100.1|NC_003078 putative regulator of phenylacetic acid degradation ArsR family protein from Sinorhizobium meliloti (306 aa) and other proteins e.g. AB011837|AB011837_13 hypothetical protein from Bacillus halodurans (298 aa), FASTA scores: opt: 148,E(): 0.0081, (25.1% identity in 235 aa overlap); etc.; hypothetical protein 774786 888203 Rv0674 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215188.1 774064 D 83332 CDS YP_177745.1 57116767 888222 774783..775574 1 NC_000962.3 Rv0675, (MTV040.03), len: 263 aa. Probable echA5,enoyl-CoA hydratase, similar to several e.g. NP_252116.1|NC_002516 probable enoyl CoA-hydratase/isomerase from Pseudomonas aeruginosa (256 aa); Q20376 protein similar to enoyl-CoA hydratase from Caenorhabditis elegans (258 aa), FASTA scores: opt: 697,E(): 0, (47.3% identity in 245 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Z92669|MTCY8D5_17 (262 aa), FASTA scores: opt: 493, E(): 3.6e-25, (39.1% identity in 243 aa overlap); etc.; Probable enoyl-CoA hydratase EchA5 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 775574 echA5 888222 echA5 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA5 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) YP_177745.1 774783 D 83332 CDS NP_215190.1 15607816 888219 complement(775586..778480) 1 NC_000962.3 Rv0676c, (MTV040.04c), len: 964 aa. Probable mmpL5,conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. MTV037_14, MTCY98_8,MTCY20G9_34, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19,etc. Also similar to other Mycobacterial mmpl proteins e.g. P54881|MML4_MYCLE putative membrane protein MMPL4 from Mycobacterium leprae (959 aa), FASTA scores: opt: 3991,E(): 0, (62.8% identity in 933 aa overlap); etc. Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL5 778480 mmpL5 888219 mmpL5 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL5 NP_215190.1 775586 R 83332 CDS NP_215191.1 15607817 888233 complement(778477..778905) 1 NC_000962.3 Rv0677c, (MTV040.05c), len: 142 aa. Possible mmpS5,conserved membrane protein (see Tekaia et al., 1999),highly similar to other Mycobacterial proteins e.g. P54880|MMS4_MYCLE putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 443, E(): 1.4e-23, (47.1% identity in 155 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved membrane protein MmpS5 778905 mmpS5 888233 mmpS5 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein MmpS5 NP_215191.1 778477 R 83332 CDS NP_215192.1 15607818 888235 778990..779487 1 NC_000962.3 Rv0678, (MTV040.06), len: 165 aa. Conserved protein,showing weak similarity with AL049754|SCH10_10 hypothetical protein from Streptomyces coelicolor (152 aa), FASTA scores: opt: 149, E(): 0.0018, (22.9% identity in 140 aa overlap).; hypothetical protein 779487 888235 Rv0678 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215192.1 778990 D 83332 CDS NP_215193.1 15607819 888230 complement(779543..780040) 1 NC_000962.3 Rv0679c, (MTV040.07c), len: 165 aa. Conserved Thr-rich protein, similar in part to neighboring ORF Rv0680c (124 aa), FASTA score: (35.1% identity in 131 aa overlap); and Rv0314c (220 aa). Predicted to be an outer membrane protein (See Song et al., 2008).; Conserved threonine rich protein 780040 888230 Rv0679c Mycobacterium tuberculosis H37Rv Conserved threonine rich protein NP_215193.1 779543 R 83332 CDS NP_215194.1 15607820 888229 complement(780042..780416) 1 NC_000962.3 Rv0680c, (MTV040.08c), len: 124 aa. Possible conserved transmembrane protein, showing similarity with C-terminal part of Rv0314c|Z96800|MTCY63.19c conserved hypothetical protein from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 175, E(): 2.2e-05, (31.4% identity in 102 aa overlap). Also some similarity to upstream ORF Rv0679c|MTV040.07c conserved hypothetical threonine rich protein (124 aa), FASTA score: (35.1% identity in 131 aa overlap). Contains possible membrane spanning regions.; Probable conserved transmembrane protein 780416 888229 Rv0680c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215194.1 780042 R 83332 CDS NP_215195.1 15607821 888239 780721..781311 1 NC_000962.3 Rv0681, (MTV040.09), len: 196 aa. Probable transcription regulator, TetR family, similar to others and especially many tetracycline repressors e.g. T34657 probable transcription regulator from Streptomyces coelicolor (189 aa); AF0278|AF027868_40|NP_389788.1|NC_000964 yobS regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 213,E(): 1.6e-07, (28.8% identity in 153 aa overlap); P09164|TER4_ECOLI tetracycline repressor protein from Escherichia coli (217 aa), FASTA scores: opt: 145, E(): 0.0068, (39.0% identity in 59 aa overlap); etc. Contains helix-turn-helix motif at aa 28-49 (Score 1020, +2.66 SD).; Probable transcriptional regulatory protein (possibly TetR-family) 781311 888239 Rv0681 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (possibly TetR-family) NP_215195.1 780721 D 83332 CDS NP_215196.1 15607822 888259 781560..781934 1 NC_000962.3 Rv0682, (MTV040.10), len: 124 aa. rpsL, 30S ribosomal protein S12 (see citations below), equivalent to others from Mycobacteria e.g. P41195|RS12_MYCSM 30S ribosomal protein S12 from Mycobacterium smegmatis (124 aa); P51999|RS12_MYCAV 30S ribosomal protein S12 from Mycobacterium avium (124 aa); etc. Also highly similar to others from other organisms e.g. P97222|RS12_STRCO 30S ribosomal protein S12 from Streptomyces roseosporus,lividans and coelicolor (123 aa); etc. Contains PS00055 Ribosomal protein S12 signature. Belongs to the S12P family of ribosomal proteins. Nucleotide position 781922 in the genome sequence has been corrected, A:G resulting in K121K.; 30S ribosomal protein S12 RpsL 781934 rpsL 888259 rpsL Mycobacterium tuberculosis H37Rv 30S ribosomal protein S12 RpsL NP_215196.1 781560 D 83332 CDS NP_215197.1 15607823 888245 781934..782404 1 NC_000962.3 Rv0683, (MTV040.11), len: 156 aa. rpsG, 30S ribosomal protein S7 (see citation below), equivalent to others from Mycobacteria e.g. P41193|RS7_MYCSM 30S ribosomal protein S7 from Mycobacterium smegmatis (156 aa),FASTA scores: opt: 986, E(): 0, (96.2% identity in 156 aa overlap); Q53539|RS7_MYCBO 30S ribosomal protein S7 from Mycobacterium bovis (156 aa); etc. Also highly similar to others e.g. Q9L0K4|RS7_STRCO 30S ribosomal protein S7 from Streptomyces coelicolor (156 aa); etc. Contains PS00052 Ribosomal protein S7 signature. Belongs to the S7P family of ribosomal proteins.; 30S ribosomal protein S7 RpsG 782404 rpsG 888245 rpsG Mycobacterium tuberculosis H37Rv 30S ribosomal protein S7 RpsG NP_215197.1 781934 D 83332 CDS YP_177746.1 57116768 888240 782485..784590 1 NC_000962.3 Rv0684, (MTV040.12, MTCY210.01), len: 701 aa. Probable fusA1, elongation factor G, equivalent to P30767|EFG_MYCLE|S31150 translation elongation factor EF-G from Mycobacterium leprae (701 aa), FASTA scores: opt: 2521, E(): 0, (88.2% identity in 432 aa overlap). Also highly similar to others e.g. CAB81852.1|AL161691 elongation factor G from Streptomyces coelicolor (708 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family,EF-G/EF-2 subfamily. Note that previously known as fusA.; Probable elongation factor G FusA1 (EF-G) 784590 fusA1 888240 fusA1 Mycobacterium tuberculosis H37Rv Probable elongation factor G FusA1 (EF-G) YP_177746.1 782485 D 83332 CDS NP_215199.1 15607825 888262 784821..786011 1 NC_000962.3 Rv0685, (MTCY210.02), len: 396 aa. Probable tuf,iron-regulated elongation factor EF-Tu, equivalent to JC2262 translation elongation factor Tu from Mycobacterium leprae (396 aa). Also highly similar to others e.g. P42439|EFTU_CORGL elongation factor TU (EF-TU) from Corynebacterium glutamicum (396 aa); etc. Contains PS00017 ATP/GTP-binding site motif A, and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, EF-TU/EF-1A subfamily. Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable iron-regulated elongation factor TU Tuf (EF-TU) 786011 tuf 888262 tuf Mycobacterium tuberculosis H37Rv Probable iron-regulated elongation factor TU Tuf (EF-TU) NP_215199.1 784821 D 83332 CDS NP_215200.1 15607826 888271 786149..786946 1 NC_000962.3 Rv0686, (MTCY210.03), len: 265 aa. Probable membrane protein, with hydrophobic N-terminus.; Probable membrane protein 786946 888271 Rv0686 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_215200.1 786149 D 83332 CDS NP_215201.1 15607827 888279 787099..787926 1 NC_000962.3 Rv0687, (MTCY210.04), len: 275 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases (generally SDR family) e.g. U17129|RSU17129_7 short-chain dehydrogenase from Rhodococcus erythropolis (275 aa), FASTA scores: opt: 1112,E(): 0, (61.2% identity in 268 aa overlap); MMU34072_2 steroid dehydrogenase from Musmus culus (260 aa), FASTA scores: opt: 390, E(): 2.2e-17, (34.1% identity in 267 aa overlap); etc. Also similar to MTV002_16|O33292|Rv2750 dehydrogenase from Mycobacterium tuberculosis (272 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature.; Probable short-chain type dehydrogenase/reductase 787926 888279 Rv0687 Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_215201.1 787099 D 83332 CDS NP_215202.1 15607828 888280 787940..789160 1 NC_000962.3 Rv0688, (MTCY210.05), len: 406 aa. Putative ferredoxin reductase, highly similar to others e.g. BAB55881.1|AB054975 ferredoxin reductase from Terrabacter sp. DBF63 (410 aa); CAC04223.1|AL391515 putative ferredoxin reductase from Streptomyces coelicolor (420 aa); PPU24215_8|Q51973 P-cumate dioxygenase ferredoxin reductase subunit from Pseudomonas putida (402 aa), FASTA scores: opt: 738, E(): 0, (38.8% identity in 330 aa overlap); etc. Also similar to Rv0253 and Rv1869c from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family.; Putative ferredoxin reductase 789160 888280 Rv0688 Mycobacterium tuberculosis H37Rv Putative ferredoxin reductase NP_215202.1 787940 D 83332 CDS NP_215203.1 15607829 888283 complement(789157..789411) 1 NC_000962.3 Rv0689c, (MTCY210.06c), len: 84 aa. Hypothetical unknown protein.; Hypothetical protein 789411 888283 Rv0689c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215203.1 789157 R 83332 CDS NP_215204.1 15607830 888292 complement(790024..791073) 1 NC_000962.3 Rv0690c, (MTCY210.07c), len: 349 aa. Conserved hypothetical protein, showing similarity with NP_386956.1|NC_003047 conserved hypothetical protein from Sinorhizobium meliloti (358 aa); NP_356573.1|NC_003063 AGR_L_1570p from Agrobacterium tumefaciens (346 aa); NP_421938.1|NC_002696 conserved hypothetical protein from Caulobacter crescentus (370 aa).; hypothetical protein 791073 888292 Rv0690c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215204.1 790024 R 83332 CDS NP_215205.1 15607831 888296 complement(791070..791666) 1 NC_000962.3 Rv0691c, (MTCY210.08c), len: 198 aa. Probable transcriptional regulator, highly similar to AAC77476.1|U17129 unknown protein from Rhodococcus erythropolis (185 aa); and showing similarity with putative regulatory proteins eg STMTCREP_1|TCMR_STRGA|P39885 tetracenomycin c transcriptional repressor from Streptomyces glaucescens (226 aa), FASTA scores: opt: 178,E(): 8.5e-06, (27.9% identity in 201 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and probable helix-turn helix motifs from aa 34-55 (Score 1100,+2.93 SD) and 151-172 (Score 1124, +3.02 SD).; Probable transcriptional regulatory protein 791666 888296 Rv0691c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215205.1 791070 R 83332 CDS YP_007409348.1 448815475 14515851 791658..791846 1 NC_000962.3 Rv0691A, len: 62 aa. Mycofactocin precursor protein.; Mycofactocin precursor protein 791846 14515851 Rv0691A Mycobacterium tuberculosis H37Rv Mycofactocin precursor protein YP_007409348.1 791658 D 83332 CDS NP_215206.1 15607832 888285 791831..792160 1 NC_000962.3 Rv0692, (MTCY210.09), len: 109 aa. Conserved hypothetical protein, highly similar to U17129|RSU17129_3|AAC77477.1 unknown protein from Rhodococcus erythropolis (95 aa), FASTA scores: opt: 393,E(): 8.8e-22, (68.2% identity in 88 aa overlap).; hypothetical protein 792160 888285 Rv0692 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215206.1 791831 D 83332 CDS NP_215207.1 15607833 888302 792157..793332 1 NC_000962.3 Rv0693, (MTCY210.10), len: 391 aa. Probable pqqE (alternate gene name: pqqIII), coenzyme PQQ synthesis protein E, similar to others AE001109_9|O30258|PQQE coenzyme PQQ synthesis protein from Archaeoglobus fulgidus (375 aa), FASTA scores: E(): 1.6e-16, (28.1% identity in 377 aa overlap); PQQE_ACICA|P07782 coenzyme pqq synthesis protein e from Acinetobacter calcoaceticus (384 aa), FASTA scores: opt: 302, E(): 1.8e-12, (23.9% identity in 377 aa overlap); etc. Also similar to C-terminus of heme biosynthesis proteins e.g. O28270|AF2009 heme biosynthesis protein (NIRJ-2) from Archaeoglobus fulgidus (468 aa). Note that also highly similar to U17129|RSU17129_4|AAC77478.1 unknown protein from Rhodococcus erythropolis (405 aa),FASTA scores: opt: 1997, E(): 0, (73.3% identity in 390 aa overlap). Could belong to the MoaA / NifB / PqqE family.; Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III) 793332 pqqE 888302 pqqE Mycobacterium tuberculosis H37Rv Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III) NP_215207.1 792157 D 83332 CDS NP_215208.1 15607834 888310 793335..794525 1 NC_000962.3 Rv0694, (MTCY210.11), len: 396 aa. Possible lldD1,L-lactate dehydrogenase (cytochrome), similar to NP_302368.1|NC_002677 L-lactate dehydrogenase from Mycobacterium leprae (414 aa). Also similar to others e.g. NP_384560.1|NC_003047 putative L-lactate dehydrogenase (cytochrome) protein from Sinorhizobium meliloti (403 aa); NP_251072.1|NC_002516 L-lactate dehydrogenase from Pseudomonas aeruginosa (383 aa); P33232|LLDD_ECOLI L-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA scores: opt: 697, E(): 0, (34.5 identity in 380 aa overlap); etc; and also similar to other oxidoreductases. Note that also highly similar to RSU17129_5|AAC77479.1|U17129 unknown protein from Rhodococcus erythropolis (392 aa), FASTA scores: opt: 2006,E(): 0, (74.1% identity in 386 aa overlap). Also similar to lldD2|Rv1872c|MTCY180.46|MTCY359.01 possible L-lactate dehydrogenase (cytochrome) from Mycobacterium tuberculosis (414 aa). Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenases family.; Possible L-lactate dehydrogenase (cytochrome) LldD1 794525 lldD1 888310 lldD1 Mycobacterium tuberculosis H37Rv Possible L-lactate dehydrogenase (cytochrome) LldD1 NP_215208.1 793335 D 83332 CDS NP_215209.1 15607835 888314 794715..795470 1 NC_000962.3 Rv0695, (MTCY210.12), len: 251 aa. Conserved hypothetical protein, similar to many creatinine amidohydrolases or hypothetical proteins e.g. NP_443048.1|NC_000911 creatinine amidohydrolase from Synechococcus sp. PCC 6803 (273 aa); NP_466169.1|NC_003210 protein similar to creatinine amidohydrolase from Listeria monocytogenes (249 aa); T35153|SC5A7.04c hypothetical protein from Streptomyces coelicolor (273 aa); etc. Note that highly similar to RSU17129_10|AAC77474.1|U17129 unknown protein from Rhodococcus erythropolis (230 aa),FASTA scores: opt: 693, E(): 0, (55.7% identity in 237 aa overlap).; hypothetical protein 795470 888314 Rv0695 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215209.1 794715 D 83332 CDS NP_215210.1 15607836 888307 795519..796931 1 NC_000962.3 Rv0696, (MTCY210.13), len: 470 aa. Probable membrane sugar transferase, similar (except in N-terminus) to NP_069157.1|NC_000917 glycosyl transferase from Archaeoglobus fulgidus (324 aa); NP_279985.1|NC_002607 rhamnosyl transferase from Halobacterium sp. NRC-1 (299 aa); NP_059113.1|NM_017417 polypeptide N-acetylgalactosaminyltransferase 8 from (637 aa). Note that also highly similar to P46370|YTH1_RHOER hypothetical 55.3 KDA protein from Rhodococcus erythropolis (513 aa),FASTA scores: opt: 1514, E(): 0, (51.8% identity in 469 aa overlap).; Probable membrane sugar transferase 796931 888307 Rv0696 Mycobacterium tuberculosis H37Rv Probable membrane sugar transferase NP_215210.1 795519 D 83332 CDS NP_215211.1 15607837 888316 796933..798372 1 NC_000962.3 Rv0697, (MTCY210.14, unknown), len: 479 aa. Probable dehydrogenase, highly similar to P30772|YTUR_MYCLE hypothetical 24 kDa protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 557, E(): 1.7e-28, (46.2% identity in 223 aa overlap). Also highly similar to P46371|YTH2_RHOER hypothetical 53.0 KDA GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa); and similar to many dehydrogenases e.g. NP_250814.1|NC_002516 probable dehydrogenase from Pseudomonas aeruginosa (545 aa); BAA13145.1|D86622 FAD dependent L-sorbose dehydrogenase from Gluconobacter oxydans (531 aa); etc. Also similar to Rv1279 conserved hypothetical protein from Mycobacterium tuberculosis.; Probable dehydrogenase 798372 888316 Rv0697 Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_215211.1 796933 D 83332 CDS NP_215212.1 15607838 888327 798833..799444 1 NC_000962.3 Rv0698, (MTCY210.15), len: 203 aa. Conserved hypothetical protein, highly similar to C-terminus of Rv3639c|MTY15C10.12 conserved hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 2.1e-07, (54.8% identity in 73 aa overlap).; hypothetical protein 799444 888327 Rv0698 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215212.1 798833 D 83332 CDS NP_215213.1 15607839 888335 799629..799850 1 NC_000962.3 Rv0699, (MTCY210.17), len: 73 aa. Hypothetical unknown protein.; Hypothetical protein 799850 888335 Rv0699 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215213.1 799629 D 83332 CDS NP_215214.1 15607840 888331 800487..800792 1 NC_000962.3 Rv0700, (MTCY210.19), len: 101 aa. rpsJ (alternate gene name: nusE), 30S ribosomal protein S10 (see Gopal et al., 2001), equivalent to RS10_MYCLE P307653 30S ribosomal protein S10 from Mycobacterium leprae (101 aa), FASTA scores: opt: 645, E(): 0, (97.0% identity in 101 aa overlap). Also highly similar to others e.g. CAB82069.1|AL161803 30S ribosomal protein S10 from Streptomyces coelicolor (102 aa); etc. Contains PS00361 Ribosomal protein S10 signature. Belongs to the S10P family of ribosomal proteins.; 30S ribosomal protein S10 RpsJ (transcription antitermination factor NusE) 800792 rpsJ 888331 rpsJ Mycobacterium tuberculosis H37Rv 30S ribosomal protein S10 RpsJ (transcription antitermination factor NusE) NP_215214.1 800487 D 83332 CDS NP_215215.1 15607841 888343 800809..801462 1 NC_000962.3 Rv0701, (MTCY210.20), len: 217 aa. rplC, 50S ribosomal protein L3, equivalent to O06044|RL3_MYCBO 50S ribosomal protein L3 from Mycobacterium bovis BCG (217 aa); and P30762|RL3_MYCLE 50S ribosomal protein L3 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. CAB82070.1|AL161803 50S ribosomal protein L3 from Streptomyces coelicolor (214 aa); P52860|RL3_THETH ribosomal protein l3 from Thermus aquaticus (206 aa), FASTA scores: opt: 717, E(): 0, (55.2% identity in 210 aa overlap); etc. Contains PS00474 Ribosomal protein L3 signature. Belongs to the L3P family of ribosomal proteins.; 50S ribosomal protein L3 RplC 801462 rplC 888343 rplC Mycobacterium tuberculosis H37Rv 50S ribosomal protein L3 RplC NP_215215.1 800809 D 83332 CDS NP_215216.1 15607842 888345 801462..802133 1 NC_000962.3 Rv0702, (MTCY210.21), len: 223 aa. rplD, 50S ribosomal protein L4, equivalent to O06045|RL4_MYCBO 50S ribosomal protein L4 from Mycobacterium bovis BCG (223 aa); O06114|RL4_MYCSM 50S ribosomal protein L4 from Mycobacterium smegmatis (215 aa); and MLCB2492_3 50S ribosomal protein L4 from Mycobacterium leprae (230 aa). Also highly similar to others e.g. CAB82071.1|AL161803 50S ribosomal protein L4 from Streptomyces coelicolor (219 aa); P28601|RL4_BACST 50s ribosomal protein L4 from Bacillus stearothermophilus (207 aa), FASTA scores: opt: 522, E(): 3.5e-26, (42.4% identity in 198 aa overlap); etc. Belongs to the L4P family of ribosomal proteins.; 50S ribosomal protein L4 RplD 802133 rplD 888345 rplD Mycobacterium tuberculosis H37Rv 50S ribosomal protein L4 RplD NP_215216.1 801462 D 83332 CDS NP_215217.1 15607843 888353 802133..802435 1 NC_000962.3 Rv0703, (MTCY21.22), len: 100 aa. rplW, 50S ribosomal protein L23, equivalent to O06046|RL23_MYCBO 50S ribosomal protein L23 from Mycobacterium bovis BCG (100 aa); and MLCB2492_4 50S ribosomal protein L23 from Mycobacterium leprae (100 aa). Also highly similar to others e.g. CAB82072.1|AL161803 50S ribosomal protein L23 from Streptomyces coelicolor (139 aa) (N-terminus longer); P04454|RL23_BACST 50s ribosomal protein L23 from Bacillus stearothermophilus (95 aa), FASTA scores: opt: 275, E(): 1.4e-13, (50.5% identity in 95 aa overlap); etc. Contains PS00050 Ribosomal protein L23 signature. Belongs to the L23P family of ribosomal proteins.; 50S ribosomal protein L23 RplW 802435 rplW 888353 rplW Mycobacterium tuberculosis H37Rv 50S ribosomal protein L23 RplW NP_215217.1 802133 D 83332 CDS NP_215218.1 15607844 888341 802528..803370 1 NC_000962.3 Rv0704, (MTCY210.23), len: 280 aa. rplB, 50S ribosomal protein L2, equivalent to O06047|RL2_MYCBO 50S ribosomal protein L2 from Mycobacterium bovis BCG (280 aa); and MLCB2492_5M 50S ribosomal protein L2 from Mycobacterium leprae (280 aa). Also highly similar to others e.g. CAB82073.1|AL161803 50S ribosomal protein L2 from Streptomyces coelicolor (278 aa); P42919|RL2_BACSU 50s ribosomal protein l2 (bl2) from Bacillus subtilis (276 aa),FASTA scores: opt: 1179, E(): 0, (61.1% identity in 275 aa overlap); etc. Contains PS00467 Ribosomal protein L2 signature. Belongs to the L2P family of ribosomal proteins.; 50S ribosomal protein L2 RplB 803370 rplB 888341 rplB Mycobacterium tuberculosis H37Rv 50S ribosomal protein L2 RplB NP_215218.1 802528 D 83332 CDS NP_215219.1 15607845 888356 803411..803692 1 NC_000962.3 Rv0705, (MTCY210.24), len: 93 aa. rpsS, 30S ribosomal protein S19, equivalent to S36895 ribosomal protein S19 from Mycobacterium bovis (93 aa), FASTA scores: opt: 623, E(): 0, (98.9% identity in 93 aa overlap); and NP_302261.1|NC_002677 30S ribosomal protein S19 from Mycobacterium leprae (93 aa). Also highly similar to others e.g. CAB82074.1|AL161803 30S ribosomal protein S19 from Streptomyces coelicolor (93 aa); etc. Contains PS00323 Ribosomal protein S19 signature. Belongs to the S19P family of ribosomal proteins.; 30S ribosomal protein S19 RpsS 803692 rpsS 888356 rpsS Mycobacterium tuberculosis H37Rv 30S ribosomal protein S19 RpsS NP_215219.1 803411 D 83332 CDS NP_215220.1 15607846 888359 803689..804282 1 NC_000962.3 Rv0706, (MTCY210.25), len: 197 aa. rplV, 50S ribosomal protein L22, equivalent to O06115|RL22_MYCSM 50S ribosomal protein L22 from Mycobacterium smegmatis (153 aa); MBS10OPER_7 50S ribosomal protein L22 from Mycobacterium bovis BCG; and MLCB2492_7 50S ribosomal protein L22 from Mycobacterium leprae (175 aa). Also highly similar to others e.g. CAB82075.1|AL161803 50S ribosomal protein L22 from Streptomyces coelicolor (125 aa); P42060|RL22_BACSU 50s ribosomal protein L22 from Bacillus subtilis (113 aa), FASTA scores: opt: 368, E(): 2.4e-13,(52.8% identity in 108 aa overlap); etc. Contains PS00464 Ribosomal protein L22 signature, and contains repetitive sequence at C-terminus. Belongs to the L22P family of ribosomal proteins.; 50S ribosomal protein L22 RplV 804282 rplV 888359 rplV Mycobacterium tuberculosis H37Rv 50S ribosomal protein L22 RplV NP_215220.1 803689 D 83332 CDS NP_215221.1 15607847 888357 804282..805106 1 NC_000962.3 Rv0707, (MTCY210.26), len: 274 aa. rpsC, 30S ribosomal protein S3, equivalent to O06048|RS3_MYCBO|MBS10OPER_8 30S ribosomal protein S3 from Mycobacterium bovis BCG (274 aa); and MLCB2492_8 30S ribosomal protein S3 from Mycobacterium leprae (281 aa). Also highly similar to others e.g. CAB82076.1|AL161803 30S ribosomal protein S3 from Streptomyces coelicolor (277 aa); P21465|RS3_BACSU 30s ribosomal protein s3 (bs3) (bs2) from Bacillus subtilis (217 aa), FASTA scores: opt: 794, E(): 0,(52.8% identity in 212 aa overlap); etc. Belongs to the S3P family of ribosomal proteins.; 30S ribosomal protein S3 RpsC 805106 rpsC 888357 rpsC Mycobacterium tuberculosis H37Rv 30S ribosomal protein S3 RpsC NP_215221.1 804282 D 83332 CDS NP_215222.1 15607848 888377 805110..805526 1 NC_000962.3 Rv0708, (MTCY210.27), len: 138 aa. rplP, 50S ribosomal protein L16, equivalent to O06049|RL16_MYCBO|MBS10OPER_9 50S ribosomal protein L16 from Mycobacterium bovis BCG (138 aa); and MLCB2492_9 50S ribosomal protein L16 from Mycobacterium leprae (138 aa). Also highly similar to others e.g. CAB82077.1|AL161803 50S ribosomal protein L16 from Streptomyces coelicolor (139 aa); P14577|RL16_BACSU 50s ribosomal protein l16 from Bacillus subtilis (144 aa), FASTA scores: opt: 600, E(): 0,(63.2% identity in 136 aa overlap); etc. Contains PS00701 Ribosomal protein L16 signature 2. Belongs to the L16P family of ribosomal proteins.; 50S ribosomal protein L16 RplP 805526 rplP 888377 rplP Mycobacterium tuberculosis H37Rv 50S ribosomal protein L16 RplP NP_215222.1 805110 D 83332 CDS NP_215223.1 15607849 888374 805526..805759 1 NC_000962.3 Rv0709, (MTCY210.28), len: 77 aa. rpmC, 50S ribosomal protein L29, equivalent to O06050|RL29_MYCBO|MBS10OPER_10 50S ribosomal protein L29 from Mycobacterium bovis BCG (75 aa); and O32989|RL29_MYCLE|MLCB2492_10 50S ribosomal protein L29 from Mycobacterium leprae (80 aa). Also highly similar to others e.g. Q9L0D2|RL29_STRCO 50S ribosomal protein L29 from Streptomyces coelicolor (74 aa); P12873|RL29_BACSU 50s ribosomal protein l29 from Bacillus subtilis (66 aa), FASTA scores: opt: 225, E(): 8.3e-11, (58.6% identity in 58 aa overlap); etc. Belongs to the L29P family of ribosomal proteins.; 50S ribosomal protein L29 RpmC 805759 rpmC 888374 rpmC Mycobacterium tuberculosis H37Rv 50S ribosomal protein L29 RpmC NP_215223.1 805526 D 83332 CDS NP_215224.1 15607850 888391 805756..806166 1 NC_000962.3 Rv0710, (MTCY210.29), len: 136 aa. rpsQ, 30S ribosomal protein S17, equivalent to O06051|RS17_MYCBO 30S|MBS10OPER_11 30S ribosomal protein S17 from Mycobacterium bovis BCG (136 aa); and MLCB2492_11 30S ribosomal protein S17 from Mycobacterium leprae (126 aa). Also highly similar to others e.g. CAB82079.1|AL161803 30S ribosomal protein S17 from Streptomyces coelicolor (95 aa); P12874|RS17_BACSU 30s ribosomal protein s17 (bs 16) from Bacillus subtilis (86 aa), FASTA scores: opt: 305, E(): 1.6e-11, (60.5% identity in 81 aa overlap); etc. Contains PS00056 Ribosomal protein S17 signature.; 30S ribosomal protein S17 RpsQ 806166 rpsQ 888391 rpsQ Mycobacterium tuberculosis H37Rv 30S ribosomal protein S17 RpsQ NP_215224.1 805756 D 83332 CDS NP_215225.1 15607851 888394 806335..808698 1 NC_000962.3 Rv0711, (MTCY210.30), len: 787 aa. Possible atsA,arylsulfatase, similar to others e.g. P51691|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 439, E(): 2.9e-21, (30.8% identity in 552 aa overlap); etc. Also similar to other hypothetical arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c, Rv0663, etc. Contains PS00523 Sulfatases signature 1, and PS00149 Sulfatases signature 2. Belongs to the sulfatase family.; Possible arylsulfatase AtsA (aryl-sulfate sulphohydrolase) (arylsulphatase) 808698 atsA 888394 atsA Mycobacterium tuberculosis H37Rv Possible arylsulfatase AtsA (aryl-sulfate sulphohydrolase) (arylsulphatase) NP_215225.1 806335 D 83332 CDS NP_215226.1 15607852 888346 808746..809645 1 NC_000962.3 Rv0712, (MTCY210.31), len: 299 aa. Conserved protein, similar to others e.g. NP_106128.1|NC_002678 hypothetical protein from Mesorhizobium loti (372 aa); D90901_33|P72841 hypothetical 48.1 kDa protein from Synechocystis sp (410 aa), FASTA scores: E(): 1.1e-07,(28.8% identity in 299 aa overlap); etc. Slight similarity to carboxykinases. Similar to C-terminal part of Rv3703c conserved hypothetical protein from Mycobacterium tuberculosis (425 aa).; hypothetical protein 809645 888346 Rv0712 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215226.1 808746 D 83332 CDS NP_215227.1 15607853 888405 809946..810887 1 NC_000962.3 Rv0713, (MTCY210.32), len: 313 aa. Probable conserved transmembrane protein, similar to Rv3435c|MTCY77_7|O06252 from Mycobacterium tuberculosis (284 aa), FASTA scores: opt: 557, E(): 2.1e-29, (35.8% identity in 282 aa overlap); MLCB2492_12|O32991 hypothetical 10.7 kDa protein from Mycobacterium leprae (95 aa).; Probable conserved transmembrane protein 810887 888405 Rv0713 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215227.1 809946 D 83332 CDS NP_215228.1 15607854 888411 811373..811741 1 NC_000962.3 Rv0714, (MTCY210.33), len: 122 aa. rplN, 50S ribosomal protein L14, equivalent to O32993|MLCB2492_14|ML1849|RL14_MYCLE 50S ribosomal protein L14 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82080.1|AL161803 50S ribosomal protein L14 from Streptomyces coelicolor (122 aa); P33100|RL14_MICLU 50s ribosomal protein L14 from Micrococcus luteus (122 aa), FASTA scores: opt: 674, E(): 0, (85.2% identity in 122 aa overlap); etc. Contains PS00049 Ribosomal protein L14 signature. Belongs to the L14P family of ribosomal proteins.; 50S ribosomal protein L14 RplN 811741 rplN 888411 rplN Mycobacterium tuberculosis H37Rv 50S ribosomal protein L14 RplN NP_215228.1 811373 D 83332 CDS NP_215229.1 15607855 888421 811742..812059 1 NC_000962.3 Rv0715, (MTCY210.34), len: 105 aa. rplX, 50S ribosomal protein L24, equivalent to O32994|MLCB2492_15 50S ribosomal protein L24 from Mycobacterium leprae (105 aa). Also highly similar to others e.g. CAB82081.1|AL161803 50S ribosomal protein L24 from Streptomyces coelicolor (107 aa); P12876|RL24_BACSU 50s ribosomal protein L24 (bl23) from Bacillus subtilis (103 aa), FASTA scores: opt: 363,E(): 1.8e-18, (56.7% identity in 104 aa overlap); etc. Contains PS01108 Ribosomal protein L24 signature. Belongs to the L24P family of ribosomal proteins.; 50S ribosomal protein L24 RplX 812059 rplX 888421 rplX Mycobacterium tuberculosis H37Rv 50S ribosomal protein L24 RplX NP_215229.1 811742 D 83332 CDS NP_215230.1 15607856 888400 812059..812622 1 NC_000962.3 Rv0716, (MTCY210.35), len: 187 aa. rplE, 50S ribosomal protein L5, equivalent to MLCB2492_16 50S ribosomal protein L5 from Mycobacterium leprae (187 aa). Also highly similar to others e.g. CAB82082.1|AL161803 50S ribosomal protein L5 from Streptomyces coelicolor (185 aa); P33098|RL5_MICLU 50S ribosomal protein L5 from Micrococcus luteus (191 aa), FASTA scores: opt: 930, E(): 0, (73.8% identity in 183 aa overlap); etc. Belongs to the L5P family of ribosomal proteins.; 50S ribosomal protein L5 RplE 812622 rplE 888400 rplE Mycobacterium tuberculosis H37Rv 50S ribosomal protein L5 RplE NP_215230.1 812059 D 83332 CDS YP_177747.1 57116769 888414 812627..812812 1 NC_000962.3 Rv0717, (MTCY210.36), len: 61 aa. rpsN1, 30S ribosomal protein S14, equivalent to MLCB2492_17|O32996 ribosomal protein S14 from Mycobacterium leprae (61 aa). Also highly similar to others e.g. CAB82083.1|AL161803 30S ribosomal protein S14 from Streptomyces coelicolor (61 aa); P24320|RS14_THETH 30s ribosomal protein S14 from Thermus aquaticus (subsp. thermophilus) (60 aa), FASTA scores: opt: 316, E(): 2e-19,(70.0% identity in 60 aa overlap); etc. Contains PS00527 Ribosomal protein S14 signature. Belongs to the S14P family of ribosomal proteins. Note that previously known as rpsN.; 30S ribosomal protein S14 RpsN1 812812 rpsN1 888414 rpsN1 Mycobacterium tuberculosis H37Rv 30S ribosomal protein S14 RpsN1 YP_177747.1 812627 D 83332 CDS NP_215232.1 15607858 888424 812976..813374 1 NC_000962.3 Rv0718, (MTCY210.37), len: 132 aa. rpsH, 30S ribosomal protein S8, equivalent to O32997|MLCB2492_18 30S ribosomal protein S8 from Mycobacterium leprae (132 aa). Also highly similar to others e.g. CAB82084.1|AL161803 30S ribosomal protein S8 from Streptomyces coelicolor (132 aa); P33106|RS8_MICLU 30s ribosomal protein S8 from Micrococcus luteus (132 aa), FASTA scores: opt: 669, E(): 0, (77.3% identity in 132 aa overlap); etc. Contains PS00053 Ribosomal protein S8 signature. Belongs to the S8P family of ribosomal proteins.; 30S ribosomal protein S8 RpsH 813374 rpsH 888424 rpsH Mycobacterium tuberculosis H37Rv 30S ribosomal protein S8 RpsH NP_215232.1 812976 D 83332 CDS NP_215233.1 15607859 888433 813398..813937 1 NC_000962.3 Rv0719, (MTCY210.38), len: 179 aa. rplF, 50S ribosomal protein L6, equivalent to O32998|MLCB2492_19 50S ribosomal protein L6 from Mycobacterium leprae (179 aa). Also highly similar to others e.g. P46786|RL6_STRCO|CAB82085.1|AL161803|SCD31.42 50S ribosomal protein L6 from Streptomyces coelicolor (179 aa), FASTA scores: opt: 872, E(): 0, (70.4% identity in 179 aa overlap); etc. Contains PS00525 Ribosomal protein L6 signature 1. Belongs to the L6P family of ribosomal proteins.; 50S ribosomal protein L6 RplF 813937 rplF 888433 rplF Mycobacterium tuberculosis H37Rv 50S ribosomal protein L6 RplF NP_215233.1 813398 D 83332 CDS NP_215234.1 15607860 888457 813940..814308 1 NC_000962.3 Rv0720, (MTCY210.39), len: 122 aa. rplR, 50S ribosomal protein L18, equivalent to O32999|MLCB2492_20|RL18_MYCLE 50S ribosomal protein L18 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82086.1|AL161803 50S ribosomal protein L18 from Streptomyces coelicolor (127 aa); P33102|RL18_MICLU 50s ribosomal protein L18 from Micrococcus luteus (119 aa),FASTA scores: opt: 447, E(): 8.7e-24, (60.4% identity in 111 aa overlap); etc. Belongs to the L18P family of ribosomal proteins.; 50S ribosomal protein L18 RplR 814308 rplR 888457 rplR Mycobacterium tuberculosis H37Rv 50S ribosomal protein L18 RplR NP_215234.1 813940 D 83332 CDS NP_215235.1 15607861 888465 814328..814990 1 NC_000962.3 Rv0721, (MTCY210.40), len: 220 aa. rpsE, 30S ribosomal protein S5, equivalent to MLCB2492_21 ribosomal protein S5 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. P46790|RS5_STRCO 30s ribosomal protein S5 from Streptomyces coelicolor (167 aa), FASTA scores: opt: 889, E(): 0, (82.1% identity in 162 aa overlap); etc. Note N-terminus is extented compared to other rpsE genes. Contains PS00585 Ribosomal protein S5 signature, PTS HPr component phosphorylation sites signature. Belongs to the S5P family of ribosomal proteins.; 30S ribosomal protein S5 RpsE 814990 rpsE 888465 rpsE Mycobacterium tuberculosis H37Rv 30S ribosomal protein S5 RpsE NP_215235.1 814328 D 83332 CDS NP_215236.1 15607862 888505 814993..815190 1 NC_000962.3 Rv0722, (MTCY210.41), len: 65 aa. rpmD, 50S ribosomal protein L30, equivalent to O33001 ribosomal protein L30 from Mycobacterium leprae (71 aa). Also highly similar to others e.g. P46789|RL30_STRCO 50S ribosomal protein L30 from Streptomyces coelicolor (60 aa); P02430|RL30_ECOLI 50S ribosomal protein L30 from Escherichia coli (58 aa), FASTA scores: opt: 168, E(): 1.5e-13, (53.7% identity in 54 aa overlap); etc. Belongs to the L30P family of ribosomal proteins.; 50S ribosomal protein L30 RpmD 815190 rpmD 888505 rpmD Mycobacterium tuberculosis H37Rv 50S ribosomal protein L30 RpmD NP_215236.1 814993 D 83332 CDS NP_215237.1 15607863 888531 815190..815630 1 NC_000962.3 Rv0723, (MTCY210.42), len: 146 aa. rplO, 50S ribosomal protein L15, equivalent to MLCB2492_23|O33002 50S ribosomal protein L15 from Mycobacterium leprae (146 aa). Also highly similar to others e.g. P46787|RL15_STRCO|SCD31.46 50S ribosomal protein L15 from Streptomyces coelicolor (151 aa); P19946|RL15_BACSU 50s ribosomal protein L15 from Bacillus subtilis (146 aa),FASTA scores: opt: 419, E(): 6.5e-20, (51.0% identity in 145 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00475 Ribosomal protein L15 signature. Belongs to the L15P family of ribosomal proteins.; 50S ribosomal protein L15 RplO 815630 rplO 888531 rplO Mycobacterium tuberculosis H37Rv 50S ribosomal protein L15 RplO NP_215237.1 815190 D 83332 CDS NP_215238.1 15607864 888535 815663..817534 1 NC_000962.3 Rv0724, (MTCY210.43), len: 623 aa. Possible sppA,protease IV (endopeptidase IV), equivalent (but longer 23 aa) to MLCB2492_24|O33003 endopeptidase IV from Mycobacterium leprae (602 aa). Also similar to others e.g. NP_419743.1|NC_002696 signal peptide peptidase SppA from Caulobacter crescentus (594 aa); P08395|SPPA_ECOLI|B1766 protease IV (endopeptidase) from Escherichia coli strain K-12 (618 aa), FASTA scores: opt: 582, E(): 8.9e-27, (34.1% identity in 525 aa overlap); etc. Belongs to peptidase family S49. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Possible protease IV SppA (endopeptidase IV) (signal peptide peptidase) 817534 sppA 888535 sppA Mycobacterium tuberculosis H37Rv Possible protease IV SppA (endopeptidase IV) (signal peptide peptidase) NP_215238.1 815663 D 83332 CDS YP_177631.1 57116770 3205058 complement(817531..>817866) 1 NC_000962.3 Rv0724A, len: 111 aa. Similarity suggests that this CDS should be continuation of Rv0725c but we can find no frame-shift to account for this. Possible extended protein is very similar to other hypothetical Mycobacterium tuberculosis proteins e.g. Rv1729c|Z81360_12 (312 aa),FASTA scores: opt: 399, E(): 2e-19, (58.7% identity in 109 aa overlap); Rv0731c, Rv0726c, etc. Frame-shift could occur at nt 817866. Same sequence for strain CDC1551 and Mycobacterium bovis.; hypothetical protein >817866 3205058 Rv0724A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177631.1 817531 R 83332 CDS NP_215239.1 15607865 888447 complement(817539..818444) 1 NC_000962.3 Rv0725c, (MTCY210.44c), len: 301 aa. Conserved hypothetical protein, similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0726c, Rv0731c, Rv3399,etc, e.g. Y893_MYCTU|Q10552|Rv0893C hypothetical 36.1 kDa protein cy31.21c (325 aa), FASTA scores: opt: 600, E(): 3.9e-32, (43.8% identity in 219 aa overlap).; hypothetical protein 818444 888447 Rv0725c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215239.1 817539 R 83332 CDS NP_215240.1 15607866 888552 complement(818537..819640) 1 NC_000962.3 Rv0726c, (MTCY210.45c), len: 367 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Q10552|Y893_MYCTU|Rv0893c|MT0917|MTCY31.21c (325 aa), FASTA scores: opt: 646, E(): 0, (38.3% identity in 329 aa overlap); Rv0731c|MTV041.05c (318 aa), Rv3399, etc. Also similar to proteins from Mycobacterium leprae and other organisms e.g. T35930 hypothetical protein SC9B5.10 from Streptomyces coelicolor (303 aa).; Possible S-adenosylmethionine-dependent methyltransferase 819640 888552 Rv0726c Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_215240.1 818537 R 83332 CDS NP_215241.1 15607867 888550 complement(819843..820499) 1 NC_000962.3 Rv0727c, (MTV41.01c, MTCY210.46c), len: 218 aa. Possible fucA, L-fuculose-1-phosphate aldolase, similar to many e.g. NP_386339.1|NC_003047 putative L-fuculose phosphate aldolase protein from Sinorhizobium meliloti (222 aa); P11550|FUCA_ECOLI L-fuculose phosphate aldolase from Escherichia strain K12 (215 aa), FASTA scores: opt: 372,E(): 4.1e-19, (34.6% identity in 185 aa overlap); etc. Belongs to the aldolase class II family, ARAD/FUCA subfamily. Cofactor: binds one zinc ion per molecule.; Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase) 820499 fucA 888550 fucA Mycobacterium tuberculosis H37Rv Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase) NP_215241.1 819843 R 83332 CDS NP_215242.1 15607868 888555 complement(820496..821476) 1 NC_000962.3 Rv0728c, (MTV041.02c), len: 326 aa. Possible serA2,D-3-phosphoglycerate dehydrogenase, similar to others e.g. AF0278|AF027868_5|YoaD D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (344 aa), FASTA scores: opt: 594,E(): 3.1e-31, (35.9% identity in 309 aa overlap); etc. Also similar to Rv2996c|MTV012.10|SERA1 D-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis (528 aa).; Possible D-3-phosphoglycerate dehydrogenase SerA2 (phosphoglycerate dehydrogenase) (PGDH) 821476 serA2 888555 serA2 Mycobacterium tuberculosis H37Rv Possible D-3-phosphoglycerate dehydrogenase SerA2 (phosphoglycerate dehydrogenase) (PGDH) NP_215242.1 820496 R 83332 CDS NP_215243.1 15607869 888548 821507..822853 1 NC_000962.3 Rv0729, (MTV041.03), len: 448 aa. Possible xylB,D-xylulose-kinase (xylulokinase). C-terminus highly similar to AAD09880.1|U77912 unknown protein from Mycobacterium bovis (102 aa); and N-terminus highly similar to T45387|Z98756|MLCB2492_25 hypothetical protein from Mycobacterium leprae (110 aa), FASTA scores: opt: 427, E(): 1.1e-19, (60.9% identity in 110 aa overlap). Also similar to xylA/xylB genes from various bacterial species e.g. AAC26499.1|AF045245 D-xylulose-kinase from Klebsiella pneumoniae (487 aa); NP_418021.1|NC_000913 xylulokinase from Escherichia coli strain K12 (484 aa), FASTA scores: opt: 260, E(): 7.5e-09, (25.9% identity in 478 aa overlap); etc. Also similar to Rv3696c|glpK probable glycerol kinase from Mycobacterium tuberculosis (517 aa). Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family.; Possible D-xylulose kinase XylB (xylulokinase) (xylulose kinase) 822853 xylB 888548 xylB Mycobacterium tuberculosis H37Rv Possible D-xylulose kinase XylB (xylulokinase) (xylulose kinase) NP_215243.1 821507 D 83332 CDS NP_215244.1 15607870 888558 822866..823594 1 NC_000962.3 Rv0730, (MTV041.04), len: 242 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to Z98756|MLCB2492_26 hypothetical protein from Mycobacterium leprae (227 aa), FASTA scores: opt: 1180, E(): 0, (83.5% identity in 218 aa overlap).; GCN5-related N-acetyltransferase 823594 888558 Rv0730 Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_215244.1 822866 D 83332 CDS NP_215245.1 15607871 888556 complement(823683..824639) 1 NC_000962.3 Rv0731c, (MTV041.05c), len: 318 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Rv0726c|MTCY210.45c (367 aa), FASTA score: (60.9% identity in 317 aa overlap); Rv3399, Rv1729c, etc.; Possible S-adenosylmethionine-dependent methyltransferase 824639 888556 Rv0731c Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_215245.1 823683 R 83332 CDS NP_215246.1 15607872 888559 824800..826125 1 NC_000962.3 Rv0732, (MTV041.06), len: 441 aa. Probable SecY,preprotein translocase (integral membrane protein) (see citation below), equivalent to NP_302243.1|NC_002677 SecY subunit of preprotein translocase from Mycobacterium leprae (438 aa); AAC04389.1|AF047021 preprotein translocase subunit from Mycobacterium smegmatis (438 aa); and U77912|MBU77912_1 preprotein translocase subunit from Mycobacterium bovis (441 aa), FASTA scores: opt: 2802, E(): 0, (99.8% identity in 441 aa overlap). Also highly similar to others e.g. P46785|SECY_STRCO preprotein translocase SECY subunit from Streptomyces coelicolor (437 aa); etc. Contains PS00755 and PS00756 protein secY signatures 1 and 2. Belongs to the SECE/SEC61-alpha family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c,SECG|Rv1440 and SECY.; Probable preprotein translocase SecY 826125 secY 888559 secY Mycobacterium tuberculosis H37Rv Probable preprotein translocase SecY NP_215246.1 824800 D 83332 CDS NP_215247.1 15607873 888567 826122..826667 1 NC_000962.3 Rv0733, (MTV041.07), len: 181 aa. adk, adenylate kinase (ATP-AMP transphosphorylase), equivalent to Z98756|MLCB24 92_28 probable adenylate kinase from Mycobacterium leprae (181 aa), FASTA scores: opt: 978, E(): 0, (83.6% identity in 177 aa overlap); and AAF86323.1|AF271342 putative adenylate kinase from Mycobacterium marinum (124 aa) (N-terminus shorter). Also highly similar to others e.g. P43414|KAD_STRCO adenylate kinase from Streptomyces coelicolor (217 aa), FASTA score: (43.0% identity in 186 aa overlap); etc. Contains PS00113 Adenylate kinase signature. Belongs to the adenylate kinase family.; Adenylate kinase Adk (ATP-AMP transphosphorylase) 826667 adk 888567 adk Mycobacterium tuberculosis H37Rv Adenylate kinase Adk (ATP-AMP transphosphorylase) NP_215247.1 826122 D 83332 CDS YP_177748.1 57116771 888564 826670..827470 1 NC_000962.3 Rv0734, (MTV041.08), len: 266 aa. mapA, methionine aminopeptidase (map), equivalent to Z98756|MLCB2492_29 probable methionine aminopeptidase from Mycobacterium leprae (266 aa), FASTA scores: opt: 1717, E(): 0, (83.4% identity in 265 aa overlap). Also highly similar to many e.g. T35553 methionine aminopeptidase from Streptomyces coelicolor (278 aa); etc. Also similar to Rv2861c|MAPB probable methionine aminopeptidase from Mycobacterium tuberculosis (285 aa). Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Conserved in M. tuberculosis, M. leprae,M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Methionine aminopeptidase MapA (map) (peptidase M) (MetAP) 827470 mapA 888564 mapA Mycobacterium tuberculosis H37Rv Methionine aminopeptidase MapA (map) (peptidase M) (MetAP) YP_177748.1 826670 D 83332 CDS NP_215249.1 15607875 888609 827543..828076 1 NC_000962.3 Rv0735, (MTV041.09), len: 177 aa. Probable sigL,alternative RNA polymerase sigma factor (rpoE) (see citations below), highly similar to many proteins of the extracytoplasmatic function (ECF) subfamily e.g. CAB72200.1|AL138851 putative RNA polymerase sigma factor from Streptomyces coelicolor (194 aa); Q06909|CARQ_MYXXA RNA polymerase sigma factor CARQ from Myxococcus xanthus (174 aa), FASTA scores: opt: 251, E(): 9.6e-11, (32.9% identity in 161 aa overlap); etc. Also similar to MTCI61_4,MTU87242_1, and MLU15180_30 from Mycobacterium tuberculosis. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 139-160 (Score 1134, +3.05 SD). Belongs to the sigma-70 factor family, ECF subfamily.; Probable alternative RNA polymerase sigma factor SigL 828076 sigL 888609 sigL Mycobacterium tuberculosis H37Rv Probable alternative RNA polymerase sigma factor SigL NP_215249.1 827543 D 83332 CDS NP_215250.1 15607876 888611 828140..828892 1 NC_000962.3 Rv0736, (MTV041.10), len: 250 aa. RslA, anti-sigma factor (See Dainese et al., 2006). Probable membrane protein, showing weak similarity with AL133469|SCM10_32 putative membrane protein from Streptomyces coelicolor (216 aa), FASTA scores: opt: 180, E(): 0.00018, (34.3% identity in 216 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010).; Anti-sigma factor RslA 828892 rslA 888611 rslA Mycobacterium tuberculosis H37Rv Anti-sigma factor RslA NP_215250.1 828140 D 83332 CDS NP_215251.1 15607877 888619 829207..829704 1 NC_000962.3 Rv0737, (MTV041.11), len: 165 aa. Possible transcriptional regulator, similar to others e.g. BAB69161.1|AB070937 regulator protein from Streptomyces avermitilis (169 aa); NP_419731.1|NC_002696 transcriptional regulator MarR family from Caulobacter crescentus (148 aa) (homology only at C-terminus); etc. Also shows weak similarity to AB0014|AB001488_14 hypothetical protein from Bacillus subtilis (164 aa), FASTA scores: opt: 163, E(): 9.3e-05, (32.8% identity in 116 aa overlap), which is similar to slyY gene of S. typhimurium required for survival in macrophage. Contains possible helix-turn helix motif from aa 73-94 (Score 1138, +3.06 SD).; Possible transcriptional regulatory protein 829704 888619 Rv0737 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_215251.1 829207 D 83332 CDS NP_215252.1 15607878 888620 830062..830610 1 NC_000962.3 Rv0738, (MTV041.12), len: 182 aa. Conserved protein,showing weak similarity with hypothetical proteins from Mycobacterium tuberculosis: Rv1727|MTCY04C12.12 (189 aa); MTY13D12_7|Z80343 hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: opt: 172, E(): 0.0004,(24.2% identity in 178 aa overlap); and C-terminus of Rv0576.; hypothetical protein 830610 888620 Rv0738 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215252.1 830062 D 83332 CDS NP_215253.1 15607879 888622 830855..831661 1 NC_000962.3 Rv0739, (MTV041.13), len: 268 aa. Conserved hypothetical protein, showing some similarity to Mycobacterium tuberculosis proteins Rv0026 (448 aa), FASTA score: (37.6% identity in 101 aa overlap)and Rv0025 (120 aa), FASTA score: (32.4% identity in 142 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 831661 888622 Rv0739 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215253.1 830855 D 83332 CDS NP_215254.1 15607880 888638 831776..832303 1 NC_000962.3 Rv0740, (MTV041.14), len: 175 aa. Conserved hypothetical protein; C-terminus (possibly part of truncated IS1557) shows nearly perfect identity to Rv0750|MTV041_24 (81 aa), FASTA score: (92.6% identity in 81 aa overlap). Also shows weak similarity to MTV007_5 hypothetical protein from Mycobacterium tuberculosis (313 aa), FASTA score: (34.5% identity in 110 aa overlap); and MLCL536_27 hypothetical protein from Mycobacterium leprae (315 aa), FASTA score: (34.5% identity in 84 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 832303 888638 Rv0740 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215254.1 831776 D 83332 CDS NP_215255.1 15607881 888644 832534..832848 1 NC_000962.3 Rv0741, (MTV041.15), len: 104 aa. Probable truncated transposase for IS1557, showing similarity to transposases and is elements e.g. U63997|EFU63997_1 insertion sequence from Enterococcus faecium (424 aa), FASTA score: (31.0% identity in 87 aa overlap). Very high similarity with the C-terminal part of Z73419|MTCY373_3 2 IS1557 from Mycobacterium tuberculosis (444 aa), FASTA score: (86.5% identity in 104 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable transposase (fragment) 832848 888644 Rv0741 Mycobacterium tuberculosis H37Rv Probable transposase (fragment) NP_215255.1 832534 D 83332 CDS YP_177749.1 57116772 888645 832981..833508 1 NC_000962.3 Rv0742, (MTV041.16), len: 175 aa. PE_PGRS8, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many Mycobacterium tuberculosis PGRS-type proteins e.g. Z78020|MTCY1A11_25 (498 aa), FASTA scores: opt: 766, E(): 6.1e-25, (73.6% identity in 178 aa overlap). Similarity suggests ORF starts with ATA start codon. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE-PGRS family protein PE_PGRS8 833508 PE_PGRS8 888645 PE_PGRS8 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS8 YP_177749.1 832981 D 83332 CDS NP_215257.1 15607883 888641 complement(833886..834443) 1 NC_000962.3 Rv0743c, (MTV041.17c), len: 185 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 834443 888641 Rv0743c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215257.1 833886 R 83332 CDS NP_215258.1 15607884 888648 complement(834440..834946) 1 NC_000962.3 Rv0744c, (MTV041.18c), len: 168 aa. Possible transcriptional regulator, showing weak similarity with O86661|SC4A2.05 putative two-component sensor from Streptomyces coelicolor (436 aa), FASTA scores: opt: 117,E(): 0.88, (37.25% identity in 94 aa overlap); and some putative excisionases or transposases. Also weakly similar to P71902|YN10_MYCTU|Rv2310|MT2372|MTCY3G12.24c conserved hypothetical protein from Mycobacterium tuberculosis (114 aa); and Q11144|Y477_MYCTU|Rv0477|MT0495|MTCY20G9.03 conserved hypothetical protein from Mycobacterium tuberculosis (148 aa). Equivalent to AAK45006 from Mycobacterium tuberculosis strain CDC1551 (179 aa) but shorter 11 aa. Contains probable helix-turn helix motif from aa 5-26 (Score 1350, +3.78 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible transcriptional regulatory protein 834946 888648 Rv0744c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_215258.1 834440 R 83332 CDS NP_215259.1 15607885 888595 835154..835681 1 NC_000962.3 Rv0745, (MTV041.19), len: 175 aa. Conserved hypothetical protein; shows high similarity to a 50 aa region of Rv3649|Z95436|MTY15C10_3 conserved hypothetical protein, similar to ATP-dependent helicases, from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 225, E(): 7e-06, (70.0% identity in 50 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 835681 888595 Rv0745 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215259.1 835154 D 83332 CDS YP_177750.1 57116773 888664 835701..838052 1 NC_000962.3 Rv0746, (MTV041.20), len: 783 aa. PE_PGRS9, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2429, E(): 0,(56.9% identity in 873 aa overlap). Also similar to other PE-PGRS family proteins e.g. AL0212|MTV008_46 FASTA score: (48.8% identity in 887 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE-PGRS family protein PE_PGRS9 838052 PE_PGRS9 888664 PE_PGRS9 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS9 YP_177750.1 835701 D 83332 CDS YP_177751.1 57116774 888662 838451..840856 1 NC_000962.3 Rv0747, (MTV041.21), len: 801 aa. PE_PGRS10, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2772, E(): 0,(60.9% identity in 941 aa overlap). Also similar to other PE-PGRS family proteins e.g. Z95844|MTCY493_2 FASTA score: (50.2% identity in 815 aa overlap). Contains PS00012 Phosphopantetheine attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE-PGRS family protein PE_PGRS10 840856 PE_PGRS10 888662 PE_PGRS10 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS10 YP_177751.1 838451 D 83332 CDS NP_215262.1 15607888 888682 840947..841204 1 NC_000962.3 Rv0748, (MTV041.22), len: 85 aa. Possible vapB31,antitoxin, part of toxin-antitoxin (TA) operon with Rv0749,see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis proteins e.g. Rv2871 (75 aa); Rv1241, Rv2132, Rv3321c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible antitoxin VapB31 841204 vapB31 888682 vapB31 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB31 NP_215262.1 840947 D 83332 CDS NP_215263.1 15607889 888681 841228..841656 1 NC_000962.3 Rv0749, (MTV041.23), len: 142 aa. Possible vapC31,toxin, part of toxin-antitoxin (TA) operon with Rv0748,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv0277c, Rv2530c,etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC31 Contains PIN domain 841656 vapC31 888681 vapC31 Mycobacterium tuberculosis H37Rv Possible toxin VapC31 Contains PIN domain NP_215263.1 841228 D 83332 CDS YP_177632.1 57116775 3205052 complement(841737..841874) 1 NC_000962.3 Rv0749A, len: 45 aa. Conserved hypothetical protein (probably gene fragment), similar to part (aa 250-292) of Rv2807|Z81331_12 from Mycobacterium tuberculosis (384 aa),FASTA scores: opt: 238, E(): 1.9e-13, (79.07% identity in 43 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 841874 3205052 Rv0749A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177632.1 841737 R 83332 CDS NP_215264.1 15607890 888688 842033..842278 1 NC_000962.3 Rv0750, (MTV041.24), len: 81 aa. Conserved hypothetical protein, showing almost perfect overlap with C-terminus of Rv0740|MTV041_14 conserved hypothetical protein from Mycobacterium tuberculosis (175 aa), FASTA scores: (93.8% identity in 81 aa overlap). Possible duplication. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 842278 888688 Rv0750 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215264.1 842033 D 83332 CDS NP_215265.1 15607891 888658 complement(842347..843231) 1 NC_000962.3 Rv0751c, (MTV041.25c), len: 294 aa. Probable mmsB,3-hydroxyisobutyrate dehydrogenase, highly similar to others e.g. NP_102847.1|NC_002678 3-hydroxyisobutyrate dehydrogenase from Mesorhizobium loti (294 aa); NP_420167.1|NC_002696 3-hydroxyisobutyrate dehydrogenase from Caulobacter crescentus (298 aa); A32867 3-hydroxyisobutyrate dehydrogenase from Rattus norvegicus (346 aa); etc. Also similar to methylmalonate semialdehyde dehydrogenases e.g. M84911|PSE MMSRAB_3 methylmalonate semialdehyde dehydrogenase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 786, E(): 0, (45.8% identity in 297 aa overlap). Also similar to 6-phosphogluconate dehydrogenases from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Contains PS00895 3-hydroxyisobutyrate dehydrogenase signature. Belongs to the 3-hydroxyisobutyrate dehydrogenase family.; Probable 3-hydroxyisobutyrate dehydrogenase MmsB (hibadh) 843231 mmsB 888658 mmsB Mycobacterium tuberculosis H37Rv Probable 3-hydroxyisobutyrate dehydrogenase MmsB (hibadh) NP_215265.1 842347 R 83332 CDS NP_215266.1 15607892 888684 complement(843242..844414) 1 NC_000962.3 Rv0752c, (MTV041.26c), len: 390 aa. Probable fadE9,acyl-CoA dehydrogenase, highly similar to many e.g. NP_437985.1|NC_003078 putative acyl-CoA dehydrogenase protein from Sinorhizobium meliloti (380 aa); Z99123|BSUB0020_14 from Bacillus subtilis (379 aa), FASTA scores: opt: 853, E(): 0, (39.8% identity in 384 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, and PS00073 Acyl-Co Adehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE9 844414 fadE9 888684 fadE9 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE9 NP_215266.1 843242 R 83332 CDS NP_215267.1 15607893 888707 complement(844421..845953) 1 NC_000962.3 Rv0753c, (MTV041.27c), len: 510 aa. Probable mmsA,methylmalonic acid semialdehyde dehydrogenase, highly similar to others e.g. NP_420115.1|NC_002696 putative methylmalonate-semialdehyde dehydrogenase from Caulobacter crescentus (499 aa); L48550|STMMSDA_1|CAB75315.1|AL139164 methylmalonic acid semialdehyde dehydrogenase from Streptomyces coelicolor (500 aa), FASTA score: (51.6% identity in 498 aa overlap); M84911|PSEMMSRAB_2|NP_252260.1|NC_002516 methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1127, E(): 0,(47.9% identity in 507 aa overlap); etc. Note that also highly similar to malonic semialdehyde oxidative decarboxylases e.g. NP_104968.1|NC_002678 malonic semialdehyde oxidative decarboxylase from Mesorhizobium loti (498 aa); NP_384832.1|NC_003047 putative malonic semialdehyde oxidative decarboxylase protein from Sinorhizobium meliloti (498 aa); etc. Contains PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family.; Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH) 845953 mmsA 888707 mmsA Mycobacterium tuberculosis H37Rv Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH) NP_215267.1 844421 R 83332 CDS YP_177752.1 57116776 888695 846159..847913 1 NC_000962.3 Rv0754, (MTV041.28), len: 584 aa. PE_PGRS11, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. AL0212|MTV008_46 from Mycobacterium tuberculosis (1660 aa), FASTA score: (48.7% identity in 345 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (41.6% identity in 442 aa overlap); etc.; PE-PGRS family protein PE_PGRS11 847913 PE_PGRS11 888695 PE_PGRS11 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS11 YP_177752.1 846159 D 83332 CDS YP_177753.1 57116777 888708 complement(848103..850040) 1 NC_000962.3 Rv0755c, (MTV041.29), len: 645 aa. PPE12, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. Z82098|MTCY3C7_23 from Mycobacterium tuberculosis (582 aa), FASTA scores: (56.1% identity in 636 aa overlap); Z92774|MTCY6G11_5 from Mycobacterium tuberculosis (552 aa), FASTA scores: (55.8% identity in 590 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE12 850040 PPE12 888708 PPE12 Mycobacterium tuberculosis H37Rv PPE family protein PPE12 YP_177753.1 848103 R 83332 CDS YP_177633.1 57116778 3205072 complement(850342..850527) 1 NC_000962.3 Rv0755A, len: 61 aa. Putative transposase (possibly gene fragment), similar to C-terminal part of Q9EZM2|ISMav2|AF286339_1 putative transposase from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 284, E(): 5e-13, (83.02% identity in 53 aa overlap); and to SCJ11.25c|Q9RI80 possible noncomposite transposon transposase from Streptomyces coelicolor (283 aa).; Putative transposase (fragment) 850527 3205072 Rv0755A Mycobacterium tuberculosis H37Rv Putative transposase (fragment) YP_177633.1 850342 R 83332 CDS NP_215270.1 15607896 888730 complement(850741..851466) 1 NC_000962.3 Rv0756c, (MTCY369.01c), len: 241 aa. Unknown protein.; hypothetical protein 851466 888730 Rv0756c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215270.1 850741 R 83332 CDS NP_215271.1 15607897 888772 851608..852351 1 NC_000962.3 Rv0757, (MTCY369.02), len: 247 aa. Possible phoP,two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c|MTCY31.31c|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1.; Possible two component system response transcriptional positive regulator PhoP 852351 phoP 888772 phoP Mycobacterium tuberculosis H37Rv Possible two component system response transcriptional positive regulator PhoP NP_215271.1 851608 D 83332 CDS NP_215272.1 15607898 888775 852396..853853 1 NC_000962.3 Rv0758, (MTCY369.03), len: 485 aa. Possible phoR,two component system response phosphate sensor kinase membrane-associated, highly similar to various sensor kinases e.g. CAC32361.1|AL583945 putative two component system histidine kinase from Streptomyces coelicolor (524 aa); NP_349365.1|NC_003030 Membrane-associated sensory histidine kinase with HAMP domain from Clostridium acetobutylicum (482 aa); and similar to phoP proteins e.g. NP_372216.1|NC_002758 alkaline phosphatase synthesis sensor protein from Staphylococcus aureus (554 aa); P23545|PHOR_BACSU alkaline phosphatase synthesis sensor from Bacillus subtilis (579 aa), FASTA scores: opt: 515,E(): 1.9e-25, (40.0% identity in 230 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. MTCY20G9.16 FASTA scores: (34.5% identity in 264 aa overlap), MTU88959_2 (509 aa), MTCY10G2_17, etc.; Possible two component system response sensor kinase membrane associated PhoR 853853 phoR 888775 phoR Mycobacterium tuberculosis H37Rv Possible two component system response sensor kinase membrane associated PhoR NP_215272.1 852396 D 83332 CDS NP_215273.3 448824748 888776 complement(853825..854157) 1 NC_000962.3 Rv0759c, (MTCY369.04c), len: 110 aa. Conserved hypothetical protein, highly similar (but shorter 45 aa in N-terminus) to P49774|YHIT_MYCLE|ML2237|MLCB5.04c|U296A hypothetical hit-like protein from Mycobacterium leprae (155 aa), FASTA scores: opt: 766, E(): 0, (78.7% identity in 150 aa overlap). Also highly similar (but N-terminus always shorter) to hit-like proteins and protein kinase inhibitors e.g. AAF72728.1|AF265258_1|AF265258 hit-like protein from Rhodococcus sp. (141 aa); NP_212513.1|NC_001318 protein kinase C1 inhibitor (pkcI) from Borrelia burgdorferi (149 aa) ; P94252|YHIT_BORBU|BB0379 hypothetical hit-like protein from Borrelia burgdorferi (139 aa); NP_110768.1|NC_002689 hit (histidine triad) family protein from Thermoplasma volcanium (158 aa); P16436|IPK1_BOVIN protein kinase C inhibitor 1 (pkci-1) from Bos taurus (Bovine) (125 aa),FASTA scores: opt: 195, E(): 5.2e-08, (33.3% identity in 111 aa overlap); etc. Also shows similarity with Rv2613c|MTCY01A10.20A conserved hypothetical protein from Mycobacterium tuberculosis (195 aa) and Rv1262c|MTCY50.20 hypothetical hit-like protein (144 aa).; hypothetical protein 854157 888776 Rv0759c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215273.3 853825 R 83332 CDS NP_215274.1 15607900 888784 complement(854267..854686) 1 NC_000962.3 Rv0760c, (MTCY369.05), len: 139 aa. Conserved protein, similar to N-terminal part of Rv2042c conserved hypothetical protein from Mycobacterium tuberculosis (265 aa), FASTA scores: opt: 150, E(): 4.1e-05, (28.7% identity in 136 aa overlap). Belongs to the NTF2-like (nuclear transport factor 2) protein superfamiily.; hypothetical protein 854686 888784 Rv0760c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215274.1 854267 R 83332 CDS YP_177754.1 57116779 888738 complement(854699..855826) 1 NC_000962.3 Rv0761c, (MTCY369.06c), len: 375 aa. Possible adhB,zinc-containing alcohol dehydrogenase NAD-dependent,similar to others e.g. AAC15839.1|AF060871_4 hypothetical alcohol dehydrogenase from Rhodococcus rhodochrous (370 aa), FASTA scores: opt: 1234, E(): 0, (46.8% identity in 370 aa overlap); P80468|ADH2_STRCA alcohol dehydrogenase II from Struthio camelus (Ostrich) (379 aa); Q03505|ADH1_RABIT alcohol dehydrogenase alpha chain from Oryctolagus cuniculus (Rabbit) (374 aa), FASTA scores: opt: 872, E(): 0, (39.1% identity in 379 aa overlap); etc. Also similar to adhD alcohol dehydrogenase from Mycobacterium tuberculosis (368 aa). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family.; Possible zinc-containing alcohol dehydrogenase NAD dependent AdhB 855826 adhB 888738 adhB Mycobacterium tuberculosis H37Rv Possible zinc-containing alcohol dehydrogenase NAD dependent AdhB YP_177754.1 854699 R 83332 CDS NP_215276.1 15607902 888807 complement(855925..856470) 1 NC_000962.3 Rv0762c, (MTCY369.07c), len: 181 aa. Conserved hypothetical protein, showing weak similarity to D90907_77|P73575 hypothetical 31.3KD protein from Synechocystis sp, FASTA scores: E(): 0.0012, (30.4% identity in 92 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop).; hypothetical protein 856470 888807 Rv0762c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215276.1 855925 R 83332 CDS NP_215277.1 15607903 888814 complement(856473..856679) 1 NC_000962.3 Rv0763c, (MTCY369.08c), len: 68 aa. Possible ferredoxin, similar to others and related proteins e.g. P18324|FER1_STRGO|SUAB ferredoxin 1 (fd-1) from Streptomyces griseolus (68 aa); AAK31349.1|AF350429_2|AF350429 putative ferredoxin from Nocardioides sp (63 aa); AAK16536.1|AF331043_16|AF331043 phthalate dioxygenase ferredoxin subunit from Arthrobacter keyseri (64 aa); etc. Probably involved in electron transport for cytochrome P-450 system e.g. downstream ORF Rv0764c|MTCY369.09c probable cytochrome P450 51 from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 137, E(): 0.00013, (36.4% identity in 66 aa overlap). Also similar to putative ferredoxins Rv3503c and Rv1786 from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family.; Possible ferredoxin 856679 888814 Rv0763c Mycobacterium tuberculosis H37Rv Possible ferredoxin NP_215277.1 856473 R 83332 CDS NP_215278.1 15607904 888819 complement(856682..858037) 1 NC_000962.3 Rv0764c, (MT0788, MTCY369.09c), len: 451 aa. Cyp51,cytochrome P450 51 (sterol 14-alpha demethylase), similar to others e.g. Q16850|CP51_HUMAN cytochrome P450 51 (CYPL1) (P450L1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) from Homo sapiens (509 aa), FASTA scores: opt: 848, E(): 0, (33.9% identity in 439 aa overlap); NP_172633.1|NC_003070 putative obtusifoliol 14-alpha demethylase from Arabidopsis thaliana (488 aa); P93596|CP51_WHEAT cytochrome P450 51 (CYPL1) (P450-L1A1) (obtusifoliol 14-alpha demethylase) from Triticum aestivum (453 aa); etc. Also similar to many other Mycobacterium tuberculosis cytochromes P450 e.g. Rv1394c, FASTA score: (22.5% identity in 444 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Cytochrome P450 51 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM) 858037 cyp51 888819 cyp51 Mycobacterium tuberculosis H37Rv Cytochrome P450 51 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM) NP_215278.1 856682 R 83332 CDS NP_215279.1 15607905 888793 complement(858037..858864) 1 NC_000962.3 Rv0765c, (MTCY369.10c), len: 275 aa. Probable oxidoreductase, similar others e.g. P39071|DHBA_BACSU 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase from Bacillus subtilis (261 aa), FASTA scores: opt: 385, E(): 1.8e-17, (30.6% identity in 252 aa overlap); AAF81239.1|AF263012 putative beta-ketoacyl reductase from Streptomyces griseus (274 aa); NP_436514.1|NC_003037 putative oxidoreductase from Sinorhizobium meliloti (240 aa); etc. Also similar to several other oxidoreductases from Mycobacterium tuberculosis e.g. Rv1544|MTCY48.21,FASTA score: (32.6% identity in 267 aa overlap); etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature.; Probable oxidoreductase 858864 888793 Rv0765c Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_215279.1 858037 R 83332 CDS NP_215280.1 15607906 888834 complement(858864..860072) 1 NC_000962.3 Rv0766c, (MT0790, MTCY369.11c), len: 402 aa. Probable cyp123, cytochrome P-450, similar to others e.g. P33271|CPXK_SACER cytochrome P-450 107B1 from Saccharopolyspora erythraea (405 aa), FASTA scores: opt: 770, E(): 0, (36.9% identity in 406 aa overlap); T36526 probable cytochrome P450 hydroxylase from Streptomyces coelicolor (411 aa); P27632|CPXM_BACSU cytochrome P450 109 from Bacillus subtilis (405 aa); etc. Also similar to several other cytochromes P-450 from Mycobacterium tuberculosis e.g. Rv1256c|MTCY50.26 (405 aa), FASTA score: (35.2% identity in 389 aa overlap); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Probable cytochrome P450 123 Cyp123 860072 cyp123 888834 cyp123 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 123 Cyp123 NP_215280.1 858864 R 83332 CDS NP_215281.1 15607907 888833 complement(860069..860710) 1 NC_000962.3 Rv0767c, (MTCY369.12c), len: 213 aa. Conserved hypothetical protein, showing weak similarity with AL133220|SCC75A_26 hypothetical protein from Streptomyces coelicolor (215 aa), FASTA scores: opt: 152, E(): 0.0048,(28.4% identity in 204 aa overlap).; hypothetical protein 860710 888833 Rv0767c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215281.1 860069 R 83332 CDS NP_215282.1 15607908 888832 860912..862381 1 NC_000962.3 Rv0768, (MTCY369.13), len: 489 aa. Probable aldA,NAD-dependent aldehyde dehydrogenase, highly similar to others e.g. AAL14238.1|AY052630 6-oxolauric acid dehydrogenase from Rhodococcus ruber (474 aa); NP_285450.1|NC_001264 aldehyde dehydrogenase from Deinococcus radiodurans (495 aa); NP_241405.1|NC_002570 NADP-dependent aldehyde dehydrogenase from Bacillus halodurans (498 aa); P42757|DHAB_ATRHO betaine-aldehyde dehydrogenase precursor from Atriplex hortensis (Mountain spinach) (502 aa), FASTA scores: opt: 1001, E(): 0, (35.6% identity in 486 aa overlap); etc. Also highly similar to Rv0223c aldehyde dehydrogenase from Mycobacterium tuberculosis (487 aa). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family.; Probable aldehyde dehydrogenase NAD dependent AldA (aldehyde dehydrogenase [NAD+]) 862381 aldA 888832 aldA Mycobacterium tuberculosis H37Rv Probable aldehyde dehydrogenase NAD dependent AldA (aldehyde dehydrogenase [NAD+]) NP_215282.1 860912 D 83332 CDS NP_215283.1 15607909 888837 862412..863158 1 NC_000962.3 Rv0769, (MTCY369.14), len: 248 aa. Probable dehydrogenase/reductase, similar to others, especially short-chain type dehydrogenases/reductases and 3-oxoacyl-(acyl-carrier protein) reductases e.g. NP_106890.1|NC_002678 probable short-chain type dehydrogenase/reductase from Mesorhizobium loti (374 aa); NP_243357.1|NC_002570 3-oxoacyl-(acyl-carrier protein) reductase from Bacillus halodurans (246 aa); P28643|FABG_CUPLA 3-oxoacyl-[acyl-carrier protein] reductase from Cuphea lanceolata (320 aa); P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 536, E(): 6.5e-27, (37.7% identity in 247 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. MTCY02B10.14, FASTA score: (33.7% identity in 249 aa overlap); etc.; Probable dehydrogenase/reductase 863158 888837 Rv0769 Mycobacterium tuberculosis H37Rv Probable dehydrogenase/reductase NP_215283.1 862412 D 83332 CDS NP_215284.1 15607910 888868 863256..864143 1 NC_000962.3 Rv0770, (MTCY369.15), len: 295 aa. Probable dehydrogenase/reductase, 3-hydroxyisobutyrate dehydrogenase family, possibly 3-hydroxyisobutyrate dehydrogenase or 2-hydroxy-3-oxopropionate reductase, similar to others e.g. P23523|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (tartronate semialdehyde reductase) (TSAR) from Escherichia coli strain K12 (294 aa), FASTA scores: opt: 469, E(): 6.7e-22, (34.4% identity in 282 aa overlap); P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (hibadh) from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 439, E(): 4.3e-20, (34.9% identity in 269 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Seems to belong to the 3-hydroxyisobutyrate dehydrogenase family.; Probable dehydrogenase/reductase 864143 888868 Rv0770 Mycobacterium tuberculosis H37Rv Probable dehydrogenase/reductase NP_215284.1 863256 D 83332 CDS NP_215285.1 15607911 888872 864140..864574 1 NC_000962.3 Rv0771, (MTCY369.16), len: 144 aa. Possible 4-carboxymuconolactone decarboxylase, showing similarity with other carboxymuconolactone decarboxylases e.g. AAD39557.1|AF031417 PcaC-like protein from Pseudomonas putida (130 aa); P20370|DC4C_ACICA 4-carboxymuconolactone decarboxylase (CMD) from Acinetobacter sp. ADP1 (134 aa),FASTA scores: opt: 174, E(): 0.00075, (31.4% identity in 121 aa overlap); C-terminus of NP_421214.1|NC_002696 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Caulobacter crescentus (393 aa); C-terminus of T47115 probable 4-carboxymuconolactone decarboxylase / 3-oxoadipate enol-lactone hydrolase from Streptomyces sp (373 aa); NP_407104.1|NC_003143 putative gamma carboxymuconolactone decarboxylase from Yersinia pestis (131 aa); etc.; Possible 4-carboxymuconolactone decarboxylase (CMD) 864574 888872 Rv0771 Mycobacterium tuberculosis H37Rv Possible 4-carboxymuconolactone decarboxylase (CMD) NP_215285.1 864140 D 83332 CDS NP_215286.1 15607912 888873 864586..865854 1 NC_000962.3 Rv0772, (MTCY369.17), len: 422 aa. Probable purD,phosphoribosylamine--glycine ligase, equivalent to Q50144|PURD|PUR2_MYCLE|ML2235|MLCB5.08 phosphoribosylamine--glycine ligase from Mycobacterium leprae (422 aa), FASTA scores: opt: 2272, E(): 0, (81.8% identity in 422 aa overlap). Also highly similar to others e.g. CAB56348.1|AL118514 phosphoribosylamine-glycine ligase from Streptomyces coelicolor (416 aa); P1564|PUR2_ECOLI phosphoribosylamine--glycine ligase from Escherichia coli (429 aa), FASTA scores: opt: 1039, E(): 0, (42.7% identity in 431 aa overlap); etc. Belongs to the GarS family.; Probable phosphoribosylamine--glycine ligase PurD (GARS) (glycinamide ribonucleotide synthetase) (phosphoribosylglycinamide synthetase) (5'-phosphoribosylglycinamide synthetase) 865854 purD 888873 purD Mycobacterium tuberculosis H37Rv Probable phosphoribosylamine--glycine ligase PurD (GARS) (glycinamide ribonucleotide synthetase) (phosphoribosylglycinamide synthetase) (5'-phosphoribosylglycinamide synthetase) NP_215286.1 864586 D 83332 CDS NP_215287.1 15607913 888893 complement(865851..867389) 1 NC_000962.3 Rv0773c, (MTCY369.18), len: 512 aa. Probable ggtA,bifunctional acylase including cephalosporin acylase, and gamma-glutamyl transpeptidase; highly similar to others e.g. NP_295247.1|NC_001263 cephalosporin acylase from Deinococcus radiodurans (535 aa); NP_248854.1|NC_002516 probable gamma-glutamyltranspeptidase from Pseudomonas aeruginosa (538 aa); P15557|PAC1_PSES3 acylase ACY 1 [includes: cephalosporin acylase (GL-7ACA acylase); gamma-glutamyltranspeptidase (GGT)] from Pseudomonas sp. strain SE83 (558 aa), FASTA scores: opt: 784, E(): 0,(34.2% identity in 526 aa overlap); NP_391491.1|NC_000964|Z93767|BSZ93767_6|O0521 protein similar to gamma-glutamyltransferase from Bacillus subtilis (525 aa), FASTA scores: opt: 1169, E(): 0, (40.1% identity in 516 aa overlap); etc. Also similar to Rv2394|ggtB from Mycobacterium tuberculosis. Member of GL-7ACA acylases and to GGT group.; Probable bifunctional acylase GgtA: cephalosporin acylase (GL-7ACA acylase) + gamma-glutamyltranspeptidase (GGT) 867389 ggtA 888893 ggtA Mycobacterium tuberculosis H37Rv Probable bifunctional acylase GgtA: cephalosporin acylase (GL-7ACA acylase) + gamma-glutamyltranspeptidase (GGT) NP_215287.1 865851 R 83332 CDS NP_215288.2 57116780 888895 complement(867440..868351) 1 NC_000962.3 Rv0774c, (MTCY369.19c), len: 303 aa. Possible conserved exported protein with hydrophobic region near N-terminus, highly similar, except in N-terminus, to Rv0519c|Z97831|MTY20G10.09c|O33364 hypothetical protein from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature, and PS00120 Lipases, serine active site. So could be a lipase. Start changed since first submission (-9 aa). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved exported protein 868351 888895 Rv0774c Mycobacterium tuberculosis H37Rv Probable conserved exported protein NP_215288.2 867440 R 83332 CDS NP_215289.1 15607915 888899 868407..869030 1 NC_000962.3 Rv0775, (MTCY369.20), len: 207 aa. Conserved hypothetical protein, showing some similarity to other proteins e.g. ECAE000186_11|MG1655 hypothetical protein from Escherichia coli strain K-12 (178 aa), FASTA scores: E(): 6.4e-05, (27.2% identity in 147 aa overlap); P41037|BIH_ECOLI hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 138,E(): 0.003, (30.9% identity in 97 aa overlap); etc.; hypothetical protein 869030 888899 Rv0775 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215289.1 868407 D 83332 CDS NP_215290.1 15607916 888918 complement(868984..869763) 1 NC_000962.3 Rv0776c, (MTCY369.21a), len: 259 aa. Conserved hypothetical protein, similar (except first 50 aa) to P72737|D90900_57 hypothetical protein from Synechocystis sp. strain PCC 6803 (261 aa), FASTA scores: opt: 337, E(): 1.7e-15, (30.5% identity in 266 aa overlap).; hypothetical protein 869763 888918 Rv0776c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215290.1 868984 R 83332 CDS NP_215291.1 15607917 888929 870008..871426 1 NC_000962.3 Rv0777, (MTCY369.21b), len: 472 aa. Probable purB,adenylosuccinate lyase, equivalent (but shorter 15 aa) to MLCB5.13|Z95151|g2076607|PURB adenylosuccinate lyase from Mycobacterium leprae (487 aa), FASTA scores: opt: 2640,E(): 0, (86.7% identity in 472 aa overlap). More similar to eukaryotic adenylosuccinate lyases than to prokaryotic adenylosuccinate lyases e.g. P54822|PUR8_MOUSE adenylosuccinate lyase from Mus musculus (484 aa), FASTA scores: opt: 762, E(): 0, (32.4% identity in 445 aa overlap); CAB99134.1|AL390188 putative adenylosuccino lyase (fragment) from Streptomyces coelicolor (362 aa); etc. Contains PS00163 Fumarate lyases signature. Belongs to the lyase 1 family, adenylossucinate lyase subfamily.; Probable adenylosuccinate lyase PurB (adenylosuccinase) (ASL) (ASASE) 871426 purB 888929 purB Mycobacterium tuberculosis H37Rv Probable adenylosuccinate lyase PurB (adenylosuccinase) (ASL) (ASASE) NP_215291.1 870008 D 83332 CDS NP_215292.1 15607918 888913 871431..872675 1 NC_000962.3 Rv0778, (MT0802, MTCY369.22), len: 414 aa. Possible cyp126, cytochrome P-450, similar to other cytochromes and related proteins e.g. AAG29781.1|AF235050_4|AF235050 cytochrome P-450 from Streptomyces rishiriensis (407 aa); Q59723|PSECYTOCHR_1 cytochrome p-450 linalool 8-monooxygenase (lin C) from Pseudomonas incognita (406 aa), FASTA scores: opt: 769, E(): 0, (37.0% identity in 411 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0766c, Rv2266, Rv3545c, etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature.; Possible cytochrome P450 126 Cyp126 872675 cyp126 888913 cyp126 Mycobacterium tuberculosis H37Rv Possible cytochrome P450 126 Cyp126 NP_215292.1 871431 D 83332 CDS NP_215293.1 15607919 888941 complement(872672..873292) 1 NC_000962.3 Rv0779c, (MTCY369.23c), len: 206 aa. Possible conserved transmembrane protein, equivalent to Z95151|MLCB5_14 O05747 conserved hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 902, E(): 0, (67.2% identity in 204 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved transmembrane protein 873292 888941 Rv0779c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_215293.1 872672 R 83332 CDS NP_215294.1 15607920 888928 873343..874236 1 NC_000962.3 Rv0780, (MTCY369.24), len: 297 aa. PurC,phosphoribosylaminoimidazole- succinocarboxamide synthase (see citations below), equivalent to MTU34957_1|PURC phosphoribosylaminoimidazole-succinocarboxamide synthase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1986, E(): 0, (99.3% identity in 297 aa overlap). Also similar to others e.g. CAB56351.1|AL118514 phosphoribosylaminoimidazole-succinocarboxamide synthase from Streptomyces coelicolor (299 aa); etc. Contains PS01058 SAICAR synthetase signature 2. Belongs to the SAICAR synthetase family.; Phosphoribosylaminoimidazole-succinocarboxamide synthase PurC (SAICAR synthetase) 874236 purC 888928 purC Mycobacterium tuberculosis H37Rv Phosphoribosylaminoimidazole-succinocarboxamide synthase PurC (SAICAR synthetase) NP_215294.1 873343 D 83332 CDS NP_215296.2 57116781 885840 874233..874943 1 NC_000962.3 Rv0781, (MTCY369.25), len: 236 aa. Probable ptrBa,first part of protease II, equivalent to N-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 204, E(): 7.4e-07, (29.6% identity in 230 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBb. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable protease II PtrBa [first part] (oligopeptidase B) 874943 ptrBa 885840 ptrBa Mycobacterium tuberculosis H37Rv Probable protease II PtrBa [first part] (oligopeptidase B) NP_215296.2 874233 D 83332 CDS NP_215295.2 57116782 885862 874732..876390 1 NC_000962.3 Rv0782, (MTCY369.26), len: 552 aa. Probable ptrBb,second part of protease II, equivalent to C-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 1251, E(): 0, (42.7% identity in 489 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBa. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable protease II PtrBb [second part] (oligopeptidase B) 876390 ptrBb 885862 ptrBb Mycobacterium tuberculosis H37Rv Probable protease II PtrBb [second part] (oligopeptidase B) NP_215295.2 874732 D 83332 CDS NP_215297.1 15607923 885836 complement(876818..878440) 1 NC_000962.3 Rv0783c, (MTCY369.27c), len: 540 aa. Possible emrB,integral membrane drug efflux protein, member of major facilitator superfamily (MFS), equivalent to AAL16083.1|AF421382_1|AF421382 EmrB efflux protein from Mycobacterium avium (538 aa). Also similar to other membrane proteins e.g. CAB61606.1|AL133210 putative export protein from Streptomyces coelicolor (496 aa); NP_108371.1|NC_002678 efflux pump protein FarB from Mesorhizobium loti (511 aa); P44927|EMRB_HAEINHI0897| multidrug resistance protein b homologue from Haemophilus influenzae (510 aa), FASTA scores: opt: 706, E(): 1.3e-36,(30.4% identity in 408 aa overlap); etc. Also similar to Rv2333c|MTCY3G12.01 from Mycobacterium tuberculosis (537 aa), FASTA score: (28.2% identity in 408 aa overlap); and Rv1410c|MTCY21B4.27c from Mycobacterium tuberculosis (518 aa), FASTA score: (26.8% identity in 496 aa overlap). Belongs to the major facilitator family; also known as the drug resistance translocase family.; Possible multidrug resistance integral membrane efflux protein EmrB 878440 emrB 885836 emrB Mycobacterium tuberculosis H37Rv Possible multidrug resistance integral membrane efflux protein EmrB NP_215297.1 876818 R 83332 CDS NP_215298.1 15607924 885863 878638..879324 1 NC_000962.3 Rv0784, (MTC369.28), len: 228 aa. Conserved hypothetical protein, with some similarity to MLCB5_20|O05752 hypothetical protein from Mycobacterium leprae (193 aa), FASTA scores: opt: 141, E(): 0.0022,(36.0% identity in 114 aa overlap). Also similar to N-terminus of NP_253002.1|NC_002516 conserved hypothetical protein from Pseudomonas aeruginosa (253 aa).; hypothetical protein 879324 885863 Rv0784 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215298.1 878638 D 83332 CDS NP_215299.1 15607925 885864 879340..881040 1 NC_000962.3 Rv0785, (MTCY369.29), len: 566 aa. Conserved protein, highly similar to other conserved hypothetical proteins e.g. NP_105777.1| NC_002678 hypothetical protein from Mesorhizobium loti (552 aa); SC5F8.14|CAB93742.1|AL357613 conserved hypothetical protein from Streptomyces coelicolor (557 aa); AE001863|AE001863_31 from Deinococcus radiodurans (554 aa), FASTA scores: opt: 2243, E(): 0, (61.1% identity in 550 aa overlap); YEF7_YEAST|P32614 hypothetical 50.8 kd protein (470 aa),FASTA scores: opt: 169, E(): 0.0014, (23.8% identity in 542 aa overlap); etc. Also similar to Rv1817|MTCY1A11.26c from Mycobacterium tuberculosis (487 aa), FASTA score: (26.7% identity in 587 aa overlap). And shows similarity with other dehydrogenases.; hypothetical protein 881040 885864 Rv0785 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215299.1 879340 D 83332 CDS NP_215300.1 15607926 885615 complement(881075..881464) 1 NC_000962.3 Rv0786c, (MTCY369.30c), len: 129 aa. Conserved protein, similar to three other hypothetical proteins from Streptomyces coelicolor e.g. SC7H1.08c|T35703 hypothetical protein (202 aa), FASTA scores: opt: 241, E(): 5.1e-10,(41.0% identity in 105 aa overlap); SC3A7.08|T29426 (211 aa).; hypothetical protein 881464 885615 Rv0786c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215300.1 881075 R 83332 CDS NP_215301.1 15607927 885468 881459..882418 1 NC_000962.3 Rv0787, (MTCY369.31), len: 319 aa. Unknown protein,equivalent to AAK45053.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 77 aa.; hypothetical protein 882418 885468 Rv0787 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215301.1 881459 D 83332 CDS YP_177755.1 57116783 886264 882524..882763 1 NC_000962.3 Rv0787A, len: 79 aa. Conserved protein, equivalent to MLCB5.24 hypothetical protein from Mycobacterium leprae (79 aa), FASTA scores: opt: 434, (84.8% identity in 79 aa overlap). Also similar to P12049|YEXA_BACSU hypothetical 9.7 kDa protein from Bacillus subtilis (84 aa), FASTA scores: opt: 172, E(): 4e-06, (44.4% identity in 72 aa overlap). Belongs to the UPF0062 family.; hypothetical protein 882763 886264 Rv0787A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177755.1 882524 D 83332 CDS NP_215303.1 15607928 885181 882760..883434 1 NC_000962.3 Rv0788, (MTCY369.32), len: 224 aa. Probable purQ,phosphoribosylformylglycinamidine synthase I, equivalent to MLCB5_24|Z95151|O05756|PURQ phosphoribosylformylglycinamidine synthase I from Mycobacterium leprae (224 aa), FASTA scores: opt: 1341,E(): 0, (88.7% identity in 222 aa overlap). Also highly similar to others e.g. P12041|PURQ_BACSU phosphoribosylformylglycinamidine synthase I from Bacillus subtilis (227 aa), FASTA scores: opt: 691, E(): 8.6e-39,(47.7% identity in 214 aa overlap); etc. Contains PS00442 Glutamine amidotransferases class-I active site. Belongs to type-1 glutamine amidotransferases.; Probable phosphoribosylformylglycinamidine synthase I PURG (FGAM synthase I) 883434 purQ 885181 purQ Mycobacterium tuberculosis H37Rv Probable phosphoribosylformylglycinamidine synthase I PURG (FGAM synthase I) NP_215303.1 882760 D 83332 CDS NP_215304.1 15607929 885153 complement(883451..884050) 1 NC_000962.3 Rv0789c, (MTCY369.33c), len: 199 aa. Hypothetical unknown protein.; Hypothetical protein 884050 885153 Rv0789c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215304.1 883451 R 83332 CDS NP_215305.1 15607930 885253 complement(884072..884800) 1 NC_000962.3 Rv0790c, (MTCY369.34c), len: 242 aa. Hypothetical unknown protein.; Hypothetical protein 884800 885253 Rv0790c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215305.1 884072 R 83332 CDS NP_215306.1 15607931 885273 complement(884797..885840) 1 NC_000962.3 Rv0791c, (MTV042.01c, MTCY369.35c), len: 347 aa. Conserved protein, similar (except in N-terminus) to others e.g. CAC44585.1|AL596162 conserved hypothetical protein from Streptomyces coelicolor (307 aa); NP_252643.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (364 aa); etc. Also some similarity with oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); etc. And also similar in part to other proteins from Mycobacterium tuberculosis e.g. Rv1855c|MTCY359.18|Z83859 (307 aa), FASTA scores: opt: 366,E(): 4e-16, (35.0% identity in 226 aa overlap); Rv3079c|MTCY22D7.02|Z83866 conserved hypothetical protein (275 aa), FASTA scores: opt: 342, E(): 1.2e-14, (31.6% identity in 234 aa overlap); Rv0044c possible oxidoreductase (264 aa).; hypothetical protein 885840 885273 Rv0791c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215306.1 884797 R 83332 CDS NP_215307.1 15607932 885142 complement(885837..886646) 1 NC_000962.3 Rv0792c, (MTV042.02c), len: 269 aa. Probable transcriptional regulator, GntR-family, similar to many others of GntR family e.g. BSUB0018_189|Z99121 from Bacillus subtilis (243 aa), FASTA scores: opt: 367, E(): 1.5e-17, (32.1% identity in 246 aa overlap); P31453|YIDP_ECOLI from Escherichia coli (238 aa), FASTA scores: opt: 236, E(): 8.8e-09, (26.4% identity in 235 aa overlap); etc.; Probable transcriptional regulatory protein (probably GntR-family) 886646 885142 Rv0792c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably GntR-family) NP_215307.1 885837 R 83332 CDS NP_215308.1 15607933 885497 886719..887024 1 NC_000962.3 Rv0793, (MTV042.03), len: 101 aa. Possible monooxygenase (See Lemieux et al., 2005). Similar to e.g. NP_250888.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (114 aa); AE 001908|AE001908_7 hypothetical protein from Deinococcus radiodurans (101 aa), FASTA scores: opt: 215, E(): 3.1e-09, (40.4% identity in 99 aa overlap); NP_440966.1|NC_000911|D90908|PCC6803|D90908_2 unknown protein from Synechocystis sp. strain PCC 6803 (147 aa), FASTA scores: opt: 194, E(): 4.5e-08, (31.1% identity in 90 aa overlap); etc. Also similar to Rv2749|MTV002.14|AL0089|MTV002_15 conserved hypothetical protein from Mycobacterium tuberculosis (104 aa), FASTA scores: opt: 143, E(): 0.00026, (26.9% identity in 93 aa overlap).; Possible monooxygenase 887024 885497 Rv0793 Mycobacterium tuberculosis H37Rv Possible monooxygenase NP_215308.1 886719 D 83332 CDS YP_177756.1 57116784 885076 complement(887137..888636) 1 NC_000962.3 Rv0794c, (MTV042.04c), len: 499 aa. Probable oxidoreductase, possibly dihydrolipoamide dehydrogenase or mercuric reductase. Highly similar to CAB62675.1|AL133422 probable oxidoreductase from Streptomyces coelicolor (477 aa); and similar to various oxidoreductases e.g. P08663|MERA_STAAU mercuric reductase (HG(II) reductase) from Staphylococcus aureus (547 aa); AAK70920.1|AC087551_19|AC087551 putative lipoamide dehydrogenase from Oryza sativa (563 aa); NP_437349.1|NC_003078 putative FAD-dependent pyridine nucleotide-disulphide oxidoreductase, similar to mercuric reductases protein from Sinorhizobium meliloti (473 aa); Q04829|DLDH_HALVO dihydrolipoamide dehydrogenase from Haloferax volcanii (475 aa); P08332|MERA_SHIFL mercuric reductase (564 aa), FASTA scores: opt: 522, E(): 3.7e-26,(31.7% identity in 467 aa overlap); P72740|DLDH_SYNY3|Q53395|LPDA|PDHD|SLR1096 dihydrolipoamide dehydrogenase from Synechocystis sp. strain PCC 6803 (474 aa), FASTA scores: opt: 602, E(): 2.3e-31, (31.0% identity in 493 aa overlap); etc. Note that previously known as lpdB.; Probable oxidoreductase 888636 885076 Rv0794c Mycobacterium tuberculosis H37Rv Probable oxidoreductase YP_177756.1 887137 R 83332 CDS NP_215310.1 15607935 885454 889072..889398 1 NC_000962.3 Rv0795, (MTV042.05), len: 108 aa. Putative transposase for IS6110 (fragment), identical to Q50686 insertion element IS6110 (108 aa), FASTA score: (100.0 % identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Putative transposase for insertion sequence element IS6110 (fragment) 889398 885454 Rv0795 Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 (fragment) NP_215310.1 889072 D 83332 CDS NP_215311.2 448824749 885099 <889347..890333 1 NC_000962.3 Rv0796, (MTV042.06), len: 328 aa. Putative transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 50 aa).; Putative transposase for insertion sequence element IS6110 890333 885099 Rv0796 Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 NP_215311.2 <889347 D 83332 CDS NP_215312.1 15607937 885476 890388..891482 1 NC_000962.3 Rv0797, (MTCI249B.03c, MTV042.07), len: 364 aa. Putative transposase for IS1547; almost identical to (but 20 aa shorter than) Y13470|MTY13470_2 from Mycobacterium tuberculosis (383 aa). Also similar to other transposases e.g. MAIS1110A _1|Q48909 transposase from Mycobacterium avium (464 aa), FASTA scores: opt: 226, E(): 2.4e-08,(30.7% identity in 199 aa overlap). Also slight similarity to Rv2014|MTCY39.03c from Mycobacterium tuberculosis (222 aa), FASTA score: (24.8% identity in 141 aa overlap).; Putative transposase for insertion sequence element IS1547 891482 885476 Rv0797 Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS1547 NP_215312.1 890388 D 83332 CDS NP_215313.1 15607938 885460 complement(891472..892269) 1 NC_000962.3 Rv0798c, (MTCI429B.02), len: 265 aa. Cfp29, 29 kDa antigen (see citations below). Highly similar to Q45296|BLLINM18P_1|CAA63787.1|X93588 linocin M18 from Brevibacterium linens (266 aa), FASTA scores: (58.5% identity in 265 aa overlap). Also shows similarity with NP_228594.1|NC_000853 bacteriocin from Thermotoga maritima (262 aa).; 29 KDa antigen CFP29 892269 cfp29 885460 cfp29 Mycobacterium tuberculosis H37Rv 29 KDa antigen CFP29 NP_215313.1 891472 R 83332 CDS NP_215314.1 15607939 885388 complement(892266..893273) 1 NC_000962.3 Rv0799c, (MTCY07H7A.10, MTCI429B.01), len: 335 aa. Conserved protein, similar to Q50021|U2266C from Mycobacterium leprae (146 aa), FASTA scores: opt: 147, E(): 0.0016, (33.3% identity in 117 aa overlap); Q50020|U2266B from Mycobacterium leprae (27 aa), FASTA scores: opt: 94,E(): 1.3, (56.5% identity in 23 aa overlap). Also highly similar to others e.g. CAC01593.1|AL391041 conserved hypothetical protein from Streptomyces coelicolor (316 aa); AF088897|AF088897_9 hypothetical protein from Zymomonas mobilis (322 aa), FASTA scores: opt: 1132, E(): 0, (56.1% identity in 303 aa overlap); P76536|ECAE000330_8 hypothetical protein from Escherichia coli strain K-12 (308 aa), FASTA scores: E(): 2.2e-30, (37.4% identity in 297 aa overlap); etc. Also similar to some tyrA proteins. Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 893273 885388 Rv0799c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215314.1 892266 R 83332 CDS NP_215315.1 15607940 885461 893318..894619 1 NC_000962.3 Rv0800, (MTCY07H7A.09c), len: 433 aa. Probable pepC,aminopeptidase I, highly similar (but shorter 17 aa) to Q50022|PEPX aminopeptidase from Mycobacterium leprae (443 aa), FASTA scores: opt: 2237, E(): 0, (78.3% identity in 433 aa overlap). Also highly similar to others from Eukaryotes and bacteria, e.g. T36482 probable aminopeptidase from Streptomyces coelicolor (432 aa),P14904|AMPL_YEAST vacuolar aminopeptidase I precursor from Saccharomyces cerevisiae (514 aa), FASTA scores: opt: 425,E(): 4.8e-21, (31.0% identity in 445 aa overlap); etc. Also similar to hypothetical proteins e.g. P38821|YHR3_YEAST hypothetical 54.2 kDa protein from Saccharomyces cerevisiae (490 aa), FASTA scores: opt: 429, E(): 2.5e-21, (34.8% identity in 443 aa overlap); etc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable aminopeptidase PepC 894619 pepC 885461 pepC Mycobacterium tuberculosis H37Rv Probable aminopeptidase PepC NP_215315.1 893318 D 83332 CDS NP_215316.1 15607941 885376 894631..894978 1 NC_000962.3 Rv0801, (MTCY07H7A.08c), len: 115 aa. Conserved protein, similar to many hypothetical proteins from Streptomyces sp. e.g. SCD840A.20|AB81865.1|AL161691 hypothetical protein from Streptomyces coelicolor (145 aa); AF072709|AF072709_8 from Streptomyces lividans (131 aa),FASTA scores: opt: 120, E(): 0.2, (26.3% identity in 118 aa overlap); etc.; hypothetical protein 894978 885376 Rv0801 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215316.1 894631 D 83332 CDS NP_215317.1 15607942 885332 complement(894972..895628) 1 NC_000962.3 Rv0802c, (MTCY07H7A.07c), len: 218 aa. Possible succinyltransferase in the GNAT (Gcn5-related N-acetyltransferase) family (See Vetting et al., 2008). Shows partial similarity with many acetyltransferases and hypothetical proteins e.g. P96579|BSUB0003_68 probable acetyltransferase from Bacillus subtilis (183 aa), FASTA scores: E(): 0.0044, (26.4% identity in 110 aa overlap).; Possible succinyltransferase in the GCN5-related N-acetyltransferase family 895628 885332 Rv0802c Mycobacterium tuberculosis H37Rv Possible succinyltransferase in the GCN5-related N-acetyltransferase family NP_215317.1 894972 R 83332 CDS NP_215318.1 15607943 885358 895820..898084 1 NC_000962.3 Rv0803, (MTCY07H7A.06c), len: 754 aa. PurL,phosphoribosylformylglycinamidine synthase II (see citations below), equivalent to NP_302451.1|NC_002677 phosphoribosylformylglycinamidine synthase II from Mycobacterium leprae (754 aa). Also highly similar to others e.g. Q9RKK5|PURL_STRCO from Streptomyces coelicolor (752 aa); P12042|PURL_BACSU from Bacillus subtilis (742 aa), FASTA score: (44.7% identity in 716 aa); etc. Start was chosen by similarity. Belongs to the FGAMS family.; Phosphoribosylformylglycinamidine synthase II PurL (FGAM synthase II) 898084 purL 885358 purL Mycobacterium tuberculosis H37Rv Phosphoribosylformylglycinamidine synthase II PurL (FGAM synthase II) NP_215318.1 895820 D 83332 CDS NP_215319.1 15607944 885330 898081..898710 1 NC_000962.3 Rv0804, (MTCY07H7A.05c), len: 209 aa. Conserved hypothetical protein, showing similarity with C-terminus of Rv1863c|MTCY359.10 conserved hypothetical protein from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 199, E(): 1.2e-05, (33.2% identity in 220 aa overlap); and Rv0658c. Contains PS01151 Fimbrial biogenesis outer membrane usher protein signature.; hypothetical protein 898710 885330 Rv0804 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215319.1 898081 D 83332 CDS NP_215320.1 15607945 885326 898831..899787 1 NC_000962.3 Rv0805, (MTCY07H7A.04c), len: 318 aa. Cyclic nucleotide phosphodiesterase (cNMP PDE) (See Shenoy et al.,2005), member of binuclear metallophosphoesterase superfamily, equivalent to Q50024 from Mycobacterium leprae (317 aa), FASTA scores: opt: 1713, E(): 0, (82.5% identity in 315 aa overlap). Also shows similarity with hypothetical proteins and icc proteins e.g. SC9B1.22c|T35867 hypothetical protein from Streptomyces coelicolor (305 aa); P36650|ICC_ECOLI icc protein from Escherichia coli (275 aa), FASTA scores: opt: 310, E(): 8.9e-14, (31.3% identity in 214 aa overlap); etc.; Class III cyclic nucleotide phosphodiesterase (cNMP PDE) 899787 885326 Rv0805 Mycobacterium tuberculosis H37Rv Class III cyclic nucleotide phosphodiesterase (cNMP PDE) NP_215320.1 898831 D 83332 CDS NP_215321.1 15607946 885243 complement(899732..901330) 1 NC_000962.3 Rv0806c, (MTCY07H7A.03), len: 532 aa. Possible cpsY,UDP-glucose-4-epimerase, equivalent to Q50025|CPSY probable UDP-glucose-4-epimerase from Mycobacterium leprae (542 aa),FASTA scores: opt: 2964, E(): 0, (82.3% identity in 530 aa overlap). Also similar to AAC38286.1|AF019760|SACB CpsY homolog (involved in meningococcal capsule biosynthesis) from Neisseria meningitidis serogroup a (545 aa); Q51151 capsule gene complex UPD-glucose-4-epimerase (gale) from Neisseria meningitidis (373 aa), FASTA scores: opt: 496,E(): 9.5e-27, (29.3% identity in 358 aa overlap); C-terminus of CAB75373.1|AL139298 putative transferase from Streptomyces coelicolor (942 aa); and many hypothetical proteins from Streptomyces coelicolor. Seems to belong to the sugar epimerase family.; Possible UDP-glucose-4-epimerase CpsY (galactowaldenase) (UDP-galactose-4-epimerase) (uridine diphosphate galactose-4-epimerase) (uridine diphospho-galactose-4-epimerase) 901330 cpsY 885243 cpsY Mycobacterium tuberculosis H37Rv Possible UDP-glucose-4-epimerase CpsY (galactowaldenase) (UDP-galactose-4-epimerase) (uridine diphosphate galactose-4-epimerase) (uridine diphospho-galactose-4-epimerase) NP_215321.1 899732 R 83332 CDS NP_215322.1 15607947 885272 901635..902024 1 NC_000962.3 Rv0807, (MTCY07H7A.02c), len: 129 aa. Conserved hypothetical protein, equivalent to O05761|MLCB5_31 hypothetical 14.0 kDa protein from Mycobacterium leprae (131 aa), FASTA scores: E(): 0, (73.4% identity in 128 aa overlap). Also highly similar to BAA89438.1|AB003158|ORF3 hypothetical protein from Corynebacterium ammoniagenes (132 aa); and C-terminus of SCD25.20|CAB56364.1|AL118514 hypothetical protein from Streptomyces coelicolor (202 aa).; hypothetical protein 902024 885272 Rv0807 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215322.1 901635 D 83332 CDS NP_215323.1 15607948 885085 902111..903694 1 NC_000962.3 Rv0808, (MTCY07H7A.01c), len: 527 aa. PurF,amidophosphoribosyltransferase, equivalent to MLCB5_32|Q50028|PURF from Mycobacterium leprae (556 aa),FASTA scores: (91.3% identity in 518 aa overlap); and CAB96578.1|AJ278609 phosphoribosyl pyrophosphate amidotransferase from Mycobacterium smegmatis (511 aa)(see citation below). Also highly similar to others e.g. BAA89439.1|AB003158 amidophosphoribosyl transferase from Corynebacterium ammoniagenes (490 aa); P00497|PUR1_BACSU amidophosphoribosyltransferase precursor from Bacillus subtilis (476 aa), FASTA scores: opt: 1412, E(): 0, (46.2% identity in 470 aa overlap); etc. Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family.; Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase) 903694 purF 885085 purF Mycobacterium tuberculosis H37Rv Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase) NP_215323.1 902111 D 83332 CDS NP_215324.1 15607949 885134 903725..904819 1 NC_000962.3 Rv0809, (MTV043.01), len: 364 aa. Probable purM,5'-phosphoribosyl-5-aminoimidazole synthetase, equivalent to NP_302446.1|NC_002677 5'-phosphoribosyl-5-aminoimidazole synthase from Mycobacterium leprae (364 aa). Also highly similar to many e.g. P12043|PUR5_BACSU phosphoribosylformylglycinamidine CYCLO-ligase from Bacillus subtilis (346 aa), FASTA scores: opt: 1023, E(): 0, (46.5% identity in 331 aa overlap); U68765|STU68765_2 from Salmonella typhimurium (345 aa), FASTA scores: opt: 1014, E():0, (47.6% identity in 330 aa overlap); etc.; Probable phosphoribosylformylglycinamidine CYCLO-ligase PurM (AIRS) (phosphoribosyl-aminoimidazole synthetase) (air synthase) 904819 purM 885134 purM Mycobacterium tuberculosis H37Rv Probable phosphoribosylformylglycinamidine CYCLO-ligase PurM (AIRS) (phosphoribosyl-aminoimidazole synthetase) (air synthase) NP_215324.1 903725 D 83332 CDS NP_215325.1 15607950 885410 complement(904905..905087) 1 NC_000962.3 Rv0810c, (MTV043.02c), len: 60 aa. Conserved hypothetical protein, with its N-terminus highly similar to NP_302445.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (62 aa); and AL118514|SCD25_24 hypothetical protein from Streptomyces coelicolor (84 aa),FASTA scores: opt: 180, E(): 5.7e-07, (51.8% identity in 56 aa overlap).; hypothetical protein 905087 885410 Rv0810c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215325.1 904905 R 83332 CDS NP_215326.1 15607951 885401 complement(905234..906340) 1 NC_000962.3 Rv0811c, (MTV043.03c), len: 368 aa. Conserved protein, equivalent to U2266F|U15182|MLU15182_13 hypothetical protein from Mycobacterium leprae (366 aa),FASTA scores: opt: 1870, E(): 0, (77.4% identity in 367 aa overlap). Also highly similar to BAA89441.1|AB003158|ORF4 hypothetical protein from Corynebacterium ammoniagenes (359 aa); and CAB94085.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (321 aa).; hypothetical protein 906340 885401 Rv0811c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215326.1 905234 R 83332 CDS YP_177757.1 57116785 885397 906423..907292 1 NC_000962.3 Rv0812, (MTV043.04), len: 289 aa. Probable amino acid aminotransferase, similar to other amino acid aminotransferases, generelly class-IV of pyridoxal-phosphate-dependent aminotransferases, and especially ILVE proteins and PABC proteins e.g. B76065.1|AL157953 putative aminotransferase from Streptomyces coelicolor (273 aa); NP_069766.1|NC_000917 branched-chain amino acid aminotransferase (ilvE) from Archaeoglobus fulgidus (290 aa); P54692|DAAA_BACLI D-alanine aminotransferase from Bacillus licheniformis (283 aa); P28305|PABC_ECOLI|B1096 4-amino-4-deoxychorismate lyase (ADC lyase) From Escherichia coli strain K12 (269 aa), FASTA scores: opt: 165, E(): 0.00064, (26.8% identity in 198 aa overlap); etc. Note that previously known as pabC.; Probable amino acid aminotransferase 907292 885397 Rv0812 Mycobacterium tuberculosis H37Rv Probable amino acid aminotransferase YP_177757.1 906423 D 83332 CDS NP_215328.1 15607953 885395 complement(907338..908018) 1 NC_000962.3 Rv0813c, (MTV043.05c), len: 226 aa. Conserved protein, highly similar to U15182|MLU15182_16 hypothetical protein from Mycobacterium leprae (242 aa), FASTA scores: opt: 1191, E(): 0, (78.3% identity in 226 aa overlap); and NP_302442.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (228 aa). Also similar to AB94083.1|AL358692|SCD66.16 hypothetical protein from Streptomyces coelicolor (191 aa); and Rv2717c|MTCY05A6_37 hypothetical protein from Mycobacterium tuberculosis (164 aa), FASTA score: (30.4% identity in 171 aa overlap). Possibly a new bacterial family of fatty acid-binding protein-like proteins (See Shepard et al., 2007).; hypothetical protein 908018 885395 Rv0813c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215328.1 907338 R 83332 CDS NP_215329.1 15607954 885435 complement(908181..908483) 1 NC_000962.3 Rv0814c, (MTV043.06c, O05794), len: 100 aa. SseC2,conserved protein, highly similar to AAA62972.1|U15182|MLU15182_17 hypothetical protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 545, E(): 0, (84.0% identity in 100 aa overlap); and NP_302441.1|NC_002677|Z95150|MTCY164_29 conserved hypothetical protein from Mycobacterium leprae (100 aa),FASTA scores: opt: 647, E(): 0, (100.0% identity in 100 aa overlap). Also highly similar to M29612|SERCYSA_5 rhodanese-like protein from Saccharopolyspora erythraea (101 aa), FASTA scores: opt: 345, E(): 1.2e-18, (57.1% identity in 98 aa overlap); and similar at the C-terminus to the C-terminus of CAB94069.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (95 aa). Identical second copy present as Rv3118|MTCY164.28|SSEC1 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap).; Conserved protein SseC2 908483 sseC2 885435 sseC2 Mycobacterium tuberculosis H37Rv Conserved protein SseC2 NP_215329.1 908181 R 83332 CDS NP_215330.1 15607955 885449 complement(908485..909318) 1 NC_000962.3 Rv0815c, (MTV043.07c, MT0837, O05793), len: 277 aa. Probable cysA2 (alternate gene name: sseC4), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER putative thiosulfate sulfurtransferase from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa); NP_293941.1|NC_001263 thiosulfate sulfurtransferase from Deinococcus radiodurans (286 aa); etc. Identical second copy present as Rv3117|MTCY164.27|MT3199|O05793|cysA3 (277 aa) (100.0% identity in 277 aa overlap). Contains PS00683 Rhodanese C-terminal signature at C-terminus. Belongs to the rhodanese family.; Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) 909318 cysA2 885449 cysA2 Mycobacterium tuberculosis H37Rv Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) NP_215330.1 908485 R 83332 CDS NP_215331.1 15607956 885087 complement(909611..910033) 1 NC_000962.3 Rv0816c, (MTV043.08c), len: 140 aa. Probable thiX,thioredoxin, equivalent to ThiX|U15182|MLU15182_21 thioredoxin from Mycobacterium leprae (172 aa), FASTA scores: opt: 556, E(): 8.8e-31, (63.8% identity in 141 aa overlap); and similar to AAL08576.1|AF418548_2|AF418548 thioredoxin from Mycobacterium avium subsp. paratuberculosis (117 aa). Also similar to other bacterial thioredoxins e.g. CAB95303.1|AL359779 putative thioredoxin from Streptomyces coelicolor (126 aa); P33791|THIO_STRAU|TRX|TRXA thioredoxin from Streptomyces aureofaciens (106 aa); etc. And similar to Rv3914|MT4033|MTV028.05|NP_218431.1|NC_000962|trxC thioredoxin (TRX) (MPT46) from Mycobacterium tuberculosis (116 aa). Has hydrophobic stretch at N-terminus. Seems to belong to the thioredoxin family.; Probable thioredoxin ThiX 910033 thiX 885087 thiX Mycobacterium tuberculosis H37Rv Probable thioredoxin ThiX NP_215331.1 909611 R 83332 CDS NP_215332.1 15607957 885440 complement(910030..910842) 1 NC_000962.3 Rv0817c, (MTV043.09c), len: 270 aa. Probable conserved exported protein, with N-terminal signal sequence, equivalent (but shorter 13 aa) to U15182|MLU15182_22|U2266M probable exported protein from Mycobacterium leprae (283 aa), FASTA scores: opt: 1287,E(): 0, (73.0% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved exported protein 910842 885440 Rv0817c Mycobacterium tuberculosis H37Rv Probable conserved exported protein NP_215332.1 910030 R 83332 CDS NP_215333.1 15607958 885144 910972..911739 1 NC_000962.3 Rv0818, (MTV043.10), len: 255 aa. Probable transcriptional regulatory protein, highly similar to Q05943|GLNR_STRCO|L03213|STMGLNR_1|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 945, E(): 0, (61.5 identity in 239 aa overlap); and similar to others from other organisms. Also similar to Rv2884|MTCY274.15|Z74024 from Mycobacterium tuberculosis (252 aa), FASTA scores: opt: 662, E(): 0, (47.8% identity in 226 aa overlap).; Transcriptional regulatory protein 911739 885144 Rv0818 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein NP_215333.1 910972 D 83332 CDS NP_215334.1 15607959 885251 911736..912683 1 NC_000962.3 Rv0819, (MTV043.11), len: 315 aa. MshD,acetyltransferase involved in mycothiol synthesis (see Koledin et al., 2002). Contains two GNAT (Gcn5-related N-acetyltransferase) domains. See Vetting et al. 2003,2005, 2006. Equivalent to U2266N|U15182|MLU15182_24 hypothetical protein from Mycobacterium leprae (312 aa),FASTA scores: opt: 1540, E(): 0, (75.2% identity in 314 aa overlap). Also highly similar to CAB88484.1|AL353816 putative acetyltransferase from Streptomyces coelicolor (309 aa).; GCN5-related N-acetyltransferase, MshD 912683 mshD 885251 mshD Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase, MshD NP_215334.1 911736 D 83332 CDS NP_215335.1 15607960 885136 912726..913502 1 NC_000962.3 Rv0820, (MTV043.12), len: 258 aa. Probable phoT,phosphate-transport ATP-binding protein ABC transporter (see citation below), equivalent to PhoT|MLU15182_28|U15182 phosphate transport system ABC transporter from Mycobacterium leprae (258 aa), FASTA scores: opt: 1556,E(): 0, (91.5% identity in 258 aa overlap). Also highly similar to others e.g. CAB88472.1|AL353816 phosphate ABC transport system ATP-binding protein from Streptomyces coelicolor (258 aa); etc. Note that also highly similar to many PstB proteins e.g. AAC15686.1|AF045938|PstB putative ABC transporter nucleotide binding subunit from Mycobacterium smegmatis (258 aa). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable phosphate-transport ATP-binding protein ABC transporter PhoT 913502 phoT 885136 phoT Mycobacterium tuberculosis H37Rv Probable phosphate-transport ATP-binding protein ABC transporter PhoT NP_215335.1 912726 D 83332 CDS NP_215336.1 15607961 885270 complement(913558..914199) 1 NC_000962.3 Rv0821c, (MTV043.13c), len: 213 aa. Probable phoY2,phosphate-transport system regulatory protein, highly similar to PhoY|MLU15182_29|U15182 phosphate transport system regulator from Mycobacterium leprae (222 aa), FASTA scores: opt: 1268, E(): 0, (93.0% identity in 213 aa overlap). Also similar to others e.g. NP_384620.1|NC_003047 probable phosphate transport system transcriptional regulator protein from Sinorhizobium meliloti (237 aa); etc. Also highly similar to MTCI418A.03c|Z96070|PhoY1 probable phosphate transport system transcriptional regulator protein from Mycobacterium tuberculosis (221 aa),FASTA scores: opt: 937, E(): 0, (63.4% identity in 213 aa overlap). Belongs to the PhoU family.; Probable phosphate-transport system transcriptional regulatory protein PhoY2 914199 phoY2 885270 phoY2 Mycobacterium tuberculosis H37Rv Probable phosphate-transport system transcriptional regulatory protein PhoY2 NP_215336.1 913558 R 83332 CDS NP_215337.1 15607962 885374 complement(914257..916311) 1 NC_000962.3 Rv0822c, (MTV043.14c), len: 684 aa. Conserved protein, highly similar in the region between aa 370 - 580 to U2266O|U15182|MLU15182_30 hypothetical protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 819, E(): 0, (60.6% identity in 221 aa overlap). More extended similarity to Rv3267|Z92771|MTCY71_7 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 434, E(): 2.2e-17, (26.6% identity in 541 aa overlap), and Rv3484. Also similar to various proteins, preferiously putative membrane proteins and membrane-bound regulatory proteins e.g. CAC44512.1|AL596138 putative membrane protein from Streptomyces coelicolor (524 aa); U56901|BSU56901_1 regulatory protein from Bacillus subtilis (391 aa), FASTA scores: opt: 225, E(): 1.3e-05, (24.7% identity in 340 aa overlap). Contains hydrophobic stretch (aa ~ 160-195) and PS00041 Bacterial regulatory proteins, araC family signature.; hypothetical protein 916311 885374 Rv0822c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215337.1 914257 R 83332 CDS NP_215338.1 15607963 885380 complement(916477..917646) 1 NC_000962.3 Rv0823c, (MTV043.15c), len: 389 aa. Possible transcriptional regulator (resembles nitrogen regulation protein), equivalent (but longer 24 aa in N-terminus) to MLU15182_31|U15182|NtrB NtrB protein from Mycobacterium leprae (384 aa), FASTA scores: opt: 2070, E(): 0, (82.3% identity in 384 aa overlap) (see citation below). Also highly similar to CAB63312.1|AL133471|SCC82.03c hypothetical protein from Streptomyces coelicolor (406 aa); and to many transcriptional regulators members of UPF0034 family (NIFR3/SMM1) e.g. D26185|BAC180K_143 protein similar to transcriptional regulator (nitrogen regulation protein) from Bacillus subtilis (333 aa), FASTA scores: opt: 609,E(): 1.4e-32, (38.3% identity in 326 aa overlap); NP_349795.1|NC_003030 NifR3 family enzyme from Clostridium acetobutylicum (321 aa); etc. Contains PS01136 Uncharacterized protein family UPF0034 signature.; Possible transcriptional regulatory protein 917646 885380 Rv0823c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_215338.1 916477 R 83332 CDS YP_177758.1 57116786 885444 complement(917734..918750) 1 NC_000962.3 Rv0824c, (MTV043.16c), len: 338 aa. Probable desA1 (alternate gene name: des), acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase) (see Jackson et al.,1997), equivalent to U15182|MLU15182_32 acyl-[ACP] desaturase from Mycobacterium leprae (338 aa), FASTA scores: opt: 1880, E(): 0, (79.9% identity in 338 aa overlap); and highly similar in part to fragment CAB96061.1|AJ250019 Steroyl-ACP-desaturase from Mycobacterium avium subsp. paratuberculosis (93 aa). Also similar to other fatty acid desaturases e.g. T35035 probable acyl-[acyl-carrier protein] desaturase from Streptomyces coelicolor (328 aa); Q40731|STAD_ORYSA acyl-[acyl-carrier protein] desaturase precursor from Oryza sativa (Rice) (390 aa); etc. Also highly similar to desA2|Rv1094 from Mycobacterium tuberculosis (275 aa). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. Belongs to the fatty acid desaturase family. Cofactor: ferredoxin, ferredoxin NADPH reductase,and NADPH. Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable acyl-[acyl-carrier protein] desaturase DesA1 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) (protein Des) 918750 desA1 885444 desA1 Mycobacterium tuberculosis H37Rv Probable acyl-[acyl-carrier protein] desaturase DesA1 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) (protein Des) YP_177758.1 917734 R 83332 CDS NP_215340.1 15607965 885354 complement(918912..919553) 1 NC_000962.3 Rv0825c, (MTV043.17c), len: 213 aa. Conserved protein, highly similar, but in part (between aa ~43-96) to fadD27|Rv0275c|MTV035.03 putative fatty-acid-CoA ligase from Mycobacterium tuberculosis (241 aa), FASTA scores: E(): 7.3e-09, (32.6% identity in 190 aa overlap). Also shows similarity with other proteins from Mycobacterium tuberculosis e.g. Rv0078|AL0214|MTV030_22 (201 aa), FASTA scores: opt:118, E(): 0.32, (34.5% identity in 113 aa overlap); etc.; hypothetical protein 919553 885354 Rv0825c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215340.1 918912 R 83332 CDS NP_215341.1 15607966 885360 919634..920689 1 NC_000962.3 Rv0826, (MTV043.18), len: 351 aa. Conserved hypothetical protein, similar to CAB94053.1|AL358672|SC7A12.06 hypothetical protein from Streptomyces coelicolor (300 aa); and NP_421372.1|NC_002696 hypothetical protein from Caulobacter crescentus (299 aa). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv1645c|Z85982|MTCY06H11.09 (351 aa),FASTA scores: opt: 1199, E(): 0, (57.5% identity in 299 aa overlap); Rv2237; Rv0276; etc.; hypothetical protein 920689 885360 Rv0826 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215341.1 919634 D 83332 CDS NP_215342.1 15607967 885375 complement(920741..921133) 1 NC_000962.3 Rv0827c, (MTV043.19c), len: 130 aa. KmtR,transcriptional regulator (See Campbell et al., 2007),similar to many e.g. CAC42856.1|AL592292 putative regulatory protein from Streptomyces coelicolor (115 aa); NP_301626.1|NC_002677 putative ArsR-family transcriptional regulator from Mycobacterium leprae (140 aa); BSUB0011_75|O31844|Z99114 YOZA protein from Bacillus subtilis (107 aa), FASTA scores: opt: 208, E(): 3.2e-08,(35.5% identity in 93 aa overlap); etc. Also similar to MTCY27.22c|Z95208 from Mycobacterium tuberculosis (135 aa),FASTA scores: opt: 201, E(): 1.2e-07, (35.7% identity in 98 aa overlap). Contains probable helix-turn helix motif from aa 42-63 (Score 1300, +3.61 SD). Belongs to the ArsR family of transcriptional regulators.; Metal sensor transcriptional regulator KmtR (ArsR-SmtB family) 921133 kmtR 885375 kmtR Mycobacterium tuberculosis H37Rv Metal sensor transcriptional regulator KmtR (ArsR-SmtB family) NP_215342.1 920741 R 83332 CDS NP_215343.1 15607968 885265 complement(921191..921613) 1 NC_000962.3 Rv0828c, (MTV043.20c), len: 140 aa. Possible deaminase, with its N-terminus highly similar to middle part of NP_302602.1|NC_002677 possible cytidine/deoxycytidylate deaminase from Mycobacterium leprae (171 aa). Also similar to other deaminases e.g. CAC18715.2|AL451182 putative deaminase from Streptomyces coelicolor (167 aa); NP_251189.1|NC_002516 probable deaminase from Pseudomonas aeruginosa (151 aa); NP_108387.1|NC_002678 nitrogen fixation protein gene from Mesorhizobium loti (149 aa); etc. Also similar to many conserved hypothetical proteins e.g. NP_389200.1|NC_000964 hypothetical protein from Bacillus subtilis (156 aa), FASTA scores: E(): 1.3e-07, (38.9% identity in 95 aa overlap); etc. And similar to Rv3752c possible deaminase from Mycobacterium tuberculosis. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family.; Possible deaminase 921613 885265 Rv0828c Mycobacterium tuberculosis H37Rv Possible deaminase NP_215343.1 921191 R 83332 CDS NP_215344.1 15607969 885403 921575..921865 1 NC_000962.3 Rv0829, (MTV043.21), len: 96 aa. Possible transposase for IS1605' (fragment), similar to C-terminal end of many mycobacterial transposases and hypothetical proteins e.g. Z74024|MTCY274_16 from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 668, E(): 6.2e-32, (98.9% identity in 93 aa overlap); MTV002_57|O33333 transposase from Mycobacterium tuberculosis ; L07627|SERRY1_1 insertion element IS1136 from Saccharopolyspora erythraea (90 aa), FASTA score: (34.9% identity in 83 aa overlap).; Possible transposase (fragment) 921865 885403 Rv0829 Mycobacterium tuberculosis H37Rv Possible transposase (fragment) NP_215344.1 921575 D 83332 CDS NP_215345.1 15607970 885886 921970..922875 1 NC_000962.3 Rv0830, (MTV043.22), len: 301 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family consisting of the proteins Rv0726c, Rv0731c, Rv3399,Rv1729c|Z81360|MTCY4C12_14c (312 aa), FASTA scores: opt: 1014, E(): 0, (54.1% identity in 292 aa overlap); etc.; Possible S-adenosylmethionine-dependent methyltransferase 922875 885886 Rv0830 Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_215345.1 921970 D 83332 CDS NP_215346.1 15607971 885349 complement(922894..923709) 1 NC_000962.3 Rv0831c, (MTV043.23c), len: 271 aa. Conserved protein, similar to Rv0347|MTY13E10_7|Z95324 conserved hypothetical protein from Mycobacterium tuberculosis (328 aa), FASTA scores: opt: 426, E(): 2.6e-21, (33.6% identity in 262 aa overlap).; hypothetical protein 923709 885349 Rv0831c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215346.1 922894 R 83332 CDS YP_177759.1 57116787 885236 924951..925364 1 NC_000962.3 Rv0832, (MTV043.24), len: 137 aa. PE_PGRS12, Member of the Mycobacterium tuberculosis PE family, possibly PGRS subfamily of gly-rich proteins (see citation below), highly similar to many others e.g. MTCY1A11.25c|Z78020 (498 aa),FASTA scores: opt: 529, E(): 5.2e-22, (61.8% identity in 136 aa overlap); etc. Appears to have incurred frameshift as next ORF should be continuation; sequence has been checked but no error found.; PE-PGRS family protein PE_PGRS12 925364 PE_PGRS12 885236 PE_PGRS12 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS12 YP_177759.1 924951 D 83332 CDS YP_177760.1 57116788 885391 925361..927610 1 NC_000962.3 Rv0833, (MTV043.25), len: 749 aa. PE_PGRS13, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), but lacking N-terminal domain (present in preceding ORF),possibly due to frameshift. Similar in part to many others e.g. MTCY28_25|Z95890 (914 aa), FASTA scores: opt: 2726,E(): 0, (60.1% identity in 776 aa overlap); etc.; PE-PGRS family protein PE_PGRS13 927610 PE_PGRS13 885391 PE_PGRS13 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS13 YP_177760.1 925361 D 83332 CDS YP_177761.1 57116789 885369 complement(927837..930485) 1 NC_000962.3 Rv0834c, (MTV043.26c), len: 882 aa. PE_PGRS14,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002),highly similar to many others e.g. MTCY493_4|Z95844 (1329 aa), FASTA scores: opt: 2577, E(): 0, (52.0% identity in 950 aa overlap); etc. Thought to be differentially expressed within host cells (see Triccas et al., 1999).; PE-PGRS family protein PE_PGRS14 930485 PE_PGRS14 885369 PE_PGRS14 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS14 YP_177761.1 927837 R 83332 CDS NP_215350.1 15607975 885883 930953..931597 1 NC_000962.3 Rv0835, (MTV043.27), len: 214 aa. Possible lpqQ,lipoprotein. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Possible lipoprotein LpqQ 931597 lpqQ 885883 lpqQ Mycobacterium tuberculosis H37Rv Possible lipoprotein LpqQ NP_215350.1 930953 D 83332 CDS NP_215351.1 15607976 885356 complement(932279..932932) 1 NC_000962.3 Rv0836c, (MTV043.29c), len: 217 aa (start uncertain). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 932932 885356 Rv0836c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215351.1 932279 R 83332 CDS NP_215352.1 15607977 885109 complement(933003..934031) 1 NC_000962.3 Rv0837c, (MTV043.30c), len: 342 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 934031 885109 Rv0837c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215352.1 933003 R 83332 CDS NP_215353.1 15607978 885417 934720..935490 1 NC_000962.3 Rv0838, (MTV043.31), len: 256 aa. Probable lpqR,conserved lipoprotein. Similar (except in N-terminus) to hypothetical proteins and D-alanyl-D-alanine dipeptidases e.g. NP_416005.1|NC_000913 hypothetical protein from Escherichia coli strain K12 (193 aa); NP_421076.1|NC_002696 D-alanyl-D-alanine dipeptidase from Caulobacter crescentus (212 aa); Q06241|VANX_ENTFC D-alanyl-D-alanine dipeptidase from Enterococcus faecium (202 aa), FASTA scores: opt: 198,E(): 1.9e-05, (28.1% identity in 199 aa overlap); etc. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LpqR 935490 lpqR 885417 lpqR Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqR NP_215353.1 934720 D 83332 CDS NP_215354.1 15607979 885255 935577..936389 1 NC_000962.3 Rv0839, (MTV043.32), len: 270 aa. Conserved hypothetical protein, similar to various hypothetical proteins or methyltransferases from yeast and bacteria e.g. T34740|SC1E6.19c|AL033505|SC1E6_19 hypothetical protein from Streptomyces coelicolor (273 aa), FASTA scores: opt: 1102, E(): 0, (58.6% identity in 263 aa overlap); T38024|Z98598|SPAC1B3.06c hypothetical protein from Schizosaccharomyces pombe (278 aa), FASTA scores: opt: 562,E(): 1.9e-3, (36.4% identity in 269 aa overlap); JC6531 avermectin B 5-O-methyltransferase from Streptomyces avermitilis (283 aa); etc. Also similar to other Mycobacterium tuberculosis hypothetical proteins that may be methyltransferases e.g. Rv1523, Rv2952, Rv1405c, etc.; hypothetical protein 936389 885255 Rv0839 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215354.1 935577 D 83332 CDS NP_215355.1 15607980 885611 complement(936457..937317) 1 NC_000962.3 Rv0840c, (MTV043.33c), len: 286 aa. Possible pip,proline iminopeptidase, similar to many e.g. P46541|PIP_BACCO proline iminopeptidase from bacillus coagulans (288 aa), FASTA scores: opt: 657, E(): 0, (37.6% identity in 282 aa overlap); NP_386922.1|NC_003047 putative proline iminopeptidase protein from Sinorhizobium meliloti (296 aa); etc. Belongs to peptidase family S33.; Probable proline iminopeptidase Pip (prolyl aminopeptidase) (pap) 937317 pip 885611 pip Mycobacterium tuberculosis H37Rv Probable proline iminopeptidase Pip (prolyl aminopeptidase) (pap) NP_215355.1 936457 R 83332 CDS YP_177634.1 57116790 3205068 937593..937835 1 NC_000962.3 Rv0841, len: 80 aa. Conserved transmembrane protein,highly similar to C-terminus of next ORF Rv0842|O53854 putative membrane protein from Mycobacterium tuberculosis (442 aa), FASTA scores: opt: 246, E(): 3.3e-10, (59.7% identity in 72 aa overlap). Replace previous Rv0841c.; Probable conserved transmembrane protein 937835 3205068 Rv0841 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein YP_177634.1 937593 D 83332 CDS NP_215357.1 15607982 885616 938112..939404 1 NC_000962.3 Rv0842, (MT0864, MTV043.35), len: 430 aa. Probable conserved integral membrane protein, showing similarity with other integral membrane proteins e.g. P28246|BCR_ECOLI bicyclomycin resistance protein from EScherichia coli (396 aa), FASTA scores: opt: 216, E(): 5.4e-07, (23.7% identity in 376 aa overlap); etc.; Probable conserved integral membrane protein 939404 885616 Rv0842 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215357.1 938112 D 83332 CDS NP_215358.1 15607983 885554 939388..940392 1 NC_000962.3 Rv0843, (MTV043.36), len: 334 aa. Probable dehydrogenase, similar to various dehydrogenases e.g. Q46142|Q46142 TPP-dependent acetoin dehydrogenase (326 aa),FASTA scores: opt: 500, E(): 2.4e-26, (32.3% identity in 300 aa overlap); P51267|ODPA_PORPU pyruvate dehydrogenase E1 component from Porphyra purpurea (344 aa), FASTA scores: opt: 451, E(): 4.7e-23, (29.6% identity in 311 aa overlap); etc. Also similar to Rv2497c|pdhA pyruvate dehydrogenase E1 component from Mycobacterium tuberculosis (367 aa).; Probable dehydrogenase 940392 885554 Rv0843 Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_215358.1 939388 D 83332 CDS NP_215359.1 15607984 885603 complement(940456..941106) 1 NC_000962.3 Rv0844c, (MTV043.37c), len: 216 aa. Possible narL,nitrate/nitrite response regulator protein, similar to many e.g. CAB44989.1|AJ131854 NarL protein from Pseudomonas stutzeri (218 aa); CAA75536.1|Y15252 nitrate/nitrite regulatory protein from Pseudomonas aeruginosa (216 aa); PCC6803|D64005|SYCSLRG_24 NarL protein from Synechocystis sp. (209 aa), FASTA scores: opt: 438, E(): 1.5e-23, (34.6% identity in 208 aa overlap); etc. Also similar to unidentified regulator e.g. CAB76009.1|AL157916 putative two-component system response regulator from Streptomyces coelicolor (224 aa); etc. Contains probable helix-turn helix motif from aa 170-191 (Score 1124, +3.02 SD).; Possible nitrate/nitrite response transcriptional regulatory protein NarL 941106 narL 885603 narL Mycobacterium tuberculosis H37Rv Possible nitrate/nitrite response transcriptional regulatory protein NarL NP_215359.1 940456 R 83332 CDS NP_215360.1 15607985 885218 941190..942467 1 NC_000962.3 Rv0845, (MTV043.38), len: 425 aa. Possible two-component sensor kinase, with its C-terminus similar to C-terminal part of others e.g. NP_294951.1|NC_001263 two-component sensor histidine kinase from Deinococcus radiodurans (469 aa); CAC32293.1|AL583943 putative two component system histidine kinase from Streptomyces coelicolor (404 aa); NP_464546.1|NC_003210 protein similar to two-component sensor histidine kinase from Listeria monocytogenes (352 aa); BSUB0017_193|Z9912 two-component sensor kinase from Bacillus subtilis (360 aa), FASTA scores: opt: 275, E(): 1.6e-11, (30.3% identity in 234 aa overlap); etc.; Possible two component sensor kinase 942467 885218 Rv0845 Mycobacterium tuberculosis H37Rv Possible two component sensor kinase NP_215360.1 941190 D 83332 CDS NP_215361.1 15607986 885207 complement(942680..944194) 1 NC_000962.3 Rv0846c, (MTV043.39c), len: 504 aa. Probable oxidase, showing similarity with several oxidases, mainly L-ascorbate oxidases and copper resistance proteins a (precursors) e.g. P24792|ASO_CUCMA L-ascorbate oxidase precursor (ascorbase) from Cucurbita maxima (Pumpkin) (Winter squash) (579 aa), FASTA scores: opt: 423, E(): 5.8e-18, (28.4% identity in 493 aa overlap); AF010496|AF010496_32 potential multicopper oxidase from Rhodobacter capsulatus (491 aa), FASTA scores: opt: 490,E(): 2.7e-22, (28.8% identity in 510 aa overlap); 47452|PCOA_ECOLI copper resistance protein A precursor (belongs to the family of multicopper oxidases) from Escherichia coli strain K12 (605 aa); etc. Contains PS00080 Multicopper oxidases signature 2 at C-terminus. Seems to belong to the family of multicopper oxidases.; Probable oxidase 944194 885207 Rv0846c Mycobacterium tuberculosis H37Rv Probable oxidase NP_215361.1 942680 R 83332 CDS NP_215362.1 15607987 885051 944343..944735 1 NC_000962.3 Rv0847, (MTV043.40), len: 130 aa. Probable lpqS,lipoprotein. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LpqS 944735 lpqS 885051 lpqS Mycobacterium tuberculosis H37Rv Probable lipoprotein LpqS NP_215362.1 944343 D 83332 CDS YP_177762.1 57116791 885545 944938..946056 1 NC_000962.3 Rv0848, (MTV043.41), len: 372 aa. Possible cysK2,cysteine synthase A, but could be also a cysteine synthase B cysM2-product, similar to many e.g. NP_109408.1|NC_002682 cysteine synthase from Mesorhizobium loti (357 aa); Q44004|CYSM_ALCEU cysteine synthase from Alcaligenes eutrophus strain CH34 (Ralstonia eutropha) (339 aa), FASTA scores: opt: 511, E(): 1.7e-25, (35.0% identity in 314 aa overlap); etc. Belongs to the cysteine synthase/cystathionine beta-synthase family. Cofactor: pyridoxal phosphate. Note that previously known as cysM3.; Possible cysteine synthase a CysK2 (O-acetylserine sulfhydrylase) (O-acetylserine (thiol)-lyase) (CSASE) 946056 cysK2 885545 cysK2 Mycobacterium tuberculosis H37Rv Possible cysteine synthase a CysK2 (O-acetylserine sulfhydrylase) (O-acetylserine (thiol)-lyase) (CSASE) YP_177762.1 944938 D 83332 CDS NP_215364.1 15607989 885111 946056..947315 1 NC_000962.3 Rv0849, (MTV043.42), len: 419 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS) involved in transport of drug, showing similarity with others e.g. T35055 probable transport system permease protein from Streptomyces coelicolor (436 aa); NP_295031.1|NC_001263 major facilitator family protein from Deinococcus radiodurans (458 aa); NP_455659.1|NC_003198 putative membrane transporter from Salmonella enterica subsp. enterica serovar Typhi (402 aa); etc.; Probable conserved integral membrane transport protein 947315 885111 Rv0849 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein NP_215364.1 946056 D 83332 CDS NP_215365.1 15607990 885054 947312..947644 1 NC_000962.3 Rv0850, (MTV043.43), len: 110 aa. Putative transposase (fragment), similar in part to others e.g. Q45144|Q4514 transposable element IS31831 (436 aa), FASTA scores: opt: 175, E(): 4.3e-05, (38.6% identity in 57 aa overlap); etc.; Putative transposase (fragment) 947644 885054 Rv0850 Mycobacterium tuberculosis H37Rv Putative transposase (fragment) NP_215365.1 947312 D 83332 CDS NP_215366.1 15607991 885550 complement(947641..948468) 1 NC_000962.3 Rv0851c, (MTV043.44c), len: 275 aa. Probable short-chain dehydrogenase/reductase, similar to many e.g. Q01198|LIGD_PSEPA C alpha-dehydrogenase (SDR family) from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (305 aa); D11473|PSELIG_1 C alpha-dehydrogenase from P. paucimobilis (305 aa), FASTA scores: opt: 468, E(): 4.9e-23, (30.8% identity in 279 aa overlap); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 948468 885550 Rv0851c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_215366.1 947641 R 83332 CDS NP_215367.1 15607992 885044 948559..949395 1 NC_000962.3 Rv0852, (MTV043.45), len: 278 aa. Possible fadD16,fatty-acid-CoA synthetase, similar in part to various CoA ligases e.g. P18163|LCFB_RAT long-chain-fatty-acid--CoA ligase from Rattus norvegicus (Rat) (699 aa); D49366|LEP4CCOALA_1 4-coumarate:CoA ligase from Lithospermum erythrorhizon (636 aa), FASTA scores: opt: 134, E(): 0.15, (26.8% identity in 213 aa overlap); orgp|L09229|HUMFACAL_1 long-chain acyl-coenzyme A from homo sapiens (human) (699 aa), FASTA score: (50.0% identity in 40 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; Possible fatty-acid-CoA ligase FadD16 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 949395 fadD16 885044 fadD16 Mycobacterium tuberculosis H37Rv Possible fatty-acid-CoA ligase FadD16 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_215367.1 948559 D 83332 CDS NP_215368.1 15607993 885576 complement(949436..951118) 1 NC_000962.3 Rv0853c, (MTV043.46c), len: 560 aa. Probable pdc,pyruvate or indole-pyruvate decarboxylase, equivalent to NP_302424.1|NC_002677 pyruvate (or indolepyruvate) decarboxylase from Mycobacterium leprae (569 aa). Also highly similar to others e.g. AAB06571.1|L80006 indolepyruvate decarboxylase from Pantoea agglomerans (550 aa); Q12629|DCPY_KLULA pyruvate decarboxylase from Kluyveromyces marxianus var. lactis (563 aa); P71323 indolepyruvate decarboxylase from Enterobacter herbicola (550 aa), FASTA scores: opt: 1642, E(): 0, (48.1% identity in 547 aa overlap); P23234|DCIP_ENTCL indole-3-pyruvate decarboxylase (indolepyruvate decarboxylase) from Enterobacter cloacae (552 aa), FASTA scores: opt: 1596,E(): 0, (46.8% identity in 551 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature and PS00017 ATP/GTP-binding site motif A (P-loop). Cofactor: thiamine pyrophosphate.; Probable pyruvate or indole-3-pyruvate decarboxylase Pdc 951118 pdc 885576 pdc Mycobacterium tuberculosis H37Rv Probable pyruvate or indole-3-pyruvate decarboxylase Pdc NP_215368.1 949436 R 83332 CDS NP_215369.1 15607994 885127 951183..951626 1 NC_000962.3 Rv0854, (MTV043.47), len: 147 aa. Conserved protein,similar to several hypothetical protein from Mycobacterium leprae e.g. NP_301674.1|NC_002677 (144 aa); NP_302683.1|NC_002677|Z95398|MLCL622.27c (156 aa), FASTA scores: opt: 193, E(): 1.6e-06, (24.6% identity in 134 aa overlap); NP_301218.1|NC_002677 (146 aa); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 171, E(): 5.8e-05, (21.5% identity in 135 aa overlap). Also similar to SC6G10.02c|T35511|AL049497|SC6G10_2 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 344, E(): 6.1e- 17, (37.6% identity in 141 aa overlap). And similar to many proteins from Mycobacterium tuberculosis e.g. downstreams ORFs Rv0856 and Rv0857, etc.; hypothetical protein 951626 885127 Rv0854 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215369.1 951183 D 83332 CDS NP_215370.1 15607995 885790 951632..952711 1 NC_000962.3 Rv0855, (MTV043.48), len: 359 aa. Probable far,fatty acid-CoA racemase, highly similar to CAB08122.1|Z94723 unknown protein from Mycobacterium leprae (253 aa) (C-terminus shorter). Also similar to many eukaryotic and bacteria racemases e.g. T35425 probable fatty acid CoA racemase from Streptomyces coelicolor (387 aa); P70473|AMAC_RAT alpha-methylacyl-CoA racemase (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) from Rattus norvegicus (Rat) (382 aa); NP_103687.1|NC_002678 probable fatty acid Co-a racemase from Mesorhizobium loti (389 aa); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Rv1143|MTCI65.10|MCR from Mycobacterium tuberculosis (360 aa), FASTA scores: opt: 1373, E(): 0, (56.8% identity in 359 aa overlap), Rv1866|MTCY359.07 (C-terminal half) (778 aa), Rv3272 (360 aa).; Probable fatty-acid-CoA racemase Far 952711 far 885790 far Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA racemase Far NP_215370.1 951632 D 83332 CDS NP_215371.1 15607996 885783 952825..953229 1 NC_000962.3 Rv0856, (MTV043.49), len: 134 aa. Conserved hypothetical protein, showing weak similarity with NP_301674.1| (NC_002677) conserved hypothetical protein from Mycobacterium leprae (144 aa); and SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. neighbouring ORF downstream Rv0857 conserved hypothetical protein (126 aa), FASTA scores: E(): 7.4e-27, (62.0% identity in 100 aa overlap); neighbouring ORF Rv0854|MTV043_47 conserved hypothetical protein (147 aa), FASTA scores: E(): 1.6e-15, (36.6% identity in 123 aa overlap), MTCI28.04|Z97050|MTCI28_4 (184 aa), FASTA scores: opt: 127, E(): 0.036, (26.0% identity in 127 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 123,E(): 0.06, (26.4% identity in 125 aa overlap).; hypothetical protein 953229 885783 Rv0856 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215371.1 952825 D 83332 CDS NP_215372.2 57116792 885078 953257..953730 1 NC_000962.3 Rv0857, (MTV043.50), len: 157 aa. Conserved hypothetical protein, showing weak similarity with Q9X7Y8|SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 215,E(): 7.6e-08, (30.282% identity in 142 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. upstream ORF Rv0856 (134 aa), FASTA scores: opt: 566, E(): 2e-32, (58.15% identity in 129 aa overlap); upstream ORF Rv0854 (147 aa), FASTA scores: opt: 401, E(): 7.2e-21, (41.8% identity in 146 aa overlap); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 122, E(): 0.031, (29.4% identity in 85 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 114, E(): 0.1, (30.9% identity in 55 aa overlap). Length extended since first submission (+33 aa).; hypothetical protein 953730 885078 Rv0857 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215372.2 953257 D 83332 CDS NP_215373.1 15607998 885784 complement(953727..954920) 1 NC_000962.3 Rv0858c, (MTV043.51c), len: 397 aa. Probable dapC,N-succinyldiaminopimelate aminotransferase, highly similar to others from Eukaryota and bacteria, especially aspartate aminotransferases (transaminases), e.g. NP_177890.1|NC_003070 putative aminotransferase from Arabidopsis thaliana (440 aa); NP_419555.1|NC_002696 aminotransferase class I from Caulobacter crescentus (385 aa); NP_415133.1|NC_000913|AE0001|ECAE000165_8 putative aminotransferase from Escherichia coli strain K12 (386 aa),FASTA scores: opt: 830, E(): 0, (38.0% identity in 389 aa overlap); X99521|TAX99521_1 aspartate aminotransferase from Thermus aquaticus (383 aa), FASTA scores: opt: 702, E(): 0,(34.9% identity in 393 aa overlap); etc. Also similar to other putative aminotransferases from Mycobacterium tuberculosis e.g. Rv2294, Rv3565, etc.; Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at) 954920 dapC 885784 dapC Mycobacterium tuberculosis H37Rv Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at) NP_215373.1 953727 R 83332 CDS NP_215374.1 15607999 885774 955077..956288 1 NC_000962.3 Rv0859, (MTV043.52), len: 403 aa. Possible fadA,acyl-CoA thiolase, equivalent to NP_302423.1|NC_002677 putative beta-ketoadipyl CoA thiolase from Mycobacterium leprae (403 aa). Also highly similar to acyl/acetyl-CoA thiolases and beta-ketoadipyl CoA thiolases, e.g. T35428 probable acetyl CoA acetyltransferase (thiolase) from Streptomyces coelicolor (404 aa); NP_250427.1|NC_002516 probable acyl-CoA thiolase from Pseudomonas aeruginosa (401 aa); NP_106253.1|NC_002678 probable acyl-CoA thiolase from Mesorhizobium loti (402 aa); NP_248919.1|NC_002516|PcaF beta-ketoadipyl CoA thiolase PcaF from Pseudomonas aeruginosa (401 aa); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2 and PS00099 Thiolases active site.; Possible acyl-CoA thiolase FadA 956288 fadA 885774 fadA Mycobacterium tuberculosis H37Rv Possible acyl-CoA thiolase FadA NP_215374.1 955077 D 83332 CDS NP_215375.1 15608000 885799 956293..958455 1 NC_000962.3 Rv0860, (MTV043.53), len: 720 aa. Probable fadB,fatty oxidation protein, equivalent to NP_302422.1|NC_002677 putative fatty oxidation complex alpha subunit from Mycobacterium leprae (714 aa). Also highly similar to others and various proteins involved in fatty acid metabolism, e.g. T35429 probable fatty oxidation protein from Streptomyces coelicolor (733 aa); NP_250428.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (714 aa); NP_418895.1|NC_002696 fatty oxidation complex alpha subunit from Caulobacter crescentus (709 aa); P40939|ECHA_HUMAN trifunctional enzyme alpha subunit [includes: long-chain enoyl-CoA hydratase ; long chain 3-hydroxyacyl-CoA dehydrogenase ] from Homo sapiens (763 aa), FASTA scores: opt: 1176, E(): 0, (32.4% identity in 722 aa overlap); P21177|FADB_ECOLI fatty oxidation complex alpha subunit [includes: enoyl-CoA hydratase; delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; 3- hydroxybutyryl-CoA epimerase] from Escherichia coli strain K12 (729 aa), FASTA scores: opt: 873, E(): 0, (33.6% identity in 693 aa overlap); etc.; Probable fatty oxidation protein FadB 958455 fadB 885799 fadB Mycobacterium tuberculosis H37Rv Probable fatty oxidation protein FadB NP_215375.1 956293 D 83332 CDS NP_215376.3 448824750 885425 complement(958523..960151) 1 NC_000962.3 Rv0861c, (MTV043.54c), len: 542 aa. Ercc3, DNA helicase (see citation below), equivalent to NP_302420.1|NC_002677 probable DNA helicase from Mycobacterium leprae (549 aa). Also highly similar to others (shorter than several eukaryotic enzymes) e.g. NP_218820.1|NC_000919|AE001217|AE0 01217_6 putative DNA repair helicase from Treponema pallidum (606 aa), FASTA scores: opt: 1275, E(): 0, (47.5% identity in 592 aa overlap); Q00578|RA25_YEAST DNA repair helicase from Saccharomyces cerevisiae (843 aa), FASTA scores: opt: 777,E(): 0, (30.4% identity in 605 aa overlap); P49135|XPB_MOUSE DNA-repair protein complementing XP-B cells from Mus musculus (Mouse) (783 aa), FASTA scores: opt: 761, E(): 0, (36.3% identity in 375 aa overlap); etc. Seems to belong to the helicase family. Alternative nucleotide at position 958922 (C->a; A410A) has been observed.; DNA helicase Ercc3 960151 ercc3 885425 ercc3 Mycobacterium tuberculosis H37Rv DNA helicase Ercc3 NP_215376.3 958523 R 83332 CDS NP_215377.1 15608002 885413 complement(960342..962612) 1 NC_000962.3 Rv0862c, (MTV043.55c), len: 756 aa. Conserved protein, equivalent to NP_302419.1|NC_002677 possible DNA-binding protein from Mycobacterium leprae (753 aa); and highly similar (except in C-terminus) to MLCB57.01|Z99494|T45333 hypothetical protein from Mycobacterium leprae (>577 aa, truncated), FASTA scores: opt: 3047, E(): 0, (78.9% identity in 578 aa overlap). Also similar in part to SCD12A.03c|AB93395.1|AL357524 hypothetical protein from Streptomyces coelicolor (867 aa).; hypothetical protein 962612 885413 Rv0862c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215377.1 960342 R 83332 CDS NP_215378.1 15608003 885423 962599..962880 1 NC_000962.3 Rv0863, (MTV043.56), len: 93 aa. Conserved hypothetical protein, highly similar to NP_302418.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (74 aa). Also weakly similar in part to U82598|ECU82598_135 hypothetical protein from Escherichia coli, FASTA scores: (32.4% identity in 71 aa overlap); and M74011|YEPYSCOP_8 hypothetical protein from Yersinia enterocolitica (165 aa), FASTA scores: (38.6 identity in 57 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 962880 885423 Rv0863 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215378.1 962599 D 83332 CDS NP_215379.1 15608004 885826 962890..963393 1 NC_000962.3 Rv0864, (MTV043.57), len: 167 aa. Probable moaC2,molybdopterin cofactor biosynthesis protein, highly similar to others e.g. CAB59676.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (170 aa); NP_418834.1|NC_002696 molybdenum cofactor biosynthesis protein C from Caulobacter crescentus (186 aa); Y10817|ANY10817_3|T44852 molybdopterin co-factor synthesis protein moaC from Arthrobacter nicotinovorans plasmid pAO1 (169 aa), FASTA scores: opt: 491, E(): 2.4e-29, (51.0% identity in 151 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 491, E(): 2.4e-29, (54.9% identity in 153 aa overlap); and O53376|Rv3324c|MOAC3|MTV016.24c putative molybdenum cofactor biosynthesis protein C3 (177 aa).; Probable molybdenum cofactor biosynthesis protein C 2 MoaC2 963393 moaC2 885826 moaC2 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein C 2 MoaC2 NP_215379.1 962890 D 83332 CDS NP_215380.1 15608005 885348 963390..963872 1 NC_000962.3 Rv0865, (MTV043.58), len: 160 aa. Probable mog,molybdopterin biosynthesis MOG protein, highly similar or similar to other molybdenum cofactor biosynthesis proteins e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa); CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa); P44645|MOG_HAEIN|MOGA|HI0336 molybdopterin biosynthesis MOG protein from Haemophilus influenzae (197 aa), FASTA scores: opt: 306, E(): 9e-13,(39.6% identity in 139 aa overlap); P28694|MOG_ECOLI molybdopterin biosynthesis MOG protein from Escherichia coli (195 aa), FASTA scores: opt: 265, E(): 3.6e-10, (34.2 identity in 146 aa overlap); etc. Also highly similar to Rv0984|MTV044.12|MOAB2 possible pterin-4-alpha-carbinolamine dehydratase from Mycobacterium tuberculosis (181 aa).; Probable molybdopterin biosynthesis Mog protein 963872 mog 885348 mog Mycobacterium tuberculosis H37Rv Probable molybdopterin biosynthesis Mog protein NP_215380.1 963390 D 83332 CDS NP_215381.1 15608006 885431 963869..964294 1 NC_000962.3 Rv0866, (MTV043.59), len: 141 aa. Probable moaE2,molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), similar to others e.g. Y10817|ANY10817_4|T44853 molybdopterin biosynthesis protein E chain from Arthrobacter nicotinovorans plasmid pAO1 (155 aa), FASTA scores: opt: 460, E(): 3.5e-27, (49.3 identity in 146 aa overlap); CAC01331.1|AL390968 moaE-like protein from Streptomyces coelicolor (152 aa); NP_389313.1|NC_000964 molybdopterin converting factor (subunit 2) from Bacillus subtilis (157 aa); etc. Also highly similar to Rv3119|MOAE1|Z95150|MTCY164_30 putative molybdenum cofactor biosynthesis protein E from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 321, E(): 5.9e-17, (40.9% identity in 132 aa overlap); and O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa).; Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) 964294 moaE2 885431 moaE2 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) NP_215381.1 963869 D 83332 CDS NP_215382.1 15608007 885749 complement(964312..965535) 1 NC_000962.3 Rv0867c, (MTV043.60c), len: 407 aa. Possible rpfA,resuscitation-promoting factor (see citation below). N-terminus highly similar to N-terminal part (1-125 aa) of Z99494|MLCB57_3|NP_302417.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (174 aa),FASTA scores: opt: 785, E(): 1.8e-18, (63.0% identity in 200 aa overlap); and highly similar to C-terminus of NP_301299.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (375 aa); and middle part of NP_302360.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (157 aa). N-terminus also highly similar in part of three secreted proteins from Streptomyces coelicolor e.g. CAC09538.1|AL442120 putative secreted protein (244 aa). Regions highly similar to CAB76321.1|AL158060 putative membrane protein from Streptomyces coelicolor (121 aa); and middle part of CAB09664.1|Z96935 rpf from Micrococcus luteus (220 aa). Also highly similar in part to four resuscitation-promoting factors from Mycobacterium tuberculosis: Rv2450 (172 aa),Rv1009 (362 aa), Rv1884c (176 aa), and Rv2389c (154 aa). Contains a probable secretory signal sequence in N-terminus. Predicted possible vaccine candidate (See Zvi et al., 2008).; Possible resuscitation-promoting factor RpfA 965535 rpfA 885749 rpfA Mycobacterium tuberculosis H37Rv Possible resuscitation-promoting factor RpfA NP_215382.1 964312 R 83332 CDS NP_215383.1 15608008 885763 complement(965983..966261) 1 NC_000962.3 Rv0868c, (MTV043.61c), len: 92 aa. Probable moaD2,molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to CAB88494.1|AL353816 putative molybdopterin converting factor from Streptomyces coelicolor (84 aa); and weakly similar to others MoaD proteins e.g. Z99111|BSUB0008_103 from Bacillus subtilis (77 aa), FASTA scores: opt: 86, E(): 2.8, (22.9% identity in 83 aa overlap); etc. Also some similarity with Rv3112|MOAD1|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 113, E(): 0.024,(31.3% identity in 83 aa overlap).; Probable molybdenum cofactor biosynthesis protein D 2 MoaD2 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) 966261 moaD2 885763 moaD2 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein D 2 MoaD2 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) NP_215383.1 965983 R 83332 CDS NP_215384.1 15608009 885773 complement(966265..967347) 1 NC_000962.3 Rv0869c, (MTV043.62c), len: 360 aa. Probable moaA2,molybdenum cofactor biosynthesis protein, highly similar to others e.g. CAB59437.1|AL132644|SCI8_6 molybdenum cofactor biosynthesis protein A from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1336, E(): 0, (61.7% identity in 332 aa overlap); S57490|X78980|ANMOAA_1 molybdopterin cofactor synthesis protein from Arthrobacter nicotinovorans (fragment) (374 aa), FASTA scores: opt: 1059, E(): 0,(49.9% identity in 369 aa overlap); Q44118|MOAA_ARTNI probable molybdopterin cofactor synthesis protein A from Arthrobacter nicotinovorans plasmid pAO1 (355 aa); etc. Also similar to Rv3109|MTCY164.19|Z95150|MOAA1 putative molybdenum cofactor biosynthesis protein A from Mycobacterium tuberculosis (359 aa), FASTA scores: opt: 657, E(): 0, (36.6% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family.; Probable molybdenum cofactor biosynthesis protein A2 MoaA2 967347 moaA2 885773 moaA2 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein A2 MoaA2 NP_215384.1 966265 R 83332 CDS NP_215385.1 15608010 885709 complement(967344..967733) 1 NC_000962.3 Rv0870c, (MTV043.63c), len: 129 aa. Possible conserved integral membrane protein, highly similar to other membrane proteins: putative secreted proteins or hypothetical proteins e.g. CAC08263.1| AL392146 putative integral membrane protein from Streptomyces coelicolor (138 aa); NP_233433.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (143 aa); NP_455572.1|NC_003198 putative membrane protein from Salmonella enterica subsp. enterica serovar Typhi (148 aa); P37065|YCCF_ECOLI hypothetical 16.3 kDa protein from Escherichia coli (148 aa), FASTA scores: opt: 183, E(): 1.9e-06, (36.6% identity in 134 aa overlap); etc.; Possible conserved integral membrane protein 967733 885709 Rv0870c Mycobacterium tuberculosis H37Rv Possible conserved integral membrane protein NP_215385.1 967344 R 83332 CDS NP_215386.1 15608011 885725 967898..968305 1 NC_000962.3 Rv0871, (MTV043.64), len: 135 aa. Probable cspB,cold shock-like protein B, equivalent to Z99494|MLCB57_7|MLCB57.11 probable cold shock protein from Mycobacterium leprae (136 aa), FASTA scores: opt: 787, E(): 0, (86.0% identity in 136 aa overlap). Also highly similar (but often longer than) to others e.g. CAB93399.1|AL357524 cold shock protein B from Streptomyces coelicolor (127 aa); Q45099|CSPD_BACCE cold shock-like protein CSPD from Bacillus cereus (66 aa); Y101 81|LLCSPB_1 cold shock protein from Lactococcus lactis (66 aa), FASTA scores: opt: 220, E(): 2.5e-07, (48.3% identity in 60 aa overlap); etc. Seems to belong to the cold-shock domain (CSD) family.; Probable cold shock-like protein B CspB 968305 cspB 885725 cspB Mycobacterium tuberculosis H37Rv Probable cold shock-like protein B CspB NP_215386.1 967898 D 83332 CDS YP_177763.1 57116793 885742 complement(968424..970244) 1 NC_000962.3 Rv0872c, (MTV043.65c), len: 606 aa. PE_PGRS15,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002),similar to many e.g. MTCY24A1.04c|Z95207 (615 aa), FASTA scores: opt: 2636, E(): 0, (64.6% identity in 619 aa overlap); etc.; PE-PGRS family protein PE_PGRS15 970244 PE_PGRS15 885742 PE_PGRS15 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS15 YP_177763.1 968424 R 83332 CDS NP_215388.1 15608013 885636 970505..972457 1 NC_000962.3 Rv0873, (MTV043.66-MTCY31.01), len: 650 aa. Probable fadE10, acyl-CoA dehydrogenase, highly similar to many e.g. CAB91129.1|AL355913 putative acyl CoA dehydrogenase from Streptomyces coelicolor (658 aa); P50544|ACDV_MOUSE acyl-CoA dehydrogenase from Mus musculus (656 aa); D30647|RATVLCAD_1 very-long-chain Acyl-CoA dehydrogenase from Rattus norvegicus (655 aa), FASTA scores: opt: 675,E(): 0, (33.9% identity in 380 aa overlap); etc.; Probable acyl-CoA dehydrogenase FadE10 972457 fadE10 885636 fadE10 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE10 NP_215388.1 970505 D 83332 CDS NP_215389.1 15608014 885658 complement(972546..973706) 1 NC_000962.3 Rv0874c, (MTCY31.02c), len: 386 aa. Conserved hypothetical protein, highly similar in part to SPU62616_1 hypothetical protein from Synechococcus sp. (280 aa), FASTA scores: E(): 6.3e-26, (35.2% identity in 264 aa overlap); SYCSLLLH_102 from Synechocystis sp. (447 aa), FASTA scores: E(): 1.1e-18, (29.5% identity in 400 aa overlap). Also highly similar to Rv0628c|MTCY20H10_9 from Mycobacterium tuberculosis (383 aa), FASTA scores: E():0, (81.5% identity in 383 aa overlap).; hypothetical protein 973706 885658 Rv0874c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215389.1 972546 R 83332 CDS NP_215390.1 15608015 885669 complement(973806..974294) 1 NC_000962.3 Rv0875c, (MTCY31.03c), len: 162 aa. Possible conserved exported protein, equivalent to MLCB57_11|O33056 possible exported protein from Mycobacterium leprae (162 aa), FASTA scores: opt: 789, E(): 0, (71.4% identity in 161 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al.,2008).; Possible conserved exported protein 974294 885669 Rv0875c Mycobacterium tuberculosis H37Rv Possible conserved exported protein NP_215390.1 973806 R 83332 CDS NP_215391.1 15608016 885562 complement(974291..975937) 1 NC_000962.3 Rv0876c, (MTCY31.04c), len: 548 aa. Possible conserved transmembrane protein, equivalent to MLCB57_12|O33057 possible membrane protein from Mycobacterium leprae (579 aa), FASTA scores: opt: 2850,E(): 0, (81.0% identity in 568 aa overlap). Also highly similar (except in N-terminus) to CAB93403.1|AL357524 putative integral membrane protein from Streptomyces coelicolor (463 aa).; Possible conserved transmembrane protein 975937 885562 Rv0876c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_215391.1 974291 R 83332 CDS NP_215392.1 15608017 885601 976075..976863 1 NC_000962.3 Rv0877, (MTCY31.05), len: 262 aa. Conserved hypothetical protein, equivalent to MLCB57_13|O33058 conserved hypothetical protein from Mycobacterium leprae (269 aa), FASTA scores: E(): 0, (80.5% identity in 257 aa overlap). Also highly similar (except in C-terminus) to SCD12A.13|CAB93404.1|AL357524 hypothetical protein from Streptomyces coelicolor (308 aa).; hypothetical protein 976863 885601 Rv0877 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215392.1 976075 D 83332 CDS YP_177764.1 57116794 885617 complement(976872..978203) 1 NC_000962.3 Rv0878c, (MTCY31.06c), len: 443 aa. PPE13, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. P4261|YHS6_MYCTU (517 aa), FASTA scores: opt: 1044, E(): 0, (47.4% identity in 397 aa overlap); MTV014_3,MTCI65_2, MTCY98_24, MTCY3C7_23, MTCY48_17, MTV004_5,MTV004_3, etc. Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE13 978203 PPE13 885617 PPE13 Mycobacterium tuberculosis H37Rv PPE family protein PPE13 YP_177764.1 976872 R 83332 CDS NP_215394.1 15608019 885095 complement(978481..978756) 1 NC_000962.3 Rv0879c, (MTCY31.07c), len: 91 aa. Possible conserved transmembrane protein, C-terminus highly similar to C-terminal part of MLCB57_14|O33059 conserved hypothetical protein from Mycobacterium leprae (91 aa),FASTA scores: E(): 1.2e-25, (76.9% identity in 91 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved transmembrane protein 978756 885095 Rv0879c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_215394.1 978481 R 83332 CDS NP_215395.1 15608020 885205 978934..979365 1 NC_000962.3 Rv0880, (MTCY31.08), len: 143 aa. Possible transcriptional regulator, MarR family, equivalent to MLCB57_15|O3306|NP_302411.1|NC_002677 putative MarR-family protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 818, E(): 0, (89.5% identity in 143 aa overlap). Also similar to many others e.g. CAB93410.1|AL357524 putative marR-family protein from Streptomyces coelicolor (145 aa); NP_251757.1|NC_002516 probable transcriptional regulator from Pseudomonas aeruginosa (147 aa); etc. Also similar to Rv2327 from Mycobacterium tuberculosis (163 aa).; Possible transcriptional regulatory protein (possibly MarR-family) 979365 885205 Rv0880 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (possibly MarR-family) NP_215395.1 978934 D 83332 CDS NP_215396.1 15608021 885121 979362..980228 1 NC_000962.3 Rv0881, (MTCY31.09), len: 288 aa. Possible rRNA methyltransferase, highly similar to others and hypothetical proteins e.g. CAB76071.1|AL157953 putative rRNA methylase from Streptomyces coelicolor (272 aa); NP_421117.1|NC_002696 spoU rRNA methylase family protein from Caulobacter crescentus (268 aa); D90913_93|P74261 rRNA methylase from Synechocystis sp. (274 aa), FASTA scores: E(): 1.1e-13, (26.3% identity in 278 aa overlap); P18644|TSNR_STRCN rRNA methyltransferase from Streptomyces cyaneus (Streptomyces curacoi) (269 aa), FASTA scores: E(): 3.7e-08, (23.9% identity in 268 aa overlap); etc. Equivalent to AAK45146.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 46 aa.; Possible rRNA methyltransferase (rRNA methylase) 980228 885121 Rv0881 Mycobacterium tuberculosis H37Rv Possible rRNA methyltransferase (rRNA methylase) NP_215396.1 979362 D 83332 CDS NP_215397.1 15608022 885248 980225..980509 1 NC_000962.3 Rv0882, (MTCY31.10), len: 94 aa. Probable transmembrane protein.; Probable transmembrane protein 980509 885248 Rv0882 Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_215397.1 980225 D 83332 CDS NP_215398.1 15608023 885139 complement(980506..981267) 1 NC_000962.3 Rv0883c, (MTCY31.11c), len: 253 aa. Conserved hypothetical protein, equivalent to O3306|MLCB57_16 conserved hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: E(): 0, (79.4% identity in 253 aa overlap). Also highly similar to N_terminus of AL009204|SC9B10_22 hypothetical protein from Streptomyces coelicolor (352 aa), FASTA scores: E(): 6.1e-20, (35.0% identity in 246 aa overlap).; hypothetical protein 981267 885139 Rv0883c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215398.1 980506 R 83332 CDS NP_215399.1 15608024 885140 complement(981424..982554) 1 NC_000962.3 Rv0884c, (MTCY31.12c), len: 376 aa. Possible serC,phosphoserine aminotransferase, equivalent to MLCB57_17 putative phosphoserine aminotransferase from Mycobacterium leprae (376 aa), FASTA scores: E(): 0, (87.5 identity in 376 aa overlap). Also highly similar to CAC08322.1|AL392149 putative aminotransferase from Streptomyces coelicolor (363 aa); and similar to other phosphoserine aminotransferases e.g. NP_386837.1|NC_003047 putative phosphoserine aminotransferase protein from Sinorhizobium meliloti (392 aa); P52878|SERC_METBA phosphoserine aminotransferase from Methanosarcina barkeri (370 aa); P10658|SERC_RABIT|RABEPIP_1 phosphoserine aminotransferase from Rabbit (370 aa), FASTA scores: opt: 271, E(): 3.5e-11,(24.5% identity in 368 aa overlap); etc. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate.; Possible phosphoserine aminotransferase SerC (PSAT) 982554 serC 885140 serC Mycobacterium tuberculosis H37Rv Possible phosphoserine aminotransferase SerC (PSAT) NP_215399.1 981424 R 83332 CDS NP_215400.1 15608025 885285 982762..983784 1 NC_000962.3 Rv0885, (MTCY31.13), len: 340 aa. Conserved hypothetical protein, equivalent to O33063|MLCB57_18 possible transmembrane protein from Mycobacterium leprae (341 aa), FASTA score: (83.9% identity in 341 aa overlap). Also similar except in C-terminus to T35630 probable membrane protein from Streptomyces coelicolor (312 aa).; hypothetical protein 983784 885285 Rv0885 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215400.1 982762 D 83332 CDS NP_215401.1 15608026 885195 983803..985530 1 NC_000962.3 Rv0886, (MTCY31.14), len: 575 aa. Probable fprB,ferredoxin/ferredoxin-NADP(+) reductase (NADPH:adrenodoxin oxidoreductase), equivalent to O3306|MLCB57_19 ferredoxin/ferredoxin--NADP reductase from Mycobacterium leprae (555 aa), FASTA scores: E(): 0, (76.6 identity in 560 aa overlap). Also highly similar or similar to others e.g. NP_294219.1|NC_001263 putative ferredoxin/ferredoxin--NADP reductase from Deinococcus radiodurans (479 aa) (N-terminus shorter); P22570|ADRO_HUMAN NADPH:adrenodoxin oxidoreductase from homo sapiens (497 aa), FASTA scores: opt: 624, E(): 3e-30,(39.7% identity in 484 aa overlap); P08165|ADRO_BOVIN NADPH:adrenodoxin oxidoreductase from Bos taurus (492 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv3106, Rv3858c, etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature.; Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase) 985530 fprB 885195 fprB Mycobacterium tuberculosis H37Rv Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase) NP_215401.1 983803 D 83332 CDS NP_215402.1 15608027 885113 complement(985513..985971) 1 NC_000962.3 Rv0887c, (MTCY31.15c), len: 152 aa. Conserved hypothetical protein, highly similar to others e.g. NP_436346.1|NC_003037 Hypothetical protein from Sinorhizobium meliloti (149 aa); AL132644|SCI8_26 hypothetical protein from Streptomyces coelicolor (194 aa),FASTA scores: opt: 220, E(): 1.5e-07, (33.6% identity in 131 aa overlap); etc. Also shows weak similarity with transposases and related proteins.; hypothetical protein 985971 885113 Rv0887c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215402.1 985513 R 83332 CDS NP_215403.1 15608028 885210 987233..988705 1 NC_000962.3 Rv0888, (MTCY31.16), len: 490 aa. Probable exported protein. Equivalent to AAK45157.1 from Mycobacterium tuberculosis strain CDC1551 (507 aa) but shorter 17 aa. Contains possible N-terminal signal sequence. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable exported protein 988705 885210 Rv0888 Mycobacterium tuberculosis H37Rv Probable exported protein NP_215403.1 987233 D 83332 CDS NP_215404.1 15608029 885466 complement(988740..989861) 1 NC_000962.3 Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30,(33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family.; Probable citrate synthase II CitA 989861 citA 885466 citA Mycobacterium tuberculosis H37Rv Probable citrate synthase II CitA NP_215404.1 988740 R 83332 CDS NP_215405.1 15608030 885227 complement(989948..992596) 1 NC_000962.3 Rv0890c, (MTCY31.18c), len: 882 aa. Probable transcriptional regulatory protein, LuxR family, highly similar (but shorter 238 aa in N-terminus) to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar (generally in part) to others e.g. T50568 probable multi-domain regulatory protein from Streptomyces coelicolor (1334 aa); P10957|NARL_ECOLI nitrate/nitrite response regulator protein from Escherichia coli (216 aa),FASTA scores: opt: 193, E(): 6e-06, (37.4% identity in 99 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. MTCY02B10_22, MTV008_44,MTV036_21, and MTCY31_24. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature, and probable helix-turn helix motif from aa 836 to 857 (Score 1559, +4.50 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. Alternative nucleotide at position 990001 (G->C; P866A) has been observed.; Probable transcriptional regulatory protein (probably LuxR-family) 992596 885227 Rv0890c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably LuxR-family) NP_215405.1 989948 R 83332 CDS NP_215406.1 15608031 885493 complement(992598..993455) 1 NC_000962.3 Rv0891c, (MTCY31.19c), len: 285 aa. Possible transcriptional regulator, highly similar in N-terminus to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar to several Mycobacterium tuberculosis putative transcriptional regulators e.g. Q1102|MTCY02B10_22 probable transcriptional regulatory protein (1159 aa), FASTA scores: opt: 702, E(): 8.3e-40, (50.6% identity in 247 aa overlap); MTV036_21; MTV008_44; MTCY02B10_23. Also shows similarity with several adenylate cyclases and hydrolases from other organisms.; Possible transcriptional regulatory protein 993455 885493 Rv0891c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_215406.1 992598 R 83332 CDS NP_215407.1 15608032 885225 993853..995340 1 NC_000962.3 Rv0892, (MTCY31.20), len: 495 aa. Probable monooxygenase, highly similar to others e.g. NP_250787.1|NC_002516 probable flavin-binding monooxygenase from Pseudomonas aeruginosa (491 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); P12015|CYMO_ACIS cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 489, E(): 6.8e-26, (30.3% identity in 492 aa overlap); etc. Also highly similar to Rv0565c, Rv3854c, Rv3083, etc from Mycobacterium tuberculosis. Has hydrophobic stretch at N-terminus.; Probable monooxygenase 995340 885225 Rv0892 Mycobacterium tuberculosis H37Rv Probable monooxygenase NP_215407.1 993853 D 83332 CDS NP_215408.1 15608033 885477 complement(995318..996295) 1 NC_000962.3 Rv0893c, (MTCY31.21c), len: 325 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), belongs in family with P96823|Rv0146|MTCI5.20 from Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 784, E(): 0, (43.2% identity in 308 aa overlap); Rv0726c, Rv0731c, Rv3399, etc. Also shows some similarity with others e.g. SC9B5.10|T35930 hypothetical protein from Streptomyces coelicolor (303 aa); BSUB0008_141|Q45500 hypothetical 34.8 kDa protein from Bacillus subtilis (304 aa), FASTA scores: E(): 0.00033,(26.8% identity in 168 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible S-adenosylmethionine-dependent methyltransferase 996295 885477 Rv0893c Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_215408.1 995318 R 83332 CDS NP_215409.1 15608034 885199 996524..997705 1 NC_000962.3 Rv0894, (MTCY31.22), len: 393 aa. Possible regulatory protein, LuxR family, highly similar in part to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar to others e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); NP_107293.1|NC_002678 transcriptional regulator from Mesorhizobium loti (903 aa); etc. Also similar to other regulatory proteins from Mycobacterium tuberculosis e.g. Rv2488c|MTV008_44 (1137 aa), FASTA score: (53.2% identity in 363 aa overlap); Rv1358|MTCY02B10_22 (1159 aa), FASTA score: (52.3% identity in 365 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible transcriptional regulatory protein (possibly LuxR-family) 997705 885199 Rv0894 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (possibly LuxR-family) NP_215409.1 996524 D 83332 CDS NP_215410.1 15608035 885481 997782..999299 1 NC_000962.3 Rv0895, (MTCY31.23), len: 505 aa. Possible triacylglycerol synthase (See Daniel et al., 2004); member of family with: Rv3740c, Rv3734c, Rv1425, Rv1760, etc. Shows some similarity with NP_301898.1|NC_002677 conserved membrane protein from Mycobacterium leprae (491 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 999299 885481 Rv0895 Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_215410.1 997782 D 83332 CDS NP_215411.1 15608036 885208 999472..1000767 1 NC_000962.3 Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2,citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa),FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. Also similar to two other Mycobacterium tuberculosis citrate synthases, Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (29.2% identity in 274 aa overlap) and Rv1131|MTCY22G8.20|gltA1 (393 aa). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family.; Probable citrate synthase I GltA2 1000767 gltA2 885208 gltA2 Mycobacterium tuberculosis H37Rv Probable citrate synthase I GltA2 NP_215411.1 999472 D 83332 CDS NP_215412.1 15608037 885641 complement(1000808..1002415) 1 NC_000962.3 Rv0897c, (MTCY31.25c), len: 535 aa. Possible oxidoreductase, similar to various oxidoreductases from diverse organisms e.g. CAB94055.1|AL358672 putative oxidoreductase from Streptomyces coelicolor (540 aa); NP_147877.1|NC_000854 phytoene dehydrogenase from Aeropyrum pernix (549 aa); Q01671|CRTD_RHOSH methoxyneurosporene dehydrogenase from Rhodobacter sphaeroides (495 aa), FASTA scores: opt: 139, E(): 2.6e-06, (23.8% identity in 538 aa overlap); etc. Also similar to Rv1432, Rv2997, and Rv3829c from Mycobacterium tuberculosis.; Probable oxidoreductase 1002415 885641 Rv0897c Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_215412.1 1000808 R 83332 CDS NP_215413.1 15608038 885206 complement(1002441..1002704) 1 NC_000962.3 Rv0898c, (MTCY31.26c), len: 87 aa. Conserved hypothetical protein, highly similar to CAC01589.1|AL391041 hypothetical protein from Streptomyces coelicolor (87 aa). Also shows some similarity to Rv0709|MTCY210.28|rpmC from Mycobacterium tuberculosis (77 aa), FASTA score: (28.8% identity in 73 aa overlap).; hypothetical protein 1002704 885206 Rv0898c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215413.1 1002441 R 83332 CDS NP_215414.1 15608039 885286 1002812..1003792 1 NC_000962.3 Rv0899, (MTCY31.27), len: 326 aa. OmpA, outer membrane protein A (See Senaratne et al., 1998). C-terminal region similar to C-terminus of many members of the OmpA family of outer membrane proteins, e.g. NP_458280.1|NC_003198 putative outer membrane protein from Salmonella enterica subsp. enterica serovar Typhi (220); NP_418008.1|NC_000913 putative outer membrane protein from Escherichia coli strain K12 (219 aa), FASTA scores: opt: 296, E(): 2.2e-11, (45.3% identity in 117 aa overlap); NP_231844.1|NC_002505 outer membrane protein OmpA from Vibrio cholerae (321 aa); Q05146|OMPA_BORAV outer membrane protein A precursor from Bordetella avium (194 aa); etc. A signal peptide sequence probably exists at the N-terminus. N-terminal domain is necessary and sufficient for membrane translocation (See Alahari et al., 2007). Contains PS00044 Bacterial regulatory proteins, lysR family signature. Belongs to the OmpA family. Pore-forming activity is pH-dependent.; Outer membrane protein A OmpA 1003792 ompA 885286 ompA Mycobacterium tuberculosis H37Rv Outer membrane protein A OmpA NP_215414.1 1002812 D 83332 CDS NP_215415.1 15608040 885179 1003805..1003957 1 NC_000962.3 Rv0900, (MTCY31.28), len: 50 aa. Possible membrane protein, with hydrophobic domain from aa 4-26.; Possible membrane protein 1003957 885179 Rv0900 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215415.1 1003805 D 83332 CDS NP_215416.1 15608041 885203 1003957..1004484 1 NC_000962.3 Rv0901, (MTCY31.29), len: 175 aa. Possible conserved exported or membrane protein, with hydrophobic N-terminus at aa 7-25. Shows some similarity in C-terminus to O33070|Z99494|MLCB57.59 hypothetical protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 204, E(): 3.2e-12, (44.9% identity in 78 aa overlap).; Possible conserved exported or membrane protein 1004484 885203 Rv0901 Mycobacterium tuberculosis H37Rv Possible conserved exported or membrane protein NP_215416.1 1003957 D 83332 CDS NP_215417.1 15608042 885647 complement(1004501..1005841) 1 NC_000962.3 Rv0902c, (MTCY31.30c), len: 446 aa. PrrB,two-component sensor histidine kinase (see citations below), transmembrane protein, equivalent to MLCB57_26|NP_302403.1|NC_002677 sensor histidine kinase from Mycobacterium leprae (446 aa); and similar at C-termini to NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa). C-terminus also similar to the C-termini of many sensor-like histidine kinase proteins e.g. P08336|CPXA_ECOLI|ECFB|SSD|EUP|B3911|Z5456|ECS4837 sensor protein from Escherichia coli strain K12 (457 aa), FASTA scores: opt: 364, E(): 1.7e-15, (27.1% identity in 398 aa overlap); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (483 aa); CAB82845.1|AJ277081 putative histidine kinase from Amycolatopsis mediterranei (472 aa); etc. Also similar in part to Mycobacterium tuberculosis proteins Rv3764c (475 aa); and Rv0982 (504 aa). Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999).; Two component sensor histidine kinase PrrB 1005841 prrB 885647 prrB Mycobacterium tuberculosis H37Rv Two component sensor histidine kinase PrrB NP_215417.1 1004501 R 83332 CDS NP_215418.1 15608043 885209 complement(1005852..1006562) 1 NC_000962.3 Rv0903c, (MTCY31.31c), len: 236 aa. PrrA,two-component response regulator (see citations below),equivalent to Z99494|MLCB57_27|NP_302402.1|NC_002677 two-component response regulator from Mycobacterium leprae (233 aa), FASTA scores: opt: 1414, E(): 0, (95.7% identity in 233 aa overlap); and similar to T45446 probable two-component response regulator from Mycobacterium leprae (253 aa). Also similar to many sensor-like histidine kinase proteins e.g. CAB88489.1|AL353816 putative two-component systen response regulator from Streptomyces coelicolor (248 aa); AAG36759.1|AF119221_1 |AF119221 response regulator from Corynebacterium glutamicum (232 aa); Q02540|COPR_PSESM transcriptional activator protein COPR from Pseudomonas syringae (pv. tomato) (227 aa), FASTA scores: opt: 600,E(): 0, (44.4% identity in 225 aa overlap); etc. Also similar to Rv0981 from Mycobacterium tuberculosis (230 aa),Rv3765c (234 aa), phoP (247 aa), etc. Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999).; Two component response transcriptional regulatory protein PrrA 1006562 prrA 885209 prrA Mycobacterium tuberculosis H37Rv Two component response transcriptional regulatory protein PrrA NP_215418.1 1005852 R 83332 CDS NP_215419.1 15608044 885279 complement(1006693..1008180) 1 NC_000962.3 Rv0904c, (MTCY31.32c, MT0927), len: 495 aa. Putative accD3, acetyl-CoA carboxylase carboxyl transferase, beta subunit (carboxyltransferase subunit of acetyl-CoA carboxylase), highly similar in part to AAA63045.1|U15184 zinc finger protein from Mycobacterium leprae (201 aa). Also highly similar to others e.g. CAC42827.1|Y17592 putative carboxyltransferase subunit of acetyl-CoA carboxylase from Corynebacterium glutamicum (491 aa); CAB86110.1|AL163003 putative acetyl CoA carboxylase (alpha and beta subunits) from Streptomyces coelicolor (458 aa); Q54776|ACCD_SYNP7 acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Synechococcus sp. (305 aa); P12217|ACCD_MARPO acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Marchantia polymorpha (316 aa), FASTA scores: opt: 519, E():1.6e-24, (40.2% identity in 219 aa overlap); etc. Also similar to Rv3280, Rv2502c,etc from Mycobacterium tuberculosis. Belongs to the ACCD/PCCB family.; Putative acetyl-coenzyme A carboxylase carboxyl transferase (subunit beta) AccD3 (accase beta chain) 1008180 accD3 885279 accD3 Mycobacterium tuberculosis H37Rv Putative acetyl-coenzyme A carboxylase carboxyl transferase (subunit beta) AccD3 (accase beta chain) NP_215419.1 1006693 R 83332 CDS NP_215420.1 15608045 885825 1008207..1008938 1 NC_000962.3 Rv0905, (MTCY31.33), len: 243 aa. Possible echA6,enoyl-CoA hydratase, highly similar to ML15184|U15184 enoyl-CoA hydratase from Mycobacterium leprae (247 aa),FASTA score: (85.8% identity in 247 aa overlap). Also similar to many e.g. NP_250320.1|NC_002516 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (261 aa); NP_415911.1|NC_000913 putative enzyme from Escherichia coli strain K12 (255 aa); P24162|ECHH_RHOCA|FADB1 enoyl-CoA hydratase homolog from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 404, E():7.8e-21, (37.3% identity in 249 aa overlap); etc.; Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 1008938 echA6 885825 echA6 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215420.1 1008207 D 83332 CDS NP_215421.1 15608046 885150 1008944..1010062 1 NC_000962.3 Rv0906, (MTCY31.34), len: 372 aa. Conserved protein,highly similar to others e.g. SC6A5.25|AL049485|T35416 hypothetical protein from Streptomyces coelicolor (370 aa),FASTA scores: opt: 1125, E(): 0, (51.3% identity in 335 aa overlap); NP_242955.1|NC_002570|BH2089 conserved protein from Bacillus halodurans (370 aa); etc. Also shows some similarity to C-terminus of Q48412|ROMA_KLEPN Q48412 outer membrane protein roma (fragment) from Klebsiella pneumoniae (132 aa), FASTA scores: opt: 319, E(): 8.5e-14, (46.2% identity in 104 aa overlap); NP_105215.1|NC_002678 hypothetical protein which contains similarity to outer membrane protein romA from Enterobacter cloacae (350 aa); etc. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 1010062 885150 Rv0906 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215421.1 1008944 D 83332 CDS NP_215422.1 15608047 885146 1010136..1011734 1 NC_000962.3 Rv0907, (MTCY21C12.01), len: 532 aa. Conserved protein, possibly involved in cell wall biosynthesis: similar to many beta-lactamases, penicillin-binding proteins and hypothetical proteins e.g. NP_298910.1|NC_002488 beta-lactamase from Xylella fastidiosa (455 aa); Q06317|PBP4_NOCLA penicillin-binding protein 4 (PBP-4) (381 aa), FASTA scores: opt: 299, E(): 8.8e-05, (28.7% identity in 401 aa overlap); etc. N-terminus highly similar to AAA63047.1|U15184 hypothetical protein from Mycobacterium leprae (58 aa). Related to other putative esterases and penicillin binding proteins in Mycobacterium tuberculosis e.g. Rv1730c|MTCY04C12.15c (517 aa).; hypothetical protein 1011734 885146 Rv0907 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215422.1 1010136 D 83332 CDS NP_215423.1 15608048 885657 1011731..1014124 1 NC_000962.3 Rv0908, (MTCY21C12.02), len: 797 aa. Probable ctpE,metal cation-transporting ATPase P-type, transmembrane protein, E1-E2 family, highly similar to many e.g. AB93406.1|AL357524 putative integral membrane ATPase from Streptomyces coelicolor (802 aa); NP_346063.1|NC_003028 cation-transporting ATPase (E1-E2 family) from Streptococcus pneumoniae (778 aa); P37278|ATCL_SYNP7|PACL cation-transporting atpase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (926 aa), FASTA scores: opt: 257, E(): 4.8e-33, (27.7% identity in 905 aa overlap); etc. Contains E1-E2 ATPases phosphorylation site (PS00154). Belongs to the cation transport ATPases family (E1-E2 ATPases).; Probable metal cation transporter ATPase P-type CtpE 1014124 ctpE 885657 ctpE Mycobacterium tuberculosis H37Rv Probable metal cation transporter ATPase P-type CtpE NP_215423.1 1011731 D 83332 CDS NP_215424.1 15608049 885197 1014681..1014860 1 NC_000962.3 Rv0909, (MTCY21C12.03), len: 59 aa. Conserved hypothetical protein, equivalent to NP_302399.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (56 aa). Also some similarity with AL022268|SC4H2_10c hypothetical protein from Streptomyces coelicolor (97 aa),FASTA scores: opt: 106, E(): 0.13, (43.2% identity in 37 aa overlap).; hypothetical protein 1014860 885197 Rv0909 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215424.1 1014681 D 83332 CDS NP_215425.1 15608050 885137 1014866..1015300 1 NC_000962.3 Rv0910, (MTCY21C12.04), len: 144 aa. Conserved hypothetical protein, equivalent to NP_302398.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (181 aa), FASTA scores: opt: 820, E(): 0, (83.9% identity in 143 aa overlap). Also similar to Rv1546|MTCY48.19c hypothetical protein from Mycobacterium tuberculosis (143 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1015300 885137 Rv0910 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215425.1 1014866 D 83332 CDS NP_215426.1 15608051 885093 1015398..1016171 1 NC_000962.3 Rv0911, (MTCY21C12.05), len: 257 aa. Conserved protein, showing similarity with hydroxylases and hypothetical proteins e.g. T35325 probable hydroxylase from Streptomyces coelicolor (265 aa); Q54242 hypothetical protein from Streptomyces, FASTA scores: opt: 372, E(): 8.8e-18, (32.0% identity in 256 aa overlap); AAD04716.1|U77891 doxorubicin biosynthesis enzyme DnrV from Streptomyces peucetius (275 aa); AAA63051.1|U15184 hypothetical protein from Mycobacterium leprae (94 aa); etc. Also similar to Rv0577 hypothetical protein from Mycobacterium tuberculosis (261 aa).; hypothetical protein 1016171 885093 Rv0911 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215426.1 1015398 D 83332 CDS NP_215427.1 15608052 885103 1016236..1016685 1 NC_000962.3 Rv0912, (MTCY21C12.06), len: 149 aa. Probable conserved transmembrane protein, equivalent to Q50121|NP_302397.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (144 aa), FASTA scores: opt: 677,E(): 6.9e-38, (69.5% identity in 141 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 1016685 885103 Rv0912 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215427.1 1016236 D 83332 CDS NP_215428.1 15608053 885042 complement(1017217..1018725) 1 NC_000962.3 Rv0913c, (MTCY21C12.07c), len: 502 aa. Possible dioxygenase, showing similarity with others e.g. AAK38744.1|AY029525 carotenoid 9,10-9',10' cleavage dioxygenase from Phaseolus vulgaris (543 aa); CAB56138.1|AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); AAK06796.1|AF324838_15|AF324838 putative dioxygenase SimC5 from Streptomyces antibioticus (456 aa); Q53353|S65040 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (485 aa), FASTA scores: opt: 310, E(): 3.4e-20, (28.9% identity in 495 aa overlap); etc. Also some similarity with Rv0654|MTCI376.22 probable dioxygenase from Mycobacterium tuberculosis (501 aa).; Possible dioxygenase 1018725 885042 Rv0913c Mycobacterium tuberculosis H37Rv Possible dioxygenase NP_215428.1 1017217 R 83332 CDS NP_215429.1 15608054 885046 complement(1018727..1019965) 1 NC_000962.3 Rv0914c, (MTCY21C12.08c), len: 412 aa. Possible lipid carrier protein or keto acyl-CoA thiolase, highly similar to NP_421905.1|NC_002696 thiolase family protein from Caulobacter crescentus (407 aa); and similar to others e.g. NP_107896.1|NC_002678 3-ketoacyl-CoA thiolase from Mesorhizobium loti (392 aa); NP_385796.1|NC_003047 putative 3-ketoacyl-CoA thiolase protein from Sinorhizobium meliloti (389 aa); NP_275932.1|NC_000916 lipid-transfer protein (sterol or nonspecific) from Methanothermobacter thermautotrophicus (383 aa); AB55378.1|AL117263 possible 3-ketoacyl-CoA thiolase from Leishmania major (441 aa),FASTA scores: opt: 547, E(): 3.1e-26, (31.0% identity in 435 aa overlap); etc. Also similar to Rv2790c, Rv1627c,Rv0244, etc from Mycobacterium tuberculosis. Could belong to the thiolase family.; Possible lipid carrier protein or keto acyl-CoA thiolase 1019965 885046 Rv0914c Mycobacterium tuberculosis H37Rv Possible lipid carrier protein or keto acyl-CoA thiolase NP_215429.1 1018727 R 83332 CDS YP_177765.1 57116795 885069 complement(1020058..1021329) 1 NC_000962.3 Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; PPE family protein PPE14 1021329 PPE14 885069 PPE14 Mycobacterium tuberculosis H37Rv PPE family protein PPE14 YP_177765.1 1020058 R 83332 CDS YP_177766.1 57116796 885167 complement(1021344..1021643) 1 NC_000962.3 Rv0916c, (MTCY21C12.10c), len: 99 aa. PE7 (alternate gene name: MTB10). Member of the Mycobacterium tuberculosis PE family (see citations below), similar to many e.g. Rv1788 from Mycobacterium tuberculosis (99 aa), FASTA scores: opt: 321, E(): 1.3e-11, (53.5% identity in 99 aa overlap); etc.; PE family protein PE7 1021643 PE7 885167 PE7 Mycobacterium tuberculosis H37Rv PE family protein PE7 YP_177766.1 1021344 R 83332 CDS NP_215432.1 15608057 885172 1022087..1023868 1 NC_000962.3 Rv0917, (MTCY21C12.11), len: 593 aa. Possible betP,glycine betaine transporter, integral membrane protein,highly similar to many transporters, mainly glycine betaine transporters, e.g. P54582|BETP_CORGL glycine betaine transporter from Corynebacterium glutamicum (Brevibacterium flavum) (595 aa), FASTA scores: opt: 1367, E(): 0, (42.7% identity in 504 aa overlap); T35264 probable BccT family transporter from Streptomyces coelicolor (578 aa); NP_243511.1|NC_002570 glycine betaine transporter from Bacillus halodurans (504 aa); NP_439848.1|NC_000907 high-affinity choline transport protein (betT) from Haemophilus influenzae (669 aa); etc. Seems to belong to the BCCT (TC 2.33) family of transporters.; Possible glycine betaine transport integral membrane protein BetP 1023868 betP 885172 betP Mycobacterium tuberculosis H37Rv Possible glycine betaine transport integral membrane protein BetP NP_215432.1 1022087 D 83332 CDS NP_215433.1 15608058 885198 1024211..1024687 1 NC_000962.3 Rv0918, (MTCY21C12.12), len: 158 aa. Conserved protein, similar in part to Q50116 hypothetical protein from Mycobacterium leprae (44 aa), FASTA scores: opt: 132,E(): 0.0055, (65.6% identity in 32 aa overlap). Also some similarity in C-terminus with other hypothetical proteins e.g. NP_289961.1|NC_002655 hypothetical protein from Escherichia coli strain O157:H7 (94 aa); etc.; hypothetical protein 1024687 885198 Rv0918 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215433.1 1024211 D 83332 CDS NP_215434.1 15608059 885221 1024684..1025184 1 NC_000962.3 Rv0919, (MTCY21C12.13), len: 166 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to Q50115 hypothetical protein from Mycobacterium leprae (90 aa), FASTA scores: opt: 243, E(): 5.3e-11, (56.5% identity in 85 aa overlap). Alternative nucleotide at position 1025106 (T->C; F141F) has been observed.; GCN5-related N-acetyltransferase 1025184 885221 Rv0919 Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_215434.1 1024684 D 83332 CDS NP_215435.1 15608060 885549 complement(1025497..1026816) 1 NC_000962.3 Rv0920c, (MTCY21C12.14c), len: 439 aa. Probable transposase for IS1554, highly similar to others e.g. MTCY441.35|Q45111 transposase from Mycobacterium tuberculosis (419 aa), FASTA scores: opt: 1113, E(): 0,(43.9% identity in 378 aa overlap); etc. Contains transposases mutator family signature (PS01007).; Probable transposase 1026816 885549 Rv0920c Mycobacterium tuberculosis H37Rv Probable transposase NP_215435.1 1025497 R 83332 CDS NP_215436.1 15608061 885557 1027104..1027685 1 NC_000962.3 Rv0921, (MTCY21C12.15), len: 193 aa. Possible resolvase for IS1535, highly similar to many bacterial resolvases e.g. MTCY274.17c|YX1C_MYCTU Q10831 from Mycobacterium tuberculosis (295 aa), FASTA scores: opt: 537, E(): 5.7e-29, (51.8% identity in 166 aa overlap). Presents an helix turn helix motif.; Possible resolvase 1027685 885557 Rv0921 Mycobacterium tuberculosis H37Rv Possible resolvase NP_215436.1 1027104 D 83332 CDS NP_215437.1 15608062 885564 1027685..1029337 1 NC_000962.3 Rv0922, (MTCY21C12.16), len: 550 aa. Possible transposase for IS1535, similar to many e.g. YX16_MYCTU|Q10809|MTCY274.16c from Mycobacterium tuberculosis (460 aa), FASTA scores: opt 939, E(): 0,(40.6% identity in 465 aa overlap); etc.; Possible transposase 1029337 885564 Rv0922 Mycobacterium tuberculosis H37Rv Possible transposase NP_215437.1 1027685 D 83332 CDS NP_215438.1 15608063 885568 complement(1029513..1030577) 1 NC_000962.3 Rv0923c, (MTCY21C12.17c), len: 354 aa. Conserved hypothetical protein, showing similarity with C-terminal part of AF034138|AF034138_7|yjoB hypothetical protein from Bacillus subtilis (200 aa), FASTA scores: opt: 193, E(): 4.2e-05, (32.3% identity in 167 aa overlap).; hypothetical protein 1030577 885568 Rv0923c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215438.1 1029513 R 83332 CDS YP_177767.1 57116797 885569 complement(1030578..1031864) 1 NC_000962.3 Rv0924c, (MTCY21C12.18c), len: 428 aa. MntH (alternative gene name: Nramp, Mramp), H+-dependent divalent cation-transport integral membrane protein (see citations below), equivalent to O69443|MNTH_MYCBO probable manganese transport protein MNTH (BRAMP) from Mycobacterium bovis (415 aa); and NP_302396.1|NC_002677 probable manganese transport protein from Mycobacterium leprae (426 aa). Also similar (but longer 51 aa in N-terminus) to AAA63075.1|U15184 SMF2 protein from Mycobacterium leprae (377 aa), FASTA scores: opt: 1780, E(): 0, (74.5% identity in 376 aa overlap). Also similar to many orthologues of the eukaryotic Nramp (natural resistance-associated macrophage protein), also known as mntH, e.g. NP_456951.1|NC_003198 manganese transport protein MntH from Salmonella enterica subsp. enterica serovar Typhi (413 aa); etc. Belongs to the NRAMP family.; Divalent cation-transport integral membrane protein MntH (BRAMP) (MRAMP) 1031864 mntH 885569 mntH Mycobacterium tuberculosis H37Rv Divalent cation-transport integral membrane protein MntH (BRAMP) (MRAMP) YP_177767.1 1030578 R 83332 CDS NP_215440.1 15608065 885570 complement(1031896..1032633) 1 NC_000962.3 Rv0925c, (MTCY21C12.19c), len: 245 aa. Conserved protein, similar to AL132991|SCF55_19 hypothetical protein from Streptomyces coelicolor (197 aa), FASTA scores: opt: 459, E(): 1.2e-23, (39.3% identity in 201 aa overlap).; hypothetical protein 1032633 885570 Rv0925c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215440.1 1031896 R 83332 CDS NP_215441.1 15608066 885387 complement(1032710..1033786) 1 NC_000962.3 Rv0926c, (MTCY21C12.20c), len: 358 aa. Conserved hypothetical protein, similar to Rv1059 conserved hypothetical protein from Mycobacterium tuberculosis (354 aa). Also shows some similarity to AF170923|AF170923_3 dihydrodipicolinate reductase from Mastigocladus laminosus (278 aa), FASTA scores: opt: 170, E(): 0.00088, (25.7% identity in 276 aa overlap).; hypothetical protein 1033786 885387 Rv0926c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215441.1 1032710 R 83332 CDS NP_215442.1 15608067 885571 complement(1033840..1034631) 1 NC_000962.3 Rv0927c, (MTCY21C12.21c), len: 263 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, notably 7-alpha-hydroxysteroid dehydrogenases and glucose 1-dehydrogenases e.g. P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 551, E(): 1e-26, (39.5% identity in 248 aa overlap); NP_252778.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Also similar to other dehydrogenases from Mycobacterium tuberculosis e.g. MTCY09F9.36, E():1.4e-18; MTCY369.14, E():8e-17; MTCY02B10.14, E():2.5e-14; MTCY09F9.23c, E():1.5e-13; MTCY03C7.07, E():1.9e-13. Contains PS00061 Short-chain dehydrogenases/reductases family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 1034631 885571 Rv0927c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_215442.1 1033840 R 83332 CDS YP_177768.1 57116798 885366 1034903..1036015 1 NC_000962.3 Rv0928, (MTCY21C12.22), len: 370 aa. PstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein (370 aa); and Rv0934|pstS1 phosphate-binding periplasmic lipoprotein (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters.; Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1) 1036015 pstS3 885366 pstS3 Mycobacterium tuberculosis H37Rv Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1) YP_177768.1 1034903 D 83332 CDS NP_215444.1 15608069 885585 1036028..1037002 1 NC_000962.3 Rv0929, (MTCY21C12.23), len: 324 aa. PstC2,phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_302394.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (319 aa); CAB88474.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (336 aa); NP_290359.1| NC_002655 high-affinity phosphate-specific transport system (cytoplasmic membrane component) from Escherichia coli strain O157:H7 (319 aa); etc. Also similar to Rv935|MTCY08D9.04c|PSTC1 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (338 aa). Contains binding-protein-dependent transport systems inner membrane component signature (PS00402).; Phosphate-transport integral membrane ABC transporter PstC2 1037002 pstC2 885585 pstC2 Mycobacterium tuberculosis H37Rv Phosphate-transport integral membrane ABC transporter PstC2 NP_215444.1 1036028 D 83332 CDS NP_215445.2 57116799 885589 1036999..1037925 1 NC_000962.3 Rv0930, (MTCY21C12.24), len: 308 aa. Probable pstA1,phosphate-transport integral membrane ABC transporter (see citation below), highly similar to others e.g. NP_302393.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (304 aa); CAB88473.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (354 aa) (N-terminus longer); NP_312689.1|NC_002695 phosphate transport system permease protein PstA from Escherichia coli strain O157:H7 (296 aa); etc. Also similar to Rv0936|MTCY08D9.03c|PSTA2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa).; Probable phosphate-transport integral membrane ABC transporter PstA1 1037925 pstA1 885589 pstA1 Mycobacterium tuberculosis H37Rv Probable phosphate-transport integral membrane ABC transporter PstA1 NP_215445.2 1036999 D 83332 CDS NP_215446.1 15608071 885607 complement(1037920..1039914) 1 NC_000962.3 Rv0931c, (MTCY08D9.08), len: 664 aa. PknD (alternate gene name: mbk), transmembrane serine/threonine protein kinase (see citations below), equivalent to CAB62227.1|AJ250200 putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa); and highly similar in N-terminus to P54744|PKNB_MYCLE probable serine/threonine-specific protein kinase from Mycobacterium leprae (622 aa). Also highly similar to others,particularly in N-terminal half e.g. NP_243370.1|NC_002570 serine/threonine protein kinase from Bacillus halodurans (664 aa); NP_268044.1|NC_002662 serine/threonine protein kinase from Lactococcus lactis (627 aa); etc. Also highly similar to other serine/threonine protein kinases from Mycobacterium tuberculosis e.g. pknH (626 aa), FASTA scores: opt: 1398, E: 0, (49.3% identity in 540 aa overlap); pknE (566 aa); pknB (626 aa); Rv3524 (343 aa); etc. Contains Hank's kinase subdomain. Contains two transmembrane segments, which flank a highly repetitive region, suggesting a receptor-like anchoring. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on a serine residue. Appears to be co-transcribed with Rv0932c|pstS2.; Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D) 1039914 pknD 885607 pknD Mycobacterium tuberculosis H37Rv Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D) NP_215446.1 1037920 R 83332 CDS YP_177769.1 57116800 885613 complement(1039936..1041048) 1 NC_000962.3 Rv0932c, (MTCY08D9.07), len: 370 aa. PstS2,phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa); Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 1601, E(): 0, (64.5% identity in 372 aa overlap); and Rv0934|MTCY08D9.05c|pstS1 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0928 and Rv0934c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 460, E(): 2.7e-20, (31.2% identity in 375 aa overlap). Contains prokaryotic membrane lipoprotein lipid attachment site (PS00013) at N-terminus so the leader peptide of 22 aa is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. Appears to be co-transcribed with Rv0931c|pknD|mbk.; Periplasmic phosphate-binding lipoprotein PstS2 (PBP-2) (PstS2) 1041048 pstS2 885613 pstS2 Mycobacterium tuberculosis H37Rv Periplasmic phosphate-binding lipoprotein PstS2 (PBP-2) (PstS2) YP_177769.1 1039936 R 83332 CDS NP_215448.1 15608073 885653 1041264..1042094 1 NC_000962.3 Rv0933, (MTCY08D9.06c), len: 276 aa. PstB,phosphate-transport ATP-binding protein ABC transporter (see citations below), thermostable ATPase, highly similar to others e.g. NP_348334.1|NC_003030 ATPase component of ABC-type phosphate transport system from Clostridium acetobutylicum (249 aa); NP_212352.1|NC_001318 phosphate ABC transporter ATP-binding protein (pstB) from Borrelia burgdorferi (260 aa); NP_390375.1|NC_000964 phosphate ABC transporter (ATP-binding protein) from Bacillus subtilis (269 aa), FASTA scores: opt: 762, E(): 0, (47.7% identity in 243 aa overlap); etc. Also similar to other M. tuberculosis ABC transporters e.g. MTCY253.24, E(): 2.5e-15 and MTCY359.14c, E(): 3.4e-15. Contains PS00211 ABC transporters family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). Magnesium or calcium seem to have no influence on the functionality of this enzyme.; Phosphate-transport ATP-binding protein ABC transporter PstB 1042094 pstB 885653 pstB Mycobacterium tuberculosis H37Rv Phosphate-transport ATP-binding protein ABC transporter PstB NP_215448.1 1041264 D 83332 CDS YP_177770.1 57116801 885724 1042115..1043239 1 NC_000962.3 Rv0934, (MTCY08D9.05c), len: 374 aa. PstS1 (previously known as phoS1 or phoS), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 460, E(): 5.9e-19, (31.2% identity in 375 aa overlap); and Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 435, E():1.1e-17, (30.0% identity in 380 aa overlap) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0928c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 2459, E(): 0, (100% identity in 374 aa overlap). Contains a prokaryotic membrane lipoprotein lipid attachment site (PS00013) at the N-terminus so the 23 aa leader peptide sequence is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters.; Periplasmic phosphate-binding lipoprotein PstS1 (PBP-1) (PstS1) 1043239 pstS1 885724 pstS1 Mycobacterium tuberculosis H37Rv Periplasmic phosphate-binding lipoprotein PstS1 (PBP-1) (PstS1) YP_177770.1 1042115 D 83332 CDS YP_177771.1 57116802 885644 1043299..1044315 1 NC_000962.3 Rv0935, (MTCY08D9.04c), len: 338 aa. PstC1,phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_104768.1|NC_002678|pstC phosphate ABC transporter permease protein from Mesorhizobium loti (327 aa); NP_245372.1|NC_002663|PstC PstC protein from Pasteurella multocida (320 aa); P45191|PSTC_HAEIN phosphate transport system permease from Haemophilus influenza (315 aa), FASTA scores: opt: 667, E(): 0, (36.2% identity in 309 aa overlap); etc. Also similar to Rv0929|MTCY21C12.23|PSTC2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (324 aa), FASTA scores: opt: 487, E(): 4.1e-21, (32.3% identity in 303 aa overlap); and shows slight similarity to MTCY08D9.03c|PSTA2|Rv0936 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402).; Phosphate-transport integral membrane ABC transporter PstC1 1044315 pstC1 885644 pstC1 Mycobacterium tuberculosis H37Rv Phosphate-transport integral membrane ABC transporter PstC1 YP_177771.1 1043299 D 83332 CDS NP_215451.1 15608076 885756 1044317..1045222 1 NC_000962.3 Rv0936, (MTCY08D9.03c), len: 301 aa. PstA2,phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_442269.1|NC_000911|PstA phosphate transport system permease protein from Synechocystis sp. strain PCC 6803 (287 aa); NP_232473.1|NC_002506 phosphate ABC transporter permease protein from Vibrio cholerae (289 aa); P07654|PSTA_ECOLI phosphate transport system permease from Escherichia coli (296 aa), FASTA scores: opt: 464, E(): 6.7e-24, (30.5% identity in 282 aa overlap); etc. Also similar to O86345|MTCY21C12.24|PSTA1|Rv0930 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (304 aa), FASTA scores: opt: 369, E(): 6.1e-15, (32.7% identity in 248 aa overlap). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402).; Phosphate-transport integral membrane ABC transporter PstA2 1045222 pstA2 885756 pstA2 Mycobacterium tuberculosis H37Rv Phosphate-transport integral membrane ABC transporter PstA2 NP_215451.1 1044317 D 83332 CDS NP_215452.1 15608077 885050 complement(1045199..1046020) 1 NC_000962.3 Rv0937c, (MTCY08D9.02), len: 273 aa. Mku, DNA end-binding protein, highly similar to others e.g. SC6G9.24c|T35620|AL079356 hypothetical protein from Streptomyces coelicolor (365 aa), FASTA scores: opt: 648,E(): 0, (36.5% identity in 274 aa overlap); Z99110|BSUB0007_223|NP_389224.1|NC_000964 hypothetical proteins from Bacillus subtilis (311 aa), FASTA scores: opt: 623, E(): 1.1e-31, (33.9% identity in 274 aa overlap); O28548|AE000984|AF1726|NP_070554.1|NC_000917 conserved hypothetical protein from Archaeoglobus fulgidus (286 aa),FASTA scores: opt: 583, E(): 0, (36.6% identity in 262 aa overlap); etc.; DNA end-binding protein, Mku 1046020 mku 885050 mku Mycobacterium tuberculosis H37Rv DNA end-binding protein, Mku NP_215452.1 1045199 R 83332 CDS NP_215453.1 15608078 885561 1046136..1048415 1 NC_000962.3 Rv0938, (MTCY08D9.01c, MTCY10D7.36c), len: 759 aa. ligD, ATP-dependent DNA ligase, with its C-terminus similar to N-terminal parts of many ATP-dependent DNA ligases e.g. NP_250828.1|NC_002516 probable ATP-dependent DNA ligase from Pseudomonas aeruginosa (840 aa); NP_105436.1|NC_002678 ATP-dependent DNA ligase from Mesorhizobium loti (829 aa); CAB92891.1|AL356932 probable ATP-dependent DNA ligase from Streptomyces coelicolor (326 aa); etc. The N-terminal half shows similarity with hypothetical proteins from Mycobacterium tuberculosis Rv0269c and Rv3730c; and the C-terminal half with the DNA ligases Rv3731 and Rv3062.; ATP dependent DNA ligase LigD (ATP dependent polydeoxyribonucleotide synthase) (thermostable DNA ligase) (ATP dependent polynucleotide ligase) (sealase) (DNA repair enzyme) (DNA joinase) 1048415 ligD 885561 ligD Mycobacterium tuberculosis H37Rv ATP dependent DNA ligase LigD (ATP dependent polydeoxyribonucleotide synthase) (thermostable DNA ligase) (ATP dependent polynucleotide ligase) (sealase) (DNA repair enzyme) (DNA joinase) NP_215453.1 1046136 D 83332 CDS NP_215454.1 15608079 885560 1048412..1050346 1 NC_000962.3 Rv0939, (MTCY10D7.35c), len: 644 aa. Possible bifunctional enzyme, including 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase activity, and cyclase/dehydrase activity. N-terminal part similar to many isomerases e.g. NP_343861.1|NC_002754 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Sulfolobus solfataricus (318 aa); NP_068932.1|NC_000917 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Archaeoglobus fulgidus (324 aa), FASTA scores: opt: 400,E(): 5.8e-15, (33.9% identity in 289 aa overlap); etc. And C-terminal part highly similar to many cyclases/dehydrases e.g. AAK61721.1|AY033994 cyclase-like protein from Streptomyces aureofaciens (305 aa); CAC44204.1|AL593842 cyclase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 375, E(): 2.7e-26, (35.6% identity in 284 aa overlap); NP_343860.1|NC_002754 putative Cyclase/dehydrase from Sulfolobus solfataricus (308 aa); etc. Also similar to Rv2993c hypothetical protein from Mycobacterium tuberculosis.; Possible bifunctional enzyme: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) + cyclase/dehydrase 1050346 885560 Rv0939 Mycobacterium tuberculosis H37Rv Possible bifunctional enzyme: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) + cyclase/dehydrase NP_215454.1 1048412 D 83332 CDS NP_215455.1 15608080 885412 complement(1050593..1051459) 1 NC_000962.3 Rv0940c, (MTCY10D7.34), len: 288 aa. Possible oxidoreductase, similar to hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); AAG52987.1|AF040570|Rif17 putative alkanal monooxygenase from Amycolatopsis mediterranei (356 aa); etc. Also similar to putative oxidoreductases from Mycobacterium tuberculosis such as Rv0953c|P71557|YT21_MYCTU (282 aa), FASTA scores: opt: 311,E(): 3.7e-08, (31.0% identity in 248 aa overlap), Rv3079c (275 aa), Rv0791c (347 aa), etc.; Possible oxidoreductase 1051459 885412 Rv0940c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_215455.1 1050593 R 83332 CDS NP_215456.1 15608081 885914 complement(1051544..1052317) 1 NC_000962.3 Rv0941c, (MTCY10D7.33), len: 257 aa. Conserved hypothetical protein, showing some similarity with parts of several hypothetical proteins from Streptomyces coelicolor e.g. AL035161|SC9C7_20 (860 aa), FASTA scores: opt: 197,E(): 2.6e-05, (34.2% identity in 114 aa overlap).; hypothetical protein 1052317 885914 Rv0941c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215456.1 1051544 R 83332 CDS NP_215457.1 15608082 885913 1052360..1052638 1 NC_000962.3 Rv0942, (MTCY10D7.32c), len: 92 aa. Hypothetical unknown protein.; Hypothetical protein 1052638 885913 Rv0942 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215457.1 1052360 D 83332 CDS NP_215458.1 15608083 885889 complement(1052696..1053736) 1 NC_000962.3 Rv0943c, (MTCY10D7.31), len: 346 aa. Possible monooxygenase, similar in part to others e.g. NP_250229.1|NC_002516 probable flavin-containing monooxygenase from Pseudomonas aeruginosa (527 aa); AAC36351.1|AF090329 cyclohexanone monooxygenase homolog from Pseudomonas fluorescens (437 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); etc. Also similar to putative monooxygenases from Mycobacterium tuberculosis e.g. Rv1393c|P71662|CY21B4.10C (492 aa). FASTA scores: opt: 129, E(): 8.5e-21, (27.5% identity in 236 aa overlap); Rv0892 (495 aa); Rv3049c (524 aa); etc.; Probable monooxygenase 1053736 885889 Rv0943c Mycobacterium tuberculosis H37Rv Probable monooxygenase NP_215458.1 1052696 R 83332 CDS NP_215459.1 15608084 885888 1053765..1054241 1 NC_000962.3 Rv0944, (MTCY10D7.30c), len: 158 aa. Possible formamidopyrimidine-DNA glycosylase, similar to C-terminus of formamidopyrimidine-DNA glycosylases e.g. CAB63194.1|AL133469 putative formamidopyrimidine-DNA glycosylase from Streptomyces coelicolor (287 aa); FPG_LACLA|NP_266509.1|NC_002662 formamidopyrimidine-DNA glycosylase from Lactococcus lactis subsp. lactis (273 aa),FASTA scores: opt: 246, E(): 2.4e-09, (28.9% identity in 142 aa overlap); O50606|FPG_THETH|MUTM|FPG formamidopyrimidine-DNA glycosylase from Thermus thermophilus (267 aa); etc. Also similar to C-termini of endonucleases or DNA glycosylases of Mycobacterium tuberculosis e.g. Rv3297, Rv2464c, Rv2924c. May belong to the FPG family.; Possible formamidopyrimidine-DNA glycosylase (FAPY-DNA glycosylase) 1054241 885888 Rv0944 Mycobacterium tuberculosis H37Rv Possible formamidopyrimidine-DNA glycosylase (FAPY-DNA glycosylase) NP_215459.1 1053765 D 83332 CDS NP_215460.1 15608085 885629 1054247..1055008 1 NC_000962.3 Rv0945, (MTCY10D7.29c), len: 253 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_346338.1|NC_003028 oxidoreductase (short chain dehydrogenase/reductase family) from Streptococcus pneumoniae (253 aa); AAB70845.1|AF019986|PksB from Dictyostelium discoideum (260 aa); AAF86624.1|U87786 clavaldehyde dehydrogenase from Streptomyces clavuligerus (247 aa); P37440|UCPA_ECOLI oxidoreductase from Escherichia coli (285 aa), FASTA scores: opt: 275, E(): 1.1e-12, (33.8% identity in 201 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 1055008 885629 Rv0945 Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_215460.1 1054247 D 83332 CDS NP_215461.1 15608086 885533 complement(1055024..1056685) 1 NC_000962.3 Rv0946c, (MTCY10D7.28), len: 553 aa. Probable pgi,glucose-6-phosphate isomerase, equivalent to NP_301236.1|NC_002677 glucose-6-phosphate isomerase from Mycobacterium leprae (554 aa); and P96803|G6PI_MYCSM glucose-6-phosphate isomerase from Mycobacterium smegmatis (442 aa). Also highly similar to others e.g. T36015 glucose-6-phosphate isomerase from Streptomyces coelicolor (551 aa); P11537|G6PI_ECOLI|GPI glucose-6-phosphate isomerase from Escherichia coli strains K12 and O157:H7 (549 aa), FASTA scores: opt: 1779, E(): 0, (51.4% identity in 554 aa overlap); etc. Contains PS00765 Phosphoglucose isomerase signature 1, and PS00174 Phosphoglucose isomerase signature 2. Belongs to the GPI family.; Probable glucose-6-phosphate isomerase Pgi (GPI) (phosphoglucose isomerase) (phosphohexose isomerase) (phi) 1056685 pgi 885533 pgi Mycobacterium tuberculosis H37Rv Probable glucose-6-phosphate isomerase Pgi (GPI) (phosphoglucose isomerase) (phosphohexose isomerase) (phi) NP_215461.1 1055024 R 83332 CDS NP_215463.1 15608088 885485 complement(1057646..1057963) 1 NC_000962.3 Rv0948c, (MTCY10D7.26), len: 105 aa. Chorismate mutase, AroQ class (See Prakash et al., 2005; Schneider et al., 2008), equivalent to NP_301237.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (105 aa). Also similar (except in N-terminus) to SCD63.16c|CAB82023.1|AL161755 hypothetical protein from Streptomyces coelicolor (110 aa); and to N-terminus of two chorismate mutase/prephenate dehydratase.; Chorismate mutase 1057963 885485 Rv0948c Mycobacterium tuberculosis H37Rv Chorismate mutase NP_215463.1 1057646 R 83332 CDS YP_177772.1 57116803 885442 1058260..1060575 1 NC_000962.3 Rv0949, (MTCY10D7.25c), len: 771 aa. Probable uvrD1,ATP dependent DNA helicase II (see citation below),equivalent to P_301239.1|NC_002677 putative ATP-dependent DNA helicase from Mycobacterium leprae (778 aa). Also highly similar to others e.g. CAB92660.1|AL356832 from Streptomyces coelicolor (831 aa) (N-terminus longer); P56255|PCRA_BACST from Bacillus stearothermophilus (724 aa); Q10213|YAY5_SCHPO from Schizosaccharomyces pombe (Fission yeast) (887 aa), FASTA scores: opt: 927, E(): 0,(33.5% identity in 659 aa overlap); etc. Also similar to several other UvrD-like proteins in Mycobacterium tuberculosis e.g. Rv3201c, Rv3198c, Rv3202c. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UVRD subfamily of helicases. Note that previously known as uvrD.; Probable ATP-dependent DNA helicase II UvrD1 1060575 uvrD1 885442 uvrD1 Mycobacterium tuberculosis H37Rv Probable ATP-dependent DNA helicase II UvrD1 YP_177772.1 1058260 D 83332 CDS NP_215465.1 15608090 885438 complement(1060656..1061654) 1 NC_000962.3 Rv0950c, (MTCY10D7.24), len: 332 aa. Conserved hypothetical protein, highly similar to AL035500|MLCL373.02c|T45433 hypothetical protein from Mycobacterium leprae (343 aa), FASTA scores: opt: 1500,E(): 0, (71.0% identity in 331 aa overlap). C-terminus highly similar to part of various proteins e.g. C-terminal part of NP_441943.1|NC_000911|NlpD lipoprotein from Synechocystis sp (715 aa); N-terminal part of NP_066789.1|NC_002576 putative peptidase from Rhodococcus equi (546 aa); C-terminal part of NP_212396.1|NC_001318 conserved hypothetical protein from Borrelia burgdorferi (417 aa); C-terminal part of P33648|NLPD_ECOLI|nlpd lipoprotein from Escherichia coli (379 aa), FASTA scores: opt: 276, E(): 2e-10, (29.9% identity in 234 aa overlap); etc.; hypothetical protein 1061654 885438 Rv0950c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215465.1 1060656 R 83332 CDS NP_215466.1 15608091 885434 1061964..1063127 1 NC_000962.3 Rv0951, (MTCY10D7.23c), len: 387 aa. Probable sucC,succinyl-CoA synthetase, beta chain, equivalent to AL035500|MLCL373_3|NP_301241.1|NC_002677 succinyl-CoA synthase [beta] chain from Mycobacterium leprae (393 aa),FASTA score: (86.7% identity in 391 aa overlap). Also highly similar to others e.g. AB92671.1|AL356832 succinyl-CoA synthetase beta chain from Streptomyces coelicolor (394 aa); P25126|SUCC_THEFL succinyl-CoA synthetase beta chain from Thermus aquaticus (378 aa); P07460|SUCC_ECOLI succinyl-CoA synthetase beta chain from Escherichia coli (388 aa), FASTA scores: opt: 933, E(): 0,(41.0% identity in 390 aa overlap); etc.; Probable succinyl-CoA synthetase (beta chain) SucC (SCS-beta) 1063127 sucC 885434 sucC Mycobacterium tuberculosis H37Rv Probable succinyl-CoA synthetase (beta chain) SucC (SCS-beta) NP_215466.1 1061964 D 83332 CDS NP_215467.1 15608092 885426 1063140..1064051 1 NC_000962.3 Rv0952, (MTCY10D7.22c), len: 303 aa. Probable sucD,succinyl-CoA synthetase, alpha chain, equivalent to AL035500|MLCL373_4|NP_301242.1|NC_002677 succinyl-CoA synthase [alpha] chain from Mycobacterium leprae (300 aa),FASTA score: (86.3% identity in 300 aa overlap). Also highly similar to others e.g. CAB92672.1|AL356832 from Streptomyces coelicolor (294 aa); P53591|SUCD_COXBU from Escherichia coli (288 aa), FASTA scores: opt: 855, E(): 0,(53.8% identity in 286 aa overlap); etc. Contains PS00399 ATP-citrate lyase and succinyl-CoA ligases active site, and PS00017 ATP/GTP-binding site motif A (P-loop).; Probable succinyl-CoA synthetase (alpha chain) SucD (SCS-alpha) 1064051 sucD 885426 sucD Mycobacterium tuberculosis H37Rv Probable succinyl-CoA synthetase (alpha chain) SucD (SCS-alpha) NP_215467.1 1063140 D 83332 CDS NP_215468.1 15608093 885419 complement(1064114..1064962) 1 NC_000962.3 Rv0953c, (MTCY10D7.21), len: 282 aa. Possible oxidoreductase, equivalent to CAA48222.1|X68102 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (166 aa). Similar to several hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); NP_070025.1|NC_000917 N5,N10-methylenetetrahydromethanopterin reductase (mer-2) from Archaeoglobus fulgidus (348 aa); etc. Also similar to several hypothetical proteins and oxidoreductases from Mycobacterium tuberculosis e.g. Rv2161c|O06216|Z95388|MTCY270.07 (288 aa), FASTA scores: opt: 633, E(): 0, (40.4% identity in 277 aa overlap),Rv3079c (275 aa), Rv0791c (347 aa), etc. Contains PS00201 Flavodoxin signature.; Possible oxidoreductase 1064962 885419 Rv0953c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_215468.1 1064114 R 83332 CDS NP_215469.1 15608094 885411 1065127..1066038 1 NC_000962.3 Rv0954, (MTCY10D7.20c), len: 303 aa. Probable conserved transmembrane protein, highly similar to 34KD_MYCPA|Q04959 34 kDa antigenic protein from Mycobacterium paratuberculosis (298 aa), FASTA scores: opt: 1023, E(): 7.2e-36, (59.3% identity in 305 aa overlap); AAC69251.1|U82111 34 kDa antigen precursor from Mycobacterium leprae (336 aa); and AL035500|MLCL373.06 hypothetical membrane protein from Mycobacterium leprae (297 aa), FASTA score: (55.6% identity in 315 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 1066038 885411 Rv0954 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215469.1 1065127 D 83332 CDS NP_215470.1 15608095 885408 1066078..1067445 1 NC_000962.3 Rv0955, (MTCY10D7.19c), len: 455 aa. Probable conserved integral membrane protein, highly similar to AL035500|MLCL373_6 putative membrane protein from Mycobacterium leprae (430 aa), FASTA score: (75.9% identity in 419 aa overlap); and AAL05878.1|AF411607_2|AF411607 unknown protein from Mycobacterium avium subsp. paratuberculosis (409 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved integral membrane protein 1067445 885408 Rv0955 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215470.1 1066078 D 83332 CDS NP_215471.1 15608096 885407 1067561..1068208 1 NC_000962.3 Rv0956, (MTCY10D7.18c), len: 215 aa. Probable purN,5'-phosphoribosylglycinamide formyltransferase, equivalent to AAF05726.1|AF191543_1|AF191543|PurN phosphoribosylglycinamide formyltransferase from Mycobacterium avium subsp. paratuberculosis (209 aa); and AL035500|MLCL373_7 from Mycobacterium leprae (215 aa),FASTA score: (79.4% identity in 214 aa overlap). Also highly similar to others e.g. BAA89443.1|AB003159 from Corynebacterium ammoniagenes (199 aa); NP_241498.1|NC_002570 from Bacillus halodurans (188 aa); P08179|PUR3_ECOLI|B2500 from Escherichia coli strain K12 (212 aa), FASTA scores: opt: 380, E(): 2.4e-18, (36.6% identity in 183 aa overlap); C-terminus of P16340|PUR2_DROPS trifunctional purine biosynthetic protein adenosine-3 from Drosophila pseudoobscura (Fruit fly) (1364 aa); etc.; Probable 5'-phosphoribosylglycinamide formyltransferase PurN (GART) (gar transformylase) (5'-phosphoribosylglycinamide transformylase) 1068208 purN 885407 purN Mycobacterium tuberculosis H37Rv Probable 5'-phosphoribosylglycinamide formyltransferase PurN (GART) (gar transformylase) (5'-phosphoribosylglycinamide transformylase) NP_215471.1 1067561 D 83332 CDS NP_215472.1 15608097 885406 1068205..1069776 1 NC_000962.3 Rv0957, (MTCY10D7.17c), len: 523 aa. Probable purH,bifunctional purine biosynthesis protein including 5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase and inosine-monophosphate (imp) cyclohydrolase, equivalent to AL035500|MLCL373_8 putative phosphoribosylaminoimidazolecarboxamide formyltransferase from Mycobacterium leprae (527 aa), FASTA score: (88.1% identity in 520 aa overlap); and AF05727.1|AF191543_2|AF191543|PurH from Mycobacterium avium subsp. paratuberculosis (527 aa). Also highly similar to others e.g. CAB92677.1|AL356832 bifunctional purine biosynthesis protein from Streptomyces coelicolor (523 aa); NP_388534.1|NC_000964 phosphoribosylaminoimidazole carboxy formyl formyltransferase + inosine-monophosphate cyclohydrolase from Bacillus subtilis (512 aa); P15639|PUR9_ECOLI phosphoribosylaminoimidazolecarboxamide formyltransferase from Escherichia coli (529 aa), FASTA scores: opt: 1147, E(): 0, (44.8% identity in 533 aa overlap); etc. Belongs to the PurH family.; Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase) (5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase) + inosinemonophosphate cyclohydrolase (imp cyclohydrolase) (inosinicase) (imp synthetase) (ATIC) 1069776 purH 885406 purH Mycobacterium tuberculosis H37Rv Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase) (5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase) + inosinemonophosphate cyclohydrolase (imp cyclohydrolase) (inosinicase) (imp synthetase) (ATIC) NP_215472.1 1068205 D 83332 CDS NP_215473.1 15608098 885405 1069883..1071262 1 NC_000962.3 Rv0958, (MTCY10D7.16c), len: 459 aa. Possible magnesium chelatase, similar to others (especially in N-terminal parts) e.g. NP_296313.1|NC_001263|AE002088_10 putative magnesium protoporphyrin chelatase from Deinococcus radiodurans (487 aa), FASTA scores: opt: 1148,E(): 0, (42.4% identity in 450 aa overlap); Q44498|CHLI_ANAVA magnesium-chelatase subunit CHLI from Anabaena variabilis (338 aa); T31460 probable magnesium chelatase chain I bchI from Heliobacillus mobilis (363 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible magnesium chelatase 1071262 885405 Rv0958 Mycobacterium tuberculosis H37Rv Possible magnesium chelatase NP_215473.1 1069883 D 83332 CDS NP_215474.1 15608099 885329 1071255..1073273 1 NC_000962.3 Rv0959, (MTCY10D7.15c), len: 672 aa. Conserved hypothetical protein, similar to AE002069|AE002069_12 hypothetical protein from Deinococcus radiodurans (403 aa),FASTA scores: opt: 395, E(): 1.3e-15, (26.8% identity in 426 aa overlap). Contains a single copy at the N-terminus of a short repeat found three times in the M. tuberculosis ORF O33341|MTV003.05c|AL008883.; hypothetical protein 1073273 885329 Rv0959 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215474.1 1071255 D 83332 CDS YP_007409620.1 448815747 14515852 1073327..1073548 1 NC_000962.3 Rv0959A, len: 73 aa. Possible vapB9, antitoxin, part of toxin-antitoxin (TA) operon with Rv0960 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv1721c; Possible antitoxin VapB9 1073548 vapB9 14515852 vapB9 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB9 YP_007409620.1 1073327 D 83332 CDS NP_215475.1 15608100 885158 1073545..1073928 1 NC_000962.3 Rv0960, (MTCY10D7.14c), len: 127 aa. Possible vapC9,toxin, part of toxin-antitoxin (TA) operon with Rv0959A,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0065|MTV030.08 (133 aa), FASTA scores: E(): 1.5e-14, (38.3% identity in 128 aa overlap), Rv1720c (129 aa), and Rv0549c (137 aa).; Possible toxin VapC9 1073928 vapC9 885158 vapC9 Mycobacterium tuberculosis H37Rv Possible toxin VapC9 NP_215475.1 1073545 D 83332 CDS NP_215476.1 15608101 885173 1074074..1074421 1 NC_000962.3 Rv0961, (MTCY10D7.13c), len: 115 aa. Probable integral membrane protein.; Probable integral membrane protein 1074421 885173 Rv0961 Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_215476.1 1074074 D 83332 CDS NP_215477.1 15608102 885177 complement(1074440..1075114) 1 NC_000962.3 Rv0962c, (MTCY10D7.12), len: 224 aa. Possible lprP,lipoprotein. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Possible lipoprotein LprP 1075114 lprP 885177 lprP Mycobacterium tuberculosis H37Rv Possible lipoprotein LprP NP_215477.1 1074440 R 83332 CDS NP_215478.1 15608103 885184 complement(1075297..1076097) 1 NC_000962.3 Rv0963c, (MTCCY10D7.11), len: 266 aa. Conserved hypothetical protein, similar in part to other conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv2797c|MTCY16B7.46 (562 aa), FASTA scores: E(): 1.2e-23,(39.0% identity in 254 aa overlap); Rv2542 (403 aa); Rv2079 (656 aa). Also similar in part to AL133423|SC4A7_3 hypothetical secreted protein from Streptomyces coelicolor (406 aa), FASTA scores: opt: 231, E(): 6.8e-07, (31.4% identity in 204 aa overlap); and SCH10.21c|T36533 hypothetical protein from Streptomyces coelicolor (329 aa).; hypothetical protein 1076097 885184 Rv0963c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215478.1 1075297 R 83332 CDS NP_215479.1 15608104 885186 complement(1076196..1076678) 1 NC_000962.3 Rv0964c, (MTCY10D7.10), len: 160 aa. Hypothetical unknown protein. Equivalent to AAK45241.1 from Mycobacterium tuberculosis strain CDC1551 (138 aa) but longer 22 aa.; Hypothetical protein 1076678 885186 Rv0964c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215479.1 1076196 R 83332 CDS NP_215480.1 15608105 885230 complement(1076778..1077197) 1 NC_000962.3 Rv0965c, (MTCY10D7.09), len: 139 aa. Conserved hypothetical protein, showing weak similarity with Rv2798c|MTCY16B7.45 conserved hypothetical protein from Mycobacterium tuberculosis (108 aa), FASTA scores: E(): 5.6e-12, (38.9% identity in 90 aa overlap). Equivalent to AAK45242.1 from Mycobacterium tuberculosis strain CDC1551 (146 aa) but shorter 7 aa.; hypothetical protein 1077197 885230 Rv0965c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215480.1 1076778 R 83332 CDS NP_215481.2 57116804 885043 complement(1077233..1077835) 1 NC_000962.3 Rv0966c, (MTCY10D7.08), len: 200 aa. Conserved protein, equivalent to AL035500|MLCL373_12 conserved hypothetical protein from Mycobacterium leprae (200 aa),FASTA scores: opt: 1080, E(): 0, (79.5% identity in 200 aa overlap). Also highly similar to SCE6.30c|CAB88834.1|AL353832 hypothetical protein from Streptomyces coelicolor (277 aa). Some similarity to Rv2862c|MTV007.08 conserved hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: E(): 3.1e-06, (31.5% identity in 184 aa overlap). Equivalent to AAK45243.1 from Mycobacterium tuberculosis strain CDC1551 (230 aa) but shorter 30 aa. Note that Rv0966c has been shortened since first entry.; hypothetical protein 1077835 885043 Rv0966c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215481.2 1077233 R 83332 CDS NP_215482.1 15608107 885312 1077975..1078334 1 NC_000962.3 Rv0967, (MTCY10D7.07c), len: 119 aa. CsoR,copper-sensitive operon repressor, part of cso operon (See Liu et al., 2007), similar to hypothetical proteins from several organisms e.g. AE002074|AE002074_11 from Deinococcus radiodurans (102 aa), FASTA scores: opt: 233,E(): 8.6e-10, (47.0% identity in 83 aa overlap); O32222|Z99121|YVGZ from Bacillus subtilis (101 aa), FASTA scores: opt:228, E(): 3.2e-15, (38.0% identity in 92 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0190, and Rv1766.; Copper-sensitive operon repressor CsoR 1078334 csoR 885312 csoR Mycobacterium tuberculosis H37Rv Copper-sensitive operon repressor CsoR NP_215482.1 1077975 D 83332 CDS NP_215483.1 15608108 885052 1078391..1078687 1 NC_000962.3 Rv0968, (MTCY10D7.06c), len: 98 aa. Conserved protein, part of cso operon, similar to NP_301579.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (92 aa). Also highly similar to conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv3269 (93 aa), FASTA score: (51.1% identity in 94 aa overlap); and Rv1993c (90 aa).; hypothetical protein 1078687 885052 Rv0968 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215483.1 1078391 D 83332 CDS NP_215484.3 448824751 885254 1078743..1081055 1 NC_000962.3 Rv0969, (MTCY10D7.05c), len: 770 aa. Probable ctpV,metal cation transporter P-type ATPase (transmembrane protein) (see citation below), part of cso operon, highly similar (except in N-terminus) to others e.g. NP_391230.1|NC_000964 similar to heavy metal-transporting ATPase from Bacillus subtilis (803 aa); P37279|ATCS_SYNP7|PACS cation-transporting ATPase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (747 aa), FASTA scores: opt: 1851, E(): 0, (52.1% identity in 664 aa overlap); etc. Equivalent to AAK45246.1 from Mycobacterium tuberculosis strain CDC1551 (792 aa) but shorter 22 aa. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases).; Probable metal cation transporter P-type ATPase CtpV 1081055 ctpV 885254 ctpV Mycobacterium tuberculosis H37Rv Probable metal cation transporter P-type ATPase CtpV NP_215484.3 1078743 D 83332 CDS NP_215485.1 15608110 885242 1081052..1081684 1 NC_000962.3 Rv0970, (MTCY10D7.04c), len: 210 aa. Probable conserved integral membrane protein, part of cso operon,equivalent to NP_302348.1|NC_002677 probable integral membrane protein from Mycobacterium leprae (210 aa).; Probable conserved integral membrane protein 1081684 885242 Rv0970 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215485.1 1081052 D 83332 CDS NP_215486.1 15608111 885308 complement(1081775..1082584) 1 NC_000962.3 Rv0971c, (MTCY10D7.03), len: 269 aa. Probable echA7,enoyl-CoA hydratase, similar to many e.g. CAB95895.1|AL359988 putative enoyl CoA hydratase from Streptomyces coelicolor (247 aa); P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (257 aa),FASTA scores: opt: 369, E(): 2.6e-15, (33.7% identity in 246 aa overlap); etc.; Probable enoyl-CoA hydratase EchA7 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 1082584 echA7 885308 echA7 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA7 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215486.1 1081775 R 83332 CDS NP_215487.1 15608112 885237 complement(1082584..1083750) 1 NC_000962.3 Rv0972c, (MTCY10D7.02), len: 388 aa. fadE12,acyl-CoA dehydrogenase, highly similar to many e.g. CAB95893.1|AL359988 putative acyl CoA dehydrogenase from Streptomyces coelicolor (382 aa); P45857|ACDB_BACSU from Bacillus subtilis (379 aa), FASTA scores: opt: 576, E(): 2.3e-26, (29.7% identity in 381 aa overlap); etc.; Acyl-CoA dehydrogenase FadE12 1083750 fadE12 885237 fadE12 Mycobacterium tuberculosis H37Rv Acyl-CoA dehydrogenase FadE12 NP_215487.1 1082584 R 83332 CDS NP_215488.1 15608113 885922 complement(1083747..1085750) 1 NC_000962.3 Rv0973c, (MTV044.01c, MTCY10D7.01), len: 667 aa. Probable accA2 (alternate gene name: bccA),acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], highly similar to others e.g. CAB95892.1|AL359988 putative acetyl/propionyl CoA carboxylase alpha subunit from Streptomyces coelicolor (614 aa); NP_250702.1|NC_002516 probable acyl-CoA carboxylase alpha chain from Pseudomonas aeruginosa (655 aa); NP_420971.1|NC_002696 acetyl/propionyl-CoA carboxylase alpha subunit from Caulobacter crescentus ( 654 aa); NP_251581.1|NC_002516 probable biotin carboxylase/biotin carboxyl carrier protein from Pseudomonas aeruginosa (661 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv2501c|P46401|MTCY07A7.07c|BCCA_MYCTU|ACCA1 probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) (654 aa), FASTA scores, opt: 250, E(): 4e-09,(28.6% identity in 182 aa overlap); and Rv3285|MTCY71.25|ACCA3 (600 aa); Z83018|MTCY349_20 (1127 aa), FASTA scores: opt: 838, E(): 0, (40.2% identity in 500 aa overlap). Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site.; Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA2: biotin carboxylase + biotin carboxyl carrier protein (BCCP) 1085750 accA2 885922 accA2 Mycobacterium tuberculosis H37Rv Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA2: biotin carboxylase + biotin carboxyl carrier protein (BCCP) NP_215488.1 1083747 R 83332 CDS NP_215489.1 15608114 886064 complement(1085756..1087345) 1 NC_000962.3 Rv0974c, (MTV044.02c), len: 529 aa. Probable accD2,acetyl-/propionyl-CoA carboxylase (beta subunit), highly similar to many e.g. CAB95891.1|AL35998 putative acetyl/propionyl CoA carboxylase beta subunit from Streptomyces coelicolor (532 aa); NP_250704.1|NC_002516 probable acyl-CoA carboxyltransferase beta chain from Pseudomonas aeruginosa (535 aa); BAB16296.1|AB039884 acetyl-CoA carboxylase carboxyltransferase from Myxococcus xanthus (538 aa); NP_420973.1|NC_002696 putative propionyl-CoA carboxylase beta subunit from Caulobacter crescentus (530 aa); etc. Also similar to other from Mycobacterium tuberculosis: Rv2502c|ACCD1, Rv3799c|ACCD4,etc. Could belong to the ACCD/PCCB family.; Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD2 1087345 accD2 886064 accD2 Mycobacterium tuberculosis H37Rv Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD2 NP_215489.1 1085756 R 83332 CDS NP_215490.1 15608115 885856 complement(1087348..1088496) 1 NC_000962.3 Rv0975c, (MTV044.03c), len: 382 aa. Probable fadE13,acyl-CoA dehydrogenase, highly similar to many e.g. T35427 probable acyl-CoA dehydrogenase from Streptomyces coelicolor (382 aa); M74096|HUMACADL_1 Human long chain acyl-CoA dehydrogenase from Homo sapiens (430 aa), FASTA scores: opt: 819, E(): 0, (37.0% identity in 376 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. fadE20|Z98209|MTCY154_4 (386 aa), FASTA scores: (40.3% identity in 375 aa overlap). Contains PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE13 1088496 fadE13 885856 fadE13 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE13 NP_215490.1 1087348 R 83332 CDS NP_215491.1 15608116 885860 complement(1088493..1090175) 1 NC_000962.3 Rv0976c, (MTV044.04c), len: 560 aa. Conserved hypothetical protein, highly similar to others e.g. CAB95890.1|AL359988 conserved hypothetical protein from Streptomyces coelicolor (558 aa); P_251576.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (600 aa); etc. N-terminal part highly similar to AL035500|MLCL373_14 probable pseudogene from Mycobacterium leprae (163 aa),FASTA score: (50.0% identity in 122 aa overlap).; hypothetical protein 1090175 885860 Rv0976c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215491.1 1088493 R 83332 CDS YP_177773.1 57116805 885264 1090373..1093144 1 NC_000962.3 Rv0977, (MTV044.05), len: 923 aa. PE_PGRS16, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to other PGRS-type sequences e.g. AL0091|MTV004_1 from Mycobacterium tuberculosis (1125 aa), FASTA score: (45.4% identity in 959 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (51.5% identity in 750 aa overlap); etc.; PE-PGRS family protein PE_PGRS16 1093144 PE_PGRS16 885264 PE_PGRS16 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS16 YP_177773.1 1090373 D 83332 CDS YP_177774.1 57116806 885077 complement(1093361..1094356) 1 NC_000962.3 Rv0978c, (MTV044.06c), len: 331 aa. PE_PGRS17,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (73.6% identity in 277 aa overlap); etc.; PE-PGRS family protein PE_PGRS17 1094356 PE_PGRS17 885077 PE_PGRS17 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS17 YP_177774.1 1093361 R 83332 CDS NP_215494.2 57116807 885063 complement(1094670..1094864) 1 NC_000962.3 Rv0979c, (MTV044.07c), len: 64 aa (unlikely ORF). Hypothetical unknown protein. Start codon changed since first submission (-44 aa).; Hypothetical protein 1094864 885063 Rv0979c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215494.2 1094670 R 83332 CDS YP_177635.1 57116808 3205057 1094886..1095059 1 NC_000962.3 Rv0979A, len: 57 aa. rpmF, 50S ribosomal protein L32, similar to others e.g. rpmF|Q9RL50 probable 50S ribosomal protein from Streptomyces coelicolor (56 aa),FASTA scores: E(): 5.1e-09, (63.45% identity in 52 aa overlap); etc. Belongs to the L32P family of ribosomal proteins.; 50S ribosomal protein L32 RpmF 1095059 rpmF 3205057 rpmF Mycobacterium tuberculosis H37Rv 50S ribosomal protein L32 RpmF YP_177635.1 1094886 D 83332 CDS YP_177775.1 57116809 885327 complement(1095078..1096451) 1 NC_000962.3 Rv0980c, (MTV044.08c), len: 457 aa. PE_PGRS18,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002),highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (66.7% identity in 405 aa overlap); Z95844|MTCY493_2 from Mycobacterium tuberculosis (741 aa), FASTA score: (53.0% identity in 394 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008).; PE-PGRS family protein PE_PGRS18 1096451 PE_PGRS18 885327 PE_PGRS18 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS18 YP_177775.1 1095078 R 83332 CDS NP_215496.2 448824752 885038 1096822..1097508 1 NC_000962.3 Rv0981, (MTV044.09), len: 228 aa. MprA,mycobacterial persistence regulator, a two-component response regulator whose expression is required for entrance into and maintenance of persistent infection (see citation below), equivalent to NP_301250.1|NC_002677 putative two-component response regulator from Mycobacterium leprae (228 aa); and highly similar to others from Mycobacterium leprae. Also highly similar to others e.g. AAG36759.1|AF119221_1|AF119221 response regulator from Corynebacterium glutamicum (232 aa); CAB88489.1|AL353816 putative two-component system response regulator from Streptomyces coelicolor (248 aa); BJY09666_1 two-component response regulator (ragA, ragB and rpoH3) from B.japonicum (226 aa), FASTA score: (43.8% identity in 224 aa overlap); BSAJ2571_44 two-component response regulator from Bacillus subtilis (228 aa), FASTA score: (46.4% identity in 224 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv1033c (257 aa); Rv0903c (236 aa), FASTA score: (50.7 identity in 225 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. Start changed since first submission (-2 aa). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006).; Mycobacterial persistence regulator MRPA (two component response transcriptional regulatory protein) 1097508 mprA 885038 mprA Mycobacterium tuberculosis H37Rv Mycobacterial persistence regulator MRPA (two component response transcriptional regulatory protein) NP_215496.2 1096822 D 83332 CDS NP_215497.1 15608122 885062 1097508..1099022 1 NC_000962.3 Rv0982, (MTV044.10), len: 504 aa. MprB, two component sensor kinase, probable transmembrane protein (see citation below), equivalent to AL035500|MLCL373_16|NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa), FASTA score: (81.0% identity in 521 aa overlap). Also highly similar to others (especially in C-terminal part) e.g. AAG36760.1|AF119221_2|AF119221 sensor kinase from Corynebacterium glutamicum (455 aa); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (481 aa); X58793|SLCUTRS_2 sensor kinase from S.lividans (414 aa), FASTA scores: opt: 451, E(): 4.2e-21, (36.0% identity in 303 aa overlap); P30847|BAES_ECOLI sensor protein from Escherichia coli (467 aa), FASTA scores: opt: 412, E(): 1.3e-18, (30.4% identity in 336 aa overlap); etc. Also similar in C-terminal region to C-terminus of Rv0902c|Z73101|MTCY31_33 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 423, E(): 2.6e-19, (28.4 identity in 462 aa overlap). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006).; Two component sensor kinase MprB 1099022 mprB 885062 mprB Mycobacterium tuberculosis H37Rv Two component sensor kinase MprB NP_215497.1 1097508 D 83332 CDS NP_215498.1 15608123 885382 1099066..1100460 1 NC_000962.3 Rv0983, (MTV044.11), len: 464 aa. Probable pepD (alternate gene name: mtb32b), secreted or membrane serine protease (see citation below), equivalent (but longer 18 aa in N-terminus) to AL035500|MLCL373_17|T45448 probable serine proteinase from Mycobacterium leprae (452 aa), FASTA score: (74.2% identity in 466 aa overlap); and highly similar to others from Mycobacterium leprae. Also highly similar (except in N-terminus) to other proteases e.g. CAC01350.1|AL390975 putative protease from Streptomyces coelicolor (542 aa); NP_440705.1|NC_000911|HtrA serine protease from Synechocystis sp. (452 aa); NP_346646.1|NC_003028 serine protease from Streptococcus pneumoniae (393 aa); etc. Also similar in part to members of the htrA-antigen family e.g. U87242|MTU87242_3|HtrA serine protease from M. tuberculosis (542 aa), FASTA scores: opt: 846, E(): 2e-28, (40.6% identity in 392 aa overlap); and similar to other hypothetical serine proteases e.g. Rv0983, Rv0125, etc. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae,M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable serine protease PepD (serine proteinase) (MTB32B) 1100460 pepD 885382 pepD Mycobacterium tuberculosis H37Rv Probable serine protease PepD (serine proteinase) (MTB32B) NP_215498.1 1099066 D 83332 CDS NP_215499.1 15608124 885378 1100460..1101005 1 NC_000962.3 Rv0984, (MTV044.12), len: 181 aa. Possible moaB2,pterin-4-alpha-carbinolamine dehydratase, highly similar to NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa),FASTA score: (92.3% identity in 181 aa overlap). Also similar to others e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); Q56208|MOCB_SYNP7 molybdenum cofactor biosynthesis protein CB from Synechococcus sp. (319 aa),FASTA score: (37.3% identity in 142 aa overlap); C-terminus of NP_197599.1|NC_003076 molybdopterin biosynthesis CNX1 protein from Arabidopsis thaliana (670 aa); etc. Also similar to Rv0865|MOG from Mycobacterium tuberculosis (160 aa); and other mog proteins e.g. CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa). Could belong to the pterin-4-alpha-carbinolamine dehydratase family. Alternative start codon has been suggested in position 1100508.; Possible pterin-4-alpha-carbinolamine dehydratase MoaB2 (PHS) (4-alpha-hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) 1101005 moaB2 885378 moaB2 Mycobacterium tuberculosis H37Rv Possible pterin-4-alpha-carbinolamine dehydratase MoaB2 (PHS) (4-alpha-hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) NP_215499.1 1100460 D 83332 CDS NP_215500.1 15608125 885368 complement(1101025..1101480) 1 NC_000962.3 Rv0985c, (MTV044.13c), len: 151 aa. Possible mscL,large conductance mechanosensitive ion channel (integral membrane protein) (see citations below, equivalent to AL035500|MLCL373_19|NP_301254.1|NC_002677 putative mechanosensitive channel protein from Mycobacterium leprae (154 aa), FASTA score: (71.0% identity in 155 aa overlap). Also highly similar to others e.g. NP_268999.1|NC_002737 putative large conductance mechanosensitive channel from Streptococcus pyogenes (120 aa); CAB90974.1|AL355832 putative mechanosensitive channel from Streptomyces coelicolor (156 aa); Q9X722|MSCL_CLOHI large-conductance mechanosensitive channel from Clostridium histolyticum (133 aa); Z83337|BSZ83337_6 large conductance mechanosensitive channel from Bacillus subtilis (130 aa), FASTA scores: opt: 248, E(): 8.4e-10, (39.0% identity in 136 aa overlap); U08371|ECU08371_1 large conductance mechanosensitive channel from Escherichia coli strain K-12 (136 aa), FASTA score: (36.6% identity in 134 aa overlap); etc. Belongs to the MscL family.; Possible large-conductance ion mechanosensitive channel MscL 1101480 mscL 885368 mscL Mycobacterium tuberculosis H37Rv Possible large-conductance ion mechanosensitive channel MscL NP_215500.1 1101025 R 83332 CDS NP_215501.1 15608126 885364 1101803..1102549 1 NC_000962.3 Rv0986, (MTV044.14), len: 248 aa. Probable ATP-binding protein ABC transporter supposedly involved in transport of adhesion component (see citation below),highly similar to many ATP-binding proteins e.g. AE0010|AE001033_8 ABC transporter ATP-binding protein from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 669,E(): 0, (45.7% identity in 219 aa overlap); CAB81857.1|AL161691 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa); X84019|ZMDNAGRP_4 glutamate uptake regulatory protein (grp) from Z.mobilis (232 aa), FASTA score: (44.4% identity in 225 aa overlap); Z99111|BSUB0008_108 from Bacillus subtilis (230 aa), FASTA score: (38.7% identity in 222 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007).; Probable adhesion component transport ATP-binding protein ABC transporter 1102549 885364 Rv0986 Mycobacterium tuberculosis H37Rv Probable adhesion component transport ATP-binding protein ABC transporter NP_215501.1 1101803 D 83332 CDS NP_215502.1 15608127 885363 1102542..1105109 1 NC_000962.3 Rv0987, (MTV044.15, MTCI237.01), len: 855 aa. Probable transmembrane protein ABC transporter supposedly involved in transport of adhesion component (see citation below), whose N-terminus shows similarity with hypothetical proteins, generally transmembrane proteins, e.g. CAB96016.1|AL360055 putative ABC transport system integral membrane protein from Streptomyces coelicolor (855 aa); P44252|YCFU_HAEIN|HI1555 hypothetical protein from Haemophilus influenzae (393 aa), FASTA scores: opt: 265,E(): 1.7e-09, (23.6% identity in 402 aa overlap); etc. N-and C-termini respectively show similarity to O32735 ATTF protein (420 aa), FASTA scores: E(): 1e-09, (26.7% identity in 430 aa overlap), and G2340078 ATTG protein (359 aa),FASTA scores: E(): 2.7e-08, (27.8% identity in 356 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al.,2007).; Probable adhesion component transport transmembrane protein ABC transporter 1105109 885363 Rv0987 Mycobacterium tuberculosis H37Rv Probable adhesion component transport transmembrane protein ABC transporter NP_215502.1 1102542 D 83332 CDS NP_215503.1 15608128 885353 1105116..1106276 1 NC_000962.3 Rv0988, (MTCI237.02), len: 386 aa. Possible conserved exported protein, with potential N-terminal signal sequence, similar (except in N-terminus) to O32737|L63540 ATTH protein from Agrobacterium tumefaciens (355 aa), FASTA scores: opt: 651, E(): 5.7e-33, (33.4% identity in 344 aa overlap); and NP_231265.1|NC_002505 conserved hypothetical protein from Vibrio cholerae (372 aa). Predicted to be an outer membrane protein (See Song et al., 2008). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007).; Possible conserved exported protein 1106276 885353 Rv0988 Mycobacterium tuberculosis H37Rv Possible conserved exported protein NP_215503.1 1105116 D 83332 CDS NP_215504.1 15608129 885355 complement(1106405..1107382) 1 NC_000962.3 Rv0989c, (MTCI237.03c), len: 325 aa. Probable grcC2,polyprenyl diphosphate synthetase, highly similar to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl e.g. NP_471378.1|NC_003212 protein similar to heptaprenyl diphosphate synthase component II (menaquinone biosynthesis) from Listeria innocua (321 aa); NP_371994.1|NC_002758 heptaprenyl diphosphate syntase component II from Staphylococcus aureus subsp. aureus Mu50 (319 aa); P55785|HEP2_BACST heptaprenyl diphosphate synthase component from Bacillus subtilis (323 aa), FASTA scores: opt: 496, E(): 1.4e-24, (31.4% identity in 306 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins e.g. Rv0562|grcC1|NP_215076.1|MTCY25D10.41 probable polyprenyl-diphosphate synthase (335 aa); Rv3383, Rv3398c,Rv2173, etc. Seems to belong to the FPP/GGPP synthetases family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable polyprenyl-diphosphate synthase GrcC2 (polyprenyl pyrophosphate synthetase) 1107382 grcC2 885355 grcC2 Mycobacterium tuberculosis H37Rv Probable polyprenyl-diphosphate synthase GrcC2 (polyprenyl pyrophosphate synthetase) NP_215504.1 1106405 R 83332 CDS NP_215505.1 15608130 885343 complement(1107443..1108099) 1 NC_000962.3 Rv0990c, (MTCI237.04c), len: 218 aa. Hypothetical unknown protein.; Hypothetical protein 1108099 885343 Rv0990c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215505.1 1107443 R 83332 CDS NP_215506.1 15608131 885350 complement(1108172..1108504) 1 NC_000962.3 Rv0991c, (MTCI237.05c), len: 110 aa. Conserved ser-rich protein (especially in C-terminus), highly similar to N-terminus of NP_301255.1|NC_002677 conserved hypothetical protein (Ser-rich C-terminus) from Mycobacterium leprae (99 aa). Also highly similar to SCE22.04|AB90971.1|AL355832 hypothetical protein from Streptomyces coelicolor (110 aa); and similar to others.; Conserved serine rich protein 1108504 885350 Rv0991c Mycobacterium tuberculosis H37Rv Conserved serine rich protein NP_215506.1 1108172 R 83332 CDS NP_215507.1 15608132 885337 complement(1108578..1109171) 1 NC_000962.3 Rv0992c, (MTCI237.06c), len: 197 aa. Conserved hypothetical protein, equivalent to NP_301256.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (197 aa). Also similar, except in N-terminus, to other hypothetical proteins and ligases e.g. SCE87.34|CAB59679.1|AL132674 hypothetical protein from Streptomyces coelicolor (204 aa); NP_461977.1|NC_003197 putative ligase from Salmonella typhimurium (182 aa); P09160|YGFA_ECOLI hypothetical 21.1 kDa protein from Escherichia coli (182 aa), FASTA scores: opt: 191, E(): 1.1e-09, (29.5% identity in 146 aa overlap); etc.; hypothetical protein 1109171 885337 Rv0992c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215507.1 1108578 R 83332 CDS NP_215508.1 15608133 885341 1109272..1110192 1 NC_000962.3 Rv0993, (MTCI237.07), len: 306 aa. GalU,UTP--glucose-1-phosphate uridylyltransferase, equivalent to AL035500|MLCL373_22 putative UTP-glucose-1-phosphate uridylyltransferase from Mycobacterium leprae (306 aa),FASTA score: (89.7% identity in 302 aa overlap). Also highly similar to others e.g. AB59678.1|AL132674 UTP-glucose-1-phosphate uridylyltransferase from Streptomyces coelicolor (303 aa); NP_244519.1|NC_002570 UTP-glucose-1-phosphate uridylyltransferase from Bacillus halodurans (297 aa); P25520|GALU_ECOLI|B1236|Z2012|ECS17 UTP--glucose-1-phosphate uridylyltransferase from Escherichia coli strains K12 and O157:H7 (301 aa), FASTA scores: opt: 624, E(): 2.4e-33, (38.8% identity in 299 aa overlap); etc. Belongs to the prokaryotic UDPGP family.; UTP--glucose-1-phosphate uridylyltransferase GalU (UDP-glucose pyrophosphorylase) (UDPGP) (alpha-D-glucosyl-1-phosphate uridylyltransferase) (uridine diphosphoglucose pyrophosphorylase) 1110192 galU 885341 galU Mycobacterium tuberculosis H37Rv UTP--glucose-1-phosphate uridylyltransferase GalU (UDP-glucose pyrophosphorylase) (UDPGP) (alpha-D-glucosyl-1-phosphate uridylyltransferase) (uridine diphosphoglucose pyrophosphorylase) NP_215508.1 1109272 D 83332 CDS YP_177776.1 57116810 885404 1110269..1111549 1 NC_000962.3 Rv0994, (MTCI237.08), len: 426 aa. Probable moeA1,molybdenum cofactor biosynthesis protein, equivalent to AL035500|MLCL373_23 putative molybdopterin biosynthesis protein from Mycobacterium leprae (424 aa), FASTA score: (88.3% identity in 426 aa overlap). Also highly similar to many e.g. CAB59677.1|AL132674 molybdopterin biosynthesis protein from Streptomyces coelicolor (424 aa); NP_385769.1|NC_003047 probable molybdopterin biosynthesis protein from Sinorhizobium meliloti (406 aa); P12281|MOEA_ECOLI molybdopterin biosynthesis moea protein from Escherichia coli (411 aa), FASTA scores: opt: 519,E(): 1.3e-24, (32.3% identity in 402 aa overlap); etc. Also similar to MOEA2|Rv0438c|MTV037.02c probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (405 aa). Note that previously known as moeA.; Probable molybdopterin biosynthesis protein MoeA1 1111549 moeA1 885404 moeA1 Mycobacterium tuberculosis H37Rv Probable molybdopterin biosynthesis protein MoeA1 YP_177776.1 1110269 D 83332 CDS NP_215510.1 15608135 885399 1111612..1112223 1 NC_000962.3 Rv0995, (MTCI237.09), len: 203 aa. RimJ,ribosomal-protein-alanine acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to AL035500|MLCL373_24 probable acyltransferase from Mycobacterium leprae (218 aa), FASTA scores: (86.0% identity in 200 aa overlap). Also similar to others and many acyltransferases e.g. BAB69252.1|AB070946 possible acyltransferase from Streptomyces avermitilis (156 aa); NP_385025.1|NC_003047 probable ribosomal-protein-alanine acetyltransferase from Sinorhizobium meliloti (203 aa); P09454|RIMJ_ECOLI|B1066|Z1703|ECS1444 ribosomal-protein-alanine acetyltransferase from Escherichia coli strains K12 and O157:H7 (194 aa), FASTA scores: opt: 247, E(): 1.5e-10, (26.9% identity in 186 aa overlap). Belongs to the acetyltransferase family, RIMJ subfamily.; Ribosomal-protein-alanine acetyltransferase RimJ (acetylating enzyme for N-terminal of ribosomal protein S5) 1112223 rimJ 885399 rimJ Mycobacterium tuberculosis H37Rv Ribosomal-protein-alanine acetyltransferase RimJ (acetylating enzyme for N-terminal of ribosomal protein S5) NP_215510.1 1111612 D 83332 CDS NP_215511.1 15608136 885393 1112384..1113460 1 NC_000962.3 Rv0996, (MTCI237.10), len: 358 aa. Probable conserved transmembrane protein, equivalent to AL035500|MLCL373_25 putative membrane protein from Mycobacterium leprae (342 aa), FASTA scores: (66.4% identity in 360 aa overlap). Contains possible signal sequence and other hydrophobic domains. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 1113460 885393 Rv0996 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215511.1 1112384 D 83332 CDS NP_215512.1 15608137 885386 1114293..1114724 1 NC_000962.3 Rv0997, (MTCI237.11), len: 143 aa. Hypothetical unknown protein, equivalent to AAK45276.1 from Mycobacterium tuberculosis strain CDC1551 (87 aa) but longer 56 aa.; Hypothetical protein 1114724 885386 Rv0997 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215512.1 1114293 D 83332 CDS NP_215513.1 15608138 885385 1114748..1115749 1 NC_000962.3 Rv0998, (MTCI237.12), len: 333 aa. Conserved hypothetical protein, with cyclic nucleotide-binding domain in N-terminal part and GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Possibly cyclic nucleotide-dependent protein kinase, highly similar to NP_301261.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (353 aa); and AL035500|MLCL373.38|T45457 hypothetical protein from Mycobacterium leprae (143 aa), FASTA score: (61.5% identity in 143 aa overlap). Also similar to many hypothetical proteins and cyclic-NMP-dependent protein kinases (generally at C-terminus) e.g. N-terminus of SC9B10.09|T35878 hypothetical protein from Streptomyces coelicolor (1039 aa); P05987|KAPR_DICDI camp-dependent protein kinase regulatory chain from Dictyostelium discoideum (327 aa), FASTA scores: opt: 177, E(): 0.00036,(32.0% identity in 122 aa overlap); NP_104403.1|NC_002678 hypothetical protein (contains similarity to cAMP-dependent protein kinase regulatory subunit) from Mesorhizobium loti (151 aa); etc. Contains PS00889 Cyclic nucleotide-binding domain signature 2. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1115749 885385 Rv0998 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215513.1 1114748 D 83332 CDS NP_215514.1 15608139 886043 1115767..1116525 1 NC_000962.3 Rv0999, (MTCI237.13), len: 252 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 1116525 886043 Rv0999 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215514.1 1115767 D 83332 CDS YP_177777.1 57116811 886266 complement(1116531..1117148) 1 NC_000962.3 Rv1000c, len: 205 aa. Conserved hypothetical protein, equivalent to ML0190|NP_301263.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (205 aa). Also highly similar to SC5F8.12c|CAB93740.1|AL357613 hypothetical protein from Streptomyces coelicolor (210 aa), FASTA scores: E(): 2.1e-45, (56.8% identity); 9106290|AAF84108.1|AE003963_5|NP_298588.1|NC_002488 protein described as DNA repair system specific for alkylated DNA from Xylella fastidiosa (200 aa), FASTA scores: E(): 3.4e-14, (38.55% identity); and similar in C-terminus to other hypothetical proteins. Note that replaces original Rv1000 predicted on other strand.; hypothetical protein 1117148 886266 Rv1000c Mycobacterium tuberculosis H37Rv hypothetical protein YP_177777.1 1116531 R 83332 CDS NP_215517.1 15608141 888313 1117185..1118393 1 NC_000962.3 Rv1001, (MTCI237.16), len: 402 aa. Probable arcA,arginine deiminase, similar to e.g. ARCA_PSEAE|P13981 arginine deiminase (417 aa), fasta scores: opt: 581, E(): 1.4e-31, (39.4% identity in 411 aa overlap); also similar to SAGP_STRPY|P16962 streptococcal acid glycoprotein (410 aa), FASTA scores, opt: 823, E():0, (38.3% identity in 402 aa overlap). Belongs to the arginine deiminase family.; Probable arginine deiminase ArcA (adi) (ad) (arginine dihydrolase) 1118393 arcA 888313 arcA Mycobacterium tuberculosis H37Rv Probable arginine deiminase ArcA (adi) (ad) (arginine dihydrolase) NP_215517.1 1117185 D 83332 CDS NP_215518.1 15608142 887882 complement(1118428..1119939) 1 NC_000962.3 Rv1002c, (MTCI237.17c), len: 503 aa. Conserved membrane protein. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Similar to AL132674|SCE87.05 hypothetical protein from Streptomyces coelicolor (591 aa), FASTA scores: opt: 666,E(): 0, (39.0% identity in 546 aa overlap); weakly similar and to TSCC_PSEAM|P55019 thiazide-sensitive sodium-chloride cotransporter from Pseudopleuronectes americanus (1023 aa),FASTA scores: opt: 44, E(): 4.2e-06, (22.4% identity in 326 aa overlap).; hypothetical protein 1119939 887882 Rv1002c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215518.1 1118428 R 83332 CDS NP_215519.1 15608143 887935 1120022..1120879 1 NC_000962.3 Rv1003, (MTCI237.19), len: 285 aa. Conserved protein, similar to others e.g. AL132674|SCE87.04 Streptomyces coelicolor (286 aa), FASTA scores: opt: 877,E(): 0, (53.2% identity in 280 aa overlap); and YRAL_ECOLI|P45528 hypothetical 31.3 kd protein (286 aa),FASTA scores: opt: 561, E(): 4.4e-27, (36.9% identity in 279 aa overlap).; hypothetical protein 1120879 887935 Rv1003 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215519.1 1120022 D 83332 CDS NP_215520.1 15608144 886039 complement(1120889..1122148) 1 NC_000962.3 Rv1004c, (MTCI237.20c), len: 419 aa. Probable membrane protein. Contains repetitive sequences, which have similarities with elastin, and possible N-terminal signal sequence.; Probable membrane protein 1122148 886039 Rv1004c Mycobacterium tuberculosis H37Rv Probable membrane protein NP_215520.1 1120889 R 83332 CDS NP_215521.1 15608145 888205 complement(1122222..1123598) 1 NC_000962.3 Rv1005c, (MTCI237.22c), len: 458 aa (Start-site not certain). Probable PabD, para-aminobenzoate synthase component I. Similar to PABB_ECOLI|P05041 para-aminobenzoate synthase component I from Escherichia coli (453 aa), FASTA scores: opt: 589, E(): 1.8e-27, (40.7% identity in 268 aa overlap). Similar to M. tuberculosis Rv1609, Rv3215, Rv2386c.; Probable para-aminobenzoate synthase component I PABD 1123598 pabB 888205 pabB Mycobacterium tuberculosis H37Rv Probable para-aminobenzoate synthase component I PABD NP_215521.1 1122222 R 83332 CDS NP_215522.1 15608146 888234 1123714..1125417 1 NC_000962.3 Rv1006, (MTCI237.23), len: 567 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 1125417 888234 Rv1006 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215522.1 1123714 D 83332 CDS NP_215523.1 15608147 886050 complement(1125444..1127003) 1 NC_000962.3 Rv1007c, (MTCI237.24), len: 519 aa. metS (MetG),methionyl-tRNA synthetase, similar to many e.g. SYM_BACSU|P37465 methionyl-tRNA synthetase from Bacillus subtilus (664 aa), FASTA scores: opt: 1506, E(): 0, (44.9% identity in 492 aa overlap); similar to other Mycobacterium tuberculosis tRNA synthases e.g. Rv2448c, Rv1536, Rv0041. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. Strong, to cysteinyl-tRNA synthetase.; Methionyl-tRNA synthetase MetS (MetRS) (methionine--tRNA ligase) 1127003 metS 886050 metS Mycobacterium tuberculosis H37Rv Methionyl-tRNA synthetase MetS (MetRS) (methionine--tRNA ligase) NP_215523.1 1125444 R 83332 CDS NP_215524.1 15608148 886047 1127089..1127883 1 NC_000962.3 Rv1008, (MTCI237.25), len: 264 aa. Probable tatD (alternate gene name: yjjV), deoxyribonuclease, component of twin arginine translocation protein export system (see citations below). Similar to many members of the YBL055C/YJJV family e.g. YCFH_ECOLI|P37346 Putative deoxyribonuclease ycfH (265 aa), fasta scores: opt: 487,E(): 1.4e-24, (36.7% identity in 270 aa overlap). Also similar to P37545|YABD_BACSU Putative deoxyribonuclease yabD (255 aa), FASTA scores: opt: 599, E(): 7.7e-33, (40.1% identity in 262 aa overlap). Contains PS01137 Hypothetical YBL055c/yjjV family signature 1, and PS01091 Hypothetical YBL055c/yjjV family signature 3.; Probable deoxyribonuclease TatD (YJJV protein) 1127883 tatD 886047 tatD Mycobacterium tuberculosis H37Rv Probable deoxyribonuclease TatD (YJJV protein) NP_215524.1 1127089 D 83332 CDS NP_215525.1 15608149 886048 1128091..1129179 1 NC_000962.3 Rv1009, (MTCI237.26), len: 362 aa. Probable rpfB,resuscitation-promoting factor (see citation below),similar to others from Mycobacterium tuberculosis: Rv2450c|MTV008.06c|RPFE probable resuscitation-promoting factor (172 aa), FASTA scores: E(): 1.9e-19, (42.9% identity in 147 aa overlap); Rv0867c|RPFA, Rv1884c|RPFC,and Rv2389c|RPFD. Possible lipoprotein; contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Interacts with RipA (see Hett et al., 2007). Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable resuscitation-promoting factor RpfB 1129179 rpfB 886048 rpfB Mycobacterium tuberculosis H37Rv Probable resuscitation-promoting factor RpfB NP_215525.1 1128091 D 83332 CDS NP_215526.1 15608150 888792 1129152..1130105 1 NC_000962.3 Rv1010, (MTCI237.27), len: 317 aa. Probable ksgA,dimethyladenosine transferase, similar to many e.g. KSGA_BACSU|P37468 dimethyladenosine transferase from Bacillus subtilus (292 aa), FASTA scores: opt: 524, E(): 1.5e-28, (37.2% identity in 274 aa overlap); similar to Mycobacterium tuberculosis hypothetical protein Rv1988. Contains PS01131 Ribosomal RNA adenine dimethylases signature.; Probable dimethyladenosine transferase KsgA (S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase) (16S rRNA dimethylase) (high level kasugamycin resistance protein KsgA) (kasugamycin dimethyltransferase) 1130105 ksgA 888792 ksgA Mycobacterium tuberculosis H37Rv Probable dimethyladenosine transferase KsgA (S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase) (16S rRNA dimethylase) (high level kasugamycin resistance protein KsgA) (kasugamycin dimethyltransferase) NP_215526.1 1129152 D 83332 CDS NP_215527.1 15608151 886034 1130191..1131111 1 NC_000962.3 Rv1011, (MTCI237.28, MT1040), len: 306 aa. Probable ispE, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ,similar to others e.g. Q9K3R6|ISPE_STRCO Streptomyces coelicolor (299 aa), FASTA scores: opt: 925, E(): 2.7e-49,(54.5% identity in 297 overlap); etc. Belongs to the ISPE family.; Probable 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase IspE (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase) 1131111 ispE 886034 ispE Mycobacterium tuberculosis H37Rv Probable 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase IspE (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase) NP_215527.1 1130191 D 83332 CDS NP_215528.1 15608152 886045 1131128..1131421 1 NC_000962.3 Rv1012, (MTCI237.29), len: 97 aa. Hypothetical unknown protein.; Hypothetical protein 1131421 886045 Rv1012 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215528.1 1131128 D 83332 CDS NP_215529.1 15608153 886035 1131625..1133259 1 NC_000962.3 Rv1013, (MTCI237.30-MTCY10G2.36c), len: 544 aa. Putative pks16, polyketide synthase, similar to many e.g. N-terminus of Q50857|U24657 saframycin MX1 synthetase B (1770 aa), FASTA scores: opt: 526, E(): 1.4e-25, (29.3% identity in 542 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Putative polyketide synthase Pks16 1133259 pks16 886035 pks16 Mycobacterium tuberculosis H37Rv Putative polyketide synthase Pks16 NP_215529.1 1131625 D 83332 CDS NP_215530.1 15608154 886037 complement(1133333..1133908) 1 NC_000962.3 Rv1014c, (MTCY10G2.35), len: 191 aa. Probable pth,peptidyl-tRNA hydrolase, similar to PTH_ECOLI|P23932 peptidy l-trna hydrolase from Escherichia coli (194 aa),FASTA scores: opt: 472, E(): 2.3e-25, (39.6% identity in 187 aa overlap). Belongs to the PTH family.; Probable peptidyl-tRNA hydrolase Pth 1133908 pth 886037 pth Mycobacterium tuberculosis H37Rv Probable peptidyl-tRNA hydrolase Pth NP_215530.1 1133333 R 83332 CDS NP_215531.1 15608155 885992 complement(1133921..1134568) 1 NC_000962.3 Rv1015c, (MTCY10G2.34), len: 215 aa. rplY, 50s ribosomal protein L25, similar to RL25_ECOLI|P02426 50s ribosomal protein L25 from Escherichia coli (94 aa), FASTA scores: opt: 182, E(): 2.5e-05, (38.4% identity in 86 aa overlap) and to CTC_BACSU|P14194 general stress protein from Bacillus subtilis (203 aa), FASTA scores: opt: 260,E(): 1.4e-09, (28.4% identity in 201 aa overlap). Belongs to the L25P family of ribosomal proteins.; 50S ribosomal protein L25 RplY 1134568 rplY 885992 rplY Mycobacterium tuberculosis H37Rv 50S ribosomal protein L25 RplY NP_215531.1 1133921 R 83332 CDS NP_215532.1 15608156 886066 complement(1134785..1135465) 1 NC_000962.3 Rv1016c, (MTCY10G2.33), len: 226 aa. Probable lpqT,conserved lipoprotein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0040c|Y0H3_MYCTU|P71697 Proline rich 28 kDA antigen (310 aa), FASTA scores: opt: 329, E(): 2e-17, (32.3% identity in 229 aa overlap); Rv0583c. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved lipoprotein LpqT 1135465 lpqT 886066 lpqT Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqT NP_215532.1 1134785 R 83332 CDS NP_215533.1 15608157 885993 complement(1135501..1136481) 1 NC_000962.3 Rv1017c, (MTCY10G2.32), len: 326 aa. Probable prsA,ribose-phosphate pyrophosphokinase, highly similar to others e.g. KPRS_ECOLI|P08330 ribose-phosphate pyrophosphokinase from Escherichia coli (314 aa), FASTA scores: opt: 826, E(): 0, (43.8% identity in 317 aa overlap). Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature; contains PS00144 Asparaginase / glutaminase active site signature 1. Belongs to the ribose-phosphate pyrophosphokinase family. Cofactor: both inorganic phosphate and magnesium ion are required for enzyme stability and activity (by similarity).; Probable ribose-phosphate pyrophosphokinase PrsA (phosphoribosyl pyrophosphate synthetase) (PRPP synthetase) 1136481 prsA 885993 prsA Mycobacterium tuberculosis H37Rv Probable ribose-phosphate pyrophosphokinase PrsA (phosphoribosyl pyrophosphate synthetase) (PRPP synthetase) NP_215533.1 1135501 R 83332 CDS NP_215534.1 15608158 886069 complement(1136573..1138060) 1 NC_000962.3 Rv1018c, (MTCY10G2.31), len: 495 aa. Probable glmU,UDP-n-acetylglucosamine pyrophosphorylase, similar to GCAD_BACSU|P14192 UDP-n-acetylglucosamine pyrophosphorylase (456 aa), FASTA scores: opt: 1150, E(): 0, (40.0% identity in 453 aa overlap). Similar to various Mycobacterium tuberculosis sugar-phosphate transferases e.g. Rv0334,Rv1213, Rv3264c, etc.; Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU 1138060 glmU 886069 glmU Mycobacterium tuberculosis H37Rv Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU NP_215534.1 1136573 R 83332 CDS NP_215535.1 15608159 886056 1138315..1138908 1 NC_000962.3 Rv1019, (MTCY10G2.30c), len: 197 aa. Probable transcriptional regulator, similar to many memebers of the TetR family e.g. MTCY7D11.18c (34.4% identity in 189 aa overlap). Helix turn helix motif from aa 27-48 (+5.42 SD).; Probable transcriptional regulatory protein (probably TetR-family) 1138908 886056 Rv1019 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR-family) NP_215535.1 1138315 D 83332 CDS NP_215536.1 15608160 886077 1138967..1142671 1 NC_000962.3 Rv1020, (MTCY10G2.29c), len: 1234 aa. Probable mfd (alternate gene name: trcF), transcription-repair coupling factor (see citation below), similar to many e.g. MFD_ECOLI|P30958 transcription-repair coupling factor from Escherichia coli (1148 aa), FASTA scores: opt: 1900, E(): 0, (37.9% identity in 1107 aa overlap); similar to M. tuberculosis Rv2973c and Rv1633. Contains PS00017 ATP/GTP-binding site motif A (P-loop). In the N-terminal section; belongs to the UVRB family. In the C-terminal section; belongs to the helicase family. RECG subfamily.; Probable transcription-repair coupling factor Mfd (TRCF) 1142671 mfd 886077 mfd Mycobacterium tuberculosis H37Rv Probable transcription-repair coupling factor Mfd (TRCF) NP_215536.1 1138967 D 83332 CDS NP_215537.1 15608161 886052 1142671..1143648 1 NC_000962.3 Rv1021, (MTCY10G2.28c), len: 325 aa. Conserved protein, similar to YBL1_STRCI|P33653 hypothetical 26.1 kDa protein from Streptomyces cacaoi (242 aa), FASTA scores: opt: 493, E(): 1.1e-23, (42.9% identity in 238 aa overlap).; hypothetical protein 1143648 886052 Rv1021 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215537.1 1142671 D 83332 CDS NP_215538.1 15608162 886076 1143736..1144467 1 NC_000962.3 Rv1022, (MTCY10G2.27c), len: 243 aa. Probable lpqU conserved lipoprotein. Similar to Mycobacterium tuberculosis hypothetical protein Rv1230c|MTV006.02C, FASTA scores: E(): 2.8e-18, (37.9% identity in 240 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA scores: opt: 474,E(): 2.7e-21, (42.2% identity in 211 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LpqU 1144467 lpqU 886076 lpqU Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqU NP_215538.1 1143736 D 83332 CDS NP_215539.1 15608163 886062 1144564..1145853 1 NC_000962.3 Rv1023, (MTCY10G2.26c), len: 429 aa. Probable eno,enolase, highly similar to others e.g. ENO_ECOLI|P08324 enolase from Escherichia coli (431 aa), FASTA scores: opt: 1487, E(): 0, (55.5% identity in 422 aa overlap); etc. Magnesium is required for catalysis and for stabilizing the dimer. Belongs to the enolase family.; Probable enolase Eno 1145853 eno 886062 eno Mycobacterium tuberculosis H37Rv Probable enolase Eno NP_215539.1 1144564 D 83332 CDS NP_215540.1 15608164 886059 1145858..1146544 1 NC_000962.3 Rv1024, (MTCY10G2.25c), len: 228 aa. Possible conserved membrane protein, with a hydrophobic region from aa 83-101. Equivalent to ML0256|NP_301311.1|NC_002677 possible conserved membrane protein from Mycobacterium leprae (227 aa), S&W scores: 178, E()= 2e-72, Identities: 145/203 (71%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved membrane protein 1146544 886059 Rv1024 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_215540.1 1145858 D 83332 CDS NP_215541.1 15608165 886042 1146561..1147028 1 NC_000962.3 Rv1025, (MTCY10G2.24c), len: 155 aa. Conserved protein, similar to hypothetical protein AE001768|AE001768_4 Thermotoga maritima (170 aa) FASTA scores: opt: 254, E(): 9.5e-10, (35.7% identity in 143 aa overlap).; hypothetical protein 1147028 886042 Rv1025 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215541.1 1146561 D 83332 CDS NP_215542.1 15608166 886089 1147019..1147978 1 NC_000962.3 Rv1026, (MTCY10G2.23c), len: 319 aa. Conserved protein. Similar to GPPA_ECOLI|P25552 guanosine-5'-triphosphate,3'-diphosphate pyrophoshatase from Escherichia coli (494 aa), FASTA scores: opt: 281,E(): 3.2e-11, (30.6% identity in 291 aa overlap). Equivalent to AL023514|MLCB4.02 hypothetical protein from Mycobacterium leprae (317 aa) (77.9% identity in 321 aa overlap).; hypothetical protein 1147978 886089 Rv1026 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215542.1 1147019 D 83332 CDS NP_215543.1 15608167 886046 complement(1148427..1149107) 1 NC_000962.3 Rv1027c, (MTCY10G2.22), len: 226 aa. Probable KdpE,transcriptional regulatory protein, similar to others e.g. KDPE_ECOLI|P21866 kdp operon transcriptional regulatory protein from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 691, E(): 0, (47.8% identity in 224 aa overlap); AL021530|SC2E9.13 from Streptomyces coelicolor (227 aa), FASTA scores: opt: 981, E(): 0, (66.4% identity in 226 aa overlap); etc.; Probable transcriptional regulatory protein KdpE 1149107 kdpE 886046 kdpE Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein KdpE NP_215543.1 1148427 R 83332 CDS NP_215544.1 15608168 886084 complement(1149104..1151686) 1 NC_000962.3 Rv1028c, (MTCY10G2.21), len: 860 aa. Probable kdpD,sensor protein, similar to others e.g. KDPD_ECOLI|P21865 sensor protein from Escherichia coli strain K12 (894 aa),FASTA scores: opt: 1041, E(): 0, (32.3% identity in 888 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to universal stress protein family.; Probable sensor protein KdpD 1151686 kdpD 886084 kdpD Mycobacterium tuberculosis H37Rv Probable sensor protein KdpD NP_215544.1 1149104 R 83332 CDS YP_177636.1 57116812 3205056 1151920..1152012 1 NC_000962.3 Rv1028A, len: 30 aa. Probable kdpF, membrane protein, showing similarity with P36937|KDPF_ECOLI|B0698.1 protein KDPF from Escherichia coli strain K12 (see citation below) (27% identity); and KdpF protein from Streptomyces coelicolor (51% identity).; Probable membrane protein KdpF 1152012 kdpF 3205056 kdpF Mycobacterium tuberculosis H37Rv Probable membrane protein KdpF YP_177636.1 1151920 D 83332 CDS NP_215545.1 15608169 887414 1152012..1153727 1 NC_000962.3 Rv1029, (MTCY10G2.20c), len: 571 aa. Probable kdpA,potassium-transporting ATPase a chain (transmembrane protein), similar to others e.g. ATKA_ECOLI|P03959|KDPA|B0698 potassium-transporting ATPase A chain from Escherichia coli strain K12 (557 aa), FASTA scores: opt: 1763, E(): 0, (50.4% identity in 569 aa overlap); etc. Belongs to the KdpA family.; Probable potassium-transporting ATPase a chain KdpA (potassium-translocating ATPase a chain) (ATP phosphohydrolase [potassium-transporting] a chain) (potassium binding and translocating subunit A) 1153727 kdpA 887414 kdpA Mycobacterium tuberculosis H37Rv Probable potassium-transporting ATPase a chain KdpA (potassium-translocating ATPase a chain) (ATP phosphohydrolase [potassium-transporting] a chain) (potassium binding and translocating subunit A) NP_215545.1 1152012 D 83332 CDS NP_215546.1 15608170 887343 1153724..1155853 1 NC_000962.3 Rv1030, (MTCY10G2.19c), len: 709 aa. Probable kdpB,potassium-transporting P-type ATPase B chain (transmembrane protein), similar to others e.g. ATKB_ECOLI|P03960 potassium-transporting ATPase B chain from Escherichia coli strain K12 (682 aa), FASTA scores: opt: 1481, E(): 0,(63.4% identity in 686 aa overlap); etc. Very similar to AL078610|SCH35.47 H+/K+-exchanging ATPase chain B from Streptomyces coelicolor (707 aa), FASTA scores: opt: 2731,E(): 0, (71.6% identity in 676 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site.; Probable potassium-transporting P-type ATPase B chain KdpB (potassium-translocating ATPase B chain) (ATP phosphohydrolase [potassium-transporting] B chain) (potassium binding and translocating subunit B) 1155853 kdpB 887343 kdpB Mycobacterium tuberculosis H37Rv Probable potassium-transporting P-type ATPase B chain KdpB (potassium-translocating ATPase B chain) (ATP phosphohydrolase [potassium-transporting] B chain) (potassium binding and translocating subunit B) NP_215546.1 1153724 D 83332 CDS NP_215547.1 15608171 886008 1155853..1156422 1 NC_000962.3 Rv1031, (MTCY10G2.18c), len: 189 aa. Probable kdpC,potassium-transporting ATPase C chain (membrane protein) ,similar to others e.g. ATKC_ECOLI|P03961 potassium-transporting ATPase C chain from Escherichia coli strain K12 (190 aa), FASTA scores: opt: 475, E(): 3.1e-24,(45.7% identity in 186 aa overlap); etc. Belongs to the KdpC family.; Probable potassium-transporting ATPase C chain KdpC (potassium-translocating ATPase C chain) (ATP phosphohydrolase [potassium-transporting] C chain) (potassium binding and translocating subunit C) 1156422 kdpC 886008 kdpC Mycobacterium tuberculosis H37Rv Probable potassium-transporting ATPase C chain KdpC (potassium-translocating ATPase C chain) (ATP phosphohydrolase [potassium-transporting] C chain) (potassium binding and translocating subunit C) NP_215547.1 1155853 D 83332 CDS NP_215548.1 15608172 887790 complement(1156426..1157955) 1 NC_000962.3 Rv1032c, (MTCY10G2.17), len: 509 aa. TrcS, two component sensor histidine kinase protein (see citations below), similar to YV16_MYCLE|P54883 probable sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA scores: opt: 392, E(): 3.8e-18, (31.7% identity in 334 aa overlap). Note that in vitro autophosphorylation of TrcS requires the presence of Mn2+ or Ca2+ as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+.; Two component sensor histidine kinase TrcS 1157955 trcS 887790 trcS Mycobacterium tuberculosis H37Rv Two component sensor histidine kinase TrcS NP_215548.1 1156426 R 83332 CDS NP_215549.1 15608173 887957 complement(1157963..1158736) 1 NC_000962.3 Rv1033c, (MTCY10G2.16), len: 257 aa. TrcR,two-component regulatory protein (see citations below),similar to Q50825 two component response regulator from Mycobacterium tuberculosis (234 aa), FASTA scores: opt: 628, E(): 0, (46.0% identity in 226 aa overlap). Note that in vitro autophosphorylation of TrcS requires the presence of Mn2+or Ca2+as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+.; Two component transcriptional regulator TrcR 1158736 trcR 887957 trcR Mycobacterium tuberculosis H37Rv Two component transcriptional regulator TrcR NP_215549.1 1157963 R 83332 CDS NP_215550.1 15608174 886010 complement(1158918..1159307) 1 NC_000962.3 Rv1034c, (MTCY10G2.15), len: 129 aa. Probable IS1560 transposase fragment, similar to part of Rv3387|E1202305|MTV004.45 (225 aa) (65.1% identity in 129 aa overlap).; Probable transposase (fragment) 1159307 886010 Rv1034c Mycobacterium tuberculosis H37Rv Probable transposase (fragment) NP_215550.1 1158918 R 83332 CDS NP_215551.1 15608175 888206 complement(1159375..1160061) 1 NC_000962.3 Rv1035c, (MTCY10G2.14), len: 228 aa. Probable IS1560 transposase fragment, similar to parts of Rv3387|E1202305|MTV004.45 (225 aa) (47.8% identity in 67 aa overlap) and Rv3386|E1202304|MTV004.44 (234 aa) (55.1% identity in 127 aa overlap).; Probable transposase (fragment) 1160061 888206 Rv1035c Mycobacterium tuberculosis H37Rv Probable transposase (fragment) NP_215551.1 1159375 R 83332 CDS NP_215552.1 15608176 888227 complement(1160095..1160433) 1 NC_000962.3 Rv1036c, (MTCY10G2.13), len: 112 aa. Probable IS1560 transposase fragment, similar to part of Rv3386|E1202304|MTV004.44 (234 aa) (82.8% identity in 87 aa overlap).; Probable IS1560 transposase (fragment) 1160433 888227 Rv1036c Mycobacterium tuberculosis H37Rv Probable IS1560 transposase (fragment) NP_215552.1 1160095 R 83332 CDS NP_215553.1 15608177 888299 complement(1160544..1160828) 1 NC_000962.3 Rv1037c, (MTCY10G2.12), len: 94 aa. EsxI, ESAT-6 like protein (see citations below), highly similar to Q49946|ES6X_MYCLE|U1756D putative ESAT-6 like protein X from Mycobacterium leprae (95 aa), FASTA scores: opt: 409,E(): 6.3e-23, (64.15% identity in 92 aa overlap); Rv3619c,Rv1198, Rv2346c, etc from Mycobacterium tuberculosis. Strictly identical to P96364|ES61_MYCTU|Rv3619c|MTCY15C10.33|MTCY07H7B.03|MT3721 putative ESAT-6 like protein 1 (94 aa). Belongs to the ESAT6 family.; Putative ESAT-6 like protein EsxI (ESAT-6 like protein 1) 1160828 esxI 888299 esxI Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxI (ESAT-6 like protein 1) NP_215553.1 1160544 R 83332 CDS NP_215554.1 15608178 888372 complement(1160855..1161151) 1 NC_000962.3 Rv1038c, (MT1067, MTCY10G2.11), len: 98 aa. EsxJ,ESAT-6 like protein (see Gey Van Pittius et al., 2001),similar to Q49945|U1756C, Mycobacterium leprae (100 aa),FASTA scores: opt: 375, E(): 7.7e-21, (58.3% identity in 96 aa overlap). Member of M. tuberculosis hypothetical QILSS protein family with Rv1197, Rv1792, Rv2347c and Rv3620c. Belongs to the ESAT6 family.; ESAT-6 like protein EsxJ (ESAT-6 like protein 2) 1161151 esxJ 888372 esxJ Mycobacterium tuberculosis H37Rv ESAT-6 like protein EsxJ (ESAT-6 like protein 2) NP_215554.1 1160855 R 83332 CDS YP_177778.1 57116813 888477 complement(1161297..1162472) 1 NC_000962.3 Rv1039c, (MTCY10G2.10), len: 391 aa. PPE15, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv2768c|AL008967|MTV002_33 Mycobacterium tuberculosis H37Rv (394 aa), FASTA scores: opt: 1721, E(): 0, (70.4% identity in 398 aa overlap).; PPE family protein PPE15 1162472 PPE15 888477 PPE15 Mycobacterium tuberculosis H37Rv PPE family protein PPE15 YP_177778.1 1161297 R 83332 CDS YP_177779.1 57116814 888533 complement(1162549..1163376) 1 NC_000962.3 Rv1040c, (MTCY10G2.09), len: 275 aa. PE8, Member of the Mycobacterium tuberculosis PE family (see citation below), most similar to AL008967|MTV002_34 Mycobacterium tuberculosis H37Rv (275 aa), FASTA scores: opt: 1111, E(): 0, (68.6% identity in 283 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE family protein PE8 1163376 PE8 888533 PE8 Mycobacterium tuberculosis H37Rv PE family protein PE8 YP_177779.1 1162549 R 83332 CDS NP_215557.1 15608181 888546 complement(1164572..1165435) 1 NC_000962.3 Rv1041c, (MTCY10G2.08), len: 287 aa. Probable is like-2 transposase, overlaps MTCY10G2.07. Similar to Q00430|X53945 insertion element IS869 hypothetical protein from Agrobacterium tumefaciens (186 aa), FASTA scores: opt: 173, E(): 0.00016, (40.9% identity in 176 aa overlap). Similar to Rv1150, C-terminal part of transposase of putative Mycobacterium tuberculosis is like-1. MTCY10G2.07 and MTCY10G2.08 are frameshifted with respect to Mycobacterium tuberculosis Q50761 transposase, the 10G2 cosmid sequence appears to be correct. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable is like-2 transposase 1165435 888546 Rv1041c Mycobacterium tuberculosis H37Rv Probable is like-2 transposase NP_215557.1 1164572 R 83332 CDS NP_215558.1 15608182 888607 complement(1165092..1165499) 1 NC_000962.3 Rv1042c, (MTCY10G2.07), len: 135 aa. Probable is like-2 transposase, similar to Q50761 transposase from Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1149. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable is like-2 transposase 1165499 888607 Rv1042c Mycobacterium tuberculosis H37Rv Probable is like-2 transposase NP_215558.1 1165092 R 83332 CDS NP_215559.1 15608183 888634 complement(1165781..1166806) 1 NC_000962.3 Rv1043c, (MTCY10G2.06), len: 341 aa. Conserved hypothetical protein similar to AL096872|SC5F7.08 putative lipoate-protein ligase from Streptomyces coelicolor (362 aa), FASTA scores: opt: 206, E(): 1.4e-05, (30.3% identity in 201 aa overlap). Weak similarity to P39668|YYXA_BACSU hypothetical protease from Bacillus subtitis (400 aa),FASTA scores: opt: 159, E(): 0.013, (27.1% identity in 210 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 1166806 888634 Rv1043c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215559.1 1165781 R 83332 CDS NP_215560.1 15608184 888712 1167053..1167676 1 NC_000962.3 Rv1044, (MTCY10G2.05c), len: 207 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical protein MTCY06G11.02C|P96837 (289 aa), fasta scores: E(): 8.9e-06, (30.7% identity in 150 aa overlap). Some similarity to U36837|LLU36837_1 Lactococcus lactis plasmid pNP40 (287 aa), FASTA scores: opt: 147, E (): 0.0087, (29.7% identity in 91 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 1167676 888712 Rv1044 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215560.1 1167053 D 83332 CDS NP_215561.1 15608185 888783 1167673..1168554 1 NC_000962.3 Rv1045, (MTCY10G2.04c), len: 293 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 1168554 888783 Rv1045 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215561.1 1167673 D 83332 CDS NP_215562.2 57116815 886071 complement(1168704..1169228) 1 NC_000962.3 Rv1046c, (MTCY10G2.03), len: 174 aa. Hypothetical unknown protein. Start changed since first submission (-65 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 1169228 886071 Rv1046c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215562.2 1168704 R 83332 CDS NP_215563.1 15608187 886060 1169423..1170670 1 NC_000962.3 Rv1047, (MTCY10G2.02c), len: 415 aa. IS1081 transposase, most similar to TRA1_MYCBO|P35882 transposase for insertion sequence element (415 aa), FASTA scores: opt: 2675, E(): 0, (99.8% identity in 415 aa overlap). Contains PS01007 Transposases, Mutator family, signature. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase 1170670 886060 Rv1047 Mycobacterium tuberculosis H37Rv Probable transposase NP_215563.1 1169423 D 83332 CDS NP_215564.1 15608188 885295 complement(1171038..1172153) 1 NC_000962.3 Rv1048c, (MTV017.01c-MTCY10G2.01), len: 371 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 1172153 885295 Rv1048c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215564.1 1171038 R 83332 CDS NP_215565.1 15608189 886091 1172386..1172832 1 NC_000962.3 Rv1049, (MTV017.02), len: 148 aa. Probable transcriptional repressor protein, similar to many e.g. P74870 negative regulator of EMR locus EMR from Salmonella typhimurium (149 aa), FASTA scores: opt: 146, E(): 0.0011,(31.6% identity in 95 aa overlap). Contains probable helix-turn-helix motif at aa 58-79 (Score 1495, +4.28 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transcriptional repressor protein 1172832 886091 Rv1049 Mycobacterium tuberculosis H37Rv Probable transcriptional repressor protein NP_215565.1 1172386 D 83332 CDS NP_215566.1 15608190 887146 1172881..1173786 1 NC_000962.3 Rv1050, (MTV017.03), len: 301 aa. Probable oxidoreductase similar to many e.g. Rv1543|MTCY48.22C|Q10783 putative oxidoreductase CY48.22C (341 aa), FASTA scores: opt: 462, E(): 3e-22, (33.6% identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable oxidoreductase 1173786 887146 Rv1050 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_215566.1 1172881 D 83332 CDS NP_215567.1 15608191 887142 complement(1173945..1174700) 1 NC_000962.3 Rv1051c, (MTV017.04c), len: 251 aa. Conserved hypothetical protein, similar to LLU36837|U36837.1 protein encoded by Lactococcus lactis plasmid pNP40 (298 aa), FASTA scores: opt: 194, E(): 3.5e-06, (30.3% identity in 155 aa overlap). Contains possible helix-turn-helix motif at aa 197-218 (Score 1097, +2.92 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 1174700 887142 Rv1051c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215567.1 1173945 R 83332 CDS NP_215568.1 15608192 888441 1175723..1176112 1 NC_000962.3 Rv1052, (MTV017.05), len: 129 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 1176112 888441 Rv1052 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215568.1 1175723 D 83332 CDS NP_215569.1 15608193 887141 complement(1176011..1176286) 1 NC_000962.3 Rv1053c, (MTV017.06c), len: 91 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 1176286 887141 Rv1053c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215569.1 1176011 R 83332 CDS NP_215570.2 57116816 887139 1176928..1177242 1 NC_000962.3 Rv1054, (MTV017.07), len: 104 aa. Probable integrase (fragment), similar to Rv2309c|MTCY3G12_25|Z79702 hypothetical protein (shows similarity to integrases) from Mycobacterium tuberculosis (151 aa), FASTA scores: opt: 273, E(): 8.8e-13, (64.7% identity in 68 aa overlap); and to L39071|MSGINT_1 integrase from Mycobacterium paratuberculosis (191 aa), FASTA scores: opt: 105, E(): 0.9, (31.8% identity in 85 aaoverlap). This ORF continues in another frame as Rv1055|MTV017.08 but no error can be found to account for frameshift. Length extended since first submission (+36 aa). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable integrase (fragment) 1177242 887139 Rv1054 Mycobacterium tuberculosis H37Rv Probable integrase (fragment) NP_215570.2 1176928 D 83332 CDS NP_215571.2 57116817 887138 1177239..1177373 1 NC_000962.3 Rv1055, (MTV017.08), len: 44 aa. Possible integrase (fragment); first 49 aa similar to Rv2309c|MTCY3G12_25|Z79702 hypothetical protein (shows similarity to integrases) from Mycobacterium tuberculosis (151 aa), FASTA scores: opt: 291, E(): 2.2e-16, (74.3% identity in 70 aa overlap); and to L39071|MSGINT_1 integrase from Mycobacterium paratuberculosis (191 aa),FASTA scores: opt: 146, E(): 8.3e-05, (52.1% identity in 48 aa overlap); and to many other integrases or transposases. Shortened since first submission (-34 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible integrase (fragment) 1177373 887138 Rv1055 Mycobacterium tuberculosis H37Rv Possible integrase (fragment) NP_215571.2 1177239 D 83332 CDS NP_215572.1 15608196 887147 1177628..1178392 1 NC_000962.3 Rv1056, (MTV017.09), len: 254 aa. Conserved protein,some similarity in C-terminal region of Rv0140|MTCI5.14|Z92770 Mycobacterium tuberculosis (126 aa),FASTA scores: opt: 254, E(): 1.2e-10, (43.4% identity in 106 aa overlap); and to Rv1670. C-terminal region is similar to AL035569|SC8D9.02 hypothetical protein from Streptomyces coelicolor (113 aa), FASTA scores: opt: 282,E(): 4.5e-12, (48.0% identity in 100 aa overlap).; hypothetical protein 1178392 887147 Rv1056 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215572.1 1177628 D 83332 CDS NP_215573.1 15608197 887135 1179396..1180577 1 NC_000962.3 Rv1057, (MTV017.10), len: 393 aa. Conserved hypothetical protein, some similarity to X84710|MMSAG_1 surface antigen of Methanosarcina mazeii (491 aa), FASTA scores: opt: 363, E():6.2e-15, (31.3% identity in 294 aa overlap). Regulated by MprA (Rv0981) under physiological conditions and environmental stress (SDS and Triton X-100) (See He et al., 2006).; hypothetical protein 1180577 887135 Rv1057 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215573.1 1179396 D 83332 CDS NP_215574.1 15608198 887133 1180684..1182315 1 NC_000962.3 Rv1058, (MTV017.11), len: 543 aa. Probable fadD14,medium-chain fatty-acid-CoA synthetase, highly similar to many e.g. CAC32346.1|AL583945 putative fatty acid CoA ligase from Streptomyces coelicolor (558 aa); N-terminus of NP_419738.1|NC_002696 medium-chain-fatty-acid--CoA ligase from Caulobacter crescentus (1006 aa); Q00594|ALKK_PSEOL medium-chain-fatty-acid--CoA ligase from Pseudomonas oleovorans (546 aa), FASTA scores: opt: 1468, E(): 0,(41.1% identity in 538 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Probable medium chain fatty-acid-CoA ligase FadD14 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 1182315 fadD14 887133 fadD14 Mycobacterium tuberculosis H37Rv Probable medium chain fatty-acid-CoA ligase FadD14 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_215574.1 1180684 D 83332 CDS NP_215575.1 15608199 886015 1182391..1183455 1 NC_000962.3 Rv1059, (MTV017.12), len: 354 aa. Conserved protein,similar to Rv0926c|MTCY21C12.20c hypothetical protein from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 338, E(): 1.4e-14, (33.1% identity in 363 aa overlap).; hypothetical protein 1183455 886015 Rv1059 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215575.1 1182391 D 83332 CDS NP_215576.1 15608200 887130 1183508..1183981 1 NC_000962.3 Rv1060, (MTV017.13), len: 157 aa. Unknown protein.; hypothetical protein 1183981 887130 Rv1060 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215576.1 1183508 D 83332 CDS NP_215577.1 15608201 887136 1184015..1184878 1 NC_000962.3 Rv1061, (MTV017.14), len: 287 aa. Conserved protein,similar to hypothetical proteins from various bacteria e.g. D64002|SYCSLRD_75 Synechocystis sp. PCC6803 (304 aa),FASTA scores: opt: 245, E():1.2e-09, (27.1% identity in 258 aa overlap).; hypothetical protein 1184878 887136 Rv1061 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215577.1 1184015 D 83332 CDS NP_215578.1 15608202 887129 1184883..1185740 1 NC_000962.3 Rv1062, (MTV017.15), len: 285 aa. Conserved hypothetical protein, some similarity to AL079356|SC6G9_10 hypothetical protein in Streptomyces coelicolor (289 aa),FASTA scores: opt: 556, E(): 1.2e-27, (39.0% identity in 287 aa overlap), and Z99111|BSUB0008_176 Bacillus subtilis (260aa), FASTA scores: opt: 163, E(): 0.0013, (27.4% identity in 179aa overlap).; hypothetical protein 1185740 887129 Rv1062 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215578.1 1184883 D 83332 CDS NP_215579.1 15608203 887128 complement(1185741..1186823) 1 NC_000962.3 Rv1063c, (MTV017.16c), len: 360 aa. Conserved hypothetical protein, similar to P37053|YCHK_ECOLI hypothetical protein from Escherichia coli (314 aa), FASTA scores: opt: 487, E(): 7.2e-23, (32.7% identity in 321 aa overlap). Also partially similar to Rv3239c|MTCY20B11.14c. Belongs to the UPF0028 (SWS) family.; hypothetical protein 1186823 887128 Rv1063c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215579.1 1185741 R 83332 CDS NP_215580.1 15608204 887126 complement(1186904..1187323) 1 NC_000962.3 Rv1064c, (MTV017.17c), len: 139 aa. Possible lipoprotein LpqV. Has N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Possible lipoprotein LpqV 1187323 lpqV 887126 lpqV Mycobacterium tuberculosis H37Rv Possible lipoprotein LpqV NP_215580.1 1186904 R 83332 CDS NP_215581.1 15608205 887125 1187435..1188001 1 NC_000962.3 Rv1065, (MTV017.18), len: 188 aa. Conserved hypothetical protein, some similarity to AL0209|SC4H8_11 hypothetical protein from Streptomyces coelicolor (182 aa),FASTA scores: opt: 156, E(): 0.0011, (31.3% identity in 195 aa overlap).; hypothetical protein 1188001 887125 Rv1065 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215581.1 1187435 D 83332 CDS NP_215582.1 15608206 887127 1187998..1188393 1 NC_000962.3 Rv1066, (MTV017.19), len: 131 aa. Conserved hypothetical protein, strong similarity to AL0209|SC4H8.10 hypothetical protein from Streptomyces coelicolor (132 aa),FASTA scores: opt: 429, E(): 5.2e-23, (57.1% identity in 119 aa overlap).; hypothetical protein 1188393 887127 Rv1066 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215582.1 1187998 D 83332 CDS YP_177780.1 57116818 887122 complement(1188421..1190424) 1 NC_000962.3 Rv1067c, (MTV017.20c), len: 667 aa. PE_PGRS19,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to Rv3388|MTV004.46 M. tuberculosis (731 aa), FASTA scores: opt: 2227, E(): 0, (55.6% identity in 710 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1, probably fortuitous. Predicted to be an outer membrane protein (See Song et al., 2008).; PE-PGRS family protein PE_PGRS19 1190424 PE_PGRS19 887122 PE_PGRS19 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS19 YP_177780.1 1188421 R 83332 CDS YP_177781.1 57116819 887123 complement(1190757..1192148) 1 NC_000962.3 Rv1068c, (MTV017.21c), len: 463 aa. PE_PGRS20,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to AL021897|MTV017_19 Mycobacterium tuberculosis H37Rv (667 aa), FASTA scores: opt: 1875, E(): 0, (55.0% identity in 667 aa overlap).; PE-PGRS family protein PE_PGRS20 1192148 PE_PGRS20 887123 PE_PGRS20 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS20 YP_177781.1 1190757 R 83332 CDS NP_215585.1 15608209 887120 complement(1192510..1194273) 1 NC_000962.3 Rv1069c, (MTV017.22c), len: 587 aa. Conserved protein, hydrophobic regions in N-terminal domain. Similar in part to O07136|B1306.04C B1306.04c protein from Mycobacterium leprae (89 aa), FASTA scores: opt: 229, E(): 1.3e-07, (54.2% identity in 72 aa overlap).; hypothetical protein 1194273 887120 Rv1069c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215585.1 1192510 R 83332 CDS NP_215586.1 15608210 887117 complement(1194270..1195043) 1 NC_000962.3 Rv1070c, (MTV017.23c), len: 257 aa. Probable echA8,enoyl-CoA hydratase, equivalent to O07137|B1306.05c putative enoyl-CoA hydratase/isomerase from Mycobacterium leprae (257 aa), FASTA scores: opt: 1417, E(): 0, (86.4% identity in 257 aa overlap). Also highly similar to others e.g. NP_106219.1|NC_002678 enoyl CoA hydratase from Mesorhizobium loti (257 aa); L39265|RHMRPST_2 enoyl-CoA hydratase from Rhizobium melilotii (257 aa), FASTA scores: opt: 1100, E(): 0, (66.9% identity in 257 aa overlap); AAK18173.1|AF290950_5|AF290950|FadB1x enoyl-CoA hydratase from Pseudomonas putida (257 aa); etc. Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family.; Probable enoyl-CoA hydratase EchA8 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 1195043 echA8 887117 echA8 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA8 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215586.1 1194270 R 83332 CDS NP_215587.1 15608211 887116 complement(1195055..1196092) 1 NC_000962.3 Rv1071c, (MTV017.24c), len: 345 aa. Possible echA9,enoyl-CoA hydratase, equivalent to Y13803|B1306.06c putative enoyl-CoA hydratase/isomerase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1799, E(): 0, (77.7% identity in 345 aa overlap). Also similar to many eukaryotic and prokaryotic enoyl-CoA hydratases e.g. NP_437984.1|NC_003078 putative enoyl-CoA hydratase protein from Sinorhizobium meliloti (356 aa); NP_420165.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (350 aa); Q19278 protein similar to enoyl-CoA hydratases from Caenorhabditis elegans (386),FASTA scores: opt: 787, E(): 0, (38.5% identity in 348 aa overlap); etc.; Possible enoyl-CoA hydratase EchA9 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 1196092 echA9 887116 echA9 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase EchA9 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215587.1 1195055 R 83332 CDS NP_215588.1 15608212 887114 1196279..1197115 1 NC_000962.3 Rv1072, (MTV017.25), len: 278 aa. Probable conserved transmembrane protein, equivalent to O07139|B1306.07|Y13803 Protein B1306.07 from Mycobacterium leprae (220 aa), FASTA scores: opt:1032, E(): 0, (75.0% identity in 220 aa overlap); and at the C-terminal end to Q50056|U1740D Mycobacterium leprae (96 aa), FASTA scores: opt: 381, E(): 1.2e-18, (71.6% identity in 81 aa overlap). Similar to Q54192|M80628|STMBLDA_1 transfer RNA-LEU (BLDA) gene and ORF from Streptomyces griseus (293 aa), FASTA scores: opt:558, E(): 4.7e-30, (41.5% identity in 299 aa overlap).; Probable conserved transmembrane protein 1197115 887114 Rv1072 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_215588.1 1196279 D 83332 CDS NP_215589.1 15608213 887132 1197231..1198082 1 NC_000962.3 Rv1073, (MTV017.26), len: 283 aa. Conserved hypothetical protein, similar to several hypothetical mycobacterial proteins e.g. Rv1482c|Z79701|MTCY277.03 Mycobacterium tuberculosis (339 aa), FASTA scores: opt: 810, E(): 0, (47.4% identity in 272 aa overlap); Rv3555c|Z92774|MTCY6G11_2 Mycobacterium tuberculosis (289 aa), FASTA scores: opt: 704, E(): 0, (44.4% identity in 259 aa overlap); and Rv3517, etc., and GIR10|AF002133_10 Mycobacterium avium strain GIR10 (346 aa), FASTA scores: opt: 802, E(): 0, (48.1% identity in 270 aa overlap).; hypothetical protein 1198082 887132 Rv1073 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215589.1 1197231 D 83332 CDS NP_215590.1 15608214 887113 complement(1198156..1199373) 1 NC_000962.3 Rv1074c, (MTV017.27c), len: 405 aa. Probable fadA3,beta-ketoacyl CoA thiolase, highly similar to many involved in beta-oxidation e.g. CAB89028.1|AL353870 beta-ketoadipyl-CoA thiolase from Streptomyces coelicolor (395 aa); P77525|PAAJ_ECOLI probable beta-ketoadipyl CoA thiolase from Escherichia coli (401 aa), FASTA scores: opt: 1034, E(): 5.4e-56, (43.5% identity in 416 aa overlap) and X97452 acetyl-CoA acetyltransferase (thiolase) from Escherichia coli (401 aa), FASTA scores: opt: 1043, E(): 0,(43.4% identity in 415 aa overlap); Q43935|CATF_ACICA beta-ketoadipyl CoA thiolase from Acinetobacter calcoaceticus (401 aa), FASTA scores: opt: 992, E(): 0,(41.5% identity in 415 aa overlap); etc. Contains PS00737 Thiolases signature 2, and PS00445 FGGY family of carbohydrate kinases signature 2, although this is probably fortuitous. Belongs to the thiolase family.; Probable beta-ketoacyl CoA thiolase FadA3 1199373 fadA3 887113 fadA3 Mycobacterium tuberculosis H37Rv Probable beta-ketoacyl CoA thiolase FadA3 NP_215590.1 1198156 R 83332 CDS NP_215591.1 15608215 887110 complement(1199426..1200370) 1 NC_000962.3 Rv1075c, (MTV017.28c), len: 314 aa. Possibly exported protein, as it contains a N-terminal signal sequence, hydrophobic domain from aa 7-25. Similar to U15183|MLU15183_2 Mycobacterium leprae cosmid B1740 (106 aa), FASTA scores: opt: 207, E(): 1.6e-06, (42.6% identity in 101 aa overlap). Also weak similarity to many glyceraldehyde-3-phosphate dehydrogenases e.g. Q41595|G3PC_TAXBA Taxus baccata (340 aa), FASTA scores: opt: 147, E(): 0.027, (27.5% identity in 189 aa overlap).; Conserved exported protein 1200370 887110 Rv1075c Mycobacterium tuberculosis H37Rv Conserved exported protein NP_215591.1 1199426 R 83332 CDS NP_215592.1 15608216 887112 1200767..1201660 1 NC_000962.3 Rv1076, (MTV017.29), len: 297 aa. Possible lipU,lipase, very similar to several Mycobacterium tuberculosis proteins e.g. Z95390|Rv3487c|MTCY13E12.41c (277 aa), FASTA scores: opt: 1225, E(): 0, (76.0% identity in 246 aa overlap); Rv1426c, etc. Also similar to esterases and lipases of around 300 aa e.g. Q44087 esterase precursor from Acinetobacter lwoffii esterase (303), FASTA scores: opt: 427, E(): 1.9e-21, (32.5% identity in 280 aa overlap). Equivalent to AL035159|MLCB1450 _7 Mycobacterium leprae (335 aa), FASTA scores: opt: 1588, E(): 0, (79.7% identity in 296 aa overlap).; Possible lipase LipU 1201660 lipU 887112 lipU Mycobacterium tuberculosis H37Rv Possible lipase LipU NP_215592.1 1200767 D 83332 CDS YP_177782.1 57116820 887108 1201717..1203111 1 NC_000962.3 Rv1077, (MTV017.30), len: 464 aa. Probable cbs (previously cysM2), cystathionine beta-synthase, similar throughout its length to many eukaryotic cystathionine beta-synthases e.g. P32232|CBS_RAT cystathionine beta-synthase (560 aa), FASTA scores: opt: 951, E(): 0,(40.2% identity in 450 aa overlap); also similar in N-terminal domain (aa 1 - 330) to Rv2334|MTCY98.03 CysK Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 855, E(): 0, (46.8% identity in 314 overlap); and other cysteine synthase proteins e.g. Rv1336, Rv0848, etc. Contains PS00217 Sugar transport proteins signature 2 probably spurious. Belongs to the cysteine synthase/cystathionine beta-synthase family.; Probable cystathionine beta-synthase Cbs (serine sulfhydrase) (beta-thionase) (hemoprotein H-450) 1203111 cbs 887108 cbs Mycobacterium tuberculosis H37Rv Probable cystathionine beta-synthase Cbs (serine sulfhydrase) (beta-thionase) (hemoprotein H-450) YP_177782.1 1201717 D 83332 CDS NP_215594.1 15608218 887111 1203313..1204035 1 NC_000962.3 Rv1078, (MTV017.31), len: 240 aa. Probable pra,Proline-rich antigen homolog, equivalent to X65546|MLPRAG_1 proline rich antigen from Mycobacterium leprae (249 aa),FASTA scores: opt: 1162, E(): 3.3e-30, (64.8% identity in 253 aa overlap). Has potential hydrophobic domains.; Probable proline-rich antigen homolog Pra 1204035 pra 887111 pra Mycobacterium tuberculosis H37Rv Probable proline-rich antigen homolog Pra NP_215594.1 1203313 D 83332 CDS NP_215595.1 15608219 887103 1204067..1205233 1 NC_000962.3 Rv1079, (MTV017.32), len: 388 aa. metB,cystathionine gamma-synthase (see citation below). P46807|METB_MYCLE cystathionine gamma-synthase from Mycobacterium leprae (388 aa), FASTA scores: opt: 2220,E(): 0, (87.3% identity in 387 aa overlap). Also similar to other Mycobacterium tuberculosis enzymes involved in methionine synthesis e.g. Rv0391 and Rv3340. Contains PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. Belongs to the trans-sulfuration enzymes family.; Cystathionine gamma-synthase MetB (CGS) (O-succinylhomoserine [thiol]-lyase) 1205233 metB 887103 metB Mycobacterium tuberculosis H37Rv Cystathionine gamma-synthase MetB (CGS) (O-succinylhomoserine [thiol]-lyase) NP_215595.1 1204067 D 83332 CDS NP_215596.1 15608220 887115 complement(1205304..1205798) 1 NC_000962.3 Rv1080c, (MTV017.33c), len: 164 aa. Probable greA,transcription elongation factor G, closest to P46808|GREA_MYCLE transcription elongation factor G from Mycobacterium leprae (202 aa), FASTA scores: opt: 1005,E(): 0, (94.5% identity in 164 aa overlap); and similar to many e.g. P21346|GREA_ECOLI from Escherichia coli (158 aa),FASTA scores: opt: 257, E(): 5.7e-10, (37.2% identity in 148 aa overlap); etc. Contains two PS00829 and one PS00830 Prokaryotic transcription elongation factors signatures 1 and 2, respectively. Belongs to the GREA/GREB family.; Probable transcription elongation factor GreA (transcript cleavage factor GreA) 1205798 greA 887115 greA Mycobacterium tuberculosis H37Rv Probable transcription elongation factor GreA (transcript cleavage factor GreA) NP_215596.1 1205304 R 83332 CDS NP_215597.1 15608221 887102 complement(1205984..1206418) 1 NC_000962.3 Rv1081c, (MTV017.34c), len: 144 aa. Probable conserved membrane protein, with hydrophobic stretch from aa 26 - 48, highly similar to NP_302548.1|NC_002677 conserved membrane protein from Mycobacterium leprae. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein 1206418 887102 Rv1081c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_215597.1 1205984 R 83332 CDS NP_215598.1 15608222 887101 1206520..1207386 1 NC_000962.3 Rv1082, (MTV017.35), len: 288 aa. Mca, mycothiol conjugate amidase (see citation below), equivalent to NP_302547.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (290 aa), FASTA scores: opt: 1737,E(): 0, (86.4% identity in 287 aa overlap); and similar to Q54358|X79146 lmbE protein from Streptomyces lincolnensis (270 aa). Also similar to Rv1170|MTV005.06|MSHB GlcNAc-Ins deacetylase from Mycobacterium tuberculosis (303 aa), FASTA scores: opt: 411, E(): 9.4e-20, (35.8% identity in 299 aa overlap).; Mycothiol conjugate amidase Mca (mycothiol S-conjugate amidase) 1207386 mca 887101 mca Mycobacterium tuberculosis H37Rv Mycothiol conjugate amidase Mca (mycothiol S-conjugate amidase) NP_215598.1 1206520 D 83332 CDS NP_215599.1 15608223 887100 1207383..1207649 1 NC_000962.3 Rv1083, (MTV017.36), len: 88 aa. Conserved hypothetical protein, similar to U15183|MLU15183_9 hypothetical protein from Mycobacterium leprae (167 aa),FASTA scores: opt: 332, E(): 1.2e-13, (58.4% identity in 101 aa overlap). Hydrophobic domain aa 25-43. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1207649 887100 Rv1083 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215599.1 1207383 D 83332 CDS NP_215600.1 15608224 887098 1207636..1209657 1 NC_000962.3 Rv1084, (MTV017.37), len: 673 aa. Conserved protein,similar to P37512|YYAL_BACSU hypothetical protein from Bacillus subtilis (689 aa), FASTA scores: opt: 1063, E() : 0, (36.5% identity in 696 aa overlap); AE0009|AE000983_10 Archaeoglobus fulgidus section 1 (642 aa), FASTA scores: opt: 1018, E(): 0, (37.2% identity in 600 aa overlap). Also similar to AE001938|AE001938_9 Deinococcus radiodurans (690 aa), FASTA scores: opt: 1097, E(): 0, (41.6% identity in 694 aa overlap).; hypothetical protein 1209657 887098 Rv1084 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215600.1 1207636 D 83332 CDS NP_215601.1 15608225 887107 complement(1209756..1210484) 1 NC_000962.3 Rv1085c, (MTV017.38c), len: 242 aa. Possible hemolysin-like protein, integral membrane protein, similar to many hemolysins, and hypothetical proteins e.g. U28375|ECU28375_49 Hypothetical protein from Escherichia coli (219 aa), FASTA scores: opt: 308, E(): 7.5e-15, (30.6% identity in 180 aa overlap); AE0011|HIAE001124_2 Hypothetical protein from Borrelia burgdorferi (233 aa),FASTA scores: opt: 305, E(): 1.3e-14, (25.6% identity in 203 aa overlap). Also weakly similar to HLY3_BACCE|P54176 haemolysin from Bacillus cereus (219 aa), FASTA scores: opt: 247, E(): 8.7e-12, (27.5% identity in 171 aa overlap). Also similar to AE002027|AE002027_8 probable hemolysin from Deinococcus radiodurans (219 aa), FASTA scores: opt: 354,E(): 1.8e-16, (31.1% identity in 219 aa overlap).; Possible hemolysin-like protein 1210484 887107 Rv1085c Mycobacterium tuberculosis H37Rv Possible hemolysin-like protein NP_215601.1 1209756 R 83332 CDS NP_215602.1 15608226 887097 1210595..1211383 1 NC_000962.3 Rv1086, (MTV017.39), len: 262 aa. Short (C15) chain Z-isoprenyl diphosphate synthase (see citations below),equivalent to NP_302598.1|NC_002677 possible undecaprenyl pyrophosphate synthetase from Mycobacterium leprae (262 aa), similar to many hypothetical proteins and several potential members of the upp synthase family e.g. NP_296167.1|NC_001263 undecaprenyl diphosphate synthase from Deinococcus radiodurans (339 aa); P20182|YT14_STRFR Hypothetical protein from Streptomyces fradiae (259 aa),FASTA scores: opt: 840, E(): 0, (51.0% identity in 259 aa overlap); and P38118|YARF_CORGL Hypothetical protein from Corynebacterium glutamicicum (234 aa), FASTA scores: opt: 729, E(): 0, (56.0% identity in 209 aa overlap); etc. Also similar to Rv2361c|MTCY27.19 (296 aa) (35.6% identity in 233 aa overlap). Contains PS01066 Uncharacterized protein family UPF0015 signature. Seems to belong to the UPP synthetase family.; Short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase) 1211383 887097 Rv1086 Mycobacterium tuberculosis H37Rv Short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase) NP_215602.1 1210595 D 83332 CDS YP_177783.1 57116821 887094 1211560..1213863 1 NC_000962.3 Rv1087, (MTV017.40), len: 767 aa. PE_PGRS21, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to Rv1090|AL021897|MTV017_43 Mycobacterium tuberculosis H37Rv (853 aa), FASTA scores: opt: 2819, E(): 0, (59.8% identity in 860 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1 near C -terminus. Predicted to be an outer membrane protein (See Song et al.,2008).; PE-PGRS family protein PE_PGRS21 1213863 PE_PGRS21 887094 PE_PGRS21 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS21 YP_177783.1 1211560 D 83332 CDS YP_177637.1 57116822 3205067 1214040..1214360 1 NC_000962.3 Rv1087A, len: 106 aa (fragment). Conserved hypothetical protein, highly similar to C-terminus of near ORF O53434|YA86_MYCTU|Rv1086|MT1118|MTV017.39 short (C15) chain Z-isoprenyl diphosphate synthase from Mycobacterium tuberculosis (262 aa), FASTA scores: opt: 200, E(): 1.1e-06, (57.9% identity in 76 aa overlap).; hypothetical protein 1214360 3205067 Rv1087A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177637.1 1214040 D 83332 CDS YP_177784.1 57116823 887096 1214513..1214947 1 NC_000962.3 Rv1088, (MTV017.41), len: 144 aa. PE9, Member of Mycobacterium tuberculosis PE family (see citation below),similar to many others e.g. Z96071|MTCI418B_6 Mycobacterium tuberculosis cosmid (487 aa), FASTA scores: opt: 318, E(): 7.3e-14, (60.9% identity in 87 aa overlap) - except it appears to be frameshifted around codon 84. No error to account for frameshift could be found.; PE family protein PE9 1214947 PE9 887096 PE9 Mycobacterium tuberculosis H37Rv PE family protein PE9 YP_177784.1 1214513 D 83332 CDS YP_177785.1 57116824 887090 <1214769..1215131 1 NC_000962.3 Rv1089, (MTV017.42), len: 120 aa. PE10, Member of the Mycobacterium tuberculosis PE family of glycine-rich proteins (see citation below). Partial ORF that appears to be frameshifted continuation of Rv1088|MTV017.41. Sequence has been checked and appears correct. Similar to Z95555|MTCY06F7_4 Mycobacterium tuberculosis cosmid (401 aa), FASTA scores: opt:126, E(): 2, (29.6% identity in 125 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE family protein PE10 1215131 PE10 887090 PE10 Mycobacterium tuberculosis H37Rv PE family protein PE10 YP_177785.1 <1214769 D 83332 CDS YP_177638.1 57116825 3205066 1215517..1215621 1 NC_000962.3 Rv1089A, len: 34 aa. Probable celA2a, first part of cellulase (endoglucanase), similar to N-terminus of others. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable cellulase CelA2a (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) 1215621 celA2a 3205066 celA2a Mycobacterium tuberculosis H37Rv Probable cellulase CelA2a (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) YP_177638.1 1215517 D 83332 CDS NP_215606.1 15608230 885637 1215599..1216054 1 NC_000962.3 Rv1090, (MTV017.43), len: 151 aa. Probable celA2b,second part of cellulase (endoglucanase), similar to C-terminus of others e.g. O08468 cellulase CEL2 from Streptomyces halstedi (377 aa), FASTA scores: opt: 554,E(): 1.2e-30, (52.0% identity in 152 aa overlap); etc. Gene appears to have been inactivated by frameshift mutations but no errors could be found that would account for this. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable cellulase CelA2b (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) 1216054 celA2b 885637 celA2b Mycobacterium tuberculosis H37Rv Probable cellulase CelA2b (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) NP_215606.1 1215599 D 83332 CDS YP_177786.1 57116826 885258 1216469..1219030 1 NC_000962.3 Rv1091, (MTV017.44), len: 853 aa. PE_PGRS22, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to Rv1087|AL021897|MTV017_39 Mycobacterium tuberculosis H37Rv (767 aa), FASTA scores: opt: 2819, E(): 0, (60.0% identity in 860 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; PE-PGRS family protein PE_PGRS22 1219030 PE_PGRS22 885258 PE_PGRS22 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS22 YP_177786.1 1216469 D 83332 CDS NP_215608.1 15608232 885120 complement(1219248..1220186) 1 NC_000962.3 Rv1092c, (MTV017.45c), len: 312 aa. Probable coaA,pantothenate kinase, similar to many e.g. P15044|COAA_ECOLI Escherichia coli (316 aa), FASTA scores :opt: 1079, E(): 0,(52.7% identity in 311 aa overlap). Equivalent to AL049491|MLCB1222_17 Mycobacterium leprae (312 aa) (93.6% identity in 312 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the pantothenate kinase family.; Probable pantothenate kinase CoaA (pantothenic acid kinase) 1220186 coaA 885120 coaA Mycobacterium tuberculosis H37Rv Probable pantothenate kinase CoaA (pantothenic acid kinase) NP_215608.1 1219248 R 83332 CDS YP_177787.3 448824753 885338 1220574..1221854 1 NC_000962.3 Rv1093, (MTV017.46), len: 426 aa. glyA1, serine hydroxymethyltransferase 1, equivalent to AL049491|MLCB1222_16 from Mycobacterium leprae (426 aa),FASTA score: (89.9 % identity in 426 aa overlap). Also similar to many e.g. P34895|GLYA_HYPME hyphomicrobium methylovorum (434 aa), FASTA scores: opt: 1492, E(): 0,(56.8% identity in 419 aa overlap); etc. Belongs to the ShmT family. Note that previously known as glyA.; Serine hydroxymethyltransferase 1 GlyA1 1221854 glyA1 885338 glyA1 Mycobacterium tuberculosis H37Rv Serine hydroxymethyltransferase 1 GlyA1 YP_177787.3 1220574 D 83332 CDS NP_215610.1 15608234 885339 1221959..1222786 1 NC_000962.3 Rv1094, (MTV017.47), len: 275 aa. Possible desA2,acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase), equivalent to AL049491|MLCB1222_15 from Mycobacterium leprae (275 aa), FASTA score: (78.1% identity in 274 aa overlap). Also weakly similar to plant stearoyl-acyl carrier protein desaturases, and very similar to U49839|MTV043.16C|Rv0824c enzyme desA1 from Mycobacterium tuberculosis (338 aa), FASTA scores: opt: 525, E(): 8.5e-30, (32.2% identity in 270 aa overlap); and to U15182|MLU15182_32 acyl-carrier protein desaturase precursor from Mycobacterium leprae (338 aa), FASTA scores: opt: 506, E(): 1.9e-28, (34.1% identity in 261 aa overlap).; Possible acyl-[acyl-carrier protein] desaturase DesA2 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) 1222786 desA2 885339 desA2 Mycobacterium tuberculosis H37Rv Possible acyl-[acyl-carrier protein] desaturase DesA2 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) NP_215610.1 1221959 D 83332 CDS NP_215611.1 15608235 885592 1222997..1224298 1 NC_000962.3 Rv1095, (MTV017.48), len: 433 aa. Probable phoH2,phoH-like protein (phosphate starvation-induced protein),probably ATP-binding protein. Equivalent to AL049491 MLCB1222_14 Mycobacterium leprae (433 aa) (92.8% identity in 432 aa overlap). Similar to many proteins described as PhoH-like e.g. Z97025|BSZ97025_12 Bacillus subtilis (442 aa), FASTA scores: opt: 605, E(): 0, (40.1% identity in 444 aa overlap); or Mycobacterium tuberculosis Rv2368c|O05830|PHOL_MYCTU Mycobacterium tuberculosis (352 aa), FASTA scores: opt: 390, E(): 4e-19, (31.5% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the PhoH family.; Probable PHOH-like protein PhoH2 (phosphate starvation-inducible protein PSIH) 1224298 phoH2 885592 phoH2 Mycobacterium tuberculosis H37Rv Probable PHOH-like protein PhoH2 (phosphate starvation-inducible protein PSIH) NP_215611.1 1222997 D 83332 CDS NP_215612.1 15608236 885949 1224385..1225260 1 NC_000962.3 Rv1096, (MTV017.49), len: 291 aa. Possible glycosyl hydrolase, possibly deacetylase or esterase. Equivalent to AL049491|MLCB1222_13 Mycobacterium leprae (291 aa) (81.3% identity in 289 aa overlap). Similar at the C-terminus to enzymes involved in carbohydrate degradation including Z99110|BSUB0007_92 endo-1,4-beta-xylanase homolog yjeA from Bacillus subtilis (467 aa), FASTA scores: opt: 418, E(): 2.6e-17, (38.6% identity in 184 aa overlap); M64552|STMXLNB_2 acetyl-xylan esterase from Streptomyces lividans (335 aa), FASTA scores: opt: 371, E(): 1.1e-14,(31.6% identity in 237 aa overlap); NP_345933.1|NC_003028 peptidoglycan N-acetylglucosamine deacetylase a from Streptococcus pneumoniae (463 aa); etc. Has possible N-terminal signal sequence with TMhelix at aa 13-31.; Possible glycosyl hydrolase 1225260 885949 Rv1096 Mycobacterium tuberculosis H37Rv Possible glycosyl hydrolase NP_215612.1 1224385 D 83332 CDS NP_215613.1 15608237 885962 complement(1225263..1226144) 1 NC_000962.3 Rv1097c, (MTV017.50c), len: 293 aa. Probable membrane Gly-, Pro-rich protein, similar to Mycobacterium tuberculosis Rv2507|MTCY07A7. 13|Z95556 (273 aa), FASTA scores: opt: 219, E(): 0.023, (30.5% identity in 266 aa overlap); and Rv2507. Contains potential membrane spanning region (aa ~68-92).; Probable membrane glycine and proline rich protein 1226144 885962 Rv1097c Mycobacterium tuberculosis H37Rv Probable membrane glycine and proline rich protein NP_215613.1 1225263 R 83332 CDS NP_215614.1 15608238 885651 complement(1226141..1227565) 1 NC_000962.3 Rv1098c, (MTV017.51c), len: 474 aa. Probable fum,fumarase. Equivalent to AL049491|MLCB1222_11 Mycobacterium leprae (474 aa) (89.5 % identity in 467 aa overlap). Similar to many e.g. P14408|FUMH_RAT fumarate hydratase,mitochondrial precursor from Rattus norvegicus (507 aa),FASTA scores: opt: 1427, E(): 0, (52.3% identity in 461 aa overlap); and P05042|FUMC_ECOLI Fumarate hydratase class II from Escherichia coli (467 aa), FASTA scores: opt: 1355,E(): 0, (50.2% identity in 444 aa overlap). Contains PS00163 Fumarate lyases signature.; Probable fumarase Fum (fumarate hydratase) 1227565 fum 885651 fum Mycobacterium tuberculosis H37Rv Probable fumarase Fum (fumarate hydratase) NP_215614.1 1226141 R 83332 CDS NP_215615.3 448824754 885861 complement(1227596..1228684) 1 NC_000962.3 Rv1099c, (MTV017.52c), len: 362 aa. glpX, class II fructose 1,6-bisphosphatase (See Movahedzadeh et al.,2004), highly similar to P44811|GLPX_HAEIN GLPX protein homolog (believed to be involved in glycerol metabolism) (333 aa), FASTA scores: opt: 763, E():0, (46.2% identity in 327 aa overlap); and Q03224|YWJI_BACSU hypothetical protein from Bacillus subtilis (321aa), FASTA scores: opt: 1092,E(): 0, (52.1% identity in 313 aa overlap). Equivalent to AL049491|MLCB1222_10 Mycobacterium leprae (355 aa), (93.0% identity in 328 aa overlap). N-terminus extended since first submission (previously 328 aa).; Fructose 1,6-bisphosphatase GlpX 1228684 glpX 885861 glpX Mycobacterium tuberculosis H37Rv Fructose 1,6-bisphosphatase GlpX NP_215615.3 1227596 R 83332 CDS NP_215616.1 15608240 885852 1228683..1229384 1 NC_000962.3 Rv1100, (MTV017.53), len: 233 aa. Conserved protein,slightly similar to Rv1906c|MTCY180.12 hypothetical protein from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 122, E(): 6.9, (27.4% identity in 135 aa overlap). Equivalent to AL049491|MLCB1222_9 Mycobacterium leprae (257 aa) (63.8% identity in 257 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1229384 885852 Rv1100 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215616.1 1228683 D 83332 CDS NP_215617.1 15608241 885482 complement(1229391..1230548) 1 NC_000962.3 Rv1101c, (MTV017.54c), len: 385 aa. Conserved membrane protein, shows some similarity to other bacterial proteins e.g. P77406|PERM_ECOLI putative permease perm from Escherichia coli (353 aa), FASTA scores: opt: 287, E(): 8.8e-12, (24.9% identity in 349 aa overlap).; hypothetical protein 1230548 885482 Rv1101c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215617.1 1229391 R 83332 CDS NP_215618.1 15608242 886001 complement(1230660..1230971) 1 NC_000962.3 Rv1102c, (MTV017.55c), len: 103 aa. MazF3, toxin,part of toxin-antitoxin (TA) operon with Rv1103c (See Pandey and Gerdes, 2005; Zhu et al., 2006), similar to Mycobacterium tuberculosis hypothetical protens e.g. Rv1942c|MTCY9F9_22 (109 aa), FASTA scores: opt: 158, E(): 3.6e-05, (33.3% identity in 93 aa overlap); Rv0659c|MTCI376_17 (102aa), opt: 140, E(): 0.00072, (40.6% identity in 69aa overlap); and Rv1495.; Toxin MazF3 1230971 mazF3 886001 mazF3 Mycobacterium tuberculosis H37Rv Toxin MazF3 NP_215618.1 1230660 R 83332 CDS NP_215619.1 15608243 885941 complement(1230971..1231291) 1 NC_000962.3 Rv1103c, (MTV017.56c), len: 106 aa. Possible mazE3,antitoxin, part of toxin-antitoxin (TA) operon with Rv1102c (See Pandey and Gerdes, 2005; Zhu et al., 2006). Note that Zhu et al., 2006 identifies a different amino acid sequence as the possible antitoxin to Rv1102c. Similar to part of Mycobacterium tuberculosis hypothetical protein Rv2472|AL021246|MTV008_27 Mycobacterium tuberculosis (97 aa), FASTA score: opt: 135, E(): 0.0091, (45.8% identity in 72 aa overlap).; Possible antitoxin MazE3 1231291 mazE3 885941 mazE3 Mycobacterium tuberculosis H37Rv Possible antitoxin MazE3 NP_215619.1 1230971 R 83332 CDS NP_215620.1 15608244 885835 1231301..1231990 1 NC_000962.3 Rv1104, (MTV017.57), len: 229 aa. Possible para-nitrobenzyl esterase (fragment; possibly first part) . Similar to the N-terminal domain of many e.g. P37967|PNBA_BACSU Bacillus subtilis (489 aa), FASTA scores: opt: 715, E(): 0, (53.4% identity in 191 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing in MTV017.58|Rv1105.; Possible para-nitrobenzyl esterase (fragment) 1231990 885835 Rv1104 Mycobacterium tuberculosis H37Rv Possible para-nitrobenzyl esterase (fragment) NP_215620.1 1231301 D 83332 CDS NP_215621.2 57116828 885977 1232311..1232826 1 NC_000962.3 Rv1105, (MTV017.58), len: 171 aa. Possible para-nitrobenzyl esterase (fragment; possibly second part) . Similar to C-terminal domain of many e.g. P71048 para-nitrobenzyl esterase from Bacillus subtilis (489 aa),FASTA scores: opt: 248, E(): 2.7e-10, (32.3% identity in 167 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing from MTV017.57|Rv1104. Start changed since first submission.; Possible para-nitrobenzyl esterase (fragment) 1232826 885977 Rv1105 Mycobacterium tuberculosis H37Rv Possible para-nitrobenzyl esterase (fragment) NP_215621.2 1232311 D 83332 CDS NP_215622.1 15608246 886004 complement(1232844..1233956) 1 NC_000962.3 Rv1106c, (MTV017.59c), len: 370 aa. 3-beta-hydroxysteroid dehydrogenase (see Yang et al.,2007). Equivalent to AL049491|MLCB1222_7 Mycobacterium leprae (376 aa) (75.5% identity in 375 aa overlap). Highly similar to Q03704 NAD(P)-dependent cholesterol dehydrogenase from Nocardia sp. (364 aa), FASTA scores: opt: 1789, E(): 0, (74.5% identity in 361 aa overlap). Also similar to U32426|MCU32426_1 3-beta-hydroxy-Delta5-steroid dehydrogenase from Molluscum contagiosum virus (354 aa),FASTA scores: opt: 432, E(): 1.7e-22, (34.6% identity in 347 aa overlap). Also similar to series of Mycobacterium tuberculosis hypothetical proteins described as sugar epimerases or dehydratases e.g. Rv3634c, Rv3784, Rv3464,etc. The transcription of this CDS seems to be activated specifically in host granulomas (see Ramakrishnan et al.,2000).; 3-beta-hydroxysteroid dehydrogenase 1233956 886004 Rv1106c Mycobacterium tuberculosis H37Rv 3-beta-hydroxysteroid dehydrogenase NP_215622.1 1232844 R 83332 CDS NP_215623.1 15608247 886005 complement(1233966..1234223) 1 NC_000962.3 Rv1107c, (MTV017.60c), len: 85 aa. Probable xseB,exonuclease VII small subunit (see citation below). Equivalent to AL049491|MLCB1222_6 Mycobacterium leprae (87 aa) (77.9% identity in 68 aa overlap). Similar to P43914|EX7S_HAEIN exodeoxyribonuclease small subunit from H. influenzae (84 aa), FASTA scores: opt: 126, E(): 0.006,(37.3% identity in 67 aa overlap); and P22938|EX7S_ECOLI exodeoxyribonuclease small subunit from Escherichia coli (79 aa), FASTA scores: opt: 125, E(): 0.0067, (39.7% identity in 58 aa overlap). Belongs to the XseB family.; Probable exodeoxyribonuclease VII (small subunit) XseB (exonuclease VII small subunit) 1234223 xseB 886005 xseB Mycobacterium tuberculosis H37Rv Probable exodeoxyribonuclease VII (small subunit) XseB (exonuclease VII small subunit) NP_215623.1 1233966 R 83332 CDS NP_215624.1 15608248 885984 complement(1234213..1235460) 1 NC_000962.3 Rv1108c, (MTV017.61c), len: 415 aa. Probable xseA,exodeoxyribonuclease VII large subunit (see Mizrahi & Andersen 1998). Equivalent to AL049491|MLCB1222_5 Mycobacterium leprae (428 aa) (81.5% identity in 411 aa overlap). Similar to many e.g. P04994|EX7L_ECOLI exodeoxyribonuclease large subunit from Escherichia coli (456 aa), FASTA scores: opt: 581, E(): 1.6 e-30, (30.8% identity in 425 aa overlap); also similar to the exodeoxyribonuclease in Bacillus subtilis, H. influenzae and H. pylori. Belongs to the XseA family.; Probable exodeoxyribonuclease VII (large subunit) XseA (exonuclease VII large subunit) 1235460 xseA 885984 xseA Mycobacterium tuberculosis H37Rv Probable exodeoxyribonuclease VII (large subunit) XseA (exonuclease VII large subunit) NP_215624.1 1234213 R 83332 CDS NP_215625.1 15608249 885828 complement(1235457..1236095) 1 NC_000962.3 Rv1109c, (MTV017.62c), len: 212 aa. Conserved protein. Equivalent to AL049491|MLCB1222_4 hypothetical protein from Mycobacterium leprae (205 aa) (68.1% identity in 213 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1236095 885828 Rv1109c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215625.1 1235457 R 83332 CDS YP_177788.1 57116829 885830 1236185..1237192 1 NC_000962.3 Rv1110, (MTV017.63), len: 335 aa. Probable lytB2,LytB-related protein, equivalent to AL049491|MLCB1222_3 from Mycobacterium leprae (335 aa), FASTA score: (82.9% identity in 333 aa overlap). Also similar to LytB proteins from many bacteria (appears to have N-terminal extension) e.g. P22565|LYTB_ECOLI|B0029|Z0034|ECS0032 LYTB protein from Escherichia coli strains K12 and O157:H7 (316 aa),FASTA scores: opt: 1041, E():0, (52.4% identity in 309 aa overlap); etc. Also very similar to another LytB-related protein from Mycobacterium tuberculosis: LytB1|Rv3382c|MTV004.40c (329 aa), FASTA scores: opt: 975,E(): 0, (51.3% identity in 312 aa overlap). Belongs to the LytB family.; Probable LYTB-related protein LytB2 1237192 lytB2 885830 lytB2 Mycobacterium tuberculosis H37Rv Probable LYTB-related protein LytB2 YP_177788.1 1236185 D 83332 CDS NP_215627.2 57116830 885447 complement(1237209..1238192) 1 NC_000962.3 Rv1111c, (MTV017.64c), len: 327 aa. Conserved hypothetical protein, N-terminal domain is hydrophobic,C-terminal half is very rich in Arg. Equivalent to AL049491|MLCB1222_2 hypothetical protein from Mycobacterium leprae (379 aa) (46.0% identity in 374 aa overlap). Start changed since first submission. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 1238192 885447 Rv1111c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215627.2 1237209 R 83332 CDS NP_215628.1 15608252 887151 1238255..1239328 1 NC_000962.3 Rv1112, (MTCY22G8.01-MTV017.65), len: 357 aa. Probable GTP binding protein, similar to YCHF_HAEIN|P44681 probable gtp-binding protein (362 aa), FASTA scores: opt: 1189, E(): 0, (52.7% identity in 357 aa overlap). Equivalent to AL049491|MLCB1222_1 hypothetical protein from Mycobacterium leprae (356 aa) (85.9% identity in 354 aa overlap0. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable GTP binding protein 1239328 887151 Rv1112 Mycobacterium tuberculosis H37Rv Probable GTP binding protein NP_215628.1 1238255 D 83332 CDS NP_215629.1 15608253 885450 1239416..1239613 1 NC_000962.3 Rv1113, (MTCY22G8.02), len: 65 aa. Possible vapB32,antitoxin, part of toxin-antitoxin (TA) operon with Rv1114,see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2758c|AL00896 7|MTV002.23 (88 aa) FASTA scores: opt: 97, E(): 0.86, (33.3% identity in 69 aa overlap). Part of family including Rv2871, Rv1241, Rv2132,Rv3321c, etc.; Possible antitoxin VapB32 1239613 vapB32 885450 vapB32 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB32 NP_215629.1 1239416 D 83332 CDS NP_215630.1 15608254 885490 1239610..1239984 1 NC_000962.3 Rv1114, (MTCY22G8.03), len: 124 aa. Possible vapC32,toxin, part of toxin-antitoxin (TA) operon with Rv1113,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv1561 and Rv2010.; Possible toxin VapC32 Contains PIN domain 1239984 vapC32 885490 vapC32 Mycobacterium tuberculosis H37Rv Possible toxin VapC32 Contains PIN domain NP_215630.1 1239610 D 83332 CDS NP_215631.1 15608255 885951 1240187..1240885 1 NC_000962.3 Rv1115, (MTCY22G8.04), len: 232 aa. Possible exported protein, contains possible N-terminal signal sequence.; Possible exported protein 1240885 885951 Rv1115 Mycobacterium tuberculosis H37Rv Possible exported protein NP_215631.1 1240187 D 83332 CDS NP_215632.1 15608256 885969 1241003..1241188 1 NC_000962.3 Rv1116, (MTCY22G8.05), len: 61 aa. Hypothetical unknown protein.; Hypothetical protein 1241188 885969 Rv1116 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215632.1 1241003 D 83332 CDS YP_177639.1 57116831 3205101 complement(1241115..1241390) 1 NC_000962.3 Rv1116A, len: 91 aa. Conserved hypothetical protein (possibly gene fragment), similar to C-terminal part of Rv1646|Z85982_9 from Mycobacterium tuberculosis (310 aa),FASTA scores: opt: 301, E(): 9.3e-13, (68.05% identity in 72 aa overlap). Also overlaps gene on other strand, Rv1116,at 3'-end.; Conserved hypothetical protein (fragment) 1241390 3205101 Rv1116A Mycobacterium tuberculosis H37Rv Conserved hypothetical protein (fragment) YP_177639.1 1241115 R 83332 CDS NP_215633.1 15608257 885847 1241633..1241956 1 NC_000962.3 Rv1117, (MTCY22G8.06), len: 107 aa. Conserved protein, some similarity to P94425|D50453 hypothetical protein from Bacillus subtilis (95 aa), fasta scores: opt: 128, E(): 5.1e-06, (28.3% identity in 92 aa overlap); and AL117322|SCF1.02 Streptomyces coelicolor (109 aa), FASTA scores: opt: 437, E(): 1.6e-25, (57.5% identity in 106 aa overlap).; hypothetical protein 1241956 885847 Rv1117 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215633.1 1241633 D 83332 CDS NP_215634.1 15608258 888936 complement(1241971..1242831) 1 NC_000962.3 Rv1118c, (MTCY22G8.07c), len: 286 aa. Conserved protein, similar to pseudogene ML0942 in Mycobacterium leprae.; hypothetical protein 1242831 888936 Rv1118c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215634.1 1241971 R 83332 CDS NP_215635.1 15608259 885853 complement(1242864..1243013) 1 NC_000962.3 Rv1119c, (MTCY22G8.08c), len: 49 aa. Hypothetical unknown protein.; Hypothetical protein 1243013 885853 Rv1119c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215635.1 1242864 R 83332 CDS NP_215636.1 15608260 885633 complement(1243010..1243504) 1 NC_000962.3 Rv1120c, (MTCY22G8.09c), len: 164 aa. Conserved hypothetical protein, some similarity at C-terminus to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1890c|MTCY180.28 (462 aa), FASTA scores: opt: 187, E(): 2.2e-05, (36.6% identity in 93 aa overlap) and Rv2488c|YZ19_MYCTU|Q10551 (285 aa), FASTA scores: opt: 156,E(): 0.00074, (32.7% identity in 107 aa overlap).; hypothetical protein 1243504 885633 Rv1120c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215636.1 1243010 R 83332 CDS YP_177789.1 57116832 885817 1243707..1245107 1 NC_000962.3 Rv1121, (MTCY22G8.10), len: 466 aa. Probable zwf1,glucose-6-phosphate 1-dehydrogenase, highly similar to many e.g. G6PD_E COLI|P22992 Escherichia coli (491 aa), FASTA scores: opt: 642, E(): 0, (35.8% identity in 478 aa overlap). Mycobacterium tuberculosis has two genes for ZWF,this one is highly divergent. Belongs to the glucose-6-phosphate dehydrogenase family. Note that previously known as zwf. Nucleotide position 1244700 in the genome sequence has been corrected, T:C resulting in L332L.; Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD) 1245107 zwf1 885817 zwf1 Mycobacterium tuberculosis H37Rv Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD) YP_177789.1 1243707 D 83332 CDS NP_215638.1 15608262 885820 1245129..1246151 1 NC_000962.3 Rv1122, (MTCY22G8.11), len: 340 aa. Probable gnd2,6-phosphogluconate dehydrogenase, decarboxylating, highly similar to Q53917 6-phosphogluconate dehydrogenase from Streptomyces coelicolor (291 aa), fasta scores: opt: 431,E(): 2.2e-20, (44.5% identity in 335 aa overlap). Also similar to Rv1844c|MTCY359.29|gnd1 probable 6-phosphogluconate dehydrogenase from Mycobacterium tuberculosis (485 aa), FASTA score: (33.0% identity in 351 aa overlap). Note that Rv1844c|MTCY359.29|gnd1 is most similar to gnd's from Gram negative organisms, while gnd2 is most similar to gnd's from Gram positive organisms. Belongs to the 6-phosphogluconate dehydrogenase family.; Probable 6-phosphogluconate dehydrogenase,decarboxylating Gnd2 1246151 gnd2 885820 gnd2 Mycobacterium tuberculosis H37Rv Probable 6-phosphogluconate dehydrogenase,decarboxylating Gnd2 NP_215638.1 1245129 D 83332 CDS NP_215639.1 15608263 885965 complement(1246144..1247052) 1 NC_000962.3 Rv1123c, (MTCY22G8.12c), len: 302 aa. Possible bpoB,peroxidase (non-haem peroxidase), with some similarity to a range of enzymes from several organisms including: DEH1_MORSP|Q01398 haloacetate dehalogenase from Moraxella sp. (294 aa), FASTA scores: opt: 201, E(): 2.1e-06, (35.8% identity in 134 aa overlap); and BPA1_STRAU|P33912 non-haem bromoperoxidase bpo-a1 from Streptomyces aureofaciens (274 aa), FASTA scores: opt: 187, E(): 1.6e-05, (23.1% identity in 281 aa overlap). Similar to several other Mycobacterium tuberculosis proteins, probable epoxide hydrolases and non-heme bromoperoxidases e.g. Rv1938, Rv3617, Rv3473c,Rv3171c, etc. Contains PS00216 Sugar transport proteins signature 1.; Possible peroxidase BpoB (non-haem peroxidase) 1247052 bpoB 885965 bpoB Mycobacterium tuberculosis H37Rv Possible peroxidase BpoB (non-haem peroxidase) NP_215639.1 1246144 R 83332 CDS NP_215640.1 15608264 886022 1247127..1248077 1 NC_000962.3 Rv1124, (MTCY22G8.13), len: 316 aa. Probable ephC,epoxide hydrolase (see citation below), similar to Q42566 epoxide hydrolase from Arabidopsis thaliana (321 aa), FASTA scores: opt: 298, E(): 8.2e-13, (27.6% identity in 333 aa overlap). Similar to other M. tuberculosis epoxide hydrolases and non-heme bromoperoxidases e.g. Rv1938,Rv3617, Rv3670, Rv3473c, etc.; Probable epoxide hydrolase EphC (epoxide hydratase) 1248077 ephC 886022 ephC Mycobacterium tuberculosis H37Rv Probable epoxide hydrolase EphC (epoxide hydratase) NP_215640.1 1247127 D 83332 CDS NP_215641.1 15608265 886021 1248082..1249326 1 NC_000962.3 Rv1125, (MTCY22G8.14), len: 414 aa. Conserved hypothetical protein. Similar to AL133278|SCM11.13 hypothetical protein from Streptomyces coelicolor (446 aa),FASTA scores: opt: 182, E(): 0.0005, (28.1% identity in 437 aa overlap).; hypothetical protein 1249326 886021 Rv1125 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215641.1 1248082 D 83332 CDS NP_215642.1 15608266 885845 complement(1249330..1249935) 1 NC_000962.3 Rv1126c, (MTCY22G8.15c), len: 201 aa. Conserved protein, similar in N-terminus to O05567|MLCB33.17 hypothetical protein from Mycobacterium leprae (141 aa),FASTA scores: opt: 332, E(): 1.4e-23, (58.4% identity in 101 aa overlap).; hypothetical protein 1249935 885845 Rv1126c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215642.1 1249330 R 83332 CDS NP_215643.1 15608267 885956 complement(1249932..1251404) 1 NC_000962.3 Rv1127c, (MTCY22G8.16c), len: 490 aa. Probable ppdK,Pyruvate, phosphate dikinase. Equivalent (but shorter) to Z94723|MLCB33_16 ppdK from Mycobacterium leprae (601 aa) (71.8% identity in 478 aa overlap). Highly similar to N-terminus of PODK_CLOSY|P22983 pyruvate, phosphate dikinase from Clostridium symbiosum (873 aa), FASTA scores: opt: 786, E(): 0, (37.4% identity in 514 aa overlap).; Probable pyruvate, phosphate dikinase PpdK 1251404 ppdK 885956 ppdK Mycobacterium tuberculosis H37Rv Probable pyruvate, phosphate dikinase PpdK NP_215643.1 1249932 R 83332 CDS NP_215644.1 15608268 885849 complement(1251617..1252972) 1 NC_000962.3 Rv1128c, (MTCY22G8.17c), len: 451 aa. Conserved hypothetical protein, in REP13E12 degenerate repeat, highly similar to several Mycobacterium tuberculosis proteins in REP13E12 repeats e.g. Rv1148c, Rv1945, Rv3467, etc.; hypothetical protein 1252972 885849 Rv1128c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215644.1 1251617 R 83332 CDS NP_215645.1 15608269 885963 complement(1253074..1254534) 1 NC_000962.3 Rv1129c, (MTCY22G8.18c), len: 486 aa. Possible transcriptional regulator protein, similar to Rv0465c|MTV038.09c Mycobacterium tuberculosis (474 aa),FASTA scores: E(): 0, (47.4% identity in 468 aa overlap). Helix turn helix motif present from aa 32-53.; Probable transcriptional regulator protein 1254534 885963 Rv1129c Mycobacterium tuberculosis H37Rv Probable transcriptional regulator protein NP_215645.1 1253074 R 83332 CDS NP_215646.1 15608270 885843 1254555..1256135 1 NC_000962.3 Rv1130, (MTCY22G8.19), len: 526 aa. Possible prpD,methylcitrate dehydratase (MCD), some similarity to AP000063|AP000063_192 hypothetical protein from Aeropyrum pernix (479 aa), FASTA scores: opt: 717, E(): 0, (34.3% identity in 443 a a overlap), and to PRPD_ECOLI|P77243 prpd protein from Escherichia coli (483aa), FASTA scores: opt: 234, E(): 3.3e-08, (27.0% identity in 429 aa overlap). Predicted possible vaccine candidate (See Zvi et al.,2008).; Possible methylcitrate dehydratase PrpD 1256135 prpD 885843 prpD Mycobacterium tuberculosis H37Rv Possible methylcitrate dehydratase PrpD NP_215646.1 1254555 D 83332 CDS NP_215647.1 15608271 888949 1256132..1257313 1 NC_000962.3 Rv1131, (MTCY22G8.20), len: 393 aa. Probable prpC,methylcitrate synthase (MCS) (previously known as gltA1) ,highly similar to CISY_MYCSM|P26491 citrate synthase from Mycobacterium smegmatis (375 aa), FASTA scores: opt:1942,E(): 0, (80.0% identity in 375 aa overlap). Also similar to two other M. tuberculosis citrate synthases,Rv0896c|MTCY31.24|gltA2 (431 aa), FASTA score: (33.1% identity in 381 aa overlap) and Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (31.8% identity in 371 aa overlap). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family.; Probable methylcitrate synthase PrpC 1257313 prpC 888949 prpC Mycobacterium tuberculosis H37Rv Probable methylcitrate synthase PrpC NP_215647.1 1256132 D 83332 CDS NP_215648.1 15608272 885824 1257325..1259055 1 NC_000962.3 Rv1132, (MTCY22G8.21), len: 576 aa. Conserved membrane protein, similar to O06827|Rv1431|MTCY493.23C membrane protein from Mycobacterium tuberculosis (589 aa),fasta scores: opt: 1811, E(): 0, (48.2% identity in 585 aa overlap).; hypothetical protein 1259055 885824 Rv1132 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215648.1 1257325 D 83332 CDS NP_215649.1 15608273 888947 complement(1259067..1261346) 1 NC_000962.3 Rv1133c, (MTC22G8.22), len: 759 aa (start site chosen by homology). Probable metE,5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, highly similar to others e.g. METE_ECOLI|P25665 Escherichia coli (752 aa), FASTA scores: opt: 2251, E(): 0, (48.1% identity in 756 aa overlap). Equivalent to Z94723|MLCB33_14 metE from M. leprae (760 aa) (85.3% identity in 755 aa overlap). Belongs to the vitamin-B12 independent methionine synthase family.; Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase MetE (methionine synthase, vitamin-B12 independent isozyme) 1261346 metE 888947 metE Mycobacterium tuberculosis H37Rv Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase MetE (methionine synthase, vitamin-B12 independent isozyme) NP_215649.1 1259067 R 83332 CDS NP_215650.1 15608274 885983 1261922..1262158 1 NC_000962.3 Rv1134, (MTCI65.01), len: 78 aa. Hypothetical unknown protein.; Hypothetical protein 1262158 885983 Rv1134 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215650.1 1261922 D 83332 CDS YP_177790.1 57116833 885131 complement(1262272..1264128) 1 NC_000962.3 Rv1135c, (MTCI65.02c), len: 618 aa. PPE16, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins. Similar to Rv2356c (59.6% identity in 627 aa overlap); etc.. Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE16 1264128 PPE16 885131 PPE16 Mycobacterium tuberculosis H37Rv PPE family protein PPE16 YP_177790.1 1262272 R 83332 CDS YP_177640.1 57116834 3205047 1264314..1264556 1 NC_000962.3 Rv1135A, len: 80 aa. Possible acetyl-CoA acetyltransferase (possible gene fragment), highly similar to other acetyl-CoA acetyltransferases e.g. C-terminal part of Rv3556c|Z92774|MTCY6G11_2|MTCY06G11.03|fadA6 acetyl-CoA acetyltransferase from Mycobacterium tuberculosis (386 aa),FASTA scores: opt: 219, E(): 5.7e-09, (63.6% identity in 55 aa overlap).; Possible acetyl-CoA acetyltransferase (acetoacetyl-CoA thiolase) 1264556 3205047 Rv1135A Mycobacterium tuberculosis H37Rv Possible acetyl-CoA acetyltransferase (acetoacetyl-CoA thiolase) YP_177640.1 1264314 D 83332 CDS NP_215652.1 15608276 888948 1264606..1264947 1 NC_000962.3 Rv1136, (MTCI65.03), len: 113 aa. Probable enoyl-CoA hydratase (possible gene fragment). Some similarity to N-terminus of carnitine racemases and enoyl-CoA hydratases (but much shorter) e.g. I41014 carnitine racemase from Escherichia coli (297 aa), FASTA scores: opt: 258, E(): 2.5e-11, (44.5% identity in 110 aa overlap); and Rv0222 putative enoyl-CoA hydratase from M. tuberculosis (262 aa).; Possible enoyl-CoA hydratase 1264947 888948 Rv1136 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase NP_215652.1 1264606 D 83332 CDS NP_215653.1 15608277 885071 complement(1265087..1265455) 1 NC_000962.3 Rv1137c, (MTCI65.04c), len: 122 aa. Hypothetical unknown protein.; Hypothetical protein 1265455 885071 Rv1137c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215653.1 1265087 R 83332 CDS NP_215654.1 15608278 885119 complement(1265472..1266488) 1 NC_000962.3 Rv1138c, (MTCI65.05c), len: 338 aa. Possible oxidoreductase, similar to Q9EWQ8 putative oxidoreductase from Streptomyces coelicolor (343 aa). Also similar to many Mycobacterium tuberculosis hypothetical proteins e.g. Rv1751|P72008|MTCY04C12.35 (412 aa), fasta scores: opt: 89,E(): 4.5e-09, (24.6% identity in 358 aa overlap).; Possible oxidoreductase 1266488 885119 Rv1138c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_215654.1 1265472 R 83332 CDS NP_215655.1 15608279 885110 complement(1266485..1266985) 1 NC_000962.3 Rv1139c, (MTCI65.06c), len: 166 aa. Conserved hypothetical membrane protein. Highly similar to P54158|YBPQ_BACSU hypothetical Bacillus subtilis protein,YBPQ (168 aa), FASTA scores: opt: 446, E(): 2.2e-26, (38.4% identity in 164 aa overlap). Some similarity to Mycobacterium tuberculosis hypothetical proteins, Rv0740,Rv0750.; hypothetical protein 1266985 885110 Rv1139c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215655.1 1266485 R 83332 CDS NP_215656.1 15608280 885117 1267347..1268195 1 NC_000962.3 Rv1140, (MTCI65.07), len: 282 aa. Probable integral membrane protein. Weak similarity in C-terminus to hypothetical Escherichia coli proteins YPRA and YPRB,possibly membrane-bound e.g. YPRA_ECOLI hypothetical 24.3 kDa protein (URF 1) (217 aa), FASTA scores: opt: 166, E(): 0.00062, (31.0% identity in 158 aa overlap).; Probable integral membrane protein 1268195 885117 Rv1140 Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_215656.1 1267347 D 83332 CDS NP_215657.1 15608281 886024 complement(1268203..1269009) 1 NC_000962.3 Rv1141c, (MTCI65.08c), len: 268 aa. Probable echA11,enoyl-CoA hydratase, similar to others e.g. P24162|ECHH_RHOCA probable enoyl-CoA hydratase from Rhodobacter capsulatus(257 aa); CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 513, E():1e-25, (36.1% identity in 249 aa overlap); etc. Also similarity with naphthoate synthases. Also highly similar to downstream ORF Rv1142c|MTCI65.09|echA10 probable enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa), FASTA scores: opt: 1225, E(): 0, (72.3% identity in 267 aa overlap).; Probable enoyl-CoA hydratase EchA11 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 1269009 echA11 886024 echA11 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA11 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215657.1 1268203 R 83332 CDS NP_215658.1 15608282 885458 complement(1269152..1269958) 1 NC_000962.3 Rv1142c, (MTCI65.09c), len: 268 aa. Probable echA10,enoyl-CoA hydratase, similar to others e.g. CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 525, E(): 1.3e-26, (35.1% identity in 251 aa overlap); NP_420658.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (267 aa); NP_438092.1|NC_003078 putative enoyl-CoA hydratase protein from Sinorhizobium meliloti (263 aa); etc. Also similarity with naphthoate synthases. Also highly similar to upstream ORF Rv1141c|MTCI65.08c|echA11 probable enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa), FASTA score: opt: 1225, E(): 0, (72.3% identity in 267 aa overlap).; Probable enoyl-CoA hydratase EchA10 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 1269958 echA10 885458 echA10 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA10 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215658.1 1269152 R 83332 CDS NP_215659.1 15608283 885067 1270062..1271144 1 NC_000962.3 Rv1143, (MTCI65.10), len: 360 aa. Probable mcr,alpha-methylacyl-CoA racemase. Strong similarity to other alpha-methylacyl-CoA racemases and also some similarity to L-carnitine dehydratase e.g. U89905|g1552373 methylacyl-CoA racemase alpha from Norway rat (361 aa), FASTA scores: opt: 1035, E():0, (47.2% identity in 339 aa overlap). Equivalent to (but longer than) Z94723|MLCB33_13 Mycobacterium leprae (253 aa) (85.3% identity in 245 aa overlap). Also similar to Mycobacterium tuberculosis putative racemases Rv0855,Rv1866, Rv3272.; Probable alpha-methylacyl-CoA racemase Mcr (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase ) 1271144 mcr 885067 mcr Mycobacterium tuberculosis H37Rv Probable alpha-methylacyl-CoA racemase Mcr (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase ) NP_215659.1 1270062 D 83332 CDS NP_215660.1 15608284 885932 1271156..1271908 1 NC_000962.3 Rv1144, (MTCI65.11), len: 250 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases e.g. NP_104056.1|NC_002678 3-hydroxyacyl-CoA dehydrogenase type II from Mesorhizobium loti (253 aa); NP_251244.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (255 aa); AAK15008.1|AF233685_1|AF233685 short chain L-3-hydroxyacyl-CoA dehydrogenase from Mus musculus (261 aa); HSU73514|g1778354|XH98G2 human short-chain alcohol dehydrogenase from Homo sapiens (261 aa), FASTA scores: opt: 875, E(): 0, (60.1% identity in 253 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 1271908 885932 Rv1144 Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_215660.1 1271156 D 83332 CDS NP_215661.1 15608285 885798 1272423..1273334 1 NC_000962.3 Rv1145, (MTCI65.12), len: 303 aa. Probable mmpL13a,conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. MTCY987|g1781238 from Mycobacterium tuberculosis (962 aa), FASTA scores: opt: 213, E(): 1.9e-06, (28.0% identity in 296 aa overlap); etc. Strong similarity to U92075|MMU92075_5 hypothetical protein from Mycobacterium marinum (256 aa), FASTA scores: opt: 957,E(): 0, (57.6% identity in 257 aa overlap). Should continue as mmpL13B|Rv1146, but frameshift required. Sequence has been checked and is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL13a 1273334 mmpL13a 885798 mmpL13a Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL13a NP_215661.1 1272423 D 83332 CDS NP_215662.1 15608286 885575 1273355..1274767 1 NC_000962.3 Rv1146, (MTCI65.13), len: 470 aa. Probable mmpL13b,conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. Q53902|C40046 antibiotic transport-associated protein from Streptomyces coelicolor (711 aa), FASTA scores: opt: 193, E(): 2.1e-05, (28.9% identity in 394 aa overlap); etc. Could be in frame with previous ORF mmpL13A|Rv1145, but no sequence error apparent to account for this; sequence is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL13b 1274767 mmpL13b 885575 mmpL13b Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL13b NP_215662.1 1273355 D 83332 CDS NP_215663.1 15608287 885471 1274900..1275550 1 NC_000962.3 Rv1147, (MTCI65.14), len: 216 aa. Conserved protein,similar to many conserved hypothetical proteins, and some similarity to several methyltransferases e.g. Q05197|PMTA_RHOSH phosphatidylethanolamine N-methyltransferase from R. sphaeroides (203 aa), FASTA scores: opt: 156, E(): 0.00073, (27.6% identity in 156 aa overlap).; hypothetical protein 1275550 885471 Rv1147 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215663.1 1274900 D 83332 CDS NP_215664.1 15608288 885451 complement(1276300..1277748) 1 NC_000962.3 Rv1148c, (MTCI65.15c), len: 482 aa. Conserved hypothetical ORF in REP13E12 degenerate repeat, nearly identical to other hypothetical Mycobacterium tuberculosis proteins in REP13E12 repeats, although similarity extends upstream past proposed f-Met start. Very similar to other REP13E12 proteins e.g. Rv1945, Rv3467, Rv0094c, Rv1128c etc.; hypothetical protein 1277748 885451 Rv1148c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215664.1 1276300 R 83332 CDS NP_215665.1 15608289 885164 1277893..1278300 1 NC_000962.3 Rv1149, (MTCI65.16), len: 135 aa. Possible transposase. Identical to 117 aa N-terminal region of S21394|X65618 transposase of Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1042c|MTCY10G2.07.; Possible transposase 1278300 885164 Rv1149 Mycobacterium tuberculosis H37Rv Possible transposase NP_215665.1 1277893 D 83332 CDS NP_215667.1 15608291 886026 complement(1278904..1279617) 1 NC_000962.3 Rv1151c, (MTCI65.18c), len: 237 aa. Transcriptional regulatory protein, similar to others AE000776|AE000776_10 Aquifex aeolicus (239 aa), FASTA scores: opt: 725, E(): 0,(46.4% identity in 237 aa overlap); ECAE0002125|g1787358 Escherichia coli (279 aa), FASTA scores: opt: 464, E(): 1.3e-23, (36.7% identity in 240 aa overlap).; Transcriptional regulatory protein 1279617 886026 Rv1151c Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein NP_215667.1 1278904 R 83332 CDS NP_215668.1 15608292 885985 1279655..1280020 1 NC_000962.3 Rv1152, (MTCI65.19), len: 121 aa (Start uncertain). Probable transcriptional regulatory protein, some similarity to others e.g. YHCF_BACSU hypothetical transcriptional regulator (121 aa), FASTA scores: opt: 187,E(): 1.9e-06, (34.9% identity in 106 aa overlap). Helix turn helix motif from aa 42-63 (+3.10 SD).; Probable transcriptional regulatory protein 1280020 885985 Rv1152 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215668.1 1279655 D 83332 CDS NP_215669.1 15608293 885994 complement(1279998..1280846) 1 NC_000962.3 Rv1153c, (MTCI65.20c), len: 282 aa. Probable omt,O-methyltransferase, similar to TCMP_STRGA|P39887 Tetracenomycin polyketide synthesis O-methyltransferase tcmP from Streptomyces glaucescens (270 aa), FASTA scores: opt: 368, E(): 1.7e-17, (31.3% identity in 233 aa overlap).; Probable O-methyltransferase Omt 1280846 omt 885994 omt Mycobacterium tuberculosis H37Rv Probable O-methyltransferase Omt NP_215669.1 1279998 R 83332 CDS NP_215670.1 15608294 885991 complement(1280843..1281484) 1 NC_000962.3 Rv1154c, (MTCI65.21c), len: 213 aa. Hypothetical unknown protein, start uncertain.; Hypothetical protein 1281484 885991 Rv1154c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215670.1 1280843 R 83332 CDS NP_215671.1 15608295 885604 1281429..1281872 1 NC_000962.3 Rv1155, (MTCI65.22), len: 147 aa. Possible pyridoxine 5'-phosphate oxidase (PNPOx) (See Biswal et al.,2005; Canaan et al., 2005). Similar to hypothetical proteins e.g. AL079356|SC6G9.20 Streptomyces coelicolor (144 aa), FASTA scores: opt: 478, E(): 2.8e-26, (55.7% identity in 140 aa overlap); and Mycobacterium tuberculosis proteins Rv1875, Rv0121c, Rv2074.; Possible pyridoxamine 5'-phosphate oxidase (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) 1281872 885604 Rv1155 Mycobacterium tuberculosis H37Rv Possible pyridoxamine 5'-phosphate oxidase (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) NP_215671.1 1281429 D 83332 CDS NP_215672.1 15608296 885593 1282306..1282893 1 NC_000962.3 Rv1156, (MTCI65.23), len: 195 aa. Conserved protein,highly similar to CAC32318.1|AL583944 conserved hypothetical protein from Streptomyces coelicolor (197 aa).; hypothetical protein 1282893 885593 Rv1156 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215672.1 1282306 D 83332 CDS NP_215673.1 15608297 885878 complement(1283056..1284171) 1 NC_000962.3 Rv1157c, (MTCI65.24c), len: 371 aa. Conserved Ala-,Pro-rich protein, similar to other proline rich proteins and extensins e.g. GBU04267|g451543 sea-island cotton proline-rich protein of cotton fiber (214 aa), FASTA scores: opt: 305, E(): 3.9e-05, (35.7% identity in 182 aa overlap). Has hydrophobic stretch at N-terminus suggestive of secretion signal. First start taken. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Conserved ala-, pro-rich protein 1284171 885878 Rv1157c Mycobacterium tuberculosis H37Rv Conserved ala-, pro-rich protein NP_215673.1 1283056 R 83332 CDS NP_215674.1 15608298 888930 complement(1284179..1284862) 1 NC_000962.3 Rv1158c, (MTCI65.25c), len: 227 aa. Conserved hypothetical Ala-, Pro-rich protein, similar to other proline rich proteins and extensins e.g. MMSAP62|g633250 house mouse (485 aa), FASTA scores: opt: 367, E(): 1.2e-08,(36.3% identity in 212 aa overlap). Has hydrophobic stretch at N-terminus suggestive of secretion signal. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Conserved hypothetical ala-, pro-rich protein 1284862 888930 Rv1158c Mycobacterium tuberculosis H37Rv Conserved hypothetical ala-, pro-rich protein NP_215674.1 1284179 R 83332 CDS NP_215675.1 15608299 885899 1284992..1286287 1 NC_000962.3 Rv1159, (MTCI65.26), len: 431 aa. PimE,mannosyltransferase (see Morita et al., 2006) Conserved transmembrane protein, similar to others in Mycobacterium tuberculosis e.g. Rv2181|MTCY21D4.13 (560 aa), FASTA scores: opt: 172; E(): 0.00035, (25.0% identity in 332 aa overlap). Belongs to the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003).; Mannosyltransferase PimE 1286287 pimE 885899 pimE Mycobacterium tuberculosis H37Rv Mannosyltransferase PimE NP_215675.1 1284992 D 83332 CDS YP_177641.1 57116835 3205109 complement(1286284..1286568) 1 NC_000962.3 Rv1159A, len: 94 aa. Unknown protein.; hypothetical protein 1286568 3205109 Rv1159A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177641.1 1286284 R 83332 CDS NP_215676.1 15608300 888485 1286595..1287020 1 NC_000962.3 Rv1160, (MTCI65.27), len: 141 aa. Probable mutT2,mutator protein or homolog (see citation below). More similar to D908197|g1742860 MutT homolog from Escherichia coli (135 aa), FASTA scores: opt: 226, E():1.1e-08, (39.7% identity in 116 aa overlap); than to MUTT_ECOLI|P08337 mutator mutt protein from Escherichia coli (129 aa), FASTA scores: opt: 180, E(): 1.2e-05, (27.1% identity in 129 aa overlap). Contains PS00893 mutT domain signature.; Probable mutator protein MutT2 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) 1287020 mutT2 888485 mutT2 Mycobacterium tuberculosis H37Rv Probable mutator protein MutT2 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) NP_215676.1 1286595 D 83332 CDS NP_215677.1 15608301 885573 1287328..1291026 1 NC_000962.3 Rv1161, (MTCI65.28), len: 1232 aa. narG, respiratory nitrate reductase alpha chain. Similar to others e.g. NARG_BACSU nitratereductase alpha chain from Bacillus subtilis (1228 aa), FASTA scores: opt: 4218, E(): 0, (50.3% identity in 1229 aa overlap); etc. Also highly similar to N-terminal part of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase from Mycobacterium tuberculosis (85.1% identity in 281 aa overlap). Contains prokaryotic molybdopterin oxidoreductase signatures 1 and 2 (PS00551,PS00490). Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.; Respiratory nitrate reductase (alpha chain) NarG 1291026 narG 885573 narG Mycobacterium tuberculosis H37Rv Respiratory nitrate reductase (alpha chain) NarG NP_215677.1 1287328 D 83332 CDS NP_215678.1 15608302 888265 1291065..1292741 1 NC_000962.3 Rv1162, (MTCI65.29), len: 558 aa. Probable narH,respiratory nitrate reductase beta chain. Similar to others e.g. NARH_BACSU|P42176 nitrate reductase beta chain from Bacillus subtilis (487 aa), FASTA scores: opt: 2049, E(): 0, (56.8% identity in 488 aa overlap); etc. Contains PS00190 cytochrome c family heme-binding site signature.; Probable respiratory nitrate reductase (beta chain) NarH 1292741 narH 888265 narH Mycobacterium tuberculosis H37Rv Probable respiratory nitrate reductase (beta chain) NarH NP_215678.1 1291065 D 83332 CDS NP_215679.1 15608303 885890 1292798..1293403 1 NC_000962.3 Rv1163, (MTCI65.30), len: 201 aa. Probable narJ,respiratory nitrate reductase delta chain. Similar to others e.g. P42178|NARJ_BACSU nitrate reductase delta chain from Bacillus subtilis (184 aa), FASTA scores: opt: 254,E(): 1.9e-10, (31.8% identity in 179 aa overlap); etc. Strong similarity to region from aa 260 - 410 of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase from Mycobacterium tuberculosis (64.8% identity in 159 aa overlap).; Probable respiratory nitrate reductase (delta chain) NarJ 1293403 narJ 885890 narJ Mycobacterium tuberculosis H37Rv Probable respiratory nitrate reductase (delta chain) NarJ NP_215679.1 1292798 D 83332 CDS NP_215680.1 15608304 888935 1293406..1294146 1 NC_000962.3 Rv1164, (MTCI65.31), len: 246 aa. Probable narI,respiratory nitrate reductase gamma chain. Similar to others e.g. NARI_BACSU|P42177 nitrate reductase gamma chain from Bacillus subtilis (223 aa), FASTA scores: opt: 652,E(): 0; (41.6% identity in 221 aa overlap); etc. Highly similar to C-terminal part of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase (gamma chain) from Mycobacterium tuberculosis (68.6% identity in 239 aa overlap).; Probable respiratory nitrate reductase (gamma chain) NarI 1294146 narI 888935 narI Mycobacterium tuberculosis H37Rv Probable respiratory nitrate reductase (gamma chain) NarI NP_215680.1 1293406 D 83332 CDS NP_215681.1 15608305 886038 1294168..1296054 1 NC_000962.3 Rv1165, (MTV005.01-MTCI65.32), len: 628 aa. Possible typA (alternate gene name: bipA), GTP-binding translation elongation factor, similar to several e.g. P32132|TYPA_ECOLI|BIPA|B387 Escherichia coli (591 aa); YIHK_SYNY3|P72749 gtp-binding protein TYPA/BIPA homolog from synechocystis sp. (597 aa), FASTA scores: E(): 0,(46.9% identity in 610 aa overlap); and to elongation factor EF-G from many organims e.g. EFG_MICLU|P09952 micrococcus luteus (701 aa), FASTA scores: E(): 3e-24,(29.8% identity in 500 aa overlap). Belongs to the GTP-binding elongation factor family, TYPA subfamily.; Possible GTP-binding translation elongation factor TypA (tyrosine phosphorylated protein A) (GTP-binding protein) 1296054 typA 886038 typA Mycobacterium tuberculosis H37Rv Possible GTP-binding translation elongation factor TypA (tyrosine phosphorylated protein A) (GTP-binding protein) NP_215681.1 1294168 D 83332 CDS NP_215682.1 15608306 886036 1296152..1298059 1 NC_000962.3 Rv1166, (MTV005.02), len: 635 aa. Probable lpqW,conserved lipoprotein, almost identical in part to G2384665|AF009358 Mycobacterium tuberculosis gene fragment ORFA2-898 (fragment) (59 aa) (93.9% identity in 49 aa overlap) (see * below). Also similar to Rv1280c and Rv2585c. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. [* Note: Unpublished. Identification of Mycobacterium tuberculosis peptides that stimulate immune human peripheral blood monocytes. Nano F.E., Doran J.L., Treit J.D., Moran A.J.]; Probable conserved lipoprotein LpqW 1298059 lpqW 886036 lpqW Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqW NP_215682.1 1296152 D 83332 CDS NP_215683.1 15608307 888758 complement(1298087..1298692) 1 NC_000962.3 Rv1167c, (MTV005.03c), len: 201 aa. Probable transcriptional regulator, similar to several e.g. D1022772|D85417 hemR from Propionibacterium freudenreichii (243 aa), FASTA scores: opt: 268, E(): 5.4e-16, (35.9% identity in 198 aa overlap) and AL022268|SC4H2.32 Streptomyces coelicolor (111 aa), FASTA scores: opt: 274,E(): 5e-11, (55.1% identity in 89 aa overlap).; Probable transcriptional regulatory protein 1298692 888758 Rv1167c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215683.1 1298087 R 83332 CDS YP_177791.1 57116836 885990 complement(1298764..1299804) 1 NC_000962.3 Rv1168c, (MTV005.04c), len: 346 aa. PPE17, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. E332789|Z98268|MTCI125.27C (385 aa), FASTA scores: opt: 504, E(): 0, (36.6% identity in 388 aa overlap).; PPE family protein PPE17 1299804 PPE17 885990 PPE17 Mycobacterium tuberculosis H37Rv PPE family protein PPE17 YP_177791.1 1298764 R 83332 CDS YP_177792.1 57116837 885930 complement(1299822..1300124) 1 NC_000962.3 Rv1169c, (MTV005.05c), len: 100 aa. Possible lipX,lipase. Member of the Mycobacterium tuberculosis PE family of proteins (see Brennan & Delogu 2002), e.g. O05297|Z93777|MTCI364.07 (99 aa), FASTA scores: opt: 209,E(): 1.6e-15, (37.4% identity in 99 aa overlap). Also simlar to the N-terminus of P77909|U76006 esterase/lipase from Mycobacterium tuberculosis (437 aa), FASTA scores: opt: 193, E(): 4.4e-14, (37.2% identity in 94 aa overlap). Contains a helix-turn-helix motif from aa 88-109 (+2.76 SD). Predicted possible vaccine candidate (See Zvi et al.,2008).; PE family protein Possible lipase LipX 1300124 lipX 885930 lipX Mycobacterium tuberculosis H37Rv PE family protein Possible lipase LipX YP_177792.1 1299822 R 83332 CDS NP_215686.1 15608310 885997 1300304..1301215 1 NC_000962.3 Rv1170, (MTV005.06), len: 303 aa. MshB, N-Acetyl-1-D-myo-Inosityl-2-Amino-2-Deoxy-alpha-D- Glucopyranoside Deacetylase (GlcNAc-Ins deacetylase) (see citation below),similar to Q54358|X79146 lmbE gene from Streptomyces lincolnensis (270 aa), FASTA scores: opt: 308, E(): 1.2e-15, (32.0% identity in 278 aa overlap). Also similar to Rv1082|MCA Mycothiol conjugate amidase from Mycobacterium tuberculosis (288 aa).; N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha- D-glucopyranoside deacetylase MshB (GlcNAc-Ins deacetylase) 1301215 mshB 885997 mshB Mycobacterium tuberculosis H37Rv N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha- D-glucopyranoside deacetylase MshB (GlcNAc-Ins deacetylase) NP_215686.1 1300304 D 83332 CDS NP_215687.2 57116838 885986 1301307..1301747 1 NC_000962.3 Rv1171, (MTV005.07), len: 146 aa. Conserved hypothetical protein, possibly transmembrane protein. Start has been changed since first submission.; hypothetical protein 1301747 885986 Rv1171 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215687.2 1301307 D 83332 CDS YP_177793.1 57116839 885988 complement(1301755..1302681) 1 NC_000962.3 Rv1172c, (MTV005.08c), len: 308 aa. PE12, Member of the Mycobacterium tuberculosis PE family of proteins (see Brennan & Delogu 2002), e.g. P71748|Z81368|MTCY253.25C (361 aa), FASTA scores: opt: 483, E(): 7.8e-22, (46.4% identity in 192 aa overlap).; PE family protein PE12 1302681 PE12 885988 PE12 Mycobacterium tuberculosis H37Rv PE family protein PE12 YP_177793.1 1301755 R 83332 CDS NP_215689.1 15608313 886061 1302931..1305501 1 NC_000962.3 Rv1173, (MTV005.09), len: 856 aa. Probable fbiC,F420 biosynthesis protein, equivalent to AAL91922|FBIC F420 biosynthesis protein fbiC from Mycobacterium bovis BCG (856 aa) (see citation below). The N-terminus (aa 80-420) is similar to Y446_METJA|Q57888 hypothetical protein mj0446 from methanococcus jannaschii (361 aa), FASTA scores: opt: 801, E(): 0, (41.2% identity in 337 aa overlap); and the C-terminus region (aa 530-856) is similar to e.g. YE31_METJA|Q58826 hypothetical protein mj1431 from methanococcus jannaschii (359 aa), FASTA scores: opt: 1089,E(): 0, (48.7% identity in 337 aa overlap).; Probable F420 biosynthesis protein FbiC 1305501 fbiC 886061 fbiC Mycobacterium tuberculosis H37Rv Probable F420 biosynthesis protein FbiC NP_215689.1 1302931 D 83332 CDS NP_215690.1 15608314 886082 complement(1305669..1306001) 1 NC_000962.3 Rv1174c, (MTV005.10c), len: 110 aa. TB8.4, low molecular weight T-cell antigen (see citations below),hypothetical unknown secreted protein. Predicted to be an outer membrane protein (See Song et al., 2008). Predicted possible vaccine candidate (See Zvi et al., 2008).; Low molecular weight T-cell antigen TB8.4 1306001 TB8.4 886082 TB8.4 Mycobacterium tuberculosis H37Rv Low molecular weight T-cell antigen TB8.4 NP_215690.1 1305669 R 83332 CDS NP_215691.1 15608315 886053 complement(1306202..1308226) 1 NC_000962.3 Rv1175c, (MTV005.11c), len: 674 aa. Probable fadH,NADPH-dependent 2,4-dienoyl-CoA reductase, highly similar to others e.g. NP_251782.1|NC_002516 2,4-dienoyl-CoA reductase FadH1 from Pseudomonas aeruginosa (679 aa); CAC01564.1|AL391039 2,4-dienoyl-CoA reductase [NADPH] from Streptomyces coelicolor (671 aa); P42593|FADH_ECOLI 2,4-dienoyl-CoA reductase from Escherichia coli (671 aa),FASTA scores: opt: 2344, E(): 0, (53.1% identity in 671 aa overlap); etc. Also similar to Rv3359|MTV004.16 putative oxidoreductase from Mycobacterium tuberculosis (396 aa).; Probable NADPH dependent 2,4-dienoyl-CoA reductase FadH (2,4-dienoyl coenzyme A reductase) (4-enoyl-CoA reductase) 1308226 fadH 886053 fadH Mycobacterium tuberculosis H37Rv Probable NADPH dependent 2,4-dienoyl-CoA reductase FadH (2,4-dienoyl coenzyme A reductase) (4-enoyl-CoA reductase) NP_215691.1 1306202 R 83332 CDS NP_215692.1 15608316 886080 complement(1308223..1308792) 1 NC_000962.3 Rv1176c, (MTV005.12c), len: 189 aa. Conserved hypothetical protein, some similarity to P94443|D78508 hypothetical protein from Bacillus subtilis (182 aa), FASTA scores: opt: 219, E(): 1.7e-15, (25.1% identity in 183 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv0047c.; hypothetical protein 1308792 886080 Rv1176c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215692.1 1308223 R 83332 CDS NP_215693.1 15608317 885869 1309005..1309331 1 NC_000962.3 Rv1177, (MTV005.13), len: 108 aa. Probable fdxC,ferredoxin, equivalent to NP_302047.1|NC_002677 ferredoxin from Mycobacterium leprae (108 aa); P00215|FER_MYCSM ferredoxin from Mycobacterium smegmatis (106 aa), FASTA scores: opt: 705, E(): 0, (87.7% identity in 106 aa overlap). Also highly similar to many e.g. JH0239 ferredoxin precursor from Saccharopolyspora erythraea (105 aa); P24496|FER_SACER ferredoxin from Saccharopolyspora erythraea (106 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Belongs to the bacterial type ferredoxin family. Cofactor: binds 1 4FE-4S cluster and a 3FE-4S cluster (by similarity).; Probable ferredoxin FdxC 1309331 fdxC 885869 fdxC Mycobacterium tuberculosis H37Rv Probable ferredoxin FdxC NP_215693.1 1309005 D 83332 CDS NP_215694.1 15608318 886031 1309364..1310452 1 NC_000962.3 Rv1178, (MTV005.14), len: 362 aa. Probable aminotransferase, weak similarity to many aspartate aminotransferases e.g. Q55679|D64000 SLL0006 aspartate aminotransferase from Synechocystis sp. (394 aa), FASTA scores: opt: 218, E(): 1.3e-25, (32.5% identity in 379 aa overlap). Contains PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site. Also similar to Mycobacterium tuberculosis aminotransferases Rv2294,Rv0075, etc.; Probable aminotransferase 1310452 886031 Rv1178 Mycobacterium tuberculosis H37Rv Probable aminotransferase NP_215694.1 1309364 D 83332 CDS NP_215695.1 15608319 886083 complement(1310480..1313299) 1 NC_000962.3 Rv1179c, MTV005.15c, len: 939 aa. Unknown protein.; hypothetical protein 1313299 886083 Rv1179c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215695.1 1310480 R 83332 CDS NP_215696.1 15608320 886055 1313725..1315191 1 NC_000962.3 Rv1180, (MTV005.16), len: 488 aa. Probable polyketide beta-ketoacyl synthase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to the N-terminus of many polyketide synthases e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 2115, E(): 0, (66.5% identity in 472 aa overlap). Also similar to, and same length as P96284|Z83858|MTCY24G1.02 M. tuberculosis (496 aa), FASTA scores: opt: 1424, E(): 0, (50.9% identity in 444 aa overlap). Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site,also PS00606 Beta-ketoacyl synthases active site. Belongs to the beta-ketoacyl-ACP synthases family. Alternative nucleotide at position 1315191 (a->C; Stop489Y) has been observed. Rv1180/Rv1181 fusion has been called msl3.; Probable polyketide beta-ketoacyl synthase Pks3 1315191 pks3 886055 pks3 Mycobacterium tuberculosis H37Rv Probable polyketide beta-ketoacyl synthase Pks3 NP_215696.1 1313725 D 83332 CDS NP_215697.1 15608321 886081 1315234..1319982 1 NC_000962.3 Rv1181, (MTV005.17), len: 1582 aa. Probable pks4,polyketide synthase, similar to many e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 3518, E(): 0, (59.7% identity in 1614 aa overlap). Note that this similarity extends upstream of the first initiation codon into the upstream MTV005.16; the stop codon at the end of MTV005.16 is present in at least 4 independent clones (BAC, cosmid and pUC) from the genome (however an alternative nucleotide at position 1315191 (a->C; Stop489Y) has also been observed). The two CDS's may represent separate modules of the polyketide synthase. Rv1180/Rv1181 fusion has been called msl3.; Probable polyketide beta-ketoacyl synthase Pks4 1319982 pks4 886081 pks4 Mycobacterium tuberculosis H37Rv Probable polyketide beta-ketoacyl synthase Pks4 NP_215697.1 1315234 D 83332 CDS NP_215698.1 15608322 886072 1320035..1321453 1 NC_000962.3 Rv1182, (MTV005.18), len: 472 aa. Probable papA3,conserved polyketide synthase (PKS) associated protein,similar to other Mycobacterial hypothetical proteins e.g. Q49618|U00010 B1170_C1_180 from Mycobacterium leprae (471 aa), FASTA scores: opt: 2526, E(): 0, (75.6% identity in 471 aa overlap). Similar to other Mycobacterium tuberculosis hypothetical papA proteins; Rv3824c, Rv3820c,Rv1528c.; Probable conserved polyketide synthase associated protein PapA3 1321453 papA3 886072 papA3 Mycobacterium tuberculosis H37Rv Probable conserved polyketide synthase associated protein PapA3 NP_215698.1 1320035 D 83332 CDS NP_215699.1 15608323 886068 1321520..1324528 1 NC_000962.3 Rv1183, (MTV005.19), len: 1002 aa. Probable mmpL10,conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, similar to many Mycobacterial hypothetical membrane proteins e.g. Q49619|U00010 from Mycobacterium leprae (1008 aa), FASTA scores: opt: 4545, E(): 0, (70.6% identity in 978 aa overlap); etc. Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL10 1324528 mmpL10 886068 mmpL10 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL10 NP_215699.1 1321520 D 83332 CDS NP_215700.1 15608324 886063 complement(1324532..1325611) 1 NC_000962.3 Rv1184c, (MTV005.20c), len: 359 aa. Possible exported protein with potential N-terminal signal sequence. Similar to several Mycobacterial hypothetical proteins e.g. Q49633|U00010 Protein B1170_F3_112 from M. leprae (391 aa),FASTA scores: opt: 1422, E(): 0, (62.7% identity in 338 aa overlap). Also similar to Rv3822, Rv3539, Rv1430, Rv0151c,etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible exported protein 1325611 886063 Rv1184c Mycobacterium tuberculosis H37Rv Possible exported protein NP_215700.1 1324532 R 83332 CDS NP_215701.1 15608325 886065 complement(1325776..1327512) 1 NC_000962.3 Rv1185c, (MTV005.21c), len: 578 aa. Probable fadD21,fatty-acid-AMP synthetase, highly similar to several from Mycobacteria e.g. NP_301895.1|NC_002677 possible acyl-CoA synthase from Mycobacterium leprae (579 aa); P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2388, E(): 0, (61.8% identity in 579 aa overlap); etc. Seems to belong to the ATP-dependent AMP-binding enzyme family. Nucleotide position 1327402 in the genome sequence has been corrected, T:C resulting in E37E.; Probable fatty-acid-AMP ligase FadD21 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 1327512 fadD21 886065 fadD21 Mycobacterium tuberculosis H37Rv Probable fatty-acid-AMP ligase FadD21 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_215701.1 1325776 R 83332 CDS NP_215702.1 15608326 886057 complement(1327689..1329305) 1 NC_000962.3 Rv1186c, (MTV005.22c), len: 538 aa. Conserved protein, similar to AL117385|SC5G9.24 hypothetical protein from Streptomyces coelicolor (555 aa), FASTA scores: opt: 485, E(): 2.3e-23, (32.6% identity in 568 aa overlap). Contains helix turn helix motif from aa 488-509 (+2.81 SD).; hypothetical protein 1329305 886057 Rv1186c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215702.1 1327689 R 83332 CDS NP_215703.1 15608327 886058 1329390..1331021 1 NC_000962.3 Rv1187, (MTV005.23), len: 543 aa. Probable rocA,pyrroline-5-carboxylate dehydrogenase, similar to many e.g. PUT2_HUMAN|P30038 human delta-1-pyrroline-5-carboxylate dehydrogenase (563 aa), FASTA scores: opt: 1596, E():0,(46.0% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis hypothetical dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site.; Probable pyrroline-5-carboxylate dehydrogenase RocA 1331021 rocA 886058 rocA Mycobacterium tuberculosis H37Rv Probable pyrroline-5-carboxylate dehydrogenase RocA NP_215703.1 1329390 D 83332 CDS NP_215704.1 15608328 886054 1331021..1332010 1 NC_000962.3 Rv1188, (MTV005.24), len: 329 aa. Possible putA,proline dehydrogenase, similar to part of Q52711|X78346 proline dehydrogenase from Rhodobacter capsulatus (1127 aa), FASTA scores: opt: 194, E(): 1.5e-07, (31.2% identity in 349 aa overlap). Also similar to two Bacillus subtilis proline dehydrohenases E1184363|Z99120 (302 aa), FASTA scores: opt: 509, E(): 0, (37.1% identity in 313 aa overlap); and E1182272|Z99105 (303 aa), FASTA scores: opt: 513, E(): 0, (32.5% identity in 311 aa overlap). Highly similar to AL035569|SC8D9.31 Streptomyces coelicolor (308 aa), FASTA scores: opt: 984, E(): 0, (50.0% identity in 312 aa overlap). Nucleotide position 1331696 in the genome sequence has been corrected, A:C resulting in R226R.; Probable proline dehydrogenase 1332010 886054 Rv1188 Mycobacterium tuberculosis H37Rv Probable proline dehydrogenase NP_215704.1 1331021 D 83332 CDS NP_215705.1 15608329 886079 1332092..1332964 1 NC_000962.3 Rv1189, (MTV005.25-MTCI364.01), len: 290 aa. Possible sigI, alternative RNA polymerase sigma factor (see Gomez et al., 1997; Chen et al., 2000), similar to several e.g. O05767|U87307 extracytoplasmic function alternative sigma factor (sigE) from Mycobacterium smegmatis (204 aa),FASTA scores: opt: 239, E(): 1.3e-09, (32.9% identity in 167 aa overlap).; Possible alternative RNA polymerase sigma factor SigI 1332964 sigI 886079 sigI Mycobacterium tuberculosis H37Rv Possible alternative RNA polymerase sigma factor SigI NP_215705.1 1332092 D 83332 CDS NP_215706.1 15608330 886049 1332980..1333858 1 NC_000962.3 Rv1190, (MTCI364.02), len: 292 aa. Conserved hypothetical protein, similar to Rv1833c|Y0DA_MYCTU|Q50600 hypothetical 32.2 kDa protein cy1a11.10 (286 aa), fasta scores: opt: 331, E(): 1.4e-15, (29.0% identity in 272 aa overlap), also YU14_MYCTU|Q50670 putative haloalkane dehalogenase (300 aa), FASTA scores: opt: 239, E(): 2.2e-09, (29.9% identity in 298 aa overlap).; hypothetical protein 1333858 886049 Rv1190 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215706.1 1332980 D 83332 CDS NP_215707.1 15608331 886041 1333931..1334845 1 NC_000962.3 Rv1191, (MTCI364.03), len: 304 aa. Conserved protein, similar to Q54528 RDMC from Streptomyces purpurascens (298 aa), FASTA scores: opt: 196, E(): 1.5e-05, (27.5% identity in 269 aa overlap); Rv0134|MTCI5.08 (300 aa), FASTA scores: opt: 197, E(): 6.6e-06, (26.4% identity in 299 aa overlap), some similarity to PIP_NEIGO|P42786 proline iminopeptidase (310 aa), FASTA scores: opt: 196, E(): 1.3e-05, (32.2% identity in 152 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature.; hypothetical protein 1334845 886041 Rv1191 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215707.1 1333931 D 83332 CDS NP_215708.1 15608332 886007 1334927..1335754 1 NC_000962.3 Rv1192, (MTCI364.04), len: 275 aa. Unknown protein,contains PS00120 lipases, serine active site.; hypothetical protein 1335754 886007 Rv1192 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215708.1 1334927 D 83332 CDS NP_215709.1 15608333 886074 1335794..1337215 1 NC_000962.3 Rv1193, (MTCI364.05), len: 473 aa. Probable fadD36,fatty-acid-CoA synthetase, highly similar to Q50017|U15181 4-coumarate-CoA ligase from Mycobacterium leprae (476 aa),FASTA scores: opt: 2594, E(): 0, (81.3% identity in 476 aa overlap). Also highly similar to others e.g. CAB86109.1|AL163003 putative fatty acid synthase from Streptomyces coelicolor (485 aa); LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 605, E(): 8.4e-30, (33.0% identity in 364 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Probable fatty-acid-CoA ligase FadD36 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 1337215 fadD36 886074 fadD36 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD36 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_215709.1 1335794 D 83332 CDS NP_215710.1 15608334 886040 complement(1337248..1338513) 1 NC_000962.3 Rv1194c, (MTCI364.06c), len: 421 aa. Conserved protein, highly similar to Q50018 possible transcriptional activator from Mycobacterium leprae (517 aa), FASTA scores: opt: 1960, E(): 0, (69.8% identity in 421 aa overlap). Also similar to Mycobacterium tuberculosis Rv2370c|MTCY27.10,(62.0% identity in 421 aa overlap) and Rv1453|MTCY493.01c.; hypothetical protein 1338513 886040 Rv1194c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215710.1 1337248 R 83332 CDS YP_177794.1 57116840 886044 1339003..1339302 1 NC_000962.3 Rv1195, (MTCI364.07), len: 99 aa. PE13, Member of Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), e.g. Y0DP_MYCTU|Q50615 hypothetical glycine-rich 40.8 kd protein (498 aa), FASTA scores: opt: 307, E(): 1.4e-12, (56.3% identity in 96 aa overlap), similar to MTCY21C12.10c (99 aa), FASTA scores: opt:295, E(): 1.9e-11,(51.5% identity in 97 aa overlap).; PE family protein PE13 1339302 PE13 886044 PE13 Mycobacterium tuberculosis H37Rv PE family protein PE13 YP_177794.1 1339003 D 83332 CDS YP_177795.1 57116841 886073 1339349..1340524 1 NC_000962.3 Rv1196, (MTCI364.08), len: 391 aa. PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124,E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see Dillon et al., 1999).; PPE family protein PPE18 1340524 PPE18 886073 PPE18 Mycobacterium tuberculosis H37Rv PPE family protein PPE18 YP_177795.1 1339349 D 83332 CDS NP_215713.1 15608337 886051 1340659..1340955 1 NC_000962.3 Rv1197, (MT1235, MTCI364.09), len: 98 aa. EsxK,ESAT-6 like protein (see citation below). Member of M. tuberculosis hypothetical QILSS protein family with Rv1038c, etc. Almost identical to MTCY98.023c (98 aa) (99.0% identity in 98 aa overlap) and MTCY10G2.11 (98 aa),FASTA scores: opt: 643, E(): 0, (99.0% identity in 98 aa overlap); highly similar to Q49945|U1756C from Mycobacterium leprae (100 aa), FASTA scores: opt: 377, E(): 8e-21, (58.3% identity in 96 aa overlap). Belongs to the ESAT6 family.; ESAT-6 like protein EsxK (ESAT-6 like protein 3) 1340955 esxK 886051 esxK Mycobacterium tuberculosis H37Rv ESAT-6 like protein EsxK (ESAT-6 like protein 3) NP_215713.1 1340659 D 83332 CDS NP_215714.1 15608338 886090 1341006..1341290 1 NC_000962.3 Rv1198, (MT1236, MTCI364.10), len: 94 aa. EsxL,ESAT-6 like protein (see citation below). Member of the ESAT-6 family with Rv3619c, Rv1037c, etc. Almost identical to MTCY10G2.12 (94 aa) (97.9% identity in 94 aa overlap) and MTCY98.022c (94 aa) (94.7% identity in 94 aa overlap). Highly similar to Q49946|U1756D Mycobacterium leprae (95 aa), FASTA scores: opt: 403, E(): 1.1e-22, (64.1% identity in 92 aa overlap). seems to belong to the ESAT6 family.; Putative ESAT-6 like protein EsxL (ESAT-6 like protein 4) 1341290 esxL 886090 esxL Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxL (ESAT-6 like protein 4) NP_215714.1 1341006 D 83332 CDS NP_215715.1 15608339 886092 complement(1341358..1342605) 1 NC_000962.3 Rv1199c, (MTCI364.11c), len: 415 aa. Possible transposase for IS1081, identical to TRA1_MYCBO|P35882 transposase for insertion sequence element (415 aa); region identical to MTCY441.35 (100.0% identity in 261 aa overlap); and almost identical to MTCY10G2.02c (415 aa) (99.8% identity in 415 aa overlap). Contains PS01007 Transposases, Mutator family, signature, PS00435 Peroxidases proximal heme-ligand signature.; Possible transposase 1342605 886092 Rv1199c Mycobacterium tuberculosis H37Rv Possible transposase NP_215715.1 1341358 R 83332 CDS NP_215716.1 15608340 886087 1342942..1344219 1 NC_000962.3 Rv1200, (MTCI364.12), len: 425 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS), similar to others e.g. YHJE_ECOLI|P37643 hypothetical metabolite transport protein from Escherichia coli (440 aa), FASTA scores: opt: 1047, E(): 0, (39.1% identity in 427 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. The transcription of this CDS seems to be activated in macrophages (see citation below).; Probable conserved integral membrane transport protein 1344219 886087 Rv1200 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein NP_215716.1 1342942 D 83332 CDS NP_215717.1 15608341 886088 complement(1344216..1345169) 1 NC_000962.3 Rv1201c, (MTCI364.13c), len: 317 aa. dapD,tetrahydrodipicolinate N-succinyltransferase. Highly similar to Q49948|U1756F Mycobacterium leprae (317 aa),FASTA scores: opt: 1776, E(): 0, (84.9% identity in 317 aa overlap), also Q46064 ORF3 protein from corynebacterium glutamicum (316 aa), FASTA scores: opt: 864, E(): 0, (44.1% identity in 311 aa overlap).; Tetrahydrodipicolinate N-succinyltransferase DapD 1345169 dapD 886088 dapD Mycobacterium tuberculosis H37Rv Tetrahydrodipicolinate N-succinyltransferase DapD NP_215717.1 1344216 R 83332 CDS YP_177796.1 57116842 887386 1345260..1346324 1 NC_000962.3 Rv1202, (MTCI364.14), len: 354 aa. Probable dapE,succinyl-diaminopimelate desuccinylase, similar to DAPE_CORGL|Q59284 succinyl-diaminopimelate desuccinylase from Corynebacterium glutamicum (369 aa), FASTA scores: opt: 1301, E(): 0, (55.7% identity in 359 aa overlap),highly similar to Q49949|U1756G (400 aa), FASTA scores: opt: 2045, E(): 0, (87.0% identity in 354 aa overlap).; Probable succinyl-diaminopimelate desuccinylase DapE 1346324 dapE 887386 dapE Mycobacterium tuberculosis H37Rv Probable succinyl-diaminopimelate desuccinylase DapE YP_177796.1 1345260 D 83332 CDS NP_215719.1 15608343 886086 complement(1346321..1346905) 1 NC_000962.3 Rv1203c, (MTCI364.15c), len: 194 aa. Hypothetical unknown protein.; Hypothetical protein 1346905 886086 Rv1203c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215719.1 1346321 R 83332 CDS NP_215720.1 15608344 887552 complement(1346936..1348624) 1 NC_000962.3 Rv1204c, (MTCI364.16c), len: 562 aa. Conserved hypothetical protein, some similarity to Q55103 CHO-ORF2 from streptomyces SP. (642 aa), FASTA scores: opt: 215,E(): 3.6e-06, (26.4% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A.; hypothetical protein 1348624 887552 Rv1204c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215720.1 1346936 R 83332 CDS NP_215721.1 15608345 886075 1348719..1349282 1 NC_000962.3 Rv1205, (MTCI364.17), len: 187 aa. Conserved hypothetical protein, similar to Q49952 cosmid B1756 from Mycobacterium leprae (187 aa), FASTA scores: opt: 865, E(): 0, (72.4% identity in 174 aa overlap), also similar to FAS6_RHOFA|P46378 hypothetical 21.1 kDa protein in fasciation locus (ORF6) (198 aa), FASTA scores: opt: 368,E(): 1.3e-17, (37.4% identity in 174 aa overlap). Some similarity to YJL055W Hypothetical protein in BTN1-PEP8 intergenic region from Saccharomyces cerevisiae and P48636 hypothetical protein in AZU 5'region from Pseudomonas aeruginosa. The transcription of this CDS seems to be activated specifically in host granulomas (see citation below).; hypothetical protein 1349282 886075 Rv1205 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215721.1 1348719 D 83332 CDS NP_215722.1 15608346 887549 1349332..1351125 1 NC_000962.3 Rv1206, (MTCI364.18), len: 597 aa. Probable fadD6,fatty-acid-CoA synthetase, highly similar to several e.g. NP_251583.1|NC_002516 probable very-long-chain acyl-CoA synthetase from Pseudomonas aeruginosa (608 aa); Q60714 mouse fatty acid transport protein fatp (646 aa), FASTA scores: opt:712, E(): 0, (36.8% identity in 600 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Probable fatty-acid-CoA ligase FadD6 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 1351125 fadD6 887549 fadD6 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD6 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_215722.1 1349332 D 83332 CDS NP_215723.1 15608347 887447 1351191..1352147 1 NC_000962.3 Rv1207, (MTCI364.19), len: 318 aa. folP2,Dihydropteroate synthase 2, similar to many e.g. DHPS_ECOLI|P26282 Escherichia coli (282 aa), FASTA scores: opt: 480, E(): 1.9e-22, (34.4% identity in 270 aa overlap). Contains PS00792 dihydropteroate synthase signature 1,PS00793 dihydropteroate synthase signature 2.; Dihydropteroate synthase 2 FolP2 (DHPS 2) (dihydropteroate pyrophosphorylase 2) 1352147 folP2 887447 folP2 Mycobacterium tuberculosis H37Rv Dihydropteroate synthase 2 FolP2 (DHPS 2) (dihydropteroate pyrophosphorylase 2) NP_215723.1 1351191 D 83332 CDS NP_215724.1 15608348 886085 1352144..1353118 1 NC_000962.3 Rv1208, (MTCI364.20), len: 324 aa. Probable gpgS,glucosyl-3-phosphoglycerate synthase (See Empadinhas et al., 2008), similar to Q49955|U1756L Mycobacterium leprae (318 aa), FASTA scores, opt: 1621, E(): 0, (80.5% identity in 318 aa overlap). Belongs to retaining glycosyltransferase family 81.; Probable glucosyl-3-phosphoglycerate synthase GpgS 1353118 gpgS 886085 gpgS Mycobacterium tuberculosis H37Rv Probable glucosyl-3-phosphoglycerate synthase GpgS NP_215724.1 1352144 D 83332 CDS NP_215725.1 15608349 887600 1353157..1353525 1 NC_000962.3 Rv1209, (MTCI364.21), len: 122 aa. Conserved protein, containing a hydrophobic N-terminus. Similar to Q49956|U1756M hypothetical protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 524, E(): 8.9e-29,(78.6% identity in 112 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 1353525 887600 Rv1209 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215725.1 1353157 D 83332 CDS NP_215726.1 15608350 888035 1353522..1354136 1 NC_000962.3 Rv1210, (MTCI364.22), len: 204 aa. Probable tagA,DNA-3-methyladenine glycosidase I (see citation below),similar to several e.g. 3MG1_ECOLI|P05100 DNA-3-methyladenine glycosidase I from Escherichia coli (187 aa), FASTA scores: opt: 530, E(): 1.3e-27, (44.2% identity in 190 aa overlap); similar to Q49957 Mycobacterium leprae cosmid B1756 (192 aa), FASTA scores: opt: 1042, E(): 0, (80.2% identity in 192 aa overlap).; Probable DNA-3-methyladenine glycosylase I TagA (tag I) (3-methyladenine-DNA glycosylase I, constitutive) (DNA-3-methyladenine glycosidase I) 1354136 tagA 888035 tagA Mycobacterium tuberculosis H37Rv Probable DNA-3-methyladenine glycosylase I TagA (tag I) (3-methyladenine-DNA glycosylase I, constitutive) (DNA-3-methyladenine glycosidase I) NP_215726.1 1353522 D 83332 CDS NP_215727.1 15608351 887990 1354243..1354470 1 NC_000962.3 Rv1211, (MTCI364.23), len: 75 aa. Conserved protein,similar to Q49958|U1756N Mycobacterium leprae (75 aa),FASTA scores: opt: 460, E(): 0, (90.7% identity in 75 aa overlap).; hypothetical protein 1354470 887990 Rv1211 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215727.1 1354243 D 83332 CDS NP_215728.1 15608352 887805 complement(1354498..1355661) 1 NC_000962.3 Rv1212c, (MTCI364.24c), len: 387 aa. Putative glgA,glycosyl transferase, highly similar to AJ243803|SCO243803_2 Putative glycosyl transferase from Streptomyces coelicolor (387 aa), FASTA scores: opt: 1344,E(): 0, (54.9% identity in 388 aa overlap). Also similar to MJ1607 probable hexosyltransferase from Methanococcus jannaschii (390 aa), FASTA scores: opt: 445, E(): 7.8e-23,(27.9% identity in 401 aa overlap). The region from aa 267-355 highly similar to Q49959 cosmid B1756 from Mycobacterium leprae (91 aa), FASTA scores, opt: 471, E(): 4.8e-25, (80.9% identity in 89 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein, Rv3032.; Putative glycosyl transferase GlgA 1355661 glgA 887805 glgA Mycobacterium tuberculosis H37Rv Putative glycosyl transferase GlgA NP_215728.1 1354498 R 83332 CDS NP_215729.1 15608353 887933 1355836..1357050 1 NC_000962.3 Rv1213, (MTCI364.25), len: 404 aa. glgC,glucose-1-phosphate adenylyltransferase, similar to many e.g. GLGC_ECOLI|P00584 Escherichia coli (430 aa), FASTA scores: opt: 1075, E(): 0, (40.3% identity in 407 aa overlap); highly similar to Q49961 GLGC from Mycobacterium leprae (419 aa), FASTA scores: opt: 2532, E(): 0, (92.6% identity in 404 aa overlap). Belongs to the bacterial and plants glucose-1-phosphate adenylyltransferase family.; Glucose-1-phosphate adenylyltransferase GlgC (ADP-glucose synthase) (ADP-glucose pyrophosphorylase) 1357050 glgC 887933 glgC Mycobacterium tuberculosis H37Rv Glucose-1-phosphate adenylyltransferase GlgC (ADP-glucose synthase) (ADP-glucose pyrophosphorylase) NP_215729.1 1355836 D 83332 CDS YP_177797.1 57116843 888362 complement(1357293..1357625) 1 NC_000962.3 Rv1214c, (MTCI364.26c), len: 110 aa. PE14, Member of Mycobacterium tuberculosis PE family (see citation below),appears to be frameshifted but sequence appears to be correct. The 5'-end is atypical as first 9 aa appear to be missing.; PE family protein PE14 1357625 PE14 888362 PE14 Mycobacterium tuberculosis H37Rv PE family protein PE14 YP_177797.1 1357293 R 83332 CDS NP_215731.1 15608355 887684 complement(1357759..1359444) 1 NC_000962.3 Rv1215c, (MTCI364.27c), len: 561 aa. Conserved protein, low similarity to Rv1835c|Y0D8_MYCTU|Q50598 hypothetical 69.9 kDa protein cy1a11.08 (628 aa), FASTA scores: opt: 257, E(): 1.3e-09, (34.1% identity in 185 aa overlap).; hypothetical protein 1359444 887684 Rv1215c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215731.1 1357759 R 83332 CDS NP_215732.1 15608356 888125 complement(1359472..1360146) 1 NC_000962.3 Rv1216c, (MTCI364.28c), len: 224 aa. Probable conserved integral membrane protein, C-terminal region similar to Q49963|U1756P from Mycobacterium leprae (134 aa), FASTA scores: opt: 311, E(): 3.3e-15, (52.2% identity in 113 aa overlap).; Probable conserved integral membrane protein 1360146 888125 Rv1216c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_215732.1 1359472 R 83332 CDS NP_215733.1 15608357 888401 complement(1360155..1361801) 1 NC_000962.3 Rv1217c, (MTCI364.29c), len: 548 aa. Probable tetronasin-transport integral membrane ABC transporter (see citation below), similar to many e.g. AL049754|SCH10_12 probable ABC-type transport system membrane-spanning protein from Streptomyces coelicolor (539 aa), FASTA scores: opt: 1309, E(): 0, (40.9% identity in 550 aa overlap); Q54407|X73633 TnrB3 protein from Streptomyces longisporoflavus (337 aa), FASTA scores: opt: 692, E(): 0,(39.5% identity in 324 aa overlap); etc. Also has regions similar to Mycobacterium leprae proteins Q49964|U1756Q (109 aa), FASTA scores: opt: 431, E(): 3.1e-20, (64.8% identity in 105 aa overlap) and Q49965|U1756R (82 aa), FASTA scores: opt:154, E(): 0.0028, (61.0% identity in 41 aa overlap).; Probable tetronasin-transport integral membrane protein ABC transporter 1361801 888401 Rv1217c Mycobacterium tuberculosis H37Rv Probable tetronasin-transport integral membrane protein ABC transporter NP_215733.1 1360155 R 83332 CDS NP_215734.1 15608358 888518 complement(1361798..1362733) 1 NC_000962.3 Rv1218c, (MTCI61.01c), len: 311 aa. Probable tetronasin-transport ATP-binding protein ABC transporter (see citation below), similar to many e.g. Q54406|X73633|TNRB2 TNRB2 protein from Streptomyces longisporoflavus (300 aa), FASTA scores: opt: 1133, E(): 0,(60.8% identity in 291 aa overlap); etc. Also similar to others in Mycobacterium tuberculosis e.g. MTCY19H9.04 (30.0% identity in 297 aa overlap); etc. Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable tetronasin-transport ATP-binding protein ABC transporter 1362733 888518 Rv1218c Mycobacterium tuberculosis H37Rv Probable tetronasin-transport ATP-binding protein ABC transporter NP_215734.1 1361798 R 83332 CDS NP_215735.1 15608359 888582 complement(1362723..1363361) 1 NC_000962.3 Rv1219c, (MTCI61.02c), len: 212 aa. Probable transcriptional regulatory protein, some similarity in N-terminus to YBIH_ECOLI|P41037 hypothetical transcriptional regulator from Escherichia coli (103 aa),FASTA scores: opt: 143, E(): 8.9e-06, (39.7% identity in 63 aa overlap); Helix turn helix motif from aa 28-49.; Probable transcriptional regulatory protein 1363361 888582 Rv1219c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215735.1 1362723 R 83332 CDS NP_215736.1 15608360 888419 complement(1363503..1364150) 1 NC_000962.3 Rv1220c, (MTCI61.03c), len: 215 aa. Possible methyltransferase, some similarity to MDMC_STRMY|Q00719 o-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores; opt: 289, E(): 1.3e-07, (30.0% identity in 203 aa overlap). Also similar to Mycobacterium tuberculosis methyltransferases Rv0187|MTCI28.26 (32.9% identity in 222 aa overlap) and Rv1703c. Start site chosen by homology; other possible start sites exist upstream.; Probable methyltransferase 1364150 888419 Rv1220c Mycobacterium tuberculosis H37Rv Probable methyltransferase NP_215736.1 1363503 R 83332 CDS NP_215737.1 15608361 888751 1364413..1365186 1 NC_000962.3 Rv1221, (MTCI61.04), len: 257 aa. SigE, alternative sigma factor of extracytoplasmic function (ECF) family (see citations below). Similar to many e.g. RPOE_HAEIN|P44790 RNA polymerase sigma-e factor from Haemophilus influenzae (189 aa), FASTA scores: opt: 247, E(): 3.4e-06, (28.5% identity in 186 aa overlap); etc. Also similar to MTCY07D11.03 rpoE from Mycobacterium tuberculosis (35.2% identity in 159 aa overlap). Belongs to the sigma-70 factor family, ECF subfamily. Three promoters and three translational start codons have been detected (See Dona et al., 2008). Fourth transcriptional start point has been identified (See Pang et al., 2007). Note that in Mycobacterium bovis BCG, the sigE gene is transcribed from two promoters, P1 and P2, and that these promoters were expressed at temperatures from 30-50 degrees Celsius.; Alternative RNA polymerase sigma factor SigE 1365186 sigE 888751 sigE Mycobacterium tuberculosis H37Rv Alternative RNA polymerase sigma factor SigE NP_215737.1 1364413 D 83332 CDS NP_215738.1 15608362 885196 1365344..1365808 1 NC_000962.3 Rv1222, (MTCI61.05), len: 154 aa. RseA, anti-sigma factor (See Dona et al., 2008). Identical to O06290|MTU87242 (but shorter due to different start site chosen by proximity of RBS). Equivalent to O05736|U87308|MAU87308_2 hypothetical protein from Mycobacterium avium (133 aa), FASTA scores: opt: 644, E(): 7e-32, (86.2% identity in 109 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Anti-sigma factor RseA 1365808 rseA 885196 rseA Mycobacterium tuberculosis H37Rv Anti-sigma factor RseA NP_215738.1 1365344 D 83332 CDS NP_215739.2 57116844 888912 1365875..1367461 1 NC_000962.3 Rv1223, (MTCI61.06), len: 528 aa. Probable htrA (alternate gene name: degP), serine protease precursor (see citations below), equivalent to U15180|MLU15180_31|Q49972|ML1078|HTRA possible serine protease from Mycobacterium leprae (533 aa), FASTA scores: opt: 2777, E(): 4.1e-141, (81.6% identity in 533 aa overlap). Also similar to many others e.g. HTRA_ECOLI|P09376 protease do precursor from Escherichia coli (474 aa), FASTA scores: opt: 581, E(): 9.1e-27, (36.3% identity in 278 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Start changed since first submission (-21 aa). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable serine protease HtrA (DEGP protein) 1367461 htrA 888912 htrA Mycobacterium tuberculosis H37Rv Probable serine protease HtrA (DEGP protein) NP_215739.2 1365875 D 83332 CDS NP_215740.1 15608364 887143 1367463..1367858 1 NC_000962.3 Rv1224, (MTCI61.07), len: 131 aa. Probable tatB,component of twin-arginine translocation protein export system (see citation below). Possible exported protein with hydrophobic stretch at N-terminus. Highly similar to Q49973|U15180 hypothetical protein U1756Y from Mycobacterium leprae (120 aa), FASTA scores: opt: 601, E(): 0, (73.3% identity in 131 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable protein TatB 1367858 tatB 887143 tatB Mycobacterium tuberculosis H37Rv Probable protein TatB NP_215740.1 1367463 D 83332 CDS NP_215741.1 15608365 887148 complement(1367891..1368721) 1 NC_000962.3 Rv1225c, (MTCI61.08c), len: 276 aa. Conserved hypothetical protein, some similarity to other hypothetical proteins e.g. AE001078|AE001078_2 Archaeoglobus fulgidus (265 aa), FASTA scores: opt: 339, E(): 5.1e-15, (27.1% identity in 262 aa overlap), and to NAGD_ECOLI|P15302 nagd protein from Escherichia coli (250 aa), FASTA scores: opt: 167, E(): 6.4e-12, (24.8% identity in 258 aa overlap). Also weakly similar to Mycobacterium tuberculosis hypothetical protein Rv3400|MTCY78.28c (29.1% identity in 251 aa overlap).; hypothetical protein 1368721 887148 Rv1225c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215741.1 1367891 R 83332 CDS NP_215742.1 15608366 887144 complement(1368832..1370295) 1 NC_000962.3 Rv1226c, (MTCI61.09c), len: 487 aa. Probable transmembrane protein. Some similarity to AL049841|SCE9.01 Streptomyces coelicolor (436 aa), FASTA scores: opt: 203,E(): 1.2e-05, (29.8% identity in 346 aa overlap).; Probable transmembrane protein 1370295 887144 Rv1226c Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_215742.1 1368832 R 83332 CDS NP_215743.1 15608367 885872 complement(1370292..1370825) 1 NC_000962.3 Rv1227c, (MTCI61.10c), len: 177 aa. Possible transmembrane protein, similar to P96615 hypothetical protein ydbS from Bacillus subtilis (159 aa), fasta scores: E(): 3.6e-07, (30.1% identity in 163 aa overlap).; Probable transmembrane protein 1370825 885872 Rv1227c Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_215743.1 1370292 R 83332 CDS NP_215744.1 15608368 886013 1370920..1371477 1 NC_000962.3 Rv1228, (MTCI61.11), len: 185 aa. Probable lipoprotein LpqX. Contains possible signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LpqX 1371477 lpqX 886013 lpqX Mycobacterium tuberculosis H37Rv Probable lipoprotein LpqX NP_215744.1 1370920 D 83332 CDS NP_215745.1 15608369 886067 complement(1371777..1372949) 1 NC_000962.3 Rv1229c, (MT1267, MTCI61.12c, MTV006.01c), len: 390 aa. Probable Mrp protein, similar to others e.g. MRP_ECOLI|P21590 mrp protein from Escherichia coli (379 aa), FASTA scores: E(): 0, (34.1% identity in 355 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS01215 MRP Prosite domain. Belongs to the MRP/NBP35 family of ATP-binding proteins.; Probable Mrp-related protein Mrp 1372949 mrp 886067 mrp Mycobacterium tuberculosis H37Rv Probable Mrp-related protein Mrp NP_215745.1 1371777 R 83332 CDS NP_215746.1 15608370 887134 complement(1372962..1374197) 1 NC_000962.3 Rv1230c, (MTV006.02c), len: 411 aa. Possible membrane protein with two hydrophobic stretches near N-terminus. Some similarity to Rv1022|MTCY10G2.27c|Z92539 probable lpqU protein Mycobacterium tuberculosis (243 aa),FASTA score: opt: 408, E(): 1e-11, (43.6% identity in 172 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA score: opt: 679, E(): 5.1e-23, (36.4% identity in 398 aa overlap).; Possible membrane protein 1374197 887134 Rv1230c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215746.1 1372962 R 83332 CDS NP_215747.1 15608371 887140 complement(1374322..1374864) 1 NC_000962.3 Rv1231c, (MTV006.03c), len: 180 aa. Probable membrane protein, similar to others e.g. AL390975 Streptomyces coelicolor (198 aa).; Probable membrane protein 1374864 887140 Rv1231c Mycobacterium tuberculosis H37Rv Probable membrane protein NP_215747.1 1374322 R 83332 CDS NP_215748.1 15608372 886014 complement(1374861..1376168) 1 NC_000962.3 Rv1232c, (MTV006.04c), len: 435 aa. Conserved protein, similar to other hypothetical proteins e.g. AB013374|AB013374_2 Bacillus halodurans C-125 mamX (449 aa), FASTA scores: opt: 381, E(): 1e-16, (29.9% identity in 251 aa overlap). Some similarity in N-terminus to U15180|MLU1518033 hypothetical Mycobacterium leprae protein u1756u (329 aa), FASTA scores: opt: 300, E(): 4.1e-12,(69.3% identity in 75 aa overlap).; hypothetical protein 1376168 886014 Rv1232c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215748.1 1374861 R 83332 CDS NP_215749.1 15608373 887131 complement(1376230..1376826) 1 NC_000962.3 Rv1233c, (MTV006.05c), len: 198 aa. Conserved hypothetical membrane protein, N-terminus is highly proline rich, C-terminus has two hydrophobic stretches. Proline-rich N-terminus has some similarity to CBPA_DICDI calcium binding protein from Dictyostelium discoideum (467 aa), FASTA scores: E(): 4.8e-06, (35.5% identity in 183 aa overlap). Both sequences share multiple copies of a Tyr-Pro-Pro motif.; hypothetical protein 1376826 887131 Rv1233c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215749.1 1376230 R 83332 CDS NP_215750.1 15608374 887137 1376976..1377503 1 NC_000962.3 Rv1234, (MTV006.06), len: 175 aa. Possible transmembrane protein with two TM helices.; Probable transmembrane protein 1377503 887137 Rv1234 Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_215750.1 1376976 D 83332 CDS NP_215751.1 15608375 887145 1377524..1378930 1 NC_000962.3 Rv1235, (MTV006.07), len: 468 aa. Probable lpqY,sugar-binding lipoprotein component of sugar transport system (see citation below), equivalent to MLU1518034 protein u1756v from Mycobacterium leprae (469 aa), FASTA scores: opt: 2442, E(): 0, (77.4% identity in 470 aa overlap). Also similar to P18815|MALE_ENTAE maltose-binding periplasmic protein from Enterobacter aerogenes (396 aa),FASTA scores: opt: 193, E(): 2.3e-05, (24.2% identity in 297 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable sugar-binding lipoprotein LpqY 1378930 lpqY 887145 lpqY Mycobacterium tuberculosis H37Rv Probable sugar-binding lipoprotein LpqY NP_215751.1 1377524 D 83332 CDS NP_215752.1 15608376 887124 1378927..1379850 1 NC_000962.3 Rv1236, (MTV006.08), len: 307 aa. Probable sugA,sugar-transport integral membrane protein ABC transporter (see citation below), equivalent to U15180|MLU1518035 protein malFM from Mycobacterium leprae (310 aa), FASTA scores: opt: 1566, E(): 0, (81.8% identity in 292 aa overlap). Also similar to numerous bacterial sugar transport system components. Also similar to Rv2316|MTCY3G12.18c from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 514, E(): 7.3e-27, (33.2% identity in 283 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature.; Probable sugar-transport integral membrane protein ABC transporter SugA 1379850 sugA 887124 sugA Mycobacterium tuberculosis H37Rv Probable sugar-transport integral membrane protein ABC transporter SugA NP_215752.1 1378927 D 83332 CDS NP_215753.1 15608377 887121 1379855..1380679 1 NC_000962.3 Rv1237, (MTV006.09), len: 274 aa. Probable sugB,sugar-transport integral membrane protein ABC transporter (see citation below), equivalent to U15180|MLU1518036 protein MalGM from Mycobacterium leprae (296 aa), FASTA scores: opt: 1571, E(): 0, (89.8% identity in 274 aa overlap). Also similar to numerous bacterial sugar transport protein. Related to Rv2834c|MTCY16B7.08 from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 370, E(): 2.4e-17, (26.8% identity in 269 aa overlap).; Probable sugar-transport integral membrane protein ABC transporter SugB 1380679 sugB 887121 sugB Mycobacterium tuberculosis H37Rv Probable sugar-transport integral membrane protein ABC transporter SugB NP_215753.1 1379855 D 83332 CDS NP_215754.1 15608378 887104 1380684..1381865 1 NC_000962.3 Rv1238, (MTV006.10), len: 393 aa. Probable sugC,sugar-transport ATP-binding protein ABC transporter (see citation below). Highly similar to U15180 protein ugpC from Mycobacterium leprae (392 aa), FASTA score: opt: 2007, E(): 0, (79.9% identity in 389 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable sugar-transport ATP-binding protein ABC transporter SugC 1381865 sugC 887104 sugC Mycobacterium tuberculosis H37Rv Probable sugar-transport ATP-binding protein ABC transporter SugC NP_215754.1 1380684 D 83332 CDS NP_215755.1 15608379 887106 complement(1381942..1383042) 1 NC_000962.3 Rv1239c, (MTV006.11c), len: 366 aa. Possible corA,magnesium and cobalt transport transmembrane protein,highly similar to U15180 corA protein from Mycobacterium leprae (373 aa), FASTA scores: opt: 1985, E(): 0, (79.1% identity in 369 aa overlap). Also similar to various CorA proteins of Gram negative bacteria e.g. P27841|CORA_ECOLI|B3816|Z5333|ECS4746 Magnesium and cobalt transport protein from Escherichia coli strains K12 and O157:H7 (316 aa), FASTA scores: opt: 236, E(): 8e-08,(24.5% identity in 306 aa overlap); etc. Seems to belong to the MIT family.; Possible magnesium and cobalt transport transmembrane protein CorA 1383042 corA 887106 corA Mycobacterium tuberculosis H37Rv Possible magnesium and cobalt transport transmembrane protein CorA NP_215755.1 1381942 R 83332 CDS NP_215756.1 15608380 887119 1383213..1384202 1 NC_000962.3 Rv1240, (MTV006.12), len: 329 aa. Probable mdh,Malate dehydrogenase. Most similar to P50917|MDH_MYCLE malate dehydrogenase from Mycobacterium leprae (329 aa),FASTA scores: opt: 1887, E(): 0, (89.1% identity in 329 aa overlap). Contains PS00068 Malate dehydrogenase active site signature. Belongs to the LDH family. MDH subfamily.; Probable malate dehydrogenase Mdh 1384202 mdh 887119 mdh Mycobacterium tuberculosis H37Rv Probable malate dehydrogenase Mdh NP_215756.1 1383213 D 83332 CDS NP_215757.1 15608381 887118 1384278..1384538 1 NC_000962.3 Rv1241, (MTV006.13), len: 86 aa. Possible vapB33,antitoxin, part of toxin-antitoxin (TA) operon with Rv1242,see Arcus et al. 2005. Member of family of 16 hypothetical Mycobacterium tuberculosis proteins including: Rv2871|Q10799|YS71_MYCTU hypothetical 13.2 kDa protein CY2 (124 aa), FASTA scores: opt: 172, E(): 9.5e-06, (37.2% identity in 86 aa overlap); Rv2132, Rv3321c, etc.; Possible antitoxin VapB33 1384538 vapB33 887118 vapB33 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB33 NP_215757.1 1384278 D 83332 CDS NP_215758.1 15608382 887095 1384535..1384966 1 NC_000962.3 Rv1242, (MTV006.14), len: 143 aa. Possible vapC33,toxin, part of toxin-antitoxin (TA) operon with Rv1241,contains PIN domain, see Arcus et al. 2005. Member of family of 14 hypothetical Mycobacterium tuberculosis proteins including: Rv2872|Q10800|YS72_MYCTU (147 aa),FASTA scores: opt: 226, E(): 2.7e-09, (32.1% identity in 137 aa overlap); Rv0749, Rv0277c, Rv2530c, etc.; Possible toxin VapC33 Contains PIN domain 1384966 vapC33 887095 vapC33 Mycobacterium tuberculosis H37Rv Possible toxin VapC33 Contains PIN domain NP_215758.1 1384535 D 83332 CDS YP_177798.1 57116845 887109 complement(1384989..1386677) 1 NC_000962.3 Rv1243c, (MTV006.15c), len: 562 aa. PE_PGRS23,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002).; PE-PGRS family protein PE_PGRS23 1386677 PE_PGRS23 887109 PE_PGRS23 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS23 YP_177798.1 1384989 R 83332 CDS NP_215760.1 15608384 887093 1386857..1387717 1 NC_000962.3 Rv1244, (MTV006.16), len: 286 aa. Probable lipoprotein lpqZ, equivalent toU15180|MLU1518042 protein u1756x from Mycobacterium leprae (228 aa), FASTA scores: opt: 1039, E(): 0, (72.5% identity in 229 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv3759c. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LpqZ 1387717 lpqZ 887093 lpqZ Mycobacterium tuberculosis H37Rv Probable lipoprotein LpqZ NP_215760.1 1386857 D 83332 CDS NP_215761.1 15608385 887091 complement(1387798..1388628) 1 NC_000962.3 Rv1245c, (MTV006.17c), len: 276 aa. Probable short-chain dehydrogenase/reductase, equivalent to NP_301801.1|NC_002677 short chain alcohol dehydrogenase from Mycobacterium leprae (277 aa). Also highly similar to various dehydrogenases and oxidoreductases e.g. NP_250228.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (295 aa); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv3085|MTV013.06 probable short-chain type dehydrogenase/reductase (276 aa),FASTA scores: opt: 368, E(): 1.2e-16, (35.3% identity in 224 aa overlap); Rv3057c|MTCY22D7.24 putative short chain alcohol dehydrogenase/reductase (287 aa), FASTA scores: opt: 471, E(): 1.3e-21, (32.4% identity in 281 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 1388628 887091 Rv1245c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_215761.1 1387798 R 83332 CDS NP_215762.1 15608386 887099 complement(1388685..1388978) 1 NC_000962.3 Rv1246c, (MTV006.18c), len: 97 aa. RelE, toxin, part of toxin-antitoxin (TA) operon with Rv1247c (See Pandey and Gerdes, 2005), highly similar to Rv2866|MTV003.12 hypothetical Mycobacterium tuberculosis protein (87 aa),FASTA scores: opt: 290, E(): 3.9e-24, (54.1% identity in 85 aa overlap).; Toxin RelE 1388978 relE 887099 relE Mycobacterium tuberculosis H37Rv Toxin RelE NP_215762.1 1388685 R 83332 CDS NP_215763.1 15608387 887086 complement(1388975..1389244) 1 NC_000962.3 Rv1247c, (MTV006.19c), len: 89 aa. RelB, antitoxin,part of toxin-antitoxin (TA) operon with Rv1246c (See Pandey and Gerdes, 2005), some similarity to hypothetical proteins including Mycobacterium tuberculosis proteins Rv2865|MTV003.11 (93 aa), FASTA scores: opt: 249, E(): 5.4e-13, (44.2% identity in 86 aa overlap); Rv0268|Z86089|P95225 (169 aa) opt: 125, E(): 0.0089, (41.8% identity in 55 aa overlap); etc. and Escherichia coli AE000293|ECAE0002933 (92 aa), FASTA scores: opt: 127, E(): 0.0038, (29.3% identity in 82 aa overlap).; Antitoxin RelB 1389244 relB 887086 relB Mycobacterium tuberculosis H37Rv Antitoxin RelB NP_215763.1 1388975 R 83332 CDS NP_215764.2 161352467 887084 complement(1389357..1393052) 1 NC_000962.3 Rv1248c, (MTV006.20c), len: 1231 aa. Multifunctional alpha-ketoglutarate metabolic enzyme, highly similar to D84102 Corynebacterium glutamicum (1257 aa), FASTA scores: opt: 4418, E(): 0, (59.4% identity in 1223 aa overlap). Cofactor: thiamine diphosphate. Start changed since first submission (+17 aa).; Multifunctional alpha-ketoglutarate metabolic enzyme 1393052 887084 Rv1248c Mycobacterium tuberculosis H37Rv Multifunctional alpha-ketoglutarate metabolic enzyme NP_215764.2 1389357 R 83332 CDS NP_215765.1 15608389 887077 complement(1393194..1393982) 1 NC_000962.3 Rv1249c, (MTV006.21c), len: 262 aa. Possible membrane protein. Start uncertain. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible membrane protein 1393982 887077 Rv1249c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215765.1 1393194 R 83332 CDS NP_215766.1 15608390 887073 1394179..1395918 1 NC_000962.3 Rv1250, (MTV006.22), len: 579 aa. Probable drug-transport integral membrane protein, member of major facilitator superfamily (MFS), highly similar to several including P39886|TCMA_STRGA tetracenomycin C resistance protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 847, E(): 0, (32.9% identity in 517 aa overlap); etc. Also similar to MTCY20B11.14c|Rv3239C from Mycobacterium tuberculosis (1048 aa), FASTA scores: opt: 629, E(): 6.7e-13, (31.9% identity in 423 aa overlap).; Probable drug-transport integral membrane protein 1395918 887073 Rv1250 Mycobacterium tuberculosis H37Rv Probable drug-transport integral membrane protein NP_215766.1 1394179 D 83332 CDS NP_215767.1 15608391 887080 complement(1395821..1399240) 1 NC_000962.3 Rv1251c, (MTV006.23c), len: 1139 aa. Conserved hypothetical protein, showing some similarity in C-terminal region with other proteins from eukaryotes and bacteria e.g. NP_142121.1 hypothetical protein from Pyrococcus horikoshii (1188 aa); and some similarity to GTP-binding proteins e.g. P23249|MV10_MOUSE putative GTP-binding protein (1004 aa), FASTA scores: opt: 228, E(): 1.7e-06,(27.7% identity in 560 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1399240 887080 Rv1251c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215767.1 1395821 R 83332 CDS NP_215768.1 15608392 887064 complement(1399296..1399904) 1 NC_000962.3 Rv1252c, (MTCY50.30), len: 202 aa. Probable lipoprotein lprE, some similarity to Mycobacterium tuberculosis protein Rv3483c|MTCY13E12.36C (220 aa), FASTA scores: E(): 7e-05, (29.5% identity in 200 aa overlap). Contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable lipoprotein LprE 1399904 lprE 887064 lprE Mycobacterium tuberculosis H37Rv Probable lipoprotein LprE NP_215768.1 1399296 R 83332 CDS NP_215769.1 15608393 887069 1399970..1401661 1 NC_000962.3 Rv1253, (MTCY50.29c), len: 563 aa. Probable deaD,Cold-shock dead-box protein A homolog, similar to many e.g. DEAD_ECOLI|P23304 Escherichia coli (646 aa), FASTA scores: opt: 1490, E(): 0, (46.7% identity in 578 aa overlap); similar to Mycobacterium tuberculosis Rv3211. Contains PS00017 ATP/GTP-binding site motif A, PS00039 dead-box subfamily ATP-dependent helicases signature. Belongs to the dead box family helicase.; Probable cold-shock DeaD-box protein A homolog DeaD (ATP-dependent RNA helicase dead homolog) 1401661 deaD 887069 deaD Mycobacterium tuberculosis H37Rv Probable cold-shock DeaD-box protein A homolog DeaD (ATP-dependent RNA helicase dead homolog) NP_215769.1 1399970 D 83332 CDS NP_215770.1 15608394 887076 1401658..1402809 1 NC_000962.3 Rv1254, (MTCY50.28c), len: 383 aa. Probable Acyltransferase, similar to G927228 midecamycin 4-0-propionyl transferase (fragment) (388 aa), FASTA scores, opt: 305, E(): 5.6e-14, (28.4% identity in 377 aa overlap). Also similar to other Mycobacterium tuberculosis acyltransferases e.g. Rv0111, Rv0228, etc. Contains PS00881 Protein splicing signature.; Probable acyltransferase 1402809 887076 Rv1254 Mycobacterium tuberculosis H37Rv Probable acyltransferase NP_215770.1 1401658 D 83332 CDS NP_215771.1 15608395 887068 complement(1402778..1403386) 1 NC_000962.3 Rv1255c, (MTCY50.27), len: 202 aa. Possible regulatory protein, similar to others e.g. ACRR_ECOLI|P34000 potential acrab operon repressor from E. coli (215 aa), FASTA scores: opt: 128, E(): 0.25, (42.1% identity in 57 aa overlap). Helix turn helix motif present at aa 36-57 (+5.48 SD).; Probable transcriptional regulatory protein 1403386 887068 Rv1255c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215771.1 1402778 R 83332 CDS NP_215772.1 15608396 887059 complement(1403386..1404603) 1 NC_000962.3 Rv1256c, (MT1295, MTCY50.26), len: 405 aa. Probable cyp130, cytochrome P450, similar to other cytochromes P-450 e.g. S51594 cytochrome P450 mycG from Micromonospora griseorubida (397 aa); T36526 probable cytochrome P450 hydroxylase from Streptomyces coelicolor (411 aa); CPXK_SACER|P33271|107B1 cytochrome P450 from Saccharopolyspora erythraea (405 aa), FASTA scores: opt: 639, E(): 2.7e-33, (33.2% identity in 391 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0766c|MTCY369.11c cytochrome P450 (402 aa); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Probable cytochrome P450 130 Cyp130 1404603 cyp130 887059 cyp130 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 130 Cyp130 NP_215772.1 1403386 R 83332 CDS NP_215773.1 15608397 887063 complement(1404717..1406084) 1 NC_000962.3 Rv1257c, (MTCY50.25), len: 455 aa. Probable oxidoreductase, similar to e.g. GLCD_ECOLI|P52075 glycolate oxidase subunit glcd (499 aa), FASTA scores: E(): 0, (38.9% identity in 458 aa overlap). Similar to Mycobacterium tuberculosis oxidoreductases e.g. Rv3107c; Probable oxidoreductase 1406084 887063 Rv1257c Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_215773.1 1404717 R 83332 CDS NP_215774.1 15608398 887056 complement(1406081..1407340) 1 NC_000962.3 Rv1258c, MTCY50.24, len: 419 aa. Probable conserved integral membrane transport (efflux) protein, possibly member of major facilitator superfamily (MFS), highly similar to O32859|tap protein multidrug-resistance efflux pump from Mycobacterium fortuitum (409 aa), FASTA scores: E(): 0, (68.4% identity in 408 aa overlap). Contains PS00216 Sugar transport proteins signature 1.; Probable conserved integral membrane transport protein 1407340 887056 Rv1258c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein NP_215774.1 1406081 R 83332 CDS NP_215775.1 15608399 887047 1407339..1408238 1 NC_000962.3 Rv1259, (MTCY50.23c), len: 299 aa. Probable udgB,uracil DNA glycosylase. Similar to AL109732|SC7H2.04 hypothetical protein from Streptomyces coelicolor (237 aa),FASTA scores: opt: 870, E(): 0, (57.1% identity in 231 aa overlap).; Probable uracil DNA glycosylase, UdgB 1408238 udgB 887047 udgB Mycobacterium tuberculosis H37Rv Probable uracil DNA glycosylase, UdgB NP_215775.1 1407339 D 83332 CDS NP_215776.1 15608400 887044 1408240..1409358 1 NC_000962.3 Rv1260, (MTCY50.22c), len: 372 aa. Probable oxidoreductase, highly similar to E1245747|AL021411 putative oxidoreductase SC7H1.18 from Streptomyces coelicolor (397 aa), FASTA scores: E(): 1.4e-29, (45.9% identity in 355 aa overlap); also some similarity to G912582 FAD binding protein homologue from Pseudomonas aeruginosa (286 aa), FASTA scores: opt: 245, E(): 2e-09,(27.5% identity in 251 aa overlap); PCPB_FLASP|P42535 pentachlorophenol 4-monooxygenase (537 aa), FASTA scores: opt: 219, E(): 1.7e-07, (23.3% identity in 360 aa overlap); TETX_BACFR|Q01911 tetracycline resistance protein (388 aa),FASTA scores: opt: 183, E(): 3e-05, (22.8% identity in 373 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0575c and Rv1751.; Probable oxidoreductase 1409358 887044 Rv1260 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_215776.1 1408240 D 83332 CDS NP_215777.1 15608401 887055 complement(1409484..1409933) 1 NC_000962.3 Rv1261c, (MTCY50.21), len: 149 aa. Conserved protein, similar to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1558|MTCY48.07c (39.2% identity in 125 aa overlap); Rv3547 and Rv3178.; hypothetical protein 1409933 887055 Rv1261c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215777.1 1409484 R 83332 CDS NP_215778.1 15608402 887036 complement(1409938..1410372) 1 NC_000962.3 Rv1262c, (MTCY50.20), len: 144 aa. Hypothetical hit-like protein, similar to Q04344|HIT_YEAST hit1 protein (orf u) (144 aa), FASTA scores: opt: 306, E(): 3e-14, (35.9 % identity in 142 aa overlap); also similar to YHIT_MYCGE|P47378 hypothetical 15.6 kDa protein (141 aa),FASTA scores: opt: 250, E(): 1.6e-10, (35.5% identity in 107 aa overlap); and YHIT_MYCLE|P49774 hypothetical 17.0 kDa protein hit-like (155 aa), FASTA scores: opt: 196, E(): 7e-07, (30.6% identity in 144 aa overlap). Similar to other proteins from Mycobacterium tuberculosis e.g. Rv2613c,Rv0759c. Contains PS00892 hit family signature. Belongs to the hit family.; Hypothetical hit-like protein 1410372 887036 Rv1262c Mycobacterium tuberculosis H37Rv Hypothetical hit-like protein NP_215778.1 1409938 R 83332 CDS NP_215779.1 15608403 887041 1410431..1411819 1 NC_000962.3 Rv1263, (MTCY50.19c), len: 462 aa. Probable amiB2,amidase. Similar to G1001278 hypothetical 54.3 kDa protein (506 aa), FASTA scores: opt: 767, E(): 7.6e-40, (32.8% identity in 461 aa overlap), also similar to G580673 rhodococcus enantiose lective amidase gene (462 aa), FASTA scores, opt: 668, E(): 7.4e-34, (33.5% identity in 484 aa overlap) also to NYLA_PSES8|P13398 6-aminohexanoate-cyclic-dimer hydrolase (492 aa), FASTA scores opt: 543, E(): 3.1e-26, (33.5% identity in 493 aa overlap). Also similar to MTCY274.19c (33.5% identity in 427 aa overlap). Similar to other putative amidases in M. tuberculosis; Rv2363, Rv2888c, etc. Contains PS00017 ATP/GTP-binding site motif A. Belongs to the amidase family.; Probable amidase AmiB2 (aminohydrolase) 1411819 amiB2 887041 amiB2 Mycobacterium tuberculosis H37Rv Probable amidase AmiB2 (aminohydrolase) NP_215779.1 1410431 D 83332 CDS NP_215780.1 15608404 887035 1411894..1413087 1 NC_000962.3 Rv1264, (MTCY50.18c), len: 397 aa. Adenylate cyclase (function proven experimentally: see Linder et al., 2002),showing some similarity to other adenylate cyclases e.g. CYAA_BRELI|P27580 (403 aa), FASTA scores, opt: 270, E(): 1.3e-10, (29.3% identity in 317 aa overlap); etc. Similar to other putative cyclases in M. tuberculosis e.g. Rv2212,Rv1647. The C terminus seems to code for a catalytic domain belonging to a subfamily of adenylyl cyclase isozymes (mostly found in Gram-positive bacteria). The N terminus seems to be a potential novel regulator of adenylyl cyclase activity (autoinhibitory domain). Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.; Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) 1413087 887035 Rv1264 Mycobacterium tuberculosis H37Rv Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) NP_215780.1 1411894 D 83332 CDS NP_215781.1 15608405 887058 1413260..1413940 1 NC_000962.3 Rv1265, (MTCY50.17c), len: 226 aa. Unknown protein (see citation below).; hypothetical protein 1413940 887058 Rv1265 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215781.1 1413260 D 83332 CDS NP_215782.1 15608406 887023 complement(1413960..1415840) 1 NC_000962.3 Rv1266c, (MTCY50.16), len: 626 aa. Probable pknH,transmembrane serine/threonine-protein kinase (see citation below), similar to many e.g. PKN1_MYXXA|P33973 pkn1 (693 aa), FASTA scores: opt: 611, E(): 1.4e- 14, (29.7% identity in 492 aa overlap); etc. Contains PS00107 Protein kinases ATP-binding region signature; PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation.; Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H) 1415840 pknH 887023 pknH Mycobacterium tuberculosis H37Rv Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H) NP_215782.1 1413960 R 83332 CDS NP_215783.1 15608407 887026 complement(1416181..1417347) 1 NC_000962.3 Rv1267c, (MT1305, MTCY50.15), len: 388 aa. Probable embR, regulatory protein (see citation below), similar to many e.g. AFSR_STRCO|P25941 regulatory protein AfsR from Streptomyces coelicolor (993 aa), FASTA scores: opt: 489,E(): 1e-25, (33.5% identity in 361 aa overlap); etc. Belongs to the AFSR/DNRI/REDD family of regulators. Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al.,2010).; Probable transcriptional regulatory protein EmbR 1417347 embR 887026 embR Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein EmbR NP_215783.1 1416181 R 83332 CDS NP_215784.1 15608408 887033 complement(1417658..1418356) 1 NC_000962.3 Rv1268c, (MTCY50.14), len: 232 aa. Hypothetical unknown protein, probably secreted protein : contains possible signal peptide sequence (score 7.9 at residue 28). Predicted to be an outer membrane protein (See Song et al.,2008).; Hypothetical protein 1418356 887033 Rv1268c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215784.1 1417658 R 83332 CDS NP_215785.1 15608409 887039 complement(1418579..1418953) 1 NC_000962.3 Rv1269c, (MTCY50.13), len: 124 aa. Conserved probable exported protein with putative N-terminal signal sequence. Similar to Mycobacterium tuberculosis protein Rv1813c|Y0DU_MYCTU|Q50620 hypothetical protein cy1a11.30 (137 aa), FASTA scores: E(): 9e-21, (41.6% identity in 137 aa overlap).; Conserved probable secreted protein 1418953 887039 Rv1269c Mycobacterium tuberculosis H37Rv Conserved probable secreted protein NP_215785.1 1418579 R 83332 CDS NP_215786.1 15608410 887017 complement(1419014..1419748) 1 NC_000962.3 Rv1270c, (MTCY50.12), len: 244 aa. Possible lprA,lipoprotein. Similar to O32852|AJ000500 lipoprotein from Mycobacterium bovis (236 aa), fasta scores: E(): 5.2e-23,(35.1% identity in 245 aa overlap). Similar to M. tuberculosis lipoproteins: Rv1368, Rv1411c, Rv2945c. Contains probable N-terminal signal sequence.; Possible lipoprotein LprA 1419748 lprA 887017 lprA Mycobacterium tuberculosis H37Rv Possible lipoprotein LprA NP_215786.1 1419014 R 83332 CDS NP_215787.1 15608411 887019 complement(1419961..1420302) 1 NC_000962.3 Rv1271c, (MTCY50.11), len: 113 aa. Conserved hypothetical exported protein with potential N-terminal signal sequence. Similar to Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, Rv0622, etc.; Conserved hypothetical secreted protein 1420302 887019 Rv1271c Mycobacterium tuberculosis H37Rv Conserved hypothetical secreted protein NP_215787.1 1419961 R 83332 CDS NP_215788.1 15608412 887021 complement(1420410..1422305) 1 NC_000962.3 Rv1272c, (MTCY50.10), len: 631 aa. Probable drugs-transport transmembrane ATP-binding protein ABC transporter (see citation below), similar to e.g. Y015_MYCGE|P47261 hypothetical ABC transporter mg015m from Mycoplasma genitalium (589 aa), FASTA scores: opt: 1054,E(): 0, (34.3% identity in 522 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters),MSBA subfamily.; Probable drugs-transport transmembrane ATP-binding protein ABC transporter 1422305 887021 Rv1272c Mycobacterium tuberculosis H37Rv Probable drugs-transport transmembrane ATP-binding protein ABC transporter NP_215788.1 1420410 R 83332 CDS NP_215789.1 15608413 887025 complement(1422302..1424050) 1 NC_000962.3 Rv1273c, (MTCY50.09), len: 582 aa. Probable drugs-transport transmembrane ATP-binding protein ABC transporter (see citation below), similar to e.g. YWJA_BACSU|P45861 hypothetical abc transporter from B. subtilis (575 aa), FASTA scores: opt: 810, E(): 0, (27.0% identity in 578 aa overlap); etc. Contains PS00136 Serine proteases, subtilase family, aspartic acid active site; 2 x PS00211 ABC transporters family signature; and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters),MSBA subfamily.; Probable drugs-transport transmembrane ATP-binding protein ABC transporter 1424050 887025 Rv1273c Mycobacterium tuberculosis H37Rv Probable drugs-transport transmembrane ATP-binding protein ABC transporter NP_215789.1 1422302 R 83332 CDS NP_215790.1 15608414 887011 1424197..1424754 1 NC_000962.3 Rv1274, (MTCY50.08c), len: 185 aa. Possible lprB,lipoprotein; contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Some similarity to Rv1275. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible lipoprotein LprB 1424754 lprB 887011 lprB Mycobacterium tuberculosis H37Rv Possible lipoprotein LprB NP_215790.1 1424197 D 83332 CDS NP_215791.1 15608415 887014 1424751..1425293 1 NC_000962.3 Rv1275, (MTCY50.07c), len: 180 aa. Possible lprC,lipoprotein; contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Some similarity to Rv1274. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible lipoprotein LprC 1425293 lprC 887014 lprC Mycobacterium tuberculosis H37Rv Possible lipoprotein LprC NP_215791.1 1424751 D 83332 CDS NP_215792.1 15608416 887000 complement(1425438..1425914) 1 NC_000962.3 Rv1276c, (MTCY50.06), len: 158 aa. Conserved hypothetical protein, similar to AL096844|SCI28.03 hypothetical protein from Streptomyces coelicolor (172 aa),FASTA scores: opt: 385, E(): 3.3e-19, (43.5% identity in 161 aa overlap). Some similarity to P76502|SIXA_ECOLI phosphohistidine phosphatase SIXA (161 aa), FASTA scores: opt: 146, E(): 0.0047, (31.9% identity in 116 aa overlap). Belongs to the SixA family of phosphatases.; hypothetical protein 1425914 887000 Rv1276c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215792.1 1425438 R 83332 CDS NP_215793.1 15608417 887001 1426164..1427417 1 NC_000962.3 Rv1277, (MTCY50.05c), len: 417 aa. Conserved hypothetical protein, some similarity to 3914967|O68033|SBCD_RHOCA exonuclease SBCD homolog from Rhodobacter capsulatus (405 aa). May be sbcD protein (see Mizrahi & Andersen 1998); hypothetical protein 1427417 887001 Rv1277 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215793.1 1426164 D 83332 CDS NP_215794.1 15608418 887005 1427414..1430041 1 NC_000962.3 Rv1278, (MTCY50.04c), len: 875 aa. Hypothetical unknown protein, possible coiled-coil regions, contains PS00017 ATP/GTP-binding site motif A. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Hypothetical protein 1430041 887005 Rv1278 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215794.1 1427414 D 83332 CDS NP_215795.1 15608419 887002 1430062..1431648 1 NC_000962.3 Rv1279, (MTCY50.03c), len: 528 aa. Probable dehydrogenase, FAD flavoprotein GMC oxidoreductase, similar to several e.g. dBETA_ECOLI|P17444 choline dehydrogenase from Escherichia coli (556 aa), FASTA scores, opt: 1047,E(): 0, (37.7% identity in 541 aa overlap). Similar to Rv0697 putative Mycobacterium tuberculosis GMC oxidoreductase. Contains PS00623 GMC oxidoreductases signature 1, and PS00624 GMC oxidoreductases signature 2. Belongs to the GMC oxidoreductases family.; Probable dehydrogenase FAD flavoprotein GMC oxidoreductase 1431648 887002 Rv1279 Mycobacterium tuberculosis H37Rv Probable dehydrogenase FAD flavoprotein GMC oxidoreductase NP_215795.1 1430062 D 83332 CDS NP_215796.1 15608420 886985 complement(1431665..1433440) 1 NC_000962.3 Rv1280c, (MTCY50.02), len: 591 aa. Probable oppA,oligopeptide-binding lipoprotein component of peptide transport system (see citation below), sharing some similarity to other periplasmic solute binding proteins e.g. OPPA_SALTY|P06202 periplasmic oligopeptide-binding protein from Salmonella typhimurium (542 aa), FASTA scores: E(): 5.1e-05, (22.1% identity in 458 aa overlap); etc. Also similar to Rv1166 and Rv2585c from Mycobacterium tuberculosis. Has possible N-terminal signal sequence and prokaryotic lipoprotein lipid attachment site (PS00013). Belongs to the bacterial extracellular solute-binding protein family 5.; Probable periplasmic oligopeptide-binding lipoprotein OppA 1433440 oppA 886985 oppA Mycobacterium tuberculosis H37Rv Probable periplasmic oligopeptide-binding lipoprotein OppA NP_215796.1 1431665 R 83332 CDS NP_215797.1 15608421 886997 complement(1433433..1435271) 1 NC_000962.3 Rv1281c, (MTCY50.01), len: 612 aa. Probable oppD,oligopeptide-transport ATP-binding protein ABC transporter (see citation below), similar to others e.g. DPPD_BACSU|P26905 dipeptide transport ATP-binding protein from Bacillus subtilis (335 aa), FASTA scores: opt: 983,E(): 0, (48.6% identity in 319 aa overlap); etc. Contains 2 x PS00017 ATP/GTP-binding site motif A (P-loop); 2 x PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable oligopeptide-transport ATP-binding protein ABC transporter OppD 1435271 oppD 886997 oppD Mycobacterium tuberculosis H37Rv Probable oligopeptide-transport ATP-binding protein ABC transporter OppD NP_215797.1 1433433 R 83332 CDS NP_215798.1 15608422 886980 complement(1435268..1436143) 1 NC_000962.3 Rv1282c, (MTCY373.01c-MTCY3H3.01), len: 291 aa. Probable oppC, oligopeptide-transport integral membrane protein ABC transporter (see Braibant et al., 2000),similar to other integral membrane proteins e.g. OPPC_ECOLI|P77664 oligopeptide transport system permease from Escherichia coli (302 aa), FASTA scores: E(): 4.6e-33,(40.7% identity in 275 aa overlap); etc. Also similar to Rv3664c|DPPC probable peptide-transport integral membrane protein from Mycobacterium tuberculosis.; Probable oligopeptide-transport integral membrane protein ABC transporter OppC 1436143 oppC 886980 oppC Mycobacterium tuberculosis H37Rv Probable oligopeptide-transport integral membrane protein ABC transporter OppC NP_215798.1 1435268 R 83332 CDS NP_215799.1 15608423 886981 complement(1436140..1437117) 1 NC_000962.3 Rv1283c, (MTCY373.02c), len: 325 aa. Probable oppB,oligopeptide-transport integral membrane protein ABC transporter (see citation below), similar to other integral membrane proteins e.g. DPPB_ECOLI|P37316 dipeptide transport system permease protein from Escherichia coli (339 aa), FASTA scores: opt: 402, E(): 3.4e-20, (31.0% identity in 345 aa overlap); etc. Also similar to Rv3665c|DppB probable peptide-transport integral membrane protein from Mycobacterium tuberculosis. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature.; Probable oligopeptide-transport integral membrane protein ABC transporter OppB 1437117 oppB 886981 oppB Mycobacterium tuberculosis H37Rv Probable oligopeptide-transport integral membrane protein ABC transporter OppB NP_215799.1 1436140 R 83332 CDS NP_215800.1 15608424 886987 1437324..1437815 1 NC_000962.3 Rv1284, (MTCY373.03), len: 163 aa. CanA,Beta-carbonic anhydrase, proven biochemically (See Suarez Covarrubias et al. 2005) similar to others e.g. AL109663|SC4A10.26 hypothetical protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 567, E(): 1.5e-32,(53.4% identity in 163 aa overla); shows some similarity to hypothetical protein from Methanobacterium thermoautotrophicum. Weak similarity to carbonic anhydrases e.g. U51624|MTU516242|P17582 Methanothermobacter thermautotrophicus (171 aa), FASTA score: opt: 305, E(): 1.2e-14, (35.2% identity in 165 aa overlap).; Beta-carbonic anhydrase 1437815 canA 886987 canA Mycobacterium tuberculosis H37Rv Beta-carbonic anhydrase NP_215800.1 1437324 D 83332 CDS NP_215801.1 15608425 886979 1437909..1438907 1 NC_000962.3 Rv1285, (MTCY373.04), len: 332 aa. Probable cysD,sulfate adenylyltransferase subunit 2 (see Wooff et al.,2002), homology suggests start site at aa 24 or 28, similar to e.g. CYSD_ECOLI|P21156 sulfate adenylate transferase subunit 2 from Escherichia coli (302 aa), FASTA score: opt: 973, E():0, (52.5% identity in 303 aa overlap). Also similar to Mycobacterium tuberculosis Rv2392,3'-phosphoadenylylsulfate reductase. Belongs to the PAPS reductase family. CYSD subfamily. Thought to be differentially expressed within host cells (see Triccas et al., 1999).; Probable sulfate adenylyltransferase subunit 2 CysD 1438907 cysD 886979 cysD Mycobacterium tuberculosis H37Rv Probable sulfate adenylyltransferase subunit 2 CysD NP_215801.1 1437909 D 83332 CDS NP_215802.1 15608426 886978 1438907..1440751 1 NC_000962.3 Rv1286, (MTCY373.05), len: 614 aa. Probable cysN/cysC bifunctional enzyme, sulfate adenylyltransferase subunit 1 and Adenylylsulfate kinase (see Wooff et al.,2002), similar to CYSN_ECOLI|P23845 sulfate adenylate transferase subunit 1 from Escherichia coli (475 aa), FASTA scores: opt: 1291, E():0, (50.2% identity in 428 aa overlap). Contains 2 x PS00017 ATP/GTP-binding site motif A, PS00301 GTP-binding elongation factors signature.; Probable bifunctional enzyme CysN/CysC: sulfate adenyltransferase (subunit 1) + adenylylsulfate kinase 1440751 cysN 886978 cysN Mycobacterium tuberculosis H37Rv Probable bifunctional enzyme CysN/CysC: sulfate adenyltransferase (subunit 1) + adenylylsulfate kinase NP_215802.1 1438907 D 83332 CDS NP_215803.1 15608427 886998 1440805..1441290 1 NC_000962.3 Rv1287, (MTCY373.06), len: 161 aa. Conserved hypothetical protein, similar to VjeB family of proteins e.g. FASTA score: P44675|Y379_HAEIN hypothetical protein HI0379 (150 aa), FASTA scores: opt: 213, E(): 2.5e-08,(30.0% identity in 130 aa overlap) and YJEB_ECOLI|P21498 hypothetical 15.6 kDa protein in pura-vacb (141 aa), opt: 167, E(): 9.5e-06, (25.0% identity in 136 aa overlap). Belongs to the UPF0074 (RFF2) family.; hypothetical protein 1441290 886998 Rv1287 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215803.1 1440805 D 83332 CDS NP_215804.1 15608428 886974 1441348..1442718 1 NC_000962.3 Rv1288, (MTCY373.07), len: 456 aa. Conserved protein, some similarity to A85B_MYCTU|P31952 antigen 85-b precursor (85b) (325 aa), FASTA scores: opt: 199, E(): 2.7e-06, (24.7% identity in 279 aa overlap). Also similar to Q01377|CSP1_CORGL PS1 protein precursor (related to antigen 85 complex) from Corynebacterium glutamicum (657 aa), FASTA scores: opt: 280, E(): 1.9e-10, (26.4% identity in 352 aa overlap). Seems to contain 3 LYSM repeats; hypothetical protein 1442718 886974 Rv1288 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215804.1 1441348 D 83332 CDS NP_215805.1 15608429 886994 1442767..1443399 1 NC_000962.3 Rv1289, (MTCY373.08), len: 210 aa. Unknown protein.; hypothetical protein 1443399 886994 Rv1289 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215805.1 1442767 D 83332 CDS NP_215806.1 15608430 886971 complement(1443482..1445047) 1 NC_000962.3 Rv1290c, (MTCY373.09c), len: 521 aa. Conserved protein (see citation below), similar to AL031013|SC8A6.09 hypothetical protein from Streptomyces coelicolor (443 aa),FASTA scores: opt: 371, E(): 9.5e-17, (28.3% identity in 446 aa overlap).; hypothetical protein 1445047 886971 Rv1290c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215806.1 1443482 R 83332 CDS YP_177642.1 57116846 3205114 1445058..1445372 1 NC_000962.3 Rv1290A, len: 104 aa. Hypothetical unknown protein,equivalent to AAK45590 from Mycobacterium tuberculosis strain CDC1551 (122 aa) but shorter 18 aa.; Hypothetical protein 1445372 3205114 Rv1290A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177642.1 1445058 D 83332 CDS NP_215807.1 15608431 886975 complement(1445499..1445834) 1 NC_000962.3 Rv1291c, (MTCY373.10c), len: 111 aa. Conserved hypothetical secreted protein, similar to others in Mycobacterium tuberculosis e.g. Rv1271c|Q11048|YC71_MYCTU hypothetical 11.6 kDa protein (113 aa), FASTA score: opt: 246, E(): 1.7e-09, (40.0% identity in 110 aa overlap); Rv1804c, Rv1810, Rv0622, etc.; Conserved hypothetical secreted protein 1445834 886975 Rv1291c Mycobacterium tuberculosis H37Rv Conserved hypothetical secreted protein NP_215807.1 1445499 R 83332 CDS NP_215808.1 15608432 886964 1446379..1448031 1 NC_000962.3 Rv1292, (MTCY373.12), len: 550 aa. Probable argS,Arginyl-tRNA synthetase, highly similar to SYR_MYCLE|P45840 Mycobacterium leprae (550 aa), FASTA scores: opt: 3115,E(): 0, (84.9% identity in 550 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family.; Probable arginyl-tRNA synthetase ArgS (ARGRS) (arginine--tRNA ligase) 1448031 argS 886964 argS Mycobacterium tuberculosis H37Rv Probable arginyl-tRNA synthetase ArgS (ARGRS) (arginine--tRNA ligase) NP_215808.1 1446379 D 83332 CDS NP_215809.1 15608433 886960 1448028..1449371 1 NC_000962.3 Rv1293, (MTCY373.13), len: 447 aa. lysA,diaminopimelate decarboxylase (see citation below), almost identical to DCDA_MYCTU|P31848. Contains PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site, PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2. Belongs to family 2 of ornithine, DAP, and arginine decarboxylases.; Diaminopimelate decarboxylase LysA (DAP decarboxylase) 1449371 lysA 886960 lysA Mycobacterium tuberculosis H37Rv Diaminopimelate decarboxylase LysA (DAP decarboxylase) NP_215809.1 1448028 D 83332 CDS NP_215810.1 15608434 886962 1449375..1450700 1 NC_000962.3 Rv1294, (MTCY373.14), len: 441 aa. Probable thrA (hom), homoserine dehydrogenase, highly similar to DHOM_MYCLE|P46806 from Mycobacterium leprae (441 aa), FASTA scores: opt: 2437, E():0, (89.5% identity in 438 aa overlap). Contains PS00017 ATP/GTP-binding site motif A; PS01042 Homoserine dehydrogenase signature. Belongs to the homoserine dehydrogenase family.; Probable homoserine dehydrogenase ThrA 1450700 thrA 886962 thrA Mycobacterium tuberculosis H37Rv Probable homoserine dehydrogenase ThrA NP_215810.1 1449375 D 83332 CDS NP_215811.1 15608435 886957 1450697..1451779 1 NC_000962.3 Rv1295, (MTCY373.15), len: 360 aa. thrC, threonine synthase (see Parish et al., 1999), highly similar to THRC_MYCLE|P45837 Mycobacterium leprae (360 aa), FASTA scores: opt: 2202, E(): 0, (93.9% identity in 359 aa overlap). Contains PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site.; Threonine synthase ThrC (ts) 1451779 thrC 886957 thrC Mycobacterium tuberculosis H37Rv Threonine synthase ThrC (ts) NP_215811.1 1450697 D 83332 CDS NP_215812.1 15608436 886958 1451997..1452947 1 NC_000962.3 Rv1296, (MTCY373.16), len: 316 aa. Probable thrB,homoserine kinase (see citation below), highly similar to KHSE_MYCLE|P45836 from Mycobacterium leprae (314 aa), FASTA scores, opt: 1657, E(): 0, (82.0% identity in 311 aa overlap). Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site, and PS00627 GHMP kinases putative ATP-binding domain.; Probable homoserine kinase ThrB 1452947 thrB 886958 thrB Mycobacterium tuberculosis H37Rv Probable homoserine kinase ThrB NP_215812.1 1451997 D 83332 CDS NP_215813.1 15608437 886952 1453204..1455012 1 NC_000962.3 Rv1297, (MTCY373.17), len: 602 aa. Probable rho,transcription termination factor homolog, highly similar to many e.g. RHO_MYCLE|P45835 Mycobacterium leprae (610 aa),FASTA scores: (81.5% identity in 612 aa overlap). Contains 1 RNA recognition motif (RRM). Nucleotide position 1453608 in the genome sequence has been corrected, T:C resulting in G135G.; Probable transcription termination factor Rho homolog 1455012 rho 886952 rho Mycobacterium tuberculosis H37Rv Probable transcription termination factor Rho homolog NP_215813.1 1453204 D 83332 CDS NP_215814.1 15608438 886955 1455163..1455405 1 NC_000962.3 Rv1298, (MTCY373.18), len: 80 aa. rpmE, 50s ribosomal protein L31, highly similar to many e.g. RL31_MYCLE|P45834 50s ribosomal protein L31 from Mycobacterium leprae (84 aa), FASTA scores: opt: 490, E(): 5.5e-28, (89.6% identity in 77 aa overlap). Contains PS01143 Ribosomal protein L31 signature. Belongs to the L31P family of ribosomal proteins.; 50S ribosomal protein L31 RpmE 1455405 rpmE 886955 rpmE Mycobacterium tuberculosis H37Rv 50S ribosomal protein L31 RpmE NP_215814.1 1455163 D 83332 CDS NP_215815.1 15608439 886948 1455495..1456568 1 NC_000962.3 Rv1299, (MTCY373.19), len: 357 aa. Probable prfA,peptide chain release factor 1 (rf-1), highly similar to many e.g. RF1_MYCLE|P45833 peptide chain release factor 1 (rf-1) from Mycobacterium leprae (357 aa), FASTA scores: opt: 2047, E(): 0, (89.3% identity in 356 aa overlap); also similar to Mycobacterium tuberculosis Rv3105c, prfB peptide chain release factor 2. Contains PS00745 Prokaryotic-type class I peptide chain release factors signature. Belongs to the prokaryotic and mitochondrial release factors family.; Probable peptide chain release factor 1 PrfA (RF-1) 1456568 prfA 886948 prfA Mycobacterium tuberculosis H37Rv Probable peptide chain release factor 1 PrfA (RF-1) NP_215815.1 1455495 D 83332 CDS NP_215816.1 15608440 886950 1456565..1457542 1 NC_000962.3 Rv1300, (MTCY373.20), len: 325 aa. Probable hemK protein homolog, homology suggests translation may start at aa 22, highly similar to many e.g. HEMK_MYCLE|P45832 Mycobacterium leprae (288 aa), FASTA scores: opt: 936, E(): 0, (76.7% identity in 189 aa overlap). Belongs to the HemK family of modification methylases.; Probable HemK protein homolog HemK 1457542 hemK 886950 hemK Mycobacterium tuberculosis H37Rv Probable HemK protein homolog HemK NP_215816.1 1456565 D 83332 CDS NP_215817.1 15608441 886970 1457558..1458211 1 NC_000962.3 Rv1301, (MTCY373.21), len: 217 aa. Conserved protein, highly similar to YRFE_MYCLE|P45831 hypothetical 22.7 kDa protein in rfe-hemk intergenic region, (220 aa),FASTA scores: opt: 1168, E(): 0, (82.8% identity in 215 aa overlap). Contains PS01147 Hypothetical SUA5/yciO/yrdC family signature. Belongs to the SUA5/YRDC/YCIO/YWLC family.; hypothetical protein 1458211 886970 Rv1301 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215817.1 1457558 D 83332 CDS NP_215818.1 15608442 886947 1458295..1459509 1 NC_000962.3 Rv1302, (MTCY373.22), len: 404 aa. Probable rfe (alternate gene name: wecA), undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase (see citation below),equivalent to RFE_MYCLE|P45830 Mycobacterium leprae (398 aa), FASTA scores, opt: 2285, E(): 0, (89.2% identity in 398 aa overlap).; Probable undecapaprenyl-phosphate alpha-N-acetylglucosaminyltransferase Rfe (UDP-GlcNAc transferase) 1459509 rfe 886947 rfe Mycobacterium tuberculosis H37Rv Probable undecapaprenyl-phosphate alpha-N-acetylglucosaminyltransferase Rfe (UDP-GlcNAc transferase) NP_215818.1 1458295 D 83332 CDS NP_215819.1 15608443 886944 1459766..1460251 1 NC_000962.3 Rv1303, (MTCY373.23), len: 161 aa. Conserved hypothetical transmembrane protein, highly similar to P53431|Y02N_MYCLE hypothetical Mycobacterium leprae protein (153 aa), FASTA score: opt: 636, E():0, (69.8% identity in 149 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1460251 886944 Rv1303 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215819.1 1459766 D 83332 CDS NP_215820.1 15608444 886941 1460244..1460996 1 NC_000962.3 Rv1304, (MTCY373.24), len: 250 aa. Probable atpB,ATP synthase a chain, highly similar to ATP6_MYCLE|P45829 Mycobacterium leprae (251 aa), FASTA scores: opt: 1382,E(): 0, (84.0% identity in 250 aa overlap). Contains PS00449 ATP synthase a subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1),epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase a chain family.; Probable ATP synthase a chain AtpB (protein 6) 1460996 atpB 886941 atpB Mycobacterium tuberculosis H37Rv Probable ATP synthase a chain AtpB (protein 6) NP_215820.1 1460244 D 83332 CDS NP_215821.1 15608445 886937 1461045..1461290 1 NC_000962.3 Rv1305, (MTCY373.25), len: 81 aa. Probable atpE, ATP synthase C chain, highly similar to P45828|ATPL_MYCLE Mycobacterium leprae (92.6% identity in 81 aa overlap). Contains PS00605 ATP synthase C subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1),epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase C chain family.; Probable ATP synthase C chain AtpE (lipid-binding protein) (dicyclohexylcarbodiimide-binding protein) 1461290 atpE 886937 atpE Mycobacterium tuberculosis H37Rv Probable ATP synthase C chain AtpE (lipid-binding protein) (dicyclohexylcarbodiimide-binding protein) NP_215821.1 1461045 D 83332 CDS NP_215822.1 15608446 886939 1461321..1461836 1 NC_000962.3 Rv1306, (MTCY373.26), len: 171 aa. Probable atpF,ATP synthase B chain, highly similar to ATPF_MYCLE P45827 (170 aa), FASTA scores, opt: 802, E(): 0, (79.5% identity in 171 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3),beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase B chain family.; Probable ATP synthase B chain AtpF 1461836 atpF 886939 atpF Mycobacterium tuberculosis H37Rv Probable ATP synthase B chain AtpF NP_215822.1 1461321 D 83332 CDS NP_215823.1 15608447 886934 1461843..1463183 1 NC_000962.3 Rv1307, (MTCY373.27), len: 446 aa. Probable atpH,ATP synthase delta chain. This protein is much longer than that of other bacterial delta chains, the C-terminal region is homologous to delta chains while the N-terminal region is similar to B/B' subunits e.g. ATPD_STRLI|P50008 ATP synthase delta chain from Streptomyces lividans (273 aa),FASTA scores: opt: 505, E(): 5.4e-23, (35.0% identity in 277 aa overlap); and ATPF_HAEIN|P43720 ATP synthase B chain from Haemophilus influenzae (156 aa), FASTA scores: opt: 216, E(): 1.2e-06, (26.1% identity in 153 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1),delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase delta chain family.; Probable ATP synthase delta chain AtpH 1463183 atpH 886934 atpH Mycobacterium tuberculosis H37Rv Probable ATP synthase delta chain AtpH NP_215823.1 1461843 D 83332 CDS NP_215824.1 15608448 886936 1463228..1464877 1 NC_000962.3 Rv1308, (MTCY373.28), len: 549 aa. Probable atpA,ATP synthase alpha chain, highly similar to ATPA_MYCLE|P45825 from Mycobacterium leprae (558 aa), FASTA scores: opt: 3233, E(): 0, (90.3% identity in 547 aa overlap). Contains PS00017 ATP/GTP-binding site motif A,PS00152 ATP synthase alpha and beta subunits signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1),delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase alpha/beta chains family.; Probable ATP synthase alpha chain AtpA 1464877 atpA 886936 atpA Mycobacterium tuberculosis H37Rv Probable ATP synthase alpha chain AtpA NP_215824.1 1463228 D 83332 CDS NP_215825.1 15608449 886929 1464884..1465801 1 NC_000962.3 Rv1309, (MTCY373.29), len: 305 aa. Probable atpG,ATP synthase gamma chain, highly similar to ATPG_MYCLE|P45824 ATP synthase gamma chain from Mycobacterium leprae (298 aa), FASTA scores: opt: 1579,E():0, (83.9% identity in 305 aa overlap). Contains PS00153 ATP synthase gamma subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1),epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase gamma chain family.; Probable ATP synthase gamma chain AtpG 1465801 atpG 886929 atpG Mycobacterium tuberculosis H37Rv Probable ATP synthase gamma chain AtpG NP_215825.1 1464884 D 83332 CDS NP_215826.1 15608450 886932 1465841..1467301 1 NC_000962.3 Rv1310, (MTCY373.30), len: 486 aa. Probable atpD,ATP synthase beta chain, highly similar to ATPB_MYCLE|P45823 Mycobacterium leprae (485 aa), FASTA score: opt: 2916, E(): 0, (92.6% identity in 484 aa overlap). Contains PS00017 ATP/GTP-binding site motif A,PS00152 ATP synthase alpha and beta subunits signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1),delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase alpha/beta chains family.; Probable ATP synthase beta chain AtpD 1467301 atpD 886932 atpD Mycobacterium tuberculosis H37Rv Probable ATP synthase beta chain AtpD NP_215826.1 1465841 D 83332 CDS NP_215827.1 15608451 886967 1467315..1467680 1 NC_000962.3 Rv1311, (MTCY373.31), len: 121 aa. Probable atpC,ATP synthase epsilon chain, highly similar to ATPE_MYCLE|P45822 Mycobacterium leprae (124 aa), FASTA scores: opt: 682, E(): 5.4e-40, (87.6% identity in 121 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3),gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase epsilon chain family.; Probable ATP synthase epsilon chain AtpC 1467680 atpC 886967 atpC Mycobacterium tuberculosis H37Rv Probable ATP synthase epsilon chain AtpC NP_215827.1 1467315 D 83332 CDS NP_215828.1 15608452 886930 1467688..1468131 1 NC_000962.3 Rv1312, (MTCY373.32), len: 147 aa. Conserved hypothetical secreted protein with potential N-terminal signal sequence. Highly similar to P53432|Y02W_MYCLE hypothetical Mycobacterium leprae protein (147 aa), FASTA score: opt: 884, E(): 0, (88.4% identity in 147 aa overlap). N-terminus hydrophobic.; Conserved hypothetical secreted protein 1468131 886930 Rv1312 Mycobacterium tuberculosis H37Rv Conserved hypothetical secreted protein NP_215828.1 1467688 D 83332 CDS NP_215829.1 15608453 886922 complement(1468171..1469505) 1 NC_000962.3 Rv1313c, (MTCY373.33c), len: 444 aa. Possible IS1557 transposase, similar to several transposases e.g. U57649|DBU57649 ORF1 from dibenzofuran-degrading bacterium DPO360 (163 aa), FASTA scores: opt: 767, E(): 0, (67.3% identity in 168 aa overlap); TNPA_BORPA|Q06126 transposase for insertion sequence element IS1001 from Bordetella parapertussis (406 aa), FASTA scores: opt: 254, E(): 3.3e-10, (24.9% identity in 402 aa overlap). Also similar to putative Mycobacterium tuberculosis transposases, Rv3798 and Rv0741.; Possible transposase 1469505 886922 Rv1313c Mycobacterium tuberculosis H37Rv Possible transposase NP_215829.1 1468171 R 83332 CDS NP_215830.1 15608454 886925 complement(1469671..1470252) 1 NC_000962.3 Rv1314c, (MTCY373.34c), len: 193 aa. Conserved protein, highly similar to P53523|Y02Y_MYCLE hypothetical Mycobacterium leprae protein (191 aa), FASTA score: opt:1019, E(): 0, (81.2% identity in 191 aa overlap). Some similarity with YDHW_CITFR|P45515 hypothetical 19.8 kDa protein in dhar-dhat intergenic region (176 aa), FASTA scores: opt: 297, E(): 1.6e-13, (37.6% identity in 178 aa overlap). Also similar to hypothetical protein AE002007|AE002007_3 Deinococcus radiodurans (185 aa), FASTA score: opt: 386, E(): 7.7e-19, (42.4% identity in 172 aa overlap).; hypothetical protein 1470252 886925 Rv1314c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215830.1 1469671 R 83332 CDS NP_215831.1 15608455 886921 1470321..1471577 1 NC_000962.3 Rv1315, (MTCY373.35-MTCY149.01), len: 418 aa. Probable murA, UDP-N-acetylglucosamine 1-carboxyvinyltransferase (see Belanger & Inamine 2000),highly similar to many e.g. MURA_MYCLE|P45821 (418 aa),FASTA scores: opt: 2495, E(): 0, (96.2% identity in 396 aa overlap). Belongs to the EPSP synthase family. MURA subfamily.; Probable UDP-N-acetylglucosamine 1-carboxyvinyltransferase MurA 1471577 murA 886921 murA Mycobacterium tuberculosis H37Rv Probable UDP-N-acetylglucosamine 1-carboxyvinyltransferase MurA NP_215831.1 1470321 D 83332 CDS NP_215832.1 15608456 886913 complement(1477134..1477631) 1 NC_000962.3 Rv1316c, (MTCY130.01c), len: 165 aa. Ogt,methylated-dna--protein-cysteine methytransferase (see citation below), similar to many e.g. OGT_HAEIN|P44687 Haemophilus influenzae (190 aa), FASTA scores: opt: 405,E(): 6.5e-20, (41.9% identity in 155 aa overlap). Contains PS00374 Methylated-DNA--protein-cysteine methyltransferase active site.; Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase) 1477631 ogt 886913 ogt Mycobacterium tuberculosis H37Rv Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase) NP_215832.1 1477134 R 83332 CDS NP_215833.1 15608457 886916 complement(1477628..1479118) 1 NC_000962.3 Rv1317c, (MTCY130.02c), len: 496 aa. Probable alkA (alternate gene name: ada), bifunctional regulatory protein (see citation below) and DNA repair enzyme, similar to 3MG2_ECOLI|P04395 dna-3-methyladenine glycosidase II from Escherichia coli (282 aa), FASTA scores, opt: 437, E(): 8.6e-22, (32.8% identity in 293 aa overlap), also similar to other ada proteins e.g. ADA_SALTY|P26189 Salmonella typhimurium (352 aa), FASTA scores: E(): 5.3e-08, (35.9% identity in 156 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature.; Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase) 1479118 alkA 886916 alkA Mycobacterium tuberculosis H37Rv Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase) NP_215833.1 1477628 R 83332 CDS NP_215834.1 15608458 886910 complement(1479199..1480824) 1 NC_000962.3 Rv1318c, (MTCY130.03c), len: 541 aa. Possible adenylate cyclase. Some similarity at the c-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores, opt: 270, E(): 2.5e-11, (28.8% identity in 184 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap), also similar to Rv1320c|MTCY130.05c (567 aa), FASTA scores, opt: 2423, E(): 0, (68.7% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family.; Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) 1480824 886910 Rv1318c Mycobacterium tuberculosis H37Rv Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) NP_215834.1 1479199 R 83332 CDS NP_215835.1 15608459 886911 complement(1480894..1482501) 1 NC_000962.3 Rv1319c, (MTCY130.04c), len: 535 aa. Possible adenylate cyclase. Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 254, E(): 2.4e-10, (33.3% identity in 144 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap); Rv1320c|MTCY130.05c (567 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family.; Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) 1482501 886911 Rv1319c Mycobacterium tuberculosis H37Rv Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) NP_215835.1 1480894 R 83332 CDS NP_215836.1 15608460 886906 complement(1482514..1484217) 1 NC_000962.3 Rv1320c, (MTCY130.05c), len: 567 aa. Possible adenylate cyclase (see Rindi et al., 1999). Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 277,E(): 2e-12, (34.0% identity in 156 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2423,E(): 0, (68.7% identity in 534 aa overlap); Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family.; Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) 1484217 886906 Rv1320c Mycobacterium tuberculosis H37Rv Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) NP_215836.1 1482514 R 83332 CDS NP_215837.1 15608461 886908 1484279..1484959 1 NC_000962.3 Rv1321, (MTCY130.06), len: 226 aa. Conserved hypothetical protein. Equivalent to P53524|YD21_MYCLE hypothetical protein from Mycobacterium leprae (201 aa),FASTA scores: opt: 1144, E(): 0, (87.6% identity in 193 aa overlap). Some similarity to hypothetical proteins from other organisms e.g. Y225_METJA|Q57678 Methanococcus jannaschii (263 aa), FASTA scores: E(): 6.5e-05, (25.0% identity in 212 aa overlap).; hypothetical protein 1484959 886908 Rv1321 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215837.1 1484279 D 83332 CDS NP_215838.1 15608462 886927 1484982..1485278 1 NC_000962.3 Rv1322, (MTCY130.07), len: 98 aa. Conserved hypothetical protein.; hypothetical protein 1485278 886927 Rv1322 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215838.1 1484982 D 83332 CDS YP_177643.1 57116847 3205097 complement(1485313..1485771) 1 NC_000962.3 Rv1322A, len: 152 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. AL583921_2|ML1157 from M. leprae strain tn (155 aa), FASTA scores: opt: 771, E(): 5.1e-43, (75.3% identity in 154 aa overlap); and AL137242_2 from Streptomyces coelicolor (146 aa), FASTA scores: opt: 404,E(): 2e-19, (43.165% identity in 139 aa overlap).; hypothetical protein 1485771 3205097 Rv1322A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177643.1 1485313 R 83332 CDS NP_215839.1 15608463 886904 1485862..1487031 1 NC_000962.3 Rv1323, (MTCY130.08), len: 389 aa. Probable fadA4,acetyl-CoA acetyltransferase, equivalent to THIL_MYCLE|P46707 possible acetyl-CoA C-acetyltransferase from Mycobacterium leprae (393 aa), FASTA scores: opt: 2218, E(): 0, (87.0% identity in 392 aa overlap). Also highly similar to others e.g. CAB70629.1|AL137242 probable acetoacetyl-CoA thiolase from Streptomyces coelicolor (401 aa); T51772 acetyl-CoA C-acetyltransferase [validated] from Alcaligenes latus (392 aa); etc. Some homologies indicate ATA start codon. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2, and PS00099 Thiolases active site. Belongs to the thiolase family.; Probable acetyl-CoA acetyltransferase FadA4 (acetoacetyl-CoA thiolase) 1487031 fadA4 886904 fadA4 Mycobacterium tuberculosis H37Rv Probable acetyl-CoA acetyltransferase FadA4 (acetoacetyl-CoA thiolase) NP_215839.1 1485862 D 83332 CDS NP_215840.1 15608464 886897 1487161..1488075 1 NC_000962.3 Rv1324, (MTCY130.09), len: 304 aa. Possible thioredoxin, similar to several e.g. U00014|Q49716 TRXA from Mycobacterium leprae (255 aa), FASTA scores: opt: 1014, E(): 0, (69.7% identity in 228 aa overlap); THIO_RHOSH|P08058 TrxA from Rhodobacter sphaeroides (105 aa), FASTA scores: opt 196, E(): 1.9e-06, (33.0% identity in 103 aa overlap). Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible thioredoxin 1488075 886897 Rv1324 Mycobacterium tuberculosis H37Rv Possible thioredoxin NP_215840.1 1487161 D 83332 CDS YP_177799.1 57116848 886899 complement(1488154..1489965) 1 NC_000962.3 Rv1325c, (MTCY130.10c), len: 603 aa. PE_PGRS24,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of ala-, gly-rich proteins (see Brennan & Delogu 2002), similar to many e.g. YQ04_MYCTU|P71933 hypothetical 63.1 kDa glycine-rich protein (778 aa), FASTA scores: E(): 0, (52.3% identity in 724 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; PE-PGRS family protein PE_PGRS24 1489965 PE_PGRS24 886899 PE_PGRS24 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS24 YP_177799.1 1488154 R 83332 CDS NP_215842.1 15608466 886893 complement(1490117..1492312) 1 NC_000962.3 Rv1326c, (MTCY130.11c), len: 731 aa. glgB,1,4-alpha-glucan branching enzyme, similar to others e.g. GLGB_ECOLI|P07762 Escherichia coli (728 aa), FASTA scores: opt: 2330, E(): 0, (48.7% identity in 719 aa overlap). Similar to other Mycobacterium tuberculosis putative alpha-glucan branching enzymes Rv1562c, Rv1563c. Belongs to family 13 of glycosyl hydrolases, also known as the alpha-amylase family.; 1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme) 1492312 glgB 886893 glgB Mycobacterium tuberculosis H37Rv 1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme) NP_215842.1 1490117 R 83332 CDS NP_215843.2 57116849 886895 complement(1492320..1494425) 1 NC_000962.3 Rv1327c, (MTCY130.12c), len: 701 aa. Probable glgE,glucanase, similar to AF172946|AF172946_2 putative glucanase GlgE from Mycobacterium smegmatis (697 aa), FASTA scores: opt: 3816, E(): 0, (78.5% identity in 692 aa overlap). Similar to putative alpha-amylases e.g. Q9L1K2 Streptomyces coelicolor (675 aa), FASTA scores: opt: 2243,E(): 7.4e-132, (54.2% identity in 684 aa overlap). Start changed since original submission (-36) based on similarity to GlgE of Mycobacterium smegmatis; previous start at position 1494531.; Probable glucanase GlgE 1494425 glgE 886895 glgE Mycobacterium tuberculosis H37Rv Probable glucanase GlgE NP_215843.2 1492320 R 83332 CDS NP_215844.1 15608468 886886 1494564..1497155 1 NC_000962.3 Rv1328, (MTCY130.13), len: 863 aa. Probable glgP,glycogen phosphorylase, similar to many e.g. PHSG_HAEIN|P45180 glycogen phosphorylase from Haemophilus influenzae (821 aa), FASTA scores: E(): 6.9e-08, (25.6% identity in 675 aa overlap). Belongs to the glycogen phosphorylase family.; Probable glycogen phosphorylase GlgP 1497155 glgP 886886 glgP Mycobacterium tuberculosis H37Rv Probable glycogen phosphorylase GlgP NP_215844.1 1494564 D 83332 CDS NP_215845.1 15608469 886889 complement(1497195..1499189) 1 NC_000962.3 Rv1329c, (MTCY130.14c), len: 664 aa. Probable dinG,ATP-dependent helicase (see citation below), similar to several e.g. DING_HAEIN|P44680 probable ATP-dependent helicase ding from Haemophilus influenzae (640 aa), FASTA scores: opt: 685, E(): 2.3e-38, (32.8% identity in 644 aa overlap). Contains PS00017 ATP/GTP-binding site motif A.; Probable ATP-dependent helicase DinG 1499189 dinG 886889 dinG Mycobacterium tuberculosis H37Rv Probable ATP-dependent helicase DinG NP_215845.1 1497195 R 83332 CDS NP_215846.2 57116850 886884 complement(1499213..1500559) 1 NC_000962.3 Rv1330c, (MTCY130.15c), len: 448 aa. PncB1,nicotinic acid phosphoribosyltransferase (See Boshoff et al., 2008). Similar to e.g. O32090 YUEK protein from Bacillus subtilis (490 aa), FASTA scores: E(): 8.6e-22,(37.9% identity in 369 aa overlap). Also similar to Mycobacterium tuberculosis Rv0573c|MTV039.11c (38.0% identity in 437 aa overlap). Start changed since original submission based on similarity; previous start at position 1500740 (-61 aa).; Nicotinic acid phosphoribosyltransferase PncB1 1500559 pncB1 886884 pncB1 Mycobacterium tuberculosis H37Rv Nicotinic acid phosphoribosyltransferase PncB1 NP_215846.2 1499213 R 83332 CDS NP_215847.1 15608471 886891 1500661..1500966 1 NC_000962.3 Rv1331, (MTCY130.16), len: 101 aa. Conserved hypothetical protein, highly similar to U00014|ML014 B1549_C2_207 from Mycobacterium leprae (94 aa), FASTA scores: opt: 573, E(): 2.9e-40, (90.3% identity in 93 aa overlap). Similar to AL096852|SCE19A_16 hypothetical protein from Streptomyces coelicolor (105 aa), FASTA scores: opt: 377, E(): 2.9e-22, (60.0% identity in 105 aa overlap).; hypothetical protein 1500966 886891 Rv1331 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215847.1 1500661 D 83332 CDS NP_215848.1 15608472 886901 1500926..1501582 1 NC_000962.3 Rv1332, (MTCY130.17), len: 218 aa. Possible regulatory protein, high similarity to ML014|U00014 M. leprae B1549_C3_236 (222 aa), FASTA scores: opt: 1158, E(): 0, (75.6% identity in 221 aa overlap). Helix turn helix motif fram aa 8-29 (+3.03 SD).; Probable transcriptional regulatory protein 1501582 886901 Rv1332 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215848.1 1500926 D 83332 CDS NP_215849.1 15608473 886882 1501599..1502633 1 NC_000962.3 Rv1333, (MTCY130.18), len: 344 aa. Possible hydrolase, similar to Q57326|D26094 endo-type 6-aminohexanoate oligomer hydrolase (355 aa), fasta scores: E(): 1.4e-10, (31.9% identity in 339 aa overlap). Equivalent to P53425|YD33_MYCLE hypothetical 36.1 KD protein B154 Mycobacterium leprae (362 aa), FASTA scores: opt: 1735, E(): 0, (76.7% identity in 352 aa overlap).; Probable hydrolase 1502633 886882 Rv1333 Mycobacterium tuberculosis H37Rv Probable hydrolase NP_215849.1 1501599 D 83332 CDS NP_215850.1 15608474 886875 1502641..1503081 1 NC_000962.3 Rv1334, (MTCY130.19), len: 146 aa. Possible mec,hydrolase (See Burns et al., 2005), similar to AL096852|SCE19A_13 hypothetical protein from Streptomyces coelicolor (140 aa), Fasta scores: opt: 579, E(): 0, (65.0% identity in 140 aa overlap); and Q54330|M29166 MEC+ from Streptomyces kasugaensis (115 aa), FASTA scores; E(): 7.6e-33, (56.9% identity in 109 aa overlap).; Possible hydrolase 1503081 mec 886875 mec Mycobacterium tuberculosis H37Rv Possible hydrolase NP_215850.1 1502641 D 83332 CDS NP_215851.1 15608475 886879 1503103..1503384 1 NC_000962.3 Rv1335, (MT1376.1, MTCY130.20), len: 93 aa. CysO,sulfur carrier protein (See Burns et al., 2005). Note that previously known as cfp10A. Similar to hypothetical proteins from other organisms e.g. P74060|D90911 Synechocystis (109 aa), FASTA scores: E(): 2.3e-20, (49.5% identity in 93 aa overlap).; Sulfur carrier protein CysO 1503384 cysO 886879 cysO Mycobacterium tuberculosis H37Rv Sulfur carrier protein CysO NP_215851.1 1503103 D 83332 CDS NP_215852.1 15608476 886867 1503394..1504365 1 NC_000962.3 Rv1336, (MTCY130.21), len: 323 aa. cysM, cysteine synthase B, similar to many e.g. CYSM_ECOLI|P16703 Escherichia coli (303 aa), FASTA scores: opt: 720, E(): 4.6e-40, (41.1% identity in 302 aa overlap). Also similar to other Mycobacterium tuberculosis cysteine synthase subunits e.g. Rv1077, Rv2334, Rv0848, etc. Contains PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. Belongs to the cysteine synthase/cystathionine beta-synthase family.; Cysteine synthase B CysM (CSASE B) (O-phosphoserine sulfhydrylase B) (O-phosphoserine (thiol)-lyase B) 1504365 cysM 886867 cysM Mycobacterium tuberculosis H37Rv Cysteine synthase B CysM (CSASE B) (O-phosphoserine sulfhydrylase B) (O-phosphoserine (thiol)-lyase B) NP_215852.1 1503394 D 83332 CDS NP_215853.1 15608477 886870 1504356..1505078 1 NC_000962.3 Rv1337, (MTCY130.22), len: 240 aa. Probable integral membrane protein. Highly similar to P53426 hypothetical protein B1549_C3_240 from M.leprae (251); and P74553|D90916 hypothetical protein from Synechocystis sp. (198 aa), FASTA scores: E(): 2.3e-25, (43.6% identity in 181 aa overlap).; Probable integral membrane protein 1505078 886870 Rv1337 Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_215853.1 1504356 D 83332 CDS NP_215854.1 15608478 886866 1505075..1505890 1 NC_000962.3 Rv1338, (MTCY130.23), len: 271 aa. Probable murI,glutamate racemase, highly similar to many e.g. MURI_MYCLE|P46705 (272 aa), FASTA scores: opt: 1559, E(): 0, (88.9% identity in 271 aa overlap). Contains PS00924 Aspartate and glutamate racemases signature 2.; Probable glutamate racemase MurI 1505890 murI 886866 murI Mycobacterium tuberculosis H37Rv Probable glutamate racemase MurI NP_215854.1 1505075 D 83332 CDS NP_215855.1 15608479 886972 1505917..1506738 1 NC_000962.3 Rv1339, (MTCY130.24), len: 273 aa. Conserved protein, highly similar to Y211_MYCLE|P50474 hypothetical protein b1549_c2_211 from Mycobacterium leprae (284 aa),FASTA scores: opt: 1672, E(): 0, (86.2% identity in 276 aa overlap). Also similar to AL096852|SCE19A.08 hypothetical protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 630, E(): 0, (42.2% identity in 256 aa overlap). Similar to M. tuberculosis hypothetical proteins Rv3796, Rv2407. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 1506738 886972 Rv1339 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215855.1 1505917 D 83332 CDS NP_215856.1 15608480 886864 1506755..1507534 1 NC_000962.3 Rv1340, (MTCY130.25), len: 259 aa. Probable rphA,Ribonuclease ph, highly similar to others e.g. RNPH_MYCLE|P37939 Mycobacterium leprae (259 aa), FASTA scores: opt: 1524, E(): 0, (88.8% identity in 259 aa overlap). Belongs to the RNASE PH family.; Probable ribonuclease RphA (RNase PH) (tRNA nucleotidyltransferase) 1507534 rphA 886864 rphA Mycobacterium tuberculosis H37Rv Probable ribonuclease RphA (RNase PH) (tRNA nucleotidyltransferase) NP_215856.1 1506755 D 83332 CDS NP_215857.1 15608481 886861 1507573..1508187 1 NC_000962.3 Rv1341, (MTCY130.26), len: 204 aa. Conserved protein, some similarity to P52061|YGGV_ECOLI hypothetical protein yggV (197 aa), FASTA scores: opt: 521, E(): 7.9e-27, (46.0% identity in 200 aa overlap). Equivalent to ML014|U00014 hypothetical protein B1549_C2_213 from Mycobacterium leprae (285 aa), FASTA scores: opt: 1073,E(): 0, (83.0% identity in 206 aa overlap).; hypothetical protein 1508187 886861 Rv1341 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215857.1 1507573 D 83332 CDS YP_177800.1 57116851 886862 complement(1508184..1508546) 1 NC_000962.3 Rv1342c, (MTCY02B10.06c), len: 120 aa. Conserved membrane protein. Highly similar to G466926|P54133 hypothetical protein B1549_F2_59 from Mycobacterium leprae (119 aa), FASTA scores, opt: 544, E(): 1.9e-29, (68.3 % identity in 120 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 1508546 886862 Rv1342c Mycobacterium tuberculosis H37Rv hypothetical protein YP_177800.1 1508184 R 83332 CDS NP_215859.1 15608483 886852 complement(1508543..1508923) 1 NC_000962.3 Rv1343c, (MTCY02B10.07c), len: 126 aa. Probable lprD, conserved lipoprotein, highly similar to G466928 Mycobacterium leprae protein B1549_F3_106 (126 aa), FASTA scores, opt: 704, E(): 7.5e-36, (78.4 % identity in 125 aa overlap). Has N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein attachment site. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved lipoprotein LprD 1508923 lprD 886852 lprD Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LprD NP_215859.1 1508543 R 83332 CDS NP_215860.1 15608484 886848 1508968..1509288 1 NC_000962.3 Rv1344, (MTCY02B10.08), len: 106 aa. mbtL, acyl carrier protein, similar to others e.g. ACP_RHIME|P19372 Rhizobium meliloti (77 aa), FASTA scores: opt: 117, E(): 0.03, (29.9% identity in 67 aa overlap) and ACP_SYNY3|P20804 acyl carrier protein (acp) from Synechocystis sp (77 aa), FASTA scores: E(): 7.1e-05,(34.8% identity in 66 aa overlap). Also similar to Rv2244 and Rv0033 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Acyl carrier protein (ACP) MbtL 1509288 mbtL 886848 mbtL Mycobacterium tuberculosis H37Rv Acyl carrier protein (ACP) MbtL NP_215860.1 1508968 D 83332 CDS NP_215861.1 15608485 886855 1509281..1510846 1 NC_000962.3 Rv1345, (MTCY02B10.09), len: 521 aa. Probable mbtM,fatty acyl-AMP ligase. Similar to N-terminus of T34918 polyketide synthase from Streptomyces coelicolor (2297 aa); and PKSJ_BACSU|P40806 putative polyketide biosynthesis protein from Bacillus subtilis (557 aa), FASTA scores: opt: 537, E(): 8.2e-27, (27.1% identity in 468 aa overlap). Also similar to other proteins from Mycobacterium tuberculosis eg Rv1013|MTCI237.30|MTCY10G2.36c|pks16 putative polyketide synthase (544 aa); etc. Note that previously known as fadD33.; Probable fatty acyl-AMP ligase MbtM 1510846 mbtM 886855 mbtM Mycobacterium tuberculosis H37Rv Probable fatty acyl-AMP ligase MbtM NP_215861.1 1509281 D 83332 CDS NP_215862.1 15608486 886844 1510846..1512006 1 NC_000962.3 Rv1346, (MTCY02B10.10), len: 386 aa. mbtN, acyl-CoA dehydrogenase, similar to many e.g. NP_251579.1|NC_002516 probable acyl-CoA dehydrogenase from Pseudomonas aeruginosa (386 aa); NP_036951.1|NM_012819|ACDL_RAT|P15650 acyl Coenzyme A dehydrogenase (long chain) from Rattus norvegicus (430 aa), FASTA scores: opt: 414, E(): 1.2e-18,(26.1% identity in 376 aa overlap); etc. Note that previously known as fadE14.; Acyl-CoA dehydrogenase MbtN 1512006 mbtN 886844 mbtN Mycobacterium tuberculosis H37Rv Acyl-CoA dehydrogenase MbtN NP_215862.1 1510846 D 83332 CDS NP_215863.1 15608487 886846 complement(1511973..1512605) 1 NC_000962.3 Rv1347c, (MTCY02B10.11c), len: 210 aa. MbtK, lysine N-acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to the C-terminus of malonyl-coenzyme A carboxylases e.g. G545170 malonyl-coenzyme A carboxylase (417 aa), FASTA scores: opt: 392, E(): 4.9 e-20, (35.6% identity in 174 aa overlap).; Lysine N-acetyltransferase MbtK 1512605 mbtK 886846 mbtK Mycobacterium tuberculosis H37Rv Lysine N-acetyltransferase MbtK NP_215863.1 1511973 R 83332 CDS NP_215864.1 15608488 886853 1513047..1515626 1 NC_000962.3 Rv1348, (MTCY02B10.12), len: 859 aa. IrtA,iron-regulated transporter. Probable transmembrane protein,similar to HMT1_SCHPO|Q02592 heavy metal tolerance protein precursor from Schizosaccharomyces pombe (830 aa), FASTA scores: opt: 806, E(): 5.1e-39, (32.9% identity in 504 aa overlap); etc. Also similar to MTCY02B10.13 from Mycobacterium tuberculosis, FASTA score: (31.9% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Cofactor: FAD; Iron-regulated transporter IrtA 1515626 irtA 886853 irtA Mycobacterium tuberculosis H37Rv Iron-regulated transporter IrtA NP_215864.1 1513047 D 83332 CDS NP_215865.1 15608489 886834 1515623..1517362 1 NC_000962.3 Rv1349, (MTCY02B10.13), len: 579 aa. IrtB,iron-regulated transporter. Probable transmembrane protein,most similar to YWJA_BACSU|P45861 hypothetical ABC transporter from Bacillus subtilis (575 aa), FASTA scores: opt: 721, E(): 1.8e-35, (28.9% identity in 567 aa overlap); etc. Also similar to MTCY02B10.12 from Mycobacterium tuberculosis, FASTA score: (31.9% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Predicted possible vaccine candidate (See Zvi et al., 2008).; Iron-regulated transporter IrtB 1517362 irtB 886834 irtB Mycobacterium tuberculosis H37Rv Iron-regulated transporter IrtB NP_215865.1 1515623 D 83332 CDS NP_215866.1 15608490 886837 1517491..1518234 1 NC_000962.3 Rv1350, (MTCY02B10.14), len: 247 aa. Probable fabG2,3-oxoacyl-[acyl-carrier protein] reductase, highly similar to many e.g. NP_350157.1|NC_003030 3-ketoacyl-acyl carrier protein reductase from Clostridium acetobutylicum (249 aa); NP_229523.1|NC_000853 3-oxoacyl-(acyl carrier protein) reductase from Thermotoga maritima (246 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG2 (3-ketoacyl-acyl carrier protein reductase) 1518234 fabG2 886837 fabG2 Mycobacterium tuberculosis H37Rv Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG2 (3-ketoacyl-acyl carrier protein reductase) NP_215866.1 1517491 D 83332 CDS NP_215867.1 15608491 886831 1518231..1518560 1 NC_000962.3 Rv1351, (MTCY02B10.15), len: 109 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 1518560 886831 Rv1351 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215867.1 1518231 D 83332 CDS NP_215868.1 15608492 886833 1518763..1519134 1 NC_000962.3 Rv1352, (MTCY02B10.16), len: 123 aa. Conserved protein, some similarity to Rv1906c|MTCY180.12 hypothetical protein from Mycobacterium tuberculosis (156 aa), FASTA scores: E(): 4e-05, (36.2% identity in 116 aa overlap).; hypothetical protein 1519134 886833 Rv1352 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215868.1 1518763 D 83332 CDS NP_215869.1 15608493 886827 complement(1519200..1519985) 1 NC_000962.3 Rv1353c, (MTCY02B10.17c), len: 261 aa. Probable transcriptional regulatory protein, similar to TER1_ECOLI|P03038 tetracycline repressor protein class a from Escherichia coli (216 aa), FASTA scores, opt: 231,E(): 1.6e-08, (31.3% identity in 211 aa overlap). Helix turn helix motif present at aa 3859 (+3.59 SD). Belongs to the TetR/AcrR family of transcriptional regulators.; Probable transcriptional regulatory protein 1519985 886827 Rv1353c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215869.1 1519200 R 83332 CDS NP_215870.1 15608494 886829 complement(1520005..1521876) 1 NC_000962.3 Rv1354c, (MTCY02B10.18c), len: 623 aa. Conserved hypothetical protein, similar to many hypothetical proteins e.g. the C-terminus of G1001455 Synechocystis sp. (1244 aa), FASTA scores: opt: 933, E(): 0, (36.8% identity in 462 aa overlap); also similar to Rv1357c|MTCY02B10.21c (34.0% identity in 253 aa overlap).; hypothetical protein 1521876 886829 Rv1354c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215870.1 1520005 R 83332 CDS NP_215871.1 15608495 886839 complement(1521885..1524032) 1 NC_000962.3 Rv1355c, (MTCY02B10.19c), len: 715 aa. Possible moeY, Molybdopterin biosynthesis protein, very weak similarity to MOEB_ECOLI|P12282 molybdopterin biosynthesis moeb protein (249 aa), FASTA scores, opt: 180, E(): 8.5e-05, (29.3% identity in 174 aa overlap).; Possible molybdopterin biosynthesis protein MoeY 1524032 moeY 886839 moeY Mycobacterium tuberculosis H37Rv Possible molybdopterin biosynthesis protein MoeY NP_215871.1 1521885 R 83332 CDS NP_215872.1 15608496 886850 complement(1524029..1524820) 1 NC_000962.3 Rv1356c, (MTCY02B10.20c), len: 263 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 1524820 886850 Rv1356c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215872.1 1524029 R 83332 CDS NP_215873.1 15608497 886815 complement(1525293..1526216) 1 NC_000962.3 Rv1357c, (MTCY02B10.21c), len: 307 aa. Conserved hypothetical protein, similar to members of the YEGE/YHJK/YJCC family e.g. Y4LL_RHISN|P55552 hypothetical protein Y4ll from Rhizobium sp. (827 aa), FASTA scores: E(): 0, (37.7% identity in 257 aa overlap), also similar to Rv1354c|MTCY02B10.18c (34.0% identity in 253 aa overlap). Belongs to the YEGE/YHDA/YHJK/YJCC family.; hypothetical protein 1526216 886815 Rv1357c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215873.1 1525293 R 83332 CDS NP_215874.1 15608498 886817 1526612..1530091 1 NC_000962.3 Rv1358, (MTCY02B10.22), len: 1159 aa. Probable transcriptional regulatory protein, some similarity to AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 210, E(): 5.5e-06,(27.5% identity in 739 aa overlap). Similar also to Rv0890C|MTCY31.18c (65.5% identity in 884 aa overlap) and to Rv1359|MTCY02B10.23 (43.7% identity in 197 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00622 Bacterial regulatory proteins, luxR family signature. Helix turn helix motif present at aa 1116-1137, (Score 1291,+3.59 SD).; Probable transcriptional regulatory protein 1530091 886817 Rv1358 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215874.1 1526612 D 83332 CDS NP_215875.1 15608499 886841 1530173..1530925 1 NC_000962.3 Rv1359, (MTCY02B10.23), len: 250 aa. Probable transcriptional regulatory protein, similar to Rv0891c|MTCY31.19c, (48.5% identity in 204 aa overlap) and to Rv1358|MTCY02B10.22 (43.7% identity in 197 aa overlap).; Probable transcriptional regulatory protein 1530925 886841 Rv1359 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215875.1 1530173 D 83332 CDS NP_215876.1 15608500 886813 1531348..1532370 1 NC_000962.3 Rv1360, (MTCY02B10.24), len: 340 aa. Probable oxidoreductase. Similar to Q49598|G1002714 coenzyme F420-dependent n5, n10-methylenetetrahydromethanopterin reductase from Methanopyrus kandleri (349 aa), FASTA scores: opt: 264, E(): 4.4e-11, (26.3% identity in 323 aa overlap).; Probable oxidoreductase 1532370 886813 Rv1360 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_215876.1 1531348 D 83332 CDS YP_177801.1 57116852 886819 complement(1532443..1533633) 1 NC_000962.3 Rv1361c, (MTCY02B10.25c), len: 396 aa. PPE19 (alternate gene name: mtb39b). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to many e.g. Rv1196|MTCI364.08|PPE18, FASTA scores: E(): 0, (84.9% identity in 397 aa overlap); MTCY274.23c (42.3% identity in 416 aa overlap); etc. Contains PS00501 Signal peptidases I serine active site. Note that expression of Rv1361c was demonstrated in lysates by immunodetection (see Dillon et al., 1999). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; PPE family protein PPE19 1533633 PPE19 886819 PPE19 Mycobacterium tuberculosis H37Rv PPE family protein PPE19 YP_177801.1 1532443 R 83332 CDS NP_215878.1 15608502 886809 complement(1533948..1534610) 1 NC_000962.3 Rv1362c, (MTCY02B10.26c), len: 220 aa. Possible membrane protein, similar to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1362c|MTCY02B10.27c (25.9% identity in 216 aa overlap), Rv0177, Rv1973, Rv1972, etc.; Possible membrane protein 1534610 886809 Rv1362c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215878.1 1533948 R 83332 CDS NP_215879.1 15608503 886811 complement(1534607..1535392) 1 NC_000962.3 Rv1363c, (MTCY02B10.27c), len: 261 aa. Possible membrane protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv1362c|MTCY02B10.26c (25.9% identity in 216 aa overlap); Rv1972|MTV051.10 and Rv0177 etc.; Possible membrane protein 1535392 886811 Rv1363c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215879.1 1534607 R 83332 CDS YP_177802.1 57116853 886802 complement(1535683..1537644) 1 NC_000962.3 Rv1364c, (MTCY02B10.28c), len: 653 aa. Possible sigma factor regulatory protein, some similarity to RSBU_BACSU|P40399 sigma factor sibg regulation protein from Bacillus subtilis (335 aa), FASTA scores: opt: 224, E(): 2e-07, (25.8% identity in 244 aa overlap). Also known as mursiF.; Possible sigma factor regulatory protein 1537644 886802 Rv1364c Mycobacterium tuberculosis H37Rv Possible sigma factor regulatory protein YP_177802.1 1535683 R 83332 CDS NP_215881.1 15608505 886794 complement(1537783..1538169) 1 NC_000962.3 Rv1365c, (MTCY02B10.29c), len: 128 aa. RsfA,anti-anti-sigma factor (see citation below), similar to other Mycobacterium tuberculosis proteins e.g. Rv2638|MTCY441.08 (148 aa), FASTA scores: E(): 0, (53.6% identity in 125 aa overlap); Rv1904, Rv3687c. Weak similarity to putative anti-anti-sigma factors e.g. AF134889|AF134889_1 Streptomyces coelicolor (113 aa), FASTA scores: opt: 137, E(): 0.004, (26.0% identity in 100 aa overlap).; Anti-anti-sigma factor RsfA (anti-sigma factor antagonist) (regulator of sigma F A) 1538169 rsfA 886794 rsfA Mycobacterium tuberculosis H37Rv Anti-anti-sigma factor RsfA (anti-sigma factor antagonist) (regulator of sigma F A) NP_215881.1 1537783 R 83332 CDS NP_215882.1 15608506 886805 1538390..1539211 1 NC_000962.3 Rv1366, (MTCY02B10.30), len: 273 aa. Hypothetical unknown protein.; Hypothetical protein 1539211 886805 Rv1366 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215882.1 1538390 D 83332 CDS YP_004837053.2 448824755 14515857 1539180..1539440 1 NC_000962.3 Rv1366A, len: 86 aa. Conserved protein.; hypothetical protein 1539440 14515857 Rv1366A Mycobacterium tuberculosis H37Rv hypothetical protein YP_004837053.2 1539180 D 83332 CDS NP_215883.1 15608507 886793 complement(1539512..1540645) 1 NC_000962.3 Rv1367c, (MTCY02B12.01c,MTCY02B10.31c), len: 377 aa. Conserved protein. Some similarity to penicillin binding proteins e.g. PBPE_BACSU|P32959 penicillin-binding protein 4* (pbp 4*) from Bacillus subtilis (451 aa), FASTA scores: E(): 6.9e-06, (23.6% identity in 373 aa overlap). Similar to AL031107|SC5A7.06 hypothetical protein from Streptomyces coelicolor (409 aa), FASTA scores: opt: 675, E(): 0, (40.4% identity in 339 aa overlap).; hypothetical protein 1540645 886793 Rv1367c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215883.1 1539512 R 83332 CDS NP_215884.1 15608508 886798 1541020..1541805 1 NC_000962.3 Rv1368, (MTCY02B12.02), len: 261 aa. Probable lprF,conserved lipoprotein; similar to Mycobacterium tuberculosis hypothetical lipoproteins e.g. Rv1270c|Y08C_MYCTU|Q11049 hypothetical 26.4 kDa protein cy50.12. (257 aa), FASTA scores: opt: 286, E(): 5.3e-11,(26.3% identity in 270 aa overlap), also Rv1411c|MTCY21B4.28c, (32.8% identity in 253 aa overlap) and Rv2945c. Contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Belongs to the LPPX/lprafg family of lipoproteins.; Probable conserved lipoprotein LprF 1541805 lprF 886798 lprF Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LprF NP_215884.1 1541020 D 83332 CDS NP_215885.2 448824756 886789 complement(1541994..>1542980) 1 NC_000962.3 Rv1369c, (MTCY02B12.03c), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1368c and Rv1369c, the sequence UUUUAAAG (directly upstream of Rv1369c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa).; Probable transposase >1542980 886789 Rv1369c Mycobacterium tuberculosis H37Rv Probable transposase NP_215885.2 1541994 R 83332 CDS NP_215886.1 15608510 886791 complement(1542929..1543255) 1 NC_000962.3 Rv1370c, (MTCY02B12.04c), len: 108 aa. Putative transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43,(100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1368c and Rv1369c, the sequence UUUUAAAG (directly upstream of Rv1369c) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Putative transposase for insertion sequence element IS6110 (fragment) 1543255 886791 Rv1370c Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 (fragment) NP_215886.1 1542929 R 83332 CDS NP_215887.1 15608511 886800 1543359..1544828 1 NC_000962.3 Rv1371, (MTCY02B12.05), len: 489 aa. Probable membrane protein. Weak similarity to delta 5 fatty acid desaturases e.g. AB022097|AB022097_1 Dictyostelium discoideum (467 aa), FASTA score: opt: 173, E(): 0.00052,(22.4% identity in 438 aa overlap); and Homo sapiens.; Probable conserved membrane protein 1544828 886800 Rv1371 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_215887.1 1543359 D 83332 CDS YP_177803.1 57116854 886797 1544825..1546006 1 NC_000962.3 Rv1372, (MTCY02B12.06), len: 393 aa. Conserved hypothetical protein, similar to several chalcone synthases e.g. CHS2_GERHY|P48391 chalcone synthase 2 from gerbra hybrid (402 aa), FASTA scores: opt: 511, E(): 7e-26, (28.4% identity in 380 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical chalcone synthases, Rv1665,Rv1660.; hypothetical protein 1546006 886797 Rv1372 Mycobacterium tuberculosis H37Rv hypothetical protein YP_177803.1 1544825 D 83332 CDS NP_215889.1 15608513 886781 1546012..1546992 1 NC_000962.3 Rv1373, (MTCY02B12.07), len: 326 aa. Glycolipid sulfotransferase (see citation below); slight similarity to sulfotransferases e.g. SUOE_CAVPO|P49887 estrogen sulfotransferase from Cavia porcellus (Guinea pig) (296 aa), FASTA scores, opt: 165, E():0.00054, (24.5% identity in 294 aa overlap).; Glycolipid sulfotransferase 1546992 886781 Rv1373 Mycobacterium tuberculosis H37Rv Glycolipid sulfotransferase NP_215889.1 1546012 D 83332 CDS NP_215890.2 57116855 886783 complement(1547072..1547530) 1 NC_000962.3 Rv1374c, (MTCY02B12.08c), len: 152 aa. Hypothetical unknown protein.; Hypothetical protein 1547530 886783 Rv1374c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215890.2 1547072 R 83332 CDS NP_215891.1 15608515 886778 1547832..1549151 1 NC_000962.3 Rv1375, (MTCY02B12.09), len: 439 aa. Conserved hypothetical protein, similar to hypothetical proteins from several organisms e.g. Q52871|U39409 Rhizobium leguminosarum (420 aa), FASTA scores: E(): 2e-30, (34.4% identity in 378 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 1549151 886778 Rv1375 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215891.1 1547832 D 83332 CDS NP_215892.1 15608516 886777 1549148..1550641 1 NC_000962.3 Rv1376, (MTCY02B12.10), len: 497 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from several organisms e.g. Q52872|U39409 Rhizobium leguminosarum (247 aa), FASTA scores: E(): 2.1e-12, (34.7% identity in 219 aa overlap).; hypothetical protein 1550641 886777 Rv1376 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215892.1 1549148 D 83332 CDS NP_215893.1 15608517 886775 complement(1550579..1551217) 1 NC_000962.3 Rv1377c, (MTCY02B12.11c), len: 212 aa. Putative transferase, similar to YQEM_BACSU|P54458 hypothetical 28.3 kDa protein from Bacillus subtilis (247 aa), FASTA scores: opt: 221, E(): 7.6e-08, (30.6% identity in 144 aa overlap); some similarity to methyltransferases, also similar to Mycobacterium tuberculosis hypothetical proteins Rv0560c,Rv3699, and Rv2675c (~ 39.1% identity in 197 aa overlap).; Putative transferase 1551217 886775 Rv1377c Mycobacterium tuberculosis H37Rv Putative transferase NP_215893.1 1550579 R 83332 CDS NP_215894.1 15608518 886773 complement(1551228..1552655) 1 NC_000962.3 Rv1378c, (MTCY02B12.12c), len: 475 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv3074|MTCY22D7.07C (424 aa), FASTA scores: E(): 0, (73.0% identity in 429 aa overlap).; hypothetical protein 1552655 886773 Rv1378c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215894.1 1551228 R 83332 CDS NP_215895.1 15608519 886769 1552654..1553235 1 NC_000962.3 Rv1379, (MTCY02B12.13), len: 193 aa. Probable pyrR,pyrimidine operon regulatory protein, similar to PYRR_BACCL|P41007 pyrimidine operon regulatory protein from Bacillus caldolyticus (179 aa), FASTA scores: opt: 544,E(): 1.1e-30, (54.2% identity in 179 aa overlap).; Probable pyrimidine operon regulatory protein PyrR 1553235 pyrR 886769 pyrR Mycobacterium tuberculosis H37Rv Probable pyrimidine operon regulatory protein PyrR NP_215895.1 1552654 D 83332 CDS NP_215896.1 15608520 886771 1553232..1554191 1 NC_000962.3 Rv1380, (MTCY02B12.14), len: 319 aa. Probable pyrB,aspartate carbamoyltransferase, similar to many e.g. PYRB_BACCL|P41008 aspartate carbamoyltransferase from Bacillus caldolyticus (308 aa), FASTA scores, opt: 639,E(): 7.3e-36, (39.5% identity in 311 aa overlap). Contains PS00097 Aspartate and ornithine carbamoyltransferases signature. Belongs to the ATCases/OTCases family.; Probable aspartate carbamoyltransferase PyrB (ATCase) (aspartate transcarbamylase) 1554191 pyrB 886771 pyrB Mycobacterium tuberculosis H37Rv Probable aspartate carbamoyltransferase PyrB (ATCase) (aspartate transcarbamylase) NP_215896.1 1553232 D 83332 CDS NP_215897.1 15608521 886765 1554188..1555480 1 NC_000962.3 Rv1381, (MTCY02B12.15), len: 430 aa. Probable pyrC,dihydroorotase, similar to many e.g. PYRC_BACCL|P46538 (40.5% identity in 395 aa overlap). Contains PS00483 Dihydroorotase signature 2. Belongs to the DHOase family. subfamily 2.; Probable dihydroorotase PyrC (DHOase) 1555480 pyrC 886765 pyrC Mycobacterium tuberculosis H37Rv Probable dihydroorotase PyrC (DHOase) NP_215897.1 1554188 D 83332 CDS NP_215898.1 15608522 886767 1555477..1555974 1 NC_000962.3 Rv1382, (MTCY02B12.16), len: 165 aa. Possible exported or membrane protein, hydrophobic domain at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable export or membrane protein 1555974 886767 Rv1382 Mycobacterium tuberculosis H37Rv Probable export or membrane protein NP_215898.1 1555477 D 83332 CDS NP_215899.1 15608523 886761 1555971..1557101 1 NC_000962.3 Rv1383, (MTCY02B12.17), len: 376 aa. Probable carA,Carbamoyl-phosphate synthase small chain, similar to many e.g. CARA_ECOLI|P00907 carbamoyl-phosphate synthase small chain from Escherichia coli (382 aa), FASTA scores: opt: 796, E(): 0, (45.5% identity in 382 aa overlap). Contains PS00442 Glutamine amidotransferases class-I active site. The gatase domain belongs to type-1 glutamine amidotransferases. subunit: composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.; Probable carbamoyl-phosphate synthase small chain CarA (carbamoyl-phosphate synthetase glutamine chain) 1557101 carA 886761 carA Mycobacterium tuberculosis H37Rv Probable carbamoyl-phosphate synthase small chain CarA (carbamoyl-phosphate synthetase glutamine chain) NP_215899.1 1555971 D 83332 CDS YP_177804.1 57116856 886253 1557101..1560448 1 NC_000962.3 Rv1384, (MTCY02B12.18-MTCY21B4.01), len: 1115 aa. Probable carB, Carbamoyl-phosphate synthase large chain ,similar to many e.g. CARB_ECOLI|P00968 E. coli (1072 aa),FASTA scores: E(): 0, (52.3% identity in 1118 aa overlap). Contains two PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00866 Carbamoyl-phosphatesynthase subdomain signature 1. subunit: composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.; Probable carbamoyl-phosphate synthase large chain CarB (carbamoyl-phosphate synthetase ammonia chain) 1560448 carB 886253 carB Mycobacterium tuberculosis H37Rv Probable carbamoyl-phosphate synthase large chain CarB (carbamoyl-phosphate synthetase ammonia chain) YP_177804.1 1557101 D 83332 CDS NP_215901.1 15608524 886763 1560445..1561269 1 NC_000962.3 Rv1385, (MTCY21B4.02), len: 274 aa. Probable pyrF,orotidine 5'-phosphate decarboxylase, identical to DCOP_MYCBO|P42610 Mycobacterium bovis (274 aa). Contains PS00156 Orotidine 5'-phosphate decarboxylase active site. Belongs to the OMP decarboxylase family.; Probable orotidine 5'-phosphate decarboxylase PyrF (OMP decarboxylase) (ompdecase) 1561269 pyrF 886763 pyrF Mycobacterium tuberculosis H37Rv Probable orotidine 5'-phosphate decarboxylase PyrF (OMP decarboxylase) (ompdecase) NP_215901.1 1560445 D 83332 CDS YP_177805.1 57116857 886757 1561464..1561772 1 NC_000962.3 Rv1386, (MTCY21B4.03), len: 102 aa. PE15, Member of Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to many e.g. G913039 ORF 3' of PGRS tandem repeat (polymorphic GC-rich sequence) (100 aa), FASTA scores: opt: 149, E(): 0.0013, (31.5% identity in 92 aa overlap); also similar to Q49943|U1756A (99 aa) (34.7% identity in 95 aa overlap) and G466937|U1620K (100 aa) (36.2% identity in 69 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; PE family protein PE15 1561772 PE15 886757 PE15 Mycobacterium tuberculosis H37Rv PE family protein PE15 YP_177805.1 1561464 D 83332 CDS YP_177806.1 57116858 886784 1561769..1563388 1 NC_000962.3 Rv1387, (MTCY21B4.04), len: 539 aa. PPE20, Member of Mycobacterium tuberculosis PPE family of proteins, similar to many e.g. Y05F_MYCTU|Q10892 hypothetical 46.9 kd protein cy251.15 (463 aa), FASTA scores: E(): 4.2e-26, (37.7% identity in 531 aa overlap); similar also to MTCY274.23c (37.5% identity in 168 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; PPE family protein PPE20 1563388 PPE20 886784 PPE20 Mycobacterium tuberculosis H37Rv PPE family protein PPE20 YP_177806.1 1561769 D 83332 CDS NP_215904.1 15608527 886751 1563694..1564266 1 NC_000962.3 Rv1388, (MTCY21B4.05), len: 190 aa. Putative mihF,integration host factor. Almost identical to, but longer than, P96802|U75344 Mycobacterium smegmatis integration host factor (mIHF) for mycobacteriophage L5 (105 aa), FASTA scores: E(): 0, (96.1% identity in 102 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Putative integration host factor MihF 1564266 mihF 886751 mihF Mycobacterium tuberculosis H37Rv Putative integration host factor MihF NP_215904.1 1563694 D 83332 CDS NP_215905.1 15608528 886787 1564401..1565027 1 NC_000962.3 Rv1389, (MTCY21B4.06), len: 208 aa. Probable gmk,guanylate kinase, similar to e.g. KGUA_ECOLI|P24234 guanylate kinase from Escherichia coli (207 aa), FASTA scores: opt: 424, E(): 6.6e-20, (35.9% identity in 184 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00856 Guanylate kinase signature. Belongs to the guanylate kinase family.; Probable guanylate kinase Gmk 1565027 gmk 886787 gmk Mycobacterium tuberculosis H37Rv Probable guanylate kinase Gmk NP_215905.1 1564401 D 83332 CDS NP_215906.1 15608529 886754 1565093..1565425 1 NC_000962.3 Rv1390, (MTCY21B4.07), len: 110 aa. Probable rpoZ,DNA-directed RNA polymerase omega chain. Belongs to the RNA polymerase omega chain family.; Probable DNA-directed RNA polymerase (omega chain) RpoZ (transcriptase omega chain) (RNA polymerase omega subunit) 1565425 rpoZ 886754 rpoZ Mycobacterium tuberculosis H37Rv Probable DNA-directed RNA polymerase (omega chain) RpoZ (transcriptase omega chain) (RNA polymerase omega subunit) NP_215906.1 1565093 D 83332 CDS NP_215907.1 15608530 886749 1565441..1566697 1 NC_000962.3 Rv1391, (MTCY21B4.08), len: 418 aa. Probable dfp,DNA/pantothenate metabolism flavoprotein homolog, similar to many e.g. DFP_ECOLI|P24285 Escherichia coli (430 aa),FASTA scores: opt: 763, E(): 0, (40.2% identity in 408 aa overlap).; Probable DNA/pantothenate metabolism flavoprotein homolog Dfp 1566697 dfp 886749 dfp Mycobacterium tuberculosis H37Rv Probable DNA/pantothenate metabolism flavoprotein homolog Dfp NP_215907.1 1565441 D 83332 CDS NP_215908.1 15608531 886741 1566825..1568036 1 NC_000962.3 Rv1392, (MTCY21B4.09), len: 403 aa. Probable metK,S-adenosylmethionine synthetase, similar to many e.g. METK_STAAU|P50307 Staphylococcus aureus (397 aa), FASTA scores: opt: 1484, E(): 0, (58.0% identity in 400 aa overlap). Contains PS00376 S-adenosylmethionine synthetase signature 1, PS00377 S-adenosylmethionine synthetase signature 2. Belongs to the adomet synthetase family.; Probable S-adenosylmethionine synthetase MetK (mat) (AdoMet synthetase) (methionine adenosyltransferase) 1568036 metK 886741 metK Mycobacterium tuberculosis H37Rv Probable S-adenosylmethionine synthetase MetK (mat) (AdoMet synthetase) (methionine adenosyltransferase) NP_215908.1 1566825 D 83332 CDS NP_215909.1 15608532 886743 complement(1568109..1569587) 1 NC_000962.3 Rv1393c, (MTCY21B4.10c), len: 492 aa. Probable monooxygenase, similar to others e.g. CYMO_ACISP|P12015 cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: E(): 0, (33.0% identity in 473 aa overlap); also to Rv3083|MTCY31.20|E241788 hypothetical 55.0 kDa protein from Mycobacterium tuberculosis (495 aa) (36.3% identity in 490 aa overlap); and Rv0565c, Rv3854c,Rv3049c, Rv0892.; Probable monoxygenase 1569587 886743 Rv1393c Mycobacterium tuberculosis H37Rv Probable monoxygenase NP_215909.1 1568109 R 83332 CDS YP_177807.1 57116859 886738 complement(1569584..1570969) 1 NC_000962.3 Rv1394c, (MT1439, MTCY21B4.11c), len: 461 aa. Probable cyp132, cytochrome P450 132. Some similarity to others e.g. CP4B_HUMAN|P13584 human cytochrome p450 (511 aa), FASTA scores: opt: 486, E(): 7.4e-21, (28.6% identity in 423 aa overlap); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. May belong to the cytochrome P450 family. Experimentally shown that the expression of cyp132 is induced by the transcriptional regulatory protein Rv1395 (Recchi et al., 2003).; Probable cytochrome P450 132 Cyp132 1570969 cyp132 886738 cyp132 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 132 Cyp132 YP_177807.1 1569584 R 83332 CDS YP_177808.1 57116860 886251 1571047..1572081 1 NC_000962.3 Rv1395, (MTCY21B4.12), len: 344 aa. Transcriptional regulatory protein (see citation below), similar to many e.g. URER_PROMI|Q02458 urease operon transcriptional activator from Proteus mirabilis (293 aa), FASTA scores: E():1.5e-08, (41.7% identity in 84 aa overlap); YHIX_ECOLI|P37639 hypothetical transcriptional regulatory protein from Escherichia coli (274 aa), FASTA scores: opt: 238, E(): 3.5e-09, (27.3% identity in 249 aa overlap); and G296916|X68281 possible virulence-regulating protein from Mycobacterium tuberculosis (339 aa), FASTA scores: opt: 228, E(): 1.9e-08, (27.0% identity in 278 aa overlap). Helix turn helix motif present, aa 261-282 (+4.68 SD). Belongs to the AraC/XylS family of transcriptional regulators. 3' part corrected since first submission (-14 aa). Experimentally shown to induce the expression of the cytochrome P450 gene (Rv1394c) and represses its own transcription.; Transcriptional regulatory protein 1572081 886251 Rv1395 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein YP_177808.1 1571047 D 83332 CDS YP_177809.1 57116861 886745 complement(1572127..1573857) 1 NC_000962.3 Rv1396c, (MTCY21B4.13c), len: 576 aa. PE_PGRS25,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002),strong similarity to many e.g. glycine rich protein MTCY130.10C|E245019 (603 aa), FASTA scores: opt: 1945, E(): 0, (57.5% identity in 619 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, similar to other PGRS-type sequences. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE-PGRS family protein PE_PGRS25 1573857 PE_PGRS25 886745 PE_PGRS25 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS25 YP_177809.1 1572127 R 83332 CDS NP_215913.1 15608535 886736 complement(1574112..1574513) 1 NC_000962.3 Rv1397c, (MTCY21B4.14c), len: 133 aa. Possible vapC10, toxin, part of toxin-antitoxin (TA) operon with Rv1398c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Conserved hypothetical protein,similar to Mycobacterium tuberculosis protein MTCY159.08C|Rv2548 (125 aa), FASTA scores: E(): 2.3e-14,(42.4% identity in 125 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC10 1574513 vapC10 886736 vapC10 Mycobacterium tuberculosis H37Rv Possible toxin VapC10 NP_215913.1 1574112 R 83332 CDS NP_215914.1 15608536 886759 complement(1574510..1574767) 1 NC_000962.3 Rv1398c, (MTCY21B4.15c), len: 85 aa. Possible vapB10, antitoxin, part of toxin-antitoxin (TA) operon with Rv1397c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2547|MTCY159.09C (85 aa), FASTA scores: E(): 0.0035,(37.1% identity in 62 aa overlap); Rv0581, Rv2871, Rv1241,etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible antitoxin VapB10 1574767 vapB10 886759 vapB10 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB10 NP_215914.1 1574510 R 83332 CDS NP_215915.1 15608537 886731 complement(1574850..1575809) 1 NC_000962.3 Rv1399c, (MTCY21B4.16c), len: 319 aa. Possible nlhH,non lipolytic carboxylesterase, most similar to G695278 lipase like enzyme from Ralstonia eutropha (364 aa), FASTA scores: opt: 648, E(): 4.4e-34, (37.3% identity in 327 aa ov erlap), similar to Mycobacterium tuberculosis hypothetical lipases e.g. Rv2284, Rv2485c, Rv1426c, etc. Previously known as lipH.; Probable non lipolytic carboxylesterase NlhH 1575809 nlhH 886731 nlhH Mycobacterium tuberculosis H37Rv Probable non lipolytic carboxylesterase NlhH NP_215915.1 1574850 R 83332 CDS NP_215916.1 15608538 886728 complement(1575834..1576796) 1 NC_000962.3 Rv1400c, (MTCY21B4.17c), len: 320 aa. Possible lipI,lipase, most similar to G695278 lipase like enzyme (364 aa), FASTA sscores: opt: 611, E(): 3.5e-30, (36.6% identity in 352 aa overlap); similar to M. tuberculosis hypothetical lipases e.g. Rv1399c|MTCY21B4.16c (58.1% identical in 315 aa overlap); Rv1426c, Rv2284, etc.; Probable lipase LipH 1576796 lipI 886728 lipI Mycobacterium tuberculosis H37Rv Probable lipase LipH NP_215916.1 1575834 R 83332 CDS NP_215917.1 15608539 886733 1576930..1577532 1 NC_000962.3 Rv1401, (MTCY21B4.18), len: 200 aa. Possible membrane protein.; Possible membrane protein 1577532 886733 Rv1401 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215917.1 1576930 D 83332 CDS NP_215918.1 15608540 886716 1577613..1579580 1 NC_000962.3 Rv1402, (MTCY21B4.19), len: 655 aa. Putative priA,primosomal protein N'. Similar to e.g. PRIA_ECOLI|P17888 primosomal protein N' (replication factor Y) (732 aa),FASTA scores, opt: 386, E(): 1.3e-16, (27.6% identity in 711 aa overlap). Compared to other bacterial priA, it has a very divergent helicase domain. Belongs to the helicase family. PRIA subfamily.; Putative primosomal protein N' PriA (replication factor Y) 1579580 priA 886716 priA Mycobacterium tuberculosis H37Rv Putative primosomal protein N' PriA (replication factor Y) NP_215918.1 1577613 D 83332 CDS NP_215919.1 15608541 886717 complement(1579598..1580422) 1 NC_000962.3 Rv1403c, (MTCY21B4.20c), len: 274 aa. Putative methyltransferase, similar to PMTA_RHOSH|Q05197 phosphatidylethanolamine m-methyltransferase (203 aa),FASTA scores: opt: 217, E(): 1.1e-07, (37.1% identity in 105 aa overlap); similar to Rv1405c|MTCY21B4.22c (59.3% identity in 273 aa overlap) and to Rv1523, Rv2952, etc.; Putative methyltransferase 1580422 886717 Rv1403c Mycobacterium tuberculosis H37Rv Putative methyltransferase NP_215919.1 1579598 R 83332 CDS NP_215920.1 15608542 886712 1580591..1581073 1 NC_000962.3 Rv1404, (MTCY21B4.21), len: 160 aa. Probable transcriptional regulatory protein, some similarity to MARR_ECOLI|P27245 multiple antibiotic resistance protein from Escherichia coli (125 aa), FASTA scores: opt: 136,E(): 0.004, (35.1% identity in 74 aa overlap).; Probable transcriptional regulatory protein 1581073 886712 Rv1404 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215920.1 1580591 D 83332 CDS NP_215921.1 15608543 886714 complement(1581145..1581969) 1 NC_000962.3 Rv1405c, (MTCY21B4.22c), len: 274 aa. Putative methyltransferase, most similar to PMTA_RHOSH|Q05197 phosphatidylethanolamine m-methyltransferase (203 aa),FASTA scores: opt: 219, E(): 2.6e-07, (29.9% identity in 144 aa overlap); similar to Rv1403c|MTCY21B4.20c (59.3% identity in 273 aa overlap), Rv1523, Rv2952, etc.; Putative methyltransferase 1581969 886714 Rv1405c Mycobacterium tuberculosis H37Rv Putative methyltransferase NP_215921.1 1581145 R 83332 CDS NP_215922.1 15608544 886706 1582166..1583104 1 NC_000962.3 Rv1406, (MTCY21B4.23), len: 312 aa. Probable fmt,methionyl-tRNA formyltransferase, similar to many e.g. FMT_ECOLI|P23882 Escherichia coli (314 aa), FASTA scores: opt: 616, E(): 6.7e-31, (39.3% identity in 303 aa overlap). Belongs to the FMT family.; Probable methionyl-tRNA formyltransferase Fmt 1583104 fmt 886706 fmt Mycobacterium tuberculosis H37Rv Probable methionyl-tRNA formyltransferase Fmt NP_215922.1 1582166 D 83332 CDS NP_215923.1 15608545 886720 1583101..1584474 1 NC_000962.3 Rv1407, (MTCY21B4.24), len: 457 aa. Probable fmu protein, similar to SUN_ECOLI|P36929 sun protein (fmu protein) from Escherichia coli (429 aa), FASTA scores: E(): 2.5e-20, (30.6% identity in 451 aa overlap).; Probable Fmu protein (sun protein) 1584474 fmu 886720 fmu Mycobacterium tuberculosis H37Rv Probable Fmu protein (sun protein) NP_215923.1 1583101 D 83332 CDS NP_215924.1 15608546 886702 1584499..1585197 1 NC_000962.3 Rv1408, (MTCY21B4.25), len: 232 aa. Probable rpe,ribulose-phosphate 3-epimerase, similar to many e.g. CXEC_ALCEU|P40117 (241 aa), FASTA scores: opt: 638, E(): 1.5e-34, (48.3% identity in 234 aa overlap); and RPE_ECOLI|P32661 ribulose-phosphate 3-epimerase (225 aa),FASTA scores: E(): 0, (46.2% identity in 221 aa overlap). Contains PS01085 Ribulose-phosphate 3-epimerase family signature 1. Belongs to the ribulose-phosphate 3-epimerase family.; Probable ribulose-phosphate 3-epimerase Rpe (PPE) (R5P3E) (pentose-5-phosphate 3-epimerase) 1585197 rpe 886702 rpe Mycobacterium tuberculosis H37Rv Probable ribulose-phosphate 3-epimerase Rpe (PPE) (R5P3E) (pentose-5-phosphate 3-epimerase) NP_215924.1 1584499 D 83332 CDS NP_215925.1 15608547 886721 1585194..1586213 1 NC_000962.3 Rv1409, (MTCY21B4.26), len: 339 aa. Probable ribG (alternate gene name: ribD), bifunctional riboflavin biosynthesis protein, including diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase, similar to many e.g. RIBD_ECOLI|P25539 riboflavin-specific deaminase from Escherichia coli (367 aa), FASTA scores: E(): 0, (39.8% identity in 364 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminases family. In the C-terminal section; belongs to the HTP reductase family.; Probable bifunctional riboflavin biosynthesis protein RibG : diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino) uracil reductase (HTP reductase) 1586213 ribG 886721 ribG Mycobacterium tuberculosis H37Rv Probable bifunctional riboflavin biosynthesis protein RibG : diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino) uracil reductase (HTP reductase) NP_215925.1 1585194 D 83332 CDS NP_215926.1 15608548 886709 complement(1586210..1587766) 1 NC_000962.3 Rv1410c, (MTCY21B4.27c), len: 518 aa. Aminoglycoside/tetracycline-transport integral membrane protein (see citation below), member of major facilitator superfamily (MFS), similar to others e.g. AC22_STRCO|P46105 probable actinorhodin transporter from Streptomyces coelicolor (578 aa), FASTA scores: opt: 442, E(): 4.9e-21,(28.5% identity in 466 aa overlap); etc. Contains PS00216 Sugar transport proteins signature 1. Could be termed P55. Note that the Rv1410c-Rv1411c operon seems transcribed from two promoters in Mycobacterium bovis BCG (see Bigi et al.,2000).; Aminoglycosides/tetracycline-transport integral membrane protein 1587766 886709 Rv1410c Mycobacterium tuberculosis H37Rv Aminoglycosides/tetracycline-transport integral membrane protein NP_215926.1 1586210 R 83332 CDS NP_215927.1 15608549 886700 complement(1587772..1588482) 1 NC_000962.3 Rv1411c, (MTCY21B4.28c), len: 236 aa. lprG (alternate gene name: P27), conserved lipoprotein, similar to Mycobacterium tuberculosis hypothetical lipoproteins e.g. Rv1270c|MTCY50.12 (35.1% identity in 245 aa overlap); Rv1368, Rv2945c. Contains N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Note that the Rv1410c-Rv1411c operon seems transcribed from two promoters in Mycobacterium bovis BCG (see Bigi et al., 2000). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Conserved lipoprotein LprG 1588482 lprG 886700 lprG Mycobacterium tuberculosis H37Rv Conserved lipoprotein LprG NP_215927.1 1587772 R 83332 CDS NP_215928.1 15608550 886690 1588567..1589172 1 NC_000962.3 Rv1412, (MTCY21B4.29), len: 201 aa. Probable ribC (ribE), Riboflavin synthase alpha chain, strong similarity to e.g. RISA_ACTPL|P50854 (215 aa), FASTA scores: opt: 586,E(): 1.8e-33, (50.8% identity in 197 aa overlap). Contains 2 x PS00693 Riboflavin synthase alpha chain family signature.; Probable riboflavin synthase alpha chain RibC (RibE) 1589172 ribC 886690 ribC Mycobacterium tuberculosis H37Rv Probable riboflavin synthase alpha chain RibC (RibE) NP_215928.1 1588567 D 83332 CDS NP_215929.1 15608551 886692 1589386..1589901 1 NC_000962.3 Rv1413, (MTCY21B4.30), len: 171 aa. Conserved hypothetical protein, similar to part of AB010956|AB010956_1 metal-activated pyridoxal enzyme from Arthrobacter sp. (379 aa), FASTA scores: opt: 187, E(): 0.00026, (29.0% identity in 162 aa overlap).; hypothetical protein 1589901 886692 Rv1413 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215929.1 1589386 D 83332 CDS NP_215930.1 15608552 886696 1589891..1590292 1 NC_000962.3 Rv1414, (MTCY21B4.31), len: 133 aa. Conserved hypothetical protein, similar to C-terminal part of AB010956|AB010956_1 novel metal-activated pyridoxal enzyme from Arthrobacter sp. (379 aa), FASTA scores: opt: 163,E(): 0.00063, (32.1% identity in 112 aa overlap). Rv1413 is similar to N-terminal part of same enzyme suggesting possible frameshift. Sequence has been checked and no errors found, it is identical in Mycobacterium bovis strain AF2122/97 and in Mycobacterium tuberculosis CDC1551.; hypothetical protein 1590292 886696 Rv1414 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215930.1 1589891 D 83332 CDS NP_215931.1 15608553 886694 1590397..1591674 1 NC_000962.3 Rv1415, (MTCY21B4.33), len: 425 aa. Probable ribA2,Riboflavin biosynthesis protein, similar to many e.g. GCH2_BACSU|P17620 Bacillus subtilis (398 aa), FASTA scores: opt: 1388, E(): 0, (55.4% identity in 399 aa overlap). Also similar to second Mycobacterium tuberculosis gtp cyclohydrolase Rv1940|ribA1 (353 aa). In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.; Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) 1591674 ribA2 886694 ribA2 Mycobacterium tuberculosis H37Rv Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) NP_215931.1 1590397 D 83332 CDS NP_215932.2 448824757 886681 1591671..1592153 1 NC_000962.3 Rv1416, (MTCY21B4.34), len: 160 aa. Probable ribH,riboflavin synthase beta chain, similar to many e.g. RISB_ECOLI|P25540 Escherichia coli (156 aa), FASTA scores: opt: 330, E(): 1.8e-15, (44.1% identity in 145 aa overlap). Note alternative GTG start possible overlapping the stop codon of Rv1415|MTCY21B4.33. Belongs to the DMRL synthase family. N-terminus extended since first submission (previously 154 aa).; Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase) 1592153 ribH 886681 ribH Mycobacterium tuberculosis H37Rv Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase) NP_215932.2 1591671 D 83332 CDS NP_215933.1 15608555 886704 1592150..1592614 1 NC_000962.3 Rv1417, (MTCY21B4.35), len: 154 aa. Possible conserved membrane protein, similar to others e.g. AL133213|SC6D7_2 Streptomyces coelicolor (156 aa), FASTA scores: opt: 212, E(): 4.4e-07, (32.4% identity in 136 aa overlap).; Possible conserved membrane protein 1592614 886704 Rv1417 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_215933.1 1592150 D 83332 CDS NP_215934.1 15608556 886687 1592639..1593325 1 NC_000962.3 Rv1418, (MTCY21B4.36), len: 228 aa. Probable lprH,lipoprotein. Contains N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013).; Probable lipoprotein LprH 1593325 lprH 886687 lprH Mycobacterium tuberculosis H37Rv Probable lipoprotein LprH NP_215934.1 1592639 D 83332 CDS NP_215935.1 15608557 886683 1593505..1593978 1 NC_000962.3 Rv1419, (MTCY21B4.37), len: 157 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 1593978 886683 Rv1419 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215935.1 1593505 D 83332 CDS NP_215936.1 15608558 886672 1594042..1595982 1 NC_000962.3 Rv1420, (MTCY21B4.38), len: 646 aa. Probable uvrC,excinuclease ABC, subunit C; nuclease (see citations below), similar to many e.g. UVRC_PSEFL|P32966 Pseudomonas fluorescens (607 aa), fasta scores: opt: 738, E(): 8.4e-39,(36.6% identity in 629 aa overlap). Belongs to the UvrC family.; Probable excinuclease ABC (subunit C-nuclease) UvrC 1595982 uvrC 886672 uvrC Mycobacterium tuberculosis H37Rv Probable excinuclease ABC (subunit C-nuclease) UvrC NP_215936.1 1594042 D 83332 CDS NP_215937.1 15608559 886676 1595979..1596884 1 NC_000962.3 Rv1421, (MTCY21B4.39), len: 301 aa. Conserved protein, similar to many hypothetical proteins e.g. YHBJ_ECOLI|P33995 hypothetical 32.5 kd protein from Escherichia coli (284 aa), FASTA scores: opt: 648, E(): 6.3e-36, (38.7% identity in 282aa overlap).; hypothetical protein 1596884 886676 Rv1421 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215937.1 1595979 D 83332 CDS NP_215938.1 15608560 886670 1596881..1597909 1 NC_000962.3 Rv1422, (MTCY21B4.40), len: 342 aa. Conserved hypothetical protein, similar to many hypothetical proteins e.g. YAMB_THETU|P38541 Thermoanaerobacterium thermosulfurigenes (323 aa), FASTA scores: opt: 519, E(): 1.6e-25, (33.1% identity in 320 aa overlap); and AF106003|AF106003_3 Streptomyces coelicolor (363 aa), FASTA scores: opt: 1047, E(): 0, (54.5% identity in 308 aa overlap).; hypothetical protein 1597909 886670 Rv1422 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215938.1 1596881 D 83332 CDS NP_215939.1 15608561 886674 1597906..1598883 1 NC_000962.3 Rv1423, (MTCY21B4.41-MTCY493.31c), len: 325 aa. Putative whiA, transcriptional regulator, probably equivalent to AL035591|SCC54.10 whiA protein from Streptomyces coelicolor (328 aa), FASTA scores: opt: 1505,E(): 0, (70.4% identity in 324 aa overlap). Also some similarity to O06975|YVCL hypothetical protein from Bacillus subtilis (316 aa), FASTA scores: E(): 1.8e-0 8,(25.7% identity in 304 aa overlap).; Probable transcriptional regulatory protein WhiA 1598883 whiA 886674 whiA Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein WhiA NP_215939.1 1597906 D 83332 CDS NP_215940.1 15608562 886685 complement(1598893..1599654) 1 NC_000962.3 Rv1424c, (MTCY21B4.42c,MTCY493.30), len: 253 aa. Possible membrane protein, contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible membrane protein 1599654 886685 Rv1424c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215940.1 1598893 R 83332 CDS NP_215941.1 15608563 886668 1599658..1601037 1 NC_000962.3 Rv1425, (MTCY21B4.43,MTCY493.29c), len: 459 aa. Possible triacylglycerol synthase (See Daniel et al.,2004), similar to many M. tuberculosis proteins e.g. Rv3740c, Rv3734c, Rv1760, etc.; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 1601037 886668 Rv1425 Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_215941.1 1599658 D 83332 CDS NP_215942.1 15608564 886660 complement(1601059..1602321) 1 NC_000962.3 Rv1426c, (MTCY493.28), len: 420 aa. Possible lipO,esterase, similar to several Mycobacterium tuberculosis hypothetical lipases and esterases e.g. Rv1399c, Rv2284,etc. Also similar in central region to AAAD_HUMAN|P22760 human arylacetamide deacetylase (398 aa), FASTA scores: opt:210, E(): 7.6e-07, (29.3% identity in 191 aa overlap).; Probable esterase LipO 1602321 lipO 886660 lipO Mycobacterium tuberculosis H37Rv Probable esterase LipO NP_215942.1 1601059 R 83332 CDS NP_215943.1 15608565 886679 complement(1602321..1603928) 1 NC_000962.3 Rv1427c, (MTCY493.27), len: 535 aa. Possible fadD12,long-chain-fatty-acid-CoA synthetase, similar to many e.g. NP_302632.1|NC_002677 acyl-CoA synthase from Mycobacterium leprae (548 aa); AAD01929.2|AF031419 putative long-chain-fatty-acid--CoA ligase from Pseudomonas putida (565 aa); NP_419782.1|NC_002696 putative long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (530 aa); PC60_YEAST|P38137 yeast peroxisomal-coenzyme A synthetase (543 aa), FASTA scores: opt: 507, E(): 2.9e-25, (30.4% identity in 365 aa overlap). Also similar to many M. tuberculosis proteins e.g. MTCY06A4.14 (44.8% identity in 525 aa overlap). Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Possible long-chain-fatty-acid--CoA ligase FadD12 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 1603928 fadD12 886679 fadD12 Mycobacterium tuberculosis H37Rv Possible long-chain-fatty-acid--CoA ligase FadD12 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_215943.1 1602321 R 83332 CDS NP_215944.1 15608566 886656 complement(1603932..1604759) 1 NC_000962.3 Rv1428c, (MTCY493.26), len: 275 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0502|YV29_MYCTU|Q11167 (358 aa), FASTA scores: opt: 355,E(): 5e-16, (32.6% identity in 273 aa overlap); and Rv1920.; hypothetical protein 1604759 886656 Rv1428c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215944.1 1603932 R 83332 CDS NP_215945.1 15608567 886658 1604878..1606146 1 NC_000962.3 Rv1429, (MTCY493.25c), len: 422 aa. Conserved protein, some similarity to transcriptional regulator proteins e.g. CDAR_ECOLI|P37047 Carbohydrate diacid regulator from Escherichia coli (391 aa), FASTA scores: opt: 210, E(): 3e-06, (27.7% identity in 296 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv2370c, Rv1194c, Rv1453, Rv2242, and Rv1186c.; hypothetical protein 1606146 886658 Rv1429 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215945.1 1604878 D 83332 CDS YP_177810.1 57116862 886652 1606386..1607972 1 NC_000962.3 Rv1430, (MTCY493.24c), len: 528 aa. PE16, Member of the Mycobacterium tuberculosis PE family of proteins (see citation below), e.g. Y0D4_MYCTU|Q50594 (55.9% identity in 127 aa overlap). The C-terminus shows similarity to Q49633|LEPB1170_F3_112 hypothetical Mycobacterium leprae protein (391 aa), FASTA scores: opt: 342, E(): 1.2e-13,(29.8% identity in 292 aa overlap). Possible TMhelix aa 500-522.; PE family protein PE16 1607972 PE16 886652 PE16 Mycobacterium tuberculosis H37Rv PE family protein PE16 YP_177810.1 1606386 D 83332 CDS NP_215947.1 15608569 886662 1608083..1609852 1 NC_000962.3 Rv1431, (MTCY493.23c), len: 589 aa. Conserved membrane protein, shows strong similarity to another M. tuberculosis hypothetical protein Rv1132|MTCY22G8.21 (48.2% identity in 585 aa overlap).; hypothetical protein 1609852 886662 Rv1431 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215947.1 1608083 D 83332 CDS NP_215948.1 15608570 886643 1609849..1611270 1 NC_000962.3 Rv1432, (MTCY493.22c), len: 473 aa. Probable dehydrogenase, shows strong similarity to P49_STRLI|P06108 p49 protein from Streptomyces lividans (469 aa), FASTA scores: opt: 1362, E(): 0, (44.9% identity in 474 aa overlap); and weak similarity to other dehydrogenases.; Probable dehydrogenase 1611270 886643 Rv1432 Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_215948.1 1609849 D 83332 CDS NP_215949.1 15608571 886649 1611434..1612249 1 NC_000962.3 Rv1433, (MTCY493.21c), len: 271 aa. Possible exported protein with N-terminal signal sequence, highly similar to Q49706 hypothetical protein from Mycobacterium leprae (271 aa), FASTA scores: opt: 1341, E(): 0, (68.3% identity in 271 aa overlap). Also shows similarity to M. tuberculosis lipoprotein Rv2518c|MTV009.03c lppS (408 aa) (40.0% identity in 230 aa overlap); and others e.g. Rv0116c, Rv0192, Rv2518c, Rv0483. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved exported protein 1612249 886649 Rv1433 Mycobacterium tuberculosis H37Rv Possible conserved exported protein NP_215949.1 1611434 D 83332 CDS NP_215950.1 15608572 886634 1612256..1612393 1 NC_000962.3 Rv1434, (MTCY493.20c), len: 45 aa. Hypothetical unknown protein.; Hypothetical protein 1612393 886634 Rv1434 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_215950.1 1612256 D 83332 CDS NP_215951.1 15608573 886653 complement(1612342..1612950) 1 NC_000962.3 Rv1435c, (MTCY493.19), len: 202 aa. Probable conserved Pro-, Gly-, Val-rich secreted protein (see citation below) with a N-terminal signal sequence. Similar at C-terminus to AF017099|AF017099_1 Mycobacterium tuberculosis pGB1 (87 aa), FASTA scores: opt: 550, E(): 2.3e-17, (97.7% identity in 86 aa overlap). Shows some similarity to N-terminus of CPN_DROME|Q02910 calphotin. Drosophila melanogaster (865 aa), FASTA scores: opt: 266,E(): 2.5e-05, (37.2% identity in 191 aa overlap). Contains at least five 7 aa imperfect repeats. Also shows similarity to other Mycobacterium tuberculosis proteins e.g. MTCI237.20c (34.7% identity in 193 aa overlap), MTCI65.25c (36.9% identity in 160 aa overlap) and MTCI65.24c (34.2% identity in 196 aa overlap).; Probable conserved proline, glycine, valine-rich secreted protein 1612950 886653 Rv1435c Mycobacterium tuberculosis H37Rv Probable conserved proline, glycine, valine-rich secreted protein NP_215951.1 1612342 R 83332 CDS NP_215952.1 15608574 886632 1613307..1614326 1 NC_000962.3 Rv1436, (MTCY493.18c), len: 339 aa. Probable gap,Glyceraldehyde 3-phosphate dehydrogenase, highly similar to many e.g. G3P_MYCLE|P46713 Mycobacterium leprae (339 aa),FASTA scores: opt: 1933, E():0, (89.1% identity in 339 aa overlap). Contains PS00071 Glyceraldehyde 3-phosphate dehydrogenase active site. Belongs to the glyceraldehyde 3-phosphate dehydrogenase family.; Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH) 1614326 gap 886632 gap Mycobacterium tuberculosis H37Rv Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH) NP_215952.1 1613307 D 83332 CDS NP_215953.1 15608575 886636 1614329..1615567 1 NC_000962.3 Rv1437, (MTCY493.17c), len: 412 aa. Probable pgk,Phosphoglycerate kinase, highly similar to many e.g. PGK_MYCLE|P46712 Mycobacterium leprae (416 aa), FASTA scores: opt: 2153, E(): 0, (80.4% identity in 414 aa overlap). Contains PS00111 Phosphoglycerate kinase signature. Belongs to the phosphoglycerate kinase family.; Probable phosphoglycerate kinase Pgk 1615567 pgk 886636 pgk Mycobacterium tuberculosis H37Rv Probable phosphoglycerate kinase Pgk NP_215953.1 1614329 D 83332 CDS NP_215954.1 15608576 886628 1615564..1616349 1 NC_000962.3 Rv1438, (MTCY493.16c), len: 261 aa. Probable tpi (tpiA), Triosephosphate isomerase, highly similar to many e.g. TPIS_MYCLE|P46711 Mycobacterium leprae (261 aa), FASTA scores: opt: 1456, E(): 0, (83.9% identity in 261 aa overlap). Contains PS00171 Triosephosphate isomerase active site. Belongs to the triosephosphate isomerase family.; Probable triosephosphate isomerase Tpi (TIM) 1616349 tpi 886628 tpi Mycobacterium tuberculosis H37Rv Probable triosephosphate isomerase Tpi (TIM) NP_215954.1 1615564 D 83332 CDS NP_215955.1 15608577 886630 complement(1616961..1617386) 1 NC_000962.3 Rv1439c, (MTCY493.15), len: 141 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 1617386 886630 Rv1439c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215955.1 1616961 R 83332 CDS NP_215956.2 57116863 886624 1617837..1618070 1 NC_000962.3 Rv1440, (MTCY493.14c), len: 77 aa. Probable secG,protein-export membrane protein (translocase subunit) (see citation below), similar to many e.g. P38388|SECG_MYCLE probable protein-export membrane (77 aa), FASTA scores: opt: 450, E(): 6.7e-24, (96.1% identity in 77 aa overlap). Start changed since original submission (-40 aa). Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638,SECF|Rv2586c, SECG and SECY|Rv0732.; Probable protein-export membrane protein (translocase subunit) SecG 1618070 secG 886624 secG Mycobacterium tuberculosis H37Rv Probable protein-export membrane protein (translocase subunit) SecG NP_215956.2 1617837 D 83332 CDS YP_177811.1 57116864 886626 complement(1618209..1619684) 1 NC_000962.3 Rv1441c, (MTCY493.13), len: 491 aa. PE_PGRS26,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002),similar to Y0DP_MYCTU|Q50615 hypothetical glycine-rich 40.8 kDa protein (498 aa), fasta scores: opt: 1625, E(): 0,(55.2% identity in 518 aa overlap).; PE-PGRS family protein PE_PGRS26 1619684 PE_PGRS26 886626 PE_PGRS26 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS26 YP_177811.1 1618209 R 83332 CDS NP_215958.1 15608580 886620 1619791..1622091 1 NC_000962.3 Rv1442, (MTCY493.12c), len: 766 aa. Probable bisC,Biotin sulfoxide reductase, similar to BISC_ECOLI|P20099 biotin sulfoxide reductase from Escherichia coli (739 aa),FASTA scores: opt: 1271, E():0, (40.2% identity in 744 aa overlap).; Probable biotin sulfoxide reductase BisC (BDS reductase) (BSO reductase) 1622091 bisC 886620 bisC Mycobacterium tuberculosis H37Rv Probable biotin sulfoxide reductase BisC (BDS reductase) (BSO reductase) NP_215958.1 1619791 D 83332 CDS NP_215959.1 15608581 886622 complement(1622207..1622692) 1 NC_000962.3 Rv1443c, (MTCY493.11), len: 161 aa. Unknown protein.; hypothetical protein 1622692 886622 Rv1443c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215959.1 1622207 R 83332 CDS NP_215960.1 15608582 886616 complement(1623287..1623697) 1 NC_000962.3 Rv1444c, (MTCY493.10), len: 136 aa. Unknown protein.; hypothetical protein 1623697 886616 Rv1444c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215960.1 1623287 R 83332 CDS NP_215961.1 15608583 886617 complement(1623714..1624457) 1 NC_000962.3 Rv1445c, (MTCY493.09), len: 247 aa. Possible devB (PGL), 6-phosphogluconolactonase, belongs to a different family to the upstream gene zwf2. Similar to e.g. DEVB_ANASP|P46016 putative glucose-6-phosphate 1-dehydrogenase (239 aa), FASTA scores: opt: 439, E(): 2.6e-20, (34.0% identity in 247 aa overlap). Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.; Probable 6-phosphogluconolactonase DevB (6PGL) 1624457 devB 886617 devB Mycobacterium tuberculosis H37Rv Probable 6-phosphogluconolactonase DevB (6PGL) NP_215961.1 1623714 R 83332 CDS NP_215962.1 15608584 886612 complement(1624454..1625365) 1 NC_000962.3 Rv1446c, (MTCY493.08), len: 303 aa. Putative opcA,OxPP cycle protein. Highly similar to S72774 B1496_F1_30 protein from Mycobacterium leprae (265 aa), FASTA scores: opt: 1056, E(): 0, (70.3% identity in 239 aa overlap). Also similar to OPCA_NOSS2|P48971 putative oxppcycle protein opca from Nostoc punctiforme (465 aa), fasta scores: opt: 177, E(): 7.3e-05, (23.4% identity in 321 aa overlap). Aids in G6PD activity.; Putative OXPP cycle protein OpcA 1625365 opcA 886612 opcA Mycobacterium tuberculosis H37Rv Putative OXPP cycle protein OpcA NP_215962.1 1624454 R 83332 CDS NP_215963.1 15608585 886614 complement(1625418..1626962) 1 NC_000962.3 Rv1447c, (MTCY493.07), len: 514 aa. Probable zwf2 (ZWF), Glucose-6-phosphate 1-dehydrogenase, highly similar to many e.g. G6PD_SYNY3|P73411 Synechocystis sp. (509 aa),FASTA scores: opt: 1578, E(): 0, (46.8% identity in 509 aa overlap). Also similar to M. tuberculosis Rv1121, zwf glucose-6-phosphate 1-dehydrogenase. Contains PS00069 Glucose-6-phosphate dehydrogenase active site. Mycobacterium tuberculosis has two genes for ZWF. This one looks like a classical ZWF. Belongs to the glucose-6-phosphate dehydrogenase family.; Probable glucose-6-phosphate 1-dehydrogenase Zwf2 (G6PD) 1626962 zwf2 886614 zwf2 Mycobacterium tuberculosis H37Rv Probable glucose-6-phosphate 1-dehydrogenase Zwf2 (G6PD) NP_215963.1 1625418 R 83332 CDS NP_215964.1 15608586 886606 complement(1626959..1628080) 1 NC_000962.3 Rv1448c, (MTCY493.06), len: 373 aa. Probable tal,Transaldolase, highly similar to many e.g. TAL_MYCLE|P55193 transaldolase from Mycobacterium leprae (375 aa), FASTA scores: opt: 1891, E(): 0, (78.6% identity in 370 aa overlap). Belongs to the transaldolase family.; Probable transaldolase Tal 1628080 tal 886606 tal Mycobacterium tuberculosis H37Rv Probable transaldolase Tal NP_215964.1 1626959 R 83332 CDS NP_215965.1 15608587 886638 complement(1628097..1630199) 1 NC_000962.3 Rv1449c, (MTCY493.05), len: 700 aa. tkt,transketolase. Highly similar to several e.g. TKT_MYCLE|P46708 transketolase (tk) from Mycobacterium leprae (699 aa), FASTA scores: opt: 4216, E(): 0, (89.1% identity in 700 aa overlap). Start site chosen by homology. Contains PS00801 Transketolase signature 1. Belongs to the transketolase family. Thought to be differentially expressed within host cells (see Triccas et al., 1999).; Transketolase Tkt (TK) 1630199 tkt 886638 tkt Mycobacterium tuberculosis H37Rv Transketolase Tkt (TK) NP_215965.1 1628097 R 83332 CDS YP_177812.1 57116865 886605 complement(1630638..1634627) 1 NC_000962.3 Rv1450c, (MTCY493.04), len: 1329 aa. PE_PGRS27,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu,2002), similar to Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), fasta scores: opt: 2112, E(): 0, (56.5% identity in 630 aa overlap).; PE-PGRS family protein PE_PGRS27 1634627 PE_PGRS27 886605 PE_PGRS27 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS27 YP_177812.1 1630638 R 83332 CDS NP_215967.1 15608589 886666 1635029..1635955 1 NC_000962.3 Rv1451, (MTCY493.03c), len: 308 aa. Probable ctaB,cytochrome C oxidase assembly factor, and integral membrane protein. Highly similar to several Mycobacterium leprae proteins e.g. Q49685 CYOE cytochrome O ubiquinol oxidase assembly factor (300 aa), FASTA scores: opt: 1636, E(): 0,(82.7% identity in 307 aa overlap); NP_301495.1|NC_002677 putative protoheme IX farnesyltransferase (321 aa); NP_301495.1|NC_002677 putative protoheme IX farnesyltransferase (321 aa).; Probable cytochrome C oxidase assembly factor CtaB 1635955 ctaB 886666 ctaB Mycobacterium tuberculosis H37Rv Probable cytochrome C oxidase assembly factor CtaB NP_215967.1 1635029 D 83332 CDS YP_177813.1 57116866 886595 complement(1636004..1638229) 1 NC_000962.3 Rv1452c, (MTCY493.02), len: 741 aa. PE_PGRS28,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu,2002), similar to Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), fasta scores: opt: 2090, E(): 0, (56.3% identity in 641 aa overlap).; PE-PGRS family protein PE_PGRS28 1638229 PE_PGRS28 886595 PE_PGRS28 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS28 YP_177813.1 1636004 R 83332 CDS NP_215969.2 57116867 886610 1638381..1639646 1 NC_000962.3 Rv1453, (MTCY493.01c), len: 421 aa. Possible transcriptional activator, similar to Q50018 putative transcriptional activator trx from Mycobacterium leprae (517 aa), FASTA scores: opt: 1719, E(): 0, (54.0% identity in 500 aa overlap). Also highly similar to Mycobacterium tuberculosis proteins Rv2370c, Rv1194c, Rv2242, Rv1186c,and to the further upstream ORF's Rv1429|MTCY493.25c (28.1% identity in 335 aa overlap). Start changed since first submission (-11 aa).; Possible transcriptional activator protein 1639646 886610 Rv1453 Mycobacterium tuberculosis H37Rv Possible transcriptional activator protein NP_215969.2 1638381 D 83332 CDS NP_215970.1 15608592 886589 complement(1639674..1640660) 1 NC_000962.3 Rv1454c, (MTV007.01c), len: 328 aa. Probable qor,quinone oxidoreductase, simiar to U87282|RCU87282_2 quinone oxidoreductase from Rhodobacter capsulatus (323 aa), FASTA scores: opt: 849, E(): 0, (44.7% identity in 329 aa overlap). Also similar to MTCY180.06 Hypothetical protein from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 430, E(): 2e-14, (32.3% identity in 350 aa overlap). Contains PS01162 Quinone oxidoreductase / zeta-crystallin signature.; Probable quinone reductase Qor (NADPH:quinone reductase) (zeta-crystallin homolog protein) 1640660 qor 886589 qor Mycobacterium tuberculosis H37Rv Probable quinone reductase Qor (NADPH:quinone reductase) (zeta-crystallin homolog protein) NP_215970.1 1639674 R 83332 CDS NP_215971.1 15608593 886597 1640680..1641543 1 NC_000962.3 Rv1455, (MTV007.02), len: 287 aa. Conserved protein,some similarity from aa 80-160 to Z99125|MLCL536.35c hypothetical Mycobacterium leprae protein (101 aa), FASTA scores: opt: 238, E(): 1.8e-08, (51.3% identity in 78 aa overlap).; hypothetical protein 1641543 886597 Rv1455 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215971.1 1640680 D 83332 CDS NP_215972.1 15608594 886591 complement(1641493..1642425) 1 NC_000962.3 Rv1456c, (MTV007.03c), len: 310 aa. Possible unidentified antibiotic-transport integral membrane protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.34 from Mycobacterium leprae (311 aa), FASTA scores: opt: 1607, E(): 0, (83.3% identity in 300 aa overlap).; Probable unidentified antibiotic-transport integral membrane ABC transporter 1642425 886591 Rv1456c Mycobacterium tuberculosis H37Rv Probable unidentified antibiotic-transport integral membrane ABC transporter NP_215972.1 1641493 R 83332 CDS NP_215973.1 15608595 886587 complement(1642537..1643322) 1 NC_000962.3 Rv1457c, (MTV007.04c), len: 261 aa. Possible unidentified antibiotic-transport integral membrane protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.32 from Mycobacterium leprae (265 aa), FASTA scores: opt: 1415, E(): 0, (83.1% identity in 260 aa overlap).; Probable unidentified antibiotic-transport integral membrane ABC transporter 1643322 886587 Rv1457c Mycobacterium tuberculosis H37Rv Probable unidentified antibiotic-transport integral membrane ABC transporter NP_215973.1 1642537 R 83332 CDS NP_215974.1 15608596 886582 complement(1643319..1644260) 1 NC_000962.3 Rv1458c, (MTV007.05c), len: 313 aa. Possible unidentified antibiotic-transport ATP-binding protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.31 from Mycobacterium leprae (315 aa), FASTA scores: opt: 1812, E(): 0, (88.0% identity in 308 aa overlap). Similar to AF027770|AF027770_7 ABC-type transporter in FxbA region in Mycobacterium smegmatis (284 aa), FASTA scores: opt: 1412, E(): 0, (85.1% identity in 248 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable unidentified antibiotic-transport ATP-binding protein ABC transporter 1644260 886582 Rv1458c Mycobacterium tuberculosis H37Rv Probable unidentified antibiotic-transport ATP-binding protein ABC transporter NP_215974.1 1643319 R 83332 CDS NP_215975.1 15608597 886585 complement(1644363..1646138) 1 NC_000962.3 Rv1459c, (MTV007.06c), len: 591 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Possible conserved integral membrane protein, equivalent to MLCL536.30|Z99125 hypothetical protein from Mycobacterium leprae (593 aa), FASTA scores: opt: 1670, E(): 0, (78.6% identity in 585 aa overlap). Also similar to M. tuberculosis protein Rv2174|MTV021.07 (33.1% identity in 523 aa overlap).; Possible conserved integral membrane protein 1646138 886585 Rv1459c Mycobacterium tuberculosis H37Rv Possible conserved integral membrane protein NP_215975.1 1644363 R 83332 CDS NP_215976.2 57116868 886573 1646186..1646992 1 NC_000962.3 Rv1460, (MTV007.07), len: 268 aa. Probable transcriptional regulatory protein. Equivalent to Z99125|MLCL536.29c hypothetical protein from Mycobacterium leprae (254 aa), FASTA scores: opt: 1273, E(): 0, (79.6% identity in 250 aa overlap). Possible helix-turn-helix motif between aa 68 - 89. Start changed since original submission.; Probable transcriptional regulatory protein 1646992 886573 Rv1460 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215976.2 1646186 D 83332 CDS NP_215977.1 15608599 886609 1646989..1649529 1 NC_000962.3 Rv1461, (MTV007.08), len: 846 aa. Conserved protein. Equivalent of spliced protein from Mycobacterium leprae MLCL536.28c len: 869. Residues 1-253 represent N-extein,and 613-846 the C-extein. The intein present from residues 254 - 612 is different in sequence and site of the insertion from the one present in MLCL536.28c. FASTA scores: Z99125|MLCL536_23 Mycobacterium leprae cosmid L536 (869 aa), opt: 1498 E(): 0, (54.1% identity in 917 aa overlap). The mature protein is similar to Z99120|BSUB0017_150 hypothetical Bacillus subtilis protein (465 aa), FASTA scores: opt:1053, E(): 0, (34.8% identity in 821 aa overlap). The intein shows some similarity to inteins from U67548|MJU67548_6 Methanococcus jannaschii (895 aa), FASTA scores: opt: 181, E(): 0.00023, (25.2% identity in 274 aa overlap).; hypothetical protein 1649529 886609 Rv1461 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215977.1 1646989 D 83332 CDS NP_215978.1 15608600 886567 1649526..1650719 1 NC_000962.3 Rv1462, (MTV007.09), len: 397 aa. Conserved hypothetical protein. Equivalent to MLCL536.27c|Z99125 hypothetical protein from Mycobacterium leprae (392 aa),FASTA scores: opt: 2059, E(): 0, (80.4% identity in 392 aa overlap). Also similar to nearby Mycobacterium tuberculosis hypothetical protein Rv1461.; hypothetical protein 1650719 886567 Rv1462 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215978.1 1649526 D 83332 CDS NP_215979.1 15608601 886571 1650716..1651516 1 NC_000962.3 Rv1463, (MTV007.10), len: 266 aa. Probable conserved ATP-binding protein ABC transporter, equivalent to Z99125|MLCL536.26c putative ABC transporter ATP-binding protein from Mycobacterium leprae (260 aa), FASTA scores: opt: 1444, E(): 0, (86.0% identity in 267 aa overlap). Very similar to U38804|PPU38804_55 ATP-dependent transporter YCF16 from porphyra purpurea chloroplast (251 aa), FASTA scores: opt: 822, E(): 0, (52.4% identity in 248 aa overlap); and similar to others. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable conserved ATP-binding protein ABC transporter 1651516 886571 Rv1463 Mycobacterium tuberculosis H37Rv Probable conserved ATP-binding protein ABC transporter NP_215979.1 1650716 D 83332 CDS NP_215980.1 15608602 886565 1651518..1652771 1 NC_000962.3 Rv1464, (MTV007.11), len: 417 aa. Probable csd,cysteine desulfurase. Equivalent to Q49690|MLCL536.25C cysteine desulfurase from Mycobacterium leprae (418 aa),FASTA scores: opt: 2333, E(): 0, (85.4% identity in 417 aa overlap); and similar to cysteine desulfurase from other organisms. Also similar to M. tuberculosis proteins Rv3025c|ISCS and Rv3778c. Contains PS00595 Aminotransferases class-V pyridoxal-phosphate attachment site. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases. CSD subfamily.; Probable cysteine desulfurase Csd 1652771 csd 886565 csd Mycobacterium tuberculosis H37Rv Probable cysteine desulfurase Csd NP_215980.1 1651518 D 83332 CDS NP_215981.1 15608603 886569 1652768..1653256 1 NC_000962.3 Rv1465, (MTV007.12), len: 162 aa. Possible nitrogen fixation related protein. Equivalent to Z99125|MLCL536.24c nitrogen fixation protein NIFU from Mycobacterium leprae (165 aa), FASTA scores: opt: 870, E(): 0, (81.8% identity in 165 aa overlap). Also similar to O32163|Z99120|NIFU_BACSU NifU-like protein from Bacillus subtilis (147 aa), FASTA scores: opt: 354, E(): 4.1e-17,(38.3% identity in 141 aa overlap) and to AL096839|SCC22.02 hypothetical protein from Streptomyces coelicolor (156 aa),FASTA scores: opt: 569, E(): 1.2e-31, (56.3% identity in 158 aa overlap).; Possible nitrogen fixation related protein 1653256 886569 Rv1465 Mycobacterium tuberculosis H37Rv Possible nitrogen fixation related protein NP_215981.1 1652768 D 83332 CDS NP_215982.1 15608604 886561 1653231..1653578 1 NC_000962.3 Rv1466, (MTV007.13), len: 115 aa. Conserved protein. Equivalent to Z99125|MLCL536.23c hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 648, E(): 0, (81.7% identity in 115 aa overlap). Similar to ORF's downstream of sigma factors in Streptococcus mutans and Streptococcus pneumoniae e.g. O06451 ORF3 downstream of RpoD (SPDNAGCPO) (109 aa). Alternative TTG start possible at 13757 then avoids overlap with MTV007.12.; hypothetical protein 1653578 886561 Rv1466 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215982.1 1653231 D 83332 CDS NP_215983.1 15608605 886562 complement(1653673..1655502) 1 NC_000962.3 Rv1467c, (MTV007.14c), len: 609 aa. Probable fadE15,acyl-CoA dehydrogenase, highly similar to NP_302639.1|NC_002677 acyl-CoA dehydrogenase from Mycobacterium leprae (611 aa). Also highly similar to many e.g. T36481 probable acyl-CoA dehydrogenase (fragment) from Streptomyces coelicolor (491 aa) (has its N-terminus very shorter); NP_384640.1|NC_003047 putative acyl-CoA dehydrogenase protein from Sinorhizobium meliloti (598 aa); ACDS_MEGEL|Q06319 acyl-CoA dehydrogenase (short-chain specific) from Megasphaera elsdenii (383 aa), FASTA scores: E(): 2e-12, (25.4% identity in 410 aa overlap); etc. Also highly similar to fadE5|Rv0244c|MTV034.10c acyl-CoA dehydrogenase from Mycobacterium tuberculosis (611 aa); and similar to other proteins from Mycobacterium tuberculosis.; Probable acyl-CoA dehydrogenase FadE15 1655502 fadE15 886562 fadE15 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE15 NP_215983.1 1653673 R 83332 CDS YP_177814.1 57116869 886556 complement(1655609..1656721) 1 NC_000962.3 Rv1468c, (MTV007.15c), len: 370 aa. PE_PGRS29,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below).; PE-PGRS family protein PE_PGRS29 1656721 PE_PGRS29 886556 PE_PGRS29 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS29 YP_177814.1 1655609 R 83332 CDS NP_215985.1 15608607 886578 1656963..1658936 1 NC_000962.3 Rv1469, (MTV007.16), len: 657 aa. Probable ctpD,cation-transporting P-type ATPase D (transmembrane protein), highly similar to others e.g. T35947 probable cation-transporting ATPase from Streptomyces coelicolor (638 aa); NP_442633.1|NC_000911 cation-transporting ATPase (E1-E2 ATPase) from Synechocystis sp. strain PCC 6803 (642 aa), FASTA scores: opt: 1438, E(): 0, (41.9% identity in 592 aa overlap); NP_389268.1|NC_000964 protein similar to heavy metal-transporting ATPase from Bacillus subtilis (637 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv3743c|MTV025.091c|CTPJ (660 aa). Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB.; Probable cation transporter P-type ATPase D CtpD 1658936 ctpD 886578 ctpD Mycobacterium tuberculosis H37Rv Probable cation transporter P-type ATPase D CtpD NP_215985.1 1656963 D 83332 CDS NP_215986.1 15608608 886558 1658980..1659354 1 NC_000962.3 Rv1470, (MTV007.17), len: 124 aa. Probable trxA,thioredoxin, similar to many e.g. P12243|THI1_SYNP7 thioredoxin 1 from Synechococcus sp. (106 aa), FASTA scores: opt: 201, E(): 9.2e-08, (35.4% identity in 99 aa overlap); etc. Highly similar to downstream ORF Rv1471|trxB1 probable thioredoxin from Mycobacterium tuberculosis (123 aa), FASTA scores: opt: 402, E(): 0,(54.4% identity in 114 aa overlap). Warning: note that Rv3914|MT4033|MTV028.05|trxC can be alternatively named trxA.; Probable thioredoxin TrxA 1659354 trxA 886558 trxA Mycobacterium tuberculosis H37Rv Probable thioredoxin TrxA NP_215986.1 1658980 D 83332 CDS YP_177815.1 57116870 886554 1659370..1659741 1 NC_000962.3 Rv1471, (MTV007.18), len: 123 aa. Probable trxB1,thioredoxin, similar to many bacterial thioredoxins e.g. P33636|THI2_ECOLI from Escherichia coli (139 aa), FASTA scores: opt: 290, E(): 1.8e-13, (44.3% identity in 97 aa overlap); etc. Highly similar to Rv1470|TrxA probable thioredoxin from Mycobacterium tuberculosis (124 aa), FASTA scores: opt: 402, E(): 1.2e-32, (54.4% identity in 114 aa overlap). Contains PS00194 Thioredoxin family active site. Belongs to the thioredoxin family. Note that previously known as trxB.; Probable thioredoxin TrxB1 1659741 trxB1 886554 trxB1 Mycobacterium tuberculosis H37Rv Probable thioredoxin TrxB1 YP_177815.1 1659370 D 83332 CDS NP_215988.1 15608610 886547 1659763..1660620 1 NC_000962.3 Rv1472, (MTV007.19), len: 285 aa. Possible echA12,enoyl-CoA hydratase, highly similar to P53526|ECHH_MYCLE|NP_301896.1|NC_002677 possible enoyl-CoA hydratase/isomerase from Mycobacterium leprae (294 aa),FASTA scores: opt: 1265, E(): 0, (72.0% identity in 271 aa overlap). Also similar to others e.g. CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli strain K12 (262 aa); CAC44593.1|AL596162 putative enoyl-CoA hydratase from Streptomyces coelicolor (275 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. ECHA16|Rv2831|MTCY16B7.11c (249 aa), FASTA scores: opt: 232, E(): 1.3e-15, (33.8% identity in 204 aa overlap); etc.; Possible enoyl-CoA hydratase EchA12 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 1660620 echA12 886547 echA12 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase EchA12 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_215988.1 1659763 D 83332 CDS NP_215989.1 15608611 886549 1660656..1662284 1 NC_000962.3 Rv1473, (MTV007.20), len: 542 aa. Possible macrolide-transport ATP-binding protein ABC transporter (see citation below), possibly in EF-3 subfamily. Similar to many ABC-transporters e.g. D90909_48|YHES_HAEIN from Synechocystis sp. strain PCC6803 (574 aa), FASTA scores: opt: 870, E(): 0, (33.3% identity in 525 aa overlap); P44808|YHES_HAEIN from Haemophilus influenzae (638 aa),FASTA scores: opt: 706, E(): 0, (33.7% identity in 517 aa overlap); etc. Contains two PS00017 ATP/GTP-binding site motif A (P-loop), and two PS00211 ABC transporter family signatures. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable macrolide-transport ATP-binding protein ABC transporter 1662284 886549 Rv1473 Mycobacterium tuberculosis H37Rv Probable macrolide-transport ATP-binding protein ABC transporter NP_215989.1 1660656 D 83332 CDS YP_177644.1 57116871 3205054 1662381..1662572 1 NC_000962.3 Rv1473A, len: 63 aa. Possible transcriptional regulator, CDS predicted by GC plot. Similar to SCI8.24c|AL132644_24 putative transcriptional regulator from Streptomyces coelicolor (73 aa), FASTA scores: opt: 210, E(): 1.5e-08, (56.15% identity in 57 aa overlap).; Possible transcriptional regulatory protein 1662572 3205054 Rv1473A Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein YP_177644.1 1662381 D 83332 CDS NP_215990.1 15608612 886543 complement(1662641..1663204) 1 NC_000962.3 Rv1474c, (MTV007.21c), len: 187 aa. Probable transcription regulator, equivalent to AF0021|AF002133_1 transcriptional regulator from Mycobacterium avium strain GIR10 (82 aa), FASTA scores: opt: 490, E(): 6.7e-26, (92.5% identity in 80 aa overlap). Also similar to Q59431|UIDR_ECOLI UID operon repressor (GUS operon) from Escherichia coli (196 aa), FASTA scores: opt: 192, E(): 5.8e-06, (28.5% identity in 172 aa overlap). Belongs to the TetR/AcrR family of transcriptional regulators. Helix turn helix motif predicted at aa 33-54 (+3.40 SD).; Probable transcriptional regulatory protein 1663204 886543 Rv1474c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_215990.1 1662641 R 83332 CDS NP_215991.1 15608613 886545 complement(1663215..1666046) 1 NC_000962.3 Rv1475c, (MTV007.22c), len: 943 aa. Probable acn,iron-regulated aconitate hydratase, similar to many e.g. P70920|ACON_BRAJA aconitate hydratase from Bradyrhizobium japonicum (906 aa), FASTA scores: opt:1912, E(): 0, (54.8% identity in 958 aa overlap); closest to AF0021|AF002133_2 Mycobacterium avium strain GIR10 (961 aa), FASTA scores: opt: 5072, E(): 0, (82.8% identity in 943 aa overlap). Note aconitase has an active (4FE-4S) and an inactive (3FE-4S) forms. The active (4FE-4S) cluster is part of the catalytic site that interconverts citrate, cis-aconitase, and isocitrate.; Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase) 1666046 acn 886545 acn Mycobacterium tuberculosis H37Rv Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase) NP_215991.1 1663215 R 83332 CDS NP_215992.1 15608614 886539 1666204..1666764 1 NC_000962.3 Rv1476, (MTV007.23), len: 186 aa. Possibly membrane protein, TMhelix 138-60. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Possible membrane protein 1666764 886539 Rv1476 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_215992.1 1666204 D 83332 CDS NP_215993.1 15608615 886541 1666990..1668408 1 NC_000962.3 Rv1477, (MTV007.24), len: 472 aa. RipA,peptidoglycan hydrolase (see Hett et al., 2007). Secreted,cell-associated protein. The last 277 residues are nearly identical to those of AF0060|AF006054_1 hypothetical invasion protein INV1 from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 1833, E(): 0, (98.2% identity in 277 aa overlap); also very similar to AF0021|AF002133_4 invasin 1 protein from Mycobacterium avium (273 aa), FASTA scores: opt: 1452, E(): 0, (78.1% identity in 279 aa overlap). Similar to Rv1566c|MTCY336.37|Z95586 Mycobacterium tuberculosis cosmid (230 aa), FASTA scores: opt: 528, E(): 4.4e-20, (52.0% identity in 150 aa overlap); and weakly similar to p60 proteins of Listeria spp throughout its length e.g. M80351|LISIAPB_1 Listeria monocytogenes iap-related protein (478 aa), FASTA scores: opt: 251, E(): 8e-06, (24.4% identity in 487 aa overlap). C-terminal domain highly similar to next orf Rv1478|MTV007.25. Interacts with RpfB and RpfE (see Hett et al., 2007). Predicted to be an outer membrane protein (See Song et al., 2008).; Peptidoglycan hydrolase 1668408 ripA 886541 ripA Mycobacterium tuberculosis H37Rv Peptidoglycan hydrolase NP_215993.1 1666990 D 83332 CDS NP_215994.1 15608616 886535 1668419..1669144 1 NC_000962.3 Rv1478, (MTV007.25), len: 241 aa. Possible invasion protein. Possibly exported protein, nearly identical to AF0060|AF006054_2 hypothetical invasion protein INV2 of Mycobacterium tuberculosis (240 aa), FASTA scores: opt: 1509, E(): 0, (95.0% identity in 241 aa overlap); very similar to AF0021|AF002133_5 hypothetical invasion protein INV2 from Mycobacterium avium (244 aa), FASTA scores: opt: 1269, E():0, (78.0% identity in 246 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY336.37 and weakly similar to C-terminal segment of p60 proteins of Listeria spp.e.g. Q01836|P60_LISIN protein P60 precursor (481 aa), FASTA scores: opt: 241, E():4e-07, (37.7% identity in 122 aa overlap). Highly similar to C-terminal domain of preceeding ORF Rv1477|MTV007.24 (472 aa), FASTA scores: opt: 864, E(): 0, (60.1% identity in 213 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible invasion protein 1669144 886535 Rv1478 Mycobacterium tuberculosis H37Rv Possible invasion protein NP_215994.1 1668419 D 83332 CDS YP_177816.1 57116872 886537 1669283..1670416 1 NC_000962.3 Rv1479, (MTV007.26), len: 377 aa. Probable moxR1,transcriptional regulatory protein, similar to X96434|BBGIDBMOX_2 moxR regulator from Borrelia burgdorferi (329 aa), FASTA scores: opt: 850, E():0, (43.5% identity in 317 aa overlap); and P. denitrificans. Highly similar to MoxR homologs of Mycobacterium tuberculosis and Mycobacterium avium (but these both differ at C-terminus) e.g. Rv3692, Rv3164c, and AF0021|AF002133_6 Mycobacterium avium strain GIR10 (309 aa), FASTA scores: opt: 1181, E(): 0, (83.7% identity in 227 aa overlap). Also similar to O33173|AF006054 MoxR fragment from Mycobacterium tuberculosis (211 aa), FASTA scores: opt: 1305, E(): 0,(94.3% identity in 212 aa overlap). Note that previously known as moxR.; Probable transcriptional regulatory protein MoxR1 1670416 moxR1 886537 moxR1 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein MoxR1 YP_177816.1 1669283 D 83332 CDS NP_215996.1 15608618 886531 1670413..1671366 1 NC_000962.3 Rv1480, (MTV007.27,MTCY227.01), len: 317 aa. Conserved protein, last 110 aa residues correspond to first 110 aa of YS01_MYCAV|O07394 hypothetical 18.7 kDa Mycobacterium avium protein MAV169 (169 aa), FASTA scores: opt: 642, E(): 0, (84.2% identity in 114 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv3163c and Rv3693.; hypothetical protein 1671366 886531 Rv1480 Mycobacterium tuberculosis H37Rv hypothetical protein NP_215996.1 1670413 D 83332 CDS NP_215997.1 15608619 886533 1671377..1672384 1 NC_000962.3 Rv1481, (MTCY277.02), len: 335 aa. Probable membrane protein, highly similar to YS02_MYCAV|O07395 hypothetical 36.1 kDa protein mav335 from Mycobacterium avium (335 aa),FASTA scores: opt: 1904, E(): 0, (89.0% identity in 337 aa overlap). Similar to AF116251|AF116251_1 BatA protein from Bacteroides fragilis (327 aa), FASTA scores: opt: 317, E(): 2e-12, (26.5% identity in 340 aa overlap).; Probable membrane protein 1672384 886533 Rv1481 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_215997.1 1671377 D 83332 CDS NP_215998.2 57116873 886526 complement(1672457..1673299) 1 NC_000962.3 Rv1482c, (MTCY277.03c), len: 280 aa. Conserved hypothetical protein, highly similar to O07396|AF002133 Mycobacterium avium protein MAV346 (346 aa), FASTA scores: E(): 0, (65.2% identity in 342 aa overlap); slight similarity to GRPE_ECOLI|P09372 heat shock protein from E. coli (197 aa), FASTA scores: opt: 139, E(): 0.012, (28.3% identity in 159 aa overlap). Similar to Mycobacterium tuberculosis hypothetical proteins Rv3517, Rv3555c,Rv3714c, Rv1073, etc. Start changed since first submission (-59 aa).; hypothetical protein 1673299 886526 Rv1482c Mycobacterium tuberculosis H37Rv hypothetical protein NP_215998.2 1672457 R 83332 CDS NP_215999.1 15608621 886551 1673440..1674183 1 NC_000962.3 Rv1483, (MTCY277.04), len: 247 aa. FabG1 (alternate gene name: mabA), 3-oxoacyl-[acyl-carrier protein] reductase (see citations below), equivalent to O07399|FABG_MYCAV 3-oxoacyl-[acyl-carrier protein] reductase from Mycobacterium avium (255 aa); P71534|FABG_MYCSM 3-oxoacyl-[acyl-carrier protein] reductase from Mycobacterium smegmatis (255 aa); and NP_302228.1|NC_002677 3-oxoacyl-[ACP] reductase (aka MabA) from Mycobacterium leprae (253 aa). Also highly similar to many e.g. T36779 probable 3-oxacyl-(acyl-carrier-protein) reductase from Streptomyces coelicolor (234 aa); FABG_ECOLI|P25716|NP_415611.1|NC_000913 3-oxoacyl-[acyl-carrier-protein] reductase from Escherichia coli strain K12 (244 aa), FASTA scores: opt: 664, E(): 6.8e-35, (44.4% identity in 241 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; 3-oxoacyl-[acyl-carrier protein] reductase FabG1 (3-ketoacyl-acyl carrier protein reductase) (mycolic acid biosynthesis a protein) 1674183 fabG1 886551 fabG1 Mycobacterium tuberculosis H37Rv 3-oxoacyl-[acyl-carrier protein] reductase FabG1 (3-ketoacyl-acyl carrier protein reductase) (mycolic acid biosynthesis a protein) NP_215999.1 1673440 D 83332 CDS NP_216000.1 15608622 886523 1674202..1675011 1 NC_000962.3 Rv1484, (MTCY277.05), len: 269 aa. InhA,NADH-dependent enoyl-[acyl-carrier-protein] reductase (see citations below). Identical to INHA_MYCTU|P46533 enoyl-[acyl-carrier-protein] reductase from Mycobacterium tuberculosis and G1155270 Mycobacterium bovis enoyl acp reductase. Some similarity to the short-chain dehydrogenases/reductases (SDR) family.; NADH-dependent enoyl-[acyl-carrier-protein] reductase InhA (NADH-dependent enoyl-ACP reductase) 1675011 inhA 886523 inhA Mycobacterium tuberculosis H37Rv NADH-dependent enoyl-[acyl-carrier-protein] reductase InhA (NADH-dependent enoyl-ACP reductase) NP_216000.1 1674202 D 83332 CDS NP_216001.1 15608623 886525 1675017..1676051 1 NC_000962.3 Rv1485, (MTCY277.06), len: 344 aa. HemZ,ferrochelatase (see citation below), similar to many e.g. HEMZ_BACSU|P32396 ferrochelatase from Bacillus subtilus (310 aa), FASTA scores: opt:490, E(): 2e-24, (30.2% identity in 295 aa overlap); etc. Belongs to the ferrochelatase family.; Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase) 1676051 hemZ 886525 hemZ Mycobacterium tuberculosis H37Rv Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase) NP_216001.1 1675017 D 83332 CDS NP_216002.2 57116874 886519 complement(1676017..1676883) 1 NC_000962.3 Rv1486c, (MTCY277.07c), len: 288 aa. Conserved hypothetical protein, highly similar to YS07_MYCAV|O07402 hypothetical 33.5 kDa protein mav321 from Mycobacterium avium (320 aa), FASTA scores: opt: 1217, E(): 0, (71.1% identity in 315 aa overlap). Weak similarity to AL079332|SCI5.07 hypothetical protein from Streptomyces coelicolor (259 aa), FASTA scores: opt: 131, E(): 0.29,(32.3% identity in 279 aa overlap). Start changed since original submission.; hypothetical protein 1676883 886519 Rv1486c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216002.2 1676017 R 83332 CDS NP_216003.1 15608625 886521 1676941..1677375 1 NC_000962.3 Rv1487, (MTCY277.08), len: 144 aa. Conserved membrane protein. Highly similar to O07404|AF002133 MAV145 from Mycobacterium avium (145 aa), FASTA scores: opt: 667,E(): 0, (72.5% identity in 142 aa overlap). Also similar to AL079332|SCI5.05 hypothetical protein from Streptomyces coelicolor (143 aa), FASTA scores: opt: 344, E(): 1.3e-15,(44.8% identity in 134 aa overlap).; hypothetical protein 1677375 886521 Rv1487 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216003.1 1676941 D 83332 CDS NP_216004.1 15608626 886515 1677397..1678542 1 NC_000962.3 Rv1488, (MTCY277.09), len: 381 aa. Possible exported conserved protein; contains possible N-terminal signal sequence. Similar to YBBK_ECOLI|P77367 hypothetical protein ybbK from Escherichia coli (305 aa), FASTA scores: opt: 716, E(): 0, (37.1% identity in 307 aa overlap). Similar to stomatin-like proteins e.g. AF065260|AF065260_1 Clostridium difficile (320 aa), FASTA scores: opt: 767, E(): 0, (42.3% identity in 307 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible exported conserved protein 1678542 886515 Rv1488 Mycobacterium tuberculosis H37Rv Possible exported conserved protein NP_216004.1 1677397 D 83332 CDS YP_177645.1 57116875 3205064 1678552..1678908 1 NC_000962.3 Rv1489, len: 118 aa. Conserved protein, similar to hypothetical proteins from Mycobacterium avium subsp. paratuberculosis and Streptomyces coelicolor e.g. AJ250017_1 insertion sequence IS900, Locus 3, putative invasion protein from M. paratuberculosis (138 aa), FASTA scores: opt: 120, E(): 0.26, (34.375% identity in 96 aa overlap); SCD6.11c|AL353815_11 possible integral membrane protein from Streptomyces coelicolor (136 aa), FASTA scores: opt: 106, E(): 2.2, (35.9% identity in 103 aa overlap). ORF predicted by GC plot. Replaces previous Rv1489c on other strand.; hypothetical protein 1678908 3205064 Rv1489 Mycobacterium tuberculosis H37Rv hypothetical protein YP_177645.1 1678552 D 83332 CDS YP_177646.1 57116876 3205065 1678942..1679172 1 NC_000962.3 Rv1489A, len: 76 aa. Conserved protein, similar to part of alpha subunit of many methylmalonyl-CoA mutases (~750 aa). Size difference suggests possible gene fragment although Mycobacterium tuberculosis has intact methylmalonyl-CoA mutase gene. P71774|MUTB_MYCTU probable methylmalonyl-CoA mutase from Mycobacterium tuberculosis (750 aa), FASTA scores: opt: 258, E(): 3.2e-10, (73.35% identity in 60 aa overlap). ORF predicted by GC plot.; hypothetical protein 1679172 3205065 Rv1489A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177646.1 1678942 D 83332 CDS NP_216006.1 15608628 886511 1679322..1680629 1 NC_000962.3 Rv1490, (MTCY277.12), len: 435 aa. Probable membrane protein.; Probable membrane protein 1680629 886511 Rv1490 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216006.1 1679322 D 83332 CDS NP_216007.1 15608629 886513 complement(1681208..1681966) 1 NC_000962.3 Rv1491c, (MTCY277.13c), len: 252 aa. Conserved membrane protein. Similar to hypothetical proteins from many organisms e.g. YDJZ_ECOLI|P76221 Escherichia coli (235 aa), FASTA scores: opt: 223, E():6.7 e-07, (31.7% identity in 145 aa overlap); AL133252|SCE46.15 Streptomyces coelicolor (249 aa), FASTA scores: opt: 378, E(): 1.5e-17,(39.1% identity in 169 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical protein Rv0625c.; hypothetical protein 1681966 886513 Rv1491c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216007.1 1681208 R 83332 CDS NP_216008.1 15608630 886507 1682157..1684004 1 NC_000962.3 Rv1492, (MTCY277.14), len: 615 aa. Probable mutA,Methylmalonyl-CoA mutase small-subunit, strong similarity to e.g. MUTA_STRCM|Q05064 methylmalonyl-CoA mutase beta-subunit from Streptomyces cinnamonensis (616 aa),FASTA scores: opt: 1512, E(): 0, (45.9% identity in 628 aa overlap). Contains PS00213 Lipocalin signature, PS00544 Methylmalonyl-CoA mutase signature. Belongs to the methylmalonyl-CoA mutase family.; Probable methylmalonyl-CoA mutase small subunit MutA (MCM) 1684004 mutA 886507 mutA Mycobacterium tuberculosis H37Rv Probable methylmalonyl-CoA mutase small subunit MutA (MCM) NP_216008.1 1682157 D 83332 CDS NP_216009.1 15608631 886509 1684005..1686257 1 NC_000962.3 Rv1493, (MTCY277.15), len: 750 aa. Probable mutB,Methylmalonyl-CoA mutase large-subunit, strong similarity to e.g. MUTB_STRCM|Q05065 methylmalonyl-CoA mutase alpha-subunit from Streptomyces cinnamonensis (733 aa),FASTA scores: opt: 3562, E(): 0, (75.8% identity in 730 aa overlap). Contains PS00544 Methylmalonyl-CoA mutase signature. Belongs to the methylmalonyl-CoA mutase family.; Probable methylmalonyl-CoA mutase large subunit MutB (MCM) 1686257 mutB 886509 mutB Mycobacterium tuberculosis H37Rv Probable methylmalonyl-CoA mutase large subunit MutB (MCM) NP_216009.1 1684005 D 83332 CDS NP_216010.1 15608632 886502 1686271..1686573 1 NC_000962.3 Rv1494, (MTCY277.16), len: 100 aa. Possible mazE4,antitoxin, part of toxin-antitoxin (TA) operon with Rv1495 (See Pandey and Gerdes, 2005; Zhu et al., 2006).; Possible antitoxin MazE4 1686573 mazE4 886502 mazE4 Mycobacterium tuberculosis H37Rv Possible antitoxin MazE4 NP_216010.1 1686271 D 83332 CDS NP_216011.1 15608633 886504 1686570..1686887 1 NC_000962.3 Rv1495, (MTCY277.17), len: 105 aa. Possible mazF4,toxin, part of toxin-antitoxin (TA) operon with Rv1494 (See Pandey and Gerdes, 2005; Zhu et al., 2006), some similarity to Rv1942c|MTCY09F9.22 hypothetical protein from Mycobacterium tuberculosis (109 aa) (0.7% identity in 101 aa overlap) and Rv0659c, Rv1102c.; Possible toxin MazF4 1686887 mazF4 886504 mazF4 Mycobacterium tuberculosis H37Rv Possible toxin MazF4 NP_216011.1 1686570 D 83332 CDS NP_216012.1 15608634 886496 1686884..1687888 1 NC_000962.3 Rv1496, (MTCY277.18), len: 334 aa. Possible transport system kinase. Equivalent to NP_302220.1|NC_002677 putative kinase from Mycobacterium leprae (327 aa). Highly similar to several transport system kinases and NTPase transporters e.g. P27254|ARGK_ECOLI|B2918 LAO/AO transport system kinase from Escherichia coli K12 (331 aa) (see citation below); NP_311815.1|NC_002695 ATPase component of two convergent arginine transporter from Escherichia coli O157:H7 (331 aa); etc. Also similar to YPLE_CAUCR|P37895 hypothetical 34.6 kDa protein in Caulobacter crescentus (326 aa), FASTA scores, opt: 1125, E(): 0, (55.7% identity in 316 aa overlap).; Possible transport system kinase 1687888 886496 Rv1496 Mycobacterium tuberculosis H37Rv Possible transport system kinase NP_216012.1 1686884 D 83332 CDS NP_216013.1 15608635 886575 1687941..1689230 1 NC_000962.3 Rv1497, (MTCY277.19), len: 429 aa. Probable LipL,esterase, very similar to Mycobacterium tuberculosis hypothetical esterases and penicillin binding proteins e.g. Rv1923, Rv2463, Rv3775, etc. Also similar to G151214|M68491 esterase estA from Pseudomonas sp (389 aa), FASTA scores: opt: 604, E(): 1e-31, (34.4% identity in 389 aa overlap).; Probable esterase LipL 1689230 lipL 886575 lipL Mycobacterium tuberculosis H37Rv Probable esterase LipL NP_216013.1 1687941 D 83332 CDS NP_216014.1 15608636 886503 complement(1689303..1689920) 1 NC_000962.3 Rv1498c, (MTCY277.20c), len: 205 aa. Probable methyltransferase. Similar to G2792343|AF040571 methyltransferase from amycolatopsis mediterranei (272 aa),FASTA scores: E(): 5.1e-11, (32.3% identity in 124 aa overlap). Contains PS00017 ATP/GTP-binding site motif A.; Probable methyltransferase 1689920 886503 Rv1498c Mycobacterium tuberculosis H37Rv Probable methyltransferase NP_216014.1 1689303 R 83332 CDS YP_177647.1 57116877 3205040 complement(1690134..1690346) 1 NC_000962.3 Rv1498A, len: 70 aa. Conserved protein, highly similar to other hypothetical proteins e.g. from Streptomyces coelicolor, Sinorhizobium meliloti and Pseudomonas aeruginosa.; hypothetical protein 1690346 3205040 Rv1498A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177647.1 1690134 R 83332 CDS NP_216015.2 57116878 886494 1690407..1690805 1 NC_000962.3 Rv1499, (MTCY277.21), len: 132 aa. Hypothetical unknown protein; was initially longer but has been shortened (-24 aa) owing to overlap with Rv1498A. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 1690805 886494 Rv1499 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216015.2 1690407 D 83332 CDS NP_216016.1 15608638 886492 1690850..1691878 1 NC_000962.3 Rv1500, (MTCY277.22), len: 342 aa. Probable glycosyltransferase, hydrophobic domain near C-terminus. Some similarity to putative glycosyl-transferases from Bacillus subtilis e.g. O34319|YKCC_BACSU (323 aa), opt: 490, E(): 6.1e-25, (28.85% identity in 312 aa overlap) and to N-acetyl glucosamine transferases. Also similar to G1001347 hypothetical 36.7 kDa protein (318 aa), FASTA scores: opt: 523, E(): 7.2e-26, (30.6% identity in 307 aa overlap).; Probable glycosyltransferase 1691878 886492 Rv1500 Mycobacterium tuberculosis H37Rv Probable glycosyltransferase NP_216016.1 1690850 D 83332 CDS NP_216017.1 15608639 886499 1691890..1692711 1 NC_000962.3 Rv1501, (MTCY277.23), len: 273 aa. Conserved hypothetical protein, some similarity to O06374|Rv3633|MTCY15C10.19C hypothetical protein from Mycobacterium tuberculosis, FASTA scores: E(): 3.9e-10,(27.5% identity in 280 aa overlap).; hypothetical protein 1692711 886499 Rv1501 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216017.1 1691890 D 83332 CDS NP_216018.1 15608640 886486 1692924..1693823 1 NC_000962.3 Rv1502, (MTCY277.24), len: 299 aa. Hypothetical unknown protein.; Hypothetical protein 1693823 886486 Rv1502 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216018.1 1692924 D 83332 CDS NP_216019.1 15608641 886488 complement(1693996..>1694544) 1 NC_000962.3 Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 565,E(): 0, (49.4% identity in 170 aa overlap); Rv1503c and Rv1504c are both similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene.; hypothetical protein >1694544 886488 Rv1503c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216019.1 1693996 R 83332 CDS NP_216020.1 15608642 886481 complement(1694545..1695144) 1 NC_000962.3 Rv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 863,E(): 0, (68.0% identity in 194 aa overlap); Rv1503c and Rv1504c are similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene.; hypothetical protein 1695144 886481 Rv1504c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216020.1 1694545 R 83332 CDS NP_216021.1 15608643 886483 complement(1695281..1695946) 1 NC_000962.3 Rv1505c, (MTCY277.27c), len: 221 aa. Conserved hypothetical protein, some similarity to hypothetical proteins and glycosylases e.g. P71063|O08181 hypothetical 22.5 kDa protein YVFD from Bacillus subtilis (216 aa),FASTA scores: E(): 2.4e-08, (25.5% identity in 196 aa overlap).; hypothetical protein 1695946 886483 Rv1505c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216021.1 1695281 R 83332 CDS NP_216022.1 15608644 886479 complement(1695943..1696443) 1 NC_000962.3 Rv1506c, (MTCY277.28c), len: 166 aa. Hypothetical unknown protein.; Hypothetical protein 1696443 886479 Rv1506c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216022.1 1695943 R 83332 CDS NP_216023.1 15608645 886477 complement(1696727..1697422) 1 NC_000962.3 Rv1507c, (MTCY277.29c), len: 231 aa. Conserved protein. Similar to AJ007747|BBR007747_6 Hypothetical protein BbLPS1.06 from Bordetella bronchiseptica cosmid (239 aa), FASTA scores: opt: 362, E(): 1.3e-17, (30.8% identity in 221 aa overlap).; hypothetical protein 1697422 886477 Rv1507c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216023.1 1696727 R 83332 CDS YP_177648.1 57116879 3205095 1697356..1697859 1 NC_000962.3 Rv1507A, len: 167 aa. Hypothetical unknow protein. Shows weak similarity with C-terminus of Q9XHQ7|CDA9 cytidine deaminase 9 from Arabidopsis thaliana (Mouse-ear cress) (298 aa), FASTA scores: opt: 104, E(): 4.2, (33.6% identity in 133 aa overlap), blastp scores: Score: 77,Identities: 39/133 (29%), Positives: 62/133 (46%).; Hypothetical protein 1697859 3205095 Rv1507A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177648.1 1697356 D 83332 CDS NP_216024.1 15608646 886078 complement(1698095..1699894) 1 NC_000962.3 Rv1508c, (MTCY277.30c), len: 599 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable membrane protein.; Probable membrane protein 1699894 886078 Rv1508c Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216024.1 1698095 R 83332 CDS YP_177649.1 57116880 3205096 1699866..1700228 1 NC_000962.3 Rv1508A, len: 120 aa. Conserved hypothetical protein, highly similar to central part of glycosyl transferases from various mycobacteria and eubacteria e.g. P71790|MTCY277.33|Rv1511 Hypothetical protein from M. tuberculosis (340 aa), FASTA scores: opt: 210, E(): 2.5 e-09, (42.9% identity in 105 aa overlap).; hypothetical protein 1700228 3205096 Rv1508A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177649.1 1699866 D 83332 CDS NP_216025.1 15608647 886475 1700212..1701093 1 NC_000962.3 Rv1509, (MTCY277.31), len: 293 aa. Hypothetical unknown protein.; Hypothetical protein 1701093 886475 Rv1509 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216025.1 1700212 D 83332 CDS NP_216026.1 15608648 886466 1701295..1702593 1 NC_000962.3 Rv1510, (MTCY277.32), len: 432 aa. Probable membrane protein. Highly similar to Rv3630|MTCY15C10.22 (431 aa),FASTA scores: E(): 0, (70.8% identity in 424 aa overlap).; Conserved probable membrane protein 1702593 886466 Rv1510 Mycobacterium tuberculosis H37Rv Conserved probable membrane protein NP_216026.1 1701295 D 83332 CDS NP_216027.1 15608649 886529 1703074..1704096 1 NC_000962.3 Rv1511, (MTCY277.33), len: 340 aa. Probable gmdA,GDP-D-mannose dehydratase, equivalent to AF125999|AF125999_13 Mycobacterium avium enzyme (343 aa),FASTA scores: opt: 2085, E(): 0, (89.1% identity in 338 aa overlap); similar to G755218 pseudomonas aeruginosa GDP-D-mannose dehydratase (GCA) (323 aa), FASTA scores: opt: 1073, E(): 0, (51.9% identity in 320 aa overlap); and to S74433 GDP-D-mannose dehydratase rfbD - Syn (362 aa),FASTA scores: opt: 1405, E(): 0, (63.9% identity in 327 aa overlap).; GDP-D-mannose dehydratase GmdA (GDP-mannose 4,6 dehydratase) (GMD) 1704096 gmdA 886529 gmdA Mycobacterium tuberculosis H37Rv GDP-D-mannose dehydratase GmdA (GDP-mannose 4,6 dehydratase) (GMD) NP_216027.1 1703074 D 83332 CDS NP_216028.1 15608650 886461 1704093..1705061 1 NC_000962.3 Rv1512, (MTCY277.34), len: 322 aa. Probable epiA,nucleotide sugar epimerase, equivalent to AJ223832|MAS223832_4 from Mycobacterium avium silvaticum (339 aa), FASTA scores: opt: 1821, E(): 0, (84.6% identity in 318 aa overlap); and similar to WCAG_ECOLI|P32055 colanic acid biosynthesis protein wcaG (321 aa), FASTA scores: opt: 835, E(): 0, (53.5% identity in 316 aa overlap).; Probable nucleotide-sugar epimerase EpiA 1705061 epiA 886461 epiA Mycobacterium tuberculosis H37Rv Probable nucleotide-sugar epimerase EpiA NP_216028.1 1704093 D 83332 CDS NP_216029.1 15608651 886464 1705058..1705789 1 NC_000962.3 Rv1513, (MTCY277.35), len: 243 aa. Conserved protein, similar to hypothetical proteins from several organisms e.g. AJ223833|MAP223833_3 from Mycobacterium avium paratuberculosis (240 aa), FASTA scores: opt: 1053 E(): 0, (66.3% identity in 243 aa overlap); P74191|SLL1173 from Synechocystis (244 aa), FASTA scores: opt: 276, E(): 1.1e-07, (32.2 % identity in 202 aa overlap). Also highly similar to P95136|Q50460|MTCY349.33c|Rv2956 from Mycobacterium tuberculosis (243 aa), (70.0% identity in 237 aa overlap).; hypothetical protein 1705789 886464 Rv1513 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216029.1 1705058 D 83332 CDS NP_216030.1 15608652 886457 complement(1705807..1706595) 1 NC_000962.3 Rv1514c, (MTCY277.36c), len: 262 aa. Conserved hypothetical protein. Similar to other hypothetical proteins, and to WCAE_ECOLI|P71239 putative colanic acid biosynthesis glycosyl transferase (248 aa), FASTA scores: opt: 231, E(): 4.1e-08, (33.3% identity in 210 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical glycosyltransferase, Rv2957.; hypothetical protein 1706595 886457 Rv1514c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216030.1 1705807 R 83332 CDS NP_216031.1 15608653 886459 complement(1706630..1707526) 1 NC_000962.3 Rv1515c, (MTCY277.37c), len: 298 aa. Conserved hypothetical protein, similar to P71805|MTCY02B12.11C|Rv1377c Hypothetical protein from Mycobacterium tuberculosis, FASTA scores: E(): 1.3e-05,(25.4% identity in 134 aa overlap).; hypothetical protein 1707526 886459 Rv1515c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216031.1 1706630 R 83332 CDS NP_216032.2 57116881 886455 complement(1707529..1708539) 1 NC_000962.3 Rv1516c, (MTCY277.38c), len: 336 aa. Probable sugar transferase, similar to AB010970|AB010970_6 glycosyltransferase from Streptococcus mutans (465 aa),FASTA scores: opt: 388, E(): 4.1e-18, (32.7% identity in 214 aa overlap), slight similarity to SPSA_BACSU|P39621 spore coat polysaccharide biosynthesis (256 aa), fasta scores: opt: 185, E(): 6.5e-05, (26.2% identity in 187 aa overlap), strong similarity to Rv1520|MTCY19G5.08c probable sugar transferase from Mycobacterium tuberculosis (63.5% identity in 318 aa overlap).; Probable sugar transferase 1708539 886455 Rv1516c Mycobacterium tuberculosis H37Rv Probable sugar transferase NP_216032.2 1707529 R 83332 CDS NP_216033.1 15608655 886467 1708871..1709635 1 NC_000962.3 Rv1517, (MTCY277.39), len: 254 aa. Conserved hypothetical transmembrane protein, similar to G466802|LEPB1170_F2_64 from Mycobacterium leprae (230 aa),FASTA scores: opt: 282, E(): 2.2e-11, (34.1% identity in 255 aa overlap). Also similar to Mycobacterium tuberculosis Rv3821|MTCY409.09c (237 aa) (36.3% identity in 256 aa overlap); and Rv3481c.; hypothetical protein 1709635 886467 Rv1517 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216033.1 1708871 D 83332 CDS NP_216034.1 15608656 886451 1709644..1710603 1 NC_000962.3 Rv1518, (MTCY277.40, MTCY19G5.11c), len: 319 aa. Conserved hypothetical protein, possibly glycosyl transferase involved in exopolysaccharide synthesis,similar to several hypothetical proteins and glycosyl transferases from diverse organisms e.g. P73996|D90911 from synecho cystis sp. (309 aa), Fasta scores: opt: 300, E(): 1.8e-13, (29.5% identity in 241 aa overlap).; hypothetical protein 1710603 886451 Rv1518 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216034.1 1709644 D 83332 CDS NP_216035.1 15608657 886453 1710733..1711002 1 NC_000962.3 Rv1519, (MTCY19G5.09c), len: 89 aa. Conserved hypothetical protein, high similarity to C-terminus of Q50723|MTCY78.26|Rv3402c (412 aa) (58.1% identity in 74 aa overlap).; hypothetical protein 1711002 886453 Rv1519 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216035.1 1710733 D 83332 CDS NP_216036.3 448824758 886447 1711028..1712068 1 NC_000962.3 Rv1520, (MTCY19G5.08c), len: 346 aa. Probable sugar transferase, similar to several e.g. AB010970|AB010970_6 Streptococcus mutans glycosyltransferase (465 aa), FASTA scores: opt: 381, E(): 1.2e-18, (31.7% identity in 240 aa overlap); O34234|Y07786 sugar transferase from Vibrio cholerae (337 aa), FASTA scores: opt: 214, E(): 8.4e-05,(25.9% identity in 212 aa overlap). Also strongly similar to Mycobacterium tuberculosis probable sugar transferase Rv1516c. Alternative nucleotide at position 1711627 (C->T; Y200Y) has been observed.; Probable sugar transferase 1712068 886447 Rv1520 Mycobacterium tuberculosis H37Rv Probable sugar transferase NP_216036.3 1711028 D 83332 CDS NP_216037.1 15608659 886448 1712302..1714053 1 NC_000962.3 Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25,fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc.; Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 1714053 fadD25 886448 fadD25 Mycobacterium tuberculosis H37Rv Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_216037.1 1712302 D 83332 CDS NP_216038.1 15608660 886445 complement(1714172..1717612) 1 NC_000962.3 Rv1522c, (MTCY19G5.06), len: 1146 aa. Probable mmpL12, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily. Strong similarity to many Mycobacterial membrane proteins e.g. Q49619|G466786 putative transport protein B1170_C1_181 from Mycobacterium leprae (1008 aa), FASTA scores: opt: 2418,E(): 0, (51.0% identity in 1006 aa overlap); etc. Also highly similar to MmpL8|MTCY48.08c|Rv3823c probable conserved transmembrane transport protein from Mycobacterium tuberculosis, FASTA score: (34.3% identity in 376 aa overlap); and some similarity to MmpL10|MTCY20G9|Rv1183 probable conserved transmembrane transport protein, FASTA score: (27.2% identity in 1011 aa overlap). Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL12 1717612 mmpL12 886445 mmpL12 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL12 NP_216038.1 1714172 R 83332 CDS NP_216039.1 15608661 886489 1717653..1718696 1 NC_000962.3 Rv1523, (MTCY19G5.05c), len: 347 aa (start uncertain). Probable methyltransferase, similar to G560513|U0002O Mycobacterium leprae (270 aa), FASTA scores: opt: 965, E(): 0, (60.3% identity in 247 aa overlap). Also similar to many e.g. Q54303|X86780 methyltransferase RAPM from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 323, E(): 1e-15, (41.2% identity in 136 aa overlap). And similar to M. tuberculosis hypothetical proteins Rv2952, Rv1405c, Rv1403c, Rv0839.; Probable methyltransferase 1718696 886489 Rv1523 Mycobacterium tuberculosis H37Rv Probable methyltransferase NP_216039.1 1717653 D 83332 CDS NP_216040.1 15608662 885814 1718726..1719970 1 NC_000962.3 Rv1524, (MTCY19G5.04c), len: 414 aa. Probable glycosyltransferase, similar to many e.g. P96559|U84349 glycosyltransferase GTFB from Amycolatopsis orientalis (407 aa), FASTA scores: opt: 363, E(): 6.2e-23, (28.8% identity in 430 aa overlap); also high similarity to Rv1526c|MTCY19G5.02 Mycobacterium tuberculosis hypothetical protein (58.7% identity in 416 aa overlap); and AF143772|AF143772_15 glycosyltransferase gtfB from Mycobacterium avium strain 215 (418 aa), FASTA scores: opt: 1801, E(): 0, (65.2% identity in 417 aa overlap).; Probable glycosyltransferase 1719970 885814 Rv1524 Mycobacterium tuberculosis H37Rv Probable glycosyltransferase NP_216040.1 1718726 D 83332 CDS NP_216041.1 15608663 886470 1720017..1720802 1 NC_000962.3 Rv1525, (MT1576, MTCY19G5.03c), len: 261 aa. Possible wbbL2, rhamnosyl transferase (see citation below),showing weak similarity to several rhamnosyl transferases. Similar to AF105060|AF105060_1 Riftia pachyptila endosymbiont (746 aa), FASTA scores: opt: 183, E(): 0.00013, (35.2% identity in 105 aa overlap).; Possible rhamnosyl transferase WbbL2 1720802 wbbL2 886470 wbbL2 Mycobacterium tuberculosis H37Rv Possible rhamnosyl transferase WbbL2 NP_216041.1 1720017 D 83332 CDS NP_216042.1 15608664 886434 complement(1720780..1722060) 1 NC_000962.3 Rv1526c, (MTCY19G5.02), len: 426 aa. Probable glycosyltransferase, highly similar to G467196 Protein L518_C2_147 from Mycobacterium leprae (421 aa), FASTA scores, opt: 1497, E(): 0, (55.0% identity in 424 aa overlap); similar to G452504 rhamnosyltransferase (24.7% identity in 433 aa overlap); and P96565|U84350 glycosyltransferase GTFE from Amycolatopsis orientalis (408 aa), E(): 3.4e-24, (28.4% identity in 429 aa overlap), also high similarity to Rv1524|MTCY19G5.04c (58.7 % identity in 416 aa overlap).; Probable glycosyltransferase 1722060 886434 Rv1526c Mycobacterium tuberculosis H37Rv Probable glycosyltransferase NP_216042.1 1720780 R 83332 CDS NP_216043.1 15608665 886442 complement(1722083..1728409) 1 NC_000962.3 Rv1527c, (MTV045.01c-MTCY19G5.01), len: 2108 aa. Probable pks5, polyketide synthase, highly similar to many e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 6270,E(): 0, (63.6% identity in 2126 aa overlap).; Probable polyketide synthase Pks5 1728409 pks5 886442 pks5 Mycobacterium tuberculosis H37Rv Probable polyketide synthase Pks5 NP_216043.1 1722083 R 83332 CDS NP_216044.1 15608666 886028 complement(1728953..1729450) 1 NC_000962.3 Rv1528c, (MTV045.02), len: 165 aa. Probable papA4,conserved polyketide synthase (PKS) associated protein; shows some similarity to C-terminal part of hypothetical proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. Z97188|MTCY409_10 Mycobacterium tuberculosis cosmid (468) (37.9% identity in 66 aa overlap); or U00010_11 Mycobacterium leprae cosmid B1170 (35.7% identity in 84 aa overlap). Also similar to Mycobacterium tuberculosis PKS-associated proteins Rv1182, Rv3824c,Rv3820c.; Probable conserved polyketide synthase associated protein PapA4 1729450 papA4 886028 papA4 Mycobacterium tuberculosis H37Rv Probable conserved polyketide synthase associated protein PapA4 NP_216044.1 1728953 R 83332 CDS NP_216045.1 15608667 886432 1729502..1731256 1 NC_000962.3 Rv1529, (MTV045.03), len: 584 aa. Probable fadD24,fatty-acid-AMP synthetase, highly similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA score: (65.6% identity in 582 aa overlap); and many other fatty-acid-CoA synthetases from Mycobacteria e.g. fadD25|MTCY19G5_7 from Mycobacterium tuberculosis (583 aa), FASTA score: (68.7% identity in 584 aa overlap); fadD28|MTCY24G1_8 from Mycobacterium tuberculosis (580 aa), FASTA score: (66.0% identity in 582 aa overlap); NP_301232.1|NC_002677|U00010_6 from Mycobacterium leprae (372 aa), FASTA score: (57.6% identity in 342 aa overlap); FADD23|Rv3826|MTCY409.04c from Mycobacterium tuberculosis (584 aa), FASTA score: (63.2% identity in 584 aa overlap); etc.; Probable fatty-acid-AMP ligase FadD24 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 1731256 fadD24 886432 fadD24 Mycobacterium tuberculosis H37Rv Probable fatty-acid-AMP ligase FadD24 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_216045.1 1729502 D 83332 CDS NP_216046.1 15608668 886426 1731373..1732476 1 NC_000962.3 Rv1530, (MTV045.04), len: 367 aa. Probable adh,alcohol dehydrogenase, zinc-dependent, similar to many e.g. AE0009|AE000958_23 Archaeoglobus fulgidus section 1 (402 aa), FASTA scores: opt: 423, E(): 1.8e-19, (31.7% identity in 341 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature.; Probable alcohol dehydrogenase Adh 1732476 adh 886426 adh Mycobacterium tuberculosis H37Rv Probable alcohol dehydrogenase Adh NP_216046.1 1731373 D 83332 CDS NP_216047.1 15608669 886437 1732473..1733039 1 NC_000962.3 Rv1531, (MTV045.05), len: 188 aa. Conserved protein,similar to Rv0464c|MTV038.08c (190 aa), FASTA scores: E(): 4.8e-10, (30.9% identity in 175 aa overlap).; hypothetical protein 1733039 886437 Rv1531 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216047.1 1732473 D 83332 CDS NP_216048.1 15608670 886422 complement(1733116..1733550) 1 NC_000962.3 Rv1532c, (MTCY07A7A.01c), len: 144 aa. Conserved hypothetical protein, similar to P20378|YPHR_HALHA Hypothetical 15.6 kDa protein from Halobacterium halobium (151 aa), FASTA scores: opt: 152, E():4.5e-05, (30.1% identity in 103 aa overlap).; hypothetical protein 1733550 886422 Rv1532c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216048.1 1733116 R 83332 CDS NP_216049.1 15608671 886424 1733610..1734737 1 NC_000962.3 Rv1533, (MTCY07A7A.02), len: 375 aa. Conserved protein. Similar to 2NPD_NEUCR|Q01284 2-nitropropane dioxygenase precursor (378 aa), fasta scores: opt: 279,E(): 9.1e-11, (31.3% identity in 256 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1894c, Rv0021c, Rv3553, Rv2781c.; hypothetical protein 1734737 886424 Rv1533 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216049.1 1733610 D 83332 CDS NP_216050.1 15608672 886420 1734734..1735411 1 NC_000962.3 Rv1534, (MTCY07A7A.03), len: 225 aa. Probable transcriptional regulator, similar to YCDC_ECOLI|P75899 hypothetical transcriptional regulator from Escherichia coli (212 aa), FASTA scores: opt: 166, E(): 9.8e-05, (24.2% identity in 219 aa overlap). Contains PS01081 Bacterial regulatory proteins, TetR family signature and helix turn helix motif (aa 41-62).; Probable transcriptional regulator 1735411 886420 Rv1534 Mycobacterium tuberculosis H37Rv Probable transcriptional regulator NP_216050.1 1734734 D 83332 CDS NP_216051.1 15608673 886429 1735976..1736212 1 NC_000962.3 Rv1535, (MTCY07A7A.04), len: 78 aa. Unknown protein.; hypothetical protein 1736212 886429 Rv1535 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216051.1 1735976 D 83332 CDS NP_216052.1 15608674 886412 1736519..1739644 1 NC_000962.3 Rv1536, (MTCY48.29c-MTCCY07A7A.05), len: 1041 aa. ileS, Isoleucyl-tRNA synthetase , similar to several e.g. SYIC_YEAST P09436 isoleucyl-tRNA synthetase (1072 aa),FASTA scores: opt: 1447, E(): 0, (37.8% identity in 1072 aa overlap); contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family.; Isoleucyl-tRNA synthetase IleS 1739644 ileS 886412 ileS Mycobacterium tuberculosis H37Rv Isoleucyl-tRNA synthetase IleS NP_216052.1 1736519 D 83332 CDS NP_216053.3 448824759 886414 1739856..1741247 1 NC_000962.3 Rv1537, (MTCY48.28c, MT1589), len: 463 aa. Probable dinX (alternate gene name: dinB1), DNA polymerase IV. Similar to umuC, mucB, samb, and impb (UV protection and mutation) e.g. IMPB_SALTY|P18642 impb protein from Salmonella typhimurium (424 aa), FASTA scores: opt: 386,E(): 1.7e-17, (27.5% identity in 415 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv3056|dinP. Belongs to the DNA polymerase type-Y family.; Probable DNA polymerase IV DinX (pol IV 1) (DNA nucleotidyltransferase (DNA-directed)) 1741247 dinX 886414 dinX Mycobacterium tuberculosis H37Rv Probable DNA polymerase IV DinX (pol IV 1) (DNA nucleotidyltransferase (DNA-directed)) NP_216053.3 1739856 D 83332 CDS NP_216054.1 15608676 886410 complement(1741212..1742192) 1 NC_000962.3 Rv1538c, (MTCY48.27), len: 326 aa. Probable ansA,L-aparaginase, most similar to ASPG_BACLI|P30363 L-asparaginase (322 aa), FASTA scores: opt: 417, E(): 8.8e-19, (30.9% identity in 314 aa overlap). Contains PS00917 Asparaginase / glutaminase active site signature 2.; Probable L-aparaginase AnsA 1742192 ansA 886410 ansA Mycobacterium tuberculosis H37Rv Probable L-aparaginase AnsA NP_216054.1 1741212 R 83332 CDS NP_216055.1 15608677 886408 1742244..1742852 1 NC_000962.3 Rv1539, (MTCY48.26c), len: 202 aa. Probable lspA,lipoprotein signal peptidase (see citation below), similar to several e.g. LSPA_PSEFL|P17942 (170 aa), FASTA scores: opt: 299, E(): 2.6e-12, (38.3% identity in 167 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable lipoprotein signal peptidase LspA 1742852 lspA 886408 lspA Mycobacterium tuberculosis H37Rv Probable lipoprotein signal peptidase LspA NP_216055.1 1742244 D 83332 CDS NP_216056.1 15608678 886404 1742845..1743771 1 NC_000962.3 Rv1540, (MTCY48.25c), len: 308 aa. Member of the yabO/yceC/yfiI family of hypothetical proteins, similar to P44445|YFII_HAEIN hypothetical protein HI0176 from Haemophilus influenzae (324 aa), FASTA scores: opt: 437,E(): 1.2e-22, (33.2% identity in 322 aa overlap). Equivalent to AL049478|MLCL458_13 hypothetical protein from Mycobacterium leprae (308 aa), (89.3% identity in 307 aa overlap). Contains PS01129 hypothetical yabO/yceC/yfiI family signature.; Conserved hypothetical protein member of yabO/yceC/yfiI family 1743771 886404 Rv1540 Mycobacterium tuberculosis H37Rv Conserved hypothetical protein member of yabO/yceC/yfiI family NP_216056.1 1742845 D 83332 CDS NP_216057.1 15608679 886406 complement(1743778..1744371) 1 NC_000962.3 Rv1541c, (MTCY48.24), len: 197 aa. Possible lipoprotein lprI, contains appropriately positioned prokaryotic membrane lipoprotein lipid attachment site (PS0013).; Possible lipoprotein LprI 1744371 lprI 886406 lprI Mycobacterium tuberculosis H37Rv Possible lipoprotein LprI NP_216057.1 1743778 R 83332 CDS NP_216058.1 15608680 886402 complement(1744426..1744836) 1 NC_000962.3 Rv1542c, (MTCY48.23), len: 136 aa. glbN, hemoglobin. Belongs to the protozoan/cyanobacterial globin family. Similar to myoglobins e.g. GLB_PARCA|P15160 myoglobin (hemoglobin) paramecium (116 aa), FASTA scores, opt: 284,E(): 2.1e -13, (35.7% identity in 115 aa overlap). Similar to Mycobacterium tuberculosis hypothetical globin, Rv2470.; Hemoglobin GlbN 1744836 glbN 886402 glbN Mycobacterium tuberculosis H37Rv Hemoglobin GlbN NP_216058.1 1744426 R 83332 CDS NP_216059.1 15608681 886400 1745064..1746089 1 NC_000962.3 Rv1543, (MTCY48.22c), len: 341 aa. Possible fatty-acyl CoA reductase, highly similar to P94129|U77680 fatty acyl-CoA reductase ACR1 from Acinetobacter calcoaceticus (295 aa), FASTA scores: opt: 899, E(): 0,(48.5% identity in 293 aa overlap). Also highly similar to acrA1|Rv3391|MTV004.49|NP_217908.1|NC_000962 fatty acyl-CoA reductase from Mycobacterium tuberculosis (650 aa). Also highly similar to many oxidoreductases short-chain family.; Possible fatty acyl-CoA reductase 1746089 886400 Rv1543 Mycobacterium tuberculosis H37Rv Possible fatty acyl-CoA reductase NP_216059.1 1745064 D 83332 CDS NP_216060.1 15608682 886417 1746094..1746897 1 NC_000962.3 Rv1544, (MTCY48.21), len: 267 aa. Possible ketoacyl reductase, highly similar to Z97179|MLCL383_26 putative oxidoreductase from Mycobacterium leprae (268 aa), FASTA score: (43.0% identity in 270 aa overlap). Also highly similar to others e.g. T29125 ketoacyl reductase homolog from Streptomyces coelicolor (276 aa); NP_470957.1|NC_003212 protein similar to ketoacyl reductases from Listeria innocua (253 aa); HETN_ANASP|P37694 ketoacyl reductase from Anabaena sp. strain PCC 7120 (287 aa), FASTA scores: opt: 379, E(): 7.5e-18, (31.6% identity in 250 aa overlap); etc. And highly similar to many oxidoreductases short-chain family. Also highly similar to Rv2509 from Mycobacterium tuberculosis (268 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Possible ketoacyl reductase 1746897 886417 Rv1544 Mycobacterium tuberculosis H37Rv Possible ketoacyl reductase NP_216060.1 1746094 D 83332 CDS NP_216061.1 15608683 886398 1746919..1747146 1 NC_000962.3 Rv1545, (MTCY48.20), len: 75 aa. Hypothetical unknown protein.; Hypothetical protein 1747146 886398 Rv1545 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216061.1 1746919 D 83332 CDS NP_216062.1 15608684 886394 1747195..1747626 1 NC_000962.3 Rv1546, (MTCY48.19c), len: 143 aa. Conserved protein, similar to O05902|Rv0910|MTCY21C12.04 Hypothetical protein from Mycobacterium tuberculosis (144 aa), FASTA scores: E(): 5e-30, (37.3% identity in 142 aa overlap).; hypothetical protein 1747626 886394 Rv1546 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216062.1 1747195 D 83332 CDS NP_216063.1 15608685 886392 1747694..1751248 1 NC_000962.3 Rv1547, (MTCY48.18c), len: 1184 aa. Probable dnaE1,DNA polymerase III, alpha chain (see citation below),similar to many e.g. DP3A_ECOLI|P10443 dna polymerase III,alpha chain (1160 aa), FASTA scores: opt: 1789, E(): 0,(36.5% identity in 1193 aa overlap). Also similar to M. tuberculosis, DnaE2|Rv3370c. Belongs to DNA polymerase type-C family, DNAE subfamily.; Probable DNA polymerase III (alpha chain) DnaE1 (DNA nucleotidyltransferase) 1751248 dnaE1 886392 dnaE1 Mycobacterium tuberculosis H37Rv Probable DNA polymerase III (alpha chain) DnaE1 (DNA nucleotidyltransferase) NP_216063.1 1747694 D 83332 CDS YP_177817.1 57116882 886384 complement(1751297..1753333) 1 NC_000962.3 Rv1548c, (MTCY48.17), len: 678 aa. PPE21, Member of the Mycobacterium tuberculosis PPE family, similar to several e.g. YHS6_MYCTU|P42611 hypothetical 50.6 kDa protein in hsp65 3' region (517 aa), FASTA scores: opt:1142, E(): 0, (40.6% identity in 616 aa overlap); also similar to MTCY31.06c (54.9% identity in 381 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).; PPE family protein PPE21 1753333 PPE21 886384 PPE21 Mycobacterium tuberculosis H37Rv PPE family protein PPE21 YP_177817.1 1751297 R 83332 CDS YP_177818.1 57116883 886386 1753510..1754037 1 NC_000962.3 Rv1549, (MTCY48.16c), len: 175 aa. Possible fadD11.1, fatty-acid-CoA synthetase, similar to the N-terminus of many fatty-acid CoA synthetases e.g. NP_147860.1|NC_000854 long-chain-fatty-acid--CoA ligase from Aeropyrum pernix (651 aa); P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 from Solanum tuberosum (Potato) (545 aa), FASTA scores: opt: 168, E(): 4.4e-06, (30.4% identity in 112 aa overlap); etc. Possible frameshift with respect to next ORF Rv1550|MTCY48.15c but we can find no sequence error to account for this. Note that previously known as fadD11'.; Possible fatty-acid-CoA ligase FadD11.1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 1754037 fadD11.1 886386 fadD11.1 Mycobacterium tuberculosis H37Rv Possible fatty-acid-CoA ligase FadD11.1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) YP_177818.1 1753510 D 83332 CDS NP_216066.1 15608688 886380 1753716..1755431 1 NC_000962.3 Rv1550, (MTCY48.15c), len: 571 aa. Probable fadD11,fatty-acid-CoA synthetase, similar, except in N-terminus,to many e.g. SC6A5.39|T35430 probable long-chain-fatty-acid--CoA ligase from Streptomyces coelicolor (612 aa); NP_301672.1|NC_002677 putative long-chain-fatty-acid-CoA ligase from Mycobacterium leprae (600 aa); P44446|LCFH_HAEIN putative long-chain-fatty-acid-CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: opt: 762, E(): 2.3e-38,(34.4% identity in 436 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. Possible frameshift with respect to previous ORF Rv1549|MTCY48.16c but we can find no sequence error to account for this.; Probable fatty-acid-CoA ligase FadD11 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 1755431 fadD11 886380 fadD11 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD11 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_216066.1 1753716 D 83332 CDS NP_216067.1 15608689 886382 1755445..1757310 1 NC_000962.3 Rv1551, (MT1601, MTCY48.14c), len: 621 aa. Possible plsB1, acyltransferase, similar to PLSB_HAEIN|P44857 glycerol-3-phosphate acyltransferase from Haemophilus influenzae (810 aa), FASTA scores: opt: 434, E(): 6.2e-22,(27.6% identity in 395 aa overlap). Also similar to Rv2482c|plsB2 Probable glycerol-3-phosphate acyltransferase from Mycobacterium tuberculosis (789 aa).; Possible acyltransferase PlsB1 1757310 plsB1 886382 plsB1 Mycobacterium tuberculosis H37Rv Possible acyltransferase PlsB1 NP_216067.1 1755445 D 83332 CDS NP_216068.1 15608690 886376 1757681..1759432 1 NC_000962.3 Rv1552, (MTCY48.13c), len: 583 aa. Probable frdA,fumarate reductase, flavoprotein subunit, highly similar to others e.g. P00363|FRDA_ECOLI fumarate reductase flavoprotein subunit from Escherichia coli strain K12 (601 aa), FASTA scores: opt: 2102, E(): 0, (54.7% identity in 585 aa overlap); NP_232284.1|NC_002505 fumarate reductase,flavoprotein subunit from Vibrio cholerae (602 aa); frdA|NP_438995.1|NC_000907 fumarate reductase, flavoprotein subunit from Haemophilus influenzae (599 aa); etc. Contains PS00504 Fumarate reductase / succinate dehydrogenase FAD-binding site. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB),and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD).; Probable fumarate reductase [flavoprotein subunit] FrdA (fumarate dehydrogenase) (fumaric hydrogenase) 1759432 frdA 886376 frdA Mycobacterium tuberculosis H37Rv Probable fumarate reductase [flavoprotein subunit] FrdA (fumarate dehydrogenase) (fumaric hydrogenase) NP_216068.1 1757681 D 83332 CDS NP_216069.1 15608691 886378 1759435..1760178 1 NC_000962.3 Rv1553, (MTCY48.12c), len: 247 aa. Probable frdB,fumarate reductase, iron-sulfur subunit, highly similar to others e.g. P00364|FRDB_ECOLI fumarate reductase iron-sulfur protein from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 846, E(): 0, (50.0% identity in 242 aa overlap); P20921|FRDB_PROVU fumarate reductase iron-sulfur protein from Proteus vulgaris (245 aa); G64097 fumarate reductase iron-sulfur protein from Haemophilus influenzae (276 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD).; Probable fumarate reductase [iron-sulfur subunit] FrdB (fumarate dehydrogenase) (fumaric hydrogenase) 1760178 frdB 886378 frdB Mycobacterium tuberculosis H37Rv Probable fumarate reductase [iron-sulfur subunit] FrdB (fumarate dehydrogenase) (fumaric hydrogenase) NP_216069.1 1759435 D 83332 CDS NP_216070.1 15608692 886371 1760175..1760555 1 NC_000962.3 Rv1554, (MTCY48.11c), len: 126 aa. Probable frdC,fumarate reductase, membrane-anchor subunit, highly similar to others e.g. P03805|FRDC_ECOLI fumarate reductase 15 kDa hydrophobic protein from Escherichia coli strain K12 (131 aa), FASTA scores, opt: 268, E(): 3.9e-10, (31.1% identity in 122 aa overlap); NP_458780.1|NC_003198 fumarate reductase complex subunit C; membrane anchor polypeptide from Salmonella enterica subsp. enterica serovar Typhi (131 aa); P20923|FRDC_PROVU fumarate reductase 15 kDa hydrophobic protein from Proteus vulgaris (131 aa); etc. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD).; Probable fumarate reductase [membrane anchor subunit] FrdC (fumarate dehydrogenase) (fumaric hydrogenase) 1760555 frdC 886371 frdC Mycobacterium tuberculosis H37Rv Probable fumarate reductase [membrane anchor subunit] FrdC (fumarate dehydrogenase) (fumaric hydrogenase) NP_216070.1 1760175 D 83332 CDS NP_216071.1 15608693 886389 1760552..1760929 1 NC_000962.3 Rv1555, (MTCY48.10c), len: 125 aa. Probable frdD,fumarate reductase, membrane-anchor subunit, similar to others e.g. P03806|FRDD_ECOLI fumarate reductase 13 kDa hydrophobic protein from Escherichia coli strain K12 (119 aa), FASTA scores: opt: 212, E(): 4.4e-08, (36.8% identity in 106 aa overlap); etc. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB),and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD).; Probable fumarate reductase [membrane anchor subunit] FrdD (fumarate dehydrogenase) (fumaric hydrogenase) 1760929 frdD 886389 frdD Mycobacterium tuberculosis H37Rv Probable fumarate reductase [membrane anchor subunit] FrdD (fumarate dehydrogenase) (fumaric hydrogenase) NP_216071.1 1760552 D 83332 CDS NP_216072.1 15608694 886367 1760997..1761605 1 NC_000962.3 Rv1556, (MTCY48.09c), len: 202 aa. Possible regulatory protein, similar to X86780|SHGCPIR2|g987088 orfY, regulator of antibiotic transport complexes from Streptomyces hygroscopicus (204 aa), FASTA score: opt: 251,E(): 1.7e-10, (33.8% identity in 201 aa overlap) and others.; Possible regulatory protein 1761605 886367 Rv1556 Mycobacterium tuberculosis H37Rv Possible regulatory protein NP_216072.1 1760997 D 83332 CDS NP_216073.1 15608695 886033 1761744..1762937 1 NC_000962.3 Rv1557, (MTCY48.08c), len: 397 aa. Probable mmpL6,conserved transmembrane transport protein (see citations below). Member of RND superfamily, with strong similarity to C-terminal part of members of large Mycobacterial membrane protein family belonging to RND superfamily including: mmpL1, mmpL2, mmpL3, etc. Probably truncated (see Brosch et al., 2002). Belongs to the MmpL family.; Probable conserved transmembrane transport protein MmpL6 1762937 mmpL6 886033 mmpL6 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL6 NP_216073.1 1761744 D 83332 CDS NP_216074.1 15608696 886363 1762947..1763393 1 NC_000962.3 Rv1558, (MTCY48.07c), len: 148 aa. Conserved protein, similar to other Mycobacterial tuberculosis proteins e.g. P71854|MTCY03C7.09c|Rv3547 (151 aa), FASTA scores opt: 330, E(): 9.1e-17, (39.7% identity in 151 aa overlap); also Q11057|Rv1261c (149 aa), and O53328|Rv3178 (119 aa). Similar also to AF072709|AF072709_5 Hypothetical protein with a new amplifiable element AUD4 from Streptomyces lividans (149 aa), FASTA scores: opt: 695,E(): 0, (69.1% identity in 149 aa overlap).; hypothetical protein 1763393 886363 Rv1558 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216074.1 1762947 D 83332 CDS NP_216075.1 15608697 886365 1763428..1764717 1 NC_000962.3 Rv1559, (MTCY48.06c), len: 429 aa. Probable ilvA,threonine dehydratase, biosynthetic protein, similar to several e.g. THD1_CORGL|Q04513 threonine dehydratase biosynthetic (436 aa), FASTA scores: opt: 1694, E(): 0,(61.9% identity in 415 aa overlap). Contains PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site.; Probable threonine dehydratase IlvA 1764717 ilvA 886365 ilvA Mycobacterium tuberculosis H37Rv Probable threonine dehydratase IlvA NP_216075.1 1763428 D 83332 CDS NP_216076.1 15608698 886359 1764755..1764973 1 NC_000962.3 Rv1560, (MTCY48.05c), len: 72 aa. Possible vapB11,antitoxin, part of toxin-antitoxin (TA) operon with Rv1561 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Part of a Mycobacterial tuberculosis family of proteins e.g. Q10848|Rv2009|MTCY39.08c (80 aa), FASTA score: (54.4% identity in 68 aa overlap); Q10799|Rv2871|MTCY274.02 (85 aa); O50456|Rv1241|MTV006.13 (86 aa),O06243|Rv2132|MTCY270.36C (76 aa); etc.; Possible antitoxin VapB11 1764973 vapB11 886359 vapB11 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB11 NP_216076.1 1764755 D 83332 CDS NP_216077.1 15608699 886361 1764979..1765383 1 NC_000962.3 Rv1561, (MTCY48.04c), len: 134 aa. Possible vapC11,toxin, part of toxin-antitoxin (TA) operon with Rv1560,contains PIN domain, (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others from Mycobacterium tuberculosis e.g. Q10847|Rv2010|MTCY39.07c (132 aa), FASTA scores: (37.0% identity in 127 aa overlap); and O06566|Rv1114|MTCY22G8.03 (124 aa).; Possible toxin VapC11 1765383 vapC11 886361 vapC11 Mycobacterium tuberculosis H37Rv Possible toxin VapC11 NP_216077.1 1764979 D 83332 CDS YP_177819.1 57116884 886355 complement(1765400..1767142) 1 NC_000962.3 Rv1562c, (MTCY48.03), len: 580 aa. TreZ (previously called glgZ), Maltooligosyltrehalose trehalohydrolase,confirmed biochemically (see citation below). Similar to Q44316|D63343 TREZ maltooligosyl trehalose trehalohydrolase from arthrobacter SP (598 aa), FASTA scores: opt: 2071,E(): 0, (52.2% identity in 582 aa overlap); also similar to 1,4-alpha-glucan branching enzymes e.g. GLGB_BACST|P30538 (639 aa), FASTA scores: opt: 313, E(): 3.8e-13, (27.5% identity in 462 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv1326c|glgB, and Rv1563c treY (previously glgY).; Maltooligosyltrehalose trehalohydrolase TreZ 1767142 treZ 886355 treZ Mycobacterium tuberculosis H37Rv Maltooligosyltrehalose trehalohydrolase TreZ YP_177819.1 1765400 R 83332 CDS YP_177820.1 57116885 886357 complement(1767135..1769432) 1 NC_000962.3 Rv1563c, (MTCY48.02), len: 765 aa. TreY (previously called glgY), maltooligosyl trehalose synthase, confirmed biochemically (see citation below). Strong similarity to Q44315|63343 trey maltooligosyl trehalose synthase from arthrobacter SP (775 aa), fasta scores: opt: 1953, E(): 0; (46.0% identity in 789 aa overlap). Some similarity to alpha-amylases and to MTCY48.03 (30.2% identity in 215 aa overlap). May catalyse conversion of maltodextrins to maltooligosyl trehaloses. Also similar to Mycobacterium tuberculosis glgB (Rv1326c), treZ (Rv1562c).; Maltooligosyltrehalose synthase TreY 1769432 treY 886357 treY Mycobacterium tuberculosis H37Rv Maltooligosyltrehalose synthase TreY YP_177820.1 1767135 R 83332 CDS YP_177821.1 57116886 886353 complement(1769436..1771601) 1 NC_000962.3 Rv1564c, (MTCY48.01), len: 721 aa. Probable treX (previously called glgX), Maltooligosyltrehalose synthase. Strong similarity to D83245|g1890053 treX, glycogen debranching enzyme (glgX) from Sulfolobus acidocaldarius (713 aa), FASTA score: opt: 2396, E(): 0, (48.4% identity in 709 aa overlap); similar to GLGX_HAEIN|P45178 glycogen operon protein glgx (659 aa), FASTA scores: opt: 1512, E(): 0, (42.3% identity in 645 aa overlap).; Probable maltooligosyltrehalose synthase TreX 1771601 treX 886353 treX Mycobacterium tuberculosis H37Rv Probable maltooligosyltrehalose synthase TreX YP_177821.1 1769436 R 83332 CDS NP_216081.1 15608703 886351 complement(1771640..1773829) 1 NC_000962.3 Rv1565c, (MTCY336.38), len: 729 aa. Conserved hypothetical membrane protein, some similarity to O05402 hypothetical 72.2 kDa protein from Bacillus subtilis (634 aa), FASTA results: opt: 384, E(): 4.8e-17, (29.1% identity in 378 aa overlap); and to Y392_HAEIN|P43993 hypothetical protein hi0392 from H. influenzae (245 aa), FASTA results: opt: 265, E(): 5.5e-10, (28.3% identity in 247 aa overlap). C-terminal half equivalent to AL049478|MLCL458_19 (274 aa) (78.5% identity in 274 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0111,Rv0228, Rv1254, Rv0517. N-terminal half hydrophobic.; hypothetical protein 1773829 886351 Rv1565c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216081.1 1771640 R 83332 CDS NP_216082.1 15608704 886347 complement(1773928..1774620) 1 NC_000962.3 Rv1566c, (MTCY336.37), len: 230 aa. Possible inv protein, probably exported as has QQAPV repeats at C-terminus. Similar to Q49634 inv protein from Mycobacterium leprae (246 aa), FASTA scores: opt: 957, E(): 0, (70.0% identity in 207 aa overlap); also to putative invasins 1,2 (O07390, O07391) from Mycobacterium avium. Slightly similar to C-terminus of P60_LISMO|P21171 Listeria invasion-associated protein p60 precursor. Also similar to Mycobacterium tuberculosis p60 homologues Rv1477, Rv1478,Rv0024, Rv2190c. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible Inv protein 1774620 886347 Rv1566c Mycobacterium tuberculosis H37Rv Possible Inv protein NP_216082.1 1773928 R 83332 CDS NP_216083.1 15608705 886349 complement(1774860..1775144) 1 NC_000962.3 Rv1567c, (MTCY336.36), len: 94 aa. Probable membrane protein.; Probable hypothetical membrane protein 1775144 886349 Rv1567c Mycobacterium tuberculosis H37Rv Probable hypothetical membrane protein NP_216083.1 1774860 R 83332 CDS NP_216084.1 15608706 886343 1775392..1776705 1 NC_000962.3 Rv1568, (MTCY336.35c), len: 437 aa. bioA,adenosylmethionine-8-amino-7-oxononanoate aminotransferase , equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below). Highly similar to BIOA_MYCLE|P4548 from Mycobacterium leprae (436 aa), FASTA results: opt: 2534, E(): 0, (85.1% identity in 436 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTCY227.12c (449 aa), FASTA score: E(): 3.5e-16, (29.5% identity in 421 aa overlap). Contains aminotransferases class-III pyridoxal-phosphate attachment site (PS00600). Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases.; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA 1776705 bioA 886343 bioA Mycobacterium tuberculosis H37Rv Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA NP_216084.1 1775392 D 83332 CDS YP_177822.1 57116887 886345 1776702..1777862 1 NC_000962.3 Rv1569, (MTCY336.34c), len: 386 aa. Probable bioF1,8-amino-7-oxononanoate synthase, highly similar to BIOF_MYCLE|P45487 from Mycobacterium leprae (385 aa), FASTA results: opt: 1971, E(): 0, (80.1% identity in 381 aa overlap). Also similar to BIOF2|Rv0032|MTCY10H4.32 possible 8-amino-7-oxononanoate synthase from Mycobacterium tuberculosis (771 aa), FASTA score: E(): 5.5e-29, (37.4% identity in 393 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases.; Probable 8-amino-7-oxononanoate synthase BioF1 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) 1777862 bioF1 886345 bioF1 Mycobacterium tuberculosis H37Rv Probable 8-amino-7-oxononanoate synthase BioF1 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) YP_177822.1 1776702 D 83332 CDS NP_216086.1 15608708 886338 1777859..1778539 1 NC_000962.3 Rv1570, (MTCY336.33c), len: 226 aa. bioD,dethiobiotin synthetase. Similar to many e.g. BIOD_MYCLE|P45486 from Mycobacterium leprae (223 aa), FASTA results: opt: 1059, E(): 0, (74.8% identity in 222 aa overlap). Belongs to the dethiobiotin synthetase family.; Dethiobiotin synthetase BioD 1778539 bioD 886338 bioD Mycobacterium tuberculosis H37Rv Dethiobiotin synthetase BioD NP_216086.1 1777859 D 83332 CDS NP_216087.1 15608709 886341 1778539..1779048 1 NC_000962.3 Rv1571, (MTCY336.32c), len: 169 aa. Conserved protein, similar at N-terminal region to Q49625|LEPB1170_C3_227 hypothetical protein from Mycobacterium leprae (104 aa), FASTA results: opt: 473,E(): 3.9e-24, (74.5% identity in 102 aa overlap). Identical to O06619|AF041819|AF041819_6 Mycobacterium bovis BCG (169 aa).; hypothetical protein 1779048 886341 Rv1571 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216087.1 1778539 D 83332 CDS NP_216088.2 57116888 886333 complement(1779194..1779298) 1 NC_000962.3 Rv1572c, (MTCY336.31B), len: 34 aa. Partial ORF,part of REP13E12 repeat element; 3' end of Rv1587c (MTCY336.17) after phage-like element (see citation below). Similar to C-terminal ends of other REP13E12 repeat elements e.g. Rv1148, Rv1945, Rv3467, etc. Length extended since first submission (+7 aa). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 1779298 886333 Rv1572c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216088.2 1779194 R 83332 CDS NP_216089.1 15608711 886337 1779314..1779724 1 NC_000962.3 Rv1573, (MTCY336.31c), len: 136 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1779724 886337 Rv1573 Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216089.1 1779314 D 83332 CDS NP_216090.1 15608712 886331 1779930..1780241 1 NC_000962.3 Rv1574, (MTCY336.30), len: 103 aa. Probable phiRV1 phage related protein (see citation below); some similarity to N-terminus of Rv1575|MTCY441.17 Probable phiRV1 phage protein (166 aa), E(): 1.5e-06; and Rv2647|MTCY336.29c Probable phiRV2 phage protein, E(): 3.5e-05. Helix turn helix motif present at aa 14-35 (+3.61 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage related protein 1780241 886331 Rv1574 Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage related protein NP_216090.1 1779930 D 83332 CDS NP_216091.2 57116889 886335 1780199..1780699 1 NC_000962.3 Rv1575, (MTCY336.29c), len: 166 aa. Probable phiRV1 phage protein (see citation below), showing similarity in N-terminal part to Rv1574|MTCY336.30c Probable phiRV1 phage protein (103 aa), FASTA score: opt: 375, E(): 3.8e-16,(60.2% identity in 103 aa overlap); and Rv2647 Probable phiRV2 phage protein. Start changed since first submission (+49 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1780699 886335 Rv1575 Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216091.2 1780199 D 83332 CDS NP_216092.1 15608714 886327 complement(1780643..1782064) 1 NC_000962.3 Rv1576c, (MTCY336.28), len: 473 aa. Probable phiRV1 phage protein (capsid subunit) (see citation below). Highly similar to hypothetical Mycobacterium tuberculosis protein Rv2650c|MTCY441.19 phiRV2 phage related protein, FASTA scores: opt: 2782, E(): 0, (89.1% identity in 468 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1782064 886327 Rv1576c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216092.1 1780643 R 83332 CDS NP_216093.1 15608715 886329 complement(1782072..1782584) 1 NC_000962.3 Rv1577c, (MTCY336.27), len: 170 aa. Probable phiRv1 phage protein (prohead protease) (see citation below). Highly similar to hypothetical protein Rv2651c|MTCY441.20c phiRV2 prohead protease, FASTA scores: E(): 0, (89.3% identity in 169 aa overlap). Some similarity to VP4_BPHK7|P49860 putative bacteriophage HK97 prohead protease (gp4) (225 aa), FASTA results: opt: 176, E(): 1.3e-05, (27.3% identity in 165 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1782584 886329 Rv1577c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216093.1 1782072 R 83332 CDS NP_216094.1 15608716 886322 complement(1782758..1783228) 1 NC_000962.3 Rv1578c, (MTCY336.26), len: 156 aa. Probable phiRv1 phage protein (terminase) (see citation below), highly similar to Rv2652c|MTCY441.21c phiRV2 phage protein from Mycobacterium tuberculosis, FASTA scores: E(): 4.8e-22,(48.1% identity in 156 aa overlap). Also similar to X65555|ARP3COS_1 hypothetical protein (cos site) -actinophage RP3 (210 aa), FASTA scores: opt: 373, E(): 6.5e-17, (50.0% identity in 114 aa overlap). Contains MIP family signature (PS00221). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable PhiRv1 phage protein 1783228 886322 Rv1578c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216094.1 1782758 R 83332 CDS NP_216095.1 15608717 886369 complement(1783309..1783623) 1 NC_000962.3 Rv1579c, (MTCY336.25), len: 104 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1783623 886369 Rv1579c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216095.1 1783309 R 83332 CDS NP_216096.1 15608718 886313 complement(1783620..1783892) 1 NC_000962.3 Rv1580c, (MTCY336.24), len: 90 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1783892 886313 Rv1580c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216096.1 1783620 R 83332 CDS NP_216097.1 15608719 886318 complement(1783906..1784301) 1 NC_000962.3 Rv1581c, (MTCY336.23), len: 131 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1784301 886318 Rv1581c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216097.1 1783906 R 83332 CDS NP_216098.1 15608720 886311 complement(1784497..1785912) 1 NC_000962.3 Rv1582c, (MTCY336.22), len: 471 aa. Probable phiRv1 phage protein (see citation below). N-terminus is similar to C-terminus of Q38030 ORF9 Bacteriophage phi-C31 (519 aa), FASTA scores: opt: 331, E(): 6.5e-15, (28.5% identity in 235 aa overlap); and C-terminus to whole of Q38031 ORF10 of Bacteriophage phi-C31 (202 aa), FASTA scores: opt: 353,E(): 1e-16, (31.1% identity in 190 aa overlap). Also similar to part of AB016282|AB016282_42 Bacteriophage phi-105 (806 aa), FASTA scores: opt: 790, E(): 0, (32.7% identity in 459 aa overlap). Similarity to other phage proteins described as putative DNA-polymerase or DNA-primase. Also slightly similar to MTCY441.24c, FASTA scores: E(): 0.0055, (36.0% identity in 75 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1785912 886311 Rv1582c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216098.1 1784497 R 83332 CDS NP_216099.1 15608721 886315 complement(1785912..1786310) 1 NC_000962.3 Rv1583c, (MTCY336.21), len: 132 aa. Probable phiRv1 phage protein (see citation below), highly similar to Rv2656c|MTCY441.25c phiRV2 phage protein (130 aa), FASTA score: E(): 1.3e-33, (81.7% identity in 131 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable PhiRv1 phage protein 1786310 886315 Rv1583c Mycobacterium tuberculosis H37Rv Probable PhiRv1 phage protein NP_216099.1 1785912 R 83332 CDS NP_216100.1 15608722 886307 complement(1786307..1786528) 1 NC_000962.3 Rv1584c, (MTCY336.20), len: 73 aa. Possible phiRv1 phage protein (putative excisionase) (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible PhiRv1 phage protein 1786528 886307 Rv1584c Mycobacterium tuberculosis H37Rv Possible PhiRv1 phage protein NP_216100.1 1786307 R 83332 CDS NP_216101.1 15608723 886309 complement(1786584..1787099) 1 NC_000962.3 Rv1585c, (MTCY336.19), len: 171 aa. Possible phage phiRv1 protein (see Hatfull 2000). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible phage PhiRv1 protein 1787099 886309 Rv1585c Mycobacterium tuberculosis H37Rv Possible phage PhiRv1 protein NP_216101.1 1786584 R 83332 CDS NP_216102.1 15608724 886305 complement(1787096..1788505) 1 NC_000962.3 Rv1586c, (MTCY336.18), len: 469 aa. Probable phiRv1 integrase, possibly member of the serine family of recombinases (see citation below), similar to several bacteriophage integrases e.g. Q37839 ORF469 protein from Bacteriophage R4 (469 aa), FASTA scores: opt: 623, E(): 1.6e-29, (31.1% identity in 482 aa overlap); and Bacteriophage TP901-1. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable PhiRv1 integrase 1788505 886305 Rv1586c Mycobacterium tuberculosis H37Rv Probable PhiRv1 integrase NP_216102.1 1787096 R 83332 CDS NP_216103.2 57116890 886303 complement(1788162..1789163) 1 NC_000962.3 Rv1587c, (MTCY336.17), len: 333 aa. Partial REP13E12 repeat protein (see citation below), nearly identical (but has been interrupted by phiRv1 prophage) to Q50655|MTCY251.13c|Rv0094c hypothetical 34.6 kDa protein from M. tuberculosis (317 aa), FASTA results: opt: 1511,E(): 1.1e-84, (97.75% identity in 224 aa overlap). Codon usage suggests that translation may involve frameshifting of Rv1588c mRNA in poly_C stretch into reading frame of Rv1587c. 3' end found in Rv1572c. Length extended since first submission (+115 aa). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Partial REP13E12 repeat protein 1789163 886303 Rv1587c Mycobacterium tuberculosis H37Rv Partial REP13E12 repeat protein NP_216103.2 1788162 R 83332 CDS NP_216104.1 15608726 886325 complement(1789168..1789836) 1 NC_000962.3 Rv1588c, (MTCY336.16), len: 222 aa. Partial REP13E12 repeat protein (see citation below), nearly identical to ORF's in other Rep13E12 repeats, including Rv0095c|MTCY251.14c|Y05E_MYCTU|Q10891 hypothetical 15.4 kd protein cy251.14 from Mycobacterium tuberculosis (136 aa),FASTA results: opt: 613, E(): 9.9e-29, (86.5% identity in 111 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Partial REP13E12 repeat protein 1789836 886325 Rv1588c Mycobacterium tuberculosis H37Rv Partial REP13E12 repeat protein NP_216104.1 1789168 R 83332 CDS NP_216105.1 15608727 886301 1790284..1791333 1 NC_000962.3 Rv1589, (MTCY336.15c), len: 349 aa. Probable bioB,biotin synthetase O06601. Highly similar to BIOB_MYCLE|P46715 BioB from Mycobacterium leprae (345 aa),FASTA results: opt: 1982, E(): 0, (86.5% identity in 349 aa overlap). Identical to AF041819|AF041819_9 bioB from Mycobacterium bovis BCG (349 aa).; Probable biotin synthetase BioB 1791333 bioB 886301 bioB Mycobacterium tuberculosis H37Rv Probable biotin synthetase BioB NP_216105.1 1790284 D 83332 CDS NP_216106.1 15608728 886292 1791334..1791573 1 NC_000962.3 Rv1590, (MTCY336.14c), len: 79 aa. Conserved hypothetical protein, similar to Q49616|LEPB1170_C1_162|YF90_MYCLE from Mycobacterium leprae (80 aa), FASTA scores: opt: 368, E(): 1.7e-21,Smith-Waterman score: 368, (67.1% identity in 73 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1791573 886292 Rv1590 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216106.1 1791334 D 83332 CDS NP_216107.1 15608729 886295 1791570..1792235 1 NC_000962.3 Rv1591, (MTCY336.13c), len: 221 aa. Probable transmembrane protein, similar to Q49626|LEPB1170_C3_229|YF91_MYCLE Hypothetical Mycobacterium leprae protein (198 aa), FASTA results: opt: 802, E(): 0, (63.8% identity in 188 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable transmembrane protein 1792235 886295 Rv1591 Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_216107.1 1791570 D 83332 CDS NP_216108.1 15608730 886287 complement(1792400..1793740) 1 NC_000962.3 Rv1592c, (MTCY336.12), len: 446 aa. Conserved hypothetical protein, some similarity to Q49629|B1170_F1_46 from Mycobacterium leprae (132 aa), FASTA results: opt: 332, E(): 4.5e-14, (56.3% identity in 87 aa overlap). Nearly identical to truncated Mycobacterium bovis BCG protein (148 aa) AF041819|AF041819_11.; hypothetical protein 1793740 886287 Rv1592c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216108.1 1792400 R 83332 CDS NP_216109.1 15608731 886289 complement(1793997..1794707) 1 NC_000962.3 Rv1593c, (MTCY336.11), len: 236 aa. Conserved protein, highly similar to Q49628|B1170_F1_44 from Mycobacterium leprae (286 aa), FASTA scores: opt: 1304, E (): 0, (85.4% identity in 233 aa overlap); similar to several putative DNA hydrolases e.g. Q9S233|SCI51.07C from Streptomyces coelicolor (239 aa), FASTA scores: opt: 415,E(): 4.6e-20, (34.8% identity in 221 aa overlap); also similar to P74291|SLR1690 hypothetical protein from synechocystis (261 aa), FASTA scores: opt: 228, E(): 1.4e-17, (31.5% identity in 213 aa overlap).; hypothetical protein 1794707 886289 Rv1593c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216109.1 1793997 R 83332 CDS NP_216110.1 15608732 886283 1794756..1795805 1 NC_000962.3 Rv1594, (MTCY336.10c), len: 349 aa. Probable nadA,quinolinate synthetase. Similar to many e.g. Q49622 NADA from Mycobacterium leprae (368 aa), FASTA results: opt: 1994, E(): 0, (84.4% identity in 352 aa overlap).; Probable quinolinate synthetase NadA 1795805 nadA 886283 nadA Mycobacterium tuberculosis H37Rv Probable quinolinate synthetase NadA NP_216110.1 1794756 D 83332 CDS NP_216111.1 15608733 886285 1795805..1797388 1 NC_000962.3 Rv1595, (MTCY336.09c), len: 527 aa. Probable nadB,L-aspartate oxidase. Similar to many e.g. Q49617 L-aspartate oxidase (quinolinate synthetase) from Mycobacterium leprae (424 aa), FASTA results: opt: 2152,E(): 0, (82.0% identity in 400 aa overlap). Also shows some similarity to Rv1552 frdA from Mycobacterium tuberculosis (583 aa), FASTA results: E(): 1e-10, (35.3% identity in 566 aa overlap). Heterodimer. The quinolinate synthetase complex consists of the two enzymes quinolinate synthetase a and B.; Probable L-aspartate oxidase NadB 1797388 nadB 886285 nadB Mycobacterium tuberculosis H37Rv Probable L-aspartate oxidase NadB NP_216111.1 1795805 D 83332 CDS NP_216112.1 15608734 886281 1797388..1798245 1 NC_000962.3 Rv1596, (MTCY336.08c), len: 285 aa. Probable nadC,nicotinate-nucleotide pyrophosphatase O06594. Similar to many e.g. ADC_MYCLE|P46714 from Mycobacterium leprae (284 aa), FASTA results: opt: 1418, E(): 0,(79.2% identity in 283 aa overlap). Belongs to the NADC/MODD family.; Probable nicotinate-nucleotide pyrophosphatase NadC 1798245 nadC 886281 nadC Mycobacterium tuberculosis H37Rv Probable nicotinate-nucleotide pyrophosphatase NadC NP_216112.1 1797388 D 83332 CDS NP_216113.1 15608735 886297 1798294..1799052 1 NC_000962.3 Rv1597, (MTCY336.07c), len: 252 aa. Hypothetical unknown protein.; Hypothetical protein 1799052 886297 Rv1597 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216113.1 1798294 D 83332 CDS NP_216114.1 15608736 886324 complement(1799073..1799483) 1 NC_000962.3 Rv1598c, (MTCY336.06), len: 136 aa. Conserved protein, some similarity to O06389|Rv0523c|MTCY25D10.02 from Mycobacterium tuberculosis (131 aa), FASTA scores: E(): 2.2e-09, (38.4% identity in 99 aa overlap); and P95144|MTCY359.02|Rv1871c (129 aa).; hypothetical protein 1799483 886324 Rv1598c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216114.1 1799073 R 83332 CDS NP_216115.1 15608737 886277 1799583..1800899 1 NC_000962.3 Rv1599, (MTCY336.05c), len: 438 aa. Probable hisD,histidinol dehydrogenase (see citation below) O08396. Similar to many e.g. HISX_MYCSM|P28736 from Mycobacterium smegmatis (445 aa), FASTA results: opt: 2356, E(): 0,(83.1% identity in 437 aa overlap). Contains histidinol dehydrogenase signature (PS00611).; Probable histidinol dehydrogenase HisD (HDH) 1800899 hisD 886277 hisD Mycobacterium tuberculosis H37Rv Probable histidinol dehydrogenase HisD (HDH) NP_216115.1 1799583 D 83332 CDS YP_177823.1 57116891 886298 1800896..1802038 1 NC_000962.3 Rv1600, (MTCY336.04c), len: 380 aa. Probable hisC1,histidinol-phosphate aminotransferase O06591. Similar to many e.g. HIS8_STRCO|P16246 from Streptomyces coelicolor (369 aa), FASTA results: opt: 1353, E(): 0, (59.0% identity in 356 aa overlap). Some similarity to other Mycobacterium tuberculosis aminotransferases e.g. Rv3772|MTCY13D12.06,FASTA results: E(): 7.4e-25, (33.7% identity in 365 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. Note that previously known as hisC.; Probable histidinol-phosphate aminotransferase HisC1 1802038 hisC1 886298 hisC1 Mycobacterium tuberculosis H37Rv Probable histidinol-phosphate aminotransferase HisC1 YP_177823.1 1800896 D 83332 CDS NP_216117.1 15608739 886274 1802035..1802667 1 NC_000962.3 Rv1601, (MTCY336.03c), len: 210 aa. Probable hisB,imidazole glycerol-phosphate dehydratase. Similar to many e.g. HIS7_STRCO|P16247 from Streptomyces coelicolor (197 aa),FASTA results: opt: 763, E(): 0, (57.4% identity in 202 aa overlap). Belongs to the imidazoleglycerol-phosphate dehydratase family.; Probable imidazole glycerol-phosphate dehydratase HisB 1802667 hisB 886274 hisB Mycobacterium tuberculosis H37Rv Probable imidazole glycerol-phosphate dehydratase HisB NP_216117.1 1802035 D 83332 CDS NP_216118.1 15608740 885529 1802664..1803284 1 NC_000962.3 Rv1602, (MTCY336.02c), len: 206 aa. Probable hisH,amidotransferase. Similar to many e.g. HIS5_STRCO|P16249 from Streptomyces coelicolor (222 aa), FASTA results: opt: 872, E():0, (61.0% identity in 210 aa overlap). Contains glutamine amidotransferases class-I active site (PS00442). Belongs to the HisH family.; Probable amidotransferase HisH 1803284 hisH 885529 hisH Mycobacterium tuberculosis H37Rv Probable amidotransferase HisH NP_216118.1 1802664 D 83332 CDS NP_216119.1 15608741 885873 1803294..1804031 1 NC_000962.3 Rv1603, (MTV046.01-MTCY336.01c), len: 245 aa. Probable hisA, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, similar to many e.g. HIS4_STRCO|P16250 phosphoribosylformimino-5-aminoimidaz from Streptomyces coelicolor (240 aa), FASTA scores: opt: 1081, E(): 0, (69.0% identity in 239 aa overlap).; Probable phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase HisA 1804031 hisA 885873 hisA Mycobacterium tuberculosis H37Rv Probable phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase HisA NP_216119.1 1803294 D 83332 CDS NP_216120.1 15608742 885567 1804039..1804851 1 NC_000962.3 Rv1604, (MTV046.02), len: 270 aa. Probable impA,inositol monophosphatase, similar to many e.g. AF0059|AF005905_2 inositol monophosphate phosphatase from Mycobacterium smegmatis (276 aa), FASTA scores: opt: 1241,E(): 0, (70.5% identity in 261 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv3137 and Rv2701c.; Probable inositol-monophosphatase ImpA (imp) 1804851 impA 885567 impA Mycobacterium tuberculosis H37Rv Probable inositol-monophosphatase ImpA (imp) NP_216120.1 1804039 D 83332 CDS NP_216121.1 15608743 885261 1804853..1805656 1 NC_000962.3 Rv1605, (MTV046.03), len: 267 aa. Probable hisF,cyclase involved in histidine biosynthetic pathway, similar to many e.g. AF0304|AF030405_1 Corynebacterium glutamicum cyclase (257 aa), FASTA scores: opt: 1201, E(): 0, (71.9% identity in 256 aa overlap). Belongs to the HisA/HisF family.; Probable cyclase HisF 1805656 hisF 885261 hisF Mycobacterium tuberculosis H37Rv Probable cyclase HisF NP_216121.1 1804853 D 83332 CDS NP_216638.2 57116892 886011 1805653..1806000 1 NC_000962.3 Rv1606, (MTV046.04), len: 115 aa. Probable hisI,phosphoribosyl-AMP 1,6 cyclohydrolase, similar to several e.g. X82010|RSHISI_2 HISI from Rhodobacter sphaeroides (119 aa), FASTA scores: opt: 378, E(): 2.8e-21, (52.3% identity in 109 aa overlap); etc.; Probable phosphoribosyl-AMP 1,6 cyclohydrolase HisI 1806000 hisI 886011 hisI Mycobacterium tuberculosis H37Rv Probable phosphoribosyl-AMP 1,6 cyclohydrolase HisI NP_216638.2 1805653 D 83332 CDS NP_216123.1 15608745 885524 1806181..1807263 1 NC_000962.3 Rv1607, (MTV046.05), len: 360 aa. Probable chaA,ionic transporter integral membrane protein, putative calcium/proton antiporter, similar to many e.g. P31801|CHAA_ECOLI calcium/proton antiporter from Escherichia coli (366 aa), FASTA scores: opt: 736, E(): 0,(35.9% identity in 351 aa overlap). Equivalent to Mycobacterium leprae AL049913|MLCB1610_21 (77.7% identity in 364 aa overlap). Seems to belong to the CaCA family.; Probable ionic transporter integral membrane protein ChaA 1807263 chaA 885524 chaA Mycobacterium tuberculosis H37Rv Probable ionic transporter integral membrane protein ChaA NP_216123.1 1806181 D 83332 CDS NP_216124.1 15608746 885530 complement(1807298..1807762) 1 NC_000962.3 Rv1608c, (MTV046.06), len: 154 aa. Probable bcpB,peroxidoxin or bacterioferritin comigratory protein,similar to many, e.g. AE0003|ECAE000335_4 bacterioferritin comigratory protein from Escherichia coli K-12 MG1655 (156 aa), FASTA scores: opt: 329, E(): 1.2e-16, (38.2% identity in 152 aa overlap); Z97179|MLCL383_22 Mycobacterium leprae cosmid L383 (161 aa) (40.2% identity in 132 aa overlap). Also similar to Rv2428 AhpC, alkyl hydroperoxide reductase from Mycobacterium tuberculosis; and other Mycobacterium tuberculosis putative peroxidoxins Rv2521, Rv2238c,Rv1932.; Probable peroxidoxin BcpB 1807762 bcpB 885530 bcpB Mycobacterium tuberculosis H37Rv Probable peroxidoxin BcpB NP_216124.1 1807298 R 83332 CDS NP_216125.1 15608747 885040 1807903..1809453 1 NC_000962.3 Rv1609, (MTCY01B2.01, MTV046.07), len: 516 aa. trpE,anthranilate synthase component I. FASTA best: TRPE_CLOTM|P14953 anthranilate synthase component I from Clostridium thermocellum (494 aa), E(): 0, (42.6% identity in 498 aa overlap). Some similarity to Rv2386c|MTCY253.35,E(): 6.3e-17; and Rv3215|MTCY07D11.11c, E(): 5.7e-15. Belongs to the anthranilate synthase component I family.; Anthranilate synthase component I TrpE (glutamine amidotransferase) 1809453 trpE 885040 trpE Mycobacterium tuberculosis H37Rv Anthranilate synthase component I TrpE (glutamine amidotransferase) NP_216125.1 1807903 D 83332 CDS NP_216126.1 15608748 885293 1809443..1810150 1 NC_000962.3 Rv1610, (MTCY01B2.02), len: 235 aa. Possible conserved membrane protein. Equivalent to AL049913|MLCB1610_23 hypothetical protein from Mycobacterium leprae (264 aa), FASTA score: (65.8% identity in 231 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved membrane protein 1810150 885293 Rv1610 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_216126.1 1809443 D 83332 CDS NP_216127.1 15608749 885294 1810240..1811058 1 NC_000962.3 Rv1611, (MTCY01B2.03), len: 272 aa. Probable trpC,indole-3-glycerol phosphate synthase. Similar to Q55508|SLR0546 hypothetical 33.0 kDa protein from synechocystis SP (295 aa), FASTA score: opt: 26, E(): 7.6e-32, (44.2% identity in 265 aa overlap); also similar to TRPC_AZOBR|P26938 ndole-3-glycerol-phosphate synthaseindole-3-glycerol-phosphate synthase from Azospirillum brasilense (262 aa), FASTA score: opt: 596,E(): 4.8e-30, (43.8% identity in 258 aa overlap). Equivalent to AL0499 13|MLCB1610_24 from Mycobacterium leprae (272 aa) (90.8% identity in 272 aa overlap). Contains indole-3-glycerol phosphate synthase signature (PS00614). Belongs to the TrpC family.; Probable indole-3-glycerol phosphate synthase TrpC 1811058 trpC 885294 trpC Mycobacterium tuberculosis H37Rv Probable indole-3-glycerol phosphate synthase TrpC NP_216127.1 1810240 D 83332 CDS NP_216128.1 15608750 885297 1811127..1812359 1 NC_000962.3 Rv1612, (MTCY01B2.04), len: 410 aa. TrpB, tryptophan synthase beta chain. Equivalent to AL049913|MLCB1610_25 from Mycobacterium leprae (340 aa) (88.5% identity in 331 aa overlap). Similar to others e.g. TRPB_CAUCR|P12290 tryptophan synthase beta chain from Caulobacter crescentus (406 aa), FASTA scores: opt: 1662, E(): 0, (60.6% identity in 404 aa overlap). Belongs to the TrpB family. Tetramer of two alpha and two beta chains.; Tryptophan synthase, beta subunit TrpB 1812359 trpB 885297 trpB Mycobacterium tuberculosis H37Rv Tryptophan synthase, beta subunit TrpB NP_216128.1 1811127 D 83332 CDS NP_216129.1 15608751 885291 1812359..1813171 1 NC_000962.3 Rv1613, (MTCY01B2.05), len: 270 aa. Probable trpA,tryptophan synthase alpha chain. FASTA best: O68906|TRPA_MYCIT tryptophan synthase alpha chain from Mycobacterium intracellulare (271 aa), opt: 1442, E(): 0,(85.3% identity in 265 aa overlap).; Probable tryptophan synthase, alpha subunit TrpA 1813171 trpA 885291 trpA Mycobacterium tuberculosis H37Rv Probable tryptophan synthase, alpha subunit TrpA NP_216129.1 1812359 D 83332 CDS NP_216130.1 15608752 885292 1813171..1814577 1 NC_000962.3 Rv1614, (MTCY01B2.06), len: 468 aa. Possible lgt,prolipoprotein diacylglyceryl transferases, similar to many prolipoprotein diacylglyceryl transferases. FASTA scores: LGT_STAAU|P52282 prolipoprotein diacylglyceryl transferase from Staphylococcus aureus subsp. (279 aa), opt: 289,E():3.6e- 09, (31.5% identity in 257 aa overlap); AL096884|SC4G6_3 cosmid 4G6 from Streptomyces coelicolor (343 aa), opt: 735, E(): 4e-32, (46.5% identity in 391 aa overlap).; Possible prolipoprotein diacylglyceryl transferases Lgt 1814577 lgt 885292 lgt Mycobacterium tuberculosis H37Rv Possible prolipoprotein diacylglyceryl transferases Lgt NP_216130.1 1813171 D 83332 CDS NP_216131.1 15608753 885499 1815253..1815693 1 NC_000962.3 Rv1615, (MTCY01B2.07), len: 146 aa. Probable membrane protein; Probable membrane protein 1815693 885499 Rv1615 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216131.1 1815253 D 83332 CDS NP_216132.1 15608754 885498 1815683..1816081 1 NC_000962.3 Rv1616, (MTCY01B2.08), len: 132 aa. Conserved membrane protein, with some similarity to other hypothetical proteins e.g. AL096884|SC4G6_9 from Streptomyces coelicolor cosmid 4G6 (148 aa), FASTA scores: opt: 245, E(): 1.7e-1 0, (36.7% identity in 128 aa overlap); Q55401|SLL0543 hypothetical 16.5 kDa protein from synechocystis SP (148 aa), FASTA scores: opt: 225, E(): 6.5e-10, (35.9% identity in 117 aa overlap). Has cysteine cluster and contains a rubredoxin signature (PS00202).; hypothetical protein 1816081 885498 Rv1616 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216132.1 1815683 D 83332 CDS NP_216133.1 15608755 885501 1816189..1817607 1 NC_000962.3 Rv1617, (MTCY01B2.09), len: 472 aa. Probable pykA,pyruvate kinase. FASTA best: Q46078 pyruvate kinase from corynebacterium glutamicum (475 aa), opt: 2221, E(): 0,(72.2% identity in 468 aa overlap). Belongs to the pyruvate kinase family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010).; Probable pyruvate kinase PykA 1817607 pykA 885501 pykA Mycobacterium tuberculosis H37Rv Probable pyruvate kinase PykA NP_216133.1 1816189 D 83332 CDS NP_216134.1 15608756 885500 1817615..1818517 1 NC_000962.3 Rv1618, (MTCY01B2.10), len: 300 aa. Probable tesB1,acyl-CoA thioesterase II, similar to other acyl-CoA thioesterases e.g. TESB_ECOLI|P23911 acyl-CoA thioesterase II from Escherichia coli (285 aa), FASTA scores: opt: 495,E(): 2.9e-27, (32.5% identity in 283 aa overlap); etc. Also similar to Rv2605c|tesB2 from M. tuberculosis.; Probable acyl-CoA thioesterase II TesB1 1818517 tesB1 885500 tesB1 Mycobacterium tuberculosis H37Rv Probable acyl-CoA thioesterase II TesB1 NP_216134.1 1817615 D 83332 CDS NP_216135.1 15608757 885509 1818575..1820029 1 NC_000962.3 Rv1619, (MTCY01B2.11), len: 484 aa. Conserved membrane protein. Some similarity to N-terminus of P94974|Rv1640c|MTCY06H11.04c probable lysyl-tRNA synthetase 2 from Mycobacterium tuberculosis (1172 aa), FASTA scores: E(): 1.4e-16, (28.0% identity in 410 aa overlap); and similar in part to O69916| SC3C8.03C Putative intergral membrane protein from Streptomyces coelicolor cosmid 3C8 (589 aa), FASTA scores: opt: 453 E(): 8.4e-22, (31.3% identity in 313 aa overlap).; hypothetical protein 1820029 885509 Rv1619 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216135.1 1818575 D 83332 CDS NP_216136.1 15608758 885503 complement(1819963..1821693) 1 NC_000962.3 Rv1620c, (MTCY01B2.12c), len: 576 aa. Probable cydC,transmembrane ATP-binding protein ABC transporter involved in transport of component linked with the assembly of cytochrome (see citation below), similar to others e.g. CYDC_ECOLI|P23886 transport ATP-binding protein from Escherichia coli (573 aa), FASTA scores: opt: 631, E(): 1.6e-30, (28.5% identity in 569 aa overlap); C-terminal part of AL034355|SCD78_14 from Streptomyces coelicolor (1172 aa), FASTA scores: opt: 956, E(): 0, (38.8% identity in 554 aa overlap); etc. Contains (PS00211) ABC transporters family signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydC 1821693 cydC 885503 cydC Mycobacterium tuberculosis H37Rv Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydC NP_216136.1 1819963 R 83332 CDS NP_216137.1 15608759 885512 complement(1821690..1823273) 1 NC_000962.3 Rv1621c, (MTCY01B2.13c), len: 527 aa. Probable cydD,transmembrane ATP-binding protein ABC transporter involved in transport of component linked with the assembly of cytochrome (see citation below), similar to others e.g. P94366|CYDC_BACSU transport ATP-binding protein from Bacillus subtilis (567 aa), FASTA scores: opt: 784, E(): 0,(30.1% identity in 535 aa overlap); N-terminal part of AL034355|SCD78_14 from Streptomyces coelicolor (1172 aa),FASTA scores: opt: 1295, E(): 0, (44.6% identity in 534 aa overlap); etc. Also similar to Q11019|Y07D_MYCTU from Mycobacterium tuberculosis (579 aa), FASTA scores: opt: 530, E(): 6.9e-25, (29.1% identity in 530 aa overlap). Contains (PS00211) ABC transporters family signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydD 1823273 cydD 885512 cydD Mycobacterium tuberculosis H37Rv Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydD NP_216137.1 1821690 R 83332 CDS NP_216138.1 15608760 885510 complement(1823360..1824400) 1 NC_000962.3 Rv1622c, (MTCY01B2.14c), len: 346 aa. Probable cydB,cytochrome D ubiquinol oxidase subunit II, integral membrane protein, similar to others e.g. P11027|CYDB_ECOLI cytochrome D ubiquinol oxidase subunit II from Escherichia coli strain K12 (379 aa), FASTA scores: opt: 519, E(): 0,(32.3% identity in 372 aa overlap); P94365|CYDB_BACSU cytochrome D ubiquinol oxidase subunit II from Bacillus subtilis (338 aa), FASTA scores: opt: 824, E(): 0, (39.5% identity in 337 aa overlap); etc.; Probable integral membrane cytochrome D ubiquinol oxidase (subunit II) CydB (cytochrome BD-I oxidase subunit II) 1824400 cydB 885510 cydB Mycobacterium tuberculosis H37Rv Probable integral membrane cytochrome D ubiquinol oxidase (subunit II) CydB (cytochrome BD-I oxidase subunit II) NP_216138.1 1823360 R 83332 CDS YP_177824.1 57116893 885446 complement(1824430..1825887) 1 NC_000962.3 Rv1623c, (MTCY01B2.15c), len: 485 aa. Probable cydA (previously known as appC, but renamed cydA to conform with Mycobacterium smegmatis nomenclature), cytochrome D ubiquinol oxidase subunit I, integral membrane protein,similar to others e.g. P26459|APPC_ECOLI|CYXA|CBDA|B0978 cytochrome BD-II oxidase subunit I from Escherichia coli strain K12 (514 aa), FASTA scores: opt: 870, E(): 0, (35.9% identity in 485 aa overlap); AL034355|SCD78_12 from Streptomyces coelicolor (501 aa), FASTA scores: opt: 1099,E(): 0, (48.6% identity in 510 aa overlap); etc.; Probable integral membrane cytochrome D ubiquinol oxidase (subunit I) CydA (cytochrome BD-I oxidase subunit I) 1825887 cydA 885446 cydA Mycobacterium tuberculosis H37Rv Probable integral membrane cytochrome D ubiquinol oxidase (subunit I) CydA (cytochrome BD-I oxidase subunit I) YP_177824.1 1824430 R 83332 CDS NP_216140.1 15608762 885135 complement(1825998..1826585) 1 NC_000962.3 Rv1624c, (MTCY01B2.16c), len: 195 aa. Probable membrane protein, first start taken. Some similarity to Rv3155 nuoK, NADH dehydrogenase chain K from M. tuberculosis. Also similar to AAK72093.1|AF196488 hypothetical protein from Mycobacterium smegmatis (205 aa). Identities = 117/195 (60%).; Probable conserved membrane protein 1826585 885135 Rv1624c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_216140.1 1825998 R 83332 CDS NP_216141.2 57116894 888538 complement(1826614..1827945) 1 NC_000962.3 Rv1625c, (MT1661, MTCY01B2.17c), len: 443 aa. Cya,membrane-anchored adenylyl cyclase (see citations below). C-terminal half is similar to region in numerous eukaryotic adenylate and guanylate cyclases. N-terminal half hydrophobic. FASTA score: CYG2_RAT|P22717 guanylate cyclase soluble, beta-2 chain (682 aa), FASTA scores: opt: 552,E(): 2.7e-26, (40.3% identity in 226 aa overlap). Some similarity to Rv2435c|MTCY428.11 from Mycobacterium tuberculosis (730 aa), E(): 7e-19. Start changed since first submission (+25 aa). Belongs to adenylyl cyclase class-4/guanylyl cyclase family.; Membrane-anchored adenylyl cyclase Cya (ATP pyrophosphate-lyase) (adenylate cyclase) 1827945 cya 888538 cya Mycobacterium tuberculosis H37Rv Membrane-anchored adenylyl cyclase Cya (ATP pyrophosphate-lyase) (adenylate cyclase) NP_216141.2 1826614 R 83332 CDS NP_216142.1 15608764 885080 1828180..1828797 1 NC_000962.3 Rv1626, (MTCY01B2.18), len: 205 aa. Probable two-component response system transcriptional regulator,similar to many e.g. CHEY_BACSU|P24072 chemotaxis protein chey homolog (119 aa), FASTA scores: opt: 283, E(): 1.6e-16, (43.0% identity in 114 aa overlap). Also similar to AL109732|SC7H2_27 hypothetical protein from Streptomyces coelicolor (218 aa), opt: 880, E(): 0, (69.4% identity in 196 aa overlap).; Probable two-component system transcriptional regulator 1828797 885080 Rv1626 Mycobacterium tuberculosis H37Rv Probable two-component system transcriptional regulator NP_216142.1 1828180 D 83332 CDS NP_216143.1 15608765 885064 complement(1828865..1830073) 1 NC_000962.3 Rv1627c, (MTCY01B2.19c), len: 402 aa. Probable nonspecific lipid-transfer protein, similar to many lipid carrier proteins e.g. Q51797 acetyl CoA synthase from Pyrococcus furiosus (388 aa), FASTA scores: opt: 400, E(): 3.2e-18, (34.4% identity in 407 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis proteins Rv3523,Rv3540c, Rv0244, Rv2790c, Rv1323, etc.; Probable nonspecific lipid-transfer protein 1830073 885064 Rv1627c Mycobacterium tuberculosis H37Rv Probable nonspecific lipid-transfer protein NP_216143.1 1828865 R 83332 CDS NP_216144.1 15608766 885289 complement(1830070..1830561) 1 NC_000962.3 Rv1628c, (MTCY01B2.20c), len: 163 aa. Conserved protein, some similarity to others e.g. Q51796 ACAC protein in Pyrococcus furiosus (136 aa), FASTA scores: opt: 199,E(): 4.6e-06, (34.7% identity in 121 aa overlap).; hypothetical protein 1830561 885289 Rv1628c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216144.1 1830070 R 83332 CDS NP_216145.1 15608767 885074 1830665..1833379 1 NC_000962.3 Rv1629, (MTCY01B2.21), len: 904 aa. Probable polA,DNA polymerase I (see citations below). Has DNA polymerase family a signature (PS00447) at C-terminal end. FASTA best: DPO1_MYCTU|Q07700 DNA polymerase I from Mycobacterium tuberculosis (904 aa). Some similarity to Rv2090|MTCY49.30 (393 aa), E(): 2.2e-18, (38.7% identity in 292 aa overlap). Belongs to DNA polymerase type-a family.; Probable DNA polymerase I PolA 1833379 polA 885074 polA Mycobacterium tuberculosis H37Rv Probable DNA polymerase I PolA NP_216145.1 1830665 D 83332 CDS NP_216146.1 15608768 885188 1833542..1834987 1 NC_000962.3 Rv1630, (MTCY01B2.22), len: 481 aa. rpsA, 30S ribosomal protein S1. FASTA best: RS1_MYCLE|P46836 30s ribosomal protein S1 from Mycobacterium leprae (482 aa),opt: 2655, E(): 0, (87.2% identity in 483 aa overlap).; 30S ribosomal protein S1 RpsA 1834987 rpsA 885188 rpsA Mycobacterium tuberculosis H37Rv 30S ribosomal protein S1 RpsA NP_216146.1 1833542 D 83332 CDS NP_216147.1 15608769 885165 1835013..1836236 1 NC_000962.3 Rv1631, (MTCY01B2.23), len: 407 aa. Probable coaE,dephospho-CoA kinase, similar to many e.g. Q50178|ML1383|COAE_MYCLE dephospho-CoA kinase from Mycobacterium leprae (410 aa), FASTA scores: E(): 0, (77.5% identity in 409 aa overlap). Has ATP/GTP-binding site motif A (P-loop, PS00017) at N-terminus. In the N-terminal section; belongs to the CoaE family. In the C-terminal section; belongs to the UPF0157 (GrpB) family.; Probable dephospho-CoA kinase CoaE (dephosphocoenzyme a kinase) 1836236 coaE 885165 coaE Mycobacterium tuberculosis H37Rv Probable dephospho-CoA kinase CoaE (dephosphocoenzyme a kinase) NP_216147.1 1835013 D 83332 CDS NP_216148.1 15608770 885649 complement(1836387..1836830) 1 NC_000962.3 Rv1632c, (MTCY01B2.24c), len: 147 aa. Hypothetical unknown protein.; Hypothetical protein 1836830 885649 Rv1632c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216148.1 1836387 R 83332 CDS NP_216149.3 448824760 885249 1837075..1839171 1 NC_000962.3 Rv1633, (MTCY01B2.25), len: 698 aa. Probable uvrB,excinuclease ABC, subunit B; helicase (see Mizrahi & Andersen 1998; Sancar 1994); has ATP/GTP-binding site motif A (P-loop; PS00017) near N-terminus (see citation below). FASTA best: UVRB_MICLU|P10125 from Micrococcus luteus (709 aa), opt: 3268, E(): 0, (71.3% identity in 704 aa overlap). Also similar to Mycobacterium tuberculosis Rv2973c (recG); and Rv1020 (mfd). Belongs to the UVRB family.; Probable excinuclease ABC (subunit B-helicase) UvrB 1839171 uvrB 885249 uvrB Mycobacterium tuberculosis H37Rv Probable excinuclease ABC (subunit B-helicase) UvrB NP_216149.3 1837075 D 83332 CDS NP_216150.1 15608772 885115 1839168..1840583 1 NC_000962.3 Rv1634, (MTCY01B2.26), len: 471 aa. Possible drug efflux membrane protein of major facilitator superfamily (MFS), similar to many antibiotic resistance (efflux) proteins. FASTA best: Q56175 TU22 dTDP-glucose dehydrtatase (GRAE) from Streptomyces violaceoruber (557 aa), opt: 415,E(): 1.7e-17, (26.7% identity in 446 aa overlap). Relatives in Mycobacterium tuberculosis: MTCY369.27c, E(): 4.8e-12; MTCY20B11.14c, E(): 2.9e-10.; Possible drug efflux membrane protein 1840583 885115 Rv1634 Mycobacterium tuberculosis H37Rv Possible drug efflux membrane protein NP_216150.1 1839168 D 83332 CDS NP_216151.1 15608773 885100 complement(1840572..1842242) 1 NC_000962.3 Rv1635c, (MTCY01B2.27c), len: 556 aa. Probable mannosyltransferase (See Dinadayala et al., 2006). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent to CAC31770.1|AL583921 Mycobacterium leprae membrane protein (527 aa), Identities = 332/527 (62%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable mannosyltransferase Probable conserved transmembrane protein 1842242 885100 Rv1635c Mycobacterium tuberculosis H37Rv Probable mannosyltransferase Probable conserved transmembrane protein NP_216151.1 1840572 R 83332 CDS NP_216152.1 15608774 885473 1842451..1842891 1 NC_000962.3 Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3,iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188,E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025,(30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins (but lacks C-terminal region) including Rv2005c, Rv2623, Rv2026c,Rv1996, etc.; Iron-regulated universal stress protein family protein TB15.3 1842891 TB15.3 885473 TB15.3 Mycobacterium tuberculosis H37Rv Iron-regulated universal stress protein family protein TB15.3 NP_216152.1 1842451 D 83332 CDS NP_216153.1 15608775 885132 complement(1842898..1843692) 1 NC_000962.3 Rv1637c, (MTCY01B2.29c,MTCY06H11.01c), len: 264 aa. Conserved protein, some similarity to others e.g. P05446|GLO2_RHOBL probable hydroxyacylglutathione hydrolase (255 aa), FASTA scores: opt: 252, E(): 2e-09, (39.0% identity in 146 aa overlap). Also similar to Q9Z505|AL035591|SCC54.20 putative hydrolase from Streptomyces coelicolor (218 aa), FASTA scores: opt: 732,E(): 0, (52.3% identity in 220 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins and putative glyoxylases e.g. Rv0634c, Rv3677c, Rv2581c,Rv2260.; hypothetical protein 1843692 885132 Rv1637c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216153.1 1842898 R 83332 CDS NP_216154.1 15608776 885685 1843741..1846659 1 NC_000962.3 Rv1638, (MTCY06H11.01,MTCY06H11.02c), len: 972 aa. Probable uvrA, excinuclease ABC, subunit A; DNA-binding ATPase (see citations below), similar to many e.g. UVRA_ECOLI|P07671 excinuclease abc subunit A from Escherichia coli (940 aa), FASTA scores: opt: 2573, E(): 0,(56.2% identity in 951 aa overlap). Contains 2x PS00017 ATP/GTP-binding site motif A, PS00211 ABC transporters family signature, PS00211 ABC transporters family signature. Consists of three subunits; UVRA, UVRB and UVRC. Belongs to the ABC transporter family. UVRA subfamily.; Probable excinuclease ABC (subunit A-DNA-binding ATPase) UvrA 1846659 uvrA 885685 uvrA Mycobacterium tuberculosis H37Rv Probable excinuclease ABC (subunit A-DNA-binding ATPase) UvrA NP_216154.1 1843741 D 83332 CDS YP_177650.1 57116895 3205103 complement(1846716..1846973) 1 NC_000962.3 Rv1638A, len: 85 aa. Conserved hypothetical protein,similar to C-terminal part of P31511|35KD_MYCTU 35kd immunogenic protein from Mycobacterium tuberculosis (270 aa), FASTA scores: opt: 159, E(): 0.002, (50.90% identity in 55 aa overlap); and to Mycobacterium leprae ML0981 possible pseudogene, an orthologue of 35kd immunogenic protein from Mycobacterium tuberculosis. Size difference suggests possible gene fragment.; hypothetical protein 1846973 3205103 Rv1638A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177650.1 1846716 R 83332 CDS NP_216155.1 15608777 885642 complement(1846989..1848458) 1 NC_000962.3 Rv1639c, (MTCY06H11.03c), len: 489 aa. Conserved hypothetical membrane protein. Some similarity to P35866|YLI2_CORGL Hypothetical 45.7 kDa protein from Corynebacterium glutamicum (426 aa), FASTA scores: opt: 511, E(): 2.4e-23, (28.9% identity in 370 aa overlap). Contains PS00904 protein phenyltransferases alpha subunit repeat signature; hypothetical protein 1848458 885642 Rv1639c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216155.1 1846989 R 83332 CDS NP_216156.1 15608778 885428 complement(1848517..1852035) 1 NC_000962.3 Rv1640c, (MTCY06H11.04c), len: 1172 aa. lysX,lysyl-tRNA synthetase 2, probable two domain protein. N-terminal part (bases 1850153 to 1852033) is similar to AL023861|SC3C8_3 hypothetical membrane protein from Streptomyces coelicolor (589 aa), Fasta scores: opt: 1426,E(): 0, (44.6% identity in 585 aa overlap). The C-terminal part is similar to SYK_CRILO|P37879 lysyl-tRNA synthetases from Cricetulus longicaudatus (Long-tailed hamster) (597 aa), Fasta scores, opt: 985, E(): 0, (36.8% identity in 524 aa overlap). Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1, PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. This may indicate a frame shift but sequence has been checked and no error found. Belongs to class-II aminoacyl-tRNA synthetase family.; Lysyl-tRNA synthetase 2 LysX 1852035 lysX 885428 lysX Mycobacterium tuberculosis H37Rv Lysyl-tRNA synthetase 2 LysX NP_216156.1 1848517 R 83332 CDS NP_216157.1 15608779 885478 1852273..1852878 1 NC_000962.3 Rv1641, (MTCY06H11.05), len: 201 aa. Probable infC,initiation factor if-3, similar to many e.g. IF3_BACST|P03000 initiation factor if-3 from Bacillus stearothermophilus (171 aa), FASTA scores: opt: 560, E(): 1.9e-27, (50.6% identity in 166 aa overlap). Note that an AUC initiation codon has been used, the Bacillus (IF3_BACSU) and Escherichia coli (IF3_ECOLI) proteins use an AUU initiation codon, and the Myxococcus xanthus (DSG_MYXXA) homolog uses a AUC. Belongs to the if-3 family.; Probable initiation factor if-3 InfC 1852878 infC 885478 infC Mycobacterium tuberculosis H37Rv Probable initiation factor if-3 InfC NP_216157.1 1852273 D 83332 CDS NP_216158.1 15608780 885145 1852928..1853122 1 NC_000962.3 Rv1642, (MTCY06H11.06), len: 64 aa. rpmI, 50S ribosomal protein L35, similar to several e.g. RL35_SYNY3|P48959 from Synechocystis sp. (67 aa), fasta scores: opt: 179, E(): 2.7e-08, (51.6% identity in 64 aa overlap). Belongs to the L35P family of ribosomal proteins.; 50S ribosomal protein L35 RpmI 1853122 rpmI 885145 rpmI Mycobacterium tuberculosis H37Rv 50S ribosomal protein L35 RpmI NP_216158.1 1852928 D 83332 CDS NP_216159.1 15608781 885455 1853184..1853573 1 NC_000962.3 Rv1643, (MTCY06H11.07), len: 129 aa. rplT, 50S ribosomal protein L20, similar to several e.g. RL20_ECOLI|P02421 from Escherichia coli (117 aa), FASTA scores: opt: 438, E(): 5.8e-24, (60.3% identity in 116 aa overlap). Contains PS00937 Ribosomal protein L20 signature.; 50S ribosomal protein L20 RplT 1853573 rplT 885455 rplT Mycobacterium tuberculosis H37Rv 50S ribosomal protein L20 RplT NP_216159.1 1853184 D 83332 CDS NP_216160.1 15608782 885163 1853606..1854388 1 NC_000962.3 Rv1644, (MTCY06H11.08), len: 260 aa. Possible tsnR,23S rRNA methyltransferase, similar to several e.g. TSNR_STRLU|P52393 from Streptomyces laurentii (270 aa),FASTA scores: opt: 276, E(): 3.6e-11, (27.6% identity in 261 aa overlap). Also similar to M. tuberculosis hypothetical proteins Rv0881, Rv3579c, and Rv0380c.; Possible 23S rRNA methyltransferase TsnR 1854388 tsnR 885163 tsnR Mycobacterium tuberculosis H37Rv Possible 23S rRNA methyltransferase TsnR NP_216160.1 1853606 D 83332 CDS NP_216161.1 15608783 885287 complement(1854399..1855454) 1 NC_000962.3 Rv1645c, (MTCY06H11.10c), len: 351 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O53837|Rv0826|MTV043.18 (351 aa), FASTA scores: (57.5% identity in 299 aa overlap); Q10519|Rv2237|YM37_MYCTU (255 aa), O53682|Rv0276 (306 aa).; hypothetical protein 1855454 885287 Rv1645c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216161.1 1854399 R 83332 CDS YP_177825.1 57116896 885486 1855764..1856696 1 NC_000962.3 Rv1646, (MTCY06H11.11), len: 310 aa. PE17, Member of the Mycobacterium tuberculosis PE family of proteins (see citation below), similar to many e.g. YW36_MYCTU|Q10873 hypothetical 53.7 kd protein cy39.36c (558 aa), FASTA scores, opt: 411, E(): 1.3e-15, (34.4% identity in 320 aa overlap).; PE family protein PE17 1856696 PE17 885486 PE17 Mycobacterium tuberculosis H37Rv PE family protein PE17 YP_177825.1 1855764 D 83332 CDS NP_216163.1 15608785 885432 1856774..1857724 1 NC_000962.3 Rv1647, (MTCY06H11.12), len: 316 aa. Adenylate cyclase, some similarity to other Mycobacterium tuberculosis proteins e.g. Q11055|Rv1264|YC64_MYCTU 42.2 kDa protein (397 aa), FASTA scores: opt: 197, E(): 9.4e-06,(27.1% identity in 181 aa overlap) and Q10400|Rv2212|YM12_MYCTU (378 aa). Belongs to adenylyl cyclase class-3 family.; Adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) 1857724 885432 Rv1647 Mycobacterium tuberculosis H37Rv Adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) NP_216163.1 1856774 D 83332 CDS NP_216164.1 15608786 885065 1857731..1858537 1 NC_000962.3 Rv1648, (MTCY06H11.13), len: 268 aa. Probable transmembrane protein, some similarity to Rv3434c|MTCY77.06C (237 aa), FASTA scores: E(): 0.00039,(31.4% identity in 194 aa overlap).; Probable transmembrane protein 1858537 885065 Rv1648 Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_216164.1 1857731 D 83332 CDS NP_216165.3 448824761 885105 1858733..1859758 1 NC_000962.3 Rv1649, (MTCY06H11.14), len: 341 aa. Probable pheS,Phenylalanyl-tRNA synthetase alpha chain, similar to several e.g. SYFA_ECOLI|P08312 from Escherichia coli (327 aa), FASTA scores: opt: 978, E(): 0, (46.5% identity in 331 aa overlap). Homology suggests this start site, but there is a potential rbs upstream of a gtg 30 bp upstream; contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. PHE-tRNA synthetase alpha chain subfamily 1.; Probable phenylalanyl-tRNA synthetase, alpha chain PheS 1859758 pheS 885105 pheS Mycobacterium tuberculosis H37Rv Probable phenylalanyl-tRNA synthetase, alpha chain PheS NP_216165.3 1858733 D 83332 CDS NP_216166.1 15608788 885283 1859758..1862253 1 NC_000962.3 Rv1650, (MTCY06H11.15), len: 831 aa. Probable pheT,Phenylalanyl-tRNA synthetase beta chain, similar to several e.g. SYFB_ECOLI|P07395 from Escherichia coli (795 aa),FASTA scores: opt: 995, E(): 0, (31.8% identity in 847 aa overlap). Belongs to the phenylalanyl-tRNA synthetase beta chain family - subfamily 1.; Probable phenylalanyl-tRNA synthetase, beta chain PheT 1862253 pheT 885283 pheT Mycobacterium tuberculosis H37Rv Probable phenylalanyl-tRNA synthetase, beta chain PheT NP_216166.1 1859758 D 83332 CDS YP_177826.1 57116897 885174 complement(1862347..1865382) 1 NC_000962.3 Rv1651c, (MTCY06H11.16c), len: 1011 aa. PE_PGRS30,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citations below),similar to many e.g. Q10637|Y03A_MYCTU hypothetical glycine-rich 49.6 kd protein (603 aa), FASTA scores: opt: 1757, E(): 0, (50.8% identity in 714aa overlap). The transcription of this CDS seems to be activated in macrophages (see Ramakrishnan et al., 2000).; PE-PGRS family protein PE_PGRS30 1865382 PE_PGRS30 885174 PE_PGRS30 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS30 YP_177826.1 1862347 R 83332 CDS NP_216168.1 15608790 885278 1865576..1866634 1 NC_000962.3 Rv1652, (MTCY06H11.17), len: 352 aa. Probable argC,N-acetyl-gamma-glutamyl-phosphate reductase, similar to many e.g. ARGC_STRCL|P54896 from Streptomyces clavuligerus (340 aa), FASTA scores: opt: 1119, E(): 0, (56.9% identity in 350 aa overlap); etc. Belongs to the NAGSA dehydrogenase family.; Probable N-acetyl-gamma-glutamyl-phoshate reductase ArgC 1866634 argC 885278 argC Mycobacterium tuberculosis H37Rv Probable N-acetyl-gamma-glutamyl-phoshate reductase ArgC NP_216168.1 1865576 D 83332 CDS NP_216169.1 15608791 885125 1866631..1867845 1 NC_000962.3 Rv1653, (MTCY06H11.18), len: 404 aa. Probable argJ,Glutamate n-acetyltransferase, similar to ARGJ_BACSU|P36843 from Bacillus subtilis (406 aa), fasta scores: opt: 727,E(): 0, (36.3% identity in 410 a a overlap).; Probable glutamate N-acetyltransferase ArgJ 1867845 argJ 885125 argJ Mycobacterium tuberculosis H37Rv Probable glutamate N-acetyltransferase ArgJ NP_216169.1 1866631 D 83332 CDS NP_216170.1 15608792 888076 1867842..1868726 1 NC_000962.3 Rv1654, (MTCY06H11.19), len: 294 aa. Probable argB,Acetylglutamate kinase, similar to ARGB_CORGL|Q59281 (294 aa), FASTA scores: opt: 1209, E(): 0, (64.4% identity in 270 aa overlap). Belongs to the acetylglutamate kinase family.; Probable acetylglutamate kinase ArgB 1868726 argB 888076 argB Mycobacterium tuberculosis H37Rv Probable acetylglutamate kinase ArgB NP_216170.1 1867842 D 83332 CDS NP_216171.1 15608793 885187 1868723..1869925 1 NC_000962.3 Rv1655, (MTCY06H11.20), len: 400 aa. Probable argD,Acetylornithine aminotransferase, similar to ARGD_ECOLI|P18335 (406 aa), FASTA scores: opt: 958, E(): 0,(38.6% identity in 404 aa overlap), contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases.; Probable acetylornithine aminotransferase ArgD 1869925 argD 885187 argD Mycobacterium tuberculosis H37Rv Probable acetylornithine aminotransferase ArgD NP_216171.1 1868723 D 83332 CDS NP_216172.1 15608794 885462 1869922..1870845 1 NC_000962.3 Rv1656, (MTCY06H11.21), len: 307 aa. Probable argF,ornithine carbamoyltransferase, anabolic (see citation below), almost identical to OTCA_MYCBO|Q02095 ornithine carbamoyltransferase, anabolic from Mycobacterium bovis (307 aa), FASTA scores: opt: 1980, E(): 0, (99.0% identity in 307 aa overlap); contains PS00097 Aspartate and ornithine carbamoyltransferases signature. Belongs to the ATCases/OTCases family.; Probable ornithine carbamoyltransferase, anabolic ArgF 1870845 argF 885462 argF Mycobacterium tuberculosis H37Rv Probable ornithine carbamoyltransferase, anabolic ArgF NP_216172.1 1869922 D 83332 CDS NP_216173.1 15608795 885091 1870842..1871354 1 NC_000962.3 Rv1657, (MTCY06H11.22), len: 170 aa. Probable argR,Arginine repressor (alternate gene name: ahrC). Similar to AHRC_BACSU|P17893 arginine hydroximate resistance protein from Bacillus subtilis (149 aa), FASTA scores: opt: 283,E(): 1.8e-11, (34.5% identity in 142 aa overlap); and ARGR_ECOLI|P15282 arginine repressor from Escherichia coli (156 aa), FASTA scores: opt: 194, E(): 6.4e-06, (30.8% identity in 146 aa overlap). Belongs to the ArgR family.; Probable arginine repressor ArgR (AHRC) 1871354 argR 885091 argR Mycobacterium tuberculosis H37Rv Probable arginine repressor ArgR (AHRC) NP_216173.1 1870842 D 83332 CDS NP_216174.1 15608796 885645 1871363..1872559 1 NC_000962.3 Rv1658, (MTCY06H11.23), len: 398 aa. Probable argG,Argininosuccinate synthase, similar to ASSY_STRCL|P50986 argininosuccinate synthase from Streptomyces clavuligerus (397 aa), FASTA scores: opt: 1873, E(): 0, (67.8% identity in 397 aa overlap); contains PS00564 Argininosuccinate synthase signature 1, PS00565 Argininosuccinate synthase signature 2. Belongs to the argininosuccinate synthase family.; Probable argininosuccinate synthase ArgG 1872559 argG 885645 argG Mycobacterium tuberculosis H37Rv Probable argininosuccinate synthase ArgG NP_216174.1 1871363 D 83332 CDS NP_216175.1 15608797 885365 1872639..1874051 1 NC_000962.3 Rv1659, (MTCY06H11.24), len: 470 aa. Probable argH,Argininosuccinate lyase, similar to ARLY_ECOLI|P11447 argininosuccinate lyase from Escherichia coli (457 aa),FASTA scores: opt: 1091, E(): 0, (42.5% identity in 461 aa overlap); contains PS00017 ATP/GTP-binding site motif A,PS00163 Fumarate lyases signature. Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.; Probable argininosuccinate lyase ArgH 1874051 argH 885365 argH Mycobacterium tuberculosis H37Rv Probable argininosuccinate lyase ArgH NP_216175.1 1872639 D 83332 CDS NP_216176.1 15608798 885112 1874160..1875221 1 NC_000962.3 Rv1660, (MTCY06H11.25), len: 353 aa. pks10, chalcone synthase, similar to BCSA_BACSU|P54157 putative chalcone synthase from B. subtilis (365 aa), FASTA scores: opt: 701,E(): 0, (33.1% identity in 362 aa overlap). Also similar to M. tuberculosis Rv1665|pks11 polyketide synthase (chalcone synthase); and Rv1372|pks18 polyketide synthase. Other upstream initiation sites are possible but homology suggests this start. Note pks10 has been shown to be involved in the biosynthesis of phthiocerol.; Chalcone synthase Pks10 1875221 pks10 885112 pks10 Mycobacterium tuberculosis H37Rv Chalcone synthase Pks10 NP_216176.1 1874160 D 83332 CDS NP_216177.1 15608799 885108 1875304..1881684 1 NC_000962.3 Rv1661, (MTCY06H11.26), len: 2126 aa. Probable pks7,polyketide synthase, similar to many e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa), FASTA scores: E(): 0, (48.8% identity in 2131 aa overlap); also similar to Mycobacterium tuberculosis pks12. Contains PS00606 Beta-ketoacyl synthases active site, PS00012 Phosphopantetheine attachment site.; Probable polyketide synthase Pks7 1881684 pks7 885108 pks7 Mycobacterium tuberculosis H37Rv Probable polyketide synthase Pks7 NP_216177.1 1875304 D 83332 CDS NP_216178.1 15608800 885527 1881704..1886512 1 NC_000962.3 Rv1662, (MTCY275.01-MTCY06H11.27), len: 1602 aa. Probable pks8, polyketide synthase, similar to many polyketide synthases e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 3319, E(): 0, (45.8% identity in 1619 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks12. Contains PS00606 Beta-ketoacyl synthases active site and PS01162 Quinone oxidoreductase/zeta-crystallin signature. Note that the similarity extends into the downstream ORF Rv1663 (MTCY275.02), and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found.; Probable polyketide synthase Pks8 1886512 pks8 885527 pks8 Mycobacterium tuberculosis H37Rv Probable polyketide synthase Pks8 NP_216178.1 1881704 D 83332 CDS NP_216179.1 15608801 885523 1886512..1888020 1 NC_000962.3 Rv1663, (MTCY275.02), len: 502 aa. Probable pks17,polyketide synthase, similar to other polyketide synthases e g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa) from Saccharopolyspora erythraea (Streptomyces erythraeus), FASTA scores: opt: 1207, E(): 0,(43.9% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks1. Note that the similarity extends into the upstream ORF Rv1662 (MTCY275.01) and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found. Contains PS00012 Phosphopantetheine attachment site.; Probable polyketide synthase Pks17 1888020 pks17 885523 pks17 Mycobacterium tuberculosis H37Rv Probable polyketide synthase Pks17 NP_216179.1 1886512 D 83332 CDS NP_216180.1 15608802 885519 1888026..1891079 1 NC_000962.3 Rv1664, (MTCY275.03), len: 1017 aa. Probable pks9,polyketide synthase, similar to OL56_STRAT|Q07017 oleandomycin polyketide synthase, modules 5 and 6 from Streptomyces antibioticus (3519 aa), FASTA scores: opt: 1767, E(): 0, (41.6% identity in 919 aa overlap). Similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks6, pks8, etc. Contains PS00012 Phosphopantetheine attachment site.; Probable polyketide synthase Pks9 1891079 pks9 885519 pks9 Mycobacterium tuberculosis H37Rv Probable polyketide synthase Pks9 NP_216180.1 1888026 D 83332 CDS NP_216181.1 15608803 885525 1891226..1892287 1 NC_000962.3 Rv1665, (MTCY275.04-MTV047.01), len: 353 aa. pks11,chalcone synthase, some similarity to BCSA_BACSU|P54157 putative chalcone synthase from Bacillus subtilis (365 aa),FASTA scores: opt: 615, E(): 6.2e-32, (33.4% identity in 308 aa overlap); and to many plant chalcone synthases e.g. CHS_VIGUN|P51089 chalcone synthase (388 aa), FASTA scores: opt: 391, E(): 7.8e-18, (27.2% identity in 349 aa overlap). Highly similar to upstream ORF Rv1660|MTCY06H11.25 pks10 (72.7% identity in 308 aa overlap); and Rv1372 pks18.; Chalcone synthase Pks11 1892287 pks11 885525 pks11 Mycobacterium tuberculosis H37Rv Chalcone synthase Pks11 NP_216181.1 1891226 D 83332 CDS NP_216182.1 15608804 885528 complement(1892270..1893562) 1 NC_000962.3 Rv1666c, (MT1706, MTV047.02c), len: 430 aa. Probable cyp139, cytochrome P450, similar to many e.g. U38537|APU38537_7 from Anabaena sp. (459 aa), FASTA scores: opt: 516, E(): 1.7e-26, (25.8% identity in 418 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Probable cytochrome P450 139 Cyp139 1893562 cyp139 885528 cyp139 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 139 Cyp139 NP_216182.1 1892270 R 83332 CDS NP_216183.1 15608805 885518 complement(1893577..1894230) 1 NC_000962.3 Rv1667c, (MTV047.03c), len: 217 aa. Probable second part of macrolide-transport ATP-binding protein ABC transporter (see citation below), with similarity to C-terminal end of putative ABC transporters/ATP binding proteins, e.g. Z99108|BSUB0005_6 ABC transporter (ATP-binding protein) homolog yfmR from Bacillus subtilis (629 aa), FASTA scores: opt: 411, E(): 6.9e-17, (37.8% identity in 217 aa overlap); etc. Similarity to other NBD components of ABC transporters suggests that Rv1667c and Rv1668c should be contiguous. However, sequence has been checked and no errors found, also same sequence in M. tuberculosis CSU93 and Mycobacterium bovis.; Probable second part of macrolide-transport ATP-binding protein ABC transporter 1894230 885518 Rv1667c Mycobacterium tuberculosis H37Rv Probable second part of macrolide-transport ATP-binding protein ABC transporter NP_216183.1 1893577 R 83332 CDS NP_216184.1 15608806 885522 complement(1894224..1895342) 1 NC_000962.3 Rv1668c, (MTV047.04c), len: 372 aa. Probable first part of macrolide-transport ATP-binding protein ABC transporter (see citation below), similar to many ATP-binding proteins ABC transporter e.g. X80735|SEABCT_1|Q54072 Saccharopolyspora erythraea ertX gene (481 aa), FASTA scores: opt: 938, E(): 0, (45.6% identity in 353 aa overlap); etc. Similarity to other NBD components of ABC transporters suggests that Rv1667c and Rv1668c should be contiguous. However, sequence has been checked and no error found, also same sequence in Mycobacterium tuberculosis CSU93 and Mycobacterium bovis. Contains PS00211 ABC transporters family signature and two times PS00017 ATP/GTP-binding site motif A. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable first part of macrolide-transport ATP-binding protein ABC transporter 1895342 885522 Rv1668c Mycobacterium tuberculosis H37Rv Probable first part of macrolide-transport ATP-binding protein ABC transporter NP_216184.1 1894224 R 83332 CDS NP_216185.1 15608807 885701 1895725..1896087 1 NC_000962.3 Rv1669, (MTV047.04B), len: 120 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008).; Hypothetical protein 1896087 885701 Rv1669 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216185.1 1895725 D 83332 CDS NP_216186.1 15608808 885702 1896120..1896467 1 NC_000962.3 Rv1670, (MTV047.05), len: 115 aa. Conserved hypothetical protein, highly similar to D90908|D90908_87 Hypothetical protein of Synechocystis sp. PCC6803 complete (94 aa), FASTA scores opt: 378, E(): 3.5e-2, (55.2% identity in 96 aa overlap); also shows some similarity to Mycobacterium tuberculosis hypothetical proteins e.g. C-terminal region of O53404|Rv1056 (254 aa), and P96817|Rv0140 (126 aa).; hypothetical protein 1896467 885702 Rv1670 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216186.1 1896120 D 83332 CDS NP_216187.1 15608809 885698 1896475..1896867 1 NC_000962.3 Rv1671, (MTV047.06), len: 130 aa. Probable membrane protein. Weak similarity to mercuric transport proteins.; Probable membrane protein 1896867 885698 Rv1671 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216187.1 1896475 D 83332 CDS NP_216188.1 15608810 885700 complement(1896876..1898207) 1 NC_000962.3 Rv1672c, (MTV047.07c), len: 443 aa. Probable conserved integral membrane transport protein, major facilitator superfamily, similar to several phthalate transporters or tartrate transporters e.g. U25634|AVU25634_2 Agrobacterium vitis plasmid pTrAB (433 aa), FASTA scores: opt: 914, E(): 0, (37.1% identity in 426 aa overlap); etc.; Probable conserved integral membrane transport protein 1898207 885700 Rv1672c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein NP_216188.1 1896876 R 83332 CDS NP_216189.1 15608811 885695 complement(1898300..1899232) 1 NC_000962.3 Rv1673c, (MTV047.08c), len: 310 aa. Conserved hypothetical protein, shows weak similarity to P44103|YA48_HAEIN Hypothetical protein HI10 48 precursor (369 aa), FASTA scores: E(): 8.3e-11, (26.1% identity in 330 aa overlap).; hypothetical protein 1899232 885695 Rv1673c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216189.1 1898300 R 83332 CDS NP_216190.1 15608812 885696 complement(1899260..1899916) 1 NC_000962.3 Rv1674c, (MTV047.09c), len: 218 aa. Probable transcriptional regulatory protein. Highly similar to AJ005575|SPE005575_2 Streptomyces peucetius (226 aa), FASTA scores: opt: 662, E(): 0, (50.0% identity in 208 aa overlap). Similar to Rv0324|Z96800|MTCY63.29 M. tuberculosis cosmid (226 aa), FASTA scores: opt: 579, E(): 0, (45.3% identity in 214 aa overlap). N-terminus is similar to transcriptional activators e.g. MERR_STRLI|P30346 probable mercury resistance operon regulator (125 aa), FASTA scores: opt: 183, E(): 1.9e-06,(35.6% identity in 90 aa overlap). Contains PS00380 Rhodanese signature 1.; Probable transcriptional regulatory protein 1899916 885696 Rv1674c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_216190.1 1899260 R 83332 CDS NP_216191.1 15608813 885693 complement(1900241..1900975) 1 NC_000962.3 Rv1675c, (MTV047.10c), len: 244 aa. Probable cmr,cAMP and macrophage regulator, transcriptional regulatory protein, weak similarity to D00496|LBATRP_7 trp operon from Lactobacillus casei (219 aa), FASTA scores: opt: 172, E(): 0.00011, (26.9% identity in 186 aa overlap).; Probable transcriptional regulatory protein Cmr 1900975 cmr 885693 cmr Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein Cmr NP_216191.1 1900241 R 83332 CDS NP_216192.1 15608814 885694 1901047..1901751 1 NC_000962.3 Rv1676, (MTV047.11), len: 234 aa. Unknown protein.; hypothetical protein 1901751 885694 Rv1676 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216192.1 1901047 D 83332 CDS NP_216193.1 15608815 885690 1901748..1902296 1 NC_000962.3 Rv1677, (MTV047.12), len: 182 aa. Probable dsbF,conserved lipoprotein possibly involved in thiol:disulfide interchange. Highly similar to C-terminus of Z74024|MTCY274.09 mpt53 soluble secreted antigen precursor from Mycobacterium tuberculosis (173 aa), FASTA scores: opt: 482, E(): 3.6e-23, (52.8% identity in 142 aa overlap) . Also some similarity to P52237|TIPB_PSEFL thiol:disulfide interchange protein TIPB precursor from Pseudomonas fluorescens (178 aa), FASTA scores: opt: 190, E(): 4.4e-05,(28.5% identity in 151 aa overlap); and P33926|DSBE_ECOLI thiol:disulfide interchange protein from Escherichia coli (185 aa), FASTA scores: opt: 194, E(): 2.6e-05, (29.1% identity in 175 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS00194 Thioredoxin family active site. Nucleotide position 1901816 in the genome sequence has been corrected, A:G resulting in Q23Q.; Probable conserved lipoprotein DsbF 1902296 dsbF 885690 dsbF Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein DsbF NP_216193.1 1901748 D 83332 CDS NP_216194.1 15608816 885691 1902397..1903299 1 NC_000962.3 Rv1678, (MTV047.13), len: 300 aa. Probable integral membrane protein.; Probable integral membrane protein 1903299 885691 Rv1678 Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_216194.1 1902397 D 83332 CDS NP_216195.1 15608817 885688 1903299..1904420 1 NC_000962.3 Rv1679, (MTV047.14, MTCI125.01), len: 373 aa. Possible fadE16, acyl-CoA dehydrogenase, similar to acyl/butyryl-CoA dehydrogenases e.g. NP_244665.1|NC_002570 acyl-CoA dehydrogenase from Bacillus halodurans (380 aa); NP_000008.1|NM_000017 acyl-Coenzyme A dehydrogenase from Homo sapiens (412 aa); Z99113|BSUB0010_119 from Bacillus subtilis (380 aa), FASTA scores: opt: 439, E(): 3.4e-20,(29.6% identity in 287 aa overlap); etc. Weakly similar to many dehydrogenases and to P31571|CAIA_ECOLI probable carnitine operon oxidoreductase from Escherichia coli (380 aa), FASTA scores: opt: 109, E(): 0.0066, (28.6% identity in 98 aa overlap).; Possible acyl-CoA dehydrogenase FadE16 1904420 fadE16 885688 fadE16 Mycobacterium tuberculosis H37Rv Possible acyl-CoA dehydrogenase FadE16 NP_216195.1 1903299 D 83332 CDS NP_216196.1 15608818 885689 1904429..1905253 1 NC_000962.3 Rv1680, (MTCI125.02), len: 274 aa. Hypothetical unknown protein.; Hypothetical protein 1905253 885689 Rv1680 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216196.1 1904429 D 83332 CDS NP_216197.1 15608819 885683 1905250..1906242 1 NC_000962.3 Rv1681, (MTCI125.03), len: 330 aa. Possible moeX,Molybdopterin biosynthesis protein, has weak similarity to MOAA_ECOLI|P30745 molybdenum cofactor biosynthesis protein (329 aa), FASTA scores: opt: 162, E(): 0.00081, (27.7% identity in 224 aa overlap) and to Rv3109|MTCY164.19 MoaA from Mycobacterium tuberculosis (28.5% identity in 165 aa overlap).; Possible molybdopterin biosynthesis protein MoeX 1906242 moeX 885683 moeX Mycobacterium tuberculosis H37Rv Possible molybdopterin biosynthesis protein MoeX NP_216197.1 1905250 D 83332 CDS NP_216198.1 15608820 885686 1906403..1907320 1 NC_000962.3 Rv1682, (MTCI125.04), len: 305 aa. Probable coiled-coil structural protein, weakly similar to many paramyosins, kinesins and plectins e.g. MYSP_ONCVO|Q02171 paramyosin from onchocerca volvulus (879 aa), fasta scores: opt: 180, E():2.6e-08, (24.4% identity in 234 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical coiled-coil proteins (wag31 antigen 84) Rv2145c and Rv2927c.; Probable coiled-coil structural protein 1907320 885686 Rv1682 Mycobacterium tuberculosis H37Rv Probable coiled-coil structural protein NP_216198.1 1906403 D 83332 CDS NP_216199.1 15608821 885687 1907594..1910593 1 NC_000962.3 Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tuberculosis protein MTCI364.18 (27.4% identity in 583 aa overlap). Contains PS00120 Lipases,serine active site.; Possible bifunctional enzyme; long-chain acyl-CoA synthase and lipase 1910593 885687 Rv1683 Mycobacterium tuberculosis H37Rv Possible bifunctional enzyme; long-chain acyl-CoA synthase and lipase NP_216199.1 1907594 D 83332 CDS NP_216200.1 15608822 885680 1910586..1910810 1 NC_000962.3 Rv1684, (MTCI125.06), len: 74 aa. Conserved hypothetical protein, similar to P75844|YCAR_ECOLI Protein YCAR from Escherichia coli (60 aa), FASTA scores: opt: 108,E(): 0.00022, (39.0% identity in 59 aa overlap).; hypothetical protein 1910810 885680 Rv1684 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216200.1 1910586 D 83332 CDS NP_216201.1 15608823 885682 complement(1910776..1911399) 1 NC_000962.3 Rv1685c, (MTCI125.07c), len: 207 aa. Conserved hypothetical protein, some similarity to other Mycobacterium tuberculosis hypothetical regulatory proteins e.g. Q10774|Rv1556|YF56_MYCTU (202 aa), FASTA scores: opt: 111, E(): 1.7e-05, (24.1% identity in 195 aa overlap); and P95215|Rv0258c|MTCY06A4.02c (151 aa) FASTA scores: (32.9% identity in 140 aa overlap); also similar to Q9X8G9|SCE7.13C|AL049819 putative Streptomyces coelicolor transcriptional regulator (204 aa), FASTA scores: opt: 480,E(): 6.4e-25, (40.4% identity in 203 aa overlap).; hypothetical protein 1911399 885682 Rv1685c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216201.1 1910776 R 83332 CDS NP_216202.1 15608824 885672 complement(1911401..1912081) 1 NC_000962.3 Rv1686c, (MTCI125.08c), len: 226 aa. Probable conserved integral membrane protein ABC transporter (see citation below), similar to AL049819|SCE7.05 putative integral membrane protein from Streptomyces coelicolor (266 aa), FASTA sacores: opt: 661, E(): 0, (45.1% identity in 226 aa overlap); and Q53627|U43537 membrane protein involved in mithramycin resistance from streptomyces argillaceus (233 aa), FASTA scores: opt: 222, E(): 5.4e-10,(28.7% identity in 216 aa overlap).; Probable conserved integral membrane protein ABC transporter 1912081 885672 Rv1686c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein ABC transporter NP_216202.1 1911401 R 83332 CDS NP_216203.1 15608825 885678 complement(1912153..1912920) 1 NC_000962.3 Rv1687c, (MTCI125.09c), len: 255 aa. Probable conserved ATP-binding protein ABC transporter (see citation below), similar to many ABC-type transporters e.g. P55476|NODI_RHISN nodulation ATP-binding protein I from Rhizobium sp. (343 aa), FASTA scores: opt: 479, E(): 3.7e-23, (34.6% identity in 243 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC-type transporters e.g. MTCY19H9.04 (34.5% identity in 238 aa overlap). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). Also contains PS00039 dead-box subfamily ATP-dependent helicases signature, though this may be spurious.; Probable conserved ATP-binding protein ABC transporter 1912920 885678 Rv1687c Mycobacterium tuberculosis H37Rv Probable conserved ATP-binding protein ABC transporter NP_216203.1 1912153 R 83332 CDS NP_216204.1 15608826 885679 1912979..1913590 1 NC_000962.3 Rv1688, (MTCI125.10), len: 203 aa. Possible mpg,3-methyladenine DNA glycosylase (see citation below),similar to several eukaryotic 3-methylpurine DNA glycosylases and 3-methyladenine DNA glycosylases e.g. Q39147|X76169 3-methyladenine glycosylase from Arabidobsis thaliana (254 aa), FASTA scores: opt: 297, E(): 8.3e-15,(31.8% identity in 198 aa overlap) and P29372|3MG_HUMAN dna-3-methyladenine glycosidase (298 aa), FASTA scores: opt: 220, E(): 7.2e-05, (36.4% identity in 184 aa overlap). Belongs to the mpg family of DNA glycosylases.; Possible 3-methyladenine DNA glycosylase Mpg 1913590 mpg 885679 mpg Mycobacterium tuberculosis H37Rv Possible 3-methyladenine DNA glycosylase Mpg NP_216204.1 1912979 D 83332 CDS NP_216205.1 15608827 885668 1913602..1914876 1 NC_000962.3 Rv1689, (MTCI125.11), len: 424 aa. Probable tyrS,Tyrosyl-tRNA synthase, highly similar to many e.g. SYY_ECOLI|P00951 Escherichia coli (423 aa), FASTA scores: opt: 1271, E(): 0, (47.3% identity in 419 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family.; Probable tyrosyl-tRNA synthase TyrS (TYRRS) 1914876 tyrS 885668 tyrS Mycobacterium tuberculosis H37Rv Probable tyrosyl-tRNA synthase TyrS (TYRRS) NP_216205.1 1913602 D 83332 CDS NP_216206.1 15608828 885670 1915527..1915910 1 NC_000962.3 Rv1690, (MTCI125.12), len: 127 aa. Probable lprJ,lipoprotein; contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Weakly similar to other Mycobacterium tuberculosis hypothetical proteins with conserved cysteines e.g. Rv1804c, Rv1810, Rv3354, etc; Probable lipoprotein LprJ 1915910 lprJ 885670 lprJ Mycobacterium tuberculosis H37Rv Probable lipoprotein LprJ NP_216206.1 1915527 D 83332 CDS NP_216207.1 15608829 885665 1915949..1916701 1 NC_000962.3 Rv1691, MTCI125.13, len: 250 aa. Conserved hypothetical protein, similar to Q9S210|SCI51.30C|AL109848 Hypothetical protein from Streptomyces coelicolor (210 aa),FASTA score: opt: 556, E(): 6.4e-27, (50.6% identity in 180 aa overlap).; hypothetical protein 1916701 885665 Rv1691 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216207.1 1915949 D 83332 CDS NP_216208.1 15608830 885241 1916698..1917759 1 NC_000962.3 Rv1692, (MTCI125.14), len: 353 aa. Probable phosphatase, some similarity to others e.g. PNPP_SCHPO|Q00472 4-nitrophenylphosphatase (269 aa), FASTA scores: opt: 214, E(): 1.3e-10, (29.5% identity in 241 aa overlap); and to NAGD_ECOLI|P15302 nagd protein from Escherichia coli (250 aa), FASTA scores: opt: 314, E(): 9.8e-08, (28.2% identity in 245 aa overlap). Also similar to AL109848|SCI51.28 hypothetical protein from Streptomyces coelicolor (343 aa), FASTA scores: opt: 768, E(): 0, (44.8% identity in 315 aa overlap).; Probable phosphatase 1917759 885241 Rv1692 Mycobacterium tuberculosis H37Rv Probable phosphatase NP_216208.1 1916698 D 83332 CDS NP_216209.1 15608831 885662 1917756..1917932 1 NC_000962.3 Rv1693, (MTCI125.15), len: 58 aa. Conserved hypothetical protein, shows some similarity to AL583921 hypothetical protein from Mycobacterium leprae (61 aa). Probable coiled-coil from aa 30 to 58. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1917932 885662 Rv1693 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216209.1 1917756 D 83332 CDS NP_216210.1 15608832 885396 1917940..1918746 1 NC_000962.3 Rv1694, (MTCI125.16), len: 268 aa. TlyA,2'-O-methyltransferase; cytotoxin/haemolysin homologue (see citations below), almost identical to NP_301968.1|NC_002677 cytotoxin/haemolysin homologue TlyA from Mycobacterium leprae (269 aa). TlyA homologues were also identified by PCR in Mycobacterium avium, Mycobacterium bovis BCG, but appeared absent in M. smegmatis, M. vaccae, M. kansasii, M. chelonae and M. phlei (see Wren et al., 1998). Also highly similar to CAB83047.1|AJ271681 putative haemolysin from Mycobacterium ulcerans (281 aa); and similar to HLYA_TREHY|Q06803 pore-forming haemolysin/cytotoxin virulence determinant from Treponema hyodysenteriae (240 aa), FASTA scores: opt: 514, E():3e-30, (37.3% identity in 236 aa overlap).; 2'-O-methyltransferase TlyA 1918746 tlyA 885396 tlyA Mycobacterium tuberculosis H37Rv 2'-O-methyltransferase TlyA NP_216210.1 1917940 D 83332 CDS NP_216211.1 15608833 885660 1918746..1919669 1 NC_000962.3 Rv1695, (MTCI125.17), len: 307 aa. PpnK, inorganic polyphosphate/ATP-NAD kinase (see citation below),equivalent to Q49897|MLC1351.13C|Z95117|PPNK_MYCLE inorganic polyphosphate/ATP-NAD kinase from Mycobacterium leprae (311 aa) (87.9% identity in 305 aa overlap). Also similar to many e.g. P37768|PPNK_ECOLI probable inorganic polyphosphate/ATP-NAD kinase (292 aa), FASTA scores: opt: 384, E(): 1.7e-23, (33.5% identity in 233 aa overlap); etc. Belongs to the NAD kinase family.; Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase) 1919669 ppnK 885660 ppnK Mycobacterium tuberculosis H37Rv Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase) NP_216211.1 1918746 D 83332 CDS NP_216212.1 15608834 885805 1919683..1921446 1 NC_000962.3 Rv1696, (MTCI125.18), len: 587 aa. Probable recN,DNA repair protein (see citation below), similar to many e.g. RECN_ECOLI|P05824 dna repair protein recN (553 aa),FASTA scores: opt: 508, E(): 1.9e-33, (31.5% identity in 587 aa overlap). Equivalent to Z95117|MLCB1351_12 recN from Mycobacterium leprae (587 aa), FASTA scores: (76.1% identit y in 589 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable DNA repair protein RecN (recombination protein N) 1921446 recN 885805 recN Mycobacterium tuberculosis H37Rv Probable DNA repair protein RecN (recombination protein N) NP_216212.1 1919683 D 83332 CDS NP_216213.1 15608835 885045 1921542..1922723 1 NC_000962.3 Rv1697, (MTCI125.19), len: 393 aa. Conserved hypothetical protein, highly similar to Q49895|MLC1351.11C|U00021 Hypothetical protein of Mycobacterium leprae from cosmid L247 (430 aa), FASTA scores: opt: 2345, E(): 0, (90.6% identity in 393 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1922723 885045 Rv1697 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216213.1 1921542 D 83332 CDS NP_216214.1 15608836 885047 1922745..1923689 1 NC_000962.3 Rv1698, (MTCI125.20), len: 314 aa. MctB,mycobacterial copper transport protein B essential for Cu resistance and maintenance of low intracellular Cu levels (See Wolschendorf et al., 2011). Outer membrane protein (See Siroy et al., 2008) with predicted N-terminal signal sequence. Probable coiled-coil from aa 31 to 67. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Outer membrane protein MctB 1923689 mctB 885047 mctB Mycobacterium tuberculosis H37Rv Outer membrane protein MctB NP_216214.1 1922745 D 83332 CDS NP_216215.1 15608837 885048 1923829..1925589 1 NC_000962.3 Rv1699, (MTCI125.21), len: 586 aa. Probable pyrG,CTP synthase highly similar to many e.g. PYRG_ECOLI|P08398 ctp synthase from Escherichia coli (544 aa), FASTA scores: opt: 1786, E():0, (51.8% identity in 548 aa overlap). Contains PS00442 Glutamine amidotransferases class-I active site.; Probable CTP synthase PyrG 1925589 pyrG 885048 pyrG Mycobacterium tuberculosis H37Rv Probable CTP synthase PyrG NP_216215.1 1923829 D 83332 CDS NP_216216.1 15608838 885049 1925582..1926205 1 NC_000962.3 Rv1700, (MTCI125.22), len: 207 aa. Nudix hydrolase,equivalent to Q49891|MLC1351.08C|Z95117 Hypothetical protein from Mycobacterium leprae (177 aa), FASTA scores: (66.7% identity in 171 aa overlap); also similar to Q9S225|SCI51.15C|AL109848 Hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 508,E(): 1.2e-27, (43.1% identity in 197 aa overlap); similar to P54570|ADPP_BACSU ADP-ribose pyrophosphatase (185 aa),FASTA scores: opt: 313, E(): 1.1e-06, (42.7% identity in 124 aa overlap). Belongs to the family of Nudix hydrolases; NUDIX hydrolase 1926205 885049 Rv1700 Mycobacterium tuberculosis H37Rv NUDIX hydrolase NP_216216.1 1925582 D 83332 CDS NP_216217.1 15608839 885055 1926202..1927137 1 NC_000962.3 Rv1701, (MTCI125.23), len: 311 aa. Probable integrase/recombinase, similar to many e.g. XERD_ECOLI|P21891 integrase/recombinase xerd (298 aa),FASTA scores: opt: 583, E(): 0, (41.8% identity in 311 aa overlap). Also similar to other Mycobacterium tuberculosis integrase/recombinase proteins RV2894c|MTCY274.25c (43.1% identity in 304 aa overlap); and Rv2646|MTCY441.16 phiRv2 integrase (31.1% identity in 161 aa overlap). Equivalent to Z95117|MLCB1351_7 from Mycobacterium leprae (316 aa) (85.4% identity in 316 aa overlap).; Probable integrase/recombinase 1927137 885055 Rv1701 Mycobacterium tuberculosis H37Rv Probable integrase/recombinase NP_216217.1 1926202 D 83332 CDS NP_216218.1 15608840 885061 complement(1927211..1928575) 1 NC_000962.3 Rv1702c, (MTCI125.24c), len: 454 aa. Conserved hypothetical ORF in REP13E12 degenerate repeat. Similar to other hypothetical proteins inside REP13E12 elements (often in two parts) e.g. Rv0094c|Q50655|MTCY251.13c (317 aa),FASTA scores: opt: 1284, E(): 0, (59.7% identity in 315 aa overlap); and Rv1128c, Rv1945, Rv1148c, etc.; hypothetical protein 1928575 885061 Rv1702c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216218.1 1927211 R 83332 CDS NP_216219.3 448824762 885066 complement(1929131..1929721) 1 NC_000962.3 Rv1703c, (MTCI125.25c), len: 196 aa. Probable catechol-o-methyltransferase, most similar to COMT_HUMAN|P21964 soluble form of mammalian catechol o-methyltransferase (271 aa), FASTA scores: opt: 405, E(): 7.8e-29, (38.9% identity in 190 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical methyltransferases Rv0187, Rv1220c.; Probable catechol-O-methyltransferase 1929721 885066 Rv1703c Mycobacterium tuberculosis H37Rv Probable catechol-O-methyltransferase NP_216219.3 1929131 R 83332 CDS NP_216220.1 15608842 888812 complement(1929786..1931456) 1 NC_000962.3 Rv1704c, (MTCI125.26c), len: 556 aa. Probable cycA,D-serine/D-alanine/glycine transporter, highly similar to P39312|CYCA_ECOLI d-serine/d-alanine/glycine transporter from Escherichia coli (470 aa), FASTA scores: opt: 1906,E(): 0, (59.3% identity in 459 aa overlap); etc. Also similar to other Mycobacterium tuberculosis amino-acid permeases e.g. Rv2127, Rv0346c, etc. Contains PS00218 amino acid permeases signature. Belongs to the amino acid permease family (APC family).; Probable D-serine/alanine/glycine transporter protein CycA 1931456 cycA 888812 cycA Mycobacterium tuberculosis H37Rv Probable D-serine/alanine/glycine transporter protein CycA NP_216220.1 1929786 R 83332 CDS YP_177827.1 57116898 885068 complement(1931497..1932654) 1 NC_000962.3 Rv1705c, (MTCI125.27c), len: 385 aa. PPE22, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. YX23_MYCTU|Q10813 hypothetical 41.1 kDa protein cy274.2 3 (404 aa), fasta scores: opt: 819, E(): 0, (46.2% identity in 413 aa overlap).; PPE family protein PPE22 1932654 PPE22 885068 PPE22 Mycobacterium tuberculosis H37Rv PPE family protein PPE22 YP_177827.1 1931497 R 83332 CDS YP_177828.1 57116899 885070 complement(1932694..1933878) 1 NC_000962.3 Rv1706c, (MTCI125.28c), len: 394 aa. PPE23, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. YX23_MYCTU|Q10813 hypothetical 41.1 kDa protein cy274.23 (404 aa), fasta scores: opt: 841, E(): 3.9e-31, (46.8% identity in 408 aa overlap).; PPE family protein PPE23 1933878 PPE23 885070 PPE23 Mycobacterium tuberculosis H37Rv PPE family protein PPE23 YP_177828.1 1932694 R 83332 CDS YP_177651.1 57116900 3205102 complement(1934482..1934649) 1 NC_000962.3 Rv1706A, len: 55 aa. Conserved hypothetical protein,similar to part of several probable export proteins e.g. Rv0783c|Z80226_28 from Mycobacterium tuberculosis (540 aa),FASTA scores: opt: 125, E(): 0.011, (52.85% identity in 53 aa overlap). Size difference suggests possible gene fragment.; hypothetical protein 1934649 3205102 Rv1706A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177651.1 1934482 R 83332 CDS NP_216223.1 15608845 885073 1934882..1936342 1 NC_000962.3 Rv1707, (MTCI125.29), len: 486 aa. Probable conserved transmembrane protein, possibly involved in transport of sulfate, similar to several hypothetical proteins belonging to the sulfate permease family e.g. P40877|YCHM_ECOLI hypothetical 58.4 kDa protein in pth-prsa intergenic region from Escherichia coli (550 aa), FASTA scores: opt: 486, E(): 0, (33.1% identity in 492 aa overlap). Also similar to many other Mycobacterium tuberculosis membrane proteins e.g. Rv3273, Rv1739c. Seems to belong to the SulP family.; Probable conserved transmembrane protein 1936342 885073 Rv1707 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216223.1 1934882 D 83332 CDS NP_216224.1 15608846 885082 1936360..1937316 1 NC_000962.3 Rv1708, (MTCI125.30), len: 318 aa. Putative initiation inhibitor protein, a soj-related protein probably involved in cell process, highly similar to many sporulation initiation inhibitor proteins soj e.g. P37522|SOJ_BACSU Soj protein from Bacillus subtilis (253 aa), FASTA scores: opt: 745, E(): 0, (46.0% identity in 248 aa overlap), and more weakly to various repA/para/incC proteins from various organisms e.g. Y4CK_RHISN|P55393 putative replication protein A from Rhizobium sp. (407 aa),FASTA scores: opt: 205, E(): 4e-13, (29.0% identity in 252 aa overlap). Also similar to Mycobacterium tuberculosis hyothetical proteins Rv3213c and Rv3918c.; Putative initiation inhibitor protein 1937316 885082 Rv1708 Mycobacterium tuberculosis H37Rv Putative initiation inhibitor protein NP_216224.1 1936360 D 83332 CDS NP_216225.1 15608847 885083 1937313..1938149 1 NC_000962.3 Rv1709, (MTCI125.31), len: 278 aa. Possible scpA,segregation and condensation protein, similar to e.g. P35154|YPUG_BACSU from Bacillus subtilis (251 aa), FASTA scores: opt: 271, E(): 8.2e-10, (27.0% identity in 248 aa overlap); Q9S230|SCI51.10C|AL109848 from Streptomyces coelicolor (264 aa), FASTA scores: opt: 855, E(): 0, (56.8% identity in 257 aa overlap). Equivalent to Q49888|MLC1351.05C|Z95117 from Mycobacterium leprae (268 aa), FASTA scores: (78.9% identity in 251 aa overlap).; Possible segregation and condensation protein ScpA 1938149 scpA 885083 scpA Mycobacterium tuberculosis H37Rv Possible segregation and condensation protein ScpA NP_216225.1 1937313 D 83332 CDS NP_216226.1 15608848 885116 1938146..1938841 1 NC_000962.3 Rv1710, (MTCI125.32), len: 231 aa. Possible scpB,segregation and condensation protein, similar to several hypothetical proteins e.g. P35155|YPUH_BACSU from Bacillus subtilis (197 aa), FASTA scores: opt: 339, E(): 1.3e-09,(36.0% identity in 186 aa overlap); Q9S231|SCI51.09C|AL109848 from Streptomyces coelicolor (223 aa), FASTA scores: opt: 626, E(): 0, (51.0% identity in 192 aa overlap). Equivalent to O05669|MLC1351.04C|Z95117 Hypothetical protein from Mycobacterium leprae (231 aa),FASTA scores: (77.9% identity in 231 aa overlap).; Possible segregation and condensation protein ScpB 1938841 scpB 885116 scpB Mycobacterium tuberculosis H37Rv Possible segregation and condensation protein ScpB NP_216226.1 1938146 D 83332 CDS NP_216227.1 15608849 885122 1938838..1939602 1 NC_000962.3 Rv1711, (MTCI125.33), len: 254 aa. Conserved hypothetical protein, highly similar to a large family of hypothetical proteins e.g. P37765|YCIL_ECOLI from Escherichia coli (291 aa), FASTA scores: opt: 496, E(): 1.1e-29, (41.6% identity in 250 aa overlap); 9S232|SCI51.08C|AL109848 putative pseudouridine synthase from Streptomyces coelicolor (371 aa), FASTA scores: opt: 818, E(): 0, (53.1% identity in 245 aa overlap). Equivalent to O05668|MLCB1351.03C|Z95117 Hypothetical protein from Mycobacterium leprae (256 aa), (80.5% identity in 256 aa overlap). Contains PS01149 Hypothetical yciL/yejD/yjbC family signature.; hypothetical protein 1939602 885122 Rv1711 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216227.1 1938838 D 83332 CDS NP_216228.1 15608850 885157 1939599..1940291 1 NC_000962.3 Rv1712, (MTCI125.34), len: 230 aa. cmk, cytidylate kinase, highly similar to many e.g. KCY_ECOLI|P23863 cytidylate kinase from Escherichia coli (227 aa), FASTA scores: opt: 534, E (): 0, (40.3% identity in 221 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Equivalent to Z95117|MLCB1351_2 from Mycobacterium leprae (223 aa) (73.5% identity in 226 aa overlap). Belongs to the cytidylate kinase family,subfamily 1.; Cytidylate kinase Cmk (CMP kinase) (cytidine monophosphate kinase) (ck) 1940291 cmk 885157 cmk Mycobacterium tuberculosis H37Rv Cytidylate kinase Cmk (CMP kinase) (cytidine monophosphate kinase) (ck) NP_216228.1 1939599 D 83332 CDS NP_216229.1 15608851 885086 1940288..1941679 1 NC_000962.3 Rv1713, (MTCI125.35), len: 463 aa. Probable engA,GTP-binding protein. Equivalent to Q49884|MLCB1351.01|U00021_5 probable GTP-binding protein ENGA from Mycobacterium leprae (461 aa), (88.6% identity in 463 aa overlap). And similar to many e.g. P50743|ENGA_BACSU probable GTP-binding protein ENGA from Bacillus subtilus (436 aa), FASTA scores: opt: 1077, E(): 0, (40.6% identity in 434 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the era/TRME family of GTP-binding proteins. ENGA subfamily.; Probable GTP-binding protein EngA 1941679 engA 885086 engA Mycobacterium tuberculosis H37Rv Probable GTP-binding protein EngA NP_216229.1 1940288 D 83332 CDS NP_216230.1 15608852 885159 1941853..1942665 1 NC_000962.3 Rv1714, (MTV048.01), len: 270 aa. Probable oxidoreductase similar to many e.g. AE0010|AE001021_4 Archaeoglobus fulgidus section 79 (281 aa), FASTA scores: opt: 578, E(): 3.3e-31, (38.9% identity in 265 aa overlap). Also similar to several other M. tuberculosis oxidoreductases e.g. Rv1544, etc.; Probable oxidoreductase 1942665 885159 Rv1714 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_216230.1 1941853 D 83332 CDS YP_177829.1 57116901 885160 1942659..1943573 1 NC_000962.3 Rv1715, (MTV048.02), len: 304 aa. Probable fadB3,3-hydroxybutyryl-CoA dehydrogenase, highly similar to many e.g. NP_107236.1|NC_002678 3-hydroxybutyryl-CoA dehydrogenase from Mesorhizobium loti (309 aa); NP_250319.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (509 aa); P45856|HBD_BACSU probable 3-hydroxybutyryl-CoA dehydrogenase from Bacillus subtilis (287 aa), FASTA scores: opt: 488, E(): 1.5e-24, (38.7% identity in 279 aa overlap); etc. Could belong to the 3-hydroxyacyl-CoA dehydrogenase family.; Probable 3-hydroxybutyryl-CoA dehydrogenase FadB3 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) 1943573 fadB3 885160 fadB3 Mycobacterium tuberculosis H37Rv Probable 3-hydroxybutyryl-CoA dehydrogenase FadB3 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) YP_177829.1 1942659 D 83332 CDS NP_216232.1 15608854 885162 1943576..1944406 1 NC_000962.3 Rv1716, (MTV048.03,MTCY04C12.01), len: 276 aa. Conserved hypothetical protein, shows high similarity with AF1200|O29068|AE001021_11A conserved protein of Archaeoglobus fulgidus, gp fulgidus section 7 (278 aa),FASTA scores: E(): 0, (61.8% identity in 251 a a overlap); also weak similarity to several polyketide cyclases e.g. O68500|AF048833|DPSY from Streptomyces peucetius (272 aa),FASTA scores: opt: 194, E(): 1.7e-05, (29.6% identity in 223 aa overlap).; hypothetical protein 1944406 885162 Rv1716 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216232.1 1943576 D 83332 CDS NP_216233.1 15608855 885166 1944406..1944756 1 NC_000962.3 Rv1717, (MTCY04C12.02), len: 116 aa. Conserved hypothetical protein, similar to O29060|AF1208|AE001021 Hypothetical protein from Arecheoglobus fulgidus (114 aa),FASTA scores: opt: 254, E(): 3.3e-09, (37.7% identity in 114 aa overlap).; hypothetical protein 1944756 885166 Rv1717 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216233.1 1944406 D 83332 CDS NP_216234.1 15608856 885169 1944809..1945627 1 NC_000962.3 Rv1718, (MTCY04C12.03), len: 272 aa. Conserved hypothetical protein, similar to O29058|AF1210|AE001021 Hypothetical protein from Archeoglobus (313 aa), FASTA scores: opt: 301, E(): 8e-23, (31.6% identity in 301 aa overlap).; hypothetical protein 1945627 885169 Rv1718 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216234.1 1944809 D 83332 CDS NP_216235.1 15608857 885170 1945641..1946420 1 NC_000962.3 Rv1719, (MTCY04C12.04), len: 259 aa. Probable transcriptional regulatory protein, similar to YIAJ_ECOLI|P37671 hypothetical transcriptional regulator from Escherichia coli (282 aa), FASTA scores: opt: 353,E(): 3.2e-15, (31.1% identity in 235 aa overlap). Similar to Mycobacterium tuberculosis hypothetical IclR-family transcriptional regulators Rv2989, Rv1773c. Helix-turn-helix motif from aa 34-55 (+6.94 SD).; Probable transcriptional regulatory protein 1946420 885170 Rv1719 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_216235.1 1945641 D 83332 CDS NP_216236.1 15608858 885180 complement(1947030..1947419) 1 NC_000962.3 Rv1720c, (MTCY04C12.05c), len: 129 aa. Possible vapC12, toxin, part of toxin-antitoxin (TA) operon with Rv1721c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O53610|Rv0065|MTV030.08 (133 aa), FASTA scores: E(): 1.5e-10, (39.1% identity in 128 aa overlap); P71550|Rv0960|MTCY10D7.14C (129 aa) and O06415|Rv0549c|MTCY25D10.28C (137 aa).; Possible toxin VapC12 1947419 vapC12 885180 vapC12 Mycobacterium tuberculosis H37Rv Possible toxin VapC12 NP_216236.1 1947030 R 83332 CDS NP_216237.2 57116902 885182 complement(1947416..1947643) 1 NC_000962.3 Rv1721c, (MTCY04C12.06c), len: 75 aa. Possible vapB12, antitoxin, part of toxin-antitoxin (TA) operon with Rv1720c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others from Mycobacterium tuberculosis e.g. Rv0300|MTCY63.05|O07227 conserved hypothetical protein (73 aa). Start changed since original submission.; Possible antitoxin VapB12 1947643 vapB12 885182 vapB12 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB12 NP_216237.2 1947416 R 83332 CDS NP_216238.1 15608860 885183 1947861..1949345 1 NC_000962.3 Rv1722, (MTCY04C12.07), len: 494 aa. Possible carboxylases. Weak similarity to several e.g. ACCC_BACSU|P49787 biotin carboxylase from Bacillus subtilis (448 aa), fasta scores: opt: 171, E(): 0.00021, (22.8% identity in 237 aa overlap).; Possible carboxylase 1949345 885183 Rv1722 Mycobacterium tuberculosis H37Rv Possible carboxylase NP_216238.1 1947861 D 83332 CDS NP_216239.1 15608861 885185 1949342..1950589 1 NC_000962.3 Rv1723, (MTCY04C12.08), len: 415 aa. Possible hydrolase, similar to others e.g. NYLB_FLASP|P07061 6-aminohexanoate-dimer hydrolase from Flavobacterium sp. (392 aa), FASTA scores: opt: 717, E(): 0, (35.1% identity in 396 aa overlap). Also similar to M. tuberculosis hypothetical esterases and penicillin binding proteins e.g. Rv1923, Rv1497, Rv2463, etc; Probable hydrolase 1950589 885185 Rv1723 Mycobacterium tuberculosis H37Rv Probable hydrolase NP_216239.1 1949342 D 83332 CDS NP_216240.1 15608862 885189 complement(1950632..1951051) 1 NC_000962.3 Rv1724c, (MTCY04C12.09c), len: 139 aa. Hypothetical unknown protein.; Hypothetical protein 1951051 885189 Rv1724c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216240.1 1950632 R 83332 CDS NP_216241.1 15608863 885191 complement(1951041..1951751) 1 NC_000962.3 Rv1725c, (MTCY04C12.10c), len: 236 aa. Conserved hypothetical protein, similar to other hypothetical proteins from diverse organisms e.g. P70885|U44893 ORF108 from butyrivibrio fibrisolvens, (108 aa), FASTA scores: opt: 223, E(): 2e-09, (39.1% identity in 92 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical transcriptional regulator, O05774|Rv3095|YU95_MYCTU (158 aa).; hypothetical protein 1951751 885191 Rv1725c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216241.1 1951041 R 83332 CDS NP_216242.1 15608864 885192 1951852..1953237 1 NC_000962.3 Rv1726, (MTCY04C12.11), len: 461 aa. Probable oxidoreductase, similar to HDNO_ARTOX|P08159 6-hydroxy-d-nicotine oxidase (458 aa), FASTA scores: opt: 678, E(): 0, (29.5% identity in 465 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical dehydrogenases e.g. Rv3107c, Rv1257c, etc.; Probable oxidoreductase 1953237 885192 Rv1726 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_216242.1 1951852 D 83332 CDS NP_216243.1 15608865 887422 1953270..1953839 1 NC_000962.3 Rv1727, (MTCY04C12.12), len: 189 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins P72040|Rv3773c|MTCY13D12.07C (194 aa), FASTA scores: opt: 176, E(): 2.7e-08, (31.1% identity in 180 aa overlap); and O53801|Rv0738 (182 aa).; hypothetical protein 1953839 887422 Rv1727 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216243.1 1953270 D 83332 CDS NP_216244.1 15608866 885193 complement(1953864..1954634) 1 NC_000962.3 Rv1728c, (MTCY04C12.13c), len: 256 aa. Conserved hypothetical protein, some similarity to O07246|Rv0320|MTCY63.25 possible exported protein from Mycobacterium tuberculosis (220 aa), FASTA scores: E(): 1.3e-31, (42.3% identity in 220 aa overlap). C-terminal region similar to Q9ZX60|AF068845|AF068845_17 segment of gp17 of Mycobacteriophage TM4 (1229 aa), FASTA scores: opt: 385, E(): 4.3e-17, (44.6% identity in 139 aa overlap).; hypothetical protein 1954634 885193 Rv1728c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216244.1 1953864 R 83332 CDS NP_216245.1 15608867 885194 complement(1954631..1955569) 1 NC_000962.3 Rv1729c, (MTCY04C12.14c), len: 312 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to many Mycobacterium tuberculosis proteins e.g. Q50726|Rv3399|YX99_MYCTU (348 aa), FASTA scores: opt: 1019, E(): 0, (55.7% identity in 296 aa overlap); P95074|Rv0726c (367 aa), O53795|Rv0731c (318 aa),and O53841|Rv0830 (301 aa), etc.; Possible S-adenosylmethionine-dependent methyltransferase 1955569 885194 Rv1729c Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_216245.1 1954631 R 83332 CDS NP_216246.1 15608868 885202 complement(1955692..1957245) 1 NC_000962.3 Rv1730c, (MTCY04C12.15c), len: 517 aa. Possible penicillin-binding protein, similar to others e.g. PBP4_NOCLA|Q06317 penicillin-binding protein 4 (pbp-4) from Nocardia lactamdurans (381 aa), FASTA scores: opt: 643,E(): 3.8e-32, (33.8% identity in 370 aa overlap); etc. Also similar to other Mycobacterium tuberculosis hypothetical penicillin binding proteins and esterases e.g. Rv1923,Rv1497, etc.; Possible penicillin-binding protein 1957245 885202 Rv1730c Mycobacterium tuberculosis H37Rv Possible penicillin-binding protein NP_216246.1 1955692 R 83332 CDS NP_214748.2 57116903 885204 1957677..1959233 1 NC_000962.3 Rv1731, (MTCY04C12.16), len: 518 aa. Possible gabD2,succinate-semialdehyde dehydrogenase [NADP+] dependent,similar to others e.g. GABD_ECOLI|P25526 succinate-semialdehyde dehydrogenase from Escherichia coli (482 aa), FASTA scores: opt: 870, E(): 0, (34.7% identity in 449 aa overlap); etc. Also similar to gabD1|Rv0234c|MTCY08D5.30c probable succinate-semialdehyde dehydrogenase [NADP+] dependent from Mycobacterium tuberculosis (511 aa); and other semialdehyde dehydrogenases e.g. Rv0768|aldA (489 aa), Rv2858c|aldC (455 aa), etc. Contains PS00216 Sugar transport proteins signature 1, PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family. Note that previously known as gabD1.; Possible succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD2 1959233 gabD2 885204 gabD2 Mycobacterium tuberculosis H37Rv Possible succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD2 NP_214748.2 1957677 D 83332 CDS NP_216248.1 15608870 885211 complement(1959243..1959791) 1 NC_000962.3 Rv1732c, (MTCY04C12.17c), len: 182 aa. Conserved protein, highly similar to hypothetical proteins from several organisms e.g. P73178|SLL1289|D90904 from Synechocystis (194 aa), FASTA scores: opt: 663, E(): 0,(53.1% identity in 179 aa overlap); etc.; hypothetical protein 1959791 885211 Rv1732c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216248.1 1959243 R 83332 CDS NP_216249.1 15608871 885214 complement(1959855..1960487) 1 NC_000962.3 Rv1733c, (MTCY04C12.18c), len: 210 aa. Probable conserved transmembrane protein. Similar to AL109962|SCJ1_26 hypothetical protein from Streptomyces coelicolor (193 aa), FASTA scores: opt: 287, E(): 3.8e-11,(35.2% identity in 182 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable conserved transmembrane protein 1960487 885214 Rv1733c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216249.1 1959855 R 83332 CDS NP_216250.1 15608872 885212 complement(1960774..1961016) 1 NC_000962.3 Rv1734c, (MTCY04C12.19c), len: 80 aa. Conserved hypothetical protein, similar to C-terminal region Q9Z8N2|CP0452|AE001615 Dihydrolipoamide Acetyltransferase from Chlamydia pneumoniae (429 aa), FASTA scores: opt: 138,E(): 0.0012, (26.9% identity in 78 aa overlap).; hypothetical protein 1961016 885212 Rv1734c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216250.1 1960774 R 83332 CDS NP_216251.1 15608873 885216 complement(1961291..1961788) 1 NC_000962.3 Rv1735c, (MTCY04C12.20c), len: 165 aa. Hypothetical membrane protein, similar to part of O58614|PH0884|AP000004 Hypothetical malic acid transport protein from Pyrococcus horikoshii (330 aa), FASTA scores: opt: 167, E(): 0.0003,(29.2% identity in 120 aa overlap).; Hypothetical membrane protein 1961788 885216 Rv1735c Mycobacterium tuberculosis H37Rv Hypothetical membrane protein NP_216251.1 1961291 R 83332 CDS NP_216252.1 15608874 885213 complement(1962228..1964186) 1 NC_000962.3 Rv1736c, (MTCY04C12.21c), len: 652 aa. Probable narX, nitrate reductase. Contains three domains: N-terminus (250 aa) is similar to e.g. N-terminus of NARG_ECOLI|P09152 respiratory nitrate reductase 1 alpha chain from Escherichia coli (1246 aa), FASTA scores: E(): 0, (58.6% identity in 251 aa overlap); and Rv1161|MTCI65.28|NARG probable respiratory nitrate reductase (alpha chain) from Mycobacterium tuberculosis (1232 aa). Central region (260-410 aa) is similar to Rv1163|O06561|NARJ probable respiratory nitrate reductase (delta chain) from Mycobacterium tuberculosis (201 aa), FASTA scores: E(): 0,(64.2% identity in 159 aa overlap). C-terminus (420 aa-) is similar to Rv1164|O06562|NARI probable respiratory nitrate reductase (gamma chain) from Mycobacterium tuberculosis (246 aa), FASTA scores: E(): 0, (68.6% identity in 239 aa overlap). Contains PS00551 Prokaryotic molybdopterin oxidoreductases signature 1.; Probable nitrate reductase NarX 1964186 narX 885213 narX Mycobacterium tuberculosis H37Rv Probable nitrate reductase NarX NP_216252.1 1962228 R 83332 CDS NP_216253.1 15608875 885231 complement(1964183..1965370) 1 NC_000962.3 Rv1737c, (MTCY04C12.22c), len: 395 aa. Possible narK2, nitrate/nitrite-transport integral membrane protein (see Hutter & Dick 2000), possibly member of major facilitator superfamily (MFS), similar to P46907|NARK_BACSU nitrite extrusion protein from Bacillus subtilis (395 aa),FASTA scores: opt: 742, E(): 0, (33.6% identity in 375 aa overlap); and to AL109989|SCJ12.23 hypothetical nitrate/nitrite transporter from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1181, E(): 0, (49.4% identity in 389 aa overlap).; Possible nitrate/nitrite transporter NarK2 1965370 narK2 885231 narK2 Mycobacterium tuberculosis H37Rv Possible nitrate/nitrite transporter NarK2 NP_216253.1 1964183 R 83332 CDS NP_216254.1 15608876 885215 1965657..1965941 1 NC_000962.3 Rv1738, (MTCY04C12.23), len: 94 aa. Conserved protein, similar to P71931|Rv2632c|YQ32_MYCTU Hypothetical 10.1 kDa protein from Mycobacterium tuberculosis (93 aa),FASTA scores: opt: 319, E(): 2.6e-27, (53.9% identity in 89 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008).; hypothetical protein 1965941 885215 Rv1738 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216254.1 1965657 D 83332 CDS NP_216255.1 15608877 887208 complement(1965955..1967637) 1 NC_000962.3 Rv1739c, (MTCY04C12.24c, MTCY28.01), len: 560 aa. Probable sulphate-transport transmembrane protein ABC transporter, similar to several e.g. P53392|G607186 high affinity sulphate transporter from Stylosanthes hamata (662 aa), FASTA scores: opt: 382, E(): 1.6e-16, (28.0% identity in 564 aa overlap); U59234.1|AAB88215.1 biotin carb. from Synechococcus sp. PCC 7942 (574 aa), FASTA scores: opt: 1838, E(): 0, (50.0% identity in 550 aa overlap); etc. Contains PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters), and seems to belong to the SULP family.; Probable sulphate-transport transmembrane protein ABC transporter 1967637 887208 Rv1739c Mycobacterium tuberculosis H37Rv Probable sulphate-transport transmembrane protein ABC transporter NP_216255.1 1965955 R 83332 CDS NP_216256.1 15608878 885226 1967705..1967917 1 NC_000962.3 Rv1740, (MTCY28.02-MTCY04C12.25), len: 70 aa. Possible vapB34, antitoxin, part of toxin-antitoxin (TA) operon with Rv1741, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. P96913|Rv0623|MTCY20H10.04 (84 aa), (73.5% identity in 68 aa overlap); P71998|Rv1740 (70 aa), and O07770|Rv0608 (81 aa).; Possible antitoxin VapB34 1967917 vapB34 885226 vapB34 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB34 NP_216256.1 1967705 D 83332 CDS NP_216257.1 15608879 887165 1967917..1968165 1 NC_000962.3 Rv1741, (MTCY28.03,MTCY04C12.26), len: 82 aa. Possible vapC34, toxin, part of toxin-antitoxin (TA) operon with Rv1740, contains PIN domain, see Arcus et al. 2005. Similar in N-terminus to others in Mycobacterium tuberculosis e.g. P96914|Rv0624|MTCY20H10.05 (131 aa),(80.4% identity in 56 aa overlap); P71999|Rv1741 (82 aa) and O07769|Rv0609 (133 aa).; Possible toxin VapC34 Contains PIN domain 1968165 vapC34 887165 vapC34 Mycobacterium tuberculosis H37Rv Possible toxin VapC34 Contains PIN domain NP_216257.1 1967917 D 83332 CDS NP_216258.1 15608880 885234 1968173..1968910 1 NC_000962.3 Rv1742, (MTCY28.04,MTCY04C12.27), len: 245 aa. Unknown protein.; hypothetical protein 1968910 885234 Rv1742 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216258.1 1968173 D 83332 CDS NP_216259.1 15608881 885284 1969004..1970704 1 NC_000962.3 Rv1743, (MTCY28.05,MTCY04C12.28), len: 566 aa. Probable pknE, transmembrane serine/threonine protein kinase (see citation below), similar to PKN1_MYXXA|P33973 serine/threonine-protein kinase pkn1 (693 aa), fasta scores: opt: 542, E(): 1.1e-19, (35.8% identity in 302 aa overlap). Also highly similar to K08G_MYCTU|Q11053 probable serine/threonine-protein kinase (626 aa) (59.8% identity in 381 aa overlap). Contains PS00107 Protein kinases ATP-binding region signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases.; Probable transmembrane serine/threonine-protein kinase E PknE (protein kinase E) (STPK E) 1970704 pknE 885284 pknE Mycobacterium tuberculosis H37Rv Probable transmembrane serine/threonine-protein kinase E PknE (protein kinase E) (STPK E) NP_216259.1 1969004 D 83332 CDS NP_216260.1 15608882 885235 complement(1970989..1971390) 1 NC_000962.3 Rv1744c, (MTCY28.06c), len: 133 aa. Probable membrane protein, contains four imperfect 10 aa repeats,some similarity to Q25946 (MSA-2) (fragment) from Plasmodium falciparum (205 aa), FASTA scores: opt: 145, E( ): 0.048, (52.4% identity in 63 aa overlap).; Probable membrane protein 1971390 885235 Rv1744c Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216260.1 1970989 R 83332 CDS NP_216261.1 15608883 885309 complement(1971380..1971991) 1 NC_000962.3 Rv1745c, (MTCY28.08c,MTCY04C12.29c), len: 203 aa. Probable idi, isopentenyl-diphosphate delta-isomerase,similar to Q46822|ORF_O182 from Escherichia coli (182 aa),FASTA scores: opt: 465, E(): 4.7e-25, (46.9% identity in 162 aa overlap), and to IPPI_SCHPO|Q10132 isopentenyl-diphosphate delta-isomerase from Schizosaccharomyces pombe (227 aa), FASTA scores: opt: 185,E(): 5.4e-06, (30.3% identity in 152 aa overlap). Belongs to the IPP isomerase type 1 family.; Probable isopentenyl-diphosphate delta-isomerase Idi (IPP isomerase) (isopentenyl pyrophosphate isomerase) 1971991 idi 885309 idi Mycobacterium tuberculosis H37Rv Probable isopentenyl-diphosphate delta-isomerase Idi (IPP isomerase) (isopentenyl pyrophosphate isomerase) NP_216261.1 1971380 R 83332 CDS NP_216262.1 15608884 885275 1972138..1973568 1 NC_000962.3 Rv1746, (MTCY28.09, MTCY04C12.30), len: 476 aa. pknF, transmembrane serine/threonine-protein kinase (see citations below), highly similar to KY28_MYCTU|Q10697 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (589 aa), FASTA scores: opt: 870, E(): 0,(41.6% identity in 406 aa overlap). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. Start site chosen by homology, may extend further upstream.; Anchored-membrane serine/threonine-protein kinase PknF (protein kinase F) (STPK F) 1973568 pknF 885275 pknF Mycobacterium tuberculosis H37Rv Anchored-membrane serine/threonine-protein kinase PknF (protein kinase F) (STPK F) NP_216262.1 1972138 D 83332 CDS NP_216263.1 15608885 885311 1973630..1976227 1 NC_000962.3 Rv1747, (MTCY28.10, MTCY04C12.31), len: 865 aa. Probable conserved transmembrane ATP-binding protein ABC transporter (see citation below), similar to others e.g Q55956 ABC transporter from Synechocystis sp. (790 aa),FASTA scores: opt: 738, E(): 6.3e-26, (31.6% identity in 632 aa overlap); etc. Also similar to other M. tuberculosis ABC-type transporters e.g. Rv2397c|MTCY253.24, FASTA score: (35.2% identity in 213 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable conserved transmembrane ATP-binding protein ABC transporter 1976227 885311 Rv1747 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane ATP-binding protein ABC transporter NP_216263.1 1973630 D 83332 CDS NP_216264.1 15608886 885288 1976600..1977331 1 NC_000962.3 Rv1748, (MTCY28.11, MTCY04C12.32), len: 243 aa. Unknown protein. Possibly exported protein, hydrophobic domain, TM helix aa 23-45.; hypothetical protein 1977331 885288 Rv1748 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216264.1 1976600 D 83332 CDS NP_216265.1 15608887 885322 complement(1977328..1977885) 1 NC_000962.3 Rv1749c, (MTCY28.12c-MTCY04C12.33c), len: 185 aa. Possible integral membrane protein, similar to O27914|AE000940 hypothetical protein MTH1892 from Methanobacterium thermoautotrophicum (168 aa), fasta scores: E(): 9.3e-16, (37.4% identity in 123 aa overlap).; Possible integral membrane protein 1977885 885322 Rv1749c Mycobacterium tuberculosis H37Rv Possible integral membrane protein NP_216265.1 1977328 R 83332 CDS NP_216266.1 15608888 885310 complement(1977969..1979567) 1 NC_000962.3 Rv1750c, (MTCY28.13c, MTCY04C12.34), len: 532 aa. Possible fadD1, fatty-acid-CoA synthetase, similar in part to others e.g. O35488|VLCS_MOUSE very-long-chain acyl-CoA synthetase from Mus musculus (620 aa); NP_113924.1|NM_031736 solute carrier family 27 (fatty acid transporter) member 2 from Rattus norvegicus (620 aa); NP_459076.1|NC_003197 crotonobetaine/carnitine-CoA ligase from Salmonella typhimurium (517 aa); CAIC_ECOLI|P31552 probable crotonobetaine/carnitine-CoA ligase from Escherichia coli (522 aa), FASTA scores: opt: 448, E(): 1.9e-21, (25.1% identity in 502 aa overlap); etc. Also highly similar to fadD17|Rv3506|MTV023.13 probable fatty-acid-CoA ligase from Mycobacterium tuberculosis (502 aa); and similar to others from Mycobacterium tuberculosis e.g. fadD6|MTCI364.18|Rv1206|O05307 probable fatty-acid-CoA ligase (597 aa), FASTA score: (28.3% identity in 519 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Possible fatty-acid-CoA ligase FadD1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 1979567 fadD1 885310 fadD1 Mycobacterium tuberculosis H37Rv Possible fatty-acid-CoA ligase FadD1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_216266.1 1977969 R 83332 CDS NP_216267.1 15608889 885489 1979621..1981003 1 NC_000962.3 Rv1751, (MTCY28.14-MTCY04C12.35), len: 460 aa. Probable oxidoreductase, possibly a monooxygenase or hydroxylase, similar to MHPA_ECOLI|P77397 3-(3-hydroxy-phenyl) propionate hydroxylase (554 aa), FASTA scores: opt: 239, E(): 2e-08, (24.6% identity in 435 aa overlap); and AJ007932|SAR7932.13 oxygenase from Streptomyces argillaceus (436 aa), FASTA scores: opt: 587,E(): 8.6e-30, (32.3% identity in 359 aa overlap). Contains PS00075 Dihydrofolate reductase signature. Also similar to Mycobacterium tuberculosis hypothetical oxidoreductases Rv1260 and Rv0575c.; Probable oxidoreductase 1981003 885489 Rv1751 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_216267.1 1979621 D 83332 CDS NP_216268.1 15608890 885313 1981130..1981579 1 NC_000962.3 Rv1752, (MTCY28.15), len: 149 aa. Conserved hypothetical protein, similar to C-terminal half of Q9TV68|AB021930|CAN2DD Dihydrodiol dehydrogenase from Canis familiaris (335 aa), FASTA score, opt: 168, E(): 0.00015,(31.3% identity in 112 aa overlap).; hypothetical protein 1981579 885313 Rv1752 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216268.1 1981130 D 83332 CDS YP_177830.1 57116904 885544 complement(1981614..1984775) 1 NC_000962.3 Rv1753c, (MTCY28.16c), len: 1053 aa. PPE24, Member of the Mycobacterium tuberculosis PPE family of Gly-,Asn-rich proteins, similar to many e.g. YF48_MYCTU|Q10778 hypothetical protein cy48.17 (678 aa), FASTA scores: opt: 1360, E(): 0, (48.9% identity in 550 aa overlap). Note that the Gly-, Asn-rich sequence is interrupted by six near-perfect 26 aa repeats, a unique region, and another,more degenerate region of five 25 aa repeats before resuming at the C-terminus. The end of the first Gly-, Asn-rich region and the start of the first set of repeats shows some similarity to Q50577|AT10S from Mycobacterium tuberculosis (170 aa) (40.2% identity in 189 aa overlap).; PPE family protein PPE24 1984775 PPE24 885544 PPE24 Mycobacterium tuberculosis H37Rv PPE family protein PPE24 YP_177830.1 1981614 R 83332 CDS NP_216270.1 15608892 885467 complement(1984979..1986670) 1 NC_000962.3 Rv1754c, (MTCY28.17c), len: 563 aa. Conserved protein, has proline-rich central region. Some similarity in central region to other Mycobacterium tuberculosis proline-rich proteins e.g. O06555|Rv1157c|MTCI65.24c (371 aa), (32.5% identity in 191 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 1986670 885467 Rv1754c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216270.1 1984979 R 83332 CDS NP_216271.1 15608893 885566 complement(1986854..>1987696) 1 NC_000962.3 Rv1755c, (MT1799, MTCY28.21c), len: 280 aa. Probable plcD, phospholipase C 4 (fragment) (see citations below),highly similar to C-terminus of other phospholipases e.g. CQ50771|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 from Mycobacterium tuberculosis (512 aa), FASTA score: (71.1% identity in 284 aa overlap); etc. Note that this ORF has been interrupted by insertion of IS6110 element. Belongs to the bacterial phospholipase C family.; Probable phospholipase C 4 (fragment) PlcD >1987696 plcD 885566 plcD Mycobacterium tuberculosis H37Rv Probable phospholipase C 4 (fragment) PlcD NP_216271.1 1986854 R 83332 CDS NP_216272.2 448824763 885541 complement(1987745..>1988731) 1 NC_000962.3 Rv1756c, (MTCY28.22c), len: 328 aa. Putative Transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1756c and Rv1757c, the sequence UUUUAAAG (directly upstream of Rv1756c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa); Putative transposase >1988731 885541 Rv1756c Mycobacterium tuberculosis H37Rv Putative transposase NP_216272.2 1987745 R 83332 CDS NP_216273.1 15608895 885558 complement(1988680..1989006) 1 NC_000962.3 Rv1757c, (MTCY28.23c), len: 108 aa. Putative Transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43,(100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1756c and Rv1757c, the sequence UUUUAAAG (directly upstream of Rv1756c) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Putative transposase for insertion sequence element IS6110 (fragment) 1989006 885558 Rv1757c Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 (fragment) NP_216273.1 1988680 R 83332 CDS NP_216274.1 15608896 885552 1989042..1989566 1 NC_000962.3 Rv1758, (MTCY28.24), len: 174 aa. Probable cut1,serine esterase, cutinase family, similar to Rv2301|CUT2_MYCTU|Q50664 probable cutinase cy339.08c precursor from Mycobacterium tuberculosis (219 aa), FASTA scores: opt: 369, E(): 1. 1e-16, (39.1% identity in 179 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical cutinases Rv3452, Rv1984c, Rv3451 and Rv3724. CDS has been interrupted by IS6110 insertion element and 5'-end deleted. Belongs to the cutinase family.; Probable cutinase Cut1 1989566 cut1 885552 cut1 Mycobacterium tuberculosis H37Rv Probable cutinase Cut1 NP_216274.1 1989042 D 83332 CDS YP_177831.1 57116905 885325 complement(1989833..1992577) 1 NC_000962.3 Rv1759c, (MT1807, MTCY28.25c), len: 914 aa. Wag22,antigen member (see citations below) of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, highly similar to others e.g. MT1367|Q10637 hypothetical glycine-rich 49.6 kDa protein from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 2010, E(): 0, (53.0% identity in 724 aa overlap); etc.; PE-PGRS family protein Wag22 1992577 wag22 885325 wag22 Mycobacterium tuberculosis H37Rv PE-PGRS family protein Wag22 YP_177831.1 1989833 R 83332 CDS NP_216276.1 15608898 885556 1993153..1994661 1 NC_000962.3 Rv1760, (MTCY28.26), len: 502 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to several other Mycobacterium tuberculosis proteins e.g. Q10554|Y895_MYCTU|MTCY31.23 (505 aa), FASTA scores: opt: 692, E(): 0, (31.7% identity in 477 aa overlap). Member of family with at least 15 other members e.g. Rv3740c,Rv3734c, Rv1425, etc.; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 1994661 885556 Rv1760 Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_216276.1 1993153 D 83332 CDS NP_216277.1 15608899 885436 complement(1994671..1995054) 1 NC_000962.3 Rv1761c, (MTCY28.27c), len: 127 aa. Possibly exported protein with hydrophobic stretch or TMhelix at aa 15-37.; Possible exported protein 1995054 885436 Rv1761c Mycobacterium tuberculosis H37Rv Possible exported protein NP_216277.1 1994671 R 83332 CDS NP_216278.1 15608900 885373 complement(1995054..1995842) 1 NC_000962.3 Rv1762c, (MTCY28.28c), len: 262 aa. Unknown protein.; hypothetical protein 1995842 885373 Rv1762c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216278.1 1995054 R 83332 CDS NP_216279.1 15608901 885372 1996152..1996478 1 NC_000962.3 Rv1763, (MTCY28.29), len: 108 aa. Putative Transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43,(100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1763 and Rv1764, the sequence UUUUAAAG (directly upstream of Rv1764) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Putative transposase for insertion sequence element IS6110 (fragment) 1996478 885372 Rv1763 Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 (fragment) NP_216279.1 1996152 D 83332 CDS NP_216280.2 448824764 885238 <1996427..1997413 1 NC_000962.3 Rv1764, (MTCY28.30), len: 328 aa. Putative Transposase for IS6110 insertion element. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1763 and Rv1764,the sequence UUUUAAAG (directly upstream of Rv1764) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa); Putative transposase 1997413 885238 Rv1764 Mycobacterium tuberculosis H37Rv Putative transposase NP_216280.2 <1996427 D 83332 CDS NP_216281.1 15608903 885256 complement(1997418..1998515) 1 NC_000962.3 Rv1765c, (MTCY28.31c), len: 365 aa. Conserved hypothetical protein, highly similar to O53461|Rv2015c|MTV018.02c conserved hypothetical protein (418 aa), (97.8% identity in 364 aa overlap). Blast hits with non-is part of sequence submitted under MTU78639.; hypothetical protein 1998515 885256 Rv1765c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216281.1 1997418 R 83332 CDS YP_177652.1 57116906 3205098 complement(1999142..1999357) 1 NC_000962.3 Rv1765A, len: 71 aa. Putative transposase (fragment), similar to part of many transposase genes including IS6110 e.g. P19774|TRA9_MYCTU putative transposase from Mycobacterium tuberculosis (278 aa), FASTA scores: opt: 231, E(): 4.7e-11, (45.35% identity in 75 aa overlap).; Putative transposase (fragment) 1999357 3205098 Rv1765A Mycobacterium tuberculosis H37Rv Putative transposase (fragment) YP_177652.1 1999142 R 83332 CDS NP_216282.2 57116907 885427 1999737..2000006 1 NC_000962.3 Rv1766, (MTCY28.32), len: 89 aa. Conserved protein,highly similar to P54431|YRKD_BACSU Hypothetical 7.0 kDa protein in bltr-spoIIIC intergenic region from Bacillus subtilis (63 aa), FASTA scores: opt: 151, E(): 1.5e-05,(53.3% identity in 45 aa overlap). Also similar to Q9RD62|SCF56.04C|AL133424 Hypothetical protein from Streptomyces coelicolor (92 aa), FASTA scores: opt: 239,E(): 1.3e-11, (62.5% identity in 64 aa overlap). Also some similarity to other Mycobacterium tuberculosis hypothetical proteins e.g. O07434|Rv0190|MTCI28.29 (96 aa), (35.5% identity in 62 aa overlap); P71543|Rv0967 (119 aa), and P71600|Rv0030 (109 aa). Start changed since original submission.; hypothetical protein 2000006 885427 Rv1766 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216282.2 1999737 D 83332 CDS NP_216283.1 15608905 885443 2000074..2000433 1 NC_000962.3 Rv1767, (MTCY28.33), len: 119 aa. Conserved protein,similar to Q57498|YA53_HAEIN hypothetical protein HI1053 from Haemophilus influenzae (113 aa), FASTA scores: opt: 233, E(): 6.4e-10, (40.0% identity in 90 aa overlap).; hypothetical protein 2000433 885443 Rv1767 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216283.1 2000074 D 83332 CDS YP_177832.1 57116908 885429 2000614..2002470 1 NC_000962.3 Rv1768, (MTCY28.34), len: 618 aa. PE_PGRS31, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to Q50615 hypothetical 40.8 kDa protein (498 aa),FASTA scores: opt: 1703, E(): 0, (57.4% identity in 566 aa overlap).; PE-PGRS family protein PE_PGRS31 2002470 PE_PGRS31 885429 PE_PGRS31 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS31 YP_177832.1 2000614 D 83332 CDS NP_216285.1 15608907 885420 2002626..2003870 1 NC_000962.3 Rv1769, (MTCY28.35), len: 414 aa. Conserved protein,similar to O88066|SCI35.31|AL031541 hypothetical protein from Streptomyces coelicolor (402 aa), FASTA scores: opt: 1341, E(): 0, (53.8% identity in 398 aa overlap).; hypothetical protein 2003870 885420 Rv1769 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216285.1 2002626 D 83332 CDS NP_216286.1 15608908 885415 2003878..2005164 1 NC_000962.3 Rv1770, (MTCY28.36), len: 428 aa. Conserved protein,highly similar in N-terminus to Q49882 Hypothetical protein from Mycobacterium leprae from cosmid L247 (83 aa), FASTA scores: opt: 301, E(): 1e-12, (56.5% identity in 85 aa overlap).; hypothetical protein 2005164 885415 Rv1770 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216286.1 2003878 D 83332 CDS NP_216287.1 15608909 885441 2005161..2006447 1 NC_000962.3 Rv1771, (MTCY28.37), len: 428 aa. L-gulono-1,4-lactone dehydrogenase (See Wolucka and Communi, 2006), similar to e.g. GGLO_RAT|P10867 l-gulonolactone oxidase (439 aa), FASTA scores: opt: 862,E(): 0, (34.1% identity in 434 aa overlap). Also shows slight similarity to Mycobacterium tuberculosis oxidoreductase Rv1726|MTCY04C12.11 (22.9% identity in 441 aa overlap) and others e.g. Rv3107c, Rv1257c, Rv2251, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. Alternative nucleotide at position 2006032 (a->G; Q291R) has been observed.; L-gulono-1,4-lactone dehydrogenase 2006447 885441 Rv1771 Mycobacterium tuberculosis H37Rv L-gulono-1,4-lactone dehydrogenase NP_216287.1 2005161 D 83332 CDS NP_216288.1 15608910 885920 2006636..2006947 1 NC_000962.3 Rv1772, (MTCY28.38), len: 103 aa. Hypothetical unknown protein.; Hypothetical protein 2006947 885920 Rv1772 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216288.1 2006636 D 83332 CDS NP_216289.1 15608911 885342 complement(2007020..2007766) 1 NC_000962.3 Rv1773c, (MTCY28.39), len: 248 aa. Probable transcriptional regulator belonging to IclR family, similar to ICLR_ECOLI|P16528 acetate operon repressor from Escherichia coli (274 aa), FASTA scores: opt: 261, E(): 3.3e-10, (26.9% identity in 249 aa overlap). Also similar to Mycobacterium tuberculosis protein Rv1719|MTCY04C12.04 (40.2% identity in 244 aa overlap); and Rv2989. Start site chosen by homology, but may extend further upstream. Contains possible helix-turn-helix motif at aa 37-58 (+3.24 SD).; Probable transcriptional regulatory protein 2007766 885342 Rv1773c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_216289.1 2007020 R 83332 CDS NP_216290.1 15608912 885367 2007832..2009172 1 NC_000962.3 Rv1774, (MTCY25C11.01), len: 446 aa. Probable oxidoreductase, similar to several e.g. HDNO_ARTOX|P08159 6-hydroxy-d-nicotine oxidase (458 aa), FASTA scores: opt: 417, E(): 6e-20, (28.4% identity in 462 aa overlap). Also some similarity to Mycobacterium tuberculosis oxidoreductase MTCY04C12.11 (24.1% identity in 444 aa overlap). Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site.; Probable oxidoreductase 2009172 885367 Rv1774 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_216290.1 2007832 D 83332 CDS NP_216291.1 15608913 885842 2009172..2009990 1 NC_000962.3 Rv1775, (MTCY25C11.02), unknown, len: 272 aa. Conserved hypothetical protein, similar to O28806|AF1466 conserved hypothetical protein from Archaeoglobus fulgidus (255 aa), FASTA scores: opt: 364, E(): 1e-17, (29.2% identity in 267 aa overlap).; hypothetical protein 2009990 885842 Rv1775 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216291.1 2009172 D 83332 CDS NP_216292.1 15608914 885821 complement(2009995..2010555) 1 NC_000962.3 Rv1776c, (MTCY25C11.03c), len: 186 aa. Possible regulatory protein, some similarity to Mycobacterium tuberculosis Rv1255c|Q11063 hypothetical transcriptional regulator (202 aa), FASTA scores: opt: 270, E(): 9.7e-09,(28.3% identity in 191 aa overlap). Contains possible helix-turn-helix motif at aa 37-58 (+3.49 SD).; Possible transcriptional regulatory protein 2010555 885821 Rv1776c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_216292.1 2009995 R 83332 CDS NP_216293.1 15608915 885839 2010656..2011960 1 NC_000962.3 Rv1777, (MT1827, MTCY25C11.04), len: 434 aa. Probable cyp144, cytochrome p450, similar to CPXM_BACME|Q06069 cytochrome p450 (meg) (410 aa), FASTA scores: opt: 435 E(): 2.3e-16, (28.8% identity in 372 aa overlap). Also similar to several other Mycobacterium tuberculosis p450 genes including Rv0766c, Rv2266, etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Probable cytochrome P450 144 Cyp144 2011960 cyp144 885839 cyp144 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 144 Cyp144 NP_216293.1 2010656 D 83332 CDS NP_216294.1 15608916 885424 complement(2012081..2012530) 1 NC_000962.3 Rv1778c, (MTCY25C11.05c), len: 149 aa. Unknown protein.; hypothetical protein 2012530 885424 Rv1778c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216294.1 2012081 R 83332 CDS NP_216295.1 15608917 885829 complement(2012686..2014479) 1 NC_000962.3 Rv1779c, (MTV049.01c), len: 597 aa. Possible integral membrane protein.; Possible integral membrane protein 2014479 885829 Rv1779c Mycobacterium tuberculosis H37Rv Possible integral membrane protein NP_216295.1 2012686 R 83332 CDS NP_216296.1 15608918 885851 2014699..2015262 1 NC_000962.3 Rv1780, (MTV049.02), len: 187 aa. Conserved protein,equivalent to Q49881|ML1380|U00021_2 cosmid L247 from Mycobacterium leprae (187 aa), FASTA scores: opt: 1000,E(): 0, (82.4% identity in 187 aa overlap).; hypothetical protein 2015262 885851 Rv1780 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216296.1 2014699 D 83332 CDS NP_216297.1 15608919 885854 complement(2015302..2017476) 1 NC_000962.3 Rv1781c, (MTV049.03c), len: 724 aa. Probable malQ,4-alpha-glucanotransferase, similar to many, e.g. P15977|MALQ_ECOLI 4-alpha-glucanotransferase (694 aa),FASTA scores: opt: 964, E(): 0, (31.8% identity in 694 aa overlap). Belongs to the disproportionating enzyme family.; Probable 4-alpha-glucanotransferase MalQ (amylomaltase) (disproportionating enzyme) (D-enzyme) 2017476 malQ 885854 malQ Mycobacterium tuberculosis H37Rv Probable 4-alpha-glucanotransferase MalQ (amylomaltase) (disproportionating enzyme) (D-enzyme) NP_216297.1 2015302 R 83332 CDS NP_216298.1 15608920 885347 2017740..2019260 1 NC_000962.3 Rv1782, (MTV049.04), len: 506 aa. eccB5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, similar to four other Mycobacterium tuberculosis hypothetical membrane proteins e.g. O05449|Rv3895c|MTCY15F10.17|Z94121 (495 aa), FASTA scores: opt: 1106, E(): 0, (41.2% identity in 485 aa overlap); Rv0283, Rv3450c, and Rv3869, all located near ESAT-6 family genes. Also similar to O33088|MLCB628.17C|Y14967 cosmid B628 from Mycobacterium leprae (481 aa), (32.7% identity in 486 aa overlap); and equivalent to Q9Z5I3|MLCB596.27|AL035472 hypothetical protein from Mycobacterium leprae (506 aa) (82.6% identity in 506 aa overlap). Has hydrophobic stretch from aa 54-76. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX conserved component EccB5 ESX-5 type VII secretion system protein Probable membrane protein 2019260 eccB5 885347 eccB5 Mycobacterium tuberculosis H37Rv ESX conserved component EccB5 ESX-5 type VII secretion system protein Probable membrane protein NP_216298.1 2017740 D 83332 CDS YP_007410461.1 448816588 885898 2019257..2023432 1 NC_000962.3 Rv1783, (MTV049.05-MTV049.06), len: 1391 aa. eccC5,esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein. FtsK/SpoIIIE family protein. Similar to Rv3894c. Member of family of Mycobacterium tuberculosis hypothetical proteins including Rv3447c, Rv0284, Rv3870, Rv1783, Rv3871, Rv3894c, all linked to ESAT-6 family genes. Equivalent to Mycobacterium leprae hypothetical protein Q9Z512|MLCB596.28|AL035472 (1345 aa). Previously annotated as two separate genes eccCa5|Rv1783 and eccCb5|Rv1784, now fused due to A:T correction at position 2020563 resulting in *463L. Contains two times PS00017 ATP/GTP-binding site motif A (P-loop). Former Rv1784 - Predicted to be an outer membrane protein (See Song et al., 2008).; ESX conserved component EccC5 ESX-5 type VII secretion system protein 2023432 eccC5 885898 eccC5 Mycobacterium tuberculosis H37Rv ESX conserved component EccC5 ESX-5 type VII secretion system protein YP_007410461.1 2019257 D 83332 CDS NP_216301.1 15608923 885907 complement(2023447..2024628) 1 NC_000962.3 Rv1785c, (MT1834, MTV049.07c), len: 393 aa. Probable cyp143, cytochrome P450 (1.14.-.-), similar to many e.g. AE0001|RZAE000101_4 Rhizobium sp. NGR234 (414 aa), FASTA scores: opt: 663, E(): 0, (32.4% identity in 413 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Probable cytochrome P450 143 Cyp143 2024628 cyp143 885907 cyp143 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 143 Cyp143 NP_216301.1 2023447 R 83332 CDS NP_216302.1 15608924 885846 2024828..2025031 1 NC_000962.3 Rv1786, (MTV049.08), len: 67 aa. Probable ferredoxin, similar to others e.g. X63601|FERS_STRGR ferredoxin from Streptomyces griseus (65 aa), FASTA scores: opt: 140, E(): 0.001, (38.1% identity in 63 aa overlap); T50943 probable ferredoxin DitA from Pseudomonas abietaniphila (78 aa); BAA84714.1|AB017795 ferredoxin from Nocardioides sp. (69 aa); etc. Also similar to Rv0763c|MTCY369.08 from Mycobacterium tuberculosis (68 aa),FASTA score: (30.6% identity in 62 aa overlap); and Rv0763c.; Probable ferredoxin 2025031 885846 Rv1786 Mycobacterium tuberculosis H37Rv Probable ferredoxin NP_216302.1 2024828 D 83332 CDS YP_177833.1 57116909 885827 2025301..2026398 1 NC_000962.3 Rv1787, (MTV049.09), len: 365 aa. PPE25, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to Z74024|MTCY274.24 Mycobacterium tuberculosis cosmid (404 aa), FASTA scores: opt: 837, E(): 0, (52.0% identity in 406 aa overlap).; PPE family protein PPE25 2026398 PPE25 885827 PPE25 Mycobacterium tuberculosis H37Rv PPE family protein PPE25 YP_177833.1 2025301 D 83332 CDS YP_177834.1 57116910 885895 2026477..2026776 1 NC_000962.3 Rv1788, (MTV049.10), len: 99 aa. PE18, Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), similar to Z93777|MTCI364.07 Mycobacterium tuberculosis cosmid (99 aa), FASTA scores: opt: 414, E(): 3.6e-20, (72.4% identity in 98 aa overlap).; PE family protein PE18 2026776 PE18 885895 PE18 Mycobacterium tuberculosis H37Rv PE family protein PE18 YP_177834.1 2026477 D 83332 CDS YP_177835.1 57116911 885333 2026790..2027971 1 NC_000962.3 Rv1789, (MTV049.11), len: 393 aa. PPE26, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g.Z98268|MTCI125.26 Mycobacterium tuberculosis cosmid (385 aa), FASTA score: opt: 1283, E(): 0, (62.7% identity in 408 aa overlap).; PPE family protein PPE26 2027971 PPE26 885333 PPE26 Mycobacterium tuberculosis H37Rv PPE family protein PPE26 YP_177835.1 2026790 D 83332 CDS YP_177836.1 57116912 885859 2028425..2029477 1 NC_000962.3 Rv1790, (MTV049.12), len: 350 aa. PPE27, Member of the Mycobacterium tuberculosis PPE family of glycine-rich protein, similar to Z74024|MTCY274.24 Mycobacterium tuberculosis cosmid (404 aa), FASTA scores: opt: 849, E(): 0, (50.0% identity in 406 aa overlap).; PPE family protein PPE27 2029477 PPE27 885859 PPE27 Mycobacterium tuberculosis H37Rv PPE family protein PPE27 YP_177836.1 2028425 D 83332 CDS YP_177837.1 57116913 885445 2029904..2030203 1 NC_000962.3 Rv1791, (MTV049.13), len: 99 aa. PE19, Member of the Mycobacterium tuberculosis PE family, but no glycine rich C-terminus (see Brennan & Delogu 2002), highly similar to Z93777|MTCI364.07 M.tuberculosis cosmid (99 aa) opt: 430 E(): 2.4e-21, (75.5% identity in 98 aa overlap).; PE family protein PE19 2030203 PE19 885445 PE19 Mycobacterium tuberculosis H37Rv PE family protein PE19 YP_177837.1 2029904 D 83332 CDS YP_177838.1 57116914 885448 2030694..2030978 1 NC_000962.3 Rv1793, (MT1842, MTV049.15), len: 94 aa. EsxN,ESAT-6 like protein (see citation below), almost identical to several mycobacterial proteins of the ESAT-6-like family including P95242|Rv2346c|MTCY98.15C|Z83860 putative ESAT-6 like protein 6 (94 aa), FASTA scores: opt: 610, E(): 0,(97.9 % identity in 94 aa overlap); Rv3619c, Rv1037c, and Rv1198, etc. Also present in Mycobacterium leprae. Seems to belong to the ESAT6 family. Predicted possible vaccine candidate (See Zvi et al., 2008).; Putative ESAT-6 like protein EsxN (ESAT-6 like protein 5) 2030978 esxN 885448 esxN Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxN (ESAT-6 like protein 5) YP_177838.1 2030694 D 83332 CDS NP_216310.1 15608931 885881 2031066..2031968 1 NC_000962.3 Rv1794, (MTV049.16), len: 300 aa. Conserved protein,slight similarity to Mycobacterium tuberculosis O53694|Rv0289|MTV035.17, (295 aa), FASTA scores: opt: 172,E(): 0.00083, (25.7% identity in 261 aa overlap). Equivalent to Mycobacterium leprae hypothetical protein Q9Z5I1|MLCB596.31|AL035472 (300 aa), (88.0% identity in 300 aa overlap). Contains PS00211 ABC transporters family signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2031968 885881 Rv1794 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216310.1 2031066 D 83332 CDS NP_216311.1 15608932 885628 2032240..2033751 1 NC_000962.3 Rv1795, (MTV049.17), len: 503 aa. eccD5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, has a hydrophilic stretch from ~1-130 then very hydrophobic. Similar to several other mycobacterial proteins, all linked to ESAT-6 family e.g. Rv3887c|MTY15F10.24|Z94121 (509 aa), FASTA scores: opt: 360, E(): 1.6e-15, (26.7% identity in 514 aa overlap); Rv3448, and Rv0290.; ESX conserved component EccD5 ESX-5 type VII secretion system protein Probable membrane protein 2033751 eccD5 885628 eccD5 Mycobacterium tuberculosis H37Rv ESX conserved component EccD5 ESX-5 type VII secretion system protein Probable membrane protein NP_216311.1 2032240 D 83332 CDS NP_216312.1 15608933 885879 2033729..2035486 1 NC_000962.3 Rv1796, (MTV049.18), len: 585 aa. Probable mycP5,pro-rich membrane-anchored serine protease (mycosin) (see citations below). Member of family with four other Mycobacterium tuberculosis serine proteases: Rv3886c|O05458|MTCY15F10.26|Z94121 (550 aa), FASTA scores: opt: 1173, E(): 0, (47.9% identity in 578 aa overlap); Rv0291, Rv3883c, and Rv3449. Genes all linked to those of ESAT-6 family. Has possible N-terminal signal peptide and hydrophobic anchor-like stretch at C-terminus. Contains two serine protease, subtilase family active site motifs: a aspartic acid active site motif (PS00136); and a histidine active site motif (PS00137). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable proline rich membrane-anchored mycosin MycP5 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-5) 2035486 mycP5 885879 mycP5 Mycobacterium tuberculosis H37Rv Probable proline rich membrane-anchored mycosin MycP5 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-5) NP_216312.1 2033729 D 83332 CDS NP_216313.1 15608934 885452 2035483..2036703 1 NC_000962.3 Rv1797, (MTV049.19), len: 406 aa. eccE5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, some similarity to Mycobacterium tuberculosis O05462|Rv3882c|MTCY15F10.30|Z94121 (462 aa), FASTA scores: opt: 181, E(): 9.2e-05, (25.4% identity in 283 aa overlap). Has two hydrophobic stretch near N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX conserved component EccE5 ESX-5 type VII secretion system protein Probable membrane protein 2036703 eccE5 885452 eccE5 Mycobacterium tuberculosis H37Rv ESX conserved component EccE5 ESX-5 type VII secretion system protein Probable membrane protein NP_216313.1 2035483 D 83332 CDS NP_216314.1 15608935 885543 2036700..2038532 1 NC_000962.3 Rv1798, (MTV049.20), len: 610 aa. eccA5, esx conserved component, ESX-5 type VII secretion system protein, similar to several mycobacterial proteins e.g. O05460|MTCY15F10.28|Rv3884c|Z94121 from M. tuberculosis (619 aa), FASTA scores: opt: 669, E(): 0, (31.0% identity in 549 aa overlap); and O33089|MLCB628.18c|Y14967 from Mycobacterium leprae (573 aa), FASTA scores: opt: 723, E(): 0, (32.4% identity in 568 aa overlap). Also very similar to Rv0282. May belong to the CbxX/CfqX family as last ~320 aa domain very similar to several family members. Contains ATP/GTP-binding site motif A (P-loop; PS00017).; ESX conserved component EccA5 ESX-5 type VII secretion system protein 2038532 eccA5 885543 eccA5 Mycobacterium tuberculosis H37Rv ESX conserved component EccA5 ESX-5 type VII secretion system protein NP_216314.1 2036700 D 83332 CDS NP_216315.1 15608936 885901 2039159..2039350 1 NC_000962.3 Rv1799, (MTV049.21), len: 63 aa. Probable lppT lipoprotein, has possible signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LppT 2039350 lppT 885901 lppT Mycobacterium tuberculosis H37Rv Probable lipoprotein LppT NP_216315.1 2039159 D 83332 CDS YP_177839.1 57116915 885465 2039453..2041420 1 NC_000962.3 Rv1800, (MTV049.22), len: 655 aa. PPE28, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, C-terminal very similar to parts of PE proteins e.g. Z92770|MTCI5.25|Rv0151c (588 aa), FASTA scores: opt: 1269, E(): 0, (41.5% identity in 591 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE28 2041420 PPE28 885465 PPE28 Mycobacterium tuberculosis H37Rv PPE family protein PPE28 YP_177839.1 2039453 D 83332 CDS YP_177840.1 57116916 885491 2042001..2043272 1 NC_000962.3 Rv1801, (MTV049.23), len: 423 aa. PPE29, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to AL022021|MTV049.29|Rv1808 (409 aa), FASTA scores: opt: 1229, E(): 0, (55.2% identity in 422 aa overlap).; PPE family protein PPE29 2043272 PPE29 885491 PPE29 Mycobacterium tuberculosis H37Rv PPE family protein PPE29 YP_177840.1 2042001 D 83332 CDS YP_177841.1 57116917 885542 2043384..2044775 1 NC_000962.3 Rv1802, (MTV049.24), len: 463 aa. PPE30, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to AL022021|MTV049.30|Rv1809 (468 aa), FASTA scores: opt: 1238, E(): 0, (51.0% identity in 471 aa overlap).; PPE family protein PPE30 2044775 PPE30 885542 PPE30 Mycobacterium tuberculosis H37Rv PPE family protein PPE30 YP_177841.1 2043384 D 83332 CDS YP_177842.1 57116918 885730 complement(2044923..2046842) 1 NC_000962.3 Rv1803c, (MTV049.25c), len: 639 aa. PE_PGRS32,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Most similar to Rv1768|MTCY28.34|Z95890 (618 aa), FASTA scores: opt: 1827, E(): 0, (53.5% identity in 664 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures 1. Predicted to be an outer membrane protein (See Song et al., 2008).; PE-PGRS family protein PE_PGRS32 2046842 PE_PGRS32 885730 PE_PGRS32 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS32 YP_177842.1 2044923 R 83332 CDS NP_216320.1 15608941 885588 complement(2047023..2047349) 1 NC_000962.3 Rv1804c, (MTV049.26c), len: 108 aa. Conserved protein, similar to several hypothetical Mycobacterium tuberculosis proteins that may be exported (hydrophobic stretch at N-terminus) e.g. O07222|Rv1810|MTCY16F9.04C|Z96073 (118 aa), FASTA scores: opt: 361, E(): 2.3e-19, (53.5% identity in 101 aa overlap); Rv0622, Rv1690, and Rv3067, etc. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 2047349 885588 Rv1804c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216320.1 2047023 R 83332 CDS NP_216321.1 15608942 885470 complement(2047687..2048034) 1 NC_000962.3 Rv1805c, (MTV049.27c), len: 115 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2048034 885470 Rv1805c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216321.1 2047687 R 83332 CDS YP_177843.1 57116919 885537 2048072..2048371 1 NC_000962.3 Rv1806, (MTV049.28), len: 99 aa. PE20, Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334,E(): 4.7 e-15, (59.8% identity in 97 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE family protein PE20 2048371 PE20 885537 PE20 Mycobacterium tuberculosis H37Rv PE family protein PE20 YP_177843.1 2048072 D 83332 CDS YP_177653.1 57116920 885072 <2048398..2049597 1 NC_000962.3 Rv1807, (MTV049.29), len: 399 aa. PPE31, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1789|MTV049.11|AL022021 (393 aa), FASTA scores: opt: 1169, E(): 0, (49.5% identity in 412 aa overlap).; PPE family protein PPE31 2049597 PPE31 885072 PPE31 Mycobacterium tuberculosis H37Rv PPE family protein PPE31 YP_177653.1 <2048398 D 83332 CDS YP_177844.1 57116921 885590 2049921..2051150 1 NC_000962.3 Rv1808, (MTV049.30), len: 409 aa. PPE32, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1800|MTV049.22|AL022021 (655 aa), FASTA scores: opt: 1225, E(): 0, (55.1% identity in 423 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2050913 in the genome sequence has been corrected,A:G resulting in E331E.; PPE family protein PPE32 2051150 PPE32 885590 PPE32 Mycobacterium tuberculosis H37Rv PPE family protein PPE32 YP_177844.1 2049921 D 83332 CDS YP_177845.1 57116922 885555 2051282..2052688 1 NC_000962.3 Rv1809, (MTV049.31), len: 468 aa. PPE33, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to RV1802AL022021|MTV049.23 (463 aa), FASTA scores: opt: 1238, E(): 0, (51.2% identity in 471 aa overlap). Alternative nucleotide at position 2051746 (T->C; A155A) has been observed.; PPE family protein PPE33 2052688 PPE33 885555 PPE33 Mycobacterium tuberculosis H37Rv PPE family protein PPE33 YP_177845.1 2051282 D 83332 CDS NP_216326.1 15608947 885591 2052933..2053289 1 NC_000962.3 Rv1810, (MTCY16F9.04c), len: 118 aa. Conserved protein, similar to several hypothetical Mycobacterium tuberculosis proteins that may be exported (possible N-terminal signal sequence) e.g. O53953|Rv1804c|MTV049.26c|AL022021 (108 aa), FASTA scores: opt: 361, E(): 9.6e-17, (53.5% identity in 101 aa overlap); Rv0622, and Rv1690, etc.; hypothetical protein 2053289 885591 Rv1810 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216326.1 2052933 D 83332 CDS NP_216327.1 15608948 885439 2053443..2054147 1 NC_000962.3 Rv1811, (MTCY16F9.03c), len: 234 aa. Possible mgtC,magnesium (Mg2+) transport P-type ATPase C (transmembrane protein), highly similar to many e.g. NP_442124.1|NC_000911 Mg2+ transport ATPase from Synechocystis sp. strain PCC 6803 (234 aa); NP_251248.1|NC_002516 probable transport protein from Pseudomonas aeruginosa (230 aa); P22037|ATMC_SALTY|STM3764 magnesium transport ATPase protein C from Salmonella typhimurium (231 aa), FASTA scores: opt: 545, E(): 4.1e-30, (42.3% identity in 220 aa overlap); N-terminus of NP_213315.1|NC_000918 Mg(2+) transport ATPase from Aquifex aeolicus (225 aa); etc. Belongs to the MGTC / SAPB family; Possible Mg2+ transport P-type ATPase C MgtC 2054147 mgtC 885439 mgtC Mycobacterium tuberculosis H37Rv Possible Mg2+ transport P-type ATPase C MgtC NP_216327.1 2053443 D 83332 CDS NP_216328.1 15608949 885487 complement(2054157..2055359) 1 NC_000962.3 Rv1812c, (MTCY16F9.02), len: 400 aa. Probable dehydrogenase, similar to other dehydrogenases/oxidases e.g. AE001947|AE001947_10 NADH dehydrogenase II of Deinococcus radiodurans (379 aa), FASTA scores: opt: 404,E(): 3.4e-18, (26.4% identity in 363 aa overlap) and DHNA_HAEIN|P44856 nadh dehydrogenase (444 aa), FASTA scores: opt: 200, E(): 8.5e-06, (23.3% identity in 258 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical dehydrogenases Rv0392c, and Rv1854c|MTCY359.19 ndh probable NADH dehydrogenase (31.5% identity in 321 aa overlap).; Probable dehydrogenase 2055359 885487 Rv1812c Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_216328.1 2054157 R 83332 CDS NP_216329.1 15608950 885546 complement(2055681..2056112) 1 NC_000962.3 Rv1813c, (MTCY16F9.01), len: 143 aa. Conserved hypothetical protein. Possibly a exported protein with potential N-terminal signal sequence. Similar to Q11050|Rv1269c|MTCY50.13 hypothetical protein from Mycobacterium tuberculosis (124 aa), (42.7% identity in 143 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). Predicted possible vaccine candidate (See Zvi et al., 2008).; hypothetical protein 2056112 885546 Rv1813c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216329.1 2055681 R 83332 CDS NP_216330.1 15608951 885880 2056521..2057423 1 NC_000962.3 Rv1814, (MTCY1A11.29c), len: 300 aa. Erg3,transmembrane C-5 sterol desaturase (see *), weak similarity to several e.g. ERG3_YEAST|P32353 c-5 sterol desaturase (365 aa), FASTA scores: opt: 154, E(): 0.0011,(22.9% identity in 288 aa overlap). Belongs to the sterol desaturase family. [* Note: work of Jackson, C.J., Lamb,D.C., Kelly, D.E., Kelly, S.L., Characterization of a sterol delta 5,6-desaturase homolog in Mycobacterium bovis (BCG). Submitted (jun-2000) to the EMBL/GenBank/DDBJ databases].; Membrane-bound C-5 sterol desaturase Erg3 (sterol-C5-desaturase) 2057423 erg3 885880 erg3 Mycobacterium tuberculosis H37Rv Membrane-bound C-5 sterol desaturase Erg3 (sterol-C5-desaturase) NP_216330.1 2056521 D 83332 CDS NP_216331.1 15608952 885430 2057528..2058193 1 NC_000962.3 Rv1815, (MTCY1A11.28c), len: 221 aa. Conserved protein, similar to G473456 hypothetical protein from Mycobacterium fortuitum (255 aa), FASTA scores: opt: 182,E(): 3.2e-05, (29.6% identity in 230 aa overlap). Alternative nucleotide at position 2057774 (a->T; I83F) has been observed. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 2058193 885430 Rv1815 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216331.1 2057528 D 83332 CDS NP_216332.1 15608953 885340 2058256..2058960 1 NC_000962.3 Rv1816, (MTCY1A11.27c), len: 234 aa. Possible transcriptional regulatory protein. MEME analysis suggests similarity to putative Mycobacterium tuberculosis transcriptional regulators, Rv0653c, Rv0681. Contains helix-turn-helix motif at aa 38-59 (+4.30 SD).; Possible transcriptional regulatory protein 2058960 885340 Rv1816 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_216332.1 2058256 D 83332 CDS NP_216333.1 15608954 885548 2059595..2061058 1 NC_000962.3 Rv1817, (MTCY1A11.26c), len: 487 aa. Possible flavoprotein, similar to G746486 flavoprotein subunit of fumarate reductase FAD domain homologue (474 aa), FASTA scores: opt: 223, E(): 5.7e-07, (24.1% identity in 489 aa overlap); and AJ236923|SFR236923_3 soluble fumarate reductase of Shewanella frigidimarina ifcA (588 aa), FASTA scores: opt: 310, E(): 2.5e-11, (27.3% identity in 484 aa overlap).; Possible flavoprotein 2061058 885548 Rv1817 Mycobacterium tuberculosis H37Rv Possible flavoprotein NP_216333.1 2059595 D 83332 CDS YP_177846.1 57116923 885551 complement(2061178..2062674) 1 NC_000962.3 Rv1818c, (MTCY1A11.25), len: 498 aa. PE_PGRS33,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, similar to many. Contains 2 x PS00583 pfkB family of carbohydrate kinases signature 1. Supposedly localised to the cell surface (see citations below).; PE-PGRS family protein PE_PGRS33 2062674 PE_PGRS33 885551 PE_PGRS33 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS33 YP_177846.1 2061178 R 83332 CDS NP_216335.1 15608956 885539 complement(2062809..2064728) 1 NC_000962.3 Rv1819c, (MTCY1A11.24), len: 639 aa. Probable bacA,drug-transport transmembrane ATP-binding protein ABC transporter (see citation below), equivalent to AL008609|MLCB1788.47 hypothetical ABC transporter from Mycobacterium leprae (638 aa), (74.9% identity in 634 aa overlap). Also similar to other transmembrane ATP-binding proteins e.g. Q57335|Y036_HAEIN hypothetical ABC transporter ATP-binding protein from Haemophilus influenzae (592 aa), FASTA scores: opt: 1235, E(): 2.8e-61, (40.8% identity in 623 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable drug-transport transmembrane ATP-binding protein ABC transporter BacA 2064728 bacA 885539 bacA Mycobacterium tuberculosis H37Rv Probable drug-transport transmembrane ATP-binding protein ABC transporter BacA NP_216335.1 2062809 R 83332 CDS NP_216336.1 15608957 885738 2064799..2066442 1 NC_000962.3 Rv1820, (MTCY1A11.23c), len: 547 aa. Probable ilvG,acetolactate synthase. Equivalent to AL008609|MLCB1788.46c ilvG from Mycobacterium leprae (548 aa) (86.1% identity in 548 aa overlap). Similar to ILVB_KLEPN|P27696 (559 aa),FASTA scores: opt: 660, E(): 2.9e-34, (29.1% identity in 549 aa overlap). Also similar to other Mycobacterium tuberculosis Ilv proteins e.g. Rv3003c (ilvB), etc. Contains PS00187 Thiamine pyrophosphate enzymes signature.; Probable acetolactate synthase IlvG (acetohydroxy-acid synthase)(ALS) 2066442 ilvG 885738 ilvG Mycobacterium tuberculosis H37Rv Probable acetolactate synthase IlvG (acetohydroxy-acid synthase)(ALS) NP_216336.1 2064799 D 83332 CDS NP_216337.1 15608958 885594 2066457..2068883 1 NC_000962.3 Rv1821, (MTCY1A11.22c), len: 808 aa. Possible secA2,preprotein translocase and ATPase, component of secretion apparatus (see Braunstein & Belisle 2000), similar to several preprotein translocases e.g. P28366|SECA_BACSU preprotein translocase secA subunit from Bacillus subtilis (841 aa), FASTA scores: opt: 1424, E(): 0, (35.9% identity in 786 aa overlap). Equivalent to AL008609|MLCB1788.45 Preprotein translocase SecA 2 from Mycobacterium leprae (778 aa) (87.1% identity in 780 aa overlap). Also similar to Rv3240c|MTCY20B11.15c secA preprotein translocase from Mycobacterium tuberculosis (949 aa). Could be part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c,SECG|Rv1440 and SECY|Rv0732. Binds ATP.; Possible preprotein translocase ATPase SecA2 2068883 secA2 885594 secA2 Mycobacterium tuberculosis H37Rv Possible preprotein translocase ATPase SecA2 NP_216337.1 2066457 D 83332 CDS NP_216338.1 15608959 885126 2069080..2069709 1 NC_000962.3 Rv1822, (MTCY1A11.21c), len: 209 aa. Probable pgsA2,CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase (see citation below), integral membrane protein, equivalent to AL008609|MLCB1788_17 phosphatidyltransferase from Mycobacterium leprae (206 aa),FASTA score: (76.6% identity in 205 aa overlap). Also highly similar or similar to others e.g. CAB88885.1|AL353861 putative CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase from Streptomyces coelicolor (215 aa); AAC44003.1|U29587 phosphatidylglycerol phosphate synthase from Rhodobacter sphaeroides (227 aa); NP_405431.1|NC_003143 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Yersinia pestis (182 aa); P06978|PGSA_ECOLI CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Escherichia coli (181 aa),FASTA scores: opt: 252, E(): 2.8e-09, (29.7% identity in 175 aa overlap); etc. Also similar to Rv2746c|PGSA3|MTV002.11c CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (PGP synthase) from Mycobacterium tuberculosis (209 aa). Contains PS00379 CDP-alcohol phosphatidyltransferases signature; and PS00075 Dihydrofolate reductase signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family.; Probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA2 (PGP synthase) (phosphatidylglycerophosphate synthase) (3-phosphatidyl-1'-glycerol-3'phosphate synthase) 2069709 pgsA2 885126 pgsA2 Mycobacterium tuberculosis H37Rv Probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA2 (PGP synthase) (phosphatidylglycerophosphate synthase) (3-phosphatidyl-1'-glycerol-3'phosphate synthase) NP_216338.1 2069080 D 83332 CDS NP_216339.1 15608960 885547 2069702..2070625 1 NC_000962.3 Rv1823, (MTCY01A11.20), len: 307 aa. Conserved protein, similar to P71582|MTCY10H4.12|RV0012 hypothetical protein CY10H4.12 from Mycobacterium tuberculosis (262 aa),FASTA scores: opt: 304, E(): 1.5e-12, (30.1% identity in 246 aa overlap).; hypothetical protein 2070625 885547 Rv1823 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216339.1 2069702 D 83332 CDS NP_216340.1 15608961 885719 2070654..2071019 1 NC_000962.3 Rv1824, (MTCY1A11.19c), len: 121 aa. Conserved hypothetical membrane protein similar to P28265|SBP_BACSU sbp protein from Bacillus subtilis (121 aa), FASTA scores: opt: 261, E(): 1.9e-12, (38.9% identity in 113 aa overlap).; hypothetical protein 2071019 885719 Rv1824 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216340.1 2070654 D 83332 CDS NP_216341.1 15608962 885726 2071036..2071914 1 NC_000962.3 Rv1825, (MTCY1A11.18c), len: 292 aa. Conserved protein, weak similarity to Mycobacterium tuberculosis hypothetical proteins Q50610|MTCY1A11.20C|Rv1823|Z78020 (307 aa), FASTA scores: opt: 182, E(): 0.00044, (29.9% identity in 204 aa overlap); and Rv0012. Has a hydrophobic stretch, TMhelix from aa 67 to 85.; hypothetical protein 2071914 885726 Rv1825 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216341.1 2071036 D 83332 CDS NP_216342.1 15608963 885720 2071952..2072356 1 NC_000962.3 Rv1826, (MTCY1A11.17c), len: 134 aa. Probable gcvH,glycine cleavage system H protein, highly similar to GCSH_ECOLI|P23884 glycine cleavage system H protein from Escherichia coli (129 aa), FASTA scores: opt: 428, E(): 2.2e-22, (47.8% identity in 134 aa overlap). Equivalent to MLCB1788.37c gcvH from Mycobacterium leprae (78.4% identity in 134 aa overlap). Contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. Belongs to the GcvH family.; Probable glycine cleavage system H protein GcvH 2072356 gcvH 885720 gcvH Mycobacterium tuberculosis H37Rv Probable glycine cleavage system H protein GcvH NP_216342.1 2071952 D 83332 CDS NP_216343.1 15608964 885735 2072596..2073084 1 NC_000962.3 Rv1827, (MTCY1A11.16c), len: 162 aa. GarA, conserved protein with forkhead-associated domain at C-terminus (see citation below), equivalent to O32919|MLCB1788.36c hypothetical protein from Mycobacterium leprae (162 aa),FASTA scores: opt: 888, E(): 0, (87.0% identity in 161 aa overlap). Putative physiological substrate of PknB and PknG.; Conserved protein with FHA domain, GarA 2073084 garA 885735 garA Mycobacterium tuberculosis H37Rv Conserved protein with FHA domain, GarA NP_216343.1 2072596 D 83332 CDS NP_216344.1 15608965 885336 2073081..2073824 1 NC_000962.3 Rv1828, (MTCY1A11.15c), len: 247 aa. Conserved protein, equivalent to O32918|MLCB1788.35c|AL008609 hypothetical protein from Mycobacterium leprae (251 aa),FASTA scores: opt: 1397, E(): 0, (87.6% identity in 251 aa overlap).; hypothetical protein 2073824 885336 Rv1828 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216344.1 2073081 D 83332 CDS NP_216345.1 15608966 885743 2073943..2074437 1 NC_000962.3 Rv1829, (MTCY1A11.14c), len: 164 aa. Conserved protein, equivalent to O32917|MLCB1788.34|AL008609 Hypothetical protein from Mycobacterium leprae (164 aa),FASTA scores: opt: 1011, E(): 0, (95.1% identity in 164 aa overlap). Also present in Aquifex aeolicus, etc.; hypothetical protein 2074437 885743 Rv1829 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216345.1 2073943 D 83332 CDS NP_216346.1 15608967 885740 2074841..2075518 1 NC_000962.3 Rv1830, (MTCY1A11.13c), len: 225 aa. Conserved hypothetical protein, equivalent to Mycobacterium leprae hypothetical protein MLCB1788.33c|AL008609|O32916 (231 aa),FASTA scores: opt: 1307, E(): 0, (89.6% identity in 231 aa overlap).; hypothetical protein 2075518 885740 Rv1830 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216346.1 2074841 D 83332 CDS NP_216347.1 15608968 885718 2075571..2075828 1 NC_000962.3 Rv1831, (MTCY1A11.12c), len: 85 aa. Hypothetical unknown protein.; Hypothetical protein 2075828 885718 Rv1831 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216347.1 2075571 D 83332 CDS NP_216348.1 15608969 885716 2075877..2078702 1 NC_000962.3 Rv1832, (MTCY1A11.11c), len: 941 aa. Probable gcvB,glycine dehydrogenase [decarboxylating], highly similar to GCSP_ECOLI|P33195 glycine dehydrogenase (decarboxylating) from Escherichia coli (957 aa), FASTA scores: opt: 2194,E(): 0, (55.4% identity in 961 aa overlap). The glycine cleavage system is composed of four proteins: P, T, L, and H; Probable glycine dehydrogenase GcvB (glycine decarboxylase) (glycine cleavage system P-protein) 2078702 gcvB 885716 gcvB Mycobacterium tuberculosis H37Rv Probable glycine dehydrogenase GcvB (glycine decarboxylase) (glycine cleavage system P-protein) NP_216348.1 2075877 D 83332 CDS NP_216349.1 15608970 885737 complement(2078929..2079789) 1 NC_000962.3 Rv1833c, (MTCY1A11.10), len: 286 aa. Possible haloalkane dehalogenase. Similar to several haloalkane dehalogenase e.g. CAB45532.1|AJ243259 from Mycobacterium bovis (300 aa); also similar to LINB_PSEPA|P51698 1,3,4,6-tetrachloro-1,4-cyclohexadien from Pseudomonas paucimobilis (295 aa), FASTA scores: opt: 314, E(): 1.5e-13, (33.1% identity in 281 aa overlap).; Possible haloalkane dehalogenase 2079789 885737 Rv1833c Mycobacterium tuberculosis H37Rv Possible haloalkane dehalogenase NP_216349.1 2078929 R 83332 CDS NP_216350.1 15608971 888761 2079830..2080696 1 NC_000962.3 Rv1834, (MTCY1A11.09c), len: 288 aa. Probable lipZ,hydrolase, some similarity to haloalkane dehalogenases and D16262 hypothetical 38.9 kDa protein (335 aa), FASTA scores: opt: 507, E(): 7.6e-28, (33.0% identity in 300 aa overlap).; Probable hydrolase 2080696 lipZ 888761 lipZ Mycobacterium tuberculosis H37Rv Probable hydrolase NP_216350.1 2079830 D 83332 CDS NP_216351.1 15608972 885877 complement(2080701..2082587) 1 NC_000962.3 Rv1835c, (MTCY1A11.08), len: 628 aa. Conserved hypothetical protein, some similarity to putative acylases e.g. G216374 glutaryl 7-aca acylase precursor (634 aa) FASTA scores, opt: 202, E(): 3.5e-06, (25.1% identity in 669 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv2800 and Rv1215c.; hypothetical protein 2082587 885877 Rv1835c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216351.1 2080701 R 83332 CDS NP_216352.1 15608973 885707 complement(2082603..2084636) 1 NC_000962.3 Rv1836c, (MTCY1A11.07), len: 677 aa. Conserved protein. Equivalent to MLCB1788.28|AL008609 hypothetical protein from Mycobacterium leprae (710 aa), FASTA scores: opt: 2938, E(): 0, (66.0% identity in 714 aa overlap). Contains PS00036 bZIP transcription factors basic domain signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2084636 885707 Rv1836c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216352.1 2082603 R 83332 CDS NP_216353.1 15608974 885713 complement(2084756..2086981) 1 NC_000962.3 Rv1837c, (MTCY1A11.06), len: 741 aa. glcB, malate synthase G (see citations below), highly similar to MASY_CORGL|P42450 malate synthase (738 aa), FASTA score: opt: 2961, E(): 0, (61.3% identity in 724 aa overlap). Belongs to the malate synthase G family.; Malate synthase G GlcB 2086981 glcB 885713 glcB Mycobacterium tuberculosis H37Rv Malate synthase G GlcB NP_216353.1 2084756 R 83332 CDS NP_216354.1 15608975 885744 complement(2087257..2087652) 1 NC_000962.3 Rv1838c, (MTCY359.35), len: 131 aa. Possible vapC13,toxin, part of toxin-antitoxin (TA) operon with Rv1839c,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Part of 14-membered Mycobacterium tuberculosis protein family with Rv2863|MTV003.09|AL008883 (126 aa), FASTA scores: opt: 293, E(): 1.5e-14, (38.2% identity in 123 aa overlap); Rv0749, Rv0277c, Rv2530c, etc. Also similar to AJ248288|CNSPAX06_181 Pyrococcus abyssi complete genome (136 aa), FASTA scores: opt: 197, E(): 2.2e-07, (33. 1% identity in 133 aa overlap).; Possible toxin VapC13 2087652 vapC13 885744 vapC13 Mycobacterium tuberculosis H37Rv Possible toxin VapC13 NP_216354.1 2087257 R 83332 CDS NP_216355.1 15608976 885750 complement(2087649..2087912) 1 NC_000962.3 Rv1839c, (MTCY359.34), len: 87 aa. Possible vapB13,antitoxin, part of toxin-antitoxin (TA) operon with Rv1838c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to others in M. tuberculosis e.g. Rv0239,Rv0662c; Possible antitoxin VapB13 2087912 vapB13 885750 vapB13 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB13 NP_216355.1 2087649 R 83332 CDS YP_177847.1 57116924 885753 complement(2087971..2089518) 1 NC_000962.3 Rv1840c, (MTCY359.33), len: 515 aa. PE_PGRS34,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to many e.g. Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), FASTA scores: opt: 1693, E(): 0, (53.1% identity in 612 aa overlap); etc.; PE-PGRS family protein PE_PGRS34 2089518 PE_PGRS34 885753 PE_PGRS34 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS34 YP_177847.1 2087971 R 83332 CDS NP_216357.1 15608978 885656 complement(2089681..2090718) 1 NC_000962.3 Rv1841c, (MTCY359.32), len: 345 aa. Conserved hypothetical membrane protein. Some similarity to O07585|YHDP_BACSU hypothetical 49.9 kDa protein from Bacillus subtilis (444 aa), FASTA scores: opt: 620, E(): 0,(31.1% identity in 350 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. Rv1842c, Rv2366c.; hypothetical protein 2090718 885656 Rv1841c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216357.1 2089681 R 83332 CDS NP_216358.1 15608979 885739 complement(2090718..2092085) 1 NC_000962.3 Rv1842c, (MTCY359.31), len: 455 aa. Conserved hypothetical membrane protein. Similar to Z99109|0O7589 Potential integral membrane protein from Bacillus subtilis (461 aa), FASTA scores: opt: 723, E(): 0, (31.2% identity in 449 aa overlap). Similar to other Mycobacterium tuberculosis putative integral membrane proteins e.g. Rv2366c, Rv1841c.; hypothetical protein 2092085 885739 Rv1842c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216358.1 2090718 R 83332 CDS NP_216359.1 15608980 885714 complement(2092259..2093698) 1 NC_000962.3 Rv1843c, (MTCY359.30), len: 479 aa. Probable guaB1,inosine-5'-monophosphate dehydrogenase. Similar to others e.g. IMDH_BACSU|P21879 from Bacillus subtilis (513 aa),FASTA score: opt: 904, E(): 0, (37.8% identity in 471 aa overlap). Similar to other Mycobacterium tuberculosis proteins e.g. guaB2, Rv3411c.; Probable inosine-5'-monophosphate dehydrogenase GuaB1(imp dehydrogenase) (IMPDH) (IMPD) 2093698 guaB1 885714 guaB1 Mycobacterium tuberculosis H37Rv Probable inosine-5'-monophosphate dehydrogenase GuaB1(imp dehydrogenase) (IMPDH) (IMPD) NP_216359.1 2092259 R 83332 CDS YP_177848.1 57116925 885755 complement(2093731..2095188) 1 NC_000962.3 Rv1844c, (MTCY359.29), len: 485 aa. Probable gnd1,6-phosphogluconate dehydrogenase. Similar to others e.g. 6PGD_ECOLI|P00350 from Escherichia coli (468 aa), FASTA scores: opt: 1661, E(): 0, (53.6% identity in 466 aa overlap); etc. Also similar to Rv1122|MTCY22G8.11|gnd2 probable 6-phosphogluconate dehydrogenase, decarboxylating from Mycobacterium tuberculosis (340 aa), FASTA score: (33.0% identity in 351 aa overlap). Note that Rv1844c is most similar to gnd's from Gram negative organisms, while Rv1122|MTCY22G8.11|gnd2 is most similar to gnd's from Gram positive organisms. Belongs to the 6-phosphogluconate dehydrogenase family.; Probable 6-phosphogluconate dehydrogenase Gnd1 2095188 gnd1 885755 gnd1 Mycobacterium tuberculosis H37Rv Probable 6-phosphogluconate dehydrogenase Gnd1 YP_177848.1 2093731 R 83332 CDS NP_216361.1 15608982 885370 complement(2095218..2096168) 1 NC_000962.3 Rv1845c, (MTCY359.28), len: 316 aa. Possible blaR,sensor-transducer protein. Conserved hypothetical transmembrane protein. Equivalent to MLCB1788.18|AL008609 Hypothetical protein from Mycobacterium leprae (316 aa),FASTA scores: opt: 1762, E(): 0, (87.6% identity in 314 aa overlap). Similar to proteins in Streptomyces coelicolor e.g. SC10A7.04|AL078618.1.; Possible sensor-transducer protein BlaR 2096168 blaR 885370 blaR Mycobacterium tuberculosis H37Rv Possible sensor-transducer protein BlaR NP_216361.1 2095218 R 83332 CDS NP_216362.1 15608983 885747 complement(2096183..2096599) 1 NC_000962.3 Rv1846c, (MTCY359.27), len: 138 aa. BlaI,transcriptional repressor. Equivalent to MLCB1788.17|AL008609 hypothetical protein from Mycobacterium leprae (142 aa), FASTA scores: opt: 736 E(): 0, (95.1% identity in 123 aa overlap). Also similar to BLAI_BACLI|P06555 penicillinase repressor (128 aa), fasta scores: opt: 114, E(): 0.12, (23.7% identity in 131 aa overlap).; Transcriptional repressor BlaI 2096599 blaI 885747 blaI Mycobacterium tuberculosis H37Rv Transcriptional repressor BlaI NP_216362.1 2096183 R 83332 CDS NP_216363.1 15608984 885734 2096877..2097299 1 NC_000962.3 Rv1847, (MTCY359.26c), len: 140 aa. Conserved protein, possible thioesterase, some similarity to YBDB proteins of Escherichia coli and H. influenzae e.g. P15050|YBDB_ECOLI hypothetical 15.0 KD protein in ENTA-CSTA intergenic region (137 aa), FASTA scores: opt: 232, E(): 6.6e-10, (35.8% identity in 106 aa overlap); C48956|G142208 thioesterase from Arthrobacter sp (151 aa), FASTA score: opt: 254, E(): 1.7e-11, (33.3% identity in 138 aa overlap). Also similar to AF064959|AF064959_1 hypothetical protein from Coxiella burnetii (148 aa), FASTA score: opt: 264,E(): 9.3e- 12, (36.8% identity in 117 aa overlap).; hypothetical protein 2097299 885734 Rv1847 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216363.1 2096877 D 83332 CDS NP_216364.1 15608985 885414 2097348..2097650 1 NC_000962.3 Rv1848, (MTCY359.25c), len: 100 aa. UreA, urease gamma subunit. Similar to URE3_MYCTU|P50043 from Mycobacterium tuberculosis (100 aa), FASTA scores: opt: 630, E(): 1.3e-36, (99.0% identity in 100 aa overlap). Belongs to the urease gamma subunit family.; Urease gamma subunit UreA (urea amidohydrolase) 2097650 ureA 885414 ureA Mycobacterium tuberculosis H37Rv Urease gamma subunit UreA (urea amidohydrolase) NP_216364.1 2097348 D 83332 CDS NP_216365.1 15608986 885710 2097647..2097961 1 NC_000962.3 Rv1849, (MTCY359.24c), len: 104 aa. UreB, urease beta subunit. Identical to URE2_MYCTU|P50048 urease beta subunit from Mycobacterium tuberculosis (100 aa). Belongs to the urease gamma subunit family.; Urease beta subunit UreB (urea amidohydrolase) 2097961 ureB 885710 ureB Mycobacterium tuberculosis H37Rv Urease beta subunit UreB (urea amidohydrolase) NP_216365.1 2097647 D 83332 CDS NP_216366.1 15608987 885359 2097961..2099694 1 NC_000962.3 Rv1850, (MTCY359.23c), len: 577 aa. UreC, urease alpha subunit. Similar to URE1_MYCTU|P50042 from Mycobacterium tuberculosis (577 aa), FASTA scores: opt: 3794, E(): 0, (98.3% identity in 577 aa overlap). Contains PS00145 Urease active site motif. Belongs to the urease family.; Urease alpha subunit UreC (urea amidohydrolase) 2099694 ureC 885359 ureC Mycobacterium tuberculosis H37Rv Urease alpha subunit UreC (urea amidohydrolase) NP_216366.1 2097961 D 83332 CDS NP_216367.1 15608988 885532 2099694..2100329 1 NC_000962.3 Rv1851, (MTCY359.22c), len: 211 aa. UreF, urease accessory protein. Identical to UREF_MYCTU|P50050 from M. tuberculosis.; Urease accessory protein UreF 2100329 ureF 885532 ureF Mycobacterium tuberculosis H37Rv Urease accessory protein UreF NP_216367.1 2099694 D 83332 CDS NP_216368.1 15608989 885729 2100340..2101014 1 NC_000962.3 Rv1852, (MTCY359.21c), len: 224 aa. UreG, urease accessory protein. Identical to UREG_MYCTU|P50051 from M. tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UreG family.; Urease accessory protein UreG 2101014 ureG 885729 ureG Mycobacterium tuberculosis H37Rv Urease accessory protein UreG NP_216368.1 2100340 D 83332 CDS NP_216369.1 15608990 885705 2101022..2101648 1 NC_000962.3 Rv1853, (MTCY359.20c), len: 208 aa. UreD, probable urease accessory protein. Similar to URED_YEREN|P42868 Urease operon ureD protein from Yersinia enterocolitica (325 aa), Fasta scores: opt: 114, E(): 0.37, (25.2% identity in 119 aa overlap).; Probable urease accessory protein UreD 2101648 ureD 885705 ureD Mycobacterium tuberculosis H37Rv Probable urease accessory protein UreD NP_216369.1 2101022 D 83332 CDS NP_216370.1 15608991 885746 complement(2101651..2103042) 1 NC_000962.3 Rv1854c, (MTCY359.19), len: 463 aa. Probable ndh,NADH dehydrogenase (see citations below), similar to several e.g. S74826 NADH dehydrogenase from Synechocystis sp. (445 aa), FASTA score: opt: 1228, E(): 0, (46.3% identity in 432 aa overlap). Highly similar to Rv0392c|Z84725|g1817703 from Mycobacterium tuberculosis (470 aa), FASTA scores: opt: 1911, E(): 0, (64.7% identity in 459 aa overlap); and Rv1812c.; Probable NADH dehydrogenase Ndh 2103042 ndh 885746 ndh Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase Ndh NP_216370.1 2101651 R 83332 CDS NP_216371.1 15608992 885736 complement(2103184..2104107) 1 NC_000962.3 Rv1855c, (MTCY359.18), len: 307 aa. Possible oxidoreductase, possibly a monooxygenase. Contains PS00217 Sugar transport proteins signature 2, probably fortuitously. Similar to G487716 (78-11) lincomycin production genes (29.2% identity in 154 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. Rv0953c, Rv0791c, Rv0132c, Rv2951c, etc.; Possible oxidoreductase 2104107 885736 Rv1855c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_216371.1 2103184 R 83332 CDS NP_216372.1 15608993 885708 complement(2104146..2104823) 1 NC_000962.3 Rv1856c, (MTCY359.17), len: 225 aa. Possible oxidoreductase. Equivalent to MLCB1788.11c|AL008609 oxidoreductase from Mycobacterium leprae (224 aa), FASTA scores: opt: 1211, E(): 0; (80.4% identity in 224 aa overlap). Some similarity to dehydrogenases of short-chain dehydrogenase/reductase family and fatty-acyl CoA reductases e.g. P16543|DHK2_STRVN granaticin polyketide synthase P (249 aa), FASTA score: opt: 194, E(): 1.1e-05,(32.5% identity in 237 aa overlap).; Possible oxidoreductase 2104823 885708 Rv1856c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_216372.1 2104146 R 83332 CDS NP_216373.1 15608994 885655 2104985..2105770 1 NC_000962.3 Rv1857, (MTCY359.16c), len: 261 aa. Probable modA,molybdate-binding protein attached to membrane by lipid-modified N-terminal cysteine (contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site),component of molybdate transport system (see citations below). Shows strong similarity to precursors of periplasmic molybdate/sulphate binding proteins e.g. O31229|Y10817|ANY108174 ModA from Arthrobacter nicotinovorans (260 aa), FASTA score: opt: 725, E(): 0,(47.8% identity in 249 aa overlap).; Probable molybdate-binding lipoprotein ModA 2105770 modA 885655 modA Mycobacterium tuberculosis H37Rv Probable molybdate-binding lipoprotein ModA NP_216373.1 2104985 D 83332 CDS NP_216374.1 15608995 885723 2105773..2106567 1 NC_000962.3 Rv1858, (MTCY359.15c), len: 264 aa. Probable modB,molybdenum-transport integral membrane protein ABC transporter (see citation below), similar to others e.g. Y10817|ANY108175 ModB from Arthrobacter (239 aa), FASTA scores: opt: 937, E(): 0, (67.8% identity in 230 aa overlap); etc. Similar to other Mycobacterium tuberculosis transport proteins e.g. Rv2039c, Rv2316, etc.; Probable molybdenum-transport integral membrane protein ABC transporter ModB 2106567 modB 885723 modB Mycobacterium tuberculosis H37Rv Probable molybdenum-transport integral membrane protein ABC transporter ModB NP_216374.1 2105773 D 83332 CDS NP_216375.1 15608996 885731 2106574..2107683 1 NC_000962.3 Rv1859, (MTCY359.14c), len: 369 aa. Probable modC,molybdenum-transport ATP-binding protein ABC transporter (see citation below), similar to others e.g. Y10817|ANY108176 ModC from Arthrobacter (349 aa), FASTA scores: opt: 895, E(): 0, (46.0% identity in 361 aa overlap); etc. Shows similarity to other Mycobacterium tuberculosis ABC-transporter proteins e.g. Rv0073, Rv1238,Rv2564, etc. Contains both PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signatures involved in molybdate uptake. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable molybdenum-transport ATP-binding protein ABC transporter ModC 2107683 modC 885731 modC Mycobacterium tuberculosis H37Rv Probable molybdenum-transport ATP-binding protein ABC transporter ModC NP_216375.1 2106574 D 83332 CDS YP_177849.1 57116926 885896 2107736..2108713 1 NC_000962.3 Rv1860, (MT1908, MTCY359.0013), len: 325 aa. Apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). Changes in the mannosylation pattern of this protein affect its ability to stimulate T-lymphocyte response. Major immunodominant antigen that has potential as a vaccine against tuberculosis. APA-ELISA could be used in diagnosis.; Alanine and proline rich secreted protein Apa (fibronectin attachment protein) (immunogenic protein MPT32) (antigen MPT-32) (45-kDa glycoprotein) (45/47 kDa antigen) 2108713 apa 885896 apa Mycobacterium tuberculosis H37Rv Alanine and proline rich secreted protein Apa (fibronectin attachment protein) (immunogenic protein MPT32) (antigen MPT-32) (45-kDa glycoprotein) (45/47 kDa antigen) YP_177849.1 2107736 D 83332 CDS NP_216377.1 15608998 885741 2109165..2109470 1 NC_000962.3 Rv1861, (MTCY359.12c), len: 101 aa. Probable conserved transmembrane protein, showing weak similarity to AE002069|AE002069_10 hypothetical protein from Deinococcus radiodurans (146 aa), FASTA scores: opt: 154, E(): 0.0027,(30.8% identity in 104 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 2109470 885741 Rv1861 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216377.1 2109165 D 83332 CDS NP_216378.1 15608999 885652 2109544..2110584 1 NC_000962.3 Rv1862, (MTCY359.11), len: 346 aa. Probable adhA,alcohol dehydrogenase, similar to ADH2_BACST|P42327 alcohol dehydrogenase (339 aa), FASTA scores: opt: 630, E(): 2.4e-32 (34.4% identity in 320 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature.; Probable alcohol dehydrogenase AdhA 2110584 adhA 885652 adhA Mycobacterium tuberculosis H37Rv Probable alcohol dehydrogenase AdhA NP_216378.1 2109544 D 83332 CDS NP_216379.1 15609000 885722 complement(2110591..2111361) 1 NC_000962.3 Rv1863c, (MTCY359.10), len: 256 aa. Probable conserved integral membrane protein, similar to Rv0804|Z95618|MTCY7H7A.05 Hypothetical protein from Mycobacterium tuberculosis (209 aa), FASTA scores: opt: 199, E(): 1e-06, (33.2% identity in 220 aa overlap); and Rv0658c.; Probable conserved integral membrane protein 2111361 885722 Rv1863c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216379.1 2110591 R 83332 CDS NP_216380.1 15609001 885800 complement(2111354..2112109) 1 NC_000962.3 Rv1864c, (MTCY359.09), len: 251 aa. Conserved protein. Similar to other hypothetical proteins e.g. AL031317|SC6G4.43 from Streptomyces coelicolor cosmid 6G (233 aa), FASTA scores: opt: 716, E(): 0, (54.4% identity in 215 aa overlap); also P43976|YIIM_HAEIN hypothetical protein hi0278 (221 aa), FASTA scores: opt: 223, E(): 3.8e-08, (29.5% identity in 173 aa overlap).; hypothetical protein 2112109 885800 Rv1864c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216380.1 2111354 R 83332 CDS NP_216381.1 15609002 885806 complement(2112106..2112966) 1 NC_000962.3 Rv1865c, (MTCY359.08), len: 286 aa. Probable short-chain dehydrogenase, highly similar to C-terminus of NP_301650.1|NC_00267 putative oxidoreductase from Mycobacterium leprae (596 aa). Also similar to various dehydrogenases, generally belonging to short-chain family,e.g. AAG02168.1|AF212041_24|AF212041 3-oxoacyl-(acylcarrier protein) reductase from Zymomonas mobilis (251 aa); P50198|LINX_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-DIOL dehydrogenase from Sphingomonas paucimobilis (250 aa); NP_105680.1|NC_002678 sorbitol dehydrogenase (also similar to acetoin reductase) from Mesorhizobium loti (256 aa); etc. And highly similar to C-terminus of ephD|Rv2214c|MTCY190.25c from Mycobacterium tuberculosis (592 aa); and many other oxidoreductases from Mycobacterium tuberculosis e.g. Y00P_MYCTU|Q10402 putative oxidoreductase (650 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.5% identity in 280 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase 2112966 885806 Rv1865c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase NP_216381.1 2112106 R 83332 CDS NP_216382.3 448824765 885733 2113140..2115476 1 NC_000962.3 Rv1866, (MTCY359.07c), len: 778 aa. Conserved protein, N-terminal region similar to fatty acyl-CoA racemases e.g. Rv0855, Rv1143, and C-terminal region (from aa 370) similar to L-carnitine dehydratases, racemases, and Rv3272|MTCY71.12 Mycobacterium tuberculosis (394 aa), FASTA score: opt: 472, E(): 2.1e-21, (29.9% identity in 388 aa overlap). Also similar to P31572|CAIB_ECOLI L-carnitine dehydratase (405 aa), FASTA score: opt: 306, E(): 2.1e-11,(23.3% identity in 424 aa overlap).; hypothetical protein 2115476 885733 Rv1866 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216382.3 2113140 D 83332 CDS NP_216383.1 15609004 885704 2115764..2117248 1 NC_000962.3 Rv1867, (MTCY359.06c), len: 494 aa. Conserved protein, some similarity to acetyl CoA synthase and to lipid carriers. FASTA best: E155295 acetyl CoA synthase (388 aa), opt: 213, E(): 4.5e-07, (23.2% identity in 423 aa overlap); hypothetical protein 2117248 885704 Rv1867 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216383.1 2115764 D 83332 CDS NP_216384.1 15609005 885757 2117347..2119446 1 NC_000962.3 Rv1868, (MTCY359.05c), len: 699 aa. Conserved hypothetical protein, similar to products of three consecutive ORFS in Mycobacterium leprae MLCB2052.18|Z98604|B2052 (257 aa), FASTA scores: opt: 314,E(): 9.9e-12, (35.2% identity in 213 aa overlap); MLCB2052.17, and MLCB2052.16. Also similar to M. tuberculosis hypothetical protein Rv2047c.; hypothetical protein 2119446 885757 Rv1868 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216384.1 2117347 D 83332 CDS NP_216385.1 15609006 885796 complement(2119460..2120695) 1 NC_000962.3 Rv1869c, (MTCY359.04), len: 411 aa. Probable reductase (1.-.-.-). Similar to several reductases e.g. CAC04223.1|AL391515 putative ferredoxin reductase from Streptomyces coelicolor (420 aa); THCD_RHOSO|P43494 rhodocoxin reductase (426 aa), FASTA scores: opt: 904, E(): 0, (40.8% identity in 370 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv0688 (406 aa) (39.9% identity in 391 aa overlap); and Rv0253 (nitrite reductase subunit).; Probable reductase 2120695 885796 Rv1869c Mycobacterium tuberculosis H37Rv Probable reductase NP_216385.1 2119460 R 83332 CDS NP_216386.1 15609007 885797 complement(2120795..2121430) 1 NC_000962.3 Rv1870c, (MTCY359.03), len: 211 aa. Conserved hypothetical protein. Some similarity to SC6F7.17c hypothetical protein from Streptomyces coelicolor (216 aa).; hypothetical protein 2121430 885797 Rv1870c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216386.1 2120795 R 83332 CDS NP_216387.1 15609008 885804 complement(2121495..2121884) 1 NC_000962.3 Rv1871c, (MTCY359.02), len: 129 aa. Conserved protein, similar to Mycobacterium tuberculosis hypothetical proteins Q11057|Rv1261|MTCY50.21 (149 aa), FASTA score: opt: 125, E(): 0.019, (32.6% identity in 89 aa overlap); Rv0523c, and Rv1598c.; hypothetical protein 2121884 885804 Rv1871c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216387.1 2121495 R 83332 CDS NP_216388.1 15609009 885754 complement(2121907..2123151) 1 NC_000962.3 Rv1872c, (MTCY180.46, MTCY359.01), len: 414 aa (start uncertain). Possible lldD2, L-lactate dehydrogenase (cytochrome), similar to other lactate dehydrogenases and other oxidases e.g. LLDD_ECOLI|P33232 l-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA results: opt: 674, E(): 1.1e-37, (40.5% identity in 279 aa overlap); Q51135 lactate dehydrogenase from Neisseria meningitidis (390 aa), FASTA results: opt: 309, E(): 4.1e-15, (42.5% identity in 113 aa overlap); etc. Also shows similarity with Rv0694|lldD1|MTCY210.11 possible L-lactate dehydrogenase (cytochrome) from Mycobacterium tuberculosis (396 aa). Contains PS00557 FMN-dependent alpha-hydroxy acid dehydrogenases active site. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenases family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al.,2010).; Possible L-lactate dehydrogenase (cytochrome) LldD2 2123151 lldD2 885754 lldD2 Mycobacterium tuberculosis H37Rv Possible L-lactate dehydrogenase (cytochrome) LldD2 NP_216388.1 2121907 R 83332 CDS NP_216389.1 15609010 885789 2123174..2123611 1 NC_000962.3 Rv1873, (MTCY180.45c), len: 145 aa. Conserved hypothetical protein. Some similarity to AL591783 hypothetical protein from Sinorhizobium meliloti.; hypothetical protein 2123611 885789 Rv1873 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216389.1 2123174 D 83332 CDS NP_216390.3 448824766 885748 2123684..2124370 1 NC_000962.3 Rv1874, (MTCY180.44c), len: 228 aa. Unknown protein.; hypothetical protein 2124370 885748 Rv1874 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216390.3 2123684 D 83332 CDS NP_216391.1 15609012 885793 2124381..2124824 1 NC_000962.3 Rv1875, (MTCY180.43c), len: 147 aa. Conserved protein. Some similarity to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1155|MTCI65.22|Z95584 (147 aa), FASTA scores: opt: 178, E(): 7.4e-06, (26.9% identity in 130 aa overlap); Rv0121c and Rv2074. Also similar to AL079356|SC6G9.21 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 239, E(): 3.1 e-09,(38.7% identity in 137 aa overlap).; hypothetical protein 2124824 885793 Rv1875 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216391.1 2124381 D 83332 CDS NP_216392.1 15609013 885767 2125340..2125819 1 NC_000962.3 Rv1876, (MTCY180.42c), len: 159 aa. Probable bfrA (alternate gene name: bfr), bacterioferritin (see citation below), similar to BFR_MYCLE|P43315 bacterioferritin (bfr) from Mycobacterium leprae (159 aa), FASTA results: opt: 958, E(): 0, (90.6% identity in 159 aa overlap). Also similar to Rv3841|MTCY01A6.28c|bfrB possible bacterioferritin from Mycobacterium tuberculosis (181 aa). Belongs to the bacterioferritin family.; Probable bacterioferritin BfrA 2125819 bfrA 885767 bfrA Mycobacterium tuberculosis H37Rv Probable bacterioferritin BfrA NP_216392.1 2125340 D 83332 CDS NP_216393.1 15609014 885654 2125904..2127967 1 NC_000962.3 Rv1877, (MTCY180.41c), len: 687 aa. Probable conserved integral membrane protein, part of major facilitator superfamily (MFS), similar to many antibiotic and drug efflux proteins. Similar to e.g. Q56175 TU22 dTDP-glucose dehydrtatase from Streptomyces violaceoruber (557 aa), FASTA scores: opt: 895, E(): 0, (34.7% identity in 528 aa overlap). Also similar to Mycobacterium tuberculosis relatives protein, include Rv3728, Rv3239c,Rv2846c, etc. Contains PS00217 Sugar transport proteins signature 2 (PS00217).; Probable conserved integral membrane protein 2127967 885654 Rv1877 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216393.1 2125904 D 83332 CDS NP_216394.1 15609015 885761 2128022..2129374 1 NC_000962.3 Rv1878, (MTCY180.40c), len: 450 aa. Probable glnA3,glutamine synthetase class I, similar to many e.g. GLNA_BACCE|P19064 from Bacillus cereus (443 aa), FASTA results: opt: 497, E(): 5.2e-23, (29.0% identity in 331 aa overlap); etc. Also similar to C-terminus of FLUG_EMENI|P38094 flug protein from emericella nidulans (865 aa), FASTA scores: opt: 227, E (): 6.4e-13, (29.9% identity in 394 aa overlap). Note that the downstream ORF MTCY180.39c is similar to the N-terminus. Also similar to three other potential glutamine synthases in M. tuberculosis: Q10378|GLN2_MYCTU|GLNA2|Rv2222c|MT2280|MTCY190.33c|MTCY427 .03c; Rv2860c|MTV003.06c|glnA4 and Rv2220|glnA1. Belongs to the glutamine synthetase family.; Probable glutamine synthetase GlnA3 (glutamine synthase) (GS-I) 2129374 glnA3 885761 glnA3 Mycobacterium tuberculosis H37Rv Probable glutamine synthetase GlnA3 (glutamine synthase) (GS-I) NP_216394.1 2128022 D 83332 CDS NP_216395.1 15609016 885650 2129377..2130513 1 NC_000962.3 Rv1879, (MTCY180.39c), len: 378 aa. Conserved hypothetical protein, similar to SCC22.14c|AL096839 hypothetical protein from Streptomyces coelicolor (368 aa),FASTA results: opt: 772, E(): 0 (40.3% identity in 372 aa overlap); and to N-terminal half of nodulin/glutamate-ammonia ligase-like protein. Some similarity to N-terminus of AL132958|ATT4D2_11 Arabidopsis thaliana (845 aa), FASTA results: opt: 354, E(): 3.1e-16,(29.2% identity in 383 aa overlap); and to P38094|FLUG_EMENI Flug protein of Emericella nidulans (865 aa), FASTA results: opt: 306, E(): 6.2e-13, (26.5% identity in 415 aa overlap). Note that the upstream ORF Rv1878|MTCY18 0.40c is similar to the C-terminus.; hypothetical protein 2130513 885650 Rv1879 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216395.1 2129377 D 83332 CDS NP_216396.3 448824767 885758 complement(2130541..2131857) 1 NC_000962.3 Rv1880c, (MT1929, MTCY180.38), len: 438 aa. Probable cyp140, cytochrome p450. Similar to Q00441|CPXJ_SACER 6-deoxyerythronolide beta hydroxylase (404 aa), FASTA scores: opt: 775, E(): 0, (44.2% identity in 319 aa overlap); and other members of the cytochrome P450 family. Related to Mycobacterium tuberculosis proteins include: Rv0766c, Rv2266, Rv0778, etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family.; Probable cytochrome P450 140 Cyp140 2131857 cyp140 885758 cyp140 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 140 Cyp140 NP_216396.3 2130541 R 83332 CDS NP_216397.1 15609018 885762 complement(2131907..2132329) 1 NC_000962.3 Rv1881c, (MTCY180.37), len: 140 aa. Possible lppE,lipoprotein, showing some similarity to L12238|MSG18S19K_1 19K antigen from Mycobacterium intracellulare (162 aa),FASTA scores: opt: 137, E(): 0.0069, (27.6% identity in 156 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Possible conserved lipoprotein LppE 2132329 lppE 885762 lppE Mycobacterium tuberculosis H37Rv Possible conserved lipoprotein LppE NP_216397.1 2131907 R 83332 CDS NP_216398.1 15609019 885782 complement(2132370..2133203) 1 NC_000962.3 Rv1882c, (MTCY180.36), len: 277 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, generally belonging to SDR family, e.g. NP_250789.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (251 aa); NP_421760.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (270 aa); NP_107167.1|NC_002678 oxidoreductase (short chain dehydrogenase/reductase family) from Mesorhizobium loti (253 aa); P50197|LINC_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (250 aa), FASTA scores: opt: 301, E(): 2.3e-12, (30.0% identity in 223 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3057c, Rv1245, etc. Contains possible helix-turn-helix motif at aa 246-267 (+4.32 SD). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 2133203 885782 Rv1882c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_216398.1 2132370 R 83332 CDS NP_216399.1 15609020 885484 complement(2133231..2133692) 1 NC_000962.3 Rv1883c, (MTCY180.35), len: 153 aa. Conserved hypothetical protein, some similarity to hypothetical proteins e.g. Rv2778c|AL008967|MTV002.43 from Mycobacterium tuberculosis (156 aa), FASTA score: opt: 212, E(): 3.1e-08,(34.4% identity in 151 aa overlap). Also similar to U75434|SAU75434_3 Nsh-OrfB from Streptomyces actuosus (173 aa), FASTA score: opt: 207, E(): 1.8e-07, (40.2% identity in 102 aa overlap).; hypothetical protein 2133692 885484 Rv1883c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216399.1 2133231 R 83332 CDS NP_216400.1 15609021 885759 complement(2133731..2134261) 1 NC_000962.3 Rv1884c, (MTCY180.34), len: 176 aa. Probable rpfC,resuscitation promoting factor (see citation below),similar to Z96935|MLRPF_1 resusicitation-promoting factor from Micrococcus luteus (220 aa), FASTA score: opt: 287,E() : 3.3e-11, (40.0% identity in 120 aa overlap). Also similar to others from Mycobacterium tuberculosis: Rv2389c|MTCY253.32|RPFD probable resuscitation-promoting factor (154 aa), FASTA score: opt: 382, E(): 7.1e-17,(55.4% identity in 101 aa overlap); Rv0867c|RPFA (N-terminal part), Rv2450c|RPFE, and Rv1009|RPFB (C-terminal part). Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable resuscitation-promoting factor RpfC 2134261 rpfC 885759 rpfC Mycobacterium tuberculosis H37Rv Probable resuscitation-promoting factor RpfC NP_216400.1 2133731 R 83332 CDS NP_216401.1 15609022 885772 complement(2134273..2134872) 1 NC_000962.3 Rv1885c, (MTCY180.33), len: 199 aa. Chorismate mutase, AroQ class (See Prakash et al., 2005, Sasso et al.,2005), some similarity to P42517|CHMU_ERWHE monofunctional chorismate mutase (181 aa), FASTA score: opt: 181, E(): 0.00017, (28.6% identity in 133 aa overlap). Contains N-terminal signal sequence.; Chorismate mutase 2134872 885772 Rv1885c Mycobacterium tuberculosis H37Rv Chorismate mutase NP_216401.1 2134273 R 83332 CDS NP_216402.1 15609023 885785 complement(2134890..2135867) 1 NC_000962.3 Rv1886c, (MT1934, MTCY180.32), len: 325 aa. FbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO antigen 85-B precursor from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA antigen 85-B precursor from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). Predicted possible vaccine candidate (See Zvi et al., 2008).; Secreted antigen 85-B FbpB (85B) (antigen 85 complex B) (mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen) 2135867 fbpB 885785 fbpB Mycobacterium tuberculosis H37Rv Secreted antigen 85-B FbpB (85B) (antigen 85 complex B) (mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen) NP_216402.1 2134890 R 83332 CDS NP_216403.1 15609024 885247 2136258..2137400 1 NC_000962.3 Rv1887, (MTCY180.31), len: 380 aa. Hypothetical unknown protein; contains eukaryotic thiol (cysteine) proteases histidine active site at N-terminus (PS00639) and Pro-rich region near C-terminus.; Hypothetical protein 2137400 885247 Rv1887 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216403.1 2136258 D 83332 CDS NP_216404.1 15609025 885267 complement(2137519..2138079) 1 NC_000962.3 Rv1888c, (MTCY180.30), len: 186 aa. Possible transmembrane protein.; Possible transmembrane protein 2138079 885267 Rv1888c Mycobacterium tuberculosis H37Rv Possible transmembrane protein NP_216404.1 2137519 R 83332 CDS YP_177654.1 57116927 3205115 complement(2138444..2138617) 1 NC_000962.3 Rv1888A, len: 57 aa. Conserved hypothetical protein. Possibly continuation of Rv1889c, part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~ 120 aa. Includes: C-terminus of Rv0726c|P95074 conserved hypothetical protein (367 aa),FASTA scores: opt: 295, E(): 3.1e-15, (73.684% identity in 57 aa overlap); C-terminus of Rv3399|Q50726|MTCY78.29c conserved hypothetical protein (348 aa), FASTA scores: opt: 504, E(): 7.3e-29, (64.2% identity in 120 aa overlap); C-terminus of Rv0731c; etc.; hypothetical protein 2138617 3205115 Rv1888A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177654.1 2138444 R 83332 CDS NP_216405.1 15609026 885788 complement(2138661..2139017) 1 NC_000962.3 Rv1889c, (MTCY180.29), len: 118 aa. Conserved hypothetical protein. Part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~120 aa. Includes: Rv3399|Q50726|MTCY78.29C conserved hypothetical protein (348 aa), FASTA results: opt: 504,E(): 7.3e-29, (64.2% identity in 120 aa overlap); Rv0726c|P95074; Rv0731c; etc. Rv1888A possibly continuation of this CDS.; hypothetical protein 2139017 885788 Rv1889c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216405.1 2138661 R 83332 CDS NP_216406.1 15609027 885786 complement(2139076..2139687) 1 NC_000962.3 Rv1890c, (MTCY180.28), len: 203 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008).; Hypothetical protein 2139687 885786 Rv1890c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216406.1 2139076 R 83332 CDS NP_216407.1 15609028 885094 2139741..2140148 1 NC_000962.3 Rv1891, (MTCY180.27c), len: 135 aa. Conserved protein. Equivalent to MLCB561.09|AL049571 hypothetical protein from Mycobacterium leprae (134 aa), FASTA scores: opt: 800, E(): 0, (79.7% identity in 133 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2140148 885094 Rv1891 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216407.1 2139741 D 83332 CDS NP_216408.1 15609029 885090 2140165..2140476 1 NC_000962.3 Rv1892, (MTCY180.26c), len: 103 aa. Probable membrane protein.; Probable membrane protein 2140476 885090 Rv1892 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216408.1 2140165 D 83332 CDS NP_216409.1 15609030 885269 2140486..2140704 1 NC_000962.3 Rv1893, (MTCY180.25c), len: 72 aa. Conserved hypothetical protein. Equivalent to MLCB561.11|AL049571 hypothetical protein from Mycobacterium leprae (74 aa),FASTA scores: opt: 317, E(): 4.6e-15, (69.4% identity in 72 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2140704 885269 Rv1893 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216409.1 2140486 D 83332 CDS NP_216410.1 15609031 885081 complement(2140739..2141869) 1 NC_000962.3 Rv1894c, (MTCY180.24), len: 376 aa. Conserved hypothetical protein, weak similarity to some oxidoreductases e.g. Q01284 2-nitropropane dioxygenase precursor (378 aa), FASTA results: opt: 204, E(): 5.8e-06,(34.3% identity in 140 aa overlap). Similar to hypothetical Mycobacterium tuberculosis proteins e.g. Rv3553|MTCY03C7.02c (355 aa), FASTA results: opt: 296, E(): 1.6e-10, (32.9% identity in 167 aa overlap); Rv1533 (375 aa) (48.1% identity in 376 aa overlap); Rv0021c, Rv2781c.; hypothetical protein 2141869 885081 Rv1894c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216410.1 2140739 R 83332 CDS NP_216411.1 15609032 885148 2142521..2143675 1 NC_000962.3 Rv1895, (MTCY180.23c), len: 384 aa. Possible dehydrogenase, similar to various sorbitol and alcohol dehydrogenases, and to putative glutathione-dependent aldehyde dehydrogenase e.g DHSO_BACSU|Q06004 Sorbitol dehydrogenase from Streptomyces coelicolor (352 aa), FASTA results: opt: 506, E(): 7.2e-24, (30.6% identity in 350 aa overlap); and AL109962|SCJ1.28 putative zinc-containing dehydrogenase from Streptomyces coelicolor (356 aa), FASTA results: opt: 634, E(): 2.9e-30, (34.7% identity in 357 aa overlap). Also similar to other Mycobacterium tuberculosis dehydrogenases. Note that there is a substantial (134 bp) overlap at the C-terminus with the C-terminus of the downstream ORF, although both appear to be true coding regions.; Possible dehydrogenase 2143675 885148 Rv1895 Mycobacterium tuberculosis H37Rv Possible dehydrogenase NP_216411.1 2142521 D 83332 CDS NP_216412.1 15609033 885915 complement(2143535..2144446) 1 NC_000962.3 Rv1896c, (MTCY180.22), len: 303 aa. Conserved hypothetical protein. Similar to several (14) hypothetical Mycobacterium tuberculosis proteins e.g. Rv0145|MTCI5.19 (317 aa), FASTA results: opt: 720, E(): 0, (41.6% identity in 308 aa overlap); Q10552|YZ21_MYCTU (325 aa), opt: 689,E(): 0, (40.5% identity in 304 aa overlap); Rv0726c,Rv0731c, Rv3399, etc. and to related proteins in other actinomycetes. Note that there is a substantial (134 bp) overlap at the C-terminus with the C-terminus of the downstream ORF, although both appear to be true coding regions.; hypothetical protein 2144446 885915 Rv1896c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216412.1 2143535 R 83332 CDS NP_216413.1 15609034 885893 complement(2144451..2144882) 1 NC_000962.3 Rv1897c, (MTCY180.21), len: 143 aa. Conserved hypothetical protein. Some similarity to D63706|Q54235 ORF2 from Streptomyces griseus (149 aa), FASTA results: opt: 509, E(): 1.2e-28, (57.3% identity in 150 aa overlap); and Q45303 ORF1 protein from Corynebacterium glutamicum (144 aa), FASTA results: opt: 460, E(): 5.5e-23, (49.7% identity in 143 aa overlap).; hypothetical protein 2144882 885893 Rv1897c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216413.1 2144451 R 83332 CDS NP_216414.1 15609035 885089 2144940..2145248 1 NC_000962.3 Rv1898, (MTCY180.20c), len: 102 aa. Conserved hypothetical protein, some similarity to other hypothetical proteins e.g. Q58452 from methanococcus jannasch II (100 aa), FASTA results: opt: 152, E(): 9.1e-05, (31.5% identity in 92 aa overlap); and AE000771|AE000771_2 from Aquifex aeolicus (157 aa), FASTA results: opt: 246, E(): 3.2e-11,(39.0% identity in 100 aa overlap).; hypothetical protein 2145248 885089 Rv1898 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216414.1 2144940 D 83332 CDS NP_216415.1 15609036 885138 complement(2145214..2146245) 1 NC_000962.3 Rv1899c, (MTCY180.19), len: 343 aa. Possible lipoprotein; contains appropriately localized lipoprotein lipid attachment site (PS00013). Some similarity to C-terminal part of AE000717|AE000717_4 hypothetical protein from Aquifex aeolicus section 49 (165 aa), FASTA results: opt: 372, E(): 2.3e-14, (43.5% identity in 147 aa overlap); and Q44020 4-hydroxybutyrate dehydrogenase (173 aa), FASTA results: opt: 272, E(): 4.7e-09, (35.8% identity in 165 aa overlap).; Possible lipoprotein LppD 2146245 lppD 885138 lppD Mycobacterium tuberculosis H37Rv Possible lipoprotein LppD NP_216415.1 2145214 R 83332 CDS NP_216416.1 15609037 885151 complement(2146245..2147633) 1 NC_000962.3 Rv1900c, (MTCY180.18), len: 462 aa. Probable lipJ,lignin peroxidase, with some similarity to esterases,hydrolases and hypothetical Mycobacterium tuberculosis proteins e.g. Q43936 beta-ketoadipate enol-lactone hydrolase from Acinetobacter calcoaceticus (267 aa), FASTA results: opt: 217, E(): 1.7e-07, (29.2% identity in 260 aa overlap). Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv2212|Q10400|YM12_MYCTU (378 aa), FASTA results: opt: 216, E(): 6.7e-07, (27.7% identity in 285 aa overlap).; Probable lignin peroxidase LipJ 2147633 lipJ 885151 lipJ Mycobacterium tuberculosis H37Rv Probable lignin peroxidase LipJ NP_216416.1 2146245 R 83332 CDS NP_216417.1 15609038 885149 2147662..2148954 1 NC_000962.3 Rv1901, (MTCY180.17c), len: 430 aa. Probable cinA-like protein, strong similarity to competence damage proteins CinA of Bacillus subtilis and S. pneumoniae. FASTA results: Q55760 hypothetical 44.7 kDa protein (416 aa) opt: 755, E(): 0, (36.0% identity in 433 aa overlap).; Probable CinA-like protein CinA 2148954 cinA 885149 cinA Mycobacterium tuberculosis H37Rv Probable CinA-like protein CinA NP_216417.1 2147662 D 83332 CDS NP_216418.1 15609039 885057 complement(2149006..2150274) 1 NC_000962.3 Rv1902c, (MTCY180.16), len: 422 aa. Probable nanT,sialic acid-transport integral membrane protein, possibly member of major facilitator superfamily (MFS), similar to others e.g. Q48076 sialic acid transporter (407 aa), FASTA results: opt: 443, E(): 5.4e-22, (26.7% identity in 389 aa overlap); etc. Some similarity to MTCI364.12|O05301 conserved hypothetical protein from Mycobacterium tuberculosis (425 aa), FASTA results: opt: 251, E(): 1.1e-09, (23.5% identity in 417 aa overlap). Contains sugar transport proteins signature 2 (PS00217).; Probable sialic acid-transport integral membrane protein NanT 2150274 nanT 885057 nanT Mycobacterium tuberculosis H37Rv Probable sialic acid-transport integral membrane protein NanT NP_216418.1 2149006 R 83332 CDS NP_216419.1 15609040 885643 2150364..2150768 1 NC_000962.3 Rv1903, (MTCY180.15c), len: 134 aa. Probable conserved membrane protein, similar to Q53868|YPT3_STRCO hypothetical 15.9 kDa protein from Streptomyces coelicolor (148 aa) opt: 323, E(): 1.3e-16, (42.9% identity in 126 aa overlap); and equivalent to AJ000521|MLCOSL672_3 from Mycobacterium leprae (139 aa), FASTA results: opt: 680,E(): 0, (80.6% identity in 129 aa overlap).; Probable conserved membrane protein 2150768 885643 Rv1903 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_216419.1 2150364 D 83332 CDS NP_216420.1 15609041 885281 2150954..2151385 1 NC_000962.3 Rv1904, (MTCY180.14c), len: 143 aa. Conserved hypothetical protein, some similarity to other hypothetical Mycobacterium tuberculosis proteins e.g. Rv2638|MTCY441.08|P71937 (148 aa), FASTA results: opt: 456,E(): 2.7e-23, (52.8% identity in 125 aa overlap); Rv1365|Q11035 (128 aa), FASTA results: opt: 393, E(): 1.4e-19, (48.8% identity in 123 aa overlap); and Rv3687c. Also weak similarity to Q9WVX8|RSBV_STRCO anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa).; hypothetical protein 2151385 885281 Rv1904 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216420.1 2150954 D 83332 CDS NP_216421.1 15609042 885504 complement(2151433..2152395) 1 NC_000962.3 Rv1905c, (MTCY180.13), len: 320 aa. Probable aao,D-amino acid oxidase, similar to many. Equivalent to AJ000521|MLCOSL672.02|O33145 Mycobacterium leprae (320 aa),FASTA results: opt: 1541, E(): 0, (71.7% identity in 315 aa overlap); also similar to OXDD_BOVIN|P31228 d-aspartate oxidase from bos taurus (338 aa), FASTA results: opt: 461,E(): 1.1e-21, (31.8% identity in 321 aa overlap).; Probable D-amino acid oxidase Aao 2152395 aao 885504 aao Mycobacterium tuberculosis H37Rv Probable D-amino acid oxidase Aao NP_216421.1 2151433 R 83332 CDS NP_216422.1 15609043 885514 complement(2152425..2152895) 1 NC_000962.3 Rv1906c, (MTCY180.12), len: 156 aa. Conserved protein, possibly exported protein, equivalent to Mycobacterium leprae AJ000521|MLCOSL672.01 (153 aa), FASTA scores: opt: 637, E(): 2.6e-28, (63.2% identity in 155 aa overlap). Also similar to M. tuberculosis hypothetical exported protein, Rv1352. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 2152895 885514 Rv1906c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216422.1 2152425 R 83332 CDS NP_216423.1 15609044 885402 complement(2153235..2153882) 1 NC_000962.3 Rv1907c, (MTCY180.11), len: 215 aa. Hypothetical unknown protein. Similar to Q50763 Ethyl methane sulphonate resistance protein from Mycobacterium tuberculosis (168 aa), FASTA scores: opt: 638, E(): 0, (69.7% identity in 152 aa overlap). Downstream of a cloned katG gene (EMBL:mtkatg). Differences are due to frameshift errors in the EMBL sequence and the use of an earlier start codon. Alternative nucleotide at position 2153410 (a->G; V158A) has been observed.; Hypothetical protein 2153882 885402 Rv1907c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216423.1 2153235 R 83332 CDS NP_216424.1 15609045 885638 complement(2153889..2156111) 1 NC_000962.3 Rv1908c, (MTCY180.10), len: 740 aa. KatG,catalase-peroxidase-peroxynitritase T (see citations below), HPI. FASTA results: Q57215 catalase-peroxidase from Mycobacterium tuberculosis (740 aa) opt: 5081, E(): 0,(100% identity in 740 aa overlap). Contains peroxidases active site signature (PS00436) and ATP/GTP-binding site motif A (P-loop; PS00017). Cosmid sequence was corrected to agree with a sequencing read from the H37Rv genome. Deletions or defects in KATG gene cause isoniazid (INH) resistance. Belongs to the peroxidase family. Bacterial peroxidase/catalase subfamily. KATG transcription seems to be regulated by FURA|Rv1909c product. The catalase-peroxidase activity is associated with the amino-terminal domain but no definite function has been assigned to the carboxy-terminal domain. Predicted possible vaccine candidate (See Zvi et al., 2008).; Catalase-peroxidase-peroxynitritase T KatG 2156111 katG 885638 katG Mycobacterium tuberculosis H37Rv Catalase-peroxidase-peroxynitritase T KatG NP_216424.1 2153889 R 83332 CDS NP_216425.2 448824768 885400 complement(2156149..2156592) 1 NC_000962.3 Rv1909c, (MTCY180.09), len: 147 aa. FurA, Ferric uptake regulation protein, similar to Q48835 legionella pneumophila 130B (wadsworth) ferric uptake regulation (136 aa), FASTA results: opt: 230, E(): 2.5e-09, (32.3% identity in 133 aa overlap). Also similar to Mycobacterium tuberculosis zur zinc uptake regulatory protein, Rv2359. Belongs to the fur family. Start changed since original submission (-3 aa).; Ferric uptake regulation protein FurA (fur) 2156592 furA 885400 furA Mycobacterium tuberculosis H37Rv Ferric uptake regulation protein FurA (fur) NP_216425.2 2156149 R 83332 CDS NP_216426.1 15609047 885897 complement(2156706..2157299) 1 NC_000962.3 Rv1910c, (MTCY180.08), len: 197 aa. Possible exported protein, very similar to upstream ORF MTCY180.07 (201 aa), FASTA score: E(): 0, (64.0% identity in 200 aa overlap). Also similar to Q9Z729|Y877_CHLPN protein CPN0877 from Chlamydophila pneumoniae (150 aa). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable exported protein 2157299 885897 Rv1910c Mycobacterium tuberculosis H37Rv Probable exported protein NP_216426.1 2156706 R 83332 CDS NP_216427.1 15609048 885646 complement(2157382..2157987) 1 NC_000962.3 Rv1911c, (MTCY180.07), len: 201 aa. Probable lipoprotein lppC, contains appropriately positioned prokaryotic membrane lipoprotein lipid attachment site (PS00013). Very similar to downstream ORF MTCY180.08 (204 aa) (although this lacks lipoprotein motif), FASTA score: opt: 831, E(): 0, (64.0% identity in 200 aa overlap). Also similar to Q9Z729|Y877_CHLPN hypothetical protein CPN0877 from Chlamydia pneumoniae (strain CWL029) (150 aa).; Probable lipoprotein LppC 2157987 lppC 885646 lppC Mycobacterium tuberculosis H37Rv Probable lipoprotein LppC NP_216427.1 2157382 R 83332 CDS NP_216428.1 15609049 885245 complement(2158087..2159091) 1 NC_000962.3 Rv1912c, (MTCY180.06), len: 334 aa. Possible fadB5,oxidoreductase, similar to various oxidoreductases: 3-hydroxyacyl-CoA dehydrogenase, quinone oxidoreductases,and polyketide synthases, e.g. NP_104067.1|NC_002678 probable oxidoreductase from Mesorhizobium loti (308 aa); NP_464140.1|NC_003210 protein similar to oxidoreductase from Listeria monocytogenes (313 aa); NP_193889.1|NC_003075 putative NADPH quinone oxidoreductase from Arabidopsis thaliana (325 aa); NP_001880.2|NM_001889 crystallin, zeta; quinone oxidoreductase; NADPH:quinone reductase from Homo sapiens (329 aa); part 2983 to 3197 of T17410 polyketide synthase type I from Streptomyces venezuelae (3739 aa); Q53927|SCBAC20F6.16 hydroxyacyl-CoA dehydrogenase from Streptomyces coelicolor (329 aa), FASTA scores: opt: 621,E(): 2e-30, (39.5% identity in 349 aa overlap); etc. Also similar to many hypothetical Mycobacterium tuberculosis proteins including: MTCY24G1.09, MTCY13D12.11, MTCY19H9.01,MTCY24G1.03, MTCY03A2.17c, etc. Contains quinone oxidoreductase/zeta-crystallin signature (PS01162).; Possible oxidoreductase FadB5 2159091 fadB5 885245 fadB5 Mycobacterium tuberculosis H37Rv Possible oxidoreductase FadB5 NP_216428.1 2158087 R 83332 CDS NP_216429.1 15609050 885640 2159191..2159943 1 NC_000962.3 Rv1913, (MTCY180.05c), len: 250 aa. Conserved hypothetical protein, slight similarity to dehydrase and beta-lactamase precursors e.g. Q02057 dehydrase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 184,E(): 4.3e-05, (31.6% identity in 215 aa overlap).; hypothetical protein 2159943 885640 Rv1913 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216429.1 2159191 D 83332 CDS NP_216430.1 15609051 885875 complement(2159921..2160328) 1 NC_000962.3 Rv1914c, (MTCY180.04), len: 135 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 2160328 885875 Rv1914c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216430.1 2159921 R 83332 CDS NP_216431.1 15609052 885639 2160463..2161566 1 NC_000962.3 Rv1915, (MTCY180.03c), len: 367 aa. Probable aceAa,isocitrate lyase (see citations below). Highly similar to the N-terminus of ACEA_MYCLE isocitrate lyase from Mycobacterium leprae (606 aa), FASTA results: opt: 3314,E(): 0, (86.5% identity in 572 aa overlap). Contains PS00161 Isocitrate lyase signature. Although this ORF and the downstream ORF representing the C-terminal half of aceA could be joined by a frameshift, no error is apparent in the cosmid, or in a seqencing read from the genome of H37Rv. As the downstream ORF has a RBS and transcriptional start immediately following the stop of this ORF, it is possible that they are expressed as two separate modules. In Mycobacterium tuberculosis strain CDC1551, aceA exists as a single gene, MT1966: the corresponding protein has been purified experimentally and seems have an active isocitrate lyase activity (see Honer et al., 1999). For Mycobacterium tuberculosis strain H37Rv, immunoblot assay didn't detect AceAa or AceAb products (see Honer et al.,1999) but mRNA of AceAa|Rv1915 has been detected (see Betts et al., 2002); so AceAb|Rv1916 could be a pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006).; Probable isocitrate lyase AceAa [first part] (isocitrase) (isocitratase) (Icl) 2161566 aceAa 885639 aceAa Mycobacterium tuberculosis H37Rv Probable isocitrate lyase AceAa [first part] (isocitrase) (isocitratase) (Icl) NP_216431.1 2160463 D 83332 CDS NP_216432.1 15609053 885383 2161566..2162762 1 NC_000962.3 Rv1916, (MTCY180.02c), len: 398 aa. Probable aceAb,isocitrate lyase (see citations below). Highly similar to the C-terminus of ACEA_MYCLE|P46831 isocitrate lyase from Mycobacterium leprae (606 aa), FASTA results: opt: 1635,E(): 0, (86.3% identity in 278 aa overlap). Although this ORF and the upstream ORF representing the N-terminal half of aceA could be joined by a frameshift no error is apparent in the cosmid, or in a seqencing read from the genome of H37Rv. As this ORF has a RBS and transcriptional start immediately following the stop of the upstream ORF,it is possible that they are expressed as two separate modules. In Mycobacterium tuberculosis strain CDC1551, aceA exists as a single gene, MT1966: the corresponding protein has been purified experimentally and seems have an active isocitrate lyase activity (see Honer et al., 1999). For Mycobacterium tuberculosis strain H37Rv, immunoblot assay didn't detect AceAa or AceAb products (see Honer et al.,1999) but mRNA of AceAa|Rv1915 has been detected (see Betts et al., 2002); so AceAb|Rv1916 could be a pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006).; Probable isocitrate lyase AceAb [second part] (isocitrase) (isocitratase) (Icl) 2162762 aceAb 885383 aceAb Mycobacterium tuberculosis H37Rv Probable isocitrate lyase AceAb [second part] (isocitrase) (isocitratase) (Icl) NP_216432.1 2161566 D 83332 CDS YP_177655.1 57116928 885362 complement(2162932..2167311) 1 NC_000962.3 Rv1917c, (MTV050.01c-MTCY180.01), len: 1459 aa. PPE34, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, MPTR subfamily (see citation below). Similar to MTCY28.16, MTCY13E10.17, MTCY63.10,MTV004.05, MTCY98.24, MTCY6G11.05, etc. C-terminus is identical to Q50471. Unknown Mycobacterium tuberculosis protein (693 aa), FASTA results: opt: 2635, E(): 0, (99.7% identity in 391 aa overlap). Start changed since original submission (+23 aa). Thougth to be surface exposed,cell-wall associated.; PPE family protein PPE34 2167311 PPE34 885362 PPE34 Mycobacterium tuberculosis H37Rv PPE family protein PPE34 YP_177655.1 2162932 R 83332 CDS YP_177850.1 57116929 885506 complement(2167649..2170612) 1 NC_000962.3 Rv1918c, (MTV050.02c), len: 987 aa. PPE35, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins. Similar to MTCY28.16|Z95890 Mycobacterium tuberculosis cosmid (1053 aa), FASTA scores: opt: 3404,E(): 0, (65.6% identity in 1058 aa overlap). Also similar to MTV004.05, MTY13E10.17, MTV014.03, MTCY3C7.23,MTCY6G11.05, MTCY48.17, MTV004.03, MTCY31.07, MTCY4C12.36,MTCY180.01, etc.; PPE family protein PPE35 2170612 PPE35 885506 PPE35 Mycobacterium tuberculosis H37Rv PPE family protein PPE35 YP_177850.1 2167649 R 83332 CDS NP_216435.1 15609056 885876 complement(2171061..2171525) 1 NC_000962.3 Rv1919c, (MTV050.03c), len: 154 aa. Conserved protein, shows weak similarity to several major pollen antigens e.g. Z72431|BVGC25_1 major allergen bet V 1 from Betula verrucosa (160 aa), FASTA scores: opt: 133, E(): 0.012, (26.8% identity in 149 aa overlap). Also shows some similarity to Rv2574|MTCY227.27C Hypothetical protein from Mycobacterium tuberculosis (167 aa), (27.4% identity in 124 aa overlap).; hypothetical protein 2171525 885876 Rv1919c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216435.1 2171061 R 83332 CDS NP_216436.1 15609057 885416 2171623..2172486 1 NC_000962.3 Rv1920, (MTV050.04), len: 287 aa. Probable membrane protein, similar to AL0215|SC10A5.04 putative membrane protein from Streptomyces coelicolor cosmid 10A5 (295 aa),FASTA scores: opt: 292, E(): 3.6e-13, (31.3% identity in 243 aa overlap). Also weakly similar to several Mycobacterial putative proteins with unknown function e.g. Rv0502, Rv1428c, U00018_22 Mycobacterium leprae cosmid B2168.; Probable membrane protein 2172486 885416 Rv1920 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216436.1 2171623 D 83332 CDS NP_216437.1 15609058 885917 complement(2172524..2173795) 1 NC_000962.3 Rv1921c, (MTCY09F9.43-MTV050.05c), len: 423 aa. Probable lppF, conserved lipoprotein, similar to G403173 lipoprotein precursor (fragment) from Rhodococcus erythropolis (225 aa), fasta scores: opt: 364, E(): 9.2e-19, (41.9% identity in 148 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LppF 2173795 lppF 885917 lppF Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppF NP_216437.1 2172524 R 83332 CDS NP_216438.1 15609059 885918 2174067..2175182 1 NC_000962.3 Rv1922, (MTCY09F9.42c), len: 371 aa. Probable conserved lipoprotein, possibly peptidase similar to many peptidases, e.g. P15555|DAC_STRSQ D-alanyl-D-alanine carboxypeptidase from Streptomyces sp. (406 aa), FASTA scores: opt: 382, E(): 3.1e-17, (28.0% identity in 379 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1497, Rv2463, Rv3775, etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein 2175182 885918 Rv1922 Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein NP_216438.1 2174067 D 83332 CDS NP_216439.1 15609060 885910 2175173..2176513 1 NC_000962.3 Rv1923, (MTCY09F9.41c), len: 446 aa. Probable lipD,hydrolase lipase, similar to esterases and beta-lactamases e.g. G151214 esterase, (389 aa), fasta scores: opt: 569,E(): 5.4e-29, (33.7% identity in 401 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1497, Rv2463, Rv3775, etc.; Probable lipase LipD 2176513 lipD 885910 lipD Mycobacterium tuberculosis H37Rv Probable lipase LipD NP_216439.1 2175173 D 83332 CDS NP_216440.1 15609061 885352 complement(2176550..2176930) 1 NC_000962.3 Rv1924c, (MTCY09F9.40), len: 126 aa. Unknown protein.; hypothetical protein 2176930 885352 Rv1924c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216440.1 2176550 R 83332 CDS NP_216441.2 448824769 885200 2177087..2178949 1 NC_000962.3 Rv1925, (MTCY09F9.39c), len: 620 aa. Probable fadD31, acyl-CoA synthetase, highly similar to others from Mycobacterium leprae e.g. NP_301198.1|NC_002677 putative acyl-CoA synthetase (635 aa); NP_302537.1|NC_002677 probable acyl-CoA synthase (583 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD32 (637 aa); fadD21 (578 aa); fadD29 (619 aa); fadD26|FD26_MYCTU|Q10976 (626 aa), FASTA scores: opt: 945,E(): 0, (39.8% identity in 598 aa overlap); etc. Also similar to N-terminus of G1171128 saframycin MX1 synthetase B from Myxococcus xanthus (1770 aa), FASTA scores: opt: 845, E(): 0, (37.4% identity in 593 aa overlap); N-terminus of T34918 polyketide synthase from Streptomyces coelicolor (2297 aa); etc. Nucleotide position 2177654 in the genome sequence has been corrected, A:C resulting in M190L.; Probable acyl-CoA ligase FadD31 (acyl-CoA synthetase) (acyl-CoA synthase) 2178949 fadD31 885200 fadD31 Mycobacterium tuberculosis H37Rv Probable acyl-CoA ligase FadD31 (acyl-CoA synthetase) (acyl-CoA synthase) NP_216441.2 2177087 D 83332 CDS NP_216442.1 15609063 885334 complement(2178957..2179436) 1 NC_000962.3 Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. Mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al., 2008).; Immunogenic protein Mpt63 (antigen Mpt63/MPB63) (16 kDa immunoprotective extracellular protein) 2179436 mpt63 885334 mpt63 Mycobacterium tuberculosis H37Rv Immunogenic protein Mpt63 (antigen Mpt63/MPB63) (16 kDa immunoprotective extracellular protein) NP_216442.1 2178957 R 83332 CDS NP_216443.1 15609064 885912 2179673..2180446 1 NC_000962.3 Rv1927, (MTCY09F9.37c), len: 257 aa. Conserved hypothetical protein, similar to SCG11A.10c|AL133210 hypothetical protein from Streptomyces coelicolor (252 aa),FASTA scores: opt: 729, E(): 0, (48.3% identity in 238 aa overlap). Slight similarity with P54543|YQJF_BACSU hypothetical 23.9 kDa protein from Bacillus subtilis (209 aa), FASTA scores, opt: 230, E(): 2.8e-08, (28.0% identity in 164 aa overlap).; hypothetical protein 2180446 885912 Rv1927 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216443.1 2179673 D 83332 CDS NP_216444.1 15609065 885331 complement(2180450..2181217) 1 NC_000962.3 Rv1928c, (MTCY09F9.36), len: 255 aa. Probable short-chain dehydrogenase/reductase, highly similar to others e.g. NP_228109.1|NC_000853 oxidoreductase (short chain dehydrogenase/reductase family) from Thermotoga maritima (257 aa); T41116 short chain dehydrogenase from Schizosaccharomyces pombe (261 aa); P87219|SOU1_CANAL sorbitol utilization protein (SDR family) from Candida albicans (281 aa); P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 541, E(): 1.2e-27, (37.5% identity in 251 aa overlap); etc. Also similar to many mycobacterial tuberculosis proteins e.g. Rv1350, Rv0927c, Rv2002, Rv0769, Rv2766c,etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 2181217 885331 Rv1928c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_216444.1 2180450 R 83332 CDS NP_216445.1 15609066 885630 complement(2181262..2181906) 1 NC_000962.3 Rv1929c, MTCY09F9.35, len: 214 aa. Conserved hypothetical protein, similar to SC4G6.14|AL096884 hypothetical protein from Streptomyces coelicolor (211 aa),FASTA scores: opt: 416, E(): 2.4e-22, (39.8% identity in 206 aa overlap).; hypothetical protein 2181906 885630 Rv1929c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216445.1 2181262 R 83332 CDS NP_216446.1 15609067 885344 complement(2181918..2182442) 1 NC_000962.3 Rv1930c, MTCY09F9.34, len: 174 aa. Conserved hypothetical protein, similar to SC5F2A.30|AL049587 hypothetical protein from Streptomyces coelicolor (211 aa),FASTA scores: opt: 307, E(): 2.8e-13, (54.8% identity in 84 aa overlap). Some similarity to M. tuber culosis hypothetical protein Rv0052|MTCY21D4.15 (43% identity in 93 aa overlap).; hypothetical protein 2182442 885344 Rv1930c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216446.1 2181918 R 83332 CDS NP_216447.1 15609068 885437 complement(2182460..2183239) 1 NC_000962.3 Rv1931c, (MTCY09F9.33), len: 259 aa. Probable transcriptional regulatory protein. Similarity in C-terminal half to transcriptional activators e.g. Q43970 AraC-like protein (227 aa), FASTA scores: opt: 238, E(): 7.1e-07, (42.4% identity in 92 aa overlap). Similar to many probable transcription regulators in Streptomyces e.g. AL049587|SC5F2A.29 Streptomyces coelicolor (325 aa), FASTA scores: opt: 387, E(): 3.2e-16, (34.4% identity in 259 aa overlap).; Probable transcriptional regulatory protein 2183239 885437 Rv1931c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_216447.1 2182460 R 83332 CDS NP_216448.1 15609069 885357 2183372..2183869 1 NC_000962.3 Rv1932, (MTCY09F9.32c), len: 165 aa. Probable tpx (alternate gene name: cfp20), thiol peroxidase similar to TPX_ECOLI|P37901 thiol peroxidase (p20) from Escherichia coli (167 aa), fasta scores: opt: 535, E(): 7.3e-25, (52.4% identity in 164 aa overlap). There are four other related enzymes in M. tuberculosis: Rv2428, Rv2521, Rv2238c,Rv1608c.; Probable thiol peroxidase Tpx 2183869 tpx 885357 tpx Mycobacterium tuberculosis H37Rv Probable thiol peroxidase Tpx NP_216448.1 2183372 D 83332 CDS NP_216449.1 15609070 885394 complement(2183866..2184957) 1 NC_000962.3 Rv1933c, (MTCY09F9.31), len: 363 aa. Probable fadE18, acyl-CoA dehydrogenase, similar to many e.g. CAB61609.1|AL133210 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (362 aa); NP_421282.1|NC_002696 acyl-CoA dehydrogenase family protein from Caulobacter crescentus (344 aa); ACDS_RAT|P15651 short-chain specific acyl-CoA dehydrogenase from Rattus norvegicus (Rat) (412 aa), fasta scores: opt: 239, E(): 2.1e-08, (28.4% identity in 331 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. N-terminus of fadE22 (721 aa); fadE33 (318 aa); N-terminus of fadE34 (711 aa); etc. Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE18 2184957 fadE18 885394 fadE18 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE18 NP_216449.1 2183866 R 83332 CDS NP_216450.1 15609071 885379 complement(2184959..2186188) 1 NC_000962.3 Rv1934c, (MTCY09F9.30), len: 409 aa. Probable fadE17, acyl-CoA dehydrogenase, highly similar to ACD_MYCLE|P46703 acyl-CoA dehydrogenase from Mycobacterium leprae (389 aa), FASTA scores: opt: 414, E(): 2.6e-19,(28.3% identity in 407 aa overlap). Also similar to many e.g. NP_249713.1|NC_002516 probable acyl-CoA dehydrogenase from Pseudomonas aeruginosa (381 aa); NP_420614.1|NC_002696 acyl-CoA dehydrogenase family protein from Caulobacter crescentus (355 aa); CAB61610.1|AL133210 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (393 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. fadE30 (385 aa); fadE31 (377 aa); C-terminus of fadE34 (711 aa); etc. Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE17 2186188 fadE17 885379 fadE17 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE17 NP_216450.1 2184959 R 83332 CDS NP_216451.1 15609072 885240 complement(2186203..2187159) 1 NC_000962.3 Rv1935c, (MTCY09F9.29), len: 318 aa. Possible echA13, enoyl-CoA hydratase, similar to others and various enzymes e.g. CAC48381.1|Y16952 putative enoyl-CoA-isomerase from Amycolatopsis mediterranei (269 aa); AAK18173.1|AF290950_5|AF290950|FadB1x enoyl-CoA hydratase from Pseudomonas putida (257 aa); AAF78820.1|AF042490 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. TM1 (276 aa); ECHM_RAT|P14604 enoyl-CoA hydratase mitochondrial precursor from Rattus norvegicus (Rat) (290 aa), FASTA scores: opt: 228, E(): 1.2e-08, (31.0% identity in 258 aa overlap); etc.; Possible enoyl-CoA hydratase EchA13 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 2187159 echA13 885240 echA13 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase EchA13 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_216451.1 2186203 R 83332 CDS NP_216452.1 15609073 885882 2187384..2188493 1 NC_000962.3 Rv1936, (MTCY09F9.28c), len: 369 aa. Possible monooxygenase, similar to LXA2_PHOLU|P23146 alkanal monooxygenase alpha chain (362 aa), FASTA scores: opt: 196,E(): 6.3e-06, (22.3% identity in 373 aa overlap). Also similar to many other Mycobacterium tuberculosis hypothetical oxidoreductases and monooxygenases e.g. Rv0953c, Rv0791c, Rv0132c, etc.; Possible monooxygenase 2188493 885882 Rv1936 Mycobacterium tuberculosis H37Rv Possible monooxygenase NP_216452.1 2187384 D 83332 CDS NP_216453.1 15609074 885433 2188496..2191015 1 NC_000962.3 Rv1937, (MTCY09F9.27c), len: 839 aa. Possible oxygenase, similar in N-terminus to N-terminal part (approx. 350 aa) of dioxygenases (including ring-hydroxylating dioxygenase electron transfer components) and monooxygenases, e.g. AAC34815.1|AF071556 anthranilate dioxygenase reductase from Acinetobacter sp. (343 aa); AAK52291.1|AY026914|AntC putative anthranilate dioxygenase reductase from Pseudomonas putida (340 aa); AAF63450.1|AF218267_7|AF218267 benzoate dioxygenase / ferredoxin reductase from Pseudomonas putida (336 aa); P23101|XYLZ_PSEPU toluate 1,2-dioxygenase electron transfer component [includes: ferredoxin; ferredoxin--NAD(+) reductase ] from Pseudomonas putida plasmid TOL pWW0 (336 aa), FASTA scores: opt: 700, E(): 0, (34.3% identity in 335 aa overlap); S23479 probable benzoate 1,2-dioxygenase reductase component benC from Acinetobacter calcoaceticus (338 aa); AAC45294.1|U81594 soluble methane monooxygenase protein C from Methylocystis sp. (343 aa); P22868|MEMC_METCA methane monooxygenase component C from Methylococcus capsulatus (348 aa); etc. Also similar in part to Mycobacterium tuberculosis hypothetical electron transfer proteins Rv3554, Rv3571, etc. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature.; Possible oxygenase 2191015 885433 Rv1937 Mycobacterium tuberculosis H37Rv Possible oxygenase NP_216453.1 2188496 D 83332 CDS NP_216454.1 15609075 885392 2191027..2192097 1 NC_000962.3 Rv1938, (MTCY09F9.26c), len: 356 aa. Probable ephB,epoxide hydrolase (see citation below), similar to many e.g. G1109600 ATSEH (321 aa), FASTA scores: opt: 442, E(): 1.2e-21 (33.1% identity in 356 aa overlap); etc. Also similar to many other M. tuberculosis hypothetical epoxide hydrolases e.g. Rv3617, Rv3670, Rv0134, etc.; Probable epoxide hydrolase EphB (epoxide hydratase) 2192097 ephB 885392 ephB Mycobacterium tuberculosis H37Rv Probable epoxide hydrolase EphB (epoxide hydratase) NP_216454.1 2191027 D 83332 CDS NP_216455.1 15609076 885488 2192094..2192609 1 NC_000962.3 Rv1939, (MTCY09F9.25c), len: 171 aa. Probable oxidoreductase, similar to NP_302637.1|NC_002677 probable oxidoreductase from Mycobacterium leprae (162 aa) Also similar to NTAB_CHELE|P54990 nitrilotriacetate monooxygenase component from Chelatobacter heintzii (322 aa), fasta scores: opt: 269, E(): 5.3e-11, (33.1% identity in 151 aa overlap). And similar to Mycobacterium tuberculosis probable monooxygenase components Rv0246,Rv3567, and to a lesser extent, Rv3007c.; Probable oxidoreductase 2192609 885488 Rv1939 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_216455.1 2192094 D 83332 CDS YP_177851.1 57116930 885621 2192606..2193667 1 NC_000962.3 Rv1940, (MTCY09F9.24c), len: 353 aa. Probable ribA1,Riboflavin biosynthesis protein, similar to GCH2_BACSU|P17620 gtp cyclohydrolase II (398 aa), FASTA scores: opt: 682, E(): 0, (37.7% identity in 363 aa overlap), also similar to Rv1415|MTCY21B4.33|ribA2 (428 aa) (45.4% identity in 368 aa overlap). Note that previously known as ribA.; Probable riboflavin biosynthesis protein RibA1 (GTP cyclohydrolase II) 2193667 ribA1 885621 ribA1 Mycobacterium tuberculosis H37Rv Probable riboflavin biosynthesis protein RibA1 (GTP cyclohydrolase II) YP_177851.1 2192606 D 83332 CDS NP_216457.1 15609078 885622 2193664..2194434 1 NC_000962.3 Rv1941, (MTCY09F9.23c), len: 256 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, generally belonging to SDR family, e.g. NP_299015.1|NC_002488 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase from Xylella fastidiosa (255 aa); NP_250340.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); NP_106890.1|NC_002678 probable short-chain type dehydrogenase/reductase from Mesorhizobium loti (374 aa) (has its N-terminus longter); P50197|LINC_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-dehydrogenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (250 aa), FASTA scores: opt: 529, E(): 5.7e-25, (40.6% identity in 251 aa overlap); etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 2194434 885622 Rv1941 Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_216457.1 2193664 D 83332 CDS NP_216458.1 15609079 885606 complement(2194644..2194973) 1 NC_000962.3 Rv1942c, (MTCY09F9.22), len: 109 aa. Possible mazF5,toxin, part of toxin-antitoxin (TA) operon with Rv1943c (See Pandey and Gerdes, 2005; Zhu et al., 2006), shows some similarity to Q10867|MTCY39.28|Rv1991 hypothetical 12.3 kDa protein (114 aa), FASTA scores: opt: 117, E(): 0.021, (24. 5% identity in 110 aa overlap) also P33645|CHPA_ECOLI pemk-like protein 1 (mazf protein) from Escherichia coli (111 aa), FASTA scores: opt: 104, E(): 0.18, (29.1% identity in 110 aa overlap). Also similar to Mycobacterium tuberculosis Rv0659c (102 aa) (32.7% identity in 101 aa overlap); Rv1102c (33.3% identity in 93 aa overlap) and Rv1495.; Possible toxin MazF5 2194973 mazF5 885606 mazF5 Mycobacterium tuberculosis H37Rv Possible toxin MazF5 NP_216458.1 2194644 R 83332 CDS NP_216459.1 15609080 885605 complement(2194970..2195347) 1 NC_000962.3 Rv1943c, (MTCY09F9.21), len: 125 aa. Possible mazE5,antitoxin, part of toxin-antitoxin (TA) operon with Rv1942c (See Pandey and Gerdes, 2005; Zhu et al., 2006), shows some similarity with Rv1946c|MTCY09F9.18|lppG possible conserved lipoprotein from Mycobacterium tuberculosis (150 aa), FASTA score: (71.4% identity in 28 aa overlap).; Possible antitoxin MazE5 2195347 mazE5 885605 mazE5 Mycobacterium tuberculosis H37Rv Possible antitoxin MazE5 NP_216459.1 2194970 R 83332 CDS NP_216460.1 15609081 885884 complement(2195344..2195934) 1 NC_000962.3 Rv1944c, (MTCY09F9.20), len: 196 aa. Conserved protein, similar to C-terminal part of SCE20.29|AL136058|CAB65585.1 hypothetical protein from Streptomyces coelicolor (338 aa), blastp scores, Identities = 37/131 (28%), Positives = 51/131 (38%).; hypothetical protein 2195934 885884 Rv1944c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216460.1 2195344 R 83332 CDS NP_216461.1 15609082 885980 2195989..2197353 1 NC_000962.3 Rv1945, (MTCY09F9.19c), len: 454 aa. Member of Mycobacterium tuberculosis REP13E12 repeat family. Similar to several others, best with Rv1148c|Z95584|MTCI65.15 (482 aa), FASTA score: opt: 2954, E(): 0, (97.1% identity in 454 aa overlap). Contains possible helix-turn-helix motif at aa 74-95 (+2.90 SD).; hypothetical protein 2197353 885980 Rv1945 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216461.1 2195989 D 83332 CDS NP_216462.1 15609083 885979 complement(2197508..2197960) 1 NC_000962.3 Rv1946c, (MTCY09F9.18), len: 150 aa. Possible lppG,conserved lipoprotein, showing some similarity to Rv1943c|MTCY09F9.21 conserved hypothetical protein from Mycobacterium tuberculosis (125 aa), FASTA score: (71.4% identity in 28 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible lipoprotein 2197960 lppG 885979 lppG Mycobacterium tuberculosis H37Rv Possible lipoprotein NP_216462.1 2197508 R 83332 CDS NP_216463.1 15609084 885976 2198024..2198425 1 NC_000962.3 Rv1947, (MTCY09F9.17c), len: 133 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2198425 885976 Rv1947 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216463.1 2198024 D 83332 CDS NP_216464.1 15609085 885975 complement(2198714..2199064) 1 NC_000962.3 Rv1948c, (MTCY09F9.16), len: 116 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2199064 885975 Rv1948c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216464.1 2198714 R 83332 CDS NP_216465.1 15609086 885974 complement(2199075..>2200034) 1 NC_000962.3 Rv1949c, (MTCY09F9.15), len: 319 aa. Conserved hypothetical protein, partial ORF. Rv1949c and Rv1950c|MTCY09F9.14 are similar but frameshifted with respect to Rv2077c|MTCY49.16C|Q10685 hypothetical 33.3 kd protein (323 aa), FASTA scores: opt: 459, E(): 2.8e-16,(54.8% identity in 157 aa overlap). Cosmid sequence appears to be correct, genomic sequence is also frameshifted in Mycobacterium bovis strain AF2122/97. Similar to Mycobacterium tuberculosis hypothetical proteins: Rv2542,Rv2077c, Rv2797c, Rv0963c, etc.; hypothetical protein >2200034 885974 Rv1949c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216465.1 2199075 R 83332 CDS NP_216466.1 15609087 885973 complement(2199998..2200189) 1 NC_000962.3 Rv1950c, (MTCY09F9.14), len: 63 aa. Conserved hypothetical protein, partial ORF. Highly similar to N-terminus of Rv2077c|MTCY49.16C|Q10685 hypothetical 33.3 kDa protein (323 aa), FASTA scores: opt: 280, E(): 1.2 e-16, (71.7% identity in 53 aa overlap) but homology continues in different frame ie MTCY09F9.15, cosmid sequence appears to be correct, genomic sequence is also frameshifted in Mycobacterium bovis strain AF2122/97.; hypothetical protein 2200189 885973 Rv1950c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216466.1 2199998 R 83332 CDS NP_216467.1 15609088 885972 complement(2200190..2200486) 1 NC_000962.3 Rv1951c, (MTCY09F9.13), len: 98 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical protein Rv2541 (135 aa) (40.9% identity in 88 aa overlap).; hypothetical protein 2200486 885972 Rv1951c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216467.1 2200190 R 83332 CDS NP_216468.1 15609089 885971 2200726..2200941 1 NC_000962.3 Rv1952, (MTCY09F9.12c), len: 71 aa. Possible vapB14,antitoxin, part of toxin-antitoxin (TA) operon with Rv1953 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in M. tuberculosis e.g. Rv2601A. Some similarity to P55510|Y4JJ_RHISN putative plasmid stability protein (85 aa), FASTA scores: opt: 127, E(): 0.00096, (42.5% identity in 73 aa overlap).; Possible antitoxin VapB14 2200941 vapB14 885971 vapB14 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB14 NP_216468.1 2200726 D 83332 CDS NP_216469.1 15609090 885970 2200938..2201249 1 NC_000962.3 Rv1953, (MTCY09F9.11c), len: 103 aa. Possible vapC14, toxin, part of toxin-antitoxin (TA) operon with Rv1952, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to O33827 plasmid stability-like protein from Thiobacillus ferrooxidans (143 aa), FASTA scores: opt: 170, E(): 3.5e-06, (45.3% identity in 75 aa overlap).; Possible toxin VapC14 2201249 vapC14 885970 vapC14 Mycobacterium tuberculosis H37Rv Possible toxin VapC14 NP_216469.1 2200938 D 83332 CDS NP_216470.1 15609091 885967 complement(2201223..2201744) 1 NC_000962.3 Rv1954c, (MTCY09F9.10), len: 173 aa. Hypothetical unknown protein, end overlaps next ORF upstream, Rv1955 (MTCY09F9.09c).; Hypothetical protein 2201744 885967 Rv1954c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216470.1 2201223 R 83332 CDS YP_007410632.1 448816759 14515862 2201277..2201579 1 NC_000962.3 Rv1954A, len: 100 aa. Hypothetical unknown protein.; Hypothetical protein 2201579 14515862 Rv1954A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_007410632.1 2201277 D 83332 CDS NP_216471.2 448824770 885966 2201719..2202096 1 NC_000962.3 Rv1955, (MTCY09F9.09c), len: 125 aa. Possible higB,toxin, part of toxin-antitoxin (TA) operon with Rv1956 (See Pandey and Gerdes, 2005; Gupta, 2009). Start overlaps another ORF, Rv1954c (MTCY09F9.10). Start changed since first submission (-45 aa). Predicted to be an outer membrane protein (See Song et al., 2008). Upon expression in E. coli has been shown to function as an antitoxin against Rv1956 (PubMed: 19016878); It is not clear if these conflicting results are due to expression in a heterologous system; In various publications, both gene names higA and higB have been assigned to both Rv1955 and Rv1956; we have chosen to call Rv1955 higB after consulting the authors.; Possible toxin HigB 2202096 higB 885966 higB Mycobacterium tuberculosis H37Rv Possible toxin HigB NP_216471.2 2201719 D 83332 CDS NP_216472.1 15609093 885964 2202138..2202587 1 NC_000962.3 Rv1956, (MTCY09F9.08c), len: 149 aa. Possible higA,antitoxin, part of toxin-antitoxin (TA) operon with Rv1955 (See Pandey and Gerdes, 2005; Gupta, 2009). Possible transcriptional regulatory protein, contains probable helix-turn-helix motif at aa 52-73 (+4.78 SD). Upon expression in E.coli Rv1956 has been shown to function as a toxin inhibiting cell growth and colony formation that is neutralized by coexpression with Rv1955 (PubMed: 19016878); It is not clear if these conflicting results are due to expression in a heterologous system. The gene names higA and higB have been assigned to both Rv1955 and Rv1956; we have chosen to call Rv1956 higA after consulting the authors.; Possible antitoxin HigA 2202587 higA 885964 higA Mycobacterium tuberculosis H37Rv Possible antitoxin HigA NP_216472.1 2202138 D 83332 CDS NP_216473.1 15609094 885961 2202584..2203129 1 NC_000962.3 Rv1957, (MTCY09F9.07c), len: 181 aa. Hypothetical unknown protein.; Hypothetical protein 2203129 885961 Rv1957 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216473.1 2202584 D 83332 CDS NP_216474.1 15609095 885960 complement(2203018..2203632) 1 NC_000962.3 Rv1958c, (MTCY09F9.06), len: 204 aa. Hypothetical unknown protein, questionable ORF; Hypothetical protein 2203632 885960 Rv1958c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216474.1 2203018 R 83332 CDS NP_216475.1 15609096 885958 complement(2203681..2203977) 1 NC_000962.3 Rv1959c, (MTCY09F9.05), len: 98 aa. Possible parE1,toxin, part of toxin-antitoxin (TA) operon with Rv1960c (See Pandey and Gerdes, 2005), similar to other hypothetical plasmid proteins e.g. AL117189|YPCD1.08 from Yersinia pestis (99 aa), FASTA scores: opt: 162, E(): 7.3e-05, (33.0% identity in 91 aa overlap); also some similarity to E145339 hypothetical protein (103 aa), FASTA scores: opt: 142, E(): 0.0003, (33.0% identity in 91 aa overlap).; Possible toxin ParE1 2203977 parE1 885958 parE1 Mycobacterium tuberculosis H37Rv Possible toxin ParE1 NP_216475.1 2203681 R 83332 CDS NP_216476.1 15609097 885957 complement(2203974..2204225) 1 NC_000962.3 Rv1960c, (MTCY09F9.04), len: 83 aa. Possible parD1,antitoxin, part of toxin-antitoxin (TA) operon with Rv1959c (See Pandey and Gerdes, 2005), similar to O85269|AF102990|AF102990_51 hypothetical protein of Yersinia enterocolitica (80 aa), FASTA scores: opt: 149,E(): 0.00037, (42.1% identity in 57 aa overlap).; Possible antitoxin ParD1 2204225 parD1 885957 parD1 Mycobacterium tuberculosis H37Rv Possible antitoxin ParD1 NP_216476.1 2203974 R 83332 CDS NP_216477.1 15609098 885954 2204212..2204706 1 NC_000962.3 Rv1961, MTCY09F9.03c, len: 164 aa. Hypothetical unknown protein.; Hypothetical protein 2204706 885954 Rv1961 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216477.1 2204212 D 83332 CDS NP_216478.1 15609099 885952 complement(2204866..2205273) 1 NC_000962.3 Rv1962c, (MTCY09F9.02), len: 135 aa. Possible vapC35, toxin, part of toxin-antitoxin (TA) operon with Rv1962A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv3408|MTCY78.20c (133 aa) (36.2% identity in 138 aa overlap); and Rv3384c (130 aa) (43.1% identity in 130 aa overlap); Possible toxin VapC35 Contains PIN domain 2205273 vapC35 885952 vapC35 Mycobacterium tuberculosis H37Rv Possible toxin VapC35 Contains PIN domain NP_216478.1 2204866 R 83332 CDS YP_007410641.1 448816768 14515903 complement(2205277..2205549) 1 NC_000962.3 Rv1962A, len: 90 aa. Possible vapB35, antitoxin,part of toxin-antitoxin (TA) operon with Rv1962c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3385c, Rv3407, Rv0626; Possible antitoxin VapB35 2205549 vapB35 14515903 vapB35 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB35 YP_007410641.1 2205277 R 83332 CDS NP_216479.1 15609100 885950 complement(2205582..2206802) 1 NC_000962.3 Rv1963c, (MTV051.01c-MTCY09F9.01), len: 406 aa. Probable mce3R, negative transcriptional regulatory protein, TetR family (see citation below); similar to several transcriptional regulator e.g. AL049485|SC6A5.30 Streptomyces coelicolor cosmid 6 a (404 aa), FASTA scores: opt: 319, E(): 6.4e-13, (29.5% identity in 373 aa overlap); and Z84498|MTCY9F9_1 (259 aa), FASTA scores: opt: 208, E(): 1.6e-07, (100.0% identity in 32 aa overlap). Contains probable helix-turn-helix at aa 36-57 (+4.23 SD) and two tet-R family signatures.; Probable transcriptional repressor (probably TetR-family) Mce3R 2206802 mce3R 885950 mce3R Mycobacterium tuberculosis H37Rv Probable transcriptional repressor (probably TetR-family) Mce3R NP_216479.1 2205582 R 83332 CDS NP_216480.1 15609101 885948 2207700..2208497 1 NC_000962.3 Rv1964, (MTV051.02), len: 265 aa. YrbE3A,hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa),O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa),Rv3501c|MTV023.08c|yrbE4A (254 aa), etc. Also highly similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. AAD24544.1|AF116213|YrbE1A from Mycobacterium leprae (112 aa); P45392|YRBE_ECOLI from Escherichia coli (260 aa), FASTA scores: opt: 893, E(): 0,(51.4% identity in 253 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002).; Conserved hypothetical integral membrane protein YrbE3A 2208497 yrbE3A 885948 yrbE3A Mycobacterium tuberculosis H37Rv Conserved hypothetical integral membrane protein YrbE3A NP_216480.1 2207700 D 83332 CDS NP_216481.1 15609102 885947 2208507..2209322 1 NC_000962.3 Rv1965, (MTV051.03), len: 271 aa. YrbE4B,hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa), FASTA scores: opt: 937, E(): 0, (54.3% identity in 254 aa overlap); O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. AAD24545.1|AF116213|YrbE1B from Mycobacterium leprae (106 aa); P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 218, E(): 1.2e-07, (24.1% identity in 245 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002).; Conserved hypothetical integral membrane protein YrbE3B 2209322 yrbE3B 885947 yrbE3B Mycobacterium tuberculosis H37Rv Conserved hypothetical integral membrane protein YrbE3B NP_216481.1 2208507 D 83332 CDS YP_177852.1 57116931 885944 2209327..2210604 1 NC_000962.3 Rv1966, (MTV051.04), len: 425 aa. Mce3A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. Note that previously known as mce3. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al.,2008).; Mce-family protein Mce3A 2210604 mce3A 885944 mce3A Mycobacterium tuberculosis H37Rv Mce-family protein Mce3A YP_177852.1 2209327 D 83332 CDS NP_216483.1 15609104 885943 2210601..2211629 1 NC_000962.3 Rv1967, (MTV051.05), len: 342 aa. Mce3B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); etc. Also similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al.,2008).; Mce-family protein Mce3B 2211629 mce3B 885943 mce3B Mycobacterium tuberculosis H37Rv Mce-family protein Mce3B NP_216483.1 2210601 D 83332 CDS NP_216484.1 15609105 885942 2211626..2212858 1 NC_000962.3 Rv1968, (MTV051.06), len: 410 aa. Mce3C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); etc. Also similar to others e.g. CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al.,2008).; Mce-family protein Mce3C 2212858 mce3C 885942 mce3C Mycobacterium tuberculosis H37Rv Mce-family protein Mce3C NP_216484.1 2211626 D 83332 CDS NP_216485.1 15609106 885940 2212855..2214126 1 NC_000962.3 Rv1969, (MTV051.07), len: 423 aa. Mce3D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al.,2008).; Mce-family protein Mce3D 2214126 mce3D 885940 mce3D Mycobacterium tuberculosis H37Rv Mce-family protein Mce3D NP_216485.1 2212855 D 83332 CDS NP_216486.1 15609107 885939 2214123..2215256 1 NC_000962.3 Rv1970, (MTV051.08), len: 377 aa. Possible lprM (alternate gene name: mce3E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains possible N-terminal signal sequence or membrane anchor and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002).; Possible Mce-family lipoprotein LprM (Mce-family lipoprotein Mce3E) 2215256 lprM 885939 lprM Mycobacterium tuberculosis H37Rv Possible Mce-family lipoprotein LprM (Mce-family lipoprotein Mce3E) NP_216486.1 2214123 D 83332 CDS NP_216487.1 15609108 885938 2215257..2216570 1 NC_000962.3 Rv1971, (MTV051.09), len: 437 aa. Mce3F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa), O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al.,2008).; Mce-family protein Mce3F 2216570 mce3F 885938 mce3F Mycobacterium tuberculosis H37Rv Mce-family protein Mce3F NP_216487.1 2215257 D 83332 CDS NP_216488.1 15609109 885937 2216592..2217167 1 NC_000962.3 Rv1972, (MTV051.10), len: 191 aa. Probable conserved Mce-associated membrane protein. Probably part of mce3 operon. Similar to several Mycobacterium tuberculosis proteins e.g. Rv1363c|Z75555|MTCY02B10.27C (261 aa), FASTA scores: opt: 342, E(): 1.2e-15, (31.8% identity in 195 aa overlap); Rv1362c, Rv0177 (near Mce operon 1), etc. Has hydrophobic stretch at aa 20-40.; Probable conserved Mce associated membrane protein 2217167 885937 Rv1972 Mycobacterium tuberculosis H37Rv Probable conserved Mce associated membrane protein NP_216488.1 2216592 D 83332 CDS NP_216489.1 15609110 885936 2217164..2217646 1 NC_000962.3 Rv1973, (MTV051.11), len: 160 aa. Possible conserved Mce-associated membrane protein. Probably part of mce3 operon. Similar to several other proteins from Mycobacterium tuberculosis e.g. Rv1362c|Z75555|MTCY02B10.26C (220 aa), FASTA scores: opt: 378, E(): 2.8e-19, (50.0% identity in 128 aa overlap); Rv1363c; Rv0177 (near Mce operon 1); etc. Contains possible N-terminal signal sequence or membrane anchor. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved Mce associated membrane protein 2217646 885936 Rv1973 Mycobacterium tuberculosis H37Rv Possible conserved Mce associated membrane protein NP_216489.1 2217164 D 83332 CDS NP_216490.1 15609111 885935 2217659..2218036 1 NC_000962.3 Rv1974, (MTV051.12), len: 125 aa. Probable conserved membrane protein, weakly similar to other Mycobacterium tuberculosis proteins e.g. Rv1271c|Z77137|MTCY50.11 (113 aa), FASTA scores: opt: 98, E(): 1.4, (24.5% identity in 110 aa overlap); Rv1804c; Rv1690. Has possible signal peptide or transmembrane stretch from aa 12-30. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein 2218036 885935 Rv1974 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_216490.1 2217659 D 83332 CDS NP_216491.1 15609112 885934 2218052..2218717 1 NC_000962.3 Rv1975, (MTV051.13), len: 221 aa. Conserved hypothetical protein, showing some similarity to AJ251435 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (193 aa). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 2218717 885934 Rv1975 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216491.1 2218052 D 83332 CDS NP_216492.1 15609113 885933 complement(2218844..2219251) 1 NC_000962.3 Rv1976c, (MTV051.14), len: 135 aa. Conserved hypothetical protein, similar to SC1C3.03c|AL023702 hypothetical protein from Streptomyces coelicolor (125 aa),FASTA score: opt: 223, E(): 3.3e-08, (39.6% identity in 111 aa overlap).; hypothetical protein 2219251 885933 Rv1976c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216492.1 2218844 R 83332 CDS NP_216493.1 15609114 885931 2219754..2220800 1 NC_000962.3 Rv1977, (MTV051.15), len: 348 aa. Conserved protein,similar to SCC123.20|AL136518 hypothetical protein from Streptomyces coelicolor (402 aa), blastp scores: Score = 311 bits (789), Expect = 5e-84 Identities = 156/316 (49%),Positives = 212/316 (66%); and PCC6803|D90907_31 Synechocystis sp. (303 aa), FASTA scores: opt: 533, E(): 4.7e- 29, (38.5% identity in 275 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Alternative nucleotide at position 2219929 (T->C; L59P) has been observed.; hypothetical protein 2220800 885931 Rv1977 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216493.1 2219754 D 83332 CDS NP_216494.1 15609115 885928 2220908..2221756 1 NC_000962.3 Rv1978, (MTV051.16), len: 282 aa. Conserved protein,similar to several hypothetical proteins and methyltransferases e.g. X86780|SHGCPIR.15 methyltransferase from S. hygroscopicus (211 aa), FASTA scores: opt: 151,E(): 0.0072, (30.6% identity in 121 aa overlap).; hypothetical protein 2221756 885928 Rv1978 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216494.1 2220908 D 83332 CDS NP_216495.2 448824771 885819 complement(2221719..2223164) 1 NC_000962.3 Rv1979c, (MTCY39.40-MTV051.17c), len: 481 aa. Possible permease, APC family possibly involved in transport of amino acid, showing some similarity to other permeases. Also similar to MTCY39.19 from Mycobacterium tuberculosis (28.2% identity in 277 aa overlap). Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Nucleotide position 2221796 in the genome sequence has been corrected, C:T resulting in V457I.; Possible conserved permease 2223164 885819 Rv1979c Mycobacterium tuberculosis H37Rv Possible conserved permease NP_216495.2 2221719 R 83332 CDS NP_216496.1 15609117 885925 complement(2223343..2224029) 1 NC_000962.3 Rv1980c, (MT2032, MTCY39.39), len: 228 aa. Mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below),identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al.,2008).; Immunogenic protein Mpt64 (antigen Mpt64/MPB64) 2224029 mpt64 885925 mpt64 Mycobacterium tuberculosis H37Rv Immunogenic protein Mpt64 (antigen Mpt64/MPB64) NP_216496.1 2223343 R 83332 CDS YP_177853.1 57116932 885923 complement(2224220..2225188) 1 NC_000962.3 Rv1981c, (MTCY39.38), len: 322 aa. NrdF1,ribonucleoside-diphosphate reductase, beta chain (see citation below), highly similar to others e.g. RIR4_SALTY|P17424 ribonucleoside-diphosphate reductase (319 aa), FASTA scores: opt: 1402, E(): 0, (66.0% identity in 315 aa overlap); etc. Also similar to Rv3048c|MTV012.63c from Mycobacterium tuberculosis. Contains PS00368 Ribonucleotide reductase small subunit signature. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: binds 2 iron ions (by similarity). Note that previously known as nrdF.; Ribonucleoside-diphosphate reductase (beta chain) NrdF1 (ribonucleotide reductase small subunit) (R2F protein) 2225188 nrdF1 885923 nrdF1 Mycobacterium tuberculosis H37Rv Ribonucleoside-diphosphate reductase (beta chain) NrdF1 (ribonucleotide reductase small subunit) (R2F protein) YP_177853.1 2224220 R 83332 CDS NP_216498.1 15609119 885816 complement(2225413..2225832) 1 NC_000962.3 Rv1982c, (MTCY39.37), len: 139 aa. Possible vapC36,toxin, part of toxin-antitoxin (TA) operon with Rv1982A,contains PIN domain, see Arcus et al. 2005. belongs to the UPF0110 family. Similar to Rv0624|Z92772|MTY20H10.05 from Mycobacterium tuberculosis (131 aa), FASTA scores: opt: 288, E(): 4.1e-14, (40.2% identity in 127 aa overlap); also similar to Rv0624, Rv2759c, and Rv0609; Possible toxin VapC36 Contains PIN domain 2225832 vapC36 885816 vapC36 Mycobacterium tuberculosis H37Rv Possible toxin VapC36 Contains PIN domain NP_216498.1 2225413 R 83332 CDS YP_004837054.1 345462040 11117810 complement(2225841..2226101) 1 NC_000962.3 Rv1982A, len: 86 aa. Possible vapB36, antitoxin,part of toxin-antitoxin (TA) operon with Rv1982c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv0623, Rv2760c, Rv0608; Possible antitoxin VapB36 2226101 vapB36 11117810 vapB36 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB36 YP_004837054.1 2225841 R 83332 CDS YP_177854.1 57116933 885921 2226244..2227920 1 NC_000962.3 Rv1983, (MTCY39.36c), len: 558 aa. PE_PGRS35, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to other PE proteins e.g. Rv0977, etc. Contains PS00141 Eukaryotic and viral aspartyl proteases active site.; PE-PGRS family protein PE_PGRS35 2227920 PE_PGRS35 885921 PE_PGRS35 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS35 YP_177854.1 2226244 D 83332 CDS NP_216500.1 15609121 885813 complement(2227908..2228561) 1 NC_000962.3 Rv1984c, (MTCY39.35), len: 217 aa. Cfp21, probable cutinase precursor with N-terminal signal sequence, similar to P41744|CUTI_ALTBR cutinase precursor from Alternaria brassicicola (209 aa), FASTA scores: opt: 283, E(): 2.2e-11, (32.6% identity in 193 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Rv3452, Rv3451,Rv2301, Rv1758, Rv3724. Belongs to the cutinase family.; Probable cutinase precursor CFP21 2228561 cfp21 885813 cfp21 Mycobacterium tuberculosis H37Rv Probable cutinase precursor CFP21 NP_216500.1 2227908 R 83332 CDS NP_216501.1 15609122 885818 complement(2228991..2229902) 1 NC_000962.3 Rv1985c, (MTCY39.34), len: 303 aa. Probable transcriptional regulatory protein, LysR family member. Similar to many regulatory proteins, especially ICIA_ECOLI|P24194 chromosome initiation inhibitor from Escherichia coli (297 aa), FASTA scores: opt: 520, E(): 1.1e-28, (35.8% identity in 285 aa overlap); and P94632|LYSG_CORGL lysine export regulator protein (290 aa),FASTA scores: opt: 705, E(): 0, (42.7% identity in 288 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature. Also contains helix-turn-helix motif at aa 22-43,(+5.52 SD). Belongs to the LysR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably LysR-family) 2229902 885818 Rv1985c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably LysR-family) NP_216501.1 2228991 R 83332 CDS NP_216502.1 15609123 885635 2230011..2230610 1 NC_000962.3 Rv1986, (MTCY39.33c), len: 199 aa. Probable conserved integral membrane protein, LysE family possibly involved in transport of Lysine, similar to P11667|YGGA_ECOLI hypothetical 23.2 kDa protein in sbm-fba intergenic region (211 aa), FASTA scores: opt: 379, E(): 1.5e-19, (37.3% identity in 185 aa overlap); and Q11154|Rv0488 hypothetical 20.9 kDa protein from M. tuberculosis (201 aa), FASTA scores: opt: 784, E(): 0,(63.4% identity in 186 aa overlap). Belongs to the LYSE/YGGA family.; Probable conserved integral membrane protein 2230610 885635 Rv1986 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216502.1 2230011 D 83332 CDS NP_216503.1 15609124 885815 2231026..2231454 1 NC_000962.3 Rv1987, (MTCY39.32c), len: 142 aa. Possible chitinase, similar to several e.g. P36909|CHIT_STRLI chitinase c precursor (619 aa) FASTA scores, opt: 324, E(): 1.2e-14, (39.5% identity in 129 aa overlap).; Possible chitinase 2231454 885815 Rv1987 Mycobacterium tuberculosis H37Rv Possible chitinase NP_216503.1 2231026 D 83332 CDS NP_216504.1 15609125 885632 2231680..2232219 1 NC_000962.3 Rv1988, (MTCY39.31c), len: 179 aa. Probable erm(37),23S rRNA methyltransferase, similar to ERME_SACER|P07287 rrna adenine n-6-methyltransferase (370 aa), FASTA scores: opt: 259, E(): 2e-11, (35.1% identity in 171 aa overlap); contains PS00092 N-6 Adenine-specific DNA methylases signature. Also similar to Mycobacterium tuberculosis Rv1010 ksgA 16S rRNA dimethyltransferase. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable 23S rRNA methyltransferase Erm(37) 2232219 erm(37) 885632 erm(37) Mycobacterium tuberculosis H37Rv Probable 23S rRNA methyltransferase Erm(37) NP_216504.1 2231680 D 83332 CDS NP_216505.1 15609126 885810 complement(2232739..2233299) 1 NC_000962.3 Rv1989c, (MTCY39.30), len: 186 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2233299 885810 Rv1989c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216505.1 2232739 R 83332 CDS NP_216506.1 15609127 885422 complement(2233296..2233637) 1 NC_000962.3 Rv1990c, (MTCY39.29), len: 113 aa. Probable transcriptional regulatory protein, similar to Mycobacterium tuberculosis Rv3188|AL021646|MTV014.32 (115 aa), FASTA scores: opt: 184, E(): 8.2e-07, (28.4% identity in 109 aa overlap). Contains probable helix-turn-helix motif at aa 20-44 (+4.22 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable transcriptional regulatory protein 2233637 885422 Rv1990c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_216506.1 2233296 R 83332 CDS YP_177656.1 57116934 3205104 complement(2233881..2234216) 1 NC_000962.3 Rv1990A, len: 111 aa. Possible dehydrogenase (fragment), similar to N-terminal part of several dehydrogenases and hypothetical proteins, e.g. Rv2750|MTV002.15|AL008967 from Mycobacterium tuberculosis (272 aa), FASTA scores: opt: 151, E(): 0.0045, (47.45% identity in 78 aa overlap), but lacks C-terminal part. Maybe a pseudogene. Also similar to U17129|RSU17129_7 putative short-chain alcohol dehydrogenase from Rhodococcus erythropolis (275 aa), FASTA scores: opt: 142, E(): 0.018,(54.15% identity in 48 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible dehydrogenase (fragment) 2234216 3205104 Rv1990A Mycobacterium tuberculosis H37Rv Possible dehydrogenase (fragment) YP_177656.1 2233881 R 83332 CDS NP_216507.1 15609128 885634 complement(2234305..2234649) 1 NC_000962.3 Rv1991c, (MTCY39.28), len: 114 aa. MazF6, toxin,part of toxin-antitoxin (TA) operon with Rv1991A. Some similarity to P13976|PEMK_ECOLI pemk protein (133 aa),FASTA scores: opt: 113, E(): 0.043, (29.2% identity in 113 aa overlap); and P96622|YDCE protein from Bacillus subtilis (116 aa), FASTA scores: opt: 227, E(): 6.9e-09, (37.4% identity in 115 aa overlap). Also similar to Mycobacterium tuberculosis Rv2801c, and Rv0659c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Toxin MazF6 2234649 mazF6 885634 mazF6 Mycobacterium tuberculosis H37Rv Toxin MazF6 NP_216507.1 2234305 R 83332 CDS YP_007410673.1 448816800 14515890 complement(2234643..2234891) 1 NC_000962.3 Rv1991A, len: 82 aa. MazE6, antitoxin, part of toxin-antitoxin (TA) operon with Rv1991c. Similar to ChpI of L. interrogans, FASTA scores: opt: 134, E(): 0.024,29.762% identity (65.476% similar) in 84 aa overlap. Note that Pandey and Gerdes, 2005 predicts a different N-terminus, adding 10 amino acids.; Antitoxin MazE6 2234891 mazE6 14515890 mazE6 Mycobacterium tuberculosis H37Rv Antitoxin MazE6 YP_007410673.1 2234643 R 83332 CDS NP_216508.1 15609129 888914 complement(2234991..2237306) 1 NC_000962.3 Rv1992c, (MTCY39.27), len: 771 aa. Probable ctpG,metal cation-transporting P-type ATPase G (transmembrane protein), similar to others, especially cadmium-transporting ATPases, e.g. NP_244904.1|NC_002570 cadmium-transporting ATPase from Bacillus halodurans (707 aa); P30336|CADA_BACFI probable cadmium-transporting ATPase from Bacillus firmus (723 aa); BAB47609.1|AB037671 cadmium resistance protein B from Staphylococcus aureus (804 aa); 3121832|Q60048|CADA_LISMO probable cadmium-transporting ATPase from Listeria monocytogenes (707 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0969|MTCY10D7.05c|ctpV putative cation transporter P-type ATPase V (770 aa); Rv1469; Rv0092; etc. Contains PS00435 Peroxidases proximal heme-ligand signature and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB.; Probable metal cation transporter P-type ATPase G CtpG 2237306 ctpG 888914 ctpG Mycobacterium tuberculosis H37Rv Probable metal cation transporter P-type ATPase G CtpG NP_216508.1 2234991 R 83332 CDS NP_216509.1 15609130 885631 complement(2237303..2237575) 1 NC_000962.3 Rv1993c, (MTCY39.26), len: 90 aa. Conserved protein,very similar to Rv3269|Z92771|MTCY71.09 hypothetical protein from Mycobacterium tuberculosis (93 aa), FASTA results: opt: 309, E(): 3.2e-16, (63.3% identity in 79 aa overlap). Also similar to Rv0968 (98 aa) (51.1% identity in 94 aa overlap).; hypothetical protein 2237575 885631 Rv1993c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216509.1 2237303 R 83332 CDS NP_216510.1 15609131 888889 complement(2237628..2237984) 1 NC_000962.3 Rv1994c, (MTCY39.25), len: 118 aa. CmtR,transcriptional regulator (See Cavet et al., 2003). Similar to MERR_STRLI|P30346 probable mercury resistance operon repressor (125 aa), FASTA scores: opt: 199, E(): 3e-08,(36.3% identity in 102 aa overlap). Note that primer extension analysis revealed two transcriptional start sites (See Chauhan et al., 2009).; Metal sensor transcriptional regulator CmtR (ArsR-SmtB family) 2237984 cmtR 888889 cmtR Mycobacterium tuberculosis H37Rv Metal sensor transcriptional regulator CmtR (ArsR-SmtB family) NP_216510.1 2237628 R 83332 CDS NP_216511.1 15609132 888917 2238141..2238908 1 NC_000962.3 Rv1995, (MTCY39.24c), len: 255 aa. Unknown protein.; hypothetical protein 2238908 888917 Rv1995 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216511.1 2238141 D 83332 CDS NP_216512.1 15609133 888863 2239004..2239957 1 NC_000962.3 Rv1996, (MTCY39.23c), len: 317 aa. Universal stress protein family protein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv2005c|Q10851|YK05_MYCTU (295 aa), FASTA scores: opt: 775,E(): 0, (50.3% identity in 316 aa overlap); Rv2026c (294 aa) (47.9% identity in 311 aa overlap); and Rv2623, etc. Also similar to SCJ1.30c|AL109962 hypothetical protein from Streptomyces coelicolor (328 aa). Predicted possible vaccine candidate (See Zvi et al., 2008).; Universal stress protein family protein 2239957 888863 Rv1996 Mycobacterium tuberculosis H37Rv Universal stress protein family protein NP_216512.1 2239004 D 83332 CDS NP_216513.1 15609134 888867 2240159..2242876 1 NC_000962.3 Rv1997, (MTCY39.22c, MTCY39.21c), len: 905 aa. Probable ctpF, metal cation-transporting P-type ATPase F (transmembrane protein), highly similar to others e.g. NP_250120.1|NC_002516 probable cation-transporting P-type ATPase from Pseudomonas aeruginosa (902 aa); NP_441217.1|NC_000911 cation-transporting ATPase (E1-E2 ATPase) from Synechocystis sp. strain PCC 6803 (905 aa); NP_404093.1|NC_003143 putative cation-transporting P-type ATPase from Yersinia pestis (908 aa); P37367|ATA1_SYNY3 cation-transporting ATPase pma1 from Synechocystis sp. (915 aa), FASTA scores: opt: 2392, E(): 0, (46.5% identity in 852 aa overlap); etc. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. Was frame-shifted in original cosmid sequence.; Probable metal cation transporter P-type ATPase A CtpF 2242876 ctpF 888867 ctpF Mycobacterium tuberculosis H37Rv Probable metal cation transporter P-type ATPase A CtpF NP_216513.1 2240159 D 83332 CDS NP_216514.1 15609135 888853 complement(2242945..2243721) 1 NC_000962.3 Rv1998c, (MTCY39.20), len: 258 aa. Conserved protein, showing some similarity with other hypothetical proteins e.g. U82823|SEU82823.03 Saccharopolyspora erythraea (266 aa), FASTA results: opt: 654, E(): 0, (43.8% identity in 249 aa overlap); and AL034446|SC1A9.07 Streptomyces coelicolor (251 aa), FASTA scores: opt: 592,E(): 1.5e-31, (43.4% identity in 251 aa overlap).; hypothetical protein 2243721 888853 Rv1998c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216514.1 2242945 R 83332 CDS NP_216515.1 15609136 888881 complement(2243816..2245138) 1 NC_000962.3 Rv1999c, (MTCY39.19), len: 440 aa. Probable conserved integral membrane protein, possibly transporter of cationic amino acid, similar to many transporters,especially amino acid transporters, e.g. CAC08265.1|AL392146 putative amino acid transporter from Streptomyces coelicolor (414 aa); P39277|YJEH_ECOLI hypothetical 44.8 kDa protein from Escherichia coli (418 aa), FASTA scores, opt: 343, E(): 6.6e-15, (27.2% identity in 408 aa overlap); etc. Also similar to Rv1979c from Mycobacterium tuberculosis, FASTA score: (28.2% identity in 277 aa overlap); Rv2127, Rv0346c, Rv0522, etc. Seems to belong to the APC family.; Probable conserved integral membrane protein 2245138 888881 Rv1999c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216515.1 2243816 R 83332 CDS NP_216516.1 15609137 888864 2245209..2246822 1 NC_000962.3 Rv2000, (MTCY39.18c), len: 537 aa. Unknown protein.; hypothetical protein 2246822 888864 Rv2000 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216516.1 2245209 D 83332 CDS NP_216517.1 15609138 888880 2246832..2247584 1 NC_000962.3 Rv2001, (MTCY39.17c), len: 250 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis Rv0466.; hypothetical protein 2247584 888880 Rv2001 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216517.1 2246832 D 83332 CDS NP_216518.1 15609139 888857 2247660..2248442 1 NC_000962.3 Rv2002, (MTCY39.16c), len: 260 aa. FabG3,20-beta-hydroxysteroid dehydrogenase. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase) 2248442 fabG3 888857 fabG3 Mycobacterium tuberculosis H37Rv Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase) NP_216518.1 2247660 D 83332 CDS NP_216519.1 15609140 888818 complement(2248563..2249420) 1 NC_000962.3 Rv2003c, (MTCY39.14), len: 285 aa. Conserved hypothetical protein.; hypothetical protein 2249420 888818 Rv2003c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216519.1 2248563 R 83332 CDS NP_216520.1 15609141 888817 complement(2249478..2250974) 1 NC_000962.3 Rv2004c, (MTCY39.13), len: 498 aa. Conserved protein. Contains PS00017 ATP/GTP-binding site motif A.; hypothetical protein 2250974 888817 Rv2004c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216520.1 2249478 R 83332 CDS NP_216521.1 15609142 888831 complement(2250996..2251883) 1 NC_000962.3 Rv2005c, (MTCY39.12), len: 295 aa. Universal stress protein family protein. Predicted possible vaccine candidate (See Zvi et al., 2008).; Universal stress protein family protein 2251883 888831 Rv2005c Mycobacterium tuberculosis H37Rv Universal stress protein family protein NP_216521.1 2250996 R 83332 CDS YP_177855.1 57116935 888943 2252002..2255985 1 NC_000962.3 Rv2006, (MTCY39.11c), len: 1327 aa. OtsB1,trehalose-6-phosphate phosphatase (see citations below). Belongs to Glycosyl hydrolases family 65. Note that previously known as otsB. Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable trehalose-6-phosphate phosphatase OtsB1 (trehalose-phosphatase) (TPP) 2255985 otsB1 888943 otsB1 Mycobacterium tuberculosis H37Rv Probable trehalose-6-phosphate phosphatase OtsB1 (trehalose-phosphatase) (TPP) YP_177855.1 2252002 D 83332 CDS NP_216523.1 15609144 888887 complement(2256084..2256428) 1 NC_000962.3 Rv2007c, (MTCY39.10), len: 114 aa. FdxA, ferredoxin,similar to many e.g. FER_MYCSM P00215 ferredoxin,Mycobacterium smegmatis (106 aa).; Ferredoxin FdxA 2256428 fdxA 888887 fdxA Mycobacterium tuberculosis H37Rv Ferredoxin FdxA NP_216523.1 2256084 R 83332 CDS NP_216524.1 15609145 888813 complement(2256617..2257942) 1 NC_000962.3 Rv2008c, (MTCY39.09), len: 441 aa. Conserved hypothetical protein. Contains PS00017 ATP/GTP-binding site motif A, PS00501 Signal peptidases I serine active site. Also contains helix-turn-helix motif at aa 258-279.; hypothetical protein 2257942 888813 Rv2008c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216524.1 2256617 R 83332 CDS NP_216525.1 15609146 888925 2258030..2258272 1 NC_000962.3 Rv2009, (MTCY39.08c), len: 80 aa. VapB15, antitoxin,part of toxin-antitoxin (TA) operon with Rv2010 (See Arcus et al., 2005; Pandey and Gerdes, 2005).; Antitoxin VapB15 2258272 vapB15 888925 vapB15 Mycobacterium tuberculosis H37Rv Antitoxin VapB15 NP_216525.1 2258030 D 83332 CDS NP_216526.1 15609147 888933 2258273..2258671 1 NC_000962.3 Rv2010, (MTCY39.07c), len: 132 aa. VapC15, toxin,part of toxin-antitoxin (TA) operon with Rv2009, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes,2005).; Toxin VapC15 2258671 vapC15 888933 vapC15 Mycobacterium tuberculosis H37Rv Toxin VapC15 NP_216526.1 2258273 D 83332 CDS NP_216527.1 15609148 888922 complement(2258854..2259285) 1 NC_000962.3 Rv2011c, (MTCY39.06), len: 143 aa. Conserved hypothetical protein, probable transcription repressor. Contains IPR011991 Winged helix-turn-helix transcription repressor DNA-binding domain.; Conserved hypothetical protein, probable transcription repressor 2259285 888922 Rv2011c Mycobacterium tuberculosis H37Rv Conserved hypothetical protein, probable transcription repressor NP_216527.1 2258854 R 83332 CDS NP_216528.1 15609149 888927 2259326..2259820 1 NC_000962.3 Rv2012, (MTCY39.05c), len: 164 aa. Conserved hypothetical protein.; hypothetical protein 2259820 888927 Rv2012 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216528.1 2259326 D 83332 CDS NP_216529.2 57116936 887546 2260665..2261144 1 NC_000962.3 Rv2013, (MTCY39.04c), len: 159 aa. Transposase,shows similarity to N-terminal part of transposase and insertion element hypothetical proteins. Length changed since first submission (no clear start apparent).; Transposase 2261144 887546 Rv2013 Mycobacterium tuberculosis H37Rv Transposase NP_216529.2 2260665 D 83332 CDS NP_216530.2 57116937 887547 2261098..2261688 1 NC_000962.3 Rv2014, (MTCY39.03c), len: 196 aa. Transposase,similar to insertion elements; possibly made by frameshifting with respect to Rv2013. Length changed since first submission.; Transposase 2261688 887547 Rv2014 Mycobacterium tuberculosis H37Rv Transposase NP_216530.2 2261098 D 83332 CDS NP_216531.1 15609152 888378 complement(2261816..2263072) 1 NC_000962.3 Rv2015c, (MTV018.02c), len: 418 aa. Conserved hypothetical protein. Nearly identical to Mycobacterium tuberculosis Rv1765c|MTCY28.31c, (378 aa), an ORF starting next to ISB9, and ending in IS6110. Different N-terminus chosen and C-terminus differs as that of Rv1765c has been truncated by IS6110. Does not show similarities with transposases. Contains IPR002711 HNH endonuclease,IPR003615 HNH nuclease, IPR003870 DUF222 domains.; hypothetical protein 2263072 888378 Rv2015c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216531.1 2261816 R 83332 CDS NP_216532.1 15609153 888630 2263426..2264001 1 NC_000962.3 Rv2016, (MTV018.03), len: 191 aa. Hypothetical protein.; Hypothetical protein 2264001 888630 Rv2016 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216532.1 2263426 D 83332 CDS NP_216533.1 15609154 888435 2263998..2265038 1 NC_000962.3 Rv2017, (MTV018.04), len: 346 aa. Transcriptional regulator. Contains PS00142 Neutral zinc metallopeptidases,zinc-binding region signature in C-terminal half, may be fortuitous. Contains probable helix-turn-helix motif at aa 18-39 (Score 2243, +6.83 SD); IPR001387 Helix-turn-helix type 3.; Transcriptional regulatory protein 2265038 888435 Rv2017 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein NP_216533.1 2263998 D 83332 CDS NP_216534.1 15609155 887560 2265280..2265999 1 NC_000962.3 Rv2018, (MTV018.05), len: 239 aa. Conserved protein. Contains probable helix-turn-helix motif at aa 215-236 (Score 1175, +3.19 SD).; hypothetical protein 2265999 887560 Rv2018 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216534.1 2265280 D 83332 CDS NP_216535.1 15609156 887665 2265989..2266405 1 NC_000962.3 Rv2019, (MTV018.06), len: 138 aa. Conserved protein.; hypothetical protein 2266405 887665 Rv2019 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216535.1 2265989 D 83332 CDS NP_216536.1 15609157 887656 complement(2266421..2266720) 1 NC_000962.3 Rv2020c, (MTV018.07c), len: 99 aa. Conserved hypothetical protein, nearly identical to C-terminal part of hypothetical protein RvD1-Rv2024c' from Mycobacterium bovis BCG (1606 aa) emb|CAB44655.1| (Y18605). Corresponds to deletion region RvD1 so probably truncated protein.; hypothetical protein 2266720 887656 Rv2020c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216536.1 2266421 R 83332 CDS NP_216537.1 15609158 888092 complement(2266805..2267110) 1 NC_000962.3 Rv2021c, (MTV018.08c), len: 101 aa. Regulatory protein, similar to many. Contains probable helix-turn-helix at aa 45-66 (Score 1472, +4.20 SD); IPR001387 Helix-turn-helix type 3 domain.; Transcriptional regulatory protein 2267110 888092 Rv2021c Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein NP_216537.1 2266805 R 83332 CDS NP_216538.1 15609159 888129 complement(2267119..2267724) 1 NC_000962.3 Rv2022c, (MTV018.09c), len: 201 aa. Conserved protein, similar to many.; hypothetical protein 2267724 888129 Rv2022c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216538.1 2267119 R 83332 CDS NP_216539.1 15609160 887444 complement(2267749..2268108) 1 NC_000962.3 Rv2023c, (MTV018.10c), len: 119 aa. Hypothetical protein, alternative upstream start possible.; Hypothetical protein 2268108 887444 Rv2023c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216539.1 2267749 R 83332 CDS NP_216540.1 15609161 888351 complement(2268693..2270240) 1 NC_000962.3 Rv2024c, (MTV018.11c), len: 515 aa. Conserved hypothetical protein. Identical to N-terminal part of much larger hypothetical protein, RvD1-Rv2024c' (1606 aa), from Mycobacterium bovis BCG: CAB44655.1|Y18605|13881753|AAK46361.1|AE007059 so probably truncated. Part of RvD1 chromosomal deletion region.; hypothetical protein 2270240 888351 Rv2024c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216540.1 2268693 R 83332 CDS NP_216541.1 15609162 888782 complement(2270750..2271748) 1 NC_000962.3 Rv2025c, (MTV018.12c), len: 332 aa. Conserved transmembrane protein, involved in transport of metal ions,contains IPR002524 Cation efflux protein domain.; hypothetical protein 2271748 888782 Rv2025c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216541.1 2270750 R 83332 CDS NP_216542.1 15609163 887460 complement(2271863..2272747) 1 NC_000962.3 Rv2026c, (MTV018.13c), len: 294 aa. Universal stress protein family protein, contains IPR006016 UspA domain.; Universal stress protein family protein 2272747 887460 Rv2026c Mycobacterium tuberculosis H37Rv Universal stress protein family protein NP_216542.1 2271863 R 83332 CDS NP_216543.1 15609164 888471 complement(2272787..2274508) 1 NC_000962.3 Rv2027c, (MTV018.14c), len: 573 aa. DosT, Histidine kinase response regulator, highly similar to others.; Two component sensor histidine kinase DosT 2274508 dosT 888471 dosT Mycobacterium tuberculosis H37Rv Two component sensor histidine kinase DosT NP_216543.1 2272787 R 83332 CDS NP_216544.1 15609165 888494 complement(2274569..2275408) 1 NC_000962.3 Rv2028c, (MTV018.15c), len: 279 aa. Universal stress protein family protein, highly similar to many, contains IPR006016 UspA domain.; Universal stress protein family protein 2275408 888494 Rv2028c Mycobacterium tuberculosis H37Rv Universal stress protein family protein NP_216544.1 2274569 R 83332 CDS NP_216545.1 15609166 887491 complement(2275405..2276424) 1 NC_000962.3 Rv2029c, (MTV018.16c), len: 339 aa. PfkB,phosphofructokinase. Contains PS00583 pfkB family of carbohydrate kinases signature 1. Predicted possible vaccine candidate (See Zvi et al., 2008).; 6-phosphofructokinase PfkB (phosphohexokinase) (phosphofructokinase) 2276424 pfkB 887491 pfkB Mycobacterium tuberculosis H37Rv 6-phosphofructokinase PfkB (phosphohexokinase) (phosphofructokinase) NP_216545.1 2275405 R 83332 CDS NP_216546.1 15609167 887536 complement(2276441..2278486) 1 NC_000962.3 Rv2030c, (MTV018.17c), len: 681 aa. Conserved protein. Predicted possible vaccine candidate (See Zvi et al., 2008).; hypothetical protein 2278486 887536 Rv2030c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216546.1 2276441 R 83332 CDS NP_216547.1 15609168 887579 complement(2278498..2278932) 1 NC_000962.3 Rv2031c, (MTV018.18c), len: 144 aa. HspX, heat shock protein localized in the inner membrane (see citations below). Identical to P30223|14KD_MYCTU 14 KD antigen (16 kDa antigen) (HSP 16.3) of Mycobacterium tuberculosis (143 aa). Belongs to the small heat shock protein (HSP20) family. Also known as alpha-crystallin and gene as acr (see some citations below). Predicted possible vaccine candidate (See Zvi et al., 2008).; Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3) 2278932 hspX 887579 hspX Mycobacterium tuberculosis H37Rv Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3) NP_216547.1 2278498 R 83332 CDS NP_216548.1 15609169 887582 2279129..2280124 1 NC_000962.3 Rv2032, (MTV018.19), len: 331 aa. Acg (for acr-coregulated gene), conserved protein possibly member of a superfamily of classical nitroreductases (see Purkayastha et al., 2002), similar to Rv3127 and Rv3131. Predicted possible vaccine candidate (See Zvi et al., 2008).; Conserved protein Acg 2280124 acg 887582 acg Mycobacterium tuberculosis H37Rv Conserved protein Acg NP_216548.1 2279129 D 83332 CDS NP_216549.1 15609170 887972 complement(2280240..2281082) 1 NC_000962.3 Rv2033c, (MTV018.20), len: 280 aa. Conserved hypothetical protein, similar to many.; hypothetical protein 2281082 887972 Rv2033c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216549.1 2280240 R 83332 CDS NP_216550.1 15609171 887859 2281294..2281617 1 NC_000962.3 Rv2034, (MTV018.21), len: 107 aa. Repressor protein belonging to the ArsR family. Contains probable helix-turn-helix at aa 32-53 (S core 1350, +3.78 SD).; ArsR repressor protein 2281617 887859 Rv2034 Mycobacterium tuberculosis H37Rv ArsR repressor protein NP_216550.1 2281294 D 83332 CDS NP_216551.1 15609172 887445 2281614..2282102 1 NC_000962.3 Rv2035, (MTV018.22), len: 162 aa. Conserved hypothetical protein, similar to many. Contains IPR013538 Activator of Hsp90 ATPase homologue 1-like.; hypothetical protein 2282102 887445 Rv2035 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216551.1 2281614 D 83332 CDS NP_216552.1 15609173 887433 2282099..2282740 1 NC_000962.3 Rv2036, (MTV018.23), len: 213 aa. Conserved hypothetical protein; similar to many.; hypothetical protein 2282740 887433 Rv2036 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216552.1 2282099 D 83332 CDS NP_216553.1 15609174 888021 complement(2282747..2283721) 1 NC_000962.3 Rv2037c, (MTV018.24c), len: 324 aa. Conserved transmembrane protein, similar to many. Alternative nucleotide at position 2282787 (C->T; C312Y) has been observed. Contains IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase motif.; Conserved transmembrane protein 2283721 888021 Rv2037c Mycobacterium tuberculosis H37Rv Conserved transmembrane protein NP_216553.1 2282747 R 83332 CDS NP_216554.1 15609175 887998 complement(2283723..2284796) 1 NC_000962.3 Rv2038c, (MTV018.25c), len: 357 aa. Probable sugar-transport ATP-binding protein ABC transporter (see citation below), similar to many. Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop).; Probable sugar-transport ATP-binding protein ABC transporter 2284796 887998 Rv2038c Mycobacterium tuberculosis H37Rv Probable sugar-transport ATP-binding protein ABC transporter NP_216554.1 2283723 R 83332 CDS NP_216555.1 15609176 887729 complement(2284799..2285641) 1 NC_000962.3 Rv2039c, (MTV018.26c), len: 280 aa. Probable sugar-transport integral membrane protein ABC transporter (see citation below), similar to many. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. Also contains possible helix-turn-helix motif at aa 171-192, although this is probably fortuitous.; Probable sugar-transport integral membrane protein ABC transporter 2285641 887729 Rv2039c Mycobacterium tuberculosis H37Rv Probable sugar-transport integral membrane protein ABC transporter NP_216555.1 2284799 R 83332 CDS NP_216556.1 15609177 887893 complement(2285628..2286530) 1 NC_000962.3 Rv2040c, (MTV018.27c), len: 300 aa. Probable sugar-transport integral membrane protein ABC transporter (see citation below), similar to many.; Probable sugar-transport integral membrane protein ABC transporter 2286530 887893 Rv2040c Mycobacterium tuberculosis H37Rv Probable sugar-transport integral membrane protein ABC transporter NP_216556.1 2285628 R 83332 CDS NP_216557.1 15609178 887474 complement(2286527..2287846) 1 NC_000962.3 Rv2041c, (MTV018.28c), len: 439 aa. Probable sugar-binding lipoprotein component of sugar transport system, similar to many. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable sugar-binding lipoprotein 2287846 887474 Rv2041c Mycobacterium tuberculosis H37Rv Probable sugar-binding lipoprotein NP_216557.1 2286527 R 83332 CDS NP_216558.1 15609179 887497 complement(2287884..2288681) 1 NC_000962.3 Rv2042c, (MTV018.29c), len: 265 aa. Conserved protein, similar to many.; hypothetical protein 2288681 887497 Rv2042c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216558.1 2287884 R 83332 CDS NP_216559.1 15609180 888260 complement(2288681..2289241) 1 NC_000962.3 Rv2043c, (MTV018.30c), len: 186 aa. PncA,pyrazinamidase/nicotinamidase (see citations below),involved in susceptibility or resistance to antituberculous drug pyrazinamide.; Pyrazinamidase/nicotinamidase PncA (PZase) 2289241 pncA 888260 pncA Mycobacterium tuberculosis H37Rv Pyrazinamidase/nicotinamidase PncA (PZase) NP_216559.1 2288681 R 83332 CDS NP_216560.1 15609181 888243 complement(2289282..2289599) 1 NC_000962.3 Rv2044c, (MTV018.31c), len: 105 aa. Conserved hypothetical protein, similar to many.; hypothetical protein 2289599 888243 Rv2044c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216560.1 2289282 R 83332 CDS NP_216561.1 15609182 888358 complement(2289685..2291220) 1 NC_000962.3 Rv2045c, (MTV018.32c), len: 511 aa. LipT,carboxylesterase, similar to many. Contains PS00941 Carboxylesterases type-B signature 2. Contains PS00122 Carboxylesterases type-B serine active site.; Carboxylesterase LipT 2291220 lipT 888358 lipT Mycobacterium tuberculosis H37Rv Carboxylesterase LipT NP_216561.1 2289685 R 83332 CDS NP_216562.1 15609183 888308 2291269..2291925 1 NC_000962.3 Rv2046, (MTV018.33), len: 218 aa. Probable lppI,lipoprotein contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LppI 2291925 lppI 888308 lppI Mycobacterium tuberculosis H37Rv Probable lipoprotein LppI NP_216562.1 2291269 D 83332 CDS NP_216563.1 15609184 888529 complement(2291962..2294526) 1 NC_000962.3 Rv2047c, (MTV018.34c), len: 854 aa. Conserved hypothetical protein, similar to many. Contains IPR016040 NAD(P)-binding domain at N-terminus.; hypothetical protein 2294526 888529 Rv2047c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216563.1 2291962 R 83332 CDS NP_216564.2 448824772 888350 complement(2294531..2306986) 1 NC_000962.3 Rv2048c, (MTV018.35c), len: 4151 aa. Pks12,polyketide synthase similar to many. Contains 2x PS00012 Phosphopantetheine attachment site, 2x PS00606 Beta-ketoacyl synthases active site, and PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2297976 in the genome sequence has been corrected, G:A resulting in S3004L.; Polyketide synthase Pks12 2306986 pks12 888350 pks12 Mycobacterium tuberculosis H37Rv Polyketide synthase Pks12 NP_216564.2 2294531 R 83332 CDS NP_216565.1 15609186 888593 complement(2307293..2307517) 1 NC_000962.3 Rv2049c, (MTV018.36c), len: 74 aa. Conserved hypothetical protein.; hypothetical protein 2307517 888593 Rv2049c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216565.1 2307293 R 83332 CDS NP_216566.1 15609187 888598 2307821..2308156 1 NC_000962.3 Rv2050, (MTV018.37), len: 111 aa. Conserved protein,similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2308156 888598 Rv2050 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216566.1 2307821 D 83332 CDS NP_216567.1 15609188 887402 complement(2308131..2310755) 1 NC_000962.3 Rv2051c, (MTV018.38c), len: 874 aa. Ppm1,Polyprenol-monophosphomannose synthase. Transfers mannose from GDP-Mannose to all endogenous polyprenol-phosphates in Mycobacterium tuberculosis, proven experimentally (A. Baulard, Institut Pasteur de Lille: see citation below).; Polyprenol-monophosphomannose synthase Ppm1 2310755 ppm1 887402 ppm1 Mycobacterium tuberculosis H37Rv Polyprenol-monophosphomannose synthase Ppm1 NP_216567.1 2308131 R 83332 CDS NP_216568.1 15609189 888608 complement(2310913..2312517) 1 NC_000962.3 Rv2052c, (MTV018.39c), len: 534 aa. Conserved protein, similar to many. Contains IPR013108 Amidohydrolase 3 domain.; hypothetical protein 2312517 888608 Rv2052c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216568.1 2310913 R 83332 CDS NP_216569.1 15609190 888768 complement(2312522..2313049) 1 NC_000962.3 Rv2053c, (MTV018.40c-MTCY63A.07), len: 175 aa. Probable fxsA, transmembrane protein. Contains IPR007313 FxsA cytoplasmic membrane protein domain in N-terminus; Probable transmembrane protein FxsA 2313049 fxsA 888768 fxsA Mycobacterium tuberculosis H37Rv Probable transmembrane protein FxsA NP_216569.1 2312522 R 83332 CDS NP_216570.1 15609191 888722 2313125..2313838 1 NC_000962.3 Rv2054, (MTCY63A.06c), len: 237 aa. Conserved protein, similar to many. Contains IPR002925 Dienelactone hydrolase domain.; hypothetical protein 2313838 888722 Rv2054 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216570.1 2313125 D 83332 CDS NP_216571.1 15609192 887817 complement(2314087..2314353) 1 NC_000962.3 Rv2055c, (MTCY63A.05), len: 88 aa. rpsR2, 30S ribosomal protein S18, similar to many. Also similar to rpsR|Rv0055|MTCY21D4.18 from Mycobacterium tuberculosis (50.0% identity in 84 aa overlap).; 30S ribosomal protein S18 RpsR2 2314353 rpsR2 887817 rpsR2 Mycobacterium tuberculosis H37Rv 30S ribosomal protein S18 RpsR2 NP_216571.1 2314087 R 83332 CDS NP_216572.1 15609193 887819 complement(2314354..2314659) 1 NC_000962.3 Rv2056c, (MTCY63A.04), len: 101 aa. rpsN2, 30S ribosomal protein S14, similar to many. Also similar to rpsN|Rv0717|MTCY210.36 from Mycobacterium tuberculosis,(50.0% identity in 62 aa overlap).; 30S ribosomal protein S14 RpsN2 2314659 rpsN2 887819 rpsN2 Mycobacterium tuberculosis H37Rv 30S ribosomal protein S14 RpsN2 NP_216572.1 2314354 R 83332 CDS YP_177856.1 57116938 887807 complement(2314661..2314825) 1 NC_000962.3 Rv2057c, (MTCY63A.03), len: 54 aa. rpmG1, 50S ribosomal protein L33, similar to many. Note that previously known as rpmG.; 50S ribosomal protein L33 RpmG1 2314825 rpmG1 887807 rpmG1 Mycobacterium tuberculosis H37Rv 50S ribosomal protein L33 RpmG1 YP_177856.1 2314661 R 83332 CDS NP_216574.1 15609195 887801 complement(2314825..2315061) 1 NC_000962.3 Rv2058c, (MTCY63A.02), len: 78 aa. rpmB2, 50S ribosomal protein L28, very similar to rL28 of M. tuberculosis. Also similar to rpmB (Rv0105c) of Mycobacterium tuberculosis.; 50S ribosomal protein L28 RpmB2 2315061 rpmB2 887801 rpmB2 Mycobacterium tuberculosis H37Rv 50S ribosomal protein L28 RpmB2 NP_216574.1 2314825 R 83332 CDS NP_216575.1 15609196 888402 2315174..2316709 1 NC_000962.3 Rv2059, (MTCY63A.01c), len: 511 aa. Conserved hypothetical protein. Some similarity to EWLA protein gp|U52850|ERU52850_1 Erysipelothrix rhusiopathiae 36 k (304 aa), FASTA score, opt: 287 E(): 6.9e-09; 27.2% identity in 228 aa overlap. There appears to be a frameshift in this ORF around position 3315980 that causes an overlap with next ORF. C-terminal end of protein may be wrong. No error can be found to account for this.; hypothetical protein 2316709 888402 Rv2059 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216575.1 2315174 D 83332 CDS NP_216576.1 15609197 888388 2316279..2316680 1 NC_000962.3 Rv2060, (MTV019.01), len: 133 aa. Possible conserved integral membrane protein smaller than but similar to several hypothetical bacterial proteins e.g. >emb|CAC29843.1| (AL583918) putative ABC-transporter transmembrane protein [Mycobacterium leprae] Length = 286 and P44691|YEBI_HAEIN (261 aa). FASTA scores: P44691|YEBI_HAEIN hypothetical protein HI0407 (261 aa) opt: 218, E(): 4.2e-08; 31.1% identity in 122 aa overlap. Maybe frameshift upstream at position 3315980 but no error can be found to account for this.; Possible conserved integral membrane protein 2316680 888388 Rv2060 Mycobacterium tuberculosis H37Rv Possible conserved integral membrane protein NP_216576.1 2316279 D 83332 CDS NP_216577.1 15609198 888246 complement(2316681..2317085) 1 NC_000962.3 Rv2061c, (MTV019.02c), len: 134 aa. Conserved protein. Similar to many. Contains IPR019965 F420-dependent enzyme, PPOX class, family Rv2061, domain. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2317085 888246 Rv2061c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216577.1 2316681 R 83332 CDS NP_216578.1 15609199 888252 complement(2317169..2320753) 1 NC_000962.3 Rv2062c, (MTCY49.01c, MTV019.03), len: 1194 aa. cobN, cobalamin biosynthesis protein, similar to many.; Cobalamin biosynthesis protein CobN 2320753 cobN 888252 cobN Mycobacterium tuberculosis H37Rv Cobalamin biosynthesis protein CobN NP_216578.1 2317169 R 83332 CDS YP_177657.1 57116939 3205116 2320831..2321064 1 NC_000962.3 Rv2063, len: 77 aa. MazE7, antitoxin, part of toxin-antitoxin (TA) operon with Rv2063A (See Pandey and Gerdes, 2005), similar to many. This ORF replaces previous Rv2063c on other strand.; Antitoxin MazE7 2321064 mazE7 3205116 mazE7 Mycobacterium tuberculosis H37Rv Antitoxin MazE7 YP_177657.1 2320831 D 83332 CDS YP_004837055.2 448824773 14515888 2321057..2321467 1 NC_000962.3 Rv2063A, len: 136 aa. Possible mazF7 toxin, part of toxin-antitoxin (TA) operon with Rv2063 (See Pandey and Gerdes, 2005).; Possible toxin MazF7 2321467 mazF7 14515888 mazF7 Mycobacterium tuberculosis H37Rv Possible toxin MazF7 YP_004837055.2 2321057 D 83332 CDS NP_216580.1 15609201 888140 2321451..2322542 1 NC_000962.3 Rv2064, (MTCY49.03), len: 363 aa. CobG, precorrin-3B synthase, cobalamin biosynthesis protein.; Precorrin-3B synthase CobG 2322542 cobG 888140 cobG Mycobacterium tuberculosis H37Rv Precorrin-3B synthase CobG NP_216580.1 2321451 D 83332 CDS NP_216581.1 15609202 888464 2322552..2323178 1 NC_000962.3 Rv2065, (MTCY49.04), len: 208 aa. CobH, precorrin-8X methylmutase (aka precorrin isomerase), similar to many.; Precorrin-8X methylmutase CobH (aka precorrin isomerase) 2323178 cobH 888464 cobH Mycobacterium tuberculosis H37Rv Precorrin-8X methylmutase CobH (aka precorrin isomerase) NP_216581.1 2322552 D 83332 CDS NP_216582.1 15609203 888483 2323175..2324701 1 NC_000962.3 Rv2066, (MTCY49.05), len: 508 aa. Probable CobI-CobJ fusion protein, S-adenosyl-L-methionine-precorrin-2 methyl transferase and precorrin-3 methylase. Similar in N-terminal half (aa 1-240) to many S-adenosyl-L-methionine-precorrin-2 methyl transferase (244 aa), and in C-terminal half (aa 240-508) to precorrin-3 methylase (254 aa).; Probable bifunctional protein, CobI-COBJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase 2324701 cobI 888483 cobI Mycobacterium tuberculosis H37Rv Probable bifunctional protein, CobI-COBJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase NP_216582.1 2323175 D 83332 CDS NP_216583.1 15609204 888752 complement(2324647..2325870) 1 NC_000962.3 Rv2067c, (MTCY49.06c), len: 407 aa. Conserved protein, similar to many.; hypothetical protein 2325870 888752 Rv2067c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216583.1 2324647 R 83332 CDS NP_216584.1 15609205 888742 complement(2325886..2326809) 1 NC_000962.3 Rv2068c, (MTCY49.07c), len: 307 aa. BlaC, class a beta-lactamase (see citation below), similar to many. Contains PS00013 Prokaryotic lipid attachment site near N-terminus, and PS00146 Beta-lactamase class-a active site.; Class a beta-lactamase BlaC 2326809 blaC 888742 blaC Mycobacterium tuberculosis H37Rv Class a beta-lactamase BlaC NP_216584.1 2325886 R 83332 CDS NP_216585.1 15609206 888723 2326944..2327501 1 NC_000962.3 Rv2069, (MTCY49.08), len: 185 aa. SigC, RNA polymerase sigma factor, ECF subfamily (see Gomez et al.,1997; Chen et al., 2000), similar to many.; RNA polymerase sigma factor, ECF subfamily, SigC 2327501 sigC 888723 sigC Mycobacterium tuberculosis H37Rv RNA polymerase sigma factor, ECF subfamily, SigC NP_216585.1 2326944 D 83332 CDS NP_216586.1 15609207 887566 complement(2327491..2328225) 1 NC_000962.3 Rv2070c, (MTCY49.09c), len: 244 aa. CobK,precorrin-6x reductase, similar to many.; Precorrin-6X reductase CobK 2328225 cobK 887566 cobK Mycobacterium tuberculosis H37Rv Precorrin-6X reductase CobK NP_216586.1 2327491 R 83332 CDS NP_216587.1 15609208 888521 complement(2328222..2328977) 1 NC_000962.3 Rv2071c, (MTCY49.10c), len: 251 aa. CobM,precorrin-3 methylase, similar to many. Contains PS00839 Uroporphyrin-III C-methyltransferase signature 1, and PS00840 Uroporphyrin-III C-methyltransferase signature 2.; Precorrin-3 methylase CobM (precorrin-4 C11-methyltransferase) 2328977 cobM 888521 cobM Mycobacterium tuberculosis H37Rv Precorrin-3 methylase CobM (precorrin-4 C11-methyltransferase) NP_216587.1 2328222 R 83332 CDS NP_216588.1 15609209 888321 complement(2328974..2330146) 1 NC_000962.3 Rv2072c, (MTCY49.11c), len: 390 aa. CobL,precorrin-6Y C(5,15)-methyltransferase (decarboxylating).; Precorrin-6Y C(5,15)-methyltransferase (decarboxylating) CobL 2330146 cobL 888321 cobL Mycobacterium tuberculosis H37Rv Precorrin-6Y C(5,15)-methyltransferase (decarboxylating) CobL NP_216588.1 2328974 R 83332 CDS NP_216589.1 15609210 887267 complement(2330214..2330963) 1 NC_000962.3 Rv2073c, (MTCY49.12c), len: 249 aa. Probable oxidoreductase, belonging to shortchain dehydrogenase reductase (SDR) family, similar to many.; Probable shortchain dehydrogenase 2330963 887267 Rv2073c Mycobacterium tuberculosis H37Rv Probable shortchain dehydrogenase NP_216589.1 2330214 R 83332 CDS NP_216590.1 15609211 888523 2330993..2331406 1 NC_000962.3 Rv2074, (MTCY49.13), len: 137 aa. Possible pyridoxine 5'-phosphate oxidase (PNPOx) (See Biswal et al.,2006). Similar to conserved hypothetical proteins.; Possible pyridoxamine 5'-phosphate oxidase (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) 2331406 888523 Rv2074 Mycobacterium tuberculosis H37Rv Possible pyridoxamine 5'-phosphate oxidase (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) NP_216590.1 2330993 D 83332 CDS NP_216591.1 15609212 888771 complement(2331416..2332879) 1 NC_000962.3 Rv2075c, (MTCY49.14c), len: 487 aa. Possibly exported or envelope protein; has potential signal peptide at N-terminus and hydrophobic stretch around residue 430. Predicted to be an outer membrane protein (See Song et al.,2008).; Possible hypothetical exported or envelope protein 2332879 888771 Rv2075c Mycobacterium tuberculosis H37Rv Possible hypothetical exported or envelope protein NP_216591.1 2331416 R 83332 CDS NP_216592.1 15609213 887296 complement(2333037..2333288) 1 NC_000962.3 Rv2076c, (MTCY49.15c), len: 83 aa. Conserved hypothetical protein, similar to many.; hypothetical protein 2333288 887296 Rv2076c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216592.1 2333037 R 83332 CDS NP_216593.1 15609214 887785 complement(2333323..2334294) 1 NC_000962.3 Rv2077c, (MTCY49.16c), len: 323 aa. Possible conserved transmembrane protein. Part of Mycobacterium tuberculosis protein family with Rv2542, Rv2079, Rv2797c,Rv0963c, Rv1949c. Hydrophobic stretches at C-terminus.; Possible conserved transmembrane protein 2334294 887785 Rv2077c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_216593.1 2333323 R 83332 CDS YP_177658.1 57116940 3205060 complement(2334295..2334594) 1 NC_000962.3 Rv2077A, len: 99 aa. Conserved hypothetical protein,similar to many.; hypothetical protein 2334594 3205060 Rv2077A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177658.1 2334295 R 83332 CDS NP_216594.1 15609215 888071 2335059..2335373 1 NC_000962.3 Rv2078, (MTCY49.17), len: 104 aa. Conserved hypothetical protein, similar to many.; hypothetical protein 2335373 888071 Rv2078 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216594.1 2335059 D 83332 CDS NP_216595.1 15609216 887333 2335355..2337325 1 NC_000962.3 Rv2079, (MTCY49.18), len: 656 aa. Conserved hypothetical protein; part of Mycobacterium tuberculosis protein family with Rv2542, Rv2077c, Rv2797c, Rv0963c,Rv1949c. Contains PS00120 Lipases, serine active site; hypothetical protein 2337325 887333 Rv2079 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216595.1 2335355 D 83332 CDS NP_216596.1 15609217 887351 2337306..2337869 1 NC_000962.3 Rv2080, (MTCY49.19), len: 187 aa. LppJ, lipoprotein; contains prokayotic lipoprotein modification site (PS00013) and signal sequence at N-terminus.; Lipoprotein LppJ 2337869 lppJ 887351 lppJ Mycobacterium tuberculosis H37Rv Lipoprotein LppJ NP_216596.1 2337306 D 83332 CDS NP_216597.1 15609218 887348 complement(2338065..2338505) 1 NC_000962.3 Rv2081c, (MTCY49.20c), len: 146 aa. Conserved transmembrane protein, similar to many. Hydrophobic stretch from aa 32-54.; Conserved transmembrane protein 2338505 887348 Rv2081c Mycobacterium tuberculosis H37Rv Conserved transmembrane protein NP_216597.1 2338065 R 83332 CDS NP_216598.1 15609219 887795 2338709..2340874 1 NC_000962.3 Rv2082, (MTCY49.21), len: 721 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis Rv0029, and to Rv3899c and Rv3900c which may be frameshifted.; hypothetical protein 2340874 887795 Rv2082 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216598.1 2338709 D 83332 CDS NP_216599.1 15609220 887281 2340871..2341815 1 NC_000962.3 Rv2083, (MTCY49.22), len: 314 aa. Conserved hypothetical protein. Similar to many e.g. Mycobacterium tuberculosis Rv3898c (110 aa) and Rv3897c (210 aa).; hypothetical protein 2341815 887281 Rv2083 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216599.1 2340871 D 83332 CDS NP_216600.1 15609221 887350 2341808..2342944 1 NC_000962.3 Rv2084, (MTCY49.23), len: 378 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2342944 887350 Rv2084 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216600.1 2341808 D 83332 CDS NP_216601.1 15609222 888138 2343027..2343332 1 NC_000962.3 Rv2085, (MTCY49.24), len: 101 aa. Conserved hypothetical protein, similar to but shorter than many transposases but we can find no sequence errors to account for the frameshifts. Contains possible helix-turn-helix motif at aa 33 to 54,(+3.11 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 2343332 888138 Rv2085 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216601.1 2343027 D 83332 CDS NP_216602.2 57116941 888128 2343311..2343916 1 NC_000962.3 Rv2086, (MTCY49.25), len: 201 aa. Conserved hypothetical protein, similarity to but shorter than many transposases but we can find no sequence errors to account for the frameshifts. Start changed since first submission (-16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 2343916 888128 Rv2086 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216602.2 2343311 D 83332 CDS NP_216603.2 57116942 887934 2343994..2344224 1 NC_000962.3 Rv2087, (MTCY49.27), len: 76 aa. Conserved hypothetical protein, similar to but shorter than transposases, but we can find no sequence errors to account for the frameshifts. Start changed since first submission (-45 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 2344224 887934 Rv2087 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216603.2 2343994 D 83332 CDS NP_216604.1 15609225 888322 2344411..2346180 1 NC_000962.3 Rv2088, (MTCY49.28), len: 589 aa. PknJ,transmembrane serine/threonine-protein kinase (see citation below). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: requires divalent cations for activity.; Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J) 2346180 pknJ 888322 pknJ Mycobacterium tuberculosis H37Rv Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J) NP_216604.1 2344411 D 83332 CDS NP_216605.1 15609226 887719 complement(2346197..2347324) 1 NC_000962.3 Rv2089c, (MTCY49.29c), len: 375 aa. PepE,dipeptidase, similar to many; contains PS00491 Aminopeptidase P and proline dipeptidase signature. Also similar to Mycobacterium tuberculosis peptidases Rv2861c,Rv0734, Rv2535c. Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al., 2010).; Dipeptidase PepE 2347324 pepE 887719 pepE Mycobacterium tuberculosis H37Rv Dipeptidase PepE NP_216605.1 2346197 R 83332 CDS NP_216606.1 15609227 887924 2347373..2348554 1 NC_000962.3 Rv2090, (MTCY49.30), len: 393 aa. Probable 5'-3' exonuclease, similar to exonuclease part of DNA polymerase. Belongs to family a of DNA polymerases.; Probable 5'-3' exonuclease 2348554 887924 Rv2090 Mycobacterium tuberculosis H37Rv Probable 5'-3' exonuclease NP_216606.1 2347373 D 83332 CDS NP_216607.1 15609228 887469 complement(2348558..2349292) 1 NC_000962.3 Rv2091c, (MTCY49.31c), len: 244 aa. Probable membrane protein; contains potential transmembrane region. Repetitive ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable membrane protein 2349292 887469 Rv2091c Mycobacterium tuberculosis H37Rv Probable membrane protein NP_216607.1 2348558 R 83332 CDS NP_216608.1 15609229 887736 complement(2349334..2352054) 1 NC_000962.3 Rv2092c, (MTCY49.32c), len: 906 aa. HelY,ATP-dependent DNA helicase, similar to many; contains PS00017 ATP/GTP-binding site motif A, PS00402 Binding-protein-dependent transport systems inner membrane component signature. Belongs to the SKI2 subfamily of helicases.; ATP-dependent DNA helicase HelY 2352054 helY 887736 helY Mycobacterium tuberculosis H37Rv ATP-dependent DNA helicase HelY NP_216608.1 2349334 R 83332 CDS NP_216609.1 15609230 888068 complement(2352103..2353029) 1 NC_000962.3 Rv2093c, (MT2154, MTCY49.33c), len: 308 aa. TatC,transmembrane protein, component of twin-arginine translocation protein export system (see citation below),similar to many. Belongs to the TatC family.; Sec-independent protein translocase transmembrane protein TatC 2353029 tatC 888068 tatC Mycobacterium tuberculosis H37Rv Sec-independent protein translocase transmembrane protein TatC NP_216609.1 2352103 R 83332 CDS NP_216610.1 15609231 888086 complement(2353046..2353297) 1 NC_000962.3 Rv2094c, (MT2155, MTCY49.34c), len: 83 aa. TatA,membrane-bound protein, component of twin-arginine translocation protein export system (see Berks et al.,2000), similar to many. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the TATA/E family.; Sec-independent protein translocase membrane-bound protein TatA 2353297 tatA 888086 tatA Mycobacterium tuberculosis H37Rv Sec-independent protein translocase membrane-bound protein TatA NP_216610.1 2353046 R 83332 CDS NP_216611.1 15609232 888403 complement(2353365..2354315) 1 NC_000962.3 Rv2095c, (MTCY49.35c), len: 316 aa. PafC, proteasome accessory factor C, similar to many. Contains possible helix-turn-helix motif at aa 25-46, (+2.92 SD). PafB|Rv2096c and PafC|Rv2095c interact (See Festa et al.,2007).; Proteasome accessory factor C PafC 2354315 pafC 888403 pafC Mycobacterium tuberculosis H37Rv Proteasome accessory factor C PafC NP_216611.1 2353365 R 83332 CDS NP_216612.1 15609233 888436 complement(2354312..2355310) 1 NC_000962.3 Rv2096c, (MTCY49.36c), len: 332 aa. PafB, proteasome accessory factor B, similar to many. PafB|Rv2096c and PafC|Rv2095c interact (See Festa et al., 2007).; Proteasome accessory factor B PafB 2355310 pafB 888436 pafB Mycobacterium tuberculosis H37Rv Proteasome accessory factor B PafB NP_216612.1 2354312 R 83332 CDS NP_216613.1 15609234 888460 complement(2355319..2356677) 1 NC_000962.3 Rv2097c, (MTCY49.37c), len: 452 aa. PafA, proteasome accessory factor A, similar to many. Belongs to the carboxylate amine/ammonia ligase family.; Proteasome accessory factor a PafA 2356677 pafA 888460 pafA Mycobacterium tuberculosis H37Rv Proteasome accessory factor a PafA NP_216613.1 2355319 R 83332 CDS NP_216616.1 15609237 888454 2358389..2360041 1 NC_000962.3 Rv2100, (MTCY49.40), len: 550 aa. Conserved hypothetical protein. Member of Mycobacterium tuberculosis REP13E12 repeat family with Rv1148c, Rv1945, Rv3467,Rv0094c, Rv1128c, Rv1587c, Rv1702c, Rv3466, Rv1588c.; hypothetical protein 2360041 888454 Rv2100 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216616.1 2358389 D 83332 CDS NP_216617.2 448824774 888635 2360240..2363281 1 NC_000962.3 Rv2101, (MTV020.01), len: 1013 aa. Probable helZ,helicase, similar to many. Nucleotide position 2361623 in the genome sequence has been corrected, A:C resulting in M462L.; Probable helicase HelZ 2363281 helZ 888635 helZ Mycobacterium tuberculosis H37Rv Probable helicase HelZ NP_216617.2 2360240 D 83332 CDS NP_216618.1 15609239 888639 2363391..2364107 1 NC_000962.3 Rv2102, (MTV020.02), len: 238 aa. Conserved hypothetical protein, similar to many. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; hypothetical protein 2364107 888639 Rv2102 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216618.1 2363391 D 83332 CDS NP_216619.1 15609240 888003 complement(2364086..2364520) 1 NC_000962.3 Rv2103c, (MTV020.03), len: 144 aa. Possible vapC37,toxin, part of toxin-antitoxin (TA) operon with Rv2104c,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis including Rv0749,Rv0277c, Rv2530c, Rv3320c, Rv2494, Rv2872, Rv0617, Rv1242 etc.; Possible toxin VapC37 Contains PIN domain 2364520 vapC37 888003 vapC37 Mycobacterium tuberculosis H37Rv Possible toxin VapC37 Contains PIN domain NP_216619.1 2364086 R 83332 CDS NP_216620.1 15609241 888014 complement(2364527..2364781) 1 NC_000962.3 Rv2104c, (MTV020.04), len: 84 aa. Possible vapB37,antitoxin, part of toxin-antitoxin (TA) operon with Rv2103c, see Arcus et al. 2005. Similar to others in M. tuberculosis including Rv2871, Rv1241, Rv2132, Rv3321c,Rv1113, Rv0657, Rv1560, etc.; Possible antitoxin VapB37 2364781 vapB37 888014 vapB37 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB37 NP_216620.1 2364527 R 83332 CDS NP_216621.1 15609242 888395 2365465..2365791 1 NC_000962.3 Rv2105, (MTCY261.01), len: 108 aa. Putative transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43,(100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2105 and Rv2106, the sequence UUUUAAAG (directly upstream of Rv2106) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Putative transposase for insertion sequence element IS6110 (fragment) 2365791 888395 Rv2105 Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 (fragment) NP_216621.1 2365465 D 83332 CDS NP_216622.2 448824775 888398 <2365740..2366726 1 NC_000962.3 Rv2106, (MTCY261.02), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2105 and Rv2106, the sequence UUUUAAAG (directly upstream of Rv2106) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa).; Probable transposase 2366726 888398 Rv2106 Mycobacterium tuberculosis H37Rv Probable transposase NP_216622.2 <2365740 D 83332 CDS YP_177858.1 57116945 887811 2367359..2367655 1 NC_000962.3 Rv2107, (MTCY261.03), len: 98 aa. PE22, Member of mycobacterial PE family (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; PE family protein PE22 2367655 PE22 887811 PE22 Mycobacterium tuberculosis H37Rv PE family protein PE22 YP_177858.1 2367359 D 83332 CDS YP_177859.1 57116946 887814 2367711..2368442 1 NC_000962.3 Rv2108, (MTCY261.04), len: 243 aa. PPE36, Member of the Mycobacterium tuberculosis PE family: N-terminus is similar to N-terminal region of Mycobacterium tuberculosis PPE family proteins. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; PPE family protein PPE36 2368442 PPE36 887814 PPE36 Mycobacterium tuberculosis H37Rv PPE family protein PPE36 YP_177859.1 2367711 D 83332 CDS NP_216625.1 15609246 887538 complement(2368983..2369729) 1 NC_000962.3 Rv2109c, (MTCY261.05c), len: 248 aa. PrcA,proteasome alpha-type subunit 1. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). prcBA genes encode a proteasome with broad substrate specificity (See Lin et al., 2006).; Proteasome alpha subunit PrcA; assembles with beta subunit PrcB 2369729 prcA 887538 prcA Mycobacterium tuberculosis H37Rv Proteasome alpha subunit PrcA; assembles with beta subunit PrcB NP_216625.1 2368983 R 83332 CDS NP_216626.1 15609247 887508 complement(2369726..2370601) 1 NC_000962.3 Rv2110c, (MTCY261.06c), len: 291 aa. PrcB,proteasome beta-type subunit 2. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). prcBA genes encode a proteasome with broad substrate specificity (See Lin et al., 2006).; Proteasome beta subunit PrcB; assembles with alpha subunit PrcA 2370601 prcB 887508 prcB Mycobacterium tuberculosis H37Rv Proteasome beta subunit PrcB; assembles with alpha subunit PrcA NP_216626.1 2369726 R 83332 CDS NP_216627.1 15609248 888788 complement(2370598..2370792) 1 NC_000962.3 Rv2111c, MTCY261.07c, len: 64 aa. Pup, prokaryotic ubiquitin-like protein (See Pearce et al., 2008). Highly similar to many. Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008).; Prokaryotic ubiquitin-like protein Pup 2370792 pup 888788 pup Mycobacterium tuberculosis H37Rv Prokaryotic ubiquitin-like protein Pup NP_216627.1 2370598 R 83332 CDS NP_216628.1 15609249 888290 complement(2370905..2372569) 1 NC_000962.3 Rv2112c, (MTCY261.08c), len: 554 aa. Dop, deamidase of Pup (See Streibel et al., 2009). Highly similar to many. Cofactor: ATP. Predicted to be an outer membrane protein (See Song et al., 2008).; Deamidase of pup Dop 2372569 dop 888290 dop Mycobacterium tuberculosis H37Rv Deamidase of pup Dop NP_216628.1 2370905 R 83332 CDS NP_216629.1 15609250 887731 2372630..2373823 1 NC_000962.3 Rv2113, (MTCY261.09), len: 397 aa. Probable integral membrane protein.; Probable integral membrane protein 2373823 887731 Rv2113 Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_216629.1 2372630 D 83332 CDS NP_216630.1 15609251 887803 2373834..2374457 1 NC_000962.3 Rv2114, (MTCY261.10), len: 207 aa. Conserved protein, similar to many.; hypothetical protein 2374457 887803 Rv2114 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216630.1 2373834 D 83332 CDS NP_216631.1 15609252 887297 complement(2374461..2376290) 1 NC_000962.3 Rv2115c, (MTCY261.11c), len: 609 aa. Mpa,mycobacterial proteasome ATPase, similar to many. Contains PS00674 AAA-protein family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Identified as a substrate for proteasomal degradation (See Pearce et al.,2006). Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008).; Mycobacterial proteasome ATPase Mpa 2376290 mpa 887297 mpa Mycobacterium tuberculosis H37Rv Mycobacterial proteasome ATPase Mpa NP_216631.1 2374461 R 83332 CDS NP_216632.1 15609253 886029 2376571..2377140 1 NC_000962.3 Rv2116, (MTCY261.12), len: 189 aa. LppK, conserved lipoprotein, similar to many. Contains N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Some similarity to Rv2376c. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Conserved lipoprotein LppK 2377140 lppK 886029 lppK Mycobacterium tuberculosis H37Rv Conserved lipoprotein LppK NP_216632.1 2376571 D 83332 CDS NP_216633.1 15609254 887685 2377148..2377441 1 NC_000962.3 Rv2117, (MTCY261.13), len: 97 aa. Conserved hypothetical protein, similar to many.; hypothetical protein 2377441 887685 Rv2117 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216633.1 2377148 D 83332 CDS NP_216634.1 15609255 887374 complement(2377470..2378312) 1 NC_000962.3 Rv2118c, (MTCY261.14c), len: 280 aa. S-adenosyl-l-methionine-dependent RNA methyltransferase (see citation below), similar to many. The larger catalytic C-terminal domain binds the cofactor S-adenosyl-l-methionine (AdoMet) and is involved in the transfer of methyl group from AdoMet to the substrate.; RNA methyltransferase 2378312 887374 Rv2118c Mycobacterium tuberculosis H37Rv RNA methyltransferase NP_216634.1 2377470 R 83332 CDS NP_216635.1 15609256 888413 2378386..2379222 1 NC_000962.3 Rv2119, (MTCY261.15), len: 278 aa. Conserved hypothetical protein, similar to many.; hypothetical protein 2379222 888413 Rv2119 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216635.1 2378386 D 83332 CDS NP_216636.1 15609257 887821 complement(2379245..2379727) 1 NC_000962.3 Rv2120c, (MTCY261.16c), len: 160 aa. Probable conserved integral membrane protein, similar to many.; Probable conserved integral membrane protein 2379727 887821 Rv2120c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216636.1 2379245 R 83332 CDS NP_216637.1 15609258 888689 complement(2379806..2380660) 1 NC_000962.3 Rv2121c, (MTCY261.17c), len: 284 aa. HisG, ATP phosphoribosyltransferase (see citation below), similar to many.; ATP phosphoribosyltransferase HisG 2380660 hisG 888689 hisG Mycobacterium tuberculosis H37Rv ATP phosphoribosyltransferase HisG NP_216637.1 2379806 R 83332 CDS YP_177860.1 57116947 888671 complement(2380663..2380944) 1 NC_000962.3 Rv2122c, (MTCY261.18), len: 93 aa. HisE (alternate gene name: irg1), phosphoribosyl-AMP cyclohydrolase (see citation below), similar to many. Note that previously misnamed hisI.; Phosphoribosyl-AMP pyrophosphatase HisE 2380944 hisE 888671 hisE Mycobacterium tuberculosis H37Rv Phosphoribosyl-AMP pyrophosphatase HisE YP_177860.1 2380663 R 83332 CDS YP_177861.1 57116948 888710 2381071..2382492 1 NC_000962.3 Rv2123, (MTCY261.19), len: 473 aa. PPE37 (alternate gene name: irg2), member of the Mycobacterium tuberculosis PPE family of proteins but the C-terminus is not repetitive (see citation below).; PPE family protein PPE37 2382492 PPE37 888710 PPE37 Mycobacterium tuberculosis H37Rv PPE family protein PPE37 YP_177861.1 2381071 D 83332 CDS NP_216640.1 15609261 888711 complement(2382489..2386067) 1 NC_000962.3 Rv2124c, (MTCY261.20c), len: 1192 aa. MetH,methionine synthase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to the vitamin-B12 dependent methionine synthase family.; 5-methyltetrahydrofolate--homocystein methyltransferase MetH (methionine synthase, vitamin-B12 dependent isozyme) (ms) 2386067 metH 888711 metH Mycobacterium tuberculosis H37Rv 5-methyltetrahydrofolate--homocystein methyltransferase MetH (methionine synthase, vitamin-B12 dependent isozyme) (ms) NP_216640.1 2382489 R 83332 CDS NP_216641.1 15609262 887798 2386293..2387171 1 NC_000962.3 Rv2125, (MTCY261.21), len: 292 aa. Conserved hypothetical protein, similar to many.; hypothetical protein 2387171 887798 Rv2125 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216641.1 2386293 D 83332 CDS YP_177862.1 57116949 887791 complement(2387202..2387972) 1 NC_000962.3 Rv2126c, (MTCY261.22c), len: 256 aa. PE_PGRS37,Possible PE_PGRS pseudogene fragment, similar to the Gly-rich C-terminus of many members of the Mycobacterium tuberculosis PGRS family.; PE-PGRS family protein PE_PGRS37 2387972 PE_PGRS37 887791 PE_PGRS37 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS37 YP_177862.1 2387202 R 83332 CDS YP_177863.1 57116950 887715 2388616..2390085 1 NC_000962.3 Rv2127, (MTCY261.26), len: 489 aa. AnsP1,L-asparagine permease, integral membrane protein similar to many. Contains PS00218 Amino acid permeases signature. Seems to belong to the APC family.; L-asparagine permease AnsP1 2390085 ansP1 887715 ansP1 Mycobacterium tuberculosis H37Rv L-asparagine permease AnsP1 YP_177863.1 2388616 D 83332 CDS NP_216644.1 15609265 887764 2390085..2390288 1 NC_000962.3 Rv2128, (MTCY26.27), len: 67 aa. Conserved transmembrane protein, similar to many.; Conserved transmembrane protein 2390288 887764 Rv2128 Mycobacterium tuberculosis H37Rv Conserved transmembrane protein NP_216644.1 2390085 D 83332 CDS NP_216645.1 15609266 887841 complement(2390308..2391189) 1 NC_000962.3 Rv2129c, (MTCY261.28), len: 293 aa. Probable oxidoreductase, similar to many e.g. FABG_SYNY3|P73826 3-oxoacyl-[acyl-carrier protein] reductase (240 aa), FASTA scores: opt: 241, E(): 5.1e-17, (32.7% identity in 196 aa overlap); etc. Also similar to a number of other Mycobacterium tuberculosis oxidoreductases e.g. MTCY210.04 (34.1% identity in 217 aa overlap).; Probable oxidoreductase 2391189 887841 Rv2129c Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_216645.1 2390308 R 83332 CDS NP_216646.1 15609267 887492 complement(2391215..2392459) 1 NC_000962.3 Rv2130c, (MTCY261.29c), len: 414 aa. MshC,cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase (see Rawat et al., 2002), similar to several cysteinyl-tRNA synthetases e.g. SYC_ECOLI|P21888 cysteinyl-tRNA synthetase from Escherichia coli (461 aa),FASTA scores: opt: 535, E(): 0, (37.0% identity in 370 aa overlap); etc. Also similar to Mycobacterium tuberculosis cysS|Rv3580c|MTCY06G11.27c, (35.8% identity in 372 aa overlap). Contains a match to Pfam entry PF01406 tRNA synthetases class I (C). Previously known as cysS2.; Cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase MshC 2392459 mshC 887492 mshC Mycobacterium tuberculosis H37Rv Cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase MshC NP_216646.1 2391215 R 83332 CDS NP_216647.1 15609268 888142 complement(2392517..2393320) 1 NC_000962.3 Rv2131c, (MTCY270.37), len: 267 aa. CysQ,monophosphatase, equivalent to CYSQ_MYCLE|P46726 cysQ protein homolog from Mycobacterium leprae (289 aa), FASTA scores: opt: 1374, E(): 0, (77.3% identity in 264 aa overlap). Contains inositol monophosphatase family signature 1 (PS00629), significance uncertain. Seems to belong to the inositol monophosphatase family. Cofactor: Mg2+. Inhibited by Li+; PAPase activity is inhibited by Na+ and K+, but IMPase activity is not (See Gu et al., 2006; Hatzios et al., 2008).; Monophosphatase CysQ 2393320 cysQ 888142 cysQ Mycobacterium tuberculosis H37Rv Monophosphatase CysQ NP_216647.1 2392517 R 83332 CDS NP_216648.1 15609269 887827 2393411..2393641 1 NC_000962.3 Rv2132, (MTCY270.36c), len: 76 aa. Conserved hypothetical protein. Function unknown but belongs to Mycobacterium tuberculosis protein family including Rv2871,Rv1241, Rv3321c, Rv1113, Rv0657c, Rv1560, Rv2104c, etc. Similarity to Mycobacterium tuberculosis protein Rv2871 (AL021924|MTV020_4, 84 aa). FASTA score: opt: 142, E(): 0.00036; 41.8% identity in 55 aa overlap; hypothetical protein 2393641 887827 Rv2132 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216648.1 2393411 D 83332 CDS NP_216649.1 15609270 887616 complement(2393851..2394639) 1 NC_000962.3 Rv2133c, (MTCY270.35), len: 262 aa. Conserved hypothetical protein. Function: unknown but equivalent to hypothetical Mycobacterium leprae protein, Q49774. FASTA best: Q49774 B2126_C1_150 (262 aa) opt: 1447, E(): 0; (79.0% identity in 262 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2394639 887616 Rv2133c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216649.1 2393851 R 83332 CDS NP_216650.1 15609271 887844 complement(2394650..2395237) 1 NC_000962.3 Rv2134c, (MTCY270.34), len: 195 aa. Conserved protein. Function: unknown but equivalent to hypothetical Mycobacterium leprae protein, Q49789. FASTA best: Q49789 B2126_C3_228, opt: 1192, E(): 0 (91.1% identity in 192 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2395237 887844 Rv2134c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216650.1 2394650 R 83332 CDS NP_216651.1 15609272 887273 complement(2395301..2396011) 1 NC_000962.3 Rv2135c, (MTCY270.33), len: 236 aa. Conserved protein. Function: unknown but equivalent to hypothetical Mycobacterium leprae protein, Q49773. FASTA best: Q49773 B2126_C1_148 opt: 1183, E() : 0; (74.8% identity in 250 aa overlap), also similar in C-terminus to PMG2_ECOLI P36942 probable phosphoglycerate mutase 2 (215 aa), FASTA scores; opt: 212, E(): 2.5e-07 27.9% identity in 190 aa overlap; and to Rv2228 and Rv2419c; hypothetical protein 2396011 887273 Rv2135c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216651.1 2395301 R 83332 CDS NP_216652.1 15609273 887612 complement(2396008..2396838) 1 NC_000962.3 Rv2136c, (MTCY270.32), len: 276 aa. Possible conserved transmembrane protein, very similar to hypothetical Mycobacterium leprae protein Q49783. FASTA best: Q49783 B2126_C2_190 opt: 1023, E(): 0; (82.4% identity in 187 aa over lap) similar to BACA_ECOLI P31054 bacitracin resistance protein (273 aa) opt: 477, E(): 7e-26, (35.6% identity in 267 aa overlap); Possible conserved transmembrane protein 2396838 887612 Rv2136c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_216652.1 2396008 R 83332 CDS NP_216653.1 15609274 888009 complement(2396902..2397315) 1 NC_000962.3 Rv2137c, (MTCY270.31), len: 137 aa. Conserved hypothetical protein. C-terminus is very similar to hypothetical Mycobacterium leprae protein B2126_C2_188 (150 aa). FASTA best: Q49782 B2126_C2_188. (150 aa) opt: 469,E(): 9.6e-28; (77.2% identity in 101 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2397315 888009 Rv2137c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216653.1 2396902 R 83332 CDS NP_216654.1 15609275 887303 2397330..2398406 1 NC_000962.3 Rv2138, (MTCY270.30c), len: 358 aa. Probable lppL,conserved lipoprotein, with appropriately placed lipoprotein signature (PS00013) strongly similar to hypothetical Mycobacterium leprae protein, Q49806. FASTA best: Q49806 B2126_F3_142. (298 aa) opt: 1495, E(): 0; (75.3% identity in 300 aa overlap).; Probable conserved lipoprotein LppL 2398406 lppL 887303 lppL Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppL NP_216654.1 2397330 D 83332 CDS NP_216655.1 15609276 887326 2398720..2399793 1 NC_000962.3 Rv2139, (MTCY270.29c), len: 357 aa. Probable pyrD,dihydroorotate dehydrogenase ; contains dihydroorotate dehydrogenase signatures 1 and 2 (PS00911, PS00912). FASTA best: PYRD_MYCLE P46727 dihydroorotate dehydrogenase (309 aa) opt: 1653, E(): 0; (82.6% identity in 304 aa overlap); Probable dihydroorotate dehydrogenase PyrD 2399793 pyrD 887326 pyrD Mycobacterium tuberculosis H37Rv Probable dihydroorotate dehydrogenase PyrD NP_216655.1 2398720 D 83332 CDS NP_216656.1 15609277 888118 complement(2399798..2400328) 1 NC_000962.3 Rv2140c, (MTCY270.28), len: 176 aa. TB18.6,conserved protein; shows good similarity to hypothetical proteins from Streptomyces coelicolor (177 aa; 58% identity) >emb|CAC32358.1| (AL583945) and to 17.1 kDa Escherichia coli protein YbhB. FASTA best: YBHB_ECOLI P12994 hypothetical 17.1 kDa protein (158 aa) opt: 465 E( ): 2e-23; (46.2% identity in 156 aa overlap).; Conserved protein TB18.6 2400328 TB18.6 888118 TB18.6 Mycobacterium tuberculosis H37Rv Conserved protein TB18.6 NP_216656.1 2399798 R 83332 CDS YP_177864.1 57116951 887384 complement(2400376..2401722) 1 NC_000962.3 Rv2141c, (MTCY270.27), len: 448 aa. Conserved protein. Shows some similarity to conserved hypothetical proteins and to acetylornithine deacetylase and succinyl-diaminopimelate desuccinylase and contains ArgE/dapE/ACY1/CPG2/yscS family signature 1 (PS00758). FASTA best: CBPS_YEAST P27614 carboxypeptidases precursor (576 aa) opt: 234, E(): 4.3e-08; (24.3% identity in 412 aa overlap). Previously named dapE2. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; hypothetical protein 2401722 887384 Rv2141c Mycobacterium tuberculosis H37Rv hypothetical protein YP_177864.1 2400376 R 83332 CDS NP_216658.1 15609279 888770 complement(2402193..2402510) 1 NC_000962.3 Rv2142c, (MTCY270.26), len: 105 aa. Possible parE2,toxin, part of toxin-antitoxin (TA) operon with Rv2142A (See Pandey and Gerdes, 2005).; Possible toxin ParE2 2402510 parE2 888770 parE2 Mycobacterium tuberculosis H37Rv Possible toxin ParE2 NP_216658.1 2402193 R 83332 CDS YP_007410825.1 448816952 14515895 complement(2402507..2402722) 1 NC_000962.3 Rv2142A, len: 71 aa. Possible parD2, antitoxin, part of toxin-antitoxin (TA) operon with Rv2142c (See Pandey and Gerdes, 2005).; Possible antitoxin ParD2 2402722 parD2 14515895 parD2 Mycobacterium tuberculosis H37Rv Possible antitoxin ParD2 YP_007410825.1 2402507 R 83332 CDS NP_216659.1 15609280 887406 2402977..2404035 1 NC_000962.3 Rv2143, (MTCY270.25c), len: 352 aa. Conserved hypothetical protein, strongly similar to two hypothetical mycobacterial proteins Rv2030c 2.1e-50 and Rv0571c from position 120 (Q50819; Q50111). FASTA best: Q50819 opt: 882,E() 0; (61.1% identity in 226 aa overlap). Also similar to AL021942|MTV039_9 (443 aa), FASTA scores: opt: 592, E(): 5e-30; 46.9% identity in 224 aa overlap; hypothetical protein 2404035 887406 Rv2143 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216659.1 2402977 D 83332 CDS NP_216660.1 15609281 888202 complement(2404165..2404521) 1 NC_000962.3 Rv2144c, (MTCY270.24), len: 118 aa. Probable transmembrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable transmembrane protein 2404521 888202 Rv2144c Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_216660.1 2404165 R 83332 CDS NP_216661.1 15609282 888224 complement(2404616..2405398) 1 NC_000962.3 Rv2145c, (MTCY270.23), len: 260 aa. Wag31 (alternate gene name: ag84). Function unknown but corresponds to antigen 84 of Mycobacterium tuberculosis (wag31) (see Hermans et al., 1995). Predicted to contain significant amount of coiled coil structure. Some similarity to Rv1682 and Rv2927c. FASTA best: AG84_MYCTU P46816 antigen 84. Wag31|Rv2145c and PbpB|Rv2163c have been shown to interact; cleavage of PbpB|Rv2163c by Rv2869c under conditions of oxidative stress is prevented by Wag31|Rv2145c (See Mukherjee et al., 2009).; Diviva family protein Wag31 2405398 wag31 888224 wag31 Mycobacterium tuberculosis H37Rv Diviva family protein Wag31 NP_216661.1 2404616 R 83332 CDS NP_216662.1 15609283 888759 complement(2405666..2405956) 1 NC_000962.3 Rv2146c, (MTCY270.22), len: 96 aa. Possible conserved transmembrane protein, orthologs present in M. leprae, ML0921 (96 aa) and Streptomyces coelicolor. Second start taken GTG alternative upstream but much less probable in TBParse. FASTA best: Q44935 similar to a hypothetical integral membrane prot EIN (97 aa) opt: 105, E(): 0.093; (25.3% identity in 87 aa overlap). >emb|CAC31302.1| (AL583920) possible membrane protein ML0921 [Mycobacterium leprae] E(): 5e-32 (76% identity in 96 aa overlap); Possible conserved transmembrane protein 2405956 888759 Rv2146c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_216662.1 2405666 R 83332 CDS NP_216663.1 15609284 887394 complement(2406118..2406843) 1 NC_000962.3 Rv2147c, (MTCY270.21), len: 241 aa. Conserved hypothetical protein, similar to conserved hypothetical proteins in Mycobacterium leprae ML0920 (210 aa) and Streptomyces coelicolor. FASTA scores: >emb|CAC31301.1| (AL583920) hypothetical protein ML0920 hypothetical protein (210 aa) opt: 1242, E(): 5.7e-74; 83.486% identity in 218 aa overlap. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2406843 887394 Rv2147c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216663.1 2406118 R 83332 CDS NP_216664.1 15609285 888127 complement(2406840..2407616) 1 NC_000962.3 Rv2148c, (MTCY270.20), len: 258 aa. Conserved protein; should belong to the YGGS/YBL036C/F09E5.8 family. FASTA best: AB003132|AB003132_5 Corynebacterium glutamicum gene (221 aa) opt: 440, E(): 2.3e-23; 42.8% identity in 236 aa overlap; and YPI1_VIBAL P52055 hypothetical protein in pilt-proc intergenic region in Vibrio alginolyticus. opt: 266, E(): 1.8e-11; 27.9% identity in 244 aa overlap.; hypothetical protein 2407616 888127 Rv2148c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216664.1 2406840 R 83332 CDS NP_216665.1 15609286 888121 complement(2407622..2408374) 1 NC_000962.3 Rv2149c, (MTCY270.19), len: 250 aa. YfiH; corresponds to 25.3 kDa YfiH protein in ftsZ 3' region of Streptomyces griseus, and to YfiH proteins in other bacteria. Belongs to UPF0124 Family. FASTA best: YFIH_STRGR P45496, (246 aa) opt: 722, E(): 1.9e-37; (49.4% identity in 245 aa overlap); Conserved protein YfiH 2408374 yfiH 888121 yfiH Mycobacterium tuberculosis H37Rv Conserved protein YfiH NP_216665.1 2407622 R 83332 CDS NP_216666.1 15609287 888369 complement(2408385..2409524) 1 NC_000962.3 Rv2150c, (MTCY270.18), len: 379 aa. FtsZ, cell division protein (see Dziadek et al., 2002). Contains FtsZ protein signature 2 (PS01135). FASTA best: FTSZ_STRCO P45500 cell division protein FtsZ (399 aa) opt: 1674, E(): 0; (77.3% identity in 339 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006).; Cell division protein FtsZ 2409524 ftsZ 888369 ftsZ Mycobacterium tuberculosis H37Rv Cell division protein FtsZ NP_216666.1 2408385 R 83332 CDS NP_216667.1 15609288 888367 complement(2409697..2410641) 1 NC_000962.3 Rv2151c, (MTCY270.17), len: 314 aa. Possible ftsQ,cell division protein, with some homology to FTSQ_STRGR|P45503 cell division protein ftsq homolog from Streptomyces griseus (208 aa), FASTA scores: opt: 204, E(): 4e-05; (30.6% identity in 193 aa overlap).; Possible cell division protein FtsQ 2410641 ftsQ 888367 ftsQ Mycobacterium tuberculosis H37Rv Possible cell division protein FtsQ NP_216667.1 2409697 R 83332 CDS NP_216668.1 15609289 887983 complement(2410638..2412122) 1 NC_000962.3 Rv2152c, (MTCY270.16), len: 494 aa. Probable murC,UDP-N-acetylmuramate-alanine ligase (see citation below),similar to others e.g. MURC_ECOLI|P17952 (491 aa), FASTA scores: opt: 764, E(): 0, (36.9% identity in 474 aa overlap); etc.; Probable UDP-N-acetylmuramate-alanine ligase MurC 2412122 murC 887983 murC Mycobacterium tuberculosis H37Rv Probable UDP-N-acetylmuramate-alanine ligase MurC NP_216668.1 2410638 R 83332 CDS NP_216669.1 15609290 888036 complement(2412119..2413351) 1 NC_000962.3 Rv2153c, (MTCY270.15), len: 410 aa. Probable murG, UPD-N-acetylglucosamine-N-acetylmuramyl- (pentapeptide)pyrophosphoryl-undecaprenol-N- acetylglucosamine transferase (see citation below), similar to others e.g. MURG_BACSU[P37585 murg protein from Bacilus subtilis (363 aa), FASTA score: opt: 494, E(): 1.1e-20, (27.9% identity in 365 aa overlap); etc.; Probable UPD-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase MurG 2413351 murG 888036 murG Mycobacterium tuberculosis H37Rv Probable UPD-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase MurG NP_216669.1 2412119 R 83332 CDS NP_216670.1 15609291 887916 complement(2413348..2414922) 1 NC_000962.3 Rv2154c, (MTCY270.14), len: 524 aa. Probable ftsW,cell division protein, related to MTCY10H4.17c, 3.2e-17. FASTA best: SP5E_BACSU P07373 stage V sporulation protein E (366 aa) opt: 755, E(): 1.6e-33; (38.4% identity in 357 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006).; FtsW-like protein FtsW 2414922 ftsW 887916 ftsW Mycobacterium tuberculosis H37Rv FtsW-like protein FtsW NP_216670.1 2413348 R 83332 CDS NP_216671.3 448824776 888000 complement(2414934..2416394) 1 NC_000962.3 Rv2155c, (MTCY270.13), len: 486 aa. Probable murD,UDP-N-acetylmuramoylalanine-D-glutamate ligase (see citation below), similar to others e.g. MURD_BACSU|Q03522 (451 aa), FASTA scores: opt: 534, E(): 2.7e-25, (28.8% identity in 483 aa overlap); etc. Contains PS01011 Folylpolyglutamate synthase signature 1.; Probable UDP-N-acetylmuramoylalanine-D-glutamate ligase MurD 2416394 murD 888000 murD Mycobacterium tuberculosis H37Rv Probable UDP-N-acetylmuramoylalanine-D-glutamate ligase MurD NP_216671.3 2414934 R 83332 CDS NP_216672.1 15609293 888098 complement(2416396..2417475) 1 NC_000962.3 Rv2156c, (MTCY270.12), len: 359 aa. Probable murX,phospho-N-acetylmuramoyl-pentappeptidetransferase (see citation below), similar to others e.g.MRAY_ECOLI|P15876 (360 aa), FASTA scores: opt: 572, E(): 2.7e-29, (35.8% identity in 344 aa overlap); etc.; Probable phospho-N-acetylmuramoyl-pentappeptidetransferase MurX 2417475 murX 888098 murX Mycobacterium tuberculosis H37Rv Probable phospho-N-acetylmuramoyl-pentappeptidetransferase MurX NP_216672.1 2416396 R 83332 CDS NP_216673.1 15609294 887826 complement(2417472..2419004) 1 NC_000962.3 Rv2157c, (MTCY270.11), len: 510 aa. Probable murF,UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase (UDP-murnac-pentapeptide synthetase) (see citation below), also related to other Mycobacterium tuberculosis mur gene products. FASTA best: MURF_ECOLI|P11880 (452 aa),opt: 515, E(): 2.6e-24, (31.9% identity in 511 aa overlap).; Probable UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase MurF 2419004 murF 887826 murF Mycobacterium tuberculosis H37Rv Probable UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase MurF NP_216673.1 2417472 R 83332 CDS NP_216674.1 15609295 887252 complement(2419001..2420608) 1 NC_000962.3 Rv2158c, (MTCY270.10), len: 535 aa. Probable murE,UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase; UDP-N-acetylmuramyl-tripeptide synthetase (see citation below), also related to other Mycobacterium tuberculosis mur gene products. FASTA best: MURE_BACSU|Q03523 (494 aa), opt: 1020, E(): 0, (40.1% identity in 476 aa overlap).; Probable UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase MurE 2420608 murE 887252 murE Mycobacterium tuberculosis H37Rv Probable UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase MurE NP_216674.1 2419001 R 83332 CDS NP_216675.1 15609296 887236 complement(2420631..2421665) 1 NC_000962.3 Rv2159c, (MTCY270.09), len: 344 aa. Conserved protein; some similarity to hypothetical protein from Streptomyces coelicolor SC1A6.09c (337 aa, 29% identity). Smith-Waterman scores: >pir||T28690 hypothetical protein -Streptomyces coelicolor >gi|3127841|emb|CAA18907.1| (AL023496) Expect = 2e-18. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 2421665 887236 Rv2159c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216675.1 2420631 R 83332 CDS YP_177660.1 57116952 3205077 complement(2421643..2422278) 1 NC_000962.3 Rv2160A, len: 211 aa. Conserved hypothetical protein, possibly a TetR-family transcriptional regulator,similar to N-terminal half of AL512667_12|Q9AD73|SCK31.01c putative TetR-family transcriptional regulator from Streptomyces coelicolor (200 aa), FASTA scores: opt: 285,E(): 1.4e-08, (51.042% identity in 96 aa overlap). Next gene, Rv2160c, is similar to C-terminal half of 2SCK31.01c suggesting possible frameshift near 2421978 but sequence of this region has been checked and is also identical in strain CDC1551. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 2422278 3205077 Rv2160A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177660.1 2421643 R 83332 CDS NP_216676.1 15609297 886254 complement(2421662..2422003) 1 NC_000962.3 Rv2160c, (MTCY270.08), len: 113 aa. Conserved hypothetical protein, possibly a TetR-family transcriptional regulator, similar to C-terminal half of AL512667_12|Q9AD73|SCK31.01c putative TetR-family transcriptional regulator from Streptomyces coelicolor (200 aa), while Rv2160A is similar to the N-terminal half of 2SCK31.01c. This suggests possible frameshift near 2421978 but sequence of this region has been checked and is also identical in strain CDC1551. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 2422003 886254 Rv2160c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216676.1 2421662 R 83332 CDS NP_216677.1 15609298 887978 complement(2422271..2423137) 1 NC_000962.3 Rv2161c, (MTCY270.07), len: 288 aa. Conserved protein; shows some similarity to protein involved in lincomycin production and to other M. tuberculosis proteins e.g. Rv0953c, Rv0791c, Rv0132c, Rv2951c, Rv1855c. FASTA best: Q54379 (78-11) lincomycin production genes (295 aa) opt: 243, E(): 2.4e-09; (29.5% identity in 285 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 2423137 887978 Rv2161c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216677.1 2422271 R 83332 CDS YP_177865.1 57116953 887300 complement(2423240..2424838) 1 NC_000962.3 Rv2162c, (MTCY270.06), len: 532 aa. PE_PGRS38,Member of M. tuberculosis PE_PGRS family (see citations below). FASTA score: Y03A_MYCTU Q 10637 hypothetical glycine-rich 49.6 kDa protein (603 aa) op t: 1798 z-score: 1220.0 E(): 0; (55.4% identity in 590 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE-PGRS family protein PE_PGRS38 2424838 PE_PGRS38 887300 PE_PGRS38 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS38 YP_177865.1 2423240 R 83332 CDS NP_216679.1 15609300 887949 complement(2425048..2427087) 1 NC_000962.3 Rv2163c, (MTCY270.05), len: 679 aa. Probable pbpB,penicillin-binding membrane protein, similar to many bacterial PBP2 proteins e.g. P11882|PBP2_NEIME|PENA|NMA2072|NMB0413 penicillin-binding protein 2 (pbp-2) from Neisseria meningitidis (serogroups a and B) (581 aa), FASTA scores: opt: 665, E(): 1.6e-31,(33.2% identity in 591 aa overlap); etc. Also similar to Rv0016c and Rv2864c from Mycobacterium tuberculosis (2.8e-10). Contains PS00017 possible ATP/GTP-binding site motif A (P-loop) near C-terminus. FASTA best: PBP2_NEIME P11882 penicillin-binding protein 2 (pbp-2). (581 aa) opt: 665, E(): 1.6e-31; (33.2% identity in 591 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006). Cleavage of PbpB|Rv2163c by Rv2869c under conditions of oxidative stress is prevented by Wag31|Rv2145c (See Mukherjee et al., 2009).; Probable penicillin-binding membrane protein PbpB 2427087 pbpB 887949 pbpB Mycobacterium tuberculosis H37Rv Probable penicillin-binding membrane protein PbpB NP_216679.1 2425048 R 83332 CDS NP_216680.1 15609301 887217 complement(2427084..2428238) 1 NC_000962.3 Rv2164c, (MTCY270.04), len: 384 aa. Probable pro-rich conserved membrane protein, equivalent to ML0907|AL022602 putative conserved membrane protein from Mycobacterium leprae (377 aa) (AL022602), FASTA scores: opt: 1495, E(): 1.7e-56, (62.217% identity in 397 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved proline rich membrane protein 2428238 887217 Rv2164c Mycobacterium tuberculosis H37Rv Probable conserved proline rich membrane protein NP_216680.1 2427084 R 83332 CDS NP_216681.4 448824777 888462 complement(2428235..2429425) 1 NC_000962.3 Rv2165c, (MTCY270.03), len: 396 aa. Conserved protein; shows strong similarity to several hypothetical bacterial proteins but has extra 80 aa residues at N-terminus FASTA best: YLXA_BACSU Q07876 hypothetical 35.3 kDa protein in ftsl (311 aa) opt: 781, E(): 0; (45.6% identity in 296 aa overlap), belongs to the YABC (E.coli),YLXA (B.subtilis) family; hypothetical protein 2429425 888462 Rv2165c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216681.4 2428235 R 83332 CDS NP_216682.1 15609303 888261 complement(2429427..2429858) 1 NC_000962.3 Rv2166c, (MTCY270.02), len: 143 aa. Conserved protein; shows strong similarity to several hypothetical bacterial proteins such as YLLB_BACSU P55343. Is equivalent to Mycobacterium leprae hypothetical protein ML0905 (143 aa, 92% identity) MLCB268.11c >sp|O69561|YL66_MYCLE hypothetical 16.1 KDA protein ML0905 >gi|3080482|emb|CAA18677.1|(AL022602) >gi|13092975|emb|CAC31286.1|(AL583920). FASTA scores: ML0905|ML0905 conserved hypothetical protein (143 aa) opt: 873, E(): 3.1e-52; 92.254% identity in 142 aa overlap; YLLB_BACSU P55343 hypothetical 16.6 kDa protein (143 aa) opt: 340, E(): 3.6e-17; (35.0% identity in 143 aa overlap). Belongs to the YABB (E.coli), YLLB (B.subtilis), MG221 (M.genitalium) family; hypothetical protein 2429858 888261 Rv2166c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216682.1 2429427 R 83332 CDS NP_216683.2 448824778 888197 complement(2430159..>2431145) 1 NC_000962.3 Rv2167c, (MTCY270.01), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2167c and Rv2168c, the sequence UUUUAAAG (directly upstream of Rv2167c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (- 18 aa).; Probable transposase >2431145 888197 Rv2167c Mycobacterium tuberculosis H37Rv Probable transposase NP_216683.2 2430159 R 83332 CDS NP_216684.1 15609305 888459 complement(2431094..2431420) 1 NC_000962.3 Rv2168c, (MTV021.01c), len: 108 aa. Putative transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43,(100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2167c and Rv2168c, the sequence UUUUAAAG (directly upstream of Rv2167c) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Putative transposase for insertion sequence element IS6110 (fragment) 2431420 888459 Rv2168c Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 (fragment) NP_216684.1 2431094 R 83332 CDS NP_216685.1 15609306 888257 complement(2431565..2431969) 1 NC_000962.3 Rv2169c, (MTV021.02c), len: 134 aa. Probable conserved transmembrane protein, with orthologs in M. leprae, ML0904 probable membrane protein (134 aa), and Streptomyces coelicolor. FASTA scores with ML0904, opt: 767, E(): 5.1e-43; 86.567% identity in 134 aa overlap. emb|CAA18678.1| (AL022602) >gi|13092974|emb|CAC31285.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 2431969 888257 Rv2169c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216685.1 2431565 R 83332 CDS NP_216686.1 15609307 888170 2432235..2432855 1 NC_000962.3 Rv2170, (MTV021.03), len: 206 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to hypothetical protein ML0903 (210 aa) from Mycobacterium leprae. FASTA scores: ML0903 conserved hypothetical protein (210 aa) opt: 1045, E(): 9.1e-57; 77.143% identity in 210 aa overlap. >emb|CAA18679.1| (AL022602) >gi|13092973|emb|CAC31284.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; GCN5-related N-acetyltransferase 2432855 888170 Rv2170 Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_216686.1 2432235 D 83332 CDS NP_216687.1 15609308 887522 2432951..2433634 1 NC_000962.3 Rv2171, (MTV021.04), len: 227 aa. Probable lppM,conserved lipoprotein; contains putative signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Has hydrophobic stretch at C-terminus and also contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. Unknown but equivalent to Mycobacterium leprae lipoprotein ML0902 (239 aa). FASTA scores: opt: 1083, E(): 2.4e-56; 75.446% identity in 224 aa overlap (5-227:16-239) >emb|CAA18680.1| (AL022602) >gi|13092972|emb|CAC31283.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved lipoprotein LppM 2433634 lppM 887522 lppM Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppM NP_216687.1 2432951 D 83332 CDS NP_216688.1 15609309 888147 complement(2433631..2434536) 1 NC_000962.3 Rv2172c, (MTV021.05c), len: 301 aa. Conserved protein, equivalent to Mycobacterium leprae conserved hypothetical protein ML0901 (304 aa). FASTA scores: opt: 1656, E(): 7.7e-98; 81.271% identity in 299 aa overlap (1-299:1-299) >emb|CAA18681.1| (AL022602) >gi|13092971|emb|CAC31282.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2434536 888147 Rv2172c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216688.1 2433631 R 83332 CDS NP_216689.1 15609310 888334 2434847..2435905 1 NC_000962.3 Rv2173, (MTV021.06), len: 352 aa. Probable idsA2,geranylgeranyl pyrophosphate synthase, similar to many e.g. Q54193 geranylgeranyl pyrophosphate synthase from Streptomyces griseus (425 aa). Contains PS00723 and PS00444Polyprenyl synthetases signature 1 and 2. FASTA scores: sptr|Q54193|Q54193 geranylgeranyl pyrophosphate synthase (425 aa) opt: 744, E(): 0; 39.2% identity in 352 aa overlap.; Probable geranylgeranyl pyrophosphate synthetase IdsA2 (ggppsase) (GGPP synthetase) (geranylgeranyl diphosphate synthase) 2435905 idsA2 888334 idsA2 Mycobacterium tuberculosis H37Rv Probable geranylgeranyl pyrophosphate synthetase IdsA2 (ggppsase) (GGPP synthetase) (geranylgeranyl diphosphate synthase) NP_216689.1 2434847 D 83332 CDS NP_216690.1 15609311 887528 2435909..2437459 1 NC_000962.3 Rv2174, (MTV021.07), len: 516 aa. MptA (mannopyranosyltransferase A) (See Mishra et al., 2007). Possible conserved integral membrane protein, similar to some hypothetical mycobacterial proteins e.g. Mycobacterium leprae ML0899 probable integral-membrane protein (505 aa) and MLCL536_26 (593 aa). FASTA scores: ML0899 opt: 2715; 78.884% identity in 502 aa overlap and gp|Z99125|MLCL536_26 Mycobacterium leprae cosmid L536. (593 aa) opt: 552, E(): 7.1e-30; 31.6% identity in 513 aa overlap. Also similar to Rv1459c. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003).; Alpha(1->6)mannosyltransferase Possible conserved integral membrane protein 2437459 mptA 887528 mptA Mycobacterium tuberculosis H37Rv Alpha(1->6)mannosyltransferase Possible conserved integral membrane protein NP_216690.1 2435909 D 83332 CDS NP_216691.1 15609312 887852 complement(2437446..2437886) 1 NC_000962.3 Rv2175c, (MTV021.08c), len: 146 aa. Conserved protein, possibly involved in regulation. Contains possible helix-turn-helix domain at aa 31-52 (Score 1042, +2.74 SD). Equivalent to Mycobacterium leprae ML0898 putative DNA-binding protein (134 aa). FASTA scores: opt: 747; 82.090% identity in 134 aa overlap (AL022602) >gi|13092969|emb|CAC31279.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Conserved regulatory protein 2437886 887852 Rv2175c Mycobacterium tuberculosis H37Rv Conserved regulatory protein NP_216691.1 2437446 R 83332 CDS NP_216692.1 15609313 888340 2437941..2439140 1 NC_000962.3 Rv2176, (MTV021.09), len: 399 aa. Probable pknL,transmembrane serine/threonine-protein kinase (see citation below), similar to many e.g. MLCB1770_9 (622 aa). Lacks C-terminal domain and ends with putative transmembrane segment. Contains PS00108 Serine/Threonine protein kinases active-site signature. FASTA scores: Z70722|MLC B1770_9 Mycobacterium leprae cosmid B1770 (622 aa) opt: 732, E(): 5.9e-23; 44.4% identity in 266 aa overlap. Also similar to several Mycobacterium tuberculosis STPK proteins e.g. Rv0014c|PKNB, Rv0015c|PKNA, Rv1743|PKNE, Rv1266c|PKNH etc. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases.; Probable transmembrane serine/threonine-protein kinase L PknL (protein kinase L) (STPK L) 2439140 pknL 888340 pknL Mycobacterium tuberculosis H37Rv Probable transmembrane serine/threonine-protein kinase L PknL (protein kinase L) (STPK L) NP_216692.1 2437941 D 83332 CDS NP_216693.1 15609314 888326 complement(2439282..2439947) 1 NC_000962.3 Rv2177c, (MTV021.10c), len: 221 aa. Possible IS1558 transposase (see citation below), similar to several is element proteins and transposases but nearly identical to last 221 residues of MTCY428_23 (333 aa). FASTA scores: Z81451|MTCY428_23 Mycobacterium tuberculosis cosmid (333 aa) opt: 1491, E() : 0; 98.6% identity in 221 aa overlap.; Possible transposase 2439947 888326 Rv2177c Mycobacterium tuberculosis H37Rv Possible transposase NP_216693.1 2439282 R 83332 CDS NP_216694.1 15609315 888309 complement(2440332..2441720) 1 NC_000962.3 Rv2178c, (MTV021.11c), len: 462 aa. aroG,3-deoxy-D-arabino-heptulosonate 7-phosphate synthase similar to many, especially those from plants. FASTA scores: Y15113|M C3DDAH7P_1Morinda citrifolia mRNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (535 aa) opt: 1421, E(): 0; 48.3% identity in 443 aa overlap.; 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroG (DAHP synthetase, phenylalanine-repressible) 2441720 aroG 888309 aroG Mycobacterium tuberculosis H37Rv 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroG (DAHP synthetase, phenylalanine-repressible) NP_216694.1 2440332 R 83332 CDS NP_216695.1 15609316 887927 complement(2441811..2442317) 1 NC_000962.3 Rv2179c, (MTV021.12c), len: 168 aa. Conserved hypothetical protein, equivalent to conserved hypothetical protein from Mycobacterium leprae ML0895 conserved hypothetical protein (171 aa). FASTA scores: opt: 977, E(): 1.4e-58; 82.530% identity in 166 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2442317 887927 Rv2179c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216695.1 2441811 R 83332 CDS NP_216696.1 15609317 887954 complement(2442327..2443214) 1 NC_000962.3 Rv2180c, (MTV021.13c), len: 295 aa. Probable conserved integral membrane protein, similar to pir||T35292 probable integral membrane protein from Streptomyces coelicolor >gi|5578858|emb|CAB51260.1| (AL096872) (246 aa) (36% identity in 249 aa overlap).; Probable conserved integral membrane protein 2443214 887954 Rv2180c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216696.1 2442327 R 83332 CDS NP_216697.1 15609318 888269 2443302..2444585 1 NC_000962.3 Rv2181, (MTV021.14), len: 427 aa. Alpha(1->2)mannosyltransferase (See Kaur et al., 2006). Probable integral membrane protein, similar to others in Mycobacterium tuberculosis e.g. Rv1159 (MTCI65.26, 431 aa). Start uncertain. FASTA scores: Z95584|MTCI65_26 (431 aa) opt: 428, E(): 8e-22; 31.2% identity in 407 aa overlap. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003).; Alpha(1->2)mannosyltransferase 2444585 888269 Rv2181 Mycobacterium tuberculosis H37Rv Alpha(1->2)mannosyltransferase NP_216697.1 2443302 D 83332 CDS NP_216698.1 15609319 888625 complement(2444586..2445329) 1 NC_000962.3 Rv2182c, (MTV021.15c), len: 247 aa. Probable 1-acylglycerol-3-phosphate O-acyltransferase, similar to many e.g. in Streptomyces. Contains PS00017 ATP/GTP-binding site motif A (P-loop). FASTA scores: pir||T35503 1-acylglycerol-3-phosphate O-acyltransferase homolog SC6E10.16c - Streptomyces coelicolor >gi|5689932|emb|CAB51970.1| (AL109661) hypothetical protein [Streptomyces coelicolor A3(2)] Length = 262, Expect = 6e-61 (54% identity in 215 aa overlap).; 1-acylglycerol-3-phosphate O-acyltransferase 2445329 888625 Rv2182c Mycobacterium tuberculosis H37Rv 1-acylglycerol-3-phosphate O-acyltransferase NP_216698.1 2444586 R 83332 CDS NP_216699.1 15609320 887650 complement(2445415..2445810) 1 NC_000962.3 Rv2183c, (MTV021.16c), len: 131 aa. Conserved protein, equivalent to Mycobacterium leprae hypothetical protein ML0891 (MLCB268.25c, 130 aa). FASTA scores: opt: 558, E(): 8.3e-28; 61.832% identity in 131 aa overlap >gi|13092963|emb|CAC31272.1| (AL583920) (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2445810 887650 Rv2183c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216699.1 2445415 R 83332 CDS NP_216700.1 15609321 888152 complement(2445807..2446946) 1 NC_000962.3 Rv2184c, (MTV021.17c), len: 379 aa. Conserved hypothetical protein, equivalent to hypothetical protein ML0890 (415 aa) from Mycobacterium leprae and also shows some similarity to other hypothetical proteins. FASTA scores: ML0890 opt: 1949; 79.630% identity in 378 aa overlap >emb|CAA18692.1| (AL022602) >gi|13092962|emb|CAC31271.1| (AL583920) and sptr|Q55794|Q55794 hypothetical 44.6 kDa protein. (396 aa) opt: 251, E(): 3.3e-09; 25.5% identity in 384 aa overlap.; hypothetical protein 2446946 888152 Rv2184c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216700.1 2445807 R 83332 CDS NP_216701.1 15609322 887239 complement(2447066..2447500) 1 NC_000962.3 Rv2185c, (MTV021.18c), len: 144 aa. TB16.3,conserved protein, similar to other hypothetical actinomycete proteins and equivalent to Mycobacterium leprae ML0889 (144 aa). Some similarity to Mycobacterium tuberculosis Rv0854, Rv0856, Rv0857, Rv0164 and other Mycobacterium leprae proteins. FASTA scores : ML0889 opt: 811; 85.417% identity in 144 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Conserved protein TB16.3 2447500 TB16.3 887239 TB16.3 Mycobacterium tuberculosis H37Rv Conserved protein TB16.3 NP_216701.1 2447066 R 83332 CDS NP_216702.1 15609323 887347 complement(2447605..2447994) 1 NC_000962.3 Rv2186c, (MTV021.19c), len: 129 aa. Conserved hypothetical protein, equivalent to hypothetical Mycobacterium leprae protein ML0888 (135 aa). FASTA scores: ML0888 opt: 704, E(): 2.9e-43; 80.000% identity in 130 aa overlap CAA18694.1| (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 2447994 887347 Rv2186c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216702.1 2447605 R 83332 CDS NP_216703.1 15609324 887456 2448160..2449962 1 NC_000962.3 Rv2187, (MTV021.20), len: 600 aa. fadD15,long-chain-fatty-acid-CoA ligase, similar to several e.g. P44446|LCFH_HAEIN putative long-chain-fatty-acid--CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: (607 aa) opt: 992, E(): 0, (31.5% identity in 578 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Long-chain-fatty-acid-CoA ligase FadD15 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 2449962 fadD15 887456 fadD15 Mycobacterium tuberculosis H37Rv Long-chain-fatty-acid-CoA ligase FadD15 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_216703.1 2448160 D 83332 CDS NP_216704.2 57116954 887278 complement(2449993..2451150) 1 NC_000962.3 Rv2188c, (MTV021.21c), len: 385 aa. PimB (previously known as pimB'), mannosyltransferase. Equivalent to Mycobacterium leprae ML0886 putative glycosyl transferase (384 aa). FASTA scores: ML0886 (CAA18697.1| (AL022602) ) opt: 2113, E(): 1.8e-106; 81.462% identity in 383 aa overlap; sptr|P73369|P73369 hypothetical 46.2 kDa protein (404 aa) opt: 379, E(): 2.2e-18; 27.5% identity in 397 aa overlap. Start changed since first submission, now 14 aa shorter.; Mannosyltransferase PimB 2451150 pimB 887278 pimB Mycobacterium tuberculosis H37Rv Mannosyltransferase PimB NP_216704.2 2449993 R 83332 CDS NP_216705.1 15609326 887575 complement(2451247..2452020) 1 NC_000962.3 Rv2189c, (MTV021.22c), len: 257 aa. Conserved hypothetical protein; some similarity to hypothetical protein SC6G10.07c (385 aa) from Streptomyces coelicolor A3(2). Smith-Waterman scores: pir||T35516 hypothetical protein SC6G10.07c - Streptomyces coelicolor >gi|4539203|emb|CAB39861.1| (AL049497) Expect = 2e-08; 30% identity in 245 aa overlap.; hypothetical protein 2452020 887575 Rv2189c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216705.1 2451247 R 83332 CDS NP_216706.1 15609327 888670 complement(2452115..2453272) 1 NC_000962.3 Rv2190c, (MTV021.23c, MTCY190.01c), len: 385 aa. Conserved hypothetical protein; similar to other hypothetical mycobacterial proteins, including Rv1477,Rv1478, Rv1566c, Rv0024, that are similar to protein p60 precursors from Listeria e.g. Q018 38|P60_LISSE protein p60 precursor (invasion-associated protein) (524 aa). FASTA scores: gp|Z80233|MTCY10H4_25 (281 aa) opt: 290, E(): 6.9e-05; 37.0% identity in 127 aa overlap and sp|Q01838|P60_LISSE protein P60 precursor (523 aa) opt: 268, E(): 0.00071; 38.5% identity in 104 aa overlap.; hypothetical protein 2453272 888670 Rv2190c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216706.1 2452115 R 83332 CDS NP_216707.1 15609328 887265 2453819..2455756 1 NC_000962.3 Rv2191, (MTCY190.02), len: 645 aa. Conserved hypothetical protein, similar to SW:DP3A_B ACSU P13267 DNA polymerase III, alpha chain (31.3% identity in 249 aa overlap) and SW:UVRC_ECOLI P07028 excinuclease ABC subunit C (25.7% identity in 230 aa overlap). Also similar to M. tuberculosis Rv3711c (dnaQ DNA polymerase III e chain) and Rv1420 (uvrC excinuclease ABC subunit C); hypothetical protein 2455756 887265 Rv2191 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216707.1 2453819 D 83332 CDS NP_216708.1 15609329 887681 complement(2455631..2456743) 1 NC_000962.3 Rv2192c, (MTCY190.03c), len: 370 aa. Probable trpD,anthranilate phosphoribosyltransferase (see citation below), similar to e.g. TRPD_LACCA|P17170, (43.2% identity in 308 aa overlap). Initiation codon uncertain, gtg at 4086 in MTCY190 favoured by homology but this has no clear ribosome binding site.; Probable anthranilate phosphoribosyltransferase TrpD 2456743 trpD 887681 trpD Mycobacterium tuberculosis H37Rv Probable anthranilate phosphoribosyltransferase TrpD NP_216708.1 2455631 R 83332 CDS NP_216709.1 15609330 887425 2456901..2457512 1 NC_000962.3 Rv2193, (MTCY190.04), len: 203 aa. Probable ctaE,cytochrome c oxidase polypeptide III (cox3), with strong similarity to others e.g. COX3_SYNY3|Q06475 (29.8% identity in 225 aa overlap).; Probable cytochrome C oxidase (subunit III) CtaE 2457512 ctaE 887425 ctaE Mycobacterium tuberculosis H37Rv Probable cytochrome C oxidase (subunit III) CtaE NP_216709.1 2456901 D 83332 CDS NP_216710.1 15609331 888737 2457553..2458395 1 NC_000962.3 Rv2194, (MTCY190.05), len: 280 aa. Probable qcrC,Ubiquinol-cytochrome C reductase cytochrome C subunit (cyoA), shows similarity to cytochrome c family; contains 2 X PS00190 Cytochrome c family heme-binding site signature.; Probable ubiquinol-cytochrome C reductase QcrC (cytochrome C subunit) 2458395 qcrC 888737 qcrC Mycobacterium tuberculosis H37Rv Probable ubiquinol-cytochrome C reductase QcrC (cytochrome C subunit) NP_216710.1 2457553 D 83332 CDS NP_216711.1 15609332 888420 2458392..2459681 1 NC_000962.3 Rv2195, (MTCY190.06), len: 429 aa. Probable qcrA,Ubiquinol-cytochrome C reductase iron-sulfur subunit (cyoB), shows some similarity to cytochrome B6-F complex iron-sulphur subunits (Rieske iron-sulfur protein); contains PS00200 Rieske iron-sulfur protein signature 2; Probable rieske iron-sulfur protein QcrA 2459681 qcrA 888420 qcrA Mycobacterium tuberculosis H37Rv Probable rieske iron-sulfur protein QcrA NP_216711.1 2458392 D 83332 CDS NP_216712.1 15609333 887400 2459678..2461327 1 NC_000962.3 Rv2196, (MTCY190.07), len: 549 aa. Probable qcrB,Ubiquinol-cytochrome C reductase cytochrome B subunit (cytB), integral membrane protein, low similarity in amino-terminal half to cytochrome b subunits, highly similar at C-terminus to SW:12KD_MYCLE P15878 12 KD protein PIR:S08427 (86.9% identity in 153 aa overlap). FASTA scores: sp|Q45658|QCRB_BACST menaquinol-cytochrome C reductase (224 aa) opt: 341, E(): 6.8e-15; 28.0% identity in 207 aa overlap; Probable ubiquinol-cytochrome C reductase QcrB (cytochrome B subunit) 2461327 qcrB 887400 qcrB Mycobacterium tuberculosis H37Rv Probable ubiquinol-cytochrome C reductase QcrB (cytochrome B subunit) NP_216712.1 2459678 D 83332 CDS NP_216713.1 15609334 887213 complement(2461504..2462148) 1 NC_000962.3 Rv2197c, (MTCY190.08c), len: 214 aa. Probable conserved transmembrane protein, equivalent to ML0878 conserved hypothetical protein (212 aa) of Mycobacterium leprae. FASTA scores: opt: 858; 62.559% identity in 211 aa overlap CAC31259.1|(AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved transmembrane protein 2462148 887213 Rv2197c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216713.1 2461504 R 83332 CDS NP_216714.1 15609335 887471 complement(2462148..2463047) 1 NC_000962.3 Rv2198c, (MTCY190.09c), len: 299 aa. Probable mmpS3,conserved membrane protein (see citation below), equivalent to ML0877|mmpS3 putative membrane protein from Mycobacterium leprae (293 aa), FASTA scores: opt: 1089,E(): 1.2e-43, (69.80% identity in 308 aa overlap). Also similar to other proteins e.g. Rv3209 from Mycobacterium tuberculosis. Contains PS00499 C2 domain signature, a hydrophobic region, and a repetitive proline and threonine rich region. Belongs to the MmpS family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved membrane protein MmpS3 2463047 mmpS3 887471 mmpS3 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein MmpS3 NP_216714.1 2462148 R 83332 CDS NP_216715.1 15609336 888692 complement(2463233..2463652) 1 NC_000962.3 Rv2199c, (MTCY190.10c), len: 139 aa. Possible conserved integral membrane protein, similar to hypothetical membrane proteins in Actinomycetes and equivalent to Mycobacterium leprae, ML0876, putative membrane protein (139 aa) FASTA scores: opt: 866, E(): 1.1e-43; 91.367% identity in 139 aa overlap CAC31257.1| (AL583920); Possible conserved integral membrane protein 2463652 888692 Rv2199c Mycobacterium tuberculosis H37Rv Possible conserved integral membrane protein NP_216715.1 2463233 R 83332 CDS NP_216716.1 15609337 888799 complement(2463660..2464751) 1 NC_000962.3 Rv2200c, (MTCY190.11c), len: 363 aa. Probable ctaC,transmembrane cytochrome C oxidase (subunit II), COX2,similar e.g. to JT0964 cytochrome-c oxidase chain II (23.0% identity in 317 aa overlap); etc. Contains PS00078 Cytochrome c oxidase subunit II, copper a binding region signature. Belongs to the cytochrome C oxidase subunit 2 family.; Probable transmembrane cytochrome C oxidase (subunit II) CtaC 2464751 ctaC 888799 ctaC Mycobacterium tuberculosis H37Rv Probable transmembrane cytochrome C oxidase (subunit II) CtaC NP_216716.1 2463660 R 83332 CDS NP_216717.1 15609338 888472 2464997..2466955 1 NC_000962.3 Rv2201, (MTCY190.12), len: 652 aa. Probable asnB,asparagine synthetase, similar to e.g. SW:ASNH_BACSU P42113 putative asparagine synthetase (26.0% identity in 438 aa overlap); Probable asparagine synthetase AsnB 2466955 asnB 888472 asnB Mycobacterium tuberculosis H37Rv Probable asparagine synthetase AsnB NP_216717.1 2464997 D 83332 CDS NP_216718.1 15609339 888551 complement(2467053..2468027) 1 NC_000962.3 Rv2202c, (MTCY190.13c), len: 324 aa. AdoK, Adenosine kinase activity proven biochemically (See Long et al. 2003). Similar to several others but shows greater sequence homology with ribokinase and fructokinase than it does with other AKs e.g. AE000915_1 Methanobacterium thermoautotrop (309 aa) FASTA score: opt: 370, E(): 3.3e-18; 31.2% identity in 276 aa overlap. Low similarity to carbohydrate kinases, e.g. SW:RBSK_BACSU P36945 ribokinase (23.9% identity in 272 aa overlap); contains PS00583 pfkB family of carbohydrate kinases signature 1. Previously known as cbhK; Adenosine kinase 2468027 adoK 888551 adoK Mycobacterium tuberculosis H37Rv Adenosine kinase NP_216718.1 2467053 R 83332 CDS NP_216719.1 15609340 888318 2468231..2468923 1 NC_000962.3 Rv2203, (MTCY190.14), len: 230 aa. Possible conserved membrane protein; has single hydrophobic stretch from aa 75 to 97 and is equivalent to Mycobacterium leprae ML0872 putative membrane protein (171 aa). FASTA scores: opt: 821, E(): 3.4e-42; 72.353% identity in 170 aa overlap - CAC31253.1| (AL583920). 2468411. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved membrane protein 2468923 888318 Rv2203 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_216719.1 2468231 D 83332 CDS NP_216720.1 15609341 888428 complement(2468931..2469287) 1 NC_000962.3 Rv2204c, (MTCY190.15c), len: 118 aa. Conserved protein. Similar to conserved hypothetical proteins in Actinomycetes and equivalent to Mycobacterium leprae ML0871|ML0871 conserved hypothetical protein (118 aa) and to sp|P45344|YADR_HAEIN hypothetical protein HI1723 (114 aa). FASTA score: ML0871 opt: 720, E(): 8.4e-45; 92.373% identity in 118 aa overlapCAC31252.1| (AL583920); and P45344 opt: 346, E(): 1.8e-18; 45.6% identity in 103 aa overlap. Contains PS01152 Hypothetical hesB/y yadR/yfhF family signature; hypothetical protein 2469287 888428 Rv2204c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216720.1 2468931 R 83332 CDS NP_216721.2 57116955 887277 complement(2469387..2470463) 1 NC_000962.3 Rv2205c, (MTCY190.16c), len: 358 aa. Conserved hypothetical protein. Very similar to YHAD_ECOLI|P23524 hypothetical protein (YHAD (E.coli) / YXAA (S14A) (B.subtilis) family) (41.6% identity in 154 aa overlap),and to other members of the glycerate kinase family. Start changed since first submission; protein now 122 aa shorter,owing to extension of Rv2206. Nucleotide position 2470149 in the genome sequence has been corrected, T:C resulting in E105E.; hypothetical protein 2470463 887277 Rv2205c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216721.2 2469387 R 83332 CDS NP_216722.2 57116956 888107 2470622..2471332 1 NC_000962.3 Rv2206, (MTCY190.17), len: 236 aa. Probable conserved transmembrane protein. Equivalent to hypothetical protein ML0869 (247 aa) of Mycobacterium leprae gZ98741|MLCB22_2 (247 aa), FASTA scores: opt: 1052, (67.5% identity in 237 aa overlap). Two hydrophobic stretches in C-terminal part. Start changed since original submission (+112 aa).; Probable conserved transmembrane protein 2471332 888107 Rv2206 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216722.2 2470622 D 83332 CDS NP_216723.1 15609344 887781 2471411..2472496 1 NC_000962.3 Rv2207, (MTCY190.18), len: 361 aa. Probable cobT,phosphoribosyltransferase, similar to many e.g. SW:COBT_ECOLI P36562 nicotinate-nucleotide--dimethylbenzimidazol phosphoribosyltransferase (34.6% identity in 341 aa overlap); Probable nicotinate-nucleotide-dimethylbenzimidazol phosphoribosyltransferase CobT 2472496 cobT 887781 cobT Mycobacterium tuberculosis H37Rv Probable nicotinate-nucleotide-dimethylbenzimidazol phosphoribosyltransferase CobT NP_216723.1 2471411 D 83332 CDS NP_216724.1 15609345 887572 2472493..2473242 1 NC_000962.3 Rv2208, (MTCY190.19), len: 249 aa. Probable cobS,cobalamin 5'-phosphate synthase; similarity to SW:COBS_ECOLI P36561 cobalamin (5'-phosphate) synthase (28.0% identity in 243 aa overlap); Probable cobalamin 5'-phosphate synthase CobS 2473242 cobS 887572 cobS Mycobacterium tuberculosis H37Rv Probable cobalamin 5'-phosphate synthase CobS NP_216724.1 2472493 D 83332 CDS NP_216725.1 15609346 887230 2473400..2474938 1 NC_000962.3 Rv2209, (MTCY190.20), len: 512 aa. Probable conserved integral membrane protein, similar to but longer than Rv0246 gp|AL021929|MTV 034_12 Mycobacterium tuberculosis (436 aa). FASTA score: opt: 712, E(): 2.8e-32; 33.4% identity in 422 aa overlap; Probable conserved integral membrane protein 2474938 887230 Rv2209 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216725.1 2473400 D 83332 CDS NP_216726.1 15609347 888352 complement(2474864..2475970) 1 NC_000962.3 Rv2210c, (MTCY190.21c), len: 368 aa. ilvE,Branched-chain-amino-acid transaminase, highly similar to many e.g. YWAA_BACSU|P39576 from Bacillus subtilis (48.4% identity in 339 aa overlap); etc.; Branched-chain amino acid transaminase IlvE 2475970 ilvE 888352 ilvE Mycobacterium tuberculosis H37Rv Branched-chain amino acid transaminase IlvE NP_216726.1 2474864 R 83332 CDS NP_216727.1 15609348 887233 complement(2476042..2477181) 1 NC_000962.3 Rv2211c, (MTCY190.22), len: 379 aa. Probable gcvT,aminomethyltransferase, similar to many e.g. GCST_ECOLI|P27248 for Escherichia coli (38.2% identity in 364 aa overlap); etc. Belongs to the GcvT family.; Probable aminomethyltransferase GcvT (glycine cleavage system T protein) 2477181 gcvT 887233 gcvT Mycobacterium tuberculosis H37Rv Probable aminomethyltransferase GcvT (glycine cleavage system T protein) NP_216727.1 2476042 R 83332 CDS NP_216728.1 15609349 887979 2477190..2478326 1 NC_000962.3 Rv2212, (MTCY190.23), len: 378 aa. Adenylyl cyclase (See Abdel Motaal et al., 2006). Some similarity to e.g. SW:CYAA_STRCO P40135 adenylate cyclase (29.2% identity in 291 aa overlap); ttg at 24614 in MTCY190 has a better rbs. Contains possible helix-turn-helix motif at aa 64-85,(+2.72 SD). Also similar to Rv1264 and Rv1647; Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) 2478326 887979 Rv2212 Mycobacterium tuberculosis H37Rv Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) NP_216728.1 2477190 D 83332 CDS NP_216729.1 15609350 888105 2478338..2479885 1 NC_000962.3 Rv2213, (MTCY190.24), len: 515 aa. Probable pepB,leucine aminopeptidase, similar to many e.g. SW:AMPA_ECOLI P11648 aminopeptidase a/I, (41.4% identity in 309 aa overlap). Equivalent to Z98741|MLCB22_6 Mycobacterium leprae cosmid B22; Am (524 aa), FASTA scores: opt: 2793,E(): 0; 83.1% identity in 522 aa overlap. Contains PS00631 Cytosol aminopeptidase signature, ntdaegrl. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable aminopeptidase PepB 2479885 pepB 888105 pepB Mycobacterium tuberculosis H37Rv Probable aminopeptidase PepB NP_216729.1 2478338 D 83332 CDS NP_216730.1 15609351 887472 complement(2479923..2481701) 1 NC_000962.3 Rv2214c, (MTCY190.25c), len: 592 aa. Possible ephD,short-chain dehydrogenase (see citation below), equivalent to Z98741|MLCB22_8 Mycobacterium leprae cosmid B22; (596 aa), FASTA score: opt: 3262, E(): 0; 80.4% identity in 596 aa overlap. C-terminus similar to short-chain alcohol dehydrogenase family, similar to SW:LIGD_PSEPA Q01198 c alpha-dehydrogenase (30.7% identity in 241 aa overlap); contains PS00061 Short-chain alcohol dehydrogenase family signature, PS00697 ATP-dependent DNA ligase AMP-binding site. N-terminus corresponds to several epoxide hydrolases of plants and Mycobacterium tuberculosis e.g. MTCY9F925; Possible short-chain dehydrogenase EphD 2481701 ephD 887472 ephD Mycobacterium tuberculosis H37Rv Possible short-chain dehydrogenase EphD NP_216730.1 2479923 R 83332 CDS NP_216731.1 15609352 888777 2481965..2483626 1 NC_000962.3 Rv2215, (MTCY190.26), len: 553 aa. DlaT,dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase, proven biochemically (see Tian et al. 2005),similar to e.g. SW:O PD2_ACHLA P35489 dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex (35.3% identity in 552 aa overlap); contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. Rhodanine compounds inhibit DlaT|Rv2215 and can kill non-replicating mycobacteria in mouse bone marrow-derived macrophages (See Bryk et al.,2008). LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011).; DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase 2483626 dlaT 888777 dlaT Mycobacterium tuberculosis H37Rv DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase NP_216731.1 2481965 D 83332 CDS NP_216732.1 15609353 887640 2483626..2484531 1 NC_000962.3 Rv2216, (MTCY190.27), len: 301 aa. Conserved protein, equivalent to Mycobacterium leprae ML0860 (307 aa), Z98741|MLCB22_10 Mycobacterium leprae cosmid B22; H (307 aa). FASTA score: opt: 1656, E(): 0; 84.2% identity in 297 aa overlap. Also gp|AE000319|ECAE000319_8 Escherichia coli strain K12 MG1655 (297 aa) opt: 640, E(): 0; 39.5% identity in 294 aa overlap.; hypothetical protein 2484531 887640 Rv2216 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216732.1 2483626 D 83332 CDS NP_216733.1 15609354 887626 2484584..2485276 1 NC_000962.3 Rv2217, (MTCY190.28), len: 230 aa. Probable lipB,similar to SW:LIPB_ECOLI P30976 liopate biosynthesis protein B (33.8% identity in 160 aa overlap). Equivalent to gp|Z98741| MLCB22_11 Mycobacterium leprae (235 aa). FASTA score: opt: 1124, E(): 0; 78.4% identity in 218 aa overlap; Probable lipoate biosynthesis protein B LipB 2485276 lipB 887626 lipB Mycobacterium tuberculosis H37Rv Probable lipoate biosynthesis protein B LipB NP_216733.1 2484584 D 83332 CDS NP_216734.1 15609355 887922 2485273..2486208 1 NC_000962.3 Rv2218, (MTCY190.29), len: 311 aa. Probable lipA,lipoic acid synthetase, similar to e.g. SW:LIPA_HAEIN P44463 (42.6% identity in 291 aa overlap). Equivalent to Z98741|MLCB2 2_12 Mycobacterium leprae cosmid B22; (314 aa). FASTA score : opt: 1836, E(): 0; 86.8% identity in 310 aa overlap; Probable lipoate biosynthesis protein A LipA 2486208 lipA 887922 lipA Mycobacterium tuberculosis H37Rv Probable lipoate biosynthesis protein A LipA NP_216734.1 2485273 D 83332 CDS NP_216735.1 15609356 888380 2486235..2486987 1 NC_000962.3 Rv2219, (MTCY190.30), len: 250 aa. Probable conserved transmembrane protein. Equivalent to hypothetical membrane protein ML0857 (250 aa) from Mycobacterium leprae Z98741 |MLCB22_13 Mycobacterium leprae cosmid B22; H (250 aa) opt : 1328, E(): 0; 80.8% identity in 250 aa overlap.; Probable conserved transmembrane protein 2486987 888380 Rv2219 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216735.1 2486235 D 83332 CDS YP_177661.1 57116957 3205099 complement(2486994..2487416) 1 NC_000962.3 Rv2219A, len: 140 aa. Probable conserved membrane protein, similar to SC3H12.05c|AL355740_5 possible integral membrane protein from Streptomyces coelicolor (155 aa),FASTA scores: opt: 327, E(): 7.5e-14, (46.6% identity in 133 aa overlap), also linked to glnA.; Probable conserved membrane protein 2487416 3205099 Rv2219A Mycobacterium tuberculosis H37Rv Probable conserved membrane protein YP_177661.1 2486994 R 83332 CDS NP_216736.1 15609357 888383 2487615..2489051 1 NC_000962.3 Rv2220, (MTCY190.31, MTCY427.01), len: 478 aa. glnA1, glutamine synthetase class I (see Tullius et al.,2001), similar to many e.g. GLNA_STRCO|P15106 from Streptomyces coelicolor, FASTA score: (71.4% identity in 475 aa overlap); etc. Also similar to three other potential glutamine synthetases in Mycobacterium tuberculosis: Rv2222c|glnA2, Rv2860c|glnA4, and Rv1878|glnA3. Contains PS00180 Glutamine synthetase signature 1, PS00181 Glutamine synthetase putative ATP-binding region signature, and PS00182 Glutamine synthetase class-I adenylation site. Belongs to the glutamine synthetase family. Note has shown to be essential for M. tuberculosis virulence.; Glutamine synthetase GlnA1 (glutamine synthase) (GS-I) 2489051 glnA1 888383 glnA1 Mycobacterium tuberculosis H37Rv Glutamine synthetase GlnA1 (glutamine synthase) (GS-I) NP_216736.1 2487615 D 83332 CDS NP_216737.1 15609358 887995 complement(2489369..2492353) 1 NC_000962.3 Rv2221c, (MTCY190.32c, MTCY427.02c), len: 994 aa. glnE, glutamate-ammonia-ligase adenylyltransferase (see citations below), similar to others e.g. GLNE_ECOLI|P30870 glutamate-ammonia-ligase adenylyltransferase from Escherichia coli, FASTA score: (24.4% identity in 721 aa overlap); GLNE_HAEIN|P44419 Glutamate-ammonia-ligase adenylyltransferase from Haemophilus influenzae (981 aa),FASTA score: (28.1% identity in 199 aa overlap); etc. Note that initiation codon uncertain.; Glutamate-ammonia-ligase adenylyltransferase GlnE (glutamine-synthetase adenylyltransferase) 2492353 glnE 887995 glnE Mycobacterium tuberculosis H37Rv Glutamate-ammonia-ligase adenylyltransferase GlnE (glutamine-synthetase adenylyltransferase) NP_216737.1 2489369 R 83332 CDS NP_216738.1 15609359 888238 complement(2492402..2493742) 1 NC_000962.3 Rv2222c, (MTCY427.03c), len: 446 aa. Probable glnA2,glutamine synthetase class II, similar to others. Also similar to three other potential glutamine synthetases in Mycobacterium tuberculosis: Rv2220|glnA1, Rv2860c|glnA4,and Rv1878|glnA3. Belongs to the glutamine synthetase family.; Probable glutamine synthetase GlnA2 (glutamine synthase) (GS-II) 2493742 glnA2 888238 glnA2 Mycobacterium tuberculosis H37Rv Probable glutamine synthetase GlnA2 (glutamine synthase) (GS-II) NP_216738.1 2492402 R 83332 CDS NP_216739.1 15609360 888093 complement(2493837..2495399) 1 NC_000962.3 Rv2223c, (MTCY427.04c), len: 520 aa. Probable exported protease ; has signal sequence. Very similar to three proteases/peptidases from Streptomyces spp.: L42758,L42759, L27466. FASTA score: L42758|STMSLPD STMSLPD NID: g940302 - Streptomyces (539 aa) opt: 1032 E(): 0, (37.5% identity in 533 aa overlap). Also similar to hypothetical proteins YZZE _ECOLI|P34211 from Escherichia coli (25.4% identity in 406 aa overlap) and PIR:B36944 in ompP 3' region (27.5% identity in 218 aa overlap). Highly similar to Rv2224c and Rv2672 (49.3% identity in 507 aa overlap); contains PS00120 Lipases, serine active site. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable exported protease 2495399 888093 Rv2223c Mycobacterium tuberculosis H37Rv Probable exported protease NP_216739.1 2493837 R 83332 CDS NP_216740.1 15609361 887857 complement(2495461..2497023) 1 NC_000962.3 Rv2224c, (MTCY427.05c), len: 520 aa. Probable caeA,carboxylesterase; has signal sequence and lipoprotein motif at N-terminal end. Very similar to three proteases/peptidases from Streptomyces spp.: L42758,L42759, L27466. FASTA score: L4 2758|STMSLPD STMSLPD NID: g940302 - Streptomyces (539 aa) opt: 1032 E(): 0, (37.5% identity in 533 aa overlap). Similar to hypothetical protein SW:YZZE_ECOLI P34211 (27.7% identity in 412 aa overlap) and highly similar to Rv2224c and Rv2672 (49.3% identity in 507 aa overlap); contains PS00013, Prokaryotic membrane lipoprotein lipid attachment site, and PS00120 Lipases, serine active site. Conserved in M. tuberculosis,M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Predicted to be an outer membrane protein (See Song et al.,2008).; Probable carboxylesterase CaeA 2497023 caeA 887857 caeA Mycobacterium tuberculosis H37Rv Probable carboxylesterase CaeA NP_216740.1 2495461 R 83332 CDS NP_216741.1 15609362 887440 2497742..2498587 1 NC_000962.3 Rv2225, (MTCY427.06), len: 281 aa. panB,3-methyl-2-oxobutanoate hydroxymethyltransferase, similar to PANB_ECOLI|P31057 3-methyl-2-oxobutanoate hydroxymethyltransferase from Escherichia coli (45.9% identity in 257 aa overlap). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006).; 3-methyl-2-oxobutanoate hydroxymethyltransferase PanB 2498587 panB 887440 panB Mycobacterium tuberculosis H37Rv 3-methyl-2-oxobutanoate hydroxymethyltransferase PanB NP_216741.1 2497742 D 83332 CDS NP_216742.1 15609363 888513 2498832..2500373 1 NC_000962.3 Rv2226, (MTCY427.07), len: 513 aa. Conserved protein, similar to hypothetical secreted protein (510 aa) from Streptomyces coelicolor A3(2) emb|CAB59601.1| (AL132662) hypothetical secreted protein [Streptomyces coelicolor. Smith-Waterman scores Expect = 5e-44 Identities = 166/506 (32%); hypothetical protein 2500373 888513 Rv2226 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216742.1 2498832 D 83332 CDS NP_216743.1 15609364 888581 2500931..2501632 1 NC_000962.3 Rv2227, (MTCY427.08), len: 233 aa. Conserved hypothetical protein, similar to conserved hypothetical proteins from various bacteria e.g. gb|AAK22693.1| (AE005746) conserved hypothetical protein from Caulobacter crescentus (234 aa) Smith-Waterman score = 109 bits (429),Expect = 1e-41 Identities = 83/167 (49%); hypothetical protein 2501632 888581 Rv2227 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216743.1 2500931 D 83332 CDS NP_216744.1 15609365 888108 complement(2501644..2502738) 1 NC_000962.3 Rv2228c, (MTCY427.09c), len: 364 aa. Multifunctional protein with RNase H, alpha-ribazole phosphatase, and acid phosphatase activities. Some similarity to phosphoglycerate mutase and ribonuclease H. Similar to CAB88177.1|AL352972 putative bifunctional protein (ribonuclease H/phosphoglycerate mutase) from Streptomyces coelicolor A3(2) (497 aa); Smith-Waterman scores: 107 bits (424),Expect = 4e-41 Identities = 160/485 (32%). Also similar in C-terminal part to Rv2419c and Rv2135c.; Multifunctional protein Has RNASE H,alpha-ribazole phosphatase, and acid phosphatase activities 2502738 888108 Rv2228c Mycobacterium tuberculosis H37Rv Multifunctional protein Has RNASE H,alpha-ribazole phosphatase, and acid phosphatase activities NP_216744.1 2501644 R 83332 CDS NP_216745.1 15609366 888264 complement(2502735..2503472) 1 NC_000962.3 Rv2229c, (MTCY427.10c), len: 245 aa. Conserved protein; probable coiled-coil protein similar to conserved hypothetical proteins in Actinomycetes. Equivalent to Mycobacterium leprae ML1638 (232 aa), FASTA scores: opt: 868 E(): 4.4e-43; 60.870% identity in 230 aa overlap emb|CAC30589.1| (AL583922). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 2503472 888264 Rv2229c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216745.1 2502735 R 83332 CDS NP_216746.1 15609367 888231 complement(2503469..2504608) 1 NC_000962.3 Rv2230c, (MTCY427.11c), len: 379 aa. Conserved protein. Equivalent to Mycobacterium leprae, ML1639,conserved hypothetical protein (385 aa). Similar to hypothetical proteins from B. subtilis, P54472, and L. monocytogenes, P53434. FASTA score: ML1639 (MLCB1243.36) opt: 2088, E(): 4e-107; 79.481% identity in 385 aa overlap same as >pir||T44719 hypothetical protein MLCB1243.36 [imported] - Mycobacterium leprae >gi|3150237|emb|CAA19217.1| (AL023635); P54472|YQFO_BACSU hypothetical 30. 7 kDa protein in (279 aa) opt: 604; E(): 2.2e-30; 38.8% identity in 258 aa overlap. P53434|YRP2_LISMO hypothetical 41.4 kDa protein (373 aa) opt: 595, E(): 1e-29; 30.7% identity in 326 aa overlap; hypothetical protein 2504608 888231 Rv2230c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216746.1 2503469 R 83332 CDS NP_216747.1 15609368 888337 complement(2504605..2505699) 1 NC_000962.3 Rv2231c, (MTCY427.12c), len: 364 aa. Possible cobC,aminotransferase. Note that initiation codon uncertain. Similar to CobC aminotransferases e.g. sp|P21633|COBC_PSEDE COBC protein (333 aa) opt: 277, E(): 1.7e-11; 28.8% identity in 313 aa overlap and also to e.g. SW:HIS8_ECOLI P06986 histidinol-phosphate aminotransferase (27.0% identity in 289 aa overlap), contains PS00105 aminotransferases class-I pyridoxal-phosphate attachment site. Real Mycobacterium tuberculosis histidinol-phosphate aminotransferase, hisC, is Rv1600 (MTCY336.04c).; Possible aminotransferase CobC 2505699 cobC 888337 cobC Mycobacterium tuberculosis H37Rv Possible aminotransferase CobC NP_216747.1 2504605 R 83332 CDS YP_007410917.1 448817044 14515883 complement(2505736..2506161) 1 NC_000962.3 Rv2231A, len: 141 aa. Possible vapC16, toxin, part of toxin-antitoxin (TA) operon with Rv2231B (See Pandey and Gerdes, 2005). Nucleotide position 2505919 in the genome sequence has been corrected, A:G resulting in A81A.; Possible toxin VapC16 2506161 vapC16 14515883 vapC16 Mycobacterium tuberculosis H37Rv Possible toxin VapC16 YP_007410917.1 2505736 R 83332 CDS YP_007410918.1 448817045 14515901 complement(2506207..2506383) 1 NC_000962.3 Rv2231B, len: 58 aa. Possible vapB16, antitoxin,part of toxin-antitoxin (TA) operon with Rv2231A (See Pandey and Gerdes, 2005).; Possible antitoxin VapB16 2506383 vapB16 14515901 vapB16 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB16 YP_007410918.1 2506207 R 83332 CDS NP_216748.2 57116958 887597 2506278..2507153 1 NC_000962.3 Rv2232, (MTCY427.13), len: 291 aa. PtkA, protein tyrosine kinase, similar to members of haloacid dehalogenase-like family from several bacteria and to putative phosphatases e.g. Q9I767 and AAK78398. Contains N-terminal extension. FASTA scores: Q9I767 hypothetical protein PA0065 (221 aa) opt: 439 E(): 3.2e-18; 38.679% identity (40.196% ungapped) in 212 aa overlap; >>tr|AAK78398 Predicted phosphatase, had family (216 aa) opt: 427, E(): 1.5e-17; 34.762% identity (35.437% ungapped) in 210 aa overlap. Replaces previous Rv2232 and Rv2233. Predicted to be an outer membrane protein (See Song et al.,2008).; Protein tyrosine kinase transcriptional regulatory protein PtkA 2507153 ptkA 887597 ptkA Mycobacterium tuberculosis H37Rv Protein tyrosine kinase transcriptional regulatory protein PtkA NP_216748.2 2506278 D 83332 CDS NP_216750.1 15609371 887373 2507146..2507637 1 NC_000962.3 Rv2234, (MTCY427.15), len: 163 aa. PtpA (alternate gene name: MPtpA), low molecular weight protein-tyrosine-phosphatase (see citations below), similar to other phosphotyrosine protein phosphatases e.g. P53433|PTPA_STRCO low molecular weight protein-tyrosine phosphatase from Streptomyces coelicolor (164 aa), FASTA scores: opt: 455, E(): 3.3e -25, (49.7% identity in 155 aa overlap); PA1S_HUMAN|P24667 red cell acid phosphatase 1,FASTA score: (37.7% identity in 138 aa overlap); etc. Contains a phosphatase catalytic site domain located in N-terminal part. Activity proven biochemically. Supposed a secreted protein. Substrate of PtkA|Rv2232.; Phosphotyrosine protein phosphatase PtpA (protein-tyrosine-phosphatase) (PTPase) (LMW phosphatase) 2507637 ptpA 887373 ptpA Mycobacterium tuberculosis H37Rv Phosphotyrosine protein phosphatase PtpA (protein-tyrosine-phosphatase) (PTPase) (LMW phosphatase) NP_216750.1 2507146 D 83332 CDS NP_216751.1 15609372 887606 2507637..2508452 1 NC_000962.3 Rv2235, (MTCY427.16), len: 271 aa. Probable conserved transmembrane protein (see Miller & Shinnick 2001); hydrophobic regions near N- and C-terminus. Similar to conserved membrane proteins in other Actinomycetes. Equivalent to Mycobacterium leprae. ML1644 (270 aa). FASTA scores: opt: 1357, E(): 1.2e-72; 74.170% identity in 271 aa overlap T44717|3150235|CAA19213.1|AL023635 13093419|CAC30595.1|AL583922. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved transmembrane protein 2508452 887606 Rv2235 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216751.1 2507637 D 83332 CDS NP_216752.1 15609373 887513 complement(2508434..2509375) 1 NC_000962.3 Rv2236c, (MTCY427.17c), len: 313 aa. Probable cobD,cobalamin biosynthesis transmembrane protein, similar to S52223 Rhodobacter capsulatus 945 protein BluD (39.0% identity in 287 aa overlap) involved in cobinamide synthesis, and to COBD_PSEDE Pseudomonas dentrificans cobD protein (37.5% identity in 269 aa overlap), also CBIB_SALTY Salmonella typhimurum cbiB protein (35.5% identity in 304 aa overlap).; Probable cobalamin biosynthesis transmembrane protein CobD 2509375 cobD 887513 cobD Mycobacterium tuberculosis H37Rv Probable cobalamin biosynthesis transmembrane protein CobD NP_216752.1 2508434 R 83332 CDS NP_216753.1 15609374 887840 2509489..2510256 1 NC_000962.3 Rv2237, (MTCY427.18), len: 255 aa. Conserved protein. Similar to Mycobacterium tuberculosis hypothetical proteins Rv0276, Rv0826, Rv1645c. FASTA score: Rv0276 gp|AL021930|MTV035_4 (306 aa) opt: 874, E(): 0; 49.6% identity in 282 aa overlap; hypothetical protein 2510256 887840 Rv2237 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216753.1 2509489 D 83332 CDS YP_004837056.2 448824779 14515864 complement(2510351..2510587) 1 NC_000962.3 Rv2237A, len: 78 aa. Conserved protein.; hypothetical protein 2510587 14515864 Rv2237A Mycobacterium tuberculosis H37Rv hypothetical protein YP_004837056.2 2510351 R 83332 CDS NP_216754.1 15609375 887871 complement(2510715..2511176) 1 NC_000962.3 Rv2238c, (MTCY427.19c), len: 153 aa. Probable ahpE,peroxiredoxin. Similarity to many members of AHPC/TSA family e.g. sp|Q96291|BAS1_ARATH 2-CYS peroxiredoxin BAS1 precursor (265 aa). FASTA score: opt: 275, E(): 2.7e-12; 35.0% identity in 143 aa overlap.; Probable peroxiredoxin AhpE 2511176 ahpE 887871 ahpE Mycobacterium tuberculosis H37Rv Probable peroxiredoxin AhpE NP_216754.1 2510715 R 83332 CDS NP_216755.1 15609376 888451 complement(2511176..2511652) 1 NC_000962.3 Rv2239c, (MTCY427.20c), len: 158 aa. Conserved hypothetical protein, similar to conserved hypothetical proteins from Mycobacterium leprae (ML1649, 140 aa) and Streptomyces coelicolor A3(2) (SCC8A.28c, 159 aa). Equivalent to ML1649 conserved hypothetical protein (140 aa). FASTA scores: ML1649 conserved hypothetical protein (140 aa) opt: 846, E(): 6.5e-45; 86.429% identity in 140 aa overlap (tr|O69479|O69479 hypothetical 15.2 KDA protein (140 aa); and opt: 447, E(): 1.2e-21; 50.355% identity (51.825% ungapped) in 141 aa overlap. Similarity with ML1649 suggests alternative start at 251198.; hypothetical protein 2511652 888451 Rv2239c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216755.1 2511176 R 83332 CDS NP_216756.2 448824780 888600 complement(2511690..2512280) 1 NC_000962.3 Rv2240c, (MTCY427.21c), len: 196 aa. Unknown protein. Start changed since first submission (-69 aa).; hypothetical protein 2512280 888600 Rv2240c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216756.2 2511690 R 83332 CDS NP_216757.3 448824781 887246 2512539..2515244 1 NC_000962.3 Rv2241, (MTCY427.22), len: 901 aa. AceE, pyruvate dehydrogenase E1 component, similar to others e.g. ODP1_ECOLI|P06958 pyruvate dehydrogenase E1 component from Escherichia coli, FASTA score: (51.2% identity in 891 aa overlap); etc.; Pyruvate dehydrogenase E1 component AceE (pyruvate decarboxylase) (pyruvate dehydrogenase) (pyruvic dehydrogenase) 2515244 aceE 887246 aceE Mycobacterium tuberculosis H37Rv Pyruvate dehydrogenase E1 component AceE (pyruvate decarboxylase) (pyruvate dehydrogenase) (pyruvic dehydrogenase) NP_216757.3 2512539 D 83332 CDS NP_216758.1 15609379 888624 2515304..2516548 1 NC_000962.3 Rv2242, (MTCY427.23), len: 414 aa. Conserved hypothetical protein. Equivalent to ML1652 conserved hypothetical protein from Mycobacterium leprae (414 aa),and orthologue in Streptomyces coelicolor A3(2). FASTA scores: ML1652 opt: 2369, E(): 4.2e-128; 88.406% identity in 414 aa overlap (AL023635)(AL583922). Some similarity at 3' end with S25203 srmR protein - Streptomyces ambofaciens (604 aa) opt: 188 E(): 9e-05; (26.4% identity in 277 aa overlap) and with SW:YAEG_HAEIN P44509 hypothetical protein HI0093 (42.3% identity in 52 aa overlap). Contains possible helix-turn-helix motif at aa 360-381 (+3.52 SD); hypothetical protein 2516548 888624 Rv2242 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216758.1 2515304 D 83332 CDS NP_216759.1 15609380 888769 2516787..2517695 1 NC_000962.3 Rv2243, (MTCY427.24), len: 302 aa. FabD (alternate gene name: mtFabD), malonyl CoA-acyl carrier protein transacylase (see citations below), highly similar to e.g. A57356 acyl-CoA carrier protein malonyltransferase from Streptomyces coelicolor (316 aa), FASTA score: opt: 955,E(): 0, (52.6% identity in 304 aa overlap); FABD_HAEIN|P43712 malonyl CoA-acyl carrier protein transacylase from Haemophilus influenzae, FASTA score: (30.5% identity in 308 aa overlap); and FABD_ECOLI|P25715 from Escherichia coli, FASTA score: (31.4% identity in 309 aa overlap). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006).; Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT) 2517695 fabD 888769 fabD Mycobacterium tuberculosis H37Rv Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT) NP_216759.1 2516787 D 83332 CDS NP_216760.1 15609381 888272 2517771..2518118 1 NC_000962.3 Rv2244, (MT2304, MTCY427.25), len: 115 aa. AcpM,acyl carrier protein, meromycolate precursor transport,involved in meromycolate extension (see citations below). Highly similar to others e.g. L43074|STMFABD2|STMFABD|g870805 acyl carrier protein from Streptomyces glaucescens (82 aa), FASTA scores: opt: 298,E(): 8.4e-13, (56.6% identity in 76 aa overlap); and ACP_ECOLI|P02901 acyl carrier protein from Escherichia coli, FASTA score: (37.3% identity in 67 aa overlap); etc.; Meromycolate extension acyl carrier protein AcpM 2518118 acpM 888272 acpM Mycobacterium tuberculosis H37Rv Meromycolate extension acyl carrier protein AcpM NP_216760.1 2517771 D 83332 CDS NP_216761.1 15609382 887269 2518115..2519365 1 NC_000962.3 Rv2245, (MTCY427.26), len: 416 aa. KasA,beta-ketoacyl-ACP synthase, involved in meromycolate extension (see citations below): belongs to the fas-II system, which utilizes primarily palmitoyl-ACP rather than short-chain acyl-ACP primers. Highly similar to others e.g. L43074|STMFABD3|g870805 beta-ketoacyl-ACP synthase from Streptomyces glaucescens (423 aa), FASTA scores: opt: 1105,E(): 0, (44.6% identity in 417 aa overlap); FABF_ECOLI|P39435 3-oxoacyl-[acyl-carrier-protein] synthase II from Escherichia coli, FASTA score: (39.4% identity in 254 aa overlap); FABB_HORVU|P23902 3-oxoacyl-[acyl-carrier-protein] synthase I, FASTA score: (33.4% identity in 413 aa overlap); etc. Strongest similarity to downstream ORF kasB|Rv2246|MTCY427.27 3-oxoacyl-[acyl-carrier-protein] synthase 2 from Mycobacterium tuberculosis (438 aa), FASTA score: (66.3% identity in 409 aa overlap). Belongs to the beta-ketoacyl-ACP synthases family.; 3-oxoacyl-[acyl-carrier protein] synthase 1 KasA (beta-ketoacyl-ACP synthase) (KAS I) 2519365 kasA 887269 kasA Mycobacterium tuberculosis H37Rv 3-oxoacyl-[acyl-carrier protein] synthase 1 KasA (beta-ketoacyl-ACP synthase) (KAS I) NP_216761.1 2518115 D 83332 CDS NP_216762.1 15609383 887539 2519396..2520712 1 NC_000962.3 Rv2246, (MTCY427.27), len: 438 aa. KasB,beta-ketoacyl-ACP synthase, involved in meromycolate extension (see citations below). Highly similar or similar to others e.g. L43074|STMFABD3|g870805 beta-ketoacyl-ACP synthase from Streptomyces glaucescens (423 aa), FASTA scores: opt: 1091, E(): 0, (44.7% identity in 416 aa overlap); FABF_ECOLI|P39435 3-oxoacyl-[acyl-carrier-protein] synthase II from Escherichia coli, FASTA score: (37.0% identity in 411 aa overlap); FABB_HORVU|P23902 3-oxoacyl-[acyl-carrier-protein] synthase I, FASTA score: (32.5% identity in 415 aa overlap); etc. Strongest similarity to upstream ORF Rv2245|kasA|MTCY427.26 3-oxoacyl-[acyl-carrier-protein] synthase 1 from Mycobacterium tuberculosis (416 aa), FASTA score: (66.3% identity in 409 aa overlap). Belongs to the beta-ketoacyl-ACP synthases family.; 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I) 2520712 kasB 887539 kasB Mycobacterium tuberculosis H37Rv 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I) NP_216762.1 2519396 D 83332 CDS NP_216763.1 15609384 887671 2520743..2522164 1 NC_000962.3 Rv2247, (MTCY427.28), len: 473 aa. AccD6,Acetyl/Propionyl CoA Carboxylase, beta subunit (see citations below), highly similar to e.g. PCCB_RHOSO|Q06101 propionyl-CoA carboxylase beta chain, FASTA score: (75.1% identity in 437 aa overlap). Similar to many other Acetyl/Propionyl CoA Carboxylases from Mycobacterium tuberculosis. Belongs to the AccD / PccB family.; Acetyl/propionyl-CoA carboxylase (beta subunit) AccD6 2522164 accD6 887671 accD6 Mycobacterium tuberculosis H37Rv Acetyl/propionyl-CoA carboxylase (beta subunit) AccD6 NP_216763.1 2520743 D 83332 CDS NP_216764.1 15609385 888603 2522360..2523175 1 NC_000962.3 Rv2248, (MTCY427.29), len: 271 aa. Conserved hypothetical protein. Very similar to hypothetical M. tuberculosis proteins Rv3517, Rv1482c, Rv3555c, Rv3714c,Rv1073. FASTA score: MTCY06G11.02c MTCY6G11 NID: g1877284 -(289 aa) opt: 366 E(): 5.3e-18; (32.1% identity in 249 aa overlap). Some similarity to Mycobacterium avium protein AF002133|AF0021 339 AF002133 NID: g2183254 (346 aa) opt: 308 E(): 5.2e-14; (28.3% identity in 254 aa overlap); hypothetical protein 2523175 888603 Rv2248 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216764.1 2522360 D 83332 CDS NP_216765.1 15609386 887276 complement(2523241..2524791) 1 NC_000962.3 Rv2249c, (MTCY427.31c), len: 516 aa. Probable glpD1,glycerol-3-phosphate dehydrogenase, similar to SW:GLPD_ECOLI P13035 aerobic glycerol-3-phosphate dehydrogenase (30.0% identity in 486 aa overlap) and SW:GLPA_ECOLI P13032 anaerobic glycerol-3-phosphate dehydrogenase (28.2% identity in 504 aa overlap). Also similar to Rv3302c|glpD2 glycerol-3-phosphate dehydrogenase. Cofactor: FAD (by similarity). Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.; Probable glycerol-3-phosphate dehydrogenase GlpD1 2524791 glpD1 887276 glpD1 Mycobacterium tuberculosis H37Rv Probable glycerol-3-phosphate dehydrogenase GlpD1 NP_216765.1 2523241 R 83332 CDS NP_216766.2 57116959 888018 complement(2524785..2525354) 1 NC_000962.3 Rv2250c, (MTCY427.32c), len: 189 aa. Possible transcriptional regulatory protein, TetR family. Start unclear; ORF has been shortened since first submission to avoid overlap with Rv2251 (-30 aa). Contains probable helix-turn-helix motif (Score 2243, +6.70 SD); Possible transcriptional regulatory protein 2525354 888018 Rv2250c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_216766.2 2524785 R 83332 CDS YP_177662.1 57116960 3205100 2525402..2525821 1 NC_000962.3 Rv2250A, len: 139 aa. Conserved hypothetical protein, possibly flavoprotein. Similar to N-terminus of SCF91.28c|AL132973_28 possible flavoprotein from Streptomyces coelicolor (530 aa), FASTA scores: opt: 240,E(): 1.1e-07, (39.25% identity in 107 aa overlap). Possible frameshift between nt 2525723 to 2525727. The sequences of CDC 1551 and Mycobacterium bovis are missing a single G base.; Possible flavoprotein 2525821 3205100 Rv2250A Mycobacterium tuberculosis H37Rv Possible flavoprotein YP_177662.1 2525402 D 83332 CDS NP_216767.1 15609388 888706 2525565..2526992 1 NC_000962.3 Rv2251, (MTV022.01), len: 475 aa. Possible flavoprotein, probably continuation of Rv2250A, similar to MTCY164.18 from Mycobacterium tuberculosis and to several alkyldihydroxyacetonephosphate synthases (e.g. O00116). Also some similarity to D-lactate dehydrogenases. FASTA scores: sptr|O05784|O05784 hypothetical 56.5 kDa protein. (527 aa) opt: 1019 E(): 0; (38.6% identity in 487 aa overlap) and sp|O00116|ADAS_HUMAN alkyldihydroxyaceton ephosphate synthase precursor (658 aa) opt: 558 E(): 6.2e-27; (31.3% identity in 447 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible flavoprotein 2526992 888706 Rv2251 Mycobacterium tuberculosis H37Rv Possible flavoprotein NP_216767.1 2525565 D 83332 CDS NP_216768.1 15609389 888429 2526989..2527918 1 NC_000962.3 Rv2252, (MTV022.02), len: 309 aa. Diacylglycerol kinase (See Owens et al., 2006), similar to hypothetical proteins from Bacillus subtilis (e.g. BSUB0004_120),Streptomyces coelicolor A3(2) >emb|CAB61184.1| (AL132973) hypothetical protein SCF91.27c (293 aa) and P39074. FASTA scores: Z99107|BSUB0004_120 Bacillus subtilis complete genome (303 aa) opt: 397, E(): 1.7e-19; (26.4% identity in 299 aa overlap) and P390 74|BMRU_BACSU BMRU protein (297 aa) opt: 309, E(): 1.3e-13; (25.0% identity in 284 aa overlap).; Diacylglycerol kinase 2527918 888429 Rv2252 Mycobacterium tuberculosis H37Rv Diacylglycerol kinase NP_216768.1 2526989 D 83332 CDS NP_216769.1 15609390 888287 2527984..2528487 1 NC_000962.3 Rv2253, (MTV022.03), len: 167 aa. Possible secreted protein; has potential N-terminal signal peptide. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible secreted unknown protein 2528487 888287 Rv2253 Mycobacterium tuberculosis H37Rv Possible secreted unknown protein NP_216769.1 2527984 D 83332 CDS NP_216770.1 15609391 887182 complement(2528520..2528975) 1 NC_000962.3 Rv2254c, (MTV022.04c), len: 151 aa. Probable integral membrane protein.; Probable integral membrane protein 2528975 887182 Rv2254c Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_216770.1 2528520 R 83332 CDS NP_216771.1 15609392 885526 complement(2528980..2529174) 1 NC_000962.3 Rv2255c, (MTV022.05c), len: 64 aa. Hypothetical unknown protein.; Hypothetical protein 2529174 885526 Rv2255c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216771.1 2528980 R 83332 CDS NP_216772.1 15609393 885865 complement(2529341..2529874) 1 NC_000962.3 Rv2256c, (MTV022.06c), len: 177 aa. Conserved hypothetical protein, similar to Streptomyces glaucescens ORF5 (164 aa) and Streptomyces coelicolor hypothetical protein SC4A7.19c (164 aa; emb|CAB62723.1|AL133423). FASTA scores: sptr|Q54209|Q54209 FABD, FABH, FABC, FABB, and ORF5 (164 aa) opt: 504, E(): 3.9e-27; (44.4% identity in 162 aa overlap).; hypothetical protein 2529874 885865 Rv2256c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216772.1 2529341 R 83332 CDS NP_216773.1 15609394 888069 complement(2530004..2530822) 1 NC_000962.3 Rv2257c, (MTV022.07c), len: 272 aa. Conserved protein, similar to hypothetical protein SC4A7.08 from Streptomyces coelicolor (273 aa; 58% identity in 243 aa overlap). Also similar to several putative esterases and penicillin-binding proteins in M. tuberculosis e.g. Rv1923,Rv1497, Rv2463, Rv3775, Rv1922, Rv1730c.; hypothetical protein 2530822 888069 Rv2257c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216773.1 2530004 R 83332 CDS NP_216774.1 15609395 888755 complement(2530836..2531897) 1 NC_000962.3 Rv2258c, (MTV022.08c), len: 353 aa. Possible transcriptional regulatory protein, similar to several hypothetical proteins from C. elegans. FASTA scores: sptr|O01593|O01593 coded for by C. elegans CDNA YK102 F (365 aa) opt: 577, E(): 6.4e-31; (30.5% identity in 341 aa overlap). Contains possible helix-turn helix motif at aa 47-68 (+3.65 SD); Possible transcriptional regulatory protein 2531897 888755 Rv2258c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_216774.1 2530836 R 83332 CDS NP_216775.1 15609396 887215 2532245..2533330 1 NC_000962.3 Rv2259, (MTV022.09), len: 361 aa. MscR,S-nitrosomycothiol reductase (see Vogt et al., 2003),similar to several zinc-containing alcohol dehydrogenases especially from Amycolatopsis methanolica P80094 (360 aa),FASTA scores: sp|P80094|FADH_AMYME NAD/mycothiol-dependent formaldehyde dehydrogenase (MD-FALDH) Length = 360, Expect = e-156, Identities = 268/358 (74%). Also similar to Rv0162c, (MTCI28.02c, 35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Note previously known as adhE2; S-nitrosomycothiol reductase MscR 2533330 mscR 887215 mscR Mycobacterium tuberculosis H37Rv S-nitrosomycothiol reductase MscR NP_216775.1 2532245 D 83332 CDS NP_216776.1 15609397 888490 2533330..2533965 1 NC_000962.3 Rv2260, (MTV022.10), len: 211 aa. Conserved hypothetical protein, similar to hypothetical proteins Rv0634c, Rv1637c, Rv3677c, Rv2581c from Mycobacterium tuberculosis and to various hydrolases. FASTA scores: sptr|O06154|O06154 hypothetical 21.3 kDa protein (200 aa) opt: 355, E(): 4e- 15; (37.4% identity in 198 aa overlap).; hypothetical protein 2533965 888490 Rv2260 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216776.1 2533330 D 83332 CDS NP_216777.1 15609398 887455 complement(2534042..2534464) 1 NC_000962.3 Rv2261c, (MTV022.11c), len: 140 aa. Conserved hypothetical protein, with function unknown but some similarity to C-terminal end of PCC6803 apolipoprotein N-acyltransferase from Synechocystis sp. Note that next ORF shows similarity to N-terminal part of P74055 apolipoprotein N-acyltransferase from Escherichia coli (519 aa), FASTA scores: opt: 142, E(): 0.007, (29.9% identity in 117 aa overlap), suggesting possible frameshift. Sequence of clones from two sources has been checked but no error found.; hypothetical protein 2534464 887455 Rv2261c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216777.1 2534042 R 83332 CDS NP_216778.1 15609399 887220 complement(2534470..2535552) 1 NC_000962.3 Rv2262c, (MTV022.12c), len: 360 aa. Conserved hypothetical protein, with function unknown but some similarity to N-terminal 70% of P23930|P77703|LNT_ECOLI|cute|B0657 apolipoprotein N-acyltransferase from Escherichia coli strain K12 (512 aa), FASTA scores: opt: 239, E(): 1.6e-07, (30.4% identity in 359 aa overlap). Note that neighboring ORF shows similarity to N -terminal part of PCC6803 apolipoprotein N-acyltransferase from Synechocystis sp., suggesting possibility of frameshift. Sequence of clones from two sources has been checked but no error found. Appear to be two extra bases at position 1876970 compared to CDC1551 strain.; hypothetical protein 2535552 887220 Rv2262c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216778.1 2534470 R 83332 CDS NP_216779.1 15609400 887788 2535641..2536594 1 NC_000962.3 Rv2263, (MTV022.13), len: 317 aa. Possible oxidoreductase, similar to several oxidoreductases. Similarity suggests alternative GTG start at 10154 but then no rbs. FASTA scores: sptr|Q544 05|Q54405 probably an NADP-dependent oxidoreductase (297 aa) opt: 487, E(): 1.1e-23; (36.1% identity in 299 aa overlap). Also similar to Mycobacterium tuberculosis Rv0068, and Rv0439c.; Possible oxidoreductase 2536594 887788 Rv2263 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_216779.1 2535641 D 83332 CDS NP_216780.1 15609401 888040 complement(2536572..2538350) 1 NC_000962.3 Rv2264c, (MTV022.14c), len: 592 aa. Conserved hypothetical Pro-rich protein, similar to hypothetical proteins Rv0312 (MTCY63.17, 620 aa and Rv0350) that has highly Pro-, Thr-rich C-terminus. Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. FASTA scores: Z96800|MTCY63_17 Mycobacterium tuberculosis cosmid (620 aa) opt: 1075, E(): 8.8e-24; (38.9% identity in 627 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Conserved hypothetical proline rich protein 2538350 888040 Rv2264c Mycobacterium tuberculosis H37Rv Conserved hypothetical proline rich protein NP_216780.1 2536572 R 83332 CDS NP_216781.1 15609402 887976 2538700..2539929 1 NC_000962.3 Rv2265, (MTCY339.45c), len: 409 aa. Possible conserved integral membrane protein, with some similarity to others e.g. M. thermoauto. sp|O26855|O26855 conserved protein (383 aa), FASTA score: opt: 898 z-score: 1023.5 E(): 0; 38.0% identity in 384 aa overlap; Q58713 hypothetical 44.1 kDa protein 1 317 (398 aa), FASTA scores,opt: 305 E(): 1.2e-11; 22.8% identity in 382 aa overlap; also KGTP_ECOLI P17448 alpha-ketoglutarate permease (432 aa), FASTA scores, opt: 156, E(): 0.006, (24.8% identity in 416 aa overlap); Possible conserved integral membrane protein 2539929 887976 Rv2265 Mycobacterium tuberculosis H37Rv Possible conserved integral membrane protein NP_216781.1 2538700 D 83332 CDS NP_216782.1 15609403 887763 2540104..2541390 1 NC_000962.3 Rv2266, (MT2328, MTCY339.44c), len: 428 aa. Probable cyp124, cytochrome P450, similar to e.g. G405543 cytochrome P450 (406 aa), FASTA scores, opt: 763,E(): 0, (35.4% identity in 393 aa overlap), similar to e.g. MTCY50.26,33.8% identity in 370 aa overlap; Probable cytochrome P450 124 Cyp124 2541390 cyp124 887763 cyp124 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 124 Cyp124 NP_216782.1 2540104 D 83332 CDS NP_216783.1 15609404 888487 complement(2541644..2542810) 1 NC_000962.3 Rv2267c, (MTCY339.43), len: 388 aa. Conserved hypothetical protein; some similarity to Mycobacterium tuberculosis Rv3529c; gp|Z82098|MTCY3C7_27 (384 aa) FASTA score: opt: 261, E(): 3.6e-10; 27.3% identity in 253 aa overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 2542810 888487 Rv2267c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216783.1 2541644 R 83332 CDS NP_216784.1 15609405 888025 complement(2542807..2544276) 1 NC_000962.3 Rv2268c, (MT2330, MTCY339.42), len: 489 aa. Probable cyp128, cytochrome P450, similar to (but longer than) cytochrome p-450 e.g. CPXK_SACER P3 3271 cytochrome p-450 107b1 (405 aa), FASTA scores, opt: 620, E(): 8.3e-33,(31.8% identity in 406 aa overlap); contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature,similar to MTCY50.26, 32.7% identity in 382 aa overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable cytochrome P450 128 Cyp128 2544276 cyp128 888025 cyp128 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 128 Cyp128 NP_216784.1 2542807 R 83332 CDS NP_216785.1 15609406 887623 complement(2544289..2544621) 1 NC_000962.3 Rv2269c, (MTCY339.41), len: 110 aa. Unknown protein; questionable ORF. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2544621 887623 Rv2269c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216785.1 2544289 R 83332 CDS NP_216786.1 15609407 887448 2544698..2545225 1 NC_000962.3 Rv2270, (MTCY339.40c), len: 175 aa. Probable lppN,lipoprotein; has appropriately positioned prokaryotic membrane lipoprotein attachment site PS00013.; Probable lipoprotein LppN 2545225 lppN 887448 lppN Mycobacterium tuberculosis H37Rv Probable lipoprotein LppN NP_216786.1 2544698 D 83332 CDS NP_216787.1 15609408 887223 2545332..2545631 1 NC_000962.3 Rv2271, (MTCY339.39c), len: 99 aa. Conserved hypothetical protein; some similarity to hypothetical protein AAK01340.1|AF265275_3 (AF265275) from uncultured organism Pu8 (104 aa) E= 4e-10, (34% identity in 91 aa overlap); hypothetical protein 2545631 887223 Rv2271 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216787.1 2545332 D 83332 CDS NP_216788.1 15609409 887459 2545737..2546105 1 NC_000962.3 Rv2272, (MTCY339.38c), len: 122 aa. Probable conserved transmembrane protein, similar to YIDH_ECOLI P31445 hypothetical 12.8 kDa protein (115 aa), FASTA scores, opt: 291, E(): 2.9e-14, (45.6% identity in 103 aa overlap), similar to MTCY339.37c, (35.0% identity in 100 aa overlap).; Probable conserved transmembrane protein 2546105 887459 Rv2272 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216788.1 2545737 D 83332 CDS NP_216789.1 15609410 888440 2546102..2546431 1 NC_000962.3 Rv2273, (MTCY339.37c), len: 109 aa. Probable conserved transmembrane protein, similar to Rv2272 (MTCY339.38c), (35.0% identity in 100 aa overlap).; Probable conserved transmembrane protein 2546431 888440 Rv2273 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216789.1 2546102 D 83332 CDS NP_216790.1 15609411 888067 complement(2546488..2546805) 1 NC_000962.3 Rv2274c, (MTCY339.36), len: 105 aa. Possible mazF8,toxin, part of toxin-antitoxin (TA) operon with Rv2274A (See Pandey and Gerdes, 2005). Questionable ORF.; Possible toxin MazF8 2546805 mazF8 888067 mazF8 Mycobacterium tuberculosis H37Rv Possible toxin MazF8 NP_216790.1 2546488 R 83332 CDS YP_007410963.1 448817090 14515891 complement(2546839..2547087) 1 NC_000962.3 Rv2274A, len: 82 aa. Possible mazE8, antitoxin, part of toxin-antitoxin (TA) operon with Rv2274c (See Pandey and Gerdes, 2005).; Possible antitoxin MazE8 2547087 mazE8 14515891 mazE8 Mycobacterium tuberculosis H37Rv Possible antitoxin MazE8 YP_007410963.1 2546839 R 83332 CDS NP_216791.1 15609412 888355 2546883..2547752 1 NC_000962.3 Rv2275, (MTCY339.35c), len: 289 aa. Conserved hypothetical protein. Some similarity to Bacillus subtilis sp|O34351|O34351 YVMC (248 aa), FASTA score: opt: 280, E(): 2.7e -11; 28.2% identity in 227 aa overlap; hypothetical protein 2547752 888355 Rv2275 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216791.1 2546883 D 83332 CDS NP_216792.1 15609413 888373 2547749..2548939 1 NC_000962.3 Rv2276, (MT2336, MTCY339.34c), len: 396 aa. Cyp121,cytochrome P450 (see citation below), similar to e.g. G303644 (397 aa) opt: 675, z-score: 776.4, E(): 2.7e-36,(33.7% identity in 407 aa overlap); contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature,similar to MTCY339.42, 29.2% identity in 298 aa overlap.; Cytochrome P450 121 Cyp121 2548939 cyp121 888373 cyp121 Mycobacterium tuberculosis H37Rv Cytochrome P450 121 Cyp121 NP_216792.1 2547749 D 83332 CDS NP_216793.1 15609414 888498 complement(2549124..2550029) 1 NC_000962.3 Rv2277c, (MTCY339.33), len: 301 aa. Possible glycerolphosphodiesterase, similar to e.g. UGPQ_ECOLI P10908 glycerophosphoryldiester phosphodiesterase (cytosolic) (247 aa), FASTA scores, opt: 149, E(): 0.0061,(27.2% identity in 195 aa overlap). Start of protein uncertain, encoded by neighbouring IS6110 as given, is intact in Mycobacterium tuberculosis CDC1551; Possible glycerolphosphodiesterase 2550029 888498 Rv2277c Mycobacterium tuberculosis H37Rv Possible glycerolphosphodiesterase NP_216793.1 2549124 R 83332 CDS NP_216794.1 15609415 888602 2550065..2550391 1 NC_000962.3 Rv2278, (MTCY339.32c), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2278 and Rv2279,the sequence UUUUAAAG (directly upstream of Rv2279) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Putative transposase for insertion sequence element IS6110 (fragment) 2550391 888602 Rv2278 Mycobacterium tuberculosis H37Rv Putative transposase for insertion sequence element IS6110 (fragment) NP_216794.1 2550065 D 83332 CDS NP_216795.2 448824782 887746 <2550340..2551326 1 NC_000962.3 Rv2279, (MTCY339.31c), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2278 and Rv2279, the sequence UUUUAAAG (directly upstream of Rv2279) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa).; Probable transposase 2551326 887746 Rv2279 Mycobacterium tuberculosis H37Rv Probable transposase NP_216795.2 <2550340 D 83332 CDS NP_216796.1 15609417 887601 2551560..2552939 1 NC_000962.3 Rv2280, (MTCY339.30c), len: 459 aa. Probable dehydrogenase. Similar to D-lactate dehydrogenase (cytochrome) precursor e.g. G1061264 (587 aa), FASTA scores, opt: 645,E(): 1.3e-31, (28.0% identity in 478 aa overlap), similar to MTCY50.25, 36.5% identity in 447 aa overlap; Probable dehydrogenase 2552939 887601 Rv2280 Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_216796.1 2551560 D 83332 CDS NP_216797.1 15609418 887257 2553173..2554831 1 NC_000962.3 Rv2281, (MTCY339.29c), len: 552 aa. Putative pitB,phosphate-transport permease, integral membrane protein,similar to YG04_HAEIN P45268 putative phosphate permease hi1604 (420 aa). FASTA scores, opt: 484, E(): 5e-23, (33.5% identity in 498 aa overlap) also to G399598 amphotropic murine retrovirus receptor (656 aa) FASTA scores, opt: 453,E(): 5.8e-21, (26.8% identity in 645 aa overlap). Also similar to Rv0545c|pitA from M. tuberculosis. Belongs to the pit subfamily.; Putative phosphate-transport permease PitB 2554831 pitB 887257 pitB Mycobacterium tuberculosis H37Rv Putative phosphate-transport permease PitB NP_216797.1 2553173 D 83332 CDS NP_216798.1 15609419 887253 complement(2554938..2555876) 1 NC_000962.3 Rv2282c, (MTCY339.28), len: 312 aa. Probable transcriptional regulator, lysR family, similar to others e.g. YC30_CYAPA|P48271 hypothetical transcriptional regulator YCF30 (324 aa), FASTA scores: opt: 292, E(): 4e-12, (27.6% identity in 286 aa overlap); etc. Also similar to Rv0377|MTCY39.34 from Mycobacterium tuberculosis, FASTA score: (25.4% identity in 268 aa overlap). Contains PS00044 Bacterial regulatory proteins,lysR family signature, and contains helix-turn-helix motif at aa 24 -45 (+4.93 SD).; Probable transcription regulator (LysR family) 2555876 887253 Rv2282c Mycobacterium tuberculosis H37Rv Probable transcription regulator (LysR family) NP_216798.1 2554938 R 83332 CDS NP_216799.1 15609420 887644 2555941..2556135 1 NC_000962.3 Rv2283, (MTCY339.27c), len: 64 aa. Unknown protein; questionable ORF.; Hypothetical protein 2556135 887644 Rv2283 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216799.1 2555941 D 83332 CDS NP_216800.1 15609421 887794 2556145..2557440 1 NC_000962.3 Rv2284, (MTCY339.26c), len: 431 aa. Probable lipM,esterase, similar to others e.g. gp|Z95844|MTCY493_28 from Mycobacterium tuberculosis cosmid (420 aa), FASTA scores: opt: 1266, E(): 0, (50.1% identity in 411 aa overlap). Some similarity to G537514 arylacetamide deacetylase (399 aa),FASTA scores: opt: 190, E(): 5.9e-05, (30.4% identity in 138 aa overlap).; Probable esterase LipM 2557440 lipM 887794 lipM Mycobacterium tuberculosis H37Rv Probable esterase LipM NP_216800.1 2556145 D 83332 CDS NP_216801.1 15609422 888632 2557473..2558810 1 NC_000962.3 Rv2285, (MTCY339.25c), len: 445 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), member of Mycobacterium tuberculosis 15-membered protein family including Rv3740c, Rv3734c, Rv1425, Rv1760, Rv0895,Rv3480c. FASTA scores: gp|Z95844|MTCY493_29 Mycobacterium tuberculosis cosmid (459 aa) opt: 640, E(): 0; 33.4% identity in 470 aa overlap.; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 2558810 888632 Rv2285 Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_216801.1 2557473 D 83332 CDS NP_216802.1 15609423 887395 complement(2558877..2559569) 1 NC_000962.3 Rv2286c, (MTCY339.24), len: 230 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis hypothetical protein, Rv2466c, AL021246|MTV008_22 (207 aa). FASTA score: opt: 324, E(): 8.9e-15; 30.4% identity in 194 aa overlap; hypothetical protein 2559569 887395 Rv2286c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216802.1 2558877 R 83332 CDS NP_216803.1 15609424 887570 2559703..2561331 1 NC_000962.3 Rv2287, (MTCY339.23c), len: 542 aa. Probable yjcE,conserved integral membrane transport protein, similar to eukaryote NA+/H+ exchangers e.g. YJCE_ECOLI|P32703|B4065 Putative Na(+)/H(+) exchanger from Escherichia coli (549 aa), FASTA scores: opt: 436, E(): 5.6e-21, (29.4% identity in 555 aa overlap); etc. Seems to belong to CPA1 family (NA(+)/H(+) exchanger family).; Probable conserved integral membrane transport protein YjcE 2561331 yjcE 887570 yjcE Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein YjcE NP_216803.1 2559703 D 83332 CDS NP_216804.1 15609425 887702 2561328..2561705 1 NC_000962.3 Rv2288, (MTCY339.22c), len: 125 aa. Unknown hypothetical protein; Hypothetical protein 2561705 887702 Rv2288 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216804.1 2561328 D 83332 CDS NP_216805.1 15609426 887342 2561675..2562457 1 NC_000962.3 Rv2289, (MTCY339.21c), len: 260 aa. Probable cdh,CDP-diacylglycerol pyrophosphatase, similar to CDH_SALTY|P26219 cdp-diacylglycerol pyrophosphatase (251 aa), FASTA scores: opt: 395, E(): 5.9e-20, (33.5% identity in 221 aa overlap).; Probable CDP-diacylglycerol pyrophosphatase Cdh (CDP-diacylglycerol diphosphatase) (CDP-diacylglycerol phosphatidylhydrolase) 2562457 cdh 887342 cdh Mycobacterium tuberculosis H37Rv Probable CDP-diacylglycerol pyrophosphatase Cdh (CDP-diacylglycerol diphosphatase) (CDP-diacylglycerol phosphatidylhydrolase) NP_216805.1 2561675 D 83332 CDS NP_216806.1 15609427 887203 2562599..2563114 1 NC_000962.3 Rv2290, (MTCY339.20c), len: 171 aa. Probable lppO,conserved lipoprotein, similar to Rv3763, 19KD_MYCTU P11572 19 kDa lipoprotein antigen precursor (159 aa) FASTA scores,opt: 119, E (): 1.3, (25.6% identity in 164 aa overlap). Contains appropriately positioned PS00013 lipoprotein motif (with one mismatch). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable conserved lipoprotein LppO 2563114 lppO 887203 lppO Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppO NP_216806.1 2562599 D 83332 CDS NP_216807.1 15609428 887174 2563174..2564028 1 NC_000962.3 Rv2291, (MTCY339.19c), len: 284 aa. Probable sseB,thiosulfate sulfurtransferase. Very similar to thiosulfate sulfurtransferas/rhodanese from Streptomyces coelicolor AL00920 4|SC9B10_21 (283 aa) opt: 765, E(): 0; Smith-Waterman score: 765; 46.9% identity in 286 aa overlap, similar to THTR_ECOLI P31142 putative thiosulfate sulfurtransferase (280 aa), FASTA scores, opt: 478, E(): 1e-23, (35.1% identity in 265 aa overlap); Probable thiosulfate sulfurtransferase SseB 2564028 sseB 887174 sseB Mycobacterium tuberculosis H37Rv Probable thiosulfate sulfurtransferase SseB NP_216807.1 2563174 D 83332 CDS NP_216808.1 15609429 888805 complement(2564029..2564253) 1 NC_000962.3 Rv2292c, (MTCY339.18), len: 74 aa. Unknown hypothetical protein; Hypothetical protein 2564253 888805 Rv2292c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216808.1 2564029 R 83332 CDS NP_216809.1 15609430 887283 complement(2564292..2565032) 1 NC_000962.3 Rv2293c, (MTCY339.17), len: 246 aa. Conserved hypothetical protein; some similarity to hypothetical protein (299 aa) AAK24237.1| (AE005897) belonging to phosphorylase family [Caulobacter crescentus] (33% identity in 131 aa overlap). Possible lipoprotein: signal peptide at N-terminus; hypothetical protein 2565032 887283 Rv2293c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216809.1 2564292 R 83332 CDS NP_216810.1 15609431 885868 2565327..2566550 1 NC_000962.3 Rv2294, (MTCY339.16c), len: 407 aa. Probable aminotransferase, similar to others in M. tuberculosis e.g. MTV030_19, also similar to PATB_BACSU|Q08432 putative aminotransferase b from Bacillus subtilis (387 aa), FASTA scores: opt: 563, E(): 2.8e-29, (31.4% identity in 408 aa overlap); and to MALY_ECOLI|P23256 maly protein from Escherichia coli (390 aa), FASTA scores: opt: 530, E(): 3.6e-27, (31.3% identity in 384 aa overlap). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases.; Probable aminotransferase 2566550 885868 Rv2294 Mycobacterium tuberculosis H37Rv Probable aminotransferase NP_216810.1 2565327 D 83332 CDS NP_216811.1 15609432 888540 2566772..2567410 1 NC_000962.3 Rv2295, (MTCY339.15c), len: 212 aa. Conserved hypothetical protein, cysteine-rich protein, similar to YIEJ_ECOLI P31469 hypothetical 22.5 kDa protein in tnab-bglb intergenic region (195 aa), opt: 270, E(): 3.4e-11, (36.4% identity in 198 aa overlap). Alternative start suggested by similarity 26 codons further downstream; hypothetical protein 2567410 888540 Rv2295 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216811.1 2566772 D 83332 CDS NP_216812.1 15609433 887796 2567504..2568406 1 NC_000962.3 Rv2296, (MTCY339.14c), len: 300 aa. Probable haloalkane dehalogenase, similar to e.g. HALO_XANAU P22643,haloalkane dehalogenase, (310 aa), opt: 510 z-score: 577.7 E(): 3.1e-25 (39.0% identity in 315 aa overlap).; Probable haloalkane dehalogenase 2568406 887796 Rv2296 Mycobacterium tuberculosis H37Rv Probable haloalkane dehalogenase NP_216812.1 2567504 D 83332 CDS NP_216813.1 15609434 887789 2568438..2568890 1 NC_000962.3 Rv2297, (MTCY339.13c), len: 150 aa. Unknown protein; contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide; hypothetical protein 2568890 887789 Rv2297 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216813.1 2568438 D 83332 CDS NP_216814.1 15609435 887344 2569082..2570053 1 NC_000962.3 Rv2298, (MTCY339.12c), len: 323 aa. Conserved protein. Similar to SLR0545 Synechocystis sp, Q55493 hypothetical 34.6 kDa protein (314 aa), FASTA scores, opt: 427, E(): 1.7e-20, (39.3% identity in 303 aa overlap) and to YZAE_BACSU P46905 hypothetical protein in natb 3'region (268 aa) FASTA scores, opt: 370, E(): 6.1e-17, (31.4% identity in 264 aa overlap); hypothetical protein 2570053 887344 Rv2298 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216814.1 2569082 D 83332 CDS NP_216815.1 15609436 887501 complement(2570059..2572002) 1 NC_000962.3 Rv2299c, (MTCY339.11), len: 647 aa. HtpG, probable chaperone, heat shock protein 90 family. Similar to HTPG_BACSU|P46208 heat shock protein htpG homologue from Bacillus subtilis (626 aa), FASTA scores: opt: 1551, E(): 0, (39.6% identity in 631 aa overlap). Contains possible helix-turn-helix motif at aa 519-540 (+3.77 SD).; Probable chaperone protein HtpG (heat shock protein) (HSP90 family protein) (high temperature protein G) 2572002 htpG 887501 htpG Mycobacterium tuberculosis H37Rv Probable chaperone protein HtpG (heat shock protein) (HSP90 family protein) (high temperature protein G) NP_216815.1 2570059 R 83332 CDS NP_216816.1 15609437 887880 complement(2572076..2573008) 1 NC_000962.3 Rv2300c, (MTCY339.09), len: 310 aa (start uncertain). Conserved protein, similar to others e.g. Q9RXY2|DR0172 conserved hypothetical protein from Deinococcus radiodurans (271 aa), FASTA scores: opt: 306,E(): 1.3e-12, (34.6% identity in 229 aa overlap); Q9HZH1|PA3037 hypothetical protein from Pseudomonas aeruginosa (288 aa), FASTA scores: opt: 248, E(): 7.9e-09,(31.5% identity in 238 aa overlap); Q9PDL8|XF1361 hypothetical protein from Xylella fastidiosa (279 aa),FASTA scores: opt: 236, E(): 4.6e-08, (29.7% identity in 249 aa overlap); U70053|XCU70053_3 GumP protein from Xanthomonas campestris (282 aa), FASTA scores: opt: 222,E(): 3.7e-07, (30.1% identity in 248 aa overlap); etc.; hypothetical protein 2573008 887880 Rv2300c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216816.1 2572076 R 83332 CDS NP_216817.2 57116961 885371 2573015..2573707 1 NC_000962.3 Rv2301, (MTCY339.08c), len: 230 aa. Probable cut2 (alternate gene name: cfp25), cutinase, highly similar to others from Mycobacteria tuberculosis e.g. MTCY13E12.04|Rv3451|O06318|CUT3_MYCTU (247 aa), FASTA scores: opt: 569, E(): 2.3e-27, (45.3% identity in 223 aa overlap); MT2037|MTCY39.35|RV1984C|Q10837|CUT1_MYCTU (217 aa), FASTA scores: opt: 383, E(): 3.4e-16 (42.9% identity in 217 aa overlap); O69691|Rv3724|MTV025.072 putative cutinase precursor (187 aa), FASTA scores: opt: 248, E(): 4.3e-08, (41.85% identity in 172 aa overlap); etc. Also similar to few others from other organisms e.g. Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa),FASTA scores: opt: 391, E(): 1.1e-16, (39.15% identity in 235 aa overlap); etc. Contains PS00095 C-5 cytosine-specific DNA methylases C-terminal signature. Belongs to the cutinase family. Start changed since first submission (+11 aa).; Probable cutinase Cut2 2573707 cut2 885371 cut2 Mycobacterium tuberculosis H37Rv Probable cutinase Cut2 NP_216817.2 2573015 D 83332 CDS NP_216818.1 15609439 885154 2573813..2574055 1 NC_000962.3 Rv2302, (MTCY339.07c), len: 80 aa. Conserved protein, highly similar to others: O53766|AL021942|Rv0569|MTV039.07 hypothetical 9.5 KDA protein from Mycobacterium tuberculosis (88 aa), FASTA scores: opt: 300, E(): 1.4e-14, (61.85% identity in 76 aa overlap); O88049|SCI35.11 hypothetical 7.1 KDA protein from Streptomyces coelicolor (64 aa), FASTA scores: opt: 169,E(): 1.5e-05, (46.55% identity in 58 aa overlap) (has its C-terminus shorter); Q9XCD1 hypothetical 12.0 KDA protein (fragment) from Thermomonospora fusca (106 aa), FASTA scores: opt: 126, E(): 0.023, (50.0% identity in 34 aa overlap) (similarity in part for this one). Also weakly similar to U650M|G699303|Q50105 hypothetical 5.7 KDA protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 89, E(): 0.66, (45.5% identity in 33 aa overlap); and weakly similar to N-terminus of Q9RIZ1|SCJ1.23c putative DNA-binding protein from Streptomyces coelicolor (323 aa),FASTA scores: opt: 182, E(): 7.3e-06, (42.25% identity in 71 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 2574055 885154 Rv2302 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216818.1 2573813 D 83332 CDS NP_216819.1 15609440 885282 complement(2574096..2575019) 1 NC_000962.3 Rv2303c, (MTCY339.06, MT2360), len: 307 aa. Probable antibiotic-resistance protein, with some similarity to Q54229|G153373 macrotetrolide antibiotic-resistance protein (NONR) from Streptomyces griseus (347 aa) (see Plater and Robinson, 1992), FASTA scores: opt: 438, E(): 3.1e-21,(33.2% identity in 226 aa overlap); and other hypothetical proteins e.g. P95886 ORF C02006 from Sulfolobus solfataricus (269 aa), FASTA scores: opt: 252, E(): 3.5e-09, (25.5% identity in 286 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv3510c|O53555|MTV023.17. Note that the protein Q9XDF3|NONC from Streptomyces griseus subsp. griseus (317 aa) is equivalent to Q54229|G153373|NONR however the N-terminal end is shorter (30 aa) owing to a changed start codon (see Walczak et al., 2000). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable antibiotic-resistance protein 2575019 885282 Rv2303c Mycobacterium tuberculosis H37Rv Probable antibiotic-resistance protein NP_216819.1 2574096 R 83332 CDS NP_216820.1 15609441 885102 complement(2575016..2575225) 1 NC_000962.3 Rv2304c, (MTCY339.05), len: 69 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2575225 885102 Rv2304c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216820.1 2575016 R 83332 CDS NP_216821.1 15609442 885752 2575809..2577098 1 NC_000962.3 Rv2305, (MTCY339.04c), len: 429 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 2577098 885752 Rv2305 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216821.1 2575809 D 83332 CDS YP_177663.1 57116962 3205062 2577108..2577701 1 NC_000962.3 Rv2306A, len: 197 aa. Possible conserved membrane protein, similar to several hypothetical membrane proteins from Mycobacterium tuberculosis and Streptomyces coelicolor, e.g. Rv0625c|P96915|Y625_MYCTU hypothetical 25.2 KDA protein from Mycobacterium tuberculosis (246 aa),FASTA scores: opt: 410, E(): 2.7e-17, (53.25% identity in 139 aa overlap). First 140 aa show high similarity, this then decreases but continues in next ORF Rv2306B,suggesting a frameshift near nt 2577473. However the sequence has been checked and no error found. The sequence is identical in CDC1551 and Mycobacterium bovis. Replaces original Rv2306c on other strand. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible conserved membrane protein 2577701 3205062 Rv2306A Mycobacterium tuberculosis H37Rv Possible conserved membrane protein YP_177663.1 2577108 D 83332 CDS YP_177664.1 57116963 3205063 2577488..2577922 1 NC_000962.3 Rv2306B, len: 144 aa. Possible conserved membrane protein, similar to C-terminal part of several hypothetical membrane proteins from Mycobacterium tuberculosis and Streptomyces coelicolor e.g. P96915|Y625_MYCTU|RV0625c hypothetical 25.2 KDA protein from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 480, E(): 5e-24,(77.15% identity in 92 aa overlap). Could be a continuation of Rv2306A suggesting there may be a frameshift near nt 2577473. The C-terminal part is longer than Rv0625c and the 3'-end of gene overlaps Rv2307c, so maybe a further framehift. However, sequence has been checked and no error found. Also same sequence as strain CDC1551 and Mycobacterium bovis. Replaces original Rv2306c on other strand. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible conserved membrane protein 2577922 3205063 Rv2306B Mycobacterium tuberculosis H37Rv Possible conserved membrane protein YP_177664.1 2577488 D 83332 CDS NP_216823.1 15609444 885277 complement(2577851..2578696) 1 NC_000962.3 Rv2307c, (MTCY339.02), len: 281 aa. Conserved hypothetical protein, similar to many other hypothetical proteins and BEM1/BUD5 suppressors e.g. P77538 hypothetical protein from Escherichia coli (293 aa), FASTA scores: opt: 421, E(): 2.4e-18, (32.1% identity in 268 aa overlap) (alias AAG57647|Z3802|BAB36823|ECS3400 Putative enzyme (3.4.-) from Escherichia coli (293 aa), FASTA scores: opt: 425, E(): 1.7e-18, (32.1% identity in 268 aa overlap));P54069|BE46_SCHPO|BEM46|SPBC32H8.03|PI020 BEM46 protein from Schizosaccharomyces pombe (Fission yeast) (352 aa), FASTA scores: opt: 355, E(): 3.3e-14, (30.45% identity in 279 aa overlap); O76462|BEM46 BEM46 protein from Drosophila melanogaster (338 aa), FASTA scores: opt: 404,E(): 2.8e-17, (32.75% identity in 281 aa overlap); etc. Equivalent (but with few differences) to AAK46650|MT2364 protein from Mycobacterium tuberculosis strain CDC1551 (281 aa). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 2578696 885277 Rv2307c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216823.1 2577851 R 83332 CDS YP_177665.1 57116964 3205073 complement(2579228..2579419) 1 NC_000962.3 Rv2307A, len: 63 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical glycine rich protein 2579419 3205073 Rv2307A Mycobacterium tuberculosis H37Rv Hypothetical glycine rich protein YP_177665.1 2579228 R 83332 CDS YP_177666.1 57116965 3205074 complement(2579504..2579935) 1 NC_000962.3 Rv2307B, len: 143 aa. Hypothetical unknown Gly- rich protein. Equivalent to AAK46653 from Mycobacterium tuberculosis strain CDC1551 (133 aa) but longer 10 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical glycine rich protein 2579935 3205074 Rv2307B Mycobacterium tuberculosis H37Rv Hypothetical glycine rich protein YP_177666.1 2579504 R 83332 CDS YP_177667.1 57116966 3205075 complement(2580028..2580210) 1 NC_000962.3 Rv2307D, len: 60 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 2580210 3205075 Rv2307D Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177667.1 2580028 R 83332 CDS NP_216824.1 15609445 885290 2580419..2581135 1 NC_000962.3 Rv2308, (MTCY339.01c), len: 238 aa. Conserved hypothetical protein, sharing similarity with O53464|Rv2018|MTV018.05 from Mycobacterium tuberculosis (239 aa), FASTA scores: opt: 142, E(): 0.034, (24.8% identity in 250 aa overlap). As contains possible helix-turn-helix motif at aa 16-37 (Sequence: YVYAEVDKLIGLPAGTAKRWIN) (Score 1169, +3.17 SD), may be a transcriptional regulator. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 2581135 885290 Rv2308 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216824.1 2580419 D 83332 CDS NP_216825.1 15609446 885133 complement(2581843..2582298) 1 NC_000962.3 Rv2309c, (MTCY3G12.25), len: 151 aa. Possible integrase (fragment), similar to others e.g. Q48908 integrase (fragment) from Mycobacterium paratuberculos (191 aa), FASTA scores: opt: 279, E(): 3.2e-11, (40.4% identity in 136 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv1055|MTV017.08 integrase (fragment) (78 aa) (72.85% identity in 70 aa overlap); and Rv1054|MTV017.07 integrase (fragment). Could belong to the 'phage' integrase family. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible integrase (fragment) 2582298 885133 Rv2309c Mycobacterium tuberculosis H37Rv Possible integrase (fragment) NP_216825.1 2581843 R 83332 CDS YP_177668.1 57116967 3205076 2583045..2583332 1 NC_000962.3 Rv2309A, len: 95 aa. Hypothetical unknown protein. Equivalent to AAK46663 from Mycobacterium tuberculosis strain CDC1551 (95 aa) but longer 13 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 2583332 3205076 Rv2309A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177668.1 2583045 D 83332 CDS NP_216826.1 15609447 885175 2583435..2583779 1 NC_000962.3 Rv2310, (MT2372, MTCY3G12.24c), len: 114 aa. Possible excisionase, showing some similarity to others e.g. Q9LCU5 putative excisionase from Arthrobacter sp. TM1 (174 aa) FASTA scores: opt: 341, E(): 6.6e-15, (48.2% identity in 110 aa overlap); O85865 putative excisionase from Sphingomonas aromaticivorans (152 aa), FASTA scores: opt: 205, E(): 2.2e-06, (41.25% identity in 80 aa overlap); etc. Also similar to Rv3750c|O69717 hypothetical protein from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 228, E(): 6.9e-08, (43.9% identity in 82 aa overlap). Contains possible helix-turn-helix motif at aa 20-41 (Score 2181, +6.62 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible excisionase 2583779 885175 Rv2310 Mycobacterium tuberculosis H37Rv Possible excisionase NP_216826.1 2583435 D 83332 CDS NP_216827.1 15609448 885168 2583884..2584408 1 NC_000962.3 Rv2311, (MTCY3G12.23c), len: 174 aa. Conserved hypothetical protein, with similarity (in part) to transfer proteins homologous TRAA e.g. Q9EUN8|TRAA transfer protein homolog TRAA from Corynebacterium glutamicum (1160 aa),FASTA scores: opt: 221, E(): 2.9e-07, (36.8% identity in 136 aa overlap); Q9ETQ3|TRAA conjugal transfer protein (TRAA-like protein) from Corynebacterium equii (1367 aa),FASTA scores: opt: 188, E(): 5.5e-05, (33% identity in 106 aa overlap); P55418|TRAA_RHISN|Y4DS probable conjugal transfer protein from Rhizobium sp. strain NGR234 (1102 aa), FASTA scores: opt: 145, E(): 0.035, (29.08% identity in 141 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 2584408 885168 Rv2311 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216827.1 2583884 D 83332 CDS NP_216828.1 15609449 885152 2584486..2584755 1 NC_000962.3 Rv2312, (MTCY3G12.22c), len: 89 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2584755 885152 Rv2312 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216828.1 2584486 D 83332 CDS NP_216829.1 15609450 885268 complement(2585052..2585906) 1 NC_000962.3 Rv2313c, (MTCY3G12.21), len: 284 aa. Hypothetical unknown protein.; Hypothetical protein 2585906 885268 Rv2313c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216829.1 2585052 R 83332 CDS NP_216830.1 15609451 885130 complement(2585917..2587290) 1 NC_000962.3 Rv2314c, (MTCY3G12.20), len: 457 aa. Conserved protein, highly similar to Q9RJ51|SCI8.02 hypothetical protein from Streptomyces coelicolor (464 aa) FASTA scores: opt: 1485, E(): 5.2e-83, (53.5% identity in 454 aa overlap); similar to AAK24788|CC2824 TldD/PmbA family protein from Caulobacter crescentus (441 aa), FASTA scores: opt: 364, E(): 8.3e-15, (29.8% identity in 460 aa overlap); and showing similarity with Q9HJZ6|TA0814 hypothetical protein from Thermoplasma acidophilum (430 aa), FASTA scores: opt: 220, E(): 4.7e-06, (21.85% identity in 348 aa overlap).; hypothetical protein 2587290 885130 Rv2314c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216830.1 2585917 R 83332 CDS NP_216831.1 15609452 885260 complement(2587287..2588804) 1 NC_000962.3 Rv2315c, (MTCY3G12.19), len: 505 aa. Conserved protein, highly similar to Q9S273|SCI28.10 hypothetical 47.1 KDA protein from Streptomyces coelicolor (435 aa),FASTA scores: opt: 1768, E():5.6e-101, (63.2% identity in 432 overlap); and similar to others e.g. AAK24787|CC2823 hypothetical protein (TldD/PmbA family) from Caulobacter crescentus (543 aa), FASTA scores: opt: 876, E():3.1e-46,(42.8% identity in 505 overlap); O58578|PH0848 hypothetical 54.4 KDA protein from Pyrococcus horikoshii (481 aa), FASTA scores: opt: 661, E(): 4.3e-33, (29.95% identity in 484 aa overlap); Q9UZ95|PAB1547 hypothetical 53.6 KDA protein from Pyrococcus abyssi (473 aa), FASTA scores: opt: 656, E(): 8.6e-33, (29.1% identity in 481 aa overlap); etc.; hypothetical protein 2588804 885260 Rv2315c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216831.1 2587287 R 83332 CDS NP_216832.1 15609453 885262 2588838..2589710 1 NC_000962.3 Rv2316, (MTCY3G12.18c), len: 290 aa. Probable uspA,sugar-transport integral membrane protein ABC transporter (see citation below), most similar to Q9CBN8|USPA|ML1768 sugar transport integral membrane protein from Mycobacterium leprae (328 aa), FASTA scores: opt: 1593,E(): 1.9e-93, (82.35% identity in 289 aa overlap); and similar to O32940|ML1426|MLCB2052.28 possible sugar transport protein (probable ABC-transport protein, inner membrane component) from Mycobacterium leprae (319 aa),FASTA scores: opt: 600, E(): 9.2e-31, (34.25% identity in 295 aa overlap). Also similar to other proteins involved in transport e.g. Q9X860|SCE134.05c putative binding protein dependent transport protein from Streptomyces coelicolor (327 aa), FASTA scores: opt: 639, E(): 3.2e-33, (40.45% identity in 272 aa overlap); Q9K6N9|BH3689 sugar transport system (permease) from Bacillus halodurans (300 aa), FASTA scores: opt: 590, E(): 3.7e-30, (35.65% identity in 289 aa overlap); etc.; Probable sugar-transport integral membrane protein ABC transporter UspA 2589710 uspA 885262 uspA Mycobacterium tuberculosis H37Rv Probable sugar-transport integral membrane protein ABC transporter UspA NP_216832.1 2588838 D 83332 CDS YP_177866.1 57116968 885101 2589697..2590521 1 NC_000962.3 Rv2317, (MTC3G12.17c), len: 274 aa. Probable uspB,sugar-transport integral membrane protein ABC transporter (see citation below), most similar to Q9CBN7|USPE|ML1769 sugar transport integral membrane protein from Mycobacterium leprae (274 aa), FASTA scores: opt: 1522,E(): 3.4e-89, (85.0% identity in 274 aa overlap); and similar to O32941|ML1425|MLCB2052.29 probable ABC-transport protein, inner membrane component from Mycobacterium leprae (283 aa), FASTA scores: opt: 630, E(): 8.4e-33, (36.55% identity in 268 aa overlap). Also similar to other integral membrane proteins e.g. P73854|LACG|SLR1723 lactose transport system permease protein from Synechocystis sp. strain PCC 6803 (270 aa), FASTA scores: opt: 605, E(): 3.1e-31, (36.0% identity in 264 aa overlap); Q9F3B8|SC5F1.11 putative sugar transport integral membrane protein from Streptomyces coelicolor (307 aa), FASTA scores: opt: 582, E(): 9.7e-30, (34.45% identity in 264 aa overlap); etc. Also similar to O53483|Rv2039c|MTV018.26c sugar transport protein from Mycobacterium tuberculosis (280 aa), FASTA scores: opt: 630, E(): 8.3e-89, (37.7% identity in 268 aa overlap).; Probable sugar-transport integral membrane protein ABC transporter UspB 2590521 uspB 885101 uspB Mycobacterium tuberculosis H37Rv Probable sugar-transport integral membrane protein ABC transporter UspB YP_177866.1 2589697 D 83332 CDS NP_216834.1 15609455 885143 2590518..2591840 1 NC_000962.3 Rv2318, (MTCY3G12.16c), len: 440 aa. Probable uspC,sugar-binding lipoprotein component of sugar transport system (see citation below), most similar to Q9CBN6|USPC|ML1770 sugar transport periplasmic binding protein from Mycobacterium leprae (446 aa), FASTA scores: opt: 2294, E(): 8.1e-135, (74.7% identity in 446 aa overlap). Also similar to other substrate-binding proteins e.g. Q9RK89|SCF1.15 putative substrate binding protein (extracellular) (binding-protein-dependent transport) (fragment) from Streptomyces coelicolor (221 aa), FASTA scores: opt: 377, E(): 3e-16, (32.25% identity in 217 aa overlap); Q9K6N8|BH3690 sugar transport system (sugar-binding protein) from Bacillus halodurans (420 aa),FASTA scores: opt: 227, E(): 1e-06, (25.00% identity in 452 aa overlap); etc. Also similar to O53485|Rv2041c|MTV018.28C lipoprotein component of sugar transport system from Mycobacterium tuberculosis (439 aa), FASTA scores: opt: 246, E(): 7e-08, (26.75% identity in 325 aa overlap). Contains a hydrophobic stretch (possible signal peptide) at N-terminal end.; Probable periplasmic sugar-binding lipoprotein UspC 2591840 uspC 885143 uspC Mycobacterium tuberculosis H37Rv Probable periplasmic sugar-binding lipoprotein UspC NP_216834.1 2590518 D 83332 CDS NP_216835.1 15609456 885171 complement(2591848..2592726) 1 NC_000962.3 Rv2319c, (MTCY3G12.15), len: 292 aa. Universal stress protein family protein.; Universal stress protein family protein 2592726 885171 Rv2319c Mycobacterium tuberculosis H37Rv Universal stress protein family protein NP_216835.1 2591848 R 83332 CDS NP_216836.1 15609457 885084 complement(2592723..2594153) 1 NC_000962.3 Rv2320c, (MTCY3G12.14), len: 476 aa. Probable rocE,cationic amino acid (especially arginine and ornithine) transporter (permease), highly similar to other amino acid transporters e.g. Q9L100|SCL6.16C putative amino acid transporter from Streptomyces coelicolor (496 aa), FASTA scores: opt: 1485, E(): 9.4e-82, (48.4% identity in 477 aa overlap); O06479|YFNA putative amino acid transporter from Bacillus subtilis (462 aa), FASTA scores: opt: 1271, E(): 6.1e-69, (41.9% identity in 463 aa overlap); Q9PG94|XF0408 amino acid transporter from Xylella fastidiosa (509 aa),FASTA scores: opt: 1128, E(): 2.5e-60, (39.5% identity in 481 aa overlap); etc. Also some similarity with Z99108.1|BSUB0005 from Bacillus subtilis (461 aa), FASTA scores: opt: 1271, E(): 0, (41.9% identity in 463 aa overlap); and G403170 ethanolamine permease (488 aa), FASTA scores: opt: 468, E(): 1e-23, (28.1% identity in 462 aa overlap). Seems to belong to the APC family.; Probable cationic amino acid transport integral membrane protein RocE 2594153 rocE 885084 rocE Mycobacterium tuberculosis H37Rv Probable cationic amino acid transport integral membrane protein RocE NP_216836.1 2592723 R 83332 CDS NP_216837.1 15609458 885056 complement(2594154..2594699) 1 NC_000962.3 Rv2321c, (MTCY3G12.13), len: 181 aa. Probable rocD2,ornithine aminotransferase, highly similar to C-terminal region of other ornithine aminotransferases, e.g. Q9FC90|ROCD from Streptomyces coelicolor (407 aa), FASTA scores: opt: 628, E(): 1.2e-32, (55.35% identity in 168 aa overlap); P3802|OAT_BACSU|ROCD from Bacillus subtilis (401 aa), FASTA scores: opt: 477, E(): 4.3e-23, (42.1% identity in 178 aa overlap); BAB42057|ROCD|SA0818 from Staphylococcus aureus subsp. aureus N315 (396 aa), FASTA scores: opt: 437, E(): 1.5e-20, (41.3% identity in 170 aa overlap); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Rv2322c|MTCY3G12.12 (upstream ORF) and Rv2321c|MTCY3G12.13 appear to be an ornithine aminotransferase homologue but are frameshifted - we can find no sequence error in the cosmid to account for this.; Probable ornithine aminotransferase (C-terminus part) RocD2 (ornithine--oxo-acid aminotransferase) 2594699 rocD2 885056 rocD2 Mycobacterium tuberculosis H37Rv Probable ornithine aminotransferase (C-terminus part) RocD2 (ornithine--oxo-acid aminotransferase) NP_216837.1 2594154 R 83332 CDS NP_216838.1 15609459 885096 complement(2594699..2595364) 1 NC_000962.3 Rv2322c, (MTCY3G12.12), len: 221 aa. Probable rocD1,ornithine aminotransferase, highly similar to N-terminal region of other ornithine aminotransferases, e.g. Q9FC90|ROCD from Streptomyces coelicolor (407 aa), FASTA scores: opt: 770, E(): 8.7e-40, (55.7% identity in 201 aa overlap); BAB42057|ROCD|SA0818 from Staphylococcus aureus subsp. aureus N315 (396 aa) FASTA scores: opt: 632, E(): 2.2e-31, (46.1% identity in 208 aa overlap); P38021|OAT_BACSU|ROCD from Bacillus subtilis (401 aa),FASTA scores: opt: 626, E(): 5.1e-31, (43.1% identity in 218 aa overlap); etc. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Rv2322c|MTCY3G12.12 and Rv2321c|MTCY3G12.13 (upstream ORF) appear to be an ornithine aminotransferase homologue but are frameshifted - we can find no sequence error in the cosmid to account for this.; Probable ornithine aminotransferase (N-terminus part) RocD1 (ornithine--oxo-acid aminotransferase) 2595364 rocD1 885096 rocD1 Mycobacterium tuberculosis H37Rv Probable ornithine aminotransferase (N-terminus part) RocD1 (ornithine--oxo-acid aminotransferase) NP_216838.1 2594699 R 83332 CDS NP_216839.1 15609460 885480 complement(2595361..2596269) 1 NC_000962.3 Rv2323c, (MTCY3G12.11), len: 302 aa. Conserved protein, highly similar to others e.g. Q9FC91|2SCG58.22 conserved hypothetical protein from Streptomyces coelicolor (288 aa), FASTA scores: opt: 561, E(): 7.3e-28, (46.95% identity in 279 aa overlap); P74535|SLL1336 hypothetical 78.3 KDA protein from Synechocystis sp. (705 aa), FASTA scores: opt: 555, E(): 2.1e-27, (37.75% identity in 265 aa overlap); etc. Also similar to various hydrolases e.g. Q53797 beta-hydroxylase (bleomycin/phleomycin binding protein, ankyrin homologue, bleomycin and transport protein) from Streptomyces verticillus (326 aa), FASTA scores: opt: 211, E(): 4.5e-06, (26.75% identity in 303 aa overlap); Q9X7M4|DDAH_STRCO|SC5F2A.01c NG,NG-dimethylarginine dimethylaminohydrolase (Dimethylargininase) (Dimethylarginine dimethylaminohydrolase) (258 aa), FASTA scores: opt: 209,E(): 4.9e-06, (27.15% identity in 243 aa overlap); G434715 beta-hydroxylase (bleomicin/phleomycin binding protein) from Streptomyces verticillus (326 aa), FASTA scores: opt: 211, E(): 4.5e-06, (26.75% identity in 303 aa overlap); etc.; hypothetical protein 2596269 885480 Rv2323c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216839.1 2595361 R 83332 CDS NP_216840.1 15609461 885060 2596334..2596780 1 NC_000962.3 Rv2324, (MTCY3G12.10), len: 148 aa. Probable transcriptional regulatory protein, asnC-family, similar to other putative AsnC-family regulatory proteins e.g. Q9L101|SCL6.15C from Streptomyces coelicolor (150 aa) FASTA scores: opt: 466, E(): 2.4e-24, (52.8% identity in 142 aa overlap); Q9RKY4|SC6D7.14 putative AsnC-family transcriptional regulatory protein from Streptomyces coelicolor (165 aa), FASTA scores: opt: 266, E(): 5.5e-11,(32.4% identity in 145 aa overlap); Q9ZEP1|LRPA|SCE94.12c putative transcriptional regulator from Streptomyces coelicolor (150 aa), FASTA scores: opt: 249, E(): 6.9e-10,(33.35% identity in 147 aa overlap); etc. Also similar to P96896|Rv3291c|MTCY71.31c from Mycobacterium tuberculosis (150 aa), FASTA scores: opt: 261, E(): 1.1e-10, (36.4% identity in 143 aa overlap).; Probable transcriptional regulatory protein (probably AsnC-family) 2596780 885060 Rv2324 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably AsnC-family) NP_216840.1 2596334 D 83332 CDS NP_216841.1 15609462 886271 complement(2597009..2597857) 1 NC_000962.3 Rv2325c, (MTCY3G12.09), len: 282 aa. Conserved hypothetical protein, equivalent to O32970|MLCB22.37c|ML0849 hypothetical protein from Mycobacterium leprae (283 aa), FASTA scores: opt: 1405,E(): 1.8e-78, (77.7% identity in 282 aa overlap). Also some similarity to other proteins e.g. Q9Z9J1|YBAF|BH0166 YBAF protein (BH0166 protein) (hypothetical protein) from Bacillus halodurans (265 aa), FASTA scores: opt: 288, E(): 2.8e-10, (25.8% identity in 264 aa overlap); P70972|YBAF YBAF protein (hypothetical protein) from Bacillus subtilis (265 aa), FASTA scores: opt: 259, E(): 1.5e-08, (25.45% identity in 224 aa overlap); AAK34821|SPY2193|Q99X13 Conserved hypothetical protein from Streptococcus pyogenes (266 aa), FASTA scores: opt: 232, E(): 6.5e-07, (25.1% identity in 267 aa overlap); etc.; hypothetical protein 2597857 886271 Rv2325c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216841.1 2597009 R 83332 CDS NP_216842.1 15609463 888184 complement(2597854..2599947) 1 NC_000962.3 Rv2326c, (MTC3G12.08), len: 697 aa. Possible transmembrane ATP-binding protein ABC transporter (see citation below). Equivalent to Q9CCF9|ML0848 ABC transporter from Mycobacterium leprae (724 aa), FASTA scores: opt: 3482, E(): 2.8e-182, (76.9% identity in 697 aa overlap) and also to O32971|MLCB22.38c ABC-type transporter from Mycobacterium leprae (726 aa), FASTA scores: opt: 3482, E(): 2.8e-182, (76.9% identity in 697 aa overlap). Similar in part to other ABC transporters e.g. Q9WY65|TM0222 from Thermotoga maritima (266 aa), FASTA scores: opt: 407, E(): 4.2e-15, (38.0% identity in 213 aa overlap); etc. Contains 2 X PS00017 ATP/GTP-binding site motif A (P-loop); and 2 x PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Possible transmembrane ATP-binding protein ABC transporter 2599947 888184 Rv2326c Mycobacterium tuberculosis H37Rv Possible transmembrane ATP-binding protein ABC transporter NP_216842.1 2597854 R 83332 CDS NP_216843.1 15609464 888124 2599988..2600479 1 NC_000962.3 Rv2327, (MTCY3G12.07c), len: 163 aa. Conserved protein, similar to Z80775|MTCY21D4.05c|Rv0042c from Mycobacterium tuberculosis (208 aa), FASTA scores: opt: 242, E(): 5e-08, (43.0% identity in 107 aa overlap). Also slight similarity to putative transcriptional regulatory proteins belonging to the MarR-family e.g. Q9CCY2/ML2696 from Mycobacterium leprae (243 aa), FASTA scores: opt: 245,E(): 3.7e-08, (35.35% identity in 150 aa overlap); Q9L135|SC6D11.20 from Streptomyces coelicolor (155 aa),FASTA scores: opt: 242, E(): 3.9e-08, (34.75% identity in 141 aa overlap); etc.; hypothetical protein 2600479 888124 Rv2327 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216843.1 2599988 D 83332 CDS YP_177867.1 57116969 888111 2600731..2601879 1 NC_000962.3 Rv2328, (MTCY3G12.06), len: 382 aa. PE23, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to others e.g. Q9L8K5|MAG24-1 PE-PGRS homolog from Mycobacterium marinum (638 aa), FASTA scores: opt: 495, E(): 6.6e-18, (34.65% identity in 401 aa overlap); etc.; PE family protein PE23 2601879 PE23 888111 PE23 Mycobacterium tuberculosis H37Rv PE family protein PE23 YP_177867.1 2600731 D 83332 CDS NP_216845.1 15609466 888116 complement(2601914..2603461) 1 NC_000962.3 Rv2329c, (MTCY3G12.05), len: 515 aa. Probable narK1,nitrite extrusion protein, possibly member of major facilitator superfamily (MFS). Equivalent to O32974|MLCB22.41c|nark|ML0844 putative nitrite extrusion protein from Mycobacterium leprae (517 aa), FASTA scores: opt: 2224, E(): 1.9e-129, (69.3% identity in 488 aa overlap). Also highly similar to others e.g. P94933 nitrite extrusion protein from Mycobacterium fortuitum (471 aa),FASTA scores: opt: 1969, E(): 8.6e-114, (62.1% identity in 459 aa overlap); P37758|NARU_ECOLI nitrite extrusion protein 2 from Escherichia coli strain K12 (462 aa), FASTA scores: opt: 792, E(): 2.3e-41, (36.95% identity in 476 aa overlap); P10903|NARK_ECOLI nitrite extrusion protein (nitrite facilitator 1) from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 784, E(): 7e-41, (35.3% identity in 468 aa overlap); etc. Also similar to RV0261c|Z86089|MTCY6A4_5 from Mycobacterium tuberculosis (469 aa), FASTA scores: opt: 2000, E(): 1.1e-115, (62.6% identity in 470 aa overlap). Belongs to the nark/NASA family of transporters.; Probable nitrite extrusion protein 1 NarK1 (nitrite facilitator 1) 2603461 narK1 888116 narK1 Mycobacterium tuberculosis H37Rv Probable nitrite extrusion protein 1 NarK1 (nitrite facilitator 1) NP_216845.1 2601914 R 83332 CDS NP_216846.1 15609467 888072 complement(2603695..2604222) 1 NC_000962.3 Rv2330c, (MTCY3G12.04), len: 175 aa. Probable lppP,lipoprotein. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LppP 2604222 lppP 888072 lppP Mycobacterium tuberculosis H37Rv Probable lipoprotein LppP NP_216846.1 2603695 R 83332 CDS NP_216847.1 15609468 888099 2604297..2604683 1 NC_000962.3 Rv2331, (MT2393, MTCY3G12.03c), len: 128 aa. Hypothetical unknown protein; shortened version of MTCY3G12.03c to eliminate overlap with MTCY3G12.04.; Hypothetical protein 2604683 888099 Rv2331 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216847.1 2604297 D 83332 CDS YP_177669.1 57116970 3205048 2604740..2605078 1 NC_000962.3 Rv2331A, len: 112 aa. Hypothetical unknown protein.; Hypothetical protein 2605078 3205048 Rv2331A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177669.1 2604740 D 83332 CDS NP_216848.2 57116971 887962 2605108..2606754 1 NC_000962.3 Rv2332, (MTCY3G12.02c, MTCY98.01, MT2394), len: 548 aa. Probable mez, malate oxidoreductase [NAD] dependent (malic enzyme), highly similar to others e.g. O34389|MALS putative malolactic enzyme [includes: malic enzyme ; L-lactate dehydrogenase] from Bacillus subtilis (566 aa),FASTA scores: opt: 1927, E(): 5.5e-111, (52.9% identity in 539 aa overlap); P45868|MAO2_BACSU|YWKA probable NAD-dependent malic enzyme from Bacillus subtilis (582 aa),FASTA scores: opt: 1849, E(): 3.6e-106, (50.45% identity in 543 aa overlap); Q48796|MLES_OENOE malolactic enzyme from Oenococcus oeni (541 aa), FASTA scores: opt: 1540, E(): 3.6e-87, (44.2% identity in 536 aa overlap); etc. Belongs to the malic enzymes family. N-terminus shortened since first submission (previously 652 aa).; Probable [NAD] dependent malate oxidoreductase Mez (malic enzyme) (NAD-malic enzyme) (malate dehydrogenase (oxaloacetate decarboxylating)) (pyruvic-malic carboxylase) (NAD-me) 2606754 mez 887962 mez Mycobacterium tuberculosis H37Rv Probable [NAD] dependent malate oxidoreductase Mez (malic enzyme) (NAD-malic enzyme) (malate dehydrogenase (oxaloacetate decarboxylating)) (pyruvic-malic carboxylase) (NAD-me) NP_216848.2 2605108 D 83332 CDS NP_216849.1 15609470 887274 complement(2606708..2608321) 1 NC_000962.3 Rv2333c, (MTCY3G12.01), len: 537 aa. stp, integral membrane drug efflux protein (See Ramon-Garcia et al.,2007), member of major facilitator superfamily (MFS),highly similar to many e.g. Q9RL22|C5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 1031, E(): 4e-55, (37.4% identity in 412 aa overlap); Q9L0L9|SCD82.12 putative transmembrane efflux protein from Streptomyces coelicolor (490 aa), FASTA scores: opt: 883, E(): 3.8e-46, (36.35% identity in 407 aa overlap); Q9ZBW5|SC4B5.03c putative integral membrane efflux protein from Streptomyces coelicolor (504 aa), FASTA scores: opt: 899, E(): 4.1e-47,(37.4% identity in 415 aa overlap); P39886|TCMA_STRGA tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 839,E(): 1.9e-43, (32.3% identity in 489 aa overlap); etc. Also highly similar to Rv2459|O53186|MTV008.15 probable conserved integral membrane transport protein from Mycobacterium tuberculosis strain H37Rv (508 aa), FASTA scores: opt: 1385, E(): 1.5e-76, (44.05% identity in 504 aa overlap); and AAK46834|MT2534 drug transporter from Mycobacterium tuberculosis strain CDC1551 (523 aa), FASTA scores: opt: 1385, E(): 1.5e-76, (44.4% identity in 504 aa overlap).; Integral membrane drug efflux protein Stp 2608321 stp 887274 stp Mycobacterium tuberculosis H37Rv Integral membrane drug efflux protein Stp NP_216849.1 2606708 R 83332 CDS YP_177868.1 57116972 886016 2608796..2609728 1 NC_000962.3 Rv2334, (MT2397, MTCY98.03), len: 310 aa. cysK1,cysteine synthase A, equivalent to O32978|CYSK_MYCLE|ML0839|MLCB22.47 cysteine synthase a from Mycobacterium leprae (310 aa), FASTA scores: opt: 1756,E(): 8.6e-96, (85.8% identity in 310 aa overlap). Also highly similar to other cysteine synthases e.g. Q9JQL6|CYSK|NMA0974|NMB0763 putative cysteine synthase from Neisseria meningitidis (serogroup a and B) (310 aa), FASTA scores: opt: 1368, E(): 4.6e-73, (66.45% identity in 310 aa overlap); P73410|CYSK_SYNY3|SLR1842 from Synechocystis sp (312 aa), FASTA scores: opt: 1310, E(): 1.2e-69, (64.65% identity in 311 aa overlap); Q43725|CYSM_ARATH|OASC|ACS1|AT3G59760|F24G16.30 cysteine synthase (mitochondrial precursor) from Arabidopsis thaliana (Mouse-ear cress) (424 aa), FASTA scores: opt: 1253, E(): 3.2e-66, (59.2% identity in 309 aa overlap) (has its N-terminus longer 104 aa); etc. Contains PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. Belongs to the cysteine synthase/cystathionine beta-synthase family. Note that previously known as cysK.; Cysteine synthase a CysK1 (O-acetylserine sulfhydrylase A) (O-acetylserine (thiol)-lyase A) (CSASE A) 2609728 cysK1 886016 cysK1 Mycobacterium tuberculosis H37Rv Cysteine synthase a CysK1 (O-acetylserine sulfhydrylase A) (O-acetylserine (thiol)-lyase A) (CSASE A) YP_177868.1 2608796 D 83332 CDS NP_216851.1 15609472 886012 2609732..2610421 1 NC_000962.3 Rv2335, (MTCY98.04), len: 229 aa. Probable cysE,serine acetyltransferase, equivalent to O32979|CYSE|ML0838 serine acetyltransferase from Mycobacterium leprae (227 aa), FASTA scores: opt: 1152, E(): 9.6e-62, (76.4% identity in 229 aa overlap). Also highly similar, except in C-terminal part, to others e.g. Q9HXI6|CYSE|PA3816 O-acetylserine synthase from Pseudomonas aeruginosa (258 aa), FASTA scores: opt: 737, E(): 6e-37, (61.3% identity in 168 aa overlap); P23145|NIFP_AZOCH probable serine acetyltransferase from Azotobacter chroococcum mcd 1 (269 aa), FASTA scores: opt: 718, E(): 8.4e-36, (55.45% identity in 220 aa overlap); Q06750|CYSE_BACSU serine acetyltransferase from Bacillus subtilis (217 aa), FASTA scores: opt: 640, E(): 3.1e-31, (48.0% identity in 200 aa overlap); etc. Contains PS00101 Bacterial hexapeptide-repeat containing-transferases signature. Belongs to the CYSE/LACA/LPXA/NODL family of acetyltransferases. Composed of multiple repeats of [LIV]-G-X(4).; Probable serine acetyltransferase CysE (sat) 2610421 cysE 886012 cysE Mycobacterium tuberculosis H37Rv Probable serine acetyltransferase CysE (sat) NP_216851.1 2609732 D 83332 CDS NP_216852.1 15609473 888958 2610837..2611805 1 NC_000962.3 Rv2336, (MTCY98.05), len: 322 aa. Hypothetical unknown protein (see Rindi et al., 2001). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 2611805 888958 Rv2336 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216852.1 2610837 D 83332 CDS NP_216853.1 15609474 885123 complement(2611869..2612987) 1 NC_000962.3 Rv2337c, (MTCY98.06c), len: 372 aa. Hypothetical unknown protein, sharing some similarity with Q9RI33|SCJ12.27c hypothetical 37.2 KDA protein from Streptomyces coelicolor (335 aa), blast scores: 134 and 46,(28% and 33% identity, 52% and 44% positive); FASTA scores: opt: 176, E(): 0.00042, (31.95% identity in 355 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 2612987 885123 Rv2337c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216853.1 2611869 R 83332 CDS NP_216854.1 15609475 886018 complement(2613107..2614063) 1 NC_000962.3 Rv2338c, (MTCY98.07c), len: 318 aa. Possible moeW,molybdoptenum biosynthesis protein, showing some similarity to several molybdopterin biosynthesis proteins e.g. O27613|MTH1571 molybdopterin biosynthesis protein MOEB homolog from Methanobacterium thermoautotrophicum (251 aa),FASTA scores: opt: 309, E(): 4.7e-14; (30.7% identity in 254 aa overlap); Q9KPQ5|VC2311 HESA/MOEB/THIF family protein from Vibrio cholerae (273 aa), FASTA scores: opt: 255, E(): 4e-09, (36.25% identity in 149 aa overlap); Q9PD34|XF1545 molybdopterin biosynthesis protein from Xylella fastidiosa (276 aa), FASTA scores: opt: 233,E(): 1e-07, (33.6% identity in 128 aa overlap); etc. Seems to belong to the HESA/MOEB/THIF family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible molybdopterin biosynthesis protein MoeW 2614063 moeW 886018 moeW Mycobacterium tuberculosis H37Rv Possible molybdopterin biosynthesis protein MoeW NP_216854.1 2613107 R 83332 CDS NP_216855.1 15609476 888966 2614693..2617581 1 NC_000962.3 Rv2339, (MTCY98.08), len: 962 aa. Probable mmpL9,conserved transmembrane transport protein (see citation below), with strong similarity to other Mycobacterial proteins e.g. P54881|YV34_MYCLE|MML4_MYCLE hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 3799, E(): 0, (59.3% identity in 937 aa overlap); G699237|U1740AB from Mycobacterium leprae; and MTCY20G9.34; MTCY48.08c; MTCY19G5.06 from Mycobacterium tuberculosis. Belongs to the MmpL family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable conserved transmembrane transport protein MmpL9 2617581 mmpL9 888966 mmpL9 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein MmpL9 NP_216855.1 2614693 D 83332 CDS YP_177869.1 57116973 888961 complement(2617667..2618908) 1 NC_000962.3 Rv2340c, (MTCY98.09c), len: 413 aa. PE_PGRS39,Member of the Mycobacterium tuberculosis PE_family, PGRS subfamily of gly-rich proteins (see citations below),similar to others eg YI18_MYCTU|Q50615|Rv1818c|MTCY1A11.25 PE-PGRS family protein from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 710, E(): 1.4e-22, (41.0% identity in 368 aa overlap); O53884|Rv0872v|MTV043.65c PGRS-family protein from Mycobacterium tuberculosis (606 aa), FASTA scores: opt: 708, E(): 1.9e-22, (42.4% identity in 389 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; PE-PGRS family protein PE_PGRS39 2618908 PE_PGRS39 888961 PE_PGRS39 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS39 YP_177869.1 2617667 R 83332 CDS NP_216857.1 15609478 886275 2619597..2620016 1 NC_000962.3 Rv2341, (MTCY98.10), len: 139 aa. Probable lppQ,conserved lipoprotein, showing some similarity with Rv1228|O33224|LPQX|MTCI61.11 from Mycobacterium tuberculosis (185 aa), FASTA scores: opt: 155; E(): 0.0073; (31.9% identity in 116 aa overlap). Also shows few similarity with P29228|VLPA_MYCHR variant surface antigen a precursor from Mycoplasma hyorhinis (157 aa), FASTA scores: opt: 96, E(): 7.3, (23.1% identity in 143 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LppQ 2620016 lppQ 886275 lppQ Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppQ NP_216857.1 2619597 D 83332 CDS NP_216858.1 15609479 888951 2620272..2620529 1 NC_000962.3 Rv2342, (MTCY98.11), len: 85 aa. Conserved hypothetical protein, highly similar to Q9CCG1|ML0834 hypothetical protein from Mycobacterium leprae (100 aa),FASTA scores: opt: 392, E(): 2.9e-20, (78.2% identity in 78 aa overlap). N-terminus highly similar to N-terminal part of Q9L085|SCC24.32 putative secreted protein from Streptomyces coelicolor (108 aa), FASTA scores: opt: 122,E(): 0.077, (39.15% identity in 46 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2620529 888951 Rv2342 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216858.1 2620272 D 83332 CDS NP_216859.1 15609480 885996 complement(2620533..2622452) 1 NC_000962.3 Rv2343c, (MTCY98.12c), len: 639 aa. Probable dnaG,DNA primase, equivalent to O52200|PRIM_MYCSM|DNAG DNA primase from Mycobacterium smegmatis (636 aa), FASTA scores: opt: 3504, E(): 5.5e-202, (81.55% identity in 639 aa overlap); and Q9CCG2|DNAG|ML0833 DNA primase from Mycobacterium leprae (642 aa), FASTA scores: opt: 3443,E(): 2.5e-198, (80.4% identity in 642 aa overlap). Also highly similar to many DNA primases e.g. Q9S1N4|PRIM_STRCO|DNAG|SC7A8.07c from Streptomyces coelicolor (641 aa), FASTA scores: opt: 1899, E(): 5.1e-106, (47.9% identity in 643 aa overlap); P74893|PRIM_SYNP7|DNAG from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (616 aa), FASTA scores: opt: 860, E(): 6.6e-44, (35.3% identity in 513 aa overlap); P05096|PRIM_BACSU from Bacillus subtilis (603 aa) FASTA scores: opt: 800, E(): 2.5e-40, (33.7% identity in 430 aa overlap); etc.; Probable DNA primase DnaG 2622452 dnaG 885996 dnaG Mycobacterium tuberculosis H37Rv Probable DNA primase DnaG NP_216859.1 2620533 R 83332 CDS NP_216860.1 15609481 885421 complement(2622457..2623752) 1 NC_000962.3 Rv2344c, (MT2409, MTCY98.13c), len: 431 aa. Probable dgt, deoxyguanosine triphosphate triphosphohydrolase,equivalent to Q9CCG3|DGT|ML0831 putative deoxyguanosine triphosphate triphosphohydrolase from Mycobacterium leprae (429 aa), FASTA scores: opt: 2316, E(): 1.6e-137, (83.85% identity in 421 aa overlap); and O52199|DGTP_MYCSM|AF027507_2 deoxyguanosinetriphosphate triphosphohydrolase from Mycobacterium smegmatis (428 aa),FASTA scores: opt: 1991, E(): 3.4e-117, (73.5% identity in 422 aa overlap). Also highly similar or similar to several deoxyguanosine triphosphate hydrolases e.g. Q9L2E9|SC7A8.09c putative deoxyguanosinetriphosphate triphosphohydrolase from Streptomyces coelicolor (424 aa),FASTA scores: opt: 1216, E(): 1e-68, (51.05% identity in 425 aa overlap); BAB48544|MLL1093 dGTP triphosphohydrolase from Rhizobium loti (Mesorhizobium loti) (404 aa), FASTA scores: opt: 489, E(): 3.1e-23, (33.85% identity in 387 aa overlap); P15723|DGTP_ECOLI|DGT|B0160 from Escherichia coli strain K12 (504 aa), FASTA scores: opt: 173, E(): 0.0022,(31.65% identity in 259 aa overlap); etc. Belongs to the dGTPase family.; Probable deoxyguanosine triphosphate triphosphohydrolase Dgt (dGTPase) (dGTP triphosphohydrolase) 2623752 dgt 885421 dgt Mycobacterium tuberculosis H37Rv Probable deoxyguanosine triphosphate triphosphohydrolase Dgt (dGTPase) (dGTP triphosphohydrolase) NP_216860.1 2622457 R 83332 CDS NP_216861.1 15609482 888960 2623821..2625803 1 NC_000962.3 Rv2345, (MTCY98.14), len: 660 aa. Possible conserved transmembrane protein, with hydrophobic stretch at N-terminal end around position 180. Similar to O52198 hypothetical 21.2 KDA protein (fragment) from Mycobacterium smegmatis (195 aa), FASTA scores: opt: 589, E(): 1.5e-23; (47.2% identity in 195 aa overlap).; Possible conserved transmembrane protein 2625803 888960 Rv2345 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_216861.1 2623821 D 83332 CDS NP_216862.1 15609483 888956 complement(2625888..2626172) 1 NC_000962.3 Rv2346c, (MT2411, MTCY98.15c), len: 94 aa. EsxO,ESAT-6 like protein (see citation below), member of Mycobacterium tuberculosis protein family with O53942|Rv1793|MTV049.15, O05300|Rv1198|MTCI364.10,MTCY15C10.33, P96364|MTCY07H7B.03|Rv1037c|MTCY10G2.12,MTCI364.10, etc. Belongs to the ESAT6 family.; Putative ESAT-6 like protein EsxO (ESAT-6 like protein 6) 2626172 esxO 888956 esxO Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxO (ESAT-6 like protein 6) NP_216862.1 2625888 R 83332 CDS NP_216863.1 15609484 886002 complement(2626223..2626519) 1 NC_000962.3 Rv2347c, (MT2412, MTCY98.16c), len: 98 aa. EsxP,ESAT-6 like protein (see citation below). Member of M. tuberculosis hypothetical QILSS protein family with Rv1197,Rv1792, Rv1038c and Rv3620c. Belongs to the ESAT6 family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Putative ESAT-6 like protein EsxP (ESAT-6 like protein 7) 2626519 esxP 886002 esxP Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxP (ESAT-6 like protein 7) NP_216863.1 2626223 R 83332 CDS NP_216864.1 15609485 886006 complement(2626654..2626980) 1 NC_000962.3 Rv2348c, (MTCY98.17c), len: 108 aa. Hypothetical unknown protein.; Hypothetical protein 2626980 886006 Rv2348c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216864.1 2626654 R 83332 CDS NP_216865.1 15609486 886000 complement(2627172..2628698) 1 NC_000962.3 Rv2349c, (MT2414, MTCY98.18c), len: 508 aa. Probable plcC, phospolipase C 3 (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1013, E(): 9.3e-54, (38.85% identity in 525 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 630, E(): 1.5e-30, (35.15% identity in 535 aa overlap); Q9S816|T12J13.18|T21P5.4 putative phospholipase from Arabidopsis thaliana (Mouse-ear cress) (521 aa), FASTA scores: opt: 218, E(): 1e-05, (27.05% identity in 451 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2497, E(): 9e-144, (68.35% identity in 509 aa overlap); Q50560|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 (520 aa), FASTA scores: opt: 2494, E(): 1.4e-143, (68.1% identity in 514 aa overlap); P95246|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (512 aa), FASTA scores: opt: 2474, E(): 2.2e-142, (67.65% identity in 513 aa overlap); etc. Belongs to the bacterial phospholipase C family.; Probable phospholipase C 3 PlcC 2628698 plcC 886000 plcC Mycobacterium tuberculosis H37Rv Probable phospholipase C 3 PlcC NP_216865.1 2627172 R 83332 CDS NP_216866.1 15609487 885999 complement(2628781..2630319) 1 NC_000962.3 Rv2350c, (MT2415, MTCY98.19c), len: 512 aa. plcB (alternate gene name: mpcB), membrane-associated phospolipase C 2 (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 885, E(): 2.3e-44, (38.5% identity in 525 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 639, E(): 6.3e-30, (537 aa overlap); Q9RGS8 non-hemolytic phospholipase C from Pseudomonas aeruginosa (700 aa), FASTA scores: opt: 864, E(): 3.9e-43, (39.2% identity in 528 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q50560|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 (520 aa), FASTA scores: opt: 2788, E(): 4.5e-156, (75.5% identity in 514 aa overlap); Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2623, E(): 2.1e-146, (71.5% identity in 512 aa overlap); P95245|PLCC|Rv2349c|MT2414|MTCY98.18c phospholipase C 3 (508 aa), FASTA scores: opt: 2474, E(): 1.1e-137, (67.65% identity in 513 aa overlap); etc. Belongs to the bacterial phospholipase C family. Supposed membrane-associated, at the extracellular side. Substrate of Tat pathway (See McDonough et al., 2008).; Membrane-associated phospholipase C 2 PlcB 2630319 plcB 885999 plcB Mycobacterium tuberculosis H37Rv Membrane-associated phospholipase C 2 PlcB NP_216866.1 2628781 R 83332 CDS NP_216867.1 15609488 885995 complement(2630537..2632075) 1 NC_000962.3 Rv2351c, (MTP40, MT2416, MTCY98.20c), len: 512 aa. plcA (alternate gene name: mpcA), membrane-associated phospolipase C 1 (MTP40 antigen) (see citations below),similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1064, E(): 4.3e-55, (39.85% identity in 517 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 562, E(): 1.6e-25, (35.35% identity in 481 aa overlap); Q9RGS8|PLCN|PHLN_BURPS non-hemolytic phospholipase C from Burkholderia pseudomallei (Pseudomonas pseudomallei) (700 aa), FASTA scores: opt: 843, E(): 4.4e-42, (40.5% identity in 531 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P95246|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (512 aa), FASTA scores: opt: 2788, E(): 1.2e-156, (75.5% identity in 514 aa overlap) (alias Q50561|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (521 aa), FASTA scores: opt: 2700, E(): 1.8e-151, (73.8% identity in 515 aa overlap)); Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2643, E(): 4.1e-148, (71.6% identity in 511 aa overlap); etc. Belongs to the bacterial phospholipase C family. Supposed membrane-associated, at the extracellular side.; Membrane-associated phospholipase C 1 PlcA (MTP40 antigen) 2632075 plcA 885995 plcA Mycobacterium tuberculosis H37Rv Membrane-associated phospholipase C 1 PlcA (MTP40 antigen) NP_216867.1 2630537 R 83332 CDS YP_177870.1 57116974 888959 complement(2632923..2634098) 1 NC_000962.3 Rv2352c, (MTCY98.21c), len: 391 aa. PPE38, Member of Mycobacterium tuberculosis PPE_family, highly similar to many e.g. Q10778|MTCY48.17|Y04H_MYCTU (734 aa), FASTA scores: opt: 713, E(): 2.8e-27, (37.7% identity in 430 aa overlap); Q10540|MTCY31.06c, Q11031|MTCY02B10.25c,Q10813|MTCY274.23c, P42611|MTV037.06C, P71868|MTCY03C7.23,P95248|MTCY98.22c, P71869|MTCY03C7.24c, etc.; PPE family protein PPE38 2634098 PPE38 888959 PPE38 Mycobacterium tuberculosis H37Rv PPE family protein PPE38 YP_177870.1 2632923 R 83332 CDS YP_177871.1 57116975 886003 complement(2634528..2635592) 1 NC_000962.3 Rv2353c, (MTCY98.22c), len: 354 aa. PPE39, Member of Mycobacterium tuberculosis PPE family, highly similar to many e.g. near ORF P95249|Rv2356c|MTCY98.25 from Mycobacterium tuberculosis (615 aa), FASTA scores: opt: 1566, E(): 3.2e-69, (66.1% identity in 349 aa overlap); Q10778|MTCY48.17, Q10540|MTCY31.06c, E241779|MTCY98,Q10813|MTCY274.23c, P71868|MTCY03C7.23,P71869|MTCY03C7.24c, P42611|MTV037.06C, E64997|MTCY98,Q10707|MTCY49.38C, P71657|MTCY02B10.25c, etc. Note that the ATG and RBS appear to be provided by the IR of neighbouring IS6110. Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE39 2635592 PPE39 886003 PPE39 Mycobacterium tuberculosis H37Rv PPE family protein PPE39 YP_177871.1 2634528 R 83332 CDS NP_216870.1 15609491 888963 2635628..2635954 1 NC_000962.3 Rv2354, (MTCY98.23), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2354 and Rv2355,the sequence UUUUAAAG (directly upstream of Rv2355) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Probable transposase for insertion sequence element IS6110 (fragment) 2635954 888963 Rv2354 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS6110 (fragment) NP_216870.1 2635628 D 83332 CDS NP_216871.2 448824783 888957 <2635903..2636889 1 NC_000962.3 Rv2355, (MTCY98.24), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2354 and Rv2355, the sequence UUUUAAAG (directly upstream of Rv2355) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa).; Probable transposase 2636889 888957 Rv2355 Mycobacterium tuberculosis H37Rv Probable transposase NP_216871.2 <2635903 D 83332 CDS YP_177872.1 57116976 888950 complement(2637688..2639535) 1 NC_000962.3 Rv2356c, (MTCY98.25), len: 615 aa. PPE40, Member of Mycobacterium tuberculosis PPE_family, highly similar to others e.g. Q10778|MTCY48.17|YF48_MYCTU hypothetical PPE-family protein (678 aa), FASTA scores: opt: 1888, E(): 1.9e-78, (54.4% identity in 667 aa overlap); Q10540|MTCY31.06c, E241779|MTCY98, P42611|MTV037.06c,Q10813|MTCY274.23c, P71657|MTCY02B10.25c, MTCY03C7.23,P71869|MTCY03C7.24c, etc. Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE40 2639535 PPE40 888950 PPE40 Mycobacterium tuberculosis H37Rv PPE family protein PPE40 YP_177872.1 2637688 R 83332 CDS NP_216873.1 15609494 888962 complement(2639673..2641064) 1 NC_000962.3 Rv2357c, (MTCY27.23, MTCY98.26), len: 463 aa. Probable glyS, glycyl-tRNA synthetase, equivalent to Q9CCG4|GLYS|ML0826 putative glycyl-tRNA synthase from Mycobacterium leprae (463 aa), FASTA scores: opt: 2898,E(): 1e-179, (90.2% identity in 459 aa overlap). Also highly similar to others e.g. Q9L2H9|SYG_STRCO|SCC121.07c from Streptomyces coelicolor (460 aa), FASTA scores: opt: 2210, E(): 2.9e-135, (68.3% identity in 457 aa overlap); Q9PPZ7|SYG_UREPA|GLYS|UU493 glycyl-tRNA synthetase from Ureaplasma parvum (Ureaplasma urealyticum biotype 1) (473 aa), FASTA scores: opt: 1254, E(): 1.7e-73, (45.25% identity in 462 aa overlap); P75425|SYG_MYCPN|GLYS|MPN354|MP482 glycyl-tRNA synthetase from Mycoplasma pneumoniae (449 aa), FASTA scores: opt: 1074, E(): 6.9e-62, (39.45% identity in 454 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family.; Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS) 2641064 glyS 888962 glyS Mycobacterium tuberculosis H37Rv Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS) NP_216873.1 2639673 R 83332 CDS NP_216874.1 15609495 888965 2641246..2641653 1 NC_000962.3 Rv2358, (MTCY27.22c), len: 135 aa. Probable smtB,transcriptional regulator, arsR family, equivalent to Q9CCG5|ML0825 putative ArsR-family transcriptional regulator from Mycobacterium leprae (140 aa), FASTA scores: opt: 647, E(): 2e-34, (72.9% identity in 140 aa overlap). Also similar to others e.g. BAB48273|MLR0745 Transcriptional regulator from Rhizobium loti (Mesorhizobium loti) (104 aa), FASTA scores: opt: 185, E(): 3.4e-05, (43.25% identity in 74 aa overlap) (has its N-terminus shorter); P15905|ARR1_ECOLI arsenical resistance operon repressor from Escherichia coli (117 aa), FASTA scores: opt: 164, E(): 8.1e-05, (39.1% identity in 69 aa overlap); etc. Also similar to O53838|Rv0827|MTV043.19c putative transcriptional regulator from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 201, E(): 4e-06,(35.7% identity in 98 aa overlap); and O69711|Rv3744|MTV025.092 putative regulatory protein from Mycobacterium tuberculosis (120 aa), FASTA scores: opt: 209, E(): 1.2e-06, (35.5 % identity in 93 aa overlap). Contains possible helix-turn-helix motif at aa 72-93 (Score 1103, +2.94 SD). Belongs to the ArsR family of transciptional regulators. Shown to bind palindromic DNA sequence upstream of Rv2358; inhibited by Zn2+ (See Canneva et al., 2005).; Probable transcriptional regulatory protein SmtB (probably ArsR-family) 2641653 smtB 888965 smtB Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein SmtB (probably ArsR-family) NP_216874.1 2641246 D 83332 CDS NP_216875.1 15609496 886009 2641650..2642042 1 NC_000962.3 Rv2359, (MTCY27.21c), len: 130 aa. Probable zur,zinc uptake regulation protein, equivalent to FURB|ML0824|Q9CCG6 putative ferric uptake regulatory protein from Mycobacterium leprae (131 aa), FASTA scores: opt: 765, E(): 1.7e-43, (86.9% identity in 130 aa overlap). Also highly similar to ferric uptake regulation proteins e.g. Q9L2H5|SCC121.11 putative metal uptake regulation protein from Streptomyces coelicolor (139 aa), FASTA scores: opt: 547, E(): 3.4e-29, (59.4% identity in 133 aa overlap); P06975|FUR_ECOLI from Escherichia coli (148 aa),FASTA scores: opt: 322, E(): 1.9e-14, (37.9% identity in 132 aa overlap); P45599|FUR_KLEPN ferric uptake regulation protein from Klebsiella pneumoniae (155 aa), FASTA scores: opt: 314, E(): 6.7e-14, (36.35% identity in 132 aa overlap); etc. Belongs to the fur/ZUR family. Note that previously known as furB.; Probable zinc uptake regulation protein Zur 2642042 zur 886009 zur Mycobacterium tuberculosis H37Rv Probable zinc uptake regulation protein Zur NP_216875.1 2641650 D 83332 CDS NP_216876.1 15609497 888952 complement(2642150..2642578) 1 NC_000962.3 Rv2360c, (MTCY27.20), len: 142 aa. Unknown protein.; hypothetical protein 2642578 888952 Rv2360c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216876.1 2642150 R 83332 CDS NP_216877.1 15609498 888964 complement(2642578..2643468) 1 NC_000962.3 Rv2361c, (MT2430, MTCY27.19), len: 296 aa. Long (C50) chain Z-isoprenyl diphosphate synthase (see citation below), equivalent to UPPS_MYCLE|ML0634|B1937_F2_65|P38119 undecaprenyl pyrophosphate synthetase from Mycobacterium leprae (296 aa), FASTA scores: opt: 1789, E(): 1.8e-97,(86.5% identity in 296 aa overlap). Also highly similar to others e.g. UPPS|Q9L2H4 undecaprenyl pyrophosphate synthetase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 1098, E(): 8.2e-60, (63.5% identity in 247 aa overlap); Q55482|UPPS_SYNY3|SLL0506 from Synechocystis sp. strain PCC 6803 (249 aa), FASTA scores: opt: 686, E(): 4.2e-33, (46.4% identity in 235 aa overlap); O67291|UPPS_AQUAE|AQ_1248 from Aquifex aeolicus (231 aa),FASTA scores: opt: 684, E(): 5.2e-33, (46.3% identity in 229 aa overlap); etc. Also similar to Rv1086|MTV017.39 from Mycobacterium tuberculosis. Contains PS01066 Hypothetical YBR002c family signature. Seems to belong to the UPP synthetase family. Note that previously known as uppS.; Long (C50) chain Z-isoprenyl diphosphate synthase (Z-decaprenyl diphosphate synthase) 2643468 888964 Rv2361c Mycobacterium tuberculosis H37Rv Long (C50) chain Z-isoprenyl diphosphate synthase (Z-decaprenyl diphosphate synthase) NP_216877.1 2642578 R 83332 CDS NP_216878.1 15609499 888954 complement(2643461..2644258) 1 NC_000962.3 Rv2362c, (MTCY27.18), len: 265 aa. RecO, DNA repair protein, equivalent to Q9CCN0|ML0633 Mycobacterium leprae Hypothetical protein (268 aa), FASTA scores: opt: 1560,E(): 8.5e-93, (86.6% identity in 268 aa overlap). Also highly similar to others e.g. Q9L2H3|SCC121.13c DNA repair protein recO from Streptomyces coelicolor (251 aa), FASTA scores: opt: 843, E(): 6.9e-47, (52.2% identity in 249 aa overlap); and similar to other hypothetical proteins. Weak similarity with P42095|RECO_BACSU DNA repair protein recombinase from Bacillus subtilis (255 aa), FASTA scores: opt: 270, E(): 3.6e-10, (26.4% identity in 182 aa overlap). Maybe involved in modulating assembly and disassembly of RECA filaments (with RECF|Rv0003 and RECR|Rv3715c) (see citation below). Contains match to Pfam entry PF02565 Recombination protein O. Belongs to the RECO family.; Possible DNA repair protein RecO 2644258 recO 888954 recO Mycobacterium tuberculosis H37Rv Possible DNA repair protein RecO NP_216878.1 2643461 R 83332 CDS NP_216879.1 15609500 888955 2644320..2645774 1 NC_000962.3 Rv2363, (MTCY27.17c), len: 484 aa. Probable amiA2,amidase, highly similar or similar to others e.g. O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 777,E(): 1.1e-38, (35.0% identity in 474 aa overlap); Q55424|AMID_SYNY3|SLL0828 putative amidase from Synechocystis sp. strain PCC 6803 (506 aa), FASTA scores: opt: 770, E(): 3e-38, (36.4% identity in 456 aa overlap); Q53116|AMDA enantiomerase-selective amidase from Rhodococcus sp. (462 aa), FASTA scores: opt: 701, E(): 3.5e-34, (32.7% identity in 468 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. AMI2_MYCTU|AMIB2|Q11056|Rv1263|MT1301|MTCY50.19c|cy50.19c amidase (462 aa), FASTA scores: opt: 1141, E(): 2.9e-60,(45.4% identity in 454 aa overlap); etc. Contains PS00571 Amidases signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the amidase family.; Probable amidase AmiA2 (aminohydrolase) 2645774 amiA2 888955 amiA2 Mycobacterium tuberculosis H37Rv Probable amidase AmiA2 (aminohydrolase) NP_216879.1 2644320 D 83332 CDS YP_177873.1 57116977 886027 complement(2645771..2646673) 1 NC_000962.3 Rv2364c, (MT2433, MTCY27.16), len: 300 aa. Probable era, GTP-binding protein, equivalent to Q49768|ERA_MYCLE|ML0631|B1937_F3_102 GTP-binding protein era homolog from Mycobacterium leprae (300 aa) FASTA scores: opt: 1589, E(): 3.4e-88, (81.4% identity in 301 aa overlap). Also highly similar to other GTP-binding proteins e.g. Q9RDF2|ERA_STRCO|SCC77.06 from Streptomyces coelicolor (317 aa), FASTA scores: opt: 1264, E(): 1.1e-68, (64.0% identity in 306 aa overlap); Q9KD52|ERA_BACHD|BH1367|BEX from Bacillus halodurans (304 aa), FASTA scores: opt: 869,(44.8% identity in 297 aa overlap); Q9KIH7|ERA_LACLA|ERAL from Lactococcus lactis (subsp. lactis) (Streptococcus lactis), and Lactococcus lactis (subsp. cremoris) (Streptococcus cremoris) (303 aa), FASTA scores: opt: 781,E(): 9.4e-40, (40.25% identity in 298 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the era/TRME family of GTP-binding proteins, era subfamily. Note that previously known as bex.; Probable GTP-binding protein Era 2646673 era 886027 era Mycobacterium tuberculosis H37Rv Probable GTP-binding protein Era YP_177873.1 2645771 R 83332 CDS NP_216881.1 15609502 885259 complement(2646747..2647088) 1 NC_000962.3 Rv2365c, (MTCY27.15), len: 113 aa. Conserved hypothetical protein, highly similar to Q49767|ML0630|B1937_F3_101|CAC30138 Hypothetical protein from Mycobacterium leprae (108 aa), FASTA scores: opt: 426,E(): 1.4e-18, (67.9% identity in 106 aa overlap). Also highly similar to Q9RDF3|SCC77.05 from Streptomyces coelicolor (132 aa), FASTA scores: opt: 254, E(): 1.9e-18,(53.1% identity in 96 aa overlap). Equivalent to AAK46728 from Mycobacterium tuberculosis strain CDC1551 (93 aa) but longer 20 aa.; hypothetical protein 2647088 885259 Rv2365c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216881.1 2646747 R 83332 CDS NP_216882.1 15609503 885987 complement(2647060..2648367) 1 NC_000962.3 Rv2366c, (MTCY27.14), len: 435 aa. Probable conserved transmembrane protein, highly similar to Q9L2L3|SCC117.07 putative membrane protein from Streptomyces coelicolor (358 aa), FASTA scores: opt: 1159,E(): 5.5e-64, (53.0% identity in 353 aa overlap); ans similar to hypothetical proteins and hemolysin-related proteins e.g. Q9HN02|HLP|VNG2308G hemolysin protein from Halobacterium sp. strain NRC-1 (457 aa), FASTA scores: opt: 623, E(): 6.2e-31, (28.4% identity in 433 aa overlap); etc. Potential transmembrane protein with 2 CBS domains. Belongs to the UPF0053 family.; Probable conserved transmembrane protein 2648367 885987 Rv2366c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216882.1 2647060 R 83332 CDS NP_216883.1 15609504 885989 complement(2648364..2648912) 1 NC_000962.3 Rv2367c, (MTCY27.13), len: 182 aa. Conserved hypothetical protein, equivalent to Q49752|YN67_MYCLE|ML0628|B1937_F1_21 hypothetical 19.8 KDA protein from Mycobacterium leprae (178 aa), FASTA scores: opt: 1051, E(): 2e-59, (89.1% identity in 175 aa overlap). Also highly similar to others e.g. Q9L2L4|SCC117.06 conserved hypothetical protein from Streptomyces coelicolor (165 aa), FASTA scores: opt: 599, E(): 6e-31, (56.5% identity in 154 aa overlap); Q9KD56|BH1363 hypothetical protein from Bacillus halodurans (159 aa), FASTA scores: opt: 311, E(): 8.3e-13, (45.05% identity in 111 aa overlap); etc.; hypothetical protein 2648912 885989 Rv2367c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216883.1 2648364 R 83332 CDS YP_177874.1 57116978 885998 complement(2648916..2649974) 1 NC_000962.3 Rv2368c, (MTCY27.12), len: 352 aa. Probable phoH1,phoH-like protein (phosphate starvation-induced protein),probably ATP-binding protein, equivalent to Q49751|PHOL_MYCLE| ML0627|B1937_F1_20 PHOH-like protein from Mycobacterium leprae (349 aa), FASTA scores: opt: 1952, E(): 4.7e-107, (88.9% identity in 352 aa overlap). Also highly similar to Q9L2L5|SCC117.05 PHOH-like protein from Streptomyces coelicolor (359 aa), FASTA scores: opt: 1407, E(): 3.6e-75, (63.6% identity in 349 aa overlap); Q9RSY1|DR1988 PHOH-related protein from Deinococcus radiodurans (380 aa), FASTA scores: opt: 1053, E(): 1.9e-54, (53.3% identity in 349 aa overlap); Q9KD58|PHOH|BH1361 phosphate starvation-induced protein from Bacillus halodurans (320 aa), FASTA scores: opt: 1019,E(): 1.6e-52, (54.35% identity in 300 aa overlap); P46343|PHOL_BACSU PHOH-like protein from Bacillus subtilis (319 aa), FASTA scores: opt: 1014, E(): 3.2e-52, (50.8% identity in 303 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the PHOH family. Note that previously known as phoH.; Probable PHOH-like protein PhoH1 (phosphate starvation-inducible protein PSIH) 2649974 phoH1 885998 phoH1 Mycobacterium tuberculosis H37Rv Probable PHOH-like protein PhoH1 (phosphate starvation-inducible protein PSIH) YP_177874.1 2648916 R 83332 CDS NP_216885.1 15609506 885811 complement(2649946..2650248) 1 NC_000962.3 Rv2369c, (MTCY27.11), len: 100 aa. Hypothetical unknown protein.; Hypothetical protein 2650248 885811 Rv2369c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216885.1 2649946 R 83332 CDS NP_216886.1 15609507 886017 complement(2650245..2651558) 1 NC_000962.3 Rv2370c, (MTCY27.10), len: 437 aa. Conserved hypothetical protein, member of family proteins from Mycobacterium tuberculosis with Rv1453|MTCY493_01c|O06807 conserved hypothetical protein from Mycobacterium tuberculosis (432 aa), FASTA scores: opt: 1943, E(): 9.4e-115, (69.9% identity in 409 aa overlap); Rv1194c|MTCI364.06c; etc. Also similar to AAK45764|MT1500 conserved hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (432 aa), FASTA scores: opt: 1934, E(): 9.4e-115, (69.9% identity in 409 aa overlap).; hypothetical protein 2651558 886017 Rv2370c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216886.1 2650245 R 83332 CDS YP_177875.1 57116979 885141 2651753..2651938 1 NC_000962.3 Rv2371, (MTCY27.09c), len: 61 aa. PE_PGRS40, Short protein, member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to N-terminal part of others e.g. AAK44356|MT0132 PE_PGRS family protein from Mycobacterium tuberculosis strain CDC1551 (561 aa), FASTA scores: opt: 217, E(): 4.9e-08, (69.65% identity in 56 aa overlap); etc.; PE-PGRS family protein PE_PGRS40 2651938 PE_PGRS40 885141 PE_PGRS40 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS40 YP_177875.1 2651753 D 83332 CDS NP_216888.1 15609509 885926 complement(2652037..2652825) 1 NC_000962.3 Rv2372c, (MTCY27.08), len: 262 aa. Conserved hypothetical protein, equivalent to Q9CCN1|ML0626 hypothetical protein from Mycobacterium leprae (257 aa),FASTA scores: opt: 1277, E(): 3e-71, (77.25% identity in 255 aa overlap). Also highly similar to others e.g. Q9RDD9|SDRD hypothetical 26.1 KDA protein from Streptomyces coelicolor (249 aa), FASTA scores: opt: 624, E(): 3.2e-31,(45.05% identity in 253 aa overlap); P54461|YQEU_BACSU hypothetical 28.8 kDa protein from Bacillus subtilis (256 aa), FASTA scores: opt: 375, E(): 6e-16, (32.5% identity in 234 aa overlap); etc. C-terminal half highly similar to Q49763|B1937_F2_57 from Mycobacterium leprae (128 aa),FASTA scores: opt: 577, E(): 1.4e-28, (75.8% identity in 124 aa overlap). Belongs to the UPF0088 family.; hypothetical protein 2652825 885926 Rv2372c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216888.1 2652037 R 83332 CDS NP_216889.1 15609510 886023 complement(2652839..2653987) 1 NC_000962.3 Rv2373c, (MTCY27.07), len: 382 aa. Probable dnaJ2,chaperone protein, equivalent to Q49762|DNJ2_MYCLE|ML0625|B1937_F2_56 chaperone protein from Mycobacterium leprae (378 aa), FASTA scores: opt: 2301,E(): 1.7e-120, (87.5% identity in 382 aa overlap). Also highly similar to other chaperone proteins DNAJ/DNAJ2 e.g. Q9RDD7|DNJ2_STRCO|SCC77.21c from Streptomyces coelicolor (378 aa), FASTA scores: opt: 1456, E(): 1.2e-73, (54.8% identity in 385 aa overlap); O52164|DNJ2_STRAL from Streptomyces albus (379 aa) FASTA scores: opt: 1378, E(): 2.6e-69, (52.2% identity in 385 aa overlap); Q9S5A3|DNAJ_LISMO from Listeria monocytogenes (377 aa),FASTA scores: opt: 1013, E(): 4.6e-49, (41.3% identity in 385 aa overlap); etc. Also similar to Rv0352|MTCY13E10.12 from Mycobacterium tuberculosis. Contains 1 J domain and 1 cr domain. Belongs to the DNAJ family.; Probable chaperone protein DnaJ2 2653987 dnaJ2 886023 dnaJ2 Mycobacterium tuberculosis H37Rv Probable chaperone protein DnaJ2 NP_216889.1 2652839 R 83332 CDS NP_216890.1 15609511 885924 complement(2654062..2655093) 1 NC_000962.3 Rv2374c, (MTCY27.06), len: 343 aa. Probable hrcA,heat-inducible transcriptional repressor (see citation below), equivalent to Q9CCN2|HRCA|ML0624 putative heat-inducible transcriptional regulator from Mycobacterium leprae (343 aa), FASTA scores: opt: 1926, E(): 3.9e-107,(89.8% identity in 343 aa overlap). Also highly similar to other heat-inducible transcription repressor proteins e.g. Q9RDD6|HRCA|SCC77.22c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 1227, E(): 1.1e-65, (58.8% identity in 335 aa overlap); O52163|HRCA_STRAL from Streptomyces albus (338 aa), FASTA scores: opt: 1196, E(): 7.7e-64,(56.1% identity in 335 aa overlap); P25499|HRCA_BACSU heat-inducible transcription repressor from Bacillus subtilis (343 aa), FASTA scores: opt: 538, E(): 8.4e-25,(28.9% identity in 325 aa overlap); etc. Almost identical,but conflict at C-terminus, to Q49749|YGRP|B1937_F1_18 putative heat-inducible transcription repressor from Mycobacterium leprae (197 aa) FASTA scores: opt: 1126, E(): 6.9e-60, (91.8% identity in 195 aa overlap). Belongs to the HRCA family.; Probable heat shock protein transcriptional repressor HrcA 2655093 hrcA 885924 hrcA Mycobacterium tuberculosis H37Rv Probable heat shock protein transcriptional repressor HrcA NP_216890.1 2654062 R 83332 CDS NP_216891.1 15609512 885520 2655265..2655582 1 NC_000962.3 Rv2375, (MTCY27.05c), len: 105 aa. Conserved hypothetical protein, highly similar to only CAC32314|2SCD60.09c conserved hypothetical protein from Streptomyces coelicolor (98 aa), FASTA scores: opt: 425,E(): 5.7e-24, (63.25% identity in 98 aa overlap).; hypothetical protein 2655582 885520 Rv2375 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216891.1 2655265 D 83332 CDS NP_216892.1 15609513 885515 complement(2655609..2656115) 1 NC_000962.3 Rv2376c, (MT2445, MTCY27.04), len: 168 aa. Cfp2 (alternate gene name: mtb12), low molecular weight antigen,secreted protein similar to Q49771|MB12_MYCLE|ML0620|B1937_F3_91 low molecular weight antigen MTB12 homolog precursor from Mycobacterium leprae (167 aa), FASTA scores: opt: 682, E(): 1.7e-32, (65.5% identity in 165 aa overlap). Belongs to the MTB12 family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Low molecular weight antigen CFP2 (low molecular weight protein antigen 2) (CFP-2) 2656115 cfp2 885515 cfp2 Mycobacterium tuberculosis H37Rv Low molecular weight antigen CFP2 (low molecular weight protein antigen 2) (CFP-2) NP_216892.1 2655609 R 83332 CDS NP_216893.1 15609514 885968 complement(2656215..2656430) 1 NC_000962.3 Rv2377c, (MT2445.1, MTCY27.03), len: 71 aa. Putative mbtH, conserved protein with no function assigned (see Quadri et al., 1998; De Voss et al., 1999), similar to hypothetical proteins or proteins found in several gene clusters for biosynthesis or transport of siderophores and other nonribosomally synthesized peptides e.g. Q9Z388|SCE8.11c putative small conserved hypothetical protein from Streptomyces coelicolor (71 aa), FASTA scores: opt: 345, E(): 1.4e-19, (68.2% identity in 66 aa overlap); Q9F8V3|CUMB COUY protein (probably involved in the biosynthesis of aminocoumarin antibiotic coumermycin a(1)) (see Wang et al., 2000) from Streptomyces rishiriensis (71 aa), FASTA scores: opt: 329, E(): 2.2e-18, (63.2% identity in 68 aa overlap); Q9F5J2|SIM-CB MBTH-like protein (probably protein involved in the biosynthesis of aminocoumarin antibiotic coumermycin a(1)) from Streptomyces antibioticus (70 aa), FASTA scores: opt: 308,E(): 8.4e-17, (65.6% identity in 64 aa overlap); Q9FB14 MBTH-like protein (involved in the biosynthesis of the antitumor drug bleomycin) (see Du et al., 2000) from Streptomyces verticillus FASTA scores: opt: 220, E(): 8.8e-10, (41.2% identity in 68 aa overlap); etc.; Putative conserved protein MbtH 2656430 mbtH 885968 mbtH Mycobacterium tuberculosis H37Rv Putative conserved protein MbtH NP_216893.1 2656215 R 83332 CDS NP_216894.1 15609515 885648 complement(2656408..2657703) 1 NC_000962.3 Rv2378c, (MTCY27.02), len: 431 aa. MbtG,lysine-N-oxygenase (hydroxylase) (EC 1.13.12.10 or 1.14.13.59; depending if enzyme is NADPH dependent or independent) (see citations below), showing some similarity with various proteins including ornithine and lysine-N-oxygenases, e.g. Q9K6Q1|TRKA|BH3677 potassium uptake protein from Bacillus halodurans (350 aa), FASTA scores: opt: 153, E(): 0.016, (25.2% identity in 246 aa overlap); P56584|SID1_USTMA L-ornithine 5-monooxygenase from Ustilago maydis (Smut fungus) (570 aa), FASTA scores: opt: 136, E(): 0.31, (22.85% identity in 127 aa overlap); Q9HHV0|HXYA|VNG6214G monooxygenase from Halobacterium sp. strain NRC-1 (477 aa), FASTA scores: opt: 119, E(): 3.4,(40.0% identity in 70 aa overlap); O69828|SC1A6.23 putative lysine N-hydroxlase (fragment) from Streptomyces coelicolor (134 aa), blast score: 76 (similarity in part for this one); etc. Cofactors: FAD (by similarity).; Lysine-N-oxygenase MbtG (L-lysine 6-monooxygenase) (lysine N6-hydroxylase) 2657703 mbtG 885648 mbtG Mycobacterium tuberculosis H37Rv Lysine-N-oxygenase MbtG (L-lysine 6-monooxygenase) (lysine N6-hydroxylase) NP_216894.1 2656408 R 83332 CDS NP_216895.1 15609516 885874 complement(2657700..2662085) 1 NC_000962.3 Rv2379c, (MTCY27.01), len: 1461 aa. MbtF, peptide synthetase (see citations below), similar in part to several synthases e.g. O52820|PCZA363.4 protein from Amycolatopsis orientalis (4077 aa), FASTA scores: opt: 1873, E(): 1.1e-99, (35.55% identity in 1522 aa overlap); O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 1817, E(): 2.1e-96, (33.65% identity in 1463 aa overlap); O52821 protein similar to peptide synthetase from Amycolatopsis orientalis (1860 aa) FASTA scores: opt: 1705,E(): 2.9e-90, (34.75% identity in 1344 aa overlap); Q9XCF2|PSTB putative peptide synthetase (similar to Mycobacterium tuberculosis nrp protein) from Mycobacterium avium (2552 aa), FASTA scores: opt: 1687, E(): 4e-89,(35.45% identity in 1058 aa overlap); Q9ZET7 peptide synthetase (fragment) from Mycobacterium smegmatis (1438 aa), FASTA scores: opt: 1479, E(): 2.5e-77, (30.45% identity in 1507 aa overlap); etc. Contains PS00455 putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Peptide synthetase MbtF (peptide synthase) 2662085 mbtF 885874 mbtF Mycobacterium tuberculosis H37Rv Peptide synthetase MbtF (peptide synthase) NP_216895.1 2657700 R 83332 CDS NP_216896.3 448824784 885822 complement(2662067..2667115) 1 NC_000962.3 Rv2380c, (MTCY22H8.05), len: 1682 aa. MbtE, peptide synthetase (see citations below), similar in part to several synthases e.g. O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 2635, E(): 1.9e-146, (36.8% identity in 1657 aa overlap); O05647|SNBDE virginiamycin S synthetase (fragment) from Streptomyces virginiae (1997 aa) FASTA scores: opt: 2580, E(): 1.6e-143, (40.65% identity in 1163 aa overlap); Q9R9I2|DHBF protein involved in siderophore production from Bacillus subtilis (2378 aa),FASTA scores: opt: 2388, E(): 3.6e-132, (33.9% identity in 1579 aa overlap); O68487|ACMB actinomycin synthetase II from Streptomyces chrysomallus (2611 aa), FASTA scores: opt: 2165, E(): 4.9e-119, (35.0% identity in 1634 aa overlap); etc. Equivalent to AAK46743 from Mycobacterium tuberculosis strain CDC1551 (1787 aa) but shorter 105 aa. Contains PS00455 putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family.; Peptide synthetase MbtE (peptide synthase) 2667115 mbtE 885822 mbtE Mycobacterium tuberculosis H37Rv Peptide synthetase MbtE (peptide synthase) NP_216896.3 2662067 R 83332 CDS NP_216897.1 15609518 885850 complement(2667255..2670269) 1 NC_000962.3 Rv2381c, (MTCY22H8.04), len: 1004 aa. MbtD,polyketide synthase (see citations below), similar in part to several synthases e.g. Q03132|ERY2_SACER|ERYA erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 971, E(): 1e-46, (29.35% identity in 1043 aa overlap); Q9F829|megaii megalomicin 6-deoxyerythronolide B synthase 2 from Micromonospora megalomicea subsp. nigra (3562 aa), FASTA scores: opt: 787,E(): 2.4e-36, (29.35% identity in 1032 aa overlap); Q9L4W4|NYSB polyketide synthase from Streptomyces noursei (3192 aa), FASTA scores: opt: 761, E(): 6.6e-35, (29.55% identity in 1086 aa overlap); O30764|NIDA1 polyketide synthase modules 1 and 2 from Streptomyces caelestis (4340 aa), FASTA scores: opt: 726, E(): 7.8e-33, (27.3% identity in 1052 aa overlap); etc. Contains PS00012 Phosphopantetheine attachment site.; Polyketide synthetase MbtD (polyketide synthase) 2670269 mbtD 885850 mbtD Mycobacterium tuberculosis H37Rv Polyketide synthetase MbtD (polyketide synthase) NP_216897.1 2667255 R 83332 CDS NP_216898.1 15609519 885908 complement(2670269..2671603) 1 NC_000962.3 Rv2382c, (MTCY22H8.03), len: 444 aa. MbtC,polyketide synthase (see citations below), similar in part to several synthases e.g. Q9F7T9 avermectin polyketide synthase (fragment) from Streptomyces avermitilis (3626 aa), FASTA scores: opt: 1458, E(): 7e-82, (50.65% identity in 446 aa overlap); AAG23264|SPNA polyketide synthase loading and extender module 1 from Saccharopolyspora spinosa (2595 aa) FASTA scores: opt: 1441, E(): 6e-81,(49.1% identity in 446 aa overlap); O33954|TYLG tylactone synthase starter module and modules 1 & 2 from Streptomyces fradiae (4472 aa) FASTA scores: opt: 1439, E(): 1.2e-80,(51.0% identity in 447 aa overlap); O30764|NIDA1 polyketide synthase modules 1 and 2 from Streptomyces caelestis (4340 aa) FASTA scores: opt: 1432, E(): 3.3e-80, (50.9% identity in 442 aa overlap); etc.; Polyketide synthetase MbtC (polyketide synthase) 2671603 mbtC 885908 mbtC Mycobacterium tuberculosis H37Rv Polyketide synthetase MbtC (polyketide synthase) NP_216898.1 2670269 R 83332 CDS NP_216899.1 15609520 885838 complement(2671593..2675837) 1 NC_000962.3 Rv2383c, (MTCY22H8.02), len: 1414 aa. MbtB,phenyloxazoline synthase (see citations below), similar to the N-terminal region of several synthetases e.g. Q9EWP5|SC4C2.17 putative non-ribosomal peptide synthase from Streptomyces coelicolor (2229 aa), FASTA scores: opt: 2878, E(): 4.1e-156, (46.85% identity in 1138 aa overlap); Q9Z399|IRP2 yersiniabactin biosynthetic from Yersinia pestis (2041 aa), FASTA scores: opt: 2297, E(): 5.3e-123,(38.55% identity in 1069 aa overlap); P48633|HMP2_YEREN|IRP2 high-molecular-weight protein 2 (may be involved in the nonribosomal synthesis of small peptides) from Yersinia enterocolitica (2035 aa), FASTA scores: opt: 2275, E(): 9.4e-122, (38.45% identity in 1069 aa overlap); O85739|PCHE|PA4226 dihydroaeruginoic acid synthetase from Pseudomonas aeruginosa (1438 aa) FASTA scores: opt: 2236, E(): 1.2e-119, (38.2% identity in 1330 aa overlap); Q9RFM8|PCHE pyochelin synthetase from Pseudomonas aeruginosa (1438 aa), FASTA scores: opt: 2229,E(): 3e-119, (38.0% identity in 1329 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family.; Phenyloxazoline synthase MbtB (phenyloxazoline synthetase) 2675837 mbtB 885838 mbtB Mycobacterium tuberculosis H37Rv Phenyloxazoline synthase MbtB (phenyloxazoline synthetase) NP_216899.1 2671593 R 83332 CDS NP_216900.1 15609521 885833 2675936..2677633 1 NC_000962.3 Rv2384, (MTCY22H8.01, MTCY253.37c), len: 565 aa. mbtA, bifunctional enzyme, including salicyl-AMP ligase (Sal-AMP ligase) and salicyl-S-ArCP synthetase (see Quadri et al., 1998; De Voss et al., 1999), highly similar to other ligases e.g. Q9F638|MXCE from Stigmatella aurantiaca 2,3-DHBA-AMP ligase (protein involved in the biosynthesis of 2,3-dihydroxybenzoic acid, contains the AMP binding signature) (543 aa), FASTA scores: opt: 1683, E(): 2.8e-90,(48.25% identity in 545 aa overlap) (see Silakowski et al.,2000); P40871|DHBE_BACSU|ENTE 2,3-dihydroxybenzoate-AMP ligase from Bacillus subtilis (539 aa), FASTA scores: opt: 1569, E(): 1.2e-83, (44.9% identity in 532 aa overlap); O07899|VIBE_VIBCHVC0772 vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase from Vibrio cholerae (543 aa), FASTA scores: opt: 1457, E(): 3.7e-77, (44.6% identity in 545 aa overlap); etc. Also similar to P95819|SNBA pristinamycin I synthetase I from Streptomyces pristinaespiralis (582 aa), FASTA scores: opt: 1532, E(): 1.7e-81, (46.35% identity in 548 aa overlap); and Q9RFM9|PCHD salicyl-AMP ligase from Pseudomonas aeruginosa (547 aa), FASTA scores: opt: 1415, E(): 1e-74, (45.95% identity in 533 aa overlap). Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ArCP synthetase 2677633 mbtA 885833 mbtA Mycobacterium tuberculosis H37Rv Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ArCP synthetase NP_216900.1 2675936 D 83332 CDS YP_177876.1 57116980 885927 2677729..2678649 1 NC_000962.3 Rv2385, (MTCY253.36c), len: 306 aa. Putative mbtJ,acetyl hydrolase (see citations below), showing some similarity with various hydrolases including acetyl hydrolases e.g. Q9ZBM4|MLCB1450.08|ML0314 putative hydrolase/esterase from Mycobacterium leprae (335 aa),FASTA scores: opt: 449, E(): 6.7e-21, (33.85% identity in 313 aa overlap); AAK47950|MT3591 Esterase from M. tuberculosis strain CDC1551 (327 aa), FASTA scores: opt: 469, E(): 3.6e-22, (35% identity in 283 aa overlap); Q9X8J4|SCE9.22 putative esterase from Streptomyces coelicolor (266 aa), FASTA scores: opt: 430,E(): 8.5e-20,(38% identity in 245 aa overlap); Q01109|BAH_STRHY acetyl-hydrolase from Streptomyces hygroscopicus (299 aa),FASTA scores: opt: 420, E(): 4e-19, (35.1% identity in 265 aa overlap). Equivalent to AAK46748 from Mycobacterium tuberculosis strain CDC1551 (327 aa) but shorter 21 aa. Note that previously known as lipK.; Putative acetyl hydrolase MbtJ 2678649 mbtJ 885927 mbtJ Mycobacterium tuberculosis H37Rv Putative acetyl hydrolase MbtJ YP_177876.1 2677729 D 83332 CDS YP_177877.1 57116981 885823 complement(2678653..2680005) 1 NC_000962.3 Rv2386c, (MTCY253.35), len: 450 aa. mbtI,isochorismate synthase (see citations below), similar to Q9X9I8|IRP9 salicylate synthetase from Yersinia enterocolitica (434 aa), FASTA scores: opt: 887, E(): 7.5e-48, (37.45% identity in 422 aa overlap); and similar in C-terminal region to many anthranilate synthases component I e.g. Q9Z4W7|TRPE_STRCO|SCE8.07c from Streptomyces coelicolor (511 aa), FASTA scores: opt: 509,E(): 3e-24, (40.4% identity in 255 aa overlap); P33975|TRPE_HALVO from Halobacterium volcanii (Haloferax volcanii) (523 aa) FASTA scores: opt: 488, E(): 6.2e-23,(34.2% identity in 298 aa overlap); and similar to Q08653|TRPE_THEMA|TM0142 anthranilate synthase component I from Thermotoga maritima (461 aa), FASTA scores: opt: 478,E(): 2.3e-22, (28.4% identity in 440 aa overlap); etc. Could be belong to the anthranilate synthase component I family. Note that previously known as trpE2, an anthranilate synthase component I.; Isochorismate synthase MbtI 2680005 mbtI 885823 mbtI Mycobacterium tuberculosis H37Rv Isochorismate synthase MbtI YP_177877.1 2678653 R 83332 CDS NP_216903.1 15609524 885302 2680765..2682018 1 NC_000962.3 Rv2387, (MTCY253.34c), len: 417 aa. Conserved protein, showing some similarities with others e.g. Q9K663|BH3869 hypothetical protein from Bacillus halodurans (337 aa), FASTA scores: opt: 343, E(): 4.8e-14, (29.0% identity in 400 aa overlap); AAK25471|CC3509 hypothetical protein from Caulobacter crescentus (365 aa), FASTA scores: opt: 282, E(): 3.2e-10, (32.6% identity in 399 aa overlap); P73953|SLR1512 [D90911_21] conserved hypothetical protein from Synechocystis sp. strain PCC6803 (374 aa), FASTA scores: opt: 230, E(): 5.5e-07; (24.75% identity in 408 aa overlap); etc. Contains PS00213 Lipocalin signature.; hypothetical protein 2682018 885302 Rv2387 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216903.1 2680765 D 83332 CDS NP_216904.1 15609525 885300 complement(2682015..2683142) 1 NC_000962.3 Rv2388c, (MTCY253.33), len: 375 aa. Probable hemN,oxygen-independent coproporphyrinogen III oxidases, highly similar to many putative oxygen-independent coproporphyrinogen III oxidases e.g. Q9RDD2|SCC77.26 from Streptomyces coelicolor (435 aa), FASTA scores: opt: 1358,E(): 1.5e-76, (56.55% identity in 382 aa overlap); BAB51237|MLR4627 from Rhizobium loti (Mesorhizobium loti) (392 aa), FASTA scores: opt: 696, E(): 1.1e-35, (36.8% identity in 383 aa overlap); Q9KUR0|VC0455 from Vibrio cholerae (391 aa), FASTA scores: opt: 691, 2.2e-35, (32.65% identity in 386 aa overlap); P54304|HEMN_BACSU from Bacillus subtilis (366 aa), FASTA scores: opt: 668 , E(): 5.6e-34; (34.9% identity in 327 aa overlap); etc. Equivalent to AAK46752 from Mycobacterium tuberculosis strain CDC1551 (390 aa) but shorter 375 aa. Belongs to the anaerobic coproporphyrinogen III oxidase family.; Probable oxygen-independent coproporphyrinogen III oxidase HemN (coproporphyrinogenase) (coprogen oxidase) 2683142 hemN 885300 hemN Mycobacterium tuberculosis H37Rv Probable oxygen-independent coproporphyrinogen III oxidase HemN (coproporphyrinogenase) (coprogen oxidase) NP_216904.1 2682015 R 83332 CDS NP_216905.1 15609526 885246 complement(2683248..2683712) 1 NC_000962.3 Rv2389c, (MTCY253.32), len: 154 aa. Probable rpfD,resuscitation-promoting factor. Possible autocrine and/or paracrine bacterial growth factor or cytokine (see citation below). Similar to others from Mycobacterium tuberculosis e.g. O07747|Rv1884c|MTCY180.34|RPFC probable resuscitation-promoting factor from Mycobacterium tuberculosis (176 aa), FASTA scores: opt: 382, E(): 2.3e-17, (55.45% identity in 101 aa overlap); etc. Also similarity with Q9CBF8|ML2030 hypothetical protein from Mycobacterium leprae (157 aa), FASTA scores: opt: 397, E(): 2.4e-18, (47.95% identity in 121 aa overlap); Q9F2Q2|SCE41.06c putative secreted protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 341,E(): 1.1e-14, (40.45% identity in 131 aa overlap); and O86308|Z96935|MLRPF_1 RPF protein precursor from Micrococcus luteus (220 aa), FASTA scores: opt: 301, E(): 3.6e-12, (39.4% identity in 132 aa overlap). Contains a secretory signal sequence in N-terminus. Supposed acts at very low concentration. Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable resuscitation-promoting factor RpfD 2683712 rpfD 885246 rpfD Mycobacterium tuberculosis H37Rv Probable resuscitation-promoting factor RpfD NP_216905.1 2683248 R 83332 CDS NP_216906.1 15609527 885866 complement(2683709..2684266) 1 NC_000962.3 Rv2390c, (MTCY253.31), len: 185 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis proteins Q11032|YD62_MYCTU|MTCY02B10.26c|Rv1362c hypothetical 23.5 kDa protein (220 aa), FASTA scores: opt: 223, E(): 2.1e-07,(27.4% identity in 190 aa overlap); and Q11033|YD63_MYCTU|MTCY02B10.27c|Rv1363c hypothetical 28.3 kDa protein (261 aa), FASTA scores: opt: 238, E(): 2.7e-08,(27.6% identity in 163 aa overlap).; hypothetical protein 2684266 885866 Rv2390c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216906.1 2683709 R 83332 CDS NP_216907.1 15609528 885472 2684679..2686370 1 NC_000962.3 Rv2391, (MTCY253.30c), len: 563 aa. SirA,ferredoxin-dependent sulfite reductase (See Schnell et al.,2005). Previously annotated as nirA. Similar to e.g. CAC33947|SCBAC1A6.26c Putative nitrite/sulphite reductase from Streptomyces coelicolor (565 aa), FASTA scores: opt: 2335, E(): 1.2e-137, (60.1% identity in 567 aa overlap); Q9RZD6|DRA0013 ferredoxin-nitrite reductase from Deinococcus radiodurans (563 aa), FASTA scores: opt: 1141,E(): 2.2e-63, (39.6% identity in 533 aa overlap); Q59656|NIRA (D31732|PEENIRNRT_1) ferredoxin-dependent nitrite reductase from Plectonema boryanum (654 aa) (see Suzuki & Kikuchi 1995), FASTA scores: opt: 805, E(): 1.9e-42, (31.7% identity in 517 aa overlap); Q55366|NIRA|SLR0898 ferredoxin-nitrite reductase from Synechocystis sp. strain PCC 6803 (502 aa), FASTA scores: opt: 799, E(): 3.7e-42, (32.3% identity in 517 aa overlap); etc. Highly similar (only in N-terminal part because shortened protein (fragment) owing to an IS900 insertion) to Q9K541|NIRA nitrate reductase (fragment) from Mycobacterium paratuberculosis (198 aa), FASTA scores: opt: 798, E(): 2.1e-42, (65.4% identity in 182 aa overlap) (see Bull et al., 2000).; Ferredoxin-dependent sulfite reductase SirA 2686370 sirA 885472 sirA Mycobacterium tuberculosis H37Rv Ferredoxin-dependent sulfite reductase SirA NP_216907.1 2684679 D 83332 CDS NP_216908.1 15609529 885250 2686367..2687131 1 NC_000962.3 Rv2392, (MTCY253.29c), len: 254 aa. Probable cysH,3'-phosphoadenosine 5'-phosphosulfate reductase (see citation below), similar to many e.g. P94498|O34620|CYH1_BACSU|CYSH from Bacillus subtilis (233 aa), FASTA scores: opt: 618, E(): 8.1e-32, (46.5% identity in 202 aa overlap); Q9KCT3|CYSH|BH1486 from Bacillus halodurans (231 aa), FASTA scores: opt: 560, E(): 3.6e-28,(41.3% identity in 230 aa overlap); P56860|CYSH_DEIRA from Deinococcus radiodurans (255 aa), FASTA scores: opt: 489,E(): 1.1e-23, (44.7% identity in 190 aa overlap); etc. Belongs to the PAPS reductase family and CYSH subfamily. Note that operon cysA-cysW-cysT-subI, probably involved in sulfate transport, is near this putative ORF.; Probable 3'-phosphoadenosine 5'-phosphosulfate reductase CysH (PAPS reductase, thioredoxin DEP.) (padops reductase) (3'-phosphoadenylylsulfate reductase) (PAPS sulfotransferase) 2687131 cysH 885250 cysH Mycobacterium tuberculosis H37Rv Probable 3'-phosphoadenosine 5'-phosphosulfate reductase CysH (PAPS reductase, thioredoxin DEP.) (padops reductase) (3'-phosphoadenylylsulfate reductase) (PAPS sulfotransferase) NP_216908.1 2686367 D 83332 CDS NP_216909.1 15609530 885508 2687128..2687973 1 NC_000962.3 Rv2393, (MTCY253.28c), len: 281 aa. Che1,ferrochelatase (See Pinto et al., 2007). Conserved protein,with some similarity to Q9L2E8|SC7A8.10c putative secreted protein from Streptomyces coelicolor (274 aa), FASTA scores: opt: 407, E(): 2.8e-18, (37% identity in 246 aa overlap); CAC38793|SCI39.05 Conserved hypothetical protein from Streptomyces coelicolor (305 aa), FASTA scores: opt: 394, E(): 2e-17, (35.0% identity in 251 aa overlap); AAK44492|MT0272 Chalcone/stilbene synthase family protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 350, E(): 9.2e-15, (34.0% identity in 235 aa overlap); P95216|Rv0259c|MTCY06A4.03c|Z86089 hypothetical protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 345, E(): 1.9e-14,(33.6% identity in 235 aa overlap).; Ferrochelatase Che1 2687973 che1 885508 che1 Mycobacterium tuberculosis H37Rv Ferrochelatase Che1 NP_216909.1 2687128 D 83332 CDS NP_216910.1 15609531 885867 2688010..2689941 1 NC_000962.3 Rv2394, (MTCY253.27c), len: 643 aa. Probable ggtB,gamma-glutamyltranspeptidase precursor, similar to many e.g. Q9KVF2|VC0194 from Vibrio cholerae (588 aa), FASTA scores: opt: 943, E(): 7.5e-47, (40.0% identity in 597 aa overlap); O69935|SC3C8.26 from Streptomyces coelicolor (603 aa), FASTA scores: opt: 822, E(): 7.2e-40, (33.6% identity in 622 aa overlap); P54422|GGT_BACSU from Bacillus subtilis (587 aa) FASTA scores: opt: 491, E(): 8.2e-21, (33.4% identity in 574 aa overlap); etc. Has potential signal peptide and appropriately positioned prokaryotic lipoprotein attachment site (PS00013).; Probable gamma-glutamyltranspeptidase precursor GgtB (gamma-glutamyltransferase) (glutamyl transpeptidase) 2689941 ggtB 885867 ggtB Mycobacterium tuberculosis H37Rv Probable gamma-glutamyltranspeptidase precursor GgtB (gamma-glutamyltransferase) (glutamyl transpeptidase) NP_216910.1 2688010 D 83332 CDS NP_216911.1 15609532 885303 2690072..2692075 1 NC_000962.3 Rv2395, (MTCY253.26c), len: 667 aa. Probable conserved integral membrane protein, similar to AAK24613|CC2646 oligopeptide transporter/opt family protein from Caulobacter crescentus (666 aa), FASTA scores: opt: 1638, E(): 4.8e-86, (51.0% identity in 658 aa overlap); Q9PIS5|CJ0204 putative integral membrane protein from Campylobacter jejuni (665 aa), FASTA scores: opt: 1484,E(): 2.9e-77, (40.6% identity in 658 aa overlap); and P44016|Y561_HAEIN hypothetical integral membrane protein from Haemophilus influenzae (635 aa), FASTA scores: opt: 1449, E(): 2.8e-75, (42.15% identity in 624 aa overlap).; Probable conserved integral membrane protein 2692075 885303 Rv2395 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216911.1 2690072 D 83332 CDS YP_007411091.1 448817218 14515893 2692224..2692439 1 NC_000962.3 Rv2395A, len: 71 aa. AprA, acid and phagosome regulated protein A, restricted to M. tuberculosis complex. Note completely overlapped by sRNA mcr7.; Acid and phagosome regulated protein A AprA 2692439 aprA 14515893 aprA Mycobacterium tuberculosis H37Rv Acid and phagosome regulated protein A AprA YP_007411091.1 2692224 D 83332 CDS YP_007411092.1 448817219 14515879 2692551..2692715 1 NC_000962.3 Rv2395B, len: 54 aa. AprB, acid and phagosome regulated protein B, restricted to M. tuberculosis complex.; Acid and phagosome regulated protein B AprB 2692715 aprB 14515879 aprB Mycobacterium tuberculosis H37Rv Acid and phagosome regulated protein B AprB YP_007411092.1 2692551 D 83332 CDS YP_177878.1 57116982 885517 2692799..2693884 1 NC_000962.3 Rv2396, (MTCY253.25c), len: 361 aa. PE_PGRS41,member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Also known as aprC, acid and phagosome regulated protein C,restricted to M. tuberculosis complex (See Abramovitch et al., 2011). Contains PS00583 pfkB family of carbohydrate kinases signature 1. Predicted to be an outer membrane protein (See Song et al., 2008).; PE-PGRS family protein PE_PGRS41 2693884 PE_PGRS41 885517 PE_PGRS41 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS41 YP_177878.1 2692799 D 83332 CDS YP_177879.1 57116983 885663 complement(2693909..2694964) 1 NC_000962.3 Rv2397c, (MTCY253.24), len: 351 aa. cysA1,sulfate-transport ATP-binding protein ABC transporter (see citations below), similar to other sulfate ABC transporter ATP-binding proteins e.g. P14788|CYSA_SYNP7 from Synechococcus sp. (344 aa), FASTA scores: opt: 1112, E(): 2.6e-56, (54.6% identity in 328 aa overlap); P74548|CYSA_SYNY3 from Synechocystis sp. (355 aa), FASTA scores: opt: 1063, E(): 1.7e-53, (51.9% identity in 343 aa overlap); Q9I6L0|CYSA|PA0280 from Pseudomonas aeruginosa (329 aa), FASTA scores: opt: 987, E(): 3.3e-49, (49.2% identity in 339 aa overlap); etc. Also similar to many ATP-binding proteins from Mycobacterium tuberculosis e.g. Rv2038c, Rv1238, Rv2832c, etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Note that previously known as cysA.; Sulfate-transport ATP-binding protein ABC transporter CysA1 2694964 cysA1 885663 cysA1 Mycobacterium tuberculosis H37Rv Sulfate-transport ATP-binding protein ABC transporter CysA1 YP_177879.1 2693909 R 83332 CDS NP_216914.1 15609535 885305 complement(2694981..2695799) 1 NC_000962.3 Rv2398c, (MTCY253.23), len: 272 aa. Probable cysW,sulfate-transport integral membrane protein ABC transporter (see citations below), similar to others e.g. Q9K877|CYSW|BH3129 sulfate ABC transporter (permease) from Bacillus halodurans (287 aa), FASTA scores: opt: 765, E(): 4.1e-40, (43.8% identity in 249 aa overlap); P27370|CYSW_SYNP7 sulfate transport system (permease) protein from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (286 aa), FASTA scores: opt: 757, E(): 1.3e-39, (44.3% identity in 264 aa overlap); Q9I6K9|CYSW|PA0281 sulfate transport protein from Pseudomonas aeruginosa (289 aa), FASTA scores: opt: 753,E(): 2.3e-39, (44.4% identity in 250 aa overlap); P16702|P76534|CYSW_ECOLI sulfate transport system permease from Escherichia coli (291 aa), FASTA scores: opt: 633,E(): 5.7e-32, (38.2% identity in 267 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature. Similarity with integral membrane components of other binding-protein-dependent transport systems and belongs to the CYSTW subfamily.; Probable sulfate-transport integral membrane protein ABC transporter CysW 2695799 cysW 885305 cysW Mycobacterium tuberculosis H37Rv Probable sulfate-transport integral membrane protein ABC transporter CysW NP_216914.1 2694981 R 83332 CDS NP_216915.1 15609536 885301 complement(2695796..2696647) 1 NC_000962.3 Rv2399c, (MTCY253.22), len: 283 aa. Probable cysT,sulfate-transport integral membrane protein ABC transporter (see citations below), similar to others e.g. BAB48989|MLR1667 permease protein of sulfate ABC transporter from Rhizobium loti (283 aa), FASTA scores: opt: 756, E(): 7.9e-40, (40.95% identity in 271 aa overlap); Q9K878|cyst|BH3128 sulfate ABC transporter (permease) from Bacillus halodurans (279 aa), FASTA scores: opt: 750, E(): 1.8e-39, (44.55% identity in 258 aa overlap); P16701|CYST_ECOLI|CYSU|cyst|B2424 from Escherichia coli (277 aa), FASTA scores: opt: 669, E(): 1.9e-34, (40.0% identity in 260 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CYSTW subfamily.; Probable sulfate-transport integral membrane protein ABC transporter CysT 2696647 cysT 885301 cysT Mycobacterium tuberculosis H37Rv Probable sulfate-transport integral membrane protein ABC transporter CysT NP_216915.1 2695796 R 83332 CDS NP_216916.1 15609537 885299 complement(2696644..2697714) 1 NC_000962.3 Rv2400c, (MTCY253.21), len: 356 aa. Probable subI,sulfate-binding lipoprotein component of sulfate transport system (see citations below), equivalent to Q9CCN3|SUBI|ML0615 (alias Q49748|B1937_F1_11, 358 aa) putative sulphate-binding protein from Mycobacterium leprae (348 aa), FASTA scores: opt: 1775, E(): 2.3e-102, (76.45% identity in 340 aa overlap). Also similar to others and other substrate-binding proteins e.g. P27366|SUBI_SYNP7|SBPA sulfate-binding protein precursor from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (350 aa), FASTA scores: opt: 703, E(): 4.6e-36, (35.6% identity in 351 aa overlap); Q9I6K7|SBP|PA0283 sulfate-binding protein precursor from Pseudomonas aeruginosa (332 aa), FASTA scores: opt: 591, E(): 3.7e-29,(36.9% identity in 317 aa overlap); CAC49112|SMB21133 putative sulfate uptake ABC transporter periplasmic solute-binding protein precursor from Rhizobium meliloti (Sinorhizobium meliloti) (341 aa), FASTA scores: opt: 569,E(): 8.8e-28, (36.15% identity in 321 aa overlap); etc. Belongs to the prokaryotic sulfate binding protein family.; Probable sulfate-binding lipoprotein SubI 2697714 subI 885299 subI Mycobacterium tuberculosis H37Rv Probable sulfate-binding lipoprotein SubI NP_216916.1 2696644 R 83332 CDS NP_216917.2 57116984 885664 2697728..2698057 1 NC_000962.3 Rv2401, (MTCY253.19c), len: 109 aa. Hypothetical unknown protein. Equivalent to AAK46768 from Mycobacterium tuberculosis strain CDC1551 (134 aa) but shorter 25 aa. N-terminus extended since first submission (previously 72 aa).; Hypothetical protein 2698057 885664 Rv2401 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216917.2 2697728 D 83332 CDS YP_177670.1 57116985 3205070 complement(2698042..2698245) 1 NC_000962.3 Rv2401A, len: 67 aa. Possible conserved membrane protein, highly similar, but with 29 aa shorter, to ML0614|AL583919_34|Q49760 from Mycobacterium leprae (95 aa), FASTA scores: opt: 297, E(): 3.6e-15, (67.7% identity in 65 aa overlap). Has hydrophobic stretch.; Possible conserved membrane protein 2698245 3205070 Rv2401A Mycobacterium tuberculosis H37Rv Possible conserved membrane protein YP_177670.1 2698042 R 83332 CDS NP_216918.3 448824785 885661 2698529..2700457 1 NC_000962.3 Rv2402, (MTCY253.18c), len: 642 aa. Conserved protein, highly similar to others e.g. 9X8C4|SCE36.11c conserved hypothetical protein (fragment) from Streptomyces coelicolor (612 aa), FASTA scores: opt: 1283, E(): 6.5e-75,(41.9% identity in 623 aa overlap); Q9RJ38|SCI8.15 hypothetical 66.3 KDA protein from Streptomyces coelicolor (595 aa), FASTA scores: opt: 1152, E(): 1.7e-66, (39.9% identity in 622 aa overlap), Q9S223|CI51.17 hypothetical 68.4 KDA protein from Streptomyces coelicolor (612 aa),FASTA scores: opt: 1146, E(): 4.2e-66, (40.6% identity in 623 aa overlap); YAY3_SCHPO|Q10211|c4h3.03c hypothetical 74.5 kDa protein from Schizosaccharomyces pombe (Fission yeast) (649 aa) FASTA scores: opt: 999, E(): 1.3e-56,(35.0% identity in 642 aa overlap); etc. Contains possible helix-turn-helix motif, at aa 224-245 (+4.68 SD).; hypothetical protein 2700457 885661 Rv2402 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216918.3 2698529 D 83332 CDS NP_216919.1 15609540 885513 complement(2700535..2701290) 1 NC_000962.3 Rv2403c, (MTCY253.17), len: 251 aa. Probable lppR,conserved lipoprotein, with weak similarity with mycobacterial serine/threonine protein kinases e.g. AAK45563|MT1304 from Mycobacterium tuberculosis strain CDC1551 (626 aa), FASTA scores: opt: 186, E(): 0.00023,(24.4% identity in 238 aa overlap), and the C-terminal part of Q11053|Rv1266c|MTCY50.16|PKNH_MYCTU from Mycobacterium tuberculosis (626 aa), FASTA scores: opt: 185, E()= 0.00027, (24.35% identity in 238 aa overlap). Has signal peptide and appropriate positioned prokaryotic lipoprotein attachment site (PS00013). Could belong to the Ser/Thr family of protein kinases.; Probable conserved lipoprotein LppR 2701290 lppR 885513 lppR Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppR NP_216919.1 2700535 R 83332 CDS NP_216920.1 15609541 885475 complement(2701287..2703248) 1 NC_000962.3 Rv2404c, (MT2476, MTCY253.16), len: 653 aa. Probable lepA, GTP-binding protein (a protein of unknown function,but apparently with membrane-related functions and very similar to protein synthesis elongation factors; see citations below). Equivalent to P53530|LEPA_MYCLE|ML0611|B1937_F3_81 GTP-binding protein from Mycobacterium leprae (646 aa), FASTA scores: opt: 3610, E(): 1.2e-205, (88.0% identity in 649 aa overlap). Also highly similar to many GTP-binding proteins LEPA e.g. Q9RDC9|LEPA_STRCO|SCC77.29c from Streptomyces coelicolor (622 aa), FASTA scores: opt: 3046, E(): 2.3e-172, (74.3% identity in 626 aa overlap); P37949|LEPA_BACSU from B. subtilis (612 aa), FASTA scores: opt: 2430, E(): 5.3e-136,(58.7% identity in 610 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, LEPA subfamily.; Probable GTP-binding protein LepA (GTP-binding elongation factor) 2703248 lepA 885475 lepA Mycobacterium tuberculosis H37Rv Probable GTP-binding protein LepA (GTP-binding elongation factor) NP_216920.1 2701287 R 83332 CDS NP_216921.1 15609542 885507 2703269..2703838 1 NC_000962.3 Rv2405, (MTCY253.15c), len: 189 aa. Conserved protein, identical (but N-terminus longer 40 residues) to AAK46773|MT2477 hypothetical protein from Mycobacterium tuberculosis strain CDC1551. Also highly similar, but N-terminus longer 38 residues, to Q9RD03|SCCM1.41 hypothetical 17.4 KDA protein from Streptomyces coelicolor (154 aa), FASTA scores: opt: 451, E(): 2e-22, (48.7% identity in 154 aa overlap). Shows also similarity with hypothetical proteins from other species.; hypothetical protein 2703838 885507 Rv2405 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216921.1 2703269 D 83332 CDS NP_216922.1 15609543 885156 complement(2704009..2704437) 1 NC_000962.3 Rv2406c, (MTCY253.14), len: 142 aa. Conserved protein. C-terminal region is identical with many CBS domain protein e.g. AAK46774|MT2478 CBS domain protein from Mycobacterium tuberculosis strain CDC1551 (aa 47-142),FASTA scores: opt: 594, E(): 1.9e-30, (98.97% identity in 97 aa overlap); etc. Also similar to other hypothetical proteins e.g. AAK24594|CC2626 CBS domain protein from Caulobacter crescentus (157 aa), FASTA scores: opt: 377,E(): 8.3e-17, (42.55% identity in 141 aa overlap); BAB47826|MLR0188 from Rhizobium loti; etc.; hypothetical protein 2704437 885156 Rv2406c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216922.1 2704009 R 83332 CDS NP_216923.1 15609544 885684 2704697..2705518 1 NC_000962.3 Rv2407, (MTCY253.13c), len: 273 aa. Conserved hypothetical protein, highly similar (but longer at N-terminus) to AAK46775|MT2479 putative arylsulfatase from Mycobacterium tuberculosis strain CDC1551 (224 aa) FASTA scores: opt: 1433, E(): 2.5e-81, (96.43% identity in 224 aa overlap); O33130|MLCL536.01 hypothetical protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 658, E(): 1.5e-33, (56.75% identity in 215 aa overlap). Also similar to AAK23160|CC1176 Metallo-beta-lactamase family protein from Caulobacter crescentus (317 aa), FASTA scores: opt: 286, E(): 1.8e-10, (33% identity in 291 aa overlap). And similar to other hypothetical proteins eg Q49744|B1937_C1_163 hypothetical 22.6 KDA protein (precursor) from Mycobacterium leprae (211 aa), FASTA scores: opt: 623, E(): 2.1e-31, (56.3% identity in 206 aa overlap); O27859|MTH1831 conserved protein from Methanothermobacter thermautotrophicus (307 aa), FASTA scores: opt: 268, E(): 2.3e-09, (28.35% identity in 307 aa overlap); etc.; hypothetical protein 2705518 885684 Rv2407 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216923.1 2704697 D 83332 CDS YP_177880.1 57116986 885511 2706017..2706736 1 NC_000962.3 Rv2408, (MTCY253.12c), len: 239 aa. Possibly PE24, a member of PE family (see citation below), similar to AAK46440|MT2159 from Mycobacterium tuberculosis strain CDC1551 (491 aa) FASTA scores: opt: 269, E(): 5.4e-08,(38.45% identity in 156 aa overlap) and AAK45466|MT1209 from Mycobacterium tuberculosis strain CDC1551 (308 aa),FASTA scores: opt: 265, E(): 6.3e-08, (36.0% identity in 197 aa overlap).; Possible PE family-related protein PE24 2706736 PE24 885511 PE24 Mycobacterium tuberculosis H37Rv Possible PE family-related protein PE24 YP_177880.1 2706017 D 83332 CDS NP_216925.1 15609546 885674 complement(2706494..2707333) 1 NC_000962.3 Rv2409c, (MTCY253.11), len: 279 aa. Conserved protein, equivalent to Q49757|YP69_MYCLE|G466976|B1937_F2_39 hypothetical protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 1564, E(): 4.6e-95, (82.1% identity in 279 aa overlap). Also similar to others e.g. Q9RSX6|DR1993 from Deinococcus radiodurans (274 aa), FASTA scores: opt: 494, E(): 4e-25,(35.1% identity in 282 aa overlap); BAB49898|Mll2875 from Rhizobium loti (Mesorhizobium loti) (294 aa), FASTA scores: opt: 382, E(): 8.9e-18, (29.75% identity in 269 aa overlap); Q9I305|PA1732 from Pseudomonas aeruginosa (266 aa), FASTA scores: opt: 326, E(): 3.7e-14, (31.25% identity in 275 aa overlap); etc. Also similar to Rv2569c|MTCY227.32 from Mycobacterium tuberculosis.; hypothetical protein 2707333 885674 Rv2409c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216925.1 2706494 R 83332 CDS NP_216926.1 15609547 885257 complement(2707333..2708310) 1 NC_000962.3 Rv2410c, (MTCY253.10), len: 325 aa. Conserved protein, equivalent to Q49770|CAC30114|ML0606 conserved hypothetical protein from Mycobacterium leprae (325 aa),FASTA scores: opt: 1928, E(): 3.5e-117, (90.75% identity in 325 aa overlap). Also some similarity with other hypothetical proteins e.g. Q9RST2|DR2041 conserved hypothetical protein from Deinococcus radiodurans (316 aa),FASTA scores: opt: 329, E(): 5.3e-14, (32.4% identity in 318 aa overlap); C-terminus of Q9HUN7|PA4927 hypothetical protein from Pseudomonas aeruginosa (830 aa), FASTA scores: opt: 297, E(): 1.5e-11, (27.6% identity in 315 aa overlap); etc.; hypothetical protein 2708310 885257 Rv2410c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216926.1 2707333 R 83332 CDS NP_216927.1 15609548 885681 complement(2708310..2709965) 1 NC_000962.3 Rv2411c, (MTCY253.09c), len: 551 aa. Hypothetical protein, highly similar to Q49755|YO11_MYCLE|ML0605|MLCL536.05c|U1937B|B1937_F1_4 hypothetical 61.8 KDA protein from Mycobacterium leprae (561 aa), FASTA scores, opt: 3163, E(): 4.1e-178, (87.35% identity in 554 aa overlap). Also highly similar, except in N-terminus, to others e.g. Q55587|Y335_SYNY3|SLL0335 hypothetical protein from Synechocystis sp. strain PCC 6803 (481 aa), FASTA scores: opt: 1620, E(): 1.2e-87, (52.8% identity in 468 aa overlap); Q9I307|PA1730 hypothetical protein from Pseudomonas aeruginosa (470 aa), FASTA scores: opt: 1574, E(): 5.8e-85, (52.7% identity in 467 aa overlap); Q9RST1|DR2042 conserved hypothetical protein from Deinococcus radiodurans (655 aa), FASTA scores: opt: 1561,E(): 4.4e-84, (53.3% identity in 467 aa overlap); etc.; hypothetical protein 2709965 885681 Rv2411c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216927.1 2708310 R 83332 CDS NP_216928.1 15609549 885676 2710075..2710335 1 NC_000962.3 Rv2412, (MT2485, MTCY253.08c), len: 86 aa. rpsT, 30s ribosomal protein s20, equivalent to O33132|RS20_MYCLE|L0604|MLCL536.06 30S ribosomal protein S20 from Mycobacterium leprae (86 aa), FASTA scores: opt: 456, E(): 4.6e-24, (87.20% identity in 86 aa overlap). Also highly similar or similar to others e.g. Q9RDM3|RPST|SCC123.01 30S ribosomal protein S20 from Streptomyces coelicolor (88 aa), FASTA scores: opt: 363,E(): 7.1e-18, (70.95% identity in 86 aa overlap); Q9KD79|RPST|BH1339 ribosomal protein S20 (BS20) from Bacillus halodurans (91 aa), FASTA scores: opt: 252, E(): 1.8e-10, (49.4% identity in 85 aa overlap); P02378|RS20_ECOLI 30s ribosomal protein s20 from Escherichia coli (86 aa), FASTA scores: opt: 210, E(): 1e-07, (42.4% identity in 85 aa overlap); etc. Belongs to the S20P family of ribosomal proteins.; 30S ribosomal protein S20 RpsT 2710335 rpsT 885676 rpsT Mycobacterium tuberculosis H37Rv 30S ribosomal protein S20 RpsT NP_216928.1 2710075 D 83332 CDS NP_216929.3 448824786 885666 complement(2710351..2711301) 1 NC_000962.3 Rv2413c, (MTCY253.07), len: 316 aa. Conserved hypothetical protein, highly similar to O33133|MLCL536.07c|ML0603|Q49756|G466975|B1937_F2_36 hypothetical 39.1 KDA protein from Mycobacterium leprae (389 aa), FASTA scores: opt: 1683, E(): 1.8e-88, (83.9% identity in 316 aa overlap). ML0603 is a putative lipoprotein with an N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site that is not present in Rv2413c as this seems to be 73 aa shorter. Also some similarity with various proteins from other organisms e.g. Q9RDM2|SCC123.02c putative DNA-binding protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 792,E(): 6.1e-38, (42.4% identity in 316 aa overlap); Q9HX31|HOLA|PA3989 DNA polymerase III, delta subunit from Pseudomonas aeruginosa (345 aa), FASTA scores: opt: 173,E(): 0.0084, (25.4% identity in 307 aa overlap); etc.; hypothetical protein 2711301 885666 Rv2413c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216929.3 2710351 R 83332 CDS NP_216930.1 15609551 885667 complement(2711332..2712876) 1 NC_000962.3 Rv2414c, (MTCY253.06), len: 514 aa. Conserved hypothetical protein, showing some similarity with come operon proteins 3 (COMEC or COME3) e.g. Q9RTB1|DR1854 putative competence protein COMEC/REC2 from Deinococcus radiodurans (755 aa), FASTA scores: opt: 311, E(): 8.2e-11,(27.3% identity in 538 aa overlap); P73100|come|SLL1929 come protein from Synechocystis sp. strain PCC 6803 (709 aa), FASTA scores: opt: 302, E(): 2.6e-10, (26.3% identity in 323 aa overlap) (no similarity on N-terminus); P39695|CME3_BACSU come operon protein 3 from Bacillus subtilis (776 aa), FASTA scores: opt: 273, E(): 1.4e-08,(25.2% identity in 282 aa overlap) (no similarity on N-terminus); etc.; hypothetical protein 2712876 885667 Rv2414c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216930.1 2711332 R 83332 CDS NP_216931.1 15609552 885697 complement(2712891..2713784) 1 NC_000962.3 Rv2415c, (MTCY253.05), len: 297 aa. Hypothetical protein, with some similarity in C-terminal part to comE operon proteins 1 e.g. Q9EU10|come|COME4|COME1|COME2|COME3 come protein (a competence protein with DNA-binding activity) from Neisseria gonorrhoeae (99 aa), FASTA scores: opt: 190, E(): 0.0032, (49.2% identity in 61 aa overlap); Q9JYB8|NMB1657 from Neisseria meningitidis (205 aa) FASTA scores: opt: 191, E(): 0.0052, (49.2% identity in 61 aa overlap); CME1_BACSU|P39694 come operon protein 1 from Bacillus subtilis (205 aa), FASTA scores, opt: 181, E(): 0.017 (29.8% identity in 218 aa overlap); etc.; hypothetical protein 2713784 885697 Rv2415c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216931.1 2712891 R 83332 CDS NP_216932.2 57116987 885903 complement(2714124..2715332) 1 NC_000962.3 Rv2416c, (MTCY253.04), len: 402 aa. Eis, enhanced intracellular survival gene (see citations below). Conserved hypothetical protein, contains GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part, similar to Q9F309|SCC80.10 hypothetical 44.7 KDA protein from Streptomyces coelicolor (413 aa), FASTA scores: opt: 382,E(): 1e-16, (31.45% identity in 407 aa overlap); Q9K4F4|SCD66.23 conserved hypothetical protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 238,E(): 1.3e-07, (36.5% identity in 364 aa overlap): and Q54238|G1139577|ORF5 hypothetical protein from Streptomyces griseus (416 aa), FASTA scores: opt: 237, E(): 1.5e-07,(34.0 identity in 423 aa overlap). Start changed since first submission (- 6 aa) (see Dahl et al., 2001; Wei et al., 2000; Vetting et al. 2005).; Enhanced intracellular survival protein Eis,GCN5-related N-acetyltransferase 2715332 eis 885903 eis Mycobacterium tuberculosis H37Rv Enhanced intracellular survival protein Eis,GCN5-related N-acetyltransferase NP_216932.2 2714124 R 83332 CDS NP_216933.1 15609554 885692 complement(2715472..2716314) 1 NC_000962.3 Rv2417c, (MTCY253.03), len: 280 aa. Conserved protein, highly similar to Q9RDL7|SCC123.07c hypothetical 29.2 KDA protein from Streptomyces coelicolor (281 aa),FASTA scores: opt: 579, E(): 3.6e-27, (38.3% identity in 274 aa overlap). Also some similarity with DEGV proteins or hypothetical proteins from other organisms, e.g. Q9RSY3|DR1986 from Deinococcus radiodurans (281 aa), FASTA scores: opt: 393, E(): 3.4e-16, (31.0% identity in 280 aa overlap); P32436|DEGV_BACSU from Bacillus subtilis (281 aa), FASTA scores: opt: 365, E(): 1.5e-14, (27.8% identity in 284 aa overlap); BAB41937|BAB46307|SA0704|SAV0749 Conserved hypothetical protein from Staphylococcus aureus strain Mu50 and N315 (288 aa), FASTA scores: opt: 371, E(): 7e-15, (28.85% identity in 281 aa overlap); etc.; hypothetical protein 2716314 885692 Rv2417c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216933.1 2715472 R 83332 CDS NP_216934.1 15609555 885304 complement(2716395..2717138) 1 NC_000962.3 Rv2418c, (MTCY253.02), len: 247 aa. Unknown protein.; hypothetical protein 2717138 885304 Rv2418c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216934.1 2716395 R 83332 CDS NP_216935.1 15609556 885727 complement(2717128..2717799) 1 NC_000962.3 Rv2419c, (MTCY428.28-MTCY253.01), len: 223 aa. gpgP,glucosyl-3-phosphoglycerate phosphatase (See Mendes et al.,2011). Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature. Belongs to the phosphoglycerate mutase family. Enzyme activity inhibited by Co2+ and Cu2+ (See Mendes et al., 2011).; Glucosyl-3-phosphoglycerate phosphatase GpgP 2717799 gpgP 885727 gpgP Mycobacterium tuberculosis H37Rv Glucosyl-3-phosphoglycerate phosphatase GpgP NP_216935.1 2717128 R 83332 CDS NP_216936.1 15609557 885677 complement(2717796..2718176) 1 NC_000962.3 Rv2420c, (MTCY428.27), len: 126 aa. Conserved hypothetical protein, equivalent to Q9CBZ9|ML1453 hypothetical protein from Mycobacterium leprae (129 aa),FASTA scores: opt: 681, E(): 1.6e-38, (87.0% identity in 123 aa overlap). Also highly similar to Q9RDK9|SCC123.15c hypothetical protein from Streptomyces coelicolor (148 aa),FASTA scores: opt: 447, E(): 5.8e-23, (52.7% identity in 129 aa overlap); and similar to others e.g. P54457|YQEL_BACSU hypothetical protein from Bacillus subtilis (118 aa), FASTA scores: opt: 318, E(): 1.8e-14,(37.3% identity in 110 aa overlap); Q9KD89|BH1328 hypothetical protein from Bacillus halodurans (117 aa),FASTA scores: opt: 296, E(): 5.1e-13, (37.6% identity in 109 aa overlap); etc.; hypothetical protein 2718176 885677 Rv2420c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216936.1 2717796 R 83332 CDS NP_216937.1 15609558 885457 complement(2718173..2718808) 1 NC_000962.3 Rv2421c, (MT2494, MTCY428.26), len: 211 aa. Probable nadD, nicotinate-nucleotide adenylyltransferase ,equivalent to Q9CBZ8|NADD_MYCLE|ML1454 probable nicotinate-nucleotide adenylyltransferase from Mycobacterium leprae (214 aa), FASTA scores: opt: 1125,E(): 2.7e-66, (80.2% identity in 212 aa overlap). Also highly similar to Q9RDK7|NADD_STRCO probable nicotinate-nucleotide adenylyltransferase from Streptomyces coelicolor (188 aa), FASTA scores: opt: 855, E(): 9.8e-49,(66.5% identity in 194 aa overlap); and similar to others e.g. P54455|NADD_BACSU from Bacillus subtilis (189 aa),FASTA scores: opt: 351, E(): 7e-16, (36.1% identity in 191 aa overlap); etc. Belongs to the NadD family.; Probable nicotinate-nucleotide adenylyltransferase NadD (deamido-NAD(+) pyrophosphorylase) (deamido-NAD(+) diphosphorylase) (nicotinate mononucleotide adenylyltransferase) (NAMN adenylyltransferase) 2718808 nadD 885457 nadD Mycobacterium tuberculosis H37Rv Probable nicotinate-nucleotide adenylyltransferase NadD (deamido-NAD(+) pyrophosphorylase) (deamido-NAD(+) diphosphorylase) (nicotinate mononucleotide adenylyltransferase) (NAMN adenylyltransferase) NP_216937.1 2718173 R 83332 CDS NP_216938.1 15609559 885531 2719083..2719355 1 NC_000962.3 Rv2422, (MTCY428.25c), len: 90 aa. Hypothetical unknown protein.; Hypothetical protein 2719355 885531 Rv2422 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216938.1 2719083 D 83332 CDS NP_216939.1 15609560 885516 2719597..2720643 1 NC_000962.3 Rv2423, (MTCY428.24c), len: 348 aa. Hypothetical unknown protein.; Hypothetical protein 2720643 885516 Rv2423 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216939.1 2719597 D 83332 CDS NP_216940.1 15609561 885699 complement(2720776..2721777) 1 NC_000962.3 Rv2424c, (MTCY428.23), len: 333 aa. Probable transposase for IS1558, similar to is element proteins e.g. AL021957|Rv2177c|MTV021_10 from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 1491, E(): 6.2e-87, (98.6% identity in 221 aa overlap); P19780|YIS1_STRCO hypothetical insertion element IS110 from Streptomyces coelicolor (45 aa), FASTA scores: opt: 203, E(): 1.7e-05; (27.3% identity in 238 aa overlap); etc. Contains PS01159 WW/rsp5/WWP domain signature.; Probable transposase 2721777 885699 Rv2424c Mycobacterium tuberculosis H37Rv Probable transposase NP_216940.1 2720776 R 83332 CDS NP_216941.1 15609562 885673 complement(2721866..2723308) 1 NC_000962.3 Rv2425c, (MTCY428.22), len: 480 aa. Hypothetical protein; C-terminal half shares similarity to other unknown conserved proteins e.g. Q53065 hypothetical 24.3 KDA protein from Rhodococcus erythropolis (219 aa), FASTA scores: opt: 398, E(): 9.9e-17, (34.15% identity in 202 aa overlap); C-terminus of O27843|MTH1815 conserved protein from Methanothermobacter thermautotrophicus (346 aa), FASTA scores: opt: 341, E(): 3.7e-13, (31.35% identity in 233 aa overlap); etc.; hypothetical protein 2723308 885673 Rv2425c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216941.1 2721866 R 83332 CDS NP_216942.1 15609563 885909 complement(2723308..2724183) 1 NC_000962.3 Rv2426c, (MTCY428.21), len: 291 aa. Conserved hypothetical protein, highly similar to others e.g. Q51326|ORF4 from Pseudomonas carboxydovorans (295 aa),FASTA scores: opt: 853, E(): 3.7e-43, (48.75% identity in 277 aa overlap); BAB47746|MLR0088 from Rhizobium loti (309 aa), FASTA scores: opt :809, E(): 1.5e-40, (46.5% identity in 291 aa overlap); Q9Y9R8|APE2220 from Aeropyrum pernix (297 aa), FASTA scores: opt: 763, E(): 7.4e-38, (47.1% identity in 261 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; hypothetical protein 2724183 885909 Rv2426c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216942.1 2723308 R 83332 CDS NP_216943.1 15609564 885536 complement(2724230..2725477) 1 NC_000962.3 Rv2427c, (MTCY428.20), len: 415 aa. Probable proA,gamma-glutamyl phosphate reductase protein, equivalent to Q9CBZ7|ML1458|PROA [gamma]-glutamyl phosphate reductase from Mycobacterium leprae (409 aa), FASTA scores: opt: 2120, E(): 7.4e-118, (81.9% identity in 409 aa overlap). Also highly similar or similar to other gamma-glutamyl phosphate reductases proteins (GPR) e.g. Q9RDK1|PROA from Streptomyces coelicolor (428 aa), FASTA scores: opt: 1073,E(): 4.6e-56, (60.4% identity in 429 aa overlap); P45638|PROA_CORGL from Corynebacterium glutamicum (432 aa),FASTA scores: opt: 993, E(): 2.4e-51, (58.5% identity in 417 aa overlap); P96489|PROA_STRTR gamma-glutamyl phosphate reductase from Streptococcus thermophilus (416 aa), FASTA scores: opt: 863, E(): 1.1e-43, (49.15% identity in 413 aa overlap); etc. Belongs to the gamma-glutamyl phosphate reductase family.; Probable gamma-glutamyl phosphate reductase protein ProA (GPR) (glutamate-5-semialdehyde dehydrogenase) (glutamyl-gamma-semialdehyde dehydrogenase) 2725477 proA 885536 proA Mycobacterium tuberculosis H37Rv Probable gamma-glutamyl phosphate reductase protein ProA (GPR) (glutamate-5-semialdehyde dehydrogenase) (glutamyl-gamma-semialdehyde dehydrogenase) NP_216943.1 2724230 R 83332 CDS NP_216944.1 15609565 885717 2726193..2726780 1 NC_000962.3 Rv2428, (MTCY428.18c), len: 195 aa. AhpC, alkyl hydroperoxide reductase C (see citations below), equivalent to other alkyl hydroperoxide reductases C mycobacterial proteins e.g. Q9CBF5|AHPC|ML2042 alkyl hydroperoxide reductase from Mycobacterium leprae (195 aa) FASTA scores: opt: 1183, E(): 2.6e-72, (88.20% identity in 195 aa overlap); O87323|AHPC from Mycobacterium marinum (195 aa),FASTA scores: opt: 1215, E(): 1.9e-74, (90.8% identity in 195 aa overlap); Q57413|AHPC|AVI-3 from Mycobacterium avium (195 aa), FASTA scores: opt: 1201, E(): 1.6e-73, (90.25% identity in 195 aa overlap). Also highly similar to others from other organisms e.g. Q9FBP5|AHPC alkyl hydroperoxide reductase from Streptomyces coelicolor (184 aa), FASTA scores: opt: 768, E(): 1.7e-44, (62.45% identity in 189 aa overlap); etc.; Alkyl hydroperoxide reductase C protein AhpC (alkyl hydroperoxidase C) 2726780 ahpC 885717 ahpC Mycobacterium tuberculosis H37Rv Alkyl hydroperoxide reductase C protein AhpC (alkyl hydroperoxidase C) NP_216944.1 2726193 D 83332 CDS NP_216945.1 15609566 885959 2726806..2727339 1 NC_000962.3 Rv2429, (MTCY428.17c), len: 177 aa. AhpD, alkyl hydroperoxide reductase, similar to other alkyl hydroperoxide reductases D proteins e.g. Q9RN73|AHPD from Streptomyces coelicolor (178 aa), FASTA scores: opt: 611,E(): 1.4e-33, (57.4% identity in 169 aa overlap); Q50441|AHPD_MYCSM AHPD protein (fragment) from Mycobacterium smegmatis (52 aa), FASTA score: opt:196.; Alkyl hydroperoxide reductase D protein AhpD (alkyl hydroperoxidase D) 2727339 ahpD 885959 ahpD Mycobacterium tuberculosis H37Rv Alkyl hydroperoxide reductase D protein AhpD (alkyl hydroperoxidase D) NP_216945.1 2726806 D 83332 CDS YP_177881.1 57116988 885945 complement(2727336..2727920) 1 NC_000962.3 Rv2430c, (MTCY428.16), len: 194 aa. PPE41, Member of the Mycobacterium tuberculosis PPE family similar to others e.g. AAK46014|Rv1745|MT1745 from Mycobacterium tuberculosis (385 aa) FASTA scores: opt: 389, E(): 1.2e-17, (35.95% identity in 192 aa overlap); etc.; PPE family protein PPE41 2727920 PPE41 885945 PPE41 Mycobacterium tuberculosis H37Rv PPE family protein PPE41 YP_177881.1 2727336 R 83332 CDS YP_177882.1 57116989 885703 complement(2727967..2728266) 1 NC_000962.3 Rv2431c, (MTCY428.15), len: 99 aa. PE25, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to others e.g. AAK47158|MT2839 from Mycobacterium tuberculosis (275 aa) FASTA scores: opt: 194,E(): 2.5e-06, (40.0% identity in 95 aa overlap); etc.; PE family protein PE25 2728266 PE25 885703 PE25 Mycobacterium tuberculosis H37Rv PE family protein PE25 YP_177882.1 2727967 R 83332 CDS NP_216948.1 15609569 885075 complement(2728437..2728847) 1 NC_000962.3 Rv2432c, (MTCY428.14), len: 136 aa. Hypothetical unknown protein.; Hypothetical protein 2728847 885075 Rv2432c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216948.1 2728437 R 83332 CDS NP_216949.1 15609570 885579 complement(2728844..2729134) 1 NC_000962.3 Rv2433c, (MTCY428.13), len: 96 aa. Hypothetical unknown protein.; Hypothetical protein 2729134 885579 Rv2433c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216949.1 2728844 R 83332 CDS NP_216950.1 15609571 885885 complement(2729115..2730560) 1 NC_000962.3 Rv2434c, (MTCY428.12), len: 481 aa. Probable conserved transmembrane protein, with some similarity to BAB48444|MLR0973 probable integral membrane protein from Rhizobium loti (410 aa), FASTA scores: opt: 298, E(): 4.1e-11, (27.25% identity in 389 aa overlap); and also similarity with other hypothetical proteins and/or putative integral membrane proteins.; Probable conserved transmembrane protein 2730560 885885 Rv2434c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_216950.1 2729115 R 83332 CDS NP_216951.1 15609572 885891 complement(2730557..2732749) 1 NC_000962.3 Rv2435c, (MTCY428.11), len: 730 aa. Probable cyclase (adenylyl- or guanylyl-cyclase; EC 4.6.1.1 or 4.6.1.2 respectively); C-terminal domain (aa 500-730) similar to domain at C-terminus of a series of adenylate/guanylate cyclases e.g. O30820|CYA AAK45931|MT1661 from Mycobacterium tuberculosis (443 aa) FASTA scores: opt: 446, E(): 1.3e-19,(30.55% identity in 301 aa overlap); BAB50179|MLL3242 cyclase (adenylyl or guanylyl) from Rhizobium loti (356 aa), FASTA scores: opt: 372, E(): 3.4e-15, (28.75% identity in 219 aa overlap); etc. Belongs to adenylyl cyclase class-4/guanylyl cyclase family.; Probable cyclase (adenylyl-or guanylyl-)(adenylate-or guanylate-) 2732749 885891 Rv2435c Mycobacterium tuberculosis H37Rv Probable cyclase (adenylyl-or guanylyl-)(adenylate-or guanylate-) NP_216951.1 2730557 R 83332 CDS NP_216952.1 15609573 885671 2733230..2734144 1 NC_000962.3 Rv2436, (MTCY428.10c), len: 304 aa. Probable rbsK,ribokinase, similar to others e.g. Q9RZ99|DRA0055 from Deinococcus radiodurans (300 aa) FASTA scores: opt: 485,E(): 9.1e-21, (44.55% identity in 301 aa overlap); P36945|P96733|RBSK_BACSU from Bacillus subtilis (293 aa),FASTA scores: opt: 398, E(): 8.5e-16, (36.35% identity in 297 aa overlap); P05054|RBSK_ECOLI|B3752|Z5253|ECS4694 from Escherichia coli strain K12 (309 aa), FASTA scores: opt: 387, E(): 3.8e-15, (34.7% identity in 314 aa overlap); etc. Contains PS00583 pfkB family of carbohydrate kinases signature 1. Belongs to the PFKB family of carbohydrate kinases.; Ribokinase RbsK 2734144 rbsK 885671 rbsK Mycobacterium tuberculosis H37Rv Ribokinase RbsK NP_216952.1 2733230 D 83332 CDS NP_216953.1 15609574 885906 2734376..2734795 1 NC_000962.3 Rv2437, (MTCY428.09c), len: 139 aa. Conserved transmembrane protein, with some similarity to conserved hypothetical proteins e.g. O06539|RV1139C|MTCI65.06c from Mycobacterium tuberculosis (166 aa); AAK45430|MT1172 from Mycobacterium tuberculosis (124 aa), FASTA scores: opt: 166, E(): 0.00013, (35.7% identity in 112 aa overlap); BAB48937|Mlr1600 from Rhizobium loti (222 aa), FASTA scores: opt: 163 ,E(): 0.00033, (28.1% identity in 121 aa overlap); etc. Contains membrane spanning regions.; Conserved transmembrane protein 2734795 885906 Rv2437 Mycobacterium tuberculosis H37Rv Conserved transmembrane protein NP_216953.1 2734376 D 83332 CDS NP_216954.2 57116990 885808 complement(2734792..2736831) 1 NC_000962.3 Rv2438c, (MT2513, MTCY428.08), len: 679 aa. NadE,glutamine-dependent NAD(+) synthetase (see citation below),equivalent to Q9CBZ6|NADE_MYCLE|ML1463 Glutamine-dependent NAD(+) synthetase from Mycobacterium leprae (680 aa), FASTA scores: opt: 3877, E(): 0. Also similar to others e.g. O83759|NADE_TREPA|TP0780 from Treponema pallidum (679 aa),FASTA scores: opt: 543, E(): 1.1e-25; O74940|NADE_SCHPO|SPCC553.02 from Schizosaccharomyces pombe (Fission yeast) (700 aa), FASTA scores: opt: 354, E(): 4.7e-14 ; P38795|NADE_YEAST|YHR074W from Saccharomyces cerevisiae (Baker's yeast) (714 aa), FASTA scores: opt: 339, E(): 4e-13; etc. Contains PS00591 Glycosyl hydrolases family 10 active site. Belongs to the NAD synthetase family in the C-terminal section. N-terminus shorter since first submission.; Glutamine-dependent NAD(+) synthetase NadE (NAD(+) synthase [glutamine-hydrolysing]) 2736831 nadE 885808 nadE Mycobacterium tuberculosis H37Rv Glutamine-dependent NAD(+) synthetase NadE (NAD(+) synthase [glutamine-hydrolysing]) NP_216954.2 2734792 R 83332 CDS YP_177671.1 57116991 3205071 2736709..2736987 1 NC_000962.3 Rv2438A, len: 92 aa. Conserved hypothetical protein,showing few similarity with various enzymes e.g. part of O83441|VAA1_TREPA|ATPA1|TP0426 V-type ATP synthase alpha chain 1 from Treponema pallidum (589 aa), FASTA scores: opt: 110, E(): 1.5, (40.3% identity in 72 aa overlap); N-terminus of O95178|NIGM_HUMAN NADH-ubiquinone oxidoreductase AGGG subunit precursor from Homo sapiens (105 aa), FASTA scores: opt: 109, E(): 1.5, (35.5% identity in 62 aa overlap); N-terminus of Q9HJ76|TA1096 probable glycerol kinase from Thermoplasma acidophilum (488 aa); etc.; hypothetical protein 2736987 3205071 Rv2438A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177671.1 2736709 D 83332 CDS NP_216955.1 15609576 885266 complement(2737117..2738247) 1 NC_000962.3 Rv2439c, (MTCY428.07), len: 376 aa. Probable proB,glutamate 5-kinase protein (GK), equivalent to Q9CBZ5|prob|ML1464 from Mycobacterium leprae (367 aa) FASTA scores: opt: 1937, E(): 1.1e-102, (84.4% identity in 366 aa overlap). Also highly similar to other glutamate 5-kinase proteins e.g. P46546|PROB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (369 aa), FASTA scores: opt: 1241, E(): 3e-63, (54.35% identity in 368 aa overlap); Q9ZG98|PROB_MEIRU glutamate 5-kinase from Meiothermus ruber (390 aa), FASTA scores: opt: 825, E(): 1.2e-39, (45.05% identity in 353 aa overlap); Q9RDJ9|prob|SCC123.25c from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1193,E(): 1.6e-60, (55.85% identity in 367 aa overlap); etc. Contains PS00902 Glutamate 5-kinase signature. Belongs to the glutamate 5-kinase family.; Probable glutamate 5-kinase protein ProB (gamma-glutamyl kinase) (GK) 2738247 proB 885266 proB Mycobacterium tuberculosis H37Rv Probable glutamate 5-kinase protein ProB (gamma-glutamyl kinase) (GK) NP_216955.1 2737117 R 83332 CDS NP_216956.1 15609577 885900 complement(2738247..2739686) 1 NC_000962.3 Rv2440c, (MTCY428.06), len: 479 aa. Probable obg,nucleotide-binding protein, equivalent to Q9CBZ4|ML1465 GTP1/OBG-family GTP-binding protein from Mycobacterium leprae (478 aa), FASTA scores: opt: 1328, E(): 8.4e-70,(58.9% identity in 479 aa overlap). Also highly similar to others e.g. P95722|OBG GTP-binding protein from Streptomyces coelicolor (478 aa), FASTA scores: opt: 1311,E(): 8.2e-69, (60.7% identity in 476 aa overlap); P20964|OBG_BACSU SPO0B-associated GTP-binding protein from Bacillus subtilis (428 aa), FASTA scores: opt: 1006, E(): 3.9e-51, (42.9% identity in 436 aa overlap); Q9KDK0|OBG|BH1213 GTP-binding protein involved in initiation of sporulation from Bacillus halodurans (427 aa), FASTA scores: opt: 978, E(): 1.7e-49, (41.95% identity in 436 aa overlap); etc. Highly similar (identical but shorter 5 aa) to AAK46813|MT2516 GTP-binding protein from Mycobacterium tuberculosis strain CDC1551 (484 aa), FASTA scores: opt: 3205, E(): 7.9e-179, (100% identity in 479 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the GTP1/OBG family.; Probable GTP1/Obg-family GTP-binding protein Obg 2739686 obg 885900 obg Mycobacterium tuberculosis H37Rv Probable GTP1/Obg-family GTP-binding protein Obg NP_216956.1 2738247 R 83332 CDS NP_216957.1 15609578 885919 complement(2739772..2740032) 1 NC_000962.3 Rv2441c, (MTCY428.05), len: 86 aa. rpmA, 50S ribosomal proteins L27, equivalent to Q9CBZ3|RL27_MYCLE from Mycobacterium leprae (88 aa), FASTA scores: opt: 504,E(): 7.6e-28, (93.2% identity in 81 aa overlap). Also highly similar to others e.g. P95757|RL27_STRGR from Streptomyces griseus (85 aa), FASTA scores: opt: 442, E(): 1.2e-23, (81.5% identity in 81 aa overlap); etc. Contains PS00831 Ribosomal protein L27 signature. Belongs to the L27P family of ribosomal proteins.; 50S ribosomal protein L27 RpmA 2740032 rpmA 885919 rpmA Mycobacterium tuberculosis H37Rv 50S ribosomal protein L27 RpmA NP_216957.1 2739772 R 83332 CDS NP_216958.1 15609579 885715 complement(2740047..2740361) 1 NC_000962.3 Rv2442c, (MTCY428.04), len: 104 aa. rplU, 50S ribosomal protein L21, equivalent to Q9CBZ2|RL21_MYCLE from Mycobacterium leprae (103 aa), FASTA scores: opt: 579, E(): 4.8e-31, (91.1% identity in 102 aa overlap). Also highly similar to others e.g. P95756|RL21_STRGR from Streptomyces griseus (106 aa), FASTA scores: opt: 362, E(): 5.4e-17,(56.0% identity in 100 aa overlap); etc.; 50S ribosomal protein L21 RplU 2740361 rplU 885715 rplU Mycobacterium tuberculosis H37Rv 50S ribosomal protein L21 RplU NP_216958.1 2740047 R 83332 CDS NP_216959.1 15609580 885745 2740709..2742184 1 NC_000962.3 Rv2443, (MTCY428.03c), len: 491 aa. Probable dctA,C4-dicarboxylate-transport transmembrane protein, similar to other C4-dicarboxylate transport proteins e.g. AAK46817|MT2519 from Mycobacterium tuberculosis strain CDC1551 (491 aa); Q9L1K8|SC6A11.12 putative sodium:dicarboxylate symporter from Streptomyces coelicolor (466 aa), FASTA scores: opt: 1797, E(): 2.9e-98, (61.3% identity in 452 aa overlap); Q9RRG7|DR2525 from Deinococcus radiodurans (463 aa); P50334|DCTA_SALTY from Salmonella typhimurium (428 aa) FASTA scores: opt: 1241, E(): 1.3e-65,(47.2% identity in 415 aa overlap); etc. Belongs to the sodium dicarboxylate symporter family (SDF) (DAACS family).; Probable C4-dicarboxylate-transport transmembrane protein DctA 2742184 dctA 885745 dctA Mycobacterium tuberculosis H37Rv Probable C4-dicarboxylate-transport transmembrane protein DctA NP_216959.1 2740709 D 83332 CDS NP_216960.1 15609581 885911 complement(2742123..2744984) 1 NC_000962.3 Rv2444c, (MTCY428.02), len: 953 aa. Possible rne,ribonuclease E, highly similar to others e.g. Q9CBZ1|ML1468 possible ribonuclease from Mycobacterium leprae (924 aa),FASTA scores: opt: 3713, E(): 2.4e-174, (74.2% identity in 966 aa overlap); Q9SI08|AT2G04270 putative ribonuclease E from Arabidopsis thaliana (502 aa), FASTA scores: opt: 674,E(): 7.5e-26, (31.2% identity in 410 aa overlap); etc. Similar at C-terminal end to P21513|RNE_ECOLI|ams|HMP1|B1084 ribonuclease E (RNASE E) from Escherichia coli strain K12 (1061 aa), FASTA scores: opt: 554, E(): 9.9e-20, (37.8% identity in 386 aa overlap). Also similar in medium part to several cytoplasmic axial filament proteins e.g. Q9HVU4|CAFA|PA4477 from Pseudomonas aeruginosa (485 aa), FASTA scores: opt: 664, E(): 2.3e-25,(42.8% identity in 418 aa overlap); etc. Equivalent to AAK46818 from Mycobacterium tuberculosis strain CDC1551 (621 aa) but longer 332 aa in N-terminal part. Seems to belong to the RNE family.; Possible ribonuclease E Rne 2744984 rne 885911 rne Mycobacterium tuberculosis H37Rv Possible ribonuclease E Rne NP_216960.1 2742123 R 83332 CDS NP_216961.1 15609582 885905 complement(2745314..2745724) 1 NC_000962.3 Rv2445c, (MTV008.01c, MTCY428.01), len: 136 aa. Probable ndkA (alternate gene name: ndk), nucleoside diphosphate kinase, equivalent to Q9CBZ0|NDK|ML1469 from Mycobacterium leprae (136 aa), FASTA scores: opt: 762, E(): 1.5e-42, (87.4% identity in 135 aa overlap); and O85501|NDK from Mycobacterium smegmatis (139 aa), FASTA scores: opt: 714, E(): 1.9e-39, (80.7% identity in 135 aa overlap). Also highly similar to others e.g. P50589|NDK_STRCO from Streptomyces coelicolor (137 aa), FASTA scores: opt: 535,6.8e-28, (60.3% identity in 136 aa overlap); O29491|NDK_ARCFU|AF0767 from Archaeoglobus fulgidus (151 aa), FASTA scores: opt: 521, E(): 5.9e-27, (58.0% identity in 131 aa overlap); P31103|NDK_BACSU from Bacillus subtilis (151 aa), FASTA scores: opt: 515, E(): 1.4e-26, (56.5% identity in 131 aa overlap); etc. Belongs to the NDK family. Ppk2|Rv3232c and NdkA|Rv2445c interact (See Sureka et al., 2009).; Probable nucleoside diphosphate kinase NdkA (NDK) (NDP kinase) (nucleoside-2-P kinase) 2745724 ndkA 885905 ndkA Mycobacterium tuberculosis H37Rv Probable nucleoside diphosphate kinase NdkA (NDK) (NDP kinase) (nucleoside-2-P kinase) NP_216961.1 2745314 R 83332 CDS NP_216962.1 15609583 885916 complement(2745767..2746138) 1 NC_000962.3 Rv2446c, (MTV008.02c), len: 123 aa. Probable conserved integral membrane protein, highly similar to Q9CBY9|ML1470 conserved membrane protein from Mycobacterium leprae (123 aa), FASTA scores: opt: 468, E(): 6.7e-23,(66.65% identity in 108 aa overlap). Also similar to Q9L1G5|SCC88.24c putative membrane protein from Streptomyces coelicolor (118 aa), FASTA scores: opt: 130,E(): 0.13, (37.2% identity in 86 aa overlap); and some similarity to O06852|Y13070 hypothetical Streptomyces coelicolor gene also between fpgs and ndk genes (see citation below) (117 aa), FASTA scores: opt: 128, E(): 0.17, (36.0% identity in 86 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved integral membrane protein 2746138 885916 Rv2446c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_216962.1 2745767 R 83332 CDS NP_216963.1 15609584 885902 complement(2746135..2747598) 1 NC_000962.3 Rv2447c, (MTV008.03c), len: 487 aa. Probable folC,folylpolyglutamate synthase, equivalent to Q9CBY8|FOLC|ML1471 from Mycobacterium leprae (485 aa),FASTA scores: opt: 2425, E(): 2.2e-134, (78.7% identity in 483 aa overlap). Also highly similar to others e.g. Q9L1G4|FPGS|O08416|Y13070 from Streptomyces coelicolor (444 aa), FASTA scores: opt: 774, E(): 6.3e-38, (53.9% identity in 462 aa overlap); P15925|FOLC_LACCA|FGS from Lactobacillus casei (428 aa), FASTA scores: opt: 631, E(): 1.4e-29, (34.55% identity in 437 aa overlap); Q05865|FOLC_BACSU from Bacillus subtilis (430 aa), FASTA scores: opt: 421, E(): 2.6e-17, (32.9% identity in 383 aa overlap); etc. Contains PS01012 Folylpolyglutamate synthase signature 2. Belongs to the folylpolyglutamate synthase family.; Probable folylpolyglutamate synthase protein FolC (folylpoly-gamma-glutamate synthetase) (FPGS) 2747598 folC 885902 folC Mycobacterium tuberculosis H37Rv Probable folylpolyglutamate synthase protein FolC (folylpoly-gamma-glutamate synthetase) (FPGS) NP_216963.1 2746135 R 83332 CDS NP_216964.1 15609585 885892 complement(2747595..2750225) 1 NC_000962.3 Rv2448c, (MTV008.04c), len: 876 aa. Probable valS,valyl-tRNA synthetases, equivalent to Q9CBY7|VALS|ML1472 valyl-tRNA synthase from Mycobacterium leprae (886 aa),FASTA scores: opt: 5181,E(): 0, (85.4% identity in 876 aa overlap). Also highly similar to others e.g. O06851|SYV_STRCO from Streptomyces coelicolor (874 aa),FASTA scores: opt: 2470, E(): 1.6e-143, (60.45% identity in 880 aa overlap); Q9X2D7|SYV_THEMA|VALS|TM1817 from Thermotoga maritima (865 aa), FASTA scores: opt: 2418, E(): 2.4e-140, (44.2% identity in 891 aa overlap); Q05873|SYV_BACSU|VALS from Bacillus subtilis (880 aa),FASTA scores: opt: 2063, E(): 1.4e-118, (46.08% identity in 894 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Contains probable coiled-coil from aa 810 to 846. Belongs to class-I aminoacyl-tRNA synthetase family.; Probable valyl-tRNA synthase protein ValS (valyl-tRNA synthetase) (valine--tRNA ligase) (valine translase) 2750225 valS 885892 valS Mycobacterium tuberculosis H37Rv Probable valyl-tRNA synthase protein ValS (valyl-tRNA synthetase) (valine--tRNA ligase) (valine translase) NP_216964.1 2747595 R 83332 CDS NP_216965.1 15609586 885894 complement(2750313..2751572) 1 NC_000962.3 Rv2449c, (MTV008.05c), len: 419 aa. Conserved protein, highly similar to hypothetical proteins e.g. P95139|Rv2953|MTCY349.37c from M. tuberculosis (418 aa),FASTA scores: opt: 1829, E(): 4.7e-103, (67.3% identity in 419 aa overlap); AAK47353|MT3027 from Mycobacterium tuberculosis strain CDC1551 (418 aa), FASTA score: opt: 1829, E(): 4.7e-103, (67.3 identity in 419 aa overlap); Q9CD87|ML0129 from Mycobacterium leprae (418 aa), FASTA scores: opt: 1727, E(): 6.8e-97, (65.45% identity in 414 aa overlap); etc.; hypothetical protein 2751572 885894 Rv2449c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216965.1 2750313 R 83332 CDS NP_216966.1 15609587 885760 complement(2751662..2752180) 1 NC_000962.3 Rv2450c, (MTV008.06c), len: 172 aa. Probable rpfE,resuscitation-promoting factor (see Mukamolova et al.,1998), similar to O86308|Z96935|MLRPF_1 RPF protein precursor from Micrococcus luteus (220 aa), FASTA scores: opt: 291, E(): 3e-7, (48.75% identity in 80 aa overlap). C-terminus is similar to other Mycobacterial rpf proteins e.g. O05594|Rv1009|MTCI237.26|RPFB probable resuscitation-promoting factor from Mycobacterium tuberculosis (362 aa), FASTA scores: opt: 344, E(): 1.4e-09, (42.85% identity in 147 aa overlap); etc. C-terminal region similar to N-terminal region of Q9F2Q2|SCE41.06c putative secreted protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 355,E(): 3.1e-10, (56.65% identity in 90 aa overlap). Also similar to Q9F2Q1|SCE41.07c putative secreted protein from Streptomyces coelicolor (near Q9F2Q2|SCE41.06c) (341 aa) FASTA scores: opt: 317, E(): 2.5e-08, (51.7% identity in 87 aa overlap). With Mycobacterium leprae, high similarity between the two corresponding C-terminal regions of two hypothetical proteins, Q9CD53|ML0240 (375 aa), FASTA scores: opt: 339, E(): 2.5e-09, (59.15% identity in 93 aa overlap) and O33049|MLCB57.05c|ML2151 (174 aa), FASTA scores: opt: 329, E(): 4e-09, (58.14% identity in 86 aa overlap). Contains a possible secretory signal sequence in N-terminus. Possible autocrine and/or paracrine bacterial growth factor or cytokine (see citations below). Interacts with RipA (see Hett et al., 2007). Predicted possible vaccine candidate (See Zvi et al., 2008).; Probable resuscitation-promoting factor RpfE 2752180 rpfE 885760 rpfE Mycobacterium tuberculosis H37Rv Probable resuscitation-promoting factor RpfE NP_216966.1 2751662 R 83332 CDS NP_216967.1 15609588 886025 2752262..2752660 1 NC_000962.3 Rv2451, (MTV008.07), len: 132 aa. Hypothetical unknown pro-, ser-rich protein.; Hypothetical proline and serine rich protein 2752660 886025 Rv2451 Mycobacterium tuberculosis H37Rv Hypothetical proline and serine rich protein NP_216967.1 2752262 D 83332 CDS NP_216968.1 15609589 885858 complement(2752848..2752994) 1 NC_000962.3 Rv2452c, (MTV008.08c), len: 48 aa. Hypothetical unknown protein (see citation below).; Hypothetical protein 2752994 885858 Rv2452c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216968.1 2752848 R 83332 CDS NP_216969.1 15609590 885904 complement(2753018..2753623) 1 NC_000962.3 Rv2453c, (MT2528, MTV008.09c), len: 201 aa. Probable mobA, molybdopterin-guanine dinucleotide biosynthesis protein A, similar to others e.g. Q9F8G7 from Carboxydothermus hydrogenoformans (224 aa), FASTA scores: opt: 249, E(): 3.9e-08, (30.6% identity in 173 aa overlap); P95645|MOBA_RHOSH|mob|Y09560 from Rhodobacter sphaeroides (199 aa), FASTA scores: opt: 240, E(): 1.2e-07, (33.9% identity in 186 aa overlap); Q9X7K0|MOBA_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (191 aa), FASTA scores: opt: 217, E(): 2.9e-06, (37.4% identity in 123 aa overlap); etc. Belongs to the MobA family.; Probable molybdopterin-guanine dinucleotide biosynthesis protein A MobA 2753623 mobA 885904 mobA Mycobacterium tuberculosis H37Rv Probable molybdopterin-guanine dinucleotide biosynthesis protein A MobA NP_216969.1 2753018 R 83332 CDS NP_216970.1 15609591 887435 complement(2753625..2754746) 1 NC_000962.3 Rv2454c, (MTV008.10c), len: 373 aa. Probable oxidoreductase, beta subunit, similar to Q9F2W7|SCD20.12c putative oxidoreductase from Streptomyces coelicolor (352 aa), FASTA scores: opt: 1461, E(): 6.4e-85, (65.3% identity in 343 aa overlap) alias Q9RKS5|STAH10.34c putative oxidoreductase beta-subunit from Streptomyces coelicolor (350 aa), FASTA scores: opt: 1429, E(): 6.7e-83, (64.0% identity in 342 aa overlap); and similar in part to others e.g. Q9Z5X3 ferredoxin oxidoreductase B-subunit from Frankia sp. (346 aa), FASTA scores: opt: 1143, E(): 7.5e-65, (51.2% identity in 336 aa overlap); BAB21495|KORB ferredoxin oxidoreductase beta subunit from Hydrogenobacter thermophilus TK-6 (295 aa), FASTA scores: opt: 682, E(): 8.3e-36, (48.25% identity in 201 aa overlap); etc. Note that the upstream ORF (MTV008.11c|Rv2455c) is possibly an oxidoreductase alpha subunit.; Probable oxidoreductase (beta subunit) 2754746 887435 Rv2454c Mycobacterium tuberculosis H37Rv Probable oxidoreductase (beta subunit) NP_216970.1 2753625 R 83332 CDS NP_216971.1 15609592 887370 complement(2754743..2756704) 1 NC_000962.3 Rv2455c, (MTV008.11c), len: 653 aa. Probable oxidoreductase, alpha subunit, similar to others e.g. Q9F2W6|SCD20.13c putative oxidoreductase from Streptomyces coelicolor (645 aa), FASTA scores: opt: 2017, E(): 1e-111,(66.45% identity in 617 aa overlap) alias Q9RKS4|STAH10.35c putative oxidoreductase alpha-subunit from Streptomyces coelicolor (630 aa), FASTA scores: opt: 2008, E(): 3.4e-111, (66.45% identity in 614 aa overlap); Q9YA13|APE2126 long hypothetical 2-oxoacid--ferredoxin oxidoreductase alpha chain from Aeropyrum pernix (644 aa) FASTA scores: opt: 687, E(): 4.6e-33, (33.35% identity in 441 aa overlap); etc. Note that the downstream ORF (MTV008.10c|Rv2454c) is possibly an oxidoreductase beta subunit.; Probable oxidoreductase (alpha subunit) 2756704 887370 Rv2455c Mycobacterium tuberculosis H37Rv Probable oxidoreductase (alpha subunit) NP_216971.1 2754743 R 83332 CDS NP_216972.1 15609593 887340 complement(2756936..2758192) 1 NC_000962.3 Rv2456c, (MTV008.12c), len: 418 aa. Probable conserved integral membrane transport protein, involved in a efflux system, weakly similar to many e.g. Q9RUR0|YD22_DEIRA|DR1322 putative sugar efflux transporter from Deinococcus radiodurans (389 aa), FASTA scores: opt: 224, E(): 8.4e-06, (24.45% identity in 409 aa overlap); Q9UYY0|PAB0913 multidrug resistance protein from Pyrococcus abyssi (410 aa), FASTA scores: opt: 210, E(): 5.6e-05,(21.8% identity in 408 aa overlap); etc. Contains PS00216 Sugar transport proteins signature 1.; Probable conserved integral membrane transport protein 2758192 887340 Rv2456c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein NP_216972.1 2756936 R 83332 CDS NP_216973.1 15609594 888167 complement(2758208..2759488) 1 NC_000962.3 Rv2457c, (MTV008.13c), len: 426 aa. Probable clpX,ATP-dependent clp protease ATP-binding subunit clpX,equivalent to Q9CBY6|CLPX|ML1477 ATP-dependent CLP protease ATP-binding protein from Mycobacterium leprae (426 aa),FASTA scores: opt: 2652, E(): 1.4e-142, (96.0% identity in 426 aa overlap). Also highly similar to others e.g. Q9F316|CLPX from Streptomyces coelicolor (428 aa) FASTA scores: opt: 2178, E(): 8.2e-116, (77.8% identity in 428 aa overlap); P50866|CLPX_BACSU from Bacillus subtilis (420 aa), FASTA scores: opt: 1788, E(): 8.5e-94, (63.6% identity in 426 aa overlap); P33138|CLPX_ECOLI from Escherichia coli (423 aa), FASTA scores: opt: 1694, E(): 1.7e-88, (62.4% identity in 415 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CLPX chaperone family. Conserved in M. tuberculosis, M. leprae,M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable ATP-dependent CLP protease ATP-binding subunit ClpX 2759488 clpX 888167 clpX Mycobacterium tuberculosis H37Rv Probable ATP-dependent CLP protease ATP-binding subunit ClpX NP_216973.1 2758208 R 83332 CDS NP_216974.1 15609595 885871 2759779..2760687 1 NC_000962.3 Rv2458, (MTV008.14), len: 302 aa. Probable mmuM,homocysteine S-methyltransferase, equivalent to Q9CBY5|ML1478 possible transferase from Mycobacterium leprae (293 aa), FASTA scores: opt: 1507, E(): 2.7e-86,(78.85% identity in 293 aa overlap). Also similar to others e.g. Q47690|MMUM_ECOLI|B0261 homocysteine S-methyltransferase from Escherichia coli strain K12 (310 aa), FASTA scores: opt: 863, E(): 2.4e-46, (47.65% identity in 298 aa overlap); Q9FUM7 homocysteine S-methyltransferase-4 from Zea mays (Maize) (342 aa), FASTA scores: opt: 324, E(): 6.8e-13, (44.45% identity in 306 aa overlap); Q9LUI7|HMT3 cysteine methyltransferase from Arabidopsis thaliana (Mouse-ear cress) (347 aa), FASTA scores: opt: 312, E(): 3.8e-12, (41.85% identity in 313 aa overlap); etc. Identical to AAK46833|MT2533 homocysteine S-methyltransferase from Mycobacterium tuberculosis strain CDC1551 (302 aa).; Probable homocysteine S-methyltransferase MmuM (S-methylmethionine:homocysteine methyltransferase) (cysteine methyltransferase) 2760687 mmuM 885871 mmuM Mycobacterium tuberculosis H37Rv Probable homocysteine S-methyltransferase MmuM (S-methylmethionine:homocysteine methyltransferase) (cysteine methyltransferase) NP_216974.1 2759779 D 83332 CDS NP_216975.1 15609596 888191 2760854..2762380 1 NC_000962.3 Rv2459, (MTV008.15), len: 508 aa. Probable conserved integral membrane transport protein, member of major facilitator superfamily (MFS) possibly involved in drug transport, highly similar to many efflux proteins e.g. Q9RL22|SC5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 788,E(): 1.3e-38, (34.45% identity in 412 aa overlap); Q9I428|PA1316 probable MFS transporter from Pseudomonas aeruginosa (513 aa), FASTA scores: opt: 782, E(): 3.1e-38,(32.75% identity in 519 aa overlap); P39886|TCMA_STRGA tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 752,E(): 1.8e-36, (31.7% identity in 511 aa overlap); etc. Also highly similar to AAK46687|MT2395 drug transporter from Mycobacterium tuberculosis strain CDC1551 (537 aa), FASTA scores: opt: 1396, E(): 5.6e-74, (44.45% identity in 504 aa overlap); and P71879|Rv2333c|MTCY3G12.01 probable conserved integral membrane transport protein from Mycobacterium tuberculosis strain H37Rv (537 aa), FASTA scores: opt: 1385, E(): 2.5e-73, (44.25% identity in 504 aa overlap).; Probable conserved integral membrane transport protein 2762380 888191 Rv2459 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein NP_216975.1 2760854 D 83332 CDS NP_216976.1 15609597 888174 complement(2762531..2763175) 1 NC_000962.3 Rv2460c, (MT2535, MTV008.16c), len: 214 aa. Probable clpP2, ATP-dependent clp protease proteolytic subunit 2,equivalent to Q9CBY4|CLP2_MYCLE ATP-dependent CLP protease proteolytic subunit from Mycobacterium leprae (214 aa). Also highly similar to others e.g. Q9ZH58|CLPP2 from Streptomyces coelicolor (236 aa), FASTA scores: opt: 918,E(): 2.1e-50, (66.35% identity in 214 aa overlap); O67357|CLPP_AQUAE|AQ_1339 from Aquifex aeolicus (201 aa),FASTA scores: opt: 680, E(): 1.4e-35, (52.0% identity in 194 aa overlap); P43867|CLPP_HAEIN from Haemophilus influenzae (193 aa), FASTA scores: opt: 662, E(): 1.8e-34,(53.35% identity in 193 aa overlap); etc. Contains PS00381 Endopeptidase Clp serine active site. Also similar to upstream ORF Rv2461c|MTV008.17c|clpP1 (200 aa), FASTA score: (48.3% identity in 172 aa overlap). Belongs to peptidase family S14, also known as ClpP family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable ATP-dependent CLP protease proteolytic subunit 2 ClpP2 (endopeptidase CLP 2) 2763175 clpP2 888174 clpP2 Mycobacterium tuberculosis H37Rv Probable ATP-dependent CLP protease proteolytic subunit 2 ClpP2 (endopeptidase CLP 2) NP_216976.1 2762531 R 83332 CDS YP_177883.1 57116992 888176 complement(2763172..2763774) 1 NC_000962.3 Rv2461c, (MT2536, MTV008.17c), len: 200 aa. Probable clpP1, ATP-dependent clp protease proteolytic subunit 1,equivalent to Q9CBY3|CLP1_MYCLE ATP-dependent CLP protease proteolytic subunit from Mycobacterium leprae (224 aa),FASTA scores: opt: 1226, E(): 1.3e-71, (95.0% identity in 200 aa overlap). Also highly similar to others e.g. Q9F315|CLPP1 from Streptomyces coelicolor (219 aa), FASTA scores: opt: 713, E(): 9.3e-39, (61.75% identity in 183 aa overlap); P80244|CLPP_BACSU from Bacillus subtilis (197 aa), FASTA scores: opt: 658, E(): 2.8e-35, (54% identity in 187 aa overlap); Q9WZF9|CLPP_THEMA|TM0695 from Thermotoga maritima (203 aa), FASTA scores: opt: 653, E(): 6.1e-35,(55.25% identity in 172 aa overlap); etc. Also similar to downstream ORF Rv2460c|MTV008.16c|clpP2 (214 aa), FASTA score: (48.3% identity in 172 aa overlap). Belongs to peptidase family S14, also known as CLPP family. Note that previously known as clp. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable ATP-dependent CLP protease proteolytic subunit 1 ClpP1 (endopeptidase CLP) 2763774 clpP1 888176 clpP1 Mycobacterium tuberculosis H37Rv Probable ATP-dependent CLP protease proteolytic subunit 1 ClpP1 (endopeptidase CLP) YP_177883.1 2763172 R 83332 CDS NP_216978.1 15609599 888615 complement(2763891..2765291) 1 NC_000962.3 Rv2462c, (MTV008.18c), len: 466 aa. Probable tig,trigger factor (TF), a chaperone protein, equivalent to Q9CBY2|ML1481 possible molecular chaperone from Mycobacterium leprae (469 aa), FASTA scores: opt: 2171,E(): 7.2e-113, (70.1% identity in 468 aa overlap). Also similar to oyher trigger factors from several organisms e.g. Q9F314|SCC80.05c from Streptomyces coelicolor (468 aa), FASTA scores: opt: 1224, E(): 1.7e-60, (41.8% identity in 469 aa overlap); Q9K8F3|TIG_BACHD from Bacillus halodurans (431 aa), FASTA scores: opt: 675, E(): 3.6e-30,(28.5% identity in 421 aa overlap); P22257|TIG_ECOLI from Escherichia coli (432 aa), FASTA scores: opt: 493, E(): 4.2e-20, (23.35% identity in 433 aa overlap); etc. Belongs to the FKBP-type PPIase family, TIG subfamily.; Probable trigger factor (TF) protein Tig 2765291 tig 888615 tig Mycobacterium tuberculosis H37Rv Probable trigger factor (TF) protein Tig NP_216978.1 2763891 R 83332 CDS NP_216979.1 15609600 888572 2765655..2766839 1 NC_000962.3 Rv2463, (MTV008.19), len: 394 aa. Probable lipP,esterase, lipase similar to others eg O87861|ESTA esterase a from Streptomyces chrysomallus (389 aa), FASTA scores: opt: 964, E(): 1.9e-53, (44.35% identity in 399 aa overlap); Q9I4S7|PA1047 probable esterase from Pseudomonas aeruginosa (392 aa), FASTA scores: opt: 863, E(): 4.6e-47,(40.05% identity in 377 aa overlap); Q53403|ESTC esterase III from Pseudomonas fluorescens (382 aa), FASTA scores: opt: 753, E(): 3.9e-40, (36.3% identity in 380 aa overlap); etc.; Probable esterase/lipase LipP 2766839 lipP 888572 lipP Mycobacterium tuberculosis H37Rv Probable esterase/lipase LipP NP_216979.1 2765655 D 83332 CDS NP_216980.1 15609601 888500 complement(2766859..2767665) 1 NC_000962.3 Rv2464c, (MT2539, MTV008.20c), len: 268 aa. Possible DNA glycosylase, showing some similarity to several other DNA glycosylases e.g. Q9F308|SCC80.11c putative DNA repair hydrolase (fragment) from Streptomyces coelicolor (306 aa),FASTA scores: opt: 894, E(): 6.1e-51, (51.05% identity in 282 aa overlap); O50606|MUTM|FPG_THETH formamidopyrimidine-DNA glycosylase from Thermus aquaticus (267 aa), FASTA scores: opt: 342, E(): 4.6e-15, (32.4% identity in 250 aa overlap); Q9RCW5|SCM10.34c putative formamidopyrimidine-DNA glycosylase from Streptomyces coelicolor (287 aa), FASTA scores: opt: 321, E(): 1.1e-13,(29.35% identity in 259 aa overlap); etc. Identical to AAK46839|MT2539 formamidopyrimidine-DNA glycosylase from Mycobacterium tuberculosis strain CDC1551. Also similar to other Mycobacterium tuberculosis DNA glycosylases e.g. MTCY71.37 (32.9% identity in 277 aa overlap). Belongs to the FPG family.; Possible DNA glycosylase 2767665 888500 Rv2464c Mycobacterium tuberculosis H37Rv Possible DNA glycosylase NP_216980.1 2766859 R 83332 CDS YP_177884.1 57116993 887225 complement(2767671..2768159) 1 NC_000962.3 Rv2465c, (MTV008.21c), len: 162 aa. RpiB,Ribose-5-phosphate isomerase, proven biochemically (see Roos et al., 2004) equivalent to AAK46840|MT2540 putative carbohydrate-phosphate isomerase from Mycobacterium tuberculosis strain CDC1551 (159 aa). Equivalent to Q9CBY1|ML1484 possible phosphopentose isomerase from Mycobacterium leprae (162 aa), FASTA scores: opt: 992, E(): 7.1e-59, (89.5% identity in 162 aa overlap). Also highly similar or similar to several diverse isomerases e.g. Q9L206|SC8E4.02c putative isomerase from Streptomyces coelicolor (159 aa), FASTA scores: opt: 661, E(): 6.1e-37,(61.45% identity in 153 aa overlap); P47636|Y396_MYCGE|MG396 hypothetical LACA/RPIB family protein from Mycoplasma genitalium (152 aa), FASTA scores: opt: 357, E(): 8.2e-17, (42% identity in 150 aa overlap); P53527|Y396_MYCPN|MPN595|MP247 hypothetical LACA/RPIB family protein from Mycoplasma pneumoniae (152 aa), FASTA scores: opt: 340, E(): 1.1e-15, (38.6% identity in 145 aa overlap); P26592|LACB_STAAU galactose-6-phosphate isomerase from Staphylococcus aureus (171 aa), FASTA scores: opt: 296, E(): 1e-12, (35.4% identity in 158 aa overlap) and P37351|RPIB_ECOLI ribose 5-phosphate isomerase b from Escherichia coli (149 aa), FASTA scores: opt: 262, E(): 1.6e-10, (32.2% identity in 146 aa overlap); etc. Could belong to the LACA/RPIB family.; Ribose-5-phosphate isomerase 2768159 rpiB 887225 rpiB Mycobacterium tuberculosis H37Rv Ribose-5-phosphate isomerase YP_177884.1 2767671 R 83332 CDS NP_216982.1 15609603 888214 complement(2768261..2768884) 1 NC_000962.3 Rv2466c, (MTV008.22c), len: 207 aa. Conserved protein (see citation below), equivalent to Q9CBY0|ML1485 hypothetical protein from Mycobacterium leprae (207 aa),FASTA scores: opt: 1154, E(): 1.1e-67, (80.6% identity in 206 aa overlap). Also highly similar to Q9L201|SC8E4A.04c hypothetical protein from Streptomyces coelicolor (216 aa),FASTA scores: opt: 789, E(): 4.6e-44, (57.9% identity in 213 aa overlap). Also similar to AAK46628|MT2344 hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (230 aa), FASTA scores: opt: 324, E(): 6.1e-14,(30.4% identity in 194 aa overlap). Contains PS00195 Glutaredoxin active site.; hypothetical protein 2768884 888214 Rv2466c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216982.1 2768261 R 83332 CDS YP_177885.1 57116994 887403 2768986..2771571 1 NC_000962.3 Rv2467, (MTV008.23), len: 861 aa. Probable pepN,aminopeptidase N, equivalent to Q9CBX9|ML1486 probable aminopeptidase from Mycobacterium leprae (862 aa), FASTA scores: opt: 4751,E(): 0, (83.3% identity in 862 aa overlap). Also highly similar to others e.g. Q11010|AMPN_STRLI|PEPN from Streptomyces lividans (857 aa),FASTA scores: opt: 2839, E(): 1.8e-170, (53.25% identity in 864 aa overlap); Q9L1Z2|PEPN from Streptomyces coelicolor (857 aa), FASTA scores: opt: 2834, E(): 3.8e-170, (53.1% identity in 864 aa overlap); P37896|AMPN_LACDL|PEPN from Lactobacillus delbrueckii (subsp. lactis) (842 aa), FASTA scores: opt: 719, E(): 2.4e-37, (31.65% identity in 439 aa overlap); etc. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Belongs to peptidase family M1 (zinc metalloprotease), also known as the PEPN subfamily. Note that previously known as pepD. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable aminopeptidase N PepN (Lysyl aminopeptidase) (LYS-AP) (alanine aminopeptidase) 2771571 pepN 887403 pepN Mycobacterium tuberculosis H37Rv Probable aminopeptidase N PepN (Lysyl aminopeptidase) (LYS-AP) (alanine aminopeptidase) YP_177885.1 2768986 D 83332 CDS NP_216984.1 15609605 887767 complement(2771644..2772147) 1 NC_000962.3 Rv2468c, (MTV008.24c), len: 167 aa. Conserved protein, highly similar to Mycobacterium leprae hypothetical proteins Q9CC58|ML1255 (163 aa), FASTA scores: opt: 859, E(): 1.6e-49, (81.2% identity in 165 aa overlap) and Q9X7B5|MLCB1610.16 (169 aa), FASTA scores: opt: 859,E(): 1.6e-49, (81.2% identity in 165 aa overlap). Also weak similarity with Q9X8D7|SCE39.14c putative GntR-family regulator from Streptomyces coelicolor (243 aa), FASTA scores: opt: 116, E(): 1.3, (30.1% identity in 156 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 2772147 887767 Rv2468c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216984.1 2771644 R 83332 CDS YP_007411168.1 448817295 14515866 complement(2772098..2772331) 1 NC_000962.3 Rv2468A, len: 77 aa. Conserved protein.; hypothetical protein 2772331 14515866 Rv2468A Mycobacterium tuberculosis H37Rv hypothetical protein YP_007411168.1 2772098 R 83332 CDS NP_216985.1 15609606 888591 complement(2772367..2773035) 1 NC_000962.3 Rv2469c, (MTV008.25c), len: 222 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9X7B4|MLCB1610.15|ML1254 from Mycobacterium leprae (215 aa), FASTA scores: opt: 1183, E(): 3.3e-70,(77.9% identity in 222 aa overlap); Q9L1Y0|SC8E4A.25c from Streptomyces coelicolor (178 aa), FASTA scores: opt: 589,E(): 1.7e-31, (53.4% identity in 161 aa overlap) (N-terminal region is shorter 50 aa approximately); Q9RRS6|DR2409 conserved hypothetical protein from Deinococcus radiodurans (186 aa), FASTA scores: opt: 440,E(): 9.6e-22, (42.25% identity in 168 aa overlap) (N-terminal region is shorter 30 aa approximately); etc.; hypothetical protein 2773035 888591 Rv2469c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216985.1 2772367 R 83332 CDS NP_216986.1 15609607 887743 2773178..2773564 1 NC_000962.3 Rv2470, (MTV008.26), len: 128 aa. glbO, globin-like protein, highly similar to Q9CC59|GLBO|ML1253 hemoglobin-like (oxygen carrier) from Mycobacterium leprae (128 aa), FASTA scores: opt: 767, E(): 4e-47, (88.1% identity in 126 aa overlap); Q9X7B3|MLCB1610.14c putative globin from Mycobacterium leprae (131 aa); Q9L250|SC6D10.14 putative globin from Streptomyces coelicolor (137 aa),FASTA scores: opt: 466, E(): 5.7e-26, (53.6% identity in 125 aa overlap). Also similar to O31607 YJBI protein from Bacillus subtilis (132 aa), FASTA scores: opt: 294, E(): 6.6e-14; (39.85% identity in 128 aa overlap). Could belong to protozoan/cyanobacterial globin family protein.; Globin (oxygen-binding protein) GlbO 2773564 glbO 887743 glbO Mycobacterium tuberculosis H37Rv Globin (oxygen-binding protein) GlbO NP_216986.1 2773178 D 83332 CDS NP_216987.1 15609608 887393 2773564..2775204 1 NC_000962.3 Rv2471, (MTV008.27), len: 546 aa. Probable aglA,maltase (alpha-glucosidase), highly similar or similar to several e.g. Q60027|AGLA from Thermomonospora curvata (544 aa), FASTA scores: opt: 2071, E(): 4e-116, (57.7% identity in 525 aa overlap); Q9KZE3|AGLAE from Streptomyces coelicolor (534 aa), FASTA scores: opt: 1475, E(): 1.5e-80,(50.1% identity in 537 aa overlap); O86874|AGLA from Streptomyces lividans (534 aa), FASTA scores: opt: 1473,E(): 2e-80, (50.1% identity in 537 aa overlap); etc. Seems to belong to family 13 of glycosyl hydrolases, also known as the alpha-amylase family.; Probable alpha-glucosidase AglA (maltase) (glucoinvertase) (glucosidosucrase) (maltase-glucoamylase) (lysosomal alpha-glucosidase) (acid maltase) 2775204 aglA 887393 aglA Mycobacterium tuberculosis H37Rv Probable alpha-glucosidase AglA (maltase) (glucoinvertase) (glucosidosucrase) (maltase-glucoamylase) (lysosomal alpha-glucosidase) (acid maltase) NP_216987.1 2773564 D 83332 CDS NP_216988.1 15609609 887255 2775272..2775565 1 NC_000962.3 Rv2472, (MTV008.28), len: 97 aa. Conserved hypothetical protein, showing some similarity to O53451|Rv1103c|MTV017.56c from Mycobacterium tuberculosis strain H37Rv (106 aa), FASTA scores: opt: 135, E(): 0.026,(45.85% identity in 72 aa overlap); and AAK45393|MT1135 hypothetical 11.4 KDA protein from Mycobacterium tuberculosis strain CDC1551 (78 aa) FASTA scores: opt: 139,E(): 0.011, (45.35% identity in 75 aa overlap).; hypothetical protein 2775565 887255 Rv2472 Mycobacterium tuberculosis H37Rv hypothetical protein NP_216988.1 2775272 D 83332 CDS NP_216989.1 15609610 888163 2775568..2776284 1 NC_000962.3 Rv2473, (MTV008.29), len: 238 aa. Possible pro-,ala-rich membrane protein, with possible transmembrane domain around aa 81-104.; Possible alanine and proline rich membrane protein 2776284 888163 Rv2473 Mycobacterium tuberculosis H37Rv Possible alanine and proline rich membrane protein NP_216989.1 2775568 D 83332 CDS NP_216990.1 15609611 888606 complement(2776316..2776969) 1 NC_000962.3 Rv2474c, (MTV008.30c), len: 217 aa. Hypothetical protein. Shows weak similarity with Q9L246|SC6D10.18c hypothetical 24.9 KDA protein from Streptomyces coelicolor (238 aa), FASTA scores: opt: 111, E(): 5.6, (30% identity in 233 aa overlap), blastp scores: Score= 135, E= 3.5e-07,P= 3.5e-07, Identities= 55/182 (30%).; hypothetical protein 2776969 888606 Rv2474c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216990.1 2776316 R 83332 CDS NP_216991.1 15609612 887793 complement(2776975..2777391) 1 NC_000962.3 Rv2475c, (MTV008.31c), len: 138 aa. Conserved protein, showing similarity with Q9L245|SC6D10.19c hypothetical 16.2 KDA protein from Streptomyces coelicolor (136 aa), FASTA scores: opt: 236, E(): 1.9e-09, (34.1% identity in 126 aa overlap). Also some similarity with AAK44393|Z97050|MTCI28_3 conserved hypothetical protein from Mycobacterium tuberculosis cosmid I (151 aa), FASTA scores: opt: 147, E(): 0.00025, (29.2% identity in 120 aa overlap).; hypothetical protein 2777391 887793 Rv2475c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216991.1 2776975 R 83332 CDS NP_216992.1 15609613 887437 complement(2777388..2782262) 1 NC_000962.3 Rv2476c, (MTV008.32c), len: 1624 aa. Probable gdh,glutamate dehydrogenase. Highly similar to Q9X7B2|MLCB1610.10|ML1249 hypothetical 177.9 KDA protein from Mycobacterium leprae (1622 aa), FASTA scores: opt: 8630,E(): 0, (81.45% identity in 1634 aa overlap). But highly similar to Q9F0J1|GDH NAD-glutamate dehydrogenase from Streptomyces clavuligerus (1651 aa), FASTA scores: opt: 3833, E(): 0, (45.8% identity in 1600 aa overlap); (see Minambres et al., 2000). Also similar with others e.g. AAG53963|PA3068|GDHB hypothetical (NAD(+)-dependent glutamate dehydrogenase from Pseudomonas aeruginosa (1620 aa), FASTA scores: opt: 2214, E(): 1e-124, (40.1% identity in 1561 aa overlap) (see Lu & Abdelal 2001); and Q9Y8G5|GDHB NAD-specific glutamate dehydrogenase from Agaricus bisporus (1029 aa), FASTA scores: opt: 194, E(): 0.00099, (22.7% identity in 647 aa overlap) (see Kersten et al., 1999); etc. Contains possible Helix-turn-helix motif at aa 1568 to 1589 (score 1098, +2.93 SD).; Probable NAD-dependent glutamate dehydrogenase Gdh (NAD-Gdh) (NAD-dependent glutamic dehydrogenase) 2782262 gdh 887437 gdh Mycobacterium tuberculosis H37Rv Probable NAD-dependent glutamate dehydrogenase Gdh (NAD-Gdh) (NAD-dependent glutamic dehydrogenase) NP_216992.1 2777388 R 83332 CDS NP_216993.1 15609614 887757 complement(2782366..2784042) 1 NC_000962.3 Rv2477c, (MTV008.33c), len: 558 aa. Probable ATP binding protein ABC-transporter (see citation below),probably involved in macrolide transport, equivalent to Q9X7B1|MLCB1610.09|ML1248 putative ABC transporter ATP-binding protein from Mycobacterium leprae (556 aa) FASTA scores: opt: 3448, E(): 3.8e-176, (92.3% identity in 557 aa overlap). Also highly similar to many ATP binding proteins e.g. Q9L244|SC6D10.20c putative ABC transporter ATP-binding protein from Streptomyces coelicolor (547 aa),FASTA scores: opt: 2937, E(): 5.6e-149, (79.5% identity in 551 aa overlap); AAK24119|CC2148 ABC transporter ATP-binding protein from Caulobacter crescentus (555 aa),FASTA scores: opt: 2175, E(): 1.9e-108, (59.4% identity in 557 aa overlap); Q9HVJ1 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa (554 aa), FASTA scores: opt: 2054, E(): 5.1e-102, (56.9% identity in 559 aa overlap); etc. Contains 2 x PS00017 ATP/GTP-binding site motif A (P-loop), 2 x PS00211 ABC transporters family signature, and probable coiled-coil from aa 273 to 311. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable macrolide-transport ATP-binding protein ABC transporter 2784042 887757 Rv2477c Mycobacterium tuberculosis H37Rv Probable macrolide-transport ATP-binding protein ABC transporter NP_216993.1 2782366 R 83332 CDS NP_216994.1 15609615 887304 complement(2784123..2784608) 1 NC_000962.3 Rv2478c, (MTV008.34c), len: 161 aa. Conserved hypothetical protein, with weak similarity with many single-strand binding proteins e.g. Q9X8U3|SCH24.29 putative single-strand binding protein from Streptomyces coelicolor (199 aa), FASTA scores: opt: 246, E(): 4.5e-08,(31.5% identity in 162 aa overlap); P46390|SSB_MYCLE|ML2684|MLCB1913.20c single-strand binding protein (SSB) (helix-destabilizing protein) from Mycobacterium leprae (168 aa), FASTA scores: opt: 239, E(): 1e-07, (30.8% identity in 146 aa overlap); P18310|SSBF_ECOLI single-strand binding protein from Escherichia coli (178 aa), FASTA scores: opt: 116, E(): 2.9, (25.7% identity in 140 aa overlap); etc. Also similarity with Rv0054|P71711|MTCY21D4.17|SSB_MYCTU probable single-strand binding protein from M. tuberculosis (164 aa), FASTA scores: opt: 234, E(): 2e-07, (31.75% identity in 148 aa overlap). N-terminus shorter 8 aa from AAK46855|MT2553 single-strand DNA binding protein from Mycobacterium tuberculosis strain CDC1551.; hypothetical protein 2784608 887304 Rv2478c Mycobacterium tuberculosis H37Rv hypothetical protein NP_216994.1 2784123 R 83332 CDS NP_216995.2 448824787 887201 complement(2784657..>2785643) 1 NC_000962.3 Rv2479c, (MTV008.35c), len: 328 aa. Probable transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2480c and Rv2479c, the sequence UUUUAAAG (directly upstream of Rv2479c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (- 18 aa).; Probable transposase >2785643 887201 Rv2479c Mycobacterium tuberculosis H37Rv Probable transposase NP_216995.2 2784657 R 83332 CDS NP_216996.1 15609617 887328 complement(2785592..2785918) 1 NC_000962.3 Rv2480c, (MTV008.36c), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2480c and Rv2479c, the sequence UUUUAAAG (directly upstream of Rv2479c) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Possible transposase for insertion sequence element IS6110 (fragment) 2785918 887328 Rv2480c Mycobacterium tuberculosis H37Rv Possible transposase for insertion sequence element IS6110 (fragment) NP_216996.1 2785592 R 83332 CDS NP_216997.1 15609618 887462 complement(2786575..2786898) 1 NC_000962.3 Rv2481c, (MTV008.37c), len: 107 aa. Hypothetical unknown protein.; Hypothetical protein 2786898 887462 Rv2481c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_216997.1 2786575 R 83332 CDS NP_216998.1 15609619 887848 complement(2786914..2789283) 1 NC_000962.3 Rv2482c, (MT2555, MTV008.38c), len: 789 aa. Probable plsB2, glycerol-3-phosphate acyltransferase, highly similar to Q9X7B0|PLSB_MYCLE probable glycerol-3-phosphate acyltransferase from Mycobacterium leprae (775 aa), FASTA scores: opt: 4210, E(): 0, (80.7% identity in 783 aa overlap). Also similar to others e.g. P00482|PLSB_ECOLI from Escherichia coli (806 aa), FASTA scores: opt: 521,E(): 3e-24, (24.35 identity in 612 aa overlap); Q9CLN7|PLSB_PASMU from Pasteurella multocida (809 aa),FASTA scores: opt: 529, E(): 9.7e-25, (27.05% identity in 540 aa overlap); Q9KVP8|PLSB_VIBCH from Vibrio cholerae (811 aa), FASTA scores: opt: 510, E(): 1.4e-23, (26.0% identity in 639 aa overlap); etc. Also highly similar to Q10775|PLSB1|Rv1551|MTCY48.14c from M. tuberculosis (621 aa), FASTA scores: opt: 1013, E(): 1.5e-54, (34.65% identity in 586 aa overlap). Belongs to the GPAT/DAPAT family.; Probable glycerol-3-phosphate acyltransferase PlsB2 (GPAT) 2789283 plsB2 887848 plsB2 Mycobacterium tuberculosis H37Rv Probable glycerol-3-phosphate acyltransferase PlsB2 (GPAT) NP_216998.1 2786914 R 83332 CDS NP_216999.1 15609620 887744 complement(2789280..2791022) 1 NC_000962.3 Rv2483c, (MTV008.39c), len: 580 aa. Possible plsC, a transmembrane phospholipid biosynthesis bifunctional enzyme, including L-3-phosphoserine phosphatase and 1-acyl-Sn-glycerol-3-phosphate acyltransferase , equivalent to Q9X7A9|PLSC|ML1245 putative acyltransferase from Mycobacterium leprae (579 aa), FASTA scores: opt: 2835,E(): 9.2e-153, (77.15% identity in 573 aa overlap). C-terminal end is similar to many 1-acyl-SN-glycerol-3-phosphate acyltransferases (lysophosphatidic acidacyltransferases) e.g. Q9SDQ2 from Limnanthes floccosa (281 aa), FASTA scores: opt: 378, E(): 3.1e-14, (30.0% identity in 230 aa overlap) and Q42868|PLSC_LIMAL from Limnanthes alba (White meadowfoam) (281 aa), FASTA scores: opt: 374, E(): 5.2e-14, (30.55% identity in 221 aa overlap); and the N-terminal end is similar to many SerB family proteins e.g. AAK44749|MT0526 from Mycobacterium tuberculosis strain CDC1551 (308 aa),FASTA scores: opt: 356, E(): 5.8e-13, (32.5% identity in 298 aa overlap) and Q49823|ML2424 from Mycobacterium leprae (300 aa), FASTA scores: opt: 346, E(): 2.1e-12, (32.0% identity in 278 aa overlap). So belongs to the 1-acyl-SN-glycerol-3-phosphate acyltransferase family and may belong to the SerB family.; Possible transmembrane phospholipid biosynthesis bifunctional enzyme PlsC: putative L-3-phosphoserine phosphatase (O-phosphoserine phosphohydrolase) (PSP) (pspase) + 1-acyl-SN-glycerol-3-phosphate acyltransferase (1-AGP acyltransferase) (1-AGPAT) (lysophosphatidic acid acyltransferase) (LPAAT) 2791022 plsC 887744 plsC Mycobacterium tuberculosis H37Rv Possible transmembrane phospholipid biosynthesis bifunctional enzyme PlsC: putative L-3-phosphoserine phosphatase (O-phosphoserine phosphohydrolase) (PSP) (pspase) + 1-acyl-SN-glycerol-3-phosphate acyltransferase (1-AGP acyltransferase) (1-AGPAT) (lysophosphatidic acid acyltransferase) (LPAAT) NP_216999.1 2789280 R 83332 CDS NP_217000.1 15609621 888623 complement(2791019..2792494) 1 NC_000962.3 Rv2484c, (MTV008.40c), len: 491 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), highly similar or similar to many Mycobacterial hypothetical proteins e.g. Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 2459, E(): 3e-138, (75.15% identity in 483 aa overlap); O53304|YU87_MYCTU|Rv3087|MTV013.08 from Mycobacterium tuberculosis (472 aa), FASTA scores: opt: 527, E(): 8.1e-24, (29.1% identity in 485 aa overlap); O53305|YU88_MYCTU|Rv3088|MT3173|MTV013.09 from Mycobacterium tuberculosis (474 aa), FASTA scores: opt: 370, E(): 1.6e-14, (26.05% identity in 422 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 2792494 888623 Rv2484c Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_217000.1 2791019 R 83332 CDS NP_217001.1 15609622 887876 complement(2792723..2793988) 1 NC_000962.3 Rv2485c, (MTV008.41c), len: 421 aa. Probable lipQ,carboxylesterase protein (lipase). Similar (greater at the C-terminal end) to AAK46626|MT2342 putative carboxylesterase from Mycobacterium tuberculosis strain CDC1551 (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60,(46.25% identity in 428 aa overlap); and Q50681|Rv2284|MTCY339.26c hypothetical protein from M. tuberculosis strain H37Rv (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60, (46.25% identity in 428 aa overlap). Also similar in part to other putative lipases/esterases e.g. AAK44451|MT0230 from Mycobacterium tuberculosis strain CDC1551 (403 aa), FASTA scores: opt: 763, E(): 4.6e-38,(37.95% identity in 390 aa overlap); Q9RY19|DR0133 from Deinococcus radiodurans (296 aa), FASTA scores: opt: 392,E(): 4e-16, (33.7% identity in 276 aa overlap); Q9Z545|SC9B2.14 from Streptomyces coelicolor (502 aa) FASTA scores: opt: 279, E(): 3.2e-09, (31.15% identity in 292 aa overlap); etc.; Probable carboxylesterase LipQ 2793988 lipQ 887876 lipQ Mycobacterium tuberculosis H37Rv Probable carboxylesterase LipQ NP_217001.1 2792723 R 83332 CDS NP_217002.1 15609623 887894 2794350..2795120 1 NC_000962.3 Rv2486, (MTV008.42), len: 256 aa. Probable echA14,enoyl-CoA hydratase, similar to others e.g. P24162|ECHH_RHOCA2|FADB1 from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores; opt: 453, E(): 3.8e-23, (39.4% identity in 259 aa overlap); Q9ETY7|PACA|PAAG from Azoarcus evansii (273 aa), FASTA scores: opt: 404, E(): 5.7e-17, (37.5% identity in 224 aa overlap); P77467|PAAG_ECOLI from Escherichia coli (262 aa),FASTA scores: opt: 401, E(): 8.3e-17, (36.3% identity in 259 aa overlap); etc. Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family.; Probable enoyl-CoA hydratase EchA14 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 2795120 echA14 887894 echA14 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA14 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_217002.1 2794350 D 83332 CDS YP_177886.1 57116995 887909 complement(2795301..2797385) 1 NC_000962.3 Rv2487c, (MTV008.43c), len: 694 aa. PE_PGRS42,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of Gly-rich proteins (see citation below),similar to many e.g. AAK47245|MT2919 PE_PGRS family protein from Mycobacterium tuberculosis strain CDC1515 (663 aa),FASTA scores: opt: 2317, E(): 2.3e-84, (58.35% identity in 622 aa overlap); etc.; PE-PGRS family protein PE_PGRS42 2797385 PE_PGRS42 887909 PE_PGRS42 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS42 YP_177886.1 2795301 R 83332 CDS NP_217004.1 15609625 887997 complement(2797467..2800880) 1 NC_000962.3 Rv2488c, (MTV008.44c), len: 1137 aa. Probable transcriptional regulatory protein, belonging to luxR family, similar to many in Mycobacterium tuberculosis e.g. AAK44621|MT0399 from strain CDC1551 (1092 aa) FASTA scores: opt: 3767, E(): 1.8e-211, (56.75% identity in 1093 aa overlap); O53720|Rv0386|MTV036.21 from strain H37Rv (1085 aa), FASTA scores: opt: 3756, E(): 7.6e-211, (56.75% identity in 1089 aa overlap); AAK45665|MT1402 from strain CDC1551 (1159 aa), FASTA scores: opt: 3395, E(): 8.2e-190,(52.0% identity in 1093 aa overlap); etc. Also similar to transcriptional regulatory proteins luxR-family from other organisms e.g. Q9CBP3|ML1753 from Mycobacterium leprae (1106 aa), FASTA scores: opt: 2823, E(): 1.5e-156, (50.35% identity in 1116 aa overlap); Q9KYF4|SCD72A.02 from Streptomyces coelicolor (1114 aa), FASTA scores: opt: 915,E(): 1.7e-45, (30.7% identity in 1143 aa overlap); etc. Some similarity with Q9KXP6|SC9C5.28 hypothetical 81.8 KDA protein from Streptomyces coelicolor (750 aa), FASTA scores: opt: 1085, E(): 1.6e-55, (35.45% identity in 722 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature, probable coiled-coil from aa 585 to 616 and probable helix-turn-helix motif at aa 1086 to 1107 (score 1206, +3.29 SD). Belongs to the LuxR/UhpA family of transcriptional regulators.; Probable transcriptional regulatory protein (LuxR-family) 2800880 887997 Rv2488c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (LuxR-family) NP_217004.1 2797467 R 83332 CDS NP_217005.1 15609626 888937 complement(2800846..2801145) 1 NC_000962.3 Rv2489c, (MTV008.45c), len: 99 aa. Hypothetical unknown ala-rich protein.; Hypothetical alanine rich protein 2801145 888937 Rv2489c Mycobacterium tuberculosis H37Rv Hypothetical alanine rich protein NP_217005.1 2800846 R 83332 CDS YP_177887.1 57116996 887941 complement(2801254..2806236) 1 NC_000962.3 Rv2490c, (MTV008.46c), len: 1660 aa. PE_PGRS43,Member of the Mycobacterium tuberculosis PE family,PGRS-subfamily of Gly-rich proteins (see Brennan and Delogu, 2002), similar to many e.g. AAK47971|MT3612.1 PE_PGRS family protein from Mycobacterium tuberculosis strain CDC1551 (1715 aa), FASTA scores: opt: 5161, E(): 1.5e-187, (51.7% identity in 1752 aa overlap); etc.; PE-PGRS family protein PE_PGRS43 2806236 PE_PGRS43 887941 PE_PGRS43 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS43 YP_177887.1 2801254 R 83332 CDS NP_217007.1 15609628 887780 2806665..2807288 1 NC_000962.3 Rv2491, (MTV008.47), len: 207 aa. Conserved hypothetical protein, similar in part to other hypothetical proteins e.g. O29139|AF1126 from Archaeoglobus fulgidus (151 aa), FASTA scores: opt: 293, E(): 2.8e-11, (42.85% identity in 126 aa overlap); O66531|AQ_134 from Aquifex aeolicus (151 aa), FASTA scores: opt: 261, E(): 2.6e-09,(37.75% identity in 106 aa overlap); Q9HKU3|TA0501 from Thermoplasma acidophilum (161 aa), FASTA scores: opt: 260,E(): 3.2e-09, (35.9% identity in 117 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 2807288 887780 Rv2491 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217007.1 2806665 D 83332 CDS NP_217008.1 15609629 887436 2807278..2808030 1 NC_000962.3 Rv2492, (MTV008.48), len: 250 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 2808030 887436 Rv2492 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217008.1 2807278 D 83332 CDS NP_217009.1 15609630 887480 2808083..2808304 1 NC_000962.3 Rv2493, (MTV008.49), len: 73 aa. Possible vapB38,antitoxin, part of toxin-antitoxin (TA) operon with Rv2494,see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strain e.g. Rv3321c|MTV016.21c hypothetical 8.8 KDA protein from Mycobacterium tuberculosis strain H37Rv (80 aa). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible antitoxin VapB38 2808304 vapB38 887480 vapB38 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB38 NP_217009.1 2808083 D 83332 CDS NP_217010.1 15609631 887700 2808310..2808735 1 NC_000962.3 Rv2494, (MTV008.50), len: 141 aa. Possible vapC38,toxin, part of toxin-antitoxin (TA) operon with Rv2493,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. P95023|EMBL:Z83863|MTCY159.26|Rv2530c (139 aa) FASTA scores: opt: 380 E(): 6.6e-19, (48.0% identity in 125 aa overlap); O53372|Rv3320c|MTV016.20c (142 aa), etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible toxin VapC38 Contains PIN domain 2808735 vapC38 887700 vapC38 Mycobacterium tuberculosis H37Rv Possible toxin VapC38 Contains PIN domain NP_217010.1 2808310 D 83332 CDS NP_217011.1 15609632 888237 complement(2808758..2809939) 1 NC_000962.3 Rv2495c, (MTCY07A7.01c-MTV008.51c), len: 393 aa. Probable bkdC, branched-chain keto acid dehydrogenase, E2 component, similar to others e.g. Q9XA49|SCGD3.30c from Streptomyces coelicolor (491 aa) FASTA scores: opt: 615,E(): 1.2e-28, (36.45% identity in 491 aa overlap; several gaps); P19262|ODO2_YEAST|KGD2|YDR148C|YD8358.05c from Saccharomyces cerevisiae (Baker's yeast) (463 aa) FASTA scores: opt: 533, E(): 7.1e-24, (28.55% identity in 396 aa overlap); Q9HN75|DSA|VNG2219G from Halobacterium sp. strain NRC-1 (478 aa), FASTA scores: opt: 521, E(): E(): 3.7e-23,(30.25% identity in 486 aa overlap; in part); etc. Belongs to the 2-oxoacid dehydrogenase family. Alternative nucleotide at position 2809621 (T->C; T107A) has been observed. LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). Previously known as pdhC.; Probable branched-chain keto acid dehydrogenase E2 component BkdC 2809939 bkdC 888237 bkdC Mycobacterium tuberculosis H37Rv Probable branched-chain keto acid dehydrogenase E2 component BkdC NP_217011.1 2808758 R 83332 CDS NP_217012.1 15609633 888571 complement(2809936..2810982) 1 NC_000962.3 Rv2496c, (MTCY07A7.02c), len: 348 aa. Probable bkdB,branched-chain keto acid dehydrogenase E1 component, beta subunit, similar to others e.g. Q9Y8I6||PDHB from Halobacterium volcanii (Haloferax volcanii) (327 aa) FASTA scores: opt: 1050, E(): 6.4e-60, (49.7% identity in 324 aa overlap); Q9KG98|BH0214 from Bacillus halodurans (328 aa),FASTA scores: opt: 987, E(): 6.9e-56, (45.7% identity in 324 aa overlap); Q9HN76|PDHB|VNG2218G from Halobacterium sp. strain NRC-1 (297 aa), FASTA scores: opt: 968, E(): 1.1e-54, (51.2% identity in 297 aa overlap); P21874|ODPB_BACST|PDHB pyruvate dehydrogenase E1 component from Bacillus stearothermophilus (324 aa), FASTA scores: opt: 951, E(): 1.4e-53, (47.6% identity in 321 aa overlap); etc. Also similar to Q9XA61|SCGD3.17c putative branched-chain alpha keto acid dehydrogenase E1, beta subunit (2-oxoisovalerate dehydrogenase) from Streptomyces coelicolor, (326 aa), FASTA scores: opt: 1178, E(): 4.1e-68, (55.0% identity in 322 aa overlap); Q9XA48|SCGD3.31c putative branched-chain alpha keto acid dehydrogenase E1 beta subunit from Streptomyces coelicolor (334 aa), FASTA scores: opt: 1173, E(): 8.8e-68, (55.6% identity in 320 aa overlap); Q53593|BKDB E1-beta branched-chain alpha keto acid dehydrogenase from Streptomyces avermitilis (334 aa), FASTA scores: opt: 1132,E(): 3.7e-65, (55.0% identity in 320 aa overlap); etc. Previously known as pdhB.; Probable branched-chain keto acid dehydrogenase E1 component, beta subunit BkdB 2810982 bkdB 888571 bkdB Mycobacterium tuberculosis H37Rv Probable branched-chain keto acid dehydrogenase E1 component, beta subunit BkdB NP_217012.1 2809936 R 83332 CDS NP_217013.1 15609634 888583 complement(2810993..2812096) 1 NC_000962.3 Rv2497c, (MTCY07A7.03c), len: 367 aa. Probable bkdA,branched-chain keto acid dehydrogenase E1 component, alpha subunit, similar to many e.g. Q9Y8I5|PDHA from Halobacterium volcanii (Haloferax volcanii) (368 aa) FASTA scores: opt: 961, E(): 1.3e-52, (45.6% identity in 351 aa overlap); BAB40585 from Bacillus sp. UTB2301 (356 aa) FASTA scores: opt: 947, E(): 9.1e-52, (43.1% identity in 355 aa overlap); Q9KG99|BH0213 from Bacillus halodurans (367 aa),FASTA scores: opt: 896, E(): 1.4e-48, (42.65% identity in 340 aa overlap); etc. Also similar to several putative branched-chain alpha keto acid dehydrogenases E1, beta subunit, alternate name : 2-oxoisovalerate dehydrogenase,e.g. Q53592|BKDA from Streptomyces avermitilis (381 aa),FASTA scores: opt: 980, E(): 8.5e-54, (45.65% identity in 370 aa overlap); etc. Previously known as pdhA.; Probable branched-chain keto acid dehydrogenase E1 component, alpha subunit BkdA 2812096 bkdA 888583 bkdA Mycobacterium tuberculosis H37Rv Probable branched-chain keto acid dehydrogenase E1 component, alpha subunit BkdA NP_217013.1 2810993 R 83332 CDS NP_217014.1 15609635 887466 complement(2812355..2813176) 1 NC_000962.3 Rv2498c, (MTCY07A7.04c), len: 273 aa. Probable citE,citrate lyase, beta subunit, similar to others e.g. Q9S3L3|cite from Corynebacterium glutamicum (Brevibacterium flavum) (217 aa), FASTA scores: opt: 565, E(): 1.5e-28,(41.85% identity in 215 aa overlap); Q9HRM8|cite|VNG0627G from Halobacterium sp. strain NRC-1 (303 aa), FASTA scores: opt: 535, E(): 1.5e-26, (41.65% identity in 276 aa overlap); Q9S2U9|SC4G6.02 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 426, E(): 1e-19, (37.6% identity in 274 aa overlap); P77770|CILB_ECOLI from Escherichia coli (307 aa), FASTA scores: opt: 265, E(): 1.5e-10, (32.8% identity in 265 aa overlap); etc. Also similar to Rv3075c|MTCY22D7.06 from Mycobacterium tuberculosis, FASTA score: (35.2% identity in 264 aa overlap).; Probable citrate (pro-3S)-lyase (beta subunit) CitE (citrase) (citratase) (citritase) (citridesmolase) (citrase aldolase) 2813176 citE 887466 citE Mycobacterium tuberculosis H37Rv Probable citrate (pro-3S)-lyase (beta subunit) CitE (citrase) (citratase) (citritase) (citridesmolase) (citrase aldolase) NP_217014.1 2812355 R 83332 CDS NP_217015.1 15609636 888584 complement(2813173..2813730) 1 NC_000962.3 Rv2499c, (MTCY07A7.05c), len: 185 aa. Possible oxidase regulatory-related protein, similar to many maoC monoamine oxidase regulatory protein e.g. Q9RUZ1|DR1239 MAOC-related protein from Deinococcus radiodurans (160 aa),FASTA scores: opt: 519, E(): 7.6e-28, (58.1% identity in 148 aa overlap); BAB48392|MLR0905 Probable monoamine oxidase regulatory protein from Rhizobium loti (Mesorhizobium loti) (150 aa), FASTA scores: opt: 480, E(): 2.9e-25, (49.0% identity in 149 aa overlap); Q9HN18|MAOC1|VNG2290G monoamine oxidase regulatory-like from Halobacterium sp. strain NRC-1 (208 aa), FASTA scores: opt: 419, E(): 4.6e-21, (45.6% identity in 158 aa overlap); P77455|MAOC_ECOLI|PAAZ|B1387 MaoC protein (Phenylacetic acid degradation protein paaZ) from Escherichia coli strain K12 (681 aa), FASTA scores: opt: 252, E(): 1.9e-09, (36.0% identity in 172 aa overlap); etc. But also similar to other proteins with different putative functions e.g. Q9HRM9|MAOC2|VNG0626G molybdenum cofactor biosynthesis protein from Halobacterium sp strain NRC-1 (157 aa), FASTA scores: opt: 380, E(): 1.5e-18, (45.75% identity in 153 aa overlap); Q9KIF1 FKBR2 from Streptomyces hygroscopicus var. ascomyceticus (175 aa), FASTA scores: opt: 355, E(): 7.6e-17, (42.0% identity in 150 aa overlap); CAC36828|Q99Q03|SAPE Spore associated protein from Streptomyces coelicolor (174 aa), FASTA scores: opt: 318,E(): 2.2e-14, (41.45% identity in 152 aa overlap); etc.; Possible oxidase regulatory-related protein 2813730 888584 Rv2499c Mycobacterium tuberculosis H37Rv Possible oxidase regulatory-related protein NP_217015.1 2813173 R 83332 CDS NP_217016.1 15609637 888541 complement(2813727..2814911) 1 NC_000962.3 Rv2500c, (MTCY07A7.06c), len: 394 aa. Possible fadE19 (alternate gene name: mmgC), acyl-CoA dehydrogenase,similar to many e.g. Q9XCG6|ACDH from Streptomyces coelicolor (386 aa), FASTA scores: opt: 1714, E(): 1.1e-98,(69.45% identity in 383 aa overlap); Q9XCG5|ACDH from Streptomyces avermitilis (386 aa), FASTA scores: opt: 1713,E(): 1.3e-98, (70.0% identity in 383 aa overlap); Q9L7W5|FENK from Bacillus subtilis (370 aa), FASTA scores: opt: 1094, E(): 2.3e-60, (48.4% identity in 372 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family.; Possible acyl-CoA dehydrogenase FadE19 (MMGC) 2814911 fadE19 888541 fadE19 Mycobacterium tuberculosis H37Rv Possible acyl-CoA dehydrogenase FadE19 (MMGC) NP_217016.1 2813727 R 83332 CDS NP_217017.1 15609638 887309 complement(2814916..2816880) 1 NC_000962.3 Rv2501c, (MTCY07A7.07c, P46401), len: 654 aa. Probable accA1 (alternate gene name: bccA),acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], similar to others eg Q9L076|FABG from Streptomyces coelicolor (646 aa), FASTA scores: opt: 2071,E(): 1e-113, (57.8% identity in 659 aa overlap); AAK24139|Q9A6C6|CC2168 from Caulobacter crescentus (654 aa), FASTA scores: opt: 1754, E(): 3.7e-95, (47.2% identity in 661 aa overlap); etc. Contains PS00188 Biotin-requiring enzymes attachment site, PS00866 Carbamoyl-phosphate synthase subdomain signature 1, and PS00867 Carbamoyl-phosphate synthase subdomain signature 2.; Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA1: biotin carboxylase + biotin carboxyl carrier protein (BCCP) 2816880 accA1 887309 accA1 Mycobacterium tuberculosis H37Rv Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA1: biotin carboxylase + biotin carboxyl carrier protein (BCCP) NP_217017.1 2814916 R 83332 CDS NP_217018.1 15609639 887168 complement(2816885..2818474) 1 NC_000962.3 Rv2502c, (MTCY07A7.08c), len: 529 aa. Probable accD1, acetyl-/propionyl-CoA carboxylase (beta subunit) ,similar, but with N-terminus shorter, to Q9L077|ACCD1 from Streptomyces coelicolor (538 aa), FASTA scores: opt: 2747,E(): 1.9e-159, (77.9% identity in 516 aa overlap). Also similar to others e.g. AAK24141|CC2170 from Caulobacter crescentus (530 aa), FASTA scores: opt: 2413, E(): 3.8e-139, (69.4% identity in 529 aa overlap); BAB54131|MLL7731 from Rhizobium loti (537 aa), FASTA scores: opt: 2399, E(): 2.7e-138, (67.4% identity in 527 aa overlap); etc. Could belong to the ACCD/PCCB family.; Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD1 2818474 accD1 887168 accD1 Mycobacterium tuberculosis H37Rv Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD1 NP_217018.1 2816885 R 83332 CDS NP_217019.1 15609640 888502 complement(2818471..2819127) 1 NC_000962.3 Rv2503c, (MTCY07A7.09c, MT2578), len: 218 aa. Probable scoB, 3-oxo acid:CoA transferase, beta subunit (succinyl-CoA:3-ketoacid-CoA transferase). Highly similar to others e.g. Q9XAM8|SC4C6.12c from Streptomyces coelicolor (217 aa), FASTA scores: opt: 1048, E(): 2.6e-60,(73.9% identity in 207 aa overlap); Q9XD82|PCAJ from Streptomyces sp. 2065 (214 aa), FASTA scores: opt: 1031,E(): 3.2e-59, (70.8% identity in 209 aa overlap); AAK53493|LPSJ from Xanthomonas campestris (pv. campestris) (212 aa), FASTA scores: opt: 886, E(): 6.6e-50, (62.5% identity in 208 aa overlap); P42316|SCOB_BACSU from Bacillus subtilis (216 aa), FASTA scores: opt: 820, E(): 1.2e-45, (58.2% identity in 201 aa overlap); etc. Belongs to the 3-oxoacid CoA-transferase subunit B family.; Probable succinyl-CoA:3-ketoacid-coenzyme A transferase (beta subunit) ScoB (3-oxo-acid:CoA transferase) (OXCT B) (succinyl CoA:3-oxoacid CoA-transferase) 2819127 scoB 888502 scoB Mycobacterium tuberculosis H37Rv Probable succinyl-CoA:3-ketoacid-coenzyme A transferase (beta subunit) ScoB (3-oxo-acid:CoA transferase) (OXCT B) (succinyl CoA:3-oxoacid CoA-transferase) NP_217019.1 2818471 R 83332 CDS NP_217020.1 15609641 888503 complement(2819124..2819870) 1 NC_000962.3 Rv2504c, (MT2579, MTCY07A7.10c), len: 248 aa. Probable scoA, succinyl-CoA:3-ketoacid-Coenzyme A transferase, alpha subunit (3-oxo acid:CoA transferase). Highly similar to others e.g. Q9XAM7|SC4C6.13c from Streptomyces coelicolor (260 aa), FASTA scores: opt: 1130,E(): 2.2e-64, (69.9% identity in 249 aa overlap); Q9XD83|PCAI from Streptomyces sp. 2065 (251 aa), FASTA scores: opt: 1121, E(): 8.1e-64, (69.5% identity in 249 aa overlap); etc. Belongs to the 3-oxoacid CoA-transferase subunit A family.; Probable succinyl-CoA:3-ketoacid-coenzyme A transferase (alpha subunit) ScoA (3-oxo acid:CoA transferase) (OXCT A) (succinyl-CoA:3-oxoacid-coenzyme A transferase) 2819870 scoA 888503 scoA Mycobacterium tuberculosis H37Rv Probable succinyl-CoA:3-ketoacid-coenzyme A transferase (alpha subunit) ScoA (3-oxo acid:CoA transferase) (OXCT A) (succinyl-CoA:3-oxoacid-coenzyme A transferase) NP_217020.1 2819124 R 83332 CDS NP_217021.1 15609642 887774 complement(2819953..2821596) 1 NC_000962.3 Rv2505c, (MTCY07A7.11c), len: 547 aa. Probable fadD35, fatty-acid-CoA synthetase, highly similar to many e.g. Q9Z5A6|SC2G5.17 from Streptomyces coelicolor (541 aa),FASTA scores: opt: 2202, E(): 8e-131, (61.55% identity in 528 aa overlap); Q9F9U4|FADD from Pseudomonas stutzeri (Pseudomonas perfectomarina), FASTA scores: opt: 1551, E(): 7.3e-90, (55.55% identity in 551 aa overlap); Q987S7|MLR6932 from Rhizobium loti (Mesorhizobium loti) (590 aa), FASTA scores: opt: 1453, E(): 1.1e-83, (50.7% identity in 564 aa overlap); etc.; Probable fatty-acid-CoA ligase FadD35 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 2821596 fadD35 887774 fadD35 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD35 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_217021.1 2819953 R 83332 CDS NP_217022.1 15609643 888516 2821712..2822359 1 NC_000962.3 Rv2506, (MTCY07A7.12), len: 215 aa. Probable transcriptional regulator, TetR family, similar to many others e.g. Q9L078|SCC105.06c putative TetR-family regulatory protein from Streptomyces coelicolor (208 aa),FASTA scores: opt: 333, E(): 1.5e-14, (48.75% identity in 197 aa overlap); Q9X7X6|SC6A5.30c putative regulatory protein from Streptomyces coelicolor (404 aa), FASTA scores: opt: 267, E(): 4.8e-10, (30.45% identity in 207 aa overlap) (similarity only with C-terminus for this one); Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa), FASTA scores: opt: 239, E(): 1.8e-08, (29.9% identity in 184 aa overlap); etc. Also similar to transcriptional regulatory proteins from Mycobacterium tuberculosis e.g. O05858|Rv3208|MTCY07D11.18c (228 aa), FASTA scores: opt: 218, E(): 4.4e-07, (30.35% identity in 191 aa overlap); C-terminus of P95251|Rv1963c|MTV051.01c|MTCY09F9.01 (406 aa), FASTA scores: opt: 238, E(): 3.6e-08, (28.25% identity in 177 aa overlap); P96839|Rv3557c|MTCY06G11.04c (200 aa),FASTA scores: opt: 215, E(): 6.2e-07, (38.25% identity in 148 aa overlap); etc. Equivalent to AAK46885 from Mycobacterium tuberculosis strain CDC1551 (231 aa) but shorter 16 aa. Contains probable helix-turn-helix motif at aa 46-67, (Score 1660, +4.84 SD). Belongs to the TetR/AcrR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably TetR-family) 2822359 888516 Rv2506 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR-family) NP_217022.1 2821712 D 83332 CDS NP_217023.1 15609644 888244 2822438..2823259 1 NC_000962.3 Rv2507, (MTCY07A7.13), len: 273 aa. Possible conserved pro-rich membrane protein (N-terminal half is Proline-rich), highly similar to Q9CCU3|ML0431 putative membrane protein from Mycobacterium leprae (259 aa) (alias O07711|MLCL383.38c but longer 2 aa), FASTA scores: opt: 968, E(): 1.4e-31, (60.35% identity in 275 aa overlap). Contains potential membrane spanning region. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved proline rich membrane protein 2823259 888244 Rv2507 Mycobacterium tuberculosis H37Rv Possible conserved proline rich membrane protein NP_217023.1 2822438 D 83332 CDS NP_217024.1 15609645 888527 complement(2823256..2824593) 1 NC_000962.3 Rv2508c, (MTCY07A7.14c), len: 445 aa. Probable conserved integral membrane leu-, ala-rich protein,equivalent to Q9CCU4|ML0430 putative membrane protein from Mycobacterium leprae (454 aa) (alias O07710|MLCL383.37 longer 10 aa), FASTA scores: opt: 2205, E(): 2.5e-124,(75.75% identity in 441 aa overlap). Also similar to hypothetical or membrane proteins e.g. BAB50841|MLL4103 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (458 aa), FASTA scores: opt: 396, E(): 2.4e-16,(27.75% identity in 447 aa overlap); Q9RKX9|SC6D7.19c putative integral membrane protein from Streptomyces coelicolor (486 aa), FASTA scores: opt: 323, E(): 5.7e-12,(28.95% identity in 428 aa overlap); P42306|YXIO_BACSU probable integral membrane protein from Bacillus subtilis (428 aa), FASTA scores: opt: 220, E(): 7.2e-06, (20.35% identity in 413 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Q10564|Y876_MYCTU|Rv0876c|MT0899|MTCY31.04c (548 aa), FASTA scores: opt: 184, E(): 0.0012, (24.7% identity in 466 aa overlap).; Probable conserved integral membrane leucine and alanine rich protein 2824593 888527 Rv2508c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane leucine and alanine rich protein NP_217024.1 2823256 R 83332 CDS NP_217025.1 15609646 888526 2824678..2825484 1 NC_000962.3 Rv2509, (MTCY07A7.15), len: 268 aa. Probable ala-rich oxidoreductase, short-chain dehydrogenase/reductase, equivalent to O07709|MLCL383.36c|ML0429 dehydrogenase (putative oxidoreductase) from Mycobacterium leprae (268 aa), FASTA scores: opt: 1509, E(): 2.6e-84, (88.75% identity in 267 aa overlap). Also highly similar to others e.g. O86553|SC1F2.16c putative dehydrogenase from Streptomyces coelicolor (276 aa), FASTA scores: opt: 492, E(): 9.5e-23,(38.15% identity in 262 aa overlap); Q9I5R3|PA0658 probable short-chain dehydrogenase from Pseudomonas aeruginosa (266 aa), FASTA scores: opt: 472, E(): 1.5e-21, (37.8% identity in 246 aa overlap); AAK22120|CC0133 oxidoreductase (short-chain dehydrogenase/reductase family) from Caulobacter crescentus (266 aa), FASTA scores: opt: 428,E(): 6.9e-19, (35.8% identity in 243 aa overlap); etc. Also highly similar or similar to oxidoreductases from Mycobacterium tuberculosis e.g. Q10782|Rv1544|MTCY48.21 putative ketoacyl reductase (267 aa), FASTA scores: opt: 656, E(): 1.1e-32, (43.05% identity in 267 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 2825484 888526 Rv2509 Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_217025.1 2824678 D 83332 CDS NP_217026.1 15609647 888613 complement(2825488..2827089) 1 NC_000962.3 Rv2510c, (MTCY07A7.16c), len: 533 aa. Conserved protein, highly similar, but longer approximately 20 aa, to others e.g. Q9ABY0|CC0090 hypothetical protein from Caulobacter crescentus (516 aa), FASTA scores: opt: 1282,E(): 8.4e-63, (45.1% identity in 490 aa overlap); Q9A130|SPY0500 hypothetical protein from Streptococcus pyogenes (500 aa), FASTA scores: opt: 1281, E(): 9.3e-63,(43.8% identity in 491 aa overlap); Q985L5|MLR7622 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (515 aa), FASTA scores: opt: 1259, E(): 1.5e-61,(44.1% identity in 510 aa overlap); P39342|YJGR_ECOLI|B4263 hypothetical 54.3 KDA protein from Escherichia coli strain K12 (500 aa), FASTA scores: opt: 1257, E(): 1.9e-61, (42.7% identity in 501 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; hypothetical protein 2827089 888613 Rv2510c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217026.1 2825488 R 83332 CDS NP_217027.1 15609648 888633 2827157..2827804 1 NC_000962.3 Rv2511, (MTCY07A7.17), len: 215 aa. Orn,oligoribonuclease, equivalent to O07708|ORN_MYCLE|ORN|ML0427|MLCL383.34c oligoribonuclease from Mycobacterium leprae (215 aa), FASTA scores: opt: 1170, E(): 3.5e-65, (84.5% identity in 213 aa overlap). Also highly similar to many e.g. P57667|ORN_STRGR|ORNA from Streptomyces griseus (201 aa), FASTA scores: opt: 807, E(): 7.7e-43, (59.0% identity in 200 aa overlap); ORN_STRCO|ORNA|2SC13.01 from Streptomyces coelicolor (200 aa), FASTA scores: opt: 799, E(): 2.4e-42, (59.7% identity in 201 aa overlap); P39287|ORN_ECOLI|B4162 from Escherichia coli strain K12 (180 aa), FASTA scores: opt: 519, E(): 3.9e-25, (47.4% identity in 173 aa overlap); etc. Belongs to the oligoribonuclease family.; Oligoribonuclease Orn 2827804 orn 888633 orn Mycobacterium tuberculosis H37Rv Oligoribonuclease Orn NP_217027.1 2827157 D 83332 CDS NP_217028.1 15609649 888515 complement(2828556..2829803) 1 NC_000962.3 Rv2512c, (MTCY07A7.18c), len: 415 aa. Transposase for IS1081, identical to P35882|TRA1_MYCBO transposase for insertion sequence element IS1081 from Mycobacterium bovis (415 aa), FASTA scores: opt: 2680, E(): 1.9e-162, (100.0% identity in 415 aa overlap). Also highly similar to others from Mycobacterium tuberculosis e.g. P96354|Rv1047|MTCY10G2.02c|Rv3115|MTCY164.25|Rv3023c|MTV01 2.38c (415 aa), FASTA scores: opt: 2675, E(): 3.9e-162, (99.75% identity in 415 aa overlap). Contains PS00435 Peroxidases proximal heme-ligand signature, PS01007 Transposases,Mutator family, signature. Belongs to the mutator family of transposase.; Transposase for insertion sequence element IS1081 2829803 888515 Rv2512c Mycobacterium tuberculosis H37Rv Transposase for insertion sequence element IS1081 NP_217028.1 2828556 R 83332 CDS NP_217029.1 15609650 887808 2830161..2830583 1 NC_000962.3 Rv2513, (MTCY07A7.19), len: 140 aa. Hypothetical unknown protein.; Hypothetical protein 2830583 887808 Rv2513 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217029.1 2830161 D 83332 CDS NP_217030.1 15609651 887878 complement(2830877..2831338) 1 NC_000962.3 Rv2514c, (MTCY07A7.20c), len: 153 aa. Conserved hypothetical protein, showing some similarity to Q9PG05|XF0497 hypothetical protein from Xylella fastidiosa (155 aa), FASTA scores: opt: 215, E(): 1.4e-07, (30.6% identity in 160 aa overlap).; hypothetical protein 2831338 887878 Rv2514c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217030.1 2830877 R 83332 CDS NP_217031.1 15609652 887812 complement(2831344..2832591) 1 NC_000962.3 Rv2515c, (MTCY07A7.21c), len: 415 aa. Conserved hypothetical protein, showing some similarity to Q9PG06|XF0496 hypothetical protein from Xylella fastidiosa (391 aa), FASTA scores: opt: 388, E(): 4.4e-18, (27.8% identity in 399 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature.; hypothetical protein 2832591 887812 Rv2515c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217031.1 2831344 R 83332 CDS NP_217032.2 57116997 887186 complement(2832710..2833513) 1 NC_000962.3 Rv2516c, (MTV009.01c), len: 267 aa. Hypothetical unknown protein. Contains probable helix-turn-helix motif at aa 98 to 119 (Score 1743, +5.12 SD). C-terminus extended since first submission (+ 18 aa).; Hypothetical protein 2833513 887186 Rv2516c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217032.2 2832710 R 83332 CDS NP_217033.1 15609654 887673 complement(2833510..2833761) 1 NC_000962.3 Rv2517c, (MTV009.02c), len: 83 aa. Unknown protein. Equivalent to AAK46899 from Mycobacterium tuberculosis strain CDC1551 (97 aa) but shorter 14 aa.; hypothetical protein 2833761 887673 Rv2517c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217033.1 2833510 R 83332 CDS NP_217034.1 15609655 888160 complement(2834109..2835335) 1 NC_000962.3 Rv2518c, (MTV009.03c), len: 408 aa. Probable ldtB,L,D-transpeptidase, highly similar to O07707|MLCL383.3 hypothetical 43.6 KDA protein from Mycobacterium leprae (407 aa), FASTA scores: opt: 2300, E(): 1.2e-130, (82.5% identity in 406 aa overlap); Q9CCU5|LPPS|ML0426 putative secreted protein from Mycobacterium leprae (404 aa), FASTA scores: opt: 2279, E(): 2.3e-129, (82.4% identity in 403 aa overlap); and Q9CB49|ML2446 possible lipoprotein from Mycobacterium leprae (441 aa), FASTA scores: opt: 736, E(): 8.4e-37, (35.6% identity in 399 aa overlap). Also similar to other proteins from several organisms e.g. Q9X811|SC6G10.26c putative secreted protein from Streptomyces coelicolor (424 aa), FASTA scores: opt: 867,E(): 1.1e-44, (32.25% identity in 403 aa overlap); Q9L1E8|SC3D11.14 putative lipoprotein from Streptomyces coelicolor (416 aa), FASTA scores: opt: 737, E(): 7e-37,(32.95% identity in 413 aa overlap); Q9KYV1|SCE22.11 putative lipoprotein from Streptomyces coelicolor (407 aa),FASTA scores: opt: 721, E(): 6.2e-36, (33.5% identity in 400 aa overlap). And similar to several hypothetical mycobacterial proteins e.g. Q11149|Y483_MYCTU|Rv0483|MT0501|MTCY20G9.09 (451 aa), FASTA scores: opt: 763, E(): 2.1e-38, (34.85% identity in 402 aa overlap). Has very long signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as lppS; Probable L,D-transpeptidase LdtB 2835335 ldtB 888160 ldtB Mycobacterium tuberculosis H37Rv Probable L,D-transpeptidase LdtB NP_217034.1 2834109 R 83332 CDS YP_177888.1 57116998 888172 2835785..2837263 1 NC_000962.3 Rv2519, (MTV009.04), len: 492 aa. PE26, Member of the M. tuberculosis PE family (see citation below), highly similar to many e.g. Q50630|YP91_MYCTU|Rv2591|MT2668.1|MTCY227.10c (543 aa),FASTA scores: opt: 848, E(): 3e-30, (39.55% identity in 445 aa overlap).; PE family protein PE26 2837263 PE26 888172 PE26 Mycobacterium tuberculosis H37Rv PE family protein PE26 YP_177888.1 2835785 D 83332 CDS NP_217036.1 15609657 887804 complement(2837388..2837615) 1 NC_000962.3 Rv2520c, (MTV009.05c), len: 75 aa. Possible conserved membrane protein, equivalent to O07706|MLCL383.32 hypothetical 10.0 KDA protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 290, E(): 4.1e-14, (58.65% identity in 75 aa overlap); and Q9CCU6|ML0425 putative membrane protein from Mycobacterium leprae (75 aa), FASTA scores: opt: 286, E(): 6.6e-14, (57.35% identity in 75 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved membrane protein 2837615 887804 Rv2520c Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_217036.1 2837388 R 83332 CDS NP_217037.1 15609658 887694 2837684..2838157 1 NC_000962.3 Rv2521, (MTV009.06), len: 157 aa. Probable bcp,bacterioferritin comigratory protein, equivalent to O07705|BCP|ML0424 from Mycobacterium leprae (161 aa), FASTA scores: opt: 829, E(): 6.8e-46, (79.6% identity in 157 aa overlap). Also highly similar to Q9KZQ2|SCE6.38 hypothetical 16.8 KDA protein Streptomyces coelicolor (155 aa), FASTA scores: opt: 727, E(): 2e-39, (69.5% identity in 154 aa overlap); P23480|AAG57590|BCP_ECOLI|B2480|BAB36765|Z3739|ECS3342 bacterioferritin comigratory protein from Escherichia coli strain K12 (156 aa), FASTA scores: opt: 513, E(): 8.3e-26,(48.3% identity in 149 aa overlap); Q9RW23|DR0846 bacterioferritin comigratory protein from Deinococcus radiodurans (175 aa), FASTA scores: opt: 465, E(): 1e-22,(46.5% identity in 157 aa overlap); P44411|BCP_HAEIN|HI0254 bacterioferritin comigratory protein from Haemophilus influenzae (155 aa), FASTA scores: opt: 453, E(): 5.3e-22,(47.5% identity in 139 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv1608c|MTV046.06|bcpB and Rv2238c|MTCY427.19c|hpE.; Probable bacterioferritin comigratory protein Bcp 2838157 bcp 887694 bcp Mycobacterium tuberculosis H37Rv Probable bacterioferritin comigratory protein Bcp NP_217037.1 2837684 D 83332 CDS NP_217038.1 15609659 887375 complement(2838129..2839541) 1 NC_000962.3 Rv2522c, (MTV009.07c), len: 470 aa. Conserved hypothetical protein, equivalent, but longer 20 aa, to Q9X7E4|ML1193|MLCB458.08 from hypothetical 46.6 KDA protein Mycobacterium leprae (442 aa), FASTA scores: opt: 2521,E(): 4.1e-142, (86.35% identity in 440 aa overlap). Also similar to various proteins e.g. Q9K425|SCG22.20 putative peptidase from Streptomyces coelicolor (451 aa), FASTA scores: opt: 1097, E(): 1.1e-57, (42.5% identity in 451 aa overlap); Q9FCK3|2SC3B6.09 putative peptidase from Streptomyces coelicolor (470 aa), FASTA scores: opt: 669,E(): 2.8e-32, (34.2% identity in 462 aa overlap); Q98AF9|MLL6018 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (486 aa), FASTA scores: opt: 622, E(): 1.7e-29, (33.95% identity in 442 aa overlap); Q9RSU7|DR2025 ARGE/DAPE/ACY1 family protein from Deinococcus radiodurans (459 aa), FASTA scores: opt: 616, E(): 3.7e-29, (34.15% identity in 442 aa overlap); etc (include some similarity to hypothetical proteins from C. elegans and yeast). Alternative start possible at 6687 but then no RBS obvious.; hypothetical protein 2839541 887375 Rv2522c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217038.1 2838129 R 83332 CDS NP_217039.1 15609660 888626 complement(2839538..2839930) 1 NC_000962.3 Rv2523c, (MT2599, MTV009.08c), len: 130 aa. AcpS,holo-[Acyl Carrier Protein] synthase (see citation below),equivalent to Q9X7E3|ACPS_MYCLE|ML1192|MLCB458.07 holo-[acyl-carrier protein] synthase from Mycobacterium leprae (130 aa), FASTA scores: opt: 732, E(): 5.5e-42,(87.5% identity in 128 aa overlap). Also similar to others e.g. O86785|ACPS_STRCO|SC6G4.22c from Streptomyces coelicolor (123 aa), FASTA scores: opt: 204, E(): 6.6e-07,(36.7% identity in 139 aa overlap); Q9KPB6|VC2457 from Vibrio cholerae (126 aa), FASTA scores: opt: 163, E(): 0.00036, (32.55% identity in 129 aa overlap); P24224|ACPS_ECOLI|DPJ|B2563 from Escherichia coli strain K12 (125 aa), FASTA scores: opt: 151, E(): 0.0022, (30.55% identity in 131 aa overlap); etc. Belongs to the ACPS family. Acts on fas-I enzymes in C. glutamicum (See Chalut et al., 2006).; holo-[acyl-carrier protein] synthase AcpS (holo-ACP synthase) (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) 2839930 acpS 888626 acpS Mycobacterium tuberculosis H37Rv holo-[acyl-carrier protein] synthase AcpS (holo-ACP synthase) (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) NP_217039.1 2839538 R 83332 CDS NP_217040.1 15609661 887704 complement(2840123..2849332) 1 NC_000962.3 Rv2524c, (MTCY159.32, MTV009.09c), len: 3069 aa. Probable fas, Fatty Acid Synthase, equivalent to Q9X7E2|fas|ML1191 putative type I fatty acid synthase from Mycobacterium leprae (3076 aa), FASTA scores: opt: 17484,E(): 0, (85.8% identity in 3081 aa overlap). Also similar to others e.g. Q04846|fas|Q59497 from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (3104 aa), FASTA scores: opt: 3981, E(): 5.5e-203, (49.8% identity in 3099 aa overlap); Q48926|fas from Mycobacterium bovis (2796 aa),FASTA scores: opt: 2098, E(): 3.9e-103, (59.7% identity in 2862 aa overlap) (see Fernandes et al., 1996); P34731|FAS1_CANAL fatty acid synthase subunit beta from Candida albicans (Yeast) (2037 aa), FASTA scores: opt: 955,E(): 1.3e-42, (27.4% identity in 1926 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00606 Beta-ketoacyl synthases active site.; Probable fatty acid synthase Fas (fatty acid synthetase) 2849332 fas 887704 fas Mycobacterium tuberculosis H37Rv Probable fatty acid synthase Fas (fatty acid synthetase) NP_217040.1 2840123 R 83332 CDS NP_217041.1 15609662 888612 complement(2849852..2850574) 1 NC_000962.3 Rv2525c, (MTCY159.31), len: 240 aa. Conserved hypothetical protein, equivalent to Q9X7E1|ML1190|MLCB458.05 hypothetical 25.3 KDA protein from Mycobacterium leprae (239 aa), FASTA scores: opt: 1358,E(): 1e-75, (82.15% identity in 241 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Conserved hypothetical protein Secreted; predicted to be a substrate of the twin arginine translocation (tat) export system 2850574 888612 Rv2525c Mycobacterium tuberculosis H37Rv Conserved hypothetical protein Secreted; predicted to be a substrate of the twin arginine translocation (tat) export system NP_217041.1 2849852 R 83332 CDS NP_217042.1 15609663 888254 2851091..2851318 1 NC_000962.3 Rv2526, (MTCY159.30c), len: 75 aa. Possible vapB17,antitoxin, part of toxin-antitoxin (TA) operon with Rv2527 (See Arcus et al., 2005; Pandey and Gerdes, 2005).; Possible antitoxin VapB17 2851318 vapB17 888254 vapB17 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB17 NP_217042.1 2851091 D 83332 CDS NP_217043.1 15609664 887266 2851315..2851716 1 NC_000962.3 Rv2527, (MTCY159.29c), len: 133 aa. Possible vapC17,toxin, part of toxin-antitoxin (TA) operon with Rv2526,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. P95007|MTCY159.10c|Rv2546 (137 aa), FASTA scores: opt: 206, E(): 1.4e-07, (38.0% identity in 100 aa overlap); O33299|MTV002.22c|Rv2757c (138 aa), FASTA scores: opt: 201, E(): 3.1e-07, (35.7% identity in 126 aa overlap); and P96411|MTCY08D5.24c|Rv0229c (226 aa), FASTA scores: opt: 153, E(): 0.0011, (32.8% identity in 128 aa overlap).; Possible toxin VapC17 2851716 vapC17 887266 vapC17 Mycobacterium tuberculosis H37Rv Possible toxin VapC17 NP_217043.1 2851315 D 83332 CDS NP_217044.1 15609665 887150 complement(2851751..2852671) 1 NC_000962.3 Rv2528c, (MTCY159.28), len: 306 aa. Probable mrr,restriction system protein, similar to other mrr proteins e.g. Q9RWS8|DR0587|MRR from Deinococcus radiodurans (306 aa), FASTA scores: opt: 776, E(): 4.2e-40, (40.45% identity in 309 aa overlap); P24202|MRR_ECOLI|B4351 from Escherichia coli strain K12 (304 aa), FASTA scores: opt: 647, E(): 2.9e-32, (35.25% identity in 309 aa overlap); Q9RX07|DR0508 from Deinococcus radiodurans (336 aa), FASTA scores: opt: 456, E(): 1.3e-20, (37.3% identity in 319 aa overlap); etc.; Probable restriction system protein Mrr 2852671 mrr 887150 mrr Mycobacterium tuberculosis H37Rv Probable restriction system protein Mrr NP_217044.1 2851751 R 83332 CDS NP_217045.1 15609666 887153 2852875..2854266 1 NC_000962.3 Rv2529, (MTCY159.27c), len: 463 aa. Hypothetical unknown protein. Note that C-terminal part is similar to short region of Q53609|MTS1_STRAL|SALIM modification methylase SALI from Streptomyces albus G (587 aa), FASTA scores: opt: 170, E(): 0.016, (59.45% identity in 37 aa overlap).; Hypothetical protein 2854266 887153 Rv2529 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217045.1 2852875 D 83332 CDS NP_217046.1 15609667 887192 complement(2854267..2854686) 1 NC_000962.3 Rv2530c, (MTCY159.26), len: 139 aa. Possible vapC39,toxin, part of toxin-antitoxin (TA) operon with Rv2530A,contains PIN domain, see Arcus et al. 2005. Highly similar to others in Mycobacterium tuberculosis e.g. O53219|Rv2494|MTV008.50 (141 aa), FASTA scores: opt: 380,E(): 3.6e-19, (48.0% identity in 125 aa overlap); and O53372|Rv3320c|MTV016.20c (142 aa), FASTA scores: opt: 286,E(): 9.3e-13, (41.35% identity in 133 aa overlap); and similar to others e.g. O07760|Rv0617|MTCY19H5.04c (133 aa),FASTA scores: opt: 158, E(): 0.00048, (39.55% identity in 129 aa overlap). Also some similarity with CAC48798|SMB20412 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (54 aa), FASTA scores: opt: 184, E(): 3.7e-06, (53.85% identity in 52 aa overlap); and CAC48797|SMB20411 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (82 aa), FASTA scores: opt: 170,E(): 4.8e-05, (44.45% identity in 63 aa overlap).; Possible toxin VapC39 Contains PIN domain 2854686 vapC39 887192 vapC39 Mycobacterium tuberculosis H37Rv Possible toxin VapC39 Contains PIN domain NP_217046.1 2854267 R 83332 CDS YP_177672.1 57116999 3205085 complement(2854683..2854907) 1 NC_000962.3 Rv2530A, len: 74 aa. Possible vapB39, antitoxin,part of toxin-antitoxin (TA) operon with Rv2530c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O53218|Rv2493 (73 aa), FASTA scores: opt: 240, E(): 5.7e-11, (56.75% identity in 74 aa overlap); and Q92WE1|RB0399|SMB20413 hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti)p lasmid pSymB (megaplasmid 2) (75 aa), FASTA scores: opt: 226, E(): 6.5e-10, (56.00% identity in 75 aa overlap).; Possible antitoxin VapB39 2854907 vapB39 3205085 vapB39 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB39 YP_177672.1 2854683 R 83332 CDS YP_177889.1 57117000 887216 complement(2854938..2857781) 1 NC_000962.3 Rv2531c, (MTCY159.25), len: 947 aa. Probable amino acid decarboxylase, equivalent to Q9CCR8|adi|ML0524 putative amino acid decarboxylase from Mycobacterium leprae (950 aa), FASTA scores: opt: 5426, E(): 0, (86.45% identity in 951 aa overlap). Also similar to other amino acid decarboxylases (but longer in N-terminus) e.g. Q9I2S7|PA1818 probable ORN/ARG/LYS amino acid decarboxylase from Pseudomonas aeruginosa (751 aa), FASTA scores: opt: 434, E(): 2.5e-19, (29.15% identity in 738 aa overlap); Q9CML3|SPEF|PM0806 ornithine decarboxylase from Pasteurella multocida (720 aa), FASTA scores: opt: 402, E(): 2.4e-17,(24.85% identity in 752 aa overlap); P21169|DCOR_ECOLI|spec|B2965|BAB37264|ECS3841|AAG58096 ornithine decarboxylase isozyme (constitutive enzyme) from Escherichia coli strain K12 (711 aa), FASTA scores: opt: 396, E(): 5.6e-17, (28.0% identity in 646 aa overlap); P44317|DCOR_HAEIN|SPEF|HI0591 ornithine decarboxylase from Haemophilus influenzae (720 aa), FASTA scores: opt: 393,E(): 8.8e-17, (25.05% identity in 743 aa overlap) ; etc. Seems to belong to family 1 of ornithine, lysine, and arginine decarboxylases. Note that previously known as adi.; Probable amino acid decarboxylase 2857781 887216 Rv2531c Mycobacterium tuberculosis H37Rv Probable amino acid decarboxylase YP_177889.1 2854938 R 83332 CDS NP_217048.1 15609669 887152 complement(2857853..2858254) 1 NC_000962.3 Rv2532c, (MTCY159.24), len: 133 aa. Hypothetical unknown protein, equivalent to AAK46918 from Mycobacterium tuberculosis strain CDC1551 but shorter 157 aa.; Hypothetical protein 2858254 887152 Rv2532c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217048.1 2857853 R 83332 CDS NP_217049.1 15609670 887359 complement(2858254..2858724) 1 NC_000962.3 Rv2533c, (MT2608, MTCY159.23), len: 156 aa. NusB, N utilization substance protein (see citations below),equivalent to Q9CCR9|NUSB_MYCLE|ML0523 N utilization substance protein B from Mycobacterium leprae (190 aa),FASTA scores: opt: 749, E(): 2.6e-41, (75.7% identity in 148 aa overlap). Also highly similar to others e.g. Q9KXR0|SC9C5.14 from Streptomyces coelicolor (142 aa),FASTA scores: opt: 358, E(): 2.7e-16, (45.0% identity in 140 aa overlap); P54520|NUSB_BACSU from Bacillus subtilis (131 aa), FASTA scores: opt: 315, E(): 1.5e-13, (39.55% identity in 129 aa overlap); O83979|NUSB_TREPA|TP1015 from Treponema pallidum (141 aa), FASTA scores: opt: 268, E(): 1.6e-10, (36.95% identity in 138 aa overlap); etc. Belongs to the NusB family.; N utilization substance protein NusB (NusB protein) 2858724 nusB 887359 nusB Mycobacterium tuberculosis H37Rv N utilization substance protein NusB (NusB protein) NP_217049.1 2858254 R 83332 CDS NP_217050.1 15609671 888437 complement(2858727..2859290) 1 NC_000962.3 Rv2534c, (MTCY159.22), len: 187 aa. Probable efp,elongation factor P, equivalent to Q9CCS0|EFP|ML0522 elongation factor P from Mycobacterium leprae (187 aa),FASTA scores: opt: 1158, E(): 2.1e-67, (94.1% identity in 186 aa overlap). Also highly similar to many e.g. Q45288|EFP_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (187 aa), FASTA scores: opt: 843,E(): 3.4e-47, (69.5% identity in 187 aa overlap); Q9KXQ9|EFP from Streptomyces coelicolor (188 aa), FASTA scores: opt: 833, E(): 1.5e-46, (67.0% identity in 188 aa overlap); P49778|EFP_BACSU from Bacillus subtilis (185 aa),FASTA scores: opt: 607, E(): 4.6e-32, (47.8% identity in 182 aa overlap); P33398|EFP_ECOLI|B4147 from Escherichia coli strain K12 (187 aa), FASTA scores: opt: 503, E(): 1.8e-27, (42.3% identity in 182 aa overlap); etc. Belongs to the elongation factor P family.; Probable elongation factor P Efp 2859290 efp 888437 efp Mycobacterium tuberculosis H37Rv Probable elongation factor P Efp NP_217050.1 2858727 R 83332 CDS NP_217051.1 15609672 888409 complement(2859300..2860418) 1 NC_000962.3 Rv2535c, (MTCY159.21), len: 372 aa. Probable pepQ,cytoplasmic peptidase, equivalent to Q9CCS1|PEPQ|ML0521 putative cytoplasmic peptidase from Mycobacterium leprae (376 aa), FASTA scores: opt: 1954, E(): 1.1e-105, (82.7% identity in 376 aa overlap). Also similar to other peptidases e.g. P54518|YQHT_BACSU putative peptidase (belongs to peptidase family M24B) from Bacillus subtilis (353 aa), FASTA scores: opt: 808, E(): 1.6e-39, (39.65% identity in 368 aa overlap); Q9KXQ8|SC9C5.16c putative peptidase from Streptomyces coelicolor (368 aa), FASTA scores: opt: 803, E(): 3.2e-39, (43.15% identity in 380 aa overlap); Q9K950|BH2800 XAA-pro dipeptidase from Bacillus halodurans (355 aa), FASTA scores: opt: 801, E(): 4.1e-39,(39.45% identity in 365 aa overlap); etc. Note that second part of protein is similar to second part of MTCY49.29c|Rv2089c|MT2150|MTCY49.29c probable dipeptidase; belongs to peptidase family M24B from Mycobacterium tuberculosis (375 aa) (33.9% identity in 354 aa overlap) blast results: Score: 142 bits (359), E: 4e-33, Identities: 86/224 (38%), Positives: 119/224 (52%), Gaps: 4/224 (1%). Could be belong to peptidase family M24B. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable cytoplasmic peptidase PepQ 2860418 pepQ 888409 pepQ Mycobacterium tuberculosis H37Rv Probable cytoplasmic peptidase PepQ NP_217051.1 2859300 R 83332 CDS NP_217052.1 15609673 888386 2860452..2861144 1 NC_000962.3 Rv2536, (MTCY159.20c), len: 230 aa. Probable conserved transmembrane protein, equivalent to Q9CCS2|ML0520 putative membrane protein from Mycobacterium leprae (202 aa), FASTA scores: opt: 812, E(): 2e-41, (63.2% identity in 201 aa overlap). Also similar in part to Q9HMD5|VNG2594c from Halobacterium sp. strain NRC-1 (117 aa), FASTA scores: opt: 33.6, E(): 1.8, (33.6% identity in 116 aa overlap); and perhaps AAK65752|SMA1996 putative ABC transporter permease protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (323 aa), FASTA scores: opt: 117, E(): 6.1, (30.6% identity in 121 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 2861144 888386 Rv2536 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217052.1 2860452 D 83332 CDS NP_217053.1 15609674 888397 complement(2861148..2861591) 1 NC_000962.3 Rv2537c, (MTCY159.19), len: 147 aa. AroD (alternate gene name: aroQ), 3-dehydroquinate dehydratase (see citation below), equivalent to Q9CCS3|AROD|ML0519 3-dehydroquinate dehydratase from Mycobacterium leprae (145 aa), FASTA scores: opt: 803, E(): 3.4e-46, (85.9% identity in 142 aa overlap). Also highly similar to many e.g. P96750|AROQ_CORPS from Corynebacterium pseudotuberculosis (146 aa), FASTA scores: opt: 559, E(): 4.1e-30, (61.05% identity in 136 aa overlap); Q9K949|BH2801 from Bacillus halodurans (145 aa), FASTA scores: opt: 453, E(): 4e-23,(52.15% identity in 138 aa overlap); P54517|AROQ_BACSU|YQHS from Bacillus subtilis (148 aa), FASTA scores: opt: 419,E(): 7.1e-21, (45.3% identity in 139 aa overlap); etc. Contains PS01029 Dehydroquinase class II signature. Belongs to the type-II 3-dehydroquinase family.; 3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase) 2861591 aroD 888397 aroD Mycobacterium tuberculosis H37Rv 3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase) NP_217053.1 2861148 R 83332 CDS NP_217054.1 15609675 888392 complement(2861588..2862676) 1 NC_000962.3 Rv2538c, (MTCY159.18), len: 362 aa. AroB,3-dehydroquinate synthase (see citations below), equivalent to Q9CCS4|AROB_MYCLE|ML0518 3-dehydroquinate synthase from Mycobacterium leprae (361 aa), FASTA scores: opt: 2059,E(): 3.3e-117, (87.25% identity in 361 aa overlap). Also highly similar to many e.g. Q9KXQ6|AROB from Streptomyces coelicolor (363 aa), FASTA scores: opt: 1363, E(): 4e-75,(60.05% identity in 358 aa overlap); Q9X5D2|AROB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (366 aa), FASTA scores: opt: 1154, E(): 1.7e-62, (50.95% identity in 359 aa overlap); P07639|AROB_ECOLI|B3389 from Escherichia coli strain K12 (362 aa), FASTA scores: opt: 771, E(): 2.4e-39, (40.6% identity in 345 aa overlap); etc. Belongs to the dehydroquinate synthase family.; 3-dehydroquinate synthase AroB 2862676 aroB 888392 aroB Mycobacterium tuberculosis H37Rv 3-dehydroquinate synthase AroB NP_217054.1 2861588 R 83332 CDS NP_217055.1 15609676 887434 complement(2862673..2863203) 1 NC_000962.3 Rv2539c, (MTCY159.17), len: 176 aa. AroK, shikimate kinase (see citations below), equivalent to Q9CCS5|AROK|ML0517 putative shikimate kinase from Mycobacterium leprae (199 aa), FASTA scores: opt: 852, E(): 1.3e-42, (79.65% identity in 167 aa overlap). Also highly similar to many e.g. Q9X5D1|AROK_CORG from Corynebacterium glutamicum (Brevibacterium flavum) (169 aa), FASTA scores: opt: 478, E(): 5.4e-21, (47.0% identity in 168 aa overlap); Q9KXQ5|AROK from Streptomyces coelicolor (171 aa), FASTA scores: opt: 465, E(): 3.1e-20, (49.1% identity in 167 aa overlap); P24167|AROK_ECOLI from Escherichia coli strain K12 (172 aa), FASTA scores: opt: 316, E(): 1.3e-11, (38.4% identity in 164 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A, and PS01128 Shikimate kinase signature. Belongs to the shikimate kinase family.; Shikimate kinase AroK (SK) 2863203 aroK 887434 aroK Mycobacterium tuberculosis H37Rv Shikimate kinase AroK (SK) NP_217055.1 2862673 R 83332 CDS NP_217056.1 15609677 887379 complement(2863207..2864412) 1 NC_000962.3 Rv2540c, (MTCY159.16), len: 401 aa. Probable aroF (alternate gene name: aroC), chorismate synthase,equivalent to Q9CCS6|AROF|ML0516 putative chorismate synthase from Mycobacterium leprae (407 aa), FASTA scores: opt: 2278, E(): 6.2e-123, (88.05% identity in 401 aa overlap). Also highly similar to many e.g. Q9X5D0|AROC_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (410 aa), FASTA scores: opt: 1811,E(): 3e-96, (70.3% identity in 397 aa overlap); Q9KXQ4|AROC_STRCO|AROF|SC9C5.20c from Streptomyces coelicolor (394 aa), FASTA scores: opt: 1710, E(): 1.7e-90,(67.0% identity in 385 aa overlap); Q9KCB7|AROC_BACHD|AROF|BH1656 from Bacillus halodurans (390 aa), FASTA scores: opt: 1196, E(): 3.9e-61, (48.7% identity in 386 aa overlap); etc. Contains PS00788 Chorismate synthase signature 2. Belongs to the chorismate synthase family. Cofactor: reduced flavin, NADH; Probable chorismate synthase AroF (5-enolpyruvylshikimate-3-phosphate phospholyase) 2864412 aroF 887379 aroF Mycobacterium tuberculosis H37Rv Probable chorismate synthase AroF (5-enolpyruvylshikimate-3-phosphate phospholyase) NP_217056.1 2863207 R 83332 CDS NP_217057.1 15609678 887831 2864427..2864834 1 NC_000962.3 Rv2541, (MTCY159.15c), len: 135 aa. Hypothetical unknown ala-rich protein, equivalent to AAK46926|MT2615.1 hypothetical 38.9 KDA protein from Mycobacterium tuberculosis strain CDC1551 but AAK46926|MT2615.1 longer at C-terminus. Questionable ORF. Some similarity with Rv2077A from Mycobacterium tuberculosis (99 aa).; Hypothetical alanine rich protein 2864834 887831 Rv2541 Mycobacterium tuberculosis H37Rv Hypothetical alanine rich protein NP_217057.1 2864427 D 83332 CDS NP_217058.1 15609679 887261 2865130..2866341 1 NC_000962.3 Rv2542, (MTCY159.14c), len: 403 aa. Conserved hypothetical protein, highly similar to AAK46927|MT2616 hypothetical 28.0 KDA protein from Mycobacterium tuberculosis strain CDC1551 (265 aa), FASTA scores: opt: 1776, E(): 2.3e-94, (99.25% identity in 265 aa overlap). And similar to several hypothetical proteins from Mycobacterium tuberculosis (strain H37Rv and CDC1551) e.g. P71654|Rv2797c|MTCY16B7.46 (562 aa), FASTA scores: opt: 537, E(): 2.6e-23, (40.75% identity in 292 aa overlap); P71547|Y963_MYCTU|Rv0963c|MT0992|MTCY10D7.11 (266 aa),FASTA scores: opt: 357, E(): 2.6e-13, (34.6% identity in 234 aa overlap); Q10685|YK77_MYCTU|Rv2077c|MT2137|MTCY49.16c (323 aa), FASTA scores: opt: 261, E(): 9.5e-08, (32.7% identity in 211 aa overlap); etc. Also similar to Q9RDQ9|SC4A7.03 putative secreted protein from Streptomyces coelicolor (406 aa),FASTA scores: opt: 247, E(): 7.3e-07, (30.35% identity in 303 aa overlap).; hypothetical protein 2866341 887261 Rv2542 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217058.1 2865130 D 83332 CDS NP_217059.1 15609680 888052 2866468..2867127 1 NC_000962.3 Rv2543, (MTCY159.13c), len: 219 aa. Probable lppA,conserved lipoprotein, highly similar to upstream ORF P95009|LPPB|Rv2544|MTCY159.12 putative lipoprotein LPPB from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 1240, E(): 1.1e-73, (87.15% identity in 218 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LppA 2867127 lppA 888052 lppA Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppA NP_217059.1 2866468 D 83332 CDS NP_217060.1 15609681 888054 2867124..2867786 1 NC_000962.3 Rv2544, (MTCY159.12c), len: 220 aa. Probable lppB,conserved lipoprotein, highly similar to downstream ORF P95010|MTCY159.13c|LPPA|Rv2543|MTCY159.13 putative lipoprotein LPPA from Mycobacterium tuberculosis (219 aa),FASTA scores: opt: 1242, E(): 4.8e-72, (87.15% identity in 218 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LppB 2867786 lppB 888054 lppB Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppB NP_217060.1 2867124 D 83332 CDS NP_217061.1 15609682 888038 2867783..2868061 1 NC_000962.3 Rv2545, (MTY159.11c), len: 92 aa. Possible vapB18,antitoxin, part of toxin-antitoxin (TA) operon with Rv2546 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. O33300|Rv2758c|MTV002.23c (88 aa), FASTA scores: opt: 151,E(): 9.8e-05, (66.65% identity in 45 aa overlap); and Q10771|Rv1560|MT1611|MTCY48.05 (72 aa), FASTA scores: opt: 84, E(): 8.2, (46.5% identity in 43 aa overlap).; Possible antitoxin VapB18 2868061 vapB18 888038 vapB18 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB18 NP_217061.1 2867783 D 83332 CDS NP_217062.1 15609683 887365 2868154..2868567 1 NC_000962.3 Rv2546, (MTCY159.10c), len: 137 aa. Possible vapC18,toxin, part of toxin-antitoxin (TA) operon with Rv2545,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. P96411|Rv0229c|MTCY08D5.24c (226 aa),FASTA scores: opt: 272, E(): 1.3e-11, (39.7% identity in 136 aa overlap); O33299|Rv2757c|MTV002.22c (138 aa), FASTA scores: opt: 265, E(): 2.5e-11, (38.5% identity in 135 aa overlap); P95026|Rv2527|MTCY159.29c (133 aa), FASTA scores: opt: 206, E(): 2.6e-07, (38.0% identity in 100 aa overlap); etc.; Possible toxin VapC18 2868567 vapC18 887365 vapC18 Mycobacterium tuberculosis H37Rv Possible toxin VapC18 NP_217062.1 2868154 D 83332 CDS NP_217063.1 15609684 888452 2868606..2868863 1 NC_000962.3 Rv2547, (MTCY159.09c), len: 85 aa. Possible vapB19,antitoxin, part of toxin-antitoxin (TA) operon with Rv2548 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. P71666|YD98_MYCTU|Rv1398c|MT1442|MTCY21B4.15c hypothetical 9.4 KDA protein from (85 aa), FASTA scores: opt: 108, E(): 0.33, (37.1% identity in 62 aa overlap); and to CAC45864|SMC01933 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (71 aa), FASTA scores: opt: 105, E(): 0.46, (28.4% identity in 74 aa overlap); Q97W38|SSO10342 hypothetical protein from Sulfolobus solfataricus (58 aa), FASTA scores: opt: 94,E(): 2.3, (46.95% identity in 49 aa overlap).; Possible antitoxin VapB19 2868863 vapB19 888452 vapB19 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB19 NP_217063.1 2868606 D 83332 CDS NP_217064.1 15609685 888412 2868860..2869237 1 NC_000962.3 Rv2548, (MTCY159.08c), len: 125 aa. Possible vapC19,toxin, part of toxin-antitoxin (TA) operon with Rv2547,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity to others in Mycobacterium tuberculosis e.g. P71665|Rv1397c|MTCY21B4.14c hypothetical 15.0 KDA protein (133 aa), FASTA scores: opt: 265, E(): 7.1e-12, (42.3% identity in 123 aa overlap); and to Q97WY5|SSO1975 hypothetical protein from Sulfolobus solfataricus (125 aa), FASTA scores: opt: 131, E(): 0.018,(30.0% identity in 110 aa overlap); O52285|YLE hypothetical 14.9 KDA protein from Agrobacterium radiobacter (133 aa),FASTA scores: opt: 128, E(): 0.03, (32.8% identity in 125 aa overlap); etc.; Possible toxin VapC19 2869237 vapC19 888412 vapC19 Mycobacterium tuberculosis H37Rv Possible toxin VapC19 NP_217064.1 2868860 D 83332 CDS YP_004837057.1 345462036 11117811 complement(2869253..2869627) 1 NC_000962.3 Rv2548A, len: 124 aa. Conserved protein.; hypothetical protein 2869627 11117811 Rv2548A Mycobacterium tuberculosis H37Rv hypothetical protein YP_004837057.1 2869253 R 83332 CDS NP_217065.1 15609686 887193 complement(2869727..2870122) 1 NC_000962.3 Rv2549c, (MTCY159.07), len: 131 aa. Possible vapC20,toxin, part of toxin-antitoxin (TA) operon with Rv2550c,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Conserved hypothetical protein, showing some similarity to P73415|SLL1715 from Synechocystis sp. strain PCC 6803 (157 aa), FASTA scores: opt: 167, E(): 4.2e-05,(29.45% identity in 129 aa overlap); Q9HHY6|VNG6166H from Halobacterium sp. plasmid pNRC200 strain NRC-1 (144 aa),FASTA scores: opt: 133, E(): 0.011, (29.6% identity in 125 aa overlap); and Q9HSU3|VNG0072H from Halobacterium sp. strain NRC-1 (144 aa), FASTA scores: opt: 113, E(): 0.29,(25.75% identity in 136 aa overlap).; Possible toxin VapC20 2870122 vapC20 887193 vapC20 Mycobacterium tuberculosis H37Rv Possible toxin VapC20 NP_217065.1 2869727 R 83332 CDS NP_217066.1 15609687 887353 complement(2870119..2870364) 1 NC_000962.3 Rv2550c, (MTCY159.06), len: 81 aa. Possible vapB20,antitoxin, part of toxin-antitoxin (TA) operon with Rv2549c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to others in M. tuberculosis e.g. Rv0581; Possible antitoxin VapB20 2870364 vapB20 887353 vapB20 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB20 NP_217066.1 2870119 R 83332 CDS NP_217067.1 15609688 887855 complement(2870775..2871194) 1 NC_000962.3 Rv2551c, (MTCY159.05), len: 139 aa. Conserved hypothetical protein, similar to the second part of Q9XAP1|SC10A7.34c putative type IV peptidase from Streptomyces coelicolor (259 aa), FASTA scores: opt: 243,E(): 7.4e-08, (40.95% identity in 144 aa overlap). Also some similarity with other proteins e.g. AAK58497|GSPO GSPO protein from Acetobacter diazotrophicus (261 aa), FASTA scores: opt: 152, E(): 0.025, (33.35% identity in 135 aa overlap).; hypothetical protein 2871194 887855 Rv2551c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217067.1 2870775 R 83332 CDS NP_217068.1 15609689 887330 complement(2871206..2872015) 1 NC_000962.3 Rv2552c, (MTCY159.04), len: 269 aa. Probable aroE,shikimate 5-dehydrogenase, equivalent to Q9CCS7|AROE|ML0515 putative shikimate 5-dehydrogenase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1452, E(): 1.8e-77,(81.5% identity in 270 aa overlap). Also highly similar,but longer 101 aa, to Q9KH59|AROE putative shikimate dehydrogenase (fragment) from Mycobacterium marinum (148 aa), FASTA scores: opt: 729, E(): 1.3e-35, (76.35% identity in 148 overlap); Q9F7W3|AROE from Mycobacterium ulcerans (148 aa), FASTA scores: opt: 718, E(): 5.9e-35, (75.7% identity in 148 aa overlap). And also similar to to others e.g. Q9KXQ2|AROE from Streptomyces coelicolor (255 aa),FASTA scores: opt: 572, E(): 2.8e-26, (43.4% identity in 251 aa overlap); Q98DY3|MLR4492 from Rhizobium loti (Mesorhizobium loti) (280 aa), FASTA scores: opt: 385, E(): 2.2e-15, (34.85% identity in 284 aa overlap); P74591|AROE_SYNY3|SLR1559 from Synechocystis sp. strain PCC 6803 (290 aa), FASTA scores: opt: 347, E(): 3.7e-13, (30.9% identity in 275 aa overlap); P15770|AROE_ECOLI|B3281 from Escherichia coli strain K12 (272 aa), FASTA scores: opt: 230, E(): 7.7e-08, (29.5% identity in 251 aa overlap); etc. Belongs to the shikimate dehydrogenase family.; Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase) 2872015 aroE 887330 aroE Mycobacterium tuberculosis H37Rv Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase) NP_217068.1 2871206 R 83332 CDS NP_217069.1 15609690 888228 complement(2872012..2873265) 1 NC_000962.3 Rv2553c, (MTCY159.03), len: 417 aa. Probable conserved membrane protein, equivalent to Q9CCS8|ML0514 putative membrane protein from Mycobacterium leprae (421 aa), FASTA scores: opt: 1955, E(): 1.1e-111, (72.7% identity in 414 aa overlap). Also similar in part to various proteins e.g. Q9L9G6|NOVB NOVB protein (aminodesoxychorismate lyase) from Streptomyces sphaeroides (284 aa), FASTA scores: opt: 451, E(): 2.9e-2, (37.95% identity in 203 aa overlap); Q9EWY3|2SCG38.36 conserved hypothetical protein from Streptomyces coelicolor (253 aa),FASTA scores: opt: 419, E(): 2.3e-18, (39.2% identity in 171 aa overlap); Q9CHT3|YGCC hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (550 aa), FASTA scores: opt: 379, E(): 1.2e-15, (23.0% identity in 417 aa overlap); O25309|HP0587 aminodeoxychorismate lyase (PABC) from Helicobacter pylori (Campylobacter pylori) (329 aa), FASTA scores: opt: 290,E(): 2e-10, (31.65% identity in 180 aa overlap); etc.; Probable conserved membrane protein 2873265 888228 Rv2553c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_217069.1 2872012 R 83332 CDS NP_217070.1 15609691 887490 complement(2873258..2873770) 1 NC_000962.3 Rv2554c, (MTCY159.02), len: 170 aa. Conserved protein, equivalent to Q9CCS9|ML0513 hypothetical protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 701,E(): 2e-34, (72.05% identity in 161 aa overlap). Also highly similar to Q9KXQ0|SC9C5.24c hypothetical 17.7 KDA protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 461, E(): 2.3e-20, (54.65% identity in 150 aa overlap); and similar to other hypothetical proteins e.g. Q9KDE4 from Bacillus halodurans (140 aa), FASTA scores: opt: 291, E(): 1.9e-10, (38.7% identity in 137 aa overlap); P74662|SLL1547 from Synechocystis sp. strain PCC 6803 (152 aa), FASTA scores: opt: 290, (36.55% identity in 145 aa overlap); Q52673|YQGF_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (159 aa), FASTA scores: opt: 246, E(): 8.4e-08, (34.8% identity in 135 aa overlap); etc.; hypothetical protein 2873770 887490 Rv2554c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217070.1 2873258 R 83332 CDS NP_217071.1 15609692 887726 complement(2873771..2876485) 1 NC_000962.3 Rv2555c, (MTCY318.01c-MTCY159.01), len: 904 aa. Probable alaS, alanyl-tRNA synthetase, equivalent to Q9CCT0|alas|ML0512 alanyl-tRNA synthetase from Mycobacterium leprae (908 aa), FASTA scores: opt: 5013,E(): 0, (84.65% identity in 907 aa overlap). Also highly similar to many e.g. Q9KXP9|alas from Streptomyces coelicolor (890 aa), FASTA scores: opt: 2159, E(): 3.8e-118, (53.45% identity in 907 aa overlap); Q9FFC7 Arabidopsis thaliana (Mouse-ear cress) (954 aa), FASTA scores: opt: 1963, E(): 1.1e-106, (41.1% identity in 925 aa overlap); Q9RS27|DR2300 from Deinococcus radiodurans (890 aa), FASTA scores: opt: 1352, E(): 4.1e-71, (38.05% identity in 915 aa overlap); etc. Belongs to class-II aminoacyl-tRNA synthetase family.; Probable alanyl-tRNA synthetase AlaS (alanine--tRNA ligase) (alanine translase) (ALARS) 2876485 alaS 887726 alaS Mycobacterium tuberculosis H37Rv Probable alanyl-tRNA synthetase AlaS (alanine--tRNA ligase) (alanine translase) (ALARS) NP_217071.1 2873771 R 83332 CDS NP_217072.1 15609693 887451 complement(2876576..2876965) 1 NC_000962.3 Rv2556c, (MTCY09C4.12), len: 129 aa. Conserved hypothetical protein, highly similar to others e.g. Q9EWY5|2SCG38.34 conserved hypothetical protein from Streptomyces coelicolor (140 aa), FASTA scores: opt: 488,E(): 8.2e-26, (58.8% identity in 131 aa overlap); Q9L9G4|NOVD NOVD protein from Streptomyces sphaeroides (143 aa), FASTA scores: opt: 474, E(): 7.2e-25, (60.85% identity in 120 aa overlap); Q9X2I5|TM1872 from Thermotoga maritima (132 aa), FASTA scores: opt: 270, E(): 2.7e-11, (39.55% identity in 129 aa overlap); etc.; hypothetical protein 2876965 887451 Rv2556c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217072.1 2876576 R 83332 CDS NP_217073.1 15609694 887865 2877072..2877746 1 NC_000962.3 Rv2557, (MTCY9C4.11c), len: 224 aa. Conserved protein, highly similar to upstream ORF Q50740|MTCY9C4.10c|Rv2558|MT2635 conserved hypothetical protein from Mycobacterium tuberculosis (236 aa), FASTA scores: opt: 1007, E(): 6.9e-60, (69.2% identity in 224 aa overlap); and Mb2587 in Mycobacterium bovis (224 aa).; hypothetical protein 2877746 887865 Rv2557 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217073.1 2877072 D 83332 CDS NP_217074.1 15609695 887298 2877831..2878541 1 NC_000962.3 Rv2558, (MTCY9C4.10c), len: 236 aa. Conserved protein, highly similar to downstream ORF Q50741|MTCY9C4.11c|Rv2557|MT2645 conserved hypothetical protein from Mycobacterium tuberculosis (224 aa), FASTA scores: opt: 1007, E(): 4.7e-59, (69.2% identity in 224 aa overlap); and Mb2588 in Mycobacterium bovis (236 aa).; hypothetical protein 2878541 887298 Rv2558 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217074.1 2877831 D 83332 CDS NP_217075.1 15609696 887368 complement(2878571..2879929) 1 NC_000962.3 Rv2559c, (MTCY9C4.09), len: 452 aa. Conserved hypothetical ala-, leu-, val-rich protein, equivalent to Q9CCT1|ML0510 hypothetical protein from Mycobacterium leprae (473 aa), FASTA scores: opt: 2411, E(): 3.9e-121,(83.4% identity in 452 aa overlap); O69490|O69490 hypothetical 47.1 KDA protein from Mycobacterium leprae (447 aa), FASTA scores: opt: 2406, E(): 6.9e-121, (83.95% identity in 448 aa overlap). Also highly similar to Q9KXP4|SC9C5.30c conserved ATP/GTP binding protein from Streptomyces coelicolor (451 aa), FASTA scores: opt: 1742,E(): 1.5e-85, (64.4% identity in 430 aa overlap); Q9RT67|DR1898 conserved hypothetical protein from Deinococcus radiodurans (434 aa), FASTA scores: opt: 1147,E(): 6.6e-54, (46.0% identity in 415 aa overlap); P45262|YCAJ_HAEIN|HI1590 hypothetical protein from Haemophilus influenzae (446 aa), FASTA scores: opt: 1140,E(): 1.6e-53, (42.5% identity in 428 aa overlap); etc. Also similar to Q50629|MTCY227.09|RUVB|Rv2592c|MT2669|MTCY227.09 holliday junction DNA helicase from Mycobacterium tuberculosis (344 aa), (30.1% identity in 296 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Conserved hypothetical alanine leucine valine rich protein 2879929 887368 Rv2559c Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine leucine valine rich protein NP_217075.1 2878571 R 83332 CDS NP_217076.1 15609697 887363 2880075..2881052 1 NC_000962.3 Rv2560, (MTCY9C4.08c), len: 325 aa. Probable transmembrane protein, pro-, gly-rich protein.; Probable proline and glycine rich transmembrane protein 2881052 887363 Rv2560 Mycobacterium tuberculosis H37Rv Probable proline and glycine rich transmembrane protein NP_217076.1 2880075 D 83332 CDS NP_217077.1 15609698 887164 2881409..2881702 1 NC_000962.3 Rv2561, (MTCY9C4.07c), len: 97 aa. Conserved hypothetical protein, highly similar in part (and longer 33 aa) to upstream ORF AAK46951|RV2562|MT2638|MTCY9C4.06c conserved hypothetical protein from Mycobacterium tuberculosis (212 aa), FASTA scores: opt: 205, E(): 2e-06,(76.1% identity in 46 aa overlap).; hypothetical protein 2881702 887164 Rv2561 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217077.1 2881409 D 83332 CDS NP_217078.1 15609699 887329 2881758..2882147 1 NC_000962.3 Rv2562, (MTCY9C4.06c), len: 129 aa. Conserved hypothetical protein, highly similar, but shorter 83 aa, to downstream ORF AAK46951|RV2561|MT2638|MTCY9C4.07c conserved hypothetical protein from Mycobacterium tuberculosis (97 aa), FASTA scores: opt: 866, E(): 2.2e-54, (100.0% identity in 129 aa overlap).; hypothetical protein 2882147 887329 Rv2562 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217078.1 2881758 D 83332 CDS NP_217079.1 15609700 887516 2882290..2883339 1 NC_000962.3 Rv2563, (MTCY9C4.05c), len: 349 aa. Probable glutamine-transport transmembrane protein ABC transporter (see citation below), highly similar to O53617|Rv0072|MTV030.16 putative ABC-transporter transmembrane subunit from Mycobacterium tuberculosis (349 aa), FASTA scores: opt: 1772, E(): 1.1e-89, (76.2% identity in 349 aa overlap). Also some similarity with various hypothetical proteins e.g. Q9RYN1|DRA0279 hypothetical 37.1 KDA protein from Deinococcus radiodurans (353 aa), FASTA scores: opt: 347, E(): 6.6e-12, (24.35% identity in 357 aa overlap); BAB58522|SAV2360 conserved hypothetical protein from Staphylococcus aureus subsp. aureus Mu50 (351 aa),FASTA scores: opt: 262, E(): 2.9e-07, (19.4% identity in 356 aa overlap); Q9AK94|SC10A9.10c putative ABC transport system transmembrane protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 172, E(): 0.025, (26.85% identity in 387 aa overlap); etc.; Probable glutamine-transport transmembrane protein ABC transporter 2883339 887516 Rv2563 Mycobacterium tuberculosis H37Rv Probable glutamine-transport transmembrane protein ABC transporter NP_217079.1 2882290 D 83332 CDS NP_217080.1 15609701 887898 2883342..2884334 1 NC_000962.3 Rv2564, (MTCY9C4.04c), len: 330 aa. Probable glnQ,glutamine-transport ATP-binding protein ABC transporter (see citation below), highly similar to many e.g. Q9L0J9|SCD40A.12c putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa), FASTA scores: opt: 598, E(): 2.5e-26, (46.35% identity in 218 aa overlap); O54136|SC2E9.11 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 592, E(): 5.1e-26, (46.55% identity in 219 aa overlap); O29244|AF1018 from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 580, E(): 2.4e-25,(42.4% identity in 210 aa overlap); P75831|YBJZ_ECOLI|B0879 from Escherichia coli strain K12 (648 aa), FASTA scores: opt: 555, E(): 1.3e-23, (39.65% identity in 232 aa overlap); etc. Also highly similar to O53618|Rv0073|MTV030.17 ABC-transporter ATP-binding subunit from Mycobacterium tuberculosis (330 aa), FASTA scores: opt: 1782, E(): 4.7e-92, (83.65% identity in 330 aa overlap); etc. Shows some similarity to Q11040|YC81_MYCTU|MTCY50.01|Rv1281c|MT1318 hypothetical ABC transporter ATP-binding protein from Mycobacterium tuberculosis (612 aa) (32.9 % identity in 234 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop),PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable glutamine-transport ATP-binding protein ABC transporter GlnQ 2884334 glnQ 887898 glnQ Mycobacterium tuberculosis H37Rv Probable glutamine-transport ATP-binding protein ABC transporter GlnQ NP_217080.1 2883342 D 83332 CDS NP_217081.1 15609702 887288 2884611..2886362 1 NC_000962.3 Rv2565, (MTCY9C4.03c), len: 583 aa. Conserved protein, similar in part to Q9A6C3|CC2171 hypothetical protein from Caulobacter crescentus (610 aa), FASTA scores: opt: 765, E(): 2.8e-37, (32.15% identity in 575 aa overlap). C-terminus also highly similar to various bacterial proteins e.g. O34731|YLBK_BACSU hypothetical 28.3 KDA protein from Bacillus subtilis (260 aa), FASTA scores: opt: 386, E(): 2.2e-15, (33.05% identity in 245 aa overlap); CAC45997|SMC01003 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (321 aa),FASTA scores: opt: 352, E(): 2.5e-13, (29.65% identity in 280 aa overlap); Q9K9Q8|BH2587 hypothetical protein from Bacillus halodurans (275 aa), FASTA scores: opt: 334, E(): 2.5e-12, (33.7% identity in 175 aa overlap); etc. And shows similarity to C-terminal half of some eukaryotic proteins e.g. Q9R114|NTE neuropathy target esterase homolog from Mus musculus (Mouse) (1327 aa), FASTA scores: opt: 411, E(): 2.7e-16, (24.45% identity in 626 aa overlap); O60859 neuropathy target esterase from Homo sapiens (Human) (1327 aa), FASTA scores: opt: 410, E(): 3.1e-16, (24.1% identity in 627 aa overlap); Q9U969|SWS|CG2212 swiss cheese protein from Drosophila melanogaster (Fruit fly) (1425 aa), FASTA scores: opt: 401, E(): 1.1e-15, (27.75% identity in 544 aa overlap); etc. Also shows strong similarity to C-terminal half of O05884|Z95121|Rv3239c|MTY20B11.14c hypothetical 110.2 KDA protein from Mycobacterium tuberculosis (1048 aa), FASTA scores: opt: 648, E(): 3e-30, (36.55% identity in 572 aa overlap); and O69695|Rv3728|MTV025.076 putative two-domain membrane protein from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 643, E(): 6e-30, (34.3% identity in 595 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 2886362 887288 Rv2565 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217081.1 2884611 D 83332 CDS NP_217082.1 15609703 887737 2886373..2889795 1 NC_000962.3 Rv2566, (MTCY9C4.02c), len: 1140 aa. Long conserved protein, equivalent to O53120|ML2678 or MLCB1913.12 hypothetical protein from Mycobacterium leprae (1000 aa),FASTA scores: opt: 760, E(): 7.1e-38, (50.2% identity in 1128 aa overlap); and middle part equivalent to Q9ZB40 72.2 KDA protein (fragment) from Mycobacterium leprae (644 aa),FASTA scores: opt: 1017, E(): 1.5e-65, (45.65% identity in 655 aa overlap). Also highly similar to Q98HG6|MLL2877 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (1119 aa), FASTA scores: opt: 1413, E(): 3.7e-77,(52.4% identity in 1148 aa overlap); and N-terminus shows similarity with other proteins e.g. Q9HUN8|PA4926 hypothetical protein from Pseudomonas aeruginosa (311 aa),FASTA scores: opt: 278, E(): 3e-09, (29.95% identity in 284 aa overlap); and upstream ORF Q50652|YP69_MYCTU|Rv2569c|MT2645|MTCY227.32 conserved hypothetical protein from Mycobacterium tuberculosis (314 aa), FASTA scores: opt: 252, E(): 1.1e-07, (28.9% identity in 315 aa overlap). Equivalent to AAK46955 from Mycobacterium tuberculosis strain CDC1551 (1156 aa) but shorter 16 aa.; Long conserved protein 2889795 887737 Rv2566 Mycobacterium tuberculosis H37Rv Long conserved protein NP_217082.1 2886373 D 83332 CDS NP_217083.1 15609704 888578 2889795..2892449 1 NC_000962.3 Rv2567, (MTCY227.34c, MTCY9C4.01c), len: 884 aa. Conserved hypothetical ala-, leu-rich protein, equivalent to O53121|ML2679|MLCB1913.13 hypothetical protein from Mycobacterium leprae (893 aa), FASTA scores: opt: 4326,E(): 0, (75.2% identity in 883 aa overlap); and similar to Q49755|YO11_MYCLE|ML0605|MLCL536.05c|U1937B|B1937_F1_4 hypothetical 61.8 KDA protein from Mycobacterium leprae (561 aa), FASTA scores: opt: 758, E(): 1.2e-38, (32.2% identity in 537 aa overlap). Also similar to others e.g. Q9HUN7|PA4927 hypothetical protein from Pseudomonas aeruginosa (830 aa), FASTA scores: opt: 1247, E(): 2.2e-68,(38.25% identity in 831 aa overlap); Q98HG7|MLL2876 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (803 aa), FASTA scores: opt: 937, E(): 1.9e-49,(32.15% identity in 828 aa overlap); CAC47419|SMC04057 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (802 aa), FASTA scores: opt: 900,E(): 3.4e-47, (30.85% identity in 852 aa overlap); etc. And similar to P71732|YO11_MYCTU|Rv2411c|MT2484|MTCY253.09 conserved hypothetical protein from Mycobacterium tuberculosis (551 aa), FASTA scores: opt: 781, E(): 4.6e-40, (33.75% identity in 495 aa overlap).; Conserved hypothetical alanine and leucine rich protein 2892449 888578 Rv2567 Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine and leucine rich protein NP_217083.1 2889795 D 83332 CDS NP_217084.1 15609705 887249 complement(2892446..2893471) 1 NC_000962.3 Rv2568c, (MTCY227.33), len: 341 aa. Conserved hypothetical protein, highly similar (but longer 60 aa) to Q98E75|MLR4376 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (308 aa), FASTA scores: opt: 566, E(): 4.1e-29, (40.2% identity in 291 aa overlap).; hypothetical protein 2893471 887249 Rv2568c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217084.1 2892446 R 83332 CDS NP_217085.1 15609706 887783 complement(2893464..2894408) 1 NC_000962.3 Rv2569c, (MTCY227.32), len: 314 aa. Conserved hypothetical protein, equivalent to Q9CCT2|ML0508 hypothetical protein from Mycobacterium leprae (313 aa),FASTA scores: opt: 1723, E(): 1.9e-95, (84.4% identity in 301 aa overlap); and some similarity with Q49757|YP69_MYCLE|ML0607|MLCL536.03c|B1937_F2_39 hypothetical 31.1 KDA protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 305, E(): 4.5e-11, (33.0% identity in 300 aa overlap). Also similar to to other hypothetical proteins e.g. Q9HUN8|PA4926 from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 704, E(): 8.7e-35,(39.7% identity in 320 aa overlap); Q98HG8|MLL2875 from Rhizobium loti (Mesorhizobium loti) (294 aa), FASTA scores: opt: 521, E(): 6.5e-24, (35.05% identity in 294 aa overlap); Q9A7W9|CC1600 from Caulobacter crescentus (325 aa), FASTA scores: opt: 510, E(): 3.2e-23, (34.4% identity in 2588 aa overlap); etc. Also some similarity with proteins from Mycobacterium tuberculosis e.g. P71734|Rv2409c|MTCY253.11 conserved hypothetical protein (279 aa), FASTA scores: opt: 312, E(): 1.7e-11, (34.45% identity in 296 aa overlap); and Q50732|Rv2566|MTCY9C4.02 long conserved hypothetical protein (1140 aa), FASTA scores: opt: 252, E(): 2.2e-07, (28.9% identity in 315 aa overlap); hypothetical protein 2894408 887783 Rv2569c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217085.1 2893464 R 83332 CDS NP_217086.1 15609707 887377 2894512..2894901 1 NC_000962.3 Rv2570, (MTCY227.31c), len: 129 aa. Conserved hypothetical protein, similar to Q98GQ7|MLR3218 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (133 aa), FASTA scores: opt: 174, E(): 9.6e-05,(32.25% identity in 124 aa overlap); Q9A390|CC3314 hypothetical protein from Caulobacter crescentus (129 aa),FASTA scores: opt: 155, E(): 0.0017, (33.35% identity in 108 aa overlap); and Q9A2Y0|CC3426 hypothetical protein from Caulobacter crescentus (120 aa), FASTA scores: opt: 144, E(): 0.0083, (32.95% identity in 91 aa overlap).; hypothetical protein 2894901 887377 Rv2570 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217086.1 2894512 D 83332 CDS NP_217087.1 15609708 887382 complement(2894893..2895960) 1 NC_000962.3 Rv2571c, (MTCY227.30), len: 355 aa. Probable transmembrane ala-, val-, leu-rich protein, showing some similarity with other membrane proteins e.g. Q99340|YFDA_CORGL hypothetical integral membrane protein from Corynebacterium glutamicum (Brevibacterium flavum) (359 aa), FASTA scores: opt: 338, E(): 2.5e-13, (29.4% identity in 255 aa overlap); Q9RD86|SCF43.02 putative integral membrane protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 208, E(): 2.1e-05, (26.05% identity in 303 aa overlap); Q9RD81|SCF43.07 putative integral membrane protein from Streptomyces coelicolor (419 aa),FASTA scores: opt: 205, E(): 3.5e-05, (25.15% identity in 362 aa overlap); etc.; Probable transmembrane alanine and valine and leucine rich protein 2895960 887382 Rv2571c Mycobacterium tuberculosis H37Rv Probable transmembrane alanine and valine and leucine rich protein NP_217087.1 2894893 R 83332 CDS NP_217088.1 15609709 888532 complement(2896013..2897803) 1 NC_000962.3 Rv2572c, (MTCY227.29), len: 596 aa. Probable aspS,aspartyl-tRNA synthetase, equivalent to P36429|SYD_MYCLE|ML0501|MLCB1259.19 aspartyl-tRNA synthetase from Mycobacterium leprae (589 aa), FASTA scores: opt: 3534, E(): 1.8e-215, (87.85% identity in 592 aa overlap). Also highly similar to many e.g. O67589|SYD_AQUAE|AQ_1677 from Aquifex aeolicus (603 aa),FASTA scores: opt: 1829, E(): 8.2e-108, (47.5% identity in 598 aa overlap); O32038|SYD_BACSU from Bacillus subtilis (592 aa), FASTA scores: opt: 1732, E(): 1.1e-101, (46.25% identity in 597 aa overlap); P21889|SYD_ECOLI|TLS|B1866 from Escherichia coli strain K12 (590 aa), FASTA scores: opt: 1588, E(): 1.3e-92, (47.35% identity in 581 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family.; Probable aspartyl-tRNA synthetase AspS (aspartate--tRNA ligase) (ASPRS) (aspartic acid translase) 2897803 aspS 888532 aspS Mycobacterium tuberculosis H37Rv Probable aspartyl-tRNA synthetase AspS (aspartate--tRNA ligase) (ASPRS) (aspartic acid translase) NP_217088.1 2896013 R 83332 CDS NP_217089.3 448824788 888188 2898043..2898783 1 NC_000962.3 Rv2573, (MTCY227.28c), len: 246 aa. Conserved hypothetical protein, similar to various proteins e.g. Q9ABG6|CC0261 hypothetical protein from Caulobacter crescentus (290 aa), FASTA scores: opt: 516, E(): 5.8e-26,(40.1% identity in 237 aa overlap); Q99R37|SA2393 hypothetical protein (similar to 2-dehydropantoate 2-reductase) from Staphylococcus aureus subsp. aureus N315 (286 aa), FASTA scores: opt: 368, E(): 1.8e-16, (31.75% identity in 230 aa overlap); Q9KPQ9|VC2307 2-dehydropantoate 2-reductase from Vibrio cholerae (296 aa), FASTA scores: opt: 223, E(): 3.9e-07, (27.7% identity in 224 aa overlap); etc. Equivalent to AAK46962 from Mycobacterium tuberculosis strain CDC1551 (275 aa) but shorter 29 aa.; hypothetical protein 2898783 888188 Rv2573 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217089.3 2898043 D 83332 CDS NP_217090.1 15609711 887232 2898806..2899309 1 NC_000962.3 Rv2574, (MTCY227.27c), len: 167 aa. Conserved protein, showing similarity with Q9K3N3|SCG20A.07 hypothetical 17.4 KDA protein from Streptomyces coelicolor (157 aa), FASTA scores: opt: 218, E(): 2.8e-08, (30.65% identity in 150 aa overlap).; hypothetical protein 2899309 887232 Rv2574 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217090.1 2898806 D 83332 CDS NP_217091.1 15609712 888178 2899339..2900220 1 NC_000962.3 Rv2575, (MTCY227.26c), len: 293 aa. Possible conserved membrane gly-rich protein, highly similar to hypothetical proteins e.g. Q9RR98|DR2596 conserved hypothetical protein from Deinococcus radiodurans (313 aa),FASTA scores: opt: 734, E(): 2.8e-38, (42.95% identity in 291 aa overlap); Q9HV81|PA4717 from Pseudomonas aeruginosa (297 aa), FASTA scores: opt: 641, E(): 1.5e-32, (43.35% identity in 300 aa overlap); Q98IA4|MLL2493 from Rhizobium loti (Mesorhizobium loti) (306 aa), FASTA scores: opt: 628,E(): 1e-31, (38.45% identity in 307 aa overlap); etc. Contains PS00142 Neutral zinc metallopeptidases,zinc-binding region signature.; Possible conserved membrane glycine rich protein 2900220 888178 Rv2575 Mycobacterium tuberculosis H37Rv Possible conserved membrane glycine rich protein NP_217091.1 2899339 D 83332 CDS NP_217092.1 15609713 887251 complement(2900226..2900690) 1 NC_000962.3 Rv2576c, (MTCY227.25), len: 154 aa. Possible conserved membrane protein, showing similarity with Q9ZFC2 hypothetical 15.7 KDA protein from Mycobacterium sp. FM10 (146 aa), FASTA scores: opt: 235, E(): 4.1e-08, (31.35% identity in 150 aa overlap).; Possible conserved membrane protein 2900690 887251 Rv2576c Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_217092.1 2900226 R 83332 CDS NP_217093.1 15609714 888207 2900918..2902507 1 NC_000962.3 Rv2577, (MTCY227.24c), len: 529 aa. Conserved protein, showing similarity with various proteins from eukaryotes, in particular phosphatases, e.g. Q9SE01|pap purple acid phosphatase precursor from Glycine max (Soybean) (464 aa), FASTA scores: opt: 190, E(): 0.00026,(27.3% identity in 388 aa overlap); Q9SVP2|F18A5.90|AT4G13700 hypothetical 53.4 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (474 aa), FASTA scores: opt: 280, E(): 6.6e-10, (27.2% identity in 331 aa overlap); Q9FK32 similarity to unknown protein from Arabidopsis thaliana (Mouse-ear cress) (529 aa), FASTA scores: opt: 249, E(): 6.2e-08, (25.3% identity in 435 aa overlap); Q12546|APHA acid phosphatase precursor from Aspergillus ficuum (614 aa), FASTA scores: opt: 207, E(): 2.9e-05, (22.95% identity in 458 aa overlap); etc.; hypothetical protein 2902507 888207 Rv2577 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217093.1 2900918 D 83332 CDS NP_217094.1 15609715 887427 complement(2902509..2903531) 1 NC_000962.3 Rv2578c, (MTCY227.23), len: 340 aa. Conserved hypothetical protein, highly similar to hypothetical proteins (conserved or not) e.g. Q9ZBJ3|SC9C7.17c from Streptomyces coelicolor (348 aa), FASTA scores: opt: 998,E(): 1.6e-55, (47.6% identity in 355 aa overlap); Q9I763|PA0069 from Pseudomonas aeruginosa (352 aa), FASTA scores: opt: 560, E(): 6e-28, (36.6% identity in 284 aa overlap); Q986C9|MLL7417 from Rhizobium loti (Mesorhizobium loti) (356 aa), FASTA scores: opt: 550, E(): 2.6e-27,(39.15% identity in 240 aa overlap); etc.; hypothetical protein 2903531 887427 Rv2578c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217094.1 2902509 R 83332 CDS YP_177890.1 57117001 888599 2903639..2904541 1 NC_000962.3 Rv2579, (MTCY227.22c), len: 300 aa. Possible dhaA,haloalkane dehalogenase, strictly equivalent to Q9XB14|ISO-RV2579 haloalkane dehalogenase (1-chlorohexane halidohydrolase) from Mycobacterium bovis (300 aa), FASTA scores: opt: 2075, E(): 7.1e-125, (99.35% identity in 300 aa overlap); note that only two residues, 120 and 293 are different. Also highly similar to others e.g. Q9ZER0|DHAAF haloalkane dehalogenase from Mycobacterium sp strain GP1 (307 aa), FASTA scores: opt: 842, E(): 2.3e-46, (44.95% identity in 298 aa overlap); Q53042|DHAA haloalkane dehalogenase from Rhodococcus rhodochrous, and Pseudomonas pavonaceae (293 aa), FASTA scores: opt: 837, E(): 4.5e-46,(44.6% identity in 298 aa overlap); etc. Note that this protein may also be a 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase, because also highly similar to P51698|LINB_PSEPA 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (see Nagata et al., 1993) (296 aa), FASTA scores: opt: 1494,E(): 6.8e-88, (69.5% identity in 295 aa overlap). Also shows some similarity with proteins from Mycobacterium tuberculosis e.g. Q50670|YM96_MYCTU|Rv2296|MT2353|MTCY339.14c putative haloalkane dehalogenase (300 aa), FASTA scores: opt: 302,E(): 5.3e-12, (30.85% identity in 295 aa overlap); and Q50600|YJ33_MYCTU|Rv1833c|MT1881|MTCY1A11.10 hypothetical 32.2 KDA protein (286 aa), FASTA scores: opt: 286, E(): 5.3e-11, (29.85% identity in 288 aa overlap). May belong to alpha/beta hydrolase fold family. Note that previously known as linB.; Possible haloalkane dehalogenase DhaA (1-chlorohexane halidohydrolase) 2904541 dhaA 888599 dhaA Mycobacterium tuberculosis H37Rv Possible haloalkane dehalogenase DhaA (1-chlorohexane halidohydrolase) YP_177890.1 2903639 D 83332 CDS NP_217096.1 15609717 887479 complement(2904821..2906092) 1 NC_000962.3 Rv2580c, (MT2657, MTCY227.21), len: 423 aa. Probable hisS, histidyl-tRNA synthetase, equivalent to P46696|SYH_MYCLE|hiss|ML0494|MLCB1259.12|B1177_C3_248 histidyl-tRNA synthetase from Mycobacterium leprae (427 aa), FASTA scores: opt: 2380, E(): 2.1e-131, (85.85% identity in 417 aa overlap). Also highly similar to many e.g. Q9KXP2|hiss from Streptomyces coelicolor (425 aa),FASTA scores: opt: 1542, E(): 1.4e-82, (56.0% identity in 418 aa overlap); O32422|SYH_STAAU|hiss from Staphylococcus aureus (420 aa), FASTA scores: opt: 1135, E(): 7.4e-59,(44.9% identity in 412 aa overlap); P04804|SYH_ECOLI|hiss|B2514 from Escherichia coli strain K12 (423 aa), FASTA scores: opt: 1099, E(): 9.4e-57, (43.9% identity in 417 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to class-II aminoacyl-tRNA synthetase family.; Probable histidyl-tRNA synthetase HisS (histidine--tRNA ligase) (HISRS) (histidine--translase) 2906092 hisS 887479 hisS Mycobacterium tuberculosis H37Rv Probable histidyl-tRNA synthetase HisS (histidine--tRNA ligase) (HISRS) (histidine--translase) NP_217096.1 2904821 R 83332 CDS NP_217097.1 15609718 888217 complement(2906089..2906763) 1 NC_000962.3 Rv2581c, (MTCY227.20), len: 224 aa. Possible glyoxalase II, equivalent to Q49649|YP81_MYCLE|ML0493|MLCB1259.11|B1177_C3_247 hypothetical 23.9 KDA protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 1264, E(): 7.8e-73, (82.0% identity in 222 aa overlap). Also highly similar to Q9KXP1|SC9C5.33c possible hydrolase from Streptomyces coelicolor (235 aa), FASTA scores: opt: 654, E(): 2.9e-34,(46.8% identity in 220 aa overlap); and similar to Q9CI24|YFCI hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (210 aa), FASTA scores: opt: 360, E(): 9.9e-16, (35.0% identity in 217 aa overlap); AAK75726|SP1646 metallo-beta-lactamase superfamily protein from Streptococcus pneumoniae (209 aa),FASTA scores: opt: 320, E(): 3.3e-13, (35.85% identity in 198 aa overlap); AAK80229|CAC2272 predicted Zn-dependent hydrolase of metallo-beta-lactamase superfamily from Clostridium acetobutylicum (199 aa), FASTA scores: opt: 282, E(): 8e-11, (32.7% identity in 217 aa overlap); etc. Equivalent to AAK46971 from Mycobacterium tuberculosis strain CDC1551 (246 aa) but shorter 22 aa. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions.; Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) 2906763 888217 Rv2581c Mycobacterium tuberculosis H37Rv Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) NP_217097.1 2906089 R 83332 CDS NP_217098.1 15609719 887691 2906814..2907740 1 NC_000962.3 Rv2582, (MTCY227.19c), len: 308 aa. Probable ppiB (alternate gene name: ppi), cyclophilin (peptidyl-prolyl cis-trans isomerase), equivalent to P46697|PPIB_MYCLE|PPI|ML0492|MLCB1259.10c|B1177_F3_97 probable peptidyl-prolyl cis-trans isomerase B from Mycobacterium leprae (295 aa), FASTA scores: opt: 1423,E(): 1.3e-66, (72.2% identity in 295 aa overlap). Also similar to others e.g. Q9KJG8|PPIB peptidyl-prolyl cis-trans isomerase from Streptomyces lividans (277 aa),FASTA scores: opt: 485, E(): 3.2e-18, (38.35% identity in 292 aa overlap); Q9KXP0|SC9C5.34 peptidyl-prolyl cis-trans isomerase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 483, E(): 4.1e-18, (38.35% identity in 292 aa overlap); Q9RT72|DR1893 peptidyl-prolyl cis-trans isomerase from Deinococcus radiodurans (350 aa), FASTA scores: opt: 296, E(): 2.2e-08, (29.0% identity in 276 aa overlap); etc. Belongs to the cyclophilin-type PPIase family.; Probable peptidyl-prolyl cis-trans isomerase B PpiB (cyclophilin) (PPIase) (rotamase) (peptidylprolyl isomerase) 2907740 ppiB 887691 ppiB Mycobacterium tuberculosis H37Rv Probable peptidyl-prolyl cis-trans isomerase B PpiB (cyclophilin) (PPIase) (rotamase) (peptidylprolyl isomerase) NP_217098.1 2906814 D 83332 CDS NP_217099.1 15609720 887888 complement(2907826..2910198) 1 NC_000962.3 Rv2583c, (MTCY227.18), len: 790 aa. Probable relA,GTP pyrophosphokinase, equivalent to Q49640|RELA_MYCLE|ML0491|MLCB1259.09|B1177_C1_168 probable GTP pyrophosphokinase from Mycobacterium leprae (787 aa),FASTA scores: opt: 4834, E(): 0, (93.4% identity in 790 aa overlap). Also highly similar to others e.g. O87331|RELA_CORGL|RELA|rel from Corynebacterium glutamicum (Brevibacterium flavum) (760 aa), FASTA scores: opt: 3375,E(): 1.6e-196, (67.0% identity in 758 aa overlap); O85709|RELA_STRAT from Streptomyces antibioticus (841 aa),FASTA scores: opt: 3209, E(): 1.9e-186, (63.85% identity in 786 aa overlap); Q9KDH1|RELA|BH1242 from Bacillus halodurans (728 aa), FASTA scores: opt: 2195,E(): 3.8e-125,(45.65% identity in 714 aa overlap); etc. Belongs to the RELA / spot family.; Probable GTP pyrophosphokinase RelA (ATP:GTP 3'-pyrophosphotransferase) (PPGPP synthetase I) ((P)PPGPP synthetase) (GTP diphosphokinase) 2910198 relA 887888 relA Mycobacterium tuberculosis H37Rv Probable GTP pyrophosphokinase RelA (ATP:GTP 3'-pyrophosphotransferase) (PPGPP synthetase I) ((P)PPGPP synthetase) (GTP diphosphokinase) NP_217099.1 2907826 R 83332 CDS NP_217100.1 15609721 888579 complement(2910229..2910900) 1 NC_000962.3 Rv2584c, (MTCY227.17), len: 223 aa. Probable apt,adenine phosphoribosyltransferase, similar, but longer in N-terminus, to others e.g. O87330|APT_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (185 aa), FASTA scores: opt: 524, E(): 1.3e-24, (50.95% identity in 159 aa overlap); P52561|APT_STRCO from Streptomyces coelicolor (182 aa), FASTA scores: opt: 503, E(): 2.3e-23,(51.85% identity in 164 aa overlap); P47956|APT_MUSPA|APRT from Mus pahari (Shrew mouse) (180 aa), FASTA scores: opt: 419, E(): 2.5e-18, (44.7% identity in 170 aa overlap); P07672|P09993|P77121|APT_ECOLI|B0469 from Escherichia coli strain K12 (183 aa), FASTA scores: opt: 393, E(): 1.9e-18,(42.6% identity in 162 aa overlap); etc. Contains PS00103 Purine/ pyrimidine phosphoribosyl transferases signature,and PS00144 Asparaginase / glutaminase active site signature 1. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Nearest initiation codon indicated by homology is TTG at 17426 or GTG at 17465.; Adenine phosphoribosyltransferase Apt (APRT) (AMP diphosphorylase) (AMP pyrophosphorylase) (transphosphoribosidase) 2910900 apt 888579 apt Mycobacterium tuberculosis H37Rv Adenine phosphoribosyltransferase Apt (APRT) (AMP diphosphorylase) (AMP pyrophosphorylase) (transphosphoribosidase) NP_217100.1 2910229 R 83332 CDS NP_217101.1 15609722 887701 complement(2911004..2912677) 1 NC_000962.3 Rv2585c, (MT2662, MTCY227.16), len: 557 aa. Possible conserved lipoprotein precursor, possibly attached to the membrane by a lipid anchor and substrate-binding protein involved in transport, equivalent to Q49646|YP85_MYCLE|ML0489|MLCB1259.07|B1177_C2_197 hypothetical lipoprotein precursor from Mycobacterium leprae (555 aa), FASTA scores: opt: 2812, E(): 9.8e-158,(78.95% identity in 546 aa overlap); and C-terminus highly similar to C-terminus of Q49638|DCIAE|B1177_C1_166 DCIAE protein from Mycobacterium leprae (344 aa), FASTA scores: opt: 1177, E(): 7.4e-62, (78.6% identity in 229 aa overlap). Also similar in part to various proteins,principally substrate-binding proteins, e.g. O87329|DCIAE dipeptide-binding protein from Corynebacterium glutamicum (Brevibacterium flavum) (502 aa), FASTA scores: opt: 614,E(): 1.2e-28, (30.7% identity in 427 aa overlap); Q9AKR0|OPPA|CAC49261 putative oligopeptide uptake ABC transporter periplasmic solute-binding protein precursor from Rhizobium meliloti (Sinorhizobium meliloti) (532 aa),FASTA scores: opt: 209, E(): 7.7e-05, (22.85% identity in 460 aa overlap); P76128|YDDS_ECOLI|B1487|P77769|P76874 putative ABC transporter periplasmic binding protein from Escherichia coli strain K12 (516 aa), FASTA scores: opt: 182, E(): 0.0029, (20.0% identity in 315 aa overlap); etc.; Possible conserved lipoprotein 2912677 887701 Rv2585c Mycobacterium tuberculosis H37Rv Possible conserved lipoprotein NP_217101.1 2911004 R 83332 CDS NP_217102.1 15609723 887229 complement(2912683..2914011) 1 NC_000962.3 Rv2586c, (MT2663, MTCY227.15), len: 442 aa. Probable secF, protein-export membrane protein (integral membrane protein) (see citation below), equivalent to P38386|SECF_MYCLE|SECF|ML0488|MLCB1259.06|B1177_C3_239 protein-export membrane protein from Mycobacterium leprae (471 aa), FASTA scores: opt: 1910, E(): 2.9e-104, (72.15% identity in 456 aa overlap). Also similar to others e.g. Q9AE06|SECF from Corynebacterium glutamicum (Brevibacterium flavum) (403 aa), FASTA scores: opt: 1198, E(): 9.8e-63,(47.1% identity in 399 aa overlap); Q53956|SECF_STRCO|SCL2.05c from Streptomyces coelicolor (373 aa), FASTA scores: opt: 670, E(): 6.4e-32, (39.25% identity in 400 aa overlap); Q55611|SECF_SYNY3|SLR0775 from Synechocystis sp. strain PCC 6803 (315 aa), FASTA scores: opt: 416, E(): 3.8e-17, (33.8% identity in 296 aa overlap); etc. Belongs to the SECD/SECF family, SECF family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF,SECG|Rv1440 and SECY|Rv0732.; Probable protein-export membrane protein SecF 2914011 secF 887229 secF Mycobacterium tuberculosis H37Rv Probable protein-export membrane protein SecF NP_217102.1 2912683 R 83332 CDS NP_217103.1 15609724 888192 complement(2914015..2915736) 1 NC_000962.3 Rv2587c, (MTCY227.14), len: 573 aa. Probable secD,protein-export membrane protein (integral membrane protein) (see citation below), equivalent to P38387|SECD_MYCLE|ML0487|MLCB1259.05|B1177_C1_164 protein-export membrane protein from Mycobacterium leprae (571 aa), FASTA scores: opt: 2948, E(): 2.6e-97, (80.6% identity in 583 aa overlap). Also similar to others e.g. Q9AE07|SECD from Corynebacterium glutamicum (Brevibacterium flavum) (637 aa), FASTA scores: opt: 1023, E(): 1.9e-29,(44.95% identity in 596 aa overlap); Q53955|SECD_STRCO from Streptomyces coelicolor (570 aa), FASTA scores: opt: 864,E(): 7.2e-24, (38.0% identity in 584 aa overlap); O33517|SECD_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (554 aa), FASTA scores: opt: 551, E(): 7.6e-13, (32.25% identity in 304 aa overlap); etc. Equivalent to AAK46977 from Mycobacterium tuberculosis strain CDC1551 (554 aa) but longer 19 aa. Belongs to the SecD/SecF family, SecD family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732.; Probable protein-export membrane protein SecD 2915736 secD 888192 secD Mycobacterium tuberculosis H37Rv Probable protein-export membrane protein SecD NP_217103.1 2914015 R 83332 CDS NP_217104.1 15609725 887346 complement(2915846..2916193) 1 NC_000962.3 Rv2588c, (MTCY227.13), len: 115 aa. Probable yajC,secretion factor, a conserved membrane protein (see Braunstein & Belisle 2000), equivalent to Q49647|YP88_MYCLE|ML0486|MLCB1259.04|B1177_C3_235 hypothetical 12.8 KDA protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 499, E(): 2.7e-26, (77.0% identity in 100 aa overlap). Also similar to other proteins e.g. Q9AE08 hypothetical 13.5 KDA protein from Corynebacterium glutamicum (Brevibacterium flavum) (121 aa), FASTA scores: opt: 222, E(): 5e-08, (39.8% identity in 103 aa overlap); Q9L292|SCL2.07c putative secreted protein from Streptomyces coelicolor (169 aa), FASTA scores: opt: 203, E(): 1.2e-06, (32.05% identity in 106 aa overlap); Q9CDT0|YWAB unknown protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (110 aa), FASTA scores: opt: 150, E(): 0.0026, (30.85% identity in 94 aa overlap); etc.; Probable conserved membrane protein secretion factor YajC 2916193 yajC 887346 yajC Mycobacterium tuberculosis H37Rv Probable conserved membrane protein secretion factor YajC NP_217104.1 2915846 R 83332 CDS NP_217105.1 15609726 887915 2916360..2917709 1 NC_000962.3 Rv2589, (MTCY227.12c), len: 449 aa. Probable gabT,4-aminobutyrate aminotransferase, equivalent to P40829|GABT_MYCLE|ML0485|MLCB1259.03c|B1177_F2_67 4-aminobutyrate aminotransferase (446 aa), FASTA scores: opt: 2468, E(): 4.5e-141, (83.75% identity in 449 aa overlap). Also highly similar to others e.g. O86823|GABT from Streptomyces coelicolor (444 aa), FASTA scores: opt: 1832, E(): 8e-103, (63.9% identity in 443 aa overlap); AAK79395|CAC1427 from Clostridium acetobutylicum (445 aa),FASTA scores: opt: 1283, E(): 8.4e-70, (45.75% identity in 433 aa overlap); Q9KE66|BH0991 from Bacillus halodurans (443 aa), FASTA scores: opt: 1224, E(): 2.9e-66, (44.55% identity in 431 aa overlap); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate.; 4-aminobutyrate aminotransferase GabT (gamma-amino-N-butyrate transaminase) (GABA transaminase) (glutamate:succinic semialdehyde transaminase) (GABA aminotransferase) (GABA-at) 2917709 gabT 887915 gabT Mycobacterium tuberculosis H37Rv 4-aminobutyrate aminotransferase GabT (gamma-amino-N-butyrate transaminase) (GABA transaminase) (glutamate:succinic semialdehyde transaminase) (GABA aminotransferase) (GABA-at) NP_217105.1 2916360 D 83332 CDS NP_217106.1 15609727 888574 2917871..2921377 1 NC_000962.3 Rv2590, (MTCY227.11c), len: 1168 aa. Probable fadD9,fatty-acid-CoA synthetase, highly similar to O69484|FADD9 (alias Q9CCT4|FADD9|ML0484 but longer 14 aa) putative acyl-CoA synthetase from Mycobacterium leprae (1174 aa),FASTA scores: opt: 5247, E(): 0, (68.0% identity in 1178 aa overlap). N-terminal (approximately 700 residues) similar to other long chain fatty acid ligases. And C-terminus highly similar to C-terminus of Q9XCF2|PSTB PSTB protein from Mycobacterium avium (2552 aa), FASTA scores: opt: 2083, E(): 8.4e-116, (40.8% identity in 1150 aa overlap) (and weak similarity on N-terminus). C-terminal part highly similar to polyketide synthases and peptides synthases (weak similarity on N-terminus) e.g. Q10896|Rv0101|MTCY251.20|NRP probable peptide synthetase from Mycobacterium tuberculosis (2512 aa), FASTA scores: opt: 1988, E(): 3.7e-110, (40.2% identity in 1181 aa overlap); etc. Contains PS00455 putative AMP-binding domain signature, and PS00061 Short-chain alcohol dehydrogenase family signature. Seems to belong to the ATP-dependent AMP-binding enzyme family, and to the short-chain dehydrogenases/reductases (SDR) family.; Probable fatty-acid-CoA ligase FadD9 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 2921377 fadD9 888574 fadD9 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD9 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_217106.1 2917871 D 83332 CDS YP_177891.1 57117002 887992 2921551..2923182 1 NC_000962.3 Rv2591, (MTCY227.10c), len: 543 aa. PE_PGRS44,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. O53845|Rv0834c|MTV043.26c from Mycobacterium tuberculosis (882 aa), FASTA scores: opt: 1813, E(): 5.8e-66, (55.3% identity in 568 aa overlap). Equivalent to AAK46982 from Mycobacterium tuberculosis strain CDC1551 (505 aa) but longer 38 aa. Contains PS00583 pfkB family of carbohydrate kinases signature 1.; PE-PGRS family protein PE_PGRS44 2923182 PE_PGRS44 887992 PE_PGRS44 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS44 YP_177891.1 2921551 D 83332 CDS NP_217108.1 15609729 888173 complement(2923199..2924233) 1 NC_000962.3 Rv2592c, (MTCY227.09), len: 344 aa. Probable ruvB,Holliday junction binding protein (see Mizrahi & Andersen 1998), equivalent to P40833|RUVB_MYCLE|ML0483|B1177_C3_227 holliday junction DNA helicase from Mycobacterium leprae (349 aa), FASTA scores: opt: 2059, E(): 2.1e-106, (94.45% identity in 342 aa overlap). Also highly similar to others e.g. Q9AE09|RUVB from Corynebacterium glutamicum (Brevibacterium flavum) (363 aa), FASTA scores: opt: 1651,E(): 6.5e-84, (75.6% identity in 332 aa overlap); Q9L291|RUVB from Streptomyces coelicolor (357 aa), FASTA scores: opt: 1530, E(): 3e-77, (68.2% identity in 343 aa overlap); P08577|RUVB_ECOLI|B1860|Z2912|ECS2570 from Escherichia coli strains K12 and O157:H7 (336 aa), FASTA scores: opt: 1284, E(): 1e-63, (55.45% identity in 330 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the RuvB family.; Probable holliday junction DNA helicase RuvB 2924233 ruvB 888173 ruvB Mycobacterium tuberculosis H37Rv Probable holliday junction DNA helicase RuvB NP_217108.1 2923199 R 83332 CDS NP_217109.1 15609730 887688 complement(2924230..2924820) 1 NC_000962.3 Rv2593c, (MTCY227.08), len: 196 aa. Probable ruvA,Holliday junction binding protein (see citations below),equivalent to P40832|RUVA_MYCLE|ML0482|B1177_C2_188 holliday junction DNA helicase from Mycobacterium leprae (203 aa), FASTA scores: opt: 923, E(): 9.9e-50, (76.85% identity in 203 aa overlap). Also highly similar to others e.g. Q9L290|RUVA from Streptomyces coelicolor (201 aa) (201 aa), FASTA scores: opt: 549, E(): 8.2e-27, (47.55% identity in 204 aa overlap); Q9AE10|RUVA from Corynebacterium glutamicum (Brevibacterium flavum) (206 aa), FASTA scores: opt: 440, E(): 4e-20, (47.1% identity in 206 aa overlap); P08576|RUVA_ECOLI|B1861|Z2913|ECS2571 from Escherichia coli strains K12 and O157:H7 (203 aa), FASTA scores: opt: 312,E(): 2.8e-12, (34.85% identity in 201 aa overlap); etc. Belongs to the RuvA family.; Probable holliday junction DNA helicase RuvA 2924820 ruvA 887688 ruvA Mycobacterium tuberculosis H37Rv Probable holliday junction DNA helicase RuvA NP_217109.1 2924230 R 83332 CDS NP_217110.1 15609731 887418 complement(2924817..2925383) 1 NC_000962.3 Rv2594c, (MTCY227.07), len: 188 aa. Probable ruvC,Holliday junction resolvase (see citations below),equivalent to P40834|RUVC_MYCLE|ML0481|B1177_C3_226 crossover junction endodeoxyribonuclease from Mycobacterium leprae (188 aa), FASTA scores: opt: 984, E(): 2.3e-55,(81.0% identity in 184 aa overlap). Also highly similar to others e.g. Q9AE11|RUVC from Corynebacterium glutamicum (Brevibacterium flavum) (221 aa), FASTA scores: opt: 713,E(): 3.6e-38, (56.9% identity in 188 aa overlap); Q9L289|RUVC_STRCO|SCL2.10c from Streptomyces coelicolor (188 aa), FASTA scores: opt: 704, E(): 1.2e-37, (60.65% identity in 178 aa overlap); P24239|RUVC_ECOLI|B1863 from Escherichia coli strain K12 (172 aa), FASTA scores: opt: 322, E(): 1.6e-13, (38.65% identity in 163 aa overlap); etc. Belongs to the RUVC family. Cofactor: magnesium.; Probable crossover junction endodeoxyribonuclease RuvC (holliday junction nuclease) (holliday junction resolvase) 2925383 ruvC 887418 ruvC Mycobacterium tuberculosis H37Rv Probable crossover junction endodeoxyribonuclease RuvC (holliday junction nuclease) (holliday junction resolvase) NP_217110.1 2924817 R 83332 CDS NP_217111.1 15609732 887682 2925492..2925737 1 NC_000962.3 Rv2595, (MTCY227.06c), len: 81 aa. Possible vapB40,antitoxin, part of toxin-antitoxin (TA) operon with Rv2596,see Arcus et al. 2005. Similarity with various bacterial proteins e.g. O28268|AF2011 conserved hypothetical protein from Archaeoglobus fulgidus (86 aa), FASTA scores: opt: 120, E(): 0.13, (34.35% identity in 67 aa overlap); CAC46196|SMC01176 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (79 aa), FASTA scores: opt: 119, E(): 0.14, (33.35% identity in 63 aa overlap); P37554|SP5T_BACSU|SPOVT stage V sporulation protein T from Bacillus subtilis (178 aa), FASTA scores: opt: 104, E(): 2.9, (51.45% identity in 35 aa overlap); etc. Also similar to O07779|Rv0599c|MTCY19H5.23 hypothetical protein from Mycobacterium tuberculosis (78 aa), FASTA scores: opt: 160, E(): 0.00026, (35.8% identity in 81 aa overlap).; Possible antitoxin VapB40 2925737 vapB40 887682 vapB40 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB40 NP_217111.1 2925492 D 83332 CDS NP_217112.1 15609733 888218 2925734..2926138 1 NC_000962.3 Rv2596, (MTCY227.05c), len: 134 aa. Possible vapC40,toxin, part of toxin-antitoxin (TA) operon with Rv2595,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O07780|Rv0598c|MTCY19H5.24 hypothetical 14.8 KDA protein from (137 aa), FASTA scores: opt: 254, E(): 8.8e-11,(41.55% identity in 130 aa overlap).; Possible toxin VapC40 Contains PIN domain 2926138 vapC40 888218 vapC40 Mycobacterium tuberculosis H37Rv Possible toxin VapC40 Contains PIN domain NP_217112.1 2925734 D 83332 CDS NP_217113.1 15609734 887679 2926355..2926975 1 NC_000962.3 Rv2597, (MTCY227.04c), len: 206 aa. Probable membrane protein. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable membrane protein 2926975 887679 Rv2597 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_217113.1 2926355 D 83332 CDS NP_217114.1 15609735 887689 2926986..2927480 1 NC_000962.3 Rv2598, (MTCY227.03c), len: 164 aa. Conserved hypothetical protein, showing similarity with hypothetical proteins from Streptomyces coelicolor e.g. Q9X8S3|SCH10.34c (185 aa), FASTA scores: opt: 197, E(): 3.5e-06, (34.75% identity in 167 aa overlap); and Q9L088|SCC24.29c (172 aa),FASTA scores: opt: 149, E(): 0.0053, (37.65% identity in 146 aa overlap). Equivalent to AAK46988 from Mycobacterium tuberculosis strain CDC1551 (154 aa) but longer 10 aa.; hypothetical protein 2927480 887689 Rv2598 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217114.1 2926986 D 83332 CDS NP_217115.1 15609736 887832 2927477..2927908 1 NC_000962.3 Rv2599, (MTCY227.02c), len: 143 aa. Probable conserved membrane protein, equivalent to Q9K536|2599 hypothetical 15.0 KDA protein (fragment) from Mycobacterium paratuberculosis (143 aa), FASTA scores: opt: 691, E(): 1.7e-33, (68.55% identity in 143 aa overlap). Shows weak similarity with Q9L089|SCC24.28c putative lipoprotein from Streptomyces coelicolor (131 aa), FASTA scores: opt: 130,E(): 0.52, (26.45% identity in 136 aa overlap). Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein 2927908 887832 Rv2599 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_217115.1 2927477 D 83332 CDS NP_217116.1 15609737 887869 2927990..2928391 1 NC_000962.3 Rv2600, (MTCY277.01c, MTV001.01), len: 133 aa. Probable conserved integral membrane protein, equivalent (but shorter 18 aa) to Q9K537|YQ00_MYCPA hypothetical protein RV2600 homolog from Mycobacterium paratuberculosis (151 aa), FASTA scores: opt: 543, E(): 4.2e-28, (62.9% identity in 132 aa overlap). Also some similarity with other hypothetical or membrane proteins e.g. Q9L090|SCC24.27c putative integral membrane protein from Streptomyces coelicolor (146 aa), FASTA scores: opt: 241,E(): 8.7e-09, (34.8% identity in 135 aa overlap); O58487|PH0773 hypothetical 15.0 KDA protein from Pyrococcus horikoshii (138 aa), FASTA scores: opt: 116, E(): 0.84,(34.35% identity in 96 aa overlap); etc. Equivalent to AAK46990 from Mycobacterium tuberculosis strain CDC1551 (152 aa) but shorter 19 aa.; Probable conserved integral membrane protein 2928391 887869 Rv2600 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217116.1 2927990 D 83332 CDS YP_177892.1 57117003 887676 2928388..2929959 1 NC_000962.3 Rv2601, (MTCI270.04c-MTV001.02), len: 523 aa. Probable speE, spermidine synthase, highly similar to many e.g. Q9L091|SCC24.26c from Streptomyces coelicolor (531 aa), FASTA scores: opt: 1493, E(): 1.3e-79, (48.45% identity in 514 aa overlap); Q9X8S2|SCH10.33c from Streptomyces coelicolor (554 aa), FASTA scores: opt: 1045,E(): 1.7e-53, (40.55% identity in 525 aa overlap); P09158|SPEE_ECOLI|B0121 from Escherichia coli strain K12 (287 aa), FASTA scores: opt: 368, E(): 2.9e-14, (30.5% identity in 272 aa overlap); etc.; Probable spermidine synthase SpeE (putrescine aminopropyltransferase) (aminopropyltransferase) (SPDSY) 2929959 speE 887676 speE Mycobacterium tuberculosis H37Rv Probable spermidine synthase SpeE (putrescine aminopropyltransferase) (aminopropyltransferase) (SPDSY) YP_177892.1 2928388 D 83332 CDS YP_177673.1 57117004 3205108 2930070..2930357 1 NC_000962.3 Rv2601A, len: 95 aa. Possible vapB41, antitoxin,part of toxin-antitoxin (TA) operon with Rv2602, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O53811|Rv0748 conserved hypothetical protein (88 aa), FASTA scores: opt: 132, E(): 0.017,(29.25% identity in 82 aa overlap); O53218|Rv2493 (73 aa),FASTA scores: opt: 107, E(): 0.97, (33.75% identity in 83 aa overlap); and Q10799|YS71_MYCTU|Rv2871 conserved hypothetical protein from Mycobacterium tuberculosis (85 aa), FASTA scores: opt: 108, E(): 0.91, (41.00% identity in 39 aa overlap).; Possible antitoxin VapB41 2930357 vapB41 3205108 vapB41 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB41 YP_177673.1 2930070 D 83332 CDS NP_217118.1 15609739 888186 2930344..2930784 1 NC_000962.3 Rv2602, (MTCI270A.03c), len: 146 aa. Possible vapC41, toxin, part of toxin-antitoxin (TA) operon with Rv2601A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O50457|Rv1242|MTV006.14 (143 aa),FASTA scores: opt: 147, E(): 0.0021, (26.25% identity in 141 aa overlap); P95023|Rv2530c|MTCY159.26 (139 aa), FASTA scores: opt: 131, E(): 0.027, (33.35% identity in 135 aa overlap); O53812|Rv0749|MTV041.23 (142 aa), FASTA scores: opt: 125, E(): 0.072, (26.45% identity in 140 aa overlap).; Possible toxin VapC41 Contains PIN domain 2930784 vapC41 888186 vapC41 Mycobacterium tuberculosis H37Rv Possible toxin VapC41 Contains PIN domain NP_217118.1 2930344 D 83332 CDS NP_217119.1 15609740 887369 complement(2930805..2931560) 1 NC_000962.3 Rv2603c, (MTCI270A.02), len: 251 aa. Highly conserved protein, equivalent to Q49645|YQ03_MYCLE|ML0475|U1177B|B1177_C2_181 hypothetical 26.6 KDA protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1514, E(): 2.2e-84, (92.45% identity in 251 aa overlap). Also highly similar to Q9L288|SCL2.11c hypothetical 26.8 KDA protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 1268, E(): 1.5e-69, (76.7% identity in 249 aa overlap); Q9AE12|YFCA hypothetical structural protein from Corynebacterium glutamicum (Brevibacterium flavum) (251 aa), FASTA scores: opt: 1231,E(): 2.6e-67, (72.9% identity in 251 aa overlap); O83487|Y474_TREPA|TP0474 hypothetical protein from Treponema pallidum (245 aa), FASTA scores: opt: 780, E(): 4.4e-40, (47.75% identity in 245 aa overlap); P24237|YEBC_ECOLI|B1864 protein YEBC from Escherichia coli strain K12 (246 aa), FASTA scores: opt: 776, E(): 7.6e-40,(47.8% identity in 249 aa overlap); etc.; Highly conserved protein 2931560 887369 Rv2603c Mycobacterium tuberculosis H37Rv Highly conserved protein NP_217119.1 2930805 R 83332 CDS NP_217120.1 15609741 887371 complement(2931693..2932289) 1 NC_000962.3 Rv2604c, (MTCY01A10.29, MTCI270A.01), len: 198 aa. Probable snoP, glutamine amidotransferase, equivalent (but shorter 21 aa) to Q49637|HISH|B1177_C1_149 HISH protein (belongs to the YFL060C/YAAE/HI1648 family) (alias Q9CCT5|ML0474 hypothetical protein 223 aa) from Mycobacterium leprae (219 aa), FASTA scores: opt: 1069,E(): 1.7e-60, (83.35% identity in 198 aa overlap). Also highly similar to hypothetical proteins or amidotransferases e.g. Q9L287|SCL2.12c hypothetical 21.5 KDA protein from Streptomyces coelicolor (202 aa), FASTA scores: opt: 702, E(): 2.3e-37, (56.75% identity in 192 aa overlap); P37528|YAAE_BACSU hypothetical 21.4 KDA protein from Bacillus subtilis (196 aa), FASTA scores: opt: 608,E(): 1.9e-31, (48.7% identity in 189 aa overlap); Q9KGN5|BH0023 amidotransferase from Bacillus halodurans (196 aa), FASTA scores: opt: 583, E(): 7.4e-30, (48.7% identity in 195 aa overlap); etc. Also some similarity with several proteins from Mycobacterium tuberculosis e.g. O06589|HIS5_MYCTU|Rv1602|MT1638|MTCY336.02c amidotransferase (206 aa), FASTA scores: opt: 154, E(): 0.00036, (30.6% identity in 193 aa overlap). Contains a Pfam match to entry PF01174 SNO glutamine amidotransferase family. Note possibly co-regulated with snzP (Rv2606c).; Probable glutamine amidotransferase SnoP 2932289 snoP 887371 snoP Mycobacterium tuberculosis H37Rv Probable glutamine amidotransferase SnoP NP_217120.1 2931693 R 83332 CDS NP_217121.1 15609742 887588 complement(2932297..2933142) 1 NC_000962.3 Rv2605c, (MTCY01A10.28), len: 281 aa. Probable tesB2, acyl-CoA thioesterase II, highly similar to others e.g. Q98EG9|MLL4250 from Rhizobium loti (Mesorhizobium loti) (286 aa), FASTA scores: opt: 563, E(): 3.9e-29,(47.75% identity in 287 aa overlap); CAC47767 from Rhizobium meliloti (Sinorhizobium meliloti) (294 aa), FASTA scores: opt: 553, E(): 1.8e-28, (49.3% identity in 280 aa overlap); P23911|TESB_ECOLI|B0452 from Escherichia coli strain K12 (285 aa), FASTA scores: opt: 487, E(): 3.1e-24,(41.9% identity in 277 aa overlap); etc. Also similar to O06135|TESB1|Rv1618|MTCY01B2.10 acyl-CoA thioesterase II from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 425, E(): 1.1e-21, (34.9% identity in 278 aa overlap). Belongs to the C/M/P thioester hydrolase family.; Probable acyl-CoA thioesterase II TesB2 (TEII) 2933142 tesB2 887588 tesB2 Mycobacterium tuberculosis H37Rv Probable acyl-CoA thioesterase II TesB2 (TEII) NP_217121.1 2932297 R 83332 CDS NP_217122.1 15609743 888592 complement(2933171..2934070) 1 NC_000962.3 Rv2606c, (MTCY01A10.27), len: 299 aa. Probable snzP,pyridoxine biosynthesis protein. Highly similar to O07145|YQ06_MYCLE|ML0450|MLCL581.12c possible pyridoxine biosynthesis protein from Mycobacterium leprae (307 aa),FASTA scores: opt: 1686, E(): 1.5e-95, (89.7% identity in 291 aa overlap). Also highly similar to several pyridoxine biosynthesis proteins and hypothetical proteins e.g. Q9L286|SCL2.13c hypothetical 32.2 KDA protein from Streptomyces coelicolor (303 aa), FASTA scores: opt: 1461,E(): 7.6e-82, (76.8% identity in 293 aa overlap); O14027|YEM4_SCHPO|SPAC29B12.04 putative stress-induced protein from Schizosaccharomyces pombe (Fission yeast) (296 aa), FASTA scores: opt: 1318, E(): 3.8e-73, (70.35% identity in 290 aa overlap); Q9UW83|PYROA protein involved in pyridoxine biosynthesis from Emericella nidulans (Aspergillus nidulans) (see citation below) (304 aa), FASTA scores: opt: 1288, E(): 2.6e-71, (67.9% identity in 302 aa overlap); etc. Contains Pfam match to entry PF01680,SOR_SNZ family. Contains PS01235 Uncharacterized protein family UPF0019 signature. Belongs to the SOR_SNZ family. Note possibly co-regulated with snoP (Rv2604c).; Possible pyridoxine biosynthesis protein SnzP 2934070 snzP 888592 snzP Mycobacterium tuberculosis H37Rv Possible pyridoxine biosynthesis protein SnzP NP_217122.1 2933171 R 83332 CDS NP_217123.1 15609744 888155 2934198..2934872 1 NC_000962.3 Rv2607, (MTCY01A10.26c), len: 224 aa. Probable pdxH,pyridoxinephosphate oxidase, equivalent to O33065|PDXH_MYCLE|ML2131|MLCB57.46 pyridoxamine 5'-phosphate oxidase from Mycobacterium leprae (219 aa),FASTA scores: opt: 1038, E(): 8.3e-61, (67.1% identity in 219 aa overlap). Also similar to others e.g. Q9I4S5|PDXH|PA1049 from Pseudomonas aeruginosa (215 aa),FASTA scores: opt: 608, E(): 1.1e-32, (49.55% identity in 218 aa overlap); Q9K3V7|SCD10.19c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 600, E(): 3.9e-32,(42.3% identity in 234 aa overlap); P28225|PDXH_ECOLI|B1638 from Escherichia coli strain K12 (217 aa), FASTA scores: opt: 533, E(): 8.9e-28, (40.3% identity in 216 aa overlap); etc. Contains a match to Pfam entry PF01243 Pyridoxamine 5'-phosphate oxidase. Belongs to the pyridoxamine 5'-phosphate oxidase family. Cofactor: FMN.; Probable pyridoxamine 5'-phosphate oxidase PdxH (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) 2934872 pdxH 888155 pdxH Mycobacterium tuberculosis H37Rv Probable pyridoxamine 5'-phosphate oxidase PdxH (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) NP_217123.1 2934198 D 83332 CDS YP_177893.1 57117005 888204 2935046..2936788 1 NC_000962.3 Rv2608, (MTCY01A10.25c), len: 580 aa. PPE42, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O06828|Rv1430|MTCY493.24c from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1004, E(): 5.9e-48, (56.05% identity in 307 aa overlap).; PPE family protein PPE42 2936788 PPE42 888204 PPE42 Mycobacterium tuberculosis H37Rv PPE family protein PPE42 YP_177893.1 2935046 D 83332 CDS NP_217125.1 15609746 888208 complement(2936810..2937865) 1 NC_000962.3 Rv2609c, (MTCY01A10.24), len: 351 aa. Probable conserved membrane protein, equivalent to O07146|MLCL581.13c|ML0451 hypothetical 37.9 KDA protein from Mycobacterium leprae (349 aa), FASTA scores: opt: 1675, E(): 1.4e-95, (77.85% identity in 334 aa overlap). Also similar to hypothetical proteins: O69888|SC2E1.17|mutt hypothetical 19.4 KDA protein from Streptomyces coelicolor and Streptomyces lividans (172 aa), FASTA scores: opt: 345,E(): 3.5e-14, (44.7% identity in 161 aa overlap); Q9L285|SCL2.14c hypothetical 19.8 KDA protein from Streptomyces coelicolor (180 aa), FASTA scores: opt: 179,E(): 0.00056, (43.25% identity in 171 aa overlap); and Q9RYE5|DR0004 mutt/NUDIX family protein from Deinococcus radiodurans (350 aa), FASTA scores: opt: 153, E(): 0.037,(33.35% identity in 123 aa overlap). Contains PS00893 mutT domain signature. Belongs to the mutt/NUDIX family.; Probable conserved membrane protein 2937865 888208 Rv2609c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_217125.1 2936810 R 83332 CDS NP_217126.1 15609747 888627 complement(2937865..2939001) 1 NC_000962.3 Rv2610c, (MTCY01A10.23), len: 378 aa. PimA,alpha-mannosyltransferase (see citations below), equivalent to O07147|MLCL581.14c|ML0452 putative glycosyltransferase from Mycobacterium leprae (374 aa), FASTA scores: opt: 2044, E(): 8.8e-118, (82.25% identity in 378 aa overlap). N-terminus (from aa 1 to 27) equivalent to Q9FY7 putative alpha-mannosyl transferase (fragment) from Mycobacterium smegmatis (27 aa), blastp scores: 57.4 bits (137), E(): 3e-8, Identities = 25/27 (92%), Positives = 27/27 (99%) (see citation below). Also highly similar to Q9L284|SCL2.15c putative sugar transferase from Streptomyces coelicolor (387 aa), FASTA scores: opt: 1222,E(): 1.8e-67, (52.95% identity in 376 aa overlap); and similar in part to various proteins e.g. Q9YA73|APE2066 long hypothetical N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein from Aeropyrum pernix (392 aa), FASTA scores: opt: 434, E(): 3e-19, (31.5% identity in 378 aa overlap); Q9UZA1|PAB0827 galactosyltransferase or LPS biosynthesis RFBU related protein from Pyrococcus abyssi (371 aa), FASTA scores: opt: 382, E(): 4.3e-16, (28.2% identity in 383 aa overlap); O26275|MTH173 LPS biosynthesis RFBU related protein from Methanothermobacter thermautotrophicus (382 aa), FASTA scores: opt: 372, E(): 1.8e-15, (28.4% identity in 391 aa overlap); etc. Shows also some similarity with O05313|Rv1212c|MTCI364.24c hypothetical 41.5 KDA protein from Mycobacterium tuberculosis (387 aa), FASTA scores: opt: 232, E(): 1.1e -07, (28.4% identity in 402 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Alpha-mannosyltransferase PimA 2939001 pimA 888627 pimA Mycobacterium tuberculosis H37Rv Alpha-mannosyltransferase PimA NP_217126.1 2937865 R 83332 CDS NP_217127.1 15609748 888618 complement(2939012..2939962) 1 NC_000962.3 Rv2611c, (MTCY01A10.22), len: 316 aa. Probable acyltransferase , equivalent to O07148|MLCL581.15c|ML0453 hypothetical 35.4 KDA protein from Mycobacterium leprae (320 aa), FASTA scores: opt: 1529, E(): 5e-90, (71.45% identity in 312 aa overlap); and equivalent to Q9F7Y8 putative acyltransferase from Mycobacterium smegmatis (303 aa), FASTA scores: opt: 1464, E(): 6.5e-86, (72.15% identity in 291 aa overlap) (see citation below). Also highly similar to Q9L283|SCL2.16c putative acyltransferase from Streptomyces coelicolor (311 aa), FASTA scores: opt: 810, E(): 2.8e-44, (47.7% identity in 302 aa overlap); and similar to other acyltransferases e.g. Q9F0N3 acyltransferase from Campylobacter jejuni (295 aa), FASTA scores: opt: 207, E(): 6.4e-06, (20.45% identity in 220 aa overlap); Q9K379 acyltransferase (lipid a biosynthesis acyltransferase) from Campylobacter jejuni (295 aa), FASTA scores: opt: 203, E(): 1.1e-05, (20.0% identity in 220 aa overlap); etc.; Probable acyltransferase 2939962 888618 Rv2611c Mycobacterium tuberculosis H37Rv Probable acyltransferase NP_217127.1 2939012 R 83332 CDS YP_177894.1 57117006 888209 complement(2939959..2940612) 1 NC_000962.3 Rv2612c, (MTCY01A10.21), len: 217 aa. pgsA1 (previously known as pgsA), PI synthase/CDP-diacylglyceride--inositol phosphatidyltransferase, transmembrane protein, equivalent to O07149|MLCL581.16c|PGSA|ML0454 putative phosphatidyltransferase from Mycobacterium leprae (239 aa),FASTA scores: opt: 1141, E(): 4.1e-70, (79.35% identity in 213 aa overlap); and Q9F7Y9|PGSA phosphatidylinositol synthase from Mycobacterium smegmatis (222 aa), FASTA scores: opt: 981, E(): 2.7e-59, (67.3% identity in 217 aa overlap) (see citation below). Also similar to other proteins e.g. Q9L282|SCL2.17c putative membrane transferase from Streptomyces coelicolor (241 aa), FASTA scores: opt: 564, E(): 4.9e-31, (43.4% identity in 212 aa overlap); Q9UYD0|PGSA-like|PAB1041 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Pyrococcus abyssi (186 aa),FASTA scores: opt: 264, E(): 8.4e-11, (33.15% identity in 190 aa overlap); Q9HQS2|PGSA|VNG1030G CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase from Halobacterium sp. strain NRC-1 (199 aa), FASTA scores: opt: 249, E(): 9.1e-10,(32.1% identity in 193 aa overlap); etc. Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. Note that in Mycobacterium smegmatis, the psgA homologue is essential to the survival of the bacteria and seems cannot be compensated by any other enzyme of Mycobacterium smegmatis.; PI synthase PgsA1 (phosphatidylinositol synthase) (CDP-diacylglycerol--inositol-3-phosphatidyltransferase) 2940612 pgsA1 888209 pgsA1 Mycobacterium tuberculosis H37Rv PI synthase PgsA1 (phosphatidylinositol synthase) (CDP-diacylglycerol--inositol-3-phosphatidyltransferase) YP_177894.1 2939959 R 83332 CDS NP_217129.1 15609750 888193 complement(2940609..2941196) 1 NC_000962.3 Rv2613c, (MTCY01A10.20A), len: 195 aa. Conserved protein, equivalent to Q9CCU0|ML0455 hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 1074, E(): 7.4e-62, (84.7% identity in 196 aa overlap); and highly similar, but longer 18 aa, to O07150|MLCL581.17c hypothetical 20.7 KDA protein from Mycobacterium leprae (186 aa), FASTA scores: opt: 1038, E(): 1.4e-59, (89.7% identity in 175 aa overlap). Also highly similar to other hypothetical proteins (often Hit family member) e.g. Q9F7Z0 from Mycobacterium smegmatis (see citation below) (205 aa),FASTA scores: opt: 975, E(): 1.6e-55, (79.35% identity in 184 aa overlap); Q9L279|SCL2.20 from Streptomyces coelicolor (186 aa), FASTA scores: opt: 638, E(): 5.8e-34,(52.85% identity in 176 aa overlap); Q9YFX8|APE0122 from Aeropyrum pernix (184 aa), FASTA scores: opt: 515, E(): 4.4e-26, (45.9% identity in 159 aa overlap); etc. It seems the Rv2613c and downstream ORF Rv2612c|psgA1 are expressed from the same promoter (see citation below) and that Rv2613c should be involved in lipid metabolism.; hypothetical protein 2941196 888193 Rv2613c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217129.1 2940609 R 83332 CDS NP_217130.1 15609751 888211 complement(2941189..2943267) 1 NC_000962.3 Rv2614c, (MT2689, MTCY01A10.20), len: 692 aa. Probable thrS, threonyl-tRNA synthetase (Threonine--tRNA ligase), equivalent to O07151|SYT_MYCLE|THRS|ML0456|MLCL581.18c threonyl-tRNA synthetase from Mycobacterium leprae (702 aa), FASTA scores: opt: 3988, E(): 0, (84.05% identity in 702 aa overlap). Also highly similar to others e.g. Q9L278|THRS from Streptomyces coelicolor (658 aa), FASTA scores: opt: 1982, E(): 5.1e-114, (65.1% identity in 659 aa overlap); P56881|SYT_THETH|THRS from Thermus aquaticus (subsp. thermophilus) (659 aa), FASTA scores: opt: 1551, E(): 1.5e-87, (46.5% identity in 650 aa overlap); P00955|SYT_ECOLI from Escherichia coli (642 aa), FASTA scores: opt: 946, E(): 0, (40.7% identity in 612 aa overl ap); etc. Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. Belongs to class-II aminoacyl-tRNA synthetase family. Cofactor: binds 1 zinc ion (by similarity).; Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase) 2943267 thrS 888211 thrS Mycobacterium tuberculosis H37Rv Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase) NP_217130.1 2941189 R 83332 CDS YP_177674.1 57117007 3205055 2943376..2943603 1 NC_000962.3 Rv2614A, len: 75 aa. Conserved hypothetical protein. The region from aa 10-35 is similar to part of C-terminal part of several triosephosphate isomerases e.g. P46711|TPIS_MYCLE|TPIA|TPI|ML0572|B1496_C1_127 from Mycobacterium leprae (261 aa), FASTA scores: opt: 112, E(): 0.95, (60.0% identity in 25 aa overlap); and O08408|TPIS_MYCTU|TPIA|TPI|Rv1438|MT1482|MTCY493.16c from Mycobacterium tuberculosis (261 aa), FASTA scores: opt: 104, E(): 3.3, (60.0% identity in 25 aa overlap); P19583|TPIS_CORGL|TPIA|TPI from Corynebacterium glutamicum (Brevibacterium flavum) (259 aa), FASTA scores: opt: 100,E(): 6, (45.45% identity in 33 aa overlap); etc. Triosephosphate isomerases play an important role in several metabolic pathways (catalytic activity: D-glyceraldehyde 3-phosphate = dihydroxy-acetone phosphate). Nucleotide position 2943411 in the genome sequence has been corrected, T:C resulting in L12L.; hypothetical protein 2943603 3205055 Rv2614A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177674.1 2943376 D 83332 CDS YP_177895.1 57117008 888215 complement(2943600..2944985) 1 NC_000962.3 Rv2615c, (MTCY01A10.19), len: 461 aa. PE_PGRS45,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many e.g. P71664|Rv1396c|MTCY21B4.13c from Mycobacterium tuberculosis (576 aa), FASTA scores: opt: 1629, E(): 4.8e-58, (56.65% identity in 482 aa overlap). Equivalent to AAK47006 from Mycobacterium tuberculosis strain CDC1551 (476 aa) but shorter 15 aa.; PE-PGRS family protein PE_PGRS45 2944985 PE_PGRS45 888215 PE_PGRS45 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS45 YP_177895.1 2943600 R 83332 CDS NP_217132.1 15609753 887289 2945330..2945830 1 NC_000962.3 Rv2616, (MTCY01A10.18c), len: 166 aa. Conserved protein, highly similar to bacterial proteins: Q9L1G0|SC3D11.02c hypothetical 20.3 KDA protein from Streptomyces coelicolor (188 aa), FASTA scores: opt: 407,E(): 2.3e-20, (44.0% identity in 159 aa overlap); Q9X945 A3(2) glycogen metabolism cluster from Streptomyces coelicolor (134 aa), FASTA scores: opt: 330, E(): 2.5e-15,(46.65% identity in 120 aa overlap) (N-terminus shorter); Q9RST8|DR2035 conserved hypothetical protein from Deinococcus radiodurans (198 aa), FASTA scores: opt: 228,E(): 2.4e-08, (35.1% identity in 168 aa overlap).; hypothetical protein 2945830 887289 Rv2616 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217132.1 2945330 D 83332 CDS NP_217133.1 15609754 888610 complement(2945847..2946287) 1 NC_000962.3 Rv2617c, (MTCY01A10.17), len: 146 aa. Probable transmembrane protein, showing some similarity to hypothetical or membrane proteins e.g. CAC47207|SMC00744 putative transport protein transmembrane from Rhizobium meliloti (Sinorhizobium meliloti) (399 aa), FASTA scores: opt: 108, E(): 5.5, (29.15% identity in 144 aa overlap).; Probable transmembrane protein 2946287 888610 Rv2617c Mycobacterium tuberculosis H37Rv Probable transmembrane protein NP_217133.1 2945847 R 83332 CDS NP_217134.1 15609755 887690 2946434..2947111 1 NC_000962.3 Rv2618, (MTCY01A10.15c), len: 225 aa. Conserved hypothetical protein, similar in part to Q9EWQ9|SC4C2.03 conserved hypothetical protein from Streptomyces coelicolor (159 aa), FASTA scores: opt: 235, E(): 1.3e-07, (43.7% identity in 103 aa overlap); Q9HLM6|TA0201 hypothetical protein from Thermoplasma acidophilum (215 aa), FASTA scores: opt: 164, E(): 0.0038, (23.4% identity in 201 aa overlap); and to mycobacterial proteins e.g. O06191|Rv2621c|MTCY01A10.11 hypothetical 24.2 KDA protein from Mycobacterium tuberculosis (224 aa), FASTA scores: opt: 149, E(): 0.033, (28.05% identity in 196 aa overlap).; hypothetical protein 2947111 887690 Rv2618 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217134.1 2946434 D 83332 CDS NP_217135.1 15609756 888601 complement(2947096..2947449) 1 NC_000962.3 Rv2619c, (MTCY01A10.14), len: 117 aa. Conserved protein, highly similar to Q9L0F3|SCD31.14 hypothetical 11.6 KDA protein from Streptomyces coelicolor (110 aa),FASTA scores: opt: 407, E(): 2.3e-21, (55.95% identity in 109 aa overlap). Also similarity with other short bacterial hypothetical proteins e.g. Q9F8B9 hypothetical 12.4 KDA protein from Streptococcus agalactiae (112 aa), FASTA scores: opt: 143, E(): 0.0032, (32.45% identity in 74 aa overlap); etc.; hypothetical protein 2947449 888601 Rv2619c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217135.1 2947096 R 83332 CDS NP_217136.1 15609757 888497 complement(2947462..2947887) 1 NC_000962.3 Rv2620c, (MTCY01A10.13), len: 141 aa. Probable conserved transmembrane protein, highly similar to O54184|SC7H1.25 hypothetical 14.6 KDA protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 459,E(): 1.4e-22, (56.45% identity in 140 aa overlap). Predicted possible vaccine candidate (See Zvi et al.,2008).; Probable conserved transmembrane protein 2947887 888497 Rv2620c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217136.1 2947462 R 83332 CDS NP_217137.1 15609758 888565 complement(2947884..2948558) 1 NC_000962.3 Rv2621c, (MTCY01A10.11), len: 224 aa. Possible transcriptional regulator, similar in part to Q49688|MLCL536.29c|ML0592 putative DNA-binding protein from Mycobacterium leprae (254 aa), FASTA scores: opt: 168, E(): 0.0018, (29.75% identity in 222 aa overlap). Shows similarity with Q9XAD0|SCC22.08c putative DNA-binding protein from Streptomyces coelicolor (252 aa), FASTA scores: opt: 148, E(): 0.032, (29.4% identity in 204 aa overlap); and Q9RVM8|DR0999 conserved hypothetical protein from Deinococcus radiodurans (225 aa), FASTA scores: opt: 195, E(): 3.3e-05, (29.6% identity in 213 aa overlap). Also some similarity with O06195|Rv2618|MTCY01A10.15c from Mycobacterium tuberculosis (225 aa), FASTA scores: opt: 149, E(): 0.025, (28.95% identity in 197 aa overlap). Contains helix-turn-helix motif at aa 31-52 (Score 1662,+4.85 SD).; Possible transcriptional regulatory protein 2948558 888565 Rv2621c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_217137.1 2947884 R 83332 CDS NP_217138.1 15609759 887825 2948636..2949457 1 NC_000962.3 Rv2622, (MTCY01A10.10c), len: 273 aa. Possible methyltransferase, similar in part to others e.g. AAK75664|SP1578 putative methyltransferase from Streptococcus pneumoniae (252 aa), FASTA scores: opt: 406,E(): 6.6e-18, (32.65% identity in 251 aa overlap); Q9F8B8 methyltransferase from Streptococcus agalactiae (254 aa),FASTA scores: opt: 381, E(): 2.3e-16, (31.75% identity in 252 aa overlap); Q9RJB6|SCF91.08 putative methyltransferase from Streptomyces coelicolor (231 aa), FASTA scores: opt: 159, E(): 0.0091, (33.1% identity in 151 aa overlap); etc. Also similar in part to several hypothetical proteins e.g. Q99YR0|SPY1582 hypothetical protein from Streptococcus pyogenes (251 aa), FASTA scores: opt: 397, E(): 2.3e-17,(36.3% identity in 248 aa overlap).; Possible methyltransferase (methylase) 2949457 887825 Rv2622 Mycobacterium tuberculosis H37Rv Possible methyltransferase (methylase) NP_217138.1 2948636 D 83332 CDS NP_217139.1 15609760 887442 2949593..2950486 1 NC_000962.3 Rv2623, (MTCY01A10.09c), len: 297 aa. TB31.7,universal stress protein family protein, highly similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Q10851|YK05_MYCTU|Rv2005c|MT2061|MTCY39.12 (295 aa), FASTA scores: opt: 1076, E(): 1.4e-60, (55.25% identity in 295 aa overlap); O53472|Rv2026c|MTV018.13c (294 aa), FASTA scores: opt: 988, E(): 4.8e-55, (51.5% identity in 295 aa overlap); Q10862|YJ96_MYCTU|Rv1996|MT2052|MTCY39.23c (317 aa), FASTA scores: opt: 688, E(): 4.1e-36, (45.1% identity in 315 aa overlap); etc. Also similar to several Streptomyces proteins e.g. Q9RIZ8|SCJ1.16c conserved hypothetical protein from Streptomyces coelicolor (294 aa), FASTA scores: opt: 407, E(): 2e-18, (32.65% identity in 303 aa overlap); and other bacterial hypothetical proteins e.g. Q9HPP5|VNG1536 from Halobacterium sp (147 aa), FASTA scores: opt: 180, E(): 0.00022, (31.65% identity in 139 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). Binds ATP.; Universal stress protein family protein TB31.7 2950486 TB31.7 887442 TB31.7 Mycobacterium tuberculosis H37Rv Universal stress protein family protein TB31.7 NP_217139.1 2949593 D 83332 CDS NP_217140.1 15609761 888939 complement(2950489..2951307) 1 NC_000962.3 Rv2624c, (MTCY01A10.08), len: 272 aa. Universal stress protein family protein, similar to several Streptomyces proteins e.g. Q9RIY5|SCJ1.29c hypothetical 30.1 KDA protein from Streptomyces coelicolor (283 aa),FASTA scores: opt: 260, E(): 5e-09, (32.05% identity in 290 aa overlap). Also similar to Mycobacterium tuberculosis proteins O53474|Rv2028c|MTV018.15c (279 aa), FASTA scores: opt: 563, E(): 7e-28, (36.85% identity in 266 aa overlap); P95192|Rv3134c|MTCY03A2.240 (268 aa), FASTA scores: opt: 458, E(): 2.3e-21, (36.55% identity in 271 aa overlap); Q10851|YK05_MYCTU|Rv2005c|MT2061|MTCY39.12 (295 aa), FASTA scores: opt: 199, E(): 3.2e-05, (29.35% identity in 286 aa overlap); etc.; Universal stress protein family protein 2951307 888939 Rv2624c Mycobacterium tuberculosis H37Rv Universal stress protein family protein NP_217140.1 2950489 R 83332 CDS NP_217141.1 15609762 887692 complement(2951322..2952503) 1 NC_000962.3 Rv2625c, (MTCY01A10.07), len: 393 aa. Probable conserved transmembrane ala-, leu-rich protein, similar to many hypothetical or membrane proteins e.g. Q55518|Y528_SYNY3|SLL0528 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (379 aa),FASTA scores: opt: 552, E(): 5.6e-26, (30.75% identity in 374 aa overlap); Q9RJ56|SCI41.35c hypothetical 39.8 KDA protein from Streptomyces coelicolor (374 aa), FASTA scores: opt: 419, E(): 5.7e-18, (31.6% identity in 383 aa overlap); CAC49448|SMB20925 conserved hypothetical membrane protein from Rhizobium meliloti (Sinorhizobium meliloti) (372 aa), FASTA scores: opt: 401, E(): 6.9e-17, (29.5% identity in 383 aa overlap); etc. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature.; Probable conserved transmembrane alanine and leucine rich protein 2952503 887692 Rv2625c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane alanine and leucine rich protein NP_217141.1 2951322 R 83332 CDS NP_217142.1 15609763 888576 complement(2952562..2952993) 1 NC_000962.3 Rv2626c, (MTCY01A10.06), len: 143 aa. Hrp1, hypoxic response protein 1, similar to CAC49670|SMB21441 putative inosine-5'-monophosphate dehydrogenase protein from Rhizobium meliloti (Sinorhizobium meliloti) (120 aa), FASTA scores: opt: 287, E(): 6.6e-12, (43.75% identity in 112 aa overlap) (has its N-terminus shorter 27 aa); AAK78655|CAC0678 CBS domains from Clostridium acetobutylicum (142 aa), FASTA scores: opt: 276, E(): 3.9e-11, (35.65% identity in 115 aa overlap); Q9K9P0|BH2605 BH2605 protein from Bacillus halodurans (142 aa), FASTA scores: opt: 276, E(): 3.9e-11, (35.65% identity in 115 aa overlap); etc. Also some similarity to P71737|Rv2406c|MTCY253.14 hypothetical 15.1 KDA protein from Mycobacterium tuberculosis (142 aa), FASTA scores: opt: 145, E(): 0.00012, (22.3% identity in 112 aa overlap). Predicted possible vaccine candidate (See Zvi et al.,2008).; Hypoxic response protein 1 Hrp1 2952993 hrp1 888576 hrp1 Mycobacterium tuberculosis H37Rv Hypoxic response protein 1 Hrp1 NP_217142.1 2952562 R 83332 CDS NP_217143.1 15609764 888568 complement(2953507..2954748) 1 NC_000962.3 Rv2627c, (MTCY01A10.05), len: 413 aa. Conserved protein. Some similarity in C-terminal part of O53697|Rv0293c|MTV035.21c hypothetical 44.0 KDA protein from Mycobacterium tuberculosis (400 aa), FASTA scores: opt: 392, E(): 1.9e-17, (31.1% identity in 299 aa overlap). Alternative nucleotide at position 2954439 (T->C; R104G) has been observed. Predicted possible vaccine candidate (See Zvi et al., 2008).; hypothetical protein 2954748 888568 Rv2627c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217143.1 2953507 R 83332 CDS NP_217144.1 15609765 888566 2955058..2955420 1 NC_000962.3 Rv2628, (MTCY01A10.04c), len: 120 aa. Hypothetical unknown protein. Predicted possible vaccine candidate (See Zvi et al., 2008).; Hypothetical protein 2955420 888566 Rv2628 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217144.1 2955058 D 83332 CDS NP_217145.1 15609766 888588 2955767..2956891 1 NC_000962.3 Rv2629, (MTCY01A10.03c), len: 374 aa. Conserved protein, similar to Q9ZC00|SC1E6.22c hypothetical 40.7 KDA protein from Streptomyces coelicolor (373 aa), FASTA scores: opt: 425, E(): 2.5e-18, (30.2% identity in 371 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008).; hypothetical protein 2956891 888588 Rv2629 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217145.1 2955767 D 83332 CDS NP_217146.1 15609767 887426 2956893..2957432 1 NC_000962.3 Rv2630, (MTCY01A10.02c), len: 179 aa. Hypothetical unknown protein.; Hypothetical protein 2957432 887426 Rv2630 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217146.1 2956893 D 83332 CDS NP_217147.2 57117009 887431 2957572..2958870 1 NC_000962.3 Rv2631, (MTCY441.01, MTCY01A10.01c), len: 432 aa. Conserved hypothetical protein, highly similar to several conserved hypothetical proteins from various species e.g. O29399|AF0862 conserved hypothetical protein from Archaeoglobus fulgidus (482 aa), FASTA scores: opt: 1496,E(): 2.1e-80, (52.3% identity in 432 aa overlap) (has its N-terminus longer 30 aa); O27634|MTH1597 conserved protein from Methanothermobacter thermautotrophicus (488 aa), FASTA scores: opt: 1428, E(): 2.1e-76, (50.9% identity in 432 aa overlap); Q9YB37|APE1758 hypothetical 53.7 KDA protein APE1758 from Aeropyrum pernix (483 aa), FASTA scores: opt: 1422, E(): 4.6e-76, (49.3% identity in 432 aa overlap) (has its N-terminus longer 30 aa); etc. Equivalent to AAK47022 from Mycobacterium tuberculosis strain CDC1551 (432 aa). 3' part extended since first submission (+175 aa).; hypothetical protein 2958870 887431 Rv2631 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217147.2 2957572 D 83332 CDS NP_217148.1 15609769 887231 complement(2958909..2959190) 1 NC_000962.3 Rv2632c, (MTCY441.02c), len: 93 aa. Conserved protein, highly similar to conserved hypothetical proteins from Mycobacterium tuberculosis: P71996|YH38_MYCTU|Rv1738|MT1780|MTCY04C12.23 (94 aa), FASTA scores: opt: 319, E(): 4.2e-15, (53.95% identity in 89 aa overlap); and Q9KK61 from Mycobacterium bovis BCG (56 aa),FASTA scores: opt: 178, E(): 9.2e-06, (52.95% identity in 51 aa overlap).; hypothetical protein 2959190 887231 Rv2632c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217148.1 2958909 R 83332 CDS NP_217149.1 15609770 888597 complement(2959335..2959820) 1 NC_000962.3 Rv2633c, (MTCY441.03c), len: 161 aa. Hypothetical unknown protein.; Hypothetical protein 2959820 888597 Rv2633c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217149.1 2959335 R 83332 CDS YP_177896.1 57117010 888573 complement(2960105..2962441) 1 NC_000962.3 Rv2634c, (MTCY441.04c), len: 778 aa. PE_PGRS46,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many e.g. O53553|YZ08_MYCTU|Rv3508|MTV023.15 from Mycobacterium tuberculosis (1901 aa), FASTA scores: opt: 2553, E(): 2.2e-93, (53.8% identity in 866 aa overlap). Equivalent to AAK47026 from Mycobacterium tuberculosis strain CDC1551 (788 aa) but shorter 10 aa.; PE-PGRS family protein PE_PGRS46 2962441 PE_PGRS46 888573 PE_PGRS46 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS46 YP_177896.1 2960105 R 83332 CDS NP_217151.1 15609772 887813 2962470..2962712 1 NC_000962.3 Rv2635, (MTCY441.05), len: 80 aa. Hypothetical unknown protein.; Hypothetical protein 2962712 887813 Rv2635 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217151.1 2962470 D 83332 CDS NP_217152.1 15609773 888196 2962713..2963390 1 NC_000962.3 Rv2636, (MTCY441.06), len: 225 aa. Conserved hypothetical protein, showing some similarity with various proteins: Q98FG2|MLL3789 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (239 aa), FASTA scores: opt: 304, E(): 3.7e-13, (31.55% identity in 187 aa overlap); CAC46568|SMC04451 putative chloramphenicol phosphotransferase protein from Rhizobium meliloti (Sinorhizobium meliloti) (220 aa), FASTA scores: opt: 175,E(): 0.00014, (28.0% identity in 225 aa overlap); Q56148|CPT_STRVL chloramphenicol 3-O phosphotransferase from Streptomyces violaceus (Streptomyces venezuelae) (178 aa), FASTA scores: opt: 131, E(): 0.1, (31.75% identity in 170 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Translational start site uncertain,chosen by similarity.; hypothetical protein 2963390 888196 Rv2636 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217152.1 2962713 D 83332 CDS NP_217153.1 15609774 888616 2963586..2964242 1 NC_000962.3 Rv2637, (MTCY441.07), len: 218 aa. Possible dedA,transmembrane protein, equivalent to Q49642|YQ37_MYCLE|ML0467|MLCL581.27|B1177_C2_172/B1177_C1_ 140 hypothetical 23.1 KDA protein (potential integral membrane protein, belongs to the DedA family) from Mycobacterium leprae (214 aa), FASTA scores: opt: 1160, E(): 4.4e-64,(82.75% identity in 209 aa overlap); and O69601|Y364_MYCLE|ML0287|MLCB4.30 hypothetical protein (potential integral membrane protein) (222 aa), FASTA scores: opt: 292, E(): 6.6e-11, (32.25% identity in 189 aa overlap). Also highly similar to other membrane proteins e.g. CAC42863|SCBAC36F5.27c putative integral membrane from Streptomyces coelicolor (211 aa), FASTA scores: opt: 837,E(): 2.6e-44, (59.2% identity in 201 aa overlap); Q55705|Y232_SYNY3|SLR0232 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (218 aa),FASTA scores: opt: 415, E(): 1.9e-18, (37.85% identity in 206 aa overlap); Q9RV63|DR1167 DEDA protein from Deinococcus radiodurans (200 aa); P09548|DEDA_ECOLI|B2317|Z3579|ECS3201 DEDA protein (DSG-1 protein) from Escherichia coli strains K12 and O157:H7 (219 aa), blast scores: 178, E(): 1.8e-13, Identities = 53/175 (30%); etc. Also similar to O06314|Y364_MYCTU|Rv0364|MT0380|MTCY13E10.26 hypothetical 24.5 KDA protein (potential integral membrane protein) from Mycobacterium tuberculosis (227 aa), FASTA scores: opt: 293, E(): 5.8e-11, (35.85% identity in 184 aa overlap). Belongs to the DedA family.; Possible transmembrane protein DedA 2964242 dedA 888616 dedA Mycobacterium tuberculosis H37Rv Possible transmembrane protein DedA NP_217153.1 2963586 D 83332 CDS NP_217154.1 15609775 888156 2964405..2964851 1 NC_000962.3 Rv2638, (MTCY441.08), len: 148 aa. Conserved hypothetical protein, similar in part to Q9WVX8|RSBV_STRCO|bldg|SCH5.12c anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa), FASTA scores: opt: 162, E(): 0.00066, (31.8% identity in 110 aa overlap); and showing weak similarity with various proteins e.g. O69205 hypothetical 13.4 KDA protein from Actinosynnema pretiosum (subsp. auranticum) (128 aa), FASTA scores: opt: 157, E(): 0.0016, (29.8% identity in 114 aa overlap); Q9RJ93|SCF91.32 putative anti-sigma factor antagonist from Streptomyces coelicolor (183 aa), FASTA scores: opt: 148, E(): 0.0082, (30.85% identity in 107 aa overlap); etc. Also highly similar to hypothetical proteins from Mycobacterium tuberculosis: O07728|Rv1904|MTCY180.14c (143 aa), FASTA scores: opt: 456, E(): 3.9e-23, (52.8% identity in 125 aa overlap); and Q11035|YD65_MYCTU|Rv1365c|MT1411|MTCY02B10.29c (128 aa),FASTA scores: opt: 435, E(): 8.6e-22, (53.6% identity in 125 aa overlap).; hypothetical protein 2964851 888156 Rv2638 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217154.1 2964405 D 83332 CDS NP_217155.1 15609776 887428 complement(2965026..2965358) 1 NC_000962.3 Rv2639c, (MTCY441.09c), len: 110 aa. Probable conserved integral membrane protein, highly similar to many bacterial hypothetical or membrane proteins e.g. Q9X889|YE14_STRCO|SCE15.14 potential integral membrane protein from Streptomyces coelicolor (112 aa), FASTA scores: opt: 597, E(): 3.1e-31, (73.15% identity in 108 aa overlap); Q55939|Y793_SYNY3|SLL0793 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (108 aa), FASTA scores: opt: 341, E(): 4.9e-15, (51.4% identity in 109 aa overlap); O31553|YFJF_BACSU potential integral membrane protein from Bacillus subtilis (109 aa),FASTA scores: opt: 334, E(): 1.4e-14, (47.5% identity in 109 aa overlap); etc.; Probable conserved integral membrane protein 2965358 887428 Rv2639c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217155.1 2965026 R 83332 CDS NP_217156.1 15609777 888594 complement(2965478..2965837) 1 NC_000962.3 Rv2640c, (MTCY441.10c), len: 119 aa. Possible transcriptional regulator, arsR family, highly similar to many e.g. Q9L1V5|SC4A9.07 putative ArsR-family transcriptional regulator from Streptomyces coelicolor (117 aa), FASTA scores: opt: 261, E(): 5.6e-10, (47.75% identity in 103 aa overlap); Q9X8X8|SCH35.28c putative transcriptional regulator from Streptomyces coelicolor (122 aa), FASTA scores: opt: 252, E(): 2.2e-09, (37.05% identity in 116 aa overlap); Q9L220|SC1A2.21 putative ArsR-family transcriptional from Streptomyces coelicolor (119 aa),FASTA scores: opt: 252, E(): 2.2e-09, (37.05% identity in 116 aa overlap); P77295|YGAV_ECOLI|B2667 hypothetical transcriptional regulator from Escherichia coli strain K12 (99 aa), FASTA scores: opt: 156, E(): 0.0023, (34.1% identity in 88 aa overlap); etc. Also similar to upstream ORF P71941|Rv2642|MTCY441.12 putative transcriptional regulatory protein from Mycobacterium tuberculosis (126 aa), FASTA scores: opt: 237, E(): 2e-08, (38.55% identity in 109 aa overlap). Contains helix-turn-helix motif at aa 59-80 (Score 1166, +3.16 SD). Belongs to the ArsR family of transcriptional regulators.; Possible transcriptional regulatory protein (probably ArsR-family) 2965837 888594 Rv2640c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably ArsR-family) NP_217156.1 2965478 R 83332 CDS NP_217157.1 15609778 888629 2965939..2966397 1 NC_000962.3 Rv2641, (MTCY441.11), len: 152 aa. CadI, conserved hypothetical protein. Gene induced by cadmium (see Hotter et al., 2001), highly similar to hypothetical proteins e.g. Q9L222|SC1A2.19c from Streptomyces coelicolor (152 aa),FASTA scores: opt: 509, E(): 2.3e-27, (55.05% identity in 149 aa overlap); P45945|YQCK_BACSU from Bacillus subtilis (146 aa), FASTA scores: opt: 295, E(): 5.4e-13, (33.55% identity in 146 aa overlap); and Q98CF8|MLL5167 from Rhizobium loti (Mesorhizobium loti) (124 aa), FASTA scores: opt: 110, E(): 1.3, (31.4% identity in 121 aa overlap). Some similarity with Q10548|Y887_MYCTU|Rv0887c|MT0910|MTCY31.15c from Mycobacterium tuberculosis (152 aa), FASTA scores: opt: 108, E(): 2.1, (25.7% identity in 148 aa overlap).; Cadmium inducible protein CadI 2966397 cadI 888629 cadI Mycobacterium tuberculosis H37Rv Cadmium inducible protein CadI NP_217157.1 2965939 D 83332 CDS NP_217158.1 15609779 887703 2966533..2966913 1 NC_000962.3 Rv2642, (MTCY441.12), len: 126 aa. Possible transcriptional regulator, arsR family, highly similar to many e.g. Q9X8X8|SCH35.28c putative transcriptional regulator from Streptomyces coelicolor (122 aa), FASTA scores: opt: 390, E(): 3.7e-19, (56.55% identity in 122 aa overlap); Q9L220|SC1A2.21 putative ArsR-family transcriptional from Streptomyces coelicolor (119 aa),FASTA scores: opt: 378, E(): 2.3e-18, (59.8% identity in 97 aa overlap); Q9L1V5|SC4A9.07 putative ArsR-family transcriptional regulator from Streptomyces coelicolor (117 aa), FASTA scores: opt: 359, E(): 4.1e-17, (56.9% identity in 116 aa overlap); P52144|ARR2_ECOLI|ARSR from Escherichia coli (117 aa), FASTA scores: opt: 202, E(): 1e-06, (39.8% identity in 88 aa overlap); etc. Also similar to downstream ORF P71939|Rv2640c|MTCY441.10c putative transcriptional regulatory protein from Mycobacterium tuberculosis (119 aa), FASTA scores: opt: 237, E(): 5e-09, (38.55% identity in 109 aa overlap); and others from Mycobacterium tuberculosis e.g. O05840|Rv2358|MTCY27.22c. Contains PS00846 Bacterial regulatory proteins, arsR family signature. Contains helix-turn-helix motif at aa 58-79 (Score 1112, +2.97 SD). Belongs to the ArsR family of transcriptional regulators.; Possible transcriptional regulatory protein (probably ArsR-family) 2966913 887703 Rv2642 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably ArsR-family) NP_217158.1 2966533 D 83332 CDS NP_217159.1 15609780 887674 2966910..2968406 1 NC_000962.3 Rv2643, (MTCY441.13), len: 498 aa. Probable arsC,arsenical resistance transport integral membrane protein,highly similar or similar to others e.g. Q9L1X4|SC3D9.05 possible arsenic resistance membrane transport protein from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1729,E(): 2.2e-96, (74.3% identity in 358 aa overlap); Q9X8Y0|SCH35.26 putative heavy metal resistance membrane protein from Streptomyces coelicolor (369 aa), FASTA scores: opt: 1729, E(): 2.2e-96, (73.8% identity in 359 aa overlap); Q06598|ACR3_YEAST|ACR3|YPR201W|P9677.2 arsenical-resistance protein from Saccharomyces cerevisiae (Baker's yeast) (404 aa), FASTA scores: opt: 591, E(): 4e-28, (36.6% identity in 380 aa overlap); etc. Belongs to the ACR3 family.; Probable arsenic-transport integral membrane protein ArsC 2968406 arsC 887674 arsC Mycobacterium tuberculosis H37Rv Probable arsenic-transport integral membrane protein ArsC NP_217159.1 2966910 D 83332 CDS NP_217160.1 15609781 887366 complement(2968533..2968850) 1 NC_000962.3 Rv2644c, (MTCY441.14c), len: 105 aa. Hypothetical unknown protein.; Hypothetical protein 2968850 887366 Rv2644c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217160.1 2968533 R 83332 CDS NP_217161.1 15609782 887799 2970123..2970554 1 NC_000962.3 Rv2645, (MTCY441.15), len: 143 aa. Hypothetical unknown protein.; Hypothetical protein 2970554 887799 Rv2645 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217161.1 2970123 D 83332 CDS NP_217162.1 15609783 887706 2970551..2971549 1 NC_000962.3 Rv2646, (MTCY441.16), len: 332 aa. Probable integrase, similar to others e.g. P06723|VINT_BP186|int integrase from Bacteriophage 186 (336 aa)s FASTA scores: opt: 198, E(): 6.3e-05, (30.45% identity in 138 aa overlap). Could be belong to the 'phage' integrase family.; Probable integrase 2971549 887706 Rv2646 Mycobacterium tuberculosis H37Rv Probable integrase NP_217162.1 2970551 D 83332 CDS NP_217163.1 15609784 885870 2971659..2972027 1 NC_000962.3 Rv2647, (MTCY441.17), len: 122 aa (questionable ORF). Hypothetical protein, probably corresponds to conserved DNA sequence also found in MTCY336.29c and Rv1574|MTCY336.30c|O06616 hypothetical 11.4 KDA protein from Mycobacterium tuberculosis (103 aa), FASTA scores: opt: 170, E(): 0.0002, (69.05% identity in 42 aa overlap). Shows weak similarity with Q9EUM1|RESB resolvase protein homolog from Corynebacterium glutamicum (Brevibacterium flavum) (343 aa), FASTA scores: opt: 112, E(): 2.9, (31.05% identity in 87 aa overlap).; Hypothetical protein 2972027 885870 Rv2647 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217163.1 2971659 D 83332 CDS NP_217164.1 15609785 887828 2972160..2972486 1 NC_000962.3 Rv2648, (MTCY441.17A), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2648 and Rv2649,the sequence UUUUAAAG (directly upstream of Rv2649) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Probable transposase for insertion sequence element IS6110 (fragment) 2972486 887828 Rv2648 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS6110 (fragment) NP_217164.1 2972160 D 83332 CDS NP_217165.1 15609786 888553 <2972435..2973421 1 NC_000962.3 Rv2649, (MTCY441.18), len: 328 aa. Probable transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2648 and Rv2649, the sequence UUUUAAAG (directly upstream of Rv2649) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Probable transposase for insertion sequence element IS6110 2973421 888553 Rv2649 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS6110 NP_217165.1 <2972435 D 83332 CDS NP_217166.1 15609787 887478 complement(2973795..2975234) 1 NC_000962.3 Rv2650c, (MTCY441.19), len: 479 aa. Possible phiRv2 prophage protein (capsid subunit) (see citation below),highly similar to O06614|Rv1576c|MTCY336.28 probable phiRv1 phage protein from Mycobacterium tuberculosis (473 aa),FASTA scores: opt: 2782, E(): 2.8e-159, (89.1% identity in 468 aa overlap).; Possible PhiRv2 prophage protein 2975234 887478 Rv2650c Mycobacterium tuberculosis H37Rv Possible PhiRv2 prophage protein NP_217166.1 2973795 R 83332 CDS NP_217167.1 15609788 887837 complement(2975242..2975775) 1 NC_000962.3 Rv2651c, (MTCY441.20c), len: 177 aa. Possible protease protein, phiRv2 phage protein (prohead protease) (see citation below), showing some similarity with several proteases e.g. Q9A4P4|CC2786 putative protease from Caulobacter crescentus (138 aa), FASTA scores: opt: 206,E(): 2e-06, (36.35% identity in 132 aa overlap); Q9RNH0 putative prohead protease from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (184 aa), FASTA scores: opt: 196, E(): 1.1e-05, (35.05% identity in 137 aa overlap); BAB35014|ECS1591 putative prohead protease from Escherichia coli strain O157:H7 (185 aa), FASTA scores: opt: 187, E(): 4.1e-05, (32.9% identity in 158 aa overlap); etc. And highly similar to O06613|Rv1577c|MTCY336.27 Probable phiRV1 phage protein from Mycobacterium tuberculosis (170 aa),FASTA scores: opt: 987, E(): 2.3e-56, (89.35% identity in 169 aa overlap).; Possible PhiRv2 prophage protease 2975775 887837 Rv2651c Mycobacterium tuberculosis H37Rv Possible PhiRv2 prophage protease NP_217167.1 2975242 R 83332 CDS NP_217168.1 15609789 888577 complement(2975928..2976554) 1 NC_000962.3 Rv2652c, (MTCY441.21c), len: 208 aa. Probable phiRv2 phage protein (terminase) (see citation below), showing some similarity with AAK79859|Q97HW1|CAC1896 phage terminase-like protein (small subunit) from Clostridium acetobutylicum (151 aa), FASTA scores: opt: 155, E(): 0.012, (24.7% identity in 158 aa overlap); and Q9B019 hypothetical 17.8 KDA protein from Bacteriophage GMSE-1 (159 aa), FASTA scores: opt: 141, E(): 0.087, (27.65% identity in 159 aa overlap). Also highly similar to O06612|Rv1578c|MTCY336.26 Probable phiRV1 phage protein from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 448, E(): 1.2e-20, (48.1% identity in 156 aa overlap). Equivalent to AAK47043 from Mycobacterium tuberculosis strain CDC1551 but longer 45 aa.; Probable PhiRv2 prophage protein 2976554 888577 Rv2652c Mycobacterium tuberculosis H37Rv Probable PhiRv2 prophage protein NP_217168.1 2975928 R 83332 CDS NP_217169.1 15609790 887367 complement(2976586..2976909) 1 NC_000962.3 Rv2653c, (MTCY441.22c), len: 107 aa. Hypothetical unknown protein, possibly phiRv2 phage protein (see citation below).; Possible PhiRv2 prophage protein 2976909 887367 Rv2653c Mycobacterium tuberculosis H37Rv Possible PhiRv2 prophage protein NP_217169.1 2976586 R 83332 CDS NP_217170.1 15609791 888154 complement(2976989..2977234) 1 NC_000962.3 Rv2654c, (MTCY441.23c), len: 81 aa. Hypothetical ala-rich protein, possibly phiRv2 phage protein (see citation below), similar to C-terminus of Q9HNI3|VNG2091H hypothetical protein from Halobacterium sp. strain NRC-1 (212 aa), FASTA scores: opt: 122, E(): 0.46, (43.05% identity in 79 aa overlap).; Possible PhiRv2 prophage protein 2977234 888154 Rv2654c Mycobacterium tuberculosis H37Rv Possible PhiRv2 prophage protein NP_217170.1 2976989 R 83332 CDS NP_217171.1 15609792 887388 complement(2977231..2978658) 1 NC_000962.3 Rv2655c, (MTCY441.24c), len: 475 aa. Hypothetical protein, possibly phiRv2 phage protein (putative primase-like protein) (see citation below). C-terminus similar to P22875|YXIS_SACER hypothetical 28.9 KDA protein (probably does not play a direct role in plasmid integration or excision) from Saccharopolyspora erythraea (Streptomyces erythraeus) plasmid pSE211 (263 aa), FASTA scores: opt: 389, E(): 2.7e-15, (33.45% identity in 269 aa overlap). Weak similarity in N-terminus to O06608|MTCY336.22|Rv1582c Probable phiRV1 phage protein from Mycobacterium tuberculosis (471 aa), FASTA scores: opt: 133, E(): 2.5, (36.0% identity in 75 aa overlap).; Possible PhiRv2 prophage protein 2978658 887388 Rv2655c Mycobacterium tuberculosis H37Rv Possible PhiRv2 prophage protein NP_217171.1 2977231 R 83332 CDS NP_217172.1 15609793 888179 complement(2978660..2979052) 1 NC_000962.3 Rv2656c, (MTCY441.25c), len: 130 aa. Probable phiRv2 phage protein (see Hatfull 2000), highly similar to O06607|YF83_MYCTU|Rv1583c|MT3573.2|MTCY336.21 Probable phiRV1 phage protein from Mycobacterium tuberculosis (132 aa), FASTA scores: opt: 734, E(): 2.5e-39, (81.5% identity in 131 aa overlap); and some similarity with Q982T4|MLL8506 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (204 aa), FASTA scores: opt: 104, E(): 9.7, (31.85% identity in 113 aa overlap).; Possible PhiRv2 prophage protein 2979052 888179 Rv2656c Mycobacterium tuberculosis H37Rv Possible PhiRv2 prophage protein NP_217172.1 2978660 R 83332 CDS NP_217173.1 15609794 887399 complement(2979049..2979309) 1 NC_000962.3 Rv2657c, (MTCY441.26c), len: 86 aa. Probable phiRv2 phage protein (excisionase) (see citation below), similar to O22001|VG36_BPMD2|36|G2 gene 36 protein (GP36) from Mycobacteriophage D29 (56 aa), FASTA scores: opt: 171, E(): 9.6e-06, (48.0% identity in 50 aa overlap); and Q05246|VG36_BPML5|36 gene 36 protein (GP36) from Mycobacteriophage L5 (56 aa), FASTA scores: opt: 169, E(): 1.3e-05, (50% identity in 50 aa overlap). Similarity suggests alternative start at 21737. Contains possible helix-turn-helix motif from aa 33 to 54 (Score 1655, +4.82 SD).; Probable PhiRv2 prophage protein 2979309 887399 Rv2657c Mycobacterium tuberculosis H37Rv Probable PhiRv2 prophage protein NP_217173.1 2979049 R 83332 CDS NP_217174.1 15609795 888562 complement(2979326..2979688) 1 NC_000962.3 Rv2658c, (MTCY441.27c), len: 120 aa. Hypothetical unknown protein, probably phage protein.; Possible prophage protein 2979688 888562 Rv2658c Mycobacterium tuberculosis H37Rv Possible prophage protein NP_217174.1 2979326 R 83332 CDS NP_217175.1 15609796 885098 complement(2979691..2980818) 1 NC_000962.3 Rv2659c, (MTCY441.28c), len: 375 aa. Probable integrase, phiRv2 phage protein: putative member of the phage integrase family of tyrosine recombinases (see Hatfull 2000), highly similar to others e.g. P22884|VINT_BPML5|33|int from Mycobacteriophage L5 (371 aa), FASTA scores: opt: 836, E(): 1.2e-44, (39.0% identity in 372 aa overlap); Q38361|VINT_BPMD2|33|int from Mycobacteriophage D29 (333 aa), FASTA scores: opt: 786,E(): 1.4e-41, (40.55% identity in 338 aa overlap); etc. Seems belongs to the 'phage' integrase family.; Probable PhiRv2 prophage integrase 2980818 885098 Rv2659c Mycobacterium tuberculosis H37Rv Probable PhiRv2 prophage integrase NP_217175.1 2979691 R 83332 CDS NP_217176.1 15609797 887222 complement(2980963..2981190) 1 NC_000962.3 Rv2660c, (MTCY441.29c), len: 75 aa (questionable orf). Hypothetical unknown protein.; Hypothetical protein 2981190 887222 Rv2660c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217176.1 2980963 R 83332 CDS NP_217177.1 15609798 888545 complement(2981187..2981576) 1 NC_000962.3 Rv2661c, (MTCY441.30c), len: 129 aa. Hypothetical unknown protein.; Hypothetical protein 2981576 888545 Rv2661c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217177.1 2981187 R 83332 CDS NP_217178.1 15609799 888589 2981482..2981754 1 NC_000962.3 Rv2662, (MTCY441.31), len: 90 aa. Hypothetical unknown protein.; Hypothetical protein 2981754 888589 Rv2662 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217178.1 2981482 D 83332 CDS NP_217179.1 15609800 888561 2981853..2982086 1 NC_000962.3 Rv2663, (MTCY441.32), len: 77 aa. Hypothetical unknown protein.; Hypothetical protein 2982086 888561 Rv2663 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217179.1 2981853 D 83332 CDS NP_217180.1 15609801 888501 2982097..2982351 1 NC_000962.3 Rv2664, (MTCY441.33), len: 84 aa. Hypothetical protein. Some weak similarity to nearby P71964|Rv2667|clpX'|MT2741|MTCY441.36 possible ATP-dependent protease ATP-binding subunit from Mycobacterium tuberculosis (252 aa), FASTA scores: opt: 134, E(): 0.027, (31.15% identity in 77 aa overlap).; Hypothetical protein 2982351 888501 Rv2664 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217180.1 2982097 D 83332 CDS NP_217181.1 15609802 887748 2982699..2982980 1 NC_000962.3 Rv2665, (MTCY441.34), len: 93 aa. Hypothetical arg-rich protein, showing some similarity to N-terminus of P71640|Rv2811|MTCY16B7.32c hypothetical 21.1 KDA protein from Mycobacterium tuberculosis (202 aa), FASTA scores: opt: 157, E(): 0.0011, (37.5% identity in 72 aa overlap); and also to part of O35132|CP2B_RAT|CYP27B1|CYP27B 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial precursor from Rattus norvegicus (Rat) (501 aa), FASTA scores: opt: 106, E(): 5.4, (34.5% identity in 87 aa overlap).; Hypothetical arginine rich protein 2982980 887748 Rv2665 Mycobacterium tuberculosis H37Rv Hypothetical arginine rich protein NP_217181.1 2982699 D 83332 CDS NP_217182.1 15609803 888904 2983071..2983874 1 NC_000962.3 Rv2666, (MTCY441.35), len: 267 aa. Probable transposase (fragment), identical in region of overlap to P35882|TRA1_MYCBO|TRA1_MYCTU transposase for insertion sequence element IS1081 from Mycobacterium tuberculosis or bovis (415 aa). Last 4 codons not part of gene. Contains PS01007 Transposases, Mutator family, signature.; Probable transposase for insertion sequence element IS1081 (fragment) 2983874 888904 Rv2666 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS1081 (fragment) NP_217182.1 2983071 D 83332 CDS YP_177897.1 57117011 887415 2983896..2984654 1 NC_000962.3 Rv2667, (MTCY441.36), len: 252 aa. Possible clpC2,ATP-dependent protease atp-binding subunit, highly similar to Q9X8L2|SCE9.40 hypothetical 27.3 KDA protein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 877,E(): 2.2e-46, (57.25% identity in 255 aa overlap). The second half of the protein is highly similar to N-terminal of several CLP-family proteins e.g. P24428|CLPC_MYCLE|ML0235 probable ATP-dependent CLP protease ATP-binding subunit from Mycobacterium leprae (848 aa), FASTA scores: opt: 307, E(): 3.2e-11, (38.6% identity in 158 aa overlap); O06286|CLPC_MYCTU|Rv3596c|MT3703|MTCY07H7B.26 probable ATP-dependent CLP protease ATP-binding subunit from Mycobacterium tuberculosis (848 aa), FASTA scores: opt: 307, E(): 3.2e-11, (38.6% identity in 158 aa overlap); Q9S6T8|SCE94.24c putative CLP-family ATP-binding protease from Streptomyces coelicolor (841 aa), FASTA scores: opt: 303, E(): 5.6e-11, (38.8% identity in 152 aa overlap); etc. Some weak similarity to nearby P71961|MTCY441.33|Rv2664 hypothetical protein from Mycobacterium tuberculosis (83 aa). Contain Pfam match to entry PF02861 Clp amino terminal domain. Belongs to the CLPA/CLPB family. CLPC subfamily. Note that previously known as clpX'; Possible ATP-dependent protease ATP-binding subunit ClpC2 2984654 clpC2 887415 clpC2 Mycobacterium tuberculosis H37Rv Possible ATP-dependent protease ATP-binding subunit ClpC2 YP_177897.1 2983896 D 83332 CDS NP_217184.1 15609805 887993 2984733..2985254 1 NC_000962.3 Rv2668, (MTCY441.37), len: 173 aa. Hypothetical ala-, val-rich protein, possibly exported. Equivalent to AAK47057 from Mycobacterium tuberculosis strain CDC1551 (208 aa) but N-terminal part shorter 35 aa and with few differences. Has potential signal peptide sequence. Predicted to be an outer membrane protein (See Song et al.,2008).; Possible exported alanine and valine rich protein 2985254 887993 Rv2668 Mycobacterium tuberculosis H37Rv Possible exported alanine and valine rich protein NP_217184.1 2984733 D 83332 CDS NP_217185.1 15609806 887699 2985283..2985753 1 NC_000962.3 Rv2669, (MTCY441.38), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similarity to several proteins e.g. Q9A6M0|CC2073 acetyltransferase (GNAT family) from Caulobacter crescentus (178 aa), FASTA scores: opt: 242, E(): 1.2e-09, (30.9% identity in 165 aa overlap); Q99RQ8|SA2159 hypothetical protein similar to transcription repressor of sporulation,septation and degradation paiA from Staphylococcus aureus subsp. aureus N315 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% identity in 160 aa overlap); BAB58531|SAV2369 hypothetical 20.1 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% identity in 160 aa overlap); P21340|PAIA_BACSU|O32112 protease synthase and sporulation from Bacillus subtilis (171 aa), FASTA scores: opt: 209, E(): 2.1e-07, (22.85% identity in 162 aa overlap); etc.; GCN5-related N-acetyltransferase 2985753 887699 Rv2669 Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_217185.1 2985283 D 83332 CDS NP_217186.1 15609807 887407 complement(2985731..2986840) 1 NC_000962.3 Rv2670c, (MTCY441.39c), len: 369 aa. Conserved hypothetical protein, equivalent, but longer 164 aa, to O05683|MLC1351.22c hypothetical 17.3 KDA protein from Mycobacterium leprae (160 aa), FASTA scores: opt: 847, E(): 1.2e-45, (82.4% identity in 159 aa overlap). And highly similar to Q9X824|SC9B1.04c putative ATP/GTP-binding integral membrane protein from Streptomyces coelicolor (350 aa), FASTA scores: opt: 1169, E(): 2e-65, (56.85% identity in 343 aa overlap); and Q9RWB0|DR0759 conserved hypothetical protein from Deinococcus radiodurans (351 aa),FASTA scores: opt: 859, E(): 4e-46, (45.9% identity in 331 aa overlap). Also some similarity with other proteins e.g. P46442|YHCM_ECOLI|AAG58360|BAB37528 hypothetical protein from Escherichia coli strains K12 and O157:H7 (375 aa),FASTA scores: opt: 237, E(): 2.1e-07, (28.0% identity in 325 aa overlap); Q9JRK2|NMA1520|NMB1306 putative nucleotide-binding protein from Neisseria meningitidis (serogroup a and B) (383 aa), FASTA scores: opt: 221, E(): 2.1e-06, (27.8% identity in 356 aa overlap); Q9HVX7|PA4438 hypothetical protein from Pseudomonas aeruginosa (364 aa),FASTA scores: opt: 211, E(): 8.5e-06, (28.9% identity in 353 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; hypothetical protein 2986840 887407 Rv2670c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217186.1 2985731 R 83332 CDS NP_217187.1 15609808 887389 2986839..2987615 1 NC_000962.3 Rv2671, (MTCY441.40), len: 258 aa. Possible ribD (alternate gene name: ribG), bifunctional riboflavin biosynthesis protein incuding diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase, highly similar to O05684|MLC1351.23|ML1340 possible reductase from Mycobacterium leprae (268 aa), FASTA scores: opt: 1211,E(): 3e-68, (72.9% identity in 251 aa overlap). Also weakly similar to others e.g. Q9HWX2|RIBD|PA4056 riboflavin-specific deaminase/reductase from Pseudomonas aeruginosa (373 aa), FASTA scores: opt: 211, E(): 6.3e-06,(30.1% identity in 216 aa overlap); Q9HQA1|RIBG|VNG1256G riboflavin-specific deaminase from Halobacterium sp. strain NRC-1 (220 aa), FASTA scores: opt: 202, E(): 1.5e-05,(27.0% identity in 174 aa overlap); O28272|RIB7_ARCFU|AF2007 putative 5-amino-6-(5-phosphoribosylamino)uracil reductase (HTP reductase) from Archaeoglobus fulgidus (219 aa), FASTA scores: opt: 209, E(): 5.4e-06, (24.15% identity in 211 aa overlap); P25539|RIBD_ECOLI|RIBG|B0414 from Escherichia coli strain K12 (367 aa), FASTA scores: opt: 185, E(): 0.00026, (26.7% identity in 221 aa overlap); etc. But also similar to several hydrolases e.g. Q9X825|SC9B1.05 putative hydrolase from Streptomyces coelicolor (265 aa), FASTA scores: opt: 536, E(): 2.9e-26, (44.25% identity in 235 aa overlap); Q9RKM1|SCD17.10 putative bifunctional enzyme deaminase/reductase from Streptomyces coelicolor (376 aa),FASTA scores: opt: 228, E(): 5.6e-07, (33.5% identity in 188 aa overlap); etc. Equivalent to AAK47060 from Mycobacterium tuberculosis strain CDC1551 (239 aa) but longer 19 aa. Supposed belong to the cytidine and deoxycytidylate deaminases family in the N-terminal section; and to the HTP reductase family in the C-terminal section.; Possible bifunctional enzyme riboflavin biosynthesis protein RibD: diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino)uracil reductase (HTP reductase) 2987615 ribD 887389 ribD Mycobacterium tuberculosis H37Rv Possible bifunctional enzyme riboflavin biosynthesis protein RibD: diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino)uracil reductase (HTP reductase) NP_217187.1 2986839 D 83332 CDS NP_217188.1 15609809 887398 2987682..2989268 1 NC_000962.3 Rv2672, (MTCY441.41), len: 528 aa. Possible secreted protease, equivalent to O05685|MLC1351.24|ML1339 putative secreted protease from Mycobacterium leprae (525 aa), FASTA scores: opt: 2722, E(): 9.4e-140, (74.45% identity in 528 aa overlap). Also similar to several exported proteinases from Streptomyces and Mycobacteria e.g. Q54399|SLPE proteinase from Streptomyces lividans (513 aa), FASTA scores: opt: 429, E(): 6.8e-16, (26.2% identity in 538 aa overlap); Q9FCK9|2SC3B6.03c peptidase from Streptomyces coelicolor (513 aa), FASTA scores: opt: 421, E(): 1.8e-15,(26.45% identity in 541 aa overlap); Q10508|YM23_MYCTU from Mycobacterium tuberculosis (520 aa), FASTA scores: opt: 349, E(): 1.4e-11, (26.6% identity in 523 aa overlap); etc. Equivalent to AAK47061 from Mycobacterium tuberculosis strain CDC1551 (518 aa) but longer 10 aa. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible secreted protease 2989268 887398 Rv2672 Mycobacterium tuberculosis H37Rv Possible secreted protease NP_217188.1 2987682 D 83332 CDS NP_217189.1 15609810 887396 2989291..2990592 1 NC_000962.3 Rv2673, (MTCY441.42), len: 433 aa. Possible aftC,arabinofuranosyltransferase (See Birch et al., 2008). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Possible conserved integral membrane protein, equivalent to MLC1351.25|ML1338 possible conserved integral membrane protein from Mycobacterium leprae (440 aa), FASTA scores: opt: 2410, E(): 5.3e-143, (82.05% identity in 434 aa overlap); and showing some similarity with Q9CBX0|ML1504 probable conserved membrane protein from Mycobacterium leprae (430 aa), FASTA scores: opt: 159, E(): 0.014, (24.4% identity in 340 aa overlap). Also similar to Q53873|SC6G4.11 putative integral membrane protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 383,E(): 1.4e-16, (29.6% identity in 422 aa overlap); and with weak similarity with P71061|YVFB hypothetical protein from Bacillus subtilis (396 aa), FASTA scores: opt: 136, E(): 0.36, (24.35% identity in 279 aa overlap); and BAB60134|TVG1014811 hypothetical protein from Thermoplasma volcanium (695 aa), FASTA scores: opt: 133, E(): 0.85,(26.45% identity in 280 aa overlap). Shows also some similarity with O06557|Rv1159|MTCI65.26 hypothetical 47.1 KDA protein from Mycobacterium tuberculosis (431 aa), FASTA scores: opt: 149, E(): 0.059, (22.45% identity in 410 aa overlap); and O53515|Rv2181|MTV021.14 putative membrane protein from Mycobacterium tuberculosis (427 aa), FASTA scores: opt: 129, E(): 1, (24.8% identity in 367 aa overlap).; Possible arabinofuranosyltransferase AftC 2990592 aftC 887396 aftC Mycobacterium tuberculosis H37Rv Possible arabinofuranosyltransferase AftC NP_217189.1 2989291 D 83332 CDS NP_217190.1 15609811 886019 2990706..2991116 1 NC_000962.3 Rv2674, (MTCY441.43), len: 136 aa. Probable msrB,peptide methionine sulfoxide reductase (See Lee et al.,2008), highly similar to various proteins e.g. Q9X828|SC9B1.08 putative oxidoreductase from Streptomyces coelicolor (135 aa), FASTA scores: opt: 653, E(): 1.8e-37,(71.1% identity in 128 aa overlap); O26807|MTH711 transcriptional regulator from Methanothermobacter thermautotrophicus (151 aa), FASTA scores: opt: 533, E(): 2.7e-29, (58.15% identity in 129 aa overlap); Q9C5C8|AT4G21860 hypothetical 22.0 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (202 aa), FASTA scores: opt: 490, E(): 2.8e-26, (54.05% identity in 124 aa overlap); P39903|YEAA_ECOLI|B1778|Z2817|ECS2487 hypothetical protein from Escherichia coli strains K12 and O157:H7 (137 aa), FASTA scores: opt: 426, E(): 4.4e-22,(46.8% identity in 126 aa overlap).; Probable peptide methionine sulfoxide reductase MsrB (protein-methionine-R-oxide reductase) (peptide met(O) reductase) 2991116 msrB 886019 msrB Mycobacterium tuberculosis H37Rv Probable peptide methionine sulfoxide reductase MsrB (protein-methionine-R-oxide reductase) (peptide met(O) reductase) NP_217190.1 2990706 D 83332 CDS NP_217191.1 15609812 887760 complement(2991184..2991936) 1 NC_000962.3 Rv2675c, (MTCY441.44c), len: 250 aa. Conserved hypothetical protein. C-terminus highly similar to Q50010|U1764Z from Mycobacterium leprae (69 aa), FASTA scores: opt: 284, E(): 4.6e-11, (68.25% identity in 63 aa overlap). Shows some similarity with Q9P3V6|SPAC1348.04 (alias Q9P3E7|Q9P7U5) hypothetical 16.6 KDA protein from Schizosaccharomyces pombe (Fission yeast) (145 aa), FASTA scores: opt: 203, E(): 9.5e-06, (33.05% identity in 118 aa overlap); Q9ZSZ7|BMCT methyl chloride transferase from Batis maritima (230 aa), FASTA scores: opt: 197, E(): 3.3e-05, (28.85% identity in 156 aa overlap); P72459|STSG methyltransferase from Streptomyces griseus (253 aa), FASTA scores: opt: 194, E(): 5.5e-05, (24.45% identity in 229 aa overlap); etc. Also similar to various proteins from Mycobacterium tuberculosis e.g. P71805|Rv1377c|MTCY02B12.11c hypothetical 22.8 KDA protein (212 aa), FASTA scores: opt: 431, E(): 8.3e-20, (39.1% identity in 197 aa overlap); O06426|Rv0560c|MTCY25D10.39c hypothetical 25.9 KDA protein (241 aa), FASTA scores: opt: 379, E(): 1.6e-16, (35.95% identity in 178 aa overlap); O69667|Rv3699|MTV025.047 putative methyltransferase (233 aa), FASTA scores: opt: 297, E(): 2e-11, (30.55% identity in 193 aa overlap); etc.; hypothetical protein 2991936 887760 Rv2675c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217191.1 2991184 R 83332 CDS NP_217192.1 15609813 887718 complement(2991933..2992628) 1 NC_000962.3 Rv2676c, (MTCY441.45c), len: 231 aa. Conserved protein, equivalent to Q9CCB2|ML1045 (alias Q50009|U1764Y but longer 66 aa) hypothetical protein from Mycobacterium leprae (231 aa), FASTA scores: opt: 1401, E(): 8.7e-88,(87.45% identity in 231 aa overlap). Also highly similar to O69830|SC1B5.02 hypothetical 28.1 KDA protein from Streptomyces coelicolor (243 aa), FASTA scores: opt: 915,E(): 7.7e-55, (61.25% identity in 222 aa overlap); and similar to others e.g. Q9RUB0|DR1481 conserved hypothetical protein from Deinococcus radiodurans (289 aa), FASTA scores: opt: 327, E(): 6.1e-15, (31.8% identity in 176 aa overlap); Q97WP2|SSO2169 hypothetical protein from Sulfolobus solfataricus (223 aa), FASTA scores: opt: 285,E(): 3.4e-12, (31.3% identity in 163 aa overlap); BAB59947|TVG0805714 hypothetical protein from Thermoplasma volcanium (223 aa), FASTA scores: opt: 206, E(): 7.7e-07,(25.0% identity in 176 aa overlap); etc.; hypothetical protein 2992628 887718 Rv2676c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217192.1 2991933 R 83332 CDS YP_177675.1 57117012 887711 complement(2992634..2993992) 1 NC_000962.3 Rv2677c, (MT2751, MTV010.01c), len: 452 aa. Probable hemY, protoporphyrinogen oxidase, equivalent to Q50008|PPOX_MYCLE|HEMY|ML1044 protoporphyrinogen oxidase from Mycobacterium leprae (451 aa), FASTA scores: opt: 2211, E(): 8.8e-118, (75.4% identity in 455 aa overlap). Also similar to others e.g. Q9RV99|DR1130 from Deinococcus radiodurans (462 aa), FASTA scores: opt: 523, E(): 2.7e-22,(29.8% identity in 453 aa overlap); O32434|PPOX_PROFR|HEMY from Propionibacterium freudenreichii shermanii (527 aa),FASTA scores: opt: 344, E(): 4e-12, (32.1% identity in 495 aa overlap); P32397|PPOX_BACSU|HEMY|HEMG from Bacillus subtilis (470 aa), FASTA scores: opt: 305, E(): 5.9e-10,(26.8% identity in 463 aa overlap); etc. Belongs to the protoporphyrinogen oxidase family. Cofactor: contains one FAD per homodimer.; Probable protoporphyrinogen oxidase HemY (protoporphyrinogen-IX oxidase) (protoporphyrinogenase) (PPO) 2993992 hemY 887711 hemY Mycobacterium tuberculosis H37Rv Probable protoporphyrinogen oxidase HemY (protoporphyrinogen-IX oxidase) (protoporphyrinogenase) (PPO) YP_177675.1 2992634 R 83332 CDS NP_217194.1 15609815 888934 complement(2993989..2995062) 1 NC_000962.3 Rv2678c, (MTV010.02c), len: 357 aa. Probable hemE,uroporphyrinogen decarboxylase, equivalent to P46809|DCUP_MYCLE|heme|ML1043 uroporphyrinogen decarboxylase from Mycobacterium leprae (357 aa), FASTA scores: opt: 2017, E(): 8.2e-111, (83.75% identity in 357 aa overlap). Also highly similar to many e.g. O69861|DCUP_STRCO|heme|SC1C3.19 from Streptomyces coelicolor (355 aa), FASTA scores: opt: 1165, E(): 5.6e-61,(58.15% identity in 349 aa overlap); P32395|DCUP_BACSU|heme from Bacillus subtilis (353 aa), FASTA scores: opt: 859,E(): 4.5e-43, (44.1% identity in 356 aa overlap); Q9RV96|DCUP_DEIRA|heme|DR1133 from Deinococcus radiodurans (344 aa), FASTA scores: opt: 850, E(): 1.5e-42, (43.0% identity in 349 aa overlap); etc. Equivalent to AAK47067 from Mycobacterium tuberculosis strain CDC1551 (372 aa) but shorter 15 aa. Contains PS00907 Uroporphyrinogen decarboxylase signature 2. Belongs to the uroporphyrinogen decarboxylase family.; Probable uroporphyrinogen decarboxylase HemE (uroporphyrinogen III decarboxylase) (URO-D) (UPD) 2995062 hemE 888934 hemE Mycobacterium tuberculosis H37Rv Probable uroporphyrinogen decarboxylase HemE (uroporphyrinogen III decarboxylase) (URO-D) (UPD) NP_217194.1 2993989 R 83332 CDS NP_217195.1 15609816 887693 2995115..2995945 1 NC_000962.3 Rv2679, (MTV010.03), len: 276 aa. Probable echA15,enoyl-CoA hydratase, similar to P53526|ECHC_MYCLE|ECHA12|ML1241|MLCB1610.01|B1170_C2_224 probable enoyl-CoA hydratase from Mycobacterium leprae (294 aa), FASTA scores: opt: 368, E(): 2.5e-16, (32.15% identity in 277 aa overlap). Also highly similar to Q9RXX1|DR0184 from Deinococcus radiodurans (273 aa), FASTA scores: opt: 993, E(): 2.2e-56, (58.15% identity in 263 aa overlap); and similar to many e.g. Q9ETY7|PACA|PAAG from Azoarcus evansii (273 aa), FASTA scores: opt: 396, E(): 3.8e-18, (34.9% identity in 258 aa overlap); O29299|AF0963|FAD-3 from Archaeoglobus fulgidus (259 aa), FASTA scores: opt: 363,E(): 4.7e-16, (30.4% identity in 250 aa overlap); P77467|PAAG_ECOLI|B1394 from Escherichia coli strain W (262 aa), FASTA scores: opt: 357, E(): 1.1e-15, (31.75% identity in 252 aa overlap); etc. Also similar to O53163|ECHC_MYCTU|ECHA12|FADB2|Rv1472|MT1518|MTV007.19 enoyl-CoA hydratase from Mycobacterium tuberculosis (285 aa), FASTA scores: opt: 355, E(): 1.6e-15, (31.3% identity in 265 aa overlap); and O06542|ECHA10|Rv1142c|MTCI65.09c|Z95584 enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa). Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family.; Probable enoyl-CoA hydratase EchA15 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 2995945 echA15 887693 echA15 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA15 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_217195.1 2995115 D 83332 CDS NP_217196.1 15609817 887734 2996105..2996737 1 NC_000962.3 Rv2680, (MTV010.04), len: 210 aa. Conserved protein,equivalent to Q50005|ML1041|U1764V hypothetical protein from Mycobacterium leprae (196 aa), FASTA scores: opt: 1136, E(): 9.7e-66, (83.95% identity in 193 aa overlap). Also similar to O69860|SC1C3.18c hypothetical 24.7 KDA protein from Streptomyces coelicolor (238 aa), FASTA scores: opt: 516, E(): 5.7e-26, (45.5% identity in 189 aa overlap); and similar in part to Q9I6V4|PA0178 probable two-component sensor from Pseudomonas aeruginosa (639 aa),FASTA scores: opt: 120, E(): 3.1, (33.05% identity in 115 aa overlap); and a few other proteins. Equivalent to AAK47069 from Mycobacterium tuberculosis strain CDC1551 (178 aa) but longer 32 aa; and N-terminus highly similar to N-terminus of AAK48352|MT3984 hypothetical 4.2 KDA protein from Mycobacterium tuberculosis strain CDC1551 (38 aa),FASTA scores: opt: 102, E(): 3.6, (62.05% identity in 29 aa overlap). Nucleotide position 2996194 in the genome sequence has been corrected, T:A resulting in V30V.; hypothetical protein 2996737 887734 Rv2680 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217196.1 2996105 D 83332 CDS NP_217197.1 15609818 887708 2996739..2998055 1 NC_000962.3 Rv2681, (MTCY05A6.02), len: 438 aa. Conserved hypothetical ala-rich protein, equivalent to Q50004|ML1040|U1764U hypothetical protein from Mycobacterium leprae (429 aa), FASTA scores: opt: 2146,E(): 1.1e-119, (77.4% identity in 416 aa overlap). Also highly similar to O69858|SC1C3.16c hypothetical 42.5 KDA protein from Streptomyces coelicolor (394 aa), FASTA scores: opt: 1336, E(): 9e-72, (51.6% identity in 405 aa overlap); and with some similarity to ribonucleases D e.g. Q983F2|MLL8354 from Rhizobium loti (Mesorhizobium loti) (383 aa), FASTA scores: opt: 379, E(): 3.9e-15, (31.6% identity in 323 aa overlap); Q9A7L8|CC1704 from Caulobacter crescentus (389 aa), FASTA scores: opt: 370, E(): 1.3e-14,(31.45% identity in 318 aa overlap); CAC45770 from Rhizobium meliloti (Sinorhizobium meliloti) (383 aa), FASTA scores: opt: 331, E(): 2.7e-12, (27.75% identity in 357 aa overlap); etc.; Conserved hypothetical alanine rich protein 2998055 887708 Rv2681 Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine rich protein NP_217197.1 2996739 D 83332 CDS YP_177898.1 57117013 887461 complement(2998052..2999968) 1 NC_000962.3 Rv2682c, (MTCY05A6.03c), len: 638 aa. Probable dxs1,1-deoxy-D-xylulose 5-phosphate synthase, equivalent to Q50000|DXS_MYCLE|TKTB|ML1038 1-deoxy-D-xylulose 5-phosphate synthase from Mycobacterium leprae (643 aa), FASTA scores: opt: 3635, E(): 5.6e-209, (86.4% identity in 632 aa overlap). Also highly similar to other Q9X7W3|DXS_STRCO|DXS|SC6A5.17 from Streptomyces coelicolor (656 aa), FASTA scores: opt: 2501, E(): 2e-141, (61.3% identity in 623 aa overlap); Q9K971|DXS_BACHD|DXS|BH2779 from Bacillus halodurans (629 aa), FASTA scores: opt: 1612,E(): 1.8e-88, (41.35% identity in 619 aa overlap); P77488|DXS_ECOLI|DXS|B0420 from Escherichia coli strain K12 (619 aa), FASTA scores: opt: 1511, E(): 1.8e-82, (39.5% identity in 625 aa overlap); etc. Also similar to O50408|Rv3379c|MTV004.37c from Mycobacterium tuberculosis (536 aa). Belongs to the transketolase family. DXS subfamily. Cofactor: thiamine pyrophosphate. Note that previously known as dxs.; Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs1 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) 2999968 dxs1 887461 dxs1 Mycobacterium tuberculosis H37Rv Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs1 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) YP_177898.1 2998052 R 83332 CDS NP_217199.1 15609820 887207 3000112..3000609 1 NC_000962.3 Rv2683, (MTCY05A6.04), len: 165 aa. Conserved protein, equivalent, but shorter 19 aa, to Q49999|ML1037|U1764Q hypothetical protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 750, E(): 1.2e-41, (73.8% identity in 164 aa overlap). Shows some similarity with other hypothetical proteins e.g. Q988S9|MLL6611 from Rhizobium loti (Mesorhizobium loti) (232 aa), FASTA scores: opt: 128, E(): 0.25, (25.5% identity in 149 aa overlap); Q9YFL5|APE0233 from Aeropyrum pernix (340 aa), FASTA scores: opt: 123, E(): 0.73, (29.1% identity in 141 aa overlap); BAB60477|TVG1377730 from Thermoplasma volcanium (174 aa), FASTA scores: opt: 118,E(): 0.86, (28.8% identity in 59 aa overlap); etc.; hypothetical protein 3000609 887207 Rv2683 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217199.1 3000112 D 83332 CDS NP_217200.1 15609821 888366 3000614..3001903 1 NC_000962.3 Rv2684, (MTCY05A6.05), len: 429 aa. Probable arsA,arsenic-transport integral membrane protein, equivalent to P46838|AG45_MYCLE|ML1036 46 KDA probable integral membrane protein (antigen 45, a transmembrane protein related to arsenical pumps) from Mycobacterium leprae (429 aa), FASTA scores: opt: 2067, E(): 9.9e-118, (74.05% identity in 428 aa overlap); and upstream orf O07187|YQ85_MYCTU|ARSB|Rv2685|MT2759|MTCY05A6.06 probable integral membrane 45.2 KDA protein ARSB from Mycobacterium tuberculosis (428 aa), FASTA scores: opt: 2148, E(): 1.3e-122, (76.58% identity in 427 aa overlap). Also highly similar to other proteins e.g. Q9UY19|PAB1107 transport protein from Pyrococcus abyssi (425 aa), FASTA scores: opt: 1109, E(): 8.3e-60, (41.45% identity in 427 aa overlap); O59575|PH1912 hypothetical 46.0 KDA protein from Pyrococcus horikoshii (424 aa), FASTA scores: opt: 1101, E(): 2.5e-59,(41.95% identity in 429 aa overlap); Q9KDI2|BH1231 hypothetical 46.0 KDA protein from Bacillus halodurans (428 aa), FASTA scores: opt: 1018, E(): 2.7e-54, (38.9% identity in 427 aa overlap); etc. Belongs to the NADC/P/PHO87 family of transporters, P subfamily (ARS family).; Probable arsenic-transport integral membrane protein ArsA 3001903 arsA 888366 arsA Mycobacterium tuberculosis H37Rv Probable arsenic-transport integral membrane protein ArsA NP_217200.1 3000614 D 83332 CDS YP_177899.1 57117014 888150 3001983..3003269 1 NC_000962.3 Rv2685, (MTCY05A6.06), len: 428 aa. Probable arsB1,arsenic-transport integral membrane protein, equivalent to P46838|AG45_MYCLE|ML1036 46 KDA probable integral membrane protein (antigen 45, a transmembrane protein related to arsenical pumps) from Mycobacterium leprae (429 aa), FASTA scores: opt: 2048, E(): 7.3e-120, (74.25% identity in 427 aa overlap); and downstream ORF O07186|YQ84_MYCTU|ARSA|Rv2684|MT2758|MTCY05A6.05 probable integral membrane protein ARSA from Mycobacterium tuberculosis (429 aa), FASTA scores: opt: 2154, E(): 1.9e-126, (76.8% identity in 427 aa overlap). Also highly similar to other proteins e.g. O59575|PH1912 hypothetical 46.0 KDA protein from Pyrococcus horikoshii (424 aa), FASTA scores: opt: 1075, E(): 1.9e-59, (43.55% identity in 427 aa overlap); Q9UY19|PAB1107 transport protein from Pyrococcus abyssi (425 aa), FASTA scores: opt: 1062, E(): 1.3e-58,(41.8% identity in 428 aa overlap); Q9KDI2|BH1231 hypothetical 46.0 KDA protein from Bacillus halodurans (428 aa), FASTA scores: opt: 993, E(): 2.4e-54, (39.55% identity in 430 aa overlap); etc. Belongs to the NADC/P/PHO87 family of transporters, P subfamily. Note that previously known as arsB.; Probable arsenic-transport integral membrane protein ArsB1 3003269 arsB1 888150 arsB1 Mycobacterium tuberculosis H37Rv Probable arsenic-transport integral membrane protein ArsB1 YP_177899.1 3001983 D 83332 CDS NP_217202.1 15609823 888360 complement(3003280..3004038) 1 NC_000962.3 Rv2686c, (MTCY05A6.07c), len: 252 aa. Antibiotic-transport integral membrane leu-, ala-, val-rich protein ABC transporter (see citation below). The region from aa ~115 to 160 is highly similar to N-terminus of Q49998|U1764P hypothetical protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 151, E(): 0.011, (58.15% identity in 43 aa overlap). Shows some similarity with membrane proteins e.g. AAK75541|SP1447 membrane protein from Streptococcus pneumoniae (298 aa), FASTA scores: opt: 139, E(): 0.21, (29.65% identity in 135 aa overlap); Q9K4C9|2SC6G5.26c putative ABC transporter integral membrane subunit from Streptomyces coelicolor (249 aa),FASTA scores: opt: 138, E(): 0.21, (26.9% identity in 253 aa overlap); Q53627|MTRB membrane protein involved in mithramycin resistance from Streptomyces argillaceus (233 aa), FASTA scores: opt: 136, E(): 0.27, (26.7% identity in 191 aa overlap); etc.; Antibiotic-transport integral membrane leucine and alanine and valine rich protein ABC transporter 3004038 888360 Rv2686c Mycobacterium tuberculosis H37Rv Antibiotic-transport integral membrane leucine and alanine and valine rich protein ABC transporter NP_217202.1 3003280 R 83332 CDS NP_217203.1 15609824 888446 complement(3004035..3004748) 1 NC_000962.3 Rv2687c, (MTCY05A6.08c), len: 237 aa. Antibiotic-transport integral membrane leu-, val-rich protein ABC transporter (see citation below), showing some similarity with two other hypothetical proteins,BAB59668|TVG0517148 from Thermoplasma volcanium (241 aa),FASTA scores: opt: 136, E(): 0.32, (23.1% identity in 208 aa overlap); and Q97U55|SSO3168 from Sulfolobus solfataricus (249 aa), FASTA scores: opt: 136, E(): 0.33,(25.15% identity in 195 aa overlap). Has some hydrophobic stretches and contains bacterial regulatory proteins, araC family signature (PS00041).; Antibiotic-transport integral membrane leucine and valine rich protein ABC transporter 3004748 888446 Rv2687c Mycobacterium tuberculosis H37Rv Antibiotic-transport integral membrane leucine and valine rich protein ABC transporter NP_217203.1 3004035 R 83332 CDS NP_217204.1 15609825 888463 complement(3004745..3005650) 1 NC_000962.3 Rv2688c, (MTCY05A6.09c), len: 301 aa. Antibiotic-transport ATP-binding protein ABC transporter (see citation below), highly similar to AAK47077|MT2762 ABC transporter ATP-binding protein from Mycobacterium tuberculosis strain CDC1551 (317 aa), FASTA scores: opt: 1714, E(): 5.1e-93, (95.6% identity in 274 aa overlap). Also highly similar to other ATP-binding proteins ABC transporter e.g. Q9K639|BH3893 from Bacillus halodurans (282 aa), FASTA scores: opt: 644, E(): 1.4e-30, (38.% identity in 285 aa overlap); O58550|PH0820 from Pyrococcus horikoshii (312 aa), FASTA scores: opt: 574, E(): 1.8e-26,(39.1% identity in 307 aa overlap); Q9WYM0|TM0389 from Thermotoga maritima (301 aa), FASTA scores: opt: 536, E(): 2.9e-24, (36.1% identity in 291 aa overlap); etc. Has ATP/GTP-binding site motif A (P-loop) at N-terminus (PS00017). Belongs to the ATP-binding transport protein family (ABC transporters).; Antibiotic-transport ATP-binding protein ABC transporter 3005650 888463 Rv2688c Mycobacterium tuberculosis H37Rv Antibiotic-transport ATP-binding protein ABC transporter NP_217204.1 3004745 R 83332 CDS NP_217205.1 15609826 887219 complement(3005845..3007062) 1 NC_000962.3 Rv2689c, (MTCY05A6.10c), len: 405 aa (other less probable starts possible). Conserved ala-, val-, gly-rich protein, similar to O54099|SC10A5.06 hypothetical 49.5 KDA protein from Streptomyces coelicolor (458 aa), FASTA scores: opt: 455, E(): 2.7e-20, (38.35% identity in 417 aa overlap); and shows weak similarity in part with several methyltransferases e.g. Q9X0H9|TM1094 putative RNA methyltransferase from Thermotoga maritima (439 aa), FASTA scores: opt: 306, E(): 3e-11, (25.9% identity in 436 aa overlap); AK79403|CAC1435 S-adenosylmethionine-dependent methyltransferases from Clostridium acetobutylicum (456 aa), FASTA scores: opt: 294, E(): 1.6e-10, (23.4% identity in 449 aa overlap); Q9A8M7|CC1326 RNA methyltransferase from Caulobacter crescentus (415 aa), FASTA scores: opt: 247, E(): 1.1e-07, (28.4% identity in 433 aa overlap); etc. Equivalent to AAK47078 from Mycobacterium tuberculosis strain CDC1551 (434 aa) but shorter 29 aa.; Conserved alanine and valine and glycine rich protein 3007062 887219 Rv2689c Mycobacterium tuberculosis H37Rv Conserved alanine and valine and glycine rich protein NP_217205.1 3005845 R 83332 CDS NP_217206.1 15609827 888011 complement(3007236..3009209) 1 NC_000962.3 Rv2690c, (MTCY05A6.11c), len: 657 aa. Probable conserved integral membrane ala-, val-, leu-rich protein,highly similar to others e.g. O54098|SC10A5.05 putative membrane protein from Streptomyces coelicolor (691 aa),FASTA scores: opt: 2007, E(): 1.6e-116, (62.35% identity in 669 aa overlap); O69917|SC3C8.04c putative integral membrane protein from Streptomyces coelicolor (644 aa),FASTA scores: opt: 923, E(): 1.7e-49, (35.3% identity in 669 aa overlap); AAK78253|CAC0272 amino acid transporter from Clostridium acetobutylicum (620 aa), FASTA scores: opt: 674, E(): 4.1e-34, (36.55% identity in 640 aa overlap); etc.; Probable conserved integral membrane alanine and valine and leucine rich protein 3009209 888011 Rv2690c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane alanine and valine and leucine rich protein NP_217206.1 3007236 R 83332 CDS YP_177900.1 57117015 887250 3009344..3010027 1 NC_000962.3 Rv2691, (MTCY05A6.12), len: 227 aa. CeoB (alternate gene name: trkA), TRK system potassium uptake protein (see citation below), highly similar to others e.g. Q53949|TRKA_STRCO|SC2E9.17c from Streptomyces coelicolor (223 aa), FASTA scores: opt: 781, E(): 5.8e-42, (53.2% identity in 220 aa overlap); O27333|TRKA_METTH|MTH1265 from Methanobacterium thermoautotrophicum (216 aa), FASTA scores: opt: 287, E(): 5.3e-11, (27.0% identity in 211 aa overlap); O54141|SC2E9.16c from Streptomyces coelicolor (226 aa), FASTA scores: opt: 269, E(): 7.3e-10, (29.9% identity in 214 aa overlap); etc. Also similar to upstream orf O07194|CEOC|TRKA_MYCTU|TRKA|TRKB|Rv2692|MT2766|MTCY05A6.13 TRK system potassium uptake protein from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 259, E(): 3e-09,(26.55% identity in 226 aa overlap). Contains a motif common to NAD+ binding pockets (see citation below). Belongs to the TrkA family.; TRK system potassium uptake protein CeoB 3010027 ceoB 887250 ceoB Mycobacterium tuberculosis H37Rv TRK system potassium uptake protein CeoB YP_177900.1 3009344 D 83332 CDS YP_177901.1 57117016 887493 3010024..3010686 1 NC_000962.3 Rv2692, (MTCY05A6.13), len: 220 aa. CeoC (alternate gene names: trkA and trkB), TRK system potassium uptake protein (see citation below), highly similar to others e.g. O54141|SC2E9.16c from Streptomyces coelicolor (226 aa),FASTA scores: opt: 870, E(): 9.4e-48, (58.8% identity in 216 aa overlap); Q58505|TRKA_METJA|MJ1105 from Methanococcus jannaschii (218 aa), FASTA scores: opt: 361,E(): 9.7e-16, (29.8% identity in 218 aa overlap); O27333|TRKA_METTH|MTH1265 from Methanobacterium thermoautotrophicum (216 aa), FASTA scores: opt: 326, E(): 1.5e-13, (30.1% identity in 216 aa overlap); etc. Also similar to downstream orf O07193|CEOB|TRKA|Rv2691|MTCY05A6.12 TRK system potassium uptake protein from Mycobacterium tuberculosis (227 aa),FASTA scores: opt: 259, E(): 2.6e-09, (26.55% identity in 226 aa overlap). Contains a motif common to NAD+ binding pockets (see citation below). Belongs to the TrkA family.; TRK system potassium uptake protein CeoC 3010686 ceoC 887493 ceoC Mycobacterium tuberculosis H37Rv TRK system potassium uptake protein CeoC YP_177901.1 3010024 D 83332 CDS NP_217209.1 15609830 887917 complement(3010697..3011368) 1 NC_000962.3 Rv2693c, (MTCY05A6.14c), len: 223 aa. Probable conserved integral membrane ala-, leu-rich protein, showing some similarity to O54140|SC2E9.15 hypothetical 29.6 KDA protein from Streptomyces coelicolor (272 aa), FASTA scores: opt: 212, E(): 4.3e-06, (23.5% identity in 247 aa overlap).; Probable conserved integral membrane alanine and leucine rich protein 3011368 887917 Rv2693c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane alanine and leucine rich protein NP_217209.1 3010697 R 83332 CDS NP_217210.1 15609831 888911 complement(3011399..3011767) 1 NC_000962.3 Rv2694c, (MTCY05A6.15c), len: 122 aa. Conserved protein, highly similar in part to SC2E9.14 hypothetical 16.9 KDA protein from Streptomyces coelicolor (154 aa),FASTA scores: opt: 299, E(): 1.9e-13, (41.05% identity in 117 aa overlap. Equivalent to AAK47083 from Mycobacterium tuberculosis strain CDC1551 (157 aa) but shorter 35 aa.; hypothetical protein 3011767 888911 Rv2694c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217210.1 3011399 R 83332 CDS NP_217211.1 15609832 888223 3011916..3012623 1 NC_000962.3 Rv2695, (MTCY05A6.16), len: 235 aa. Conserved hypothetical ala-rich protein, equivalent to Q49994|ML1030|U1764L hypothetical protein from Mycobacterium leprae (232 aa), FASTA scores: opt: 1166,E(): 6.3e-63, (76.95% identity in 230 aa overlap). Also shows some similarity with other hypothetical proteins e.g. Q986S2|MLR7232 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (277 aa), FASTA scores: opt: 150, E(): 0.059, (33.55% identity in 173 aa overlap); CAC47772|SMC03810 hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (269 aa), FASTA scores: opt: 143, E(): 0.15, (28.05% identity in 228 aa overlap); Q9A5N6|CC2411 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Caulobacter crescentus (393 aa), FASTA scores: opt: 138,E(): 0.41, (26.45% identity in 238 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Nucleotide position 3012293 in the genome sequence has been corrected, A:G resulting in T126T.; Conserved hypothetical alanine rich protein 3012623 888223 Rv2695 Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine rich protein NP_217211.1 3011916 D 83332 CDS NP_217212.1 15609833 888482 complement(3012829..3013608) 1 NC_000962.3 Rv2696c, (MTCY05A6.17c), len: 259 aa. Conserved ala-, gly-, val-rich protein, equivalent (but shorter 18 aa) to Q49993|ML1029|U1764K hypothetical protein from Mycobacterium leprae (273 aa), FASTA scores: opt: 1174,E(): 2.1e-63, (70.6% identity in 262 aa overlap). Also similar to O54135|SC2E9.10 from Streptomyces coelicolor (250 aa), FASTA scores: opt: 213, E(): 9.8e-06, (28.25% identity in 255 aa overlap); and showing weak similarity with other proteins.; Conserved alanine and glycine and valine rich protein 3013608 888482 Rv2696c Mycobacterium tuberculosis H37Rv Conserved alanine and glycine and valine rich protein NP_217212.1 3012829 R 83332 CDS NP_217213.1 15609834 887290 complement(3013683..3014147) 1 NC_000962.3 Rv2697c, (MT2771, MTCY05A6.18c), len: 154 aa. Probable dut, deoxyuridine 5'-triphosphate nucleotidohydrolase (see citation below), equivalent to Q49992|DUT_MYCLE|ML1028 deoxyuridine 5'-triphosphate nucleotidohydrolase from Mycobacterium leprae (154 aa),FASTA scores: opt: 928, E(): 2.1e-51, (90.25% identity in 154 aa overlap). Also highly similar to others e.g. O54134|DUT_STRCO|SC2E9.09 from Streptomyces coelicolor (183 aa), FASTA scores: opt: 534, E(): 1.2e-26, (56.1% identity in 148 aa overlap); O66592|DUT_AQUAE|AQ_220 from Aquifex aeolicus (150 aa), FASTA scores: opt: 398, E(): 3.3e-18,(48.05% identity in 152 aa overlap); Q9X3X5|DUT_ZYMMO from Zymomonas mobilis (146 aa), FASTA scores: opt: 396, E(): 4.4e-18, (49.0% identity in 147 aa overlap); etc. Belongs to the dUTPase family.; Probable deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (dUTPase) (dUTP pyrophosphatase) (deoxyuridine 5'-triphosphatase) (dUTP diphosphatase) (deoxyuridine-triphosphatase) 3014147 dut 887290 dut Mycobacterium tuberculosis H37Rv Probable deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (dUTPase) (dUTP pyrophosphatase) (deoxyuridine 5'-triphosphatase) (dUTP diphosphatase) (deoxyuridine-triphosphatase) NP_217213.1 3013683 R 83332 CDS NP_217214.1 15609835 888528 3014173..3014658 1 NC_000962.3 Rv2698, (MTCY05A6.19), len: 161 aa. Probable conserved ala-rich transmembrane protein, equivalent to Q49991|ML1027|U1764I possible membrane protein from Mycobacterium leprae (157 aa), FASTA scores: opt: 886, E(): 1.1e-49, (78.9% identity in 161 aa overlap). Also similar to O54132|SC2E9.07c hypothetical 16.5 KDA protein from Streptomyces coelicolor (154 aa), FASTA scores: opt: 230,E(): 7.1e-08, (35.7% identity in 154 aa overlap).; Probable conserved alanine rich transmembrane protein 3014658 888528 Rv2698 Mycobacterium tuberculosis H37Rv Probable conserved alanine rich transmembrane protein NP_217214.1 3014173 D 83332 CDS NP_217215.1 15609836 887218 complement(3014663..3014965) 1 NC_000962.3 Rv2699c, (MTCY05A6.20c), len: 100 aa. Conserved hypothetical protein, very equivalent to Q49990|ML1026|U1764J hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 632, E(): 7.7e-36, (96.0% identity in 100 aa overlap). Also highly similar to O54130|SC2E9.05 hypothetical 11.0 KDA protein from Streptomyces coelicolor (98 aa), FASTA scores: opt: 465, E(): 1.1e-24, (71.45% identity in 98 aa overlap).; hypothetical protein 3014965 887218 Rv2699c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217215.1 3014663 R 83332 CDS NP_217216.1 15609837 887405 3015203..3015853 1 NC_000962.3 Rv2700, (MTCY05A6.21), len: 216 aa. Possible secreted ala-rich protein, equivalent to Q4998|ML1025|U1764H possible secreted protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1198,E(): 1.2e-65, (82.4% identity in 216 aa overlap). Also showing some similarity with Q9AK75|2SCD60.08c conserved hypothetical protein from Streptomyces coelicolor (204 aa),FASTA scores: opt: 193, E(): 8.9e-05, (31.25% identity in 192 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved secreted alanine rich protein 3015853 887405 Rv2700 Mycobacterium tuberculosis H37Rv Possible conserved secreted alanine rich protein NP_217216.1 3015203 D 83332 CDS NP_217217.1 15609838 887210 complement(3015863..3016735) 1 NC_000962.3 Rv2701c, (MTCY05A6.22c), len: 290 aa. SuhB,inositol-1-monophosphatase. Equivalent to AAK47090 from Mycobacterium tuberculosis strain CDC1551 (277 aa) but longer 13 aa. Contains PS00630 Inositol monophosphatase family signatures 1 and 2 (PS00629 and PS00630). Belongs to the inositol monophosphatase family. Cofactor: Mg2+. Activity is inhibited by Li+ but not when Leu81 is mutated (See Nigou et al., 2002). Mg2+ promotes dimerization; Li+ amplifies this effect but does not promote dimerization on its own (See Brown et al., 2007).; Inositol-1-monophosphatase SuhB 3016735 suhB 887210 suhB Mycobacterium tuberculosis H37Rv Inositol-1-monophosphatase SuhB NP_217217.1 3015863 R 83332 CDS NP_217218.1 15609839 887313 3016858..3017655 1 NC_000962.3 Rv2702, (MTCY05A6.23), len: 265 aa. PpgK,polyphosphate glucokinase (see citations below),equivalent, but shorter 60 aa, to Q49988|PPGK_MYCLE|ML1023|U1764FG polyphosphate glucokinase from Mycobacterium leprae (324 aa), FASTA scores: opt: 1411, E(): 5.6e-80, (82.8% identity in 262 aa overlap). Also highly similar (or just similar) to others e.g. Q9ADE8|PPGK from Streptomyces coelicolor (246 aa), FASTA scores: opt: 912, E(): 3e-49, (57.3% identity in 239 aa overlap); Q9AGV8|PPGK from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (277 aa), FASTA scores: opt: 890, E(): 7.5e-48, (57.75% identity in 239 aa overlap); P40184|GLK_STRCO|SC6E10.20c from Streptomyces coelicolor (317 aa), FASTA scores: opt: 233, E(): 3.2e-07, (31.3% identity in 163 aa overlap); etc.; Polyphosphate glucokinase PpgK (polyphosphate-glucose phosphotransferase) 3017655 ppgK 887313 ppgK Mycobacterium tuberculosis H37Rv Polyphosphate glucokinase PpgK (polyphosphate-glucose phosphotransferase) NP_217218.1 3016858 D 83332 CDS NP_217219.1 15609840 887477 3017835..3019421 1 NC_000962.3 Rv2703, (MTCY05A6.24), len: 528 aa. SigA (formerly named mysA, and also known as rpoV or rpoD), RNA polymerase sigma factor (see citations below), equivalent (but shorter 55 aa) to Q9S5K3|RPOT (alias Q59532) RNA polymerase sigma factor from Mycobacterium leprae (576 aa), FASTA scores: opt: 2638, E(): 8.6e-115, (80.35% identity in 535 aa overlap). Also similar to others e.g. Q59552|MYSA from Mycobacterium smegmatis (466 aa), FASTA scores: opt: 2259,E(): 2.3e-97, (76.5% identity in 528 aa overlap); Q45302|SIGA from Corynebacterium glutamicum (Brevibacterium flavum) (497 aa), FASTA scores: opt: 1972, E(): 4.3e-84,(67.35% identity in 505 aa overlap); Q59813|HRDB from Streptomyces aureofaciens (525 aa), FASTA scores: opt: 1654, E(): 2.1e-69, (67.5% identity in 468 aa overlap); etc. Contains sigma-70 family signatures 1 and 2 (PS00715 and PS00716). Belongs to the sigma-70 factor family.; RNA polymerase sigma factor SigA (sigma-A) 3019421 sigA 887477 sigA Mycobacterium tuberculosis H37Rv RNA polymerase sigma factor SigA (sigma-A) NP_217219.1 3017835 D 83332 CDS NP_217220.1 15609841 887675 3019458..3019886 1 NC_000962.3 Rv2704, (MTCY05A6.25), len: 142 aa. Conserved protein, highly similar (but shorter 25 aa) to Q9RYB7|DR0033 conserved hypothetical protein from Deinococcus radiodurans (157 aa), FASTA scores: opt: 381,E(): 1.5e-17, (54.85% identity in 124 aa overlap); and highly similar to various proteins e.g. CAC47758|SMC03796 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (126 aa), FASTA scores: opt: 302,E(): 1.4e-12, (46.6% identity in 126 aa overlap); Q98E55|MLL4402 from Rhizobium loti (Mesorhizobium loti) (130 aa), FASTA scores: opt: 252, E(): 2.1e-09, (40.15% identity in 127 aa overlap); Q9K3V5|SCD10.21 putative acetyltransferase from Streptomyces coelicolor (291 aa),FASTA scores: opt: 247, E(): 8.7e-09, (41.3% identity in 138 aa overlap) (homology only in N-terminal region); etc. Belongs to the YJGF/YER057C/UK114 protein family.; hypothetical protein 3019886 887675 Rv2704 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217220.1 3019458 D 83332 CDS NP_217221.1 15609842 887302 complement(3019814..3020203) 1 NC_000962.3 Rv2705c, (MTCY05A6.26c), len: 129 aa (unlikely ORF). Conserved hypothetical protein, similar to others e.g. Q9RXR5|DR0242 conserved hypothetical protein from Deinococcus radiodurans (112 aa), FASTA scores: opt: 259,E(): 9.4e-10, (40.5% identity in 116 aa overlap); CAC45122|SMC02246 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (115 aa), FASTA scores: opt: 208, E(): 1.6e-06, (38.3% identity in 107 aa overlap); Q98B88|MLL5682 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (116 aa), FASTA scores: opt: 173, E(): 0.00026, (34.95% identity in 103 aa overlap); etc.; hypothetical protein 3020203 887302 Rv2705c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217221.1 3019814 R 83332 CDS NP_217222.1 15609843 887484 complement(3020200..3020457) 1 NC_000962.3 Rv2706c, (MTCY05A6.27c), len: 85 aa (unlikely ORF). Hypothetical unknown protein.; Hypothetical protein 3020457 887484 Rv2706c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217222.1 3020200 R 83332 CDS NP_217223.1 15609844 887750 3020573..3021547 1 NC_000962.3 Rv2707, (MTCY05A6.28), len: 324 aa. Probable conserved transmembrane ala-, leu-rich protein, equivalent to Q49985|ML1017|U1764D possible conserved integral membrane protein from Mycobacterium leprae (330 aa), FASTA scores: opt: 1617, E(): 2.5e-91, (75.4% identity in 325 aa overlap). Also similar to other membrane proteins e.g. Q9ADF6|SCBAC1A6.31 putative integral membrane protein from Streptomyces coelicolor (344 aa), FASTA scores: opt: 593,E(): 5.9e-29, (36.2% identity in 268 aa overlap); Q99SZ8|SA1699 hypothetical protein (similar to transporter) from Staphylococcus aureus subsp. aureus N315 (405 aa),FASTA scores: opt: 318, E(): 3.7e-12, (27.9% identity in 265 aa overlap); O34437|YFKH hypothetical protein (similar to transporter) from Bacillus subtilis (275 aa), FASTA scores: opt: 309, E(): 9.7e-12, (29.3% identity in 263 aa overlap); etc.; Probable conserved transmembrane alanine and leucine rich protein 3021547 887750 Rv2707 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane alanine and leucine rich protein NP_217223.1 3020573 D 83332 CDS NP_217224.1 15609845 887159 complement(3021548..3021796) 1 NC_000962.3 Rv2708c, (MTCY05A6.29), len: 82 aa. Conserved hypothetical protein, equivalent (but shorter 25 aa) to Q49984|ML1016|U1764C hypothetical protein from Mycobacterium leprae (107 aa), FASTA scores: opt: 492, E(): 7.3e-27, (87.8% identity in 82 aa overlap). Also highly similar to Q9L1U7|SCE59.06c hypothetical 10.4 KDA protein from Streptomyces coelicolor (97 aa), FASTA scores: opt: 200, E(): 4.4e-07, (51.6% identity in 62 aa overlap).; hypothetical protein 3021796 887159 Rv2708c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217224.1 3021548 R 83332 CDS NP_217225.1 15609846 887282 3021839..3022285 1 NC_000962.3 Rv2709, (MTCY05A6.30), len: 148 aa. Probable conserved transmembrane protein, equivalent to Q9CCB4|ML1015 (alias Q49983|U1764B but extended in N-terminus) possible conserved membrane protein from Mycobacterium leprae (139 aa), FASTA scores: opt: 578, E(): 5.5e-31, (70.75% identity in 123 aa overlap). Shows also similarity with Q9RJ48|SCI8.05 putative integral membrane protein from Streptomyces coelicolor (159 aa), FASTA scores: opt: 119, E(): 0.57, (31.95% identity in 119 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 3022285 887282 Rv2709 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217225.1 3021839 D 83332 CDS NP_217226.1 15609847 888580 3022461..3023432 1 NC_000962.3 Rv2710, (MTCY05A6.31), len: 323 aa. SigB (formerly known as mysB), RNA polymerase sigma factor (see citations below), equivalent to Q59531|ML1014 RNA polymerase sigma factor from Mycobacterium leprae (319 aa), FASTA scores: opt: 1935, E(): 1.9e-109, (96.2% identity in 316 aa overlap). Also highly similar to others e.g. Q59553|MYSB from Mycobacterium smegmatis (319 aa), FASTA scores: opt: 1874, E(): 9.1e-106, (92.4% identity in 316 aa overlap); Q9ANT6|SIGB from Brevibacterium flavum (331 aa), FASTA scores: opt: 1525, E(): 9.9e-85, (78.9% identity in 303 aa overlap); Q60158|RPOV from Mycobacterium bovis (528 aa),FASTA scores: opt: 1246, E(): 9.3e-68, (62.85% identity in 315 aa overlap); etc. Contains sigma-70 factors family signatures 1 and 2 (PS00715 and PS00716). And contains possible helix-turn-helix motif at aa 282-303 (Score 1887,+5.61 SD). Belongs to the sigma-70 factor family.; RNA polymerase sigma factor SigB 3023432 sigB 888580 sigB Mycobacterium tuberculosis H37Rv RNA polymerase sigma factor SigB NP_217226.1 3022461 D 83332 CDS NP_217227.1 15609848 888590 3023565..3024257 1 NC_000962.3 Rv2711, (MTCY05A6.32), len: 230 aa. IdeR (formerly known as dtxR), iron dependent repressor and activator (see citations below), equivalent to Q9CCB5|ML1013 iron dependent repressor from Mycobacterium leprae (230 aa),FASTA scores: opt: 1365, E(): 3.8e-77, (90.0% identity in 230 aa overlap). Also highly similar to others e.g. Q50379|DTXR from Mycobacterium smegmatis (233 aa), FASTA scores: opt: 1291, E(): 1.4e-72, (86.1% identity in 230 aa overlap); Q9F7T3|IDER from Corynebacterium equii (Rhodococcus equi) (230 aa), FASTA scores: opt: 1130, E(): 1.2e-62, (74.8% identity in 230 aa overlap); P33120|DTXR_CORDI from Corynebacterium diphtheriae (226 aa), FASTA scores: opt: 803, E(): 1.6e-42, (57.85% identity in 230 aa overlap); etc. Belongs to the fur family.; Iron-dependent repressor and activator IdeR 3024257 ideR 888590 ideR Mycobacterium tuberculosis H37Rv Iron-dependent repressor and activator IdeR NP_217227.1 3023565 D 83332 CDS NP_217228.1 15609849 888586 complement(3024270..3025328) 1 NC_000962.3 Rv2712c, (MTCY05A6.33c), len: 352 aa. Hypothetical unknown ala-, leu-rich protein.; Hypothetical protein 3025328 888586 Rv2712c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217228.1 3024270 R 83332 CDS NP_217229.1 15609850 887355 3025441..3026847 1 NC_000962.3 Rv2713, (MT2786, MTCY05A6.34), len: 468 aa. Probable sthA, soluble pyridine nucleotide transhydrogenase, highly similar to others e.g. Q983E2|MLR8366 from Rhizobium loti (Mesorhizobium loti) (481 aa), FASTA scores: opt: 1447,E(): 4.1e-78, (49.55% identity in 460 aa overlap); P27306|STHA_ECOLI|STH|UDHA|B3962 from Escherichia coli strain K12 (465 aa), FASTA scores: opt: 1267, E(): 1.7e-67,(43.05% identity in 462 aa overlap); O05139|STHA_PSEFL|STH from Pseudomonas fluorescens (463 aa), FASTA scores: opt: 1257, E(): 6.6e-67, (43.8% identity in 461 aa overlap); etc. Also highly similar to CAC46308|SMC00300 putative oxidoreductase protein from Rhizobium meliloti (Sinorhizobium meliloti) (467 aa), FASTA scores: opt: 1466,E(): 3e-79, (49.55% identity in 462 aa overlap). Shows some similarity to MTCY359.04, E(): 3.1e-08; MTCY210.05, E(): 3.4e-08. Contains ATP/GTP-binding site motif A (P-loop; PS00017). Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Cofactor: FAD (by similarity).; Probable soluble pyridine nucleotide transhydrogenase SthA (STH) (NAD(P)(+) transhydrogenase [B-specific]) (nicotinamide nucleotide transhydrogenase) 3026847 sthA 887355 sthA Mycobacterium tuberculosis H37Rv Probable soluble pyridine nucleotide transhydrogenase SthA (STH) (NAD(P)(+) transhydrogenase [B-specific]) (nicotinamide nucleotide transhydrogenase) NP_217229.1 3025441 D 83332 CDS NP_217230.1 15609851 887653 3027065..3028039 1 NC_000962.3 Rv2714, (MTCY05A6.35), len: 324 aa. Conserved ala-,leu-rich protein, equivalent to Q49847|ML1009|B2235_F1_6 hypothetical protein from Mycobacterium leprae (326 aa),FASTA scores: opt: 1881, E(): 5.8e-107, (89.7% identity in 320 aa overlap); and similar to Q49797|MLCB2533.03c|B2126_F1_36 hypothetical protein from Mycobacterium leprae (317 aa), FASTA scores: opt: 376, E(): 1.2e-15, (30.1% identity in 279 aa overlap); and Q9CC38|ML1306 hypothetical protein from Mycobacterium leprae (274 aa), FASTA scores: opt: 367, E(): 3.6e-15,(29.8% identity in 275 aa overlap). Also highly similar to Q9S2K6|SC7H2.11c hypothetical 34.2 KDA protein from Streptomyces coelicolor (312 aa), FASTA scores: opt: 770,E(): 1.4e-39, (40.9% identity in 286 aa overlap); and similar to Q9ADA5|SCI52.04 conserved hypothetical protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 386, E(): 3e-16, (29.05% identity in 296 aa overlap). Also similar to O33260|Rv2125|MTCY261.21 hypothetical protein from Mycobacterium tuberculosis (292 aa), FASTA scores: opt: 387, E(): 2.3e-16, (29.45% identity in 292 aa overlap).; Conserved alanine and leucine rich protein 3028039 887653 Rv2714 Mycobacterium tuberculosis H37Rv Conserved alanine and leucine rich protein NP_217230.1 3027065 D 83332 CDS NP_217231.1 15609852 887974 3028098..3029123 1 NC_000962.3 Rv2715, (MTCY05A6.36), len: 341 aa. Possible hydrolase, showing some similarity with other hydrolases e.g. Q9I5B0|PA0829 probable hydrolase from Pseudomonas aeruginosa (313 aa), FASTA scores: opt: 336, E(): 9.9e-14,(28.05% identity in 289 aa overlap); BAB55888 hydrolase (fragment) from Terrabacter sp. DBF63 (319 aa), FASTA scores: opt: 326, E(): 4.2e-13, (27.95% identity in 290 aa overlap); O52866|CEH|eh soluble epoxide hydrolase from Corynebacterium SP (285 aa), FASTA scores: opt: 325, E(): 4.4e-13, (29.95% identity in 284 aa overlap); etc. Also shows some similarity to P96811|EPHF|Rv0134|MTCI5.08 hypothetical 33.8 KDA protein from Mycobacterium tuberculosis (300 aa), FASTA scores: E(): 1.8e-10, (27.7% identity in 271 aa overlap). Contains lipases, serine active site motif (PS00120).; Possible hydrolase 3029123 887974 Rv2715 Mycobacterium tuberculosis H37Rv Possible hydrolase NP_217231.1 3028098 D 83332 CDS NP_217232.1 15609853 887775 3029172..3029858 1 NC_000962.3 Rv2716, (MTCY05A6.37), len: 228 aa. Conserved protein, similar to other proteins e.g. Q9RKR0|SCC75A.14 hypothetical 23.3 KDA protein from Streptomyces coelicolor (214 aa), FASTA scores: opt: 447, E(): 4e-22, (44.1% identity in 220 aa overlap); Q9HHG6|PHZF|VNG6408G phenazine biosynthetic protein from Halobacterium sp. strain NRC-1 (299 aa), FASTA scores: opt: 201, E(): 6.1e-06, (30.4% identity in 148 aa overlap) (similarity only at N-terminus); P73125|SLR1019 hypothetical 34.1 KDA protein from Synechocystis sp. strain PCC 6803 (314 aa), FASTA scores: opt: 196, E(): 1.4e-05, (28.5% identity in 298 aa overlap); etc.; hypothetical protein 3029858 887775 Rv2716 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217232.1 3029172 D 83332 CDS NP_217233.1 15609854 887284 complement(3029867..3030361) 1 NC_000962.3 Rv2717c, (MTCY05A6.38c), len: 164 aa. Conserved protein, equivalent to Q9CCB8|ML1006 (alias Q49838 but shortened N-terminus) hypothetical protein from Mycobacterium leprae (161 aa), FASTA scores: opt: 797, E(): 2.3e-46, (73.8% identity in 164 aa overlap). Also highly similar to other eukaryotic proteins e.g. O64527|YUP8H12R.14 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (166 aa), FASTA scores: opt: 393, E(): 2.3e-19, (42.4% identity in 158 aa overlap); Q9Y325 CGI-36 protein from Homo sapiens (Human) (165 aa),FASTA scores: opt: 294, E(): 9.5e-13, (33.95% identity in 159 aa overlap); etc.; hypothetical protein 3030361 887284 Rv2717c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217233.1 3029867 R 83332 CDS NP_217234.1 15609855 887985 complement(3030413..3030877) 1 NC_000962.3 Rv2718c, (MTCY05A6.39c), len: 154 aa. Probable nrdR,transcriptional regulatory protein, equivalent to Q49844|ML1005|U2235A|B2235_C2_209 hypothetical 17.3 KDA protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 937, E(): 1.5e-52, (92.7% identity in 151 aa overlap). Highly similar to O86848|NRDR_STRCL putative regulatory protein from Streptomyces clavuligerus (172 aa), FASTA scores: opt: 750, E(): 1.1e-40, (73.65% identity in 148 aa overlap); O69980|SC4H2.25 hypothetical protein from Streptomyces coelicolor (182 aa), FASTA scores: opt: 725,E(): 4.6e-39, (73.1% identity in 145 aa overlap); Q9KPU0|VC2272 hypothetical protein from Vibrio cholerae (156 aa), FASTA scores: opt: 462, E(): 1.8e-22, (47.3% identity in 148 aa overlap); etc.; Probable transcriptional regulatory protein NrdR 3030877 nrdR 887985 nrdR Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NrdR NP_217234.1 3030413 R 83332 CDS NP_217235.1 15609856 887959 complement(3031040..3031537) 1 NC_000962.3 Rv2719c, (MTCY05A6.40c), len: 165 aa. Possible conserved membrane protein, equivalent to Q49846|ML1004|B2235_C3_243 possible conserved membrane protein from Mycobacterium leprae (164 aa), FASTA scores: opt: 486, E(): 4e-21, (55.2% identity in 163 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved membrane protein 3031537 887959 Rv2719c Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_217235.1 3031040 R 83332 CDS NP_217236.2 345462012 888169 3031788..3032498 1 NC_000962.3 Rv2720, (MTCY05A6.41), len: 236 aa. LexA repressor (see citations below), equivalent to Q49848|LEXA_MYCLE|ML1003|B2235_F2_55 LEXA repressor from Mycobacterium leprae (217 aa), FASTA scores: opt: 1255,E(): 7.1e-70, (89.8% identity in 216 aa overlap). Also highly similar to others e.g. O69979|LEXA_STRCO|SC4H2.24c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 1034, E(): 2.6e-56, (70.5% identity in 217 aa overlap); O86847|LEXA_STRCL from Streptomyces clavuligerus (239 aa),FASTA scores: opt: 1021, E(): 1.6e-55, (69.1% identity in 217 aa overlap); Q9KAD3|LEXA_BACHD from Bacillus halodurans (207 aa), FASTA scores: opt: 645, E(): 1.5e-32, (47.9% identity in 213 aa overlap); etc. Belongs to peptidase family S24; also known as the UMUD/LEXA family. Start changed since first submission (+19 aa).; Repressor LexA 3032498 lexA 888169 lexA Mycobacterium tuberculosis H37Rv Repressor LexA NP_217236.2 3031788 D 83332 CDS NP_217237.1 15609858 888291 complement(3032520..3034619) 1 NC_000962.3 Rv2721c, (MTCY05A6.42c, MTCY154.01c), len: 699 aa. Possible conserved transmembrane ala-, gly-rich protein,equivalent to Q49837|ML1002|U2235I possible conserved membrane protein from Mycobacterium leprae (687 aa), FASTA scores: opt: 2703, E(): 6.6e-135, (60.3% identity in 713 aa overlap). Shows some similaity to Q01377|CSP1 PS1 protein precursor (secreted protein) from Corynebacterium glutamicum (Brevibacterium flavum) (657 aa), FASTA scores: opt: 276, E(): 3.8e-07, (29.4% identity in 272 aa overlap); and Q9KIJ0 Rv2721c-like protein from Mycobacterium paratuberculosis (246 aa), FASTA scores: opt: 178, E(): 0.025, (37.5% identity in 120 aa overlap).; Possible conserved transmembrane alanine and glycine rich protein 3034619 888291 Rv2721c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane alanine and glycine rich protein NP_217237.1 3032520 R 83332 CDS NP_217238.1 15609859 888399 3034635..3034883 1 NC_000962.3 Rv2722, (MTCY154.02), len: 82 aa. Conserved hypothetical protein, similar to Q9CCB9|ML1001 hypothetical protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 154, E(): 0.00053, (37.5% identity in 88 aa overlap). Equivalent to AAK47111 from Mycobacterium tuberculosis strain CDC1551 (94 aa) but shorter 12 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3034883 888399 Rv2722 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217238.1 3034635 D 83332 CDS NP_217239.1 15609860 887768 3034909..3036102 1 NC_000962.3 Rv2723, (MTCY154.03), len: 397 aa. Probable conserved integral membrane protein, highly similar to others e.g. Q9Z503|SCC54.23c putative integral membrane export protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 883, E(): 2.4e-48, (46.4% identity in 332 aa overlap); Q9RD18|SCM1.25c putative integral membrane protein from Streptomyces coelicolor (316 aa), FASTA scores: opt: 865, E(): 3.1e-47, (47.55% identity in 324 aa overlap); P96554|Y319_MYXXA integral membrane protein (probable) from Myxococcus xanthus (319 aa), FASTA scores: opt: 626, E(): 3.4e-32, (34.65% identity in 323 aa overlap); P42601|YGJT_ECOLI|B3088 from Escherichia coli strain K12 integral membrane protein (probable) (321 aa),FASTA scores: opt: 541, E(): 7.7e-27, (35.1% identity in 279 aa overlap); etc.; Probable conserved integral membrane protein 3036102 887768 Rv2723 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217239.1 3034909 D 83332 CDS NP_217240.1 15609861 887866 complement(3036131..3037291) 1 NC_000962.3 Rv2724c, (MTCY154.04c), len: 386 aa. Probable fadE20, acyl-CoA dehydrogenase, highly similar to many e.g. Q9X7Y2|SC6A5.36 from Streptomyces coelicolor (382 aa),FASTA scores: opt: 1583, E(): 6.9e-94, (62.7% identity in 378 aa overlap); Q9HVY0|PA4435 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 1468, E(): 1.6e-86, (57.65% identity in 380 aa overlap); Q9ABZ1|CC0079 from Caulobacter crescentus (391 aa), FASTA scores: opt: 1298, E(): 1.2e-75,(51.9% identity in 391 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis proteins e.g. O06164|FADE19|Rv2500c|MTCY07A7.06c acyl-CoA dehydrogenase (394 aa) (34.3% identity in 382 aa overlap). Contains acyl-CoA dehydrogenases signature 2 (PS00073). Belongs to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE20 3037291 fadE20 887866 fadE20 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE20 NP_217240.1 3036131 R 83332 CDS NP_217241.1 15609862 888241 complement(3037427..3038914) 1 NC_000962.3 Rv2725c, (MTCY154.05c), len: 495 aa. Probable hflX (hfl for high frequency of lysogenization), GTP-binding protein ,equivalent to Q9CCC0|ML0997 (alias Q49843|HFLX but longer) possible ATP/GTP-binding protein from Mycobacterium leprae (488 aa), FASTA scores: opt: 2562, E(): 1.1e-133,(84.55% identity in 485 aa overlap). Also highly similar to many e.g. Q9XCC1 from Streptomyces fradiae (425 aa), FASTA scores: opt: 1280, E(): 3.2e-63, (57.7% identity in 423 aa overlap); P73965|HFLX|SLR1521 from Synechocystis sp. strain PCC 6803 (534 aa), FASTA scores: opt: 1028, E(): 2.8e-49,(44.7% identity in 414 aa overlap); P25519|HFLX_ECOLI|B4173 from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 916, E(): 3.4e-43, (40.1% identity in 414 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable GTP-binding protein HflX 3038914 hflX 888241 hflX Mycobacterium tuberculosis H37Rv Probable GTP-binding protein HflX NP_217241.1 3037427 R 83332 CDS NP_217242.1 15609863 888614 complement(3038931..3039800) 1 NC_000962.3 Rv2726c, (MTCY154.06c), len: 289 aa. Probable dapF,diaminopimelate epimerase, equivalent to P46814|DAPF_MYCLE|ML0996|B2235_C3_233 diaminopimelate epimerase from Mycobacterium leprae (296 aa), FASTA scores: opt: 1488, E(): 2.1e-83, (76.05% identity in 292 aa overlap). Also highly similar to O69969|DAPF_STRCO|SC4H2.14 from Streptomyces coelicolor (289 aa), FASTA scores: opt: 439, E(): 1.4e-19, (45.6% identity in 296 aa overlap); and similar to many e.g. O29511|DAPF_ARCFU|AF0747 from Archaeoglobus fulgidus (280 aa), FASTA scores: opt: 310,E(): 9.7e-12, (33.8% identity in 296 aa overlap); Q51564|DAPF_PSEAE|PA5278 from Pseudomonas aeruginosa (276 aa), FASTA scores: opt: 272, E(): 2e-09, (30.15% identity in 292 aa overlap); P08885|DAPF_ECOLI|B3809 from Escherichia coli strain K12 (274 aa), FASTA scores: opt: 266, E(): 4.5e-09, (30.4% identity in 296 aa overlap); etc. Belongs to the diaminopimelate epimerase family.; Probable diaminopimelate epimerase DapF (DAP epimerase) 3039800 dapF 888614 dapF Mycobacterium tuberculosis H37Rv Probable diaminopimelate epimerase DapF (DAP epimerase) NP_217242.1 3038931 R 83332 CDS NP_217243.1 15609864 887242 complement(3039825..3040769) 1 NC_000962.3 Rv2727c, (MTCY154.07c), len: 314 aa. Probable miaA,tRNA delta(2)-isopentenylpyrophosphate transferase,equivalent to P46811|MIAA_MYCLE|ML0995|B2235_C3_232 tRNA delta(2)-isopentenylpyrophosphate transferase from Mycobacterium leprae (311 aa), FASTA scores: opt: 1679,E(): 3.2e-89, (81.85% identity in 314 aa overlap). Also highly similar to many e.g. O69967|MIAA_STRCO|SC4H2.12 from Streptomyces coelicolor (312 aa), FASTA scores: opt: 1006,E(): 1.2e-50, (55.5% identity in 301 aa overlap); O31795|MIAA_BACSU from Bacillus subtilis (314 aa), FASTA scores: opt: 671, E(): 1.9e-31, (38.55% identity in 293 aa overlap);P16384|MIAA_ECOLI|TRPX|B4171 from Escherichia coli strain K12 and Shigella flexneri (316 aa), FASTA scores: opt: 565, E(): 2.3e-25, (35.2% identity in 307 aa overlap);etc. Contains PS00017 ATP/GTP-binding site motif A (P -loop). Belongs to the IPP transferase family.; Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltransferase) (iptase) (IPPT) 3040769 miaA 887242 miaA Mycobacterium tuberculosis H37Rv Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltransferase) (iptase) (IPPT) NP_217243.1 3039825 R 83332 CDS NP_217244.1 15609865 888330 complement(3040766..3041461) 1 NC_000962.3 Rv2728c, (MTCY154.08c), len: 231 aa. Conserved ala-rich protein, equivalent to Q49835|ML0994|B2235_C1_162 hypothetical protein from Mycobacterium leprae (232 aa),FASTA scores: opt: 1037, E(): 1.2e-54, (68.55% identity in 232 aa overlap). Also similar to O69964|SC4H2.09 from Streptomyces coelicolor (237 aa), FASTA scores: opt: 300,E(): 7.7e-11, (32.8% identity in 241 aa overlap); and some similarity with other proteins e.g. Q14234|ELN elastin from Homo sapiens (Human) (757 aa), FASTA scores: opt: 161, E(): 0.03, (30.6% identity in 242 aa overlap); P55488|Y4IE hypothetical 15.4 KDA protein from Rhizobium sp. strain NGR234 (135 aa), FASTA scores: opt: 147, E(): 0.061,(34.95% identity in 123 aa overlap). Shows also some similarity with P71657|Rv1387|MTCY21B4.04 hypothetical protein from Mycobacterium tuberculosis (539 aa), FASTA scores: opt: 159, E(): 0.035, (34.8% identity in 135 aa overlap).; Conserved alanine rich protein 3041461 888330 Rv2728c Mycobacterium tuberculosis H37Rv Conserved alanine rich protein NP_217244.1 3040766 R 83332 CDS NP_217245.1 15609866 888333 complement(3041570..3042475) 1 NC_000962.3 Rv2729c, (MTCY154.09c), len: 301 aa. Probable conserved integral membrane ala-, val-, leu-rich protein,similar to P42459|YLEU_CORGL hypothetical 29.6 KDA protein from Corynebacterium glutamicum (Brevibacterium flavum)(270 aa), FASTA scores: opt: 365, E(): 4.7e-15, (30.75% identity in 221 aa overlap); and to other integral membrane proteins (principally from Streptomyces sp.) e.g. Q9EWZ8|2SCG38.21 from Streptomyces coelicolor (302 aa), FASTA scores: opt: 365, E(): 5.2e-15, (32.0% identity in 278 aa overlap); Q9S267|SCI30A.06 from Streptomyces coelicolor (297 aa),FASTA scores: opt: 356, E(): 1.8e-14, (31.5% identity in 289 aa overlap); AAK81278|CAC3346 from Clostridium acetobutylicum (472 aa), FASTA scores: opt: 154, E(): 0.038, (24.1% identity in 224 aa overlap); etc.; Probable conserved integral membrane alanine valine and leucine rich protein 3042475 888333 Rv2729c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane alanine valine and leucine rich protein NP_217245.1 3041570 R 83332 CDS NP_217246.1 15609867 888323 3042542..3043018 1 NC_000962.3 Rv2730, (MTCY174.10), len: 158 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3043018 888323 Rv2730 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217246.1 3042542 D 83332 CDS NP_217247.1 15609868 888300 3043026..3044378 1 NC_000962.3 Rv2731, (MTCY174.11), len: 450 aa. Conserved ala-,arg-rich protein, highly similar in part to Q49849|B2235_F2_77 hypothetical protein from Mycobacterium leprae (266 aa), FASTA scores: opt: 368, E(): 1e-10, (73.5% identity in 83 aa overlap); and Q9KXN9|SC9C5.35 hypothetical 6.5 KDA protein (fragment) from Streptomyces coelicolor (58 aa), FASTA scores: opt: 214, E(): 0.00065,(51.7% identity in 58 aa overlap). Also similar to Q9L296|SCL2.01 hypothetical 37.4 KDA protein (fragment) from Streptomyces coelicolor (328 aa), FASTA scores: opt: 843, E(): 3.7e-33, (45.95% identity in 296 aa overlap) (but N-terminus shorter); and shows some similarity with other proteins e.g. Q26938 kinetoplast-associated protein (KAP) from Trypanosoma cruzi (1052 aa), FASTA scores: opt: 223,E(): 0.0022, (30.3% identity in 297 aa overlap). Start site chosen by RBS and to avoid overlap, although there are several other possible start sites further upstream.; Conserved alanine and arginine rich protein 3044378 888300 Rv2731 Mycobacterium tuberculosis H37Rv Conserved alanine and arginine rich protein NP_217247.1 3043026 D 83332 CDS NP_217248.1 15609869 888319 complement(3044375..3044989) 1 NC_000962.3 Rv2732c, (MTCY174.12c), len: 204 aa. Probable conserved transmembrane protein, similar to Q49834 hypothetical protein B2235_C1_155 from Mycobacterium leprae (209 aa), FASTA scores: opt: 932, E(): 0, (70.6% identity in 201 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 3044989 888319 Rv2732c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217248.1 3044375 R 83332 CDS NP_217249.1 15609870 888266 complement(3044986..3046524) 1 NC_000962.3 Rv2733c, (MTCY154.13c), len: 512 aa. Conserved hypothetical ala-, arg-rich protein. Similar to other hypothetical proteins from a range of organisms e.g. Y195_MYCLE|Q49842 hypothetical 56.0 kDa protein b2235_c2_195 from Mycobacterium leprae (516 aa), FASTA scores: opt: 2689, E(): 0, (80.4% identity in 509 aa overlap).; Conserved hypothetical alanine, arginine-rich protein 3046524 888266 Rv2733c Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine, arginine-rich protein NP_217249.1 3044986 R 83332 CDS NP_217250.1 15609871 888303 3046821..3047675 1 NC_000962.3 Rv2734, (MTCY154.14), len: 284 aa. Conserved hypothetical protein, highly similar to various proteins e.g. Q984J2|MLR7981 ABC transporter ATP-binding protein from Rhizobium loti (Mesorhizobium loti) (286 aa), FASTA scores: opt: 877, E(): 9e-50, (52.45% identity in 246 aa overlap) (N-terminus longer); Q98DH1|MLL4707 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (249 aa),FASTA scores: opt: 829, E(): 1.1e-46, (50.4% identity in 244 aa overlap); AAK65865|SMA2239 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (259 aa), FASTA scores: opt: 796, E(): 1.5e-44, (50.0% identity in 252 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 3047675 888303 Rv2734 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217250.1 3046821 D 83332 CDS NP_217251.1 15609872 888389 complement(3047560..3048552) 1 NC_000962.3 Rv2735c, (MTCY154.15c), len: 330 aa. Conserved hypothetical protein, showing some similarity with Q98DH2|MLR4706 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (302 aa), FASTA scores: opt: 140, E(): 0.062, (27.0% identity in 200 aa overlap); and Q9PHA1|XF0043 hypothetical protein from Xylella fastidiosa (293 aa), FASTA scores: opt: 120, E(): 1.2, (30.75% identity in 117 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 3048552 888389 Rv2735c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217251.1 3047560 R 83332 CDS NP_217252.1 15609873 888393 complement(3048562..3049086) 1 NC_000962.3 Rv2736c, (MTV002.01c), len: 174 aa. Probable recX,regulatory protein (see citation below), equivalent to P37859|RECX_MYCLE|ML0988|U2235B regulatory protein RECX from Mycobacterium leprae (171 aa), FASTA scores: opt: 848,E(): 2e-46, (77.0% identity in 174 aa overlap); and CAA67596|RECX|P94965|RECX_MYCSM regulatory protein RECX from Mycobacterium smegmatis (188 aa), FASTA scores: opt: 679, E(): 8.8e-36, (66.45% identity in 164 aa overlap). Also similar (or highly similar to) others e.g. O50488|RECX_STRCO|SC4H8.09 from Streptomyces coelicolor (188 aa), FASTA scores: opt: 371, E(): 1.9e-16, (42.7% identity in 164 aa overlap); Q9LCZ3|RECX from Xanthomonas campestris pv. citri (162 aa), FASTA scores: opt: 189, E(): 4.4e-05, (32.45% identity in 151 aa overlap); P37860|RECX_PSEAE|PA3616 from Pseudomonas aeruginosa (153 aa), FASTA scores: opt: 159, E(): 0.0032, (30.65% identity in 137 aa overlap); etc. Belongs to the RecX family.; Regulatory protein RecX 3049086 recX 888393 recX Mycobacterium tuberculosis H37Rv Regulatory protein RecX NP_217252.1 3048562 R 83332 CDS NP_217253.1 15609874 888371 complement(3049052..3051424) 1 NC_000962.3 Rv2737c, (MTV002.02c), len: 790 aa. RecA,recombinase a (see citations below), equivalent to Q59560|RECA_MYCSM RECA protein from Mycobacterium smegmatis (349 aa), FASTA scores: opt: 1495, E(): 1.9e-79, (93.15% identity in 249 aa overlap); and P35901|RECA_MYCLE|ML0987 RECA protein from Mycobacterium leprae (711 aa), FASTA scores: opt: 1217, E(): 4.5e-63, (46.7% identity in 814 aa overlap). Also highly similar to many e.g. Q9REV6|RECA_AMYMD from Amycolatopsis mediterranei (Nocardia mediterranei) (348 aa), FASTA scores: opt: 1450, E(): 7.6e-77, (89.25% identity in 251 aa overlap); P42442|RECA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (376 aa), FASTA scores: opt: 1355,E(): 2.6e-71, (76.55% identity in 273 aa overlap); P41054|RECA_STRAM from Streptomyces ambofaciens (372 aa),FASTA scores: opt: 1347, E(): 7.6e-71, (82.1% identity in 246 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00321 recA signature, and PS00881 Protein splicing signature. Belongs to the RecA family. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the homing endonuclease family in the intein section.; RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)] 3051424 recA 888371 recA Mycobacterium tuberculosis H37Rv RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)] NP_217253.1 3049052 R 83332 CDS YP_177676.1 57117017 3205037 3051619..3051792 1 NC_000962.3 Rv2737A, len: 57 aa. Conserved hypothetical cys-rich protein (possibly gene fragment), similar to central part of AJ243803_1|glgA from Streptomyces coelicolor glgA (181 aa), FASTA scores: opt: 210, E(): 6.1e-09, (59.25% identity in 54 aa overlap).; Conserved hypothetical cysteine rich protein (fragment) 3051792 3205037 Rv2737A Mycobacterium tuberculosis H37Rv Conserved hypothetical cysteine rich protein (fragment) YP_177676.1 3051619 D 83332 CDS NP_217254.1 15609875 888368 complement(3051806..3052012) 1 NC_000962.3 Rv2738c, (MTV002.03c), len: 68 aa. Conserved hypothetical protein, equivalent to Q9CCC1|ML0986 hypothetical protein from Mycobacterium leprae (67 aa),FASTA scores: opt: 397, E(): 3.7e-22, (83.6% identity in 67 aa overlap). Also highly similar to O50484|SC4H8.05 hypothetical 7.5 KDA protein from Streptomyces coelicolor (64 aa), FASTA scores: opt: 185, E(): 5.9e-07, (39.7% identity in 63 aa overlap). Second part of the protein is highly similar to C-terminus of upstream ORF O33285|Rv2742c|MTV002.07c conserved hypothetical protein from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 200, E(): 1.7e-07, (78.4% identity in 37 aa overlap).; hypothetical protein 3052012 888368 Rv2738c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217254.1 3051806 R 83332 CDS NP_217255.1 15609876 888363 complement(3052023..3053189) 1 NC_000962.3 Rv2739c, (MTV002.04c), len: 388 aa. Possible ala-rich transferase, equivalent to Q49841|ML0985|MLCB33.02c|U2235C possible glycosyltransferase from Mycobacterium leprae (392 aa),FASTA scores: opt: 2112, E(): 5.1e-114, (80.95% identity in 388 aa overlap). Shows some similarity with other transferases e.g. Q9S1V2|SCJ4.21 putative glycosyl transferase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 290, E(): 2e-09, (27.75% identity in 382 aa overlap); Q9RYI3|DRA0329 putative glycosyltransferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 267,E(): 4.3e-08, (29.05% identity in 396 aa overlap); P96560|GTFC glycosyltransferase from Amycolatopsis orientalis (409 aa), FASTA scores: opt: 253, E(): 2.7e-07,(27.75% identity in 418 aa overlap); etc. Equivalent to AAK47130 from Mycobacterium tuberculosis strain CDC1551 (420 aa) but shorter 32 aa.; Possible alanine rich transferase 3053189 888363 Rv2739c Mycobacterium tuberculosis H37Rv Possible alanine rich transferase NP_217255.1 3052023 R 83332 CDS NP_217256.1 15609877 888365 3053233..3053682 1 NC_000962.3 Rv2740, (MTV002.05), len: 149 aa. EphG, Epoxide hydrolase, proven biochemically (see Unge et al. 2005),similar to limonene-1,2-epoxide hydrolase capable of hydrolyzing long or bulky lipophilic epoxides. Equivalent,but shorter 17 aa, to Q9CCC2|ML0984 (alias Q49850 but longer) hypothetical protein from Mycobacterium leprae (164 aa), FASTA scores: opt: 481, E(): 9.7e-26, (52.0% identity in 150 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Epoxide hydrolase 3053682 ephG 888365 ephG Mycobacterium tuberculosis H37Rv Epoxide hydrolase NP_217256.1 3053233 D 83332 CDS YP_177902.1 57117018 888339 3053914..3055491 1 NC_000962.3 Rv2741, (MTV002.06), len: 525 aa. PE_PGRS47, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Q10637|YD25_MYCTU|Rv1325c|MT1367|MTCY130.10c hypothetical PE-PGRS family protein (603 aa), FASTA scores: opt: 1936, E(): 1.1e-71, (56.95% identity in 611 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; PE-PGRS family protein PE_PGRS47 3055491 PE_PGRS47 888339 PE_PGRS47 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS47 YP_177902.1 3053914 D 83332 CDS NP_217258.1 15609879 888332 complement(3055515..3056348) 1 NC_000962.3 Rv2742c, (MTV002.07c), len: 277 aa (questionable ORF). Conserved hypothetical arg-rich protein. Extreme N-terminus is highly similar to the N-teminus of Q9CCC1ML0986 hypothetical protein from Mycobacterium leprae (67 aa), FASTA scores: opt: 183, E(): 0.00052, (71.05% identity in 38 aa overlap); and to the downstream ORF O33281|Rv2738c|MTV002.03c conserved hypothetical protein from Mycobacterium tuberculosis (68 aa), FASTA scores: opt: 200, E(): 5.5e-05, (78.4% identity in 37 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Conserved hypothetical arginine rich protein 3056348 888332 Rv2742c Mycobacterium tuberculosis H37Rv Conserved hypothetical arginine rich protein NP_217258.1 3055515 R 83332 CDS NP_217259.1 15609880 887779 complement(3056420..3057232) 1 NC_000962.3 Rv2743c, (MTV002.08c), len: 270 aa. Possible conserved transmembrane ala-rich protein, equivalent to Q49833|MLCB33.04c|B2235_C1_148 unknown protein from Mycobacterium leprae (123 aa), FASTA scores: opt: 639, E(): 3.3e-31, (74.8% identity in 123 aa overlap).; Possible conserved transmembrane alanine rich protein 3057232 887779 Rv2743c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane alanine rich protein NP_217259.1 3056420 R 83332 CDS YP_177903.1 57117019 888304 complement(3057251..3058063) 1 NC_000962.3 Rv2744c, (MTV002.09c), len: 270 aa. 35kd_ag,conserved ala-rich protein 35-kd antigen (see O'Connor et al., 1990). N-terminal part is equivalent to Q49840|MLCB33.06c|B2235_C2_187 hypothetical protein from Mycobacterium leprae (167 aa), FASTA scores: opt: 789, E(): 3.4e-35, (85.05% identity in 147 aa overlap); and C-terminal part equivalent to Q49845|MLCB33.05c|B2235_C3_214 hypothetical protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 465, E(): 3.6e-18, (65.8% identity in 114 aa overlap); note that these two proteins from Mycobacterium leprae are adjacent. Shows some similarity with Q55707||Y617_SYNY3|SLL0617 hypothetical 28.9 KDA protein from Synechocystis sp. strain PCC 6803 (267 aa), FASTA scores: opt: 155, E(): 0.19,(23.4% identity in 252 aa overlap); and C-terminus of Q9L4N1|EMM M protein from Streptococcus equisimilis (592 aa), FASTA scores: opt: 165, E(): 0.11, (23.45% identity in 260 aa overlap). C-terminus also similar to AAK45945|MT1676 conserved hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (85 aa), FASTA scores: opt: 159, E(): 0.047, (50.9% identity in 55 aa overlap). Predicted possible vaccine candidate (See Zvi et al.,2008).; Conserved 35 kDa alanine rich protein 3058063 35kd_ag 888304 35kd_ag Mycobacterium tuberculosis H37Rv Conserved 35 kDa alanine rich protein YP_177903.1 3057251 R 83332 CDS NP_217261.1 15609882 888315 complement(3058193..3058531) 1 NC_000962.3 Rv2745c, (MTV002.10c), len: 112 aa. ClgR,transcriptional regulatory protein, controls protease systems and chaperones.; Transcriptional regulatory protein ClgR 3058531 clgR 888315 clgR Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein ClgR NP_217261.1 3058193 R 83332 CDS NP_217262.1 15609883 888375 complement(3058602..3059231) 1 NC_000962.3 Rv2746c, (MTV002.11c), len: 209 aa. Probable pgsA3,PGP synthase (see citation below), transmembrane protein,equivalent, but longer 19 aa, to Q49839|O08087|PGSA|ML0979 PGSA from Mycobacterium leprae (193 aa), FASTA scores: opt: 925, E(): 3.7e-53, (77.15% identity in 188 aa overlap). Also highly similar to O86813|PGSA phosphatidylglycerophosphate synthase from Streptomyces coelicolor (263 aa), FASTA scores: opt: 692, E(): 6.6e-38,(57.85% identity in 185 aa overlap) (has its N-terminus longer); and similar to others (generally with N-terminus shorter) e.g. Q99XI0|PGSA|SPY2196 phosphatidylglycerophosphate synthase from Streptococcus pyogenes (180 aa), FASTA scores: opt: 368, E(): 5.4e-17,(39.9% identity in 168 aa overlap); Q9ZE96|PGSA_RICPR|PGSA|RP049 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Rickettsia prowazekii (181 aa), FASTA scores: opt: 343, E(): 2.3e-15, (40.1% identity in 172 aa overlap); P06978|PGSA_ECOLI|PGSA|B1912|Z3000|ECS2650 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Escherichia coli strains K12 and O157:H7 (181 aa), FASTA scores: opt: 322, E(): 5.3e-14,(34.45% identity in 180 aa overlap); etc. Also some similarity to PGSA2|Rv1822|MTCY1A11.21c probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Mycobacterium tuberculosis (209 aa), FASTA score: (27.1% identity in 166 aa overlap). Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family.; Probable PGP synthase PgsA3 (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase) (phosphatidylglycerophosphate synthase) 3059231 pgsA3 888375 pgsA3 Mycobacterium tuberculosis H37Rv Probable PGP synthase PgsA3 (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase) (phosphatidylglycerophosphate synthase) NP_217262.1 3058602 R 83332 CDS NP_217263.1 15609884 888407 3059262..3059786 1 NC_000962.3 Rv2747, (MTV002.12), len: 174 aa. Probable argA,alpha-N-acetylglutamate synthase (See Errey et al., 2005). Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to O05559|ML0978|MLCB33.08 putative acetyltransferase from Mycobacterium leprae (180 aa), FASTA scores: opt: 997, E(): 1.2e-57, (86.8% identity in 174 aa overlap). Also similar to various transferases e.g. Q9X8N2|SCE94.27c putative acetyltransferase from Streptomyces coelicolor (169 aa),FASTA scores: opt: 656, E(): 1.3e-35, (60.35% identity in 164 aa overlap); C-terminus of Q9K3D6|ARGH(A) argininosuccinase and N-acetylglutamate synthase from Moritella sp. 2693 (629 aa), FASTA scores: opt: 243, E(): 2e-08, (31.95% identity in 144 aa overlap); C-terminus of Q9JW21|ARGA or NMA0580 putative acetylglutamate synthase from Neisseria meningitidis serogroup a (436 aa), FASTA scores: opt: 201, E(): 7.8e-06, (32.75% identity in 119 aa overlap); etc. Also similar to hypothetical proteins e.g. O67372|AQ_1359 hypothetical 21.1 KDA protein from Aquifex aeolicus (181 aa), FASTA scores: opt: 348, E(): 1.2e-15,(42.35% identity in 137 aa overlap).; Probable L-glutamate alpha-N-acetyltranferase ArgA (alpha-N-acetylglutamate synthase) 3059786 argA 888407 argA Mycobacterium tuberculosis H37Rv Probable L-glutamate alpha-N-acetyltranferase ArgA (alpha-N-acetylglutamate synthase) NP_217263.1 3059262 D 83332 CDS NP_217264.1 15609885 888408 complement(3059855..3062506) 1 NC_000962.3 Rv2748c, (MTV002.13c), len: 883 aa. Possible ftsK,cell division transmembrane protein, equivalent to O05560|ML0977|FTSK|MLCB33.09c cell division protein from Mycobacterium leprae (886 aa), FASTA scores: opt: 3147,E(): 7.9e-175, (78.1% identity in 885 aa overlap). Also similar to other members of the spoIIIE/ftsK family e.g. O86810|SC7C7.05 FTSK homolog from Streptomyces coelicolor (929 aa), FASTA scores: opt: 2256, E(): 3.8e-123, (49.05% identity in 924 aa overlap); Q9CF25|FTSK cell division protein FTSK from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (763 aa), FASTA scores: opt: 1438,E(): 9.1e-76, (37.7% identity in 751 aa overlap); AAK75005|Q97RE4|SP0878 SPOE family protein from Streptococcus pneumoniae (767 aa), FASTA scores: opt: 1405,E(): 7.5e-74, (48.0% identity in 477 aa overlap); P46889|FTSK_ECOLI|B0890 from Escherichia coli strain K12 (1329 aa), FASTA scores: opt: 759, E(): 0, (44.5% identity in 537 aa overlap) (similarity in C-terminal half); etc. Equivalent to AAK47139 from Mycobacterium tuberculosis strain CDC1551 (968 aa) but shorter 85 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the FTSK/SPOIIIE family.; Possible cell division transmembrane protein FtsK 3062506 ftsK 888408 ftsK Mycobacterium tuberculosis H37Rv Possible cell division transmembrane protein FtsK NP_217264.1 3059855 R 83332 CDS NP_217265.1 15609886 888390 3062505..3062819 1 NC_000962.3 Rv2749, (MTV002.14), len: 104 aa. Conserved protein,showing some similarity with Q9I1R9|PA2198 hypothetical protein from Pseudomonas aeruginosa (114 aa), FASTA scores: opt: 157, E(): 0.00081, (35.0% identity in 100 aa overlap); and O86332|Rv0793|MTV042.03 hypothetical 11.2 KDA protein from Mycobacterium tuberculosis (101 aa), FASTA scores: opt: 143, E(): 0.0062, (26.9% identity in 93 aa overlap).; hypothetical protein 3062819 888390 Rv2749 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217265.1 3062505 D 83332 CDS NP_217266.1 15609887 888384 3062816..3063634 1 NC_000962.3 Rv2750, (MTV002.15), len: 272 aa. Probable dehydrogenase, highly similar to other dehydrogenases/reductases e.g. Q9L5X5|cox cholesterol oxidase from Nocardioides simplex (Arthrobacter simplex) (270 aa), FASTA scores: opt: 836, E(): 1.8e-43, (55.7% identity in 264 aa overlap); Q9RA05|LIMC carveol dehydrogenase from Rhodococcus erythropolis (277 aa), FASTA scores: opt: 792, E(): 8.6e-41, (48.55% identity in 274 aa overlap); Q9F5J1|SIM-NJ1|SIMD2 putative 3-keto-acyl-reductase from Streptomyces antibioticus (273 aa), FASTA scores: opt: 435, E(): 3.7e-19, (35.75% identity in 263 aa overlap); etc. Also highly similar to AAK44941MT0715 oxidoreductase (short-chain dehydrogenase/reductase family) from Mycobacterium tuberculosis strain CDC1551 (275 aa), FASTA scores: opt: 702, E(): 2.4e-35, (44.45% identity in 270 aa overlap); and similar to many other Mycobacterium tuberculosis dehydrogenases.; Probable dehydrogenase 3063634 888384 Rv2750 Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_217266.1 3062816 D 83332 CDS NP_217267.1 15609888 887806 3063638..3064528 1 NC_000962.3 Rv2751, (MTV002.16), len: 296 aa. Conserved protein,similar in part to others e.g. Q98LR1|MLR0915 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (299 aa),FASTA scores: opt: 279, E(): 1.6e-11, (32.85% identity in 210 aa overlap); Q9FBX1|SC8E7.10 conserved hypothetical protein from Streptomyces coelicolor (283 aa), FASTA scores: opt: 232, E(): 2.4e-08, (27.9% identity in 269 aa overlap); Q9FMY9 hypothetical protein (genomic DNA,chromosome 5, P1 clone:MJB21) from Arabidopsis thaliana (Mouse-ear cress) (370 aa), FASTA scores: opt: 205, E(): 2.1e-06, (28.9% identity in 211 aa overlap); etc. Also similar in part to several proteins from Mycobacterium tuberculosis: P72053|Rv3787c|MTCY13D12.21 hypothetical 33.4 KDA protein (308 aa), FASTA scores: opt: 266, E(): 1.3e-10,(29.6% identity in 267 aa overlap); O53795|MBE50c|Rv0731c|MTV041.05c hypothetical 34.9 KDA protein (318 aa), FASTA scores: opt: 266, E(): 1.3e-10,(32.05% identity in 281 aa overlap); O53841|Rv0830|MTV043.22 hypothetical 33.4 KDA protein (301 aa), FASTA scores: opt: 263, E(): 2e-10, (31.3% identity in 262 aa overlap); etc. Belongs to the MTCY13D12.21 / MTCY210.45C / MTCY78.29C family.; hypothetical protein 3064528 887806 Rv2751 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217267.1 3063638 D 83332 CDS NP_217268.1 15609889 887802 complement(3064515..3066191) 1 NC_000962.3 Rv2752c, (MTV002.17c), len: 558 aa. Conserved hypothetical protein, equivalent to Q9CBW5|ML1512 hypothetical protein from Mycobacterium leprae (558 aa),FASTA scores: opt: 3301, E(): 1.2e-195, (89.05% identity in 558 aa overlap). Also highly similar to other hypothetical proteins from a wide range of prokaryotes e.g. CAC19480|P54122|YOR4_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (718 aa), FASTA scores: opt: 2142,E(): 3.5e-124, (57.2% identity in 554 aa overlap) (N-terminus longer); O86842|SC9A10.09 from Streptomyces coelicolor (561 aa), FASTA scores: opt: 2077, E(): 2.9e-120, (55.95% identity in 556 aa overlap); Q9ZI80 from Streptomyces toyocaensis (528 aa), FASTA scores: opt: 1843,E(): 7.3e-106, (52.45% identity in 528 aa overlap) (N-terminus shorter 30 aa); etc.; hypothetical protein 3066191 887802 Rv2752c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217268.1 3064515 R 83332 CDS NP_217269.1 15609890 888289 complement(3066222..3067124) 1 NC_000962.3 Rv2753c, (MT2823, MTV002.18c), len: 300 aa. Probable dapA, dihydrodipicolinate synthase, equivalent to Q9CBW4|DAPA_MYCLE|ML1513 dihydrodipicolinate synthase from Mycobacterium leprae (300 aa), FASTA scores: opt: 1699,E(): 2.2e-98, (86.65% identity in 300 aa overlap). Also highly similar to many e.g. P19808|DAPA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (301 aa), FASTA scores: opt: 1089, E(): 2e-60, (58.7% identity in 288 aa overlap); O86841|DAPA_STRCO|SC9A10.08 from Streptomyces coelicolor (299 aa), FASTA scores: opt: 1044,E(): 1.3e-57, (55.75% identity in 287 aa overlap); P05640|DAPA_ECOLI (292 aa), FASTA scores: opt: 515, E(): 0,(33.8% identity in 287 aa overlap); etc. Contains PS00665 and PS00666 Dihydrodipicolinate synthetase signatures 1 and 2. Belongs to the DHDPS family.; Probable dihydrodipicolinate synthase DapA (DHDPS) (dihydrodipicolinate synthetase) 3067124 dapA 888289 dapA Mycobacterium tuberculosis H37Rv Probable dihydrodipicolinate synthase DapA (DHDPS) (dihydrodipicolinate synthetase) NP_217269.1 3066222 R 83332 CDS NP_217270.1 15609891 887766 complement(3067193..3067945) 1 NC_000962.3 Rv2754c, (MTV002.19c), len: 250 aa. Probable thyX,thymidylate synthase, highly similar to Q9CBW3|YF14_MYCLE|ML1514 thymidylate synthase from Mycobacterium leprae (254 aa), FASTA scores: opt: 1351,E(): 1e-84, (81.5% identity in 254 aa overlap). Also highly similar to several others e.g P40111|THYX_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (250 aa), FASTA scores: opt: 1080, E(): 9.8e-67, (62.85% identity in 245 aa overlap); Q05259|THYX_BPML5 Probable thymidylate synthase from Mycobacteriophage L5 (243 aa),FASTA scores: opt: 610, E(): 3.2e-34, (49.55% identity in 220 aa overlap); etc. Contains Pfam match to entry PF02511 Thymidylate synthase complementing protein. Belongs to the THY1 family.; Probable thymidylate synthase ThyX (ts) (TSase) 3067945 thyX 887766 thyX Mycobacterium tuberculosis H37Rv Probable thymidylate synthase ThyX (ts) (TSase) NP_217270.1 3067193 R 83332 CDS YP_177904.1 57117020 887776 complement(3068189..3068464) 1 NC_000962.3 Rv2755c, (MTV002.20c), len: 91 aa. Possible hsdS.1,fragment of type I restriction/modification system specificity determinant (S protein), similar to the N-terminus of other hsdS proteins e.g. O34140|HSDS from Klebsiella pneumoniae (439 aa), FASTA scores: opt: 303,E(): 2.1e-13, (46.65% identity in 90 aa overlap); P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 278, E(): 1.1e-11, (47.65% identity in 86 aa overlap); and Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 144, E(): 0.015, (31.7% identity in 82 aa overlap). Also some similarity with O33303|Rv2761c|MTV002.26c|HSDS possible type I restriction/modification system specificity determinant from Mycobacterium tuberculosis (364 aa), FASTA scores: opt: 145, E(): 0.012, (29.9% identity in 87 aa overlap). Note that previously known as hsdS'.; Possible type I restriction/modification system specificity determinant (fragment) HsdS.1 (S protein) 3068464 hsdS.1 887776 hsdS.1 Mycobacterium tuberculosis H37Rv Possible type I restriction/modification system specificity determinant (fragment) HsdS.1 (S protein) YP_177904.1 3068189 R 83332 CDS NP_217272.1 15609893 888278 complement(3068461..3070083) 1 NC_000962.3 Rv2756c, (MTV002.21c), len: 540 aa. Possible hsdM,type I restriction/modification system DNA methylase (M protein), highly similar to others e.g. Q9P9X8|XF2742 from Xylella fastidiosa (519 aa), FASTA scores: opt: 1613, E(): 1.9e-96, (52.3% identity in 543 aa overlap); O34139|HSDM from Klebsiella pneumoniae (539 aa), FASTA scores: opt: 1267, E(): 4.4e-74, (45.9% identity in 549 aa overlap); P72418|sty|SBLI|HSDM from Salmonella typhimurium (539 aa),FASTA scores: opt: 1263, E(): 8e-74, (45.7% identity in 549 aa overlap); etc. Possible alternative start site (GTG) overlapping with termination codon of previous ORF 90 bp upstream. Note that the corresponding endonuclease (M protein) does not appear to be present in Mycobacterium tuberculosis.; Possible type I restriction/modification system DNA methylase HsdM (M protein) (DNA methyltransferase) 3070083 hsdM 888278 hsdM Mycobacterium tuberculosis H37Rv Possible type I restriction/modification system DNA methylase HsdM (M protein) (DNA methyltransferase) NP_217272.1 3068461 R 83332 CDS NP_217273.1 15609894 888249 complement(3070170..3070586) 1 NC_000962.3 Rv2757c, (MTV002.22c), len: 138 aa. Possible vapC21,toxin, part of toxin-antitoxin (TA) operon with Rv2758c,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in M. tuberculosis e.g. P96411|Rv0229c| MTCY08D5.24c (226 aa), FASTA scores: opt: 354, E(): 4.6e-18, (45.25% identity in 137 aa overlap) (N-terminus longer 89 aa); P95007|RV2546|MTCY159.10c (137 aa), FASTA scores: opt: 265, E(): 7.5e-12, (38.5% identity in 135 aa overlap); O07228|Rv0301|MTCY63.06 (141 aa), FASTA scores: opt: 259, E(): 2.1e-11, (42.4% identity in 132 aa overlap); etc.; Possible toxin VapC21 3070586 vapC21 888249 vapC21 Mycobacterium tuberculosis H37Rv Possible toxin VapC21 NP_217273.1 3070170 R 83332 CDS NP_217274.1 15609895 888263 complement(3070583..3070849) 1 NC_000962.3 Rv2758c, (MTV002.23c), len: 88 aa. Possible vapB21,antitoxin, part of toxin-antitoxin (TA) operon with Rv2757c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in M. tuberculosis e.g. P95008|Rv2545 (92 aa), FASTA scores: opt: 151, E(): 0.00028, (66.65% identity in 45 aa overlap); Q10771|YF60_MYCTU|RV1560|MT1611|MTCY48.05c (72 aa), FASTA scores: opt: 106, E(): 0.52, (39.15% identity in 46 aa overlap); O06565|Rv1113|MTCY22G8.02 (65 aa), FASTA scores: opt: 97, E(): 2.2, (33.35% identity in 69 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature.; Possible antitoxin VapB21 3070849 vapB21 888263 vapB21 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB21 NP_217274.1 3070583 R 83332 CDS NP_217275.1 15609896 888293 complement(3070875..3071270) 1 NC_000962.3 Rv2759c, (MTV002.24c), len: 131 aa. Possible vapC42,toxin, part of toxin-antitoxin (TA) operon with Rv2760c,contains PIN domain, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. O07769|Y609_MYCTU|Rv0609|MT0638|MTCY19H5.13c (133 aa),FASTA scores: opt: 364, E(): 5.1e-18, (49.6% identity in 131 aa overlap); P96914|Y624_MYCTU|Rv0624|MT0652|MTCY20H10.05 (131 aa),FASTA scores: opt: 324, E(): 2.9e-15, (42.85% identity in 126 aa overlap); and Q10874|YJ82_MYCTU|Rv1982c|MT2034|MTCY39.37 (139 aa), FASTA scores: opt: 271, E(): 1.4e-11, (38.6% identity in 127 aa overlap). Also similar to other hypothetical proteins from other bacteria e.g. CAC45376|SMC00900 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (128 aa), FASTA scores: opt: 286, E(): 1.2e-12,(39.55% identity in 129 aa overlap); Q981I7|MLL9357 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (131 aa), FASTA scores: opt: 257, E(): 1.2e-10,(36.35% identity in 132 aa overlap); Q9AAG1|CC0639 hypothetical protein from Caulobacter crescentus (131 aa),FASTA scores: opt: 217, E(): 6.9e-08, (33.35% identity in 132 aa overlap); etc.; Possible toxin VapC42 Contains PIN domain 3071270 vapC42 888293 vapC42 Mycobacterium tuberculosis H37Rv Possible toxin VapC42 Contains PIN domain NP_217275.1 3070875 R 83332 CDS NP_217276.1 15609897 887705 complement(3071267..3071536) 1 NC_000962.3 Rv2760c, (MTV002.25c), len: 89 aa. Possible vapB42,antitoxin, part of toxin-antitoxin (TA) operon with Rv2759c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O07770|Rv0608|MTCY19H5.14c (81 aa), FASTA scores: opt: 128, E(): 0.057, (37.5% identity in 88 aa overlap); and P96913|Rv0623|MTCY20H10.04 (84 aa), FASTA scores: opt: 99, E(): 5.5, (37.1% identity in 89 aa overlap). Also showing some similarity with CAC45377|SMC00899 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (84 aa), FASTA scores: opt: 116, E(): 0.38, (36.25% identity in 91 aa overlap).; Possible antitoxin VapB42 3071536 vapB42 887705 vapB42 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB42 NP_217276.1 3071267 R 83332 CDS NP_217277.1 15609898 887695 complement(3071546..3072640) 1 NC_000962.3 Rv2761c, (MTV002.26c), len: 364 aa. Possible hsdS,type I restriction/modification system specificity determinant (S protein), similar in part to other hsdS protein (S proteins) e.g. Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 252, E(): 7.4e-09,(24.95% identity in 401 aa overlap); N-terminus of Q9RC12 type I S-subunit from Lactobacillus delbrueckii (subsp. lactis) (389 aa), FASTA scores: opt: 232, E(): 1.4e-07,(28.1% identity in 185 aa overlap); N-terminus of P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 221, E(): 8e-07, (28.45% identity in 130 aa overlap); C-terminus of P17222|PRRB_ECOLI from Escherichia coli strain CTR5X (401 aa), FASTA scores: opt: 197, E(): 2.8e-05, (27.05% identity in 148 aa overlap); etc. Seems to belong to type-I restriction system S methylase family.; Possible type I restriction/modification system specificity determinant HsdS (S protein) 3072640 hsdS 887695 hsdS Mycobacterium tuberculosis H37Rv Possible type I restriction/modification system specificity determinant HsdS (S protein) NP_217277.1 3071546 R 83332 CDS NP_217278.1 15609899 887698 complement(3072637..3073056) 1 NC_000962.3 Rv2762c, (MTV002.27c), len: 139 aa. Conserved hypothetical protein, similar to C-terminus of hypothetical proteins: Q9A380|CC3324 from Caulobacter crescentus (409 aa), FASTA scores: opt: 181, E(): 9.8e-05, (43.55% identity in 101 aa overlap); Q98KQ4|MLR1373 from Rhizobium loti (Mesorhizobium loti) (399 aa), FASTA scores: opt: 174, E(): 0.00028, (46.35% identity in 82 aa overlap); and Q9HZZ9|PA2844 from Pseudomonas aeruginosa (402 aa), FASTA scores: opt: 158, E(): 0.0033, (40.0% identity in 80 aa overlap).; hypothetical protein 3073056 887698 Rv2762c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217278.1 3072637 R 83332 CDS NP_217279.1 15609900 887777 complement(3073130..3073609) 1 NC_000962.3 Rv2763c, (MTV002.28c), len: 159 aa. Probable dfrA (alternate gene names: folA, dhfr), dihydrofolate reductase, equivalent to O30463|FOLA dihydrofolate reductase from Mycobacterium avium (see citation below) (181 aa), FASTA scores: opt: 802, E(): 4.5e-48, (70.2% identity in 161 aa overlap); and Q9CBW1|FOLA|ML1518 dihydrofolate reductase from Mycobacterium leprae (165 aa),FASTA scores: opt: 782, E(): 1e-46, (70.55% identity in 163 aa overlap). Also highly similar to many e.g. Q9K168|DYR_NEIMB|FOLA|NMB0308 from Neisseria meningitidis (serogroup B) (162 aa), FASTA scores: opt: 469, E(): 3.8e-25, (46.65% identity in 163 aa overlap); P12833|DYR3_SALTY|DHFRIII from Salmonella typhimurium (162 aa), FASTA scores: opt: 367, E(): 4e-18, (45.4% identity in 141 aa overlap); Q59408|DYRC_ECOLI|DHFRXIII from Escherichia coli strain RA33.2 (165 aa), FASTA scores: opt: 313, E(): 2.2e-14, (41.9% identity in 136 aa overlap); etc. Contains PS00075 Dihydrofolate reductase signature. Belongs to the dihydrofolate reductase family.; Dihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase) 3073609 dfrA 887777 dfrA Mycobacterium tuberculosis H37Rv Dihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase) NP_217279.1 3073130 R 83332 CDS NP_217280.1 15609901 887728 complement(3073680..3074471) 1 NC_000962.3 Rv2764c, (MTV002.29c), len: 263 aa. Probable thyA,thymidylate synthase, equivalent to Q9CBW0|TYSY_MYCLE|THYA|ML1519 thymidylate synthase from Mycobacterium leprae (266 aa), FASTA scores: opt: 1602,E(): 5.9e-102, (85.5% identity in 262 aa overlap). Also highly similar to many e.g. P00470|TYSY_ECOLI|B2827|Z4144|ECS3684|BAB37107|AAG57938 from Escherichia coli strains K12 and O157:H7 (264 aa),FASTA scores: opt: 1309, E(): 5.9e-82, (66.65% identity in 261 aa overlap); P48464|TYSY_SHIFL|THYA from Shigella flexneri (264 aa), FASTA scores: opt: 1303, E(): 1.5e-81,(65.9% identity in 261 aa overlap); P54081|TYSB_BACAM|THYB|THYBA from Bacillus amyloliquefaciens (264 aa), FASTA scores: opt: 1235, E(): 6.7e-77, (66.65% identity in 261 aa overlap); etc. Contains PS00091 Thymidylate synthase active site. Belongs to the thymidylate synthase family.; Probable thymidylate synthase ThyA (ts) (TSASE) 3074471 thyA 887728 thyA Mycobacterium tuberculosis H37Rv Probable thymidylate synthase ThyA (ts) (TSASE) NP_217280.1 3073680 R 83332 CDS NP_217281.1 15609902 887714 3074636..3075373 1 NC_000962.3 Rv2765, (MTV002.30), len: 245 aa. Probable ala-rich hydrolase, similar to various hydrolases or hypothetical proteins e.g. Q9KYM6|SC9H11.13c putative hydrolase from Streptomyces coelicolor (251 aa), FASTA scores: opt: 630,E(): 1.4e-33, (43.1% identity in 246 aa overlap); Q9A5T9|CC2358 dienelactone hydrolase family protein from Caulobacter crescentus (286 aa), FASTA scores: opt: 592,E(): 4.5e-31, (38.45% identity in 242 aa overlap); Q9FCF1|2SCD46.33 putative hydrolase (dienelactone hydrolase family) from Streptomyces coelicolor (254 aa), FASTA scores: opt: 500, E(): 3.9e-25, (37.7% identity in 252 aa overlap); P73163|DLHH_SYNY3|SLL1298 putative carboxymethylenebutenolidase (dienelactone hydrolase) from Synechocystis sp. (strain PCC 6803) (246 aa), FASTA scores: opt: 276, E(): 1.3e-10, (26.95% identity in 230 aa overlap); etc.; Probable alanine rich hydrolase 3075373 887714 Rv2765 Mycobacterium tuberculosis H37Rv Probable alanine rich hydrolase NP_217281.1 3074636 D 83332 CDS YP_177905.1 57117021 887727 complement(3075588..3076370) 1 NC_000962.3 Rv2766c, (MTV002.31c), len: 260 aa. Probable short-chain dehydrogenase/reductase , similar to others (from bacteria and eukaryota) e.g. Q9K3Y8|2SCG61.27c putative short chain oxidoreductase from Streptomyces coelicolor (253 aa), FASTA scores: opt: 722, E(): 7.4e-39,(44.75% identity in 248 aa overlap); Q93790|F54F3.4 hypothetical SDR protein from Caenorhabditis elegans (260 aa), FASTA scores: opt: 613, E(): 6.9e-32, (41.7% identity in 247 aa overlap); O95162|O95162|scad-SRL peroxisomal short-chain alcohol dehydrogenase from Homo sapiens (Human) (260 aa), FASTA scores: opt: 594, E(): 1.1e-30, (39.6% identity in 250 aa overlap); P51831|FABG_BACSU 3-oxoacyl-[acyl-carrier protein] from Bacillus subtilis (246 aa), FASTA scores: opt: 504, E(): 4e-28, (37.2% identity in 247 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis acyl-carrier proteins e.g. MTCY03C7.07 (38.5% identity in 244 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Note that previously known as fabG5, a 3-oxoacyl-[acyl-carrier-protein].; Probable short-chain type dehydrogenase/reductase 3076370 887727 Rv2766c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase YP_177905.1 3075588 R 83332 CDS NP_217283.1 15609904 887762 complement(3076367..3076720) 1 NC_000962.3 Rv2767c, (MTV002.32c), len: 117 aa (questionable ORF). Possible membrane protein, showing very weak similarity with Q9L2H7|SCC121.09 putative metal transport ABC transporter from Streptomyces coelicolor (256 aa),FASTA scores: opt: 110, E(): 1, (33.05% identity in 112 aa overlap).; Possible membrane protein 3076720 887762 Rv2767c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_217283.1 3076367 R 83332 CDS YP_177906.1 57117022 887765 complement(3076894..3078078) 1 NC_000962.3 Rv2768c, (MTV002.33c), len: 394 aa. PPE43, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. upstream ORF O33312|Rv2770c|MTV002.35c (402 aa), FASTA scores: opt: 1135, E(): 6.1e-51, (62.15% identity in 391 aa overlap); and P96362|Rv1039c|MTCY10G2.10 from M. tuberculosis (391 aa), FASTA scores: opt: 1721,E(): 6.8e-81, (70.35% identity in 398 aa overlap). Equivalent to AAK47157 from Mycobacterium tuberculosis strain CDC1551 (462 aa) but shorter 68 aa.; PPE family protein PPE43 3078078 PPE43 887765 PPE43 Mycobacterium tuberculosis H37Rv PPE family protein PPE43 YP_177906.1 3076894 R 83332 CDS YP_177907.1 57117023 888461 complement(3078158..3078985) 1 NC_000962.3 Rv2769c, (MTV002.34c), len: 275 aa. PE27, Member of the Mycobacterium tuberculosis PE family (see citation below), highly similar to many (notably in N-terminal part) e.g. P96361|Rv1040c|MTCY10G2.09 from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 1111, E(): 5.9e-52, (68.55% identity in 283 aa overlap).; PE family protein PE27 3078985 PE27 888461 PE27 Mycobacterium tuberculosis H37Rv PE family protein PE27 YP_177907.1 3078158 R 83332 CDS YP_177677.1 57117024 888456 complement(3079309..3080457) 1 NC_000962.3 Rv2770c, (MTV002.35c), len: 382 aa. PPE44, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. downstream ORF O33310|Rv2768c|MTV002.33c from M. tuberculosis (394 aa), FASTA scores: opt: 1135, E(): 2.2e-53, (62.15% identity in 391 aa overlap); and P96362|Rv1039c|MTCY10G2.10 from Mycobacterium tuberculosis (391 aa), FASTA scores: opt: 1010, E(): 1e-46, (55.95% identity in 395 aa overlap). Equivalent to AAK47159 from Mycobacterium tuberculosis strain CDC1551 (402 aa) but shorter 20 aa. Start changed since first submission (-20 aa).; PPE family protein PPE44 3080457 PPE44 888456 PPE44 Mycobacterium tuberculosis H37Rv PPE family protein PPE44 YP_177677.1 3079309 R 83332 CDS NP_217287.1 15609908 888434 complement(3080581..3081033) 1 NC_000962.3 Rv2771c, (MTV002.36c), len: 150 aa. Conserved hypothetical protein, equivalent to Q9CBV8|ML1525 hypothetical protein from Mycobacterium leprae (151 aa),FASTA scores: opt: 489, E(): 1.7e-27, (52.7% identity in 148 aa overlap). Also highly similar to Q9RD46|SCF56.21 hypothetical 15.7 KDA protein from Streptomyces coelicolor (151 aa), FASTA scores: opt: 671, E(): 2.2e-40, (67.8% identity in 146 aa overlap).; hypothetical protein 3081033 888434 Rv2771c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217287.1 3080581 R 83332 CDS NP_217288.1 15609909 888449 complement(3081119..3081592) 1 NC_000962.3 Rv2772c, (MTV002.37c), len: 157 aa. Probable conserved transmembrane protein, equivalent to Q9CBV7|ML1526 conserved membrane protein from Mycobacterium leprae (160 aa), FASTA scores: opt: 767, E(): 1.5e-43,(76.6% identity in 154 aa overlap); and similar to P46830|YDAB_MYCBO from Mycobacterium bovis (177 aa), FASTA scores: opt: 337, E(): 3.9e-15, (40.75% identity in 135 aa overlap). Also similar to O86837|SC9A10.04 putative membrane protein from Streptomyces coelicolor (151 aa),FASTA scores: opt: 338, E(): 3e-15, (43.75% identity in 144 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 3081592 888449 Rv2772c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217288.1 3081119 R 83332 CDS NP_217289.1 15609910 888443 complement(3081604..3082341) 1 NC_000962.3 Rv2773c, (MTV002.38c), len: 245 aa. DapB,dihydrodipicolinate reductase (see Pavelka et al., 1997),highly similar to many e.g. P40110|DAPB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (248 aa), FASTA scores: opt: 1030, E(): 1.8e-58, (65.45% identity in 246 aa overlap); O86836|DAPB_STRCO|SC9A10.03 from Streptomyces coelicolor (250 aa), FASTA scores: opt: 997, E(): 2.3e-56, (61.15% identity in 247 aa overlap); P42976|DAPB_BACSU from Bacillus subtilis (267 aa), FASTA scores: opt: 608, E(): 1.7e-31, (45.95% identity in 209 aa overlap); P46829|DAPB_MYCBO from Mycobacterium bovis (see Cirillo et al., 1994) (271 aa), FASTA scores: opt: 505,E(): 6.3e-25, (36.2% identity in 246 aa overlap); etc. Belongs to the dihydrodipicolinate reductase family.; Dihydrodipicolinate reductase DapB (DHPR) 3082341 dapB 888443 dapB Mycobacterium tuberculosis H37Rv Dihydrodipicolinate reductase DapB (DHPR) NP_217289.1 3081604 R 83332 CDS NP_217290.1 15609911 888439 complement(3082352..3082756) 1 NC_000962.3 Rv2774c, (MTV002.39c), len: 134 aa. Hypothetical unknown protein.; Hypothetical protein 3082756 888439 Rv2774c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217290.1 3082352 R 83332 CDS NP_217291.3 448824789 888432 3082909..3083370 1 NC_000962.3 Rv2775, (MTV002.40), len: 153 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Showing weak similarity to other hypothetical proteins e.g. Q9ZBJ7|SC9C7.13c from Streptomyces coelicolor (179 aa),FASTA scores: opt: 167, E(): 0.00024, (29.05% identity in 148 aa overlap). Equivalent to AAK47164 from Mycobacterium tuberculosis strain CDC1551 (185 aa) but shorter 32 aa.; GCN5-related N-acetyltransferase 3083370 888432 Rv2775 Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_217291.3 3082909 D 83332 CDS NP_217292.1 15609913 888453 complement(3083374..3084303) 1 NC_000962.3 Rv2776c, (MTV002.41c), len: 309 aa. Probable oxidoreductase, similar to other oxidoreductases e.g. Q9KZ15|SC10B7.17 putative iron-sulfur oxidoreductase from Streptomyces coelicolor (364 aa), FASTA scores: opt: 846,E(): 1.2e-45, (46.75% identity in 308 aa overlap); O88034|SC5A7.28c iron-sulfur oxidoreductase beta subunit from Streptomyces coelicolor (313 aa), FASTA scores: opt: 745, E(): 2.3e-39, (41.45% identity in 316 aa overlap); P33164|PDR_BURCE|OPHA1 phthalate dioxygenase reductase from Burkholderia cepacia (Pseudomonas cepacia) (321 aa), FASTA scores: opt: 616, E(): 2.9e-31, (33.65% identity in 309 aa overlap); etc. Equivalent to AAK47165 from Mycobacterium tuberculosis strain CDC1551 (363 aa) but shorter 54 aa. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature and PS00063 Aldo/keto reductase family putative active site signature. Seems to belong to the 2FE2S plant-type ferredoxin family in the C-terminal section.; Probable oxidoreductase 3084303 888453 Rv2776c Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_217292.1 3083374 R 83332 CDS NP_217293.1 15609914 887833 complement(3084485..3085555) 1 NC_000962.3 Rv2777c, (MTV002.42c), len: 356 aa. Conserved hypothetical protein, highly similar (but longer in N-terminus) to hypothetical proteins Q9KZ16|SC10B7.16 from Streptomyces coelicolor (296 aa), FASTA scores: opt: 980,E(): 6.8e-57, (51.25% identity in 281 aa overlap); and Q9HYS0|PA3325 from Pseudomonas aeruginosa (295 aa), FASTA scores: opt: 816, E(): 4e-46, (43.75% identity in 288 aa overlap); and similar (but longer in N-terminus) to other hypothetical proteins e.g. Q9I3H1|PA1542 from Pseudomonas aeruginosa (278 aa), FASTA scores: opt: 234, E(): 6.3e-08,(31.8% identity in 258 aa overlap). Equivalent to AAK47166 from Mycobacterium tuberculosis strain CDC1551 (393 aa) but shorter 37 aa.; hypothetical protein 3085555 887833 Rv2777c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217293.1 3084485 R 83332 CDS NP_217294.1 15609915 887751 complement(3085713..3086183) 1 NC_000962.3 Rv2778c, (MTV002.43c), len: 156 aa. Conserved protein, similar to Q9CBF7|ML2031 hypothetical protein from Mycobacterium leprae (151 aa), FASTA scores: opt: 227, E(): 8.5e-09, (35.95% identity in 153 aa overlap). Also similar to AAK46204|MT1931.1 hypothetical 17.8 KDA protein from Mycobacterium tuberculosis strain CDC1551 (158 aa), FASTA scores: opt: 238, E(): 1.5e-09, (35.75% identity in 151 aa overlap); or O07748|Rv1883c|MTCY180.35 hypothetical 17.3 KDA protein from Mycobacterium tuberculosis strain H37Rv (158 aa), FASTA scores: opt: 212, E(): 9.7e-08, (34.45% identity in 151 aa overlap); note that AAK46204|MT1931.1 and O07748|Rv1883c|MTCY180.35 are essentially the same protein except for a small (5 aa) gap.; hypothetical protein 3086183 887751 Rv2778c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217294.1 3085713 R 83332 CDS NP_217295.2 57117025 888479 complement(3086215..3086754) 1 NC_000962.3 Rv2779c, (MTV002.44c), len: 179 aa. Possible transcriptional regulator, from the Lrp/AsnC family,similar (but longer ~30 aa in N-terminus) to others e.g. CAC42842|SCBAC36F5.06 putative AsnC-family transcriptional regulatory protein from Streptomyces coelicolor (163 aa),FASTA scores: opt: 333, E(): 4.4e-16, (39.7% identity in 141 aa overlap); O07920|AZLB_BACSU transcriptional regulator (AsnC family) from Bacillus subtilis; Q9I233|PA2082 probable transcriptional regulator (AsnC family) from Pseudomonas aeruginosa (158 aa), FASTA scores: opt: 322, E(): 2.5e-15, (33.1% identity in 148 aa overlap); etc. Also similar to P96896|Rv3291c|MTCY71.31c from Mycobacterium tuberculosis (33.3% identity in 120 aa overlap). Equivalent to AAK47168 from Mycobacterium tuberculosis strain CDC1551 (181 aa). Seems to belong to the AsnC family of transcriptional regulators. Start changed since first submission (+8 aa).; Possible transcriptional regulatory protein (probably Lrp/AsnC-family) 3086754 888479 Rv2779c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably Lrp/AsnC-family) NP_217295.2 3086215 R 83332 CDS NP_217296.1 15609917 888493 3086820..3087935 1 NC_000962.3 Rv2780, (MT2850, MTV002.45), len: 371 aa. Ald,secreted L-alanine dehydrogenase (40 kd antigen); equivalent to Q9CBV6|ALD|ML1532 L-alanine dehydrogenase from Mycobacterium leprae (371 aa), FASTA scores: opt: 2081, E(): 4e-115, (85.45% identity in 371 aa overlap). Also highly similar to others e.g. Q9S227|SCI51.13c from Streptomyces coelicolor (371 aa), FASTA scores: opt: 1575,E(): 2.3e-85, (66.05% identity in 371 aa overlap); Q9K827|BH3180 from Bacillus halodurans (371 aa), FASTA scores: opt: 1341, E(): 1.4e-71, (56.45% identity in 372 aa overlap); Q9RT70|DR1895 from Deinococcus radiodurans (390 aa), FASTA scores: opt: 1319, E(): 2.8e-70, (54.2% identity in 371 aa overlap); etc. Contains PS00836 and PS00837 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1 and 2. Predicted possible vaccine candidate (See Zvi et al., 2008).; Secreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43) 3087935 ald 888493 ald Mycobacterium tuberculosis H37Rv Secreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43) NP_217296.1 3086820 D 83332 CDS NP_217297.1 15609918 888488 complement(3087950..3088984) 1 NC_000962.3 Rv2781c, (MTV002.46c), len: 344 aa. Possible ala-rich oxidoreductase, similar to various oxidoreductases or hypothetical proteins e.g. Q9RDD8|SCC77.20c putative oxidoreductase from Streptomyces coelicolor (364 aa), FASTA scores: opt: 912, E(): 5.3e-47, (45.55% identity in 336 aa overlap); Q9FDD4|2-NPDL putative 2-nitropropane dioxygenase from Streptomyces ansochromogenes (363 aa), FASTA scores: opt: 869, E(): 1.9e-44, (44.2% identity in 337 aa overlap); O05413|YRPB 2-nitropropane dioxygenase from Bacillus subtilis (347 aa), FASTA scores: opt: 560, E(): 4.9e-26,(33.75% identity in 317 aa overlap); etc.; Possible alanine rich oxidoreductase 3088984 888488 Rv2781c Mycobacterium tuberculosis H37Rv Possible alanine rich oxidoreductase NP_217297.1 3087950 R 83332 CDS NP_217298.1 15609919 888470 complement(3089045..3090361) 1 NC_000962.3 Rv2782c, (MTV002.47c), len: 438 aa. Probable pepR,protease/peptidase, equivalent to O32965|YR82_MYCLE|ML0855|MLCB22.26c hypothetical zinc protease from Mycobacterium leprae (445 aa), FASTA scores: opt: 2346, E(): 4.3e-146, (84.3% identity in 421 aa overlap). Also highly similar to others e.g. O86835|YA12_STRCO|SC9A10.02 from Streptomyces coelicolor (459 aa), FASTA scores: opt: 1394, E(): 1.1e-83, (51.9% identity in 416 aa overlap); Q04805|YMXG_BACSU|YMXG from Bacillus subtilis (409 aa), FASTA scores: opt: 1014, E(): 7.9e-59, (37.55% identity in 410 aa overlap); Q9KA85|BH2405 from Bacillus halodurans (413 aa), FASTA scores: opt: 967,E(): 9.6e-56, (38.6% identity in 417 aa overlap); etc. Contains PS00143 Insulinase family, zinc-binding region signature. Belongs to peptidase family M16, also known as the insulinase family. Cofactor: requires divalent cations for activity. Binds zinc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable zinc protease PepR 3090361 pepR 888470 pepR Mycobacterium tuberculosis H37Rv Probable zinc protease PepR NP_217298.1 3089045 R 83332 CDS NP_217299.1 15609920 888467 complement(3090339..3092597) 1 NC_000962.3 Rv2783c, (MTV002.48c), len: 752 aa. Probable gpsI,polyribonucleotide nucleotidyltransferase, equivalent to Q9CCF8|GPSI|ML0854 (alias O32966) putative polyribonucleotide phosphorylase / guanosine pentaphosphate synthetase from Mycobacterium leprae (773 aa), FASTA scores: opt: 4304, E(): 0, (89.95% identity in 757 aa overlap). Also highly similar to others e.g. O86656|GPSI guanosine pentaphosphate synthetase/ polyribonucleotide nucleotidyltransferase (fragment) from Streptomyces coelicolor (716 aa), FASTA scores: opt: 3393, E(): 5.8e-192, (72.77% identity in 718 aa overlap); Q53597|GPSI guanosine pentaphosphate synthetase from Streptomyces antibioticus (740 aa), FASTA scores: opt: 3314, E(): 2.6e-187, (70.55% identity in 733 aa overlap); P72659|PNP|SLL1043 polyribonucleotide nucleotidyltransferase from Synechocystis sp. strain PCC 6803 (718 aa), FASTA scores: opt: 1244, E(): 1.7e-65,(45.05% identity in 750 aa overlap); etc. Note that S. antibioticus guanosine pentaphosphate synthetase is a multifunctional enzyme that also acts as a polyribonucleotide nucleotidyltransferase. Start site chosen by homology from several alternatives.; Bifunctional protein polyribonucleotide nucleotidyltransferase GpsI: guanosine pentaphosphate synthetase + polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase) (pnpase) 3092597 gpsI 888467 gpsI Mycobacterium tuberculosis H37Rv Bifunctional protein polyribonucleotide nucleotidyltransferase GpsI: guanosine pentaphosphate synthetase + polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase) (pnpase) NP_217299.1 3090339 R 83332 CDS NP_217300.1 15609921 888484 complement(3092951..3093466) 1 NC_000962.3 Rv2784c, (MTV002.49c), len: 171 aa. Probable lppU,lipoprotein, sharing no homology with other proteins. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LppU 3093466 lppU 888484 lppU Mycobacterium tuberculosis H37Rv Probable lipoprotein LppU NP_217300.1 3092951 R 83332 CDS NP_217301.1 15609922 888455 complement(3093479..3093748) 1 NC_000962.3 Rv2785c, (MTV002.50c), len: 89 aa. rpsO, 30s ribosomal protein S15, equivalent to O32967|RS15_MYCLE|RPSO|ML0853|MLCB22.28c 30S ribosomal protein S15 from Mycobacterium leprae (89 aa), FASTA scores: opt: 522, E(): 7.4e-34, (92.15% identity in 89 aa overlap). Also highly similar to many e.g. O86655|RS15_STRCO|RPSO|SC3C3.22 from Streptomyces coelicolor (95 aa), FASTA scores: opt: 408, E(): 6.7e-25,(62.9% identity in 89 aa overlap); P05766|RS15_BACST|RPSO from Bacillus stearothermophilus (88 aa), FASTA scores: opt: 385, E(): 4e-23, (62.5% identity in 88 aa overlap); P21473|RS15_BACSU|RPSO from Bacillus subtilis (88 aa),FASTA scores: opt: 351, E(): 1.9e-20, (57.95% identity in 88 aa overlap); P02371|RS15_ECOLI|RPSO|sec|B3165 from Escherichia coli strain K12 (88 aa), FASTA scores: opt: 295, E(): 4.5e-22, (52.3% identity in 88 aa overlap); etc. Contains PS00362 Ribosomal protein S15 signature. Belongs to the S15P family of ribosomal proteins.; 30S ribosomal protein S15 RpsO 3093748 rpsO 888455 rpsO Mycobacterium tuberculosis H37Rv 30S ribosomal protein S15 RpsO NP_217301.1 3093479 R 83332 CDS NP_217302.1 15609923 888468 complement(3093905..3094900) 1 NC_000962.3 Rv2786c, (MTV002.51c), len: 331 aa. Probable ribF,FAD synthetase/riboflavin biosynthesis protein,bifunctional enzyme, equivalent to O32968|RIBF|ML0852 riboflavin kinase from Mycobacterium leprae (331 aa), FASTA scores: opt: 1923, E(): 2.3e-115, (87.45% identity in 327 aa overlap). Also highly similar to many e.g. Q59263|RIBF_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (338 aa), FASTA scores: opt: 899, E(): 5.7e-50, (45.8% identity in 321 aa overlap); Q9Z530|SC9F2.05c from Streptomyces coelicolor (318 aa),FASTA scores: opt: 862, E(): 1.3e-47, (52.45% identity in 324 aa overlap); P08391|RIBF_ECOLI|B0025|Z0029\ECS0028 from Escherichia coli strains K12 and O157:H7 (313 aa), FASTA scores: opt: 517, E(): 1.3e-25, (36.05% identity in 305 aa overlap); etc.; Probable bifunctional FAD synthetase/riboflavin biosynthesis protein RibF: riboflavin kinase (flavokinase) + FMN adenylyltransferase (FAD pyrophosphorylase) (FAD synthetase)(FAD diphosphorylase) (flavin adenine dinucleotide synthetase) 3094900 ribF 888468 ribF Mycobacterium tuberculosis H37Rv Probable bifunctional FAD synthetase/riboflavin biosynthesis protein RibF: riboflavin kinase (flavokinase) + FMN adenylyltransferase (FAD pyrophosphorylase) (FAD synthetase)(FAD diphosphorylase) (flavin adenine dinucleotide synthetase) NP_217302.1 3093905 R 83332 CDS NP_217303.1 15609924 888288 3095111..3096874 1 NC_000962.3 Rv2787, (MTV002.52), len: 587 aa. Conserved hypothetical ala-rich protein, equivalent to Q9CCI1|ML0798 hypothetical protein from Mycobacterium leprae (592 aa),FASTA scores: opt: 2994, E(): 6.9e-179, (76.5% identity in 587 aa overlap); and similar in part to other proteins from Mycobacterium leprae e.g. O33082|MLCB628.11 hypothetical 52.0 KDA protein (478 aa), FASTA scores: opt: 481, E(): 2.3e-22, (30.95% identity in 294 aa overlap). Also similar in part to O86637|SC3C3.03c hypothetical 112.1 KDA protein from Streptomyces coelicolor (1083 aa), FASTA scores: opt: 488, E(): 1.5e-22, (28.95% identity in 297 aa overlap). And similar to other hypothetical proteins from Mycobacterium tuberculosis e.g. O06396|Rv0530|MTCY25D10.09 (405 aa),FASTA scores: opt: 625, E(): 2.2e-31, (34.05% identity in 320 aa overlap); O69740|Rv3876|MTV027.11 (666 aa), FASTA scores: opt: 453, E(): 1.6e-20, (29.2% identity in 370 aa overlap); P96217|Rv3860|MTCY01A6.08c (390 aa), FASTA scores: opt: 443, E(): 4.7e-20, (29.95% identity in 354 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Conserved hypothetical alanine rich protein 3096874 888288 Rv2787 Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine rich protein NP_217303.1 3095111 D 83332 CDS NP_217304.1 15609925 888253 3096959..3097645 1 NC_000962.3 Rv2788, (MTV002.53), len: 228 aa. Probable sirR,transcriptional repressor, highly similar to others e.g. Q9RRF3|DR2539 putative iron dependent repressor from Deinococcus radiodurans (232 aa), FASTA scores: opt: 518,E(): 4.5e-26, (41.2% identity in 221 aa overlap); Q9HRU8|SIRR|VNG0536G from Halobacterium sp. strain NRC-1 (233 aa), FASTA scores: opt: 516, E(): 6.1e-26, (40.45% identity in 220 aa overlap); Q9KIJ2|SLOR regulator SLOR from Streptococcus mutans (217 aa), FASTA scores: opt: 418,E(): 1.2e-19, (36.15% identity in 213 aa overlap); etc. Also some similarity to Q50495|IDER_MYCTU|MTCY05A6.32|IDER|DTXR|Rv2711|MT2784|MTCY 05A6.32 iron-dependent repressor from Mycobacterium tuberculosis (230 aa), FASTA scores: opt: 266, E(): 7.1e-10, (27.6% identity in 221 aa overlap). Contains helix-turn-helix motif at aa 32-53 (Score 1327, +3.71 SD). Could belong to the Crp/Fnr family of transcriptional regulators.; Probable transcriptional repressor SirR 3097645 sirR 888253 sirR Mycobacterium tuberculosis H37Rv Probable transcriptional repressor SirR NP_217304.1 3096959 D 83332 CDS NP_217305.1 15609926 888258 complement(3097706..3098938) 1 NC_000962.3 Rv2789c, (MTV002.54c), len: 410 aa. Probable fadE21,acyl-CoA dehydrogenase, similar to many e.g. P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa),FASTA scores: opt: 689, E(): 9.3e-37, (35.75% identity in 400 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 679, E(): 4.1e-36,(37.3% identity in 405 aa overlap); Q06319|ACDS_MEGEL from Megasphaera elsdenii (383 aa), FASTA scores: opt: 650, E(): 3e-34, (37.7% identity in 334 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 1 (PS00072). Belongs to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE21 3098938 fadE21 888258 fadE21 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE21 NP_217305.1 3097706 R 83332 CDS NP_217306.3 448824790 888585 complement(3098964..3100169) 1 NC_000962.3 Rv2790c, (MTV002.55c), len: 401 aa. Probable ltp1,lipid-transfer protein, highly similar to many eukaryotic sterol-carrier proteins/lipid-transfer protein precursors (see Ossendorp & Wirtz 1993) e.g. O62742|SCP2 sterol carrier protein X from Oryctolagus cuniculus (Rabbit) (547 aa), FASTA scores: opt: 1710, E(): 6e-102, (63.7% identity in 394 aa overlap); Q9QW19 3-oxoacyl-CoA thiolase homolog (fragment) from Rattus sp. (405 aa), FASTA scores: opt: 1696, E(): 3.8e-101, (63.2% identity in 394 aa overlap); P11915|NLTP_RAT|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Rattus norvegicus (Rat) (547 aa),FASTA scores: opt: 1696, E(): 4.8e-101, (63.2% identity in 394 aa overlap); P32020|NLTP_MOUSE|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Mus musculus (Mouse) (547 aa), FASTA scores: opt: 1681, E(): 4.3e-100, (62.7% identity in 394 aa overlap); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature and PS00737 Thiolases signature 2. Also similar to other M. tuberculosis proteins e.g. O06144|Rv1627c|MTCY01B2.19c (402 aa) (35.8% identity in 413 aa overlap).; Probable lipid-transfer protein Ltp1 3100169 ltp1 888585 ltp1 Mycobacterium tuberculosis H37Rv Probable lipid-transfer protein Ltp1 NP_217306.3 3098964 R 83332 CDS NP_217307.1 15609928 888281 complement(3100202..3101581) 1 NC_000962.3 Rv2791c, (MTV002.56c), len: 459 aa. Probable IS1602 transposase for IS1602 element, similar to many e.g. P95117|Rv2978c|MTCY349.09 from Mycobacterium tuberculosis (459 aa), FASTA scores: opt: 2718, E(): 6.3e-165, (86.05% identity in 459 aa overlap).; Probable transposase 3101581 888281 Rv2791c Mycobacterium tuberculosis H37Rv Probable transposase NP_217307.1 3100202 R 83332 CDS NP_217308.1 15609929 888274 complement(3101581..3102162) 1 NC_000962.3 Rv2792c, (MTV002.57c), len: 193 aa. Possible IS1602 resolvase, highly similar to many from Mycobacterium tuberculosis e.g. O07773|Rv0605|MTCY19H5.17c possible resolvase (202 aa), FASTA scores: opt: 1040, E(): 1.9e-62,(85.05% identity in 194 aa overlap). Contains PS00397 Site-specific recombinases active site and possible helix-turn-helix motif at aa 1-2 (Score 1687, +4.93 SD).; Possible resolvase 3102162 888274 Rv2792c Mycobacterium tuberculosis H37Rv Possible resolvase NP_217308.1 3101581 R 83332 CDS NP_217309.1 15609930 888587 complement(3102364..3103260) 1 NC_000962.3 Rv2793c, (MTV002.58c), len: 298 aa. Probable truB,tRNA pseudouridine synthase, equivalent to Q9Z5I4|TRUB_MYCLE|ML1546 or MLCB596.24 tRNA pseudouridine synthase B from Mycobacterium leprae (320 aa), FASTA scores: opt: 1403, E(): 2.9e-83, (74.05% identity in 293 aa overlap). Also highly similar to many e.g. Q9Z528|TRUB_STRCO|SC9F2.07c from Streptomyces coelicolor (301 aa), FASTA scores: opt: 870, E(): 7.6e-49, (50.7% identity in 296 aa overlap); P09171|TRUB_ECOLI|P35|B3166|Z4527|ECS4047 from Escherichia coli strains K12 and O157:H7 (314 aa), FASTA scores: opt: 574, E(): 1e-29, (42.5% identity in 214 aa overlap); Q9PGR1|TRUB_XYLFA|XF0237 from Xylella fastidiosa (302 aa),FASTA scores: opt: 569, E(): 2.1e-29, (41.05% identity in 285 aa overlap); etc. Belongs to the TruB family of pseudouridine synthases.; Probable tRNA pseudouridine synthase B TruB (tRNA pseudouridine 55 synthase) (PSI55 synthase) (pseudouridylate synthase) (uracil hydrolyase) 3103260 truB 888587 truB Mycobacterium tuberculosis H37Rv Probable tRNA pseudouridine synthase B TruB (tRNA pseudouridine 55 synthase) (PSI55 synthase) (pseudouridylate synthase) (uracil hydrolyase) NP_217309.1 3102364 R 83332 CDS NP_217310.1 15609931 888226 complement(3103257..3103940) 1 NC_000962.3 Rv2794c, (MTV002.59c), len: 227 aa. PptT,phosphopantetheinyl transferase, equivalent to Q9Z5I5|ML1547|MLCB596.23 putative iron-chelating complex subunit from Mycobacterium leprae (227 aa), FASTA scores: opt: 1248, E(): 9.1e-77, (79.75% identity in 227 aa overlap). Also highly similar to various proteins e.g. Q9F0Q6|PPTA phosphopantetheinyl transferase from Streptomyces verticillus (246 aa), FASTA scores: opt: 692,E(): 2.8e-39, (46.65% identity in 225 aa overlap); O88029|SC5A7.23 hypothetical 24.5 KDA protein from Streptomyces coelicolor (226 aa), FASTA scores: opt: 679,E(): 2e-38, (46.9% identity in 226 aa overlap); O24813 DNA for L-proline 3-hydroxylase from Streptomyces sp. (208 aa),FASTA scores: opt: 631, E(): 3.2e-35, (48.1% identity in 208 aa overlap); etc.; Phosphopantetheinyl transferase PptT (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) 3103940 pptT 888226 pptT Mycobacterium tuberculosis H37Rv Phosphopantetheinyl transferase PptT (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) NP_217310.1 3103257 R 83332 CDS NP_217311.1 15609932 888492 complement(3103937..3104911) 1 NC_000962.3 Rv2795c, (MTV002.60c), len: 324 aa. Conserved hypothetical protein, equivalent to Q9Z5I6|ML1548|MLCB596.22 hypothetical 37.5 KDA protein from Mycobacterium leprae (321 aa), FASTA scores: opt: 2018,E(): 6.3e-128, (87.4% identity in 318 aa overlap). Also highly similar to O88028|SC5A7.22 hypothetical 33.5 KDA protein from Streptomyces coelicolor (295 aa), FASTA scores: opt: 1202, E(): 3.4e-73, (57.2% identity in 285 aa overlap); and Q9AMH7|SIMX4 SIMX4 protein from Streptomyces antibioticus (293 aa), FASTA scores: opt: 1045, E(): 1.2e-62, (51.4% identity in 286 aa overlap). C-terminus highly similar to Q9F0Q7 hypothetical 9.6 KDA protein (fragment) from Streptomyces verticillus (81 aa), FASTA scores: opt: 395, E(): 1.8e-19, (68.35% identity in 79 aa overlap). Also similar to other proteins e.g. Q9FWV7 hypothetical 45.3 KDA protein from Oryza sativa (Rice) (402 aa), FASTA scores: opt: 294, E(): 3.6e-12, (26.45% identity in 340 aa overlap).; hypothetical protein 3104911 888492 Rv2795c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217311.1 3103937 R 83332 CDS NP_217312.1 15609933 888915 complement(3105056..3105619) 1 NC_000962.3 Rv2796c, (MTV002.61c, MTCY16B7.47), len: 187 aa. Probable lppV, conserved lipoprotein, similar to others from Mycobacterium tuberculosis e.g. P95009|LPPB|Rv2544|MTCY159.12c probable conserved lipoprotein (220 aa), FASTA scores: opt: 168, E(): 0.00066,(22.45% identity in 196 aa overlap); and P95010|LPPA|RV2543|MTCY159.13c probable conserved lipoprotein (219 aa), FASTA scores: opt: 165, E(): 0.001,(23.1% identity in 199 aa overlap).; Probable conserved lipoprotein LppV 3105619 lppV 888915 lppV Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppV NP_217312.1 3105056 R 83332 CDS NP_217313.1 15609934 887713 complement(3105619..3107307) 1 NC_000962.3 Rv2797c, (MTCY16B7.46), len: 562 aa. Conserved hypothetical ala-rich protein. C-terminus highly similar to several mycobacterial proteins e.g. AAK46927|MT2616 hypothetical 28.0 KDA protein from Mycobacterium tuberculosis strain CDC1551 (265 aa), FASTA scores: opt: 535, E(): 4.6e-22, (42.95% identity in 263 aa overlap); P95011|Rv2542|MTCY159.14c hypothetical 42.4 KDA protein from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 537, E(): 5e-22, (40.75% identity in 292 aa overlap) (similarity in the second half of protein); P71547|Y963_MYCTU|Rv0963c|MT0992|MTCY10D7.11 hypothetical 28.1 KDA protein (266 aa), FASTA scores: opt: 314, E(): 5.7e-10, (39.0% identity in 254 aa overlap); etc. Contains PS00120 Lipases, serine active site.; hypothetical protein 3107307 887713 Rv2797c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217313.1 3105619 R 83332 CDS NP_217314.1 15609935 888431 complement(3107311..3107637) 1 NC_000962.3 Rv2798c, (MTCY16B7.45), len: 108 aa. Conserved hypothetical ala-rich protein, similar to P71545|Y965_MYCTU|Rv0965c|MT0993|MTCY10D7.09 hypothetical 14.5 KDA protein from Mycobacterium tuberculosis (139 aa),FASTA scores: opt: 198, E(): 8e-07, (38.9% identity in 90 aa overlap).; hypothetical protein 3107637 888431 Rv2798c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217314.1 3107311 R 83332 CDS NP_217315.1 15609936 887759 3107768..3108397 1 NC_000962.3 Rv2799, (MTCY16B7.44c), len: 209 aa. Probable membrane protein. Predicted to be an outer membrane protein (See Song et al., 2008).; Probable membrane protein 3108397 887759 Rv2799 Mycobacterium tuberculosis H37Rv Probable membrane protein NP_217315.1 3107768 D 83332 CDS NP_217316.1 15609937 888932 3108416..3110065 1 NC_000962.3 Rv2800, (MTCY16B7.43c), len: 549 aa. Possible hydrolase, an esterase or an acylase. Similar, but longer in N-terminus, to esterases or acylases e.g. Q9L9D7|COCE cocaine esterase from Rhodococcus sp. MB1 'Bresler 1999' (574 aa), FASTA scores: opt: 510, E(): 3.1e-23, (33.6% identity in 571 aa overlap); Q9L3U2|STTE putative acylase from Streptomyces rochei (Streptomyces parvullus) (554 aa),FASTA scores: opt: 492, E(): 3.7e-22, (34.45% identity in 569 aa overlap); CAC49652|SMB21424 putative esterase or acylase protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (578 aa), FASTA scores: opt: 405,E(): 7.1e-17, (34.45% identity in 569 aa overlap); etc.; Possible hydrolase 3110065 888932 Rv2800 Mycobacterium tuberculosis H37Rv Possible hydrolase NP_217316.1 3108416 D 83332 CDS NP_217317.1 15609938 888921 complement(3110167..3110523) 1 NC_000962.3 Rv2801c, (MTCY16B7.42), len: 118 aa. MazF9, toxin,part of toxin-antitoxin (TA) operon with Rv2801A (See Pandey and Gerdes, 2005; Zhu et al., 2006), highly similar to Q9RWK4|DR0662 conserved hypothetical protein from Deinococcus radiodurans (115 aa), FASTA scores: opt: 306,E(): 2e-15, (43.95% identity in 116 aa overlap); and similar to AAK78474|CAC0494 PEMK family of DNA-binding proteins from Clostridium acetobutylicum (122 aa), FASTA scores: opt: 217, E(): 7.3e-09, (33.35% identity in 117 aa overlap); P96622|YDCE YDCE protein from Bacillus subtilis (116 aa), FASTA scores: opt: 194, E(): 3.5e-07, (33.35% identity in 117 aa overlap); Q9PHH8|XFA0027 plasmid maintenance protein from Xylella fastidiosa (108 aa), FASTA scores: opt: 188, E(): 9.1e-07, (40.85% identity in 115 aa overlap); etc. Also similar to Q10867|YJ91_MYCTU|Rv1991c|MT2046|MTCY39.28 hypothetical 12.3 KDA protein from Mycobacterium tuberculosis (114 aa),FASTA scores: opt: 190, E(): 6.8e-07, (36.75% identity in 117 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Toxin MazF9 3110523 mazF9 888921 mazF9 Mycobacterium tuberculosis H37Rv Toxin MazF9 NP_217317.1 3110167 R 83332 CDS YP_007411507.1 448817634 14515892 complement(3110507..3110737) 1 NC_000962.3 Rv2801A, len: 76 aa. Possible mazE9, antitoxin, part of toxin-antitoxin (TA) operon with Rv2801c (See Pandey and Gerdes, 2005; Zhu et al., 2006). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible antitoxin MazE9 3110737 mazE9 14515892 mazE9 Mycobacterium tuberculosis H37Rv Possible antitoxin MazE9 YP_007411507.1 3110507 R 83332 CDS NP_217318.1 15609939 888931 complement(3110780..3111823) 1 NC_000962.3 Rv2802c, (MTCY16B7.41), len: 347 aa. Hypothetical unknown arg-, ala-rich protein. C-terminus shows some similarity with N-terminal part of hypothetical proteins Q98K84|MLR1592 from Rhizobium loti (Mesorhizobium loti) (104 aa), FASTA scores: opt: 138, E(): 0.12, (37.35% identity in 91 aa overlap); and CAC47718|SMC03294 from Rhizobium meliloti (Sinorhizobium meliloti) (114 aa), FASTA scores: opt: 128, E(): 0.53, (31.4% identity in 86 aa overlap). Equivalent to AAK47191 from Mycobacterium tuberculosis strain CDC1551 (357 aa) but shorter 10 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical arginine and alanine rich protein 3111823 888931 Rv2802c Mycobacterium tuberculosis H37Rv Hypothetical arginine and alanine rich protein NP_217318.1 3110780 R 83332 CDS YP_177678.1 57117026 888416 3111822..3112289 1 NC_000962.3 Rv2803, len: 155 aa. Conserved hypothetical protein,similar to hypothetical proteins from other organisms, and with some similarity to C-terminal part of Rv0918|Z95210_12 hypothetical protein from Mycobacterium tuberculosis (158 aa), FASTA scores: opt: 204, E(): 9e-07, (42.35% identity in 85 aa overlap). Replaces original 2803c on other strand. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3112289 888416 Rv2803 Mycobacterium tuberculosis H37Rv hypothetical protein YP_177678.1 3111822 D 83332 CDS NP_217320.1 15609941 888418 complement(3112465..3113094) 1 NC_000962.3 Rv2804c, (MTCY16B7.39), len: 209 aa. Hypothetical unknown protein, overlaps neighbouring orf Rv2805|MTCY16B7.38c. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3113094 888418 Rv2804c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217320.1 3112465 R 83332 CDS NP_217321.1 15609942 888916 3112867..3113271 1 NC_000962.3 Rv2805, (MTCY16B7.38c), len: 134 aa. Conserved hypothetical protein, highly similar to N-terminal region of downstream ORF P71644|Rv2807|MTCY16B7.36c conserved hypothetical protein from Mycobacterium tuberculosis (384 aa), FASTA scores: opt: 525, E(): 6.4e-29, (78.2% identity in 101 aa overlap). Also highly similar to N-terminus of other proteins: Q9KK74 hypothetical 47.4 KDA protein from Brevibacterium linens (418 aa), FASTA scores: opt: 480,E(): 8.8e-26, (64.15% identity in 106 aa overlap); AAK40065 Rv3128c-like protein from Mycobacterium celatum (423 aa),FASTA scores: opt: 218, E(): 1.2e-07, (46.05% identity in 89 aa overlap); Q981U5|MLR9230 from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 131, E(): 0.15, (29.4% identity in 126 aa overlap). Overlaps neighbouring ORF Rv2804c|MTCY16B7.39. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3113271 888916 Rv2805 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217321.1 3112867 D 83332 CDS NP_217322.1 15609943 888905 3113268..3113459 1 NC_000962.3 Rv2806, (MTCY16B7.37c), len: 63 aa. Possible membrane protein, sharing no homology. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible membrane protein 3113459 888905 Rv2806 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_217322.1 3113268 D 83332 CDS NP_217323.1 15609944 888907 3113658..3114812 1 NC_000962.3 Rv2807, (MTCY16B7.36c), len: 384 aa. Conserved hypothetical protein, highly similar, but shorter 35 aa, to Q9KK74 hypothetical 47.4 KDA protein from Brevibacterium linens (418 aa), FASTA scores: opt: 1865, E(): 9.4e-116,(69.75% identity in 380 aa overlap); and with similarity with other hypothetical proteins or transposases e.g. Q981U5|MLR9230 protein from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 636,, (36.05% identity in 377 aa overlap); CAC47689 putative transposase for insertion sequence ISRM18 from Rhizobium meliloti (Sinorhizobium meliloti) (507 aa), FASTA scores: opt: 553,E(): 6.6e-29, (33.5% identity in 370 aa overlap); etc. Also similar to Rv3128c|MTCY164.38c (336 aa) (47.2% identity in 339 aa overlap); and high similarity at N-terminal region with Rv2805|MTCY16B7.38c (79.2% identity in 101 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3114812 888907 Rv2807 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217323.1 3113658 D 83332 CDS NP_217324.1 15609945 888944 3115046..3115303 1 NC_000962.3 Rv2808, (MTCY16B7.35c), len: 85 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3115303 888944 Rv2808 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217324.1 3115046 D 83332 CDS NP_217325.1 15609946 888909 3115408..3115719 1 NC_000962.3 Rv2809, (MTCY16B7.34c), len: 103 aa (questionable ORF). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 3115719 888909 Rv2809 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217325.1 3115408 D 83332 CDS NP_217326.1 15609947 887784 complement(3115741..>3116142) 1 NC_000962.3 Rv2810c, (MTCY16B7.33), len: 133 aa. Probable transposase for IS1555, similar to C-terminal domain of transposases for defective IS1555 e.g. Q9LCS0|TNPA transposase from Arthrobacter sp. TM1 (435 aa), FASTA scores: opt: 294, E(): 1.8e-13, (55.1% identity in 98 aa overlap); Q50440|TNPA insertion element TNPR and TNPA gene from Mycobacterium smegmatis (413 aa), FASTA scores: opt: 274, E(): 4.7e-12, (56.25% identity in 96 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase >3116142 887784 Rv2810c Mycobacterium tuberculosis H37Rv Probable transposase NP_217326.1 3115741 R 83332 CDS NP_217327.1 15609948 887740 3116139..3116747 1 NC_000962.3 Rv2811, (MTCY16B7.32c), len: 202 aa. Conserved hypothetical protein. C-terminus equivalent to C-terminus of AAK47198|MT2878 hypothetical 17.7 KDA protein Mycobacterium tuberculosis strain CDC1551 (178 aa), FASTA scores: opt: 609, E(): 1.5e-32, (61.0% identity in 182 aa overlap); and C-terminus highly similar to P72038|Rv3771c|MTCY13D12.05c hypothetical 11.3 KDA protein from Mycobacterium tuberculosis (108 aa), FASTA scores: opt: 465, E(): 2.8e-23, (73.6% identity in 106 aa overlap). Also some similarity with P71962|Rv2665|MTCY441.34 hypothetical 10.5 KDA protein from Mycobacterium tuberculosis (93 aa), FASTA scores: opt: 153, E(): 0.0057,(39.05% identity in 64 aa overlap); and Q9A6W6|CC1966 hypothetical protein CC1966 from Caulobacter crescentus (189 aa), FASTA scores: opt: 115, E(): 2.6, (39.4% identity in 104 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 3116747 887740 Rv2811 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217327.1 3116139 D 83332 CDS NP_217328.1 15609949 888942 3116818..3118227 1 NC_000962.3 Rv2812, (MTCY16B7.31c), len: 469 aa. Probable transposase for IS1604, similar to putative transposases and hypothetical proteins e.g. Q9EZM2|putative transposase from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 329, E(): 3e-13, (27.05% identity in 362 aa overlap); CAC46499 putative transposase protein from Rhizobium meliloti (Sinorhizobium meliloti) (390 aa), FASTA scores: opt: 327, E(): 3.9e-13, (30.5% identity in 367 aa overlap); etc. Contains possible helix-turn-helix motif at aa 50-71 (Score 1140, +3.07 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable transposase 3118227 888942 Rv2812 Mycobacterium tuberculosis H37Rv Probable transposase NP_217328.1 3116818 D 83332 CDS NP_217329.1 15609950 888570 3118224..3119036 1 NC_000962.3 Rv2813, (MTCY16B7.30c), len: 270 aa. Conserved hypothetical protein, similar to various proteins (notably secreted proteins) e.g. Q9ZFL2 hypothetical 30.4 KDA protein from Bacillus stearothermophilus (266 aa), FASTA scores: opt: 518, E(): 1.4e-26, (33.85% identity in 266 aa overlap); P45754|GSPA_AERHY|EXEA general secretion pathway protein from Aeromonas hydrophila (547 aa), FASTA scores: opt: 386, E(): 1.1e-17, (32.05% identity in 265 aa overlap); Q9KPC7|VC2445 general secretion pathway protein A from Vibrio cholerae (529 aa), FASTA scores: opt: 366, E(): 2.2e-16, (31.1% identity in 270 aa overlap); Q56674|VC0403 mannose-sensitive hemagglutinin D from Vibrio cholerae (281 aa), FASTA scores: opt: 317, E(): 2.1e-13, (27.85% identity in 262 aa overlap); etc. Also highly similar to AAK40072 Rv2813-like protein from Mycobacterium celatum (270 aa),FASTA scores: opt: 1628, E(): 2.8e-99, (90.75% identity in 270 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 3119036 888570 Rv2813 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217329.1 3118224 D 83332 CDS NP_217330.2 448824791 887839 complement(3120566..>3121552) 1 NC_000962.3 Rv2814c, (MTCY16B7.29), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2814c and Rv2815c, the sequence UUUUAAAG (directly upstream of Rv2814c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase >3121552 887839 Rv2814c Mycobacterium tuberculosis H37Rv Probable transposase NP_217330.2 3120566 R 83332 CDS NP_217331.1 15609952 888511 complement(3121501..3121827) 1 NC_000962.3 Rv2815c, (MTCY16B7.28), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2814c and Rv2815c, the sequence UUUUAAAG (directly upstream of Rv2814c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable transposase 3121827 888511 Rv2815c Mycobacterium tuberculosis H37Rv Probable transposase NP_217331.1 3121501 R 83332 CDS NP_217332.1 15609953 888520 complement(3123625..3123966) 1 NC_000962.3 Rv2816c, (MTCY16B7.27), len: 113 aa. Conserved hypothetical protein, highly similar in part to N-terminus of several proteins e.g. O28403|AF1876 conserved hypothetical protein from Archaeoglobus fulgidus (94 aa),FASTA scores: opt: 137, E(): 0.0022, (47.55% identity in 61 aa overlap); Q97Y85|SSO8090 hypothetical protein from Sulfolobus solfataricus (88 aa), FASTA scores: opt: 124,E(): 0.02, (37.3% identity in 59 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3123966 888520 Rv2816c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217332.1 3123625 R 83332 CDS NP_217333.1 15609954 888506 complement(3123967..3124983) 1 NC_000962.3 Rv2817c, (MTCY16B7.26), len: 338 aa. Conserved hypothetical protein, showing similarity with O30236|AF2435 conserved hypothetical protein from Archaeoglobus fulgidus (322 aa), FASTA scores: opt: 397, E(): 2.4e-19, (28.2% identity in 298 aa overlap); Q9KFX9|BH0341 hypothetical protein from Bacillus halodurans (343 aa), FASTA scores: opt: 337, E(): 2.8e-15, (27.35% identity in 300 aa overlap); Q9X2B7|TM1797 conserved hypothetical protein from Thermotoga maritima (319 aa), FASTA scores: opt: 321, E(): 3.3e-14, (26.5% identity in 268 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3124983 888506 Rv2817c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217333.1 3123967 R 83332 CDS NP_217334.1 15609955 888510 complement(3124996..3126144) 1 NC_000962.3 Rv2818c, (MTCY16B7.25), len: 382 aa. Hypothetical unknown protein, equivalent to AAK47210 from Mycobacterium tuberculosis strain CDC1551 (430 aa) but shorter 48 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 3126144 888510 Rv2818c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217334.1 3124996 R 83332 CDS NP_217335.1 15609956 888514 complement(3126240..3127367) 1 NC_000962.3 Rv2819c, (MTCY16B7.23), len: 375 aa. Hypothetical unknown protein (see citations below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 3127367 888514 Rv2819c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217335.1 3126240 R 83332 CDS NP_217336.1 15609957 888908 complement(3127364..3128272) 1 NC_000962.3 Rv2820c, (MTCY16B7.22), len: 302 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3128272 888908 Rv2820c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217336.1 3127364 R 83332 CDS NP_217337.1 15609958 887741 complement(3128253..3128963) 1 NC_000962.3 Rv2821c, (MTCY16B7.21), len: 236 aa. Conserved hypothetical protein, similar to several hypothetical proteins e.g. Q9X2C9|TM1809 conserved hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 318,E(): 8.2e-15, (39.45% identity in 213 aa overlap); O27152|MTH1080 conserved hypothetical protein from Methanothermobacter thermautotrophicus (245 aa), FASTA scores: opt: 294, E(): 3.9e-13, (34.8% identity in 224 aa overlap); BAB59251|TVG0114661 hypothetical protein from Thermoplasma volcanium (229 aa), FASTA scores: opt: 252,E(): 3.3e-10, (33.8% identity in 225 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3128963 887741 Rv2821c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217337.1 3128253 R 83332 CDS NP_217338.1 15609959 887778 complement(3128973..3129347) 1 NC_000962.3 Rv2822c, (MTCY16B7.20), len: 124 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3129347 887778 Rv2822c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217338.1 3128973 R 83332 CDS NP_217339.1 15609960 887735 complement(3129344..3131773) 1 NC_000962.3 Rv2823c, (MTCY16B7.19), len: 809 aa. Conserved protein, similar in part to others e.g. Q9X2D1|TM1811Thermotoga maritima (717 aa), FASTA scores: opt: 401, E(): 3.6e-18, (27.15% identity in 773 aa overlap); O27154|MTH1082 conserved hypothetical protein from Methanothermobacter thermautotrophicus (822 aa), FASTA scores: opt: 306, E(): 6e-12, (25.55% identity in 872 aa overlap); Q59066|MJ1672 hypothetical protein from Methanococcus jannaschii (800 aa), FASTA scores: opt: 302,E(): 1.1e-11, (24.9% identity in 812 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3131773 887735 Rv2823c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217339.1 3129344 R 83332 CDS NP_217340.1 15609961 888945 complement(3131770..3132714) 1 NC_000962.3 Rv2824c, (MTCY16B7.18), len: 314 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3132714 888945 Rv2824c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217340.1 3131770 R 83332 CDS NP_217341.1 15609962 887769 complement(3132892..3133539) 1 NC_000962.3 Rv2825c, (MTCY16B7.17), len: 215 aa. Conserved hypothetical protein, similar to Q9RY53|DR0097 conserved hypothetical protein from Deinococcus radiodurans (189 aa),FASTA scores: opt: 261, E(): 8e-11, (33.5% identity in 176 aa overlap); and shows some similarity with N-terminus of O27278|MTH1210 MRR restriction system related protein from Methanothermobacter thermautotrophicus (340 aa), FASTA scores: opt: 133, E(): 0.091, (28.55% identity in 112 aa overlap). Equivalent to AAK47217 from Mycobacterium tuberculosis strain CDC1551 (246 aa) but shorter 31 aa; and equivalent to upstream ORF P71624|Rv2828c|MTCY16B7.14 from Mycobacterium tuberculosis strain H37Rv (alias AAK47221 from strain CDC1551) (181 aa), FASTA scores: opt: 1169,E(): 8.5e-74, (98.35% identity in 181 aa overlap).; hypothetical protein 3133539 887769 Rv2825c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217341.1 3132892 R 83332 CDS NP_217342.1 15609963 887742 complement(3133709..3134593) 1 NC_000962.3 Rv2826c, (MTCY16B7.16), len: 294 aa. Hypothetical unknown protein.; Hypothetical protein 3134593 887742 Rv2826c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217342.1 3133709 R 83332 CDS NP_217343.1 15609964 887707 complement(3134596..3135483) 1 NC_000962.3 Rv2827c, (MTCY16B7.15), len: 295 aa. Hypothetical unknown protein, equivalent to AAK47219 from Mycobacterium tuberculosis strain CDC1551 (315 aa) but shorter 20 aa.; Hypothetical protein 3135483 887707 Rv2827c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217343.1 3134596 R 83332 CDS NP_217344.1 15609965 887749 complement(3135788..3136333) 1 NC_000962.3 Rv2828c, (MTCY16B7.14), len: 181 aa. Conserved hypothetical protein, similar to Q9RY53|DR0097 conserved hypothetical protein from Deinococcus radiodurans (189 aa),FASTA scores: opt: 267, E(): 1.9e-11, (34.1% identity in 176 aa overlap); and shows some similarity with N-terminus of O27278|MTH1210 MRR restriction system related protein from Methanothermobacter thermautotrophicus (340 aa), FASTA scores: opt: 133, E(): 0.07, (28.55% identity in 112 aa overlap). Also equivalent to downstream ORF P71627|Rv2825c|MTCY16B7.17 from Mycobacterium tuberculosis strain H37Rv (alias AAK47217 from strain CDC1551, 246 aa) (215 aa), FASTA scores: opt: 1173, E(): 8.3e-75, (98.9% identity in 181 aa overlap).; hypothetical protein 3136333 887749 Rv2828c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217344.1 3135788 R 83332 CDS YP_007411535.1 448817662 14515869 complement(3136330..3136599) 1 NC_000962.3 Rv2828A, len: 89 aa. Conserved hypothetical protein,present in many mycobacteria. Equivalent to BCG2848c and Mb2852A (100% identity to both in 89 aa overlap); hypothetical protein 3136599 14515869 Rv2828A Mycobacterium tuberculosis H37Rv hypothetical protein YP_007411535.1 3136330 R 83332 CDS NP_217345.1 15609966 887730 complement(3136620..3137012) 1 NC_000962.3 Rv2829c, (MTCY16B7.13), len: 130 aa. Possible vapC22, toxin, part of toxin-antitoxin (TA) operon with Rv2830c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Conserved hypothetical protein similar to AAK65872|SMA2253 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (125 aa),FASTA scores: opt: 171, E(): 7.7e-05, (34.9% identity in 129 aa overlap); and shows some similarity with other proteins e.g. Q9AH69 hypothetical 14.7 KDA protein from Neisseria meningitidis (128 aa), FASTA scores: opt: 148,E(): 0.0031, (28.1% identity in 121 aa overlap).; Possible toxin VapC22 3137012 vapC22 887730 vapC22 Mycobacterium tuberculosis H37Rv Possible toxin VapC22 NP_217345.1 3136620 R 83332 CDS NP_217346.1 15609967 888537 complement(3137009..3137224) 1 NC_000962.3 Rv2830c, (MTCY16B7.12), len: 71 aa. Possible vapB22,antitoxin, part of toxin-antitoxin (TA) operon with Rv2829c, (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Z97182|MTCY19H5.26|Rv0596c Hypothetical protein (85 aa),FASTA scores: opt: 88, E(): 1.3, (41.7% identity in 36 aa overlap); and to PHD_BPP1|Q06253 bacteriophage P1 phd gene (73 aa), FASTA scores: opt: 79, E(): 3.8, (35.9% identity in 39 aa overlap).; Possible antitoxin VapB22 3137224 vapB22 888537 vapB22 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB22 NP_217346.1 3137009 R 83332 CDS NP_217347.1 15609968 888519 3137271..3138020 1 NC_000962.3 Rv2831, (MTCY16B7.11c), len: 249 aa. Probable echA16, enoyl-CoA hydratase, similar to others e.g. O23468|AT4G16210 from Arabidopsis thaliana (Mouse-ear cress) (244 aa), FASTA scores: opt: 491, E(): 7.3e-25,(42.1% identity in 190 aa overlap); Q98LI4|MLL1009 from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 491, E(): 7.6e-25, (40.75% identity in 248 aa overlap); O07137|ECH8_MYCLE|ML2402|MLCB1306.05c from Mycobacterium leprae (257 aa), FASTA scores: opt: 478, E(): 5.3e-24, (38.05% identity in 226 aa overlap); P76082|PAAF_ECOLI|B1393 from scherichia coli strain K12 (255 aa), FASTA scores: opt: 439, E(): 1.9e-21, (37.55% identity in 221 aa overlap); etc. Also similar to O53418|ECH8_MYCTU|ECHA8|Rv1070c|MT1100|MTV017.23c from Mycobacterium tuberculosis (257 aa), FASTA scores: opt: 471, E(): 1.5e-23, (38.05% identity in 226 aa overlap).; Probable enoyl-CoA hydratase EchA16 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 3138020 echA16 888519 echA16 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA16 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_217347.1 3137271 D 83332 CDS NP_217348.1 15609969 888554 complement(3138099..3139181) 1 NC_000962.3 Rv2832c, (MTCY16B7.10), len: 360 aa. Probable ugpC,Sn-glycerol-3-phosphate transport ATP-binding protein ABC transporter (see Braibant et al., 2000), similar to others: CAC48805 probable glycerol-3-phosphate ABC transporter ATP-binding protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (349 aa), FASTA scores: opt: 1018,E(): 4.1e-53, (48.6% identity in 356 aa overlap); Q98G42|MLL3499|UGPC SN-glycerol-3-phosphate transport ATP-binding protein from Rhizobium loti (Mesorhizobium loti) (366 aa), FASTA scores: opt: 1016, E(): 5.6e-53,(48.5% identity in 367 aa overlap). But also highly similar to many msiK proteins, ABC transporter ATP-binding proteins possibly involved in transport of cellolbiose and maltose (see Schlosser et al., 1997) e.g. P96483|MSIK MSIK protein from Streptomyces reticuli (377 aa), FASTA scores: opt: 1277, E(): 1.9e-68, (58.05% identity in 379 aa overlap); Q9L0Q1|MSIK ABC transporter ATP-binding protein from Streptomyces coelicolor (378 aa), FASTA scores: opt: 1276,E(): 2.1e-68, (57.65% identity in 380 aa overlap); Q54333|MSIK from Streptomyces lividans (314 aa), FASTA scores: opt: 1217, E(): 5.9e-65, (63.7% identity in 292 aa overlap); and other ABC-type sugar transport proteins. Also highly similar to O53482|Rv2038c|MTV018.25c ABC-type sugar transport protein from Mycobacterium tuberculosis (357 aa),FASTA scores: opt: 1248, E(): 9.4e-67, (56.8% identity in 354 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Probable Sn-glycerol-3-phosphate transport ATP-binding protein ABC transporter UgpC 3139181 ugpC 888554 ugpC Mycobacterium tuberculosis H37Rv Probable Sn-glycerol-3-phosphate transport ATP-binding protein ABC transporter UgpC NP_217348.1 3138099 R 83332 CDS NP_217349.1 15609970 888199 complement(3139174..3140484) 1 NC_000962.3 Rv2833c, (MTCY16B7.09), len: 436 aa. Probable ugpB,Sn-glycerol-3-phosphate binding lipoprotein component of Sn-glycerol-3-phosphate transport system (see citation below), similar to various transporters substrate-binding periplasmic proteins e.g. Q9KDY2|BH1079 glycerol-3-phosphate ABC transporter (glycerol-3-phosphate binding protein) from Bacillus halodurans (459 aa), FASTA scores: opt: 357, E(): 3.1e-14, (23.4% identity in 406 aa overlap); P72397|male putative maltose-binding protein from Streptomyces coelicolor (423 aa), FASTA scores: opt: 318,E(): 7e-12, (23.7% identity in 430 aa overlap); AAK78409|CAC0429 glycerol-3-phosphate ABC-transporter periplasmic component from Clostridium acetobutylicum (447 aa), FASTA scores: opt: 305, E(): 4.5e-11, (27.15% identity in 438 aa overlap); P10904|UGPB_ECOLI|B3453 glycerol-3-phosphate-binding periplasmic protein precursor from Escherichia coli strain K12 (438 aa); etc. Contains signal sequence and appropriately positioned prokaryotic lipoprotein attachment site (PS00013).; Probable Sn-glycerol-3-phosphate-binding lipoprotein UgpB 3140484 ugpB 888199 ugpB Mycobacterium tuberculosis H37Rv Probable Sn-glycerol-3-phosphate-binding lipoprotein UgpB NP_217349.1 3139174 R 83332 CDS NP_217350.1 15609971 888534 complement(3140487..3141314) 1 NC_000962.3 Rv2834c, (MTCY16B7.08), len: 275 aa. Probable ugpE,Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter (see citation below), similar to various permeases e.g. Q9KDY3|BH1078 glycerol-3-phosphate ABC transporter from Bacillus halodurans (270 aa), FASTA scores: opt: 620, E(): 4.3e-32, (34.7% identity in 268 aa overlap); Q9X0K6|TM1122 glycerol-3-phosphate ABC transporter permease protein from Thermotoga maritima (276 aa), FASTA scores: opt: 605, E(): 3.9e-31, (32.5% identity in 274 aa overlap); AAG58557|UGPE SN-glycerol 3-phosphate transport system (integral membrane protein) from Escherichia coli strain O157:H7 and EDL933 (281 aa), FASTA scores: opt: 574, E(): 3.7e-29, (32.95% identity in 264 aa overlap); P10906|UGPE_ECOLI|B3451 SN-glycerol-3-phosphate transport system permease protein from Escherichia coli strain K12 (281 aa), FASTA scores: opt: 569, E(): 7.6e-29,(32.6% identity in 264 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature.; Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpE 3141314 ugpE 888534 ugpE Mycobacterium tuberculosis H37Rv Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpE NP_217350.1 3140487 R 83332 CDS NP_217351.1 15609972 888569 complement(3141311..3142222) 1 NC_000962.3 Rv2835c, (MTCY1B7.07), len: 303 aa. Probable ugpA,Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter (see citation below), similar to various permeases e.g. Q9RK71|SCF11.19 probable sugar transporter inner membrane protein from Streptomyces coelicolor (316 aa), FASTA scores: opt: 643, E(): 3.1e-35, (38.85% identity in 291 aa overlap); Q9KDY4|BH1077 glycerol-3-phosphate ABC transporter (permease) from Bacillus halodurans (315 aa),FASTA scores: opt: 548, E(): 6.2e-29, (31.5% identity in 295 aa overlap); AAK78407|CAC0427 glycerol-3-phosphate ABC-transporter, permease component from Clostridium acetobutylicum (304 aa), FASTA scores: opt: 538, E(): 2.8e-28, (29.1% identity in 292 aa overlap); etc. Contains PS00062 Aldo/keto reductase family signature 2, and PS00402 Binding-protein-dependent transport systems inner membrane comp signature.; Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpA 3142222 ugpA 888569 ugpA Mycobacterium tuberculosis H37Rv Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpA NP_217351.1 3141311 R 83332 CDS NP_217352.1 15609973 888547 complement(3142309..3143628) 1 NC_000962.3 Rv2836c, (MTCY16B7.06), len: 439 aa. Possible dinF,DNA-damage-inducible protein F, integral membrane protein,similar to others e.g. BAB38450|ECS5027|AAG59243 from Escherichia coli strain O157:H7 (459 aa), FASTA scores: opt: 501, E(): 2.7e-21, (29.55% identity in 443 aa overlap); P28303|DINF_ECOLI|B4044 from Escherichia coli strain K12 (459 aa), FASTA scores: opt: 491, E(): 1e-20,(29.35% identity in 443 aa overlap); Q98B90|MLR5680 from Rhizobium loti (Mesorhizobium loti) (471 aa), FASTA scores: opt: 466, E(): 2.7e-19, (30.7% identity in 433 aa overlap); etc. But also similar or highly similar to other hypothetical proteins e.g. Q9X8U6|SCH24.32c hypothetical 46.3 KDA protein from Streptomyces coelicolor (448 aa),FASTA scores: opt: 981, E(): 1.1e-48, (42.35% identity in 437 aa overlap). Contains PS00213 Lipocalin signature.; Possible DNA-damage-inducible protein F DinF 3143628 dinF 888547 dinF Mycobacterium tuberculosis H37Rv Possible DNA-damage-inducible protein F DinF NP_217352.1 3142309 R 83332 CDS NP_217353.1 15609974 888920 complement(3143635..3144645) 1 NC_000962.3 Rv2837c, (MTCY16B7.05), len: 336 aa. Conserved protein, showing some similarity with other proteins e.g. O67552|AQ_1630 hypothetical 36.2 KDA protein from Aquifex aeolicus (325 aa), FASTA scores: opt: 498, E(): 3.6e-25,(32.8% identity in 314 aa overlap); Q9X1T1|TM1595 conserved hypothetical protein from Thermotoga maritima (333 aa),FASTA scores: opt: 482, E(): 4.1e-24, (34.85% identity in 304 aa overlap); Q9RW43|DR0826 conserved hypothetical protein from Deinococcus radiodurans (338 aa), FASTA scores: opt: 444, E(): 1.3e-21, (33.85% identity in 331 aa overlap); etc. Equivalent to AAK47229 from Mycobacterium tuberculosis strain CDC1551 (316 aa) but longer 20 aa.; hypothetical protein 3144645 888920 Rv2837c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217353.1 3143635 R 83332 CDS NP_217354.1 15609975 888923 complement(3144620..3145171) 1 NC_000962.3 Rv2838c, (MTCY16B7.04), len: 183 aa. Probable rbfA,ribosome-binding factor A, equivalent to Q9Z5I8|RBFA_MYCLE|ML1555|MLCB596.15 probable ribosome-binding factor a from Mycobacterium leprae (164 aa), FASTA scores: opt: 739, E(): 1.8e-40, (75.6% identity in 160 aa overlap). Also highly similar or similar to others e.g. Q9Z527|RBFA_STRCO|SC9F2.08c from Streptomyces coelicolor (160 aa), FASTA scores: opt: 425, E(): 2.8e-20,(50.35% identity in 141 aa overlap); P32731|RBFA_BACSU from Bacillus subtilis (117 aa), FASTA scores: opt: 199, E(): 7.8e-06, (32.4% identity in 108 aa overlap); P09170|RBFA_ECOLI|P15B|B3167 from Escherichia coli strain K12 (132 aa), FASTA scores: opt: 166, E(): 0.0011, (29.65% identity in 118 aa overlap); etc. Belongs to the RBFA family. Note that appears to be longer in C-terminus than other RbfA proteins.; Probable ribosome-binding factor a RbfA (P15B protein) 3145171 rbfA 888923 rbfA Mycobacterium tuberculosis H37Rv Probable ribosome-binding factor a RbfA (P15B protein) NP_217354.1 3144620 R 83332 CDS NP_217355.1 15609976 888157 complement(3145171..3147873) 1 NC_000962.3 Rv2839c, (MTCY16B7.03), len: 900 aa. Probable infB,translation initiation factor if-2, highly similar, but in part, to Q9Z5I9|IF2_MYCLE|ML1556|MLCB596.14 translation initiation factor if-2 from Mycobacterium leprae (924 aa),FASTA scores: opt: 4548, E(): 2.4e-132, (83.6% identity in 933 aa overlap). Also similar in part to others e.g. Q9K3E2|SC5H4.30 from Streptomyces coelicolor (835 aa),FASTA scores: opt: 2559, E(): 1.3e-71, (59.9% identity in 833 aa overlap); P17889|IF2_BACSU|INFB from Bacillus subtilis (716 aa), FASTA scores: opt: 1782, E(): 6.6e-48,(46.65% identity in 686 aa overlap); P02995|IF2_ECOLI|INFB|SSYG|B3168|Z4529|ECS4049 from Escherichia coli strains O157:H7 and K12 (890 aa), FASTA scores: opt: 1708, E(): 1.3e-45, (46.2% identity in 662 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the if-2 family.; Probable translation initiation factor if-2 InfB 3147873 infB 888157 infB Mycobacterium tuberculosis H37Rv Probable translation initiation factor if-2 InfB NP_217355.1 3145171 R 83332 CDS NP_217356.3 448824792 888189 complement(3147959..3148258) 1 NC_000962.3 Rv2840c, (MTCY16B7.02), len: 99 aa. Conserved hypothetical protein, equivalent to Q9Z5J0|ML1557|MLCB596.13 hypothetical 11.6 KDA protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 501, E(): 2.3e-29, (501% identity in 96 aa overlap). Also highly similar to other hypothetical proteins e.g. Q9KYR0|SC5H4.29 from Streptomyces coelicolor (101 aa), FASTA scores: opt: 256, E(): 1.4e-11, (50.6% identity in 81 aa overlap); Q9APM9 from Myxococcus xanthus (111 aa), FASTA scores: opt: 174, E(): 1.3e-05, (42.25% identity in 97 aa overlap); and similar to to others e.g. N-terminus of CAC41675|SMC02913 from Rhizobium meliloti (Sinorhizobium meliloti) (230 aa),FASTA scores: opt: 172, E(): 3e-05, (42.4% identity in 66 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 3148258 888189 Rv2840c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217356.3 3147959 R 83332 CDS NP_217357.1 15609978 888213 complement(3148385..3149428) 1 NC_000962.3 Rv2841c, (MTCY24A1.16, MTCY16B7.01), len: 347 aa. Probable nusA, N-utilization substance protein A,equivalent to Q9Z5J1|NUSA|ML1558 probable transcription termination/antitermination factor from Mycobacterium leprae (347 aa), FASTA scores: opt: 2054, E(): 5.4e-120,(91.95% identity in 347 aa overlap). Also highly similar to others e.g. Q9KYR1|SC5H4.28 putative transcriptional termination/antitermination factor from Streptomyces coelicolor (340 aa), FASTA scores: opt: 1346, E(): 4.3e-76,(63.35% identity in 341 aa overlap); P32727|NUSA_BACSU N utilization substance protein A (371 aa), FASTA scores: opt: 847, E(): 4.1e-45, (43.95% identity in 346 aa overlap); Q9KA74|NUSA|BH2416 transcriptional terminator from Bacillus halodurans (382 aa), FASTA scores: opt: 846,E(): 4.8e-45, (43.15% identity in 373 aa overlap); etc. Belongs to the NUSA family.; Probable N utilization substance protein A NusA 3149428 nusA 888213 nusA Mycobacterium tuberculosis H37Rv Probable N utilization substance protein A NusA NP_217357.1 3148385 R 83332 CDS NP_217358.1 15609979 888159 complement(3149425..3149976) 1 NC_000962.3 Rv2842c, (MTCY24A1.15), len: 183 aa. Conserved protein, similar to Q9Z5J2|MLCB596.11 hypothetical 13.7 KDA protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 192, E(): 2.1e-12, (50.0% identity in 128 aa overlap) (N-terminus shorter). Also similar in part to several hypothetical proteins e.g. Q9KYR2|SC5H4.27 hypothetical 19.8 KDA protein from Streptomyces coelicolor (177 aa),FASTA scores: opt: 288, E(): 2.1e-12, (37.15% identity in 148 aa overlap); O66619|Y260_AQUAE|AQ_260 hypothetical protein from Aquifex aeolicus (158 aa), FASTA scores: opt: 230, E(): 1.7e-08, (31.35% identity in 153 aa overlap); Q9KU82|VC0641 hypothetical protein from Vibrio cholerae (151 aa), FASTA scores: opt: 198, E(): 2.5e-06, (30.9% identity in 152 aa overlap); etc.; hypothetical protein 3149976 888159 Rv2842c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217358.1 3149425 R 83332 CDS NP_217359.1 15609980 888522 3150171..3150716 1 NC_000962.3 Rv2843, (MTCY24A1.14c), len: 181 aa. Probable conserved transmembrane ala-rich protein, equivalent to Q9Z5J3|ML1560|MLCB596.10c hypothetical 17.5 KDA protein from Mycobacterium leprae (178 aa), FASTA scores: opt: 707,E(): 1.4e-32, (70.25% identity in 168 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane alanine rich protein 3150716 888522 Rv2843 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane alanine rich protein NP_217359.1 3150171 D 83332 CDS NP_217360.1 15609981 888536 3150713..3151201 1 NC_000962.3 Rv2844, (MTCY24A1.13c), len: 162 aa. Conserved ala-rich protein, equivalent to Q9Z5J4|ML1561|MLCB596.09c hypothetical 17.5 KDA protein from Mycobacterium leprae (165 aa), FASTA scores: opt: 771, E(): 4.9e-46, (71.5% identity in 165 aa overlap). Also similar to Q9KYR4|SC5H4.25c hypothetical 16.8 KDA protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 242,E(): 1.6e-09, (38.9% identity in 144 aa overlap).; Conserved alanine rich protein 3151201 888536 Rv2844 Mycobacterium tuberculosis H37Rv Conserved alanine rich protein NP_217360.1 3150713 D 83332 CDS NP_217361.1 15609982 888539 complement(3151202..3152950) 1 NC_000962.3 Rv2845c, (MTCY24A1.12), len: 582 aa. Probable proS,prolyl-tRNA synthetase, highly similar to others e.g. Q9KYR6|SYP_STRCO|pros|SC5H4.23 from Streptomyces coelicolor (567 aa), FASTA scores: opt: 1161, E(): 9e-64, (57.15% identity in 574 aa overlap); P56124|SYP_HELPY|pros|HP0238 from Helicobacter pylori (Campylobacter pylori) (577 aa),FASTA scores: opt: 1082, E(): 6.6e-59, (37.8% identity in 553 aa overlap); P16659|SYP_ECOLI|pros|DRPA|B0194 from Escherichia coli strain K12 (572 aa), FASTA scores: opt: 926, E(): 2.6e-49, (39.85% identity in 587 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family.; Probable prolyl-tRNA synthetase ProS (proline--tRNA ligase) (PRORS) (global RNA synthesis factor) (proline translase) 3152950 proS 888539 proS Mycobacterium tuberculosis H37Rv Probable prolyl-tRNA synthetase ProS (proline--tRNA ligase) (PRORS) (global RNA synthesis factor) (proline translase) NP_217361.1 3151202 R 83332 CDS NP_217362.1 15609983 888575 complement(3153039..3154631) 1 NC_000962.3 Rv2846c, (MTCY24A1.11), len: 530 aa. Possible efpA,integral membrane efflux protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug (see citations below), equivalent to Q9Z5J5|ML1562|MLCB596.08 putative transmembrane efflux protein from Mycobacterium leprae (534 aa), FASTA scores: opt: 2881, E(): 4.1e-160, (86.55% identity in 535 aa overlap). Also highly similar to several membrane proteins e.g. O69986|SC4H2.31c transmembrane efflux protein (515 aa), FASTA scores: opt: 1063, E(): 2.2e-54, (39.65% identity in 406 aa overlap); Q9FBQ5|SCD86A.02c putative transport integral membrane protein from Streptomyces coelicolor (503 aa), FASTA scores: opt: 918, E(): 5.8e-46,(33.7% identity in 469 aa overlap); Q9KYU0|SCE22.23c putative transmembrane efflux protein from Streptomyces coelicolor (514 aa), FASTA scores: opt: 888, E(): 3.3e-44,(32.85% identity in 469 aa overlap); etc.; Possible integral membrane efflux protein EfpA 3154631 efpA 888575 efpA Mycobacterium tuberculosis H37Rv Possible integral membrane efflux protein EfpA NP_217362.1 3153039 R 83332 CDS NP_215025.2 57117027 887481 complement(3154654..3155871) 1 NC_000962.3 Rv2847c, (MTCY24A1.10), len: 405 aa. Possible cysG,multifunctional enzyme, siroheme synthase containing uroporphyrin-III c-methyltransferase, precorrin-2 oxidase and ferrochelatase. C-terminus highly similar to many uroporphyrin-III c-methyltransferases e.g. Q51720|COBA uroporphyrinogen III methyltransferase from Propionibacterium freudenreichii (257 aa), FASTA scores: opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa overlap); Q9HMY4|UROM|VNG2331G S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Halobacterium sp. strain NRC-1 (246 aa), FASTA scores: opt: 704, E(): 3.1e-35, (49.4% identity in 245 aa overlap); P42437|NASF_BACSU|NASBE uroporphyrin-III C-methyltransferase from Bacillus subtilis (483 aa), FASTA scores: opt: 610, E(): 2.4e-29, (42.1% identity in 240 aa overlap); etc. And highly similar over entire length to other proteins e.g. Q9L1C9|SCL11.09c uroporphyrinogen III methyltransferase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1481, E(): 5.6e-82,(58.45% identity in 409 aa overlap); Q9I0M7|CYSG|PA2611 siroheme synthase from Pseudomonas aeruginosa (465 aa),FASTA scores: opt: 609, E(): 2.7e-29, (34.7% identity in 444 aa overlap); P11098|CYSG_ECOLI|B3368|Z4729|ECS4219 siroheme synthase from Escherichia coli stains O157:H7 and K12 (457 aa), FASTA scores: opt: 543, E(): 9.1e-27, (31.3% identity in 450 aa overlap); etc. Belongs to a family that groups SUMT, CYSG, CBIF/COBM and CBIL/COBI. Note that previously known as cysG2.; Possible multifunctional enzyme siroheme synthase CysG: uroporphyrin-III C-methyltransferase (urogen III methylase) (SUMT) (uroporphyrinogen III methylase) (UROM) + precorrin-2 oxidase + ferrochelatase 3155871 cysG 887481 cysG Mycobacterium tuberculosis H37Rv Possible multifunctional enzyme siroheme synthase CysG: uroporphyrin-III C-methyltransferase (urogen III methylase) (SUMT) (uroporphyrinogen III methylase) (UROM) + precorrin-2 oxidase + ferrochelatase NP_215025.2 3154654 R 83332 CDS NP_217364.1 15609985 888499 complement(3156148..3157521) 1 NC_000962.3 Rv2848c, (MTCY24A1.09), len: 457 aa. Probable cobB,cobyrinic acid A,C-diamide synthase, highly similar to others e.g. O27509|COBB_METTH|MTH1460 from Methanobacterium thermoautotrophicum (447 aa), FASTA scores: opt: 980, E(): 1.3e-49, (39.65% identity in 454 aa overlap); Q9KBM8|BH1898 from Bacillus halodurans (465 aa), FASTA scores: opt: 928,E(): 1.4e-46, (37.0% identity in 457 aa overlap); O68108|COBB_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (435 aa), FASTA scores: opt: 921, E(): 3.3e-46, (39.35% identity in 437 aa overlap); etc. Belongs to the COBB/COBQ family, COBB subfamily.; Probable cobyrinic acid A,C-diamide synthase CobB 3157521 cobB 888499 cobB Mycobacterium tuberculosis H37Rv Probable cobyrinic acid A,C-diamide synthase CobB NP_217364.1 3156148 R 83332 CDS YP_177908.1 57117028 888236 complement(3157521..3158144) 1 NC_000962.3 Rv2849c, (MTCY24A1.08), len: 207 aa. Probable cobO,cob(I)alamin adenosyltransferase, highly similar to Q9RJ17|COBO from Streptomyces coelicolor (199 aa), FASTA scores: opt: 918, E(): 1.1e-55, (64.75% identity in 207 aa overlap); and similar to others e.g. O30785|COBO from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (212 aa), FASTA scores: opt: 329, E(): 2.8e-15, (44.3% identity in 185 aa overlap); P29930|COBO_PSEDE from Pseudomonas denitrificans (213 aa), FASTA scores: opt: 280, E(): 6.5e-12, (38.9% identity in 185 aa overlap); P31570|BTUR_SALTY|COBA from Salmonella typhimurium (196 aa), FASTA scores: opt: 278, E(): 8.4e-12, (39.8% identity in 196 aa overlap); etc. Cofactor: manganese. Note that previously known as cobA.; Probable cob(I)alamin adenosyltransferase CobO (corrinoid adenosyltransferase) (corrinoid adotransferase activity) 3158144 cobO 888236 cobO Mycobacterium tuberculosis H37Rv Probable cob(I)alamin adenosyltransferase CobO (corrinoid adenosyltransferase) (corrinoid adotransferase activity) YP_177908.1 3157521 R 83332 CDS NP_217366.1 15609987 887364 complement(3158165..3160054) 1 NC_000962.3 Rv2850c, (MTCY24A1.07), len: 629 aa. Possible magnesium-chelatase, highly similar (but with gaps) to magnesium-chelatases from notably photosynthetic organisms involved in chlorophyll biosynthesis e.g. Q9RJ18|SCI8.35c putative chelatase from Streptomyces coelicolor (672 aa),FASTA scores: opt: 1941, E(): 2.1e-85, (54.65% identity in 675 aa overlap); Q9HZQ5|PA2942 probable magnesium chelatase from Pseudomonas aeruginosa (338 aa), FASTA scores: opt: 991, E(): 2.7e-40, (49.45% identity in 368 aa overlap); O33549|BCHI mg protoporphyrin IX chelatase subunit from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (334 aa), FASTA scores: opt: 833, E(): 9.4e-33, (50.65% identity in 318 aa overlap); O30819|BCHI_RHOSH magnesium-chelatase 38 KDA subunit from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (334 aa), FASTA scores: opt: 828, E(): 1.6e-32, (50.3% identity in 318 aa overlap); etc. Equivalent to AAK47242 from Mycobacterium tuberculosis strain CDC1551 (610 aa) but longer 19 aa. COULB belong to the mg-chelatase subunits D/I family.; Possible magnesium chelatase 3160054 887364 Rv2850c Mycobacterium tuberculosis H37Rv Possible magnesium chelatase NP_217366.1 3158165 R 83332 CDS NP_217367.1 15609988 887664 complement(3160051..3160521) 1 NC_000962.3 Rv2851c, (MTCY24A1.06), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similar to others e.g. Q9KP14|VC2565 ELAA protein from Vibrio cholerae (149 aa), FASTA scores: opt: 360, E(): 1e-18, (46.05% identity in 139 aa overlap); Q9I717|PA0115 hypothetical protein from Pseudomonas aeruginosa (150 aa),FASTA scores: opt: 341, E(): 2.4e-17, (43.65% identity in 142 aa overlap); Q9K8M4|BH2982 hypothetical protein from Bacillus halodurans (155 aa), FASTA scores: opt: 320, E(): 8e-16, (40.85% identity in 142 aa overlap); P52077|ELAA_ECOLI|B2267 protein ELAA from Escherichia coli strain K12 (153 aa), FASTA scores: opt: 269, E(): 3.8e-12,(35.7% identity in 140 aa overlap); etc.; GCN5-related N-acetyltransferase 3160521 887664 Rv2851c Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_217367.1 3160051 R 83332 CDS NP_217368.1 15609989 888544 complement(3160580..3162061) 1 NC_000962.3 Rv2852c, (MT2918, MTCY24A1.05), len: 493 aa. Probable mqo, malate:quinone oxidoreductase, highly similar to others e.g. O69282|MQO_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (499 aa), FASTA scores: opt: 1701, E(): 1.2e-101, (50.7% identity in 495 aa overlap); Q9Z9Q7|BH3960 from Bacillus halodurans (500 aa),FASTA scores: opt: 1632, E(): 3.3e-97, (48.55% identity in 486 aa overlap); Q9HYF4|MQOA|PA3452 from Pseudomonas aeruginosa (523 aa), FASTA scores: opt: 1604, E(): 2.1e-95,(49.1% identity in 487 aa overlap) (N-terminus longer); P33940|MQO_ECOLI|B2210 from Escherichia coli strain K12 (548 aa), FASTA scores: opt: 1525, E(): 2.7e-90, (48.15% identity in 492 aa overlap); etc. Belongs to the MQO family. Cofactors: FAD.; Probable malate:quinone oxidoreductase Mqo (malate dehydrogenase [acceptor]) 3162061 mqo 888544 mqo Mycobacterium tuberculosis H37Rv Probable malate:quinone oxidoreductase Mqo (malate dehydrogenase [acceptor]) NP_217368.1 3160580 R 83332 CDS YP_177909.1 57117029 888171 3162268..3164115 1 NC_000962.3 Rv2853, (MTCY24A1.04c), len: 615 aa. PE_PGRS48,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many e.g. O53884|Rv0872c|MTV043.65c from Mycobacterium tuberculosis (606 aa), FASTA scores: opt: 1405, E(): 1.4e-97, (64.6% identity in 619 aa overlap). Equivalent to AAK47245 from Mycobacterium tuberculosis strain CDC1551 (663 aa) but shorter 48 aa.; PE-PGRS family protein PE_PGRS48 3164115 PE_PGRS48 888171 PE_PGRS48 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS48 YP_177909.1 3162268 D 83332 CDS NP_217370.1 15609991 888542 3164152..3165192 1 NC_000962.3 Rv2854, (MTCY24A1.03c), len: 346 aa. Unknown protein, showing similarity with Q9CD03|ML2603 hypothetical protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 154, E(): 0.0083, (33.35% identity in 87 aa overlap).; hypothetical protein 3165192 888542 Rv2854 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217370.1 3164152 D 83332 CDS YP_177910.1 57117030 887773 3165205..3166584 1 NC_000962.3 Rv2855, (MTCY24A1.02c), len: 459 aa. Mtr,NADPH-dependent mycothiol reductase, proven enzymatically but previously described as glutathione reductase homolog (gene name: gorA) (see citation below). Similar to others e.g. Q9L7K8|MERA mercuric reductase from Streptomyces sp. CHR28 (474 aa), FASTA scores: opt: 719, E(): 9e-38, (35.2% identity in 460 aa overlap); P30341|MERA_STRLI mercuric reductase from Streptomyces lividans (474 aa), FASTA scores: opt: 712, E(): 2.5e-37, (34.95% identity in 455 aa overlap); Q98ED5|MLL4296 ferric leghemoglobin reductase-2 precursor, dihydrolipoamide dehydrogenase from Rhizobium loti (Mesorhizobium loti) (468 aa), FASTA scores: opt: 670,E(): 1.1e-34, (30.8% identity in 471 aa overlap); etc. Belongs to the pyridine nucleotide-disulphide oxidoreductases class-I. Cofactor: FAD.; NADPH-dependent mycothiol reductase Mtr 3166584 mtr 887773 mtr Mycobacterium tuberculosis H37Rv NADPH-dependent mycothiol reductase Mtr YP_177910.1 3165205 D 83332 CDS NP_217372.1 15609993 888643 3166684..3167802 1 NC_000962.3 Rv2856, (MTCY24A1.01c), len: 372 aa. Possible nicT,nickel-transport integral membrane protein, similar to transport proteins and hydrogenase cluster proteins e.g. BAB58860|SAV2698 hypothetical 37.9 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (338 aa), FASTA scores: opt: 1082, E(): 7.1e-60, (48.05% identity in 335 aa overlap); Q97ZB2|HOXN high-affinity nickel-transport protein from Sulfolobus solfataricus (373 aa), FASTA scores: opt: 922, E(): 6.6e-50, (42.2% identity in 372 aa overlap); P23516|HOXN_ALCEU high-affinity nickel transport protein (integral membrane protein) from Alcaligenes eutrophus (Ralstonia eutropha) (351 aa), FASTA scores: opt: 904, E(): 8.3e-49, (41.9% identity in 339 aa overlap); Q45247|HUPN_BRAJA hydrogenase nickel incorporation protein from Bradyrhizobium japonicum (381 aa), FASTA scores: opt: 853, E(): 1.3e-45, (41.65% identity in 329 aa overlap); etc. Seems to belong to the HOXN/HUPN/NIXA family of nickel transporters (NiCoT family).; Possible nickel-transport integral membrane protein NicT 3167802 nicT 888643 nicT Mycobacterium tuberculosis H37Rv Possible nickel-transport integral membrane protein NicT NP_217372.1 3166684 D 83332 CDS NP_217373.1 15609994 888560 complement(3168583..3169359) 1 NC_000962.3 Rv2857c, (MTV003.03c), len: 258 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases e.g. O88068|SCI35.33c probable dehydrogenase (SDR family) from Streptomyces coelicolor (260 aa), FASTA scores: opt: 1208, E(): 2e-68, (72.35% identity in 253 aa overlap); Q9I376|PA1649 from Pseudomonas aeruginosa probable short-chain dehydrogenase (253 aa),FASTA scores: opt: 569, E(): 2.1e-28, (39.2% identity in 255 aa overlap); Q9EX74|MLHA SDR-like enzyme from Rhodococcus erythropolis (246 aa), FASTA scores: opt: 567,E(): 2.8e-28, (41.15% identity in 248 aa overlap); etc. Also similar to many Mycobacterium tuberculosis dehydrogenases e.g. FABG3|Rv2002|MT2058|MTCY39.16c putative oxidoreductase (260 aa), FASTA score: (38.3% identity in 248 aa overlap). Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 3169359 888560 Rv2857c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_217373.1 3168583 R 83332 CDS NP_217374.1 15609995 888636 complement(3169356..3170723) 1 NC_000962.3 Rv2858c, (MTV003.04c), len: 455 aa. Probable aldC,aldehyde dehydrogenase, similar to many e.g. O88069|SCI35.34c putative aldehyde dehydrogenase from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1872,E(): 6.4e-109, (64.5% identity in 448 aa overlap); Q9FAB1|ALDH|BT-ALDH aldehyde dehydrogenase from Bacillus thermoleovorans (497 aa), FASTA scores: opt: 1157, E(): 2.1e-64, (44.3% identity in 458 aa overlap); O33455|CYMC P-CUMIC aldehyde dehydrogenase from Pseudomonas putida (494 aa), FASTA scores: opt: 1149, E(): 6.5e-64, (43.15% identity in 452 aa overlap); P40047|DHA5_YEAST|ALD5|ALDH5|ALD3|YER073W aldehyde dehydrogenase from Saccharomyces cerevisiae (Baker's yeast) (519 aa), FASTA scores: opt: 1091, E(): 2.7e-60, (38.55% identity in 459 aa overlap); P80668|FEAB_ECOLI|PADA|MAOB|B1385 phenylacetaldehyde dehydrogenase from Escherichia coli strain K12 (499 aa),FASTA scores: opt: 1074, E(): 3e-59, (42.2% identity in 462 aa overlap); etc. Also similar to many M. tuberculosis dehydrogenases e.g. P71823|Rv0768|MTCY369.13 (489 aa),FASTA score: (38.1% identity in 467 aa overlap). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family.; Probable aldehyde dehydrogenase AldC 3170723 aldC 888636 aldC Mycobacterium tuberculosis H37Rv Probable aldehyde dehydrogenase AldC NP_217374.1 3169356 R 83332 CDS NP_217375.1 15609996 887495 complement(3170720..3171646) 1 NC_000962.3 Rv2859c, (MTV003.05c), len: 308 aa. Possible amidotransferase, equivalent (but longer 58 aa) to Q9CBU9|ML1573 possible amidotransferase from Mycobacterium leprae (249 aa), FASTA scores: opt: 1226, E(): 3e-64,(71.55% identity in 239 aa overlap). Also similar to other amidotransferases and hypothetical proteins, but shorter in N-terminus e.g. O88072|SCI35.37 hypothetical 25.3 KDA protein from Streptomyces coelicolor (242 aa), FASTA scores: opt: 683, E(): 1.2e-32, (47.65% identity in 235 aa overlap); AAK79730|Q97I88|CAC1764 predicted glutamine amidotransferase from Clostridium acetobutylicum (241 aa),FASTA scores: opt: 458, E(): 1.6e-19, (32.95% identity in 246 aa overlap); AAK75201|Q97QV9|SP1089 glutamine amidotransferase class I from Streptococcus pneumoniae (229 aa), FASTA scores: opt: 431, E(): 5.6e-18, (34.75% identity in 236 aa overlap); etc. Contains three 17 aa repeats at the N-terminus very similar to those in other Mycobacterium tuberculosis proteins e.g. Q10699|YY30_MYCTU|Rv2090|MT2151|MTCY49.30 putative 5'-3' exonuclease RV2090.; Possible amidotransferase 3171646 887495 Rv2859c Mycobacterium tuberculosis H37Rv Possible amidotransferase NP_217375.1 3170720 R 83332 CDS NP_217376.1 15609997 887420 complement(3171627..3173000) 1 NC_000962.3 Rv2860c, (MTV003.06c), len: 457 aa. Probable glnA4,glutamine synthetase class II, similar to many glutamine synthases e.g. O88070|SCI35.35c from Streptomyces coelicolor (462 aa), FASTA scores: opt: 1947, E(): 8.2e-120, (64.15% identity in 452 aa overlap); Q98H15|MLL3074 from Rhizobium loti (Mesorhizobium loti) (465 aa), FASTA scores: opt: 1321, E(): 7.8e-79, (46.7% identity in 452 aa overlap); Q98EM0|MLL4187 from Rhizobium loti (Mesorhizobium loti) (456 aa), FASTA scores: opt: 698,E(): 4.6e-38, (33.5% identity in 454 aa overlap); Q9CDL9|GLNA from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (446 aa), FASTA scores: opt: 633,E(): 8.2e-34, (32.45% identity in 456 aa overlap); etc. Also similar to three other potential glutamine synthases in Mycobacterium tuberculosis: Q10378|GLN2_MYCTU|GLNA2|Rv2222c|MT2280|MTCY190.33c|MTCY427 .03c probable glutamine synthetase (446 aa), FASTA score: (31.1% identity in 453 aa overlap); Rv1878|glnA3 and Rv2220|glnA1. Belongs to the glutamine synthetase family.; Probable glutamine synthetase GlnA4 (glutamine synthase) (GS-II) 3173000 glnA4 887420 glnA4 Mycobacterium tuberculosis H37Rv Probable glutamine synthetase GlnA4 (glutamine synthase) (GS-II) NP_217376.1 3171627 R 83332 CDS YP_177911.1 57117031 888596 complement(3173160..3174017) 1 NC_000962.3 Rv2861c, (MT2929, MTV003.07c), len: 285 aa. mapB (alternate gene name: map), methionine aminopeptidase,equivalent to Q9CBU7|MAPB|ML1576 methionine aminopeptidase from Mycobacterium leprae (285 aa), FASTA scores: opt: 1729, E(): 1e-99, (89.75% identity in 283 aa overlap). Also highly similar to many e.g. Q9RKR2|MAP3 from Streptomyces coelicolor (285 aa), FASTA scores: opt: 1385, E(): 2e-78,(70.65% identity in 283 aa overlap); Q9SW64|C7A10.320|AT4G37040 from Arabidopsis thaliana (Mouse-ear cress) (305 aa), FASTA scores: opt: 914, E(): 3e-49, (50.35% identity in 286 aa overlap); P07906|AMPM_ECOLI|map|B0168|Z0178|ECS0170 from Escherichia coli strains K12 and O157:H7 (264 aa), FASTA scores: opt: 793, E(): 8.5e-42, (51.0% identity in 245 aa overlap); etc. Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Note that this gene has an N-terminal extension present in the human map, but not in the prokaryotic map's. An alternative start, with RBS, will give a protein equivalent to the shorter prokaryotic map's. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Methionine aminopeptidase MapB (map) (peptidase M) 3174017 mapB 888596 mapB Mycobacterium tuberculosis H37Rv Methionine aminopeptidase MapB (map) (peptidase M) YP_177911.1 3173160 R 83332 CDS NP_217378.1 15609999 887423 complement(3174059..3174643) 1 NC_000962.3 Rv2862c, (MTV003.08), len: 194 aa. Conserved hypothetical protein, showing some similarity with others e.g. Q9X8X5|SCH35.31c hypothetical 19.6 KDA protein from Streptomyces coelicolor (180 aa), FASTA scores: opt: 266,E(): 2.2e-11, (34.65% identity in 179 aa overlap); Q9Z5H1|ML0169|MLCB373.19 hypothetical 22.1 KDA protein from Mycobacterium leprae (200 aa), FASTA scores: opt: 195, E(): 2.3e-06, (30.15% identity in 189 aa overlap); etc. Also some similarity to P71544|Y966_MYCTU|Rv0966c|MT0994|MTCY10D7.08 conserved hypothetical protein from Mycobacterium tuberculosis (230 aa), FASTA scores: opt: 209, E(): 2.6e-07, (31.5% identity in 184 aa overlap).; hypothetical protein 3174643 887423 Rv2862c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217378.1 3174059 R 83332 CDS YP_007411570.1 448817697 14515870 3174747..3174995 1 NC_000962.3 Rv2862A, len: 82 aa. Possible vapB23, antitoxin,part of toxin-antitoxin (TA) operon with Rv2863 (See Pandey and Gerdes, 2005).; Possible antitoxin VapB23 3174995 vapB23 14515870 vapB23 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB23 YP_007411570.1 3174747 D 83332 CDS NP_217379.1 15610000 888195 3174992..3175372 1 NC_000962.3 Rv2863, (MTV003.09), len: 126 aa. Possible vapC23,toxin, part of toxin-antitoxin (TA) operon with Rv2862A,contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Q50595|YI38_MYCTU|Rv1838c|MT1886|MTCY1A11.05|MTCY359.35 conserved hypothetical protein (131 aa), FASTA scores: opt: 299, E(): 6.5e-15, (39.0% identity in 123 aa overlap).; Possible toxin VapC23 3175372 vapC23 888195 vapC23 Mycobacterium tuberculosis H37Rv Possible toxin VapC23 NP_217379.1 3174992 D 83332 CDS NP_217380.1 15610001 888496 complement(3175454..3177265) 1 NC_000962.3 Rv2864c, (MTV003.10c), len: 603 aa. Possible penicillin-binding lipoprotein, probably located in periplasm, equivalent to Q9CBU6|ML1577 probable penicillin binding protein from Mycobacterium leprae (608 aa), FASTA scores: opt: 3352, E(): 2.1e-193, (81.5% identity in 606 aa overlap). Also shows some similarity to others e.g. P72405|PCBR from Streptomyces clavuligerus (551 aa), FASTA scores: opt: 543, E(): 6.1e-25, (28.4% identity in 567 aa overlap); Q9F2L0|SCH63.18c from Streptomyces coelicolor (546 aa), FASTA scores: opt: 519, E(): 1.7e-23, (29.3% identity in 577 aa overlap); Q9RKD1|SCE87.07 from Streptomyces coelicolor (541 aa), FASTA scores: opt: 472,E(): 1.1e-20, (34.3% identity in 318 aa overlap); etc. Equivalent to AAK47258 from Mycobacterium tuberculosis strain CDC1551 (618 aa) but shorter 15 aa. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and PS00017 ATP/GTP-binding site motif A (P-loop).; Possible penicillin-binding lipoprotein 3177265 888496 Rv2864c Mycobacterium tuberculosis H37Rv Possible penicillin-binding lipoprotein NP_217380.1 3175454 R 83332 CDS NP_217381.1 15610002 887458 3177537..3177818 1 NC_000962.3 Rv2865, (MTV003.11), len: 93 aa. RelF, antitoxin,part of toxin-antitoxin (TA) operon with Rv2866 (See Pandey and Gerdes, 2005), showing weak similarity with P58235|YR54_SYNY3|SSR2754 hypothetical 9.7 KDA protein from Synechocystis sp. strain PCC 6803 (87 aa), FASTA scores: opt: 134, E(): 0.007, (30.65% identity in 75 aa overlap); BAB58570|SAV2408 conserved hypothetical protein from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 124, E(): 0.037, (27.5% identity in 80 aa overlap). Also similar to Rv1247|MTV006.19c hypothetical 9.8 KDA protein from Mycobacterium tuberculosis (89 aa),FASTA scores: opt: 249, E(): 2.6e-11, (44.2% identity in 86 aa overlap).; Antitoxin RelF 3177818 relF 887458 relF Mycobacterium tuberculosis H37Rv Antitoxin RelF NP_217381.1 3177537 D 83332 CDS NP_217382.1 15610003 887450 3177822..3178085 1 NC_000962.3 Rv2866, (MTV003.12), len: 87 aa. RelG, toxin, part of toxin-antitoxin (TA) operon with Rv2865 (See Pandey and Gerdes, 2005), similar to O50461|Rv1246c|MTV006.18c conserved hypothetical protein from Mycobacterium tuberculosis (97 aa), FASTA scores: opt: 290, E(): 3.6e-16,(54.1% identity in 85 aa overlap).; Toxin RelG 3178085 relG 887450 relG Mycobacterium tuberculosis H37Rv Toxin RelG NP_217382.1 3177822 D 83332 CDS NP_217383.1 15610004 887475 complement(3178458..3179312) 1 NC_000962.3 Rv2867c, (MTV003.13c), len: 284 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Similar to others e.g. Q9KYR8|SC5H4.21 hypothetical 31.3 KDA protein from Streptomyces coelicolor (287 aa), FASTA scores: opt: 798, E(): 2.4e-45, (47.95% identity in 269 aa overlap).; GCN5-related N-acetyltransferase 3179312 887475 Rv2867c Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_217383.1 3178458 R 83332 CDS NP_217384.1 15610005 887463 complement(3179368..3180531) 1 NC_000962.3 Rv2868c, (MTV003.14c), len: 387 aa. Probable gcpE protein (protein e), equivalent to Q9CBU5|GCPE|ML1581 hypothetical protein GCPE from Mycobacterium leprae (392 aa), FASTA scores: opt: 2247, E(): 6.8e-134, (87.65% identity in 388 aa overlap). Highly similar to essential gene of unknown function from Escherichia coli and other prokaryotes e.g. Q9X7W2|GCPE_STRCO|SC6A5.16 GCPE protein homolog from Streptomyces coelicolor (384 aa), FASTA scores: opt: 1965, E(): 3.8e-116, (78.2% identity in 385 aa overlap); P54482|GCPE_BACSU GCPE protein homolog from Bacillus subtilis (377 aa), FASTA scores: opt: 1157, E(): 2.6e-65, (49.55% identity in 351 aa overlap); P27433|GCPE_ECOLI|B2515|Z3778|ECS3377 GCPE protein (protein E) from Escherichia coli strains K12 and O157:H7 (372 aa),FASTA scores: opt: 984, E(): 2e-54, (44.15% identity in 360 aa overlap); etc. Belongs to the GCPE family.; Probable GcpE protein 3180531 gcpE 887463 gcpE Mycobacterium tuberculosis H37Rv Probable GcpE protein NP_217384.1 3179368 R 83332 CDS NP_217385.1 15610006 887449 complement(3180548..3181762) 1 NC_000962.3 Rv2869c, (MTV003.15c), len: 404 aa. Rip,metalloprotease, regulates intramembrane proteolysis and controls membrane composition (rip, see Makinoshima and Glickman, 2005). Similar to site two protease (S2P) in higher eukaryotes. Conserved transmembrane protein,equivalent to Q9CBU4|ML1582 probable integral membrane protein from Mycobacterium leprae (404 aa), FASTA scores: opt: 2250, E(): 1.1e-128, (82.2% identity in 404 aa overlap). Also weakly similar to other membrane proteins or hypothetical proteins e.g. Q9A710|CC1916 putative membrane-associated zinc metalloprotease from Caulobacter crescentus (398 aa), FASTA scores: opt: 368, E(): 7.8e-15,(28.1% identity in 427 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Cleaves PbpB|Rv2163c in a Zn2+ -dependent manner (See Mukherjee et al., 2009). Cleaves proteins RskA|Rv0444c, RslA|Rv0736, and Rv3912, in M. tuberculosis Erdman (See Sklar et al., 2010).; Membrane bound metalloprotease 3181762 rip 887449 rip Mycobacterium tuberculosis H37Rv Membrane bound metalloprotease NP_217385.1 3180548 R 83332 CDS NP_217386.2 57117032 887800 complement(3181770..3183011) 1 NC_000962.3 Rv2870c, (MTCY274.01c, MTV003.16c), len: 413 aa. Probable dxr, 1-deoxy-D-xylulose 5-phosphate reductoisomerase, equivalent to Q9CBU3|DXR|ML1583 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Mycobacterium leprae (406 aa), FASTA scores: opt: 2145,E(): 1e-124, (84.05% identity in 395 aa overlap). Also highly similar to others e.g. Q9AJD7|DXR from Kitasatospora griseola (Streptomyces griseolosporeus) (386 aa), FASTA scores: opt: 1176, E(): 5.2e-65, (56.45% identity in 388 aa overlap); Q9KYS1|DXR_STRCO|SC5H4.18 from Streptomyces coelicolor (401 aa), FASTA scores: opt: 1079, E(): 5.1e-59,(52.25% identity in 396 aa overlap); P45568|DXR|B0173 from Escherichia coli strain K12 (398 aa), FASTA scores: opt: 120, E(): 0.032, (52.9% identity in 34 aa overlap); etc. Contains PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature. Belongs to the DXR family. N-terminus shortened since first submission.; Probable 1-deoxy-D-xylulose 5-phosphate reductoisomerase Dxr (DXP reductoisomerase) (1-deoxyxylulose-5-phosphate reductoisomerase) 3183011 dxr 887800 dxr Mycobacterium tuberculosis H37Rv Probable 1-deoxy-D-xylulose 5-phosphate reductoisomerase Dxr (DXP reductoisomerase) (1-deoxyxylulose-5-phosphate reductoisomerase) NP_217386.2 3181770 R 83332 CDS NP_217387.1 15610008 887468 3183138..3183395 1 NC_000962.3 Rv2871, (MTCY274.02), len: 85 aa. Possible vapB43,antitoxin, part of toxin-antitoxin (TA) operon with Rv2872,see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O50456|Rv1241|MTV006.13 (86 aa), FASTA scores: opt: 172,E(): 2.9e-05, (37.2% identity in 86 aa overlap); O53811|Rv0748|MTV041.22 (85 aa), FASTA scores: opt: 170,E(): 4e-05, (35.3% identity in 85 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible antitoxin VapB43 3183395 vapB43 887468 vapB43 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB43 NP_217387.1 3183138 D 83332 CDS NP_217388.1 15610009 887361 3183382..3183825 1 NC_000962.3 Rv2872, (MTCY274.03), len: 147 aa. Possible vapC43,toxin, part of toxin-antitoxin (TA) operon with Rv2871,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O53683|Rv0277c|MTV035.05c (142 aa), FASTA scores: opt: 357, E(): 1.4e-17, (41.45% identity in 140 aa overlap); O53812|Rv0749|MTV041.23 (142 aa), FASTA scores: opt: 350, E(): 4.3e-17, (41.55% identity in 142 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible toxin VapC43 Contains PIN domain 3183825 vapC43 887361 vapC43 Mycobacterium tuberculosis H37Rv Possible toxin VapC43 Contains PIN domain NP_217388.1 3183382 D 83332 CDS NP_217389.1 15610010 887155 3183905..3184567 1 NC_000962.3 Rv2873, (MTCY274.04), len: 220 aa. Mpt83 (alternate gene name: mpb83), cell surface lipoprotein (see citations below). Also similar to upstream ORF Q50769|MP70_MYCTU|MPT70|MPB70|Rv2875|MT2943|MTCY274.06 which is also known as major secreted immunogenic protein MPT70 precursor from Mycobacterium tuberculosis (193 aa),FASTA scores: opt: 806, E(): 2.7e-38, (70.25% identity in 185 aa overlap). Belongs to the MPT70 / MPT83 family. Attached to the membrane by a lipid anchor.; Cell surface lipoprotein Mpt83 (lipoprotein P23) 3184567 mpt83 887155 mpt83 Mycobacterium tuberculosis H37Rv Cell surface lipoprotein Mpt83 (lipoprotein P23) NP_217389.1 3183905 D 83332 CDS NP_217390.1 15610011 888162 3184847..3186934 1 NC_000962.3 Rv2874, (MT2942, MTCY274.05), len: 695 aa. Possible dipZ, cytochrome c-type biogenesis protein (see citation below), probable integral membrane protein, similar in part to others or hypothetical proteins e.g. CAC48606|SMB20213 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (627 aa), FASTA scores: opt: 844,E(): 7.3e-43, (32.65% identity in 643 aa overlap); Q9ZMH0|CCDA or JHP0250 putative cytochrome C-type biogenesis protein from Helicobacter pylori J99 (Campylobacter pylori J99) (239 aa), FASTA scores: opt: 250, E(): 1.4e-07, (27.3% identity in 227 aa overlap); Q9LA04|CCDA C-type cytochrome biogenesis protein from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (252 aa), FASTA scores: opt: 245, E(): 2.9e-07, (27.85% identity in 244 aa overlap); etc. Also similar to O06393|CCSA|Rv0527|MTCY25D10.06 cytochrome C-type biogenesis protein from Mycobacterium tuberculosis (259 aa), FASTA scores: opt: 280, E(): 2.4e-09, (29.3% identity in 239 aa overlap).; Possible integral membrane C-type cytochrome biogenesis protein DipZ 3186934 dipZ 888162 dipZ Mycobacterium tuberculosis H37Rv Possible integral membrane C-type cytochrome biogenesis protein DipZ NP_217390.1 3184847 D 83332 CDS NP_217391.1 15610012 887724 3187030..3187611 1 NC_000962.3 Rv2875, (MTCY274.06), len: 193 aa. Mpt70 (alternate gene name: mpb70), major secreted immunogenic protein MPT70 precursor (see citations below). Also similar to downstream ORF Q10790|MP83_MYCTU|MPT83|MPB83|Rv2873|MT2940|MTCY274.04 cell surface lipoprotein MPT83 precursor (lipoprotein P23) (220 aa), FASTA scores: opt: 806, E(): 1.2e-40, (70.25% identity in 185 aa overlap). Belongs to the MPT70 / MPT83 family. Generally found as a monomer; homodimer in culture fluids.; Major secreted immunogenic protein Mpt70 3187611 mpt70 887724 mpt70 Mycobacterium tuberculosis H37Rv Major secreted immunogenic protein Mpt70 NP_217391.1 3187030 D 83332 CDS NP_217392.1 15610013 887823 3187663..3187977 1 NC_000962.3 Rv2876, (MTCY274.07), len: 104 aa. Possible conserved transmembrane protein, equivalent (but longer 16 aa) to Q9CBU2|ML1584 possible conserved membrane protein from Mycobacterium leprae (84 aa), FASTA scores: opt: 444,E(): 8.3e-26, (73.85% identity in 88 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved transmembrane protein 3187977 887823 Rv2876 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_217392.1 3187663 D 83332 CDS YP_177912.1 57117033 887196 complement(3188008..3188871) 1 NC_000962.3 Rv2877c, (MTCY274.08c), len: 287 aa. Probable conserved integral membrane protein, Mer family possibly involved in transport of mercury, similar to others, and to the fourth protein of the mercury resistance operon of Streptomyces sp (or other organisms), and to putative cytochrome-c biogenesis proteins e.g. Q9XBD1|CZA382.20C putative integral membrane transporter from Amycolatopsis orientalis (298 aa), FASTA scores: opt: 913, E(): 7.6e-46,(51.55% identity in 293 aa overlap); P30344|MER4_STRLI mercury resistance probable HG transport protein from Streptomyces lividans (319 aa), FASTA scores: opt: 427,E(): 1.2e-17, (32.85% identity in 289 aa overlap); Q9M5P3 putative cytochrome C biogenesis protein precursor from Arabidopsis thaliana (Mouse-ear cress) (354 aa), FASTA scores: opt: 229, E(): 4e-06, (29.85% identity in 221 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature. Note that previously known as merT.; Probable conserved integral membrane protein 3188871 887196 Rv2877c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein YP_177912.1 3188008 R 83332 CDS NP_217394.1 15610015 887184 complement(3188876..3189397) 1 NC_000962.3 Rv2878c, (MT2946, MTCY274.09c), len: 173 aa. Mpt53,secreted protein (contains N-terminal signal sequence) (see citations below). Shows some similarity with several disulfide bond interchange proteins e.g. P43787|THIX_HAEIN thioredoxin-like protein HI1115 from Haemophilus influenzae (167 aa), FASTA scores: opt: 200, E(): 1.4e-06, (28.9% identity in 135 aa overlap); P52237|TIPB_PSEFL thiol:disulfide interchange protein TIPB precursor (cytochrome C biogenesis protein TIPB) (178 aa), FASTA scores: opt: 184, E(): 1.8e-05, (26.3% identity in 171 aa overlap); etc. Also highly similar to O53924|DSBF|Rv1677|MTV047.12 putative lipoprotein from Mycobacterium tuberculosis (182 aa), FASTA scores: opt: 482, E(): 5.7e-26, (52.8% identity in 142 aa overlap). Could be belong to the thioredoxin family. Note that also previously known as dsbE.; Soluble secreted antigen Mpt53 precursor 3189397 mpt53 887184 mpt53 Mycobacterium tuberculosis H37Rv Soluble secreted antigen Mpt53 precursor NP_217394.1 3188876 R 83332 CDS NP_217395.1 15610016 887156 complement(3189583..>3190152) 1 NC_000962.3 Rv2879c, (MTCY274.10c), len: 189 aa. Conserved hypothetical protein, similar to others e.g. C-terminus of Q9RVT6|DR0936 conserved hypothetical protein from Deinococcus radiodurans (346 aa), FASTA scores: opt: 505,E(): 1e-26, (46.5% identity in 185 aa overlap); O34617|YLON_BACSU hypothetical 41.6 KDA protein from Bacillus subtilis (363 aa), FASTA scores: opt: 459, E(): 1.2e-24, (40.5% identity in 185 aa overlap); YFGB_ECOLI|P36979 hypothetical 43.1 kDa protein from Escherichia coli (384 aa), FASTA scores, opt: 410, E(): 2.8e-21, (41.7% identity in 187 aa overlap); etc. Appears to be a frame shift with respect to following ORF but we can detect no error in the cosmid sequence to account for this.; hypothetical protein >3190152 887156 Rv2879c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217395.1 3189583 R 83332 CDS NP_217396.1 15610017 887810 complement(3189851..3190678) 1 NC_000962.3 Rv2880c, (MTCY274.11c), len: 275 aa. Conserved hypothetical protein, highly similar in N-terminus to others e.g. O86754|SC6A9.22c hypothetical 40.4 KDA protein from Streptomyces coelicolor (368 aa), FASTA scores: opt: 663, E(): 2.6e-33, (52.6% identity in 213 aa overlap); Q55880|Y098_SYNY3|SLL0098 hypothetical 38.9 KDA protein from Synechocystis sp. strain PCC 6803 (350 aa), FASTA scores: opt: 362, E(): 7.3e-15, (38.9% identity in 162 aa overlap); O66732|AQ_416 hypothetical 40.2 KDA protein from Aquifex aeolicus (348 aa), FASTA scores: opt: 321, E(): 2.4e-12, (39.75% identity in 146 aa overlap); etc. Appears to be a frame shift with respect to preceding ORF but we can detect no error in the cosmid sequence to account for this.; hypothetical protein 3190678 887810 Rv2880c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217396.1 3189851 R 83332 CDS NP_217397.1 15610018 888910 complement(3190701..3191621) 1 NC_000962.3 Rv2881c, (MTCY274.12c), len: 306 aa. Probable cdsA,phosphatidate cytidylyltransferase, integral membrane protein, equivalent to Q9CBU1|CDSA_MYCLE|ML1589 phosphatidate cytidylyltransferase from Mycobacterium leprae (312 aa), FASTA scores: opt: 1470, E(): 1.1e-84,(70.3% identity in 313 aa overlap). Also similar to others e.g. Q9KPV7|VC2255 from Vibrio cholerae (280 aa), FASTA scores: opt: 383, E(): 1.1e-16, (29.3% identity in 280 aa overlap); Q9CDT2|CDSA from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (267 aa), FASTA scores: opt: 361, E(): 2.6e-15, (29.05% identity in 265 aa overlap); P06466|CDSA_ECOLI|CDS|B0175|Z0186|ECS0177 from Escherichia coli strains K12 and O157:H7 (249 aa), FASTA scores: opt: 352, E(): 9.2e-15, (40.4% identity in 156 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CDS family.; Probable integral membrane phosphatidate cytidylyltransferase CdsA (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase) 3191621 cdsA 888910 cdsA Mycobacterium tuberculosis H37Rv Probable integral membrane phosphatidate cytidylyltransferase CdsA (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase) NP_217397.1 3190701 R 83332 CDS NP_217398.1 15610019 887464 complement(3191644..3192201) 1 NC_000962.3 Rv2882c, (MTCY274.13c), len: 185 aa. Probable frr,ribosome recycling factor, equivalent to O33046|RRF_MYCLE|FRR|ML1590|MLCB250.76 ribosome recycling factor from Mycobacterium leprae (185 aa), FASTA scores: opt: 1063, E(): 2.6e-60, (90.8% identity in 185 aa overlap). Also highly similar to others e.g. O86770|RRF_STRCO|FRR|SC6A9.40c from Streptomyces coelicolor (185 aa), FASTA scores: opt: 783, E(): 1.5e-42, (63.25% identity in 185 aa overlap); P81101|RRF_BACSU|FRR from Bacillus subtilis (184 aa), FASTA scores: opt: 640, E(): 1.7e-33, (51.65% identity in 182 aa overlap); P16174|RRF_ECOLI|FRR|B0172|Z0183|ECS0174 from Escherichia coli strains K12 and O157:H7 (185 aa), FASTA scores: opt: 473, E(): 1.4e-23, (40.2% identity in 184 aa overlap); etc. Belongs to the RRF family.; Ribosome recycling factor Frr (ribosome releasing factor) (RRF) 3192201 frr 887464 frr Mycobacterium tuberculosis H37Rv Ribosome recycling factor Frr (ribosome releasing factor) (RRF) NP_217398.1 3191644 R 83332 CDS NP_217399.1 15610020 887709 complement(3192373..3193158) 1 NC_000962.3 Rv2883c, (MT2951, MTCY274.14c), len: 261 aa. Probable pyrH, uridylate kinase, equivalent to O33045|PYRH_MYCLE|ML1591|MLCB250.75 uridylate kinase from Mycobacterium leprae (279 aa), FASTA scores: opt: 1437,E(): 3.8e-81, (85.05% identity in 274 aa overlap). Also highly similar to others e.g. O69913|PYRH from Streptomyces coelicolor (253 aa), FASTA scores: opt: 1086, E(): 1.4e-59,(68.9% identity in 251 aa overlap); P74457|PYRH_SYNY3|SLL0144 from Synechocystis sp. strain PCC 6803 (260 aa), FASTA scores: opt: 851, E(): 4.1e-45,(55.85% identity in 231 aa overlap); P29464|PYRH_ECOLI|SMBA|B0171|Z0182|ECS0173 from strains K12 and O157:H7 (240 aa), FASTA scores: opt: 666, E(): 1.1e-35,(45.7% identity in 232 aa overlap); etc.; Probable uridylate kinase PyrH (UK) (uridine monophosphate kinase) (UMP kinase) 3193158 pyrH 887709 pyrH Mycobacterium tuberculosis H37Rv Probable uridylate kinase PyrH (UK) (uridine monophosphate kinase) (UMP kinase) NP_217399.1 3192373 R 83332 CDS NP_217400.1 15610021 887443 3193393..3194151 1 NC_000962.3 Rv2884, (MTCY274.15), len: 252 aa. Probable transcriptional regulatory protein, highly similar to others e.g. Q05943|GLNR_STRCO|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa),FASTA scores: opt: 609, E(): 2.7e-34, (46.4% identity in 224 aa overlap); Q55733|SLL0396 regulatory components of sensory transduction system from Synechocystis sp. strain PCC 6803 (224 aa), FASTA scores: opt: 330, E(): 3e-15,(31.8% identity in 217 aa overlap); Q9A4S3|CC2757 DNA-binding response regulator from Caulobacter crescentus (223 aa), FASTA scores: opt: 311, E(): 6e-14, (30.3% identity in 221 aa overlap); etc. Also highly similar to O53830|Rv0818|MTV043.10 putative regulatory protein from Mycobacterium tuberculosis (255 aa), FASTA scores: opt: 665, E(): 3.8e-38, (47.6% identity in 227 aa overlap). The N-terminal region is similar to that of other regulatory components of sensory transduction systems.; Probable transcriptional regulatory protein 3194151 887443 Rv2884 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_217400.1 3193393 D 83332 CDS NP_217401.1 15610022 887173 complement(3194166..3195548) 1 NC_000962.3 Rv2885c, (MTCY274.16c), len: 460 aa. Probable transposase for IS1539. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable transposase 3195548 887173 Rv2885c Mycobacterium tuberculosis H37Rv Probable transposase NP_217401.1 3194166 R 83332 CDS NP_217402.1 15610023 887424 complement(3195545..3196432) 1 NC_000962.3 Rv2886c, (MTCY274.17c), len: 295 aa. Probable resolvase for IS1539. Contains PS00213 Lipocalin signature.; Probable resolvase 3196432 887424 Rv2886c Mycobacterium tuberculosis H37Rv Probable resolvase NP_217402.1 3195545 R 83332 CDS NP_217403.1 15610024 888563 3196431..3196850 1 NC_000962.3 Rv2887, (MTCY274.18), len: 139 aa. Probable transcriptional regulatory protein, highly similar to Q9EX59|SC1A4.04 putative MarR-family transcriptional regulator from Streptomyces coelicolor (151 aa), FASTA scores: opt: 354, E(): 6.6e-16, (42.95% identity in 135 aa overlap); and similar to others e.g. AAF97817|SLYA transcriptional regulator SLYA from Escherichia coli strain EPEC 2348/69 (146 aa), FASTA scores: opt: 181, E(): 0.0001,(27.25% identity in 132 aa overlap); P55740|SLYA_ECOLI|AAG56631|B1642|Z2657|ECS2351 transcriptional regulator SLYA from Escherichia coli strains K12 and O157:H7 (146 aa), FASTA scores: opt: 177,E(): 0.00018, (27.25% identity in 132 aa overlap) ; etc. Contains probable helix-turn-helix motif at aa 50-71 (Score 1182, +3.21 SD).; Probable transcriptional regulatory protein 3196850 888563 Rv2887 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_217403.1 3196431 D 83332 CDS NP_217404.1 15610025 887401 complement(3196864..3198285) 1 NC_000962.3 Rv2888c, (MTCY274.19c), len: 473 aa. Probable amiC,amidase, equivalent to O33040|AMI3_MYCLE|AMIC|ML1596|MLCB250.65 putative amidase AMIC from Mycobacterium leprae (468 aa), FASTA scores: opt: 2361, E(): 4.2e-139, (76.7% identity in 468 aa overlap). Also similar to others e.g. Q9A8N0|CC1323 putative 6-aminohexanoate-cyclic-dimer hydrolase from Caulobacter crescentus (521 aa), FASTA scores: opt: 925, E(): 7.4e-50,(36.55% identity in 465 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 659,E(): 2.2e-33, (31.1% identity in 460 aa overlap); Q55424|AMID_SYNY3|SLL0828 putative amidase from Synechocystis sp. strain PCC 6803 (506 aa), FASTA scores: opt: 643, E(): 2.4e-32, (30.7% identity in 466 aa overlap); etc. Also similar to O05835|AMI1_MYCTU|AMIA2|Rv2363|MT2432|MTCY27.17c putative amidase AMIA2 (484 aa), FASTA scores: opt: 656, E(): 3.6e-33, (35.9% identity in 465 aa overlap); and Q11056|AMI2_MYCTU|AMIB2|Rv1263|MT1301|MTCY50.19c putative amidase from Mycobacterium tuberculosis (462 aa), FASTA scores: opt: 650, E(): 8.2e-33, (33.45% identity in 472 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-poop). Belongs to the amidase family.; Probable amidase AmiC (aminohydrolase) 3198285 amiC 887401 amiC Mycobacterium tuberculosis H37Rv Probable amidase AmiC (aminohydrolase) NP_217404.1 3196864 R 83332 CDS NP_217405.1 15610026 888187 complement(3198292..3199107) 1 NC_000962.3 Rv2889c, (MTCY274.20c), len: 271 aa. Probable tsf,elongation factor, equivalent to O33039|EFTS_MYCLE|TSF|ML1597|MLCB250.64 elongation factor from Mycobacterium leprae (276 aa), FASTA scores: opt: 1430, E(): 1.9e-80, (83.7% identity in 276 aa overlap). Also highly similar to others e.g. Q9X5Z9|EFTS_STRRA|TSF from Streptomyces ramocissimus (278 aa), FASTA scores: opt: 928, E(): 1.1e-49, (57.05% identity in 277 aa overlap); O31213|EFTS_STRCO|TSF|SC2E1.42 from Streptomyces coelicolor (278 aa), FASTA scores: opt: 927, E(): 1.3e-49, (56.3% identity in 277 aa overlap); P80700|EFTS_BACSU|TSF from Bacillus subtilis (292 aa), FASTA scores: opt: 650, E(): 1.3e-32, (43.85% identity in 276 aa overlap); etc. Contains PS01127 Elongation factor Ts signature 2. Belongs to the EF-ts family.; Probable elongation factor Tsf (EF-ts) 3199107 tsf 888187 tsf Mycobacterium tuberculosis H37Rv Probable elongation factor Tsf (EF-ts) NP_217405.1 3198292 R 83332 CDS NP_217406.1 15610027 887187 complement(3199119..3199982) 1 NC_000962.3 Rv2890c, (MTCY274.21c), len: 287 aa. rpsB, 30s ribosomal protein s2, equivalent to O33038|RS2_MYCLE|RPSB|ML1598|MLCB250.63 30S ribosomal protein S2 from Mycobacterium leprae (277 aa), FASTA scores: opt: 1593, E(): 2.3e-93, (91.5% identity in 270 aa overlap). Also highly similar to others e.g. O31212|RS2_STRCO|RPSB|SC2E1.41 from Streptomyces coelicolor (310 aa), FASTA scores: opt: 1302, E(): 6.1e-75, (70.6% identity in 289 aa overlap); Q9KA63|RPSB|BH2427 from Bacillus halodurans (244 aa), FASTA scores: opt: 991, E(): 2.3e-55, (59.6% identity in 255 aa overlap); P21464|RS2_BACSU|RPSB from Bacillus subtilis (245 aa),FASTA scores: opt: 959, E(): 2.4e-53, (58.55% identity in 246 aa overlap); etc. Contains PS00962 Ribosomal protein S2 signature 1. Belongs to the S2P family of ribosomal proteins.; 30S ribosomal protein S2 RpsB 3199982 rpsB 887187 rpsB Mycobacterium tuberculosis H37Rv 30S ribosomal protein S2 RpsB NP_217406.1 3199119 R 83332 CDS NP_217407.1 15610028 888328 3200266..3201015 1 NC_000962.3 Rv2891, (MTCY274.22), len: 249 aa (C-terminus overlaps neigbouring ORF). Conserved hypothetical protein,similar in N-terminus to O69910|SC2E1.40c hypothetical 22.8 KDA protein from Streptomyces coelicolor (226 aa), FASTA scores: opt: 315, E(): 3.4e-11, (40.7% identity in 145 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 3201015 888328 Rv2891 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217407.1 3200266 D 83332 CDS YP_177913.1 57117034 887824 complement(3200794..3202020) 1 NC_000962.3 Rv2892c, (MTCY274.23c), len: 408 aa. PPE45, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O06386|Rv3621c|MTCY15C10.31|MTCY07H7B.01 from M. tuberculosis (413 aa), FASTA scores: opt: 957, E(): 6.2e-46, (44.7% identity in 423 aa overlap).; PPE family protein PPE45 3202020 PPE45 887824 PPE45 Mycobacterium tuberculosis H37Rv PPE family protein PPE45 YP_177913.1 3200794 R 83332 CDS NP_217409.1 15610030 887337 3202420..3203397 1 NC_000962.3 Rv2893, (MTCY274.24), len: 325 aa. Possible oxidoreductase, showing similarity with various proteins and/or oxidoreductases e.g. Q9AE05|RIF11 eleventh protein in the rif biosynthetic gene cluster from Amycolatopsis mediterranei (Nocardia mediterranei) (294 aa), FASTA scores: opt: 270, E(): 4.8e-10, (34.5% identity in 313 aa overlap); O52567 reductase from Amycolatopsis mediterranei (Nocardia mediterranei) (153 aa), FASTA scores: opt: 251,E(): 5e-09, (42.4% identity in 125 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 249,E(): 1.2e-08, (29.7% identity in 283 aa overlap); etc. Also some similarity with others proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. P71844|Rv0791c|MTCY369.35c putative oxidoreductase (347 aa), FASTA scores: opt: 264, E(): 1.3e-09, (29.05% identity in 272 aa overlap); and P96809|Rv0132|MTCI5.06c putative oxidoreductase (360 aa), FASTA scores: opt: 260, E(): 2.4e-09, (33.05% identity in 239 aa overlap).; Possible oxidoreductase 3203397 887337 Rv2893 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_217409.1 3202420 D 83332 CDS NP_217410.1 15610031 887392 complement(3203394..3204290) 1 NC_000962.3 Rv2894c, (MTCY274.25c), len: 298 aa. Probable xerC,integrase/recombinase, equivalent to Q9CBU0|XERC|ML1600|MLCB250.62 integrase/recombinase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1624,E(): 2e-97, (85.15% identity in 296 aa overlap). Also highly similar to others integrases/recombinases (generally xerC and xerD) e.g. Q9HTS4|SSS|PA5280 site-specific recombinase from Pseudomonas aeruginosa (303 aa), FASTA scores: opt: 660, E(): 3.2e-35, (41.8% identity in 299 aa overlap); Q9HXQ6|XERD|PA3738 integrase/recombinase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 656,E(): 5.7e-35, (40.05% identity in 297 aa overlap); Q9KCP0|BH1529 integrase/recombinase from Bacillus halodurans (299 aa), FASTA scores: opt: 645, E(): 2.9e-34,(37.35% identity in 300 aa overlap); etc. Also similar to O33200|Rv1701|MTCI125.23 integrase/recombinase from Mycobacterium tuberculosis (311 aa), FASTA scores: opt: 646, E(): 2.6e-34, (43.1% identity in 304 aa overlap). Belongs to the 'phage' integrase family.; Probable integrase/recombinase XerC 3204290 xerC 887392 xerC Mycobacterium tuberculosis H37Rv Probable integrase/recombinase XerC NP_217410.1 3203394 R 83332 CDS NP_217411.1 15610032 888180 complement(3204381..3205232) 1 NC_000962.3 Rv2895c, (MT2963, MTCY274.26c), len: 283 aa. Possible viuB, mycobactin utilization protein, highly similar to Q9RJ78|SCI41.06 hypothetical 31.5 KDA protein from Streptomyces coelicolor (280 aa), FASTA scores: opt: 639, E(): 5.1e-32, (46.3% identity in 285 aa overlap); and similar to other proteins e.g. Q9F641|MXCB protein of the biosynthetic gene cluster of the myxochelin-type iron chelator from Stigmatella aurantiaca (270 aa), FASTA scores: opt: 417, E(): 2.2e-18, (34.2% identity in 263 aa overlap); Q56646|VIUB_VIBCH|VC2210 vibriobactin utilization protein from Vibrio cholerae (271 aa), FASTA scores: opt: 395, E(): 5.1e-17, (31.0% identity in 274 aa overlap); Q56743|VIUB_VIBVU vulnibactin utilization protein V from Vibrio vulnificus (271 aa), FASTA scores: opt: 390, E(): 1e-16, (33.95% identity in 274 aa overlap); etc. Equivalent to AAK47289 from Mycobacterium tuberculosis strain CDC1551 (321 aa) but shorter 38 aa.; Possible mycobactin utilization protein ViuB 3205232 viuB 888180 viuB Mycobacterium tuberculosis H37Rv Possible mycobactin utilization protein ViuB NP_217411.1 3204381 R 83332 CDS NP_217412.2 448824793 887177 complement(3205265..3206434) 1 NC_000962.3 Rv2896c, (MTCY274.27c), len: 389 aa. Conserved hypothetical protein, similar to others proteins e.g. Q9ZJ08|FIR2 from Rhodococcus fascians (293 aa), FASTA scores: opt: 663, E(): 3.3e-32, (43.7% identity in 286 aa overlap); O69892|SC2E1.21 hypothetical 37.9 KDA protein from Streptomyces coelicolor (382 aa), FASTA scores: opt: 600, E(): 2.2e-28, (46.45% identity in 267 aa overlap); Q9JWZ4|DPRA|NMA0158 DPRA homolog from Neisseria meningitidis (serogroup A) (395 aa), FASTA scores: opt: 495, E(): 4.1e-22, (34.6% identity in 347 aa overlap); etc. Nucleotide position 3205978 in the genome sequence has been corrected, A:C resulting in S153A.; hypothetical protein 3206434 887177 Rv2896c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217412.2 3205265 R 83332 CDS NP_217413.1 15610034 888212 complement(3206431..3207942) 1 NC_000962.3 Rv2897c, (MTCY274.28c), len: 503 aa. Conserved hypothetical protein, possibly Mg-chelatase, highly similar to hypothetical proteins and chelatases e.g. Q9RTV0|DR1656 mg(2+) chelatase family protein from Deinococcus radiodurans (519 aa), FASTA scores: opt: 1333, E(): 3.6e-68, (46.55% identity in 505 aa overlap);Q55372|SLR0904 hypothetical 55.1 KDA protein from Synechocystis sp. strain PCC 6803 (509 aa), FASTA scores: opt: 1271, E(): 1.2e-64,(42.65% identity in 504 aa overlap); Q9HTR4|PA5290 hypothetical protein from Pseudomonas aeruginosa (497 aa),FASTA scores: opt: 1248, E(): 2.3e-63, (45.9% identity in 503 aa overlap); Q9K0Z6|comm|NMB0405 competence protein (mg-chelatase) from Neisseria meningitidis (serogroup B),FASTA scores: opt: 1229, E(): 2.8e-62, (43.2% identity in 509 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; hypothetical protein 3207942 888212 Rv2897c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217413.1 3206431 R 83332 CDS NP_217414.1 15610035 888507 complement(3207942..3208328) 1 NC_000962.3 Rv2898c, (MTCY274.29c), len: 128 aa. Conserved hypothetical protein, highly similar to O33024|YS98_MYCLE|ML1607|MLCB250.49 hypothetical 11.0 KDA protein from Mycobacterium leprae (96 aa), FASTA scores: opt: 318, E(): 2.3e-16, (58.35% identity in 96 aa overlap). Also similar to other hypothetical proteins e.g. O69890|YE19_STRCO|SC2E1.19 from Streptomyces coelicolor (130 aa), FASTA scores: opt: 253, E(): 1.7e-11, (39.65% identity in 121 aa overlap); Q9HVZ1|PA4424 from Pseudomonas aeruginosa (125 aa), FASTA scores: opt: 234, E(): 4.2e-10,(40.85% identity in 115 aa overlap); O86871 from Streptomyces lividans (85 aa), FASTA scores: opt: 224, E(): 1.8e-09, (46.45% identity in 84 aa overlap); etc. Equivalent to AAK47292 from Mycobacterium tuberculosis strain CDC1551 (141 aa) but shorter 13 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3208328 888507 Rv2898c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217414.1 3207942 R 83332 CDS NP_217415.1 15610036 888201 complement(3208576..3209406) 1 NC_000962.3 Rv2899c, (MTCY274.30c), len: 276 aa. Possible fdhD protein homolog, highly similar to other bacterial fdhd protein homologs or formate dehydrogenase accessory proteins e.g. Q9ZBW0|FDHD_STRCO|SC4B5.08c from Streptomyces coelicolor (282 aa), FASTA scores: opt: 1032, E(): 3.6e-59,(59.0% identity in 278 aa overlap); BAB59387|TVG0258796 from Thermoplasma volcanium (279 aa), FASTA scores: opt: 536, E(): 3.4e-27, (38.65% identity in 282 aa overlap); Q9HL17|FDHD_THEAC|TA0423 from Thermoplasma acidophilum (282 aa), FASTA scores: opt: 529, E(): 9.6e-27, (38.8% identity in 281 aa overlap); P32177|FDHD_ECOLI FDHD protein from Escherichia coli strain K12 (277 aa), FASTA scores: opt: 297, E(): 8.6e-12, (33.35% identity in 261 aa overlap); etc. Contain a Pfam match to entry PF02634 FdhD/NarQ family. Belongs to the FdhD family.; Possible FdhD protein homolog 3209406 fdhD 888201 fdhD Mycobacterium tuberculosis H37Rv Possible FdhD protein homolog NP_217415.1 3208576 R 83332 CDS NP_217416.1 15610037 887987 complement(3209406..3211745) 1 NC_000962.3 Rv2900c, (MTCY274.31c), len: 779 aa. Possible fdhF,formate dehydrogenase, highly similar to others formate dehydrogenases and prokaryotic molybdopterin-containing oxidoreductases e.g. Q9S2J9|SC7H2.18 putative formate dehydrogenase from Streptomyces coelicolor (759 aa), FASTA scores: opt: 3038, E(): 2.7e-180, (59.7% identity in 767 aa overlap); Q9HU08|PA5181 probable oxidoreductase from Pseudomonas aeruginosa (773 aa), FASTA scores: opt: 2560,E(): 1.1e-150, (53.2% identity in 761 aa overlap); P78160 formate dehydrogenase a chain (fragment) from Escherichia coli strain K12 (740 aa), FASTA scores: opt: 2002, E(): 3.7e-116, (43.1% identity in 733 aa overlap); P07658|FDHF_ECOLI|P78137|B4079 formate dehydrogenase from Escherichia coli strain K12 (715 aa), FASTA scores: opt: 305, E(): 5.6e-13, (25.5% identity in 748 aa overlap); etc. Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.; Possible formate dehydrogenase H FdhF (formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide) (formate dehydrogenase-H alpha subunit) (FDH-H) 3211745 fdhF 887987 fdhF Mycobacterium tuberculosis H37Rv Possible formate dehydrogenase H FdhF (formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide) (formate dehydrogenase-H alpha subunit) (FDH-H) NP_217416.1 3209406 R 83332 CDS NP_217417.1 15610038 888605 complement(3211803..3212108) 1 NC_000962.3 Rv2901c, (MTCY274.32c), len: 101 aa. Conserved protein, very equivalent to O33023|ML1610|MLCB250.41 hypothetical 12.3 KDA protein from Mycobacterium leprae (101 aa), FASTA scores: opt: 658, E(): 2.6e-43, (99.0% identity in 101 aa overlap). Also highly similar to O69889|SC2E1.18 hypothetical protein from Streptomyces coelicolor and Streptomyces lividans (102 aa), FASTA scores: opt: 515, E(): 2.2e-32, (75.0% identity in 100 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3212108 888605 Rv2901c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217417.1 3211803 R 83332 CDS NP_217418.1 15610039 888504 complement(3212162..3212956) 1 NC_000962.3 Rv2902c, (MT2970, MTCY274.33c), len: 264 aa. Probable rnhB, ribonuclease HII, equivalent to O33022|RNH2_MYCLE|RNHB|ML1611|MLCB250.40 ribonuclease HII from Mycobacterium leprae (240 aa), FASTA scores: opt: 1242, E(): 6.9e-72, (76.75% identity in 245 aa overlap). Also similar (but longer ~20 aa) to others e.g. Q9HXY9|RNHB|PA3642 ribonuclease HII from Pseudomonas aeruginosa (201 aa), FASTA scores: opt: 572, E(): 3.1e-29,(52.7% identity in 184 aa overlap); Q9PEI7|RNH2_XYLFA|RNHB|XF1041 ribonuclease HII from Xylella fastidiosa (234 aa), FASTA scores: opt: 556, E(): 3.6e-28,(50.25% identity in 185 aa overlap); P10442|RNH2_ECOLI|RNHB|B0183 ribonuclease HII from Escherichia coli strain K-12 (213 aa), FASTA scores: opt: 519, E(): 7.4e-26, (48.65% identity in 183 aa overlap); etc. Belongs to the RNASE HII family. Cofactor: manganese (by similarity).; Probable ribonuclease HII protein RnhB (RNase HII) 3212956 rnhB 888504 rnhB Mycobacterium tuberculosis H37Rv Probable ribonuclease HII protein RnhB (RNase HII) NP_217418.1 3212162 R 83332 CDS NP_217419.1 15610040 887157 complement(3212970..3213854) 1 NC_000962.3 Rv2903c, (MTCY274.34c), len: 294 aa. Probable lepB,signal peptidase I (type II membrane protein) (see Braunstein & Belisle 2000), equivalent to O33021|LEP_MYCLE|ML1612|MLCB250.39 probable signal peptidase I from Mycobacterium leprae (289 aa), FASTA scores: opt: 1335, E(): 1.8e-77, (69.75% identity in 301 aa overlap). Also similar to many e.g. O86869|SIPX signal peptidase I from Streptomyces lividans (320 aa), FASTA scores: opt: 474, E(): 1e-22, (43.55% identity in 248 aa overlap); O69884|SIP1|SIPW putative signal peptidase I from Streptomyces coelicolor and Streptomyces lividans (259 aa),FASTA scores: opt: 226, E(): 5e-07, (36.0% identity in 214 aa overlap); P42668|LEP_BACLI|sip signal peptidase I from Bacillus licheniformis (186 aa), FASTA scores: opt: 218,E(): 1.3e-06, (34.5% identity in 194 aa overlap); etc. Contains PS00501 Signal peptidases I serine active site,and PS00761 Signal peptidases I signature 3. Belongs to peptidase family S26; also known as type I leader peptidase family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable signal peptidase I LepB (SPASE I) (leader peptidase I) 3213854 lepB 887157 lepB Mycobacterium tuberculosis H37Rv Probable signal peptidase I LepB (SPASE I) (leader peptidase I) NP_217419.1 3212970 R 83332 CDS NP_217420.1 15610041 887356 complement(3213912..3214253) 1 NC_000962.3 Rv2904c, (MTCY274.35c), len: 113 aa. rplS, 50S ribosomal protein L19, equivalent to O33020|RL19_MYCLE 50S ribosomal protein L19 from Mycobacterium leprae (113 aa),FASTA scores: opt: 702, E(): 1.4e-45, (93.8% identity in 113 aa overlap). Also highly similar to others e.g. O69883|RL19_STRCO from Streptomyces coelicolor (116 aa),FASTA scores: opt: 571, E(): 9.5e-36, (77.25% identity in 110 aa overlap); O31742|RL19_BACSU from Bacillus subtilis (115 aa), FASTA scores: opt: 523, E(): 3.8e-32, (72.9% identity in 107 aa overlap); RL19_BACST|P30529 from Bacillus stearothermophilus (116 aa), FASTA scores: opt: 518, E(): 9.1e-32, (71.7% identity in 106 aa overlap); etc. Belongs to the L19P family of ribosomal proteins.; 50S ribosomal protein L19 RplS 3214253 rplS 887356 rplS Mycobacterium tuberculosis H37Rv 50S ribosomal protein L19 RplS NP_217420.1 3213912 R 83332 CDS NP_217421.1 15610042 887421 3214628..3215572 1 NC_000962.3 Rv2905, (MTCY274.36), len: 314 aa. Probable lppW,conserved ala-rich lipoprotein, with slight similarity to beta-lactamases and hypothetical proteins e.g. Q9S1P7|SCJ9A.23 hypothetical 36.3 KDA protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 222,E(): 2.8e-06, (25.5% identity in 298 aa overlap); O69914|SC3C8.01 putative secreted protein from Streptomyces coelicolor (302 aa), FASTA scores: opt: 201, E(): 5.1e-05,(24.9% identity in 257 aa overlap); P14559|BLAC_STRAL beta-lactamase precursor from Streptomyces albus G (314 aa), FASTA scores: opt: 113, E(): 3.3, (25.2% identity in 278 aa overlap); etc. Has signal peptide and appropriately positioned prokaryotic lipoprotein lipid attachment site: attached to the membrane by a lipid anchor (potential).; Probable conserved alanine rich lipoprotein LppW 3215572 lppW 887421 lppW Mycobacterium tuberculosis H37Rv Probable conserved alanine rich lipoprotein LppW NP_217421.1 3214628 D 83332 CDS NP_217422.1 15610043 888495 complement(3215665..3216357) 1 NC_000962.3 Rv2906c, (MTCY274.37c), len: 230 aa. Probable trmD,tRNA m1G methyltransferase, equivalent to O33017|TRMD_MYCLE from Mycobacterium leprae (238 aa), FASTA scores: opt: 1363, E(): 8.1e-86, (87.2% identity in 227 aa overlap). Also highly similar to others e.g. O69882|TRMD_STRCO from Streptomyces coelicolor and S. lividans (277 aa), FASTA scores: opt: 841, E(): 4.5e-50, (55.55% identity in 234 aa overlap); Q9A0B6 from Streptococcus pyogenes (243 aa),FASTA scores: opt: 698, E(): 2.5e-40, (47.6% identity in 227 aa overlap); P07020|TRMD_ECOLI|TRMD|B2607|Z3901|ECS3470 from Escherichia coli strain O157:H7 (255 aa), FASTA scores: opt: 573, E(): 3.8e-33, (42.1% identity in 228 aa overlap); etc. Belongs to the RNA methyltransferase TRMD family.; Probable tRNA (guanine-N1)-methyltransferase TrmD (M1G-methyltransferase) (tRNA [GM37] methyltransferase) 3216357 trmD 888495 trmD Mycobacterium tuberculosis H37Rv Probable tRNA (guanine-N1)-methyltransferase TrmD (M1G-methyltransferase) (tRNA [GM37] methyltransferase) NP_217422.1 3215665 R 83332 CDS NP_217423.1 15610044 887188 complement(3216361..3216891) 1 NC_000962.3 Rv2907c, (MTCY274.38c), len: 176 aa. Probable rimM,16S rRNA processing protein, equivalent to O33016|RIMM_MYCLE probable 16S rRNA processing protein from Mycobacterium leprae (179 aa), FASTA scores: opt: 797, E(): 2.4e-46, (73.15% identity in 175 aa overlap). Also highly similar to others e.g. O69881|RIMM_STRCO from Streptomyces coelicolor (188 aa), FASTA scores: opt: 485, E(): 2.3e-25,(48.85% identity in 176 aa overlap); Q9KA14|RIMM_BACHD from Bacillus halodurans (173 aa), FASTA scores: opt: 289, E(): 3.2e-12, (30.65% identity in 173 aa overlap); P21504|RIMM_ECOLI|RIMM|B2608 from Escherichia coli strain K12 (182 aa), FASTA scores: opt: 237, E(): 1e-08, (29.4% identity in 177 aa overlap). Belongs to the RimM family.; Probable 16S rRNA processing protein RimM 3216891 rimM 887188 rimM Mycobacterium tuberculosis H37Rv Probable 16S rRNA processing protein RimM NP_217423.1 3216361 R 83332 CDS NP_217424.1 15610045 887336 complement(3216905..3217147) 1 NC_000962.3 Rv2908c, (MTCY274.40c), len: 80 aa. Conserved hypothetical protein, equivalent to O33015|YT08_MYCLE from Mycobacterium leprae (80 aa), FASTA scores: opt: 492, E(): 3.1e-29, (93.75% identity in 80 aa overlap). Also highly similar to others e.g. O69880|YE09_STRCO from Streptomyces coelicolor (79 aa), FASTA scores: opt: 356, E(): 3e-19,(71.6% identity in 74 aa overlap); Q9KA12|BH2482 protein from Bacillus halodurans (76 aa), FASTA scores: opt: 220,E(): 2.9e-09, (48.6% identity in 72 aa overlap); O31738|YLQC_BACSU hypothetical 9.1 KDA protein from Bacillus subtilis (81 aa), FASTA scores: opt: 172, E(): 1e-05, (39.2% identity in 74 aa overlap); etc. Belongs to the UPF0109 family.; hypothetical protein 3217147 887336 Rv2908c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217424.1 3216905 R 83332 CDS NP_217425.1 15610046 888631 complement(3217155..3217643) 1 NC_000962.3 Rv2909c, (MTCY274.41c), len: 162 aa. rpsP, 30S ribosomal protein S16, equivalent to O33014|RS16_MYCLE 30S ribosomal protein S16 from Mycobacterium leprae (160 aa),FASTA scores: opt: 828, E(): 1.6e-39, (82.5% identity in 160 aa overlap). Also highly similar to others e.g. O69879|RS16_STRCO 30S ribosomal protein S16 from Streptomyces coelicolor (139 aa), FASTA scores: opt: 486,E(): 1.9e-20, (56.95% identity in 144 aa overlap); P80379|RS16_THETH 30S ribosomal protein S16 from Thermus Thermophilus (88 aa), FASTA scores: opt: 280, E(): 4.8e-09,(53.25% identity in 77 aa overlap) (C-terminus shorter); P21474|RS16_BACSU|RPSP 30S ribosomal protein S16 (BS17) from Bacillus subtilis (89 aa,), FASTA scores: opt: 258,E(): 8.2e-08, (42.85% identity in 91 aa overlap) (C-terminus shorter); etc. Belongs to the S16P family of ribosomal proteins.; 30S ribosomal protein S16 RpsP 3217643 rpsP 888631 rpsP Mycobacterium tuberculosis H37Rv 30S ribosomal protein S16 RpsP NP_217425.1 3217155 R 83332 CDS NP_217426.1 15610047 887387 complement(3217827..3218270) 1 NC_000962.3 Rv2910c, (MTCY274.42c), len: 147 aa. Conserved hypothetical protein, showing some similarity with hypothetical proteins from other organisms e.g. Q9JN76|MMYY hypothetical 17.4 KDA protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 164, E(): 0.00026, (35.05% identity in 129 aa overlap); etc. Also some similarity with protein from Mycobacterium tuberculosis e.g. O07237|Rv0310c|MTCY63.15c (163 aa), FASTA scores: opt: 165,E(): 0.00023, (26.3% identity in 137 aa overlap); P96815|Rv0138|MTCI5.12 (167 aa), FASTA scores: opt: 132,E(): 0.048, (30.25% identity in 109 aa overlap); etc.; hypothetical protein 3218270 887387 Rv2910c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217426.1 3217827 R 83332 CDS YP_177914.1 57117035 887189 3218339..3219214 1 NC_000962.3 Rv2911, (MTCY274.43), len: 291 aa. Probable dacB2,D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein), an ala-rich protein. Highly similar (except in N-terminus) to Q9CCM2|ML0691 putative D-alanyl-D-alanine carboxypeptidase from Mycobacterium leprae (411 aa), FASTA scores: opt: 749, E(): 9.3e-39, (46.75% identity in 276 aa overlap). Also similar to penicillin binding proteins / D-alanyl-D-alanine carboxypeptidases e.g. Q9KCJ8|SC4G1.16c D-alanyl-D-alanine carboxypeptidase from Streptomyces coelicolor (382 aa), FASTA scores: opt: 386, E(): 2.1e-16,(31.25% identity in 285 aa overlap); P35150|DACB_BACSU penicillin-binding protein 5* precursor from Bacillus subtilis (382 aa), FASTA scores: opt: 384, E(): 3.6e-17,(30.7% identity in 244 aa overlap); Q9K8X5|DACB|BH2877 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) from Bacillus halodurans (395 aa), FASTA scores: opt: 359, E(): 9.7e-15, (30.3% identity in 241 aa overlap); P33364|PBP7_ECOLI|PBPG|B2134 penicillin-binding protein 7 precursor from Escherichia coli strain K12 (313 aa), FASTA scores: opt: 273, E(): 7.5e-10, (27.8% identity in 263 aa overlap); etc. Also similar to O53380|Rv3330|MTV016.30 penicillin-binding protein from Mycobacterium tuberculosis (405 aa), FASTA scores: opt: 746, E(): 1.4e-38, (47.0% identity in 266 aa overlap). Seems to contain PF00768 Peptidase_S11 domain PFAM. Belongs to peptidase family S11; also known as the D-alanyl-D-alanine carboxypeptidase 1 family. Thought to be a membrane-bound protein. Note that previously known as dacB.; Probable penicillin-binding protein DacB2 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase) 3219214 dacB2 887189 dacB2 Mycobacterium tuberculosis H37Rv Probable penicillin-binding protein DacB2 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase) YP_177914.1 3218339 D 83332 CDS NP_217428.1 15610049 888017 complement(3219274..3219861) 1 NC_000962.3 Rv2912c, (MTCY274.44c), len: 195 aa. Probable transcription regulatory protein, TetR family, showing similarity with others e.g. Q9K3V9|SCD10.17 putative TetR-family transcriptional from Streptomyces coelicolor (202 aa), FASTA scores: opt: 185, E(): 4.4e-05, (31.15% identity in 167 aa overlap); Q9KFQ0 TetR-family from Bacillus halodurans (185 aa), FASTA scores: opt: 164, E(): 0.001, (35.6% identity in 73 aa overlap); P17446|BETI_ECOLI|BETI|B0313 regulatory protein from Escherichia coli strain K12 (195 aa), FASTA scores: opt: 126, E(): 0.024, (24.5% identity in 196 aa overlap); etc. Contains possible helix-turn-helix motif at aa 33-54 (+2.71 SD). Possibly belongs to the TetR/AcrR family.; Probable transcriptional regulatory protein (probably TetR-family) 3219861 888017 Rv2912c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR-family) NP_217428.1 3219274 R 83332 CDS NP_217429.1 15610050 887809 complement(3219863..3221698) 1 NC_000962.3 Rv2913c, (MTCY338.01c, MTCY274.45c), len: 611 aa. Possible D-amino acid aminohydrolase, similar (principally in N-terminus) to D-amino acid aminohydrolases e.g. Q9V2D3|NDAD|PAB0090 D-aminoacylase (aspartate, glutamate etc) from Pyrococcus abyssi (526 aa), FASTA scores: opt: 336, E(): 2.2e-13, (27.55% identity in 581 aa overlap); P94212|NDDD_ALCXX N-acyl-D-aspartate deacylase (N-acyl-D-aspartate amidohydrolase) from Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans) (498 aa), FASTA scores: opt: 221, E(): 3.4e-06, (25.95% identity in 532 aa overlap); Q9AGH8 D-aminoacylase from Alcaligenes faecalis (484 aa), FASTA scores: opt: 218, E(): 5.1e-06,(28.35% identity in 434 aa overlap); etc.; Possible D-amino acid aminohydrolase (D-amino acid hydrolase) 3221698 887809 Rv2913c Mycobacterium tuberculosis H37Rv Possible D-amino acid aminohydrolase (D-amino acid hydrolase) NP_217429.1 3219863 R 83332 CDS NP_217430.1 15610051 887642 complement(3221767..3223524) 1 NC_000962.3 Rv2914c, (MTCY338.02c), len: 585 aa. Probable pknI,transmembrane serine/threonine-protein kinase (see citation below), ala-rich protein, highly similar to many in Mycobacterium tuberculosis and other bacteria e.g. Q9RLQ7|MBK putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa), FASTA scores: opt: 376,E(): 1.1e-10, (36.95% identity in 287 aa overlap); P33973|PKN1_MYXXA serine/threonine-protein kinase from Myxococcus xanthus (693 aa), FASTA scores: opt: 286, E(): 5.4e-10, (29.9% identity in 374 aa overlap); P72003|PKNF_MYCTU|Rv1746|MT1788|MTCY28.09 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (476 aa), FASTA scores: opt: 675, E(): 1.7e-24, (39.75% identity in 468 aa overlap); Q10697|PKNJ_MYCTU|Rv2088|MT2149|MTCY49.28 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (589 aa), FASTA scores: opt: 574, E(): 1e-19,(34.85% identity in 479 aa overlap); etc. Equivalent to AAK47308 from Mycobacterium tuberculosis strain CDC1551 (603 aa) but shorter 18 aa. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases.; Probable transmembrane serine/threonine-protein kinase I PknI (protein kinase I) (STPK I) (phosphorylase B kinase kinase) (hydroxyalkyl-protein kinase) 3223524 pknI 887642 pknI Mycobacterium tuberculosis H37Rv Probable transmembrane serine/threonine-protein kinase I PknI (protein kinase I) (STPK I) (phosphorylase B kinase kinase) (hydroxyalkyl-protein kinase) NP_217430.1 3221767 R 83332 CDS NP_217431.1 15610052 888083 complement(3223568..3224680) 1 NC_000962.3 Rv2915c, (MTCY338.03c), len: 370 aa. Conserved protein, posssibly XAA-pro dipeptidase (prolidase), highly similar to CAC38796|SCI39.08c conserved hypothetical protein from Streptomyces coelicolor (363 aa), FASTA scores: opt: 1341, E(): 5.5e-76, (56.65% identity in 362 aa overlap); and similar to prolidases (XAA-pro dipeptidase) e.g. Q9ABC9|CC0300 putative XAA-pro dipeptidase from Caulobacter crescentus (428 aa), FASTA scores: opt: 327,E(): 7.4e-13, (30.2% identity in 374 aa overlap); Q97XD4 prolidase from Sulfolobus solfataricus (396 aa), FASTA scores: opt: 271, E(): 2.1e-09, (30.5% identity in 354 aa overlap); Q9WX55 prolidase from Microbacterium esteraromaticum (393 aa), FASTA scores: opt: 256, E(): 1.8e-08, (27.95% identity in 365 aa overlap); etc. Also similar to O53619|Rv0074|MTV030.18 conserved hypothetical protein from Mycobacterium tuberculosis (411 aa), FASTA scores: opt: 243, E(): 1.2e-07, (27.5% identity in 389 aa overlap).; hypothetical protein 3224680 888083 Rv2915c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217431.1 3223568 R 83332 CDS NP_217432.1 15610053 888242 complement(3224708..3226285) 1 NC_000962.3 Rv2916c, (MTCY338.04c), len: 525 aa. Probable ffh,signal recognition particle (SRP) protein (ala-, gly-,leu-rich protein) (see citation below), equivalent to O33013|SR54_MYCLE signal recognition particle from Mycobacterium leprae (521 aa), FASTA scores: opt: 2968,E(): 1.6e-145, (87.85% identity in 526 aa overlap). Also highly similar to others e.g. O69874|FFH from Streptomyces coelicolor (550 aa), FASTA scores: opt: 2025, E(): 6e-97,(63.8% identity in 519 aa overlap) (N-terminus longer 34 aa); P37105|SR54_BACSU from Bacillus subtilis (446 aa),FASTA scores: opt: 1451, E(): 1.9e-67, (51.5% identity in 435 aa overlap); BAB57399|FFH from Staphylococcus aureus subsp. aureus Mu50 (455 aa), FASTA scores: opt: 1418, E(): 9.4e-66, (48.65% identity in 448 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the SRP family of GTP-binding proteins. Note that signal recognition particle consists of a small cytoplasmic RNA (SC-RNA) molecule and protein FFH. The protein has a two domain structure: the G-domain binds GTP; the M-domain binds the RNA and also binds the signal sequence.; Probable signal recognition particle protein Ffh (fifty-four homolog) (SRP protein) 3226285 ffh 888242 ffh Mycobacterium tuberculosis H37Rv Probable signal recognition particle protein Ffh (fifty-four homolog) (SRP protein) NP_217432.1 3224708 R 83332 CDS NP_217433.1 15610054 887758 3226363..3228243 1 NC_000962.3 Rv2917, (MTCY338.05), len: 626 aa. Conserved hypothetical ala-, arg-rich protein, highly similar (but longer 34 aa) to O33011|ML1624|MLCB250.18C hypothetical 65.2 KDA protein from Mycobacterium leprae (596 aa), FASTA scores: opt: 3117, E(): 9e-183, (79.8% identity in 584 aa overlap). Also highly similar to Q9S2E8|SCE19A.36C hypothetical 66.2 KDA protein from Streptomyces coelicolor (598 aa), FASTA scores: opt: 1921, E(): 1.1e-109, (56.08% identity in 567 aa overlap); and Q9S3Y6|SDRA SDRA protein from Streptomyces coelicolor (597 aa), FASTA scores: opt: 1896, E(): 3.6e-108, (55.75% identity in 567 aa overlap). And shows some similarity with others proteins from other organisms. Equivalent to AAK47311 putative RNA helicase from Mycobacterium tuberculosis strain CDC1551 (602 aa) but longer 24 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Conserved hypothetical alanine and arginine rich protein 3228243 887758 Rv2917 Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine and arginine rich protein NP_217433.1 3226363 D 83332 CDS NP_217434.1 15610055 888621 complement(3228254..3230680) 1 NC_000962.3 Rv2918c, (MTCY338.07c), len: 808 aa. Probable glnD,uridylyltransferase (ala-rich protein), similar to other uridylyltransferases e.g. O69873||SC2E1.02 from Streptomyces coelicolor (835 aa), FASTA scores: opt: 1473,E(): 2.8e-81, (41.03% identity in 858 aa overlap); P43919|GLND_HAEIN from Haemophilus influenzae (863 aa),FASTA scores: opt: 333, E(): 2.5e-12, (25.4% identity in 819 aa overlap); P27249|GLND_ECOLI|GLND|B0167 from Escherichia coli strain K12 (890 aa), FASTA scores: opt: 306, E(): 1.1e-10, (27.75% identity in 858 aa overlap); etc. Belongs to the GlnD family.; Probable [protein-PII] uridylyltransferase GlnD (PII uridylyl-transferase) (uridylyl removing enzyme) (UTASE) 3230680 glnD 888621 glnD Mycobacterium tuberculosis H37Rv Probable [protein-PII] uridylyltransferase GlnD (PII uridylyl-transferase) (uridylyl removing enzyme) (UTASE) NP_217434.1 3228254 R 83332 CDS NP_217435.1 15610056 887756 complement(3230738..3231076) 1 NC_000962.3 Rv2919c, (MTCY338.08c), len: 112 aa. Probable glnB,nitrogen regulatory protein, highly similar to others e.g. Q9X705|GLNB PII protein from Corynebacterium glutamicum (Brevibacterium flavum) (112 aa), FASTA scores: opt: 531,E(): 4.5e-30, (68.75% identity in 112 aa overlap); P21193|GLNB_AZOBR nitrogen regulatory protein P-II from Azospirillum brasilense (112 aa), FASTA scores: opt: 496,E(): 1.2e-27, (60.7% identity in 112 aa overlap); P05826|GLNB_ECOLI|B2553|Z3829|ECS3419|STY2808 nitrogen regulatory protein P-II from Escherichia coli strains K12 and O157:H7 (112 aa), FASTA scores: opt: 487, E(): 5.3e-27,(61.6% identity in 112 aa overlap); etc. Contains PS00496 P-II protein urydylation site. Belongs to the P(II) protein family.; Probable nitrogen regulatory protein P-II GlnB 3231076 glnB 887756 glnB Mycobacterium tuberculosis H37Rv Probable nitrogen regulatory protein P-II GlnB NP_217435.1 3230738 R 83332 CDS NP_217436.1 15610057 887683 complement(3231073..3232506) 1 NC_000962.3 Rv2920c, (MTCY338.09c), len: 477 aa. Probable amt,ammonium-transport integral membrane protein (ala-, gly-,leu-, val-rich protein), highly similar to others e.g. Q9ZBP6|SC7A1.27 ammonium transporter from Streptomyces coelicolor (448 aa), FASTA scores: opt: 1246, E(): 7.3e-67,(54.1% identity in 462 aa overlap); P54146|AMT_CORGL ammonium transport system from Corynebacterium glutamicum (452 aa), FASTA scores: opt: 953, E(): 2.1e-49, (41.45% identity in 475 aa overlap); Q07429|NRGA_BACSU probable ammonium transporter (membrane protein NRGA) from Bacillus subtilis (404 aa), FASTA scores: opt: 721, E(): 0, (44.4% identity in 430 aa overlap); etc. Belongs to the AMT1/MEP/NRGA family of ammonium transporters (TC 2.49).; Probable ammonium-transport integral membrane protein Amt 3232506 amt 887683 amt Mycobacterium tuberculosis H37Rv Probable ammonium-transport integral membrane protein Amt NP_217436.1 3231073 R 83332 CDS NP_217437.1 15610058 887205 complement(3232871..3234139) 1 NC_000962.3 Rv2921c, (MTCY338.10c, MT2989), len: 422 aa. Probable ftsY, signal recognition particle (SRP) receptor,a membrane-associated cell division protein (see citation below), equivalent to O33010|FTSY_MYCLE cell division protein FTSY homolog from Mycobacterium leprae (430 aa),FASTA scores: opt: 1760, E(): 1.1e-108, (81.35% identity in 429 aa overlap). Also similar to others e.g. Q9I6C1|FTSY|PA0373 signal recognition particle receptor FTSY from Pseudomonas aeruginosa (455 aa), FASTA scores: opt: 882, E(): 5.1e-40, (42.08% identity in 385 aa overlap); Q9KVJ6|FTSY cell division protein from Vibrio cholerae (391 aa), FASTA scores: opt: 837, E(): 1.2e-37,(36.3% identity in 394 aa overlap); P10121|FTSY_ECOLI|FTSY|B3464 cell division protein from Escherichia coli strain K12 (497 aa), FASTA scores: opt: 800, E(): 1.3e-35, (39.75% identity in 327 aa overlap); etc. Also similar to Q9ZBP9|SC7A1.24 putative prokaryotic docking protein from Streptomyces coelicolor (412 aa),FASTA scores: opt: 1461, E(): 4.3e-71, (60.3% identity in 423 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00300 SRP54-type proteins GTP-binding domain signature. Belongs to the SRP family of GTP-binding proteins.; Probable cell division protein FtsY (SRP receptor) (signal recognition particle receptor) 3234139 ftsY 887205 ftsY Mycobacterium tuberculosis H37Rv Probable cell division protein FtsY (SRP receptor) (signal recognition particle receptor) NP_217437.1 3232871 R 83332 CDS NP_217438.2 57117036 887179 complement(3234189..3237806) 1 NC_000962.3 Rv2922c, (MT2990, MTCY338.11c), len: 1205 aa. Probable smc, chromosome partition protein (ala-, arg-,leu-, glu-rich protein, possibly coiled-coil protein) (see * below), equivalent (but longer 84 aa) to Q9CBT5|SMC|ML1629|MLCB250.01 possible cell division protein from Mycobacterium leprae (1203 aa), FASTA scores: opt: 5957, E(): 0, (79.15% identity in 1205 aa overlap). Also highly similar to other chromosome segregation proteins e.g. Q9ZBQ2|SC7A1.21 putative chromosome associated protein from Streptomyces coelicolor (1186 aa), FASTA scores: opt: 2633, E(): 4.1e-120, (53.03% identity in 1205 aa overlap); P51834|SMC_BACSU chromosome partition protein from Bacillus subtilis (1186 aa), FASTA scores: opt: 1009, E(): 2.1e-41,(30.75% identity in 1205 aa overlap); Q9CHC9|SMC chromosome segregation protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (924 aa), FASTA scores: opt: 996,E(): 7.5e-41, (29.75% identity in 874 aa overlap); etc. Equivalent to AAK47317 from Mycobacterium tuberculosis strain CDC1551 (1205 aa) but longer 84 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the SMC family. N-terminus shortened since first submission. [* Note: Unpublished. Cobbe N., Heck M.M.S.-Phylogenetic analysis of SMC proteins (OCT-2001)].; Probable chromosome partition protein Smc 3237806 smc 887179 smc Mycobacterium tuberculosis H37Rv Probable chromosome partition protein Smc NP_217438.2 3234189 R 83332 CDS YP_177679.1 57117037 3205043 complement(3237818..3238099) 1 NC_000962.3 Rv2922A, len: 93 aa. Probable acyP, acylphosphatase (acylphosphate phosphohydrolase), highly similar to others e.g. Q9ZBQ3|SC7A1.20 putative acylphosphatase from Streptomyces coelicolor (93 aa), FASTA scores: opt: 345,E(): 9.5e-19, (58.9% identity in 90 aa overlap); P75877|ACYP_ECOLI|YCCX|B0968|Z1320|ECS1052 putative acylphosphatase from Escherichia coli strains K12 and O157:H7 (92 aa), FASTA scores: opt: 220, E(): 2e-09,(44.95% identity in 89 aa overlap); Q9RVU3|DR0929 putative acylphosphatase from Deinococcus radiodurans (87 aa), FASTA scores: opt: 193, E(): 2.1e-07, (44.3% identity in 79 aa overlap); etc. Belongs to the acylphosphatase family.; Probable acylphosphatase AcyP (acylphosphate phosphohydrolase) 3238099 acyP 3205043 acyP Mycobacterium tuberculosis H37Rv Probable acylphosphatase AcyP (acylphosphate phosphohydrolase) YP_177679.1 3237818 R 83332 CDS NP_217439.1 15610060 887687 complement(3238086..3238499) 1 NC_000962.3 Rv2923c, (MTCY338.12c), len: 137 aa. Conserved protein, showing similarity with other hypothetical proteins e.g. P24246|YHFA_ECOLI|B3356|Z4717|ECS4207 from Escherichia coli strains K12 and O157:H7 (134 aa), FASTA scores: opt: 110, E(): 1.9, (25.9% identity in 135 aa overlap); etc.; hypothetical protein 3238499 887687 Rv2923c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217439.1 3238086 R 83332 CDS NP_217440.1 15610061 887438 complement(3238601..3239470) 1 NC_000962.3 Rv2924c, (MTCY338.13c), len: 289 aa. Probable fpg (alternate gene name: mutM), formamidopyrimidine-DNA glycosylase (see citation below), equivalent to O69470|FPG_MYCLE formamidopyrimidine-DNA glycosylase from Mycobacterium leprae (282 aa), FASTA scores: opt: 1563,E(): 1.3e-96, (80.6% identity in 289 aa overlap). Also highly similar to other formamidopyrimidine-DNA glycosylases e.g. Q9ZBQ6|FPG_STRCO from Streptomyces coelicolor (286 aa), FASTA scores: opt: 1047, E(): 2.9e-62,(57.55% identity in 292 aa overlap); P95744|FPG_SYNEN from Synechococcus elongatus naegeli (284 aa), FASTA scores: opt: 569, E(): 1.9e-30, (37.95% identity in 290 aa overlap); P05523|FPG_ECOLI|MUTM|FPG|B3635 from Escherichia coli strain K12 (269 aa), FASTA scores: opt: 424, E(): 8.2e-21, (33.9% identity in 289 aa overlap); etc. Belongs to the FPG family. Cofactor: binds 1 zinc ion.; Probable formamidopyrimidine-DNA glycosylase Fpg (FAPY-DNA glycosylase) 3239470 fpg 887438 fpg Mycobacterium tuberculosis H37Rv Probable formamidopyrimidine-DNA glycosylase Fpg (FAPY-DNA glycosylase) NP_217440.1 3238601 R 83332 CDS NP_217441.1 15610062 887873 complement(3239829..3240551) 1 NC_000962.3 Rv2925c, (MTCY338.14c), len: 240 aa. Probable rnc,ribonuclease III (RNase III), equivalent to O69469|RNC_MYCLE ribonuclease III from Mycobacterium leprae (238 aa). Also highly similar to other ribonucleases III e.g. Q9ZBQ7|RNC_STRCO from Streptomyces coelicolor (272 aa), FASTA scores: opt: 889, E(): 5.4e-51, (62.2% identity in 225 aa overlap) (N-terminus longer 21 aa); P51833|RNC_BACSU from Bacillus subtilis (249 aa), FASTA scores: opt: 493, E(): 5e-25, (43.25% identity in 215 aa overlap); P05797|RNC_ECOLI|RNC|B2567|Z3848|ECS3433 from Escherichia coli strain O157:H7 and K12 (226 aa), FASTA scores: opt: 459, E(): 7.9e-23, (41.8% identity in 213 aa overlap); etc. Contains PS00517 Ribonuclease III family signature.; Probable ribonuclease III Rnc (RNase III) 3240551 rnc 887873 rnc Mycobacterium tuberculosis H37Rv Probable ribonuclease III Rnc (RNase III) NP_217441.1 3239829 R 83332 CDS NP_217442.1 15610063 887487 complement(3240548..3241171) 1 NC_000962.3 Rv2926c, (MTCY338.15c), len: 207 aa. Conserved protein, equivalent to O69468|ML1660|MLCB1243.14 hypothetical 23.5 KDA protein from Mycobacterium leprae (217 aa), FASTA scores: opt: 866, E(): 1.4e-48, (67.2% identity in 192 aa overlap). Also similar in part to other hypothetical proteins e.g. Q9WXZ8 conserved hypothetical protein from Thermotoga maritima (182 aa), FASTA scores: opt: 254, E(): 3.4e-09, (31.45% identity in 143 aa overlap); Q9ZBQ9|SC7A1.14 hypothetical 23.5 KDA protein from Streptomyces coelicolor (217 aa), FASTA scores: opt: 244, E(): 1.7e-08, (45.5% identity in 189 aa overlap); O65982 hypothetical 26.2 KDA protein from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (228 aa), FASTA scores: opt: 220,E(): 6.1e-07, (32.45% identity in 148 aa overlap); etc. Equivalent to AAK47323 from Mycobacterium tuberculosis strain CDC1551 (195 aa) but longer 12 aa.; hypothetical protein 3241171 887487 Rv2926c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217442.1 3240548 R 83332 CDS NP_217443.1 15610064 887317 complement(3241222..3241959) 1 NC_000962.3 Rv2927c, (MTCY338.16c), len: 245 aa. Conserved hypothetical protein, equivalent to Q9CBS6|ML1661|MLCB1243.13 (alias O69467) hypothetical protein from Mycobacterium leprae (247 aa), FASTA scores: opt: 1440, E(): 4.9e-76, (90.6% identity in 245 aa overlap). Also similar to many hypothetical proteins from other organisms e.g. Q9ZBR0|SC7A1.13 hypothetical 41.0 KDA protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 266, E(): 3.4e-08, (29.9% identity in 234 aa overlap); etc. Also some similarity with P46815|AG84_MYCLE|ML0922 antigen 84 from Mycobacterium leprae (266 aa), FASTA scores: opt: 193, E(): 0.00043,(28.7% identity in 136 aa overlap) (see citation below); and P46816|AG84_MYCTU|WAG31|Rv2145c|MT2204|MTCY270.23 antigen 84 from Mycobacterium tuberculosis (260 aa), FASTA scores: opt: 178, E(): 0.0031, (34.35% identity in 131 aa overlap) (see citation below). Contains potential coiled-coil region.; hypothetical protein 3241959 887317 Rv2927c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217443.1 3241222 R 83332 CDS NP_217444.1 15610065 887446 3242198..3242983 1 NC_000962.3 Rv2928, (MTCY338.17), len: 261 aa. Probable tesA,thioesterase, similar to many e.g. Q9L4W2|NYSE thioesterase involved in synthesis of the polyene antifungal antibiotic nystatin from Streptomyces noursei (see Brautaset et al.,2000) (251 aa). TesA|Rv2928 interacts with PpsE|Rv2935, by bacterial two-hybrid and GST-pulldown assays (See Rao and Ranganathan, 2004).; Probable thioesterase TesA 3242983 tesA 887446 tesA Mycobacterium tuberculosis H37Rv Probable thioesterase TesA NP_217444.1 3242198 D 83332 CDS NP_217445.1 15610066 888112 3242970..3243281 1 NC_000962.3 Rv2929, (MTCY338.18), len: 103 aa. Hypothetical unknown protein; some weak similarity to C-terminal half of P18319|UREG_KLEAE urease accessory protein from klebsiella aerogenes (205 aa), FASTA scores: opt: 99, E(): 1.1, (38.6% identity in 57 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3243281 888112 Rv2929 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217445.1 3242970 D 83332 CDS NP_217446.2 57117038 887603 3243697..3245448 1 NC_000962.3 Rv2930, (MT2999, MTCY338.19), len: 583 aa. FadD26,fatty-acid-AMP synthetase, equivalent to Q9Z5K5|FADD26|ML2358|MLCB12.03c probable acyl-CoA synthase from Mycobacterium leprae (583 aa), FASTA scores: opt: 3026, E(): 9.2e-180, (76.85% identity in 583 aa overlap). Also highly similar to many e.g. Q9CD84|ML0132 putative acyl-CoA synthetase from Mycobacterium leprae (680 aa),FASTA scores: opt: 2324, E(): 3.2e-136, (61.35% identity in 572 aa overlap); P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2304, E(): 5e-135, (59.85% identity in 583 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q50586|FD25_MYCTU|RV1521|MTCY19G5.07 putative fatty-acid--CoA ligase (583 aa), FASTA scores: opt: 2188,E(): 7.6e-128, (57.55% identity in 584 aa overlap); etc. Belongs to the ATP-dependent AMP-binding enzyme family. N-terminus shortened since first submission. Note that Rv2930|fadD26 belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Fatty-acid-AMP ligase FadD26 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 3245448 fadD26 887603 fadD26 Mycobacterium tuberculosis H37Rv Fatty-acid-AMP ligase FadD26 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_217446.2 3243697 D 83332 CDS NP_217447.1 15610068 888183 3245445..3251075 1 NC_000962.3 Rv2931, (MTCY338.20), len: 1876 aa. PpsA, type-I polyketide synthase (see citations below), highly similar to others from Mycobacterium leprae e.g. Q9Z5K6|ML2357|MLCB12.02c putative polyketide synthase from Mycobacterium leprae (1871 aa), FASTA scores: opt: 7566,E(): 0, (76.1% identity in 1888 aa overlap); Q9S384|ML2356|MLCB12.01c putative polyketide synthase from Mycobacterium leprae (1540 aa), FASTA scores: opt: 4026,E(): 9.8e-212, (45.7% identity in 1811 aa overlap); Q49932|PKSC|L518_F1_2 putative polyketide synthase (1446 aa), FASTA scores: opt: 4026, E(): 9.4e-212, (70.6% identity in 885 aa overlap). Also similar to polyketide synthases from other bacteria e.g. C-terminus of Q9L8C7|EPOC polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 2592, E(): 5.2e-133, (32.55% identity in 2245 aa overlap); P22367|MSAS_PENPA 6-methylsalicylic acid synthase from Penicillium patulum (Penicillium griseofulvum) (1774 aa), FASTA scores: opt: 2391, E(): 0, (34.2% identity in 1815 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. Q10978|PPSB_MYCTU|RV2932 phenolpthiocerol synthesis polyketide synthase (1538 aa), FASTA scores: opt: 4227, E(): 0, (46.8% identity in 1810 aa overlap) (gap in middle); etc. Contains PS00606 Beta-ketoacyl synthases active site, and PS00012 Phosphopantetheine attachment site. Note that Rv2931|ppsA belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Phenolpthiocerol synthesis type-I polyketide synthase PpsA 3251075 ppsA 888183 ppsA Mycobacterium tuberculosis H37Rv Phenolpthiocerol synthesis type-I polyketide synthase PpsA NP_217447.1 3245445 D 83332 CDS NP_217448.1 15610069 888023 3251072..3255688 1 NC_000962.3 Rv2932, (MTV011.01, MTCY338.21, MT3002), len: 1538 aa. PpsB, type-I polyketide synthase (see citations below),highly similar to others from Mycobacterium leprae e.g. Q9S384|ML2356|MLCB12.01c putative polyketide synthase (1540 aa), FASTA scores: opt: 7284, E(): 0, (76.3% identity in 1561 aa overlap); Q49932|PKSC|L518_F1_2 putative polyketide synthase (1446 aa), FASTA scores: opt: 6811, E(): 0, (76.2% identity in 1462 aa overlap); etc. Also similar to polyketide synthases from other bacteria e.g. Q9KIZ6|EPOE EPOE protein from Polyangium cellulosum (3798 aa), FASTA scores: opt: 3052, E(): 3.3e-165, (38.35% identity in 1538 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. Q10977|PPSA_MYCTU|RV2931 phenolpthiocerol synthesis polyketide synthase (1876 aa),FASTA scores: opt: 4227, E(): 0, (46.9% identity in 1810 aa overlap); P96203|PPSD|Rv2934|MTCY19H9.02 PKSE protein (1827 aa), FASTA scores: opt: 3756, E(): 1.8e-205, (42.9% identity in 1808 aa overlap); etc. Overlaps and extends CDS from neighbouring cosmid MTCY338.21. Contains PS00606 Beta-ketoacyl synthases active site. Note that Rv2932|ppsB belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). Nucleotide position 3254365 in the genome sequence has been corrected, T:C resulting in L1098L.; Phenolpthiocerol synthesis type-I polyketide synthase PpsB 3255688 ppsB 888023 ppsB Mycobacterium tuberculosis H37Rv Phenolpthiocerol synthesis type-I polyketide synthase PpsB NP_217448.1 3251072 D 83332 CDS NP_217449.1 15610070 887686 3255685..3262251 1 NC_000962.3 Rv2933, (MTCY19H9.01, MTV011.02), len: 2188 aa. ppsC, type-I polyketide synthase (see citations below),highly similar to others from Mycobacterium leprae e.g. Q49933|PKSD|ML2355|L518_F1_3 putative polyketide synthase (2201 aa), FASTA scores: opt: 6973, E(): 0, (82.32% identity in 2217 aa overlap); Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase (2118 aa), FASTA scores: opt: 4015, E(): 2.9e-208, (36.6% identity in 2184 aa overlap); etc. Also similar to polyketide synthases from other bacteria e.g. C-terminus of Q9L8C7 polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 3909, E(): 3.6e-202, (40.15% identity in 2220 aa overlap); Q9KIZ7|EPOD EPOD protein from Polyangium cellulosum (7257 aa), FASTA scores: opt: 3886, E(): 6.2e-201, (40.05% identity in 2220 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. P96291|Rv2940c (2111 aa),FASTA scores: opt: 4204, E(): 0, (39.1% identity in 2176 aa overlap); Q10977|PPSA_MYCTU|RV2931 phenolpthiocerol synthesis polyketide synthase (1876 aa), FASTA scores: opt: 3793, E(): 2.4e-196, (46.65% identity in 1612 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site,and PS00012 Phosphopantetheine attachment site. Note that Rv2933|ppsC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Phenolpthiocerol synthesis type-I polyketide synthase PpsC 3262251 ppsC 887686 ppsC Mycobacterium tuberculosis H37Rv Phenolpthiocerol synthesis type-I polyketide synthase PpsC NP_217449.1 3255685 D 83332 CDS NP_217450.1 15610071 887172 3262248..3267731 1 NC_000962.3 Rv2934, (MTCY19H9.02), len: 1827 aa. PpsD, type-I polyketide synthase (see citations below), highly similar to others from Mycobacterium leprae e.g. Q9CB70|ML2354 polyketide synthase (1822 aa), FASTA scores: opt: 9779,E(): 0, (80.35% identity in 1836 aa overlap); Q49940|L518_F3_67|PFSE (1815 aa), FASTA scores: opt: 9658,E(): 0, (79.85% identity in 1831 aa overlap); etc. Also similar to polyketide synthases from other bacteria e.g. C-terminus of Q9RNB2|MCYD|Q9FDU1 polyketide synthase (MCYD protein) from Microcystis aeruginosa (3906 aa), FASTA scores: opt: 2961, E(): 6e-159, (32.15% identity in 1827 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. Q10978|PPSB_MYCTU|RV2932 phenolpthiocerol synthesis polyketide synthase (1538 aa),FASTA scores: opt: 3756, E(): 3.8e-204, (42.85% identity in 1808 aa overlap) (gaps in middle); P96202|PPSC|RV2933 polyketide synthase (2188 aa), FASTA scores: opt: 3463,E(): 1.7e-187, (39.2% identity in 2165 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site,PS00017 ATP/GTP-binding site motif A, PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and PS00012 Phosphopantetheine attachment site. Note that Rv2934|ppsD belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Phenolpthiocerol synthesis type-I polyketide synthase PpsD 3267731 ppsD 887172 ppsD Mycobacterium tuberculosis H37Rv Phenolpthiocerol synthesis type-I polyketide synthase PpsD NP_217450.1 3262248 D 83332 CDS NP_217451.1 15610072 888210 3267737..3272203 1 NC_000962.3 Rv2935, (MTCY19H9.03), len: 1488 aa. PpsE, type-I polyketide synthase (see citations below). Contains PS00606 Beta-ketoacyl synthases active site. Note that Rv2935|ppsE belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). TesA|Rv2928 interacts with PpsE|Rv2935, by bacterial two-hybrid and GST-pulldown assays (See Rao and Ranganathan, 2004).; Phenolpthiocerol synthesis type-I polyketide synthase PpsE 3272203 ppsE 888210 ppsE Mycobacterium tuberculosis H37Rv Phenolpthiocerol synthesis type-I polyketide synthase PpsE NP_217451.1 3267737 D 83332 CDS NP_217452.1 15610073 888168 3272214..3273209 1 NC_000962.3 Rv2936, (MTCY19H9.04), len: 331 aa. drrA,daunorubicin-dim-transport resistance ATP-binding protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q49938|DRRA|ML2352|L518_F2_43|DRRA probable daunorubicin resistance ATP-binding protein from Mycobacterium leprae (331 aa), FASTA scores: opt: 1842, E(): 4.2e-103, (85.2% identity in 331 aa overlap). Also highly similar to others e.g. Q9XCF7 DRRA from Mycobacterium avium (315 aa), FASTA scores: opt: 1040, E(): 4.7e-55, (54.35% identity in 309 aa overlap); Q9X5J8 daunorubicin resistance protein A from Mycobacterium avium (315 aa), FASTA scores: opt: 1030, E(): 1.9e-54, (53.7% identity in 309 aa overlap); P32010|DRRA_STRPE daunorubicin resistance ATP-binding protein from Streptomyces peucetius (330 aa), FASTA scores: opt: 852, E(): 9e-44, (47.15% identity in 318 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Note that Rv2936|drrA belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Daunorubicin-dim-transport ATP-binding protein ABC transporter DrrA 3273209 drrA 888168 drrA Mycobacterium tuberculosis H37Rv Daunorubicin-dim-transport ATP-binding protein ABC transporter DrrA NP_217452.1 3272214 D 83332 CDS NP_217453.1 15610074 887968 3273206..3274075 1 NC_000962.3 Rv2937, (MTCY19H9.05), len: 289 aa. drrB,daunorubicin-dim-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q49935|DRRB|ML2351|L518_F1_9 daunorubicin resistance transmembrane protein from Mycobacterium leprae (288 aa), FASTA scores: opt: 1252,E(): 5.3e-72, (64.0% identity in 289 aa overlap). Also similar to others e.g. Q9XCF8 DRRB protein from Mycobacterium avium (246 aa), FASTA scores: opt: 423, E(): 1.5e-19, (30.85% identity in 243 aa overlap); Q9S6H4 daunorubicin resistance protein B from Mycobacterium avium (246 aa), FASTA scores: opt: 420, E(): 2.3e-19, (30.85% identity in 243 aa overlap); P32011|DRRB_STRPE daunorubicin resistance transmembrane protein from Streptomyces peucetius (283 aa), FASTA scores: opt: 242, E(): 4.7e-08,(27.85% identity in 219 aa overlap); etc. Note that Rv293|drrB belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Daunorubicin-dim-transport integral membrane protein ABC transporter DrrB 3274075 drrB 887968 drrB Mycobacterium tuberculosis H37Rv Daunorubicin-dim-transport integral membrane protein ABC transporter DrrB NP_217453.1 3273206 D 83332 CDS NP_217454.1 15610075 888491 3274072..3274902 1 NC_000962.3 Rv2938, (MTCY19H9.06), len: 276 aa. Probable drrC,daunorubicin-dim-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q9CB71|ML2350 probable antibiotic resistance membrane protein from Mycobacterium leprae (276 aa), FASTA scores: opt: 1434, E(): 1.2e-81, (79.0% identity in 276 aa overlap); and Q49941|DRRC|L518_F3_76 putative daunorubicin resistance transmembrane protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1194,E(): 8.3e-67, (76.85% identity in 242 aa overlap). Also similar to others e.g. Q9XCF9 DRRC protein from Mycobacterium avium (263 aa), FASTA scores: opt: 538, E(): 3.7e-26, (32.65% identity in 251 aa overlap); Q9S6H3 daunorubicin resistance protein C from Mycobacterium avium (263 aa), FASTA scores: opt: 533, E(): 7.6e-26, (32.25% identity in 251 aa overlap); P32011|DRRB_STRPE daunorubicin resistance transmembrane protein from Streptomyces peucetius (283 aa), FASTA scores: opt: 276, E(): 6.6e-10,(21.07% identity in 261 aa overlap); etc. Note that Rv2938|drrC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Probable daunorubicin-dim-transport integral membrane protein ABC transporter DrrC 3274902 drrC 888491 drrC Mycobacterium tuberculosis H37Rv Probable daunorubicin-dim-transport integral membrane protein ABC transporter DrrC NP_217454.1 3274072 D 83332 CDS NP_217455.1 15610076 887327 3274949..3276217 1 NC_000962.3 Rv2939, (MTCY19H9.07), len: 422 aa. Possible papA5,conserved polyketide synthase (PKS) associated protein (see Camacho et al., 2001), equivalent to Q49939 hypothetical 45.6 KDA protein from Mycobacterium leprae (423 aa), FASTA scores: opt: 2398, E(): 4.5e-144, (84.05% identity in 426 aa overlap); and Q02279|YMA3_MYCBO hypothetical 38.1 KDA protein from Mycobacterium bovis (354 aa), FASTA scores: opt: 2193, E(): 3.6e-131, (97.4% identity in 343 aa overlap). And C-terminus highly similar to to Q9S381 hypothetical 5.0 KDA protein (fragment) from Mycobacterium leprae (44 aa), FASTA scores: opt: 275, E(): 1.4e-10,(88.65% identity in 44 aa overlap). Also similar in part to various synthetases e.g. Q9AE01|RIF20 RIF20 protein from Amycolatopsis mediterranei (Nocardia mediterranei) (403 aa), FASTA scores: opt: 282, E(): 2.7e-10, (30.3% identity in 393 aa overlap); middle part of Q00869|ESYN1 enniatin sythetase (fragment) (N-methyl peptide synthetase) from Fusarium equiseti (3131 aa), FASTA scores: opt: 180, E(): 0.0036, (26.85% identity in 242 aa overlap); N-terminus of Q9FB18 peptide synthetase NRPS2-1 from Streptomyces verticillus (2626 aa), FASTA scores: opt: 159, E(): 0.068,(23.65% identity in 351 aa overlap); etc. Note that Rv2939|papA5 belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally).; Possible conserved polyketide synthase associated protein PapA5 3276217 papA5 887327 papA5 Mycobacterium tuberculosis H37Rv Possible conserved polyketide synthase associated protein PapA5 NP_217455.1 3274949 D 83332 CDS NP_217456.1 15610077 887982 complement(3276380..3282715) 1 NC_000962.3 Rv2940c, (MTCY24G1.09, MTCY19H9.08c), len: 2111 aa. Probable mas, mycocerosic acid synthase membrane associated, multifunctional enzyme (see citations below),almost identical to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 13226, E(): 0, (95.8% identity in 2115 aa overlap) (see Mathur & Kolattukudy 1992); and equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 12142,E(): 0, (87.95% identity in 2119 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase from Mycobacterium leprae (2118 aa), FASTA scores: opt: 8421, E(): 0, (60.8% identity in 2127 aa overlap). Also similar to other synthases e.g. C-terminus of Q9L8C7|EPOC polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 4332, E(): 0,(40.85% identity in 2149 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. O53901|PKS5|Rv1527c|MTV045.01c|MTCY19G5.01 polyketide synthase (2108 aa), FASTA scores: opt: 5059, E(): 0, (65.9% identity in 2121 aa overlap); etc. Contains several domains, organized in the following order: beta-ketoacyl synthase (PS00606), acyl transferase, dehydratase-enoyl reductase, beta-ketoreductase, acyl carrier protein. Contains PS00012 Phosphopantetheine attachment site.; Probable multifunctional mycocerosic acid synthase membrane-associated Mas 3282715 mas 887982 mas Mycobacterium tuberculosis H37Rv Probable multifunctional mycocerosic acid synthase membrane-associated Mas NP_217456.1 3276380 R 83332 CDS NP_217457.1 15610078 887454 3283335..3285077 1 NC_000962.3 Rv2941, (MTCY24G1.08c), len: 580 aa. FadD28 (alternate gene name: acoas), fatty-acid-AMP synthetase (see citations below), almost identical to P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 3828, E(): 0, (99.15% identity in 580 aa overlap); and equivalent to Q9CD79|FADD28|ML0138 acyl-CoA synthetase from Mycobacterium leprae (579 aa), FASTA scores: opt: 3183,E(): 8.8e-186, (81.9% identity in 580 aa overlap). And also highly similar to others Mycobacteria proteins e.g. O07797|FADD23|Rv3826|MTCY409.04c putative fatty-acid-CoA synthetase from Mycobacterium tuberculosis (584 aa); etc. Contains PS00018 EF-hand calcium-binding domain. Note that Rv2941|fadD28 and Rv2942|mmpL7 are transcriptionally coupled (proven experimentally).; Fatty-acid-AMP ligase FadD28 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 3285077 fadD28 887454 fadD28 Mycobacterium tuberculosis H37Rv Fatty-acid-AMP ligase FadD28 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_217457.1 3283335 D 83332 CDS NP_217458.1 15610079 887548 3285070..3287832 1 NC_000962.3 Rv2942, (MTCY24G1.07c), len: 920 aa. MmpL7,conserved transmembrane transport protein (see citations below), member of RND superfamily, highly similar to Q9XB10 hypothetical 99.5 KDA protein from Mycobacterium bovis BCG (945 aa), FASTA scores: opt: 488, E(): 4.9e-20, (29.5% identity in 918 aa overlap); and to others from Mycobacteria e.g. O53735|MML4_MYCTU from Mycobacterium tuberculosis (945 aa), FASTA scores: opt: 481, E(): 1.2e-19, (25.9% identity in 922 aa overlap); etc. Also similar to other membrane proteins e.g. O54101|MMLB_STRCO|SC10A5.10c putative membrane protein from Streptomyces coelicolor (847 aa), FASTA scores: opt: 256,E(): 7.2e-07, (25.15% identity in 545 aa overlap); etc. Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site, PS00079 Multicopper oxidases signature 1, and PS00044 Bacterial regulatory proteins,lysR family signature. Belongs to the MmpL family. Note that Rv2941|fadD28 and Rv2942|mmpL7 are transcriptionally coupled (proven experimentally).; Conserved transmembrane transport protein MmpL7 3287832 mmpL7 887548 mmpL7 Mycobacterium tuberculosis H37Rv Conserved transmembrane transport protein MmpL7 NP_217458.1 3285070 D 83332 CDS NP_217459.1 15610080 887834 3288464..3289705 1 NC_000962.3 Rv2943, (MTCY24G1.06c), len: 413 aa. Probable transposase for insertion sequence IS1533, similar to other transposases e.g. P15025|ISTA_ECOLI ista protein (insertion sequence IS21) from Escherichia coli (390 aa), FASTA scores: opt: 268, E(): 5.1e-11, (24.1% identity in 378 aa overlap). Contains potential helix-turn-helix motif at aa 19-40 (Score 1611, +4.67 SD).; Probable transposase for insertion sequence element IS1533 3289705 887834 Rv2943 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS1533 NP_217459.1 3288464 D 83332 CDS YP_177680.1 57117039 3205061 3289705..3290235 1 NC_000962.3 Rv2943A, len: 176 aa. Possible transposase, similar to many e.g. AJ238712|MBO238712_2 putative transposase (IS21-l) from Mycobacterium bovis BCG (266 aa), FASTA scores: opt: 762, E(): 0, (100.0% identity in 118 aa overlap). Possible frameshift after codon 118 i.e. near position 3290056, to fuse with Rv2944.; Possible transposase 3290235 3205061 Rv2943A Mycobacterium tuberculosis H37Rv Possible transposase YP_177680.1 3289705 D 83332 CDS NP_217460.1 15610081 887636 3289790..3290506 1 NC_000962.3 Rv2944, (MTCY24G1.05c), len: 238 aa. Possible transposase for IS1533, similar to is-element proteins e.g. P15026|ISTB_ECOLI istb protein from Escherichia coli (265 aa), FASTA scores: opt: 475, E (): 1.6e-21, (48.0% identity in 148 aa overlap); Z95436|MTY15C10_14 from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 784, E(): 0,(87.4% identity in 135 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible transposase for insertion sequence element IS1533 3290506 887636 Rv2944 Mycobacterium tuberculosis H37Rv Possible transposase for insertion sequence element IS1533 NP_217460.1 3289790 D 83332 CDS NP_217461.1 15610082 887956 complement(3290624..3291325) 1 NC_000962.3 Rv2945c, (MTCY24G1.04), len: 233 aa. Probable lppX,conserved lipoprotein, equivalent to Q9CD80 putative lipoprotein from Mycobacterium leprae (233 aa), FASTA scores: opt: 1165, E(): 2.1e-65, (76.4% identity in 233 aa overlap); and similar to Q9CCP6|ML0557 from Mycobacterium leprae (238 aa), FASTA scores: opt: 338, E(): 7.4e-14,(30.75% identity in 231 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. P71679|LPRG_MYCTU lipoprotein (236 aa), FASTA scores: opt: 342, E(): 4.1e-14,(32.05% identity in 231 aa overlap); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and has in its N-terminal a signal peptide. Belongs to the LPPX/lprafg family of lipoproteins. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved lipoprotein LppX 3291325 lppX 887956 lppX Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppX NP_217461.1 3290624 R 83332 CDS NP_217462.1 15610083 888122 complement(3291503..3296353) 1 NC_000962.3 Rv2946c, (MTCY24G1.03), len: 1616 aa. Probable pks1,polyketide synthase, similar to many e.g. ML035|AL583917|Q9CD81 putative polyketide synthase from Mycobacterium leprae (2103 aa), Fasta scores: opt: 8761,E(): 0, (82.6% identity in 1620 aa overlap); etc. Almost identical in part to G560507|Q50470 PKS002C protein from Mycobacterium tuberculosis (fragment) (950 aa), Fasta scores: opt: 5685, E(): 0, (95.3% identity in 927 aa overlap). Also similar to Mycobacterium tuberculosis polyketide synthases pks7|Rv1661|P94996 (2126 aa) (54.6% identity in 1632 aa); pks12|Rv2048c|O53490 (4151 aa) (58.0% identity in 1606 aa); pks8|rv1662|O65933 (1602 aa) (59.7% identity in 1144 aa). Contains a PS00012 Phosphopantetheine attachment site. Note pks1 has been shown to be involved in the biosynthesis of phthiocerol. pks15/pks1 has been shown to be involved in the biosynthesis of phenolphthiocerol glycolipids.; Probable polyketide synthase Pks1 3296353 pks1 888122 pks1 Mycobacterium tuberculosis H37Rv Probable polyketide synthase Pks1 NP_217462.1 3291503 R 83332 CDS NP_217463.1 15610084 887291 complement(3296350..3297840) 1 NC_000962.3 Rv2947c, (MTCY24G1.02), len: 496 aa. Probable pks15,polyketide synthase. Almost identical to G560508|Q50469 PKS002B protein from Mycobacterium tuberculosis (495 aa),FASTA scores: opt: 3270, E(): 0, (99.6% identity in 496 a a overlap). Similar to Mycobacterium tuberculosis proteins MTCY338.20|RV2931|PPSA_MYCTU ppsA phenolpthiocerol synthesis (1876 aa) (49.9% identity in 465 aa overlap); MTCY24G1.09|RV2940C|P96291 Putative mas, mycocerosic acid synthase (2111 aa) (50.2% identity in 454 aa overlap); and MTCY22H8.03|RV2382C|P71718 hypothetical protein (444 aa) (47.6% identity in 437 aa overlap). Contains PS00606 Beta-ketoacyl synthases active site. Note pks15 has been shown to be involved in the biosynthesis of phthiocerol. pks15/pks1 has been shown to be involved in the biosynthesis of phenolphthiocerol glycolipids.; Probable polyketide synthase Pks15 3297840 pks15 887291 pks15 Mycobacterium tuberculosis H37Rv Probable polyketide synthase Pks15 NP_217463.1 3296350 R 83332 CDS NP_217464.1 15610085 887295 complement(3297837..3299954) 1 NC_000962.3 Rv2948c, (MTCY24G1.01), len: 705 aa. FadD22,p-hydroxybenzoyl-AMP ligase. Highly similar to many e.g. Q9CD82|ML0134 putative acyl-CoA synthetase from Mycobacterium leprae (707 aa), fasta scores: opt: 3554,E(): 6.4e-209, (75.9% identity in 705 aa overlap). Almost identical to G560509|Q50468 PKS002A protein from Mycobacterium tuberculosis (705 aa), fasta scores: opt: 4647, E(): 0, (99.7% identity in 705 aa overlap).; P-hydroxybenzoyl-AMP ligase FadD22 3299954 fadD22 887295 fadD22 Mycobacterium tuberculosis H37Rv P-hydroxybenzoyl-AMP ligase FadD22 NP_217464.1 3297837 R 83332 CDS NP_217465.1 15610086 887206 complement(3299971..3300570) 1 NC_000962.3 Rv2949c, (MTCY349.41), len: 199 aa. Chorismate pyruvate lyase, equivalent to Q9CD83|ML0133 hypothetical protein from Mycobacterium leprae (210 aa), FASTA scores: opt: 797, E(): 7.4e-47, (62.55% identity in 195 aa overlap). Equivalent to AAK47348 from Mycobacterium tuberculosis strain CDC1551 (212 aa) but shorter 13 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Chorismate pyruvate lyase 3300570 887206 Rv2949c Mycobacterium tuberculosis H37Rv Chorismate pyruvate lyase NP_217465.1 3299971 R 83332 CDS NP_217466.3 448824794 887202 complement(3300596..3302455) 1 NC_000962.3 Rv2950c, (MTCY349.40), len: 619 aa. fadD29,fatty-acid-AMP synthetase, similar to various mycobacterial enzymes believed to be involved in polyketide or fatty acid synthesis. Equivalent (but shorter 61 aa) to Q9CD84 from Mycobacterium leprae (680 aa), FASTA scores: opt: 3280,E(): 2.2e-192, (80.15% identity in 620 aa overlap); and highly similar to others from Mycobacterium leprae e.g. Q9Z5K5 probable acyl-CoA synthase (583 aa), FASTA scores: opt: 2358, E(): 3.4e-136, (62.35% identity in 579 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. Q10976|FD26_MYCTU putative fatty-acid--CoA ligase (583 aa), FASTA scores: opt: 2416,E(): 1e-139, (63.15% identity in 581 aa overlap) (N-terminus shorter); etc. Equivalent to AAK47349 from Mycobacterium tuberculosis strain CDC1551 (582 aa) but longer 37 aa.; Fatty-acid-AMP ligase FadD29 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 3302455 fadD29 887202 fadD29 Mycobacterium tuberculosis H37Rv Fatty-acid-AMP ligase FadD29 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_217466.3 3300596 R 83332 CDS NP_217467.1 15610088 887887 complement(3303103..3304248) 1 NC_000962.3 Rv2951c, (MTCY349.39), len: 381 aa. Possible oxidoreductase, equivalent to Q9CD85 putative oxidoreductase from Mycobacterium leprae (382 aa), FASTA scores: opt: 2225, E(): 7.6e-134, (84.8% identity in 382 aa overlap); and similar to O30260 conserved hypothetical protein from Mycobacterium leprae (363 aa), FASTA scores: opt: 652, E(): 6.1e-34, (32.55% identity in 344 aa overlap). Also similar to various oxidoreductases e.g. O29071|AF1196 N5,N10-methylenetetrahydromethanopterin reductase from Archaeoglobus fulgidus (348 aa), FASTA scores: opt: 381, E(): 9.7e-17, (27.7% identity in 354 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 372,E(): 3.5e-16, (30.85% identity in 295 aa overlap); Q9UXP0 putative F420-dependent N5,N10-methylene-tetrahydromethanopterin reductase from Methanolobus tindarius (326 aa), FASTA scores: opt: 343,E(): 2.4e-14, (27.4% identity in 314 aa overlap); etc.; Possible oxidoreductase 3304248 887887 Rv2951c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_217467.1 3303103 R 83332 CDS NP_217468.1 15610089 887311 3304441..3305253 1 NC_000962.3 Rv2952, (MTCY349.38), len: 270 aa. Probable methyltransferase, equivalent to Q9CD86|ML0130 hypothetical protein from Mycobacterium leprae (270 aa), FASTA scores: opt: 1584, E(): 6.1e-99, (83.7% identity in 270 aa overlap). Also highly similar to Q9RMN9|MTF2 putative methyltransferase from Mycobacterium smegmatis (274 aa),FASTA scores: opt: 902, E(): 3.8e-53, (56.35% identity in 252 aa overlap). Also similar to other methyltransferases e.g. Q9ADL4|SORM O-methyltransferase from Polyangium cellulosum (346 aa), FASTA scores: opt: 390, E(): 1.1e-18,(36.25% identity in 251 aa overlap); Q54303|RAPM methyltransferase from Streptomyces hygroscopicus (317 aa),FASTA scores: opt: 315, E(): 1.1e-13, (40.75% identity in 135 aa overlap); etc. Very similar to C-terminal part of Q50584|Rv1523|MTCY19G5.05c hypothetical 37.9 KDA protein from Mycobacterium tuberculosis (358 aa), FASTA score: opt: 965, E(): 2.7e-57, (60.3% identity in 247 aa overlap).; Possible methyltransferase (methylase) 3305253 887311 Rv2952 Mycobacterium tuberculosis H37Rv Possible methyltransferase (methylase) NP_217468.1 3304441 D 83332 CDS NP_217469.1 15610090 887772 3305279..3306535 1 NC_000962.3 Rv2953, (MTCY349.37c), len: 418 aa. Enoyl reductase,equivalent to Q9CD87|ML0129 hypothetical protein from Mycobacterium leprae (418 aa), FASTA scores: opt: 2357,E(): 2.7e-143, (86.6% identity in 418 aa overlap). Also highly similar to Q9X7N5|SC5F2A.12c conserved hypothetical protein from Streptomyces coelicolor (396 aa), FASTA scores: opt: 491, E(): 7e-24, (38.35% identity in 417 aa overlap); and similar to other hypothetical proteins e.g. Q9VG81 CG5167 protein from Drosophila melanogaster (Fruit fly) (431 aa), FASTA scores: opt: 393, E(): 1.4e-17,(26.55% identity in 433 aa overlap); Q9GZE9|F22F7.1 hypothetical protein from Caenorhabditis elegans (426 aa),FASTA scores: opt: 338, E(): 4.6e-14, (27.05% identity in 425 aa overlap); P73855|SLL1601 hypothetical 44.8 KDA protein from Synechocystis sp. (strain PCC 6803) (414 aa),FASTA scores: opt: 565, E(): 1.3e-28, (35.7% identity in 409 aa overlap); etc. Also highly similar to other proteins from Mycobacterium tuberculosis e.g. RV2449C|O53176|MTV008.05C hypothetical 44.4 KDA protein (419 aa), FASTA scores: opt: 1835, E(): 7e-110, (67.55% identity in 419 aa overlap); etc.; Enoyl reductase 3306535 887772 Rv2953 Mycobacterium tuberculosis H37Rv Enoyl reductase NP_217469.1 3305279 D 83332 CDS NP_217470.1 15610091 887214 complement(3306666..3307391) 1 NC_000962.3 Rv2954c, (MTCY349.36), len: 241 aa. Hypothetical unknown protein. Equivalent to AAK47354 from Mycobacterium tuberculosis strain CDC1551 (199 aa) but longer 42 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 3307391 887214 Rv2954c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217470.1 3306666 R 83332 CDS NP_217471.1 15610092 887318 complement(3307580..3308545) 1 NC_000962.3 Rv2955c, (MTCY349.34), len: 321 aa. Conserved protein, similar to others e.g. Q98NV5|MLL9724 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (284 aa),FASTA scores: opt: 231, E(): 6.5e-08, (34.6% identity in 182 aa overlap); Q9AGG2|NLPE1 NLPE1 from Rhizobium etli (249 aa), FASTA scores: opt: 212, E(): 1.1e-06, (27.85% identity in 255 aa overlap); Q9KXY2 hypothetical 31.3 KDA protein from Streptomyces coelicolor(291 aa), FASTA scores: opt: 211, E(): 1.4e-06, (30.9% identity in 249 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3308545 887318 Rv2955c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217471.1 3307580 R 83332 CDS NP_217472.1 15610093 887486 3308668..3309399 1 NC_000962.3 Rv2956, (MTCY349.33c), len: 243 aa. Conserved protein, highly similar to O86299|GSC GSC protein from Mycobacterium avium subsp. silvaticum Mycobacterium avium (240 aa), FASTA scores: opt: 1070, E(): 3.5e-63, (67.5% identity in 240 aa overlap); and O86294|GSC GSC protein from Mycobacterium paratuberculosis (240 aa), FASTA scores: opt: 1070, E(): 3.5e-63, (67.5% identity in 240 aa overlap). Also some similarity with other proteins from other organisms e.g. Q9L727 nodulation protein NOEI from Rhizobium fredii (Sinorhizobium fredii) (241 aa), FASTA scores: opt: 205, E(): 3.5e-06, (27.25% identity in 198 aa overlap); Q9AGG1|LPEA LPEA protein from Rhizobium etli (286 aa), FASTA scores: opt: 201, E(): 7.2e-06, (28.85% identity in 208 aa overlap); P74191|SLL1173 hypothetical 28.0 KDA protein Synechocystis sp. (strain PCC 6803) (244 aa), FASTA scores: opt: 274, E(): 1e-10, (30.65% identity in 225 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P71792|RV1513|MTCY277.35 hypothetical 26.7 KDA protein (243 aa), FASTA scores: opt: 1105, E(): 1.7e-65, (70.05% identity in 237 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3309399 887486 Rv2956 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217472.1 3308668 D 83332 CDS NP_217473.1 15610094 887258 3309470..3310297 1 NC_000962.3 Rv2957, (MTCY349.31c), len: 275 aa. Possible glycosyl transferase ; possibly secreted protein. Highly similar to O88109|GSD|GTFD GSD protein from Mycobacterium avium subsp. silvaticum, Mycobacterium paratuberculosis,and Mycobacterium avium (266 aa), FASTA scores: opt: 1010,E(): 2.5e-62, (68.8% identity in 221 aa overlap). Also some similarity with other proteins and especially glycosyl transferases e.g. Q9AEE4 hypothetical 31.4 KDA protein from Leptospira interrogans (265 aa), FASTA scores: opt: 371,E(): 3.3e-18, (34.43% identity in 212 aa overlap); Q9EXY4 putative glycosyl transferase from Escherichia coli (248 aa), FASTA scores: opt: 339, E(): 5e-16, (32.4% identity in 210 aa overlap); Q9RCC4 glycosyltransferase-like protein from Yersinia pestis (247 aa), FASTA scores: opt: 333, E(): 1.3e-15, (31.8% identity in 217 aa overlap); Q9EXY1 putative glycosyl transferase from Escherichia coli (248 aa), FASTA scores: opt: 328, E(): 2.9e-15, (31.9% identity in 210 aa overlap); etc. Equivalent to AAK47357 from Mycobacterium tuberculosis strain CDC1551 (256 aa) but longer 19 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible glycosyl transferase 3310297 887258 Rv2957 Mycobacterium tuberculosis H37Rv Possible glycosyl transferase NP_217473.1 3309470 D 83332 CDS NP_217474.1 15610095 887816 complement(3310714..3312000) 1 NC_000962.3 Rv2958c, (MTCY349.30), len: 428 aa. Possible glycosyl transferase (see citation below), highly similar to Q9CD88|ML0128 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2116,E(): 5.8e-126, (75.05% identity in 417 aa overlap); and Q9CD91|ML0125 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2104,E(): 3.3e-125, (74.65% identity in 418 aa overlap). Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3 putative glycosyltransferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 317, E(): 1.9e-12,(31.0% identity in 297 aa overlap); Q9S1V2 putative glycosyl transferase from Streptomyces coelicolor (407 aa),FASTA scores: opt: 264, E(): 4.1e-09, (27.2% identity in 342 aa overlap); P72650|CRTX|SLR1125 zeaxanthin glucosyl transferase from Synechocystis sp. strain PCC 6803 (419 aa), FASTA scores: opt: 251, E(): 2.8e-08, (26.8% identity in 295 aa overlap); etc. Very similar to P95130|MTCY349.25 from Mycobacterium tuberculosis (449 aa), FASTA score: opt: 2215, E(): 3.3e-132, (77.25% identity in 422 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible glycosyl transferase 3312000 887816 Rv2958c Mycobacterium tuberculosis H37Rv Possible glycosyl transferase NP_217474.1 3310714 R 83332 CDS NP_217475.1 15610096 887862 complement(3312101..3312838) 1 NC_000962.3 Rv2959c, (MTCY349.29), len: 245 aa. Possible methyltransferase, highly similar to Q9CD89|ML0127 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1183,E(): 3.9e-69, (76.1% identity in 226 aa overlap). Also some similarity with other methyltransferases and other proteins e.g. Q51079 putative methyl transferase from Nocardia lactamdurans (236 aa), FASTA scores: opt: 156, E(): 0.0086,(23.25% identity in 159 aa overlap); Q98ID5 cephalosporin hydroxylase from Rhizobium loti (Mesorhizobium loti) (217 aa), FASTA scores: opt: 275, E(): 1.7e-10, (29.65% identity in 199 aa overlap); etc. And also similar to P72897 hypothetical 27.8 KDA protein from Mycobacterium tuberculosis (249 aa), FASTA scores: opt: 292, E(): 1.5e-11, (31.25% identity in 208 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible methyltransferase (methylase) 3312838 887862 Rv2959c Mycobacterium tuberculosis H37Rv Possible methyltransferase (methylase) NP_217475.1 3312101 R 83332 CDS NP_217476.1 15610097 887224 complement(3312953..3313201) 1 NC_000962.3 Rv2960c, (MT3036, MTCY349.28), len: 82 aa. Hypothetical unknown protein, equivalent to AAK47362 from Mycobacterium tuberculosis strain CDC1551 (116 aa) but shorter 34 aa. Shortened version of MTCY349.28 avoiding overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 3313201 887224 Rv2960c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217476.1 3312953 R 83332 CDS NP_217477.1 15610098 887316 3313283..3313672 1 NC_000962.3 Rv2961, (MTCY349.26c), len: 129 aa. Probable transposase, highly similar to C-terminus of O50414|Rv3387|MTV004.45 putative transposase from Mycobacterium tuberculosis (225 aa), FASTA scores: opt: 605, E(): 7.2e-34, (66.65% identity in 129 aa overlap); and similar to others e.g. CAC47401 putative partial transposase for ISRM17 protein from Rhizobium meliloti (Sinorhizobium meliloti) (174 aa), FASTA scores: opt: 183,E(): 2.6e-05, (30.25% identity in 129 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase 3313672 887316 Rv2961 Mycobacterium tuberculosis H37Rv Probable transposase NP_217477.1 3313283 D 83332 CDS NP_217478.1 15610099 887892 complement(3313773..3315122) 1 NC_000962.3 Rv2962c, (MTCY349.25), len: 449 aa. Possible glycosyl transferase (see citation below), highly similar or identical to Mycobacterium tuberculosis proteins G560522 U0002JA, G560521 U0002H, G560522 U0002JA, G560519 U0002KA. Equivalent (but longer 21 aa) to Q9CD91 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2229, E(): 1.3e-133, (77.45% identity in 426 aa overlap); and highly similar to Q9CD88 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2129, E(): 2.7e-127, (74.35% identity in 425 aa overlap); and others from Mycobacterium leprae. Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3|DRA0329 putative glycosyl transferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 340,E(): 5.5e-14, (31.2% identity in 330 aa overlap); P72650 zeaxanthin glucosyl transferase from Synechocystis sp. (strain PCC 6803) (419 aa), FASTA scores: opt: 244, E(): 6.6e-08, (26.2% identity in 294 aa overlap); etc. Also highly similar to P95134 hypothetical 46.8 KDA protein from Mycobacterium tuberculosis (428 aa), FASTA scores: opt: 2215, E(): 9.6e-133, (77.25% identity in 422 aa overlap).; Possible glycosyl transferase 3315122 887892 Rv2962c Mycobacterium tuberculosis H37Rv Possible glycosyl transferase NP_217478.1 3313773 R 83332 CDS NP_217479.1 15610100 887263 3315236..3316456 1 NC_000962.3 Rv2963, (MTCY349.24c), len: 406 aa. Probable integral membrane protein.; Probable integral membrane protein 3316456 887263 Rv2963 Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_217479.1 3315236 D 83332 CDS NP_217480.1 15610101 887338 3316529..3317461 1 NC_000962.3 Rv2964, (MTCY349.23c), len: 310 aa. Probable purU,formyltetrahydrofolate deformylase, highly similar to others e.g. Q9RWT1|DR0584 formyltetrahydrofolate deformylase from Deinococcus radiodurans (298 aa), FASTA scores: opt: 1005, E(): 4.9e-52, (52.25% identity in 297 aa overlap); Q9K7U4 formyltetrahydrofolate deformylase from Bacillus halodurans (289 aa), FASTA scores: opt: 982, E(): 1.1e-50, (51.8% identity in 280 aa overlap); Q55135|PURU_SYNY3|SLL0070 formyltetrahydrofolate deformylase from Synechocystis sp. strain PCC 6803 (284 aa), FASTA scores: opt: 839, E(): 2.9e-42, (48.2% identity in 280 aa overlap); etc.; Probable formyltetrahydrofolate deformylase PurU (formyl-FH(4) hydrolase) 3317461 purU 887338 purU Mycobacterium tuberculosis H37Rv Probable formyltetrahydrofolate deformylase PurU (formyl-FH(4) hydrolase) NP_217480.1 3316529 D 83332 CDS NP_217481.1 15610102 888423 complement(3318330..3318815) 1 NC_000962.3 Rv2965c, (MTCY349.22), len: 161 aa. Probable kdtB (alternate gene name: coaD), phosphopantetheine adenylyltransferase, equivalent to O69466|COAD_MYCLE phosphopantetheine adenylyltransferase from Mycobacterium leprae (160 aa), FASTA scores: opt: 881, E(): 2.5e-54,(84.1% identity in 157 aa overlap). Also highly similar to others e.g. Q9ZBR1|COAD_STRCO from Streptomyces coelicolor (159 aa), FASTA scores: opt: 575, E(): 5.8e-33, (54.1% identity in 159 aa overlap); Q9WZK0|COAD_THEMA from Thermotoga maritima (161 aa), FASTA scores: opt: 509, E(): 2.4e-28, (50.0% identity in 154 aa overlap); P23875|COAD_ECOLICOAD|KDTB|B3634|Z5058|ECS4509 from Escherichia coli strain O157:H7 and K12 (159 aa), FASTA scores: opt: 459, E(): 7.3e-25, (45.15% identity in 155 aa overlap); etc. Belongs to the CoaD family.; Probable phosphopantetheine adenylyltransferase KdtB (pantetheine-phosphate adenylyltransferase) (PPAT) (dephospho-CoA pyrophosphorylase) 3318815 kdtB 888423 kdtB Mycobacterium tuberculosis H37Rv Probable phosphopantetheine adenylyltransferase KdtB (pantetheine-phosphate adenylyltransferase) (PPAT) (dephospho-CoA pyrophosphorylase) NP_217481.1 3318330 R 83332 CDS NP_217482.1 15610103 888469 complement(3318901..3319467) 1 NC_000962.3 Rv2966c, (MTCY349.21), len: 188 aa. Possible methyltransferase, equivalent (but shorter 36 aa) to O69465|MLCB1243.09 hypothetical 23.0 KDA protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 872, E(): 9.1e-50, (74.2% identity in 182 aa overlap). Also similar to others e.g. Q9ZBR2|SC7A1.11 putative methylase from Streptomyces coelicolor (195 aa), FASTA scores: opt: 510,E(): 3.7e-26, (47.5% identity in 179 aa overlap); Q9F842 hypothetical methyltransferase (fragment) from Mycobacterium smegmatis (80 aa), FASTA scores: opt: 386,E(): 2.5e-18, (75.0% identity in 80 aa overlap); P10120|YHHF_ECOLI|YHHFZ|B3465 putative methylase from Escherichia colistrain K12 (198 aa), FASTA scores: opt: 319, E(): 1.1e-13, (35.5% identity in 183 aa overlap); etc. Contains PS00092 N-6 Adenine-specific DNA methylases signature.; Possible methyltransferase (methylase) 3319467 888469 Rv2966c Mycobacterium tuberculosis H37Rv Possible methyltransferase (methylase) NP_217482.1 3318901 R 83332 CDS NP_217483.1 15610104 887299 complement(3319663..3323046) 1 NC_000962.3 Rv2967c, (MTCY349.20), len: 1127 aa. Probable pca,pyruvate carboxylase (ala-rich protein), equivalent to Q9F843|PYC pyruvate carboxylase from Mycobacterium smegmatis (1127 aa), FASTA scores: opt: 6232, E(): 0,(83.3% identity in 1127 aa overlap). Also highly similar to others e.g. Q9RK64|SCF11.26c pyruvate carboxylase from Streptomyces coelicolor (1124 aa), FASTA scores: opt: 5526,E(): 0, (74.65% identity in 1125 aa overlap); O54587|PYC pyruvate carboxylase from Corynebacterium glutamicum (Brevibacterium flavum) (1140 aa), FASTA scores: opt: 4811,E(): 0, (64.5% identity in 1132 aa overlap); Q9DDT1 pyruvate carboxylase from Brachydanio rerio (Zebrafish) (1180 aa), FASTA scores: opt: 3133, E(): 1.1e-171, (47.8% identity in 1142 aa overlap); etc. Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2, PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site, and PS00188 Biotin-requiring enzymes attachment site.; Probable pyruvate carboxylase Pca (pyruvic carboxylase) 3323046 pca 887299 pca Mycobacterium tuberculosis H37Rv Probable pyruvate carboxylase Pca (pyruvic carboxylase) NP_217483.1 3319663 R 83332 CDS NP_217484.1 15610105 888476 complement(3323071..3323703) 1 NC_000962.3 Rv2968c, (MTCY349.19), len: 210 aa. Probable conserved integral membrane protein, equivalent to O69464 putative integral membrane protein from Mycobacterium leprae (214 aa), FASTA scores: opt: 1060, E(): 1.4e-58,(71.95% identity in 214 aa overlap). Also highly similar to others e.g. Q9F844 hypothetical integral membrane protein from Mycobacterium smegmatis (187 aa), FASTA scores: opt: 883, E(): 1.2e-47, (62.8% identity in 190 aa overlap); Q9KXP3 putative integral membrane protein from Streptomyces coelicolor (240 aa), FASTA scores: opt: 503, E(): 4.6e-24,(38.0% identity in 192 aa overlap); etc.; Probable conserved integral membrane protein 3323703 888476 Rv2968c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217484.1 3323071 R 83332 CDS NP_217485.1 15610106 888481 complement(3323709..3324476) 1 NC_000962.3 Rv2969c, (MTCY349.18), len: 255 aa. Possible conserved membrane or exported protein, equivalent to Q9CBS4|ML1667 possible conserved membrane protein from Mycobacterium leprae (264 aa), FASTA scores: opt: 1101,E(): 9.9e-68, (65.9% identity in 258 aa overlap); and highly similar to O69463 putative transmembrane protein from Mycobacterium leprae (258 aa), FASTA scores: opt: 1097, E(): 1.8e-67, (65.5% identity in 258 aa overlap). C-terminus also highly similar to Q9KK65|996A160 exported protein (fragment) from Mycobacterium avium (85 aa), FASTA scores: opt: 418, E(): 2e-21, (72.95% identity in 85 aa overlap). Also weakly similar to membrane or exported proteins e.g. Q9S2U7|SC4G6.04c putative integral membrane protein from Streptomyces coelicolor (275 aa), FASTA scores: opt: 312, E(): 7.6e-14, (28.25% identity in 230 aa overlap); Q9XAB6|SCC22.22C putative secreted protein from Streptomyces coelicolor (255 aa), FASTA scores: opt: 181,E(): 6.4e-05, (27.0% identity in 226 aa overlap); etc. Also some similarity with P72001|PKNE_MYCTU from Mycobacterium tuberculosis (566 aa), FASTA scores: opt: 264, E(): 2.3e-10, (30.5% identity in 177 aa overlap).; Possible conserved membrane or secreted protein 3324476 888481 Rv2969c Mycobacterium tuberculosis H37Rv Possible conserved membrane or secreted protein NP_217485.1 3323709 R 83332 CDS NP_217486.1 15610107 887194 complement(3324573..3325703) 1 NC_000962.3 Rv2970c, (MTCY349.17), len: 376 aa. Probable lipN,lipase/esterase, similar to others e.g. Q9AA37|CC0771 putative esterase from Caulobacter crescentus (380 aa),FASTA scores: opt: 822, E(): 8e-46, (42.15% identity in 318 aa overlap); Q9XDR4 esterase HDE from petroleum-degrading bacterium HD-1 (317 aa), FASTA scores: opt: 738, E(): 2e-40, (48.85% identity in 262 aa overlap); O52270 lipase from Pseudomonas sp. (strain B11-1) (308 aa), FASTA scores: opt: 683, E(): 7.3e-37, (41.3% identity in 288 aa overlap); etc. Also similar to P71668 hypothetical 34.1 KDA protein from Mycobacterium tuberculosis (320 aa), FASTA scores: opt: 715, E(): 6.3e-39, (42.3% identity in 298 aa overlap). Equivalent to AAK47374 from Mycobacterium tuberculosis strain CDC1551 (309 aa) but longer 67 aa.; Probable lipase/esterase LipN 3325703 lipN 887194 lipN Mycobacterium tuberculosis H37Rv Probable lipase/esterase LipN NP_217486.1 3324573 R 83332 CDS YP_177681.1 57117040 3205038 3325934..3326104 1 NC_000962.3 Rv2970A, len: 56 aa. Conserved hypothetical protein,similar to C-terminal part of several oxidoreductases e.g. Rv2971|Z83018|MTCY349_22 from Mycobacterium tuberculosis (282 aa), FASTA scores: opt: 158, E(): 3.6e-06, (45.0% identity in 60 aa overlap). May represent a gene fragment.; hypothetical protein 3326104 3205038 Rv2970A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177681.1 3325934 D 83332 CDS NP_217487.1 15610108 887275 3326101..3326949 1 NC_000962.3 Rv2971, (MTCY349.16c), len: 282 aa. Probable oxidoreductase, possibly aldo/keto reductase, equivalent to O69462 putative oxidoreductase from Mycobacterium leprae (282 aa), FASTA scores: opt: 1495, E(): 4.9e-93, (82.35% identity in 272 aa overlap). Also similar to others e.g. Q9KYM9|SC9H11.10C oxidoreductase from Streptomyces coelicolor (276 aa), FASTA scores: opt: 849, E(): 1.2e-49,(51.7% identity in 267 aa overlap); Q9ZBW7|SC4B5.01C putative oxidoreductase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 847, E(): 1.7e-49, (49.1% identity in 271 aa overlap); Q46857|YQHE_ECOLI|YQHE|B3012 hypothetical oxidoreductase from Escherichia coli strain K12 (275 aa), FASTA scores: opt: 827, E(): 3.7e-48, (47.45% identity in 276 aa overlap); etc. Contains PS00063 Aldo/keto reductase family putative active site signature; and PS00062 Aldo/keto reductase family signature 2.; Probable oxidoreductase 3326949 887275 Rv2971 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_217487.1 3326101 D 83332 CDS NP_217488.1 15610109 887191 complement(3327023..3327736) 1 NC_000962.3 Rv2972c, (MTCY349.15), len: 237 aa. Possible conserved membrane or exported protein, equivalent (but longer 52 aa) to O69461|MLCB1243.02 hypothetical 20.5 KDA protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 581, E(): 8.2e-32, (55.75% identity in 174 aa overlap). Also similar to membrane or exported proteins e.g. Q9F2P3|SCE41.16C putative lipoprotein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 498,E(): 4.1e-26, (44.08% identity in 186 aa overlap); Q99QB5|SCP1.323C putative secreted protein from Streptomyces coelicolor (219 aa), FASTA scores: opt: 329,E(): 8.5e-15, (36.35% identity in 176 aa overlap); Q9ACQ1|SCP1.267 putative secreted protein from Streptomyces coelicolor (219 aa), FASTA scores: opt: 286, E(): 6.6e-12,(32.03% identity in 231 aa overlap); etc.; Possible conserved membrane or exported protein 3327736 887191 Rv2972c Mycobacterium tuberculosis H37Rv Possible conserved membrane or exported protein NP_217488.1 3327023 R 83332 CDS NP_217489.1 15610110 887439 complement(3327733..3329946) 1 NC_000962.3 Rv2973c, (MTCY349.14), len: 737 aa. Probable recG,ATP-dependent DNA helicase (see citation below), equivalent to O69460|RECG_MYCLE ATP-dependent DNA helicase from Mycobacterium leprae (743 aa), FASTA scores: opt: 3846,E(): 0, (79.3% identity in 744 aa overlap). Also highly similar to others e.g. Q9ZBR3|SC7A1.10 putative ATP-dependent DNA helicase from Streptomyces coelicolor (742 aa), FASTA scores: opt: 1249, E(): 1.1e-67, (46.2% identity in 758 aa overlap); Q9PGE8 ATP-dependent DNA helicase from Xylella fastidiosa (718 aa), FASTA scores: opt: 1174, E(): 3.5e-63, (42.1% identity in 539 aa overlap); P24230|RECG_ECOLI|RECG|B3652 from Escherichia coli strain K12 (693 aa), FASTA scores: opt: 457, E(): 7.3e-22, (35.2% identity in 733 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the helicase family, RECG subfamily.; Probable ATP-dependent DNA helicase RecG 3329946 recG 887439 recG Mycobacterium tuberculosis H37Rv Probable ATP-dependent DNA helicase RecG NP_217489.1 3327733 R 83332 CDS NP_217490.1 15610111 887383 complement(3329949..3331361) 1 NC_000962.3 Rv2974c, (MTCY349.13), len: 470 aa. Conserved hypothetical ala-rich protein, highly similar to others e.g. C-terminus of Q9ZBR4|SC7A1.09 hypothetical 59.5 KDA protein from Streptomyces coelicolor (589 aa), FASTA scores: opt: 774, E(): 1.3e-36, (41.0% identity in 495 aa overlap); Q9K9Z6|BH2498 hypothetical protein from Bacillus halodurans (557 aa), FASTA scores: opt: 268, E(): 8e-08,(27.7% identity in 502 aa overlap) (N-terminus longer 76 aa); Q9X293 conserved hypothetical protein from Thermotoga maritima (497 aa), FASTA scores: opt: 265, E(): 1.1e-07,(24.9% identity in 470 aa overlap) (N-terminus longer 43 aa); etc. Also some similarity with P47609|Y369_MYCGE|MG369 hypothetical protein from Mycoplasma genitalium (557 aa),FASTA scores: opt: 154, E(): 0.25, (20.25% identity in 489 aa overlap); this, and following ORF, are similar to Y369_MYCGE but no cosmid sequence error was identified.; Conserved hypothetical alanine rich protein 3331361 887383 Rv2974c Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine rich protein NP_217490.1 3329949 R 83332 CDS NP_217491.1 15610112 887512 complement(3331358..3331612) 1 NC_000962.3 Rv2975c, (MTCY349.12), len: 84 aa. Conserved hypothetical protein, similar to N-terminus of others e.g. Q9ZBR4|SC7A1.09 hypothetical 59.5 KDA protein from Streptomyces coelicolor (589 aa), FASTA scores: opt: 141,E(): 0.0019, (41.25% identity in 80 aa overlap); Q98R49|MYPU_1610 hypothetical protein from Mycoplasma pulmonis (545 aa), FASTA scores: opt: 127, E(): 0.023,(48.0% identity in 50 aa overlap); Q9K9Z6|BH2498 hypothetical protein from Bacillus halodurans (557 aa),FASTA scores: opt: 126, E(): 0.028, (34.55% identity in 81 aa overlap); etc. Also some similarity with N-terminus of P47609|Y369_MYCGE|MG369 hypothetical protein from Mycoplasma genitalium (557 aa), FASTA scores: opt: 108,E(): 0.7, (36.75% identity in 49 aa overlap); this, and preceding ORF, are similar to Y369_MYCGE and YLOV protein but no cosmid sequence error was identified.; hypothetical protein 3331612 887512 Rv2975c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217491.1 3331358 R 83332 CDS NP_217492.1 15610113 887410 complement(3332071..3332754) 1 NC_000962.3 Rv2976c, (MTCY349.11), len: 227 aa. Probable ung,uracil-DNA glycosylase (see citation below), equivalent to Q9CBS3 uracil-DNA glycosylase from Mycobacterium leprae (227 aa), FASTA scores: opt: 1394, E(): 8.8e-85, (88.1% identity in 227 aa overlap). Also highly similar to others e.g. Q9EX12 from Streptomyces coelicolor (225 aa), FASTA scores: opt: 1134, E(): 1.3e-67, (72.75% identity in 224 aa overlap); Q9K682|UNG_BACHD from Bacillus halodurans (224 aa), FASTA scores: opt: 652, E(): 8.9e-36, (45.5% identity in 222 aa overlap); P39615|UNG_BACSU from Bacillus subtilis (225 aa), FASTA scores: opt: 625, E(): 5.4e-34, (45.5% identity in 222 aa overlap); etc. Belongs to the uracil-DNA glycosylase family.; Probable uracil-DNA glycosylase Ung (UDG) 3332754 ung 887410 ung Mycobacterium tuberculosis H37Rv Probable uracil-DNA glycosylase Ung (UDG) NP_217492.1 3332071 R 83332 CDS NP_217493.1 15610114 888342 complement(3332787..3333788) 1 NC_000962.3 Rv2977c, (MTCY349.10), len: 333 aa. Possible thiL,thiamin-monophosphate kinase, equivalent to Q9CBS2 probable thiamine-monophosphate kinase from Mycobacterium leprae (325 aa), FASTA scores: opt: 1738, E(): 4.5e-98, (80.9% identity in 314 aa overlap). Also highly similar to others e.g. Q9ZBR7|SC7A1.06 putative thiamine monphosphate kinase from Streptomyces coelicolor (322 aa), FASTA scores: opt: 959, E(): 7.8e-51, (51.1% identity in 319 aa overlap); O05514|THIL_BACSU thiamine-monophosphate kinase from Bacillus subtilis (325 aa), FASTA scores: opt: 476, E(): 1.5e-21, (35.15% identity in 273 aa overlap); P77785|THIL_ECOLI|THIL|B0417 thiamine-monophosphate kinase from Escherichia coli strain K12 (325 aa), FASTA scores: opt: 418, E(): 5e-18, (36.9% identity in 282 aa overlap); etc. Belongs to the thiamine-monophosphate kinase family. Note that the start, as given, is in IS1538.; Probable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase) 3333788 thiL 888342 thiL Mycobacterium tuberculosis H37Rv Probable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase) NP_217493.1 3332787 R 83332 CDS NP_217494.1 15610115 887390 complement(3333785..3335164) 1 NC_000962.3 Rv2978c, (MTCY349.09), len: 459 aa. Probable transposase for IS1538, very similar to several other putative transposases from Mycobacterium tuberculosis e.g. YX16_MYCTU|Q10809 (460 aa), FASTA scores: opt: 2613, E(): 0, (83.0% identity in 458 aa overlap); etc. Low level matches to other tranposases.; Probable transposase 3335164 887390 Rv2978c Mycobacterium tuberculosis H37Rv Probable transposase NP_217494.1 3333785 R 83332 CDS NP_217495.1 15610116 888325 complement(3335164..3335748) 1 NC_000962.3 Rv2979c, (MTCY349.08), len: 194 aa. Probable resolvase for IS1538, with low level matches to transposon resolvases; highly similar from aa 101 to YX1C_MYCTU|Q10831 from Mycobacterium tuberculosis (295 aa), FASTA scores: opt: 809, E(): 0, (69.1% identity in 194 aa overlap). Contains PS00397 Site-specific recombinases active site,and possible helix-turn-helix motiv at aa 2-23.; Probable resolvase 3335748 888325 Rv2979c Mycobacterium tuberculosis H37Rv Probable resolvase NP_217495.1 3335164 R 83332 CDS NP_217496.1 15610117 887506 3335960..3336505 1 NC_000962.3 Rv2980, (MTCY349.07c), len: 181 aa. Possible conserved secreted protein, equivalent to Q9CBS1 possible secreted protein from Mycobacterium leprae (191 aa), FASTA scores: opt: 794, E(): 2.3e-40, (67.25% identity in 177 aa overlap). Also some weak similarity with other hypothetical proteins or secreted proteins e.g. C-terminus of Q98F98|MLL3872 MLL3872 protein from Rhizobium loti (Mesorhizobium loti) (575 aa), FASTA scores: opt: 148, E(): 0.16, (28.35% identity in 194 aa overlap); Q9L0W9|SCH22A.13C putative secreted protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 114,E(): 7.5, (40.0% identity in 80 aa overlap); etc. Equivalent to AAK47385 from Mycobacterium tuberculosis strain CDC1551 (214 aa) but shorter 33 aa. Has hydrophobic stretch near N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved secreted protein 3336505 887506 Rv2980 Mycobacterium tuberculosis H37Rv Possible conserved secreted protein NP_217496.1 3335960 D 83332 CDS NP_217497.1 15610118 888415 complement(3336796..3337917) 1 NC_000962.3 Rv2981c, (MTCY349.06), len: 373 aa. Probable ddlA (alternate gene name: ddl), D-alanine--D-alanine ligase a (see citation below), equivalent to Q9CBS0|Q9CBS0 D-alanine-D-alanine ligase a from Mycobacterium leprae (384 aa), FASTA scores: opt: 2001, E(): 2.4e-115, (81.75% identity in 367 aa overlap); and Q9ZGN0|DDL_MYCSM D-alanine--D-alanine ligase from Mycobacterium smegmatis (373 aa), FASTA scores: opt: 1934, E(): 3.1e-111, (77.95% identity in 372 aa overlap). Also highly similar to others e.g. Q9ZBR9|DDL_STRCO from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1187, E(): 2.2e-65, (52.0% identity in 379 aa overlap); P15051|DDLA_SALTY from Salmonella typhimurium and Salmonella typhi (363 aa), FASTA scores: opt: 946, E(): 1.3e-50, (44.5% identity in 364 aa overlap); P23844|DDLA_ECOLI|DDLA|B0381|Z0477|ECS0431 from Escherichia coli strain O157:H7 and K12 (364 aa), FASTA scores: opt: 938, E(): 3.9e-50, (43.55% identity in 363 aa overlap); etc. Contains PS00843 D-alanine--D-alanine ligase signature 1. Belongs to the D-alanine--D-alanine ligase family.; Probable D-alanine--D-alanine ligase DdlA (D-alanylalanine synthetase) (D-ala-D-ala ligase) 3337917 ddlA 888415 ddlA Mycobacterium tuberculosis H37Rv Probable D-alanine--D-alanine ligase DdlA (D-alanylalanine synthetase) (D-ala-D-ala ligase) NP_217497.1 3336796 R 83332 CDS NP_217498.1 15610119 887864 complement(3337995..3338999) 1 NC_000962.3 Rv2982c, (MTCY349.05), len: 334 aa. Probable gpdA2 (alternate gene name: gpsA), glycerol-3-phosphate dehydrogenase [NAD(P)+], equivalent to Q9CBR9|GPDA_MYCLE glycerol-3-phosphate dehydrogenase [NAD(P)+] from Mycobacterium leprae (349 aa), FASTA scores: opt: 1686,E(): 1.7e-95, (77.95% identity in 349 aa overlap). Also highly similar to others e.g. Q9ZBS0|GPDA_STRCO from Streptomyces coelicolor (336 aa), FASTA scores: opt: 1165,E(): 9.8e-64, (56.25% identity in 327 aa overlap); P46919|GPDA_BACSU from Bacillus subtilis (345 aa), FASTA scores: opt: 872, E(): 7.5e-46, (44.9% identity in 325 aa overlap); P37606|GPDA_ECOLI|GPSA|B3608|Z5035|ECS4486. from Escherichia coli strain O157:H7 and K12 (339 aa), FASTA scores: opt: 799, E(): 2.1e-41, (42.9% identity in 331 aa overlap); etc. Also highly similar to O53761|GPD2_MYCTU probable glycerol-3-phosphate dehydrogenase from Mycobacterium tuberculosis (341 aa), FASTA scores: opt: 740, E(): 8.4e-38, (40.35% identity in 322 aa overlap). Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.; Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA2 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) 3338999 gpdA2 887864 gpdA2 Mycobacterium tuberculosis H37Rv Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA2 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) NP_217498.1 3337995 R 83332 CDS NP_217499.1 15610120 888161 3339118..3339762 1 NC_000962.3 Rv2983, (MTCY349.04c), len: 214 aa. Conserved hypothetical ala-rich protein, equivalent to O33128|ML1680|MLCB637.37c hypothetical 22.0 KDA protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1080, E(): 9e-61, (79.05% identity in 215 aa overlap). Also similar to other hypothetical proteins e.g. Q9ZBS2|SC7A1.01C from Streptomyces coelicolor (212 aa),FASTA scores: opt: 420, E(): 2.9e-19, (43.5% identity in 207 aa overlap); O26710|MTH613 from Methanothermobacter thermautotrophicus (223 aa), FASTA scores: opt: 193, E(): 5.8e-05, (30.0% identity in 190 aa overlap); Q9RKG8|SCE46.21 from Streptomyces coelicolor (210 aa),FASTA scores: opt: 139, E(): 0.14, (27.65% identity in 206 aa overlap); etc.; Conserved hypothetical alanine rich protein 3339762 888161 Rv2983 Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine rich protein NP_217499.1 3339118 D 83332 CDS NP_217500.1 15610121 887482 3339854..3342082 1 NC_000962.3 Rv2984, (MTCY349.03c), len: 742 aa. Ppk1,polyphosphate kinase (See Sureka et al., 2007), equivalent to O33127|PPK_MYCLE polyphosphate kinase from Mycobacterium leprae (739 aa), FASTA scores: opt: 4264, E(): 0, (87.85% identity in 742 aa overlap). Also highly similar to others e.g. Q9KZV6|PPK_STRCO from Streptomyces coelicolor (746 aa), FASTA scores: opt: 1979, E(): 2.6e-117, (59.9% identity in 701 aa overlap); Q9KD27|PPK_BACHD from Bacillus halodurans (705 aa), FASTA scores: opt: 1319, E(): 1.4e-75,(45.55% identity in 674 aa overlap); Q9PAC7|PPK_XYLFA from Xylella fastidiosa (698 aa), FASTA scores: opt: 1300, E(): 2.2e-74, (43.3% identity in 693 aa overlap); etc. Belongs to the polyphosphate kinase family.; Polyphosphate kinase PPK (polyphosphoric acid kinase) (ATP-polyphosphate phosphotransferase) 3342082 ppk1 887482 ppk1 Mycobacterium tuberculosis H37Rv Polyphosphate kinase PPK (polyphosphoric acid kinase) (ATP-polyphosphate phosphotransferase) NP_217500.1 3339854 D 83332 CDS NP_217501.1 15610122 888165 3342165..3343118 1 NC_000962.3 Rv2985, (MTCY349.02c), len: 317 aa. Possible mutT1,long MutT protein (hydrolase) (see citation below), highly similar to O33126|MLCB637.35 hypothetical 34.5 KDA protein from Mycobacterium leprae (312 aa), FASTA scores: opt: 1514, E(): 5.1e-91, (71.85% identity in 316 aa overlap); and Q9CBR8|ML1682 hypothetical protein from Mycobacterium leprae (311 aa), FASTA scores: opt: 1510, E(): 9.2e-91,(71.5% identity in 316 aa overlap). Also similar to Q50195|L222-ORF6|ML2698 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 231, E(): 1.1e-07, (36.7% identity in 128 aa overlap). Also similar to shorter mutt proteins and related hypothetical protein e.g. Q9EUS6 hypothetical 16.6 KDA protein from Streptomyces griseus subsp. griseus (152 aa), FASTA scores: opt: 380,E(): 1.7e-17, (50.75% identity in 130 aa overlap); Q9KZV8|SCD84.10C putative mutt-like protein from Streptomyces coelicolor (142 aa), FASTA scores: opt: 376,E(): 2.9e-17, (46.1% identity in 128 aa overlap); P96590|mutt mutt protein from Bacillus subtilis (149 aa),FASTA scores: opt: 180, E(): 0.00017, (35.25% identity in 122 aa overlap); etc. Also similar to O05437 hypothetical 27.1 KDA protein from Mycobacterium tuberculosis (248 aa),FASTA scores: opt: 224, E(): 3.2e-07, (34.03% identity in 144 aa overlap). Contains PS00893 mutT domain signature. Seems to belong to the mutt/NUDIX family protein.; Possible hydrolase MutT1 3343118 mutT1 888165 mutT1 Mycobacterium tuberculosis H37Rv Possible hydrolase MutT1 NP_217501.1 3342165 D 83332 CDS NP_217502.1 15610123 888166 complement(3343176..3343820) 1 NC_000962.3 Rv2986c, (MTCY349.01), len: 214 aa. hupB (alternate gene names: hup, hlp, lbp21), DNA-binding protein HU homolog (resembles fusion between HU and histone) (see Pethe et al., 2002), equivalent to others from Mycobacteria e.g. Q9XB18|DBH_MYCBO from Mycobacterium bovis (205 aa),FASTA scores: opt: 1050, E(): 5.6e-45, (95.35% identity in 214 aa overlap); Q9ZHC5|DBH_MYCSM from Mycobacterium smegmatis (208 aa), FASTA scores: opt: 1035, E(): 3.1e-44,(80.2% identity in 217 aa overlap); and O33125|DBH_MYCLE from Mycobacterium leprae (200 aa), FASTA scores: opt: 914,E(): 2.7e-38, (80.1% identity in 216 aa overlap). Also highly similar to others from other organisms e.g. O86537|DBH2_STRCO from Streptomyces coelicolor (218 aa),FASTA scores: opt: 569, E(): 2.6e-21, (51.35% identity in 220 aa overlap); P08821|DBH1_BACSU from Bacillus subtilis (92 aa), FASTA scores: opt: 280, E(): 2.5e-07, (45.05% identity in 91 aa overlap) (C-terminus shorter); etc. Contains PS00045 Bacterial histone-like DNA-binding proteins signature. Belongs to the bacterial histone-like protein family. Note that its C-terminal domain is very rich in lysine and alanine.; DNA-binding protein HU homolog HupB (histone-like protein) (HLP) (21-kDa laminin-2-binding protein) 3343820 hupB 888166 hupB Mycobacterium tuberculosis H37Rv DNA-binding protein HU homolog HupB (histone-like protein) (HLP) (21-kDa laminin-2-binding protein) NP_217502.1 3343176 R 83332 CDS NP_217503.1 15610124 888225 complement(3344033..3344629) 1 NC_000962.3 Rv2987c, (MTV012.01c), len: 198 aa. Probable leuD,3-isopropylmalate dehydratase, small subunit, equivalent to O33124|LEUD_MYCLE 3-isopropylmalate dehydratase small subunit from Mycobacterium leprae (198 aa), FASTA scores: opt: 1155, E(): 4.2e-72, (87.75% identity in 196 aa overlap). Also highly similar to many e.g. O86535|LEUD_STRCO from Streptomyces coelicolor (197 aa),FASTA scores: opt: 765, E(): 2.6e-45, (59.0% identity in 195 aa overlap); P04787|LEUD_SALTY from Salmonella typhimurium (201 aa), FASTA scores: opt: 528, E(): 5.2e-29,(45.05% identity in 191 aa overlap); P30126|LEUD_ECOLI|LEUD|B0071 from Escherichia coli strain K12 (201 aa), FASTA scores: opt: 498, E(): 6e-27, (43.45% identity in 191 aa overlap); etc.; Probable 3-isopropylmalate dehydratase (small subunit) LeuD (isopropylmalate isomerase) (alpha-IPM isomerase) (IPMI) 3344629 leuD 888225 leuD Mycobacterium tuberculosis H37Rv Probable 3-isopropylmalate dehydratase (small subunit) LeuD (isopropylmalate isomerase) (alpha-IPM isomerase) (IPMI) NP_217503.1 3344033 R 83332 CDS NP_217504.1 15610125 887875 complement(3344654..3346075) 1 NC_000962.3 Rv2988c, (MTV012.02c), len: 473 aa. Probable leuC,3-isopropylmalate dehydratase, large subunit, equivalent to O33123|LEU2_MYCLE 3-isopropylmalate dehydratase small subunit from Mycobacterium leprae (476 aa), FASTA scores: opt: 2818, E(): 1.3e-171, (88.75% identity in 471 aa overlap). Also highly similar to many e.g. Q44427|LEU2_ACTTI from Actinoplanes teichomyceticus (485 aa), FASTA scores: opt: 1958, E(): 6.5e-117, (71.0% identity in 479 aa overlap); P55251|LEU2_RHIPU from Rhizomucor pusillus (755 aa), FASTA scores: opt: 1937, E(): 1.9e-115, (61.25% identity in 467 aa overlap) (C-terminus longer); P30127|LEU2_ECOLI|LEUC|B0072 from Escherichia coli strain K12 (465 aa), FASTA scores: opt: 1896, E(): 5.5e-113, (61.6% identity in 456 aa overlap); etc. Contains PS00450 Aconitase family signature. Belongs to the aconitase/IPM isomerase family.; Probable 3-isopropylmalate dehydratase (large subunit) LeuC (isopropylmalate isomerase) (alpha-IPM isomerase) (IPMI) 3346075 leuC 887875 leuC Mycobacterium tuberculosis H37Rv Probable 3-isopropylmalate dehydratase (large subunit) LeuC (isopropylmalate isomerase) (alpha-IPM isomerase) (IPMI) NP_217504.1 3344654 R 83332 CDS NP_217505.1 15610126 887723 3346147..3346848 1 NC_000962.3 Rv2989, (MTV012.03), len: 233 aa. Probable transcriptional regulator (ala-rich protein), highly similar to O86533|SC1C2.33c putative transcriptional regulator from Streptomyces coelicolor (238 aa), FASTA scores: opt: 711, E(): 2.3e-38, (53.05% identity in 230 aa overlap); and similar to others e.g. Q9KND6 putative transcriptional regulator from Vibrio cholerae (244 aa),FASTA scores: opt: 232, E(): 1.2e-07, (29.75% identity in 232 aa overlap); Q9R9U0|SRPS efflux pump regulator from Pseudomonas putida (259 aa), FASTA scores: opt: 224, E(): 4.1e-07, (28.35% identity in 247 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. O06806|Rv1773c|MTCY28.39 hypothetical 26.6 KDA protein (248 aa), FASTA scores: opt: 239, E(): 4.4e-08, (29.85% identity in 231 aa overlap); P71977|RV1719|MTCY04C12.04 hypothetical 27.9 KDA protein (259 aa), FASTA scores: opt: 215, E(): 1.6e-06, (31.85% identity in 223 aa overlap); etc. Equivalent to AAK47396 from Mycobacterium tuberculosis strain CDC1551 (267 aa) but shorter 34 aa. Contains possible helix-turn-helix motif at aa 25-46 (Score 1005,+2.61 SD).; Probable transcriptional regulatory protein 3346848 887723 Rv2989 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein NP_217505.1 3346147 D 83332 CDS NP_217506.1 15610127 887176 complement(3346859..3347719) 1 NC_000962.3 Rv2990c, (MTV012.04c), len: 286 aa. Hypothetical unknown protein.; Hypothetical protein 3347719 887176 Rv2990c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217506.1 3346859 R 83332 CDS NP_217507.1 15610128 887792 3347982..3348473 1 NC_000962.3 Rv2991, (MTV012.05), len: 163 aa. Conserved protein,similar to others e.g. Q9K3X7|2SCG61.39. hypothetical 17.6 KDA protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 266, E(): 2.1e-11, (34.85% identity in 155 aa overlap); Q9CNX3|PM0299 hypothetical protein from Pasteurella multocida (171 aa), FASTA scores: opt: 175,E(): 5.1e-05, (31.3% identity in 131 aa overlap); Q9KZI9|SCG8A.10 conserved hypothetical protein from Streptomyces coelicolor (142 aa), FASTA scores: opt: 163,E(): 0.00031, (32.4% identity in 108 aa overlap); etc. Also some similarity to O06553|MTCI65.22|Rv1155 hypothetical protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 127, E(): 0.1, (32.9% identity in 73 aa overlap); and to several proteins of similar size that confer resistance to 5-Nitroimidazole antibiotics in Bacteroides.; hypothetical protein 3348473 887792 Rv2991 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217507.1 3347982 D 83332 CDS YP_177915.1 57117041 887850 complement(3348805..3350277) 1 NC_000962.3 Rv2992c, (MTV012.06c), len: 490 aa. GltS (alternate gene name: gltX), glutamyl-tRNA synthase, equivalent to O33120|SYE_MYCLE glutamyl-tRNA synthetase from Mycobacterium leprae (502 aa), FASTA scores: opt: 2660,E(): 2.3e-163, (81.35% identity in 488 aa overlap). Also highly similar to others e.g. O86528|SYE_STRCO from Streptomyces coelicolor (494 aa), FASTA scores: opt: 1777,E(): 1.4e-106, (57.45% identity in 484 aa overlap); P22250|SYE_BACSU from Bacillus subtilis (483 aa), FASTA scores: opt: 1099, E(): 5.4e-63, (38.45% identity in 489 aa overlap); O51345|SYE_BORBU|GLTX|BB0372 from Borrelia burgdorferi (Lyme disease spirochete) (490 aa), FASTA scores: opt: 1009, E(): 3.3e-57, (34.85% identity in 491 aa overlap); etc. Belongs to class-I aminoacyl-tRNA synthetase family.; Glutamyl-tRNA synthetase GltS (glutamate--tRNA ligase) (glutamyl-tRNA synthase) (GLURS) 3350277 gltS 887850 gltS Mycobacterium tuberculosis H37Rv Glutamyl-tRNA synthetase GltS (glutamate--tRNA ligase) (glutamyl-tRNA synthase) (GLURS) YP_177915.1 3348805 R 83332 CDS NP_217509.3 448824795 887867 complement(3350274..3350993) 1 NC_000962.3 Rv2993c, (MTV012.07c), len: 239 aa. Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, equivalent to O33119|ML1689|MLCB637.28 possible 2-hydroxyhepta-2,4-diene- 1,7-dioate isomerase from Mycobacterium leprae (242 aa), FASTA scores: opt: 1427,E(): 4.4e-86, (85.9% identity in 241 aa overlap). Also similar to others e.g. Q9LBE3|DR1609 from Deinococcus radiodurans (250 aa), FASTA scores: opt: 723, E(): 5.5e-40,(49.05% identity in 216 aa overlap); O27551|MTH1507 from Methanothermobacter thermautotrophicus (260 aa), FASTA scores: opt: 708, E(): 5.4e-39, (52.1% identity in 213 aa overlap); Q9HQR6|VNG1037G|HPCE from Halobacterium sp. (strain NRC-1) (244 aa), FASTA scores: opt: 590, E(): 2.7e-31, (43.65% identity in 220 aa overlap); etc. Start chosen by homology, but ORF could continue upstream.; Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) 3350993 887867 Rv2993c Mycobacterium tuberculosis H37Rv Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) NP_217509.3 3350274 R 83332 CDS NP_217510.1 15610131 888200 3351269..3352606 1 NC_000962.3 Rv2994, (MTV012.08), len: 445 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug. C-terminal part highly similar to O33118|MLCB637.27c hypothetical 14.7 KDA protein (probable pseudogene product) from Mycobacterium leprae (134 aa), FASTA scores: opt: 483,E(): 2.7e-21, (60.9% identity in 138 aa overlap). Also similar to various transporters e.g. Q9I5C8|PA0811 probable MFS transporter from Pseudomonas aeruginosa (415 aa), FASTA scores: opt: 289, E(): 1.3e-09, (26.05% identity in 399 aa overlap); O30210|AF0025 cyanate transport protein from Archaeoglobus fulgidus (393 aa), FASTA scores: opt: 281,E(): 3.7e-09, (24.05% identity in 399 aa overlap); Q9RI35|SCJ12.25C putative nitrate/nitrite transporter from Streptomyces coelicolor (412 aa), FASTA scores: opt: 264,E(): 3.8e-08, (24.95% identity in 409 aa overlap); Q9A5N5|CC2412 major facilitator family transporter from Caulobacter crescentus (405 aa), FASTA scores: opt: 263,E(): 4.3e-08, (27.55% identity in 399 aa overlap); etc. First start taken; similarity to P21191|NORA_STAAU quinolone resistance protein from Staphylococcus aureus (388 aa) suggests alternative start at 7319 but then no positively charged aa before first transmembrane segment.; Probable conserved integral membrane protein 3352606 888200 Rv2994 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217510.1 3351269 D 83332 CDS NP_217511.1 15610132 888182 complement(3352458..3353468) 1 NC_000962.3 Rv2995c, (MTV012.09), len: 336 aa. Probable leuB,3-isopropylmalate dehydrogenase, identical except a single bp to P94929|LEU3_MYCBO 3-isopropylmalate dehydrogenase from Mycobacterium bovis (336 aa) (see citation below),FASTA scores: opt: 2168, E(): 5.1e-132, (99.7% identity in 336 aa overlap); and equivalent to O33117|LEU3_MYCLE 3-isopropylmalate dehydrogenase from Mycobacterium leprae (336 aa), FASTA scores: opt: 1864, E(): 1.8e-112, (83.95% identity in 336 aa overlap). Also highly similar to others e.g. P94631|LEU3_CORGL from Corynebacterium glutamicum (340 aa), FASTA scores: opt: 1526, E(): 1e-90, (69.9% identity in 339 aa overlap); O86504 from Streptomyces coelicolor (347 aa), FASTA scores: opt: 1470, E(): 4.2e-87, (67.85% identity in 339 aa overlap); Q9UZ05|PAB2424 from Pyrococcus abyssi (354 aa), FASTA scores: opt: 998, E(): 1e-56, (50.0% identity in 322 aa overlap); etc. Note that also shows high similarity with many tartrate dehydrogenases. Belongs to the isocitrate and isopropylmalate dehydrogenases family.; Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH) 3353468 leuB 888182 leuB Mycobacterium tuberculosis H37Rv Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH) NP_217511.1 3352458 R 83332 CDS YP_177916.1 57117042 887154 complement(3353483..3355069) 1 NC_000962.3 Rv2996c, (MTV012.10), len: 528 aa. Probable serA1,D-3-phosphoglycerate dehydrogenase, equivalent to SERA_MYCLE D-3-phosphoglycerate dehydrogenase from Mycobacterium leprae (528 aa), FASTA scores: opt: 2974,E(): 1.9e-166, (89.6% identity in 528 aa overlap). Also highly similar to many e.g. Q9Z564 from Streptomyces coelicolor (529 aa), FASTA scores: opt: 1879, E(): 2.1e-102, (57.6% identity in 526 aa overlap); O29445|SERA_ARCFU from Archaeoglobus fulgidus (527 aa),FASTA scores: opt: 1252, E(): 9.6e-66, (41.3% identity in 530 aa overlap); P35136|SERA_BACSU from Bacillus subtilis (525 aa), FASTA scores: opt: 1172, E(): 4.5e-61, (37.9% identity in 528 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00065 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature, and PS00670 D-isomer specific 2-hydroxyacid dehydrogenases signature 2. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenases family. Note that previously known as serA.; Probable D-3-phosphoglycerate dehydrogenase SerA1 (PGDH) 3355069 serA1 887154 serA1 Mycobacterium tuberculosis H37Rv Probable D-3-phosphoglycerate dehydrogenase SerA1 (PGDH) YP_177916.1 3353483 R 83332 CDS NP_217513.1 15610134 888637 3355099..3356541 1 NC_000962.3 Rv2997, (MTV012.11), len: 480 aa. Possible ala-rich dehydrogenase, similar to others dehydrogenases and hypothetical proteins e.g. Q9EYI5 putative dehydrogenase from Streptomyces nogalater (472 aa), FASTA scores: opt: 1131, E(): 1.7e-61, (41.0% identity in 471 aa overlap); Q9ZBG4|SC9B5.16 putative dehydrogenase from Streptomyces coelicolor (472 aa), FASTA scores: opt: 1064, E(): 2e-57,(39.05% identity in 471 aa overlap); Q98BS8 probable dehydrogenase from Rhizobium loti (Mesorhizobium loti) (524 aa), FASTA scores: opt: 196, E(): 0.00021, (25.1% identity in 526 aa overlap); etc. Shows strong similarity throughout its length to O06826|MTCY493.22c|Rv1432 hypothetical 50.5 KDA protein from Mycobacterium tuberculosis (473 aa), FASTA scores: opt: 1220, E(): 6.1e-67, (42.35% identity in 465 aa overlap).; Possible alanine rich dehydrogenase 3356541 888637 Rv2997 Mycobacterium tuberculosis H37Rv Possible alanine rich dehydrogenase NP_217513.1 3355099 D 83332 CDS NP_217514.1 15610135 888646 3356815..3357276 1 NC_000962.3 Rv2998, (MTV012.12), len: 153 aa. Hypothetical unknown protein. Note that equivalent to AAK47405 Hypothetical 19.4 kDa protein from Mycobacterium tuberculosis strain CDC1551 (186 aa) but sequence differs in N-terminus.; Hypothetical protein 3357276 888646 Rv2998 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217514.1 3356815 D 83332 CDS YP_177682.1 57117043 3205090 complement(3357225..3357428) 1 NC_000962.3 Rv2998A, len: 67 aa. Probable conserved hypothetical protein, (possibly gene fragment), highly similar to central part of two-component sensor proteins e.g. O07777|Rv0601c|MTCY19H5.21 two component sensor (fragment) from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 212, E(): 3.7e-09, (58.2% identity in 67 aa overlap); Q9L2B6|SC8F4.08 probable two-component sensor kinase from Streptomyces coelicolor (478 aa), FASTA scores: opt: 193,E(): 2.6e-07, (47.05% identity in 68 aa overlap); etc.; hypothetical protein 3357428 3205090 Rv2998A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177682.1 3357225 R 83332 CDS NP_217515.1 15610136 887357 3357602..3358567 1 NC_000962.3 Rv2999, (MTV012.13), len: 321 aa. Probable lppY,conserved lipoprotein, highly similar to O07774|LPQO|Rv0604|MTCY19H5.18c putative lipoprotein from Mycobacterium tuberculosis (316 aa), FASTA scores: opt: 1153, E(): 5e-62, (53.2% identity in 312 aa overlap); and showing similarity with AAK80743|CAC2799 uncharacterized conserved protein similar to LPPY/LPQO of Mycobacterium tuberculosis from Clostridium acetobutylicum (152 aa),FASTA scores: opt: 165, E(): 0.0077, (26.08% identity in 138 aa overlap); and Q9F2T1|SCD65.01c putative lipoprotein (fragment) from Streptomyces coelicolor (146 aa), FASTA scores: opt: 126, E(): 1.6, (% identity in aa overlap). Equivalent to AAK47407 from Mycobacterium tuberculosis strain CDC1551 (329 aa) but shorter 8 aa. Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LppY 3358567 lppY 887357 lppY Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppY NP_217515.1 3357602 D 83332 CDS NP_217516.1 15610137 887271 3358612..3359271 1 NC_000962.3 Rv3000, (MTV012.14), len: 219 aa. Possible conserved transmembrane protein, similar to various membrane proteins e.g. P77307|YBBM_ECOLI|B0491 hypothetical 28.2 KDA protein (potential integral membrane protein) from Escherichia coli strain K12 (259 aa), FASTA scores: opt: 292, E(): 3.1e-11,(30.25% identity in 218 aa overlap); N-terminus of Q9BJF3 putative ABC transporter (fragment) from Sterkiella histriomuscorum (1319 aa), FASTA scores: opt: 274, E(): 1.3e-09, (39.6% identity in 101 aa overlap); Q9C9W0|T23K23.21 putative ABC transporter from Arabidopsis thaliana (Mouse-ear cress) (263 aa), FASTA scores: opt: 258, E(): 4.4e-09, (30.1% identity in 196 aa overlap); P74369|YG47_SYNY3|SLR1647 hypothetical 28.1 KDA protein (potential integral membrane protein) from Synechocystis sp. strain PCC 6803 (259 aa), FASTA scores: opt: 257, E(): 5.1e-09, (37.75% identity in 98 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible conserved transmembrane protein 3359271 887271 Rv3000 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_217516.1 3358612 D 83332 CDS NP_217517.3 448824796 887483 complement(3359585..3360586) 1 NC_000962.3 Rv3001c, (MT3081, MTV012.15c), len: 333 aa. Probable ilvC, ketol-acid reductoisomerase, equivalent or highly similar to others e.g. Q59500|ILVC_MYCAV from Mycobacterium avium (333 aa), FASTA scores: opt: 1977, E(): 3.2e-113,(87.7% identity in 333 aa overlap); O33114|ILVC_MYCLE from Mycobacterium leprae (333 aa), FASTA scores: opt: 1924,E(): 5.3e-110, (86.5% identity in 333 aa overlap); Q9Z565|ILVC_STRCO|SC8D9.26 from Streptomyces coelicolor (332 aa), FASTA scores: opt: 1494, E(): 8.3e-84, (67.5% identity in 326 aa overlap); Q59818|ILVC_STRAW from Streptomyces avermitilis (333 aa) FASTA scores: opt: 1487,E(): 2.2e-83, (66.8% identity in 326 aa overlap); etc. Belongs to the KETOL-acid reductoisomerases family.; Probable KETOL-acid reductoisomerase IlvC (acetohydroxy-acid isomeroreductase) (alpha-keto-beta-hydroxylacil reductoisomerase) 3360586 ilvC 887483 ilvC Mycobacterium tuberculosis H37Rv Probable KETOL-acid reductoisomerase IlvC (acetohydroxy-acid isomeroreductase) (alpha-keto-beta-hydroxylacil reductoisomerase) NP_217517.3 3359585 R 83332 CDS NP_217518.1 15610139 887226 complement(3360624..3361130) 1 NC_000962.3 Rv3002c, (MT3082, MTV012.16c), len: 168 aa. Probable ilvN (alternate gene name: ilvH), acetolactate synthase,small subunit, equivalent or highly similar to others e.g. O33113|ILVH_MYCLE|MLCB637.21 from Mycobacterium leprae (169 aa), FASTA scores: opt: 843, E(): 5.1e-47, (83.5% identity in 164 aa overlap); Q59499|ILVH_MYCAV|ILVN from Mycobacterium avium (167 aa), FASTA scores: opt: 798, E(): 3.7e-44, (81.05% identity in 169 aa overlap); Q9Z566|ILVN from Streptomyces coelicolor (174 aa), FASTA scores: opt: 678, E(): 1.7e-36, (64.8% identity in 159 aa overlap); etc. Belongs to the acetolactate synthase small subunit family.; Probable acetolactate synthase (small subunit) IlvN (acetohydroxy-acid synthase) (AHAS) (ALS) 3361130 ilvN 887226 ilvN Mycobacterium tuberculosis H37Rv Probable acetolactate synthase (small subunit) IlvN (acetohydroxy-acid synthase) (AHAS) (ALS) NP_217518.1 3360624 R 83332 CDS YP_177917.1 57117044 887286 complement(3361130..3362986) 1 NC_000962.3 Rv3003c, (MT3083, MTV012.17c), len: 618 aa. ilvB1,acetolactate synthase, large subunit, equivalent or highly similar to others e.g. O33112|ILVB_MYCLE|MLCB637.20|ML1696 from Mycobacterium leprae (625 aa), FASTA scores: opt: 3653, E(): 5.4e-208, (87.1% identity in 627 aa overlap); Q59498|ILVB_MYCAV from Mycobacterium avium (621 aa), FASTA scores: opt: 3473, E(): 2.3e-197, (84.7% identity in 614 aa overlap); P42463|ILVB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (626 aa), FASTA scores: opt: 2754,E(): 5.9e-155, (65.8% identity in 589 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature. Cofactor: thiamine pyrophosphate, and magnesium (by similarity). Note that previously known as ilvB.; Acetolactate synthase (large subunit) IlvB1 (acetohydroxy-acid synthase) 3362986 ilvB1 887286 ilvB1 Mycobacterium tuberculosis H37Rv Acetolactate synthase (large subunit) IlvB1 (acetohydroxy-acid synthase) YP_177917.1 3361130 R 83332 CDS NP_217520.1 15610141 887891 3363348..3363686 1 NC_000962.3 Rv3004, (MT3084.1, MTV012.18), len: 112 aa. Cfp6,low molecular weight protein antigen 6 (CFP-6) (See Bhaskar et al., 2000). Weak homology with Q9RKZ5|SC6D7.02 putative membrane protein from Streptomyces coelicolor (156 aa),FASTA scores: opt: 109, E(): 0.78, (39.4% identity in 122 aa overlap). Caution: the initiator methionine may be further upstream making the sequence a precursor. Predicted to be an outer membrane protein (See Song et al., 2008).; Low molecular weight protein antigen 6 (CFP-6) 3363686 cfp6 887891 cfp6 Mycobacterium tuberculosis H37Rv Low molecular weight protein antigen 6 (CFP-6) NP_217520.1 3363348 D 83332 CDS NP_217521.1 15610142 887305 complement(3363693..3364532) 1 NC_000962.3 Rv3005c, (MTV012.19c), len: 279 aa. Conserved hypothetical protein, equivalent to O33110|MLCB637.18|ML1698 hypothetical 29.5 KDA protein from Mycobacterium leprae (277 aa), FASTA scores: opt: 1245,E(): 1.2e-65, (70.5% identity in 278 aa overlap). Also similar, but longer approximately 100 aa in N-terminus, to other hypothetical proteins, few membrane proteins, e.g. Q9RKN9|SCC75A.35 putative membrane protein from Streptomyces coelicolor (180 aa), FASTA scores: opt: 326,E(): 3.9e-12, (44.2% identity in 138 aa overlap); P96694|YDFP|AB001488 hypothetical protein from Bacillus subtilis (129 aa), FASTA scores: opt:273, E(): 3.7e-09,(33.1% identity in 130 aa overlap); Q9KKT1|VCA1019 hypothetical protein from Vibrio cholerae (148 aa), FASTA scores: opt: 258, E(): 3.1e-08, (34.9% identity in 126 aa overlap); etc.; hypothetical protein 3364532 887305 Rv3005c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217521.1 3363693 R 83332 CDS NP_217522.1 15610143 887308 3364709..3365830 1 NC_000962.3 Rv3006, (MTV012.20), len: 373 aa. Probable lppZ,conserved lipoprotein, equivalent to O33109|MLCB637.17C|ML1699 putative lipoprotein from M. leprae (372 aa), FASTA scores: opt: 2211, E(): 4.3e-100,(87.1% identity in 373 aa overlap). Shows also similarity (in part) with Q9Z571|SC8D9.20c putative oxidoreductase from Streptomyces coelicolor (447 aa), FASTA scores: opt: 185, E(): 0.051, (31.6% identity in 300 aa overlap); Q9Z9R3|BH2090 glucose dehydrogenase-B from Bacillus halodurans (371 aa), FASTA scores: opt: 206, E(): 0.0043,(28.3% identity in 205 aa overlap); and other glucose dehydrogenases B. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, followed by a proline-rich domain.; Probable conserved lipoprotein LppZ 3365830 lppZ 887308 lppZ Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LppZ NP_217522.1 3364709 D 83332 CDS NP_217523.1 15610144 888938 complement(3365836..3366450) 1 NC_000962.3 Rv3007c, (MTV012.21c), len: 204 aa. Possible oxidoreductase, similar to Q9EWU5|3SC5B7.04c putative oxidoreductase from Streptomyces coelicolor (162 aa), FASTA scores: opt: 376, E(): 1.5e-18, (41.35% identity in 150 aa overlap); Q9K416|SCG22.29c putative flavin-dependent reductase protein from Streptomyces coelicolor (169 aa),FASTA scores: opt: 246, E(): 1e-09, (34.1% identity in 135 aa overlap); and some similarity to coupling proteins of 4-hydroxyphenylacetic hydroxylase/monooxygenase e.g. Q9HWT6|HPAC|PA4092 Pseudomonas aeruginosa (170 aa), FASTA score: opt: 214; O68232|HPAC Photorhabdus luminescens (Xenorhabdus luminescens) (172 aa), FASTA score: opt: 198; Q9RPU2|HPAC Salmonella dublin (170 aa), FASTA score: opt: 197; etc. Equivalent to AAK47416 from Mycobacterium tuberculosis strain CDC1551 (236 aa) but shorter 32 aa. Start chosen by similarity.; Possible oxidoreductase 3366450 888938 Rv3007c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_217523.1 3365836 R 83332 CDS NP_217524.1 15610145 888530 3366644..3367267 1 NC_000962.3 Rv3008, (MTV012.22), len: 207 aa (start uncertain). Hypothetical unknown protein.; Hypothetical protein 3367267 888530 Rv3008 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217524.1 3366644 D 83332 CDS NP_217525.1 15610146 888919 complement(3367264..3368793) 1 NC_000962.3 Rv3009c, (MT3089, MTV012.23c), len: 509 aa. Probable gatB, Glu- tRNA-Gln amidotransferase, subunit B ,equivalent to O33107|GATB_MYCLE|MLCB637_15 glutamyl-tRNA(GLN) amidotransferase from Mycobacterium leprae (509 aa), FASTA scores: opt: 2973, E(): 2.9e-173,(88.4% identity in 509 aa overlap). Also highly similar to other Glu- tRNA-Gln amidotransferases e.g. Q9Z578|GATB|SC8D9.13 from Streptomyces coelicolor (504 aa),FASTA scores: opt: 2264, E(): 3.6e-130, (66.0% identity in 495 aa overlap); P74215|GATB_SYNY3|SLL1435 from Synechocystis sp. strain PCC 6803 (519 aa), FASTA scores: opt: 1289, E(): 6.7e-71, (42.0% identity in 485 aa overlap); Q9X100|GATB_THEMA|TM1273 glutamyl-tRNA(GLN) amidotransferase from Thermotoga maritima (482 aa), FASTA scores: opt: 1165, E(): 2.2e-63, (40.05% identity in 487 aa overlap); etc. For more information about function, see citation below. Similar to many members of the pet112 family. Belongs to the GatB family.; Probable glutamyl-tRNA(GLN) amidotransferase (subunit B) GatB (Glu-ADT subunit B) 3368793 gatB 888919 gatB Mycobacterium tuberculosis H37Rv Probable glutamyl-tRNA(GLN) amidotransferase (subunit B) GatB (Glu-ADT subunit B) NP_217525.1 3367264 R 83332 CDS NP_217526.1 15610147 888509 complement(3368823..3369854) 1 NC_000962.3 Rv3010c, (MTV012.24c), len: 343 aa. Probable pfkA,phosphofructokinase, equivalent to O33106|K6PF_MYCLE|MLCB637.14 6-phosphofructokinase from Mycobacterium leprae (343 aa), FASTA scores: opt: 2099,E(): 4.1e-122, (90.4% identity in 343 aa overlap). Also highly similar to others e.g. Q9FC99|K6P3_STRCO from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1329,E(): 1.1e-74, (58.9% identity in 338 aa overlap); Q9L1L8|K6P2_STRCO|PFKA2|PFK2|SC6A11.02 6-phosphofructokinase 2 from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1303, E(): 4.5e-73, (56.7% identity in 342 aa overlap); Q9KH71|PFP PPI-dependent phosphofructokinase from Dictyoglomus thermophilum (346 aa), FASTA scores: opt: 893, E(): 8.4e-48, (41.85% identity in 344 aa overlap); etc. Contains PS00433 Phosphofructokinase signature. Belongs to the phosphofructokinase family.; Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase) 3369854 pfkA 888509 pfkA Mycobacterium tuberculosis H37Rv Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase) NP_217526.1 3368823 R 83332 CDS NP_217527.2 448824797 887262 complement(3369950..3371434) 1 NC_000962.3 Rv3011c, (MT3091, MTV012.25c), len: 494 aa. Probable gatA, Glu-tRNA-Gln amidotransferase, subunit A , equivalent to O33105|GATA|ML1702|MLCB637.13 glutamyl-tRNA(GLN) amidotransferase from Mycobacterium leprae (497 aa), FASTA scores: opt: 2839, E(): 3.5e-161, (88.8% identity in 492 aa overlap). Also highly similar to other Glu-tRNA-Gln amidotransferases e.g. Q9Z580|GATA_STRCO from Streptomyces coelicolor (497 aa), FASTA scores: opt: 2231, E(): 4.5e-125, (70.3% identity in 486 aa overlap); P73558|GATA_SYNY3|SLR0877 from Synechocystis sp. strain PCC 6803 (483 aa), FASTA scores: opt: 1593, E(): 3.3e-87,(55.85% identity in 487 aa overlap); O06491|GATA_BACSU glutamyl-tRNA(GLN) amidotransferase from Bacillus subtilis (485 aa), FASTA scores: opt: 1389, E(): 4.3e-75, (51.7% identity in 468 aa overlap); etc. For more information about function, see citation below. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the amidase family. Nucleotide position 3370177 in the genome sequence has been corrected, T:G resulting in M420L.; Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A) 3371434 gatA 887262 gatA Mycobacterium tuberculosis H37Rv Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A) NP_217527.2 3369950 R 83332 CDS NP_217528.1 15610149 887335 complement(3371431..3371730) 1 NC_000962.3 Rv3012c, (MT3092, MTV012.26c), len: 99 aa. Probable gatC, Glu-tRNA-Gln amidotransferase, subunit C, equivalent to O33104|GATC_MYCLE|MLCB637.12 glutamyl-tRNA(GLN) amidotransferase from Mycobacterium leprae (99 aa), FASTA scores: opt: 483, E(): 3.1e-25, (74.75% identity in 99 aa overlap). Also highly similar to other Glu-tRNA-Gln amidotransferases e.g. Q9Z581|GATC_STRCO|SC8D9.10 from Streptomyces coelicolor (98 aa), FASTA scores: opt: 298,E(): 4e-13, (53.7% identity in 95 aa overlap); O06492|GATC_BACSU from B. subtilis (96 aa), FASTA scores: opt: 222, E(): 3.7e-08, (43.15% identity in 95 aa overlap); Q9KF29|BH0665 from Bacillus halodurans (96 aa), FASTA scores: opt: 211, E(): 1.9e-07, (41.05% identity in 95 aa overlap); etc. For more information about function, see citation below. Belongs to the GatC family.; Probable glutamyl-tRNA(GLN) amidotransferase (subunit C) GatC (Glu-ADT subunit C) 3371730 gatC 887335 gatC Mycobacterium tuberculosis H37Rv Probable glutamyl-tRNA(GLN) amidotransferase (subunit C) GatC (Glu-ADT subunit C) NP_217528.1 3371431 R 83332 CDS NP_217529.1 15610150 888642 3371815..3372471 1 NC_000962.3 Rv3013, (MTV012.27), len: 218 aa. Conserved protein,equivalent to O33103|MLCB637_11c hypothetical 24.4 KDA protein from Mycobacterium leprae (230 aa), FASTA scores: opt: 1188, E(): 2.6e-67, (83.95% identity in 218 aa overlap). Equivalent to AAK47422 from Mycobacterium tuberculosis strain CDC1551 (240 aa) but shorter 22 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3372471 888642 Rv3013 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217529.1 3371815 D 83332 CDS NP_217530.1 15610151 887354 complement(3372545..3374620) 1 NC_000962.3 Rv3014c, (MT3094, MTV012.28c), len: 691 aa. ligA (alternate gene name: lig), DNA ligase NAD-dependent (see citation below), equivalent to O33102|DNLJ_MYCLE|LIGA|LIG|ML1705|MLCB637.10 DNA ligase from Mycobacterium leprae (694 aa), FASTA scores: opt: 3844, E(): 0, (84.7% identity in 687 aa overlap). Also highly similar to many prokaryotic and eukaryotic ligases e.g. Q9Z585|LIGA|SC8D9.06 from Streptomyces coelicolor (735 aa), FASTA scores: opt: 2002, E(): 4e-113, (59.4% identity in 714 aa overlap); P49421|DNLJ_RHOMR|LIGA|LIG from Rhodothermus marinus (712 aa), FASTA scores: opt: 1835,E(): 4.6e-103, (45.55% identity in 685 aa overlap); P15042|DNLJ_ECOLI|LIGA|LIG|DNAL|PDEC|lop|B2411 from Escherichia coli strain K12 (671 aa), FASTA scores: opt: 1696, E(): 1.1e-94, (43.8% identity in 680 aa overlap); etc. Belongs to the NAD-dependent DNA ligase family.; DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]) 3374620 ligA 887354 ligA Mycobacterium tuberculosis H37Rv DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]) NP_217530.1 3372545 R 83332 CDS NP_217531.1 15610152 887181 complement(3374651..3375664) 1 NC_000962.3 Rv3015c, (MTV012.29c), len: 337 aa. Conserved hypothetical protein, equivalent to Q9CBR6|ML1706 hypothetical protein from Mycobacterium leprae (337 aa),FASTA scores: opt: 1703, E(): 3.1e-92, (78.05% identity in 337 aa overlap); and (but longer 47 aa) O33101|MLCB637.09 hypothetical 30.0 KDA protein from Mycobacterium leprae (290 aa), FASTA scores: opt: 1564, E(): 2.4e-78, (78.6% identity in 290 aa overlap). Also similar to Q9Z586|SC8D9.05 hypothetical 35.0 KDA protein from Streptomyces coelicolor (331 aa), FASTA scores: opt: 774,E(): 4.7e-38, (43.4% identity in 334 aa overlap); and showing similarity with other proteins e.g. Q39586|METE_CHLRE 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase from Chlamydomonas reinhardtii (814 aa),FASTA scores: opt: 162, E(): 0.048, (27.05% identity in 355 aa overlap).; hypothetical protein 3375664 887181 Rv3015c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217531.1 3374651 R 83332 CDS NP_217532.1 15610153 888557 3375758..3376387 1 NC_000962.3 Rv3016, (MTV012.30), len: 209 aa. Probable lpqA,lipoprotein. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable lipoprotein LpqA 3376387 lpqA 888557 lpqA Mycobacterium tuberculosis H37Rv Probable lipoprotein LpqA NP_217532.1 3375758 D 83332 CDS NP_217533.1 15610154 888508 complement(3376490..3376852) 1 NC_000962.3 Rv3017c, (MT3097, MTV012.31c), len: 120 aa. EsxQ,ESAT-6 like protein (see citation below), possibly secreted protein, very similar to AAK47433|MT3104 putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis strain CDC1551 (96 aa), FASTA scores: opt: 315, E(): 1.2e-14, (65.7% identity in 70 aa overlap); Rv3019c|O53266|MTV012.33c putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 315, E(): 1.2e-14, (65.7% identity in 70 aa overlap) and Rv0288|O53693|CFP7|MT0301|MTV035.16 10 KDA antigen CFP7 (low molecular weight protein antigen 7) (CFP-7) from Mycobacterium tuberculosis (95 aa), FASTA scores: opt: 303, E(): 7.4e-14, (66.2% identity in 68 aa overlap). An alternative start site exists at 3376801. Belongs to the ESAT6 family. Note previously known as TB12.9.; ESAT-6 like protein EsxQ (TB12.9) (ESAT-6 like protein 8) 3376852 esxQ 888508 esxQ Mycobacterium tuberculosis H37Rv ESAT-6 like protein EsxQ (TB12.9) (ESAT-6 like protein 8) NP_217533.1 3376490 R 83332 CDS YP_177918.1 57117045 888940 complement(3376939..3378243) 1 NC_000962.3 Rv3018c, (MTV012.32c), len: 434 aa. PPE46, Member of PPE family but lacks Gly, Ala rich repeats at C-terminal domain, closest to MTCY261.19. See citation below. Also very similar to following ORF MTV012.35c. Nearly identical in parts to Mycobacterium tuberculosis protein erroneously described as dihydrofolate reductase (X59271|MTFOLA_1) P31500|DYR_MYCTU (214 aa), FASTA scores: opt: 972, E(): 4.4e-42, (80.0% identity in 195 aa overlap); and Z97559|MTCY261_19 from Mycobacterium tuberculosis cosmid (473 aa), FASTA scores: opt: 806, E(): 0; (38.8% identity in 479 aa overlap); and O53268|MTV012.35c from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 1714, E(): 3.3e-79, (78.3% identity in 355 aa overlap).; PPE family protein PPE46 3378243 PPE46 888940 PPE46 Mycobacterium tuberculosis H37Rv PPE family protein PPE46 YP_177918.1 3376939 R 83332 CDS YP_007411731.1 448817858 3205087 complement(3378329..3378415) 1 NC_000962.3 Rv3018A, len: 28 aa. PE27A, Member of Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), most similar to Rv0285 (102 aa), FASTA scores: opt: 147, E(): 3.5e-05, (92.85% identity in 28 aa overlap); etc.; PE family protein PE27A 3378415 PE27A 3205087 PE27A Mycobacterium tuberculosis H37Rv PE family protein PE27A YP_007411731.1 3378329 R 83332 CDS NP_217535.1 15610156 888926 complement(3378711..3379001) 1 NC_000962.3 Rv3019c, (MT3104, MTV012.33c), len: 96 aa. EsxR,secreted ESAT-6 like protein (see citations below), most similar to O53693|AAK44525|Rv0288|CFP7|MT0301|MTV035.16 10 KDA antigen CFP7 (low molecular weight protein antigen 7) (CFP-7) from Mycobacterium tuberculosis (95 aa), FASTA scores: opt: 566, E(): 5.1e-31, (84.3% identity in 95 aa overlap). Also similar to Q9CD33|ML2531 possible cell surface protein from Mycobacterium leprae (96 aa), FASTA scores: opt: 472, E(): 8.3e-25, (66.6% identity in 96 aa overlap); O53264|Rv3017c|MTV012.31c putative secreted antigen from Mycobacterium tuberculosis (120 aa), FASTA scores: opt: 321, E(): 9.6e-15, (67.15% identity in 70 aa overlap); Q57165|AAK48357|O84901|X79562|ESAT6|Rv3875|MT3989|MTV027.1 0esat6 gene from Mycobacterium tuberculosis strain Erdman (94 aa), FASTA scores: opt: 131, E(): 0.028, (26.1% identity in 88 aa overlap). Belongs to the ESAT6 family.; Secreted ESAT-6 like protein EsxR (TB10.3) (ESAT-6 like protein 9) 3379001 esxR 888926 esxR Mycobacterium tuberculosis H37Rv Secreted ESAT-6 like protein EsxR (TB10.3) (ESAT-6 like protein 9) NP_217535.1 3378711 R 83332 CDS YP_177919.1 57117047 888946 complement(3379036..3379329) 1 NC_000962.3 Rv3020c, (MTV012.34c), len: 97 aa. EsxS, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to AAK44524|MT0300 PE family protein from M. tuberculosis strain CDC1551 (97 aa), FASTA scores: opt: 564, E(): 5.9e-30, (91.75% identity in 97 aa overlap). Has potential helix-turn-helix motif at positions 14-35. Seems to belong to the ESAT6 family (see Betts et al., 2002). Note that previously known as PE28. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; ESAT-6 like protein EsxS 3379329 esxS 888946 esxS Mycobacterium tuberculosis H37Rv ESAT-6 like protein EsxS YP_177919.1 3379036 R 83332 CDS YP_177685.1 57117050 3205088 complement(3380679..3380993) 1 NC_000962.3 Rv3022A, len: 104 aa. PE29, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), similar to many others e.g. Rv0285|AL021930_12 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 497, E(): 3e-21, (80.39% identity in 102 aa overlap); etc.; PE family protein PE29 3380993 PE29 3205088 PE29 Mycobacterium tuberculosis H37Rv PE family protein PE29 YP_177685.1 3380679 R 83332 CDS NP_217539.1 15610160 888525 complement(3381375..3382622) 1 NC_000962.3 Rv3023c, (MTV012.38c), len: 415 aa. Probable IS1081 transposase. Contains PS01007 Transposases, Mutator family,signature. Similars to P35882|TRA1_MYCTU|Rv1199c|MTCI364.11c and Rv2512c|MTCY07A7.18c transposases for insertion sequence element IS1081 (415 aa), FASTA scores: opt: 2675, E(): 1.8e-162, (100.0% identity in 415 aa overlap). Belongs to the mutator family of transposase.; Probable transposase 3382622 888525 Rv3023c Mycobacterium tuberculosis H37Rv Probable transposase NP_217539.1 3381375 R 83332 CDS NP_217540.1 15610161 888524 complement(3382785..3383888) 1 NC_000962.3 Rv3024c, (MT3108, MTV012.39c), len: 367 aa. Probable trmU, tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase ,equivalent to O33099|TRMU_MYCLE|ML1707|MLCB637.07 probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2033, E(): 5.5e-116, (85.45% identity in 357 aa overlap). Also highly similar to others e.g. O86583|TRMU_STRCO|SC2A11.22 from Streptomyces coelicolor (376 aa), FASTA scores: opt: 1336, E(): 1e-73, (56.9% identity in 369 aa overlap); BAB49856|MLR2824 from Rhizobium loti (378 aa), FASTA scores: opt: 826, E(): 8.3e-43, (42.35% identity in 359 aa overlap); Q9ZDM1|TRMU_RICPR|RP306 from Rickettsia prowazekii (358 aa), FASTA scores: opt: 800, E(): 3e-41, (40.1% identity in 359 aa overlap); etc. Belongs to the TrmU family.; Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU 3383888 trmU 888524 trmU Mycobacterium tuberculosis H37Rv Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU NP_217540.1 3382785 R 83332 CDS NP_217541.1 15610162 887677 complement(3383885..3385066) 1 NC_000962.3 Rv3025c, (MTV012.40c), len: 393 aa. IscS (alternate gene name: nifS), cysteine desulfurase (NifS-like protein) , equivalent to MLCB637.06|O33098 NIFS-like protein from Mycobacterium leprae (396 aa), FASTA scores: opt: 2186,E(): 2.7e-122, (84.9% identity in 391 aa overlap). Also highly similar to many e.g. O86581|SC2A11.20 putative pyridoxal-phosphate-dependent aminotransferase from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1568,E(): 1.1e-85, (61.7% identity in 389 aa overlap); P57795|ISCS|NIFS cysteine desulfurase (NIFS protein homolog) from Methanosarcina thermophila (404 aa), FASTA scores: opt: 1059, E(): 1.6e-55, (46.2% identity in 381 aa overlap); O54055|ISCS_RUMFL|ISCS|NIFS cysteine desulfurase from Ruminococcus flavefaciens (396 aa), FASTA scores: opt: 973, E(): 2e-50, (43.3% identity in 381 aa overlap); P57794|NIFS_ACEDI cysteine desulfurase from Acetobacter diazotrophicus (400 aa), FASTA scores: opt: 958, E(): 1.6e-49, (41.1% identity in 392 aa overlap); etc. Also similar to Rv1464|MTV007.11 from Mycobacterium tuberculosis. Contains PS00595 Aminotransferases class-V pyridoxal-phosphate attachment site. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases, NIFS/ISCS subfamily. Cofactor: pyridoxal phosphate (by similarity).; Cysteine desulfurase IscS (NIFS protein homolog) (nitrogenase metalloclusters biosynthesis protein NIFS) 3385066 iscS 887677 iscS Mycobacterium tuberculosis H37Rv Cysteine desulfurase IscS (NIFS protein homolog) (nitrogenase metalloclusters biosynthesis protein NIFS) NP_217541.1 3383885 R 83332 CDS NP_217542.1 15610163 888489 complement(3385163..3386077) 1 NC_000962.3 Rv3026c, (MTV012.41c), len: 304 aa. Conserved hypothetical protein, similar to Q9RCZ0|SCM10.08C putative acyltransferase from Streptomyces coelicolor (275 aa),FASTA scores: opt: 393, E(): 2.2e-17, (41.4% identity in 299 aa overlap). Similar in part to other hypothetical proteins and acyltransferases e.g. BAB51968|MLR5533 from Rhizobium loti (266 aa), FASTA scores: opt: 280, E(): 2.4e-10, (29.45% identity in 258 aa overlap); Q9KIH9 putative acyltransferase (putative acyltransferase transmembrane protein) from Rhizobium meliloti (Sinorhizobium meliloti) (292 aa), FASTA scores: opt: 252,E(): 1.4e-08, (30.5% identity in 210 aa overlap); O69114|PLSC putative 1-acyl-SN-glycerol-3-phosphate acyltransferase from Burkholderia pseudomallei (Pseudomonas pseudomallei) (289 aa), FASTA scores: opt: 216, E(): 2.4e-06, (30.85% identity in 269 aa overlap); etc. So may be a member of acyltransferase family protein.; hypothetical protein 3386077 888489 Rv3026c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217542.1 3385163 R 83332 CDS NP_217543.2 448824798 887851 complement(3386074..3386919) 1 NC_000962.3 Rv3027c, (MTV012.42c), len: 281 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part. See Vetting et al. 2005. Similar, to others e.g. Q9RCY9|SCM10.09c from Streptomyces coelicolor (256 aa), FASTA scores: opt: 498,E(): 7.8e-24, (47.7% identity in 237 aa overlap); BAB50158|MLR3216 from Rhizobium loti (291 aa), FASTA scores: opt: 359, E(): 3.7e-15, (33.35% identity in 246 aa overlap); etc. Start changed since first submission,extended by 25 aa.; GCN5-related N-acetyltransferase 3386919 887851 Rv3027c Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase NP_217543.2 3386074 R 83332 CDS NP_217544.1 15610165 888549 complement(3387075..3388031) 1 NC_000962.3 Rv3028c, (MTV012.43c), len: 318 aa. Probable fixB (alternate gene name: etfA), electron transfer flavoprotein (alpha subunit) for various dehydrogenases. Equivalent to O33096|ETFA_MYCLE|FIXB|ML1711|MLCB637.04 electron transfer flavoprotein from Mycobacterium leprae (318 aa), FASTA scores: opt: 1788, E(): 1.1e-87, (89.3% identity in 318 aa overlap). Also highly similar to many e.g. Q9K418|SCG22.27c from Streptomyces coelicolor (320 aa), FASTA scores: opt: 1161, E(): 1.6e-54, (59.45% identity in 323 aa overlap); AAK08137|etfa from Rhodobacter sphaeroides (308 aa), FASTA scores: opt: 792, E(): 5.1e-35, (45.95% identity in 309 aa overlap); P38974|ETFA_PARDE electron transfer flavoprotein from Paracoccus denitrificans (307 aa), FASTA scores: opt: 789, E(): 7.4e-35, (45.95% identity in 309 aa overlap); etc. Belongs to the Etf alpha-subunit / FixB family.; Probable electron transfer flavoprotein (alpha-subunit) FixB (alpha-ETF) (electron transfer flavoprotein large subunit) (ETFLS) 3388031 fixB 888549 fixB Mycobacterium tuberculosis H37Rv Probable electron transfer flavoprotein (alpha-subunit) FixB (alpha-ETF) (electron transfer flavoprotein large subunit) (ETFLS) NP_217544.1 3387075 R 83332 CDS NP_217545.1 15610166 887670 complement(3388070..3388870) 1 NC_000962.3 Rv3029c, (MTV012.44c), len: 266 aa. Probable fixA (alternate gene name: etfB), electron transfer flavoprotein (beta-subunit). Equivalent of O33095|ETFB_MYCLE|FixA|MLCB637.03 electron transfer flavoprotein from Mycobacterium leprae (266 aa), FASTA scores: opt: 1603, E(): 7.6e-87, (95.1% identity in 266 aa overlap). Also highly similar to others e.g. Q9K417|SCG22.28c from Streptomyces coelicolor (262 aa),FASTA scores: opt: 860, E(): 2.3e-43, (52.4% identity in 263 aa overlap); O85691|ETFB_MEGEL from Megasphaera elsdenii (270 aa), FASTA scores: opt: 548, E(): 4.2e-25,(35.15% identity in 273 aa overlap); etc. Also highly similar in particular to Q9KHD0|NONH flavoprotein reductase from Streptomyces griseus subsp. griseus (this one is required for macrotetrolide biosynthesis in Streptomyces griseus) (261 aa), FASTA scores: opt: 867, E(): 8.8e-44,(54.0% identity in 263 aa overlap). Belongs to the Etf beta-subunit / FixA family.; Probable electron transfer flavoprotein (beta-subunit) FixA (beta-ETF) (electron transfer flavoprotein small subunit) (ETFSS) 3388870 fixA 887670 fixA Mycobacterium tuberculosis H37Rv Probable electron transfer flavoprotein (beta-subunit) FixA (beta-ETF) (electron transfer flavoprotein small subunit) (ETFSS) NP_217545.1 3388070 R 83332 CDS NP_217546.1 15610167 888604 3389101..3389925 1 NC_000962.3 Rv3030, (MTV012.45), len: 274 aa. Conserved protein,equivalent to O33094|MLCB637.02c|ML1713 hypothetical 30.8 KDa protein from Mycobacterium leprae (280 aa), FASTA scores: opt: 1388, E(): 5.5e-83, (78.2% identity in 280 aa overlap). N-terminus has similarity to hypothetical proteins from a number of organisms and to Q54303|EMBL:X86780|RAPM methyltransferase from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 191, E(): 3.6e-05, (35.65% identity in 101 aa overlap).; hypothetical protein 3389925 888604 Rv3030 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217546.1 3389101 D 83332 CDS NP_217547.1 15610168 888543 3389922..3391502 1 NC_000962.3 Rv3031, (MTV012.46), len: 526 aa. Conserved protein,equivalent to Q9CBR4|ML1714 hypothetical protein from Mycobacterium leprae (522 aa), FASTA scores: opt: 3167,E(): 4.4e-190, (86.15% identity in 526 aa overlap); and highly similar to truncated O33093|MLCB637.01c hypothetical 37.2 KDA protein (fragment) from Mycobacterium leprae (338 aa), FASTA scores: opt: 2041, E(): 5.7e-120, (84.8% identity in 342 aa overlap). Also some similarity to hypothetical proteins Q9V0M7|PAB1857 from Pyrococcus abyssi (602 aa), FASTA scores: opt: 477, E(): 3.5e-22, (31.2% identity in 556 aa overlap); and Synechocystis P74630|D90916|SLL0735 from Synechocystis sp. strain PCC 6803 (529 aa), FASTA scores: opt: 282, E(): 4.7e-10, (28.6% identity in 560 aa overlap).; hypothetical protein 3391502 888543 Rv3031 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217547.1 3389922 D 83332 CDS NP_217548.1 15610169 888185 3391534..3392778 1 NC_000962.3 Rv3032, (MTV012.47), len: 414 aa. Alpha (1->4) glucosyltransferase (See Stadthagen et al., 2007). Equivalent to Q9CBR3|ML1715 putative transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2456,E(): 7.3e-145, (87.9% identity in 414 aa overlap). Also similar to hypothetical proteins and various transferases e.g. P73369|SLL1971 hypothetical 46.2 KDA protein from Synechocystis sp. strain PCC 6803 (404 aa), FASTA scores: opt: 584, E(): 7.3e-29, (34.5% identity in 400 aa overlap); Q9Z5B7|SC2G5.06 putative transferase from Streptomyces coelicolor (406 aa), FASTA scores: opt: 509, E(): 3.3e-24,(35.9% identity in 413 aa overlap); Q9UZA1|PAB0827 galactosyltransferase (LPS biosynthesis RFBU related protein) from Pyrococcus abyssi (371 aa), FASTA scores: opt: 381, E(): 2.6e-16, (26.75% identity in 404 aa overlap); etc.; Alpha (1->4) glucosyltransferase 3392778 888185 Rv3032 Mycobacterium tuberculosis H37Rv Alpha (1->4) glucosyltransferase NP_217548.1 3391534 D 83332 CDS YP_004837058.1 345462031 11117812 3392812..3393201 1 NC_000962.3 Rv3032A, len: 129 aa. Conserved protein.; hypothetical protein 3393201 11117812 Rv3032A Mycobacterium tuberculosis H37Rv hypothetical protein YP_004837058.1 3392812 D 83332 CDS NP_217549.1 15610170 888865 3393380..3393928 1 NC_000962.3 Rv3033, (MTV012.48), len: 182 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 3393928 888865 Rv3033 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217549.1 3393380 D 83332 CDS NP_217550.1 15610171 887470 complement(3394019..3394921) 1 NC_000962.3 Rv3034c, (MTV012.49c), len: 300 aa. Possible transferase (2.-.-.-), equivalent to AAK47449|MT3119 Hexapeptide transferase family protein from M. tuberculosis strain CDC1551 but N-terminus shorter 39 residues (262 aa),FASTA scores: opt: 1773, E(): 4.7e-105, (100.0% identity in 262 aa overlap). Similar to Q9CBR1|ML1719 from Mycobacterium leprae but also shorter in N-terminus (245 aa), FASTA scores: opt: 1549, E(): 6.6e-91, (90.6% identity in 244 aa overlap). Some weakly similarity with other transferases (C-terminal part shows some similarity to acetyltransferase from Methanococcus jannaschii (214 aa)). Alternative start possible at 3395077 but codon usage not as good.; Possible transferase 3394921 887470 Rv3034c Mycobacterium tuberculosis H37Rv Possible transferase NP_217550.1 3394019 R 83332 CDS NP_217551.1 15610172 887842 3395379..3396461 1 NC_000962.3 Rv3035, (MTV012.50), len: 360 aa. Conserved protein,equivalent to Q9CBR0|ML1720 hypothetical protein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1963,E(): 1.4e-108, (75.8% identity in 363 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3396461 887842 Rv3035 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217551.1 3395379 D 83332 CDS NP_217552.1 15610173 887320 complement(3396458..3397141) 1 NC_000962.3 Rv3036c, (MTV012.51c), len: 227 aa. Probable TB22.2,conserved secreted protein, with putative N-terminal signal peptide, highly similar to secreted immunogenic protein MPT64/MPB64 P19996|Rv1980c|MTCY39.39 from Mycobacterium tuberculosis and Mycobacterium bovis (228 aa), FASTA scores: opt: 681, E(): 2.5e-35, (45.8% identity in 227 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved secreted protein TB22.2 3397141 TB22.2 887320 TB22.2 Mycobacterium tuberculosis H37Rv Probable conserved secreted protein TB22.2 NP_217552.1 3396458 R 83332 CDS NP_217553.1 15610174 888640 complement(3397214..3398290) 1 NC_000962.3 Rv3037c, (MTV012.52c), len: 358 aa. Conserved hypothetical protein, similar in part to others e.g. O86799|SC6G4.36c from Streptomyces coelicolor (426 aa),FASTA scores: opt: 545, E(): 5.5e-27, (36.15% identity in 354 aa overlap); Q9UZW6|PAB0687 from Pyrococcus abyssi (386 aa), FASTA scores: opt: 262, E(): 3.5e-09, (31.0% identity in 200 aa overlap).; hypothetical protein 3398290 888640 Rv3037c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217553.1 3397214 R 83332 CDS NP_217554.1 15610175 888517 complement(3398425..3399408) 1 NC_000962.3 Rv3038c, (MTV012.53c), len: 327 aa. Conserved protein, equivalent to Q9CBQ9|ML1723 hypothetical protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1843, E(): 6.1e-108, (80.75% identity in 327 aa overlap). Weak similarity with e.g. Q9KZI3|SCG8A.16 putative methyltransferase from Streptomyces coelicolor (199 aa),FASTA scores: opt: 227, E(): 3.9e-07, (31.95% identity in 191 aa overlap) and O52570 methyltransferase from Amycolatopsis mediterranei (272 aa), FASTA scores: opt: 228, E(): 4.3e-07, (31.7% identity in 164 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature but shows no similarity to known LysR family members.; hypothetical protein 3399408 888517 Rv3038c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217554.1 3398425 R 83332 CDS NP_217555.1 15610176 888216 complement(3399419..3400183) 1 NC_000962.3 Rv3039c, (MTV012.54c), len: 254 aa. Probable echA17,Enoyl-CoA Hydratase/Isomerase Superfamily member (crotonase). Similar to many e.g. Q9L1E6|SC3D11.16 putative enoyl-CoA hydratase from Streptomyces coelicolor (255 aa),FASTA scores: opt: 625, E(): 1.5e-30, (45.55% identity in 224 aa overlap); O07137||ECH8_MYCLE|ML2402|MLCB1306.05c probable enoyl-CoA hydratase ECHA8 from Mycobacterium leprae (257 aa), FASTA scores: opt: 448, E(): 6.4e-20,(35.3% identity in 235 aa overlap), P97087|CRT crotonase / enoyl-CoA hydratase from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa),FASTA scores: opt: 420, E(): 3.1e-18, (31.2% identity in 234 aa overlap). Also similar to Mycobacterium tuberculosis AAK45356|O53418|Rv1070c|ECHA8|MT1100|MTV017.23c probable enoyl-CoA hydratase ECHA8 (257 aa), FASTA scores: opt: 450,E(): 4.9e-20, (36.4% identity in 226 aa overlap). Belongs to the enoyl-CoA hydratase/isomerase family.; Probable enoyl-CoA hydratase EchA17 (crotonase) (unsatured acyl-CoA hydratase) (enoyl hydrase) 3400183 echA17 888216 echA17 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA17 (crotonase) (unsatured acyl-CoA hydratase) (enoyl hydrase) NP_217555.1 3399419 R 83332 CDS NP_217556.1 15610177 887672 complement(3400192..3401058) 1 NC_000962.3 Rv3040c, (MTV012.55c), len: 288 aa. Conserved protein, highly similar to Q9XA40|SCH17.07c hypothetical protein from Streptomyces coelicolor (312 aa), FASTA scores: opt: 648, E(): 5.2e-34, (50.0% identity in 260 aa overlap). Also similar to Q9F7R7 predicted mutt superfamily hydrolase from uncultured proteobacterium EBAC31A08 (264 aa), FASTA scores: opt: 295, E(): 1.3e-11, (27.2% identity in 257 aa overlap); AAK24293|CC2322 hypothetical protein from Caulobacter crescentus (254 aa), blast scores: 185 (32% identity) and 131 (37% identity), etc.; hypothetical protein 3401058 887672 Rv3040c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217556.1 3400192 R 83332 CDS NP_217557.1 15610178 887197 complement(3401055..3401918) 1 NC_000962.3 Rv3041c, (MTV012.56c), len: 287 aa. Probable conserved ATP-binding protein ABC transporter (see citation below), equivalent to Q9CBQ7|ML1726 putative ABC transporter protein ATP-binding protein from Mycobacterium leprae (305 aa), FASTA scores: opt: 1576, E(): 8.6e-85,(83.4% identity in 289 aa overlap). Also similar to other putative ATP-binding proteins ABC transporters e.g. Q9X9Z4|SCI5.06C from Streptomyces coelicolor (265 aa),FASTA scores: opt: 893, E(): 4.8e-45, (53.3% identity in 257 aa overlap); Q9L156|SC5C11.16c from Streptomyces coelicolor (279 aa), FASTA scores: opt: 680, E(): 1.3e-32,(45.4% identity in 271 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable conserved ATP-binding protein ABC transporter 3401918 887197 Rv3041c Mycobacterium tuberculosis H37Rv Probable conserved ATP-binding protein ABC transporter NP_217557.1 3401055 R 83332 CDS NP_217558.1 15610179 887815 complement(3401933..3403162) 1 NC_000962.3 Rv3042c, (MTV012.57c), len: 409 aa. Probable serB2,Phosphoserine phosphatase, equivalent to Q9CBQ6|ML1727 putative phosphoserine phosphatase from Mycobacterium leprae (411 aa), FASTA scores: opt: 2173, E(): 1.3e-117,(86.3% identity in 408 aa overlap). Also similar to other e.g. Q9S281|SCI28.02 from Streptomyces coelicolor (410 aa),FASTA scores: opt: 1209, E(): 3e-62, (51.75% identity in 400 aa overlap); Q9HUK|PA4960 from Pseudomonas aeruginosa (429 aa), FASTA scores: opt: 704, E(): 3.1e-33, (40.95% identity in 393 aa overlap); O28142|SERB_ARCTU|AF2138 from Archaeoglobus fulgidus (344 aa), FASTA scores: opt: 671,E(): 2e-31, (37.25% identity in 325 aa overlap); and P06862|SERB_ECOLI (322 aa), FASTA scores: opt: 628, E(): 5.7e-29, (46.8% identity in 235 aa overlap). Belongs to the SerB family.; Probable phosphoserine phosphatase SerB2 (PSP) (O-phosphoserine phosphohydrolase) (pspase) 3403162 serB2 887815 serB2 Mycobacterium tuberculosis H37Rv Probable phosphoserine phosphatase SerB2 (PSP) (O-phosphoserine phosphohydrolase) (pspase) NP_217558.1 3401933 R 83332 CDS NP_217559.1 15610180 887881 complement(3403200..3404921) 1 NC_000962.3 Rv3043c, (MTV012.58c), len: 573 aa. Probable ctaD,integral membrane cytochrome C oxidase polypeptide I,equivalent to Q9CBQ5|ML1728 from Mycobacterium leprae (574 aa), FASTA scores: opt: 3738, E(): 3.8e-216, (95.4% identity in 566 aa overlap). Also similar to other cytochrome C oxidases polypeptide I e.g. Q9AEL9|CTAD from Corynebacterium glutamicum (Brevibacterium flavum) (584 aa), FASTA scores: opt: 3065, E(): 6.8e-176, (72.65% identity in 567 aa overlap); Q9X813|SC6G10.28c from Streptomyces coelicolor (578 aa), FASTA scores: opt: 2888,E(): 2.6e-165, (71.7% identity in 544 aa overlap); Q9K451|CTAD from Streptomyces coelicolor (573 aa), FASTA scores: opt: 2757, E(): 1.8e-157, (70.2% identity in 537 aa overlap). Contains PS00077 Cytochrome c oxidase subunit I,copper B binding region signature. Belongs to the heme-copper respiratory oxidase family.; Probable cytochrome C oxidase polypeptide I CtaD (cytochrome AA3 subunit 1) 3404921 ctaD 887881 ctaD Mycobacterium tuberculosis H37Rv Probable cytochrome C oxidase polypeptide I CtaD (cytochrome AA3 subunit 1) NP_217559.1 3403200 R 83332 CDS NP_217560.1 15610181 888745 3405136..3406215 1 NC_000962.3 Rv3044, (MTV012.59), len: 359 aa. Probable fecB,FeIII dicitrate-binding periplasmic lipoprotein (see citation below), equivalent to Q9CBQ4|FECB|ML1729 putative FEIII-dicitrate transporter lipoprotein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1816, E(): 1.1e-96,(75.65% identity in 357 aa overlap); and Q9LA57|FECB from Mycobacterium avium (364 aa), FASTA scores: opt: 1769, E(): 5.1e-94. Similar to many periplasmic FeIII-dicitrate transporters e.g. P72593|FECB|SLR1319 from Synechocystis sp. strain PCC 6803 (315 aa), FASTA scores: opt: 459, E(): 3.6e-19, (31.35% identity in 303 aa overlap); and P72611|FECB|SLR1492 from Synechocystis sp. strain PCC 6803. N-terminus longer (approximately 30 aa) to AAK47459 from Mycobacterium tuberculosis strain CDC1551 (327 aa). Has signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable FEIII-dicitrate-binding periplasmic lipoprotein FecB 3406215 fecB 888745 fecB Mycobacterium tuberculosis H37Rv Probable FEIII-dicitrate-binding periplasmic lipoprotein FecB NP_217560.1 3405136 D 83332 CDS NP_217561.1 15610182 888888 3406285..3407325 1 NC_000962.3 Rv3045, (MTV012.60), len: 346 aa. Probable adhC,NADP-dependent alcohol dehydrogenase, equivalent to Q9CBQ3|ADHA|ML1730 alcohol dehydrogenases from Mycobacterium leprae (362 aa), FASTA scores: opt: 1982,E(): 1.3e-111, (85.85% identity in 346 aa overlap); Q9AE96|ADHC from Mycobacterium smegmatis (348 aa), FASTA scores: opt: 1808, E(): 3.4e-101, (78.95% identity in 347 aa overlap); Q9EWF1|SCK13.33c putative dehydrogenase from Streptomyces coelicolor (346 aa), FASTA scores: opt: 1508,E(): 3.3e-83, (64.45% identity in 346 aa overlap); O06007|ADHA from Bacillus subtilis (349 aa), FASTA scores: opt: 1412, E(): 1.9e-77, (61.8% identity in 335 aa overlap); etc. Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family. High similarity with other bacterial ADH'S.; Probable NADP-dependent alcohol dehydrogenase AdhC 3407325 adhC 888888 adhC Mycobacterium tuberculosis H37Rv Probable NADP-dependent alcohol dehydrogenase AdhC NP_217561.1 3406285 D 83332 CDS NP_217562.1 15610183 888870 complement(3407314..3407688) 1 NC_000962.3 Rv3046c, (MTV012.61c), len: 124 aa. Conserved protein, similar to several hypothetical mycobacterial proteins e.g. Q50171|ML2258 U296W hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 194, E(): 7.6e-06, (35.9% identity in 103 aa overlap); and O06409|Rv0543c|MTCY25D10.22c from Mycobacterium tuberculosis (100 aa), FASTA scores: opt: 192, E(): 1e-05,(34.7% identity in 98 aa overlap).; hypothetical protein 3407688 888870 Rv3046c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217562.1 3407314 R 83332 CDS NP_217563.1 15610184 888898 complement(3408022..3408306) 1 NC_000962.3 Rv3047c, (MTV012.62c), len: 94 aa. Hypothetical unknown protein.; Hypothetical protein 3408306 888898 Rv3047c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217563.1 3408022 R 83332 CDS YP_177921.1 57117051 888886 complement(3408404..3409378) 1 NC_000962.3 Rv3048c, (MTV012.63c), len: 324 aa. NrdF2,ribonucleoside-diphosphate reductase, beta chain (see citation below), equivalent to Q9CBQ2|RIR2_MYCL|NRDF|ML1731 ribonucleoside-diphosphate reductase beta chain from Mycobacterium leprae (325 aa), FASTA scores: opt: 2009,E(): 1.3e-123, (93.5% identity in 324 aa overlap). Also similar to other ribonucleoside-diphosphate reductases e.g. Q9XD62|NRDF from Corynebacterium glutamicum (Brevibacterium flavum) (334 aa), FASTA scores: opt: 1648, E(): 4.2e-100,(78.35% identity in 314 aa overlap); O69274|NRDF from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (329 aa), FASTA scores: opt: 1626, E(): 1.1e-98, (75.3% identity in 320 aa overlap); P37146|NRDF|B2676 from Escherichia coli (319 aa), FASTA scores: opt: 1569, E(): 5.7e-95, (71.3% identity in 317 aa overlap). Contains PS00368 Ribonucleotide reductase small subunit signature. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: binds 2 iron ions (by similarity). Note that previously known as nrdG.; Ribonucleoside-diphosphate reductase (beta chain) NrdF2 (ribonucleotide reductase small subunit) (R2F protein) 3409378 nrdF2 888886 nrdF2 Mycobacterium tuberculosis H37Rv Ribonucleoside-diphosphate reductase (beta chain) NrdF2 (ribonucleotide reductase small subunit) (R2F protein) YP_177921.1 3408404 R 83332 CDS NP_217565.1 15610186 888894 complement(3409509..3411083) 1 NC_000962.3 Rv3049c, (MTV012.64c), len: 524 aa. Probable monooxygenase, similar to several monooxygenases e.g. Q9I3H5|PA1538 probable flavin-containing monooxygenase from Pseudomonas aeruginosa (527 aa), FASTA scores: opt: 1577,E(): 3.9e-90, (47.3% identity in 501 aa overlap); Q9RKB5|SCE87.23c monooxygenase from Streptomyces coelicolor (519 aa), FASTA scores: opt: 1522, E(): 9.8e-87, (47.4% identity in 485 aa overlap); Q9I218|PA2097 probable flavin-binding monooxygenase from Pseudomonas aeruginosa (491 aa), FASTA scores: opt: 1366, E(): 4.2e-77, (43.75% identity in 489 aa overlap); etc. Also similar to Q10532|Rv0892|Y892_MYCTU|MT0916|MTCY31.20 probable monooxygenase from Mycobacterium tuberculosis strain H37Rv (495 aa), FASTA scores: opt: 1147, E(): 1.5e-63, (38.0% identity in 479 aa overlap).; Probable monooxygenase 3411083 888894 Rv3049c Mycobacterium tuberculosis H37Rv Probable monooxygenase NP_217565.1 3409509 R 83332 CDS NP_217566.1 15610187 888866 complement(3411217..3411957) 1 NC_000962.3 Rv3050c, (MTV012.65c), len: 246 aa. Probable transcriptional regulatory protein TetR-family, equivalent but shorter to Q9CBQ1|ML1733 from Mycobacterium leprae (275 aa), FASTA scores: opt: 1381,(E): 2.7e-79, (86.25% identity in 240 aa overlap); AAK44712|MT0489 from Mycobacterium tuberculosis strain CDC1551 (256 aa), FASTA scores: opt: 328,(E): 1.8e-13, (30.75% identity in 234 aa overlap); etc. Also some similarity to O53757|Rv0472c|MTV038.16c. Alternative starts possible at 68052 or 67923. Has potential helix-turn-helix motif at positons 51-72.; Probable transcriptional regulatory protein (probably AsnC-family) 3411957 888866 Rv3050c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably AsnC-family) NP_217566.1 3411217 R 83332 CDS NP_217567.1 15610188 888869 complement(3412085..3414166) 1 NC_000962.3 Rv3051c, (MTV012.66c), len: 693 aa. NrdE,ribonucleotide-diphosphate reductase, alpha chain (see citations below), equivalent to Q9CBQ0|NRDE|ML1734 from Mycobacterium leprae (693 aa), FASTA scores: opt: 4259,E(): 0, (93.2% identity in 693 aa overlap). Similar to other Ribonucleoside-diphosphate reductases e.g. Q9XD63|NRDE from Corynebacterium glutamicum (Brevibacterium flavum) (707 aa), FASTA scores: opt: 3683,E(): 0, (79.35% identity in 693 aa overlap); O69273|NRDE from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (720 aa), FASTA scores: opt: 3555, E(): 1.7e-214, (76.1% identity in 694 aa overlap); P39452|NRDE|B2675 from Escherichia coli (713 aa),FASTA scores: opt: 3430, E(): 1.1e-206, (73.6% identity in 693 aa overlap); etc. Equivalent to AAK47468|MT3137 from Mycobacterium tuberculosis strain CDC1551 (725 aa) but shorter in N-terminus. Contains PS00089 Ribonucleotide reductase large subunit signature. Belongs to the ribonucleoside diphosphate reductase large chain family.; Ribonucleoside-diphosphate reductase (alpha chain) NrdE (ribonucleotide reductase small subunit) (R1F protein) 3414166 nrdE 888869 nrdE Mycobacterium tuberculosis H37Rv Ribonucleoside-diphosphate reductase (alpha chain) NrdE (ribonucleotide reductase small subunit) (R1F protein) NP_217567.1 3412085 R 83332 CDS NP_217568.1 15610189 888885 complement(3414232..3414684) 1 NC_000962.3 Rv3052c, (MTCY22D7.30), len: 150 aa. Probable nrdI,equivalent to Q9CBP9|NRDI|ML1735 from Mycobacterium leprae (138 aa), FASTA scores: opt: 765, E(): 3.8e-44, (79.7% identity in 138 aa overlap), and similar to many NRDI proteins e.g. Q47415|NRDI_ECOLI|B2674 from Escherichia coli (136 aa), FASTA scores: opt: 574, E(): 1.9e-31, (62.2% identity in 135 aa overlap). Belongs to the NRDI family.; Probable NrdI protein 3414684 nrdI 888885 nrdI Mycobacterium tuberculosis H37Rv Probable NrdI protein NP_217568.1 3414232 R 83332 CDS NP_217569.1 15610190 888884 complement(3414719..3414958) 1 NC_000962.3 Rv3053c, (MTCY22D7.29), len: 79 aa. Probable nrdH,glutaredoxin-like protein, equivalent to Q9CBP8|NRDH|ML1736 from Mycobacterium leprae (80 aa), FASTA scores: opt: 478,E(): 2.7e-27, (91.15% identity in 79 aa overlap), and similar to many glutaredoxin-like proteins e.g. Q9XD65|NRDH from Corynebacterium glutamicum (Brevibacterium flavum) (77 aa), FASTA scores: opt: 382, E(): 1.5e-20, (72.35% identity in 76 aa overlap); and Q56108|NRDH_SALTY from Salmonella typhimurium (81 aa), FASTA scores: opt: 243, E(): 9.9e-11,(45.85% identity in 72 aa overlap). Belongs to the glutaredoxin family.; Probable glutaredoxin electron transport component of NRDEF (glutaredoxin-like protein) NrdH 3414958 nrdH 888884 nrdH Mycobacterium tuberculosis H37Rv Probable glutaredoxin electron transport component of NRDEF (glutaredoxin-like protein) NrdH NP_217569.1 3414719 R 83332 CDS NP_217570.1 15610191 887963 complement(3415435..3415989) 1 NC_000962.3 Rv3054c, (MTCY22D7.28), len: 184 aa. Conserved hypothetical protein, similar to Q9RD22|SCM1.21 putative secreted protein from Streptomyces coelicolor (187 aa),FASTA scores: opt: 651, E(): 1.5e-33, (56.8% identity in 175 aa overlap). Also shares similarity with other hypothetical proteins and Chromate reductases e.g. AAK56853|CHRR from Pseudomonas putida (186 aa), FASTA scores: opt: 339, E(): 3.3e-14, (38.75% identity in 160 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599) near C-terminus.; hypothetical protein 3415989 887963 Rv3054c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217570.1 3415435 R 83332 CDS NP_217571.1 15610192 888882 3416081..3416695 1 NC_000962.3 Rv3055, (MTCY22D7.26c), len: 204 aa. Possible transcriptional regulatory protein, similar to Q9RD23|SCM1.20c putative TetR-family transcriptional regulator from Streptomyces coelicolor (234 aa), FASTA scores: opt: 471, E(): 4.6e-23, (44.9% identity in 187 aa overlap); and with low similarity to other e.g. Q9ADK8|2SCK31.12 putative TetR-family transcriptional regulator from Streptomyces coelicolor (198 aa), FASTA scores: opt: 208, 2.5e-06, (32.9% identity in 155 aa overlap); Q9ADD9|SCBAC20F6.11c putative TetR-family transcriptional from Streptomyces coelicolor (199 aa),FASTA scores: opt: 182, E(): 0.00012, (31.0% identity in 184 aa overlap). Contains potential helix-turn-helix motif from aa 48 to 69 (+3.42 SD). so may belong to the TetR/AcrR family of transcriptional regulators.; Possible transcriptional regulatory protein (probably TetR-family) 3416695 888882 Rv3055 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably TetR-family) NP_217571.1 3416081 D 83332 CDS NP_217572.1 15610193 887519 3416705..3417745 1 NC_000962.3 Rv3056, (MTCY22D7.25c, MT3142), len: 346 aa. Possible dinP (alternate gene name: dinB2),DNA-damage-inducible protein (DNA polymerase V) (see citations below), similar to others e.g. AAK45855|MT1589 from Mycobacterium tuberculosis strain CDC1551 (485 aa),FASTA scores: opt: 620, E(): 6.1e-32, (37.2% identity in 344 aa overlap); BAB49140|MLR1877 from Rhizobium loti (Mesorhizobium loti) (415 aa), FASTA scores: opt: 533, E(): 1.8e-26, (34.35% identity in 358 aa overlap); and BAB54888|MLL9709 from Rhizobium loti (Mesorhizobium loti) (361 aa), FASTA scores: opt: 532, E(): 1.8e-26, (35.35% identity in 348 aa overlap). Extensive similarity to proteins induced by DNA damage such as dinP, mucB, umuC. Belongs to the DNA polymerase type-Y family.; Possible DNA-damage-inducible protein P DinP (DNA polymerase V) (pol IV 2) (DNA nucleotidyltransferase (DNA-directed)) 3417745 dinP 887519 dinP Mycobacterium tuberculosis H37Rv Possible DNA-damage-inducible protein P DinP (DNA polymerase V) (pol IV 2) (DNA nucleotidyltransferase (DNA-directed)) NP_217572.1 3416705 D 83332 CDS NP_217573.1 15610194 888617 complement(3417799..3418662) 1 NC_000962.3 Rv3057c, (MTCY22D7.24), len: 287 aa. Probable oxidoreductase, probably short-chain alcohol dehydrogenase/reductase. Equivalent to Q9CBP7|ML1740 possible short chain dehydrogenases/reductase from Mycobacterium leprae (312 aa), FASTA scores: opt: 1563,E(): 6e-89, (81.8% identity in 280 aa overlap). Also similar to many oxidoreductases e.g. Q9ZBX8|SCD78.21c putative oxidoreductase from Streptomyces coelicolor (585 aa), FASTA scores: opt: 541, E(): 6.7e-26, (37.25% identity in 263 aa overlap); AAK47506|MT3170 oxidoreductase,short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 521, E(): 6.1e-25, (36.25% identity in 276 aa overlap); AAK45541|MT1283 oxidoreductase, short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 471, E(): 7.2e-22, (32.4% identity in 281 aa overlap). Also similar to O50460|Rv1245c|MTV006.17C dehydrogenase (276 aa). Contains short-chain alcohol dehydrogenase family signature (PS00061). May belong to the short-chain dehydrogenases/reductases (SDR) family.; Probable short chain alcohol dehydrogenase/reductase 3418662 888617 Rv3057c Mycobacterium tuberculosis H37Rv Probable short chain alcohol dehydrogenase/reductase NP_217573.1 3417799 R 83332 CDS NP_217574.1 15610195 888878 complement(3418726..3419376) 1 NC_000962.3 Rv3058c, (MTCY22D7.23), len: 216 aa. Possible transcriptional regulatory protein, TetR-family, showing reasonable similarity to others e.g. AAK48337|MT3970 from Mycobacterium tuberculosis strain CDC1551 (216 aa), FASTA scores: opt: 261, E(): 2.8e-10, (31.7% identity in 221 aa overlap); Q49962|ML1070|U1756B from Mycobacterium leprae (217 aa), FASTA scores: opt: 234, E(): 1.8e-08, (27.2% identity in 195 aa overlap); Q9CDD3|ML0064 from Mycobacterium leprae (214 aa), FASTA scores: opt: 199, E(): 3.6e-06, (25.65% identity in 195 aa overlap); O66121|CPRS from Streptomyces coelicolor (215 aa), FASTA scores: opt: 183, E(): 4.2e-05, (26.0% identity in 196 aa overlap). Equivalent to AAK47476|MT3144 from Mycobacterium tuberculosis strain CDC1551 (237 aa) but N-terminus shorter 21 residues. Start was predicted by TBParse but alternatives (ATG) are possible. Could belong to the TetR/AcrR family of transcriptional regulators.; Possible transcriptional regulatory protein (probably TetR-family) 3419376 888878 Rv3058c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably TetR-family) NP_217574.1 3418726 R 83332 CDS NP_217575.1 15610196 888883 3419492..3420970 1 NC_000962.3 Rv3059, (MTCY22D7.22c), len: 492 aa. Probable cyp136, cytochrome P450 136, similar to other cytochrome P450-dependent oxidases e.g. Q59990|CYP120|CYP|SLR0574 putative cytochrome P450 120 from Synechocystis sp. strain PCC 6803 (444 aa), FASTA scores: opt: 579, E(): 1.5e-29,(27.3% identity in 443 aa overlap); Q64654|CYP51|CP51_RAT cytochrome P450 51 (lanosterol 14-alpha demethylase) from Rattus norvegicus (Rat) (503 aa), FASTA scores: opt: 549,E(): 1.4e-27, (26.2% identity in 458 aa overlap); Q9JIY3|CYP51 lanosterol 14-alpha-demethylase from Mus musculus (Mouse) (486 aa), FASTA scores: opt: 546, E(): 2.1e-27, (25.75% identity in 458 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family.; Probable cytochrome P450 136 Cyp136 3420970 cyp136 888883 cyp136 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 136 Cyp136 NP_217575.1 3419492 D 83332 CDS NP_217576.1 15610197 888855 complement(3421741..3423213) 1 NC_000962.3 Rv3060c, (MTCY22D7.21), len: 490 aa. Probable transcriptional regulatory protein, showing reasonable similarity to several members of the GntR family e.g. BAB54431|MLL8575 from Rhizobium loti (Mesorhizobium loti) (247 aa), FASTA scores: opt: 274, E(): 3.5e-10, (30.35% identity in 224 aa overlap); P96570|ESMR from Burkholderia cepacia (Pseudomonas cepacia) (277 aa), FASTA scores: opt: 229, E(): 2.8e-07, (25.85% identity in 240 aa overlap); Q9S276|SCI28.07 from Streptomyces coelicolor (230 aa),FASTA scores: opt: 211, E(): 3.4e-06, (27.25% identity in 220 aa overlap); etc. Seems to have two domains: residues 1-260 resemble UxuR, and 260-490 resemble PdhR, ExuR, etc. Contains bacterial regulatory proteins, GntR family signature (PS00043). Helix-turn-helix motif (+3.13 SD) at aa 38-59. Seems to belong to the GntR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably GntR-family) 3423213 888855 Rv3060c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably GntR-family) NP_217576.1 3421741 R 83332 CDS NP_217577.1 15610198 887617 complement(3423262..3425427) 1 NC_000962.3 Rv3061c, (MTCY22D7.20), len: 721 aa. Probable fadE22, Acyl-CoA Dehydrogenase, similar to many e.g. AAK44503|MT0284 from Mycobacterium tuberculosis strain CDC1551 (731 aa), FASTA scores: opt: 1804, E(): 1.1e-101,(43.45% identity in 743 aa overlap); AAK48037|MT3678 from Mycobacterium tuberculosis strain CDC1551 (711 aa), FASTA scores: opt: 1630, E(): 3.9e-91, (42.55% identity in 733 aa overlap); and extensive similarity in C-terminal part to many acyl-CoA dehydrogenases e.g. Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 767,E(): 4.8e-39, (36.7% identity in 376 aa overlap). Also similar to many hypothetical proteins. Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE22 3425427 fadE22 887617 fadE22 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE22 NP_217577.1 3423262 R 83332 CDS NP_217578.1 15610199 887553 3425584..3427107 1 NC_000962.3 Rv3062, (MTCY22D7.19c), len: 507 aa. Probable ligB,DNA ligase ATP-dependent (see citation below), highly similar to numerous archaebacterial and eukaryotic polynucleotide DNA ligases, e.g. Q9FCB1|DNLI_STRCO|LIG|2SCG58.02 from Streptomyces coelicolor (512 aa), FASTA scores: opt: 1677, E(): 2.5e-90,(55.65% identity in 512 aa overlap); Q9HR35|DNLI_HALN1|LIG|VNG0881G from Halobacterium sp. strain NRC-1 (561 aa), FASTA scores: opt: 985, E(): 5.6e-50, (42.25% identity in 440 aa overlap); Q9V185|DNLI_PYRAB|LIG|PAB2002 from Pyrococcus abyssi (559 aa), FASTA scores: opt: 978, E(): 1.4e-49, (39.05% identity in 443 aa overlap); etc. Also similar to Rv3731|MTV025.079|LIGC possible DNA ligase from M. tuberculosis (358 aa). Similarity at N-terminus is poor so first start codon was taken. Contains (PS00697) ATP-dependent DNA ligase AMP-binding site signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-dependent DNA ligase family.; Probable ATP-dependent DNA ligase LigB (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase) 3427107 ligB 887553 ligB Mycobacterium tuberculosis H37Rv Probable ATP-dependent DNA ligase LigB (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase) NP_217578.1 3425584 D 83332 CDS NP_217579.1 15610200 887948 3427243..3429519 1 NC_000962.3 Rv3063, (MTCY22D7.18c), len: 758 aa. Probable cstA,integral membrane starvation-induced stress response protein, similar to other e.g. P15078|CSTA_ECOLI|B0598 from Escherichia coli strain K12 (701 aa), FASTA scores: opt: 2357, E(): 9.5e-137, (51.25% identity in 712 aa overlap); AAG54933|CSTA from Escherichia coli strain O157:H7 EDL933 (701 aa), FASTA scores: opt: 2356, E(): 1.1e-136, (51.1% identity in 712 aa overlap); etc. Predicted to be membrane associated. Similarity suggests start at GTG at 16801 in Y22D7 but no RBS obvious so TBParse-predicted start at 16881 taken. Belongs to the CstA family.; Probable carbon starvation protein A homolog CstA 3429519 cstA 887948 cstA Mycobacterium tuberculosis H37Rv Probable carbon starvation protein A homolog CstA NP_217579.1 3427243 D 83332 CDS NP_217580.1 15610201 888879 complement(3429825..3430250) 1 NC_000962.3 Rv3064c, (MTCY22D7.17), len: 141 aa. Probable conserved integral membrane protein, similar to many e.g. Q9KY40|SCC8A.08 putative integral membrane protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 391,E(): 2.4e-18, (48.45% identity in 130 aa overlap); Q9K461|SC2H12.23c putative integral membrane protein from Streptomyces coelicolor (151 aa), FASTA scores: opt: 339,E(): 5.1e-15, (46.7% identity in 124 aa overlap); BAB48975|MLR1652 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (130 aa), FASTA scores: opt: 319, E(): 8.7e-14, (41.45% identity in 123 aa overlap); Q9JR31|NMA2196|NMB0291 conserved hypothetical inner membrane protein from Neisseria meningitidis serogroup a and B (132 aa), FASTA scores: opt: 303, E(): 9.4e-13,(43.65% identity in 126 aa overlap); etc.; Probable conserved integral membrane protein 3430250 888879 Rv3064c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217580.1 3429825 R 83332 CDS YP_177922.1 57117052 887550 3430387..3430710 1 NC_000962.3 Rv3065, (MT3150.1, MTCY22D7.17c), len: 107 aa. Mmr,integral membrane multidrugs resistance transporter (see citation below), equivalent to Q9CBP1|ML1756 probable multidrug resistance protein from Mycobacterium leprae (107 aa), FASTA scores: opt: 534, E(): 3.3e-28, (77.55% identity in 107 aa overlap). Also highly similar to bacterial proteins involved in resistance to ethidium bromide or methyl viologen e.g. O87866|QACG_STASP quaternary ammonium compound-resistance protein QACG (quarternary ammonium determinant G) from Staphylococcus sp. strain ST94 (107 aa), FASTA scores: opt: 307, E(): 1.8e-13, (39.8% identity in 103 aa overlap); P96460|QAC quaternary ammonium compounds resistance protein QAC from Staphylococcus aureus (107 aa), FASTA scores: opt: 304, E(): 2.8e-13, (40.4% identity in 104 aa overlap); Q57225|QACE_ECOLI quaternary ammonium compound-resistance protein QACE (quarternary ammonium determinant E) from Escherichia coli (110 aa),FASTA scores: opt: 300, E(): 5.2e-13, (48.15% identity in 108 aa overlap); AAG55967|Z1870 methylviologen resistance protein encoded within prophage CP-933X from Escherichia coli strain O157:H7 EDL933 (110 aa); P23895|EMRE|MVRC|EB|B0543 EMRE protein from Escherichia coli (110 aa), FASTA scores: opt: 290, E(): 2.3e-12,(43.55% identity in 101 aa overlap); etc. Also similar to the SugE protein of enteric bacteria. Belongs to the small multidrug resistance (SMR) protein family. Note that previously known as emrE.; Multidrugs-transport integral membrane protein Mmr 3430710 mmr 887550 mmr Mycobacterium tuberculosis H37Rv Multidrugs-transport integral membrane protein Mmr YP_177922.1 3430387 D 83332 CDS NP_217582.1 15610203 888847 3430707..3431315 1 NC_000962.3 Rv3066, (MTCY22D7.15c), len: 202 aa. Probable transcriptional regulatory protein deoR-family, with some similarity to transcriptional regulators and hypothetical proteins, e.g. Q9X9V5|SCI7.35c hypothetical 21.1 KDA protein from Streptomyces coelicolor (197 aa), FASTA scores: opt: 398, E(): 5.7e-19, (40.3% identity in 191 aa overlap); AAG55222|Z1073 putative DeoR-type transcriptional regulator from Escherichia coli strain O157:H7 EDL933 (178 aa), FASTA scores: opt: 257, E(): 7.9e-10, (28.4% identity in 176 aa overlap); Q9HXU1|PA3699 probable transcriptional regulator (TetR/AcrR family) from Pseudomonas aeruginosa (237 aa), FASTA scores: opt: 229, E(): 6.7e-08, (32.1% identity in 187 aa overlap); etc.; Probable transcriptional regulatory protein (probably DeoR-family) 3431315 888847 Rv3066 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably DeoR-family) NP_217582.1 3430707 D 83332 CDS NP_217583.1 15610204 888822 3431428..3431838 1 NC_000962.3 Rv3067, (MTCY22D7.14c), len: 136 aa. Conserved hypothetical protein, weakly similar to other mycobacterium proteins e.g. O53953|Rv1804c|MTV049.26c (108 aa), FASTA scores: opt: 183, E(): 0.00053, (36.6% identity in 82 aa overlap); O07222|Rv1810|MTCY16F9.04c (118 aa), FASTA scores: opt: 149, E(): 0.05, (30.95% identity in 84 aa overlap). Has hydrophobic stretch at N-terminus. Start chosen on basis of codon usage but upstream ATG also possible.; hypothetical protein 3431838 888822 Rv3067 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217583.1 3431428 D 83332 CDS NP_217584.1 15610205 888826 complement(3431979..3433622) 1 NC_000962.3 Rv3068c, (MTCY22D7.13), len: 547 aa. Probable pgmA,phosphoglucomutase, highly similar to other phosphoglucomutases e.g. Q9L117|PGM from Streptomyces coelicolor (546 aa), FASTA scores: opt: 2569, E(): 2.8e-149, (71.4% identity in 545 aa overlap); Q9ABY5|CC0085 from Caulobacter crescentus (545 aa), FASTA scores: opt: 2465, E(): 6.2e-143, (70.4% identity in 541 aa overlap); P38569|PGMU_ACEXY|CELB from Acetobacter xylinum (555 aa),FASTA scores: opt: 2206, E(): 4e-127, (62.25% identity in 543 aa overlap); P74643|PGM|SLL0726 from Synechocystis sp. strain PCC 6803 (567 aa), FASTA scores: opt: 2168, E(): 8.5e-125, (60.0% identity in 550 aa overlap); P36938|PGMU_ECOLI|PGM|B0688 from Escherichia coli (546 aa),FASTA scores: opt: 2111, E(): 2.5e-121, (58.2% identity in 550 aa overlap). Also similar to other phosphomannomutases. Has phosphoglucomutase and phosphomannomutase signature (PS00710) and ATP/GTP-binding site motif A (P-loop) (PS00017). Belongs to the phosphohexose mutases family.; Probable phosphoglucomutase PgmA (glucose phosphomutase) (PGM) 3433622 pgmA 888826 pgmA Mycobacterium tuberculosis H37Rv Probable phosphoglucomutase PgmA (glucose phosphomutase) (PGM) NP_217584.1 3431979 R 83332 CDS NP_217585.1 15610206 888859 3433692..3434090 1 NC_000962.3 Rv3069, (MTCY22D7.12c), len: 132 aa. Probable conserved transmembrane protein, similar to several hypothetical and CRCB bacterial proteins e.g. Q9A6V2|CC1981 CRCB protein (see citation below; seems to be involved in camphor resistance and chromosome condensation, promoting or protecting chromosome folding) from Caulobacter crescentus (127 aa), FASTA scores: opt: 275, E(): 1.6e-11,(41.1% identity in 124 aa overlap); Q9FC39|SC4G1.10 putative integral membrane protein from Streptomyces coelicolor (154 aa), FASTA scores: opt: 258, E(): 2.5e-10,(42.15% identity in 121 aa overlap); Q9V0X2|PAB1925 CRCB protein (see citation below) from Pyrococcus abyssi (123 aa), FASTA scores: opt: 256, E(): 2.8e-10, (39.8% identity in 113 aa overlap); O59171|PH1502 hypothetical 13.6 KDA protein from Pyrococcus horikoshii (123 aa), FASTA scores: opt: 249, E(): 8.2e-10, (38.65% identity in 119 aa overlap); etc.; Probable conserved transmembrane protein 3434090 888859 Rv3069 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217585.1 3433692 D 83332 CDS NP_217586.1 15610207 888651 3434087..3434467 1 NC_000962.3 Rv3070, (MTCY22D7.11c), len: 126 aa. Probable conserved integral membrane protein, similar to several hypothetical and CRCB bacterial proteins e.g. Q9FC37|SC4G1.12 putative integral membrane protein from Streptomyces coelicolor (124 aa), FASTA scores: opt: 280,E(): 3.1e-11, (45.3% identity in 117 aa overlap); O25823|HP1225 conserved hypothetical integral membrane protein from Helicobacter pylori (Campylobacter pylori) (130 aa), FASTA scores: opt: 225, E(): 1e-07, (33.35% identity in 123 aa overlap); O07590|YHDU hypothetical 12.4 KDA protein from Bacillus subtilis (118 aa), FASTA scores: opt: 224, E(): 1.1e-07, (37.85% identity in 111 aa overlap); Q9KVS9|VC0060 CRCB protein (see Hu et al., 1996; seems involved in camphor resistance and chromosome condensation, promoting or protecting chromosome folding) from Vibrio cholera (126 aa), FASTA scores: opt: 221, E(): 1.8e-07, (33.35% identity in 126 aa overlap); etc.; Probable conserved integral membrane protein 3434467 888651 Rv3070 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217586.1 3434087 D 83332 CDS NP_217587.1 15610208 888649 3434464..3435573 1 NC_000962.3 Rv3071, (MTCY22D7.10c), len: 369 aa. Conserved hypothetical protein, weakly similar in N-terminus of Q9A4V0|CC2725 hypothetical protein CC2725 from Caulobacter crescentus (113 aa), FASTA scores: opt: 141, E(): 0.031,(27.6% identity in 105 aa overlap). C-terminal region also weakly similar to other hypothetical proteins e.g. Q9FC38|YG11_STRCO from Streptomyces coelicolor (114 aa),FASTA scores: opt: 151, E(): 0.007, (31.65% identity in 98 aa overlap).; hypothetical protein 3435573 888649 Rv3071 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217587.1 3434464 D 83332 CDS NP_217588.1 15610209 887175 complement(3435798..3436322) 1 NC_000962.3 Rv3072c, (MTCY22D7.09), len: 174 aa. Hypothetical protein, similar in part to O87779 hypothetical 18.1 KDA protein (fragment) from Mycobacterium paratuberculosis (166 aa), FASTA scores: opt: 238, E(): 2.5e-08, (42.6% identity in 108 aa overlap); Q9AH10 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa), FASTA scores: opt: 228, E(): 1.7e-07, (34.25% identity in 111 aa overlap); P71557|Y953_MYCTU|Rv0953c|MTCY10D7.21 possible oxidoreductase from Mycobacterium tuberculosis strain H37Rv (304 aa), FASTA scores: opt: 208, E(): 3.2e-06, (38.9% identity in 108 aa overlap); etc. N-terminal region similar to several proteins from Mycobacterium tuberculosis.; hypothetical protein 3436322 887175 Rv3072c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217588.1 3435798 R 83332 CDS NP_217589.1 15610210 888647 complement(3436329..3436685) 1 NC_000962.3 Rv3073c, (MTCY22D7.08), len: 118 aa. Conserved hypothetical protein, highly similar to other e.g. Q9F3D7|SC2H2.18 from Streptomyces coelicolor (119 aa),FASTA scores: opt: 399, E(): 2.5e-20, (53.05% identity in 115 aa overlap); Q9K4K9|SC5F8.15c from Streptomyces coelicolor (117 aa), FASTA scores: opt: 334, E(): 6e-16,(49.1% identity in 112 aa overlap); Q9HKD5|TA0666 from Thermoplasma acidophilum (134 aa), FASTA scores: opt: 334,E(): 6.7e-16, (42.35% identity in 111 aa overlap); BAB53507|MLL7394 from Rhizobium loti (Mesorhizobium loti) (120 aa), FASTA scores: opt: 309, E(): 3e-14, (43.65% identity in 110 aa overlap); etc.; hypothetical protein 3436685 888647 Rv3073c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217589.1 3436329 R 83332 CDS NP_217590.1 15610211 888027 3436779..3438053 1 NC_000962.3 Rv3074, (MTCY22D7.07c), len: 424 aa. Conserved hypothetical protein, highly similar but shorter (46 aa) to P71806|Rv1378c|MTCY02B12.12c hypothetical 51.3 KDA protein from Mycobacterium tuberculosis (475 aa), FASTA scores: opt: 2009, E(): 5.8e-113, (72.95% identity in 429 aa overlap); and also similar to other hypothetical mycobacterium proteins e.g. O33266|Rv0336|MTCY279.03 (503 aa), FASTA scores: opt: 337, E(): 7.5e-13, (28.6% identity in 381 aa overlap); O33360|Rv0515|MTCY20G10.05 (503 aa),FASTA scores: opt: 337, E(): 7.5e-13, (28.6% identity in 381 aa overlap); etc.; hypothetical protein 3438053 888027 Rv3074 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217590.1 3436779 D 83332 CDS NP_217591.1 15610212 887178 complement(3438050..3438973) 1 NC_000962.3 Rv3075c, (MTCY22D7.06), len: 307 aa. Conserved protein, with some similarity to Q9I562|PA0883 probable acyl-CoA lyase beta chain from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 408, E(): 9.2e-19, (35.15% identity in 273 aa overlap); Q9S2U9|SC4G6.02 putative citrate lyase beta chain from Streptomyces coelicolor (274 aa), FASTA scores: opt: 384, E(): 3.1e-17, (34.7% identity in 265 aa overlap); O06162|cite|Rv2498c|MTCY07A7.04c from Mycobacterium tuberculosis (273 aa), FASTA scores: opt: 349, E(): 5.1e-15, (35.2% identity in 264 aa overlap); etc. Several initiation codons possible, first one chosen.; hypothetical protein 3438973 887178 Rv3075c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217591.1 3438050 R 83332 CDS NP_217592.1 15610213 888026 3439072..3439548 1 NC_000962.3 Rv3076, (MTCY22D7.05c), len: 158 aa. Conserved hypothetical protein, weakly similar to Q9AK12|SC8D11.07 hypothetical 17.0 KDA protein from Streptomyces coelicolor (151 aa), FASTA scores: opt: 110, E(): 1.5, (25.5% identity in 145 aa overlap).; hypothetical protein 3439548 888026 Rv3076 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217592.1 3439072 D 83332 CDS YP_177923.1 57117053 887419 3439541..3441352 1 NC_000962.3 Rv3077, (MTCY22D7.04c), len: 603 aa. Possible hydrolase, with some similarity to variety of hydrolases (aryl- and steryl sulfatases principaly) e.g. Q45087|PEHA phosphonate monoester hydrolase from Burkholderia caryophylli (514 aa), FASTA scores: opt: 239, E(): 7.2e-07,(23.95% identity in 413 aa overlap); Q9I1E5|PA2333 probable sulfatase from Pseudomonas aeruginosa (538 aa), FASTA scores: opt: 231, E(): 2.3e-06, (28.1% identity in 516 aa overlap); P31447|YIDJ_ECOLI|B3678 putative sulfatase from Escherichia coli (497 aa), FASTA scores: opt: 222, E(): 7.4e-06, (27.7% identity in 390 aa overlap); etc. Note that previously known as atsF.; Possible hydrolase 3441352 887419 Rv3077 Mycobacterium tuberculosis H37Rv Possible hydrolase YP_177923.1 3439541 D 83332 CDS NP_217594.1 15610215 888891 3441353..3441754 1 NC_000962.3 Rv3078, (MTCY22D7.03c), len: 133 aa. Probable hab,hydroxylaminobenzene mutase (5.-.-.-) (see Davis et al.,2000), highly similar to two hydroxylaminobenzene mutases from Pseudomonas pseudoalcaligenes O52214|HABA (135 aa),FASTA scores: opt: 495, E(): 6.8e-25, (51.1% identity in 133 aa overlap); and O52216|HABB (164 aa), FASTA scores: opt: 479, E(): 8.2e-24, (51.9% identity in 133 aa overlap) (see Davis et al., 2000); and to Q9AH35|NBZB hydroxylaminobenzene mutase from Pseudomonas putida (164 aa), FASTA scores: opt: 476, E(): 1.3e-23, (51.8% identity in 133 aa overlap) (see Park & Kim 2000). Gene name according to Pseudomonas pseudoalcaligenes nomenclature. Also similarity with putative different membrane proteins involved in transport (protein predicted to be a transmembrane protein).; Probable hydroxylaminobenzene mutase Hab 3441754 hab 888891 hab Mycobacterium tuberculosis H37Rv Probable hydroxylaminobenzene mutase Hab NP_217594.1 3441353 D 83332 CDS NP_217595.1 15610216 888660 complement(3441770..3442597) 1 NC_000962.3 Rv3079c, (MTCY22D7.02), len: 275 aa. Conserved protein, similar to other hypothetical mycobacterium proteins e.g. P71557|Y953_MYCTU|Rv0953c|MTCY10D7.21 possible oxidoreductase from Mycobacterium tuberculosis strain H37Rv (282 aa), FASTA scores: opt: 668, E(): 2.4e-34, (40.55% identity in 281 aa overlap); O06216|Rv2161c|MTCY270.07 from Mycobacterium tuberculosis strain H37Rv (288 aa), FASTA scores: opt: 595, E(): 8.5e-30, (40.9% identity in 274 aa overlap); O87779 from Mycobacterium paratuberculosis (166 aa), FASTA scores: opt: 464, E(): 7.2e-22, (41.55% identity in 166 aa overlap); etc. Also some similarity to other proteins e.g. Q9AH10 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa), FASTA scores: opt: 401, E(): 9.6e-18, (30.2% identity in 288 aa overlap); Q9AE04|RIF17 RIF17 protein from Amycolatopsis mediterranei (356 aa),FASTA scores: opt: 298, E(): 2.8e-11, (35.0% identity in 203 aa overlap); AAK48081|MT3720 luciferase-related protein from Mycobacterium tuberculosis strain CDC1551 (395 aa),FASTA scores: opt: 223, E(): 1.4e-06, (29.4% identity in 211 aa overlap).; hypothetical protein 3442597 888660 Rv3079c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217595.1 3441770 R 83332 CDS NP_217596.1 15610217 888659 complement(3442656..3445988) 1 NC_000962.3 Rv3080c, (MTV013.01c-MTCY22D7.01), len: 1110 aa. pknK, serine/threonine protein kinase involved in transcriptional regulatory function (see citation below). Similar but shorter in N-terminus (approximately 300 residues) to others e.g. Q48411|ACOK transcriptional regulatory protein of aco ABCD operon from Klebsiella pneumoniae (921 aa), FASTA scores: opt: 886, E(): 7.6e-37,(27.75% identity in 829 aa overlap); Q9HX92|PA3921 probable transcriptional regulator from Pseudomonas aeruginosa (belongs to the LuxR/UhpA family of transcriptional regulators) (906 aa), FASTA scores: opt: 760, E(): 1.5e-30,(29.55% identity in 822 aa overlap); Q9I2X9|PA1760 probable transcriptional regulator from Pseudomonas aeruginosa (belongs to the LuxR/UhpA family of transcriptional regulators) (907 aa), FASTA scores: opt: 696, E(): 2.3e-27,(25.85% identity in 685 aa overlap); P06993|malt (alias BAB37683|ECS4260 and AAG58520|malt) positive regulator of MAL regulon from Escherichia coli strain O157:H7 (901 aa),FASTA scores: opt: 660, E(): 1.4e-25, (29.25% identity in 530 aa overlap); Q9KNF3|VCA0011 malt regulatory protein from Vibrio cholerae (belongs to the LuxR/UhpA family of transcriptional regulators) (921 aa), FASTA scores: opt: 626, E(): 7.2e-24, (25.8% identity in 659 aa overlap); etc. N-terminal region similar to N-terminus of serine/threonine kinases e.g. Q9KK90|PKMA serine/threonine kinase (similar to the Ser/Thr family of protein kinases) from Amycolatopsis mediterranei (589 aa), FASTA scores: opt: 545, E(): 5.7e-20, (34.45% identity in 334 aa overlap); Q9RPT5|AMK serine/threonine protein kinase homolog (similar to the Ser/Thr family of protein kinases) from Amycolatopsis mediterranei (606 aa), FASTA scores: opt: 537, E(): 1.5e-19, (35.55% identity in 346 aa overlap); Q9L0I0|PKAD protein serine/threonine kinase from Streptomyces coelicolor (599 aa), FASTA scores: opt: 520,E(): 1e-18, (36.1% identity in 324 aa overlap); etc. N-terminal part also similar to O53510|PKNL_MYCTU|Rv2176|MT2232|MTV021.09 probable serine/threonine-protein kinase from Mycobacterium tuberculosis strain H37Rv (399 aa), FASTA scores: opt: 511,E(): 2.1e-18, (35.15% identity in 313 aa overlap). Contains PS00107 Protein kinases ATP-binding region signature and PS00017 ATP/GTP-binding site motif A (P-loop). Contains Hank's kinase subdomain. First part of the protein seems belong to the Ser/Thr family of protein kinases, and second parts seems belongs to the LuxR/UhpA family of transcriptional regulators.; Serine/threonine-protein kinase transcriptional regulatory protein PknK (protein kinase K) (STPK K) 3445988 pknK 888659 pknK Mycobacterium tuberculosis H37Rv Serine/threonine-protein kinase transcriptional regulatory protein PknK (protein kinase K) (STPK K) NP_217596.1 3442656 R 83332 CDS NP_217597.1 15610218 888650 3446040..3447278 1 NC_000962.3 Rv3081, (MTV013.02), len: 412 aa. Conserved hypothetical protein. Second part of the protein (approximately residues 250-412) shares weak similarity with other hypothetical proteins e.g. Q9YEU3|APE0488 from Aeropyrum pernix (188 aa), FASTA scores: opt: 149, E(): 0.019, E(): 0.019, (29.5% identity in 173 aa overlap); and first part shares weak similarity with C-terminal part of Q9RVT9|DR0933 alpha-amlyase from Deinococcus radiodurans (644 aa), FASTA scores: opt: 127, E(): 1.4, (27.25% identity in 198 aa overlap). Equivalent to AAK47502|MT3166 hypothetical 48.3 KDA protein from Mycobacterium tuberculosis strain CDC1551 (436 aa) but shorter 24 aa in N-terminus. Contains PS00850 Glycine radical signature and possible helix-turn-helix motif at aa 53-74.; hypothetical protein 3447278 888650 Rv3081 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217597.1 3446040 D 83332 CDS NP_217598.1 15610219 888657 complement(3447404..3448426) 1 NC_000962.3 Rv3082c, (MT3167, MTV013.03c), len: 340 aa. VirS,transcriptional regulatory protein araC/xylS family,probably involved in virulence (see citations below). Similar to many transcriptional regulators araC/xylS family e.g. Q9HZ25|PA3215 probable transcriptional regulator (AraC/XylS family) from Pseudomonas aeruginosa (337 aa),FASTA scores: opt: 379, E(): 3e-17, (30.4% identity in 306 aa overlap); Q9Z3Y6|PHBR polyhydroxybutyrate transcriptional activator from Pseudomonas sp. 61-3 (379 aa), FASTA scores: opt: 336, E(): 2e-14, (26.35% identity in 334 aa overlap); P72171|ORUR|PA0831 ornithine utilization transcriptional regulator oruR from Pseudomonas aeruginosa (339 aa), FASTA scores: opt: 274, E(): 1.9e-10,(23.7% identity in 321 aa overlap); Q9ZFW7 virulence regulating homolog from Pseudomonas alcaligenes (346 aa),FASTA scores: opt: 262, E(): 1.2e-09, (24.5% identity in 339 aa overlap); etc. Also similar to O69703|Rv3736|MTV025.084 putative regulatory protein (AraC/XylS family) from Mycobacterium tuberculosis strain H37Rv (353 aa), FASTA scores: opt: 656, E(): 3.5e-35,(36.95% identity in 333 aa overlap). Has potential helix-turn-helix motif at positions 252-273. Belongs to the AraC/XylS family of transcriptional regulators. Substrate of PknK.; Virulence-regulating transcriptional regulator VirS (AraC/XylS family) 3448426 virS 888657 virS Mycobacterium tuberculosis H37Rv Virulence-regulating transcriptional regulator VirS (AraC/XylS family) NP_217598.1 3447404 R 83332 CDS NP_217599.1 15610220 888655 3448504..3449991 1 NC_000962.3 Rv3083, (MTV013.04), len: 495 aa. Probable monooxygenase, highly similar to other putative monooxygenases flavin-binding family e.g. AAK48336|MT3969 from Mycobacterium tuberculosis strain CDC1551 (489 aa),FASTA scores: opt: 1692, E(): 4.9e-98, (49.7% identity in 489 aa overlap); Q9A588|CC2569 from Caulobacter crescentus (498 aa), FASTA scores: opt: 1684, E(): 1.6e-97, (52.25% identity in 484 aa overlap); Q9APW3 from Pseudomonas aeruginosa (508 aa), FASTA scores: opt: 1603, E(): 1.8e-92,(49.8% identity in 484 aa overlap); etc.; Probable monooxygenase (hydroxylase) 3449991 888655 Rv3083 Mycobacterium tuberculosis H37Rv Probable monooxygenase (hydroxylase) NP_217599.1 3448504 D 83332 CDS NP_217600.1 15610221 888652 3449997..3450923 1 NC_000962.3 Rv3084, (MTV013.05), len: 308 aa. Probable lipR,N-Acetyl-hydrolase/esterase, similar to other e.g. Q01109|BAH_STRH from Streptomyces hygroscopicus (299 aa),FASTA scores: opt: 558, E(): 4.1e-26, (40.25% identity in 246 aa overlap); Q9X8J4|SCE9.22 from Streptomyces coelicolor (266 aa), FASTA scores: opt: 544, E(): 2.5e-25,(36.95% identity in 257 aa overlap); Q56171|DEA from Streptomyces viridochromogenes (299 aa), FASTA scores: opt: 532, E(): 1.4e-24, (38.6% identity in 254 aa overlap); etc. Also similar to O06350|LIPF|Rv3487c|MTCY13E12.41c (277 aa),FASTA score: opt: 291, E(): 8.5e-10, (28.5% identity in 239 aa overlap). May belong to the 'GDXG' family of lipolytic enzymes.; Probable acetyl-hydrolase/esterase LipR 3450923 lipR 888652 lipR Mycobacterium tuberculosis H37Rv Probable acetyl-hydrolase/esterase LipR NP_217600.1 3449997 D 83332 CDS NP_217601.1 15610222 888656 3450920..3451750 1 NC_000962.3 Rv3085, (MTV013.06), len: 276 aa. Probable short-chain dehydrogenase/reductase, similar to various oxidoreductases in the short chain dehydrogenases/reductases family e.g. Q9CC98|ML1094 short chain alcohol dehydrogenase from Mycobacterium leprae (277 aa), FASTA scores: opt: 1059, E(): 4.8e-56, (61.65% identity in 266 aa overlap); Q9I3H6|PA1537 probable short-chain dehydrogenase from Pseudomonas aeruginos (295 aa), FASTA scores: opt: 858, E(): 4.7e-44, (48.4% identity in 285 aa overlap); Q9CBP7|ML1740 possible short chain reductase from Mycobacterium leprae (312 aa), FASTA scores: opt: 500, E(): 1e-22, (36.6% identity in 257 aa overlap); etc. Also similar to mycobacterium proteins O50460|Rv1245c|MTV006.17c dehydrogenase similar to the short-chain dehydrogenases/reductases family (276 aa),FASTA scores: opt: 1200, E(): 1.9e-64, (65.2% identity in 273 aa overlap); and P95101|Rv3057c|MTCY22D7.24 hypothetical dehydrogenase (287 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 3451750 888656 Rv3085 Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_217601.1 3450920 D 83332 CDS NP_217602.1 15610223 888654 3451781..3452887 1 NC_000962.3 Rv3086, (MTV013.07), len: 368 aa. Probable adhD,zinc-type alcohol dehydrogenase, highly similar to many e.g. O69045 hypothetical alcohol dehydrogenase from Rhodococcus rhodochrous (370 aa), FASTA scores: opt: 1255,E(): 8.7e-68, (50.4% identity in 367 aa overlap); P25406|ADHB_UROHA alcohol dehydrogenase I-B from Uromastyx hardwickii (Indian spiny-tailed lizard) (375 aa), FASTA scores: opt: 787, E(): 8.2e-40, (35.9% identity in 373 aa overlap); P72324||ADHI_RHOSH alcohol dehydrogenase class III from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (376 aa), FASTA scores: opt: 787, E(): 8.3e-40, (35.1% identity in 379 aa overlap). Also highly similar to P71818|Rv0761c|MTCY369.06c hypothetical zinc-type alcohol dehydrogenase-like protein from Mycobacterium tuberculosis strain H37Rv (375 aa), FASTA scores: opt: 1186, E(): 1.2e-63, (47.3% identity in 368 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase. Possibly requires zinc for its activity.; Probable zinc-type alcohol dehydrogenase AdhD (aldehyde reductase) 3452887 adhD 888654 adhD Mycobacterium tuberculosis H37Rv Probable zinc-type alcohol dehydrogenase AdhD (aldehyde reductase) NP_217602.1 3451781 D 83332 CDS NP_217603.1 15610224 888653 3452925..3454343 1 NC_000962.3 Rv3087, (MTV013.08), len: 472 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to several Mycobacterium tuberculosis proteins e.g. MTCY08D5.16, MTCY28.26, MTCY493.29c. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa).; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 3454343 888653 Rv3087 Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_217603.1 3452925 D 83332 CDS NP_217604.1 15610225 888669 3454340..3455764 1 NC_000962.3 Rv3088, (MTV013.09), len: 474 aa. Putative tgs4,triacylglycerol synthase (See Daniel et al., 2004), similar to several Mycobacterium tuberculosis proteins e.g. MTCY31.23 (505 aa), MTCY13E12.34c (497 aa) and MTCY493.29c (459 aa). Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa).; Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs4 3455764 tgs4 888669 tgs4 Mycobacterium tuberculosis H37Rv Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs4 NP_217604.1 3454340 D 83332 CDS NP_217605.1 15610226 888666 3455761..3457272 1 NC_000962.3 Rv3089, (MTV013.10), len: 503 aa. Probable fadD13,Acyl-CoA Synthetase, similar to many e.g. MTCI28.06,MTCY08D5.09, MTCY06G11.08 from Mycobacterium tuberculosis strain H37Rv; and to Q9F7P5 predicted acid--CoA ligase FADD13 from uncultured proteobacterium EBAC31A08 (504 aa),FASTA scores: opt: 1126, E(): 2.4e-62, (38.85% identity in 502 aa overlap); Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. strain HR167 (491 aa), FASTA scores: opt: 1073, E(): 4.5e-59, (38.5% identity in 504 aa overlap); BAB49118|MLR1843 probable acid-CoA ligase from Rhizobium loti (Mesorhizobium loti) (495 aa), FASTA scores: opt: 937,E(): 1.2e-50, (36.2% identity in 503 aa overlap); Q9KZC1|SC6F7.21 probable long-chain-fatty-acid-CoA ligase from Streptomyces coelicolor (511 aa), FASTA scores: opt: 899, E(): 2.8e-48, (36.1% identity in 510 aa overlap); Q9A5P7|CC2400 putative acid-CoA ligase from Caulobacter crescentus (496 aa), FASTA scores: opt: 874, E(): 9.8e-47,(35.1% identity in 507 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature and PS00061 Short-chain alcohol dehydrogenase family signature.; Probable chain-fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase) 3457272 fadD13 888666 fadD13 Mycobacterium tuberculosis H37Rv Probable chain-fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase) NP_217605.1 3455761 D 83332 CDS NP_217606.1 15610227 888668 3458211..3459098 1 NC_000962.3 Rv3090, (MTCY164.01), len: 295 aa. Unknown Ala-,Val-rich protein. Hydrophobic stretch at N-terminus.; Unknown alanine and valine rich protein 3459098 888668 Rv3090 Mycobacterium tuberculosis H37Rv Unknown alanine and valine rich protein NP_217606.1 3458211 D 83332 CDS NP_217607.1 15610228 888665 3459116..3460807 1 NC_000962.3 Rv3091, (MTCY164.02), len: 563 aa. Conserved protein, similar in part to O60859 neuropathy target esterase from Homo sapiens (Human) (1327 aa), FASTA scores: opt: 177, E(): 0.0062, (30.65% identity in 173 aa overlap); and Q9I385|PA1640 hypothetical protein from Pseudomonas aeruginosa (345 aa), FASTA scores: opt: 152, E(): 0.069,(27.8% identity in 180 aa overlap).; hypothetical protein 3460807 888665 Rv3091 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217607.1 3459116 D 83332 CDS NP_217608.1 15610229 888667 complement(3460814..3461734) 1 NC_000962.3 Rv3092c, (MTCY164.03c), len: 306 aa. Probable conserved integral membrane protein, highly similar to Q9RUT5|DR1297 conserved hypothetical protein from Deinococcus radiodurans (311 aa), FASTA scores: opt: 941,E(): 9.8e-51, (55.65% identity in 309 aa overlap); Q9A8B8|CC1436 hypothetical protein from Caulobacter crescentus (314 aa), FASTA scores: opt: 791, E(): 1.6e-41,(46.9% identity in 305 aa overlap); and also highly similar to Q9I2N8|PA1857 hypothetical protein from Pseudomonas aeruginosa (307 aa), FASTA scores: opt: 373, E(): 8.1e-16,(40.8% identity in 321 aa overlap); BAB36119|ECS2696 putative methyl-independent mismatch repair protein from Escherichia coli strain O157:H7 (305 aa), FASTA scores: opt: 335, E(): 1.7e-13, (39.75% identity in 307 aa overlap).; Probable conserved integral membrane protein 3461734 888667 Rv3092c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217608.1 3460814 R 83332 CDS NP_217609.1 15610230 888663 complement(3461760..3462764) 1 NC_000962.3 Rv3093c, (MTCY164.04c), len: 334 aa. Hypothetical oxidoreductase, with some similarity with various oxidoreductases e.g. Q58929|mer|MJ1534 N5,N10-methylene tetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 300, E(): 1.1e-10,(24.1% identity in 324 aa overlap); and Q9ZA30|GRA-ORF29 putative FMN-dependent monooxygenase from Streptomyces violaceoruber (343 aa), FASTA scores: opt: 264, E(): 1.5e-08, (30.45% identity in 335 aa overlap); Q9CCV8|ML0348 possible coenzyme F420-dependent oxidoreductase from Mycobacterium leprae (350 aa), FASTA scores: opt: 220, E(): 6.4e-06, (26.5% identity in 328 aa overlap); etc.; Hypothetical oxidoreductase 3462764 888663 Rv3093c Mycobacterium tuberculosis H37Rv Hypothetical oxidoreductase NP_217609.1 3461760 R 83332 CDS NP_217610.1 15610231 888661 complement(3462761..3463891) 1 NC_000962.3 Rv3094c, (MTCY164.05c), len: 376 aa. Conserved hypothetical protein, some similarity with various proteins e.g. Q9RMR9|NRGC NRGC protein (corresponding gene seems regulated by NifA) from Bradyrhizobium japonicum (388 aa),FASTA scores: opt: 677, E(): 5.8e-35, (34.55% identity in 353 aa overlap); P26698|PIGM_RHOSO pigment protein from Rhodococcus sp. strain ATCC 21145 (387 aa), FASTA scores: opt: 480, E(): 1.2e-22, (28.7% identity in 376 aa overlap); Q9F0J3|NCNH hydroxylase from Streptomyces arenae (405 aa),FASTA scores: opt: 441, E(): 3.3e-20, (29.25% identity in 352 aa overlap); etc. Equivalent to AAK47516 from Mycobacterium tuberculosis strain CDC1551 (395 aa) but N-terminus shorter 19 aa.; hypothetical protein 3463891 888661 Rv3094c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217610.1 3462761 R 83332 CDS NP_217611.1 15610232 888679 3463973..3464449 1 NC_000962.3 Rv3095, (MTCY164.06), len: 158 aa. Possible regulatory protein, because contains possible helix-turn-helix motif at aa 39-61 (+4.83 SD). Similar to hypothetical proteins e.g. Q9I0C9|PA2713 from Pseudomonas aeruginosa (159 aa), FASTA scores: opt: 486, E(): 1.6e-25,(45.95% identity in 148 aa overlap); Q9AAF6|CC0645 from Caulobacter crescentus (188 aa), FASTA scores: opt: 479,E(): 5.3e-25, (45.75% identity in 153 aa overlap); Q9K408|2SCG61.07 from Streptomyces coelicolor (157 aa),FASTA scores: opt: 407, E(): 2.8e-20, (43.9% identity in 139 aa overlap); etc.; Hypothetical transcriptional regulatory protein 3464449 888679 Rv3095 Mycobacterium tuberculosis H37Rv Hypothetical transcriptional regulatory protein NP_217611.1 3463973 D 83332 CDS NP_217612.1 15610233 888678 3464547..3465686 1 NC_000962.3 Rv3096, (MTCY164.07), len: 379 aa. Hypothetical protein, with slight similarity to several proteins e.g. Q09671|OYEB_SCHPO|SPAC5H10.10 putative NADPH dehydrogenase C5H10.10 (old yellow enzyme homolog) from Schizosaccharomyces pombe (Fission yeast) (392 aa), FASTA scores: opt: 125, E(): 1.1, (25.45% identity in 165 aa overlap); and Q12603|XYNA_DICTH beta-1,4-xylanase (endo-1,4-beta-xylanase) from Dictyoglomus thermophilum (352 aa), FASTA scores: opt: 124, E(): 1.2, (25.65% identity in 195 aa overlap); etc. Contains glycosyl hydrolases family 5 signature (PS00659). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 3465686 888678 Rv3096 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217612.1 3464547 D 83332 CDS YP_177924.1 57117054 888677 complement(3465778..3467091) 1 NC_000962.3 Rv3097c, (MTCY164.08c), len: 437 aa. LipY,triacylglycerol lipase. Belongs to the hormone-sensitive lipase family (See Deb et al., 2006) and member of the M. tuberculosis PE-family PGRS subfamily of gly-rich proteins (see citation below); N-terminal part similar to N-terminus of M. tuberculosis PE-PGRS family members e.g. Q10637|Y03A_MYCTU hypothetical glycine-rich 49.6 kDa protein (603 aa). Other relatives include MTCY1A11.25c; MTCY21B4.13c; MTCY270.06; MTCY359.33; MTC1A11.04. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PE-PGRS family protein, triacylglycerol lipase LipY (esterase/lipase) (triglyceride lipase) (tributyrase) 3467091 lipY 888677 lipY Mycobacterium tuberculosis H37Rv PE-PGRS family protein, triacylglycerol lipase LipY (esterase/lipase) (triglyceride lipase) (tributyrase) YP_177924.1 3465778 R 83332 CDS NP_217614.1 15610235 888676 complement(3467210..3467662) 1 NC_000962.3 Rv3098c, (MTCY164.09c), len: 150 aa. Hypothetical unknown protein (shorter version of MTCY164.09c). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 3467662 888676 Rv3098c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217614.1 3467210 R 83332 CDS YP_007411812.1 448817939 14515872 3467606..3467926 1 NC_000962.3 Rv3098A, len: 106 aa. PemK-like protein.; PemK-like protein 3467926 14515872 Rv3098A Mycobacterium tuberculosis H37Rv PemK-like protein YP_007411812.1 3467606 D 83332 CDS NP_217615.1 15610236 888674 complement(3468413..3469264) 1 NC_000962.3 Rv3099c, (MTCY164.10c), len: 283 aa. Conserved protein, some similarity with hypothetical proteins e.g. Q9XA69|SCGD3.09 from Streptomyces coelicolor (274 aa),FASTA scores: opt: 384, E(): 1.8e-17, (32.7% identity in 269 aa overlap); and P71606|Y036_MYCTU|Rv0036c from Mycobacterium tuberculosis strain H37Rv (257 aa), FASTA scores: opt: 179, E(): 0.00024, (25.85% identity in 205 aa overlap).; hypothetical protein 3469264 888674 Rv3099c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217615.1 3468413 R 83332 CDS NP_217616.1 15610237 888675 complement(3469301..3469783) 1 NC_000962.3 Rv3100c, (MTCY164.11c), len: 160 aa. Probable smpB,small protein b related to several bacterial small protein b homologs e.g. O32881|SSRP_MYCLE|ML0671|MLCB1779.19c from Mycobacterium leprae (160 aa), FASTA scores: opt: 914, E(): 1.1e-52, (84.9% identity in 159 aa overlap); Q9L1S9|SMPB from Streptomyces coelicolor (159 aa), FASTA scores: opt: 568, E(): 3.3e-30, (55.15% identity in 145 aa overlap); O32230|SSRP_BACSU from Bacillus subtilis (156 aa), FASTA scores: opt: 511, E(): 1.7e-26, (47.05% identity in 153 aa overlap); etc. Belongs to the SSRP family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable SSRA-binding protein SmpB 3469783 smpB 888675 smpB Mycobacterium tuberculosis H37Rv Probable SSRA-binding protein SmpB NP_217616.1 3469301 R 83332 CDS NP_217617.1 15610238 888673 complement(3469786..3470679) 1 NC_000962.3 Rv3101c, (MTCY164.12c), len: 297 aa. Putative ftsX,cell division protein, septation component transport integral membrane protein ABC transporter (see citations below), equivalent to O32882|FTSX_MYCLE|ML0670|MLCB1779.20c cell division protein from Mycobacterium leprae (297 aa),FASTA scores: opt: 1597, E(): 9.2e-93, (80.8% identity in 297 aa overlap); and similar to others e.g. Q9L1S7|SCE59.27c from Streptomyces coelicolor (305 aa),FASTA scores: opt: 585, E(): 1.9e-29, (34.55% identity in 304 aa overlap); O34876|FTSX_BACSU from Bacillus subtilis (296 aa), FASTA scores: opt: 318, E(): 9.1e-13, (24.65% identity in 300 aa overlap); Q9K6X3|FTSX|BH3601 from Bacillus halodurans (298 aa), FASTA scores: opt: 290, E(): 5.2e-11, (22.75% identity in 299 aa overlap); etc. Belongs to the FTSX family.; Putative cell division protein FtsX (septation component-transport integral membrane protein ABC transporter) 3470679 ftsX 888673 ftsX Mycobacterium tuberculosis H37Rv Putative cell division protein FtsX (septation component-transport integral membrane protein ABC transporter) NP_217617.1 3469786 R 83332 CDS NP_217618.1 15610239 888672 complement(3470680..3471369) 1 NC_000962.3 Rv3102c, (MTCY164.13_2c), len: 229 aa. Putative ftsE, cell division protein, septation component transport ATP-binding protein ABC transporter (see citations below),equivalent to O32883|FTSE|ML0669 cell division ATP-binding protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 1384, E(): 2.4e-74, (91.7% identity in 229 aa overlap); and similar to Q9L1S6|FTSE from Streptomyces coelicolor (229 aa), FASTA scores: opt: 914, E(): 8.7e-47,(62.85% identity in 226 aa overlap); Q9A0S4|FTSE|SPY0644 from Streptococcus pyogenes (230 aa), FASTA scores: opt: 866, E(): 5.7e-44, (57.9% identity in 228 aa overlap); Q9CGX0|FTSE from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (230 aa), FASTA scores: opt: 792,E(): 1.3e-39, (52.2% identity in 228 aa overlap); etc. Other relatives from Mycobacterium tuberculosis include: MTCY253.24; MTCY16B7.10; MTCY9C4.04c; MTCY50.01; MTCY05A6.09c; MTCY04C12.31. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and ABC transporters family signature (PS00211). Belongs to the ATP-binding transport protein family (ABC transporters).; Putative cell division ATP-binding protein FtsE (septation component-transport ATP-binding protein ABC transporter) 3471369 ftsE 888672 ftsE Mycobacterium tuberculosis H37Rv Putative cell division ATP-binding protein FtsE (septation component-transport ATP-binding protein ABC transporter) NP_217618.1 3470680 R 83332 CDS NP_217619.1 15610240 888798 complement(3471413..3471850) 1 NC_000962.3 Rv3103c, (MTCY164.13c), len: 145 aa. Hypothetical unknown pro-rich protein, with some similarity to Proline-rich proteins e.g. Q39789 proline-rich cell wall protein from Gossypium hirsutum (Upland cotton) (214 aa),FASTA scores: opt: 267, E(): 0.00014, (40% identity in 110 aa overlap). Equivalent to AAK47525 from M. tuberculosis strain CDC1551 (158 aa) but shorter 13 aa.; Hypothetical proline-rich protein 3471850 888798 Rv3103c Mycobacterium tuberculosis H37Rv Hypothetical proline-rich protein NP_217619.1 3471413 R 83332 CDS NP_217620.1 15610241 888685 complement(3471852..3472778) 1 NC_000962.3 Rv3104c, (MTCY164.14c), len: 308 aa. Possible conserved transmembrane protein, with some similarity to hypthetical proteins e.g. Q9L1X9|SC8E4A.26 putative membrane protein from Streptomyces coelicolor (408 aa),FASTA scores: opt: 514, E(): 4.3e-25, (35.2% identity in 287 aa overlap); Q9XA89|CF43A.26c hypothetical 36.1 KDA protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 482, E(): 3.7e-23, (34.9% identity in 301 aa overlap); Q55987|SLR0765 hypothetical 68.9 KDA protein from Synechocystis sp. strain PCC 6803 (617 aa), FASTA scores: opt: 429, E(): 1.3e-19, (30.6% identity in 278 aa overlap); etc.; Possible conserved transmembrane protein 3472778 888685 Rv3104c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_217620.1 3471852 R 83332 CDS NP_217621.1 15610242 888820 complement(3472768..3473904) 1 NC_000962.3 Rv3105c, (MTCY164.15c), len: 378 aa. Probable prfB,peptide chain release factor 2, equivalent to O32885|RF2_MYCLE|ML0667|MLCB1779.24c from Mycobacterium leprae, FASTA scores: opt: 2197, E(): 1.8e-126, (90.05% identity in 372 aa overlap); and also similar to other peptide chain release factors e.g. Q9L1S3|PRFB from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1674,E(): 1.2e-94, (69.3% identity in 365 aa overlap); O67695|RF2_AQUAE|PRFB|AQ_1840 from Aquifex aeolicus (373 aa), FASTA scores: opt: 1082, E(): 1.3e-58, (44.45% identity in 369 aa overlap); P28367|RF2_BACSU from B. subtilis (366 aa), FASTA scores: opt: 1030, E(): 1.9e-55,(44.0% identity in 359 aa overlap); etc. Also related to Q10605|MTCY373.19|RF1_MYCTU|Rv1299|MT1338 peptide chain release factor 1 (rf-1) (357 aa), FASTA scores: opt: 646,E(): 1.1e-34, (38.6% identity in 350 aa overlap). Contains prokaryotic-type class I peptide chain release factors signature (PS00745). Belongs to the prokaryotic and mitochondrial release factors family.; Probable peptide chain release factor 2 PrfB (RF-2) 3473904 prfB 888820 prfB Mycobacterium tuberculosis H37Rv Probable peptide chain release factor 2 PrfB (RF-2) NP_217621.1 3472768 R 83332 CDS NP_217622.1 15610243 888839 3474007..3475377 1 NC_000962.3 Rv3106, (MTCY164.16), len: 456 aa. FprA,NADPH:adrenodoxin oxidoreductase (NADPH-ferredoxin reductase) (see citations below), equivalent to O32886|MLCB1779.25|FPRA|ML0666 from Mycobacterium leprae (456 aa), FASTA scores: opt: 2505, E(): 1.2e-142, (81,05% identity in 459 aa overlap); also similar to other NADPH:adrenodoxin oxidoreductases e.g. Q9RX19|DR0496 from Deinococcus radiodurans (479 aa), FASTA scores: opt: 1331,E(): 2.6e-72, (48.9% identity in 454 aa overlap); Q9RK35|SCF15.02 from Streptomyces coelicolor (454 aa),FASTA scores: opt: 1102, E(): 1.3e-58, (41.35% identity in 462 aa overlap); P82861 from Salvelinus fontinalis (Brook trout) (498 aa), FASTA scores: opt: 827, E(): 4e-42, (41.3% identity in 460 aa overlap); Q9V3T9|ADRO_DROME from Drosophila melanogaster (Fruit fly) (466 aa), FASTA scores: opt: 790, E(): 6.3e-40, (39.45% identity in 459 aa overlap); etc. Also similar to Q10547|FPRB|Rv0886|MT0909|MTCY31.14 from Mycobacterium tuberculosis strain H37Rv (575 aa), FASTA scores: opt: 894,E(): 4.4e-46, (42.05% identity in 459 aa overlap). Cofactor: FAD; NADPH:adrenodoxin oxidoreductase FprA (NADPH-ferredoxin reductase) 3475377 fprA 888839 fprA Mycobacterium tuberculosis H37Rv NADPH:adrenodoxin oxidoreductase FprA (NADPH-ferredoxin reductase) NP_217622.1 3474007 D 83332 CDS NP_217623.1 15610244 887657 complement(3475378..3476961) 1 NC_000962.3 Rv3107c, (MTCY164.17c), len: 527 aa. Possible agpS,alkyl-dihydroxyacetonephosphate synthase, similar to others and some various enzymes e.g. AAK46595|MT2311 putative alkyl-dihydroxyacetonephosphate synthase from Mycobacterium tuberculosis strain CDC1551 (529 aa), FASTA scores: opt: 1052, E(): 2.1e-58, (37.1% identity in 542 aa overlap); Q9RJ97|SCF91.28c putative flavoprotein from Streptomyces coelicolor (530 aa), FASTA scores: opt: 972, E(): 2.2e-53,(36.2% identity in 544 aa overlap); O96759|ADAS_DICDI alkyldihydroxyacetonephosphate synthase from Dictyostelium discoideum (Slime mold) (611 aa), FASTA scores: opt: 617,E(): 4.5e-31, (33.95% identity in 480 aa overlap); O97157|ADAS_TRYBB alkyldihydroxyacetonephosphate synthase from Trypanosoma brucei (613 aa), FASTA scores: opt: 567,E(): 6.2e-28, (29.15% identity in 521 aa overlap); etc. Also similar to O53525|Rv2251|MTV022.01 hypothetical 49.8 KDA protein from Mycobacterium tuberculosis strain H37Rv (475 aa), FASTA scores: opt: 1019, E(): 2.3e-56, (38.6% identity in 487 aa overlap). Belongs to the FAD-binding oxidoreductase/transferase family 4. Cofactor: FAD (by similarity).; Possible alkyldihydroxyacetonephosphate synthase AgpS (alkyl-DHAP synthase) (alkylglycerone-phosphate synthase) 3476961 agpS 887657 agpS Mycobacterium tuberculosis H37Rv Possible alkyldihydroxyacetonephosphate synthase AgpS (alkyl-DHAP synthase) (alkylglycerone-phosphate synthase) NP_217623.1 3475378 R 83332 CDS NP_217624.1 15610245 888686 3477060..3477500 1 NC_000962.3 Rv3108, (MTCY164.18), len: 146 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3477500 888686 Rv3108 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217624.1 3477060 D 83332 CDS YP_177925.1 57117055 888836 3477649..3478728 1 NC_000962.3 Rv3109, (MTCY164.19), len: 359 aa. Probable moaA1,molybdenum cofactor biosynthesis protein, highly similar to others e.g. P39757|MOAA_BACSU|NARA|NARAB from Bacillus subtilis (341 aa), FASTA scores: opt: 810, E(): 6.2e-44,(39.75% identity in 327 aa overlap); O67929|MOAA_AQUAE|AQ_2183 from Aquifex aeolicus (320 aa),FASTA scores: opt: 794, E(): 6e-43, (40.55% identity in 323 aa overlap); Q9ZIM6|MOAA_STACA from Staphylococcus carnosus (340 aa), FASTA scores: opt: 783, E(): 3.2e-42, (38.65% identity in 326 aa overlap); etc. Also highly similar to O53143|MOAA3|MOA3_MYCTU|MT3427 molybdenum cofactor biosynthesis protein A 3 from Mycobacterium tuberculosis strain F4 (378 aa), FASTA scores: opt: 1762, E(): 4.7e-104,(74.3% identity in 350 aa overlap); and similar to O53881|MOA2_MYCTU|MOAA2|Rv0869c|MT0892|MTV043.62 molybdenum cofactor biosynthesis protein A 2 from Mycobacterium tuberculosis strain H37Rv (360 aa), FASTA scores: opt: 657,E(): 3e-34, (36.55% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family. Note that previously known as moaA. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable molybdenum cofactor biosynthesis protein A MoaA1 3478728 moaA1 888836 moaA1 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein A MoaA1 YP_177925.1 3477649 D 83332 CDS YP_177926.1 57117056 888683 3478779..3479174 1 NC_000962.3 Rv3110, (MTCY164.20), len: 131 aa. Probable moaB1,pterin-4-alpha-carbinolamine dehydratase, similar to others e.g. P73790|SSL2296 from Synechocystis sp. strain PCC 6803 (96 aa), FASTA scores: opt: 195, E(): 6.2e-07, (35.4% identity in 96 aa overlap); Q9PAB4|PHS_XYLFA|XF2604 from Xylella fastidiosa (116 aa), FASTA scores: opt: 187, E(): 2.6e-06, (36.25% identity in 102 aa overlap); AAK42360|Q97WM6|PHS_SULSO|SSO2187 from Sulfolobus solfataricus (114 aa), FASTA scores: opt: 177, E(): 1.3e-05, (34.6% identity in 78 aa overlap); etc. Also highly similar to AAK47768|MT3426 pterin-4-alpha-carbinolamine dehydratase from Mycobacterium tuberculosis CDC1551 (124 aa), FASTA scores: opt: 383, E(): 7.7e-20, (50.0% identity in 110 aa overlap). Belongs to the pterin-4-alpha-carbinolamine dehydratase family. Note that previously known as moaB. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable pterin-4-alpha-carbinolamine dehydratase MoaB1 (PHS) (4-alpha-hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) 3479174 moaB1 888683 moaB1 Mycobacterium tuberculosis H37Rv Probable pterin-4-alpha-carbinolamine dehydratase MoaB1 (PHS) (4-alpha-hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) YP_177926.1 3478779 D 83332 CDS YP_177927.1 57117057 888680 3479171..3479683 1 NC_000962.3 Rv3111, (MTCY164.21), len: 170 aa. Probable moaC1,molybdopterin cofactor biosynthesis protein, highly similar to others e.g. Q9HX95|MOAC|PA3918 from Pseudomonas aeruginosa (160 aa), FASTA scores: opt: 576, E(): 2.2e-29,(62.1% identity in 153 aa overlap); Q9ZFA6|MOAC from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (159 aa), FASTA scores: opt: 541, E(): 3.4e-27, (59.85% identity in 157 aa overlap); BAB48171|MLR0616 from Rhizobium loti (Mesorhizobium loti) (160 aa), FASTA scores: opt: 531, E(): 1.5e-26, (58.75% identity in 160 aa overlap); P30747|MOAC_ECOLI|CHLA3|B0783 from Escherichia coli strain K12 (160 aa), FASTA scores: opt: 527, E(): 2.6e-26, (58.5% identity in 159 aa overlap); etc. Also highly similar to O53376|MOAC3|Rv3324c|MTV016.24c putative molybdenum cofactor biosynthesis protein C 3 from Mycobacterium tuberculosis (177 aa), FASTA scores: opt: 738, E(): 1.7e-39, (71.5% identity in 165 aa overlap); AAK47767|MT3425 molybdopterin cofactor biosynthesis protein C from Mycobacterium tuberculosis strain CDC1551 (184 aa),FASTA scores: opt: 734, E(): 3.1e-39, (71.8% identity in 163 aa overlap); and Rv0864|MOAC2|MTV043.57 putative molybdenum cofactor biosynthesis protein C 2 (167 aa). Note that previously known as moaC. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable molybdenum cofactor biosynthesis protein C MoaC1 3479683 moaC1 888680 moaC1 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein C MoaC1 YP_177927.1 3479171 D 83332 CDS YP_177928.1 57117058 888897 3479700..3479951 1 NC_000962.3 Rv3112, (MTCY164.22), len: 83 aa. Probable moaD1,molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to others e.g. Q9HJF0|TA1019 from Thermoplasma acidophilum (85 aa), FASTA scores: opt: 144, E(): 0.0012, (31.7% identity in 82 aa overlap); BAB59710|TVG0556526 from Thermoplasma volcanium (90 aa), FASTA scores: opt: 144, E(): 0.0012, (31.7% identity in 82 aa overlap); P30748|MOAD_ECOLI|CHLA4|CHLM|B0784 from Escherichia coli strain K12 (81 aa), FASTA scores: opt: 116, E(): 0.11,(36.9% identity in 84 aa overlap); etc. N-terminus also highly similar to to O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 333, E(): 2e-16, (65.05% identity in 83 aa overlap); and some similarity with Rv0868c|MTV043.61c|MOAD2 putative molybdenum cofactor biosynthesis protein D 2 (92 aa). Note that previously known as moaD. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable molybdenum cofactor biosynthesis protein D MoaD1 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) 3479951 moaD1 888897 moaD1 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein D MoaD1 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) YP_177928.1 3479700 D 83332 CDS NP_217629.1 15610250 888781 3480074..3480742 1 NC_000962.3 Rv3113, (MTCY164.23), len: 222 aa. Possible phosphatase, with weak similarity to other phosphatases e.g. Q9KYY0|SCE33.02c from Streptomyces coelicolor (223 aa), FASTA scores: opt: 368, E(): 1.2e-16, (32.9% identity in 222 aa overlap); and Q55039|GPH_SYNP7|CBBZ phosphoglycolate phosphatase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (212 aa), FASTA scores: opt: 176, E(): 0.00025, (24.7% identity in 182 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible phosphatase 3480742 888781 Rv3113 Mycobacterium tuberculosis H37Rv Possible phosphatase NP_217629.1 3480074 D 83332 CDS NP_217630.1 15610251 888779 3480759..3481289 1 NC_000962.3 Rv3114, (MTCY164.24), len: 176 aa. Conserved hypothetical protein, with some similarity to Q9F9W7 cytosine deaminase from Bifidobacterium longum (143 aa),FASTA scores: opt: 207, E(): 2.2e-07, (37.05% identity in 108 aa overlap); and Q9RV23|DR1207 cell cycle protein MESJ,putative/cytosine deaminase-related protein from Deinococcus radiodurans (600 aa), FASTA scores: opt: 212,E(): 3.5e-07, (33.35% identity in 177 aa overlap). Equivalent to AAK47536|MT3196 cytidine and deoxycytidylate deaminase family protein from Mycobacterium tuberculosis strain CDC1551 (187 aa) but shorter 11 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3481289 888779 Rv3114 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217630.1 3480759 D 83332 CDS NP_217631.1 15610252 888790 3481451..3482698 1 NC_000962.3 Rv3115, (MTCY164.25), len: 415 aa. Probable IS1081 transposase, similar to others. Has transposases, mutator family, signature (PS01007). Other copies are MTCY10G2.02c,MTCY441.35, MTCY77.03c. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable transposase 3482698 888790 Rv3115 Mycobacterium tuberculosis H37Rv Probable transposase NP_217631.1 3481451 D 83332 CDS YP_177929.1 57117059 888808 3482776..3483945 1 NC_000962.3 Rv3116, (MTCY164.26), len: 389 aa. Probable moeB2,molybdopterin cofactor biosynthesis protein, equivalent to Q9CCG8|MOEZ|ML0817 protein probably involved in molybdopterin biosynthesis from Mycobacterium leprae (395 aa), FASTA scores: opt: 1433, E(): 8e-80, (57.8% identity in 384 aa overlap). Very similar to members of the HESA/MOEB/THIF family e.g. Q9FCL0|2SC3B6.02 putative sulfurylase from Streptomyces coelicolor (392 aa), FASTA scores: opt: 1562, E(): 1.1e-87, (58.15% identity in 380 aa overlap); Q9XC37|PDTORFF MOEB-like protein (putative sulfurylase) from Pseudomonas stutzeri (Pseudomonas perfectomarina) (391 aa), FASTA scores: opt: 1311, E(): 2.1e-72, (52.4% identity in 395 aa overlap); O54307|MPT|MOEB MPT-synthase sulfurylase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (391 aa), FASTA scores: opt: 1238, E(): 5.7e-68, (51.4% identity in 393 aa overlap); P74344|MOEB|SLL1536 molybdopterin biosynthesis MOEB protein from Synechocystis sp. strain PCC 6803 (392 aa), FASTA scores: opt: 1212, E(): 2.2e-66, (46.5% identity in 398 aa overlap); etc. Also highly similar to O05860|MTCY07D11.20|MOEB1|Rv3206c putative molybdenum cofactor biosynthesis protein from Mycobacterium tuberculosis strain H37Rv (392 aa), FASTA scores: opt: 1445, E(): 1.5e-80, (56.25% identity in 400 aa overlap). Belongs to the HesA /MoeB/ThiF family. Note that previously known as moeB. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) 3483945 moeB2 888808 moeB2 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) YP_177929.1 3482776 D 83332 CDS NP_217633.1 15610254 888802 3483974..3484807 1 NC_000962.3 Rv3117, (MTCY164.27, MT3199, O05793), len: 277 aa. Probable cysA3 (alternate gene name: sseC3), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER|CYSA from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa), FASTA scores: opt: 1442, E(): 1.7e-84, (75.55% identity in 274 aa overlap); Q9RXT9DR0217|DR0217 from Deinococcus radiodurans (286 aa), FASTA scores: opt: 1046,E(): 2.6e-59, (53.8% identity in 275 aa overlap); Q9HMT7|TSSA|VNG2393G from Halobacterium sp. strain NRC-1 (293 aa), FASTA scores: opt: 1030, E(): 2.7e-58, (56.1% identity in 278 aa overlap); Q9Y8N8|APE2595 from Aeropyrum pernix (218 aa), FASTA scores: opt: 808, E(): 2.7e-44,(53.5% identity in 215 aa overlap); etc. Identical second copy present as Rv0815c|AL022004|MTV043.07c|MT0837|O05793|cysA2 (277 aa) (100.0% identity in 277 aa overlap). Also shows some similarity to P96888|THT2_MYCTU|SSEA|Rv3283|MT3382|MTCY71.23 putative thiosulfate sulfurtransferase from Mycobacterium tuberculosis (297 aa), FASTA scores: opt: 955, E(): 1.6e-53, (50.2% identity in 271 aa overlap); and Q59570|THT3_MYCTU|SSEB|Rv2291|MT2348|MTCY339.19c putative thiosulfate sulfurtransferase from Mycobacterium tuberculosis (284 aa), FASTA scores: E(): 1.4e-14, (26.7% identity in 292 aa overlap). Contains rhodanese active site and C-terminal signatures (PS00380, PS00683). Belongs to the rhodanese family. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable thiosulfate sulfurtransferase CysA3 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) 3484807 cysA3 888802 cysA3 Mycobacterium tuberculosis H37Rv Probable thiosulfate sulfurtransferase CysA3 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) NP_217633.1 3483974 D 83332 CDS YP_177930.1 57117060 888809 3484809..3485111 1 NC_000962.3 Rv3118, (MTCY164.28, O05794), len: 100 aa. SseC1,conserved hypothetical protein, equivalent to Q9CBC7|ML2199 hypothetical protein from Mycobacterium leprae (100 aa),FASTA scores: opt: 545, E(): 3.1e-30, (84.0% identity in 10 aa overlap). Also similar to hypothetical proteins e.g. Q50035 from Saccharopolyspora erythraea (Streptomyces erythraeus) (101 aa), FASTA scores: opt: 345, E(): 9.7e-17,(57.15% identity in 98 aa overlap); and Q9K4H3|SCD66.02 from Streptomyces coelicolor (95 aa), FASTA scores: opt: 249, E(): 2.8e-10, (48.5% identity in 99 aa overlap). Some weak similarity with Q9ZB84|PCAG protocatechuate 3,4-dioxygenase alpha-subunit from Pseudomonas marginata (196 aa), FASTA scores: opt: 109, E(): 1.4, (31.3% identity in 83 aa overlap); and other bacterial proteins. Identical second copy present as Rv0814c|AL022004|MTV043.06c|SSEC2 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap). Note that previously known as sseC. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Conserved hypothetical protein SseC1 3485111 sseC1 888809 sseC1 Mycobacterium tuberculosis H37Rv Conserved hypothetical protein SseC1 YP_177930.1 3484809 D 83332 CDS YP_177931.1 57117061 888811 3485132..3485575 1 NC_000962.3 Rv3119, (MTCY164.29), len: 147 aa. Probable moaE1,molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), highly similar to others e.g. O31705|MOAE from Bacillus subtilis (157 aa), FASTA scores: opt: 390, E(): 8.6e-19, (43.95% identity in 132 aa overlap); Q9K8I7|MOAE|BH3019 from Bacillus halodurans (156 aa), FASTA scores: opt: 369, E(): 2e-17, (42.4% identity in 132 aa overlap); P30749|MOAE_ECOLI|CHLA5|B0785 from Escherichia coli strain K12 (149 aa), FASTA scores: opt: 312, E(): 1.1e-13, (38.45% identity in 130 aa overlap); etc. Also highly similar (but shorter 74 aa) to O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 733, E(): 3.9e-41, (76.2% identity in 143 aa overlap); and highly similar to O53878|MOAE2|Rv0866|MTV043.59 putative molybdopterin synthase large subunit from Mycobacterium tuberculosis (141 aa), FASTA scores: opt: 321, E(): 2.6e-14, (40.9% identity in 132 aa overlap). Note that previously known as moaE. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable molybdenum cofactor biosynthesis protein E MoaE1 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) 3485575 moaE1 888811 moaE1 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein E MoaE1 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) YP_177931.1 3485132 D 83332 CDS NP_217636.1 15610257 888828 3485572..3486174 1 NC_000962.3 Rv3120, (MTCY164.30), len: 200 aa. Conserved hypothetical protein, with weak similarity to several hypothetical proteins and many N-methyl transferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor A3(2) (208 aa), FASTA scores: opt: 177, E(): 0.00011, (34.6% identity in 130 aa overlap); Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 147,E(): 0.011, (31.3% identity in 166 aa overlap); BAB52127|MLL5735 probable methyltransferase from Rhizobium loti (Mesorhizobium loti) (247 aa), FASTA scores: opt: 133,E(): 0.11, (29.75% identity in 158 aa overlap). Highly similar to O53374|Rv3322c|MTV016.22c possible methyltransferase from Mycobacterium tuberculosis strain H37Rv (204 aa), FASTA scores: opt: 691, E(): 1.1e-38,(57.0% identity in 200 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3486174 888828 Rv3120 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217636.1 3485572 D 83332 CDS NP_217637.1 15610258 887409 3486509..3487711 1 NC_000962.3 Rv3121, (MTCY164.31), len: 400 aa. Probable cyp141,cytochrome P-450 integral membrane protein, similar to other cytochrome P450-dependent oxidases e.g. Q9X5P9|CYP107N1 from Streptomyces lavendulae (410 aa),FASTA scores: opt: 825, E(): 3.1e-42, (33.35% identity in 393 aa overlap); Q59819|OLEP|CYP107D1 from Streptomyces antibioticus (407 aa), FASTA scores: opt: 812, E(): 1.9e-41, (34.85% identity in 396 aa overlap); O32460|CYP107M1 from Actinomadura hibisca (411 aa), FASTA scores: opt: 713, E(): 1.6e-35, (31.05% identity in 396 aa overlap); P55544|CPXP_RHISN|CYP112A|Y4LD from Rhizobium sp. strain NGR234 (400 aa), FASTA scores: opt: 688, E(): 5.1e-34, (33.0% identity in 406 aa overlap); etc. Also similar to MTCY339.44c, MTCY369.22, MTCY50.26, MTCY03C7.11,MTCY339.34c, MTCY339.42, MTCY369.11c. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable cytochrome P450 141 Cyp141 3487711 cyp141 887409 cyp141 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 141 Cyp141 NP_217637.1 3486509 D 83332 CDS NP_217638.1 15610259 888890 3488089..3488559 1 NC_000962.3 Rv3122, (MTCY164.32), len: 156 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3488559 888890 Rv3122 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217638.1 3488089 D 83332 CDS NP_217639.1 15610260 888823 3488569..3489063 1 NC_000962.3 Rv3123, (MTCY164.33), len: 164 aa. Hypothetical unknown protein, but N-terminus shares weak similarity with N-terminal part of O93439|CMESO-1 BHLH transcription factor from Gallus gallus (Chicken) (287 aa), FASTA scores: opt: 129, E(): 0.81, (38.75% identity in 80 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Hypothetical protein 3489063 888823 Rv3123 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217639.1 3488569 D 83332 CDS NP_217640.1 15610261 888825 3489506..3490375 1 NC_000962.3 Rv3124, (MTCY164.34), len: 289 aa. MoaR1,transcriptional regulatory protein, similar to many Streptomyces and Mycobacterium tuberculosis regulatory proteins e.g. Q11052|YC67_MYCTU|Rv1267c|MT1305|MTCY50.15 from Mycobacterium tuberculosis strain H37Rv (388 aa),FASTA scores: opt: 963, E(): 2e-56, (55.15% identity in 252 aa overlap); O53145 from Mycobacterium tuberculosis (381 aa); P71484|EMBR from Mycobacterium avium (384 aa), FASTA scores: opt: 859, E(): 1.5e-49, (52.2% identity in 249 aa overlap); Q9XCC3|TYLT from Streptomyces fradiae (404 aa),FASTA scores: opt: 462, E(): 3.1e-23, (35.05% identity in 254 aa overlap); Q9XCC4|TYLS from Streptomyces fradiae (277 aa), FASTA scores: opt: 456, E(): 5.6e-23, (33.45% identity in 269 aa overlap); etc. Start chosen by similarity,alternative possible (see AAK47548 from Mycobacterium tuberculosis strain CDC1551, longer N-terminus (311 aa)). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Transcriptional regulatory protein MoaR1 3490375 moaR1 888825 moaR1 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein MoaR1 NP_217640.1 3489506 D 83332 CDS YP_177932.1 57117062 888892 complement(3490476..3491651) 1 NC_000962.3 Rv3125c, (MTCY164.35c), len: 391 aa. PPE49, Member of the Mycobacterium tuberculosis PPE family, similar to other e.g. P95247|Rv2352c|MTCY98.21c (391 aa), FASTA scores: opt: 1576, E(): 3.8e-72, (62.55% identity in 398 aa overlap), MTCY98.0029c, MTCY03A2.22c, MTCY10G2.10,MTCY02B10.25c, MTCI364.08, M TCY21C12.09c, MTCY48.17. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; PPE family protein PPE49 3491651 PPE49 888892 PPE49 Mycobacterium tuberculosis H37Rv PPE family protein PPE49 YP_177932.1 3490476 R 83332 CDS NP_217642.1 15610263 888806 complement(3491808..3492122) 1 NC_000962.3 Rv3126c, (MTCY164.36c), unknown, len: 104 aa. Hypothetical unknown protein. Shortened version of MTCY164.36c, avoiding overlap. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3492122 888806 Rv3126c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217642.1 3491808 R 83332 CDS NP_217643.1 15610264 888850 3492147..3493181 1 NC_000962.3 Rv3127, (MTCY164.37), len: 344 aa. Conserved protein, highly similar to Mycobacterium tuberculosis protein O53476|Rv2032|MTV018.19 (331 aa), FASTA scores: opt: 1212, E(): 6e-69, (56.7% identity in 321 aa overlap),and also similar to P95195|MTCY03A2.27c (332 aa), FASTA scores: opt: 521, E(): 1.6e-25; (35.0% identity in 326 aa overlap). Some similarity to C-terminal half of hypothetical Mycobacterium tuberculosis proteins. Predicted possible vaccine candidate (See Zvi et al., 2008).; hypothetical protein 3493181 888850 Rv3127 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217643.1 3492147 D 83332 CDS YP_177933.1 57117063 888901 3494660..3494992 1 NC_000962.3 Rv3129, (MTCY164.40), len: 110 aa. Conserved hypothetical protein, with some similarity to various hypothetical proteins from Streptomyces coelicolor e.g. Q9RI34|SCJ12.26 hypothetical 14.5 KDA protein (137 aa),FASTA scores: opt: 141, E(): 0.0016, (39.3% identity in 84 aa overlap); Q9RI49|SCJ12.09c hypothetical 15.8 KDA protein (146 aa), FASTA scores: opt: 141, E(): 0.0017, (38.05% identity in 92 aa overlap); Q9RJ05|SCJ1.09C possible DNA-binding protein (233 aa), FASTA scores: opt: 140, E(): 0.0029, (34.85% identity in 89 aa overlap); Q9XA48|SCGD3.31c putative branched-chain alpha keto acid dehydrogenase E1 beta subunit (334 aa); etc.; hypothetical protein 3494992 888901 Rv3129 Mycobacterium tuberculosis H37Rv hypothetical protein YP_177933.1 3494660 D 83332 CDS NP_217646.1 15610266 888841 complement(3494975..3496366) 1 NC_000962.3 Rv3130c, (MTCY03A2.28, MTCY164.41c), len: 463 aa. tgs1, triacylglycerol synthase (See Daniel et al., 2004; Sirakova et al., 2006), similar to several hypothetical Mycobacterium tuberculosis strain H37Rv proteins e.g. O06795|YH60_MYCTU|Rv1760|MTCY28.26 hypothetical 54.1 KDA protein (502 aa), FASTA scores: opt: 586, E(): 9.8e-29,(28.95% identity in 463 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008).; Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1 3496366 tgs1 888841 tgs1 Mycobacterium tuberculosis H37Rv Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1 NP_217646.1 3494975 R 83332 CDS NP_217647.3 448824799 888838 3496551..3497549 1 NC_000962.3 Rv3131, (MTCY03A2.27c), len: 332 aa. Conserved protein, similar to other hypothetical bacterial proteins e.g. O53476|Rv2032|MTV018.19 (331 aa), FASTA scores: opt: 568, E(): 2.5e-27, (36.7% identity in 321 aa overlap); O05800|Rv3127|MTCY164.37 (344 aa), FASTA scores: opt: 521,E(): 1.9e-24, (34.95% identity in 326 aa overlap); Q9RI33|SCJ12.27c from Streptomyces coelicolor (335 aa),FASTA scores: opt: 441, E(): 1.3e-19, (35.75% identity in 319 aa overlap); Q9RI44|SCJ12.14 from Streptomyces coelicolor (309 aa), FASTA scores: opt: 328, E(): 9.3e-13,(27.9% identity in 308 aa overlap); Q9CBP5|ML1751 from Mycobacterium leprae (721 aa), FASTA scores: opt: 137, E(): 0.78, (26.15% identity in 298 aa overlap); etc. Equivalent to AAK47555 from Mycobacterium tuberculosis strain CDC1551 but shorter 12 aa. Predicted possible vaccine candidate (See Zvi et al., 2008).; hypothetical protein 3497549 888838 Rv3131 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217647.3 3496551 D 83332 CDS NP_217648.1 15610268 888829 complement(3497529..3499265) 1 NC_000962.3 Rv3132c, (MTCY03A2.26), len: 578 aa. DevS (alternate gene name: dosS), membrane-bound two component sensor histidine kinase (see citations below; dev for Differentially Expressed in Virulent strain), similar to others two component sensors e.g. Q9RI43|SCJ12.15c putative two-component sensor from Streptomyces coelicolor (585 aa),FASTA scores: opt: 1305, E(): 2.5e-69, (41.35% identity in 573 aa overlap); Q9ZBY4|SCD78.15 putative two component sensor from Streptomyces coelicolor (560 aa), FASTA scores: opt: 1194, E(): 8.1e-63, (41.05% identity in 558 aa overlap); O85371|CPRS two component regulator from Rhodococcus sp (563 aa), FASTA scores: opt: 803, E(): 8.3e-40, (38.4% identity in 552 aa overlap); Q9L094|SCC24.23 putative two-component sensor histidine kinase from Streptomyces coelicolor (similarity only in C-terminus for this one); etc. Also highly similar to mycobacterium O53473|Rv2027c|MTV018.14c putative membrane protein (573 aa), FASTA scores: opt: 2333, E(): 7.6e-130,(61.45% identity in 576 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). Contains GAF domain that binds heme.; Two component sensor histidine kinase DevS 3499265 devS 888829 devS Mycobacterium tuberculosis H37Rv Two component sensor histidine kinase DevS NP_217648.1 3497529 R 83332 CDS NP_217649.1 15610269 888842 complement(3499262..3499915) 1 NC_000962.3 Rv3133c, (MTCY03A2.25), len: 217 aa. DevR (alternate gene name: dosR), two component transcriptional regulator (see Dasgupta et al., 2000; dev for Differentially Expressed in Virulent strain), highly similar to several e.g. O85372|CPRR two component regulator from Rhodococcus sp. (212 aa), FASTA scores: opt: 868, E(): 6.2e-46, (65.05% identity in 206 aa overlap); Q9RI42|SCJ12.16c putative LuxR family two-component response regulator from Streptomyces coelicolor (233 aa), FASTA scores: opt: 849, E(): 9.7e-45,(60.55% identity in 218 aa overlap); Q9XA59|SCGD3.19 putative two-component system response transcriptional regulator from Streptomyces coelicolor (218 aa), FASTA scores: opt: 835, E(): 6.5e-44, (61.55% identity in 208 aa overlap); and similar to others. Contains bacterial regulatory proteins, LuxR family signature (PS00622) near C-terminus as seen in bvgA, comA, dctR, degU, evgA, fimZ,fixJ, gacA, glpR, narL, narP, nodW, rcsB and uhpA. Helix-turn-helix motif at 166-187 (+3.15 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. The N-terminal region is similar to that of other regulatory components of sensory transduction systems.; Two component transcriptional regulatory protein DevR (probably LuxR/UhpA-family) 3499915 devR 888842 devR Mycobacterium tuberculosis H37Rv Two component transcriptional regulatory protein DevR (probably LuxR/UhpA-family) NP_217649.1 3499262 R 83332 CDS NP_217650.1 15610270 887558 complement(3499943..3500749) 1 NC_000962.3 Rv3134c, (MTCY03A2.240, len: 268 aa. Universal stress protein family protein. Ala-, Val- rich (see citations below), related to other hypothetical Mycobacterium tuberculosis proteins e.g. O53474|Rv2028c|MTV018.15c (279 aa), FASTA scores: opt: 562,E(): 3.2e-28, (40.65% identity in 273 aa overlap); O06188|Rv2624c|MTCY01A10.08 (272 aa), FASTA scores: opt: 458, E(): 1.1e-21, (36.55% identity in 271 aa overlap); O53472|R2026c|MTV018.13c (294 aa), FASTA scores: opt: 232,E(): 1.9e-07, (30.45% identity in 276 aa overlap); etc. Shares some similarity with other hypothetical proteins from Streptomyces coelicolor e.g. Q9RIZ8|SCJ1.16c (294 aa),FASTA scores: opt: 207, E(): 6.9e-06, (28.9% identity in 263 aa overlap); Q9K4L5|SC5F8.09 putative stress-inducible protein (312 aa), FASTA scores: opt: 204, E(): 1.1e-05,(28.4% identity in 271 aa overlap); etc. Equivalent to AAK47558|MT3220 Universal stress protein family from Mycobacterium tuberculosis strain CDC1551 (268 aa). Rv3134c seems cotranscribed with devR-devS (see Sherman et al.,2001).; Universal stress protein family protein 3500749 887558 Rv3134c Mycobacterium tuberculosis H37Rv Universal stress protein family protein NP_217650.1 3499943 R 83332 CDS YP_177934.1 57117064 888153 3501334..3501732 1 NC_000962.3 Rv3135, (MTCY03A2.23c), len: 132 aa. PPE50, Member of the Mycobacterium tuberculosis Ala-, Gly-rich PPE family, similar to P95190|Rv3136|MTCY03A2.22c (380 aa),FASTA scores: opt: 494, E(): 6.7e-25, (57.25% identity in 131 aa overlap) (next ORF downstream), MTY21C12_9,MTCY3C7_24, MTCI125_27, MTV049_12, MTV049_9, MTV049_11,MTCY274_24 etc.; PPE family protein PPE50 3501732 PPE50 888153 PPE50 Mycobacterium tuberculosis H37Rv PPE family protein PPE50 YP_177934.1 3501334 D 83332 CDS YP_177935.1 57117065 888835 3501794..3502936 1 NC_000962.3 Rv3136, (MTCY03A2.22c), len: 380 aa. PPE51, Member of the Mycobacterium tuberculosis Ala-, Gly-rich PPE family, similar to Q9AGF0|Ov2770c Rv2770c-like protein from M. microti (397 aa), FASTA scores: opt: 917, E(): 9e-41,(46.15% identity in 388 aa overlap); O33312|Rv2770c|MTV002.35c, MTV002_36, MTCI125_26,MTCY10G2_10, MTCI364_8, MTV049_28, MTV049_29, etc.; PPE family protein PPE51 3502936 PPE51 888835 PPE51 Mycobacterium tuberculosis H37Rv PPE family protein PPE51 YP_177935.1 3501794 D 83332 CDS YP_007411850.1 448817977 14515898 complement(3502945..3503277) 1 NC_000962.3 Rv3136A, len: 110 aa. Conserved protein.; hypothetical protein 3503277 14515898 Rv3136A Mycobacterium tuberculosis H37Rv hypothetical protein YP_007411850.1 3502945 R 83332 CDS NP_217653.1 15610273 888827 3503393..3504175 1 NC_000962.3 Rv3137, (MTCY03A2.21c), len: 260 aa. Probable monophosphatase, equivalent to O32889|MLCB1779_19|ML0662 putative monophosphatase from Mycobacterium leprae (255 aa), FASTA scores: opt: 1403, E(): 1.2e-81, (81.8% identity in 253 aa overlap). Also similar to Q9K4B1|SC7E4.05c from Streptomyces coelicolor (266 aa), FASTA scores: opt: 969,E(): 3.5e-54, (57.9% identity in 259 aa overlap); Q53743|PUR3 mono-phosphatase from Streptomyces lipmanii (Streptomyces alboniger) (273 aa), FASTA scores: opt: 862,E(): 2.1e-47, (55.25% identity in 257 aa overlap); BAB50023|MLL3039 mono-phosphatase from Rhizobium loti (Mesorhizobium loti) (262 aa), FASTA scores: opt: 448, E(): 3.2e-21, (31.37% identity in 255 aa overlap); etc. Contains inositol monophosphatase family signature 1 (PS00629).; Probable monophosphatase 3504175 888827 Rv3137 Mycobacterium tuberculosis H37Rv Probable monophosphatase NP_217653.1 3503393 D 83332 CDS NP_217654.1 15610274 887973 3504195..3505283 1 NC_000962.3 Rv3138, (MTCY03A2.20c), len: 362 aa. Probable pflA,pyruvate formate lyase activating protein, similar to other e.g. Q9V0N1|PAB1859 from Pyrococcus abyssi (348 aa), FASTA scores: opt: 926, E(): 1.1e-52, (39.95% identity in 343 aa overlap); O27446|MTH1395 from Methanobacterium thermoautotrophicum (335 aa), FASTA scores: opt: 909, E(): 1.3e-51, (42.2% identity in 327 aa overlap); O28939|AF1330 from Archaeoglobus fulgidus (336 aa), FASTA scores: opt: 884, E(): 5.6e-50, (42.0% identity in 319 aa overlap); etc. Also similar to O50099|PH1391 hypothetical 40.2 KDA protein from Pyrococcus horikoshii (348 aa), FASTA scores: opt: 934, E(): 3.3e-53, (40.5% identity in 343 aa overlap); and other hypothetical proteins.; Probable pyruvate formate lyase activating protein PflA (formate acetyltransferase activating enzyme) ([pyruvate formate-lyase] activating enzyme) 3505283 pflA 887973 pflA Mycobacterium tuberculosis H37Rv Probable pyruvate formate lyase activating protein PflA (formate acetyltransferase activating enzyme) ([pyruvate formate-lyase] activating enzyme) NP_217654.1 3504195 D 83332 CDS NP_217655.1 15610275 887971 3505363..3506769 1 NC_000962.3 Rv3139, (MTCY03A2.19c), len: 468 aa. Probable fadE24, acyl-CoA dehydrogenase (1.3.99.-), equivalent to O32890|MLCB1779.30|FADE24|ML0661 putative acyl-CoA dehydrogenase from Mycobacterium leprae (465 aa), FASTA scores: opt: 2587, E(): 4e-153, (83.6% identity in 464 aa overlap). Similar to other e.g. Q9HUH0|PA4995 from Pseudomonas aeruginosa (429 aa), FASTA scores: opt: 1139,E(): 2.8e-63, (45.3% identity in 426 aa overlap); Q9K6D0|MMGC|BH3799 from Bacillus halodurans (379 aa), FASTA scores: opt: 603, E(): 4.7e-30, (30.3% identity in 366 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 601, E(): 6.3e-30, (32.25% identity in 363 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073) near C-terminus. Belongs to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE24 3506769 fadE24 887971 fadE24 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE24 NP_217655.1 3505363 D 83332 CDS NP_217656.1 15610276 887417 3506790..3507995 1 NC_000962.3 Rv3140, (MTCY03A2.18c), len: 401 aa. Probable fadE23, acyl-CoA dehydrogenase (1.3.99.-) (see citation below), equivalent to O32891|MLCB1779.31|FADE23|ML0660 putative acyl-CoA dehydrogenase from Mycobacterium leprae (400 aa), FASTA scores: opt: 2307, E(): 3e-136, (89.5% identity in 401 aa overlap). Also similar to others e.g. Q9HUH1|PA4994 from Pseudomonas aeruginosa (402 aa), FASTA scores: opt: 1558, E(): 1.2e-89, (61.0% identity in 400 aa overlap); O31251 from Acinetobacter sp. ADP1 (401 aa),FASTA scores: opt: 1509, E(): 1.3e-86, (58.2% identity in 402 aa overlap); Q9K6D1|ACDA or BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 612, E(): 8.4e-31,(38.2% identity in 293 aa overlap); Q9AHX9|FADFX from Pseudomonas putida (375 aa), FASTA scores: opt: 584, E(): 4.6e-29, (32.7% identity in 379 aa overlap); etc. Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE23 3507995 fadE23 887417 fadE23 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE23 NP_217656.1 3506790 D 83332 CDS NP_217657.1 15610277 888051 3508095..3509066 1 NC_000962.3 Rv3141, (MTCY03A2.17c), len: 323 aa. Probable fadB4,quinone oxidoreductase, showing strong similarity to variety of quinone oxidoreductases and domains in polyketide and fatty acid synthases e.g. Q9HTV6|PA5234 probable oxidoreductase from Pseudomonas aeruginosa (325 aa), FASTA scores: opt: 737, E(): 1.4e-35, (39.65% identity in 328 aa overlap); Q9RYQ7|DRA0251 putative NADPH quinone oxidoreductase from Deinococcus radiodurans (336 aa), FASTA scores: opt: 688, E(): 1e-32, (40.6% identity in 325 aa overlap); Q9RVG8|DR1061 putative NADPH quinone oxidoreductase from Deinococcus radiodurans (388 aa), FASTA scores: opt: 559, E(): 3.3e-25, (36.3% identity in 325 aa overlap); BAB49685|MLL2594 probable quinone oxidoreductase from Rhizobium loti (Mesorhizobium loti) (326 aa), FASTA scores: opt: 519, E(): 5.9e-23, (34.25% identity in 330 aa overlap); Q9LXZ4|T5P19_110 quinone reductase-like protein from Arabidopsis thaliana (348 aa), FASTA scores: opt: 517,E(): 8.1e-23, (33.55% identity in 322 aa overlap); etc. Also similar to Q9AA38|CC0770 zinc-containing alcohol dehydrogenase from Caulobacter crescentus (325 aa), FASTA scores: opt: 673, E(): 7.2e-32, (40.2% identity in 326 aa overlap); and Q9ABX4|CC0096 zinc-containing alcohol dehydrogenase from Caulobacter crescentus (332 aa), FASTA scores: opt: 623, E(): 5.7e-29, (40.7% identity in 334 aa overlap). Also resembles Mycobacterium tuberculosis proteins P96826|Rv0149|MTCI5_23, MTCY13D12.11, MTCY24G1.03,MTCY19H9.01. Belongs to the zinc-containing alcohol dehydrogenase family, quinone oxidoreductase subfamily. Thought to be differentially expressed within host cells (see Triccas et al., 1999).; Probable NADPH quinone oxidoreductase FadB4 (NADPH:quinone reductase) (zeta-crystallin) 3509066 fadB4 888051 fadB4 Mycobacterium tuberculosis H37Rv Probable NADPH quinone oxidoreductase FadB4 (NADPH:quinone reductase) (zeta-crystallin) NP_217657.1 3508095 D 83332 CDS NP_217658.1 15610278 887521 complement(3509118..3509546) 1 NC_000962.3 Rv3142c, (MTCY03A2.16), len: 142 aa. Hypothetical unknown protein. Equivalent to AAK47569 from Mycobacterium tuberculosis strain CDC1551 but shorter 33 aa.; Hypothetical protein 3509546 887521 Rv3142c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217658.1 3509118 R 83332 CDS NP_217659.1 15610279 887576 3509654..3510055 1 NC_000962.3 Rv3143, (MTCY03A2.15c), len: 133 aa. Probable response regulator, similar to other sensory transduction regulatory proteins e.g. Q9X810|SC6G10.25 from Streptomyces coelicolor (133 aa), FASTA scores: opt: 474, E(): 2.8e-24,(54.15% identity in 120 aa overlap); Q9KZ82|SCE25.04c from Streptomyces coelicolor (225 aa), FASTA scores: opt: 144,E(): 0.016, (32.3% identity in 127 aa overlap); Q9RZT4|DRB0029 from Deinococcus radiodurans (416 aa), FASTA scores: opt: 145, E(): 0.024, (30.65% identity in 124 aa overlap). Similar to other regulatory components of sensory transduction systems.; Probable response regulator 3510055 887576 Rv3143 Mycobacterium tuberculosis H37Rv Probable response regulator NP_217659.1 3509654 D 83332 CDS YP_177936.2 448824800 887930 complement(3510088..3511317) 1 NC_000962.3 Rv3144c, (MTCY03A2.14), len: 409 aa. PPE52, Member of the Mycobacterium tuberculosis PPE family, Gly-,Ala-rich, similar to others e.g. P71868|Rv3533c|MTCY03C7.23 (582 aa), FASTA scores: opt: 1007, E(): 5.2e-35, (56.2% identity in 306 aa overlap); and MTV014_3, MTCY6G11_5,MTCY98.0034c, MTCY31.06c, MTCY48.17, MTCY98.0029c,MTCY03C7.17c, etc. Nucleotide position 3510642 in the genome sequence has been corrected, T:C resulting in S226G.; PPE family protein PPE52 3511317 PPE52 887930 PPE52 Mycobacterium tuberculosis H37Rv PPE family protein PPE52 YP_177936.2 3510088 R 83332 CDS NP_217661.1 15610281 887397 3511682..3512068 1 NC_000962.3 Rv3145, (MTCY03A2.13c), len: 128 aa. Probable nuoA,integral membrane NADH dehydrogenase, chain A, similar to others e.g. Q9XAQ4|NUOA from Streptomyces coelicolor (119 aa), FASTA scores: opt: 405, E(): 5.4e-20, (68.75% identity in 128 aa overlap); Q9RU86|DR1506 from Deinococcus radiodurans (160 aa), FASTA scores: opt: 327, E(): 9e-15,(40.3% identity in 124 aa overlap); BAB47039|NDHC from Triticum aestivum (Wheat), FASTA scores: opt: 273, E(): 2.6e-11, (38.1% identity in 126 aa overlap); etc. Also similar to a NADH-plastoquinone oxidoreductases e.g. P26303|NU3C_WHEAT|NDHC from Triticum aestivum (Wheat) (120 aa), FASTA scores: opt: 273, E(): 2.6e-1, (38.1% identity in 126 aa overlap). Belongs to the complex I subunit 3 family.; Probable NADH dehydrogenase I (chain A) NuoA (NADH-ubiquinone oxidoreductase chain A) 3512068 nuoA 887397 nuoA Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain A) NuoA (NADH-ubiquinone oxidoreductase chain A) NP_217661.1 3511682 D 83332 CDS NP_217662.1 15610282 888791 3512077..3512631 1 NC_000962.3 Rv3146, (MTCY03A2.12c), len: 184 aa. Probable nuoB,NADH dehydrogenase, chain B, similar to others e.g. Q9XAQ5|NUOB from Streptomyces coelicolor (184 aa), FASTA scores: opt: 989, E(): 1.4e-56, (78.25% identity in 184 aa overlap); Q56218|NQO6_THETH|NQO6 from Thermus aquaticus (subsp. thermophilus) (181 aa), FASTA scores: opt: 720,E(): 2.6e-39, (64.45% identity in 152 aa overlap); Q9RU87|DR1505 from Deinococcus radiodurans (181 aa), FASTA scores: opt: 719, E(): 3e-39, (62.6% identity in 155 aa overlap); etc. Belongs to the complex I 20 KDA subunit family. May contain an iron-sulfur 4FE-4S cluster.; Probable NADH dehydrogenase I (chain B) NuoB (NADH-ubiquinone oxidoreductase chain B) 3512631 nuoB 888791 nuoB Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain B) NuoB (NADH-ubiquinone oxidoreductase chain B) NP_217662.1 3512077 D 83332 CDS NP_217663.1 15610283 888816 3512628..3513338 1 NC_000962.3 Rv3147, (MTCY03A2.11c), len: 236 aa. Probable nuoC,NADH dehydrogenase, chain C, similar to others e.g. Q9XAQ6|NUOC from Streptomyces coelicolor (251 aa), FASTA scores: opt: 1113, E(): 2.6e-64, (67.35% identity in 236 aa overlap); Q9A6X2|CC1954 from Caulobacter crescentus (197 aa), FASTA scores: opt: 351, E(): 1.6e-15, (41.65% identity in 132 aa overlap); BAB48757|MLL1369 from Rhizobium loti (Mesorhizobium loti) (201 aa), FASTA scores: opt: 347, E(): 3e-15, (42.4% identity in 132 aa overlap); etc. Also similar to Q9UUU0|NUGM NUGM protein precursor from Yarrowia lipolytica (Candida lipolytica) (281 aa), FASTA scores: opt: 356, E(): 1.1e-15, (34.55% identity in 162 aa overlap). Also similar to MTCY251.05, FASTA score: E():4.9e-05. Equivalent to AAK47574 from Mycobacterium tuberculosis strain CDC1551 but longer 26 aa. Belongs to the complex I 30 KDA subunit family.; Probable NADH dehydrogenase I (chain C) NuoC (NADH-ubiquinone oxidoreductase chain C) 3513338 nuoC 888816 nuoC Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain C) NuoC (NADH-ubiquinone oxidoreductase chain C) NP_217663.1 3512628 D 83332 CDS NP_217664.1 15610284 888851 3513338..3514660 1 NC_000962.3 Rv3148, (MTCY03A2.10c), len: 440 aa. Probable nuoD,NADH dehydrogenase, chain B, similar to others e.g. Q9XAQ7|NUOD from Streptomyces coelicolor (440 aa), FASTA scores: opt: 2198, E(): 1e-131, (73.9% identity in 429 aa overlap); P15689|NUCM_PARTE from Paramecium tetraurelia (400 aa), FASTA scores: opt: 922, E(): 5.8e-51, (38.5% identity in 408 aa overlap); Q9RU89|NUOD_DEIRA|DR1503 from Deinococcus radiodurans (401 aa), FASTA scores: opt: 922,E(): 5.8e-51, (47.75% identity in 404 aa overlap); etc. Equivalent to AAK47575 from Mycobacterium tuberculosis strain CDC1551 but longer 42 aa. Contains helix-turn-helix motif at aa 340-361. Belongs to the complex I 49 KDA subunit family.; Probable NADH dehydrogenase I (chain D) NuoD (NADH-ubiquinone oxidoreductase chain D) 3514660 nuoD 888851 nuoD Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain D) NuoD (NADH-ubiquinone oxidoreductase chain D) NP_217664.1 3513338 D 83332 CDS NP_217665.1 15610285 887903 3514657..3515415 1 NC_000962.3 Rv3149, (MTCY03A2.09c), len: 252 aa. Probable nuoE,NADH dehydrogenase, chain E, similar to others e.g. Q9XAQ8|NUOE from Streptomyces coelicolor (290 aa), FASTA scores: opt: 1002, E(): 5.7e-55, (69.5% identity in 213 aa overlap); P40915|NUHM_NEUCR|NUO-24 from Neurospora crassa (263 aa), FASTA scores: opt: 412, E(): 1.9e-18, (38055% identity in 192 aa overlap); P19234|NUHM_RAT from Rattus norvegicus (Rat) (241 aa), FASTA scores: opt: 410, E(): 2.4e-18, (23.9% identity in 237 aa overlap); etc. Belongs to the complex I 24 KDA subunit family. Binds a 2FE-2S cluster (potential).; Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E) 3515415 nuoE 887903 nuoE Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E) NP_217665.1 3514657 D 83332 CDS NP_217666.1 15610286 888854 3515412..3516749 1 NC_000962.3 Rv3150, (MTCY03A2.08c), len: 445 aa. Probable nuoF,NADH dehydrogenase, chain F, similar to others e.g. Q9XAQ9|NUOF_STRCO from Streptomyces coelicolor (449 aa),FASTA scores: opt: 2314, E(): 3.5e-139, (76.25% identity in 434 aa overlap); NUF2_RHIME from Rhizobium meliloti (421 aa), FASTA scores: opt: 1545, E(): 1.8e-90, (53.1% identity in 424 aa overlap); Q9RU92|DR1500 from Deinococcus radiodurans (444 aa), FASTA scores: opt: 1445, E(): 4.1e-84, (52.9% identity in 427 aa overlap); etc. Contains respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2 (PS00645). Belongs to the complex I 51 KDA subunit family. Cofactor: FMN and one 4FE-4S cluster (probable).; Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F) 3516749 nuoF 888854 nuoF Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F) NP_217666.1 3515412 D 83332 CDS NP_217667.1 15610287 887540 3516746..3519166 1 NC_000962.3 Rv3151, (MTCY03A2.07c), len: 806 aa. Probable nuoG,NADH dehydrogenase I, chain G, similar to others e.g. Q9XAR0|NUOG_STRCO from Streptomyces coelicolor (843 aa),FASTA scores: opt: 1968 ,E(): 5.2e-107, (62.45% identity in 818 aa overlap); P56914|NUG2_RHIME from Rhizobium meliloti (853 aa), FASTA scores: opt: 964, E(): 1.6e-48, (30.6% identity in 840 aa overlap); etc. But also similarity with other proteins e.g. P77908|FDHA formate dehydrogenase,alpha subunit (formate dehydrogenase [NADP+]) from Moorella thermoacetica (Clostridium thermoaceticum) (893 aa), FASTA scores: opt: 928, E(): 2e-46, (28.65% identity in 865 aa overlap); and Q9UUU3|NUAM NUAM protein precursor from Yarrowia lipolytica (Candida lipolytica) (728 aa), FASTA scores: opt: 894, E(): 1.7e-44, (31.95% identity in 676 aa overlap). Equivalent to AAK47578 from Mycobacterium tuberculosis strain CDC1551 but longer 15 aa. Contains respiratory-chain NADH dehydrogenase 75 kDa subunit signature 2 (PS00642). Belongs to the complex I 75 KDA subunit family. Cofactor: may bind two 4FE-4S cluster and one 2FE-2S cluster.; Probable NADH dehydrogenase I (chain G) NuoG (NADH-ubiquinone oxidoreductase chain G) 3519166 nuoG 887540 nuoG Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain G) NuoG (NADH-ubiquinone oxidoreductase chain G) NP_217667.1 3516746 D 83332 CDS NP_217668.1 15610288 887531 3519282..3520514 1 NC_000962.3 Rv3152, (MTCY03A2.06c), len: 410 aa. Probable nuoH,integral membrane NADH dehydrogenase I, chain H, similar to others e.g. Q9XAR1 Q9XAR1|NUOH from Streptomyces coelicolor (467 aa), FASTA scores: opt: 1630, E(): 3.4e-90, (58.35% identity in 413 aa overlap); Q9RU94|DR1498 from Deinococcus radiodurans (397 aa), FASTA scores: opt: 1081, E(): 2e-57,(45.5% identity in 391 aa overlap); Q9ZCF7|NUOH_RICPR|RP796 from Rickettsia prowazekii (339 aa), FASTA scores: opt: 976, E(): 3.4e-51, (46.2% identity in 329 aa overlap); etc. Contains respiratory-chain NADH dehydrogenase subunit 1 signature 2 (PS00668). Some similarity to MTCY251.02 (FASTA score: E(): 1.2e-07). Belongs to the complex I subunit 1 family.; Probable NADH dehydrogenase I (chain H) NuoH (NADH-ubiquinone oxidoreductase chain H) 3520514 nuoH 887531 nuoH Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain H) NuoH (NADH-ubiquinone oxidoreductase chain H) NP_217668.1 3519282 D 83332 CDS NP_217669.1 15610289 887530 3520507..3521142 1 NC_000962.3 Rv3153, (MTCY03A2.05c), len: 211 aa. Probable nuoI,NADH dehydrogenase I, chain I, similar to others e.g. Q9XAR2|NUOI from Streptomyces coelicolor (211 aa), FASTA scores: opt: 825, E(): 9.3e-44, (70.1% identity in 164 aa overlap); Q56224|NQO9_THETH from Thermus aquaticus (subsp. thermophilus) (182 aa), FASTA scores: opt: 543, E(): 1.8e-26, (50.9% identity in 163 aa overlap); Q9RU95|DR1497 from Deinococcus radiodurans (178 aa), FASTA scores: opt: 527, E(): 1.7e-25, (48.75% identity in 162 aa overlap); etc. Contains two 4Fe-4S ferredoxins, iron-sulfur binding region signatures (PS00198). Belongs to the complex I 23 KDA subunit family. The iron-sulfur centers are similar to those of 'bacterial-type' 4FE-4S ferredoxins. Cofactor: binds two 4FE-4S clusters.; Probable NADH dehydrogenase I (chain I) NuoI (NADH-ubiquinone oxidoreductase chain I) 3521142 nuoI 887530 nuoI Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain I) NuoI (NADH-ubiquinone oxidoreductase chain I) NP_217669.1 3520507 D 83332 CDS NP_217670.1 15610290 888762 3521139..3521927 1 NC_000962.3 Rv3154, (MTCY03A2.04c), len: 262 aa. Probable nuoJ,transmembrane NADH dehydrogenase I, chain J, similar to others e.g. Q9XAR3|NUOJ from Streptomyces coelicolor (285 aa), FASTA scores: opt: 991, E(): 3.2e-52, (63.7% identity in 243 aa overlap); Q9JX90|NUOJ|NMA0006 from Neisseria meningitidis (serogroup A) (223 aa), FASTA scores: opt: 329, E(): 9.6e-13, (34.85% identity in 175 aa overlap); Q9K1B2|NMB0253 from Neisseria meningitidis (serogroup B) (223 aa), FASTA scores: opt: 326, E(): 1.5e-12, (34.85% identity in 175 aa overlap); etc. But also similarity with Q00243|NU6C_PLEBO|NDH6 NADH-plastoquinone oxidoreductase chain 6 homolog (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) from Plectonema boryanum (199 aa),FASTA scores: opt: 287, E(): 2.8e-10, (34.35% identity in 195 aa overlap). Similar to polypeptide 6 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondria.; Probable NADH dehydrogenase I (chain J) NuoJ (NADH-ubiquinone oxidoreductase chain J) 3521927 nuoJ 888762 nuoJ Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain J) NuoJ (NADH-ubiquinone oxidoreductase chain J) NP_217670.1 3521139 D 83332 CDS NP_217671.1 15610291 888764 3521924..3522223 1 NC_000962.3 Rv3155, (MTCY03A2.03c), len: 99 aa. Probable nuoK,integral membrane NADH dehydrogenase I, chain K, similar to others e.g. Q9XAR4|NUOK from Streptomyces coelicolor (99 aa), FASTA scores: opt: 509, E(): 2.7e-31, (78.55% identity in 98 aa overlap); Q56226|NQOB_THETH|NQO11 from Thermus aquaticus (subsp. thermophilus) (95 aa), blast scores: initn: 298, init1: 180, bits: 85.7, FASTA scores: opt: 313,E(): 9.4e-17, (53.7% identity in 95 aa overlap); Q9RU97|DR1495 from Deinococcus radiodurans (103 aa), FASTA scores: opt: 309, E(): 2e-16, (52.0% identity in 100 aa overlap); etc. But also similarity with NADH-plastoquinone oxidoreductases chain 4L e.g. Q9MUL4|NULC_MESVI|NDHE from Mesostigma viride (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) (101 aa), FASTA scores: opt: 280,E(): 2.8e-14, (40.6% identity in 101 aa overlap); and P06261|NULC_TOBAC|NDHE|NDH4L from Nicotiana tabacum (Common tobacco) (101 aa), FASTA scores: opt: 259, E(): 1e-12,(43.0% identity in 93 aa overlap). Similar to polypeptide 4L of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondria.; Probable NADH dehydrogenase I (chain K) NuoK (NADH-ubiquinone oxidoreductase chain K) 3522223 nuoK 888764 nuoK Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain K) NuoK (NADH-ubiquinone oxidoreductase chain K) NP_217671.1 3521924 D 83332 CDS NP_217672.1 15610292 888063 3522234..3524135 1 NC_000962.3 Rv3156, (MTCY03A2.02c), len: 633 aa. Probable nuoL,integral membrane NADH dehydrogenase I, chain L, similar to others e.g. Q9XAR5|NUOL_STRCO from Streptomyces coelicolor (654 aa), FASTA scores: opt: 2074, E(): 1.1e-111, (61.1% identity in 648 aa overlap); Q56227|NQOC_THETH|NQO12 from Thermus aquaticus (subsp. thermophilus) (606 aa), FASTA scores: opt: 1420, E(): 3.8e-74, (43.35% identity in 630 aa overlap); Q9ZJV6|NUOL|JHP1192 from Helicobacter pylori J99 (Campylobacter pylori J99) (612 aa), FASTA scores: opt: 1279, E(): 4.7e-66, (41.65% identity in 516 aa overlap); etc. Also similar to MTCY251.04 (FASTA score: E(): 1.3e-11) and MTCY03A2.01c (FASTA score: E(): 2.3e-10). Similar to polypeptide 5 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondrial.; Probable NADH dehydrogenase I (chain L) NuoL (NADH-ubiquinone oxidoreductase chain L) 3524135 nuoL 888063 nuoL Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain L) NuoL (NADH-ubiquinone oxidoreductase chain L) NP_217672.1 3522234 D 83332 CDS NP_217673.1 15610293 888765 3524132..3525793 1 NC_000962.3 Rv3157, (MTCY03A2.01c-MTV014.01c), len: 553 aa. Probable nuoM, integral membrane NADH dehydrogenase I,chain M, similar to others e.g. Q9XAR6|NUOM from Streptomyces coelicolor (523 aa), FASTA scores: opt: 1621,E(): 4.2e-89, (56.55% identity in 541 aa overlap); P50974|NUOM_RHOCA|NUOM from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (512 aa), FASTA scores: opt: 996, E(): 6.5e-52, (38.2% identity in 521 aa overlap); P29925|NQOD_PARDE|NQO13 from Paracoccus denitrificans (513 aa), FASTA scores: opt: 987, E(): 2.2e-51, (37.05% identity in 540 aa overlap); etc. Also similar to MTCY251.04 (FASTA score: E(): 3.3e-16) and MTCY03A2.02c (FASTA score: E(): 9.6e-13). Similar to polypeptide 4 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondrial.; Probable NADH dehydrogenase I (chain M) NUOK (NADH-ubiquinone oxidoreductase chain M) 3525793 nuoM 888765 nuoM Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain M) NUOK (NADH-ubiquinone oxidoreductase chain M) NP_217673.1 3524132 D 83332 CDS NP_217674.1 15610294 888780 3525790..3527385 1 NC_000962.3 Rv3158, (MTV014.02c), len: 531 aa. Probable nuoN,integral membrane NADH dehydrogenase I, chain N, similar to others e.g. Q9XAR7|SC10A7.08c from Streptomyces coelicolor (552 aa), FASTA scores: opt: 1493, E(): 1.1e-81, (56.7% identity in 543 aa overlap); Q9PGI2|XF0318 from Xylella fastidiosa (485 aa), FASTA scores: opt: 942, E(): 7.4e-49,(39.6% identity in 379 aa overlap); CAB51628|NUON2 from Rhizobium meliloti (Sinorhizobium meliloti) (479 aa), FASTA scores: opt: 934, E(): 2.2e-48, (35.5% identity in 479 aa overlap); etc. But also similarity with NADH-plastoquinone oxidoreductases chain 4L (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) e.g. P29801|NU2C_SYNP7|NDHB from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (521 aa), FASTA scores: opt: 921, E(): 1.4e-47, (40.25% identity in 395 aa overlap). Belongs to the complex I subunit 2 family.; Probable NADH dehydrogenase I (chain N) NuoN (NADH-ubiquinone oxidoreductase chain N) 3527385 nuoN 888780 nuoN Mycobacterium tuberculosis H37Rv Probable NADH dehydrogenase I (chain N) NuoN (NADH-ubiquinone oxidoreductase chain N) NP_217674.1 3525790 D 83332 CDS YP_177937.1 57117067 888794 complement(3527391..3529163) 1 NC_000962.3 Rv3159c, (MTV014.03c), len: 590 aa. PPE53, Member of the Mycobacterium tuberculosis PPE_family of Gly-, Asn-rich proteins. Highly similar to P71868|Rv3533c|MTCY03C7.23 (582 aa), FASTA scores: opt: 2289, E(): 3.2e-98, (63.5% identity in 600 aa overlap); and also similar to MTCY48_17,MTV041_29, MTCY6G11_5, MTCY98_24, etc. Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE53 3529163 PPE53 888794 PPE53 Mycobacterium tuberculosis H37Rv PPE family protein PPE53 YP_177937.1 3527391 R 83332 CDS NP_217676.1 15610296 888797 complement(3529338..3529979) 1 NC_000962.3 Rv3160c, (MTV014.04c), len: 213 aa. Possible transcriptional regulator, with some similarity to others e.g. Q9S3L4|AMTR AMTR protein (global repressor in the nitrogen regulation system; see Jakoby et al., 2000) (222 aa), FASTA scores: opt: 182, E(): 7.3e-05, (27.9% identity in 208 aa overlap); Q9X7X9|SC6A5.33c putative regulatory protein from Streptomyces coelicolor (223 aa), FASTA scores: opt: 176, E(): 0.00018, (26.5% identity in 185 aa overlap); Q9XA31|SCH69.03c putative transcriptional regulator from Streptomyces coelicolor (209 aa), FASTA scores: opt: 173, E(): 0.00027, (27.25% identity in 176 aa overlap); BAB54133|MLL7734 transcriptional regulator from Rhizobium loti (Mesorhizobium loti) (213 aa), FASTA scores: opt: 172, E(): 0.00031, (23.55% identity in 204 aa overlap); etc. Also similar to hypothetical proteins from Mycobacterium tuberculosis strain H37Rv e.g. P96839|Rv3557v|MTCY06G11.04c (200 aa), FASTA scores: opt: 169, E(): 0.00046, (26.75% identity in 157 aa overlap). Contains probable helix-turn-helix motif from aa 31 to 52 (Score 1857, +5.51 SD). Similar to the TetR/AcrR family of transcriptional regulators.; Possible transcriptional regulatory protein (probably TetR-family) 3529979 888797 Rv3160c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably TetR-family) NP_217676.1 3529338 R 83332 CDS NP_217677.1 15610297 888800 complement(3529990..3531138) 1 NC_000962.3 Rv3161c, (MTV014.05c), len: 382 aa. Possible dioxygenase, similar to subunit of several dioxygenases and related proteins e.g. BAB50510|MLR3662 dioxygenase, alpha subunit from Rhizobium loti (Mesorhizobium loti) (400 aa),FASTA scores: opt: 413, E(): 6.2e-20, (28.4% identity in 331 aa overlap); Q9A3T0|CC3122 rieske 2FE-2S family protein from Caulobacter crescentus (404 aa), FASTA scores: opt: 405, E(): 2.1e-19, (27.95% identity in 372 aa overlap); Q9HTF4|PA5410 probable ring hydroxylating dioxygenase,alpha-subunit from Pseudomonas aeruginosa (429 aa), FASTA scores: opt: 392, E(): 1.6e-18, (25.8% identity in 399 aa overlap); Q9AGK6|PHTAA phthalate dioxygenase large subunit from Arthrobacter keyseri (473 aa), FASTA scores: opt: 385,E(): 5.2e-18, (34.0% identity in 206 aa overlap); P76253|YEAW_ECOLI putative dioxygenase, alpha subunit from Escherichia coli (374 aa), FASTA scores: opt: 376, E(): 1.7e-17, (27.05% identity in 344 aa overlap); etc.; Possible dioxygenase 3531138 888800 Rv3161c Mycobacterium tuberculosis H37Rv Possible dioxygenase NP_217677.1 3529990 R 83332 CDS NP_217678.1 15610298 888756 complement(3531208..3531645) 1 NC_000962.3 Rv3162c, (MTV014.06c), len: 145 aa. Possible integral membrane protein, with some similarity to C-terminal part of Q10803|Rv2877c|MTCY274.08c hypothetical protein from Mycobacterium tuberculosis (287 aa), FASTA scores: opt: 112, E(): 6.9, (29.65% identity in 135 aa overlap); and other hypothetical proteins from other organisms.; Possible integral membrane protein 3531645 888756 Rv3162c Mycobacterium tuberculosis H37Rv Possible integral membrane protein NP_217678.1 3531208 R 83332 CDS NP_217679.1 15610299 888789 complement(3531642..3532913) 1 NC_000962.3 Rv3163c, (MTV014.07c), len: 423 aa. Possible conserved secreted protein, with some similarity to other hypothetical bacterial proteins e.g. Q9Z539|SC9B2.20c from Streptomyces coelicolor (460 aa), FASTA scores: opt: 666,E(): 1.5e-33, (33.55% identity in 417 aa overlap); O58486|PH0774 from Pyrococcus horikoshii (410 aa), FASTA scores: opt: 329, E(): 6.9e-13, (23.8% identity in 424 aa overlap); Q9UZ66|PAB0849 from Pyrococcus abyssi (410 aa),FASTA scores: opt: 322, E(): 1.9e-12, (24.15% identity in 389 aa overlap); etc. Also some similarity with P71761|Rv1480|MTV007.27|MTCY277.01 from Mycobacterium tuberculosis (317 aa), FASTA scores: opt: 198, E(): 6.3e-05, (26.75% identity in 269 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature.; Possible conserved secreted protein 3532913 888789 Rv3163c Mycobacterium tuberculosis H37Rv Possible conserved secreted protein NP_217679.1 3531642 R 83332 CDS NP_217680.1 15610300 887658 complement(3532943..3533905) 1 NC_000962.3 Rv3164c, (MTV014.08c), len: 320 aa. Probable moxR3,methanol dehydrogenase regulatory protein, highly similar to Q9Z538|SC9B2.21c putative regulatory protein from Streptomyces coelicolor (332 aa), FASTA scores: opt: 1227,E(): 1.7e-67, (60.25% identity in 302 aa overlap); Q9UZ67|MOXR-3|PAB0848 methanol dehydrogenase regulatory protein from Pyrococcus abyssi (314 aa), FASTA scores: opt: 1126, E(): 2.3e-61, (54.1% identity in 305 aa overlap); Q9HSH7|MOXR|VNG0223G methanol dehydrogenase regulatory protein from Halobacterium sp. strain NRC-1 (318 aa), FASTA scores: opt: 1072, E(): 4.5e-58, (51.45% identity in 315 aa overlap); Q9RVV4|DR0918 MOXR-related protein from Deinococcus radiodurans (354 aa), FASTA scores: opt: 1000,E(): 1.2e-53, (50.95% identity in 318 aa overlap); etc. Also high similarity with several hypothetical bacterial proteins.; Probable methanol dehydrogenase transcriptional regulatory protein MoxR3 3533905 moxR3 887658 moxR3 Mycobacterium tuberculosis H37Rv Probable methanol dehydrogenase transcriptional regulatory protein MoxR3 NP_217680.1 3532943 R 83332 CDS NP_217681.1 15610301 887661 complement(3533913..3534395) 1 NC_000962.3 Rv3165c, (MTV014.09)c, len: 160 aa. Unknown protein.; hypothetical protein 3534395 887661 Rv3165c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217681.1 3533913 R 83332 CDS NP_217682.1 15610302 888750 complement(3534392..3535351) 1 NC_000962.3 Rv3166c, (MTV014.10c), len: 319 aa. Probable transmembrane protein, similar but longer (52 aa) to O32895|MLCB1779.35c hypothetical protein from Mycobacterium leprae (119 aa), FASTA scores: opt: 289, E(): 3.7e-10,(44.25% identity in 122 aa overlap). Also some similarity to Q9Z536|SC9B2.23c putative transmembrane protein from Streptomyces coelicolor (339 aa), FASTA scores: opt: 247,E(): 2.5e-07, (28.2% identity in 326 aa overlap); and in N-terminus to Q9RS20|DR2307 putative multidrug-efflux transporter from Deinococcus radiodurans (410 aa), FASTA scores: opt: 135,E(): 1, (32.35% identity in 136 aa overlap).; hypothetical protein 3535351 888750 Rv3166c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217682.1 3534392 R 83332 CDS NP_217683.1 15610303 888763 complement(3535431..3536057) 1 NC_000962.3 Rv3167c, (MTV014.11c), len: 208 aa. Probable transcriptional regulator, TetR family, similar to several transcriptional regulators e.g. Q9L2A4|SC8F4.22c (TetR/AcrR family) from Streptomyces coelicolor (234 aa), FASTA scores: opt: 317, E(): 7.5e-13, (33.35% identity in 210 aa overlap); Q9RK47|SCF12.11 (TetR/AcrR family) from Streptomyces coelicolor (206 aa), FASTA scores: opt: 293,E(): 2.1e-11, (32.65% identity in 199 aa overlap); Q54288 regulator of antibiotic transport complexes (TetR/AcrR family) (204 aa), FASTA scores: opt: 260, E(): 2.4e-09,(30.75% identity in 205 aa overlap); etc. Equivalent to AAK47595 from Mycobacterium tuberculosis strain CDC1551 but shorter 21 aa. Contains probable helix-turn-helix motif from aa 42 to 63 (Score 1727, +5.07 SD). May belong to the TetR/AcrR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably TetR-family) 3536057 888763 Rv3167c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR-family) NP_217683.1 3535431 R 83332 CDS NP_217684.1 15610304 888778 3536102..3537238 1 NC_000962.3 Rv3168, (MTV014.12), len: 378 aa. Putative aminoglycoside phosphotransferase, similar to hypothetical proteins e.g. Q9M7Y6|F3E22.6 from Arabidopsis thaliana (Mouse-ear cress) (314 aa), FASTA scores: opt: 236, E(): 1.1e-07, (27.35% identity in 234 aa overlap); Q9RYW2|DRA0194 from Deinococcus radiodurans (386 aa), FASTA scores: opt: 207, E(): 9.1e-06, (23.45% identity in 320 aa overlap); etc. Also some similarity with O69727|Rc3761c|MTV025.109c hypothetical protein from Mycobacterium tuberculosis (351 aa), FASTA scores: opt: 193, E(): 6.4e-05, (29.4% identity in 242 aa overlap).; Putative aminoglycoside phosphotransferase 3537238 888778 Rv3168 Mycobacterium tuberculosis H37Rv Putative aminoglycoside phosphotransferase NP_217684.1 3536102 D 83332 CDS NP_217685.1 15610305 888774 3537238..3538362 1 NC_000962.3 Rv3169, (MTV014.13), len: 374 aa. Conserved protein,with similarity to other hypothetical proteins: Q9A8W6|CC1232 from Caulobacter crescentus (368 aa), FASTA scores: opt: 669, E(): 3.3e-34, (34.05% identity in 376 aa overlap); and O32901|MLCB1779.41 from Mycobacterium leprae (127 aa), FASTA scores: opt: 179, E(): 0.00034, (29.0% identity in 131 aa overlap). Also weak similarity with P95149|Rv1866|MTCY359.07c (804 aa), FASTA scores: opt: 121,E(): 6.4, (37.0% identity in 119 aa overlap). Equivalent to AAK47597 from Mycobacterium tuberculosis strain CDC1551 but shorter 43 aa.; hypothetical protein 3538362 888774 Rv3169 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217685.1 3537238 D 83332 CDS NP_217686.1 15610306 888754 3538505..3539851 1 NC_000962.3 Rv3170, (MT3259, MTV014.14), len: 448 aa. Probable aofH, flavin-containing (mono)amine oxidase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to many eukaryotic monoamine oxidases e.g. P49253|AOF_ONCMY from Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (522 aa), FASTA scores: opt: 869, E(): 5.3e-44, (37.7% identity in 448 aa overlap); P21396|AOFA_RAT|MAOA from Rattus norvegicus (Rat) (526 aa), FASTA scores: opt: 839, E(): 3.2e-42, (37.45% identity in 446 aa overlap); Q99NA8|MAO-a from Cavia porcellus (Guinea pig) (506 aa), FASTA scores: opt: 836,E(): 4.6e-42, (37.0% identity in 446 aa overlap); P21398|AOFA_BOVIN from Bos taurus (Bovine) (527 aa), FASTA scores: opt: 806, E(): 2.8e-40, (37.0% identity in 446 aa overlap); P21397|AOFA_HUMAN (527 aa), FASTA scores: opt: 801, E(): 5.6e-40, (37.2% identity in 446 aa overlap); etc. Alternative start possible at position 3538487. Belongs to the flavin monoamine oxidase family. Cofactor: FAD (potential).; Probable flavin-containing monoamine oxidase AofH (amine oxidase) (MAO) 3539851 aofH 888754 aofH Mycobacterium tuberculosis H37Rv Probable flavin-containing monoamine oxidase AofH (amine oxidase) (MAO) NP_217686.1 3538505 D 83332 CDS NP_217687.1 15610307 887293 complement(3539846..3540745) 1 NC_000962.3 Rv3171c, (MTV014.15c), len: 299 aa. Possible hpx,non-heme haloperoxidase, similar to other hydrolases (principaly epoxide hydrolases) and non-heme chloroperoxidases e.g. Q9RKB6|SCE87.22c putative hydrolase from Streptomyces coelicolor (314 aa), FASTA scores: opt: 431, E(): 6e-20, (38.05% identity in 297 aa overlap); Q9HZ14|PA3226 probable hydrolase (similar to alpha/beta hydrolase fold) from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 236, E(): 1e-07, (29.6% identity in 277 aa overlap); Q9DBL9|1300003 D03RIK protein similar to alpha/beta hydrolase fold from Mus musculus (Mouse) (351 aa), FASTA scores: opt: 223, E(): 8.3e-07, (24.35% identity in 304 aa overlap); AAK46260|MT1988 epoxide hydrolase from Mycobacterium tuberculosis strain CDC1551 (356 aa), FASTA scores: opt: 223, E(): 8.4e-07, (40.7% identity in 113 aa overlap); P49323|PRXC_STRLI|CPO|CPOL non-heme chloroperoxidase (chloride peroxidase) from Streptomyces lividans (275 aa), FASTA scores: opt: 220, E(): 1e-06,(29.5% identity in 305 aa overlap); etc. Equivalent to AAK47599 Hydrolase, alpha/beta hydrolase family from Mycobacterium tuberculosis strain CDC1551 but shorter 24 aa. Start chosen by similarity, alternative with good RBS possible.; Possible non-heme haloperoxidase Hpx 3540745 hpx 887293 hpx Mycobacterium tuberculosis H37Rv Possible non-heme haloperoxidase Hpx NP_217687.1 3539846 R 83332 CDS NP_217688.1 15610308 888848 complement(3540882..3541364) 1 NC_000962.3 Rv3172c, (MTV014.16c), len: 160 aa. Hypothetical unknown protein.; Hypothetical protein 3541364 888848 Rv3172c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217688.1 3540882 R 83332 CDS NP_217689.1 15610309 888844 complement(3541443..3542045) 1 NC_000962.3 Rv3173c, (MTV014.17c), len: 200 aa. Probable transcriptional regulatory protein TetR family, similar to several bacterial putative regulatory proteins e.g. Q9EWI2|SC7H9.14 from Streptomyces coelicolor (195 aa),FASTA scores: opt: 319, E(): 1.7e-13, (34.55% identity in 195 aa overlap); O85695|3SCF60.04 from Streptomyces lividans and Streptomyces coelicolor (192 aa), FASTA scores: opt: 297, E(): 4.3e-12, (37.45% identity in 187 aa overlap); BAB50853|MLR4117 from Rhizobium loti (Mesorhizobium loti) (205 aa), FASTA scores: opt: 280, E(): 5.5e-11, (31.45% identity in 194 aa overlap); BAB53760|MLL8133 from Rhizobium loti (Mesorhizobium loti) (194 aa), FASTA scores: opt: 270, E(): 2.3e-10, (34.05% identity in 185 aa overlap); etc. Also similar to other regulators from Mycobacterium tuberculosis e.g. P96839|Rv3557c|MTCY06G11.04c (200 aa), FASTA scores: opt: 154, E(): 0.0013, (38.8% identity in 80 aa overlap). Contains probable helix-turn-helix motif from aa 39 to 60 (Score 1251, +3.45 SD). Similar to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Probable transcriptional regulatory protein (probably TetR/AcrR-family) 3542045 888844 Rv3173c Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR/AcrR-family) NP_217689.1 3541443 R 83332 CDS NP_217690.1 15610310 887989 3542138..3542845 1 NC_000962.3 Rv3174, (MTV014.18), len: 235 aa. Probable oxidoreductase short-chain dehyrogenase/reductase, similar to others e.g. Q9RPT7|sits from Streptomyces albus (223 aa), FASTA scores: opt: 654, E(): 6.1e-32, (49.3% identity in 215 aa overlap); Q9RI61|SCJ11.46 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 626, E(): 2.9e-30,(50.9% identity in 224 aa overlap); Q9A5Z1|CC2306 from Caulobacter crescentus (252 aa), FASTA scores: opt: 430,E(): 1.3e-18, (39.45% identity in 228 aa overlap); Q51641 insect-type dehydrogenase (249 aa), FASTA scores: opt: 301,E(): 5.7e-11, (38.3% identity in 188 aa overlap); Q9HXC9|PA3883 from Pseudomonas aeruginosa (276 aa), FASTA scores: opt: 296, E(): 1.2e-10, (29.55% identity in 247 aa overlap); etc. May belong to the short-chain dehydrogenases/reductases (SDR) family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable short-chain dehydrogenase/reductase 3542845 887989 Rv3174 Mycobacterium tuberculosis H37Rv Probable short-chain dehydrogenase/reductase NP_217690.1 3542138 D 83332 CDS NP_217691.1 15610311 887294 3542860..3544347 1 NC_000962.3 Rv3175, (MTV014.19), len: 495 aa. Possible amidase ,similar to others e.g. Q9F6D0|ZHUL enantiomer selective amidase from Streptomyces sp. R1128 (507 aa), FASTA scores: opt: 1328 ,E(): 7.5e-69, (44.5% identity in 492 aa overlap); BAB51815|MLR5350 probable amidase from Rhizobium loti (Mesorhizobium loti) (457 aa), FASTA scores: opt: 7487, E(): 1.3e-35, (35.9% identity in 482 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 532,E(): 3.2e-23, (32.05% identity in 471 aa overlap); etc. But also similar to glutamyl-tRNA amidotransferases who belong to amidase family e.g. Q9RTA9|DR1856 glutamyl-tRNA(GLN) amidotransferase, subunit A from Deinococcus radiodurans (482 aa), FASTA scores: opt: 560, E(): 8.2e-25, (30.6% identity in 513 aa overlap); Q9LCX3|GATA GLU/asp-tRNA amidotransferase subunit A from Thermus aquaticus (subsp. thermophilus) (471 aa), FASTA scores: opt: 558, E(): 1.1e-24, (30.85% identity in 486 aa overlap); Q49091|GATA_MORCA glutamyl-tRNA(GLN) amidotransferase subunit A from Moraxella catarrhalis (492 aa), FASTA scores: opt: 526, E(): 7.5e-23, (30.45% identity in 473 aa overlap); etc. Seems to belong to the amidase family. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible amidase (aminohydrolase) 3544347 887294 Rv3175 Mycobacterium tuberculosis H37Rv Possible amidase (aminohydrolase) NP_217691.1 3542860 D 83332 CDS YP_177938.1 57117068 888846 complement(3544344..3545300) 1 NC_000962.3 Rv3176c, (MTV014.20c), len: 318 aa. Probable mesT,epoxide hydrolase, similar to others e.g. O15007|PEG1|MEST|Q92571|O14973 MEST protein (mesoderm specific transcript (mouse) homolog) (similar to alpha/beta hydrolase fold) from Homo sapiens (Human) (335 aa), FASTA scores: opt: 348, E(): 6e-15, (32.15% identity in 280 aa overlap); AAH06639|Q07646 MEST protein from Mus musculus (Mouse) (335 aa), FASTA scores: opt: 342, E(): 1.4e-14,(31.45% identity in 280 aa overlap); Q9I8E7|MEST epoxide hydrolase from Fugu rubripes (Japanese pufferfish) (Takifugu rubripes) (326 aa), FASTA scores: opt: 322, E(): 2.7e-13, (29.55% identity in 301 aa overlap); Q9PUC9|PEG1|MEST epoxide hydrolase from Brachydanio rerio (Zebrafish) (Zebra danio) (344 aa), FASTA scores: opt: 322,E(): 2.8e-13, (32.35% identity in 207 aa overlap); Q9HYH6|PA3429 probable epoxide hydrolase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 258, E(): 3e-09,(29.85% identity in 288 aa overlap); O31243|ECHA epoxide hydrolase from Agrobacterium radiobacter (294 aa), FASTA scores: opt: 202, E(): 1.1e-05, (27.0% identity in 278 aa overlap); etc. Also similar to Q50599|Rv1834|MT1882|MTCY1A11.09c hypothetical 31.7 KDA protein from Mycobacterium tuberculosis (288 aa), FASTA scores: opt: 294, E(): 1.5e-11, (29.95% identity in 287 aa overlap). Equivalent to AAK47604 from Mycobacterium tuberculosis strain CDC1551 (339 aa) but shorter 21 aa. Similar to alpha/beta hydrolase fold. May belong to peptidase family S33. Note that previously known as lipS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable epoxide hydrolase MesT (epoxide hydratase) (arene-oxide hydratase) 3545300 mesT 888846 mesT Mycobacterium tuberculosis H37Rv Probable epoxide hydrolase MesT (epoxide hydratase) (arene-oxide hydratase) YP_177938.1 3544344 R 83332 CDS NP_217693.1 15610313 888766 3545447..3546307 1 NC_000962.3 Rv3177, (MTV014.21), len: 286 aa. Possible peroxidase (non-haem peroxidase), highly similar to Q9KJF9|W78 cultivar specificity protein (similar to alpha/beta hydrolase fold) W78 from Rhizobium leguminosarum (287 aa), FASTA scores: opt: 1059, E(): 2.3e-59, (61.4% identity in 272 aa overlap); BAB48728|MLL1328 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (286 aa),FASTA scores: opt: 746, E(): 1.1e-39, (43.25% identity in 282 aa overlap). Similar to nonheme chloroperoxidases and related esterases e.g. O73957|SAL lipolytic enzyme from Sulfolobus acidocaldarius (314 aa), FASTA scores: opt: 408,E(): 1.9e-18, (32.4% identity in 287 aa overlap); Q9AJM9|BIOH protein involved in biotin synthesis from Kurthia sp. 538-KA26 (267 aa), FASTA scores: opt: 324 ,E(): 3.2e-13, (30.0% identity in 250 aa overlap); Q9CBB1|ML2269 putative hydrolase (similar to alpha/beta hydrolase fold) from Mycobacterium leprae (265 aa); O05691|THCF_RHOER non-heme haloperoxidase from Rhodococcus erythropolis (similar to other bacterial non-heme BROMO- and chloro-peroxidases) (274 aa), FASTA scores: opt: 279, E(): 2.2e-10, (29.0% identity in 276 aa overlap); Q53540|est esterase (similar to alpha/beta hydrolase fold) from Pseudomonas putida (276 aa), FASTA scores: opt: 271, E(): 7.1e-10, (29.65% identity in 280 aa overlap); etc. Also similar to O06420|BPOC|Rv0554|MTCY25D10.33 hypothetical 28.3 KDA protein (similar to alpha/beta hydrolase fold) from M. tuberculosis (262 aa), FASTA scores: opt: 280 ,E(): 1.8e-10, (28.0% identity in 257 aa overlap). Equivalent to AAK47605 from Mycobacterium tuberculosis strain CDC1551 (300 aa) but shorter 14 aa. Similar to alpha/beta hydrolase fold. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible peroxidase (non-haem peroxidase) 3546307 888766 Rv3177 Mycobacterium tuberculosis H37Rv Possible peroxidase (non-haem peroxidase) NP_217693.1 3545447 D 83332 CDS NP_217694.1 15610314 888786 3546438..3546797 1 NC_000962.3 Rv3178, (MTV014.22), len: 119 aa. Hypothetical protein, with some similarity to other hypothetical bacterial proteins (principally mycobacterium and streptomyces proteins) e.g. P71854|Rv3547|MTCY03C7.09c from Mycobacterium tuberculosis strain H37Rv (151 aa), FASTA scores: opt: 310, E(): 2e-14, (40.5% identity in 116 aa overlap); Q9ZH81 from M. paratuberculosis (144 aa), FASTA scores: opt: 274, E(): 5.6e-12, (38.9% identity in 108 aa overlap); O85698|3SCF60.07 from Streptomyces lividans and Streptomyces coelicolor (149 aa), FASTA scores: opt: 235,E(): 2.7e-09, (35.2% identity in 108 aa overlap); Q10772|YF58_MYCTU|Rv1558|MT1609|MTCY48.07c (148 aa); Q9WX21|SCE68.11 from Streptomyces coelicolor (305 aa); etc. Equivalent to AAK47606 from Mycobacterium tuberculosis strain CDC1551 (171 aa) but shorter 52 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3546797 888786 Rv3178 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217694.1 3546438 D 83332 CDS NP_217695.1 15610315 887947 3547618..3548907 1 NC_000962.3 Rv3179, (MTV014.23), len: 429 aa. Conserved protein,highly similar to Q9KH61 putative ATP/GTP binding protein from Mycobacterium smegmatis (428 aa), FASTA scores: opt: 2466, E(): 1.5e-148, (89.7% identity in 428 aa overlap) (no article found on the NCBI web site (July 2001)); and to other hypothetical bacterial proteins e.g. O07781|Rv0597c|MTCY19H5.25 from M. tuberculosis (411 aa),FASTA scores: opt: 1031, E(): 8e-58, (41.5% identity in 417 aa overlap); BAB54715|MLR9349 from Rhizobium loti (Mesorhizobium loti) (435 aa), FASTA scores: opt: 365, E(): 1.1e-15, (31.75% identity in 416 aa overlap); etc. Equivalent to AAK47609 from Mycobacterium tuberculosis strain CDC1551 (454 aa) but shorter 25 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3548907 887947 Rv3179 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217695.1 3547618 D 83332 CDS NP_217696.1 15610316 887946 complement(3549254..3549688) 1 NC_000962.3 Rv3180c, (MTV014.24c), len: 144 aa. Hypothetical unknown ala-rich protein. Contains probable coiled-coil domain from aa 40 to 70. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical alanine rich protein 3549688 887946 Rv3180c Mycobacterium tuberculosis H37Rv Hypothetical alanine rich protein NP_217696.1 3549254 R 83332 CDS NP_217697.1 15610317 888787 complement(3549691..3550143) 1 NC_000962.3 Rv3181c, (MTV014.25c), len: 150 aa. Conserved protein, with some similarity to other mycobacterium proteins e.g. Q50718|YY07_MYCTU|Rv3407|MT3515|MTCY78.21c (99 aa), FASTA scores: opt: 123, E(): 0.25, (33.7% identity in 89 aa overlap); and O50412|Rv3385c|MTV004.43c (102 aa),FASTA scores: opt: 123, E(): 0.26, (39.7% identity in 68 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3550143 888787 Rv3181c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217697.1 3549691 R 83332 CDS NP_217698.1 15610318 888795 3550374..3550718 1 NC_000962.3 Rv3182, (MTV014.26), len: 114 aa. Hypothetical protein, with some similarity to other hypothetical bacterial proteins e.g. O53468|Rv2022c|MTV018.09c from M. tuberculosis (201 aa), FASTA scores: opt: 335, E(): 3.6e-16, (51.9% identity in 104 aa overlap); and Q9L3R6|ORF119 from Anabaena sp. strain PCC 7120 (119 aa),FASTA scores: opt: 250, E(): 1.6e-10, (42.1% identity in 95 aa overlap). Equivalent to AAK47614 from Mycobacterium tuberculosis strain CDC1551 (94 aa) but longer 20 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3550718 888795 Rv3182 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217698.1 3550374 D 83332 CDS NP_217699.1 15610319 888059 3550715..3551044 1 NC_000962.3 Rv3183, (MTV014.27), len: 109 aa. Possible transcriptional regulator, similar to others e.g. Q9S1D9|YPPCP1.08c from Yersinia pestis (99 aa), FASTA scores: opt: 119, E(): 0.47, (40.55% identity in 74 aa overlap); Q9X153|TM1330 from Thermotoga maritima (111 aa),FASTA scores: opt: 115, E(): 0.91, (40.35% identity in 57 aa overlap); P95258|Rv1956|MTCY09F9.08c (alias AAK46277 putative DNA-binding protein from strain CDC1551) (149 aa),FASTA scores: opt: 116, E(): 1, (42.25% identity in 71 aa overlap). Also similar to O53467|Rv2021c|MTV018.08c from Mycobacterium tuberculosis (101 aa), FASTA scores: opt: 214, E(): 5.8e-07, (43.0% identity in 107 aa overlap). Contains probable helix-turn-helix motif from aa 51 to 72 (Score 1803, +5.33 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible transcriptional regulatory protein 3551044 888059 Rv3183 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_217699.1 3550715 D 83332 CDS NP_217700.1 15610320 888796 3551281..3551607 1 NC_000962.3 Rv3184, (MTV014.28), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3184 and Rv3185,the sequence UUUUAAAG (directly upstream of Rv3185) maybe responsible for such a frameshifting event (see McAdam et al., 1990). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase for insertion sequence element IS6110 (fragment) 3551607 888796 Rv3184 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS6110 (fragment) NP_217700.1 3551281 D 83332 CDS NP_217701.2 448824801 887441 <3551556..3552542 1 NC_000962.3 Rv3185, (MTV014.29), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3184 and Rv3185, the sequence UUUUAAAG (directly upstream of Rv3185) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase 3552542 887441 Rv3185 Mycobacterium tuberculosis H37Rv Probable transposase NP_217701.2 <3551556 D 83332 CDS NP_217702.1 15610322 888024 3552764..3553090 1 NC_000962.3 Rv3186, (MTV014.30), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3186 and Rv3187,the sequence UUUUAAAG (directly upstream of Rv3187) maybe responsible for such a frameshifting event (see McAdam et al., 1990). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase for insertion sequence element IS6110 (fragment) 3553090 888024 Rv3186 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS6110 (fragment) NP_217702.1 3552764 D 83332 CDS NP_217703.2 448824802 887604 <3553039..3554025 1 NC_000962.3 Rv3187, (MTV014.31), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3186 and Rv3187, the sequence UUUUAAAG (directly upstream of Rv3187) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase 3554025 887604 Rv3187 Mycobacterium tuberculosis H37Rv Probable transposase NP_217703.2 <3553039 D 83332 CDS NP_217704.1 15610324 888103 3554298..3554645 1 NC_000962.3 Rv3188, (MTV014.32), len: 115 aa. Conserved hypothetical protein, with similarity to other proteins from Mycobacterium tuberculosis: Q10868|YJ90_MYCTU|Rv1990c|MT2044|MTCY39.29 hypothetical protein (113 aa), FASTA scores: opt: 184, E(): 8.1e-06,(28.45% identity in 109 aa overlap); and O06299|Rv0348|MTCY13E10.08 hypothetical protein (217 aa),FASTA scores: opt: 129, E(): 0.074, (30.0% identity in 100 aa overlap). Also some similarity with C-terminus of Q9XA59|SCGD3.19 putative two-component system response transcriptional regulator from Streptomyces coelicolor (218 aa), FASTA scores: opt: 114, E(): 0.76, (30.0% identity in 110 aa overlap) (for this one, no similarity exists in the N-terminal region with the N-terminus of other regulatory components of sensory transduction systems). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3554645 888103 Rv3188 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217704.1 3554298 D 83332 CDS NP_217705.1 15610325 888034 3554642..3555262 1 NC_000962.3 Rv3189, (MTV014.33), len: 206 aa. Conserved hypothetical protein, weakly similar to other proteins from Mycobacterium tuberculosis e.g. O86329|MBTE|Rv2380c|MTCY22H8.05 (1682 aa), FASTA scores: opt: 135, E(): 0.79, (27.8% identity in 187 aa overlap); and Q10869|YJ89_MYCTU|Rv1989c|MT2043MTCY39.30 (186 aa),FASTA scores: opt: 122, E(): 0.85, (32.25% identity in 93 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3555262 888034 Rv3189 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217705.1 3554642 D 83332 CDS NP_217706.1 15610326 888119 complement(3555422..3556687) 1 NC_000962.3 Rv3190c, (MTV014.34c), len: 421 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 3556687 888119 Rv3190c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217706.1 3555422 R 83332 CDS YP_007411905.1 448818032 14515873 3556855..3557064 1 NC_000962.3 Rv3190A, len: 69 aa. Conserved protein.; hypothetical protein 3557064 14515873 Rv3190A Mycobacterium tuberculosis H37Rv hypothetical protein YP_007411905.1 3556855 D 83332 CDS NP_217707.1 15610327 887628 complement(3557311..3558345) 1 NC_000962.3 Rv3191c, (MTV014.35c), len: 344 aa. Probable transposase, similar to many especially Q9K2N8 putative transposase from Pseudomonas aeruginosa (338 aa), FASTA scores: opt: 837, E(): 1.3e-43, (42.55% identity in 336 aa overlap); Q9RBF4 insertion sequence IS1088 from Alcaligenes eutrophus (Ralstonia eutropha) (342 aa), FASTA scores: opt: 823, E(): 9.2e-43, (43.05% identity in 337 aa overlap); and Q51379 putative transposase from Pseudomonas alcaligenes (338 aa), FASTA scores: opt: 818, E(): 1.8e-42, (42.35% identity in 333 aa overlap). Contains probable helix-turn-helix motif from aa 25 to 46 (Score 1968, +5.89 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable transposase 3558345 887628 Rv3191c Mycobacterium tuberculosis H37Rv Probable transposase NP_217707.1 3557311 R 83332 CDS NP_217708.1 15610328 887899 3559563..3560024 1 NC_000962.3 Rv3192, (MTV014.36), len: 153 aa. Conserved hypothetical ala- and pro-rich protein, with weak similarity to N-terminal half of several proteins e.g. Q11030|YD60_MYCTU|Rv1360|MT1405|MTCY02B10.24 hypothetical 37.3 KDA protein from Mycobacterium tuberculosis (340 aa),FASTA scores: opt: 245, E(): 3.7e-08, (33.1% identity in 157 aa overlap); O30260|AF2411 conserved hypothetical protein from Archaeoglobus fulgidus (363 aa), FASTA scores: opt: 144, E(): 0.072, (32.6% identity in 92 aa overlap); Q9ZA30|GRA-ORF29 putative FMN-dependent monooxygenase from Streptomyces violaceoruber (343 aa), FASTA scores: opt: 133, E(): 0.33, (25.15% identity in 159 aa overlap).; Conserved hypothetical alanine and proline-rich protein 3560024 887899 Rv3192 Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine and proline-rich protein NP_217708.1 3559563 D 83332 CDS NP_217709.1 15610329 888012 complement(3560194..3563172) 1 NC_000962.3 Rv3193c, (MTV014.37c), len: 992 aa. Probable conserved transmembrane protein, with hydrophobic N-terminal domain (~1-340 aa), highly similar to Q9CCM6|ML0644 putative integral membrane protein from Mycobacterium leprae (983 aa), FASTA scores: opt: 5421,E(): 0, (86.15% identity in 989 aa overlap); and O53609|Rv0064|MTV030.07 putative membrane protein from Mycobacterium tuberculosis strain H37Rv (979 aa), FASTA scores: opt: 3204, E(): 2.1e-142, (50.25% identity in 985 aa overlap). C-terminal part (709-990 aa) highly similar to O32904|MLCB1779.46 hypothetical 29.1 KDA protein from Mycobacterium leprae (277 aa), FASTA scores: opt: 1521,E(): 3.4e-64, (82.6% identity in 282 aa overlap). Also some similarity to hypothetical proteins generally transmembrane e.g. Q9FCI4|2SC3B6.28 from Streptomyces coelicolor (815 aa), FASTA scores: opt: 951, E(): 3.4e-37, (39.2% identity in 826 aa overlap); P72637|SLL1060 from Synechocystis sp. strain PCC 6803 (1032 aa), FASTA scores: opt: 938, E(): 1.6e-36, (29.95% identity in 855 aa overlap); O28851|AF1421 from Archaeoglobus fulgidus (880 aa), FASTA scores: opt: 526, E(): 2.6e-17, (28.05% identity in 970 aa overlap); etc.; Probable conserved transmembrane protein 3563172 888012 Rv3193c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217709.1 3560194 R 83332 CDS NP_217710.1 15610330 888062 complement(3563264..3564286) 1 NC_000962.3 Rv3194c, (MTV014.38c), len: 340 aa. Possible conserved secreted protein (N-terminal stretch hydrophobic), equivalent to Q9CCM7|ML0643 putative secreted protein from Mycobacterium leprae (340 aa), FASTA scores: opt: 1822, E(): 1.6e-102, (80.3% identity in 340 aa overlap). Also similar to other proteins e.g. Q9FCI6|2SC3B6.26 putative secreted protein from Streptomyces coelicolor (364 aa), FASTA scores: opt: 430,E(): 1.1e-18, (40.95% identity in 359 aa overlap); Q9S3Y5|SDRC SDRC protein from Streptomyces coelicolor (241 aa), FASTA scores: opt: 396, E(): 8.9e-17, (35.2% identity in 318 aa overlap) (similarity in part for this one); O34470|YLBL YLBL protein from Bacillus subtilis (350 aa),FASTA scores: opt: 385, E(): 5.6e-16, (27.7% identity in 350 aa overlap); etc.; Possible conserved secreted protein 3564286 888062 Rv3194c Mycobacterium tuberculosis H37Rv Possible conserved secreted protein NP_217710.1 3563264 R 83332 CDS NP_217711.1 15610331 888900 3564364..3565782 1 NC_000962.3 Rv3195, (MTV014.39), len: 472 aa. Hypothetical protein, equivalent to Q49746|ML0642|B1937_C3_231 hypothetical 50.3 KDA protein from Mycobacterium leprae (479 aa), FASTA scores: opt: 2503, E(): 1e-138, (79.35% identity in 475 aa overlap). Similar in part to Q9FCI9|2SC3B6.23c conserved hypothetical protein from Streptomyces coelicolor (487 aa), FASTA scores: opt: 1382,E(): 2.7e-73, (46.4% identity in 489 aa overlap); Q9X8I7|SCE9.14 hypothetical 41.2 KDA protein from Streptomyces coelicolor (375 aa), FASTA scores: opt: 319,E(): 2.4e-11, (25.6% identity in 383 aa overlap); etc.; hypothetical protein 3565782 888900 Rv3195 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217711.1 3564364 D 83332 CDS NP_217712.1 15610332 888903 3565788..3566687 1 NC_000962.3 Rv3196, (MTV014.40), len: 299 aa. Hypothetical protein, with some similarity to other hypothetical proteins e.g. Q9FCJ5|2SC3B6.17c putative secreted protein from Streptomyces coelicolor (442 aa), FASTA scores: opt: 233, E(): 3.5e-07, (29.9% identity in 261 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 3566687 888903 Rv3196 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217712.1 3565788 D 83332 CDS YP_177939.1 57117069 3205082 complement(3566696..3566896) 1 NC_000962.3 Rv3196A, len: 66 aa. Unknown protein.; hypothetical protein 3566896 3205082 Rv3196A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177939.1 3566696 R 83332 CDS NP_217713.1 15610333 887858 3567024..3568367 1 NC_000962.3 Rv3197, (MTV014.41), len: 447 aa. Probable conserved ATP-binding protein ABC transporter, highly similar to Mycobacterium leprae proteins: Q9CCM8|ML0640 hypothetical protein (473 aa), FASTA scores: opt: 2512, E(): 2.1e-140,(83.0% identity in 447 aa overlap). Interestingly, the N-terminal half (1-219 aa) corresponds to Q49747|ABC1|B1937_C3_233 ABC1 protein from Mycobacterium leprae (267 aa), FASTA scores: opt: 1276, E(): 6.3e-68,(88.6% identity in 219 aa overlap); and the C-terminal half (239-447 aa) corresponds to Q49745|B1937_C2_179 hypothetical 23.1 KDA protein (206 aa), FASTA scores: opt: 1138, E(): 6.5e-60, (77.05% identity in 209 aa overlap); two adjacent orfs from Mycobacterium leprae. Also highly similar to other proteins (generally ABC transporters) e.g. Q9FCJ6|2SC3B6.16c hypothetical 51.3 KDA protein from Streptomyces coelicolor (469 aa), FASTA scores: opt: 1340,E(): 1.8e-71, (45.9% identity in 449 aa overlap); O65576|ABC1AT ABC1 protein (alias Q9SBB2|T15B16.14|AT4G01660 putative ABC transporter) from Arabidopsis thaliana (Mouse-ear cress) (623 aa), FASTA scores: opt: 543, E(): 1.7e-24, (28.4% identity in 405 aa overlap); O27682|MTH1645 ABC transporter from Methanobacterium thermoautotrophicum (623 aa), FASTA scores: opt: 497, E(): 7.8e-22, (33.0% identity in 309 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable conserved ATP-binding protein ABC transporter 3568367 887858 Rv3197 Mycobacterium tuberculosis H37Rv Probable conserved ATP-binding protein ABC transporter NP_217713.1 3567024 D 83332 CDS YP_177940.1 57117070 3205083 complement(3568401..3568679) 1 NC_000962.3 Rv3197A, len: 92 aa. Probable whiB7 (alternate gene name: whmC), WhiB-like regulatory protein (see citation below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q49765|WHIB7|ML0639|B1937_F2_68 putative transcriptional regulator WHIB7 from Mycobacterium leprae (89 aa), FASTA scores: opt: 441, E(): 6.3e-24, (69.3% identity in 88 aa overlap). Similar to Q9FCJ8|2SC3B6.14 putative DNA-binding protein from Streptomyces coelicolor (122 aa), FASTA scores: opt: 348, E(): 2.2e-17, (57.7% identity in 78 aa overlap); Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa), FASTA scores: opt: 166,E(): 7.1e-05, (39.4% identity in 76 aa overlap); etc.; Probable transcriptional regulatory protein WhiB-like WhiB7 3568679 whiB7 3205083 whiB7 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein WhiB-like WhiB7 YP_177940.1 3568401 R 83332 CDS NP_217714.1 15610334 888902 complement(3569109..3571211) 1 NC_000962.3 Rv3198c, (MTV014.42c), len: 700 aa. Probable UvrD2,ATP dependent DNA helicase II (see citation below),equivalent to P53528|UVRD_MYCLE|VRD|UVRD2|ML0637|B1937_F1_27 probable DNA helicase II homolog from Mycobacterium leprae (717 aa),FASTA scores: opt: 3749, E(): 0, (82.85% identity in 706 aa overlap); and C-terminal half (466-700 aa) corresponds to Q49764|RECQ|B1937_F2_66 putative DNA helicase RECQ (242 aa), FASTA scores: opt: 1267, E(): 1.4e-69, (82.5% identity in 234 aa overlap); products of two adjacent ORFS in Mycobacterium leprae. Also similar to other DNA helicases e.g. Q9FCK0|2SC3B6.12 from Streptomyces coelicolor (785 aa), FASTA scores: opt: 1687, E(): 1.2e-94, (52.05% identity in 728 aa overlap); P71561|CRA|IVRD|Rv0949|MT0976|MTCY10D7.25c ATP-dependent DNA helicase PCRA from Mycobacterium tuberculosis (771 aa),FASTA scores: opt: 715, E(): 1e-35, (34.1% identity in 710 aa overlap); Q9CD72|PCRA_MYCLE|UVRD|ML0153 ATP-dependent DNA helicase PCRA from Mycobacterium leprae (778 aa), FASTA scores: opt: 687, E(): 5.1e-34, (32.0% identity in 719 aa overlap); O83991|TP1028 DNA helicase II (UVRD) from Treponema pallidum (670 aa), FASTA scores: opt: 652, E(): 6e-32, (30.25% identity in 671 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UVRD subfamily of helicases.; Probable ATP-dependent DNA helicase II UvrD2 3571211 uvrD2 888902 uvrD2 Mycobacterium tuberculosis H37Rv Probable ATP-dependent DNA helicase II UvrD2 NP_217714.1 3569109 R 83332 CDS YP_177941.1 57117071 3205084 3571335..3571589 1 NC_000962.3 Rv3198A, len: 84 aa. Possible glutaredoxin protein ,highly similar to Q9FCK1|2SC3B6.11c putative glutaredoxin-like protein from Streptomyces coelicolor (80 aa), FASTA scores: opt: 293, E(): 2.2e-14, (55.15% identity in 78 aa overlap); and Q9RSN9|DR2085 putative glutaredoxin from Deinococcus radiodurans (81 aa), FASTA scores: opt: 198, E(): 1.2e-07, (53.55% identity in 56 aa overlap). Also similar to several hypothetical bacterial proteins e.g. Q9X8C2|SCE36.09 hypothetical 13.0 KDA protein from Streptomyces coelicolor (114 aa), FASTA scores: opt: 181,E(): 2.6e-06, (44.45% identity in 72 aa overlap).; Possible glutaredoxin protein 3571589 3205084 Rv3198A Mycobacterium tuberculosis H37Rv Possible glutaredoxin protein YP_177941.1 3571335 D 83332 CDS NP_217715.1 15610335 887860 complement(3571602..3572543) 1 NC_000962.3 Rv3199c, (MTV014.43)c, len: 313 aa. Probable nudC,NADH pyrophosphatase, similar in particular to Q9CXN4|4933433B15RIK from Mus musculus (Mouse) (356 aa),FASTA scores: opt: 493, E(): 7.4e-24, (39.65% identity in 232 aa overlap); Q9ABG1|CC0266 mutt/NUDIX family protein from Caulobacter crescentus (313 aa), FASTA scores: opt: 479, E(): 5.1e-23, (38.3% identity in 222 aa overlap); O86062|NUDC_PSEAE|NUDC|PA1823 NADH pyrophosphatase from Pseudomonas aeruginosa (278 aa), FASTA scores: opt: 371,2 E(): 3e-16, (43.15% identity in 153 aa overlap); Q9RV62|NUDC_DEIRA|NUDC|DR1168 NADH pyrophosphatase from Deinococcus radiodurans (280 aa), FASTA scores: opt: 363,E(): 9.6e-16, (34.45% identity in 270 aa overlap); etc. Caution: equivalent to AAK47636 from Mycobacterium tuberculosis strain CDC1551 (386 aa) but shorter 72 aa. Contains PS00893 mutT domain signature. Belongs to the NUDIX hydrolase family, NUDC subfamily. Cofactor: requires divalent ions: manganese or magnesium.; Probable NADH pyrophosphatase NudC (NAD+ diphosphatase) (NAD+ pyrophosphatase) (NADP pyrophosphatase) 3572543 nudC 887860 nudC Mycobacterium tuberculosis H37Rv Probable NADH pyrophosphatase NudC (NAD+ diphosphatase) (NAD+ pyrophosphatase) (NADP pyrophosphatase) NP_217715.1 3571602 R 83332 CDS NP_217716.1 15610336 887543 complement(3572602..3573669) 1 NC_000962.3 Rv3200c, (MTV014.44c), len: 355 aa. Possible transmembrane cation transporter, similar to many transmembrane proteins and putative potassium channels e.g. Q9XA52|SCGD3.27C putative membrane protein from Streptomyces coelicolor (365 aa), FASTA scores: opt: 1022,E(): 2.6e-53, (49.85% identity in 325 aa overlap); Q9RRZ3|DR2336 putative potassium channel from Deinococcus radiodurans (320 aa), FASTA scores: opt: 436, E(): 1e-18,(30.9% identity in 304 aa overlap); O28600|AF1673 putative potassium channel from Archaeoglobus fulgidus (314 aa),FASTA scores: opt: 363, E(): 2.1e-14, (27.2% identity in 309 aa overlap); Q57604|Y13B_METJAMJ0138.1|MJ0138.1 putative potassium channel from Methanococcus jannaschii (333 aa), FASTA scores: opt: 356, E(): 5.7e-14, (26.0% identity in 281 aa overlap); P73132|SLL0993 potassium channel from Synechocystis sp. strain PCC 6803 (365 aa),FASTA scores: opt: 330, E(): 2.1e-12, (27.8% identity in 324 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible transmembrane cation transporter 3573669 887543 Rv3200c Mycobacterium tuberculosis H37Rv Possible transmembrane cation transporter NP_217716.1 3572602 R 83332 CDS NP_217717.1 15610337 887663 complement(3573731..3577036) 1 NC_000962.3 Rv3201c, (MTV014.45c), len: 1101 aa. Probable ATP-dependent DNA helicase, similar to others e.g. Q9FCK4|2SC3B6.08 from Streptomyces coelicolor (1222 aa),FASTA scores: opt: 1209, E(): 5.4e-63, (38.45% identity in 1199 aa overlap); P71561|PCRA_MYCTU|CRA|IVRD|Rv0949|MT0976|MTCY10D7.25c from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 403, E(): 6.5e-16, (28.15% identity in 717 aa overlap); Q9FCK5|2SC3B6.07 from Streptomyces coelicolor (1159 aa),FASTA scores: opt: 349, E(): 1.3e-12, (29.2% identity in 1144 aa overlap); Q9L3M1|UVRD from Prochlorococcus sp. (512 aa; fragment), FASTA scores: opt: 290, E(): 2e-09, (27.95% identity in 479 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable ATP-dependent DNA helicase 3577036 887663 Rv3201c Mycobacterium tuberculosis H37Rv Probable ATP-dependent DNA helicase NP_217717.1 3573731 R 83332 CDS NP_217718.1 15610338 887574 complement(3577033..3580200) 1 NC_000962.3 Rv3202c, (MTCY07D11.24, MTV014.46c), len: 1055 aa. Possible ATP-dependent DNA helicase, showing some similarity to UvrD proteins e.g. Q9FCK5|2SC3B6.07 putative ATP-dependent DNA helicase from Streptomyces coelicolor (1159 aa), FASTA scores: opt: 666, E(): 1e-29, (34.5% identity in 1154 aa overlap); Q9L7T3|UVRD|PA5443 mismatch repair protein MUTU (DNA helicase II) from Pseudomonas aeruginosa (728 aa), FASTA scores: opt: 239, E(): 7.3e-06,(23.8% identity in 677 aa overlap) (no similarity in C-terminal part for this one); etc. C-terminal region similar to Q9FDU2|ORF3 ORF3 protein (fragment) from Streptomyces griseus (551 aa), FASTA scores: opt: 800, E(): 1.7e-37, (36.2% identity in 525 aa overlap); and Q9ZG15 hypothetical 35.5 KDA protein from Rhodococcus erythropolis (323 aa), FASTA scores: opt: 232, E(): 9.7e-06, (28.55% identity in 266 aa overlap).; Possible ATP-dependent DNA helicase 3580200 887574 Rv3202c Mycobacterium tuberculosis H37Rv Possible ATP-dependent DNA helicase NP_217718.1 3577033 R 83332 CDS NP_217719.1 15610339 888133 3580638..3581312 1 NC_000962.3 Rv3203, (MTCY07D11.23c), len: 224 aa. Possible lipV,hydrolase lipase, showing some similarity to other lipases e.g. Q9JSN0|NMA2216 putative hydrolase from Neisseria meningitidis (serogroup A) (312 aa), FASTA scores: opt: 192, E(): 0.00016, (45.2% identity in 73 aa overlap); Q9RK95|SCF1.09 putative hydrolase from Streptomyces coelicolor (258 aa), FASTA scores: opt: 188, E(): 0.00024,(30.1% identity in 226 aa overlap); Q9KZC3|SC6F7.19c putative lipase from Streptomyces coelicolor (269 aa),FASTA scores: opt: 179, E(): 0.00086, (36.35% identity in 121 aa overlap); etc. Equivalent to AAK47641 Hydrolase,alpha/beta hydrolase family from Mycobacterium tuberculosis strain CDC1551 (261 aa) but shorter 37 aa. Contains serine active site signature of lipases (PS00120).; Possible lipase LipV 3581312 lipV 888133 lipV Mycobacterium tuberculosis H37Rv Possible lipase LipV NP_217719.1 3580638 D 83332 CDS NP_217720.1 15610340 888132 3581315..3581620 1 NC_000962.3 Rv3204, (MTCY07D11.22c), len: 101 aa. Possible DNA methyltransferase, similar to many hypothetical bacteriel proteins and methyltransferases e.g. Q9KT40|VC1065 methylated-DNA--protein-cysteine methyltransferase-related protein from Vibrio cholerae (100 aa), FASTA scores: opt: 170, E(): 2.8e-05, (34.35% identity in 99 aa overlap); Q9UTN9|SPAC1250.04c putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (108 aa), FASTA scores: opt: 161, E(): 0.00013, (36.65% identity in 101 aa overlap); Q9YDF4|APE0959 175 AA long hypothetical methylated-DNA--protein-cysteine methyltransferase from Aeropyrum pernix (175 aa), FASTA scores: opt: 144, E(): 0.003, (37.95% identity in 87 aa overlap); Q50855 putative methylguanine-DNA methyltransferase from Myxococcus xanthus (147 aa), FASTA scores: opt: 141, E(): 0.0041, (37.65% identity in 93 aa overlap); etc.; Possible DNA-methyltransferase (modification methylase) 3581620 888132 Rv3204 Mycobacterium tuberculosis H37Rv Possible DNA-methyltransferase (modification methylase) NP_217720.1 3581315 D 83332 CDS NP_217721.1 15610341 888877 complement(3581627..3582505) 1 NC_000962.3 Rv3205c, (MTCY07D11.21), len: 292 aa. Conserved protein, highly similar to Q9CCG7|ML0818 hypothetical protein from Mycobacterium leprae (297 aa), FASTA scores: opt: 1745, E(): 9.1e-98, (87.3% identity in 291 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3582505 888877 Rv3205c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217721.1 3581627 R 83332 CDS YP_177942.1 57117072 888871 complement(3582532..3583710) 1 NC_000962.3 Rv3206c, (MTCY07D11.20), len: 392 aa. Probable moeB1, molybdopterin cofactor biosynthesis protein,equivalent to Q9CCG8|MOEZ|ML0817 protein probably involved in molybdopterin biosynthesis from Mycobacterium leprae (395 aa), FASTA scores: opt: 2285, E(): 3.3e-130, (86.45% identity in 391 aa overlap.) Very similar to members of the HESA/MOEB/THIF family e.g. Q9FCL0|2SC3B6.02 putative sulfurylase from Streptomyces coelicolor (392 aa), FASTA scores: opt: 1776, E(): 1.4e-99, (65.3% identity in 395 aa overlap); Q9XC37|PDTORFF MOEB-like protein (putative sulfurylase) from Pseudomonas stutzeri (Pseudomonas perfectomarina) (391 aa), FASTA scores: opt: 1526, E(): 1.5e-84, (59.1% identity in 391 aa overlap); O54307|MPT|MOEB MPT-synthase sulfurylase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (391 aa), FASTA scores: opt: 1309, E(): 1.8e-71, (52.95% identity in 387 aa overlap); P74344|MOEB|SLL1536 molybdopterin biosynthesis MOEB protein from Synechocystis sp. strain PCC 6803 (392 aa), FASTA scores: opt: 1308, E(): 2e-71, (50.65% identity in 397 aa overlap); etc. Also highly similar to O05792|MOEB2|Rv3116|MTCY164.26 putative molybdenum cofactor biosynthesis protein from Mycobacterium tuberculosis (389 aa), FASTA scores: opt: 1440, E(): 2.3e-79, (57.25% identity in 386 aa overlap). Has hydrophobic segment from ~45-71. Belongs to the HesA/MoeB/ThiF FAMILY. Note that previously known as moeZ. Thought to be differentially expressed within host cells (see citation below).; Probable molybdenum cofactor biosynthesis protein MoeB1 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) 3583710 moeB1 888871 moeB1 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein MoeB1 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) YP_177942.1 3582532 R 83332 CDS NP_217723.1 15610343 888874 complement(3583801..3584658) 1 NC_000962.3 Rv3207c, (MTCY07D11.19), len: 285 aa. Conserved protein, highly similar but shorter (57 aa) to Q9CCG9|ML0816 hypothetical protein from Mycobacterium leprae (341 aa), FASTA scores: opt: 1676, E(): 9.7e-96,(81.0% identity in 284 aa overlap). Also similar to C-terminus of Q9FBI6|SCP8.36 hypothetical protein from Streptomyces coelicolor (559 aa), FASTA scores: opt: 426,E(): 8.4e-19, (37.35% identity in 281 aa overlap); and similar to other hypothetical proteins (generally membrane proteins) e.g. Q9K456|SC2H12.28C putative membrane protein from Streptomyces coelicolor (314 aa), FASTA scores: opt: 341, E(): 8.8e-14, (29.75% identity in 296 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142).; hypothetical protein 3584658 888874 Rv3207c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217723.1 3583801 R 83332 CDS NP_217724.1 15610344 887905 3585004..3585690 1 NC_000962.3 Rv3208, (MTCY07D11.18c), len: 228 aa. Probable transcriptional regulator, TetR family, equivalent to Q9CCH0|ML0815 putative TetR-family transcriptional regulator from Mycobacterium leprae (228 aa), FASTA scores: opt: 1248, E(): 1.4e-74, (82.4% identity in 227 aa overlap). Also highly similar to Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa), FASTA scores: opt: 629, E(): 4e-34,(45.8% identity in 203 aa overlap); Q9KIL9|F58R F58R (fragment) from Streptomyces coelicolor A3(2) (149 aa),FASTA scores: opt: 497, E(): 1.3e-25, (50.35% identity in 147 aa overlap); Q9K3T5|SCE66.08 putative TetR-family transcriptional regulator from Streptomyces coelicolor (225 aa), FASTA scores: opt: 344, E(): 1.8e-15, (31.15% identity in 212 aa overlap); Q9RYK4|DRA0308 transcriptional regulator, TetR family from Deinococcus radiodurans (239 aa), FASTA scores: opt: 290, E(): 6.5e-12, (30.5% identity in 223 aa overlap); etc. And also similar to Mycobacterium tuberculosis proteins P96381|Rv1019|MTCY10G2.30c hypothetical 21.7 KDA protein (197 aa), FASTA scores: opt: 356, E(): 2.7e-16, (34.4% identity in 189 aa overlap); MTV034_4; MTY07A7A_3; MTV032_1; MTCY07A7_12; etc. Contains probable helix-turn-helix motif at aa 60-81 (Score 1517,+4.35 SD). Similar to the TetR/AcrR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably TetR-family) 3585690 887905 Rv3208 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR-family) NP_217724.1 3585004 D 83332 CDS YP_177943.1 57117073 3205107 complement(3585677..3585949) 1 NC_000962.3 Rv3208A, len: 90 aa. TB9.4, conserved protein (see citations below), equivalent to Q9CCH1|ML0814 hypothetical protein from Mycobacterium leprae (82 aa), FASTA scores: opt: 411, E(): 1.8e-22, (81.0% identity in 79 aa overlap). Also similar, but shorter in N-terminus, to Q9FBI9|SCP8.32c putative ATP-binding protein from Streptomyces coelicolor (94 aa), FASTA scores: opt: 246, E(): 8.1e-11, (53.4% identity in 73 aa overlap); Q9DGP6 (alias Q9DGP4) glutamate decarboxylase 67 KDA isoform (fragment) from Alepocephalus bairdii (182 aa), FASTA scores: opt: 100, E(): 2.6, (35.3% identity in 85 aa overlap). Corresponds to Statens Serum Institute antigen, CYP10 TB9.4. Has N-terminal sequence,vevkigitdsprelv.; Conserved protein TB9.4 3585949 TB9.4 3205107 TB9.4 Mycobacterium tuberculosis H37Rv Conserved protein TB9.4 YP_177943.1 3585677 R 83332 CDS NP_217725.1 15610345 888875 3586274..3586834 1 NC_000962.3 Rv3209, (MTCY07D11.17c), len: 186 aa. Conserved hypothetical thr-, pro-rich protein, equivalent (but shorter 36 aa in N-terminus) to Q9CCH2|ML0813 putative membrane protein from Mycobacterium leprae (195 aa), FASTA scores: opt: 508, E(): 1.4e-15, (58.4% identity in 185 aa overlap). Also some similarity with Q10390|MMS3_MYCTU|MMPS3|Rv2198c|MT2254|MTCY190.09c probable conserved transmembrane transport protein from M. tuberculosis (299 aa), FASTA scores: opt: 339, E(): 3.7e-08, (35.0% identity in 180 aa overlap); and Q9CCE9|MMPS3|ML0877 putative membrane protein from Mycobacterium leprae (293 aa), FASTA scores: opt: 272, E(): 2.8e-05, (36.4% identity in 173 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Conserved hypothetical threonine and proline rich protein 3586834 888875 Rv3209 Mycobacterium tuberculosis H37Rv Conserved hypothetical threonine and proline rich protein NP_217725.1 3586274 D 83332 CDS NP_217726.1 15610346 888876 complement(3586844..3587539) 1 NC_000962.3 Rv3210c, (MTCY07D11.16), len: 231 aa. Conserved protein, similar (but N-terminus shorter) to Q9FBJ1|SCP8.30 conserved hypothetical protein from Streptomyces coelicolor (260 aa), FASTA scores: opt: 599, E(): 1.1e-30, (42.5% identity in 233 aa overlap); and some similarity to Q9RRV1|DR2384 phenylacetic acid degradation protein PAAC from Deinococcus radiodurans (263 aa), FASTA scores: opt: 129, E(): 0.43, (27.9% identity in 172 aa overlap); and Q9F621 FLGK protein from Rhizobium meliloti (Sinorhizobium meliloti) (472 aa).; hypothetical protein 3587539 888876 Rv3210c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217726.1 3586844 R 83332 CDS NP_217727.1 15610347 888840 3587798..3589381 1 NC_000962.3 Rv3211, (MTCY07D11.15c), len: 527 aa. Probable rhlE,ATP-dependent RNA helicase, equivalent (but shorter 22 aa) to Q9CCH3|RHLE|ML0811 putative ATP-dependent RNA helicase from Mycobacterium leprae (544 aa), FASTA scores: opt: 2497, E(): 8.7e-131, (74.75% identity in 531 aa overlap). Also highly similar to other RNA helicases e.g. Q9FBJ2|SCP8.29c from Streptomyces coelicolor (879 aa),FASTA scores: opt: 1458, E(): 3.6e-73, (52.5% identity in 522 aa overlap); Q9DF36 from Xenopus laevis (African clawed frog) (800 aa), FASTA scores: opt: 792, E(): 2.3e-36,(37.15% identity in 385 aa overlap); Q99Z38|dead|SPY1415 from Streptococcus pyogenes (759 aa), FASTA scores: opt: 779, E(): 1.1e-35, (37.1% identity in 380 aa overlap); P33906|dead|CSDA from Klebsiella pneumoniae (642 aa), FASTA scores: opt: 768, E(): 4e-35, (43.4% identity in 387 aa overlap); etc. Contains ATP/GTP-binding site motif A (PS00017) and dead-box subfamily ATP-dependent helicases signature (PS00039). Similar to dead/DEAH box helicase family and similar to helicase C-terminal domain.; Probable ATP-dependent RNA helicase RhlE 3589381 rhlE 888840 rhlE Mycobacterium tuberculosis H37Rv Probable ATP-dependent RNA helicase RhlE NP_217727.1 3587798 D 83332 CDS NP_217728.1 15610348 887931 3589394..3590617 1 NC_000962.3 Rv3212, (MTCY07D11.14c), len: 407 aa. Conserved ala-, val-rich protein, equivalent to Q9CCH4|ML0810 putative membrane protein from Mycobacterium leprae (407 aa), FASTA scores: opt: 2158, E(): 5.3e-119, (79.85% identity in 407 aa overlap). Weak similarity to several eukaryotic transcription factors e.g. P08393|ICP0_HSV11|ICP0|IE110 trans-acting transcriptional protein from Herpes simplex virus (type 1 / strain 17) (775 aa), FASTA scores: opt: 115, E(): 2, (26.9% identity in 334 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al.,2008).; Conserved alanine valine rich protein 3590617 887931 Rv3212 Mycobacterium tuberculosis H37Rv Conserved alanine valine rich protein NP_217728.1 3589394 D 83332 CDS NP_217729.1 15610349 888896 complement(3590692..3591492) 1 NC_000962.3 Rv3213c, (MTCY07D11.13), len: 266 aa. Possible soj/parA-related protein, very similar in particular to Soj/ParA proteins (and relatives) from Bacillus subtilis that inhibit the initiation of sporulation by preventing phosphorylation of Spo0A (see Quisel & Grossman 2000) e.g. Q9S228|SCI51.12c from Streptomyces coelicolor (340 aa),FASTA scores: opt: 746, E(): 1.6e-40, (48.2% identity in 249 aa overlap); Q9HT11|SOJ|PA5563 from Pseudomonas aeruginosa (262 aa), FASTA scores: opt: 649, E(): 2.1e-34,(42.2% identity in 256 aa overlap); Q9PB62|XF2282 from Xylella fastidiosa (264 aa), FASTA scores: opt: 624, E(): 8.3e-33, (42.25% identity in 251 aa overlap); Q9K5N0|SOJ_BACHD|SOJ|BH4058 from Bacillus halodurans (253 aa), FASTA scores: opt: 621, E(): 1.2e-32, (41.55% identity in 248 aa overlap); P37522|SOJ_BACSU (253 aa), FASTA scores: opt: 620, E(): 1.4e-32, (41.65% identity in 245; etc. Also similar to various mycobacterial proteins: U00021_10 from Mycobacterium leprae, MTCI125_29 from Mycobacterium tuberculosis, MLCB1351_6 from Mycobacterium leprae, MTV028_9c|Rv3918c|para probable chromosome partitioning protein from Mycobacterium tuberculosis,MSGDNAB_18 from Mycobacterium leprae. Seems to belong to the para family.; Possible SOJ/para-related protein 3591492 888896 Rv3213c Mycobacterium tuberculosis H37Rv Possible SOJ/para-related protein NP_217729.1 3590692 R 83332 CDS YP_177944.1 57117074 888830 3591646..3592257 1 NC_000962.3 Rv3214, (MTCY07D11.12c), len: 203 aa. Possible gpm2,phosphoglycerate mutase, similar to many mutases especially phosphoglycerate mutases e.g. Q9F3H5|2SCC13.14c putative mutase from Streptomyces coelicolor (198 aa), FASTA scores: opt: 487, E(): 4.4e-25, (42.25% identity in 194 aa overlap); BAB49378|MLL2186 probable phosphoglycerate mutase from Rhizobium loti (Mesorhizobium loti) (193 aa), FASTA scores: opt: 423, E(): 7e-21, (41.2% identity in 182 aa overlap); Q9RKV8|SC9G1.08c putative phosphatase from Streptomyces coelicolor (199 aa), FASTA scores: opt: 419,E(): 1.3e-20, (41.1% identity in 185 aa overlap); Q9RDL0|SCC123.14c putative phosphoglycerate mutase from Streptomyces coelicolor (223 aa), FASTA scores: opt: 240,E(): 8.8e-09, (36.9% identity in 168 aa overlap); Q9X194|TM1374 phosphoglycerate mutase from Thermotoga maritima (201 aa), FASTA scores: opt: 218, E(): 2.3e-07,(33.15% identity in 202 aa overlap); etc. But N-terminus also similar to Q9CCH5|ENTC|ML0808 putative isochorismate synthase from Mycobacterium leprae (577 aa), FASTA scores: opt: 346, E(): 2.1e-15, (55.05% identity in 109 aa overlap). N-terminus shows also some similarity with other M. tuberculosis proteins e.g. MTCY427.09c; MTCY20G9.15; MTCY428.28. Equivalent to AAK47652 from Mycobacterium tuberculosis strain CDC1551 (228 aa) but shorter 25 aa. Note that previously known as entD.; Possible phosphoglycerate mutase Gpm2 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) 3592257 gpm2 888830 gpm2 Mycobacterium tuberculosis H37Rv Possible phosphoglycerate mutase Gpm2 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) YP_177944.1 3591646 D 83332 CDS NP_217731.1 15610351 888824 3592254..3593372 1 NC_000962.3 Rv3215, (MTCY07D11.11c), len: 372 aa. Probable entC,isochorismate synthase, equivalent to Q9CCH5|ENTC|ML0808 putative isochorismate synthase from Mycobacterium leprae (577 aa), FASTA scores: opt: 1817, E(): 5.5e-105, (73.5% identity in 366 aa overlap). Also similar to others e.g. Q9F639|MXCD protein involved in myxochelin-type iron chelator biosynthesis (see citation below) from Stigmatella aurantiaca (408 aa), FASTA scores: opt: 893, E(): 6.2e-48,(41.6% identity in 382 aa overlap); P45744|DHBC_BACSU isochorismate synthase from Bacillus subtilis (398 aa),FASTA scores: opt: 883, E(): 2.5e-47, (40.45% identity in 393 aa overlap); Q9KI93|CSBC isochorismate synthase (fragment) from Azotobacter vinelandii (361 aa), FASTA scores: opt: 794, E(): 7.6e-42, (45.65% identity in 298 aa overlap); and the two Escherichia coli proteins AAG54928|ENTC (alias BAB34055|ECS0632) isochorismate hydroxymutase 2 from Escherichia coli strain O157:H7 (391 aa), FASTA scores: opt: 744, E(): 1e-38, (38.8% identity in 340 aa overlap); P10377|ENTC|B0593 isochorismate synthase from Escherichia coli strain K12 (391 aa), FASTA scores: opt: 744, E(): 1e-38, (38.8% identity in 340 aa overlap); etc. Stronger similarity to Escherichia coli entC. Also similar to MTCY253.35.; Probable isochorismate synthase EntC (isochorismate hydroxymutase) (enterochelin biosynthesis) 3593372 entC 888824 entC Mycobacterium tuberculosis H37Rv Probable isochorismate synthase EntC (isochorismate hydroxymutase) (enterochelin biosynthesis) NP_217731.1 3592254 D 83332 CDS NP_217733.1 15610353 888843 complement(3593804..3594235) 1 NC_000962.3 Rv3217c, (MTCY07D11.09), len: 143 aa. Probable conserved integral membrane protein, equivalent (highly similar but shorter 30 aa) to Q9CCH6|ML0806 putative membrane protein from Mycobacterium leprae (173 aa). Also similar to others e.g. Q9F3L9|2SC7G11.04 putative integral membrane protein from Streptomyces coelicolor (152 aa),FASTA scores: opt: 177, E(): 0.00024, (33.8% identity in 136 aa overlap). And shows similarity to O34238|MVIN|VC0680 virulence factor MVIN homolog from Vibrio (525 aa), FASTA scores: opt: 126, E(): 0.97, (30.9% identity in 68 aa overlap). First GTG taken. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Probable conserved integral membrane protein 3594235 888843 Rv3217c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217733.1 3593804 R 83332 CDS NP_217734.1 15610354 888906 3594468..3595433 1 NC_000962.3 Rv3218, (MTCY07D11.08c), len: 321 aa. Conserved protein, similar to several hypothetical bacterial proteins e.g. Q9F3M0|2SC7G11.03c from Streptomyces coelicolor (322 aa), FASTA scores: opt: 694, E(): 4.2e-35, (39.95% identity in 328 aa overlap); Q9A0J4|SPY0752 from Streptomyces pyogenes (340 aa), FASTA scores: opt: 187, E(): 0.00033,(30.5% identity in 141 aa overlap); O31502|YERQ from Bacillus subtilis (303 aa), FASTA scores: opt: 184, E(): 0.00045, (34.15% identity in 126 aa overlap); etc.; hypothetical protein 3595433 888906 Rv3218 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217734.1 3594468 D 83332 CDS NP_217735.1 15610355 887980 3595713..3595967 1 NC_000962.3 Rv3219, (MTCY07D11.07c), len: 84 aa. WhiB1 (alternate gene name: whmE), WhiB-like regulatory protein (see Hutter and Dick, 1999), similar to WhiB paralogue of Streptomyces coelicolor. Equivalent to Q9CCH7|WHIB1|ML0804 putative transcriptional regulator from Mycobacterium leprae (84 aa), FASTA scores: opt: 580, E(): 3.5e-35,(95.25% identity in 84 aa overlap). Highly similar to several e.g. Q9X952|WBLE developmental regulatory protein WhiB-paralog from Streptomyces coelicolor (85 aa), FASTA scores: opt: 477, E(): 9.2e-28, (75.3% identity in 81 aa overlap); Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa), FASTA scores: opt: 383,E(): 6.1e-21, (60.75% identity in 79 aa overlap); Q9K4K8|SC5F8.16c from Streptomyces coelicolor (83 aa),FASTA scores: opt: 346, E(): 2.5e-18, (54.75% identity in 84 aa overlap); etc.; Transcriptional regulatory protein WhiB-like WhiB1 Contains [4FE-4S]2+ cluster 3595967 whiB1 887980 whiB1 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein WhiB-like WhiB1 Contains [4FE-4S]2+ cluster NP_217735.1 3595713 D 83332 CDS NP_217736.1 15610356 888801 complement(3596029..3597534) 1 NC_000962.3 Rv3220c, (MTCY07D11.06), len: 501 aa. Probable sensor (probably histidine kinase), equivalent to Q9CCH8|ML0803 putative two-component system sensor kinase from Mycobacterium leprae (500 aa). Similar to others e.g. Q9F3M1|2SC7G11.01 putative histidine kinase (fragment) from Streptomyces coelicolor (372 aa), FASTA scores: opt: 1038,E(): 7.4e-56, (48.95% identity in 380 aa overlap); Q9A3K5|CC3198 sensor histidine kinase from Caulobacter crescentus (327 aa), FASTA scores: opt: 311, E(): 1.2e-11,(33.35% identity in 201 aa overlap) (similarity only in C-terminal part for this one); Q9A2T2|CC3474 putative sensor histidine kinase from Caulobacter crescentus (547 aa); etc. C-terminal half shows similarity to many sensor proteins, that respond to various stimuli from Methanobacterium thermoautotrophicum e.g. O26568|MTH468 sensory transduction histidine kinase (554 aa), FASTA scores: opt: 425, E(): 2.1e-18, (34.0% identity in 244 aa overlap); O26546|MTH446 sensory transduction regulatory protein (583 aa), FASTA scores: opt: 380, E(): 1.2e-15,(37.15% identity in 202 aa overlap); O26913|MTH823 sensory transduction regulatory protein (677 aa), FASTA scores: opt: 375, E(): 2.7e-15, (35.4% identity in 195 aa overlap); etc. Seems similar to other prokaryotic sensory transduction histidine kinases.; Probable two component sensor kinase 3597534 888801 Rv3220c Mycobacterium tuberculosis H37Rv Probable two component sensor kinase NP_217736.1 3596029 R 83332 CDS NP_217737.1 15610357 888096 complement(3597551..3597766) 1 NC_000962.3 Rv3221c, (MTCY07D11.05), len: 71 aa. TB7.3,Biotinylated protein (see citations below), equivalent (appears to have one additional residue) to Q9CCH9|ML0802|BTB7_MYCLE biotinylated protein TB7.3 homolog from Mycobacterium leprae (70 aa), FASTA scores: opt: 367,E(): 4e-18, (90.0% identity in 70 aa overlap); Q9XCD6|BTB7_MYCSM biotinylated protein TB7.3 homolog from Mycobacterium smegmatis (70 aa), FASTA scores: opt: 341,E(): 2.1e-16, (84.05% identity in 69 aa overlap). Similar to C-terminal part of various proteins e.g. Q9HPP8|ACC|VNG1532G biotin carboxylase from Halobacterium sp. strain NRC-1 (610 aa), FASTA scores: opt: 212, E(): 4e-07, (50.0% identity in 68 aa overlap); Q58628|PYCB_METJA|MJ1231 pyruvate carboxylase subunit B from Methanococcus jannaschii (567 aa), FASTA scores: opt: 192, E(): 7.8e-06, (44.8% identity in 58 aa overlap); Q9ZAA7|GCDC glutaconyl-CoA decarboxylase gamma subunit from Acidaminococcus fermentans (145 aa), FASTA scores: opt: 184, E(): 8.9e-06, (39.4% identity in 66 aa overlap); etc.; Biotinylated protein TB7.3 3597766 TB7.3 888096 TB7.3 Mycobacterium tuberculosis H37Rv Biotinylated protein TB7.3 NP_217737.1 3597551 R 83332 CDS YP_177945.1 57117075 3205091 complement(3598051..3598356) 1 NC_000962.3 Rv3221A, len: 101 aa. RshA, anti-sigma factor,similar to Q9XCD7|AAD41811.1 unknown protein from Mycobacterium smegmatis, linked to sigma factor sigH (see Fernandes et al., 1999) (101 aa), FASTA scores: opt: 422,E(): 3.4e-22, (64.9% identity in 94 aa overlap); and to Q9RL96|RsrA anti-sigma factor from Streptomyces coelicolor (see Kang et al., 1999) (105 aa), FASTA scores: opt: 163,E(): 0.00016, (32.05% identity in 78 aa overlap).; Anti-sigma factor RshA 3598356 rshA 3205091 rshA Mycobacterium tuberculosis H37Rv Anti-sigma factor RshA YP_177945.1 3598051 R 83332 CDS NP_217738.1 15610358 887655 complement(3598353..3598904) 1 NC_000962.3 Rv3222c, (MTCY07D11.04), len: 183 aa. Hypothetical protein, with some similarity to Q9SZD2|F19B15.50|AT4G29020 glycine-rich protein like from Arabidopsis thaliana (Mouse-ear cress) (158 aa), FASTA scores: opt: 131, E(): 0.77, (33.35% identity in 126 aa overlap); Q9S222|SCI51.18 putative transcriptional regulator from Streptomyces coelicolor (548 aa), FASTA scores: opt: 133, E(): 1.6,(36.25% identity in 149 aa overlap); etc. Also some similarity to other hypothetical Mycobacterium tuberculosis proteins e.g. O06292|Rv0341|MTCY13E10.01 (479 aa), FASTA scores: opt: 141, E(): 0.5, (31.2% identity in 170 aa overlap); AAK45760|MT1497.1 PE_PGRS family protein from strain CDC1551 (1408 aa), FASTA scores: opt: 137, E(): 2,(31.75% identity in 148 aa overlap); etc.; hypothetical protein 3598904 887655 Rv3222c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217738.1 3598353 R 83332 CDS NP_217739.1 15610359 888094 complement(3598901..3599551) 1 NC_000962.3 Rv3223c, (MTCY07D11.03), len: 216 aa. SigH (alternate gene name: rpoE), alternative RNA polymerase sigma factor (see citations below), similar to many e.g. Q9XCD8|sigh from Mycobacterium smegmatis (215 aa), FASTA scores: opt: 1187, E(): 8.1e-69, (87.75% identity in 212 aa overlap); O87834|SIGR from Streptomyces coelicolor (227 aa), FASTA scores: opt: 913, E(): 2.6e-51, (68.8% identity in 202 aa overlap); O68520|RPOE1 from Myxococcus xanthus (213 aa), FASTA scores: opt: 452, E(): 6.7e-22, (42.8% identity in 187 aa overlap); Q06198|RPSH_PSEAE|ALGU|ALGT|PA0762 from Pseudomonas aeruginosa (193 aa), FASTA scores: opt: 301, E(): 2.7e-12,(29.9% identity in 194 aa overlap); etc. Equivalent to AAK47662 RNA polymerase sigma-70 factor from Mycobacterium tuberculosis strain CDC1551 (284 aa), but shorter 68 aa. Has sigma-70 factors ECF subfamily signature (PS01063). So belongs to the sigma-70 factor family, ECF subfamily. Start chosen on basis of similarity, other potential starts upstream.; Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE) 3599551 sigH 888094 sigH Mycobacterium tuberculosis H37Rv Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE) NP_217739.1 3598901 R 83332 CDS NP_217740.1 15610360 888852 3599851..3600699 1 NC_000962.3 Rv3224, (MTCY07D11.02c), len: 282 aa. Probable iron-regulated oxidoreductase, possible short-chain dehydrogenase/reductase, highly similar to BAB49551|MLL2413 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (288 aa), FASTA scores: opt: 1053, E(): 6.4e-59,(57.95% identity in 276 aa overlap); Q9AB34|CC0400 short chain dehydrogenase family protein from Caulobacter crescentus (285 aa), FASTA scores: opt: 1051, E(): 8.5e-59,(55.9% identity in 281 aa overlap); and Q9VB10|CG5590 hypothetical protein (similar to the short-chain dehydrogenases/reductases (SDR) family) from Drosophila melanogaster (Fruit fly) (412 aa), FASTA scores: opt: 966,E(): 2.5e-53, (52.15% identity in 278 aa overlap). Similar to various proteins (principaly oxidoreductases) e.g. Q18639|C45B11.3 hypothetical protein (similar to the SDR family) from Caenorhabditis elegans (293 aa), FASTA scores: opt: 921, E(): 1.2e-50, (51.3% identity in 271 aa overlap); Q9HZV5|PA2892 probable short-chain dehydrogenase from Pseudomonas aeruginosa (274 aa), FASTA scores: opt: 847,E(): 5.1e-46, (49.25% identity in 274 aa overlap); Q9I6V0|PA0182 probable short-chain dehydrogenase (similar to the SDR family) from Pseudomonas aeruginosa (250 aa),FASTA scores: opt: 333, E(): 8.3e-14, (29.8% identity in 245 aa overlap); Q9HY98|PA3511 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa), FASTA scores: opt: 330, E(): 1.3e-13, (31.2% identity in 250 aa overlap); etc. Related proteins in Mycobacterium tuberculosis include MTCY02B10.14, MTCY369.14, and MTCY09F9.36. Has ATP/GTP-binding site motif A, (PS00017) near C-terminus. May be belong to the short-chain dehydrogenases/reductases (SDR) family.; Possible iron-regulated short-chain dehydrogenase/reductase 3600699 888852 Rv3224 Mycobacterium tuberculosis H37Rv Possible iron-regulated short-chain dehydrogenase/reductase NP_217740.1 3599851 D 83332 CDS YP_177946.1 57117076 3205092 3600635..3600823 1 NC_000962.3 Rv3224A, len: 62 aa. Conserved hypothetical protein (possibly gene fragment), overlaps Rv3224. Similar to N-terminus of ML0799|AL583919_131 conserved hypothetical protein from Mycobacterium leprae (135 aa), FASTA scores: opt: 104, E(): 0.78, (59.37% identity in 32 aa overlap). Note that upstream ORF Rv3224B is similar to C-terminus of ML0799. There appears to be no frameshift as sequence is identical in strain CDC1551 and in Mycobacterium bovis. Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 3600823 3205092 Rv3224A Mycobacterium tuberculosis H37Rv hypothetical protein YP_177946.1 3600635 D 83332 CDS YP_177947.1 57117077 3205093 3600801..3601019 1 NC_000962.3 Rv3224B, len: 72 aa. Conserved hypothetical protein (possibly gene fragment), similar to C-terminal part of ML0799|AL583919_131 conserved hypothetical protein from Mycobacterium leprae (135 aa), FASTA scores: opt: 229, E(): 2e-09, (60.00% identity in 70 aa overlap). Note that downstream ORF Rv3224A is similar to N-terminus of ML0799. There appears to be no frameshift as sequence is identical in strain CDC1551 and in Mycobacterium bovis. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 3601019 3205093 Rv3224B Mycobacterium tuberculosis H37Rv hypothetical protein YP_177947.1 3600801 D 83332 CDS NP_217742.1 15610361 888804 complement(3601016..3602440) 1 NC_000962.3 Rv3225c, (MTCY07D11.01), len: 474 aa. Conserved hypothetical protein has GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part (see Vetting et al. 2005) and phosphotransferase domain in C-terminal part. C-terminal part shows similarity to various bacterial phosphotransferases e.g. BAB49093|MLL1809 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (298 aa),FASTA scores: opt: 557, E(): 2.8e-26, (34.55% identity in 295 aa overlap); P14509|KKA8_ECOLI|APHA aminoglycoside 3'-phosphotransferase from Escherichia coli (271 aa), FASTA scores: opt: 194, E(): 0.00018, (27.75% identity in 227 aa overlap); Q53826|CPH capreomycin phosphotransferase from Streptomyces capreolus (281 aa), FASTA scores: opt: 178,E(): 0.0017, (30.5% identity in 269 aa overlap).; GCN5-related N-acetyltransferase, phosphorylase 3602440 888804 Rv3225c Mycobacterium tuberculosis H37Rv GCN5-related N-acetyltransferase, phosphorylase NP_217742.1 3601016 R 83332 CDS NP_217743.1 15610362 888220 complement(3602564..3603322) 1 NC_000962.3 Rv3226c, (MTCY20B11.01c), len: 252 aa. Conserved hypothetical protein, similar to various hypothetical bacterial proteins e.g. Q9CCI2|ML0793 putative bacteriophage protein from Mycobacterium leprae (252 aa),FASTA scores: opt: 1183, E(): 3.8e-68, (70.65% identity in 252 aa overlap); BAB54183|MLR7795 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (369 aa), FASTA scores: opt: 417, E(): 2.9e-19, (33.75% identity in 252 aa overlap); O64131 YOQW protein from Bacteriophage SPBc2 (224 aa), FASTA scores: opt: 413, E(): 3.4e-19, (38.5% identity in 244 aa overlap); O31916 YOQW protein from Bacillus subtilis (224 aa), FASTA scores: opt: 413, E(): 3.4e-19,(38.5% identity in 244 aa overlap); O34906 YOAM protein from Bacillus subtilis (227 aa), FASTA scores: opt: 401,E(): 2e-18, (37.7% identity in 244 aa overlap); Q9K4A5|SC7E4.11 hypothetical 30.8 KDA protein from Streptomyces coelicolor (271 aa), FASTA scores: opt: 383,E(): 3.3e-17, (39.6% identity in 283 aa overlap); etc.; hypothetical protein 3603322 888220 Rv3226c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217743.1 3602564 R 83332 CDS NP_217744.1 15610363 888753 3603377..3604729 1 NC_000962.3 Rv3227, (MTCY20B11.02), len: 450 aa. AroA,3-phosphoshikimate 1-carboxyvinyl transferase (see citation below), equivalent (but C-terminus longer) to Q9CCI3|AROA|ML0792 putative 3-phosphoshikimate 1-carboxyvinyl transferase from Mycobacterium leprae (430 aa), FASTA scores: opt: 1466, E(): 1.4e-78, (55.05% identity in 427 aa overlap). Contains PS00885 EPSP synthase signature 2. Belongs to the EPSP synthase family.; 3-phosphoshikimate 1-carboxyvinyltransferase AroA (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS) 3604729 aroA 888753 aroA Mycobacterium tuberculosis H37Rv 3-phosphoshikimate 1-carboxyvinyltransferase AroA (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS) NP_217744.1 3603377 D 83332 CDS NP_217745.1 15610364 888785 3604726..3605718 1 NC_000962.3 Rv3228, (MTCY20B11.03), len: 330 aa. Conserved hypothetical protein, equivalent to Q9CCI4|ML0791 hypothetical protein from Mycobacterium leprae (327 aa),FASTA scores: opt: 1828, E(): 1e-98, (84.0% identity in 331 aa overlap). Also similar to several hypothetical bacterial proteins e.g. Q9K4A8|SC7E4.08c from Streptomyces coelicolor (337 aa), FASTA scores: opt: 1051, E(): 1e-53, (52.65% identity in 338 aa overlap); Q9HUL3|PA4952 from Pseudomonas aeruginosa (339 aa), FASTA scores: opt: 392 ,E(): 1.4e-15,(34.85% identity in 281 aa overlap); Q9PFV1|XF0556 from Xylella fastidiosa (341 aa), FASTA scores: opt: 367, E(): 4e-14, (36.85% identity in 247 aa overlap); P45339|YJEQ_HAEIN|HI1714 from Haemophilus influenzae (346 aa), FASTA scores: opt: 355, E(): 2e-13, (31.65% identity in 281 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A.; hypothetical protein 3605718 888785 Rv3228 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217745.1 3604726 D 83332 CDS YP_177948.1 57117078 888821 complement(3605751..3607034) 1 NC_000962.3 Rv3229c, (MTCY20B11.04c), len: 427 aa. DesA3,linoleoyl-CoA desaturase, showing similarity with desaturases and other proteins e.g. Q08871|DES6|SLL0262 linoleoyl-CoA desaturase from Synechocystis sp. strain PCC 6803 (359 aa), FASTA scores: opt: 319, E(): 4e-13, (25.1% identity in 295 aa overlap); Q54795|DESD delta 6 desaturase from Spirulina platensis (368 aa), FASTA scores: opt: 268,E(): 7.7e-10, (25.0% identity in 300 aa overlap); Q9ZTU8|S276 protein with similarity to cytochrome B5 domain from Triticum aestivum (Wheat) (469 aa), FASTA scores: opt: 240, E(): 5.9e-08, (27.05% identity in 266 aa overlap); etc.; Possible linoleoyl-CoA desaturase (delta(6)-desaturase) 3607034 desA3 888821 desA3 Mycobacterium tuberculosis H37Rv Possible linoleoyl-CoA desaturase (delta(6)-desaturase) YP_177948.1 3605751 R 83332 CDS NP_217747.1 15610366 888748 complement(3607112..3608254) 1 NC_000962.3 Rv3230c, (MTCY20B11.05c), len: 380 aa. Putative oxidoreductase, with some similarity to various proteins,especially reductases e.g. Q9HUS4|PA4889 probable oxidoreductase from Pseudomonas aeruginosa (366 aa), FASTA scores: opt: 516, E(): 1.8e-24, (33.8% identity in 367 aa overlap); P95533|TDNB electron transfer protein from Pseudomonas putida (337 aa), FASTA scores: opt: 380, E(): 4e-16, (30.7% identity in 277 aa overlap); BAB34381|ECS0958 NADH oxidoreductase for the HCP from Escherichia coli strain O157:H7 (322 aa), FASTA scores: opt: 369, E(): 1.8e-15, (28.65% identity in 328 aa overlap); Q44253|ATDA5 aniline dioxygenase reductase component from Acinetobacter sp. (336 aa), FASTA scores: opt: 305, E(): 1.6e-11, (27.4% identity in 303 aa overlap); etc.; Hypothetical oxidoreductase 3608254 888748 Rv3230c Mycobacterium tuberculosis H37Rv Hypothetical oxidoreductase NP_217747.1 3607112 R 83332 CDS NP_217748.1 15610367 888749 complement(3608364..3608873) 1 NC_000962.3 Rv3231c, (MTCY20B11.06c), len: 169 aa. Conserved protein, similar to Q9KYX9|SCE33.03c hypothetical 17.4 KDA protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 415, E(): 6.6e-19, (49.1% identity in 171 aa overlap).; hypothetical protein 3608873 888749 Rv3231c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217748.1 3608364 R 83332 CDS NP_217749.1 15610368 888760 complement(3608870..3609757) 1 NC_000962.3 Rv3232c, (MTCY20B11.07c), len: 295 aa (start uncertain). Ppk2, polyphosphate kinase 2, highly similar to Q9I154|PA2428 hypothetical protein from Pseudomonas aeruginosa (304 aa), FASTA scores: opt: 1057, E(): 6.8e-62,(60.7% identity in 252 aa overlap); Q9I6Z1|PA0141 hypothetical protein from Pseudomonas aeruginosa (298 aa),FASTA scores: opt: 990, E(): 1.6e-57, (54.6% identity in 249 aa overlap); and other hypothetical bacterial proteins. Note that previously known as pvdS. Ppk2|Rv3232c and NdkA|Rv2445c interact (See Sureka et al., 2009).; Polyphosphate kinase Ppk2 (polyphosphoric acid kinase) 3609757 ppk2 888760 ppk2 Mycobacterium tuberculosis H37Rv Polyphosphate kinase Ppk2 (polyphosphoric acid kinase) NP_217749.1 3608870 R 83332 CDS NP_217750.1 15610369 888773 complement(3609781..3610371) 1 NC_000962.3 Rv3233c, (MTCY20B11.08c), len: 196 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 308, E(): 1.2e-12, (32.0% identity in 200 aa overlap); and several hypothetical M. tuberculosis proteins e.g. O06343|YY80_MYCTU|Rv3480c|MTCY13E12.33c (497 aa),FASTA scores: opt: 248, E(): 9.8e-09, (27.5% identity in 200 aa overlap); MTCY28_26; MTCY493_29; MTCY31_25; MTCY31_25.; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 3610371 888773 Rv3233c Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_217750.1 3609781 R 83332 CDS NP_217751.1 15610370 888767 complement(3610374..3611189) 1 NC_000962.3 Rv3234c, (MTCY20B11.09c), len: 271 aa. Putative tgs3, triacylglycerol synthase (See Daniel et al., 2004),similar to C-terminus of Mycobacterium tuberculosis hypothetical proteins e.g. P71694|Rv1425|MTCY21B4.43|MTCY493.29c (459 aa), FASTA scores: opt: 498, E(): 5.2e-24, (36.8% identity in 261 aa overlap); MTCY03A2.28; MTCY31.23; MTCY493_29; MTCY28_26; MTV013_8; MTY13E12_33; etc. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 309, E(): 4.3e-12, (33.35% identity in 189 aa overlap).; Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs3 3611189 tgs3 888767 tgs3 Mycobacterium tuberculosis H37Rv Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs3 NP_217751.1 3610374 R 83332 CDS NP_217752.1 15610371 888862 3611300..3611941 1 NC_000962.3 Rv3235, (MTCY20B11.10), len: 213 aa. Hypothetical unknown ala-, arg-, pro-rich protein.; Hypothetical alanine arginine proline rich protein 3611941 888862 Rv3235 Mycobacterium tuberculosis H37Rv Hypothetical alanine arginine proline rich protein NP_217752.1 3611300 D 83332 CDS YP_177949.1 57117079 888861 complement(3611959..3613116) 1 NC_000962.3 Rv3236c, (MTCY20B11.11c), len: 385 aa. Probable conserved integral membrane transport protein, possibly cation (Na/H) transporter, equivalent to Q9CCI5|ML0782 putative transmembrane transport protein from Mycobacterium leprae (385 aa), FASTA scores: opt: 1975, E(): 2.4e-108,(81.55% identity in 385 aa overlap). Highly similar to others e.g. O69958|SC4H2.03c putative transmembrane transport protein from Streptomyces coelicolor (411 aa),FASTA scores: opt: 1226, E(): 1.6e-64, (53.5% identity in 372 aa overlap); Q9XAKO|SC66T3.13c putative transmembrane transport protein from Streptomyces coelicolor (403 aa),FASTA scores: opt: 1198, E(): 6.8e-63, (53.25% identity in 370 aa overlap); Q9RV80|DR1149 putative Na+/H+ antiporter from Deinococcus radiodurans (383 aa), FASTA scores: opt: 1069, E(): 2.3e-55, (47.35% identity in 376 aa overlap); Q9L191|SC10G8.11 putative transmembrane transport protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 695, E(): 1.9e-33, (38.05% identity in 384 aa overlap); Q9RRW8|DR2367 putative glutathione-regulated potassium-efflux system protein KEFB from Deinococcus radiodurans (575 aa), FASTA scores: opt: 414, E(): 6.2e-17,(30.25% identity in 380 aa overlap); etc. Seems to belong to the CPA2 family. Note that previously known as kefB.; Probable conserved integral membrane transport protein 3613116 888861 Rv3236c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane transport protein YP_177949.1 3611959 R 83332 CDS NP_217754.1 15610373 888803 complement(3613121..3613603) 1 NC_000962.3 Rv3237c, (MTCY20B11.12c), len: 160 aa. Conserved protein, equivalent to Q9CCI6|ML0781 hypothetical protein from Mycobacterium leprae (160 aa), FASTA scores: opt: 828,E(): 1.5e-45, (80.6% identity in 160 aa overlap); and similar to other hypothetical bacterial proteins and more weakly to putative potassium channels e.g. Q9RV81|DR1148 conserved hypothetical protein from Deinococcus radiodurans (175 aa), FASTA scores: opt: 420, E(): 9.5e-20, (37.95% identity in 158 aa overlap); O69959|SC4H2.04c hypothetical 17.1 KDA protein from Streptomyces coelicolor (161 aa),FASTA scores: opt: 315, E(): 3.8e-13, (40.0% identity in 150 aa overlap); Q9HNH3|PCHB|VNG2104G potassium channel homolog from Halobacterium sp. strain NRC-1 (418 aa), FASTA scores: opt: 158, E(): 0.007, (31.45% identity in 124 aa overlap); Q58752|YD57_METJA|MJ1357 putative potassium channel protein from Methanococcus jannaschii (343 aa),FASTA scores: opt: 143, E(): 0.053, (33.8% identity in 68 aa overlap).; hypothetical protein 3613603 888803 Rv3237c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217754.1 3613121 R 83332 CDS NP_217755.1 15610374 888858 complement(3613664..3614398) 1 NC_000962.3 Rv3238c, (MTCY20B11.13c), len: 244 aa. Probable conserved integral membrane protein, similar to several hypothetical proteins and transmembrane proteins e.g. Q9UN92|NRM29 multispanning nuclear envelope membrane protein NURIM (fragment) from Homo sapiens (Human) (261 aa), FASTA scores: opt: 281, E(): 3.3e-11, (30.7% identity in 189 aa overlap); Q9VEG9|CG7655 hypothetical protein from Drosophila melanogaster (Fruit fly) (253 aa), FASTA scores: opt: 242, E(): 1.1e-08, (27.7% identity in 242 aa overlap); BAB48937|MLR1600 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (222 aa), FASTA scores: opt: 137, E(): 0.066, (28.1% identity in 185 aa overlap); BAB57936|SAV1774 aesenical pump membrane protein homolog from Staphylococcus aureus subsp. aureus Mu50 (430 aa), FASTA scores: opt: 125,E(): 0.68, (25.7% identity in 144 aa overlap); etc.; Probable conserved integral membrane protein 3614398 888858 Rv3238c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217755.1 3613664 R 83332 CDS NP_217756.1 15610375 888856 complement(3614457..3617603) 1 NC_000962.3 Rv3239c, (MTCY20B11.14c), len: 1048 aa. Probable conserved transmembrane protein, organised in two domains. Domain comprising first ~500 aa residues is similar to various antibiotic resistance and efflux proteins and contains sugar transport proteins signature 1 (PS00216); e.g. Q9RL22|SC5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 905, E(): 3.1e-41, (36.95% identity in 482 aa overlap); and O68912|FRNF putative antibiotic antiporter from Streptomyces roseofulvus (517 aa), FASTA scores: opt: 866,E(): 4.1e-39, (37.1% identity in 512 aa overlap). Second part, corresponding to last 550 aa residues, is very similar to Q50733|Rv2565|MTCY9C4.03c hypothetical 62.1 kDa protein from Mycobacterium tuberculosis (583 aa), FASTA scores: E(): 2.1e-28, (36.5% identity in 572 aa overlap). Also equivalent to Rv3728|MTV025.076 putative two-domain membrane protein (similar to sugar transporter family) from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 4328, E(): 0, (64.15% identity in 1046 aa overlap); and similar to other Mycobacterium tuberculosis proteins: MTCY3G12.01, E(): 6.3e-32; MTCY98.02c, E(): 6.3e-32; MTCY9C4.03c, E(): 1.5e-26; MTCY369.27c, E(): 2.5e-26. Equivalent to AAK47679 Drug transporter from Mycobacterium tuberculosis strain CDC1551 (1065 aa) but shorter 20 aa. Contains cyclic nucleotide-binding domain signature 2 (PS00889). Probably member of major facilitator superfamily (MFS).; Probable conserved transmembrane transport protein 3617603 888856 Rv3239c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane transport protein NP_217756.1 3614457 R 83332 CDS YP_177950.1 57117080 888860 complement(3617682..3620531) 1 NC_000962.3 Rv3240c, (MTCY20B11.15c), len: 949 aa. Probable secA1, preprotein translocase subunit, component of secretion apparatus (see citations below), highly similar to many e.g. P57996|SEA1_MYCLE from Mycobacterium leprae (940 aa), FASTA scores: opt: 5044, E(): 0, (87.5% identity in 849 aa overlap); P95759|SECA_STRGR from Streptomyces griseus (940 aa), FASTA scores: opt: 2612, E(): 1.9e-134,(61.35% identity in 960 aa overlap); P28366|SECA_BACSU|div+ from Bacillus subtilis (841 aa), FASTA scores: opt: 1776,E(): 4.9e-89, (48.05% identity in 837 aa overlap); etc. Belongs to the SecA family. Part of the prokaryotic protein translocation apparatus which comprise SECA, SECD|Rv2587c,SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Note that previously known as secA. Binds ATP.; Probable preprotein translocase SecA1 1 subunit 3620531 secA1 888860 secA1 Mycobacterium tuberculosis H37Rv Probable preprotein translocase SecA1 1 subunit YP_177950.1 3617682 R 83332 CDS NP_217758.3 448824803 888849 complement(3620610..3621254) 1 NC_000962.3 Rv3241c, (MTCY20B11.16c), len: 214 aa. Conserved protein, similar to many hypothetical proteins and to some putative ribosomal proteins e.g. Q9CCI7|ML0778 hypothetical protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 1234, E(): 1.3e-72, (89.3% identity in 206 aa overlap); Q9KYX2|SCE33.11c hypothetical 27.9 KDA protein from Streptomyces coelicolor (254 aa), FASTA scores: opt: 487, E(): 2.2e-24, (47.6% identity in 210 aa overlap); Q9FLV3 protein similar to ribosomal protein 30S subunit from Arabidopsis thaliana (Mouse-ear cress) (365 aa), FASTA scores: opt: 264, E(): 7e-10, (26.4% identity in 212 aa overlap); P19954|RR30_SPIOL|RPS22 plastid-specific 30S ribosomal protein 1, chloroplast, from Spinacia oleracea (Spinach) (302 aa), FASTA scores: opt: 261, E(): 9.3e-10,(26.15% identity in 214 aa overlap); P47995|YSEA_STACA hypothetical protein in SECA 5'region (ORF1) (fragment) (belongs to the S30AE family of ribosomal proteins) from Staphylococcus carnosus (165 aa), FASTA scores: opt: 201,E(): 4.2e-06, (33.35% identity in 147 aa overlap); etc.; hypothetical protein 3621254 888849 Rv3241c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217758.3 3620610 R 83332 CDS NP_217759.1 15610378 888757 complement(3621570..3622211) 1 NC_000962.3 Rv3242c, (MTCY20B11.17c), len: 213 aa. Conserved hypothetical protein, highly similar in N-terminus to Q9CCI9|ML0776 hypothetical protein from Mycobacterium leprae (85 aa), FASTA scores: opt: 324, E(): 1.7e-13,(78.1% identity in 64 aa overlap). Also similar to Q9RUJ7|DR1389 putative competence protein COMF from Deinococcus radiodurans (219 aa), FASTA scores: opt: 223,E(): 6.3e-07, (35.8% identity in 215 aa overlap); BAB50338|MLL3453 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (240 aa), FASTA scores: opt: 218, E(): 1.4e-06, (28.5% identity in 224 aa overlap); Q9A9Y1|CC0830 competence protein F from Caulobacter crescentus (265 aa),FASTA scores: opt: 182, E(): 0.00026, (30.15% identity in 219 aa overlap); etc. Equivalent to AAK47682 from Mycobacterium tuberculosis strain CDC1551 (241 aa) but shorter 29 aa. Contains purine/pyrimidine phosphoribosyl transferases signature (PS00103). Seems to belong to purine/pyrimidine phosphoribosyl transferase family.; hypothetical protein 3622211 888757 Rv3242c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217759.1 3621570 R 83332 CDS NP_217760.1 15610379 888810 complement(3622249..3623091) 1 NC_000962.3 Rv3243c, (MTCY20B11.18c), len: 280 aa. Unknown protein.; hypothetical protein 3623091 888810 Rv3243c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217760.1 3622249 R 83332 CDS NP_217761.1 15610380 888815 complement(3623159..3624910) 1 NC_000962.3 Rv3244c, (MTCY20B11.19c), len: 583 aa. Probable lpqB, conserved lipoprotein; contains appropriately placed lipoprotein signature (PS00013). Equivalent to Q9CCJ0|LPQB|ML0775 putative lipoprotein from Mycobacterium leprae (589 aa), FASTA scores: opt: 3375, E(): 1.4e-186,(87.9% identity in 579 aa overlap). Also similar to various proteins (in particular transferases) e.g. Q9KYX0|SCE33.13c putative lipoprotein from Streptomyces coelicolor (615 aa),FASTA scores: opt: 228, E(): 1.3e-05, (25.5% identity in 624 aa overlap); O87992|BBLPS1.19c putative glutamine amidotransferase from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (628 aa), FASTA scores: opt: 162, E(): 0.079, (28.05% identity in 171 aa overlap); Q9L2F4|SC7A8.01 putative sugar kinase (fragment) from Streptomyces coelicolor (434 aa), FASTA scores: opt: 143,E(): 0.72, (27.65% identity in 293 aa overlap); etc.; Probable conserved lipoprotein LpqB 3624910 lpqB 888815 lpqB Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqB NP_217761.1 3623159 R 83332 CDS NP_217762.1 15610381 888719 complement(3624910..3626613) 1 NC_000962.3 Rv3245c, (MTCY20B11.20c), len: 567 aa. MtrB,sensor-like histidine kinase (see citations below),equivalent to Q9CCJ1|MTRB or ML0774 putative two-component system sensor kinase from Mycobacterium leprae (562 aa),FASTA scores: opt: 3208, E(): 7.4e-173, (88.7% identity in 566 aa overlap). Also similar to others e.g. Q9KYW9|SCE33.14c putative two-component system histidine kinase from Streptomyces coelicolor (688 aa), FASTA scores: opt: 1355, E(): 1.1e-68, (48.95% identity in 515 aa overlap); etc. Relatives in Mycobacterium tuberculosis are: MTCY369.03, E(): 1.5e-22; MTCY20G9.16, E(): 1.9e-17. Similar to other prokaryotic sensory transduction histidine kinases.; Two component sensory transduction histidine kinase MtrB 3626613 mtrB 888719 mtrB Mycobacterium tuberculosis H37Rv Two component sensory transduction histidine kinase MtrB NP_217762.1 3624910 R 83332 CDS NP_217763.1 15610382 888743 complement(3626663..3627349) 1 NC_000962.3 Rv3246c, (MTCY20B11.21c), len: 228 aa. MtrA,transcriptional activator, response regulator (see citations below), equivalent to Q9CCJ2|MTRA|ML0773 putative two-component response regulator from Mycobacterium leprae (228 aa), FASTA scores: opt: 1458, E(): 1.4e-85, (98.7% identity in 228 aa overlap). Also highly similar to others e.g. Q9F9J5|SCRA putative response regulator from Streptomyces coelicolor (228 aa), FASTA scores: opt: 1141,E(): 1.9e-65, (74.9% identity in 227 aa overlap); Q9KYW8|SCE33.15c putative two-component system response regulator from Streptomyces coelicolor (229 aa), FASTA scores: opt: 1141, E(): 1.9e-65, (74.9% identity in 227 aa overlap); Q9F868|REGX3 response regulator REGX3 from Mycobacterium smegmatis (228 aa), FASTA scores: opt: 730,E(): 2.3e-39, (50.90% identity in 222 aa overlap); etc. Relatives in Mycobacterium tuberculosis are: U01971|MTU01971_1; Q11156|RGX3_MYCTU; MTCY20G9.17, E(): 0; MTCY31.31c, E(): 3.4e-29; MTCY369.02, E(): 5.7e-28. Similar to bacterial regulatory proteins involved in signal transduction. The N-terminal region is similar to that of other regulatory components of sensory transduction systems. Experiments showed mtrA is differentially expressed in virulent and avirulent strains during growth in macrophages.; Two component sensory transduction transcriptional regulatory protein MtrA 3627349 mtrA 888743 mtrA Mycobacterium tuberculosis H37Rv Two component sensory transduction transcriptional regulatory protein MtrA NP_217763.1 3626663 R 83332 CDS NP_217764.1 15610383 888740 complement(3627419..3628063) 1 NC_000962.3 Rv3247c, (MTCY20B11.22c), len: 214 aa. tmk,thymidylate kinase, equivalent to Q9CCJ3|TMK|ML0772 putative thymidylate kinase from Mycobacterium leprae (210 aa), FASTA scores: opt: 1023, E(): 4.8e-57, (77.3% identity in 207 aa overlap). Also similar to other thymidylate kinases e.g. Q9RQJ9|KTHY_CAUCR|TMK|CC1824 from Caulobacter crescentus (208 aa), FASTA scores: opt: 179, E(): 0.0003,(31.3% identity in 214 aa overlap); Q9V1E9|KTHY_PYRAB|TMK|PAB0319 from Pyrococcus abyssi (205 aa), FASTA scores: opt: 176, E(): 0.00045, (29.1% identity in 189 aa overlap); etc. Belongs to the thymidylate kinase family.; Thymidylate kinase Tmk (dTMP kinase) (thymidylic acid kinase) (TMPK) 3628063 tmk 888740 tmk Mycobacterium tuberculosis H37Rv Thymidylate kinase Tmk (dTMP kinase) (thymidylic acid kinase) (TMPK) NP_217764.1 3627419 R 83332 CDS NP_217765.1 15610384 888746 complement(3628160..3629647) 1 NC_000962.3 Rv3248c, (MTCY20B11.23c), len: 495 aa. Probable sahH, adenosylhomocysteinase, equivalent to Q9CCJ4|SAHH|ML0771 putative S-adenosyl-L-homocysteine hydrolase from Mycobacterium leprae (492 aa), FASTA scores: opt: 3019, E(): 1.3e-177, (91.4% identity in 489 aa overlap). Also highly similar to other adenosylhomocysteinases e.g. Q9KZM1|SAHH from Streptomyces coelicolor (485 aa), FASTA scores: opt: 2258, E(): 5.7e-131, (70.0% identity in 483 aa overlap); P51540|SAHH_TRIVA from Trichomonas vaginalis (486 aa),FASTA scores: opt: 2005, E(): 1.8e-115, (62.05% identity in 477 aa overlap); P35007|SAHH_CATRO from Catharanthus roseus (Rosy periwinkle) (Madagascar periwinkle) (485 aa), FASTA scores: opt: 1941, E(): 1.5e-111, (60.15% identity in 492 aa overlap); etc. Has S-adenosyl-L-homocysteine hydrolase signature (PS00739). Belongs to the adenosylhomocysteinase family.; Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) 3629647 sahH 888746 sahH Mycobacterium tuberculosis H37Rv Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) NP_217765.1 3628160 R 83332 CDS NP_217766.1 15610385 888741 complement(3629752..3630387) 1 NC_000962.3 Rv3249c, (MTCY20B11.24c), len: 211 aa. Possible transcriptional regulatory protein, TetR family, with similarity to several e.g. Q9AE61|ALKB1 putative TetR-regulatory from Rhodococcus erythropolis (208 aa),FASTA scores: opt: 503, E(): 7.7e-26, (40.6% identity in 192 aa overlap); CAC37620 putative TetR-regulatory protein from Prauserella rugosa (212 aa), FASTA scores: opt: 246,E(): 4.4e-09, (27.95% identity in 186 aa overlap); Q9K4B0|SC7E4.06 putative TetR-family transcriptional from Streptomyces coelicolor (203 aa), FASTA scores: opt: 224,E(): 1.1e-07, (34.5% identity in 197 aa overlap); Q11063|YC55_MYCTU|Rv1255c|MT1294|MTCY50.27 hypothetical transcriptional regulator from Mycobacterium tuberculosis (202 aa), FASTA scores: opt: 191, E(): 1.6e-05, (28.35% identity in 180 aa overlap); etc. Equivalent to AAK47689 from Mycobacterium tuberculosis strain CDC1551 (230 aa) but shorter 19 aa. Could belong to the TetR/AcrR family of transcriptional regulators. Possible helix-turn helix motif at aa 44-65 (+6.66 SD).; Possible transcriptional regulatory protein (probably TetR-family) 3630387 888741 Rv3249c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein (probably TetR-family) NP_217766.1 3629752 R 83332 CDS NP_217767.1 15610386 888744 complement(3630384..3630566) 1 NC_000962.3 Rv3250c, (MTCY20B11.25c), len: 60 aa. Probable rubB,rubredoxin, highly similar to other rubredoxins e.g. Q9AE66|RUBA4 from Rhodococcus erythropolis (60 aa), FASTA scores: opt: 391, E(): 2.2e-21, (83.05% identity in 59 aa overlap); Q9AE63|RUBA2 from Rhodococcus erythropolis (63 aa), FASTA scores: opt: 380, E(): 1.4e-20, (83.9% identity in 56 aa overlap); P42453|RUBR_ACICA|RUBA from Acinetobacter calcoaceticus (54 aa), FASTA scores: opt: 315, E(): 4.9e-16, (69.8% identity in 53 aa overlap); Q9HTK7|PA5351 from Pseudomonas aeruginosa (55 aa), FASTA scores: opt: 298, E(): 8e-15, (64.15% identity in 53 aa overlap); Q9PGC3|XF0379 from Xylella fastidiosa (57 aa),FASTA scores: opt: 263, E(): 2.5e-12, (59.25% identity in 54 aa overlap); etc. Also similar to neighbouring ORF M. tuberculosis RubA (MTCY20B11.26c). Contains rubredoxin signature (PS00202). Belongs to the rubredoxin family.; Probable rubredoxin RubB 3630566 rubB 888744 rubB Mycobacterium tuberculosis H37Rv Probable rubredoxin RubB NP_217767.1 3630384 R 83332 CDS NP_217768.1 15610387 888747 complement(3630571..3630738) 1 NC_000962.3 Rv3251c, (MTCY20B11.26c), len: 55 aa. Probable rubA,rubredoxin, highly similar to other rubredoxins (but sometimes shorter) e.g. Q9AE67|RUBA3 from Rhodococcus erythropolis (61 aa), FASTA scores: opt: 335, E(): 1e-17,(73.6% identity in 53 aa overlap); P00272|RUB2_PSEOL|ALKG from Pseudomonas oleovorans (172 aa), FASTA scores: opt: 278, E(): 2.7e-13, (65.3% identity in 49 aa overlap); CAC38028|ALKG from Alcanivorax borkumensis (174 aa), FASTA scores: opt: 271, E(): 8.6e-13, (62.0% identity in 50 aa overlap); Q9WWW4|ALKG from Pseudomonas putida (175 aa),FASTA scores: opt: 270, E(): 1e-12, (61.8% identity in 55 aa overlap); etc. Also highly similar to C-terminus of Q9XBM1|ALKB alkane 1-monooxygenase from Prauserella rugosa (490 aa), FASTA scores: opt: 296, E(): 2.9e-14, (75.5% identity in 49 aa overlap). Also similar to neighbouring ORF Mycobacterium tuberculosis rubB (MTCY20B11.25c). Contains rubredoxin signature (PS00202). Belongs to the rubredoxin family.; Probable rubredoxin RubA 3630738 rubA 888747 rubA Mycobacterium tuberculosis H37Rv Probable rubredoxin RubA NP_217768.1 3630571 R 83332 CDS NP_217769.1 15610388 888690 complement(3630738..3631988) 1 NC_000962.3 Rv3252c, (MTCY20B11.27c), len: 416 aa. Probable alkB, transmembrane alkane-1-monooxygenase, highly similar to many (see Marin et al., 2001) e.g. Q9AE68|ALKB2 from Rhodococcus erythropolis (408 aa), FASTA scores: opt: 2018,E(): 9.6e-122, (68.6% identity in 415 aa overlap); Q9AFD5|ALKB from Nocardioides sp. CF8 (483 aa), FASTA scores: opt: 1485, E(): 1.4e-87, (56.55% identity in 405 aa overlap); Q9XAU0|ALKB1 from Rhodococcus erythropolis (391 aa), FASTA scores: opt: 1400, E(): 3.3e-82, (62.6% identity in 396 aa overlap); Q9XBM1|ALKB from Prauserella rugosa (490 aa), FASTA scores: opt: 1266, E(): 1.5e-73, (57.55% identity in 410 aa overlap); CAC40954|ALKB4 from Rhodococcus erythropolis (386 aa), FASTA scores: opt: 1190,E(): 9.1e-69, (54.3% identity in 383 aa overlap); etc.; Probable transmembrane alkane 1-monooxygenase AlkB (alkane 1-hydroxylase) (lauric acid omega-hydroxylase) (omega-hydroxylase) (fatty acid omega-hydroxylase) (alkane hydroxylase-rubredoxin) 3631988 alkB 888690 alkB Mycobacterium tuberculosis H37Rv Probable transmembrane alkane 1-monooxygenase AlkB (alkane 1-hydroxylase) (lauric acid omega-hydroxylase) (omega-hydroxylase) (fatty acid omega-hydroxylase) (alkane hydroxylase-rubredoxin) NP_217769.1 3630738 R 83332 CDS NP_217770.1 15610389 888739 complement(3632097..3633584) 1 NC_000962.3 Rv3253c, (MTCY20B11.28c), len: 495 aa. Possible cationic amino acid transporter, integral membrane protein,similar to many e.g. O69844|SC1C3.02 putative cationic amino acid transporter from Streptomyces coelicolor (503 aa), FASTA scores: opt: 1649, E(): 5.8e-92, (52.6% identity in 485 aa overlap); Q9AE69 putative transporter (fragment) from Rhodococcus erythropolis (385 aa), FASTA scores: opt: 1594, E(): 9.7e-89, (62.0% identity in 387 aa overlap); Q9PBD7|XF2207 cationic amino acid transporter from Xylella fastidiosa (483 aa), FASTA scores: opt: 1079, E(): 1.2e-57,(40.55% identity in 493 aa overlap); Q9SRU9|F20H23.25 putative cationic amino acid transporter from Arabidopsis thaliana (Mouse-ear cress) (614 aa), FASTA scores: opt: 802, E(): 6.7e-41, (36.4% identity in 445 aa overlap); P30823|CTR1_RAT|SLC7A1|ATRC1 high-affinity cationic amino acid transporter-1 from Rattus norvegicus (Rat) (624 aa),FASTA scores: opt: 782, E(): 1.1e-39, (36.1% identity in 432 aa overlap); etc. Relatives in Mycobacterium tuberculosis include: MTCY3G12.14, E(): 5.6e-31; MTCY39.19,E(): 1.6e-14. Seems to belong to the APC family.; Possible cationic amino acid transport integral membrane protein 3633584 888739 Rv3253c Mycobacterium tuberculosis H37Rv Possible cationic amino acid transport integral membrane protein NP_217770.1 3632097 R 83332 CDS NP_217771.1 15610390 887237 3633675..3635063 1 NC_000962.3 Rv3254, (MTCY20B11.29), len: 462 aa. Conserved hypothetical protein, similar to CAC37877|SC1G7.02 putative secreted protein from Streptomyces coelicolor (440 aa),FASTA scores: opt: 606, E(): 6.2e-31, (31.7% identity in 445 aa overlap); O86550|SC1F2.13c hypothetical 50.7 KDA protein from Streptomyces coelicolor (476 aa), FASTA scores: opt: 577, E(): 4.5e-29, (32.5% identity in 400 aa overlap); Q9L0A8|SCC24.09 putative secreted protein from Streptomyces coelicolor (468 aa), FASTA scores: opt: 380,E(): 1.3e-16, (30.7% identity in 391 aa overlap); BAB48792|MLL1411 probable FAD-dependent monooxygenase from Rhizobium loti (Mesorhizobium loti) (421 aa), FASTA scores: opt: 128, E(): 1.1, (25.2% identity in 397 aa overlap); Q9L7X9|BENF benzoate-specific porin-like protein from Pseudomonas putida (397 aa), FASTA scores: opt: 119, E(): 4, (24.85% identity in 157 aa overlap); etc. Also similar to N-terminus of AAK46259|MT1987 putative ferredoxin reductase, electron transfer component from Mycobacterium tuberculosis strain CDC1551 (839 aa), FASTA scores: opt: 493, E(): 1.5e-23, (30.65% identity in 382 aa overlap).; hypothetical protein 3635063 887237 Rv3254 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217771.1 3633675 D 83332 CDS NP_217772.1 15610391 888698 complement(3635041..3636267) 1 NC_000962.3 Rv3255c, (MTCY20B11.30c), len: 408 aa. Probable manA, mannose-6-phosphate isomerase, equivalent to Q9CCJ5|MANA|ML0765 putative mannose-6-phosphate isomerase from Mycobacterium leprae (410 aa), FASTA scores: opt: 2271, E(): 1.6e-133, (84.45% identity in 411 aa overlap). Also similar to many others e.g. Q9KZL9|MANA from Streptomyces coelicolor (383 aa), FASTA scores: opt: 946,E(): 2.4e-51, (44.4% identity in 403 aa overlap); Q9KV87|VC0269 from Vibrio cholerae (399 aa), FASTA scores: opt: 726, E(): 1.1e-37, (34.15% identity in 404 aa overlap); Q9CMJ5|PMI|PM0829 from Pasteurella multocida (400 aa), FASTA scores: opt: 640, E(): 2.4e-32, (32.5% identity in 391 aa overlap); etc. Similar to family 1 of mannose-6-phosphate isomerases.; Probable mannose-6-phosphate isomerase ManA (phosphomannose isomerase) (phosphomannoisomerase) (PMI) (phosphohexoisomerase) (phosphohexomutase) 3636267 manA 888698 manA Mycobacterium tuberculosis H37Rv Probable mannose-6-phosphate isomerase ManA (phosphomannose isomerase) (phosphomannoisomerase) (PMI) (phosphohexoisomerase) (phosphohexomutase) NP_217772.1 3635041 R 83332 CDS NP_217773.1 15610392 888696 complement(3636275..3637315) 1 NC_000962.3 Rv3256c, (MTV015.01c-MTCY20B11.31c), len: 346 aa. Conserved protein, equivalent to Q9CCJ6|ML0764 hypothetical protein from Mycobacterium leprae (365 aa), FASTA scores: opt: 1574, E(): 1.4e-82, (75.35% identity in 365 aa overlap). Also similar to other hypothetical bacterial proteins e.g. Q9KZL8|SCE34.07c from Streptomyces coelicolor (375 aa), FASTA scores: opt: 171, E(): 0.012, (31.1% identity in 376 aa overlap); P55709|Y4YA_RHISN from Rhizobium sp. strain NGR234 (457 aa), FASTA scores: opt: 140, E(): 0.84, (28.75% identity in 233 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3637315 888696 Rv3256c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217773.1 3636275 R 83332 CDS NP_217774.1 15610393 888699 complement(3637312..3638709) 1 NC_000962.3 Rv3257c, (MTV015.02c), len: 465 aa. Probable pmmA,phosphomannomutase, equivalent to Q9CCJ7|PMMA|ML0763 phosphomannomutase from Mycobacterium leprae (468 aa),FASTA scores: opt: 2533, E(): 2e-145, (83.1% identity in 468 aa overlap). Also similar to many e.g. Q9KZL6|MANB from Streptomyces coelicolor (454 aa), FASTA scores: opt: 1820,E(): 2e-102, (63.2% identity in 459 aa overlap); Q9PGN8|XF0260 from Xylella fastidiosa (500 aa), FASTA scores: opt: 1085, E(): 4.7e-58, (40.7% identity in 462 aa overlap); Q9EY19|MANB from Salmonella enterica subsp. arizonae (456 aa), FASTA scores: opt: 988, E(): 3.1e-52,(38.65% identity in 445 aa overlap); etc. Belongs to the phosphohexose mutases family.; Probable phosphomannomutase PmmA (PMM) (phosphomannose mutase) 3638709 pmmA 888699 pmmA Mycobacterium tuberculosis H37Rv Probable phosphomannomutase PmmA (PMM) (phosphomannose mutase) NP_217774.1 3637312 R 83332 CDS NP_217775.1 15610394 888694 complement(3638811..3639302) 1 NC_000962.3 Rv3258c, (MTV015.03c), len: 163 aa. Conserved hypothetical protein, equivalent to Q9CCJ8|ML0762 hypothetical protein from Mycobacterium leprae (165 aa),FASTA scores: opt: 840, E(): 9.9e-42, (76.9% identity in 169 aa overlap). Also similar to Q9KZL4|SCE34.11c hypothetical 15.0 KDA protein from Streptomyces coelicolor (140 aa), FASTA scores: opt: 353, E(): 1.1e-13, (48.3% identity in 147 aa overlap); and shows really weak similarity to other bacterial proteins.; hypothetical protein 3639302 888694 Rv3258c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217775.1 3638811 R 83332 CDS NP_217776.1 15610395 888697 3639425..3639844 1 NC_000962.3 Rv3259, (MTV015.04), len: 139 aa. Conserved hypothetical protein, equivalent, but shorter 29 aa, to Q9CCJ9|ML0761 hypothetical protein from Mycobacterium leprae (167 aa), FASTA scores: opt: 846, E(): 2.2e-47,(89.2% identity in 139 aa overlap). C-terminus highly similar to Q9S425 hypothetical 6.0 KDA protein (fragment) from Mycobacterium smegmatis (54 aa), FASTA scores: opt: 275, E(): 2.7e-11, (81.15% identity in 53 aa overlap). Also similar to Q9KZL3|SCE34.12 from Streptomyces coelicolor (117 aa), FASTA scores: opt: 152, E(): 0.004, (34.15% identity in 126 aa overlap). Equivalent to AAK47699 from Mycobacterium tuberculosis strain CDC1551 (175 aa) but shorter 36 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3639844 888697 Rv3259 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217776.1 3639425 D 83332 CDS NP_217777.1 15610396 888687 complement(3639872..3640141) 1 NC_000962.3 Rv3260c, (MTV015.05c), len: 89 aa. Probable whiB2 (alternate gene name: whmD), WhiB-like regulatory protein (see Hutter & Dick 1999), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q9CCK0|WHIB2|ML0760 putative transcriptional regulator from Mycobacterium leprae (89 aa), FASTA scores: opt: 550, E(): 6.1e-31, (85.4% identity in 89 aa overlap). Also similar to others e.g. Q9S426 WHMD regulatory protein (see Gomez & Bishai 2000) from Mycobacterium smegmatis (129 aa), FASTA scores: opt: 488, E(): 1.4e-26, (83.55% identity in 85 aa overlap); Q06387|WHIB-STV WHIB-STV protein from Streptomyces griseocarneus (87 aa), FASTA scores: opt: 443,E(): 1.2e-23, (74.7% identity in 83 aa overlap); Q05429|WHIB|WHIB1 transcription-like factor WhiB from Streptomyces aureofaciens (87 aa), FASTA scores: opt: 442,E(): 1.3e-23, (74.7% identity in 83 aa overlap); etc. Equivalent to AAK47700 WhiB-related protein from Mycobacterium tuberculosis strain CDC1551 (123 aa) but shorter 34 aa. Also similar to other Mycobacterium tuberculosis proteins: MTCY07D11.07c (45.1% identity in 71 aa overlap) and MTCY78.13c (37.4% identity in 91 aa overlap). Start chosen by homology but ORF continues to ATG upstream at 3754.; Probable transcriptional regulatory protein WhiB-like WhiB2 3640141 whiB2 888687 whiB2 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein WhiB-like WhiB2 NP_217777.1 3639872 R 83332 CDS NP_217778.1 15610397 888701 3640543..3641538 1 NC_000962.3 Rv3261, (MTCY71.01), len: 331 aa. Probable fbiA,F420 biosynthesis protein, equivalent to FBIA F420 biosynthesis protein fbiA from Mycobacterium bovis BCG (see citations below). Also equivalent, but shorter 46 aa, to Q9CCK1|ML0759 hypothetical protein from Mycobacterium leprae (379 aa), FASTA scores: opt: 1855, E(): 3.9e-110,(79.3% identity in 333 aa overlap). Also similar to others e.g. Q9KZK9|SCE34.17 hypothetical 33.6 KDA protein from Streptomyces coelicolor (319 aa), FASTA scores: opt: 1151,E(): 1.2e-65, (55.1% identity in 332 aa overlap); O29345|AF0917 conserved hypothetical protein from Archaeoglobus fulgidus (296 aa), FASTA scores: opt: 469,E(): 1.7e-22, (31.15% identity in 302 aa overlap); Q58653|MJ1256 hypothetical protein from Methanococcus jannaschii (311 aa), FASTA scores: opt: 436, E(): 2.2e-20,(27.35% identity in 274 aa overlap); etc.; Probable F420 biosynthesis protein FbiA 3641538 fbiA 888701 fbiA Mycobacterium tuberculosis H37Rv Probable F420 biosynthesis protein FbiA NP_217778.1 3640543 D 83332 CDS NP_217779.1 15610398 888693 3641535..3642881 1 NC_000962.3 Rv3262, (MTCY71.02), len: 448 aa. Probable fbiB,F420 biosynthesis protein, equivalent to FBIB F420 biosynthesis protein fbiB from Mycobacterium bovis BCG (see citations below). Also equivalent to Q9CCK2|ML0758 putative oxidoreductase from Mycobacterium leprae (457 aa), FASTA scores: opt: 2411, E(): 3.5e-137, (82.25% identity in 445 aa overlap). Also similar to Q9KZK8|SCE34.18 putative oxidoreductase from Streptomyces coelicolor (443 aa), FASTA scores: opt: 1180, E(): 2.2e-63, (51.75% identity in 433 aa overlap); other oxidoreductases in C-terminus; and several hypothetical bacterial proteins.; Probable F420 biosynthesis protein FbiB 3642881 fbiB 888693 fbiB Mycobacterium tuberculosis H37Rv Probable F420 biosynthesis protein FbiB NP_217779.1 3641535 D 83332 CDS NP_217780.1 15610399 888691 3643177..3644838 1 NC_000962.3 Rv3263, (MTCY71.03), len: 553 aa. Probable DNA methylase, equivalent to Q9CCK4|ML0756 probable DNA methylase from Mycobacterium leprae (555 aa), FASTA scores: opt: 2980, E(): 2.1e-184, (81.9% identity in 541 aa overlap). Also similar to others e.g. P25240|MT57_ECOLI|ECO57IM modification methylase from Escherichia coli (544 aa), FASTA scores: opt: 595, E(): 1e-30, (30.35% identity in 507 aa overlap); P25201|MTA1_ACICA|ACCIM modification methylase ACCI from Acinetobacter calcoaceticus (540 aa), FASTA scores: opt: 366, E(): 5.7e-16, (23.35% identity in 467 aa overlap); Q56752|M-ACCI ACCI methylase from Bergeyella zoohelcum (541 aa), FASTA scores: opt: 365, E(): 6.6e-16, (22.95% identity in 466 aa overlap); etc. Contains PS00092 N-6 Adenine-specific DNA methylases signature. Alternative start site at aa 25.; Probable DNA methylase (modification methylase) (methyltransferase) 3644838 888691 Rv3263 Mycobacterium tuberculosis H37Rv Probable DNA methylase (modification methylase) (methyltransferase) NP_217780.1 3643177 D 83332 CDS YP_177951.1 57117081 888715 complement(3644898..3645977) 1 NC_000962.3 Rv3264c, (MTCY71.04c), len: 359 aa. ManB (alternate gene name: hddC), D-alpha-D-mannose-1-phosphate guanylyltransferase (see citations below), equivalent to Q9CCK6|RMLA2|ML0753 putative sugar-phosphate nucleotidyl transferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2075, E(): 2.7e-115, (86.9% identity in 359 aa overlap). Also similar to others e.g. Q9KZK6|SCE34.20c putative nucleotide phosphorylase from Streptomyces coelicolor (360 aa), FASTA scores: opt: 1314, E(): 2.2e-70,(57.0% identity in 358 aa overlap); Q9KZP4|SC1A8A.08 putative mannose-1-phosphate guanyltransferase from Streptomyces coelicolor (831 aa), FASTA scores: opt: 699,E(): 8.6e-34, (34.45% identity in 354 aa overlap) (only similarity in N-terminus for this one); P74589|SLL1496 mannose-1-phosphate guanyltransferase from Synechocystis sp. strain PCC 6803 (843 aa), FASTA scores: opt: 692, E(): 2.3e-33, (35.1% identity in 342 aa overlap) (only similarity in N-terminus for this one too); BAB59222|TVG0079558 mannose-1-phosphate guanyltransferase from Thermoplasma volcanium (359 aa), FASTA scores: opt: 664, E(): 5.2e-32, (34.6% identity in 338 aa overlap); Q9ZTW5|GMP GDP-mannose pyrophosphorylase from Solanum tuberosum (Potato) (361 aa), FASTA scores: opt: 636, E(): 2.3e-30, (34.65% identity in 361 aa overlap); etc. Belongs to family 2 of mannose-6-phosphate isomerases. Note that previously known as rmlA2.; D-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-alpha-D-heptose-1-phosphate guanylyltransferase) 3645977 manB 888715 manB Mycobacterium tuberculosis H37Rv D-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-alpha-D-heptose-1-phosphate guanylyltransferase) YP_177951.1 3644898 R 83332 CDS YP_177952.3 448824804 888714 complement(3645979..3646884) 1 NC_000962.3 Rv3265c, (MTCY71.05c), len: 301 aa. wbbL1,dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol a-3-L-rhamnosyl transferase (see citations below), equivalent to Q9CCK7|WBBL|ML0752 putative dTDP-rhamnosyl transferase from Mycobacterium leprae (308 aa), FASTA scores: opt: 1788,E(): 3e-104, (85.05% identity in 301 aa overlap); and Q9RN50|WBBL|Q9RN49 (see note * below) dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol,a-3-L-rhamnosyl transferase from Mycobacterium smegmatis (296 aa), FASTA scores: opt: 1494, E(): 6.1e-86, (72.35% identity in 293 aa overlap). Note that previously known as wbbL. [* Note: unpublished (experimental study on Mycobacterium smegmatis). Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases - The cell wall arabinogalactan linker formation enzyme, dTDP-Rha:a-D-GlcNAc-diphosphoryl polyprenol, a-3-L-rhamnosyl transferase is essential for mycobacterial viability - Mills J.A., Motichka K., Jucker M., Wu H.P., Uhlic B.C., Stern R.J., Scherman M.S., Vissa V.D., Yan W., Pan F., Kimbrel S., Kundu M., McNeil M.].; dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol,a-3-L-rhamnosyl transferase WbbL1 (alpha-L-rhamnose-(1->3)-alpha-D-GlcNAc(1->P)-P- decaprenyl) 3646884 wbbL1 888714 wbbL1 Mycobacterium tuberculosis H37Rv dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol,a-3-L-rhamnosyl transferase WbbL1 (alpha-L-rhamnose-(1->3)-alpha-D-GlcNAc(1->P)-P- decaprenyl) YP_177952.3 3645979 R 83332 CDS NP_217783.1 15610402 888704 complement(3646895..3647809) 1 NC_000962.3 Rv3266c, (MTCY71.06c), len: 304 aa. RmlD,dTDP-6-deoxy-L-lyxo-4-hexulose reductase (dTDP-rhamnose modification protein) (see citations below), highly similar to Q9CCK8 putative dTDP-rhamnose modification protein from Mycobacterium leprae (311 aa), FASTA scores, opt: 1440,E(): 1.1e-78, (74.7% identity in 312 aa overlap); and similar to several dTDP-4-dehydrorhamnose reductase e.g. STRL_STRGR|P29781 from Streptomyces griseus (304 aa), FASTA scores, opt: 788, E(): 0, (47.4% identity in 304 aa overlap).; dTDP-6-deoxy-L-lyxo-4-hexulose reductase RmlD (dTDP-rhamnose modification protein) (dTDP-rhamnose biosynthesis protein) (dTDP-rhamnose synthase) 3647809 rmlD 888704 rmlD Mycobacterium tuberculosis H37Rv dTDP-6-deoxy-L-lyxo-4-hexulose reductase RmlD (dTDP-rhamnose modification protein) (dTDP-rhamnose biosynthesis protein) (dTDP-rhamnose synthase) NP_217783.1 3646895 R 83332 CDS NP_217784.1 15610403 888713 3647885..3649381 1 NC_000962.3 Rv3267, (MTCY71.07), len: 498 aa. Conserved protein,CPSA-related protein, equivalent to Q9CCK9|ML0750 hypothetical protein from Mycobacterium leprae (489 aa),FASTA scores: opt: 2523, E(): 5e-138, (78.9% identity in 498 aa overlap); and Q50160|CPSA (hypothetical protein CPSA) from Mycobacterium leprae (516 aa), FASTA scores: opt: 868, E(): 1.2e-42, (34.7% identity in 507 aa overlap). Also similar to O06347|CPSA|Rv3484|MTCY13E12.37 CPSA from Mycobacterium tuberculosis (512 aa), FASTA scores: opt: 928, E(): 4.2e-46, (37.35% identity in 498 aa overlap); and O53834|Rv0822c|MTV043.14c hypothetical 72.9 KDA protein from Mycobacterium tuberculosis (684 aa), FASTA scores: opt: 434, E(): 1.5e-17, (30.9% identity in 541 aa overlap). Also similar to Q9KZK0|SCE34.26 conserved hypothetical protein from Streptomyces coelicolor (507 aa), FASTA scores: opt: 437, E(): 8.1e-18, (28.55% identity in 469 aa overlap); O68907 FRNA protein from Streptomyces roseofulvus (770 aa), FASTA scores: opt: 388, E(): 7.6e-15, (32.6% identity in 267 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A. Predicted to be an outer membrane protein (See Song et al., 2008).; Conserved protein (CPSA-related protein) 3649381 888713 Rv3267 Mycobacterium tuberculosis H37Rv Conserved protein (CPSA-related protein) NP_217784.1 3647885 D 83332 CDS NP_217785.3 448824805 888703 3649420..3650109 1 NC_000962.3 Rv3268, (MTCY71.08), len: 229 aa. Conserved hypothetical protein, similar to Q9KZK4|SCE34.22 hypothetical 27.1 KDA protein from Streptomyces coelicolor (263 aa), FASTA scores: opt: 442, E(): 5.9e-20, (40.1% identity in 242 aa overlap). Also weak similarity to N-terminal part (approximately 1530 to 1740 residues) of O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 159, E(): 0.11, (30.35% identity in 224 aa overlap).; hypothetical protein 3650109 888703 Rv3268 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217785.3 3649420 D 83332 CDS NP_217786.1 15610405 888709 3650234..3650515 1 NC_000962.3 Rv3269, (MTCY71.09), len: 93 aa. Conserved protein,similar to many Mycobacterium proteins and chaperonins/heat shock proteins e.g. Q9CCL0|ML0748 hypothetical protein from Mycobacterium leprae (92 aa), FASTA scores: opt: 427, E(): 6.8e-21, (73.65% identity in 91 aa overlap); Q10865|Rv1993c|MT2049|MTCY39.26c hypothetical protein from Mycobacterium tuberculosis (90 aa), FASTA scores: opt: 313,E(): 1.2e-13, (60.7% identity in 84 aa overlap); P71542|Y968_MYCTU|Rv0968|MTCY10D7.06c (98 aa), FASTA scores: opt: 294, E(): 2.2e-12, (55.1% identity in 98 aa overlap); Q50827|MOPA|GROEL|CH60_MYCVA chaperonin (protein CPN60) from Mycobacterium vaccae (120 aa), FASTA scores: opt: 107, E(): 2.1, (39.5% identity in 81 aa overlap); Q9AEB3|HSP65 heat shock protein (fragment) from Mycobacterium gadium (122 aa), FASTA scores: opt: 102, E(): 4.4, (38.25% identity in 81 aa overlap); Q49374|CH60_MYCGN|MOPA|GROEL chaperonin (protein CPN60) from Mycobacterium genavense (120 aa), FASTA scores: opt: 99, E(): 6.8, (40.25% identity in 82 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3650515 888709 Rv3269 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217786.1 3650234 D 83332 CDS NP_217787.1 15610406 888705 3650526..3652682 1 NC_000962.3 Rv3270, (MT3370, MTCY71.10), len: 718 aa. Probable ctpC, metal cation-transport ATPase P-type, integral membrane protein, equivalent to Q9CCL1|CTPC|ML0747 putative cation transport ATPase from Mycobacterium leprae (725 aa),FASTA scores: opt: 3908, E(): 0, (85.95% identity in 713 aa overlap). Also similar to O66027|MTAA metal transporting ATPase MTA72 from Mycobacterium tuberculosis (680 aa),FASTA scores: opt: 3756, E(): 5.5e-213, (91.45% identity in 679 aa overlap); and to other ATPases e.g. Q9ZHC7|SILP_SALTY putative cation transporting P-type ATPase from Salmonella typhimurium (824 aa), FASTA scores: opt: 1145, E(): 1.3e-59, (36.55% identity in 643 aa overlap); Q9HX93|PA3920 probable metal transporting P-type ATPase from Pseudomonas aeruginosa (792 aa), FASTA scores: opt: 1140, E(): 2.4e-59, (35.95% identity in 745 aa overlap); etc. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB.; Probable metal cation-transporting P-type ATPase C CtpC 3652682 ctpC 888705 ctpC Mycobacterium tuberculosis H37Rv Probable metal cation-transporting P-type ATPase C CtpC NP_217787.1 3650526 D 83332 CDS NP_217788.1 15610407 888716 complement(3652679..3653347) 1 NC_000962.3 Rv3271c, (MTCY71.11c), len: 222 aa. Probable conserved integral membrane protein, similar to others e.g. Q9RD35|SCM1.07c from Streptomyces coelicolor (230 aa),FASTA scores: opt: 360, E(): 4.7e-16, (33.85% identity in 195 aa overlap); Q9X897|SCE2.02c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 357, E(): 7.3e-16,(33.85% identity in 195 aa overlap); Q9D0E0 2610024A01RIK protein from Mus musculus (Mouse) (288 aa), FASTA scores: opt: 191, E(): 3.7e-05, (23.65% identity in 207 aa overlap).; Probable conserved integral membrane protein 3653347 888716 Rv3271c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217788.1 3652679 R 83332 CDS NP_217789.1 15610408 888702 3653448..3654632 1 NC_000962.3 Rv3272, (MTCY71.12), len: 394 aa. Conserved hypothetical protein, similar to various proteins e.g. Q9I672|PA0446 hypothetical protein from Pseudomonas aeruginosa (407 aa), FASTA scores: opt: 643, E(): 6.8e-32,(33.15% identity in 389 aa overlap); Q9RJU8|SCF41.21 putative racemase from Streptomyces coelicolor (403 aa),FASTA scores: opt: 541, E(): 1.1e-25, (31.95% identity in 385 aa overlap); O87838|SC8A6.04c putative transferase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 539,E(): 1.5e-25, (29.95% identity in 395 aa overlap); Q9I563|PA0882 from Pseudomonas aeruginosa (400 aa), FASTA scores: opt: 530, E(): 5.2e-25, (28.8% identity in 396 aa overlap); BAB60328|TVG1215416 L-carnitine dehydratase from Thermoplasma volcanium (399 aa), FASTA scores: opt: 529,E(): 6e-25, (32.9% identity in 383 aa overlap); etc. C-terminus is similar to Q49678|U00012_27|B1308_C3_195 from Mycobacterium leprae (130 aa) (60.0% identity in 115 aa overlap). Also partially similar to MTCY359_7 from M. tuberculosis (778 aa) (29.9% identity in 388 aa overlap).; hypothetical protein 3654632 888702 Rv3272 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217789.1 3653448 D 83332 CDS NP_217790.1 15610409 888700 3654637..3656931 1 NC_000962.3 Rv3273, (MTCY71.13), len: 764 aa. Probable transmembrane protein (N-terminal part is hydrophobic) with probable carbonic anhydrase activity (in C-terminal part). Possibly involved in transport of sulfate. Equivalent to Q9CBA3|ML2279 putative transmembrane transport protein from Mycobacterium leprae (496 aa), FASTA scores: opt: 1637,E(): 1.8e-89, (59.15% identity in 487 aa overlap). Similar to various proteins (principally sulfate transporters) e.g. Q9X927|SCH5.25 putative integral membrane protein from Streptomyces coelicolor (830 aa), FASTA scores: opt: 1325,E(): 8e-71, (40.85% identity in 788 aa overlap); Q9I729|PA0103 probable sulfate transporter from Pseudomonas aeruginosa (523 aa), FASTA scores: opt: 1015, E(): 1.3e-52,(39.95% identity in 488 aa overlap); Q9KN88|VCA0077 sulfate permease family protein from Vibrio cholerae (553 aa),FASTA scores: opt: 629, E(): 9.6e-30, (30.95% identity in 423 aa overlap); etc. C-terminal part (aa 550-764) shows similarity to carbonic anhydrase e.g. P27134|CYNT_SYNP7 carbonic anhydrase (272 aa), FASTA scores: opt: 350, E(): 8.1e-15, (33.8% identity in 201 aa overlap). Contains PS00704 Prokaryotic-type carbonic anhydrases signature 1. Seems to belong to the SulP family.; Probable transmembrane carbonic anhydrase (carbonate dehydratase) (carbonic dehydratase) 3656931 888700 Rv3273 Mycobacterium tuberculosis H37Rv Probable transmembrane carbonic anhydrase (carbonate dehydratase) (carbonic dehydratase) NP_217790.1 3654637 D 83332 CDS NP_217791.1 15610410 888731 complement(3656920..3658089) 1 NC_000962.3 Rv3274c, (MTCY71.14c), len: 389 aa. Probable fadE25,Acyl-CoA Dehydrogenase, equivalent to P46703|ACDP_MYCLE|FADE25|ACD|ML0737|B1308_F1_34 probable acyl-CoA dehydrogenase FADE25 from Mycobacterium leprae (389 aa), FASTA scores: opt: 2394, E(): 3.8e-143, (92.05% identity in 389 aa overlap). Also similar to many e.g. Q9RIQ5|fade fatty acid acyl-CoA dehydrogenase from Streptomyces lividans (385 aa), FASTA scores: opt: 1692,E(): 4.9e-99, (67.35% identity in 383 aa overlap); P45867|ACDA_BACSU|ACD from Bacillus subtilis (379 aa),FASTA scores: opt: 1212, E(): 7.2e-69, (51.85% identity in 376 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 1209, E(): 1.1e-68,(51.7% identity in 377 aa overlap); P52042|ACDS_CLOAB|BCD from Clostridium acetobutylicum (379 aa), FASTA scores: opt: 1056, E(): 4.6e-59, (44.6% identity in 379 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE25 3658089 fadE25 888731 fadE25 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE25 NP_217791.1 3656920 R 83332 CDS NP_217792.1 15610411 888721 complement(3658114..3658638) 1 NC_000962.3 Rv3275c, (MTCY71.15c, PUR6), len: 174 aa. Probable purE, phosphoribosylaminoimidazole carboxylase catalytic subunit, equivalent to P46702|PUR6_MYCLE|pure|ML0736|B1308_F3_98 from Mycobacterium leprae (171 aa), FASTA scores: opt: 878, E(): 1.5e-43, (81.55% identity in 168 aa overlap). Also similar to others e.g. Q9AXD0|AIRC from Nicotiana tabacum (Common tobacco) (623 aa), FASTA scores: opt: 712, E(): 1.4e-33,(69.35% identity in 160 aa overlap) (similarity in C-terminal part for this one); Q44679|PUR6_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (177 aa), FASTA scores: opt: 651, E(): 1.5e-30, (68.25% identity in 148 aa overlap); Q55498|PUR6_SYNY3|pure|SLL0901 from Synechocystis sp. strain PCC 6803 (176 aa), FASTA scores: opt: 639, E(): 7.1e-30, (60.5% identity in 167 aa overlap); etc.; Probable phosphoribosylaminoimidazole carboxylase catalytic subunit PurE (air carboxylase) (AIRC) 3658638 purE 888721 purE Mycobacterium tuberculosis H37Rv Probable phosphoribosylaminoimidazole carboxylase catalytic subunit PurE (air carboxylase) (AIRC) NP_217792.1 3658114 R 83332 CDS NP_217793.1 15610412 888720 complement(3658635..3659924) 1 NC_000962.3 Rv3276c, (MTCY71.16c), len: 429 aa. Probable purK,phosphoribosylaminoimidazole carboxylase ATPase subunit ,equivalent to P46701|PURK_MYCLE|ML0735|B1308_F1_32 phosphoribosylaminoimidazole carboxylase ATPase subunit from Mycobacterium leprae (439 aa), FASTA scores: opt: 2168, E(): 2.3e-123, (76.15% identity in 444 aa overlap). Also similar to others e.g. Q44678|PURK_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (413 aa), FASTA scores: opt: 1179, E(): 9.1e-64, (48.35% identity in 389 aa overlap); Q9KZ85|PURK from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1150, E(): 4.7e-62,(55.35% identity in 345 aa overlap); Q54975|PURK_SYNP7 from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (395 aa), FASTA scores: opt: 772, E(): 3e-39, (38.1% identity in 383 aa overlap); etc. Belongs to the PurK / PurT family.; Probable phosphoribosylaminoimidazole carboxylase ATPase subunit PurK (air carboxylase) (AIRC) 3659924 purK 888720 purK Mycobacterium tuberculosis H37Rv Probable phosphoribosylaminoimidazole carboxylase ATPase subunit PurK (air carboxylase) (AIRC) NP_217793.1 3658635 R 83332 CDS NP_217794.1 15610413 888729 3659878..3660696 1 NC_000962.3 Rv3277, (MTCY71.17), len: 272 aa. Probable conserved transmembrane protein, equivalent, but longer 49 aa, to Q49673|B1308_C1_121|ML0734 putative membrane protein from Mycobacterium leprae (228 aa), FASTA scores: opt: 1266,E(): 6.1e-78, (84.2% identity in 228 aa overlap). Also similar to various proteins (principally unknowns) e.g. Q9KZ84|SCE25.02 putative integral membrane protein from Streptomyces coelicolor (190 aa), FASTA scores: opt: 197,E(): 3.6e-06, (32.0% identity in 150 aa overlap); BAB50058|MLL3086 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (136 aa), FASTA scores: opt: 176, E(): 6.9e-05, (34.7% identity in 147 aa overlap); O29640|AF0615 hypothetical protein from Archaeoglobus fulgidus (129 aa),FASTA scores: opt: 120, E(): 0.38, (23.35% identity in 120 aa overlap); Q9KJU8|GTCA teichoic acid glycosylation protein from Listeria innocua (145 aa), FASTA scores: opt: 117, E(): 0.67, (23.85% identity in 151 aa overlap); etc. Equivalent to AAK47718 from Mycobacterium tuberculosis strain CDC1551 (256 aa) but longer 16 aa. Contains PS00044 Bacterial regulatory proteins, lysR family signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 3660696 888729 Rv3277 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217794.1 3659878 D 83332 CDS NP_217795.1 15610414 888724 complement(3660651..3661169) 1 NC_000962.3 Rv3278c, (MTCY71.18c), len: 172 aa. Probable conserved transmembrane protein, equivalent to Q9CCL2|ML0733 putative membrane protein from Mycobacterium leprae (172 aa), FASTA scores: opt: 1024, E(): 6e-61,(83.15% identity in 172 aa overlap); and Q49672|B1308_F2_67 hypothetical protein from Mycobacterium leprae (181 aa),FASTA scores: opt: 1024, E(): 6.3e-61, (83.15% identity in 172 aa overlap) (this is certainly the same putative protein but with N-terminus longer). Also some similarity to other hypothetical proteins (generally membrane proteins) e.g. O26822|MTH726 hypothetical protein from Methanobacterium thermoautotrophicum (204 aa), FASTA scores: opt: 147, E(): 0.0079, (24.6% identity in 187 aa overlap); Q9X8H4|SCE9.01 hypothetical 47.7 KDA protein (fragment) from Streptomyces coelicolor (436 aa), FASTA scores: opt: 151, E(): 0.0079, (28.1% identity in 153 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 3661169 888724 Rv3278c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217795.1 3660651 R 83332 CDS NP_217796.1 15610415 888726 complement(3661212..3662012) 1 NC_000962.3 Rv3279c, (MTCY71.19c), len: 266 aa. Possible birA,bifunctional protein: biotin operon repressor and biotin--[acetyl-CoA-carboxylase] synthetase, equivalent to Q9CCL3|BIRA|ML0732 biotin APO-protein ligase from Mycobacterium leprae (274 aa), FASTA scores: opt: 1189,E(): 2.3e-66, (71.2% identity in 271 aa overlap). But as it lacks a BirA h-t-h domain at N-terminus, may simply be biotin apo-protein ligase. Also similar to others e.g. Q9CNX6|BIRA|PM0296 from Pasteurella multocida (312 aa),FASTA scores: opt: 347, E(): 2.7e-14, (32.95% identity in 270 aa overlap); Q9HWC0|BIRA|PA4280 from Pseudomonas aeruginosa (312 aa), FASTA scores: opt: 335, E(): 1.5e-13,(34.2% identity in 272 aa overlap); Q9A6Z0|CC1936 from Caulobacter crescentus (250 aa), FASTA scores: opt: 332,E(): 1.9e-13, (33.6% identity in 238 aa overlap); P06709|BIRA_ECOLI (321 aa), FASTA scores: opt: 314, E(): 3.1e-12, (34.15% identity in 249 aa overlap); etc. Similar with other bacterial BIRA and with eukaryotic biotin APO-protein ligase.; Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligase) 3662012 birA 888726 birA Mycobacterium tuberculosis H37Rv Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligase) NP_217796.1 3661212 R 83332 CDS NP_217797.1 15610416 888725 3662062..3663708 1 NC_000962.3 Rv3280, (MTCY71.20, pccB), len: 548 aa. Probable accD5, propionyl-CoA carboxylase beta chain 5, equivalent to P53002|PCCB_MYCLE|ACCD5|ML0731|B1308_C1_125 probable propionyl-CoA carboxylase beta chain 5 from Mycobacterium leprae (549 aa), FASTA scores: opt: 3241, E(): 4e-192,(88.7% identity in 549 aa overlap). Also similar to many e.g. O87201|DTSR2 DTSR2 protein involved in glutamate production from Corynebacterium glutamicum (Brevibacterium flavum) (537 aa), FASTA scores: opt: 2604, E(): 6.9e-153,(74.1% identity in 529 aa overlap) (see Kimura et al.,1996); P53003|PCCB_SACER from Saccharopolyspora erythraea (Streptomyces erythraeus) (546 aa), FASTA scores: opt: 2466, E(): 2.2e-144, (70.2% identity in 530 aa overlap); O88155|DTSR1 DTSR1 protein from Corynebacterium glutamicum (Brevibacterium flavum) (543 aa), FASTA scores: opt: 2375,E(): 8.8e-139, (67.1% identity in 529 aa overlap; Q9X4K7|PCCB from Streptomyces coelicolor (530 aa), FASTA scores: opt: 2360, E(): 7.3e-138, (67.9% identity in 533 aa overlap); O24789|mxpccb from Myxococcus xanthus (524 aa),FASTA scores: opt: 1868, E(): 1.5e-107, (56.85% identity in 524 aa overlap); etc. Also similar with methylmalonyl-CoA decarboxylases e.g. O59018|PH1287 from Pyrococcus horikoshii (522 aa), FASTA scores: opt: 1841, E(): 6.7e-106, (54.15% identity in 528 aa overlap). Also similarity with MTCY427.28 (43.8% identity in 434 aa overlap). Belongs to the ACCD/PCCB family. AccA3 (Rv3285),AccD5 (Rv3280), AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006).; Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase) 3663708 accD5 888725 accD5 Mycobacterium tuberculosis H37Rv Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase) NP_217797.1 3662062 D 83332 CDS NP_217798.1 15610417 888732 3663689..3664222 1 NC_000962.3 Rv3281, (MTCY71.21), len: 177 aa. Probable accE5,bifunctional acetyl-/propionyl-coenzyme A carboxylase,epsilon chain, equivalent (but longer 14 aa and with a gap between aa 82-102) to AAK47723|MT3380 from Mycobacterium tuberculosis strain CDC1551 (142 aa), FASTA scores: opt: 830, E(): 3.1e-40, (86.5% identity in 163 aa overlap). C-terminus highly similar to Q49671|B1308_C3_211|ML0730 from Mycobacterium leprae (84 aa), FASTA scores: opt: 393,E(): 7.6e-16, (68.95% identity in 87 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). AccA3 (Rv3285), AccD5 (Rv3280),AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006).; Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (epsilon chain) AccE5 3664222 accE5 888732 accE5 Mycobacterium tuberculosis H37Rv Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (epsilon chain) AccE5 NP_217798.1 3663689 D 83332 CDS NP_217799.1 15610418 888718 3664219..3664887 1 NC_000962.3 Rv3282, (MTCY71.22), len: 222 aa. Conserved hypothetical protein, equivalent to Q49670|ML0729 1308R (hypothetical protein ML0729) from Mycobacterium leprae (213 aa), FASTA scores: opt: 945, E(): 5.5e-54, (68.55% identity in 213 aa overlap). Also similar to Q9EWV6|2SCK31.18 conserved hypothetical protein from Streptomyces coelicolor (206 aa), FASTA scores: opt: 459,E(): 1.3e-22, (47.35% identity in 209 aa overlap); P74331|MAF or SLL0905 MAF protein from Synechocystis sp. strain PCC 6803 (195 aa), FASTA scores: opt: 401, E(): 6.9e-19, (43.0% identity in 207 aa overlap); and shows weak similarity with various proteins e.g. Q9BUL6 acetylserotonin O-methyltransferase-like from Homo sapiens (Human) (621 aa), FASTA scores: opt: 282, E(): 8.9e-11,(31.6% identity in 193 aa overlap); O95671|ASMTL ASMTL protein from Homo sapiens (Human) (629 aa), FASTA scores: opt: 282, E(): 9e-11, (31.6% identity in 193 aa overlap); BAB51136|MLR4491 MAF protein from Rhizobium loti (Mesorhizobium loti) (199 aa), FASTA scores: opt: 269, E(): 2.3e-10, (29.3% identity in 198 aa overlap); etc.; hypothetical protein 3664887 888718 Rv3282 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217799.1 3664219 D 83332 CDS NP_217800.1 15610419 888717 3664928..3665821 1 NC_000962.3 Rv3283, (MTCY71.23), len: 297 aa. Probable sseA,thiosulfate sulfurtransferase, equivalent P46700|THT2_MYCLE|SSEA|ML0728|B1308_C1_127 putative thiosulfate sulfurtransferase SSEA from Mycobacterium leprae (296 aa), FASTA scores: opt: 1742, E(): 5.5e-108,(83.45% identity in 296 aa overlap). Also highly similar to others e.g. Q9RXT9|DR0217 from Deinococcus radiodurans (286 aa), FASTA scores: opt: 1057, E(): 1.2e-62, (53.86% identity in 273 aa overlap); P16385|THTR_SACER|CYSA from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa), FASTA scores: opt: 1006, E(): 2.7e-59, (51.25% identity in 277 aa overlap); P71121|THTR_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (225 aa), FASTA scores: opt: 897, E(): 3.6e-52, (59.05% identity in 215 aa overlap); etc. Also highly similar to O05793|CYSA1|CYSA|Rv3117|MT3199|MTCY164.27|CYSA2|RV0815c|M T0837|MTV043.07c|THTR_MYCTU putative thiosulfate sulfurtransferase from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 955, E(): 6.3e-56, (50.2% identity in 271 aa overlap); and Q50036|THTR_MYCLE|CYSA|CYSA3|ML2198 putative thiosulfate sulfurtransferase from Mycobacterium leprae (277 aa), FASTA scores: opt: 931, E(): 2.5e-54, (48.9% identity in 276 aa overlap). Shows some similarity to MTCY339.19c (30.3% identity in 254 aa overlap). Contains PS00683 Rhodanese C-terminal signature. Belongs to the rhodanese family. Thought to be differentially expressed within host cells (see Triccas et al., 1999).; Probable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) 3665821 sseA 888717 sseA Mycobacterium tuberculosis H37Rv Probable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) NP_217800.1 3664928 D 83332 CDS NP_217801.1 15610420 887966 3665818..3666249 1 NC_000962.3 Rv3284, (MTCY71.24, unknown), len: 143 aa. Conserved hypothetical protein, with similarity to other bacterial hypothetical proteins e.g. Q9RXU0|DR0216 from Deinococcus radiodurans (147 aa), FASTA scores: opt: 425, E(): 9.1e-21,(46.55% identity in 146 aa overlap); BAB37094|ECS3671 from Escherichia coli strain O157:H7 (147 aa), FASTA scores: opt: 187, E(): 2.2e-05, (29.5% identity in 139 aa overlap); AAG57925|YGDK from Escherichia coli strain O157:H7 EDL933 (147 aa), FASTA scores: opt: 187, E(): 2.2e-05, (32.05% identity in 139 aa overlap); etc.; hypothetical protein 3666249 887966 Rv3284 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217801.1 3665818 D 83332 CDS NP_217802.1 15610421 887912 3666357..3668159 1 NC_000962.3 Rv3285, (MTCY71.25), len: 600 aa. Probable accA3,bifunctional protein acetyl-/propionyl-coenzyme A carboxylase, alpha chain (see citations below) equivalent to P46392|BCCA_MYCLE|BCCA|ML0726|B1308_C1_129 acetyl-/propionyl-coenzyme A carboxylase alpha chain from Mycobacterium leprae (598 aa), FASTA scores: opt: 3510,E(): 1.1e-196, (89.3% identity in 601 aa overlap). Also highly similar to other proteins e.g. P71122|ACCBC acyl coenzyme A carboxylase from Corynebacterium glutamicum (Brevibacterium flavum) (591 aa), FASTA scores: opt: 2776,E(): 5.6e-154, (71.95% identity in 592 aa overlap); Q54119|BCPA2 biotin carboxylase and biotin carboxyl carrier protein from Saccharopolyspora erythraea (Streptomyces erythraeus) (591 aa), FASTA scores: opt: 2723, E(): 6.7e-151, (70.5% identity in 590 aa overlap); Q54105|BCPA biotin carboxylase and biotin carboxyl carrier protein from Saccharopolyspora erythraea (Streptomyces erythraeus) (597 aa), FASTA scores: opt: 2721, E(): 8.9e-151, (70.05% identity in 594 aa overlap); Q9EWV4|2SCK31.20 putative acyl-CoA carboxylase complex a subunit from Streptomyces coelicolor (590 aa), FASTA scores: opt: 2626, E(): 2.9e-145, (68.25% identity in 595 aa overlap); etc. Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2, PS00188 Biotin-requiring enzymes attachment site. Similar to other biotin-dependent enzymes and carbamoyl-phosphate synthetases. AccA3 (Rv3285), AccD5 (Rv3280), AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006).; Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (alpha chain) AccA3: biotin carboxylase + biotin carboxyl carrier protein (BCCP) 3668159 accA3 887912 accA3 Mycobacterium tuberculosis H37Rv Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (alpha chain) AccA3: biotin carboxylase + biotin carboxyl carrier protein (BCCP) NP_217802.1 3666357 D 83332 CDS NP_217803.1 15610422 888727 complement(3668169..3668954) 1 NC_000962.3 Rv3286c, (MTCY71.26), len: 261 aa. SigF, stress response/stationary phase RNA polymerase sigma factor (see citations below), similar to several Streptomyces RNA polymerase sigma factors e.g. Q9RPC8|sigh from Streptomyces coelicolor A3(2) (354 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9RIT0|SIG1 from Streptomyces coelicolor (361 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9ADM4|2SC10A7.38c from Streptomyces coelicolor (318 aa),FASTA scores: opt: 776, E(): 4.6e-40, (48.75% identity in 240 aa overlap); P37971|RPOF_STRCO|SIGF|RPOX|2SCD60.01c from Streptomyces coelicolor (287 aa), FASTA scores: opt: 717, E(): 1.6e-36, (44.5% identity in 245 aa overlap); P37970|RPOF_STRAU|SIGF|RPOX from Streptomyces aureofaciens (297 aa); etc. Contains possible helix-turn-helix motif at aa 229-250 (+7.38 SD). Similar to the sigma-70 factor family. Seems expressed in stationary phase and under stress conditions in vitro (see citations below).; Alternative RNA polymerase sigma factor SigF 3668954 sigF 888727 sigF Mycobacterium tuberculosis H37Rv Alternative RNA polymerase sigma factor SigF NP_217803.1 3668169 R 83332 CDS NP_217804.4 448824806 887977 complement(3668951..3669388) 1 NC_000962.3 Rv3287c, (MTCY71.27c), len: 145 aa. RsbW (alternate gene name: usfX), anti-sigma factor (see citations below),similar to Q49667|B1308_F3_89 from Mycobacterium leprae (75 aa), FASTA scores: opt: 308, E(): 2.5e-15, (72.2% identity in 72 aa overlap); Q9R3X8|PRS1|USHX|PRS PRS1 protein (anti-sigma factor) from Streptomyces coelicolor (137 aa),FASTA scores: opt: 184, E(): 3.7e-06, (36.8% identity in 106 aa overlap); O50231 putative sigma-B regulator from Bacillus licheniformis (160 aa), FASTA scores: opt: 122,E(): 0.13, (23.9% identity in 92 aa overlap); and P17904|RSBW_BACSU anti-sigma B factor (sigma-B negative effector RSBW) from Bacillus subtilis (160 aa), FASTA scores: opt: 108, E(): 1.3, (21.25% identity in 127 aa overlap). Equivalent to AAK47729 from Mycobacterium tuberculosis strain CDC1551 (145 aa) but longer 99 aa. Induction by heat shock, salt stress, oxidative stress,glucose limitation and oxygen limitation. N-terminus shortened since first submission (previously 242 aa). Binds ATP, GTP.; Anti-sigma factor RsbW (sigma negative effector) 3669388 rsbW 887977 rsbW Mycobacterium tuberculosis H37Rv Anti-sigma factor RsbW (sigma negative effector) NP_217804.4 3668951 R 83332 CDS NP_217805.1 15610424 888735 complement(3669586..3669999) 1 NC_000962.3 Rv3288c, (MTCY71.28c), len: 137 aa. UsfY, putative protein (see citation below). Has no significant homologues. May not be contranscribed with the usfX and sigF proteins.; Putative protein UsfY 3669999 usfY 888735 usfY Mycobacterium tuberculosis H37Rv Putative protein UsfY NP_217805.1 3669586 R 83332 CDS NP_217806.1 15610425 888736 complement(3670034..3670411) 1 NC_000962.3 Rv3289c, (MTCY71.29c), len: 125 aa. Possible transmembrane protein, showing slight similarity to other membrane proteins or glycoproteins.; Possible transmembrane protein 3670411 888736 Rv3289c Mycobacterium tuberculosis H37Rv Possible transmembrane protein NP_217806.1 3670034 R 83332 CDS NP_217807.1 15610426 887904 complement(3670445..3671794) 1 NC_000962.3 Rv3290c, (MTCY71.30), len: 449 aa. Probable lat,lysine-epsilon aminotransferase, similar to Q05174|LAT_NOCLA from Nocardia lactamdurans (450 aa), FASTA scores: opt: 1702, E(): 1.1e-99, (60.35% identity in 439 aa overlap); and Q01767|Q53823|LAT_STRCL from Streptomyces clavuligerus (457 aa), FASTA scores: opt: 1676, E(): 4.9e-98, (60.15% identity in 434 aa overlap). Also some similarity to 4-aminobutyrate aminotransferase proteins (gamma-amino-N-butyrate transaminases). Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate.; Probable L-lysine-epsilon aminotransferase Lat (L-lysine aminotransferase) (lysine 6-aminotransferase) 3671794 lat 887904 lat Mycobacterium tuberculosis H37Rv Probable L-lysine-epsilon aminotransferase Lat (L-lysine aminotransferase) (lysine 6-aminotransferase) NP_217807.1 3670445 R 83332 CDS NP_217808.1 15610427 888728 complement(3671845..3672297) 1 NC_000962.3 Rv3291c, (MTCY71.31c), len: 150 aa. Probable lrpA,transcriptional regulator Lrp/AsnC-family, similar to other regulatory proteins e.g. Q9RKY4|SC6D7.14 from Streptomyces coelicolor (165 aa), FASTA scores: opt: 503, E(): 9.1e-26,(50.35% identity in 143 aa overlap); Q9KYP0|SCD69.13 from Streptomyces coelicolor (167 aa), FASTA scores: opt: 310,E(): 2.7e-13, (37.2% identity in 129 aa overlap); BAB50701|MLL3910 from Rhizobium loti (Mesorhizobium loti) (152 aa), FASTA scores: opt: 282, E(): 1.6e-11, (39.55% identity in 129 aa overlap); O87635|LRP_KLEAE from Klebsiella aerogenes (163 aa), FASTA scores: opt: 279, E(): 2.5e-11, (38.1% identity in 147 aa overlap); etc. Contains helix-turn-helix motif at aa 22-43 (+3.94 SD). Could belong to the Lrp/AsnC family of transcriptional regulators.; Probable transcriptional regulatory protein LrpA (Lrp/AsnC-family) 3672297 lrpA 888728 lrpA Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein LrpA (Lrp/AsnC-family) NP_217808.1 3671845 R 83332 CDS NP_217809.1 15610428 888734 3672328..3673575 1 NC_000962.3 Rv3292, (MTCY71.32), len: 415 aa. Conserved hypothetical protein, similar to P76097|YDCJ_ECOLI|B1423 hypothetical 51.0 KDA protein from Escherichia coli strain K12 (447 aa), FASTA scores: opt: 747, E(): 5.6e-39, (38.55% identity in 449 aa overlap); BAB35451|ECS2028 hypothetical 51.0 KDA protein from Escherichia coli strain O157:H7 (447 aa), FASTA scores: opt: 744, E(): 8.6e-39, (38.3% identity in 449 aa overlap); AAG56352|Z2297 protein from Escherichia coli O157:H7 EDL933 (212 aa), FASTA scores: opt: 454, E(): 4.6e-21, (41.75% identity in 206 aa overlap); and similar in part with Q49664|B1308_C1_136 from Mycobacterium leprae (71 aa), FASTA scores: opt: 305, E(): 3.2e-12, (70.0% identity in 70 aa overlap).; hypothetical protein 3673575 888734 Rv3292 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217809.1 3672328 D 83332 CDS YP_177953.1 57117084 888733 3673602..3675086 1 NC_000962.3 Rv3293, (MTCY71.33), len: 494 aa. Probable pcd,piperideine-6-carboxylic acid dehydrogenase, highly similar to others e.g. O85725|PCD semialdehyde dehydrogenase from Streptomyces clavuligerus (512 aa), FASTA scores: opt: 2214, E(): 6.7e-121, (68.75% identity in 496 aa overlap) (see Alexander & Jensen 1998); Q9I4U7|PA1027 probable aldehyde dehydrogenase from Pseudomonas aeruginosa (529 aa), FASTA scores: opt: 1984, E(): 1.4e-107, (64.5% identity in 493 aa overlap); BAB49892|MLL2867 aldehyde dehydrogenase from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 1964, E(): 2e-106, (62.8% identity in 476 aa overlap); Q9A8Y1|CC1216 aldehyde dehydrogenase from Caulobacter crescentus (507 aa), FASTA scores: opt: 1909, E(): 3.1e-103, (59.95% identity in 497 aa overlap); O54199|PCD piperideine-6-carboxilic acid dehydrogenase from Streptomyces clavuligerus (496 aa), FASTA scores: opt: 1748, E(): 6.4e-94, (60.6% identity in 467 aa overlap); and Q9F1U8|PCD piperideine-6-carboxylate dehydrogenase from 'Flavobacterium' lutescens (510 aa), FASTA scores: opt: 1656, E(): 1.4e-88, (54.05% identity in 481 aa overlap) (see Fujii et al., 2000); etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Note that ORF Rv3290c seems to encoded the putative lat enzyme. Note that previously known as aldB.; Probable piperideine-6-carboxilic acid dehydrogenase Pcd (piperideine-6-carboxylate dehydrogenase) 3675086 pcd 888733 pcd Mycobacterium tuberculosis H37Rv Probable piperideine-6-carboxilic acid dehydrogenase Pcd (piperideine-6-carboxylate dehydrogenase) YP_177953.1 3673602 D 83332 CDS YP_177954.1 57117085 3205051 complement(3675186..3675995) 1 NC_000962.3 Rv3294c, len: 269 aa. Conserved hypothetical protein, similar to several conserved hypothetical proteins from Mycobacterium tuberculosis: O07781|Rv0597c (411 aa),FASTA scores: opt: 682, E(): 3.6e-37, (44.85% identity in 243 aa overlap); O53329|Rv3179 (454 aa), FASTA scores: opt: 561, E(): 3.3e-29, (42.20% identity in 218 aa overlap); Q10849|YK08_MYCTU|Rv2008c (441 aa), FASTA scores: opt: 194,E(): 3.9e-05, (30.10% identity in 239 aa overlap). Also some similarity with proteins from other organisms. Replace previous Rv3294 on opposite strand.; hypothetical protein 3675995 3205051 Rv3294c Mycobacterium tuberculosis H37Rv hypothetical protein YP_177954.1 3675186 R 83332 CDS NP_217812.1 15610431 887180 3676066..3676731 1 NC_000962.3 Rv3295, (MTCY71.35), len: 221 aa. Probable transcriptional regulator TetR-family, equivalent to Q9CCL4|ML0717 putative TetR-family transcriptional regulator from Mycobacterium leprae (223 aa), FASTA scores: opt: 1260, E(): 7.2e-75, (85.45% identity in 220 aa overlap). Also highly similar to other streptomyces regulators e.g. Q9RD77|SCF43.11 from Streptomyces coelicolor (205 aa), FASTA scores: opt: 442, E(): 9.8e-22,(38.6% identity in 202 aa overlap); Q9RKY8|SC6D7.09 from Streptomyces coelicolor (220 aa), FASTA scores: opt: 215,E(): 5.9e-07, (31.85% identity in 135 aa overlap); Q9L0U5|SCD35.06 from Streptomyces coelicolor (240 aa),FASTA scores: opt: 214, E(): 7.4e-07, (28.2% identity in 156 aa overlap); etc. Similar to the TetR/AcrR family of transcriptional regulators. Contains potential helix-turn-helix motif at aa 33-54 (+4.42 SD).; Probable transcriptional regulatory protein (probably TetR-family) 3676731 887180 Rv3295 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably TetR-family) NP_217812.1 3676066 D 83332 CDS NP_217813.1 15610432 887503 3676775..3681316 1 NC_000962.3 Rv3296, (MTCY71.36), len: 1513 aa. Probable lhr,ATP-dependent helicase, similar to others e.g. P30015|LHR_ECOLI|RHLF|B1653 from Escherichia coli stain K12 (1538 aa), FASTA scores: opt: 2930, E(): 1.5e-159, (47.55% identity in 1569 aa overlap); AAG56642|LHR from Escherichia coli stain O157:H7 EDL933 (1538 aa), FASTA scores: opt: 2930, E(): 1.5e-159, (47.6% identity in 1561 aa overlap); O86821|SC7C7.16c from Streptomyces coelicolor (1690 aa),FASTA scores: opt: 2919, E(): 7e-159, (53.55% identity in 1703 aa overlap); Q9HYW9|PA3272 from Pseudomonas aeruginosa (1448 aa), FASTA scores: opt: 907, E(): 6.2e-44, (35.85% identity in 1512 aa overlap); etc. Similar to dead/DEAH box helicase family and to helicase C-terminal domain. Contains PS00017 ATP/GTP-binding site motif A and possible helix-turn-helix motif.; Probable ATP-dependent helicase Lhr (large helicase-related protein) 3681316 lhr 887503 lhr Mycobacterium tuberculosis H37Rv Probable ATP-dependent helicase Lhr (large helicase-related protein) NP_217813.1 3676775 D 83332 CDS NP_217814.1 15610433 887937 3681320..3682087 1 NC_000962.3 Rv3297, (MTCY71.37, MT3396), len: 255 aa. Probable nei, endonuclease VIII (see citation below), similar to others e.g. O86820|END8_STRCO|NEI|SC7C7.15c from Streptomyces coelicolor (276 aa), FASTA scores: opt: 770,E(): 1.2e-42, (50.35% identity in 268 aa overlap); P50465|END8_ECOLI|NEI|B0714 from Escherichia coli strain K12 (262 aa), FASTA scores: opt: 310, E(): 6.3e-13, (28.1% identity in 267 aa overlap); AAG55037|NEI from Escherichia coli strain O157:H7 EDL933 (263 aa), FASTA scores: opt: 301, E(): 2.4e-12, (27.7% identity in 267 aa overlap); etc. Belongs to the FPG family.; Probable endonuclease VIII Nei 3682087 nei 887937 nei Mycobacterium tuberculosis H37Rv Probable endonuclease VIII Nei NP_217814.1 3681320 D 83332 CDS NP_217815.1 15610434 887984 complement(3682110..3683024) 1 NC_000962.3 Rv3298c, (MTCY71.38c), len: 304 aa. Possible lpqC,esterase lipoprotein, equivalent to Q9CCL5|LPQC|ML0715 putative secreted hydrolase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1543, E(): 1.3e-87, (71.6% identity in 303 aa overlap); and Q49658|B1308_F2_43 tubulin family protein from Mycobacterium leprae (302 aa), FASTA scores: opt: 1541, E(): 1.7e-87, (72.0% identity in 300 aa overlap). Also similar to Q9I5Z3|PA0543 hypothetical protein from Pseudomonas aeruginosa (322 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.3% identity in 319 aa overlap); Q9F2K9|SCH63.19c putative secreted protein from Streptomyces coelicolor (348 aa), FASTA scores: opt: 394,E(): 5.5e-17, (30.25% identity in 334 aa overlap); etc. And similar to O86367|LPQP|Rv0671|MTCI376.03c from Mycobacterium tuberculosis strain H37Rv (280 aa), FASTA scores: opt: 519, E(): 9.8e-25, (39.25% identity in 275 aa overlap). Probably lipoprotein, esterase membrane-bound,with 18 aa signal sequence as it contains appropriately positioned (PS00013) Prokaryotic membrane lipoprotein lipid attachment site.; Possible esterase lipoprotein LpqC 3683024 lpqC 887984 lpqC Mycobacterium tuberculosis H37Rv Possible esterase lipoprotein LpqC NP_217815.1 3682110 R 83332 CDS NP_217816.1 15610435 887500 complement(3683051..3685963) 1 NC_000962.3 Rv3299c, (MTCI418A.01c, MTCY71.39c), len: 970 aa. Probable atsB, arylsulfatase, similar to P51691|ARS_PSEAE|ATSA|PA0183 (alias CAA88421|ATSA) from Pseudomonas aeruginosa (535 aa), FASTA scores: opt: 645,E(): 5.8e-31, (32.0% identity in 550 aa overlap); Q9L4Y2|ATSA from Klebsiella pneumoniae (577 aa), FASTA scores: opt: 504, E(): 1.7e-22, (26.3% identity in 566 aa overlap); and P20713|ATSA|ARS_KLEAE (precursor) from Klebsiella pneumoniae (464 aa), FASTA scores: opt: 502,E(): 1.8e-22, (26.85% identity in 451 aa overlap). Also similar to Mycobacterium tuberculosis proteins O06776|MTI376.13c|ATSD|Rv0663 (787 aa) (43.6% identity in 796 aa overlap) and P95059|MTCY210.30|ATSA|R0711 (787 aa) (38.4% identity in 797 aa overlap). Equivalent to AAK47741 from Mycobacterium tuberculosis strain CDC1551 (992 aa) but shorter 22 aa. Contains PS00523 Sulfatases signature 1 and PS01095 Chitinases family 18 active site signature. Belongs to the sulfatase family.; Probable arylsulfatase AtsB (aryl-sulfate sulphohydrolase) (sulfatase) 3685963 atsB 887500 atsB Mycobacterium tuberculosis H37Rv Probable arylsulfatase AtsB (aryl-sulfate sulphohydrolase) (sulfatase) NP_217816.1 3683051 R 83332 CDS NP_217817.1 15610436 887958 complement(3685983..3686900) 1 NC_000962.3 Rv3300c, (MTCI418A.02c), len: 305 aa. Conserved hypothetical protein, similar to various proteins (notably pseudoridine synthase family proteins) e.g. Q9RJ76|SCI41.08 putative ribosomal pseudouridine synthase from Streptomyces coelicolor (324 aa), FASTA scores: opt: 876, E(): 4.5e-48,(52.1% identity in 313 aa overlap); Q9I272|PA2043 hypothetical protein from Pseudomonas aeruginosa (300 aa),FASTA scores: opt: 676, E(): 1.8e-35, (42.55% identity in 268 aa overlap); Q9JZW8|NMB0867 YABO/YCEC/SFHB family protein from Neisseria meningitidis (serogroup B) (307 aa),FASTA scores: opt: 597, E(): 1.8e-30, (42.9% identity in 282 aa overlap); Q9JUY2|NMA1085 hypothetical protein from Neisseria meningitidis (serogroup A) (307 aa), FASTA scores: opt: 597, E(): 1.8e-30, (42.9% identity in 282 aa overlap); Q12362|RIB2_YEAST|RIB2|YOL066C DRAP deaminase (pseudouridine synthase family protein) from Saccharomyces cerevisiae (Baker's yeast) (591 aa), FASTA scores: opt: 338, E(): 6.9e-14, (32.95% identity in 246 aa overlap); Q9RTS2|DR1684 putative pseudouridine synthase from Deinococcus radiodurans (321 aa), FASTA scores: opt: 319,E(): 6.5e-13, (32.75% identity in 235 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10786|Y04P_MYCTU|MTCY48.25c|Rv1540|MT1592 (308 aa) (28.8% identity in 299 aa overlap).; hypothetical protein 3686900 887958 Rv3300c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217817.1 3685983 R 83332 CDS NP_217818.1 15610437 887212 complement(3686912..3687577) 1 NC_000962.3 Rv3301c, (MTCI418A.03c), len: 221 aa. Probable phoY1, phosphate-transport system regulatory protein,highly similar to Q50047|phoY|PHOU1|PHOY1|ML2188 phosphate transport system protein PHOU homolog 1 from Mycobacterium leprae (222 aa), FASTA scores: opt: 929, E(): 7.8e-51,(61.45% identity in 218 aa overlap). Also highly similar to Q9FCE2|2SCD46.42c putative regulatory protein (fragment) from Streptomyces coelicolor (123 aa), FASTA scores: opt: 324, E(): 1.8e-13, (43.65% identity in 103 aa overlap); Q9L0R3|SCD8A.01c putative phosphate transport system regulatory protein (fragment) from Streptomyces coelicolor (139 aa), FASTA scores: opt: 309, E(): 1.7e-12, (36.7% identity in 139 aa overlap); Q52989|PHOU_RHIME phosphate transport system protein from Rhizobium meliloti (Sinorhizobium meliloti) (237 aa), FASTA scores: opt: 292,E(): 3.1e-11, (26.3% identity in 213 aa overlap); etc. And highly similar to Mycobacterium tuberculosis O53833|PHU2_MYCTU|MTV043_13c|PHOU2|PHOY2|Rv0821c|MT0843 phosphate transport system protein PHOU homolog 2 (213 aa) (63.4% identity in 213 aa overlap). Belongs to the PHOU family.; Probable phosphate-transport system transcriptional regulatory protein PhoU homolog 1 PhoY1 3687577 phoY1 887212 phoY1 Mycobacterium tuberculosis H37Rv Probable phosphate-transport system transcriptional regulatory protein PhoU homolog 1 PhoY1 NP_217818.1 3686912 R 83332 CDS NP_217819.1 15610438 887211 complement(3687685..3689442) 1 NC_000962.3 Rv3302c, (MTCI418A.04c, MTV016.01c), len: 585 aa. Probable glpd2, glycerol-3-phosphate dehydrogenase,equivalent to P53435|GLPD_MYCLE|ML0713|L308_C1_179 glycerol-3-phosphate dehydrogenase from Mycobacterium leprae (585 aa), FASTA scores: opt: 3489, E(): 2.2e-198,(90.75% identity in 584 aa overlap). Also highly similar to many e.g. Q9L0I3|SCD63.06 from Streptomyces coelicolor (568 aa), FASTA scores: opt: 2203, E(): 1.6e-122, (59.95% identity in 564 aa overlap); Q9RVK8|DR1019 from Deinococcus radiodurans (522 aa), FASTA scores: opt: 949, E(): 1.4e-48,(37.0% identity in 538 aa overlap); BAB53412|MLR7270 from Rhizobium loti (Mesorhizobium loti) (505 aa), FASTA scores: opt: 861, E(): 2.2e-43, (37.3% identity in 488 aa overlap); P18158|GLPD_BACSU from B. subtilis (555 aa), FASTA scores: opt: 768, E(): 7.2e-38, (32.85% identity in 484 aa overlap); etc. Also similar to Mycobacterium tuberculosis protein Q10502|GLPD_MYCTU|MTCY427_31c|Rv2249c glycerol-3-phosphate dehydrogenase (516 aa), FASTA scores: opt: 843, E(): 2.6e-42, (36.5% identity in 515 aa overlap). Contains PS00978 FAD-dependent glycerol-3-phosphate dehydrogenase signature 2. Cofactor: FAD (by similarity). Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.; Probable glycerol-3-phosphate dehydrogenase GlpD2 3689442 glpD2 887211 glpD2 Mycobacterium tuberculosis H37Rv Probable glycerol-3-phosphate dehydrogenase GlpD2 NP_217819.1 3687685 R 83332 CDS NP_217820.1 15610439 887659 complement(3689457..3690938) 1 NC_000962.3 Rv3303c, (MTV016.02c), len: 493 aa. Probable lpdA,quinone reductase, similar to e.g. Q9EWV3|2SCK31.22c putative oxidoreductase from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1420, E(): 2.4e-77, (54.9% identity in 471 aa overlap); Q9A7J2|CC1731 lipoamide dehydrogenase (E3 component,pyruvate dehydrogenase complex) from Caulobacter crescentus (466 aa), FASTA scores: opt: 696,E(): 3.6e-34, (29.6% identity in 463 aa overlap); Q04829|LPD|DLDH_HALVO dihydrolipoamide dehydrogenase from Halobacterium volcanii (Haloferax volcanii) (474 aa), FASTA scores: opt: 675, E(): 6.5e-33, (29.3% identity in 471 aa overlap); P50970|DLDH_ZYMMO|LPD dihydrolipoamide dehydrogenase from Zymomonas mobilis, FASTA scores: opt: 658, E(): 6.6e-32, (30.4% identity in 464 aa overlap); etc. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Cofactor: FAD.; NAD(P)H quinone reductase LpdA 3690938 lpdA 887659 lpdA Mycobacterium tuberculosis H37Rv NAD(P)H quinone reductase LpdA NP_217820.1 3689457 R 83332 CDS NP_217821.1 15610440 887605 3691141..3691620 1 NC_000962.3 Rv3304, (MTV016.03), len: 159 aa. Conserved protein,very similar to Q9CCL6|ML0711 hypothetical protein from Mycobacterium leprae (159 aa), FASTA scores: opt: 1041,E(): 6.1e-62, (91.8% identity in 159 aa overlap); and Q49927|L308_F3_97 from M. leprae (174 aa), FASTA scores: opt: 974, E(): 1.8e-57, (91.2% identity in 149 aa overlap). Also highly similar to Q9AD81|SCK13.10c conserved hypothetical protein from Streptomyces coelicolor (145 aa),FASTA scores: opt: 615, E(): 7.8e-34, (60.55% identity in 147 aa overlap); and shows some similarity to other various hypotheticals proteins. ORF continues upstream with possible start at 2198 (equivalent to AAK47746 from Mycobacterium tuberculosis strain CDC1551 (212 aa) but shorter 53 aa).; hypothetical protein 3691620 887605 Rv3304 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217821.1 3691141 D 83332 CDS YP_177955.1 57117086 887545 complement(3691639..3692808) 1 NC_000962.3 Rv3305c, (MTV016.04c), len: 389 aa. Possible amiA1,N-acyl-L-amino acid amidohydrolase (or peptidase), similar to many proteins e.g. Q9AK43|2SCK8.09 putative peptidase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1015, E(): 3.9e-54, (50.8% identity in 374 aa overlap); Q9UZ30|PAB0873 amino acid amidohydrolase from Pyrococcus abyssi (383 aa), FASTA scores: opt: 823, E(): 1.6e-42,(38.2% identity in 369 aa overlap); O58453|PH0722 long hypothetical amino acid amidohydrolase from Pyrococcus horikoshii (388 aa), FASTA scores: opt: 815, E(): 4.8e-42,(38.75% identity in 369 aa overlap); O34980|YTNL_BACSU hypothetical 45.2 KDA protein from B. subtilis (416 aa),FASTA scores: opt: 805, E(): 2.1e-41, (37.85% identity in 367 aa overlap); Q9KCF8|BH1613 N-acyl-L-amino acid amidohydrolase from Bacillus halodurans (404 aa), FASTA scores: opt: 795, E(): 8.1e-41, (37.7% identity in 382 aa overlap); BAB50445|MLR3583 hypothetical hippurate hydrolase from Rhizobium loti (Mesorhizobium loti) (387 aa), FASTA scores: opt: 761, E(): 8.9e-39, (37.65% identity in 385 aa overlap); Q9RXH4|DR0339 putative N-acyl-L-amino acid amidohydrolase from Deinococcus radiodurans (392 aa), FASTA scores: opt: 745, E(): 8.4e-38, (36.15% identity in 379 aa overlap); etc. Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site. Note that previously known as amiA.; Possible N-acyl-L-amino acid amidohydrolase AmiA1 (N-acyl-L-amino acid aminohydrolase) 3692808 amiA1 887545 amiA1 Mycobacterium tuberculosis H37Rv Possible N-acyl-L-amino acid amidohydrolase AmiA1 (N-acyl-L-amino acid aminohydrolase) YP_177955.1 3691639 R 83332 CDS YP_177956.1 57117087 887645 complement(3692805..3693989) 1 NC_000962.3 Rv3306c, (MTV016.05c), len: 394 aa. Probable amiB1,aminohydrolase, similar to several belonging to peptidase family M40 (and to hypothetical proteins) e.g. P54983|AMHX_BACSU amidohydrolase AMHX from Bacillus subtilis (389 aa), FASTA scores: opt: 286, E(): 9.9e-10,(26.6% identity in 351 aa overlap); P76052|ABGB_ECOLI Aminobenzoyl-glutamate utilizatio from Escherichia coli (481 aa), FASTA scores: opt: 383, E(): 2.1e-15, (30.5% identity in 328 aa overlap); P44765|YDAJ_HAEIN hypothetical protein HI0584 from Haemophilus influenzae (423 aa), FASTA scores: opt: 297, E(): 2.4e-10, (29.6% identity in 274 aa overlap). Note that previously known as amiB.; Probable amidohydrolase AmiB1 (aminohydrolase) 3693989 amiB1 887645 amiB1 Mycobacterium tuberculosis H37Rv Probable amidohydrolase AmiB1 (aminohydrolase) YP_177956.1 3692805 R 83332 CDS NP_217824.1 15610443 887542 3694054..3694860 1 NC_000962.3 Rv3307, (MTV016.06), len: 268 aa. Probable deoD (alternate gene name: punA), purine nucleoside phosphorylase, similar to others especially P46862|PUNA_MYCLE|DEOD_MYCLE|ML0707|L308_F2_56 from M. leprae (268 aa), FASTA scores: opt: 1373, E(): 1.5e-74,(82.05% identity in 262 aa overlap); Q9EWV2|2SCK31.24 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 1026,E(): 6.4e-54, (60.5% identity in 266 aa overlap); P81989|PUNA_CELSP from Cellulomonas sp (282 aa), FASTA scores: opt: 963, E(): 3.6e-50, (58.9% identity in 270 aa overlap); Q9X1T2|TM1596 from Thermotoga maritima (265 aa),FASTA scores: opt: 584, E(): 1.1e-27, (39.55% identity in 263 aa overlap); etc. Belongs to the PNP/MTAP family 2 of phosphorylases.; Probable purine nucleoside phosphorylase DeoD (inosine phosphorylase) (PNP) 3694860 deoD 887542 deoD Mycobacterium tuberculosis H37Rv Probable purine nucleoside phosphorylase DeoD (inosine phosphorylase) (PNP) NP_217824.1 3694054 D 83332 CDS NP_217825.1 15610444 887541 3694864..3696468 1 NC_000962.3 Rv3308, (MTV016.07), len: 534 aa. Probable pmmB,phosphomannomutase, equivalent to Q9CCL7|PMMB|ML0706 putative phospho-sugar mutase from Mycobacterium leprae (538 aa), FASTA scores: opt: 2681, E(): 1.4e-150, (76.95% identity in 538 aa overlap). Also similar to others e.g. Q9AD82|SCK13.08c from Streptomyces coelicolor (549 aa),FASTA scores: opt: 1378, E(): 8.9e-74, (46.7% identity in 529 aa overlap); Q9ZHL4|PMM (fragment so no homology at N-terminus for this one) from Haemophilus ducreyi (443 aa),FASTA scores: opt: 935, E(): 9.6e-48, (39.4% identity in 449 aa overlap); P18159|YHXB_BACSU from Bacillus subtilis (565 aa), FASTA scores: opt: 776, E(): 2.7e-38, (31.7% identity in 574 aa overlap); etc. Contains PS00710 Phosphoglucomutase and phosphomannomutase phosphoserine signature. Belongs to the phosphohexose mutases family.; Probable phosphomannomutase PmmB (phosphomannose mutase) 3696468 pmmB 887541 pmmB Mycobacterium tuberculosis H37Rv Probable phosphomannomutase PmmB (phosphomannose mutase) NP_217825.1 3694864 D 83332 CDS NP_217826.1 15610445 887944 complement(3696470..3697093) 1 NC_000962.3 Rv3309c, (MTV016.08c), len: 207 aa. Probable upp,uracil phosphoribosyltransferase, identical to P94928|UPP uracil phosphoribosyltransferase from Mycobacterium bovis (207 aa). Also similar to others e.g. P36399|UPP_STRSL from Streptococcus salivarius (209 aa), FASTA scores: opt: 658,E(): 4.7e-35, (48.3% identity in 207 aa overlap); Q9A194|UPP|SPY0392 from Streptococcus pyogenes (209 aa),FASTA scores: opt:650, E(): 1.5e-34, (47.35% identity in 207 aa overlap); Q9RE01|UPP from Lactobacillus plantarum (209 aa), FASTA scores: opt: 644, E(): 3.7e-34, (46.4% identity in 207 aa overlap); etc. Belongs to the uprtase family.; Probable uracil phosphoribosyltransferase Upp (UMP pyrophosphorylase) (uprtase) (UMP diphosphorylase) 3697093 upp 887944 upp Mycobacterium tuberculosis H37Rv Probable uracil phosphoribosyltransferase Upp (UMP pyrophosphorylase) (uprtase) (UMP diphosphorylase) NP_217826.1 3696470 R 83332 CDS NP_217827.1 15610446 887988 3697198..3698097 1 NC_000962.3 Rv3310, (MTV016.09), sapM, len: 299 aa. Secreted acid phosphatase, with N-terminal sequence beginning with ASAL..., (see Saleh and Belisle, 2000). Similar to several fungal or bacterial acid phosphatases e.g. BAB50846|MLR4110 from Rhizobium loti (Mesorhizobium loti) (292 aa), FASTA scores: opt: 460, E(): 4.8e-22, (38.65% identity in 295 aa overlap); P34724|PHOA_ASPNG from Aspergillus niger (417 aa), FASTA scores: opt: 172, E(): 0.0013, (29.1% identity in 306 aa overlap); P08540|PHOX_KLULA from Kluyveromyces lactis (Yeast) (421 aa), FASTA scores: opt: 170, E(): 0.0018, (27.8% identity in 266 aa overlap); P37274|PHOA_PENCH from Penicillium chrysogenum (412 aa),FASTA scores: opt: 163, E(): 0.0049, (29.05% identity in 303 aa overlap); etc.; Acid phosphatase (acid phosphomonoesterase) (phosphomonoesterase) (glycerophosphatase) 3698097 sapM 887988 sapM Mycobacterium tuberculosis H37Rv Acid phosphatase (acid phosphomonoesterase) (phosphomonoesterase) (glycerophosphatase) NP_217827.1 3697198 D 83332 CDS NP_217828.1 15610447 887533 3698121..3699383 1 NC_000962.3 Rv3311, (MTV016.10), len: 420 aa. Conserved protein,equivalent to Mycobacterium leprae hypothetical proteins Q9CCL8|ML0703 (423 aa), FASTA scores: opt: 2185, E(): 5.5e-120, (77.55% identity in 423 aa overlap); Q49918|L308_F2_61 (167 aa), FASTA scores: opt: 929, E(): 3.5e-47, (84.4% identity in 167 aa overlap) (similarity at C-terminus for this one); and Q49914|L308_F1_17 (166 aa),FASTA scores: opt: 900, E(): 1.7e-45, (79.0% identity in 162 aa overlap) (similarity at N-terminus for this one); Q49923|U0308N (86 aa) FASTA scores: opt: 149, E(): 0.052,(48.35% identity in 60 aa overlap); etc. Note that the Rv3311 corresponding protein in Mycobacterium leprae is similar to products of two adjacent ORFs. Also some similarity to Q9XI61|F9L1.1 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (523 aa), FASTA scores: opt: 134, E(): 1.8, (25.1% identity in 203 aa overlap). Equivalent to AAK47753 from Mycobacterium tuberculosis strain CDC1551 (431 aa) but shorter 12 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3699383 887533 Rv3311 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217828.1 3698121 D 83332 CDS NP_217829.1 15610448 887939 complement(3699404..3700330) 1 NC_000962.3 Rv3312c, (MTV016.11), len: 308 aa. Hypothetical protein, similar to various proteins (principally hypothetical unknowns or hydrolases) e.g. Q9M9P2|T17B22.7 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (326 aa), FASTA scores: opt: 261, E(): 2.6e-09,(27.55% identity in 323 aa overlap); Q9FWB6 putative alpha/beta hydrolase from Oryza sativa (Rice) (354 aa),FASTA scores: opt: 241, E(): 4.9e-08, (28.9% identity in 301 aa overlap) (note that Q9FWB6 correspond to Q9FWB5 putative alpha/beta hydrolase (353 aa) but longer 1 aa; and to Q9AUW9 hypothetical protein (332 aa) but longer 22 aa); Q9M382|F24B22.200 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (342 aa), FASTA scores: opt: 222, E(): 8e-07, (27.6% identity in 319 aa overlap); Q9HWM9|PA4152 probable hydrolase from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 176, E(): 0.00071,(29.2% identity in 209 aa overlap); Q9L3R2 hydrolase from Rhizobium leguminosarum (261 aa), FASTA scores: opt: 174,E(): 0.00071, (28.9% identity in 173 aa overlap); P49323|PRXC_STRLI|CPO|CPOL non-heme chloroperoxidase from Streptomyces lividans (275 aa), FASTA scores: opt: 172,E(): 0.001, (30.9% identity in 194 aa overlap) (similarity only at N-terminus for this one); etc. Some similarity in N-terminal part to non-heme chloroperoxidases. Also similar to O05293|Rv1191|MTCI364.03 hypothetical protein from M. tuberculosis (304 aa), FASTA scores: opt: 417, E(): 3.1e-19, (32.6% identity in 279 aa overlap) (note that Rv1191 is equivalent to AAK45485 from Mycobacterium tuberculosis strain CDC1551 but shorter 14 aa, and that AAK45485 is annoted Hydrolase, alpha/beta hydrolase family).; hypothetical protein 3700330 887939 Rv3312c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217829.1 3699404 R 83332 CDS YP_177957.1 57117088 3205113 complement(3700705..3701016) 1 NC_000962.3 Rv3312A, len: 103 aa. Secreted protein antigen,described in Corixa patent as having N-terminal sequence YYWCPGQPFDPAWGP. Equivalent to AAK47756 from Mycobacterium tuberculosis strain CDC1551 (114 aa) but shorter 11 aa.; Secreted protein antigen 3701016 3205113 Rv3312A Mycobacterium tuberculosis H37Rv Secreted protein antigen YP_177957.1 3700705 R 83332 CDS NP_217830.1 15610449 887994 complement(3701087..3702184) 1 NC_000962.3 Rv3313c, (MTV016.13), len: 365 aa. Probable add,adenosine deaminase, equivalent to Q9CCL9|add|ML0700 putative adenosine deaminase from Mycobacterium leprae (362 aa), FASTA scores: opt: 2097, E(): 1.4e-127, (88.2% identity in 356 aa overlap). Also similar to many e.g. Q9AK25|2SCK8.27 from Streptomyces coelicolor (396 aa),FASTA scores: opt: 1578, E(): 3.7e-94, (66.65% identity in 360 aa overlap); Q17747|C06G3.5 from Caenorhabditis elegans (349 aa), FASTA scores: opt: 435, E(): 1.1e-20, (29.6% identity in 348 aa overlap); P22333|ADD_ECOLI|B1623 from Escherichia coli strain K12 (333 aa), FASTA scores: opt: 380, E(): 3.7e-17, (29.4% identity in 340 aa overlap); etc. Belongs to the adenosine and AMP deaminases family.; Probable adenosine deaminase Add (adenosine aminohydrolase) 3702184 add 887994 add Mycobacterium tuberculosis H37Rv Probable adenosine deaminase Add (adenosine aminohydrolase) NP_217830.1 3701087 R 83332 CDS NP_217831.1 15610450 887929 complement(3702184..3703467) 1 NC_000962.3 Rv3314c, (MTV016.14), len: 427 aa. Probable deoA,thymidine phosporylase, highly similar to many e.g. Q9AK36|DEOA from Streptomyces coelicolor (427 aa), FASTA scores: opt: 1668, E(): 3.2e-90, (62.35% identity in 425 aa overlap); Q9CFM5|PDP from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (430 aa), FASTA scores: opt: 1031, E(): 5.5e-53, (46.45% identity in 392 aa overlap); P19971|TYPH_HUMAN|ECGF1 from Homo sapiens (Human) (482 aa),FASTA scores: opt: 957, E(): 1.3e-48, (44.45% identity in 441 aa overlap); P07650|TYPH_ECOLI|DEOA|TPP|TTG|B4382 from Escherichia coli strain K12 (440 aa), FASTA scores: opt: 847, E(): 3.2e-42, (41.55% identity in 438 aa overlap); etc. Contains PS00647 Thymidine and pyrimidine-nucleoside phosphorylases signature. Belongs to the thymidine/pyrimidine-nucleoside phosphorylases family.; Probable thymidine phosphorylase DeoA (tdrpase) (pyrimidine phosphorylase) 3703467 deoA 887929 deoA Mycobacterium tuberculosis H37Rv Probable thymidine phosphorylase DeoA (tdrpase) (pyrimidine phosphorylase) NP_217831.1 3702184 R 83332 CDS NP_217832.1 15610451 887975 complement(3703464..3703865) 1 NC_000962.3 Rv3315c, (MTV016.15c), len: 133 aa. Probable cdd,cytidine deaminase, equivalent to Q9CBD3|CDD|ML2174 cytidine deaminase from Mycobacterium leprae (134 aa),FASTA scores: opt: 516, E(): 5.8e-28, (56.8% identity in 132 aa overlap). Also highly similar to many e.g. Q9AK37|2SCK8.15 from Streptomyces coelicolor (130 aa),FASTA scores: opt: 523, E(): 1.9e-28, (60.0% identity in 130 aa overlap); Q9KD53|CDD|BH1366 from Bacillus halodurans (132 aa), FASTA scores: opt: 305, E(): 9.2e-14, (41.55% identity in 130 aa overlap); P56389|CDD_MOUSE|CDA|CDD from Mus musculus (Mouse) (146 aa), FASTA scores: opt: 287, E(): 1.6e-12, (40.3% identity in 124 aa overlap); P19079|CDD_BACSU (136 aa), FASTA scores: opt: 270, E(): 2.1e-11, (28.6% identity in 127 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family. Cofactor: zinc (by similarity).; Probable cytidine deaminase Cdd (cytidine aminohydrolase) (cytidine nucleoside deaminase) 3703865 cdd 887975 cdd Mycobacterium tuberculosis H37Rv Probable cytidine deaminase Cdd (cytidine aminohydrolase) (cytidine nucleoside deaminase) NP_217832.1 3703464 R 83332 CDS NP_217833.1 15610452 887969 3704102..3704440 1 NC_000962.3 Rv3316, (MTV016.16), len: 112 aa. Probable sdhC,cytochrome B-556 of succinate dehydrogenase SdhC subunit ,transmembrane protein, equivalent (but shorter 35 aa) to Q9CCM0|SDHC|ML0699 putative succinate dehydrogenase cytochrome B-556 subunit from Mycobacterium leprae (153 aa), FASTA scores: opt: 692, E(): 1.2e-39, (88.4% identity in 112 aa overlap). Also similar to others e.g. Q9KZ88|SC5G8.26c from Streptomyces coelicolor (126 aa),FASTA scores: opt: 484, E(): 8.3e-26, (65.65% identity in 99 aa overlap); Q9RVR8|DR0954 from Deinococcus radiodurans (118 aa), FASTA scores: opt: 195, E(): 1.7e-06, (36.8% identity in 87 aa overlap); Q9HQ63|DHSD_HALN1|SDHD|SDHC|VNG1310G from Halobacterium sp. strain NRC-1 (130 aa), FASTA scores: opt: 192, E(): 2.9e-06, (37.85% identity in 74 aa overlap); P72109|DHSD_NATPH|SDHD|SDHC from Natronomonas pharaonis (Natronobacterium pharaonis) (130 aa), FASTA scores: opt: 183, E(): 1.1e-05, (35.15% identity in 74 aa overlap); etc. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein. Belongs to the cytochrome B560 family.; Probable succinate dehydrogenase (cytochrome B-556 subunit) SdhC (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) 3704440 sdhC 887969 sdhC Mycobacterium tuberculosis H37Rv Probable succinate dehydrogenase (cytochrome B-556 subunit) SdhC (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) NP_217833.1 3704102 D 83332 CDS NP_217834.1 15610453 887845 3704437..3704871 1 NC_000962.3 Rv3317, (MTV016.17), len: 144 aa. Probable sdhD,membrane anchor of succinate dehydrogenase SdhD subunit ,equivalent (but shorter 19 aa) to Q49915|SDHD|ML0698|L308_F1_25 putative succinate dehydrogenase hydrophobic membrane anchor protein from Mycobacterium leprae (163 aa), FASTA scores: opt: 878, E(): 1.9e-51, (85.2% identity in 142 aa overlap). Also similar to others e.g. Q9KZ89|SC5G8.25c from Streptomyces coelicolor (160 aa), FASTA scores: opt: 553, E(): 6.6e-30,(58.85% identity in 141 aa overlap); Q9RVR9|DR0953 from Deinococcus radiodurans (125 aa), FASTA scores: opt: 251,E(): 5.5e-10, (37.15% identity in 113 aa overlap); O29573|DHSD_ARCFU|SDHD|AF0684 from Archaeoglobus fulgidus (117 aa), FASTA scores: opt: 160, E(): 0.00056, (25.95% identity in 108 aa overlap); etc. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur,cytochrome B-556, and an hydrophobic anchor protein.; Probable succinate dehydrogenase (hydrophobic membrane anchor subunit) SdhD (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) 3704871 sdhD 887845 sdhD Mycobacterium tuberculosis H37Rv Probable succinate dehydrogenase (hydrophobic membrane anchor subunit) SdhD (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) NP_217834.1 3704437 D 83332 CDS NP_217835.1 15610454 887639 3705000..3706772 1 NC_000962.3 Rv3318, (MTV016.18), len: 590 aa. Probable sdhA,flavoprotein of succinate dehydrogenase SdhA subunit,equivalent to Q9CCM1|SDHA|ML0697 succinate dehydrogenase flavoprotein subunit from Mycobacterium leprae (584 aa),FASTA scores: opt: 3657, E(): 1.2e-217, (92.55% identity in 590 aa overlap). Also highly similar to others e.g. Q9KZ90|DHSA from Streptomyces coelicolor (584 aa), FASTA scores: opt: 2813, E(): 1.1e-165, (70.5% identity in 586 aa overlap); Q9RVS0|DR0952 from Deinococcus radiodurans (583 aa), FASTA scores: opt: 2203, E(): 4.1e-128, (57.35% identity in 593 aa overlap); P31038|DHSA_RICPR|SDHA|RP128 from Rickettsia prowazekii (596 aa), FASTA scores: opt: 1892, E(): 5.8e-109, (50.0% identity in 588 aa overlap); P10444|DHSA_ECOLI|SDHA|B0723|Z0877|ECS0748 from Escherichia coli strains K12 and O157:H7 (588 aa), FASTA scores: opt: 1844, E(): 5.2e-106, (48.75% identity in 591 aa overlap); etc. Contains PS00504 Fumarate reductase / succinate dehydrogenase FAD-binding site. Cofactor: FAD. Similar to the flavoprotein subunits of other species succinate dehydrogenase and of fumarate reductase. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein.; Probable succinate dehydrogenase (flavoprotein subunit) SdhA (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) 3706772 sdhA 887639 sdhA Mycobacterium tuberculosis H37Rv Probable succinate dehydrogenase (flavoprotein subunit) SdhA (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) NP_217835.1 3705000 D 83332 CDS NP_217836.1 15610455 887562 3706772..3707563 1 NC_000962.3 Rv3319, (MTV016.19), len: 263 aa. Probable sdhB,iron-sulphur protein succinate dehydrogenase SdhB subunit ,equivalent to Q49916|SDHB|ML0696|L308_F1_28 succinate dehydrogenase iron-sulfur protein from Mycobacterium leprae (264 aa), FASTA scores: opt: 1678, E(): 4.7e-99, (89.8% identity in 264 aa overlap). Also highly similar to other e.g. Q9KZ91|DHSB from Streptomyces coelicolor (257 aa),FASTA scores: opt: 1125, E(): 4.6e-64, (64.1% identity in 262 aa overlap); Q9RVS1|DR0951 from Deinococcus radiodurans (264 aa), FASTA scores: opt: 1014, E(): 5e-57, (57.25% identity in 255 aa overlap); Q9PEF5|XF1073 from Xylella fastidiosa (261 aa), FASTA scores: opt: 681, E(): 5.8e-36,(45.1% identity in 244 aa overlap); P07014|DHSB_ECOLI|SDHB|B0724 from Escherichia coli strain K12 (238 aa), FASTA scores: opt: 657, E(): 1.8e-34, (43.75% identity in 240 aa overlap); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Cofactor: binds three different iron-sulfur clusters: a 2FE-2S, a 3FE-4S and a 4FE-4S. The iron-sulfur centers are similar to those of 'plant-type' 2FE-2S and 'bacterial-type' 4FE-4S ferredoxins. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein.; Probable succinate dehydrogenase (iron-sulphur protein subunit) SdhB (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) 3707563 sdhB 887562 sdhB Mycobacterium tuberculosis H37Rv Probable succinate dehydrogenase (iron-sulphur protein subunit) SdhB (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) NP_217836.1 3706772 D 83332 CDS NP_217837.1 15610456 887245 complement(3707642..3708070) 1 NC_000962.3 Rv3320c, (MTV016.20c), len: 142 aa. Possible vapC44,toxin, part of toxin-antitoxin (TA) operon with Rv0300,contains PIN domain, see Arcus et al. 2005. Similar to several others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. P95023|Rv2530c|MTCY159.26 (139 aa), FASTA scores: opt: 292, E(): 4.8e-14, (41.5% identity in 135 aa overlap); O53219|Rv2494|MTV008.50 (141 aa), FASTA scores: opt: 287, E(): 1.1e-13, (41.6% identity in 125 aa overlap); O07760|Rv0617|MTCY19H5.04c (133 aa), FASTA scores: opt: 252, E(): 3.3e-11, (37.8% identity in 127 aa overlap); etc.; Possible toxin VapC44 Contains PIN domain 3708070 vapC44 887245 vapC44 Mycobacterium tuberculosis H37Rv Possible toxin VapC44 Contains PIN domain NP_217837.1 3707642 R 83332 CDS NP_217838.1 15610457 887577 complement(3708074..3708316) 1 NC_000962.3 Rv3321c, (MTV016.21c), len: 80 aa. Possible vapB44,antitoxin, part of toxin-antitoxin (TA) operon with Rv0299,see Arcus et al. 2005. Similar to several others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. AAK48167|MT3800 DNA-binding protein (COPG family) from strain CDC1551 (74 aa), FASTA scores: opt: 142, E(): 0.0016, (48.85% identity in 43 aa overlap); AAK46916|MT2606 hypothetical 8.0 KDA protein from strain CDC1551 (74 aa),FASTA scores: opt: 139, E(): 0.0026, (37.2% identity in 78 aa overlap); O50456|Rv1241|MTV006.13 hypothetical 9.9 KDA protein from strain H37Rv (86 aa), FASTA scores: opt: 134,E(): 0.0066, (39.0% identity in 82 aa overlap); etc.; Possible antitoxin VapB44 3708316 vapB44 887577 vapB44 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB44 NP_217838.1 3708074 R 83332 CDS YP_177958.1 57117089 887520 complement(3708438..3709052) 1 NC_000962.3 Rv3322c, (MTV016.22c), len: 204 aa. Conserved hypothetical protein, showing weak similarity to proteins including several methyltransferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor (208 aa), FASTA scores: opt: 193, E(): 1e-05, (36.35% identity in 132 aa overlap); and Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa),FASTA scores: opt: 161, E(): 0.0014, (32.05% identity in 131 aa overlap); P74712|SLR1183 hypothetical 21.3 KDA protein from Synechocystis sp. strain PCC 6803 (194 aa),FASTA scores: opt: 155, E(): 0.0032, (27.35% identity in 150 aa overlap); Q9ABW8|CC0102 rRNA methyltransferase RSMB from Caulobacter crescentus (429 aa), FASTA scores: opt: 148, E(): 0.018, (31.5% identity in 162 aa overlap); etc. Also highly similar to O05796|Rv3120|MTCY164.30 hypothetical 21.8 KDA protein from Mycobacterium tuberculosis (200 aa), FASTA scores: opt: 691, E(): 1.2e-38, (56.5% identity in 200 aa overlap); and shows weak similarity to O69667|Rv3699|MTV025.047 putative methyltransferase from Mycobacterium tuberculosis (233 aa),FASTA scores: opt: 155, E(): 0.0037, (29.15% identity in 168 aa overlap).; Possible methyltransferase 3709052 887520 Rv3322c Mycobacterium tuberculosis H37Rv Possible methyltransferase YP_177958.1 3708438 R 83332 CDS YP_177959.1 57117090 887578 complement(3709049..3709714) 1 NC_000962.3 Rv3323c, (MTV016.23c), len: 221 aa. Probable moaX,MoaD-MoaE fusion protein, similar (whole or partial) to several MoaD and MoaE proteins e.g. Q9RR88|DR2607 molybdenum cofactor biosynthesis protein D/E from Deinococcus radiodurans (229 aa), FASTA scores: opt: 407,E(): 1.8e-18, (32.75% identity in 223 aa overlap); Q9K8I7|MOAE|BH3019 molybdopterin converting factor (subunit 2) from Bacillus halodurans (156 aa), FASTA scores: opt: 375, E(): 1.3e-16, (41.65% identity in 132 aa overlap); O31705|MOAE molybdopterin converting factor (subunit 2) from Bacillus subtilis (157 aa), FASTA scores: opt: 368,E(): 3.6e-16, (41.65% identity in 132 aa overlap); etc. C-terminus highly similar to O05795|MOAE_MYCTU|Rv3119|MT3201|MTCY164.29|MOAE1 putative molybdenum cofactor biosynthesis protein E from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 733, E(): 5.4e-39, (76.2% identity in 143 aa overlap); and N-terminus highly similar to O05789|MOAD1|Rv3112|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 333,E(): 3.2e-14, (65.05% identity in 83 aa overlap).; Probable MoaD-MoaE fusion protein MoaX 3709714 moaX 887578 moaX Mycobacterium tuberculosis H37Rv Probable MoaD-MoaE fusion protein MoaX YP_177959.1 3709049 R 83332 CDS NP_217841.3 448824807 887981 complement(3709715..3710248) 1 NC_000962.3 Rv3324c, (MTV016.24c), len: 177 aa. Probable moaC3,molybdopterin cofactor biosynthesis protein, highly similar to others e.g. Q9HX95|MOAC|PA3918 from Pseudomonas aeruginosa (160 aa), FASTA scores: opt: 567, E(): 7.5e-30,(58.35% identity in 156 aa overlap); Q9RKA8|MOAC from Streptomyces coelicolor (170 aa), FASTA scores: opt: 553,E(): 6.3e-29, (58.25% identity in 158 aa overlap); P30747|MOAC_ECOLI|CHLA3|B0783 from Escherichia coli strain K12 (160 aa), FASTA scores: opt: 516, E(): 1.5e-26, (55.95% identity in 159 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 734, E(): 1.3e-40, (71.8% identity in 163 aa overlap); and Rv0864|MOAC2|MTV043.57 putative molybdenum cofactor biosynthesis protein (167 aa).; Probable molybdenum cofactor biosynthesis protein C 3 MoaC3 3710248 moaC3 887981 moaC3 Mycobacterium tuberculosis H37Rv Probable molybdenum cofactor biosynthesis protein C 3 MoaC3 NP_217841.3 3709715 R 83332 CDS NP_217842.1 15610461 887314 3710433..3710759 1 NC_000962.3 Rv3325, (MTV016.25), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3325 and Rv3326,the sequence UUUUAAAG (directly upstream of Rv3326) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Belongs to the transposase family 8.; Probable transposase for insertion sequence element IS6110 (fragment) 3710759 887314 Rv3325 Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS6110 (fragment) NP_217842.1 3710433 D 83332 CDS NP_217843.2 448824808 887563 <3710708..3711694 1 NC_000962.3 Rv3326, (MTV016.26), len: 328 aa. Probable transposase for insertion element IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3325 and Rv3326,the sequence UUUUAAAG (directly upstream of Rv3326) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa).; Probable transposase 3711694 887563 Rv3326 Mycobacterium tuberculosis H37Rv Probable transposase NP_217843.2 <3710708 D 83332 CDS NP_217844.1 15610463 887965 3711749..3713461 1 NC_000962.3 Rv3327, (MTV016.27), len: 570 aa. Probable fusion protein. Indeed, N-terminal part corresponds to entire O07269 transposase of IS1547 (383 aa), and C-terminal part identical to MTCI249B.03c (210 aa). N-terminal part is identical to MTV042_7 (188 aa); C-terminal part (aa 378-570) is similar to hypothetical 20.5 kDa protein from Escherichia coli P76222|YNJA_ECOLI (182 aa), FASTA scores: opt: 292, E(): 5.3e-11, (32.6% identity in 181 aa overlap).; Probable transposase fusion protein 3713461 887965 Rv3327 Mycobacterium tuberculosis H37Rv Probable transposase fusion protein NP_217844.1 3711749 D 83332 CDS NP_217845.1 15610464 887964 complement(3713394..3714332) 1 NC_000962.3 Rv3328c, (MTV016.28c), len: 312 aa. Probable sigJ,alternative RNA polymerase sigma factor (see citations below), highly similar to many e.g. Q9K3H7|2SCG18.10c from Streptomyces coelicolor (295 aa), FASTA scores: opt: 642,E(): 7.3e-31, (42.8% identity in 292 aa overlap); Q9A3D8|CC3266 from Caulobacter crescentus (291 aa), FASTA scores: opt: 607, E(): 8.4e-29, (39.8% identity in 294 aa overlap); Q9RD74|SCF43.14c from Streptomyces coelicolor (324 aa), FASTA scores: opt: 555, E(): 1.1e-25, (41.1% identity in 297 aa overlap); etc. Similar also to U00022_20 from Mycobacterium leprae; and MTCI28_22 and MSU87307_1. Also similar to O50445|SIGI|Rv1189|MTV005.25|MTCI364.01 putative RNA polymerase sigma factor from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 426, E(): 4.2e-18, (32.65% identity in 294 aa overlap). Equivalent to AAK47774 from Mycobacterium tuberculosis strain CDC1551 (282 aa) but longer 30 aa. Contains probable helix-turn-helix motif at aa 129-150 (Score 1126, +3.02 SD). Belongs to the sigma-70 factor family, ECF subfamily.; Probable alternative RNA polymerase sigma factor (fragment) SigJ 3714332 sigJ 887964 sigJ Mycobacterium tuberculosis H37Rv Probable alternative RNA polymerase sigma factor (fragment) SigJ NP_217845.1 3713394 R 83332 CDS NP_217846.3 448824809 888028 3714392..3715708 1 NC_000962.3 Rv3329, (MTV016.29), len: 438 aa (start uncertain). Probable aminotransferase, similar to many e.g. O86744|SC6A9.12 from Streptomyces coelicolor (457 aa),FASTA scores: opt: 2120, E(): 5.1e-125, (70.1% identity in 438 aa overlap); Q9I6J2|PA0299 from Pseudomonas aeruginosa (456 aa), FASTA scores: opt: 983, E(): 5.7e-54, (38.1% identity in 425 aa overlap); Q53196|Y4UB_RHISN from Rhizobium sp. strain NGR234 plasmid sym pNGR234a (467 aa),FASTA scores: opt: 971, E(): 3.3e-53, (39.25% identity in 438 aa overlap); P33189|YHXA_BACSU from Bacillus subtilis (450 aa), FASTA scores: opt: 933, E(): 7.5e-51, (40.25% identity in 435 aa overlap); etc. Equivalent to AAK47775 from Mycobacterium tuberculosis strain CDC1551 (466 aa) but shorter 28 aa. Cofactor: pyridoxal phosphate. Could belong to class-III of pyridoxal-phosphate-dependent aminotransferases.; Probable aminotransferase 3715708 888028 Rv3329 Mycobacterium tuberculosis H37Rv Probable aminotransferase NP_217846.3 3714392 D 83332 CDS NP_217847.1 15610466 887607 3715777..3716994 1 NC_000962.3 Rv3330, (MTV016.30), len: 405 aa. Probable dacB1,D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein), equivalent to Mycobacterium leprae proteins Q9CCM2|ML0691 putative D-alanyl-D-alanine carboxypeptidase (411 aa), FASTA scores: opt: 2066, E(): 2.5e-102, (77.15% identity in 416 aa overlap); Q49917|L308_F1_36 (228 aa),FASTA scores: opt: 1241, E(): 7.9e-59, (78.9% identity in 232 aa overlap) (note that this protein corresponds to C-terminal part of the putative protein encoded by Rv3330,aa 174-405); and Q49921|PBPC (182 aa), FASTA scores: opt: 736, E(): 3.7e-32, (73.95% identity in 169 aa overlap) (note that this protein corresponds to N-terminal part of the putative protein encoded by Rv3330, aa 1-158); note L308_F1_36 (228 aa) and PBPC (182 aa) are two consecutive Mycobacterium leprae ORFs. Also similar to others e.g. Q9FC34|SC4G1.16c putative D-alanyl-D-alanine carboxypeptidase from Streptomyces coelicolor (413 aa),FASTA scores: opt: 572, E(): 3.4e-23, (33.75% identity in 382 aa overlap); P35150|DACB_BACSU penicillin-binding protein 5* precursor (D-alanyl-D-alanine carboxypeptidase) from Bacillus subtilis (382 aa), FASTA scores: opt: 422,E(): 2.8e-15, (31.3% identity in 249 aa overlap); Q9K8X5|DACB|BH2877 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein) from Bacillus halodurans (395 aa), FASTA scores: opt: 421, E(): 3.2e-15, (31.95% identity in 241 aa overlap); etc. Also similar to Mycobacterium tuberculosis Q10828|Rv2911|MTCY274.43 probable penicillin-binding protein (belongs to peptidase family S11; also known as the D-alanyl-D-alanine carboxypeptidase 1 family) (291 aa), FASTA scores: opt: 746, E(): 1.6e-32,(47.0% identity in 266 aa overlap). Has hydrophobic stretches at both N- and C-termini. Certainly membrane-bound protein. Belongs to peptidase family S11; also known as the D-alanyl-D-alanine carboxypeptidase 1 family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable penicillin-binding protein DacB1 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase) 3716994 dacB1 887607 dacB1 Mycobacterium tuberculosis H37Rv Probable penicillin-binding protein DacB1 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase) NP_217847.1 3715777 D 83332 CDS NP_217848.1 15610467 887504 3717090..3718598 1 NC_000962.3 Rv3331, (MTV016.31), len: 502 aa (start uncertain). Probable sugI, sugar-transport integral membrane protein,possibly member of major facilitator superfamily (MFS),similar to several transporters e.g. P37021|GALP_ECOLI|B2943 galactose-proton symporter (galactose transporter) from Escherichia coli strain K12 (464 aa), FASTA scores: opt: 818, E(): 1.8e-39, (31.85% identity in 446 aa overlap); P96742|YWTG metabolite-transport-related protein from Bacillus subtilis (457 aa), FASTA scores: opt: 810, E(): 5e-39, (33.2% identity in 428 aa overlap); AAG58074|GALP (alias BAB37242|ECS3819) galactose-proton symport of transport system from Escherichia coli strain O157:H7 EDL933 (464 aa), FASTA scores: opt: 810, E(): 5.1e-39, (32.2% identity in 432 aa overlap); P46333|CSBC_BACSU|SS92BR probable metabolite transport protein from Bacillus subtilis (461 aa), FASTA scores: opt: 792, E(): 5.4e-38, (33.7% identity in 442 aa overlap); etc. Equivalent to AAK47777|MT343 from Mycobacterium tuberculosis strain CDC1551 (500 aa) but with some divergence between residues 229 and 254. Contains PS00216 Sugar transport proteins signature 1 and PS00217 Sugar transport proteins signature 2. Belongs to the sugar transporter family.; Probable sugar-transport integral membrane protein SugI 3718598 sugI 887504 sugI Mycobacterium tuberculosis H37Rv Probable sugar-transport integral membrane protein SugI NP_217848.1 3717090 D 83332 CDS NP_217849.1 15610468 887518 3718595..3719746 1 NC_000962.3 Rv3332, (MTV016.32), len: 383 aa. Probable nagA,N-acetylglucosamine-6-phosphate deacetylase, similar to many e.g. Q9KXV7|SCD95A.17c putative deacetylase from Streptomyces coelicolor (381 aa), FASTA scores: opt: 1090,E(): 1.6e-55, (47.8% identity in 385 aa overlap); Q9PDB4|XF1465 N-acetylglucosamine-6-phosphate deacetylase from Xylella fastidiosa (386 aa), FASTA scores: opt: 667,E(): 3.5e-31, (38.3% identity in 394 aa overlap); Q9AAZ9|CC0443 N-acetylglucosamine-6-phosphate deacetylase from Caulobacter crescentus (378 aa), FASTA scores: opt: 661, E(): 7.5e-31, (38.9% identity in 383 aa overlap); O34450||NAGA_BACSU N-acetylglucosamine-6-phosphate deacetylase from Bacillus subtilis (396 aa), FASTA scores: opt: 571, E(): 1.2e-25, (32.45% identity in 376 aa overlap); etc. Equivalent to AAK47778 from Mycobacterium tuberculosis strain CDC1551 (346 aa) but longer 37 aa. Belongs to the NagA family.; Probable N-acetylglucosamine-6-phosphate deacetylase NagA (GlcNAc 6-P deacetylase) 3719746 nagA 887518 nagA Mycobacterium tuberculosis H37Rv Probable N-acetylglucosamine-6-phosphate deacetylase NagA (GlcNAc 6-P deacetylase) NP_217849.1 3718595 D 83332 CDS NP_217850.1 15610469 887632 complement(3719937..3720782) 1 NC_000962.3 Rv3333c, (MTV016.33c), len: 281 aa. Hypothetical unknown pro-rich protein. Equivalent to AAK47780 hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (265 aa) but longer 16 aa. Predicted to be an outer membrane protein (See Song et al., 2008).; Hypothetical proline rich protein 3720782 887632 Rv3333c Mycobacterium tuberculosis H37Rv Hypothetical proline rich protein NP_217850.1 3719937 R 83332 CDS NP_217851.1 15610470 887593 3721257..3721697 1 NC_000962.3 Rv3334, (MTV016.34), len: 146 aa. Probable transcriptional regulator, similar to many regulatory proteins (notably mercury resistance operon regulators) e.g. Q9HXV1|PA3689 probable transcriptional regulator MerR family from Pseudomonas aeruginosa (156 aa), FASTA scores: opt: 275, E(): 1.6e-11, (35.95% identity in 139 aa overlap); Q9AKR6|PBRR lead resistance operon regulator from Ralstonia metallidurans strain CH34 (plasmid pMOL30) (145 aa), FASTA scores: opt: 267, E(): 5.2e-11, (35.8% identity in 134 aa overlap); P95838|MERR mercuric resistance operon regulator from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (144 aa), FASTA scores: opt: 266, E(): 6e-11,(31.35% identity in 118 aa overlap); P22853|MERR_BACSR mercuric resistance operon regulator from Bacillus sp. strain RC607 (132 aa), FASTA scores: opt: 262, E(): 1e-10,(34.6% identity in 130 aa overlap); etc. Contains probable helix-turn-helix motif at aa 1-22 (Score 1478, +4.22 SD). Seems to belong to the MerR family of transcriptional regulators.; Probable transcriptional regulatory protein (probably MerR-family) 3721697 887593 Rv3334 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein (probably MerR-family) NP_217851.1 3721257 D 83332 CDS NP_217852.1 15610471 888029 complement(3721731..3722600) 1 NC_000962.3 Rv3335c, (MTV016.35c), len: 289 aa. Probable conserved integral membrane protein, equivalent to Q49909|ML0687 putative membrane protein U0308AA from Mycobacterium leprae (313 aa), FASTA scores: opt: 1299,E(): 8.9e-75, (68.75% identity in 288 aa overlap). Also similar to other hypothetical bacterial proteins e.g. BAB37825|ECS4402 from Escherichia coli strain O157:H7 (alias P37642|YHJD_ECOLI|B3522 strain K12) (337 aa), FASTA scores: opt: 591, E(): 4.2e-30, (35.15% identity in 273 aa overlap); P45417|YHJD_ERWCH from Erwinia chrysanthemi (328 aa), FASTA scores: opt: 500, E(): 2.2e-24, (34.9% identity in 275 aa overlap); Q9KZA0|SC5G8.14 putative integral membrane protein from Streptomyces coelicolor (321 aa),FASTA scores: opt: 321, E(): 4.3e-13, (27.3% identity in 271 aa overlap); etc.; Probable conserved integral membrane protein 3722600 888029 Rv3335c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217852.1 3721731 R 83332 CDS NP_217853.1 15610472 887559 complement(3722621..3723631) 1 NC_000962.3 Rv3336c, (MTV016.36c), len: 336 aa. Probable trpS,tryptophanyl-tRNA synthetase, equivalent to Q49901|SYW_MYCLE|TRPS|ML0686|L308_C1_147 tryptophanyl-tRNA synthetase from Mycobacterium leprae (343 aa), FASTA scores: opt: 1859, E(): 4.8e-107, (83.75% identity in 339 aa overlap). Also similar to many e.g. Q9KZA7|TRPS2 from Streptomyces coelicolor (339 aa), FASTA scores: opt: 1359,E(): 2.6e-76, (60.3% identity in 335 aa overlap); Q9EYY6|TRPS from Klebsiella aerogenes (334 aa), FASTA scores: opt: 1077, E(): 5.5e-59, (52.15% identity in 328 aa overlap); P00954|SYW_ECOLI|TRPS|B3384 from Escherichia coli strain K12 (334 aa), FASTA scores: opt: 1074, E(): 8.3e-59,(51.85% identity in 328 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family.; Probable tryptophanyl-tRNA synthetase TrpS (tryptophan--tRNA ligase) (TRPRS) (tryptophan translase) 3723631 trpS 887559 trpS Mycobacterium tuberculosis H37Rv Probable tryptophanyl-tRNA synthetase TrpS (tryptophan--tRNA ligase) (TRPRS) (tryptophan translase) NP_217853.1 3722621 R 83332 CDS NP_217854.2 57117091 887556 3723656..3724042 1 NC_000962.3 Rv3337, (MTV016.37), len: 128 aa. Conserved hypothetical protein, equivalent to N-terminus of Q49926|ML0685 TPEA (putative hydrolase) from Mycobacterium leprae (303 aa), FASTA scores: opt: 362, E(): 5.7e-17,(74.3% identity in 70 aa overlap). Also weak similarity in N-terminus to Q98JT7|BAB49078|MLR1789 probable epoxide hydrolase from Rhizobium loti (Mesorhizobium loti) (300 aa), FASTA scores: opt: 122, E(): 0.74, (31.95% identity in 97 aa overlap). Homology suggests this ORF should be in frame with the following ORF MTV016.38 but no sequence error could be found. Short distance to start of trpS suggests region may not be protein-coding. C-terminus extended since first submission (+47 aa).; hypothetical protein 3724042 887556 Rv3337 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217854.2 3723656 D 83332 CDS NP_217855.1 15610474 888020 3723904..3724548 1 NC_000962.3 Rv3338, (MTV016.38), len: 214 aa. Hypothetical protein, equivalent to C-termini of Q49926|ML0685 TPEA (putative hydrolase) from Mycobacterium leprae (303 aa),FASTA scores: opt: 984, E(): 2.6e-56, (65.4% identity in 214 aa overlap); and O32873|MLCB1779.02 hypothetical 31.8 KDA protein (similar to alpha/beta hydrolase fold) from Mycobacterium leprae (292 aa), FASTA scores: opt: 984, E(): 2.5e-56, (65.4% identity in 214 aa overlap). Also similar to C-termini of several hypothetical proteins (generally hydrolases) e.g. Q9K3H6|2SCG18.11 putative hydrolase from Streptomyces coelicolor (316 aa), FASTA scores: opt: 213,E(): 1.4e-06, (29.75% identity in 185 aa overlap). Homology suggests that this ORF should be in frame with the previous ORF MTV016.37 but no sequence error could be found.; hypothetical protein 3724548 888020 Rv3338 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217855.1 3723904 D 83332 CDS NP_217856.1 15610475 888013 complement(3724615..3725844) 1 NC_000962.3 Rv3339c, (MTV016.39c), len: 409 aa. Probable icd1,isocitrate dehydrogenase NADP-dependent, highly similar to many e.g. Q9A5C8|CC2522 from Caulobacter crescentus (403 aa), FASTA scores: opt: 1972, E(): 4.6e-115, (72.45% identity in 403 aa overlap); AAF73472|ICD from Rhizobium meliloti (404 aa), FASTA scores: opt: 1968, E(): 8.1e-115,(73.2% identity in 403 aa overlap); P50215|IDH_SPHYA from Sphingomonas yanoikuyae (406 aa), FASTA scores: opt: 1964,E(): 1.4e-114, (71.45% identity in 403 aa overlap); etc. Contains PS00470 Isocitrate and isopropylmalate dehydrogenases signature. Belongs to the isocitrate and isopropylmalate dehydrogenases family. Note that in H37Rv,Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2.; Probable isocitrate dehydrogenase [NADP] Icd1 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) 3725844 icd1 888013 icd1 Mycobacterium tuberculosis H37Rv Probable isocitrate dehydrogenase [NADP] Icd1 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) NP_217856.1 3724615 R 83332 CDS NP_217857.1 15610476 888037 3726127..3727476 1 NC_000962.3 Rv3340, (MTV016.40), len: 449 aa. Probable metC,O-acetyl-L-homoserine sulfhydrylase, highly similar to many e.g. Q9K9P2|BH2603 O-acetylhomoserine sulfhydrylase from Bacillus halodurans (430 aa), FASTA scores: opt: 1716, E(): 3.3e-97, (60.45% identity in 425 aa overlap); Q9HUE4|METY|PA5025 homocysteine synthase from Pseudomonas aeruginosa (425 aa), FASTA scores: opt: 1517, E(): 4.4e-85,(56.95% identity in 425 aa overlap); Q9WZY4|TM0882 O-acetylhomoserine sulfhydrylase from Thermotoga maritima (430 aa), FASTA scores: opt: 1488, E(): 2.6e-83, (55.75% identity in 418 aa overlap); BAB54344|MLR8465 O-acetylhomoserine sulfhydrylase from Rhizobium loti (Mesorhizobium loti) (426 aa), FASTA scores: opt: 1445,E(): 1.1e-80, (53.2% identity in 419 aa overlap); P50125|CYSD_EMENI O-acetylhomoserine (thiol)-lyase from Emericella nidulans (Aspergillus nidulans) (437 aa), FASTA scores: opt: 1442, E(): 1.7e-80, (53.7% identity in 430 aa overlap); etc. Contains PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. Cofactor: pyridoxal phosphate. Belongs to the trans-sulfuration enzymes family.; Probable O-acetylhomoserine sulfhydrylase MetC (homocysteine synthase) (O-acetylhomoserine (thiol)-lyase) (OAH sulfhydrylase) (O-acetyl-L-homoserine sulfhydrylase) 3727476 metC 888037 metC Mycobacterium tuberculosis H37Rv Probable O-acetylhomoserine sulfhydrylase MetC (homocysteine synthase) (O-acetylhomoserine (thiol)-lyase) (OAH sulfhydrylase) (O-acetyl-L-homoserine sulfhydrylase) NP_217857.1 3726127 D 83332 CDS NP_217858.1 15610477 888030 3727488..3728627 1 NC_000962.3 Rv3341, (MTV016.41), len: 379 aa. Probable metA,homoserine o-acetyltransferase (see citation below),equivalent to O32874|METX_MYCLE|meta|ML0682|MLCB1779.11 homoserine O-acetyltransferase from Mycobacterium leprae (382 aa), FASTA scores: opt: 2263, E(): 9.2e-129, (85.0% identity in 380 aa overlap). Also highly similar to many e.g. O68640|METX_CORGL|meta from Corynebacterium glutamicum (Brevibacterium flavum) (379 aa), FASTA scores: opt: 1135,E(): 5.9e-61, (48.5% identity in 371 aa overlap); Q9AAS1|CC0525 from Caulobacter crescentus (382 aa), FASTA scores: opt: 860, E(): 2e-44, (40.5% identity in 363 aa overlap); P94891|METX_LEPME from Leptospira meyeri (379 aa), FASTA scores: opt: 787, E(): 4.9e-40, (38.2% identity in 385 aa overlap); etc. Belongs to the ab hydrolase family, HTA subfamily.; Probable homoserine O-acetyltransferase MetA (homoserine O-trans-acetylase) (homoserine transacetylase) (HTA) 3728627 metA 888030 metA Mycobacterium tuberculosis H37Rv Probable homoserine O-acetyltransferase MetA (homoserine O-trans-acetylase) (homoserine transacetylase) (HTA) NP_217858.1 3727488 D 83332 CDS NP_217859.1 15610478 888046 3728624..3729355 1 NC_000962.3 Rv3342, (MTV016.42), len: 243 aa. Possible methyltransferase, similar to various proteins e.g. Q9I5X8|PA0558 hypothetical protein from Pseudomonas aeruginosa (255 aa), FASTA scores: opt: 496, E(): 4.4e-24,(39.85% identity in 236 aa overlap); Q9XBC9|CZA382.22c putative rRNA methylase from Amycolatopsis orientalis (259 aa), FASTA scores: opt: 473, E(): 1.2e-22, (42.45% identity in 245 aa overlap); Q9UTA8|SPAC25B8.10 putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (256 aa), FASTA scores: opt: 470, E(): 1.9e-22,(35.7% identity in 238 aa overlap); and Q9UTA9|SPAC25B8.09 putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (251 aa), FASTA scores: opt: 418, E(): 3.4e-19, (31.2% identity in 237 aa overlap); etc. Start uncertain. Belongs to the methyltransferase superfamily.; Possible methyltransferase (methylase) 3729355 888046 Rv3342 Mycobacterium tuberculosis H37Rv Possible methyltransferase (methylase) NP_217859.1 3728624 D 83332 CDS YP_177960.1 57117092 888033 complement(3729364..3736935) 1 NC_000962.3 Rv3343c, (MTV016.43c), len: 2523 aa. PPE54, Member of the Mycobacterium tuberculosis PPE family, MPTR subgroup of Gly-, Asn-rich proteins. Most similar to O50379|Rv3350c|MTV004.07c|MTV004_5 from Mycobacterium tuberculosis strain H37Rv (3716 aa), FASTA scores: opt: 4672, E(): 4e-211, (44.2% identity in 3174 aa overlap); and also similar to MTV004_3, MTCY63_9, MTY13E10_17,MTY13E10_16, MTCY180_1, MTV050_1, MTCY3C7_23, MTV014_3,MTCY63_10; etc.; PPE family protein PPE54 3736935 PPE54 888033 PPE54 Mycobacterium tuberculosis H37Rv PPE family protein PPE54 YP_177960.1 3729364 R 83332 CDS YP_177961.1 57117093 888115 complement(3736984..>3738438) 1 NC_000962.3 Rv3344c, (MTV016.44c), len: 484 aa. PE_PGRS49,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-, ala-rich proteins (see Brennan and Delogu, 2002). Appears to be a gene fragment, should be in-frame with following ORF, MTV016.45c, frameshift required around 49595 but could not be found on checking BAC and cosmid clones. Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O53557|Rv3512|MTV023.19 (1079 aa), FASTA scores: opt: 1595,E(): 1.8e-54, (52.0% identity in 544 aa overlap).; PE-PGRS family protein PE_PGRS49 >3738438 PE_PGRS49 888115 PE_PGRS49 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS49 YP_177961.1 3736984 R 83332 CDS YP_177962.1 57117094 888114 complement(3738158..3742774) 1 NC_000962.3 Rv3345c, (MTV004.01c-MTV016.45c), len: 1538 aa. PE_PGRS50, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002). Similar to AAK47791 from strain CDC1551 but with some big gaps (after residues 501 and 1419; and for AAK47791 after residue 991). Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551.; PE-PGRS family protein PE_PGRS50 3742774 PE_PGRS50 888114 PE_PGRS50 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS50 YP_177962.1 3738158 R 83332 CDS NP_217863.1 15610482 888010 complement(3743198..3743455) 1 NC_000962.3 Rv3346c, (MTV004.02c), len: 85 aa. Conserved transmembrane protein, highly similar to mycobacterium hypothetical proteins O50384|Rv3355c|MTV004.12c from strain H37Rv (97 aa), FASTA scores: opt: 413, E(): 4.6e-23,(85.55% identity in 97 aa overlap); O32878|MLCB1779.16c|ML0675 from Mycobacterium leprae (91 aa), FASTA scores: opt: 349, E(): 1.7e-18, (67.35% identity in 95 aa overlap). Contains possible membrane spanning regions.; Conserved transmembrane protein 3743455 888010 Rv3346c Mycobacterium tuberculosis H37Rv Conserved transmembrane protein NP_217863.1 3743198 R 83332 CDS YP_177963.1 57117095 888120 complement(3743711..3753184) 1 NC_000962.3 Rv3347c, (MTV004.03c), len: 3157 aa. PPE55, Member of the Mycobacterium tuberculosis PPE family, Gly-, Ala-,Asn-rich protein. Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551, e.g. O50379|Rv3350c|MTV004.07c (3716 aa), FASTA scores: opt: 6497, E(): 0, (61.65% identity in 3756 aa overlap); and other upstream ORFs MTV004_5, MTY13E10_15, MTCY28_16,MTCY63_9, MTY13E10_17, MTCY180_1; etc. Predicted possible vaccine candidate (See Zvi et al., 2008).; PPE family protein PPE55 3753184 PPE55 888120 PPE55 Mycobacterium tuberculosis H37Rv PPE family protein PPE55 YP_177963.1 3743711 R 83332 CDS NP_217865.1 15610484 888110 3753765..3754256 1 NC_000962.3 Rv3348, (MTV004.04), len: 163 aa. Probable transposase, partially similar to several insertion elements e.g. P19834|YI11_STRCL insertion element IS116 hypothetical 44.8 KDA protein (similar to IS900 of Mycobacterium paratuberculosis) from Streptomyces clavuligerus (399 aa), FASTA scores: opt: 146, E(): 0.016,(29.1% identity in 158 aa overlap).; Probable transposase 3754256 888110 Rv3348 Mycobacterium tuberculosis H37Rv Probable transposase NP_217865.1 3753765 D 83332 CDS NP_217866.1 15610485 888126 complement(3754293..3755033) 1 NC_000962.3 Rv3349c, (MTV004.05c), len: 246 aa. Probable transposase pseudogene fragment, similar to part of Q50911|U10634 IS204 putative transposase from nocardia asteroides (377 aa), FASTA scores: opt: 288, E(): 8.3e-11,(48.5% identity in 97 aa overlap); and others.; Probable transposase 3755033 888126 Rv3349c Mycobacterium tuberculosis H37Rv Probable transposase NP_217866.1 3754293 R 83332 CDS YP_177964.1 57117096 888113 complement(3755952..3767102) 1 NC_000962.3 Rv3350c, (MTV004.07c), len: 3716 aa. PPE56, Member of the Mycobacterium tuberculosis PPE family of Gly-, Ala-,Asn-rich proteins, similar to many Mycobacterium tuberculosis proteins from strains H37Rv and CDC1551, e.g. O50378|Rv3347c|MTV004.03c (3157 aa), FASTA scores: opt: 6497, E(): 0, (61.65% identity in 3756 aa overlap); MTCY28_16, MTV050_2, MTY13E10_17, MTCY63_10, MTCY180_1,MTCY63_9, MTV050_1, MTV014_3, MTY13E10_15; etc.; PPE family protein PPE56 3767102 PPE56 888113 PPE56 Mycobacterium tuberculosis H37Rv PPE family protein PPE56 YP_177964.1 3755952 R 83332 CDS NP_217868.1 15610487 888109 complement(3767346..3768140) 1 NC_000962.3 Rv3351c, (MTV004.08c), len: 264 aa. Hypothetical protein, highly similar to C-terminal region (aa 292-479) of O53608|Rv0063|MTV030.06 oxidoreductase from Mycobacterium tuberculosis (479 aa), FASTA scores: opt: 699, E(): 1.7e-36, (54.75% identity in 190 aa overlap). Shows some similarity to Q9KYD6|SCD72A.20 putative lipoprotein (fragment) from Streptomyces coelicolor (403 aa), FASTA scores: opt: 192, E(): 9.1e-05, (27.9% identity in 154 aa overlap); and P71091|YGAK hypothetical 54.4 KDA protein from Bacillus subtilis (480 aa), FASTA scores: opt: 174, E(): 0.0014, (26.5% identity in 166 aa overlap). Note that the two upstream ORFs Rv3352c and Rv3353c also show similarity to Rv0063 (MTV030_7). Sequence was checked but no errors found. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 3768140 888109 Rv3351c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217868.1 3767346 R 83332 CDS NP_217869.1 15610488 888104 complement(3768222..3768593) 1 NC_000962.3 Rv3352c, (MTV004.09c), len: 123 aa. Possible oxidoreductase, similar to part of several oxidoreductases (and hypothetical proteins) from diverse organisms e.g. Q9KYD6|SCD72A.20 putative lipoprotein (fragment) from Streptomyces coelicolor (403 aa), FASTA scores: opt: 348,E(): 7.9e-15, (51.0% identity in 102 aa overlap); BAB53081|MLR6875 probable oxidoreductase from Rhizobium loti (Mesorhizobium loti) (479 aa), FASTA scores: opt: 262,E(): 2.3e-09, (53.85% identity in 78 aa overlap); O94206|OX1 oxidoreductase from Claviceps purpurea (Ergot fungus) (483 aa), FASTA scores: opt: 245, E(): 2.7e-08,(42.6% identity in 115 aa overlap); Q9KHK2|ENCM putative FAD-dependent oxygenase ENCM from Streptomyces maritimus (464 aa), FASTA scores: opt: 238, E(): 7.2e-08, (43.95% identity in 91 aa overlap); etc. Also highly similar to part of O53608|Rv0063|MTV030.06 oxidoreductase (479 aa),FASTA scores: opt: 599, E(): 1.6e-30, (71.55% identity in 123 aa overlap); and to other Mycobacterium tuberculosis proteins e.g. Rv3353c and Rv3351c. All show similarity to a family of oxidoreductases in Mycobacterium tuberculosis,suggesting that frameshift mutations may have occurred. Sequence has been checked but no errors were found.; Possible oxidoreductase 3768593 888104 Rv3352c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_217869.1 3768222 R 83332 CDS NP_217870.2 57117097 888095 complement(3768736..3768996) 1 NC_000962.3 Rv3353c, (MTV004.10c), len: 86 aa. Hypothetical protein, showing some similarity to Q9X5Q4|MITR MITR protein from Streptomyces lavendulae (514 aa), FASTA scores: opt: 134, E(): 0.09, (29.5% identity in 78 aa overlap); and weak to Q49720|B1549_C3_218 from Mycobacterium leprae (222 aa), FASTA scores: opt: 99, E(): 8.8, (32.9% identity in 76 aa overlap). But highly similar to N-terminal part of O53608|Rv0063|MTV030.06 oxidoreductase from Mycobacterium tuberculosis (479 aa),FASTA scores: opt: 305, E(): 4.9e-13, (52.9% identity in 87 aa overlap); and some similarity can be found with Rv3352c and Rv3351c. All show similarity to a family of oxidoreductases in Mycobacterium tuberculosis, suggesting that frameshift mutations may have occurred. Sequence has been checked but no errors were found. Start changed since original submission.; hypothetical protein 3768996 888095 Rv3353c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217870.2 3768736 R 83332 CDS NP_217871.1 15610490 887643 3769111..3769500 1 NC_000962.3 Rv3354, (MTV004.11), len: 129 aa. Conserved hypothetical protein, equivalent (but shorter 29 aa) to Q9CCM4|ML0676 hypothetical protein from Mycobacterium leprae (158 aa), FASTA scores: opt: 467, E(): 3.3e-21,(55.9% identity in 127 aa overlaps). Highly similar to O33192|LPRJ|Rv1690|MTCI125.12 hypothetical protein from Mycobacterium tuberculosis (127 aa), FASTA scores: opt: 329, E(): 4.7e-13, (46.95% identity in 115 aa overlap); and also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O07222|Rv1810|MTCY16F9.04c (118 aa), FASTA scores: opt: 195, E(): 4.2e-05, (37.15% identity in 113 aa overlap); MTCI125_11, MTCY16F9_4, MTV049_25. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3769500 887643 Rv3354 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217871.1 3769111 D 83332 CDS NP_217872.1 15610491 887660 complement(3769514..3769807) 1 NC_000962.3 Rv3355c, (MTV004.12c), len: 97 aa. Probable integral membrane protein, equivalent to O32878|MLCB1779.16c|ML0675 hypothetical 9.6 KDA protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 439, E(): 3.9e-23, (78.9% identity in 90 aa overlap). Identical, but with a gap, to O50377|Rv3346c|MTV004.02c hypothetical 8.9 KDA protein from Mycobacterium tuberculosis (85 aa), FASTA scores: opt: 413,E(): 2.1e-21, (85.55% identity in 97 aa overlap). Also some similarity to other proteins e.g. Q9K3J5|SC2A6.10 putative integral membrane protein from Streptomyces coelicolor (178 aa), FASTA scores: opt: 147, E(): 0.003, (31.25% identity in 80 aa overlap).; Probable integral membrane protein 3769807 887660 Rv3355c Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_217872.1 3769514 R 83332 CDS NP_217873.1 15610492 888145 complement(3769804..3770649) 1 NC_000962.3 Rv3356c, (MTV004.13c), len: 281 aa. Probable folD,bifunctional enzyme include methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase ,equivalent to O32879|fold|ML0674 methylenetetrahydrofolate dehydrogenase (putative methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase) from Mycobacterium leprae (282 aa), FASTA scores: opt: 1624,E(): 1.2e-93, (86.45% identity in 281 aa overlap). Also similar to many others e.g. Q9K3J6|fold from Streptomyces coelicolor (284 aa), FASTA scores: opt: 1223, E(): 9.5e-69,(66.65% identity in 279 aa overlap); Q9K966|fold from Bacillus halodurans (279 aa), FASTA scores: opt: 886, E(): 7.7e-48, (47.15% identity in 280 aa overlap); P54382|FOLD_BACSU from Bacillus subtilis (283 aa), FASTA scores: opt: 820, E(): 9.7e-44, (45.7% identity in 280 aa overlap); P51696|FOLD_PHOPO from Photobacterium phosphoreum (285 aa), FASTA scores: opt: 778, E(): 4e-41, (44.9% identity in 283 aa overlap); P24186|FOLD_ECOLI|ads|B0529 from Escherichia coli (287 aa), FASTA scores: opt: 741,E(): 0,44.4, (44.4% identity in 277 aa overlap); etc. Also highly similar to MLCB1779_9 from Mycobacterium leprae cosmid B1779 (282 aa) (86.5% identity in 281 aa overlap). Similar to other dehydrogenase/cyclohydrolase enzymes or domains.; Probable bifunctional protein FolD: methylenetetrahydrofolate dehydrogenase + methenyltetrahydrofolate cyclohydrolase 3770649 folD 888145 folD Mycobacterium tuberculosis H37Rv Probable bifunctional protein FolD: methylenetetrahydrofolate dehydrogenase + methenyltetrahydrofolate cyclohydrolase NP_217873.1 3769804 R 83332 CDS NP_217874.1 15610493 888135 3770773..3771048 1 NC_000962.3 Rv3357, (MTV004.14), len: 91 aa. RelJ, antitoxin,part of toxin-antitoxin (TA) operon with Rv3358 (See Cherny et al., 2004; Pandey and Gerdes, 2005), highly similar to other hypothetical proteins e.g. Q9Z4V7|YU1E_STRCO (alias CAC37261|SCBAC17D6.02) ORFU1E (belongs to the PHD/YEFM family) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147|YEFM_ECOLI|B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570|SAV2408 from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 161, E(): 8.8e-05, (39.95% identity in 77 aa overlap); Q9Z5W8 putative PHD protein from Francisella novicid (85 aa), FASTA scores: opt: 143, E(): 0.0016,(28.9% identity in 83 aa overlap); etc. Also similar to Rv1247c|MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). Seems to belong to the PHD/YEFM family.; Antitoxin RelJ 3771048 relJ 888135 relJ Mycobacterium tuberculosis H37Rv Antitoxin RelJ NP_217874.1 3770773 D 83332 CDS NP_217875.1 15610494 888139 3771045..3771302 1 NC_000962.3 Rv3358, (MTV004.15), len: 85 aa. RelK, toxin, part of toxin-antitoxin (TA) operon with Rv3357 (See Cherny et al., 2004; Pandey and Gerdes, 2005), highly similar to other hypohetical proteins e.g. Q9Z4V8|SCBAC17D6.03 from Streptomyces coelicolor (84 aa), FASTA scores: opt: 393,E(): 1.1e-21, (59.75% identity in 82 aa overlap); P56605|YOEB_ECOLI from Escherichia coli (84 aa), FASTA scores: opt: 305, E(): 2.2e-15, (49.35% identity in 77 aa overlap); Q9Z5W7 putative doc protein from Francisella novicida (68 aa), FASTA scores: opt: 253, E(): 9.6e-12,(51.6% identity in 62 aa overlap); BAB58569|SAV2407 from Staphylococcus aureus subsp. aureus Mu50 (88 aa), FASTA scores: opt: 250, E(): 2e-11, (40.5% identity in 84 aa overlap); etc.; Toxin RelK 3771302 relK 888139 relK Mycobacterium tuberculosis H37Rv Toxin RelK NP_217875.1 3771045 D 83332 CDS NP_217876.1 15610495 887668 3771344..3772534 1 NC_000962.3 Rv3359, (MTV004.16), len: 396 aa. Possible oxidoreductase, similar to N-terminal part of various proteins (hypothetical unknowns or oxidoreductases) e.g. Q9ZB94 hypothetical 69.3 KDA protein from Rhodococcus erythropolis (649 aa), FASTA scores: opt: 509, E(): 3e-24,(30.0% identity in 380 aa overlap); O29991|AF0248 NADH-dependent flavin oxidoreductase from Archaeoglobus fulgidus (378 aa), FASTA scores: opt: 478, E(): 1.6e-22,(32.45% identity in 379 aa overlap); Q9HUH9|PA4986 probable oxidoreductase from Pseudomonas aeruginosa (648 aa), FASTA scores: opt: 412, E(): 3.3e-18, (30.45% identity in 384 aa overlap); Q9KCT8|BH1481 NADH oxidase from Bacillus halodurans (338 aa), FASTA scores: opt: 404, E(): 6.1e-18,(30.2% identity in 275 aa overlap); etc. Some weak similarity to Mycobacterium leprae MLCB1779_10.; Possible oxidoreductase 3772534 887668 Rv3359 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_217876.1 3771344 D 83332 CDS NP_217877.1 15610496 888136 3772651..3773019 1 NC_000962.3 Rv3360, (MTV004.17), len: 122 aa. Hypothetical protein, highly similar to the N-terminus of O65934|Rv1747|MTCY28.10|MTCY04C12.31 probable ABC-transporter ATP-binding protein from Mycobacterium tuberculosis (865 aa), FASTA scores: opt: 480, E(): 4.7e-25, (61.0% identity in 118 aa overlap); and some similarity with the N-terminus of P96214|Rv3863|MTCY01A6.05c hypothetical 41.1 KDA protein from Mycobacterium tuberculosis (392 aa), FASTA scores: opt: 138, E(): 0.033, (31.95% identity in 97 aa overlap). Some weak similarity with the N-terminus of other hypothetical proteins e.g. P73823|CYAA|SLR1991 adenylate cyclase from Synechocystis sp. strain PCC 6803 (337 aa),FASTA scores: opt: 127, E(): 0.16, (28.55% identity in 112 aa overlap).; hypothetical protein 3773019 888136 Rv3360 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217877.1 3772651 D 83332 CDS NP_217878.1 15610497 888130 complement(3773016..3773567) 1 NC_000962.3 Rv3361c, (MTV004.18c), len: 183 aa. Conserved protein, with some similarity to various proteins e.g. P74221|YB52_SYNY3|SLR1152 hypothetical 36.2 KDA protein SLR (contains 5 pentapeptide repeat domains) from Synechocystis sp. strain PCC 6803 (331 aa), FASTA scores: opt: 252, E(): 3.9e-10, (30.55% identity in 167 aa overlap); Q9SE95 FH protein interacting protein FIP2 from Arabidopsis thaliana (Mouse-ear cress) (298 aa), FASTA scores: opt: 207, E(): 4.4e-07, (30.35% identity in 168 aa overlap); Q9A735|CC1891 pentapeptide repeat family protein from Caulobacter crescentus (250 aa), FASTA scores: opt: 181, E(): 2.3e-05,(24.05% identity in 187 aa overlap); etc.; hypothetical protein 3773567 888130 Rv3361c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217878.1 3773016 R 83332 CDS NP_217879.1 15610498 888088 complement(3773574..3774155) 1 NC_000962.3 Rv3362c, (MTV004.19c), len: 193 aa. Probable ATP/GTP-binding protein, similar to others from Streptomyces coelicolor e.g. O86519|SC1C2.18c (174 aa),FASTA scores: opt: 731, E(): 9.8e-41, (66.85% identity in 169 aa overlap); Q9XAE1|SC6G9.41c (191 aa), FASTA scores: opt: 730, E(): 1.2e-40, (63.55% identity in 173 aa overlap); Q9L235|SC1A2.06 (184 aa), FASTA scores: opt: 650,E(): 1.9e-35, (55.95% identity in 177 aa overlap); Q9RJ74|SCI41.10c (176 aa), FASTA scores: opt: 618, E(): 2.3e-33, (55.9% identity in 161 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable ATP/GTP-binding protein 3774155 888088 Rv3362c Mycobacterium tuberculosis H37Rv Probable ATP/GTP-binding protein NP_217879.1 3773574 R 83332 CDS NP_217880.1 15610499 888090 complement(3774136..3774504) 1 NC_000962.3 Rv3363c, (MTV004.20c), len: 122 aa. Conserved hypothetical protein, similar to others from Streptomyces coelicolor e.g. O86523|SC1C2.23c (132 aa), FASTA scores: opt: 236, E(): 9e-09, (38.5% identity in 122 aa overlap); O86520|SC1C2.19c (190 aa), FASTA scores: opt: 231, E(): 2.7e-08, (41.0% identity in 122 aa overlap); Q9X834|SC9B1.14c (119 aa), FASTA scores: opt: 188, E(): 1.1e-05, (37.5% identity in 120 aa overlap); Q9ADJ4|SCBAC14E8.05 (113 aa), FASTA scores: opt: 167, E(): 0.00025, (33.05% identity in 109 aa overlap); etc.; hypothetical protein 3774504 888090 Rv3363c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217880.1 3774136 R 83332 CDS NP_217881.1 15610500 888085 complement(3774482..3774874) 1 NC_000962.3 Rv3364c, (MTV004.21c), len: 130 aa. Conserved protein, highly similar to others from Streptomyces coelicolor e.g. O86524|SC1C2.24c (137 aa), FASTA scores: opt: 466, E(): 1.3e-22, (58.6% identity in 116 aa overlap); O86521|SC1C2.20c (140 aa), FASTA scores: opt: 445, E(): 2.7e-21, (56.9% identity in 116 aa overlap); Q9KZI6|SCG8A.13c (145 aa), FASTA scores: opt: 341, E(): 9.5e-15, (51.3% identity in 113 aa overlap); etc.; hypothetical protein 3774874 888085 Rv3364c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217881.1 3774482 R 83332 CDS NP_217882.1 15610501 887652 complement(3774871..3777501) 1 NC_000962.3 Rv3365c, (MTV004.22c), len: 876 aa. Conserved protein, similar to various proteins from Streptomyces coelicolor e.g. O86525|SC1C2.25c hypothetical 139.7 KDA protein (similar to other prokaryotic sensory transduction histidine kinases) (1329 aa), FASTA scores: opt: 879, E(): 5.4e-32, (29.9% identity in 924 aa overlap) (similarity in N-terminal part for this one); O86522|SC1C2.21c hypothetical 119.9 KDA protein (similar to other prokaryotic sensory transduction histidine kinases) (1111 aa), FASTA scores: opt: 855, E(): 5.6e-31, (28.9% identity in 892 aa overlap) (similarity in N-terminal part for this one); Q9KZI5|SCG8A.14c putative membrane protein (862 aa),FASTA scores: opt: 791, E(): 3.3e-28, (30.8% identity in 828 aa overlap); Q9KZN0|SC1A8A.22c (943 aa), FASTA scores: opt: 660, E(): 2.5e-22, (27.65% identity in 893 aa overlap); etc. Similar in part to two consecutive Mycobacterium leprae hypothetical ORFs, probably representing a pseudogene: O07701|MLCL383.27 (118 aa),FASTA scores: opt: 430, E(): 1e-12, (58.25% identity in 115 aa overlap); and O07700|MLCL383.26 (111 aa), FASTA scores: opt: 271, E(): 1.3e-05, (50.4% identity in 121 aa overlap). Contains PS00142 Neutral zinc metallopeptidases,zinc-binding region signature.; hypothetical protein 3777501 887652 Rv3365c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217882.1 3774871 R 83332 CDS NP_217883.1 15610502 887648 3777737..3778201 1 NC_000962.3 Rv3366, (MTV004.23), len: 154 aa. Probable spoU,tRNA/rRNA methylase, equivalent to Q9CCU7|ML0419 putative tRNA/rRNA methyltransferase from Mycobacterium leprae (158 aa), FASTA scores: opt: 861, E(): 1.2e-50, (83.75% identity in 154 aa overlap); and O07698|MLCL383.24c rRNA methylase from Mycobacterium leprae (169 aa), FASTA scores: opt: 861,E(): 1.3e-50, (83.75% identity in 154 aa overlap). Also highly similar to many members of the spoU family of rRNA methylases e.g. Q9K199|NMB0268 RNA methyltransferase (TRMH family) from Neisseria meningitidis (serogroup B) (154 aa),FASTA scores: opt: 534, E(): 7.6e-29, (50.0% identity in 154 aa overlap); and Q9JSM8|NMA2218 from Neisseria meningitidis (serogroup A) (154 aa), FASTA scores: opt: 526, E(): 2.6e-28, (49.35% identity in 154 aa overlap); Q9HU57|PA5127 from Pseudomonas aeruginosa (153 aa), FASTA scores: opt: 531, E(): 1.2e-28, (52.95% identity in 151 aa overlap); P33899|YIBK_ECOLI|B3606 from Escherichia coli strain K12 (157 aa), FASTA scores: opt: 511, E(): 2.6e-27,(49.35% identity in 154 aa overlap); etc. Belongs to the RNA methyltransferase TrmH family.; Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase) 3778201 spoU 887648 spoU Mycobacterium tuberculosis H37Rv Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase) NP_217883.1 3777737 D 83332 CDS YP_177965.1 57117098 887404 3778568..3780334 1 NC_000962.3 Rv3367, (MTV004.25), len: 588 aa. PE_PGRS51, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O50415|Rv3388|MTV004.46 (731 aa), FASTA scores: opt: 1999, E(): 7.2e-72, (55.0% identity in 620 aa overlap); and MTV004_44, MTV043_65, MTV006_15, MTCY63_2,MTCY21B4_13, MTV023_21, MTV008_43, MTCY24A1_4, MTV023_15; etc. Equivalent to AAK47814 from Mycobacterium tuberculosis strain CDC1551 (628 aa) but shorter 37 aa.; PE-PGRS family protein PE_PGRS51 3780334 PE_PGRS51 887404 PE_PGRS51 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS51 YP_177965.1 3778568 D 83332 CDS NP_217885.1 15610504 887641 complement(3780335..3780979) 1 NC_000962.3 Rv3368c, (MTV004.26c), len: 214 aa. Possible oxidoreductase, equivalent to O07697|MLCL383.23|ML0418 hypothetical 23.6 KDA protein (putative oxidoreductase) from Mycobacterium leprae (210 aa), FASTA scores: opt: 1215, E(): 1.5e-74, (81.4% identity in 210 aa overlap). Also similar to O30106|AF0131 putative NAD(P)H-flavin oxidoreductase from Archaeoglobus fulgidus (194 aa), FASTA scores: opt: 139, E(): 0.028, (29.0% identity in 207 aa overlap); Q60049|NOX_THETH NADH dehydrogenase from Thermus aquaticus (subsp. thermophilus) (205 aa), FASTA scores: opt: 169, E(): 0.00028, (28.3% identity in 212 aa overlap); and shows some similarity to other hypothetical proteins (unknowns or oxidoreductases).; Possible oxidoreductase 3780979 887641 Rv3368c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_217885.1 3780335 R 83332 CDS NP_217886.1 15610505 887669 3780978..3781412 1 NC_000962.3 Rv3369, (MTV004.27), len: 144 aa. Conserved protein. C-terminus is similar to N-terminus of O07696|MLCL383.22c hypothetical 14.7 KDA protein from Mycobacterium leprae (131 aa), FASTA scores: opt: 174, E(): 6e-05, (67.55% identity in 37 aa overlap). Also some slight similarity to Q9EWU1|3SC5B7.08c from Streptomyces coelicolor (153 aa),FASTA scores: opt: 125, E(): 0.13, (31.05% identity in 116 aa overlap).; hypothetical protein 3781412 887669 Rv3369 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217886.1 3780978 D 83332 CDS NP_217887.3 448824810 887259 complement(3781501..3784740) 1 NC_000962.3 Rv3370c, (MTV004.28c), len: 1079 aa. Probable dnaE2,DNA polymerase III, alpha chain (see citations below),similar to many e.g. BAB51086|MLR4428 from Rhizobium loti (Mesorhizobium loti) (1118 aa), FASTA scores: opt: 1103,E(): 8.9e-59, (37.65% identity in 1075 aa overlap); Q9S291|SCI11.28c from Streptomyces coelicolor (1185 aa),FASTA scores: opt: 937, E(): 1e-48, (33.4% identity in 1090 aa overlap); O67125|DP3A_AQUAE|DNAE|AQ_1008 from Aquifex aeolicus (1161 aa), FASTA scores: opt: 895, E(): 3.4e-46,(29.9% identity in 1071 aa overlap); O51526|DP3A_BORBU from Borrelia burgdorferi (Lyme disease spirochete) (1147 aa),FASTA scores: opt: 835, E(): 1.4e-42, (30.05% identity in 888 aa overlap); etc. Equivalent to AAK47817 from Mycobacterium tuberculosis strain CDC1551 (1098 aa) but shorter 19 aa. Also similar to Mycobacterium tuberculosis DP3A_MYCTU|MTCY48.18c|dnaE1 (29.6% identity in 1110 aa overlap). Belongs to DNA polymerase type-C family, DNAE subfamily.; Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase) 3784740 dnaE2 887259 dnaE2 Mycobacterium tuberculosis H37Rv Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase) NP_217887.3 3781501 R 83332 CDS NP_217888.1 15610507 888053 3784932..3786272 1 NC_000962.3 Rv3371, (MTV004.29), len: 446 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to many Mycobacterium tuberculosis (strains H37Rv and CDC1551) hypothetical proteins e.g. O07035|YV30_MYCTU|Rv3130c|MTCY03A2.28|MTCY164.41c (463 aa),FASTA scores: opt: 556, E(): 7.7e-28, (44.95% identity in 447 aa overlap); MTY20B11_9, MTCY28_26, MTV013_8,MTCY21B4_43, MTCY493_29; etc. Also similar to O07692|MLCL383_9|MLCL383.18c hypothetical 14.1 KDA protein from Mycobacterium leprae (129 aa), FASTA scores: opt: 293,E(): 1.3e-11, (47.85% identity in 117 aa overlap).; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 3786272 888053 Rv3371 Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_217888.1 3784932 D 83332 CDS NP_217889.1 15610508 888137 3786314..3787489 1 NC_000962.3 Rv3372, (MTV004.30), len: 391 aa. otsB2,trehalose-6-phosphate phosphatase, equivalent to Q49734|OTSB2|OTSP|B1620_F1_1|MLCL383.17c putative trehalose-phosphatase from Mycobacterium leprae (429 aa),FASTA scores: opt: 1675, E(): 2.4e-91, (67.05% identity in 425 aa overlap). Also weakly similar to several trehalose phosphatases e.g. Q9C8B3|F10O5.8 from Arabidopsis thaliana (Mouse-ear cress) (366 aa), FASTA scores: opt: 432, E(): 3.1e-18, (36.65% identity in 281 aa overlap); O27788|MTH1760 from Methanobacterium thermoautotrophicum (264 aa), FASTA scores: opt: 347, E(): 2.5e-13, (30.75% identity in 221 aa overlap); Q9FWQ2 from Oryza sativa (Rice) (382 aa), FASTA scores: opt: 338, E(): 1.1e-12,(32.5% identity in 320 aa overlap); etc. Also similar to part of Mycobacterium tuberculosis Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), FASTA scores: opt: 1192, E(): 1.6e-62, (56.65% identity in 339 aa overlap).; Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP) 3787489 otsB2 888137 otsB2 Mycobacterium tuberculosis H37Rv Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP) NP_217889.1 3786314 D 83332 CDS NP_217890.1 15610509 888123 3787726..3788367 1 NC_000962.3 Rv3373, (MTV004.31), len: 213 aa. Probable echA18,enoyl-CoA hydratase, similar to others e.g. P97087|CRT from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa), FASTA scores: opt: 423,E(): 3.4e-20, (37.95% identity in 174 aa overlap); Q9X7Q4|SC5F2A.31c from Streptomyces coelicolor (257 aa),FASTA scores: opt: 399, E(): 1.2e-18, (45.05% identity in 171 aa overlap); BAB52005|MLL5584 from Rhizobium loti (Mesorhizobium loti) (257 aa), FASTA scores: opt: 385, E(): 9.6e-18, (41.95% identity in 174 aa overlap); etc. Also some similarity to 3-hydroxybutyryl-CoA dehydratases e.g. P52046|CRT_CLOAB from Clostridium acetobutylicum (261 aa),FASTA scores: opt: 414, E(): 1.3e-19, (38.3% identity in 175 aa overlap). And similar to other hydratases from Mycobacterium tuberculosis e.g. O53418|ECH8_MYCTU|Rv1070c|MT1100|MTV017.23c probable enoyl-CoA hydratase (257 aa), FASTA scores: opt: 365, E(): 1.9e-16, (39.1% identity in 174 aa overlap). Belongs to the enoyl-CoA hydratase/isomerase family. Note that this homology extends across the stop codon and directly into the next ORF MTV004.29, suggesting a possible readthrough of the TGA stop codon.; Probable enoyl-CoA hydratase EchA18 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 3788367 echA18 888123 echA18 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA18 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_217890.1 3787726 D 83332 CDS YP_177966.1 57117099 888100 3788368..3788616 1 NC_000962.3 Rv3374, (MTV004.32), len: 82 aa. Probable echA18.1,enoyl-CoA hydratase C-terminus, similar to the C-terminus of several enoyl-CoA hydratases e.g. Q9I5I4|PA0745 from Pseudomonas aeruginosa (272 aa), FASTA scores: opt: 123,E(): 0.13, (34.55% identity in 81 aa overlap); P97087|CRT from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa),FASTA scores: opt: 115, E(): 0.45, (32.95% identity in 82 aa overlap); Q9I002|PA2841 from Pseudomonas aeruginosa (263 aa), FASTA scores: opt: 108, E(): 1.4, (30.95% identity in 84 aa overlap); etc. Also some similarity to C-terminus of O29956|AF0285 3-hydroxyacyl-CoA dehydrogenase from Archaeoglobus fulgidus (658 aa), FASTA scores: opt: 116,E(): 0.81, (34.15% identity in 82 aa overlap); and other enzymes. And similar to other hydratases from Mycobacterium tuberculosis e.g. O53418|ECH8_MYCTU|Rv1070c|MT1100|MTV017.23c probable enoyl-CoA hydratase (257 aa), FASTA scores: opt: 111, E(): 0.83, (36.05% identity in 86 aa overlap). This homology extends across the upstream TGA stop codon into the upstream ORF MTV004.28, suggesting possible readthrough of the previous stop codon. Note that previously known as echA18'.; Probable enoyl-CoA hydratase (fragment) EchA18.1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 3788616 echA18.1 888100 echA18.1 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase (fragment) EchA18.1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) YP_177966.1 3788368 D 83332 CDS NP_217892.1 15610511 888064 3788621..3790048 1 NC_000962.3 Rv3375, (MTV004.33), len: 475 aa. Probable amiD,amidase, similar to various amidases e.g. Q53116|AMDA enantiomerase-selective amidase from Rhodococcus sp. (462 aa), FASTA scores: opt: 1036, E(): 1.6e-54, (38.6% identity in 464 aa overlap); Q9ZHK8|PZAA nicotinamidase/pyrazinamidase from Mycobacterium smegmatis (468 aa), FASTA scores: opt: 930, E(): 3.4e-48, (36.3% identity in 463 aa overlap); Q9A551|CC2613 pyrazinamidase/nicotinamidase from Caulobacter crescentus (464 aa), FASTA scores: opt: 841, E(): 7.1e-43, (39.45% identity in 469 aa overlap); O69768|AMID_PSEPU amidase from Pseudomonas putida (466 aa), FASTA scores: opt: 800, E(): 2e-40, (33.6% identity in 467 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidu (453 aa), FASTA scores: opt: 669,E(): 1.3e-32, (30.4% identity in 467 aa overlap); etc. Also some similarity to AMIB2|Rv1263|MT1301|MTCY50.19c putative amidase from Mycobacterium tuberculosis (462 aa), (31.5% identity in 466 aa overlap). Seems belong to the amidase family.; Probable amidase AmiD (acylamidase) (acylase) 3790048 amiD 888064 amiD Mycobacterium tuberculosis H37Rv Probable amidase AmiD (acylamidase) (acylase) NP_217892.1 3788621 D 83332 CDS NP_217893.1 15610512 888066 3790156..3790809 1 NC_000962.3 Rv3376, (MTV004.34), len: 217 aa. Hypothetical protein, similar to various bacterial proteins (notably hydrolases) e.g. Q9RUP0|DR1344 hydrolase from Deinococcus radiodurans (222 aa), FASTA scores: opt: 348, E(): 1.8e-15,(36.75% identity in 215 aa overlap); Q9RXA1|DR0414 hydrolase (CBBY/CBBZ/GPH/YIEH family) from Deinococcus radiodurans (155 aa), FASTA scores: opt: 233, E(): 3.5e-08,(36.4% identity in 151 aa overlap); Q9X0Q9|TM1177 conserved hypothetical protein from Thermotoga maritima (225 aa),FASTA scores: opt: 231, E(): 6.6e-08, (27.6% identity in 221 aa overlap); Q9ABI3|CC0244 hydrolase, haloacid dehalogenase-like from Caulobacter crescentus (213 aa),FASTA scores: opt: 213, E(): 9.1e-07, (28.95% identity in 221 aa overlap); BAB38231|ECS4808 putative phosphatase from Escherichia coli strain O157:H7 (206 aa), FASTA scores: opt: 210, E(): 1.4e-06, (26.95% identity in 193 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; hypothetical protein 3790809 888066 Rv3376 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217893.1 3790156 D 83332 CDS NP_217894.1 15610513 888073 complement(3790848..3792353) 1 NC_000962.3 Rv3377c, (MTV004.35c), len: 501 aa. Halimadienyl diphosphate synthase; similarity with various proteins,notably cyclases involved in steroid biosynthesis in plants and bacteria e.g. BAB52679|MLR6369 from Rhizobium loti (Mesorhizobium loti) (516 aa), FASTA scores: opt: 533, E(): 5.6e-27, (30.45% identity in 522 aa overlap); Q9ZTN8 copalyl diphosphate synthase 1 from Cucurbita maxima (Pumpkin) (Winter squash) (823 aa), FASTA scores: opt: 484,E(): 1.2e-23, (28.35% identity in 388 aa overlap); Q38710|AC22 abietadiene cyclase from Abies grandis (868 aa), FASTA scores: opt: 382, E(): 5.2e-17, (25.55% identity in 462 aa overlap); Q41771|AN1 kaurene synthase a from Zea mays (Maize) (823 aa), FASTA scores: opt: 377, E(): 1.1e-16, (29.75% identity in 390 aa overlap); Q9AJE4 diterpene cyclase-1 from Kitasatospora griseola (Streptomyces griseolosporeus) (499 aa), FASTA scores: opt: 336, E(): 3.2e-14, (27.5% identity in 513 aa overlap); Q9SAU6 E-alpha-bisabolene synthase (fragment) from Abies grandis (782 aa), FASTA scores: opt: 317, E(): 7.8e-13,(25.25% identity in 479 aa overlap); etc. Note that this and the upstream ORF MTV004.36c have a significantly lower GC bias than the rest of the genome. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: Mg2+.; Halimadienyl diphosphate synthase 3792353 888073 Rv3377c Mycobacterium tuberculosis H37Rv Halimadienyl diphosphate synthase NP_217894.1 3790848 R 83332 CDS NP_217895.1 15610514 888075 complement(3792358..3793248) 1 NC_000962.3 Rv3378c, (MTV004.36c), len: 296 aa. Diterpene synthase. Note that this ORF and the downstream ORF MTV004.35c have a significantly lower GC bias than the rest of the genome. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: Mg2+.; Diterpene synthase 3793248 888075 Rv3378c Mycobacterium tuberculosis H37Rv Diterpene synthase NP_217895.1 3792358 R 83332 CDS NP_217896.1 15610515 888080 complement(3793257..3794867) 1 NC_000962.3 Rv3379c, (MTV004.37c), len: 536 aa. Probable dxs2,1-deoxy-D-xylulose 5-phosphate synthase, similar to many e.g. Q9F1V2|DXS from Kitasatospora griseola (Streptomyces griseolosporeus) (649 aa), FASTA scores: opt: 1274, E(): 5.4e-71, (50.9% identity in 570 aa overlap); Q9X7W3|DXS_STRCO|SC6A5.17 from Streptomyces coelicolor (656 aa), FASTA scores: opt: 1248, E(): 2.2e-69, (50.55% identity in 568 aa overlap); Q9RBN6|DXS_STRC1 from Streptomyces sp. strain CL190 (631 aa), FASTA scores: opt: 1237, E(): 1e-68, (49.1% identity in 570 aa overlap); Q50000|DXS_MYCLE|TKTB|ML1038 from Mycobacterium leprae (643 aa), FASTA scores: opt: 1215, E(): 2.4e-67, (46.75% identity in 571 aa overlap); Q9R6S7|DXS_SYNLE from Synechococcus leopoliensis (636 aa), FASTA scores: opt: 849, E(): 8.9e-45, (38.55% identity in 550 aa overlap); etc. Also similar to O07184|DXS_MYCTU|Rv2682c|MT2756|MTCY05A6.03c from Mycobacterium tuberculosis (638 aa), FASTA scores: opt: 1226, E(): 4.9e-68, (48.9% identity in 558 aa overlap). Belongs to the transketolase family, DXS subfamily. Cofactor: thiamine pyrophosphate (by similarity). Note that the N-terminus of this putative protein appears to have been interrupted by the adjacent IS6110 element.; Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs2 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) 3794867 dxs2 888080 dxs2 Mycobacterium tuberculosis H37Rv Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs2 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) NP_217896.1 3793257 R 83332 CDS NP_217897.2 448824811 887411 complement(3795100..>3796086) 1 NC_000962.3 Rv3380c, (MTV004.38c), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3380c and Rv3381c, the sequence UUUUAAAG (directly upstream of Rv3380c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa).; Probable transposase >3796086 887411 Rv3380c Mycobacterium tuberculosis H37Rv Probable transposase NP_217897.2 3795100 R 83332 CDS NP_217898.1 15610517 887646 complement(3796035..3796361) 1 NC_000962.3 Rv3381c, (MTV004.39c), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3380c and Rv3381c, the sequence UUUUAAAG (directly upstream of Rv3380c) maybe responsible for such a frameshifting event (see McAdam et al., 1990).; Probable transposase for insertion sequence element IS6110 (fragment) 3796361 887646 Rv3381c Mycobacterium tuberculosis H37Rv Probable transposase for insertion sequence element IS6110 (fragment) NP_217898.1 3796035 R 83332 CDS YP_177967.1 57117100 887953 complement(3796448..3797437) 1 NC_000962.3 Rv3382c, (MTV004.40c), len: 329 aa. Probable lytB1,lytB-related protein, highly similar to many e.g. Q9HVM7|LYTB_PSEAE|PA4557 from Pseudomonas aeruginosa (314 aa), FASTA scores: opt: 1048, E(): 2e-55, (53.2% identity in 314 aa overlap); Q9JR39|LYTB|NMA0624|NMB1831 from Neisseria meningitidis (serogroup a and B) (322 aa), FASTA scores: opt: 1041, E(): 5.4e-55, (52.25% identity in 312 aa overlap); P22565|LYTB_ECOLI|B0029 from Escherichia coli strain K12 (316 aa), FASTA scores: opt: 1013, E(): 2.5e-53,(51.45% identity in 311 aa overlap) (for more information about lytB protein, see citation below); Q9X781|LYTB_MYCLE|LYTB2|ML1938|MLCB1222.06c from Mycobacterium leprae (332 aa), FASTA scores: opt: 979, E(): 2.8e-51, (51.3% identity in 312 aa overlap); etc. Also similar to Q9PAS9|XF2416 drug tolerance protein from Xylella fastidiosa (316 aa), FASTA scores: opt: 1043, E(): 4.1e-55, (53.65% identity in 315 aa overlap). And similar to O53458|Rv1110|LYTB2|MTV017.63 from Mycobacterium tuberculosis (335 aa), FASTA scores: opt: 975, E(): 4.9e-51, (51.3% identity in 312 aa overlap). Belongs to the LytB family.; Probable LYTB-related protein LytB1 3797437 lytB1 887953 lytB1 Mycobacterium tuberculosis H37Rv Probable LYTB-related protein LytB1 YP_177967.1 3796448 R 83332 CDS NP_217900.1 15610519 887680 complement(3797437..3798489) 1 NC_000962.3 Rv3383c, (MTV004.41c), len: 350 aa. Possible idsB,polyprenyl transferase (polyprenyl diphosphate synthase) ,similar to many prenyltransferases involved in lipid biosynthesis e.g. Q9RGW1|GTR geranyl transferase from Streptomyces coelicolor (386 aa), FASTA scores: opt: 908,E(): 3.7e-50, (48.8/% identity in 334 aa overlap); Q9KWG0|GGDPS geranyl geranyl diphosphate synthase from Kitasatospora griseola (Streptomyces griseolosporeus) (348 aa), FASTA scores: opt: 801, E(): 2e-43, (41.5% identity in 347 aa overlap); Q9X7V8|SC6A5.12 putative polyprenyl synthetase from Streptomyces coelicolor (378 aa), FASTA scores: opt: 779, E(): 5.3e-42, (44.45% identity in 324 aa overlap); Q9S5E9 farnesyl, geranylgeranyl, geranylfarnesyl,hexaprenyl, heptaprenyl diphosphate synthase (self-HEPPS) from Synechococcus elongatus (324 aa), FASTA scores: opt: 563, E(): 2.3e-28, (39.85% identity in 241 aa overlap) (see citation below); O26156|IDSA_METTH|MTH50 bifunctional short chain isoprenyl diphosphate synthase [includes: farnesyl pyrophosphate synthetase (FPP synthetase) (dimethylallyltransferase) and geranyltranstransferase] from Methanobacterium thermoautotrophicum (325 aa), FASTA scores: opt: 540, E(): 6.5e-27, (35.75% identity in 319 aa overlap); P95999|GGPP_SULSO|GDS|GDS-1|SSO0061|C05010|C05_049 geranylgeranyl pyrophosphate synthetase (GGPP synthetase) (GGPS) [includes: dimethylallyltransferase and geranyltranstransferase and farnesyltranstransferase] from Sulfolobus solfataricus (332 aa), FASTA scores: opt: 511,E(): 4.5e-25 (36.9% identity in 244 aa overlap); etc. Also similar to Q50727|GGPP_MYCTU|Rv3398c|MT3506|MTCY78.30 probable multifunctional geranylgeranyl pyrophosphate synthetase [includes: dimethylallyltransferase; geranyltranstransferase; farnesyltranstransferase] from Mycobacterium tuberculosis (359 aa), FASTA scores: opt: 687, E(): 3.4e-36, (39.1% identity in 325 aa overlap). Contains PS00723 Polyprenyl synthetases signature 1. Belongs to the FPP/GGPP synthetases family.; Possible polyprenyl synthetase IdsB (polyprenyl transferase) (polyprenyl diphosphate synthase) 3798489 idsB 887680 idsB Mycobacterium tuberculosis H37Rv Possible polyprenyl synthetase IdsB (polyprenyl transferase) (polyprenyl diphosphate synthase) NP_217900.1 3797437 R 83332 CDS NP_217901.1 15610520 887432 complement(3799243..3799635) 1 NC_000962.3 Rv3384c, (MTV004.42c), len: 130 aa. Possible vapC46,toxin, part of toxin-antitoxin (TA) operon with Rv3385c,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. P95252|Rv1962c|MTCY09F9.02 (135 aa), FASTA scores: opt: 266, E(): 1.6e-10, (43.1% identity in 130 aa overlap); and Q50717|YY08_MYCTU|Rv3408|MTCY78.20c (136 aa), FASTA scores: opt: 243, E(): 4.8e-09, (35.1% identity in 131 aa overlap).; Possible toxin VapC46 Contains PIN domain 3799635 vapC46 887432 vapC46 Mycobacterium tuberculosis H37Rv Possible toxin VapC46 Contains PIN domain NP_217901.1 3799243 R 83332 CDS NP_217902.1 15610521 887429 complement(3799635..3799943) 1 NC_000962.3 Rv3385c, (MTV004.43c), len: 102 aa. Possible vapB46,antitoxin, part of toxin-antitoxin (TA) operon with Rv3386c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis hypothetical proteins e.g. Q50718|Y09M_MYCTU|MTCY78.21c|Rv3407|MT3515 (99 aa), FASTA scores: opt: 155, E(): 0.001, (41.05% identity in 78 aa overlap); O07782|Rv0596c|MTCY19H5.26 (85 aa), FASTA scores: opt: 136, E(): 0.016, (39.45% identity in 71 aa overlap); P96916|Rv0626|MTCY20H10.07 (86 aa), FASTA scores: opt: 130,E(): 0.04, (51.2% identity in 41 aa overlap); etc. Also similar to prevent host death (PHD) proteins e.g. CAA66834|PHD from Escherichia coli (73 aa), FASTA scores: opt: 113, E(): 0.45, (39.4% identity in 66 aa overlap); and Q06253|PHD_BPP1 from Bacteriophage P1 (73 aa), FASTA scores: opt: 113, E(): 0.45, (39.4% identity in 66 aa overlap).; Possible antitoxin VapB46 3799943 vapB46 887429 vapB46 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB46 NP_217902.1 3799635 R 83332 CDS NP_217903.1 15610522 888044 3800092..3800796 1 NC_000962.3 Rv3386, (MTV004.44), len: 234 aa. Possible transposase, showing very weak similarity to several is element transposases. Highly similar (but shorter) to P963659|MTCY10G2_13|Rv1036c from Mycobacterium tuberculosis (112 aa), FASTA scores: opt: 507, E(): 8.3e-25, (83.9% identity in 87 aa overlap).; Possible transposase 3800796 888044 Rv3386 Mycobacterium tuberculosis H37Rv Possible transposase NP_217903.1 3800092 D 83332 CDS NP_217904.1 15610523 887820 3800786..3801463 1 NC_000962.3 Rv3387, (MTV004.45), len: 225 aa. Possible transposase, showing very weak similarity to other is element proteins, and similar to various hypothetical proteins.; Possible transposase 3801463 887820 Rv3387 Mycobacterium tuberculosis H37Rv Possible transposase NP_217904.1 3800786 D 83332 CDS YP_177968.1 57117101 888151 3801653..3803848 1 NC_000962.3 Rv3388, (MTV004.46), len: 731 aa. PE_PGRS52, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many PE-family proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O53553|YZ08_MYCTU|RV3508|MTV023.15 (1901 aa), FASTA scores: opt: 2380, E(): 3.6e-87, (53.8% identity in 773 aa overlap); and MTV023_21, MTV023_18,MTV023_14, MTV039_16, MTCY441_4. Predicted to be an outer membrane protein (See Song et al., 2008).; PE-PGRS family protein PE_PGRS52 3803848 PE_PGRS52 888151 PE_PGRS52 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS52 YP_177968.1 3801653 D 83332 CDS NP_217906.1 15610525 887923 complement(3803919..3804791) 1 NC_000962.3 Rv3389c, (MTV004.47c), len: 290 aa. Probable htdY,3-hydroxyacyl-thioester dehydratase (See Gurvitz et al.,2009), shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al., 2005) especially Rv3538. Also shows similarity to members of short-chain dehydrogenases/reductases (SDR) family e.g. Q9L009|SCC30.12c putative dehydrogenase from Streptomyces coelicolor (333 aa), FASTA scores: opt: 602, E(): 2.7e-30,(40.35% identity in 305 aa overlap); Q19058|E04F6.3 hydratase-dehydrogenase-epimerase from Caenorhabditis elegans (298 aa), FASTA scores: opt: 573, E(): 1.6e-28,(41.0% identity in 266 aa overlap); Q9LBK1|PHAJ2|PA1018 (R)-specific enoyl-CoA hydratase from Pseudomonas aeruginosa (288 aa), FASTA scores: opt: 601, E(): 2.7e-30,(40.5% identity in 294 aa overlap).; Probable 3-hydroxyacyl-thioester dehydratase HtdY 3804791 htdY 887923 htdY Mycobacterium tuberculosis H37Rv Probable 3-hydroxyacyl-thioester dehydratase HtdY NP_217906.1 3803919 R 83332 CDS NP_217907.1 15610526 887627 3804865..3805575 1 NC_000962.3 Rv3390, (MTV004.48), len: 236 aa. Probable lpqD, a conserved lipoprotein with some similarity to various bacterial proteins e.g. Q9F3Q7|SC10F4.03 putative isomerase from Streptomyces coelicolor (224 aa), FASTA scores: opt: 416, E(): 2.5e-18, (33.0% identity in 197 aa overlap); Q9ZAX0|PGM 2,3-PDG dependent phosphoglycerate mutase from Amycolatopsis methanolica (205 aa), FASTA scores: opt: 314,E(): 3.7e-12, (28.55% identity in 203 aa overlap); P73454|SLR1748 hypothetical 24.2 KDA protein from Synechocystis sp. strain PCC 6803 (214 aa), FASTA scores: opt: 201, E(): 2.8e-05, (23.8% identity in 189 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical proteins e.g. O53817|Rv0754|MTV041.28 PGRS-family protein (584 aa), FASTA scores: opt: 219, E(): 5.1e-06, (39.8% identity in 226 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LpqD 3805575 lpqD 887627 lpqD Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqD NP_217907.1 3804865 D 83332 CDS NP_217908.1 15610527 887950 3805621..3807573 1 NC_000962.3 Rv3391, (MTV004.49), len: 650 aa. Possible acrA1,multi functional protein with fatty acyl-CoA reductase activity in C-terminal part. Indeed C-terminal part highly similar to P94129|ACR1 fatty acyl-CoA reductase from Acinetobacter calcoaceticus (295 aa), FASTA scores: opt: 767, E(): 1.4e-36, (45.4% identity in 260 aa overlap); and similar to other oxidoreductases dehydrogenases/reductases e.g. Q9Y3A1 CGI-93 protein (similarity with SDR family) from Homo sapiens (Human) (291 aa), FASTA scores: opt: 363,E(): 1.5e-13, (38.65% identity in 194 aa overlap); Q9L146|SC6D11.09 putative oxidoreductase (similarity with SDR family) from Streptomyces coelicolor (343 aa), FASTA scores: opt: 346, E(): 1.6e-12, (30.4% identity in 283 aa overlap); Q9HSR4|YUSZ1|VNG0115G oxidoreductase from Halobacterium sp. strain NRC-1 (260 aa), FASTA scores: opt: 338, E(): 3.7e-12, (33.85% identity in 248 aa overlap); etc. C-terminus also similar to Mycobacterium tuberculosis proteins Q10783|YF43_MYCTU|Rv1543|MTCY48.22c putative oxidoreductase (341 aa), FASTA scores: opt: 787, E(): 1.2e-37, (39.8% identity in 319 aa overlap); O06413|Rv0547c|MTCY25D10.26c hypothetical 31.8 KDA protein (294 aa), FASTA scores: opt: 565, E(): 4.7e-25, (36.8% identity in 242 aa overlap); O53398|Rv1050|MTV017.03 oxidoreductase (SDR family) (301 aa), FASTA scores: opt: 436, E(): 1.1e-17, (32.2% identity in 292 aa overlap). N-terminus (aa 1-320) is similar to P37693|HETM_ANASP polyketide synthase hetM from Anabaena sp. (506 aa), FASTA scores: opt: 188, E(): 1.3e-07, (27.7% identity in 361 aa overlap); so certainly a multi-domain enzyme. Seems to belong to the short-chain dehydrogenases/reductases (SDR) family. Note that this ORF corresponds to the gene ORF2|Q11197 (see Yuan et al., 1995), but longer 266 aa, due to use of a more upstream start site.; Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1 3807573 acrA1 887950 acrA1 Mycobacterium tuberculosis H37Rv Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1 NP_217908.1 3805621 D 83332 CDS NP_217909.1 15610528 887961 complement(3807574..3808437) 1 NC_000962.3 Rv3392c, (MTV004.50), len: 287 aa. CmaA1,cyclopropane mycolic acid synthase 1, characterized in 1995 as CFA1_MYCTU|Q11195|CMAA1|CMA1 cyclopropane-fatty-acyl-phospholipid synthase 1 (see citations below). Highly similar to Mycobacterium tuberculosis proteins MTCY20H10.23c (58.7% identity in 286 aa overlap); MTCY20H10.24c (68.6% identity); MTCY20H10.25c (73.5% identity); MTCY20H10.26c (57.0% identity); and MTCY20G9.30c (55.7% identity). Also highly similar to Q9CBK3|MMAA4|ML1903 methyl mycolic acid synthases from Mycobacterium leprae (298 aa), FASTA scores: opt: 1098,E(): 1e-63, (57.0% identity in 286 aa overlap). Equivalent to AAK44898|MT0672 from Mycobacterium tuberculosis strain CDC1551 (317 aa) but shorter 30 aa and with some differences in residues between the proteins.; Cyclopropane-fatty-acyl-phospholipid synthase 1 CmaA1 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 1) 3808437 cmaA1 887961 cmaA1 Mycobacterium tuberculosis H37Rv Cyclopropane-fatty-acyl-phospholipid synthase 1 CmaA1 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 1) NP_217909.1 3807574 R 83332 CDS NP_217910.1 15610529 887625 3808461..3809387 1 NC_000962.3 Rv3393, (MTV004.51), len: 308 aa. Probable iunH,nucleoside hydrolase, similar to others e.g. Q9RXB2|DR0403 from Deinococcus radiodurans (314 aa), FASTA scores: opt: 497, E(): 6e-24, (34.3% identity in 312 aa overlap); Q27546|IUNH_CRIFA from Crithidia fasciculata (314 aa),FASTA scores: opt: 475, E(): 1.4e-22, (31.45% identity in 318 aa overlap); Q9CK67|IUNH from Pasteurella multocida (310 aa), FASTA scores: opt: 464, E(): 6.9e-22, (30.9% identity in 314 aa overlap); Q9A549|CC2615 from Caulobacter crescentus (323 aa), FASTA scores: opt: 464, E(): 7.2e-22,(37.85% identity in 280 aa overlap); etc. Note that also similar to BAB34113|ECS0690 (alias AAG54985|YBEK) putative tRNA synthetase from Escherichia coli strain O157:H7 (311 aa), FASTA scores: opt: 483, E(): 4.5e-23, (33.0% identity in 315 aa overlap). The active site histidine is conserved.; Probable nucleoside hydrolase IunH (purine nucleosidase) 3809387 iunH 887625 iunH Mycobacterium tuberculosis H37Rv Probable nucleoside hydrolase IunH (purine nucleosidase) NP_217910.1 3808461 D 83332 CDS NP_217911.1 15610530 887945 complement(3809442..3811025) 1 NC_000962.3 Rv3394c, (MTV004.52c), len: 527 aa. Hypothetical protein, with some similarity to various bacterial proteins e.g. BAB51085|MLR4427 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (545 aa), FASTA scores: opt: 267,E(): 2.8e-08, (26.5% identity in 509 aa overlap); BAB48362|MLR0866 DNA damage inducible protein P from Rhizobium loti (Mesorhizobium loti) (438 aa), FASTA scores: opt: 245, E(): 4.6e-07, (25.5% identity in 290 aa overlap); Q9S292|SCI11.27c hypothetical protein from Streptomyces coelicolor (322 aa), FASTA scores: opt: 202, E(): 0.00012,(28.5% identity in 323 aa overlap); etc. Also similarity with P95102|DINP|RV3056|MTCY22D7.25c hypothetical protein from Mycobacterium tuberculosis (346 aa), FASTA scores: opt: 211, E(): 3.9e-05, (26.45% identity in 306 aa overlap). Equivalent to AAK47838 from Mycobacterium tuberculosis strain CDC1551 (492 aa) but longer 35 aa.; hypothetical protein 3811025 887945 Rv3394c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217911.1 3809442 R 83332 CDS NP_217912.2 57117102 887960 complement(3811022..3811636) 1 NC_000962.3 Rv3395c, (MTCY78.33), len: 204 aa. Conserved hypothetical protein, with some similarity with RECA proteins (recombinases A) e.g. P16238|RECA_THIFE from Thiobacillus ferrooxidans (346 aa), FASTA scores: opt: 131,E(): 1.1, (31.45% identity in 140 aa overlap); Q59560|RECA_MYCSM from Mycobacterium smegmatis (349 aa),FASTA scores: opt: 121, E(): 4.4, (30.25% identity in 129 aa overlap); etc. Note that shortened since first submission to avoid overlap with Rv3395A. Equivalent to AAK47839 from Mycobacterium tuberculosis strain CDC1551 (227 aa) but shorter 23 aa.; hypothetical protein 3811636 887960 Rv3395c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217912.2 3811022 R 83332 CDS YP_177969.1 57117103 3205044 3811719..3812345 1 NC_000962.3 Rv3395A, len: 208 aa. Probable membrane protein,with potential transmembrane stretches from aa 7..25 and 55..77. Weak similarity to Q9F2P3|SCE41.16C putative lipoprotein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 107, E(): 7.4, (34.05% identity in 94 aa overlap).; Probable membrane protein 3812345 3205044 Rv3395A Mycobacterium tuberculosis H37Rv Probable membrane protein YP_177969.1 3811719 D 83332 CDS NP_217913.1 15610532 887412 complement(3812501..3814078) 1 NC_000962.3 Rv3396c, (MTCY78.32), len: 525 aa. Probable guaA,gmp synthase (see citation below), equivalent to P46810|GUAA_MYCLE|ML0395|B1620_C2_205 GMP synthase [glutamine-hydrolyzing] from Mycobacterium leprae (529 aa),FASTA scores: opt: 2992, E(): 8.5e-168, (86.85% identity in 525 aa overlap). Also highly similar to others e.g. O52831|GUAA_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (524 aa), FASTA scores: opt: 2636, E(): 5.9e-147, (76.2% identity in 521 aa overlap); Q9L0H2|GUAA_STRCO from Streptomyces coelicolor (526 aa),FASTA scores: opt: 2451, E(): 4.1e-136, (71.55% identity in 513 aa overlap); Q9KF78|GUAA_BACHD from Bacillus Halodurans (513 aa), FASTA scores: opt: 1819, E(): 4.1e-99, (52.55% identity in 510 aa overlap); etc. Contains PS00442 Glutamine amidotransferases class-I active site. Belongs to the type-1 glutamine amidotransferase family in the N-terminal section. And belongs to the GMP synthase family in the C-terminal section.; Probable GMP synthase [glutamine-hydrolyzing] GuaA (glutamine amidotransferase) (GMP synthetase) 3814078 guaA 887412 guaA Mycobacterium tuberculosis H37Rv Probable GMP synthase [glutamine-hydrolyzing] GuaA (glutamine amidotransferase) (GMP synthetase) NP_217913.1 3812501 R 83332 CDS NP_217914.1 15610533 887911 complement(3814090..3814998) 1 NC_000962.3 Rv3397c, (MTCY78.31), len: 302 aa. Probable phyA (alternate gene name: crtB), phytoene synthase, similar to many others e.g. Q9X7V5|SC6A5.09 from Streptomyces coelicolor (312 aa), FASTA scores: opt: 791, E(): 2.8e-43,(48.25% identity in 286 aa overlap); Q9RW07|DR0862 from Deinococcus radiodurans (325 aa), FASTA scores: opt: 482,E(): 1.5e-23, (35.25% identity in 292 aa overlap); Q9JRU9|NMB1168|NMB1130 from Neisseria meningitidis (serogroup B) (290 aa), FASTA scores: opt: 446, E(): 2.8e-21, (34.25% identity in 260 aa overlap); P37272|PSY_CAPAN from Capsicum annuum (Bell pepper) (419 aa), FASTA scores: opt: 431, E(): 3.4e-20, (33.0% identity in 288 aa overlap); etc. Also similar to Q9JUF5|NMA1339 putative poly-isoprenyl transferase from Neisseria meningitidis (serogroup A) (290 aa), FASTA scores: opt: 450, E(): 1.6e-21, (34.6% identity in 260 aa overlap). And similar to crtB|O05424 phytoene synthase from Mycobacterium marinum (319 aa), blastp scores: 113, E= 6e-24, Identities = 89/283 (31%) (see citation below). Contains PS01045 Squalene and phytoene synthases signature 2. Belongs to the phytoene/squalene synthetase family.; Probable phytoene synthase PhyA 3814998 phyA 887911 phyA Mycobacterium tuberculosis H37Rv Probable phytoene synthase PhyA NP_217914.1 3814090 R 83332 CDS YP_177970.1 57117104 887919 complement(3815027..3816106) 1 NC_000962.3 Rv3398c, (MTCY78.30), len: 359 aa. Probable idsA1,geranylgeranyl pyrophosphate synthetase (GGPP synthetase) including: dimethylallyltransferase ,geranyltranstransferase, and farnesyltranstransferase. Most similar to AE000797_3|O26156|Q53479 bifunctional short chain isoprenyl diphosphate synthase from Methanobacterium thermoautotrop (325 aa), FASTA scores: opt: 605, E(): 0,(37.1% identity in 329 aa overlap); homology suggests ATG at 30121 or TTG at 30145 to be the initiation codon. Contains PS00444 Polyprenyl synthetases signature 2. Belongs to the FPP/GGPP synthetases family; belongs to a family that groups together FPP synthetase, GGPP synthetase and hexaprenyl pyrophosphate synthetase. Note that previously known as idsA.; Probable multifunctional geranylgeranyl pyrophosphate synthetase IdsA1 (GGPP synthetase) (ggppsase) (geranylgeranyl diphosphate synthase): dimethylallyltransferase (prenyltransferase) (geranyl-diphosphate synthase) + geranyltranstransferase (farnesyl-diphosphate synthase) (farnesyl-pyrophosphate synthetase) (farnesyl diphosphate synthetase) (FPP synthetase) + farnesyltranstransferase (geranylgeranyl-diphosphate synthase) 3816106 idsA1 887919 idsA1 Mycobacterium tuberculosis H37Rv Probable multifunctional geranylgeranyl pyrophosphate synthetase IdsA1 (GGPP synthetase) (ggppsase) (geranylgeranyl diphosphate synthase): dimethylallyltransferase (prenyltransferase) (geranyl-diphosphate synthase) + geranyltranstransferase (farnesyl-diphosphate synthase) (farnesyl-pyrophosphate synthetase) (farnesyl diphosphate synthetase) (FPP synthetase) + farnesyltranstransferase (geranylgeranyl-diphosphate synthase) YP_177970.1 3815027 R 83332 CDS NP_217916.1 15610535 887938 3816129..3817175 1 NC_000962.3 Rv3399, (MTCY78.29c), len: 348 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to other Mycobacterium tuberculosis (strains H37Rv and CDC1551) hypothetical proteins e.g. P95074|Rv0726c|MTCY210.45c (367 aa), FASTA scores: opt: 1188, E(): 7.7e-69, (60.05% identity in 308 aa overlap); MTCY31.21c (38.0% identity in 308 aa overlap), MTV041_5,MTCY4C12_14, MTY13D12_21, MTV043_22, MTCY210_44, MTCI5_19,MTCI5_20, MTV035_9, MTCY180_22, MTCY31_23, MTY13D12_1,MTCY180_29; etc.; Possible S-adenosylmethionine-dependent methyltransferase 3817175 887938 Rv3399 Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_217916.1 3816129 D 83332 CDS NP_217917.1 15610536 887918 3817239..3818027 1 NC_000962.3 Rv3400, (MTCY78.28c), len: 262 aa. Probable hydrolase, strongly equivalent to Q49741|YY00_MYCLE|ML0393|B1620_F3_119 hypothetical 28.6 KDA protein from Mycobacterium leprae (261 aa), FASTA scores: opt: 1293, E(): 2.2e-71, (74.45% identity in 262 aa overlap). Similar to several various proteins (notably hydrolases) e.g. Q9L2I7|SCF42.32 putative hydrolase from Streptomyces coelicolor (246 aa), FASTA scores: opt: 888,E(): 7.7e-47, (56.35% identity in 245 aa overlap); Q9EX06|2SCG38.13 putative hydrolase from Streptomyces coelicolor (238 aa), FASTA scores: opt: 195, E(): 8.1e-05,(29.5% identity in 234 aa overlap); Q9I5X4|PA0562 probable hydrolase from Pseudomonas aeruginosa (224 aa), FASTA scores: opt: 190, E(): 0.00015, (27.8% identity in 248 aa overlap); O06995|PGMB_BACSU|YVDM putative beta-phosphoglucomutase from Bacillus subtilis (226 aa),FASTA scores: opt: 190, E(): 0.00016, (33.9% identity in 245 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), FASTA scores: opt: 413, E(): 2e-17, (34.9% identity in 238 aa overlap). Interestingly, note that Rv3400 and Rv3401 are similar to beginning and end of Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c with approx. 270 aa missing from the middle.; Probable hydrolase 3818027 887918 Rv3400 Mycobacterium tuberculosis H37Rv Probable hydrolase NP_217917.1 3817239 D 83332 CDS NP_217918.1 15610537 887928 3818042..3820402 1 NC_000962.3 Rv3401, (MTCY78.27c), len: 786 aa. Conserved protein, may be an hydrolase or a transferase, equivalent to Q49736|ML0392|B1620_F1_30 hypothetical 88.1 KDA protein from Mycobacterium leprae (792 aa), FASTA scores: opt: 4820, E(): 0, (91.45% identity in 782 aa overlap). Also highly similar to Q9L2I8|SCF42.31c putative glycosyl transferase from Streptomyces coelicolor (792 aa), FASTA scores: opt: 3060, E(): 2.9e-179, (59.25% identity in 785 aa overlap); and similar to others e.g. Q9K109|NMB0390 maltose phosphorylase from Neisseria meningitidis (serogroup B) (752 aa), FASTA scores: opt: 980, E(): 3.5e-52, (29.2% identity in 774 aa overlap); Q9JSW8|MAPA|NMA2098 putative maltose phosphorylase from Neisseria meningitidis (serogroup A) (752 aa), FASTA scores: opt: 956, E(): 1e-50, (28.4% identity in 764 aa overlap); O06993|YVDK_BACSU hypothetical 88.3 KDA protein (belongs to family 65 of glycosyl hydrolases) from Bacillus subtilis (757 aa), FASTA scores: opt: 926, E(): 6.9e-49,(28.5% identity in 754 aa overlap); Q9CF04|MAPA maltosephosphorylase from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (751 aa), FASTA scores: opt: 907, E(): 1e-47, (26.95% identity in 753 aa overlap); P77154|YCJT_ECOLI|B1316 hypothetical 84.9 KDA protein (belongs to family 65 of glycosyl hydrolases) from Escherichia coli strain K12 (755 aa), FASTA scores: opt: 392, E(): 2.9e-16, (27.5% identity in 774 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), (27.2% identity in 802 aa overlap); note that Rv3400 and Rv3401 are similar to beginning and end of Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c with approx. 270 aa missing from the middle.; hypothetical protein 3820402 887928 Rv3401 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217918.1 3818042 D 83332 CDS NP_217919.1 15610538 887910 complement(3820653..3821891) 1 NC_000962.3 Rv3402c, (MTCY78.26), len: 412 aa. Conserved hypothetical protein, probably involved in cell process,similar to various proteins generally involved in extracellular compounds (lipopolysaccharide O-antigen) biosynthesis e.g. O68392|RFBE perosamine synthetase from Brucella melitensis (367 aa), FASTA scores: opt: 420, E(): 1.2e-19, (26.15% identity in 375 aa overlap); Q9L6C1 3,4-dehydratase-like protein from Streptomyces antibioticus (393 aa), FASTA scores: opt: 419, E(): 1.5e-19, (30.65% identity in 385 aa overlap); Q9RR26|OLENI dehydratase from Streptomyces antibioticus (393 aa), FASTA scores: opt: 416,E(): 2.3e-19, (30.65% identity in 385 aa overlap); O33942 eryciv protein from Saccharopolyspora erythraea (Streptomyces erythraeus) (401 aa), FASTA scores: opt: 410,E(): 5.6e-19, (31.75% identity in 362 aa overlap); Q9UZI4|ASPB-LIKE1|PAB0774 aspartate aminotransferase (ASPB-LIKE1) from Pyrococcus abyssi (366 aa), FASTA scores: opt: 402, E(): 1.7e-18, (27.05% identity in 377 aa overlap); O88001|WLBC putative amino-sugar biosynthesis protein from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (366 aa), FASTA scores: opt: 394, E(): 5.6e-18, (26.8% identity in 347 aa overlap); Q45378|BPLC DNA for lipopolysaccharide biosynthesis from Bordetella pertussis (366 aa), FASTA scores: opt: 393, E(): 6.5e-18,(26.8% identity in 347 aa overlap); etc.; hypothetical protein 3821891 887910 Rv3402c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217919.1 3820653 R 83332 CDS NP_217920.1 15610539 887907 complement(3822262..3823863) 1 NC_000962.3 Rv3403c, (MTCY78.25), len: 533 aa. Hypothetical unknown protein, but some weak similarity to Q9KJP2 hypothetical 54.9 KDA protein from Myxococcus xanthus (504 aa), FASTA scores: opt: 157, E(): 0.011, (24.1% identity in 548 aa overlap).; Hypothetical protein 3823863 887907 Rv3403c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217920.1 3822262 R 83332 CDS NP_217921.1 15610540 887902 complement(3823880..3824584) 1 NC_000962.3 Rv3404c, (MTCY78.24), len: 234 aa. Conserved hypothetical protein, some similarity to several methionyl-tRNA formyltransferases e.g. BAB51418|MLL4854 from Rhizobium loti (Mesorhizobium loti) (317 aa), FASTA scores: opt: 210, E(): 1.7e-06, (27.55% identity in 178 aa overlap); P94463|FMT_BACSU from Bacillus subtilis (317 aa),FASTA scores: opt: 199 ,E(): 8.8e-06, (28.25% identity in 177 aa overlap); O51091||FMT_BORBU|BB0064 from Borrelia burgdorferi (Lyme disease spirochete) (312 aa), FASTA scores: opt: 187, E(): 5.2e-05, (30.2% identity in 192 aa overlap); etc.; hypothetical protein 3824584 887902 Rv3404c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217921.1 3823880 R 83332 CDS NP_217922.1 15610541 887940 complement(3824702..3825268) 1 NC_000962.3 Rv3405c, (MTCY78.23), len: 188 aa. Possible transcriptional regulator, showing weak similarity to other bacterial regulatory proteins e.g. Q9KE70|BH0987 from Bacillus halodurans (203 aa), FASTA scores: opt: 168, E(): 0.0016, (34.8% identity in 92 aa overlap); Q9A5F7|CC2493 Caulobacter crescentus (204 aa), FASTA scores: opt: 160,E(): 0.0051, (32.6% identity in 89 aa overlap); Q9RDR0|SC4A7.02 from Streptomyces coelicolor (227 aa),FASTA scores: opt: 159, E(): 0.0064, (37.0% identity in 189 aa overlap); etc. Also some similarity to hypothetical Mycobacterium tuberculosis regulatory proteins e.g. O05858|Rv3208|MTCY07D11.18c, MTCI125_6, MTCY7D11_18,MTCY10G2_30; etc. Contains potential helix-turn-helix motif from aa 39-60 (+2.97 SD).; Possible transcriptional regulatory protein 3825268 887940 Rv3405c Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_217922.1 3824702 R 83332 CDS NP_217923.1 15610542 887955 3825330..3826217 1 NC_000962.3 Rv3406, (MTCY78.22c), len: 295 aa. Probable dioxygenase, highly similar to Q9WWU|ATSK putative alpha-ketoglutarate dependent dioxygenase from Pseudomonas putida (301 aa), FASTA scores: opt: 994, E(): 3.9e-57,(53.7% identity in 283 aa overlap); Q9I6U1|PA0193 hypothetical protein from Pseudomonas aeruginosa (300 aa),FASTA scores: opt: 1024, E(): 4.4e-59, (53.65% identity in 287 aa overlap); Q9HX81|TAUD|PA3935 taurine dioxygenase from Pseudomonas aeruginosa (277 aa), FASTA scores: opt: 599, E(): 1.4e-31, (39.35% identity in 277 aa overlap); and similar to other dioxygenases e.g. AAG54718|TAUD (alias BAB33845|ECS0422) taurine dioxygenase 2-oxoglutarate-dependent from Escherichia coli strain O157:H7 (283 aa), FASTA scores: opt: 595, E(): 2.5e-31,(38.1% identity in 281 aa overlap); etc. Belongs to the TfdA family of dioxygenases.; Probable dioxygenase 3826217 887955 Rv3406 Mycobacterium tuberculosis H37Rv Probable dioxygenase NP_217923.1 3825330 D 83332 CDS NP_217924.1 15610543 887505 3826252..3826551 1 NC_000962.3 Rv3407, (MTCY78.21c), len: 99 aa. Possible vapB47,antitoxin, part of toxin-antitoxin (TA) operon with Rv3408,see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK46285|MT2013 (90 aa), FASTA scores: opt: 160, E(): 0.00021, (37.1% identity in 89 aa overlap); O50412|Rv3385c|MTV004.43c (102 aa), FASTA scores: opt: 155, E(): 0.00051, (41.05% identity in 78 aa overlap), MTCY19H5.26, MTCY20H10.07, MTI376.09c,MTCY427.21, etc.; Possible antitoxin VapB47 3826551 vapB47 887505 vapB47 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB47 NP_217924.1 3826252 D 83332 CDS NP_217925.1 15610544 887900 3826548..3826958 1 NC_000962.3 Rv3408, (MTCY78.20c), len: 136 aa. Possible vapC47,toxin, part of toxin-antitoxin (TA) operon with Rv3407,contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O50411|Rv3384c|MTV004.42c (130 aa), FASTA scores: opt: 243, E(): 1.7e-09, (35.1% identity in 131 aa overlap); P95252|Rv1962c|MTCY09F9.02 (135 aa), FASTA scores: opt: 191, E(): 5e-06, (35.5% identity in 138 aa overlap), etc.; Possible toxin VapC47 Contains PIN domain 3826958 vapC47 887900 vapC47 Mycobacterium tuberculosis H37Rv Possible toxin VapC47 Contains PIN domain NP_217925.1 3826548 D 83332 CDS NP_217926.1 15610545 887502 complement(3826991..3828727) 1 NC_000962.3 Rv3409c, (MTCY78.19), len: 578 aa. ChoD, cholesterol oxidase, equivalent to Q9CCV1|CHOD|ML0389 (alias Q59530|CHOD|B1620_C3_240) putative cholesterol oxidase from Mycobacterium leprae (569 aa), FASTA scores: opt: 3510,E(): 3.8e-198, (88.6% identity in 569 aa overlap). Belongs to the GMC oxidoreductases family. Cofactor: FAD flavoprotein. Contains PS00017 ATP/GTP-binding site motif A.; Cholesterol oxidase ChoD (cholesterol-O2 oxidoreductase) 3828727 choD 887502 choD Mycobacterium tuberculosis H37Rv Cholesterol oxidase ChoD (cholesterol-O2 oxidoreductase) NP_217926.1 3826991 R 83332 CDS NP_217927.1 15610546 887510 complement(3828783..3829910) 1 NC_000962.3 Rv3410c, (MTCY78.18), len: 375 aa. Probable guaB3,inosine-5'-monophosphate (imp) dehydrogenase, equivalent to Q49721|YY10_MYCLE|ML0388|B1620_C2_193 hypothetical 38.9 KDA protein from Mycobacterium leprae (375 aa), FASTA scores: opt: 2182, E(): 9.5e-122, (90.6% identity in 373 aa overlap). Highly similar to Q9RHY9 GUAB ORF genes for imp dehydrogenase, hypothetical protein from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (376 aa), FASTA scores: opt: 1490, E(): 7.6e-81, (61.0% identity in 382 aa overlap); Q9L0I6|SCD63.03 putative inosine-5'-monophosphate dehydrogenase from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1275, E(): 3.8e-68, (52.95% identity in 372 aa overlap); P73853|GUAB|SLR1722 imp dehydrogenase subunit from Synechocystis sp. strain PCC 6803 (387 aa), FASTA scores: opt: 882, E(): 6.7e-45, (41.3% identity in 373 aa overlap); and similar to other inosine-5'-monophosphate dehydrogenases e.g. P44334|IMDH_HAEIN|GUAB|HI0221 from Haemophilus influenzae (488 aa), FASTA scores: opt: 267,E(): 1.8e-08, (34.25% identity in 216 aa overlap); etc. Also highly similar to the C-terminus of Q50753|GUAA/B homology to Mycobacterium leprae GUAA (fragment) from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 506, E(): 4.6e-23, (85.05% identity in 87 aa overlap). Similar to other eukaryotic and prokaryotic IMPDH and to GMP reductase.; Probable inosine-5'-monophosphate dehydrogenase GuaB3 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase) (imp oxidoreductase) (inosine-5'-monophosphate oxidoreductase) (IMPDH) (IMPD) 3829910 guaB3 887510 guaB3 Mycobacterium tuberculosis H37Rv Probable inosine-5'-monophosphate dehydrogenase GuaB3 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase) (imp oxidoreductase) (inosine-5'-monophosphate oxidoreductase) (IMPDH) (IMPD) NP_217927.1 3828783 R 83332 CDS NP_217928.1 15610547 887498 complement(3829930..3831519) 1 NC_000962.3 Rv3411c, (MTCY78.17), len: 529 aa. Probable guaB2,inosine-5'-monophosphate (imp) dehydrogenase, equivalent to Q49729|IMDH_MYCLE|GUAB|ML0387|B1620_C3_238 inosine-5'-monophosphate dehydrogenase from Mycobacterium leprae (529 aa), FASTA scores: opt: 3154, E(): 4.4e-165,(92.45% identity in 529 aa overlap). Highly similar to other inosine-5'-monophosphate dehydrogenases e.g. Q9RHZ0|GUAB from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (506 aa), FASTA scores: opt: 2284, E(): 1.5e-117, (67.9% identity in 505 aa overlap); Q9L0I7|SCD63.02 from Streptomyces coelicolor (501 aa),FASTA scores: opt: 2178, E(): 9e-112, (67.2% identity in 491 aa overlap); O67820|IMDH_AQUAE|GUAB|AQ_2023 from Aquifex aeolicus (490 aa), FASTA scores: opt: 1820, E(): 3.2e-92, (58.1% identity in 487 aa overlap); etc. Also similar to Q50716|YY10_MYCTU|Rv3410c|MT3518|MTCY78.18 hypothetical 38.9 KDA protein from Mycobacterium tuberculosis (38.6% identity in 158 aa overlap). Contains PS00487 imp dehydrogenase / GMP reductase signature. Similar to other eukaryotic and prokaryotic IMPDH and to GMP reductase.; Probable inosine-5'-monophosphate dehydrogenase GuaB2 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase) (imp oxidoreductase) (inosine-5'-monophosphate oxidoreductase) (IMPDH) (IMPD) 3831519 guaB2 887498 guaB2 Mycobacterium tuberculosis H37Rv Probable inosine-5'-monophosphate dehydrogenase GuaB2 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase) (imp oxidoreductase) (inosine-5'-monophosphate oxidoreductase) (IMPDH) (IMPD) NP_217928.1 3829930 R 83332 CDS NP_217929.1 15610548 887499 3831726..3832136 1 NC_000962.3 Rv3412, (MTCY78.16c), len: 136 aa. Hypothetical protein, strongly similar to Q49742|YY12_MYCLE|ML0386|B1620_F3_131 hypothetical 15.3 KDA protein from Mycobacterium leprae (137 aa), FASTA scores: opt: 933, E(): 6.3e-52, (93.4% identity in 136 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3832136 887499 Rv3412 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217929.1 3831726 D 83332 CDS NP_217930.1 15610549 887925 complement(3832146..3833045) 1 NC_000962.3 Rv3413c, (MTCY78.16), len: 299 aa. Unknown ala-,pro-rich protein.; Unknown alanine and proline rich protein 3833045 887925 Rv3413c Mycobacterium tuberculosis H37Rv Unknown alanine and proline rich protein NP_217930.1 3832146 R 83332 CDS NP_217931.1 15610550 887594 complement(3833038..3833676) 1 NC_000962.3 Rv3414c, (MTCY78.15), len: 212 aa. Probable sigD,alternative RNA polymerase sigma-D factor (see citations below), similar to others (notably from Streptomyces coelicolor) e.g. Q9L0I8|SCD63.01 from Streptomyces coelicolor (195 aa), FASTA scores: opt: 533, E(): 9.6e-28,(47.25% identity in 182 aa overlap); Q9FDS3|ADSA from Streptomyces griseus (258 aa), FASTA scores: opt: 223, E(): 1.8e-07, (28.95% identity in 183 aa overlap); BAB48649|MLL1224 from Rhizobium loti (Mesorhizobium loti) (187 aa), FASTA scores: opt: 202, E(): 3.2e-06, (30.4% identity in 194 aa overlap); P38133|RPOE_STRCO|SIGE|SCE94.07 from Streptomyces coelicolor (176 aa), FASTA scores: opt: 200, E(): 4.1e-06,(35.25% identity in 156 aa overlap); P37978|CNRH_ALCEU from Alcaligenes eutrophus (Ralstonia eutropha), FASTA scores: opt: 197, E(): 6.9e-06, (30.35% identity in 191 aa overlap); etc. C-terminus strongly similar to N-terminal part of Q49727|S1620B|B1620_C3_233 hypothetical 6.2 KDA protein from Mycobacterium leprae (59 aa), FASTA scores: opt: 217, E(): 1.3e-07, (90.25% identity in 41 aa overlap). Belongs to the sigma-70 factor family.; Probable alternative RNA polymerase sigma-D factor SigD 3833676 sigD 887594 sigD Mycobacterium tuberculosis H37Rv Probable alternative RNA polymerase sigma-D factor SigD NP_217931.1 3833038 R 83332 CDS NP_217932.1 15610551 887599 complement(3833694..3834521) 1 NC_000962.3 Rv3415c, (MTCY78.14), len: 275 aa. Conserved hypothetical protein, equivalent to Q9CCV3|ML0383 hypothetical protein from Mycobacterium leprae (281 aa),FASTA scores: opt: 1278, E(): 4.2e-71, (73.5% identity in 279 aa overlap). Also some similarity with P71677|RIBD_MYCTU|RIBG|Rv1409|MT1453|MTCY21B4.26 riboflavin biosynthesis protein R (339 aa), FASTA scores: opt: 143,E(): 0.13, (28.25% identity in 184 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 3834521 887599 Rv3415c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217932.1 3833694 R 83332 CDS NP_217933.1 15610552 887598 3834892..3835200 1 NC_000962.3 Rv3416, (MTCY78.13c), len: 102 aa. WhiB3 (alternate gene name: whmB), WhiB-like regulatory protein (see citations below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q49871|WHIB3|WHIB|ML0382|B229_F1_2|B1620_F3_137 probable transcription factor WHIB3 from Mycobacterium leprae (102 aa), FASTA scores: opt: 657, E(): 7.9e-39, (86.25% identity in 102 aa overlap). Also highly similar to Q9Z6E9|WHIB3 from Mycobacterium smegmatis (96 aa), FASTA scores: opt: 604, E(): 3.5e-35, (80.4% identity in 102 aa overlap); and O88103|WHID|SC6G4.45c|WBLB from Streptomyces coelicolor (112 aa), FASTA scores: opt: 437, E(): 1.4e-23, (62.5% identity in 96 aa overlap). Also similar to O05847|WHIB1|Rv3219|MTCY07D11.07c from Mycobacterium tuberculosis (84 aa), FASTA scores: opt: 215, E(): 2.5e-08,(44.45% identity in 81 aa overlap). Note that primer extension analysis revealed three transcriptional start sites and that expression from the three potential promoters is growth phase-dependent (see Mulder et al.,1999). Moreover, the transcription of this CDS seems to be activated in macrophages (see Ramakrishnan et al., 2000). [4Fe-4S] cluster is degraded by oxygen and reacts with no (See Singh et al., 2007).; Transcriptional regulatory protein WhiB-like WhiB3 Contains [4FE-4S] cluster 3835200 whiB3 887598 whiB3 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein WhiB-like WhiB3 Contains [4FE-4S] cluster NP_217933.1 3834892 D 83332 CDS NP_217934.1 15610553 887877 complement(3835272..3836891) 1 NC_000962.3 Rv3417c, (MTCY78.12), len: 539 aa. GroEL1 (alternate genbe name: cpn60_1), 60 kDa chaperonin 1 (protein cpn60 1) (see citations below), equivalent to P37578|CH61_MYCLE|B1620_C3_228|GROL1|GROEL1|GROEL- 1|GROE1|ML0381|B229_ 60 KDA chaperonin 1 from Mycobacterium leprae (537 aa),FASTA scores: opt: 2846, E(): 1.5e-154, (82.95% identity in 539 aa overlap). Also highly similar to others e.g. Q00767|CH61_STRAL|GROL1|GROEL1 from Streptomyces albus G (539 aa), FASTA scores: opt: 2130, E(): 8.1e-114, (61.9% identity in 541 aa overlap); P40171|CH61_STRCO|GROL1|GROEL1|SC6G4.40 from Streptomyces coelicolor (540 aa), FASTA scores: opt: 2119, E(): 3.4e-113, (61.8% identity in 542 aa overlap); etc. Also similar to P06806|CH62_MYCTU|Q48931|Rv0440|MTV037.04|GROL2|GROEL2|GRO EL-2|HSP65 (62.2% identity in 527 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00296 Chaperonins cpn60 signature. Belongs to the chaperonin (HSP60) family.; 60 kDa chaperonin 1 GroEL1 (protein CPN60-1) (GroEL protein 1) 3836891 groEL1 887877 groEL1 Mycobacterium tuberculosis H37Rv 60 kDa chaperonin 1 GroEL1 (protein CPN60-1) (GroEL protein 1) NP_217934.1 3835272 R 83332 CDS NP_217935.1 15610554 887583 complement(3836986..3837288) 1 NC_000962.3 Rv3418c, (MTCY78.11), len: 100 aa. GroES (alternate gene names: cpn10, mpt57), 10 kDa chaperonin (protein cpn10) (see citations below), equivalent to P24301|CH10_MYCLE|MOPB|GROES|CHPA|ML0380|B1620_C3_227|B229 _C3_247 from Mycobacterium leprae (99 aa), FASTA scores: opt: 568,E(): 2.1e-31, (89.9% identity in 99 aa overlap). And also strongly identical to others e.g. O86017|CH10_MYCAV|MOPB|GROES from Mycobacterium avium and Mycobacterium paratuberculosis (99 aa), FASTA scores: opt: 611, E(): 2.9e-34, (96.95% identity in 99 aa overlap); P15020|CH10_MYCBO|MOPB|GROES from Mycobacterium bovis (99 aa), FASTA scores: opt: 596, E(): 2.9e-33, (98.95% identity in 94 aa overlap); P40172|CH10_STRCO|GROES|SC6G4.39 from Streptomyces coelicolor and Streptomyces lividans (102 aa),FASTA scores: opt: 480, E(): 1.6e-25, (76.75% identity in 99 aa overlap); etc. Also identical to MSG10KAG_1,MT10KAG_1, MTBCGA_1. Contains PS00681 Chaperonins cpn10 signature. Belongs to the GROES chaperonin family.; 10 kDa chaperonin GroES (protein CPN10) (protein GroES) (BCG-a heat shock protein) (10 kDa antigen) 3837288 groES 887583 groES Mycobacterium tuberculosis H37Rv 10 kDa chaperonin GroES (protein CPN10) (protein GroES) (BCG-a heat shock protein) (10 kDa antigen) NP_217935.1 3836986 R 83332 CDS NP_217936.1 15610555 887595 complement(3837555..3838589) 1 NC_000962.3 Rv3419c, (MTCY78.10), len: 344 aa. Probable gcp,glycoprotease, equivalent to P37969|GCP_MYCLE|GCP|ML0379|U229E|U1620c|B229_C3_246|B1620 _C3_226 probable glycoprotease from Mycobacterium leprae (351 aa),FASTA scores: opt: 1898, E(): 2.4e-101, (86.1% identity in 345 aa overlap). Highly similar to others e.g. O86793|GCP_STRCO|GCP|SC6G4.30 from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1282, E(): 4.1e-66, (60.45% identity in 344 aa overlap); Q9WXZ2|TM0145 from Thermotoga maritima (327 aa), FASTA scores: opt: 867, E(): 1.9e-42,(45.4% identity in 337 aa overlap); P05852|GCP_ECOLI|B3064 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 838, E(): 9e-41, (46.55% identity in 346 aa overlap); etc. Shows some similarity to Q50707|YY21_MYCTU|Rv3421c|MTCY78.08 (33.9% identity in 127 aa overlap). Contains PS01016 Glycoprotease family signature. Belongs to peptidase family M22; also known as the glycoprotease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable O-sialoglycoprotein endopeptidase Gcp (glycoprotease) 3838589 gcp 887595 gcp Mycobacterium tuberculosis H37Rv Probable O-sialoglycoprotein endopeptidase Gcp (glycoprotease) NP_217936.1 3837555 R 83332 CDS NP_217937.1 15610556 887555 complement(3838586..3839062) 1 NC_000962.3 Rv3420c, (MTCY78.09), len: 158 aa. Probable rimI,ribosomal-protein-alanine acetyltransferase, contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to C-terminal part of Q49857|YY21_MYCLE|ML0378|B229_C1_170 hypothetical 38.0 KDA protein from Mycobacterium leprae (359 aa), FASTA scores: opt: 772, E(): 2.7e-44, (72.1% identity in 154 aa overlap). Similar notably to ribosomal-protein-alanine acetyltransferases e.g. Q9AC11|CC0058 from Caulobacter crescentus (150 aa), FASTA scores: opt: 223, E(): 4.9e-08,(37.5% identity in 136 aa overlap); Q9KFD4|BH0547 from Bacillus halodurans (151 aa), FASTA scores: opt: 222, E(): 5.8e-08, (35.2% identity in 142 aa overlap); Q9PG61|XF0441 from Xylella fastidiosa (156 aa), FASTA scores: opt: 207,E(): 5.9e-07, (32.2% identity in 149 aa overlap); Q9HVB7|RIMI|PA4678 from Pseudomonas aeruginosa (150 aa),FASTA scores: opt: 203, E(): 1.1e-06, (32.45% identity in 151 aa overlap); P09453|RIMI_ECOLI|B4373 from Escherichia coli strain K12 (148 aa), FASTA scores: opt: 196, E(): 3.1e-06, (33.55% identity in 149 aa overlap); etc. Belongs to the acetyltransferase family, RIMI subfamily.; Ribosomal-protein-alanine acetyltransferase RimI (acetylating enzyme for N-terminal of ribosomal protein S18) 3839062 rimI 887555 rimI Mycobacterium tuberculosis H37Rv Ribosomal-protein-alanine acetyltransferase RimI (acetylating enzyme for N-terminal of ribosomal protein S18) NP_217937.1 3838586 R 83332 CDS NP_217938.1 15610557 887532 complement(3839059..3839694) 1 NC_000962.3 Rv3421c, (MTCY78.08), len: 211 aa. Conserved hypothetical protein, equivalent to Q49857|YY21_MYCLE|ML0378|B229_C1_170 hypothetical 38.0 KDA protein from Mycobacterium leprae (359 aa), FASTA scores: opt: 1000, E(): 1.8e-50, (75.6% identity in 205 aa overlap). Also similar to other hypothetical bacterial proteins e.g. O86791|SC6G4.28 from Streptomyces coelicolor (217 aa), FASTA scores: opt: 453, E(): 3.3e-19, (48.1% identity in 212 aa overlap); Q9AC10|CC0059 (glycoprotease family protein) from Caulobacter crescentus (211 aa), FASTA scores: opt: 248, E(): 2e-07, (34.3% identity in 210 aa overlap); Q9KQK9|VC1989 from Vibrio cholerae (237 aa),FASTA scores: opt: 238, E(): 8.2e-07, (28.85% identity in 208 aa overlap); BAB51966|Mlr5530 from Rhizobium loti (Mesorhizobium loti) (225 aa), FASTA scores: opt: 237, E(): 9e-07, (35.0% identity in 220 aa overlap); etc. Some similarity to upstream Q50709|GCP_MYCTU|Rv3419c|MT3528|MTCY78.10 from Mycobacterium tuberculosis (344 aa), (33.9% identity in 127 aa overlap).; hypothetical protein 3839694 887532 Rv3421c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217938.1 3839059 R 83332 CDS NP_217939.1 15610558 887557 complement(3839691..3840197) 1 NC_000962.3 Rv3422c, (MTCY78.07), len: 168 aa. Conserved hypothetical protein, equivalent to Q49864|YY22_MYCLE|ML0377|U229F|B229_C2_205 hypothetical 17.6 KDA protein from Mycobacterium leprae (161 aa), FASTA scores: opt: 752, E(): 8.3e-38, (77.4% identity in 146 aa overlap). Also similar to other hypothetical bacterial proteins e.g. O86788|YJEE_STRCO|SC6G4.25 from Streptomyces coelicolor (148 aa), FASTA scores: opt: 377, E(): 1.2e-15,(50.85% identity in 120 aa overlap); Q9X1W7|TM1632 from Thermotoga maritima (161 aa), FASTA scores: opt: 247, E(): 6.2e-08, (39.4% identity in 137 aa overlap); Q9RRY1|DR2351 from Deinococcus radiodurans (148 aa), FASTA scores: opt: 236, E(): 2.6e-07, (38.6% identity in 127 aa overlap); etc. Contains PS00017 ATP /GTP-binding site motif A.; hypothetical protein 3840197 887557 Rv3422c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217939.1 3839691 R 83332 CDS NP_217940.1 15610559 887634 complement(3840194..3841420) 1 NC_000962.3 Rv3423c, (MTCY78.06), len: 408 aa. Alr, alanine racemase, equivalent to P38056|ALR_MYCLE|ML0375|B229_C3_243 alanine racemase from Mycobacterium leprae (388 aa), FASTA scores: opt: 2160, E(): 2.3e-124, (84.35% identity in 384 aa overlap). Also highly similar to other alanine racemases e.g. Q9L888|ALR_MYCAV from Mycobacterium avium (391 aa),FASTA scores: opt: 2103, E(): 6.8e-121, (83.6% identity in 384 aa overlap); P94967|ALR_MYCSM from Mycobacterium smegmatis (389 aa), FASTA scores: opt: 1721, E(): 1.3e-97,(67.25% identity in 385 aa overlap); O86786|ALR_STRCO|SC6G4.23 from Streptomyces coelicolor (391 aa), FASTA scores: opt: 1041, E(): 3.7e-56, (47.65% identity in 380 aa overlap); etc. Contains Pfam entry PF00842 Alanine racemase. Belongs to the alanine racemase family.; Alanine racemase Alr 3841420 alr 887634 alr Mycobacterium tuberculosis H37Rv Alanine racemase Alr NP_217940.1 3840194 R 83332 CDS NP_217941.1 15610560 887618 complement(3841714..3842076) 1 NC_000962.3 Rv3424c, (MTCY78.05), len: 120 aa. Hypothetical unknown protein.; Hypothetical protein 3842076 887618 Rv3424c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217941.1 3841714 R 83332 CDS YP_177971.1 57117105 887635 3842239..3842769 1 NC_000962.3 Rv3425, (MTCY78.04c), len: 176 aa. PPE57, Member of the M. tuberculosis PPE family, similar to many e.g. O06246|Rv3429|MTCY77.01 (178 aa), FASTA scores: opt: 781,E(): 7e-44, (69.9% identity in 176 aa overlap); and downstream Q50702|YY26_MYCTU|Rv3426|MTCY78.03c (232 aa),FASTA scores: opt: 517, E(): 1.2e-26, (68.0% identity in 125 aa overlap); MTV049_11, MTCY428_16, MTV049_22,MTV049_30, MTCY261_4; etc.; PPE family protein PPE57 3842769 PPE57 887635 PPE57 Mycobacterium tuberculosis H37Rv PPE family protein PPE57 YP_177971.1 3842239 D 83332 CDS YP_177972.1 57117106 887622 3843036..3843734 1 NC_000962.3 Rv3426, (MTCY78.03c), len: 232 aa. PPE58, Member of the M. tuberculosis PPE family, similar to many e.g. the downstream O06246|Rv3429|MTCY77.01 (178 aa), FASTA scores: opt: 555, E(): 6.5e-26, (72.0% identity in 125 aa overlap); and upstream Q50703|YY25_MYCTU|Rv3425|MTCY78.04c (176 aa),FASTA scores: opt: 517, E(): 1.1e-23, (68.0% identity in 125 aa overlap); MTV049_30, MTCY3C7_24, MTCY428_16,MTCY3A2_22; etc.; PPE family protein PPE58 3843734 PPE58 887622 PPE58 Mycobacterium tuberculosis H37Rv PPE family protein PPE58 YP_177972.1 3843036 D 83332 CDS NP_217944.1 15610563 887631 complement(3843885..3844640) 1 NC_000962.3 Rv3427c, (MTCY78.02), len: 251 aa. Possible transposase, similar to other e.g. Q9APG8|ORF2 putative transposase subunit 2 from Pseudomonas putida (251 aa),FASTA scores: opt: 479, E(): 1.8e-21, (34.85% identity in 238 aa overlap). Contains PS00017 ATP/GTP-binding site motif A.; Possible transposase 3844640 887631 Rv3427c Mycobacterium tuberculosis H37Rv Possible transposase NP_217944.1 3843885 R 83332 CDS NP_217945.1 15610564 887621 complement(3844738..3845970) 1 NC_000962.3 Rv3428c, (MTCY78.01, len: 410 aa. Possible transposase insertion sequence, similar to others e.g. Q9APG9|ORF1 from Pseudomonas putida (509 aa), FASTA scores: opt: 578, E(): 1.1e-29, (32.45% identity in 376 aa overlap); P55379|Y4BL_RHISN from Rhizobium sp. strain NGR234 (516 aa), FASTA scores: opt: 665, E(): 2.7e-35,(35.3% identity in 391 aa overlap); etc.; Possible transposase 3845970 887621 Rv3428c Mycobacterium tuberculosis H37Rv Possible transposase NP_217945.1 3844738 R 83332 CDS YP_177973.1 57117107 887630 3847165..3847701 1 NC_000962.3 Rv3429, (MTCY77.01), len: 178 aa. PPE59, Member of the M. tuberculosis PPE family, similar to many e.g. the upstream Q50703|YY25_MYCTU|Rv3425|MTCY78.04c (176 aa),FASTA scores: opt: 781, E(): 1.9e-44, (69.9% identity in 176 aa overlap); and Q50702|YY26_MYCTU|Rv3426|MTCY78.03c (232 aa), FASTA scores: opt: 555, E(): 1.7e-29, (72.0% identity in 125 aa overlap) (but diverges at 3' end); etc.; PPE family protein PPE59 3847701 PPE59 887630 PPE59 Mycobacterium tuberculosis H37Rv PPE family protein PPE59 YP_177973.1 3847165 D 83332 CDS NP_217947.1 15610566 887615 complement(3847642..3848805) 1 NC_000962.3 Rv3430c, (MTCY77.02c), len: 387 aa. Possible IS1540 transposase, similar to several e.g. Q49592 transposase from Mycobacterium intracellulare (340 aa), FASTA scores: opt: 1377, E(): 1.6e-81, (64.2% identity in 338 aa overlap); similarity is lost at C-terminus due to possible frameshift after aa 297.; Possible transposase 3848805 887615 Rv3430c Mycobacterium tuberculosis H37Rv Possible transposase NP_217947.1 3847642 R 83332 CDS NP_217948.1 15610567 887608 complement(3849294..3850139) 1 NC_000962.3 Rv3431c, (MTCY77.03c), len: 281 aa. Possible truncated transposase for IS1552, similar to, but shorter than other transposases e.g. P72303 from Rhodococcus opacus (418 aa), FASTA scores: opt: 1509, E(): 1.2e-91, (80.95% identity in 278 aa overlap); Q9AKV5 from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 1115, E(): 7.8e-66, (63.45% identity in 268 aa overlap); etc.; Possible transposase (fragment) 3850139 887608 Rv3431c Mycobacterium tuberculosis H37Rv Possible transposase (fragment) NP_217948.1 3849294 R 83332 CDS NP_217949.1 15610568 887580 complement(3850372..3851754) 1 NC_000962.3 Rv3432c, (MTCY77.04c), len: 460 aa. Probable gadB,glutamate decarboxylase, similar to many e.g. P73043|gad|SLL1641 from Synechocystis sp. strain PCC 6803 (467 aa), FASTA scores: opt: 1684, E(): 6.2e-99, (55.35% identity in 457 aa overlap); Q9X8J5|SCE9.23 from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1650,E(): 8.9e-97, (57.4% identity in 446 aa overlap); Q9AQU4|gad from Oryza sativa (Rice) (501 aa), FASTA scores: opt: 1498, E(): 3.7e-87, (51.6% identity in 432 aa overlap); Q07346|DCE_PETHY from Petunia hybrida (Petunia) (500 aa), FASTA scores: opt: 1485, E(): 2.5e-86, (51.15% identity in 437 aa overlap); etc. Belongs to group II decarboxylases (DDC, gad, HDC and TYRDC).; Probable glutamate decarboxylase GadB 3851754 gadB 887580 gadB Mycobacterium tuberculosis H37Rv Probable glutamate decarboxylase GadB NP_217949.1 3850372 R 83332 CDS NP_217950.1 15610569 887571 complement(3851792..3853213) 1 NC_000962.3 Rv3433c, (MTCY77.05), len: 473 aa. Conserved protein, member of YKL151c/yjeF family, equivalent to P37391|YY33_MYCLE|ML0373|U229G|B229_C2_201 hypothetical 47.2 KDA protein from Mycobacterium leprae (473 aa), FASTA scores: opt: 2650, E(): 5e-136, (84.55% identity in 473 aa overlap). Also similar to other hypothetical bacterial proteins e.g. Q9X3W3 from Zymomonas mobilis (484 aa), FASTA scores: opt: 700, E(): 1.2e-30, (33.7% identity in 484 aa overlap); O86783|SC6G4.20c from Streptomyces coelicolor (485 aa), FASTA scores: opt: 563, E(): 3.2e-23, (48.45% identity in 489 aa overlap); Q9LC81 from Arthrobacter sp. Q36 (313 aa), FASTA scores: opt: 553, E(): 7.9e-23, (44.2% identity in 303 aa overlap); etc. Contains Pfam match to entry PF01256 hypothetical UPFOO31 family signature and PF03853 YjeF-related protein N-terminus. Belongs to the UPF0031 family.; hypothetical protein 3853213 887571 Rv3433c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217950.1 3851792 R 83332 CDS NP_217951.1 15610570 887573 complement(3853215..3853928) 1 NC_000962.3 Rv3434c, (MTCY77.06c), len: 237 aa. Possible conserved transmembrane protein, showing some similarity with Q9CGH7|YLDB hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (258 aa),FASTA scores: opt: 248, E(): 1.6e-09, (28.8% identity in 198 aa overlap); and P94983|Rv1648|MTCY06H11.13 from Mycobacterium tuberculosis (268 aa), FASTA scores: opt: 205, E(): 1.2e-06, (31.45% identity in 194 aa overlap).; Possible conserved transmembrane protein 3853928 887573 Rv3434c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_217951.1 3853215 R 83332 CDS NP_217952.1 15610571 887564 complement(3853939..3854793) 1 NC_000962.3 Rv3435c, (MTCY77.07c), len: 284 aa. Probable conserved transmembrane protein, showing some similarity with P95061|Rv0713|MTCY210.32 hypothetical 33.9 KDA protein from Mycobacterium tuberculosis (313 aa), FASTA scores: opt: 557, E(): 1.3e-26, (35.8% identity in 282 aa overlap); and O32991|MLCB2492.12 from Mycobacterium leprae (95 aa),FASTA scores: opt: 150, E(): 0.022, (35.3% identity in 85 aa overlap). Equivalent to AAK47881 from Mycobacterium tuberculosis strain CDC1551 (312 aa) but shorter 28 aa.; Probable conserved transmembrane protein 3854793 887564 Rv3435c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_217952.1 3853939 R 83332 CDS NP_217953.1 15610572 887568 complement(3855015..3856889) 1 NC_000962.3 Rv3436c, (MTCY77.08c), len: 624 aa. Probable glmS,glucosamine--fructose-6-phosphate aminotransferase,equivalent to P40831|GLMS_MYCLE|ML0371|B229_C3_238 glucosamine--fructose-6-phosphate aminotransferase [isomerizing] from Mycobacterium leprae (623 aa), FASTA scores: opt: 3584, E(): 4.7e-214, (89.3% identity in 627 aa overlap). Also highly similar to others e.g. O68956|GLMS_MYCSM from Mycobacterium smegmatis (627 aa),FASTA scores: opt: 3517, E(): 6.5e-210, (87.25% identity in 627 aa overlap); O86781|GLMS_STRCO|SC6G4.18 from Streptomyces coelicolor (614 aa), FASTA scores: opt: 2364,E(): 1.3e-138, (64.95% identity in 625 aa overlap); Q9K1P9|NMB0031 from Neisseria meningitidis (serogroup B) and Q9JWN9|GLMS|NMA0276 from Neisseria meningitidis (serogroup A) (612 aa), FASTA scores: opt: 1445, E(): 8.4e-82, (43.55% identity in 627 aa overlap); etc. Belongs to the type-2 gatase domain in the N-terminal section. Belongs to the sis family, GLMS subfamily, in the C-terminal section.; Probable glucosamine--fructose-6-phosphate aminotransferase [isomerizing] GlmS (hexosephosphate aminotransferase) (D-fructose-6-phosphate amidotransferase) (GFAT) (L-glutamine-D-fructose-6-phosphate amidotransferase) (glucosamine-6-phosphate synthase) 3856889 glmS 887568 glmS Mycobacterium tuberculosis H37Rv Probable glucosamine--fructose-6-phosphate aminotransferase [isomerizing] GlmS (hexosephosphate aminotransferase) (D-fructose-6-phosphate amidotransferase) (GFAT) (L-glutamine-D-fructose-6-phosphate amidotransferase) (glucosamine-6-phosphate synthase) NP_217953.1 3855015 R 83332 CDS NP_217954.1 15610573 887567 3856911..3857387 1 NC_000962.3 Rv3437, (MTCY77.09), len: 158 aa. Questionable ORF. Possible conserved transmenbrane protein, C-terminus similar to N-terminal part of O06345|Rv3482c|MTCY13E12.35c hypothetical 28.5 KDA protein from Mycobacterium tuberculosis (260 aa), FASTA scores: opt: 140, E(): 0.1,(58.8% identity in 34 aa overlap); and Q9XAN5|SC4C6.05c putative membrane protein from Streptomyces (347 aa),coelicolor FASTA scores: opt: 112, E(): 6.8, (50.0% identity in 32 aa overlap).; Possible conserved transmembrane protein 3857387 887567 Rv3437 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_217954.1 3856911 D 83332 CDS NP_217955.1 15610574 887561 3857397..3858239 1 NC_000962.3 Rv3438, (MTCY77.10), len: 280 aa. Conserved protein,equivalent to Q9CCV6|ML0370 hypothetical protein from Mycobacterium leprae (289 aa), FASTA scores: opt: 1491,E(): 9.2e-81, (79.85% identity in 283 aa overlap); and highly similar (but shorter 41 aa) to Q49872|B229_F1_20 hypothetical 34.0 KDA protein from Mycobacterium leprae (324 aa), FASTA scores: opt: 1491, E(): 1e-80, (79.85% identity in 283 aa overlap). Shows some similarity to Q9KIU3|LIPA lipase from plasmid pAH114 uncultured bacterium (281 aa), FASTA scores: opt: 168, E(): 0.0081, (29.3% identity in 140 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; hypothetical protein 3858239 887561 Rv3438 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217955.1 3857397 D 83332 CDS NP_217956.1 15610575 887565 complement(3858259..3859662) 1 NC_000962.3 Rv3439c, (MTCY77.11c), len: 467 aa. Conserved hypothetical ala-, pro-rich protein, similar in part to N-terminal part of Q49853|B229_C1_154 hypothetical 11.2 KDA protein from Mycobacterium leprae (103 aa), FASTA scores: opt: 265, E(): 0.0013, (51.1% identity in 90 aa overlap).; Conserved hypothetical alanine and proline rich protein 3859662 887565 Rv3439c Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine and proline rich protein NP_217956.1 3858259 R 83332 CDS NP_217957.1 15610576 887592 complement(3859665..3859976) 1 NC_000962.3 Rv3440c, (MTCY77.12c), len: 103 aa. Hypothetical unknown protein.; Hypothetical protein 3859976 887592 Rv3440c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_217957.1 3859665 R 83332 CDS NP_217958.1 15610577 887589 complement(3860024..3861370) 1 NC_000962.3 Rv3441c, (MTCY77.13c), len: 448 aa. Probable mrsA,phosphoglucomutase or phosphomannomutase, equivalent to Q49869|URED|B229_C3_234 MRSA protein homolog from Mycobacterium leprae (463 aa), FASTA scores: opt: 2449,E(): 6.3e-135, (87.65% identity in 445 aa overlap); and highly similar (but longer 178 aa) to Q49862|UREC|B229_C2_192 putative urease operon UREC protein from Mycobacterium leprae (288 aa), FASTA scores: opt: 1442, E(): 1.3e-76, (86.5% identity in 267 aa overlap). Highly similar to phospho-sugar mutases e.g. Q53876|SC6G4.14 putative phospho-sugar mutase (similar to phosphomannomutases) from Streptomyces coelicolor (452 aa),FASTA scores: opt: 1710, E(): 5e-92, (60.45% identity in 450 aa overlap); Q9KG46|BH0267 phosphoglucosamine mutase from Bacillus halodurans (447 aa), FASTA scores: opt: 1351,E(): 3.5e-71, (48.4% identity in 444 aa overlap); BAB58323|GLMM phosphoglucosamine-mutase from Staphylococcus aureus subsp. aureus Mu50 (451 aa) and Q99QR5|GLMM(FEMD)|SA1965 phosphoglucosamine-mutase from Staphylococcus aureus subsp. aureus N315. (451 aa), FASTA scores: opt: 1315, E(): 4.3e-69, (48.45% identity in 446 aa overlap); P95685|FEMD|GLMM phosphoglucosamine-mutase (451 aa), FASTA scores: opt: 1310, E(): 8.5e-69, (48.2% identity in 446 aa overlap); P95575|MRSA_PSESY MRSA protein homolog from Pseudomonas syringae (pv. syringae) (447 aa), FASTA scores: opt: 1143, E(): 4.2e-59, (42.75% identity in 447 aa overlap); etc. Contains PS00710 Phosphoglucomutase and phosphomannomutase phosphoserine signature. Belongs to the phosphohexose mutases family.; Probable phospho-sugar mutase / MrsA protein homolog 3861370 mrsA 887589 mrsA Mycobacterium tuberculosis H37Rv Probable phospho-sugar mutase / MrsA protein homolog NP_217958.1 3860024 R 83332 CDS NP_217959.1 15610578 887488 complement(3861495..3861950) 1 NC_000962.3 Rv3442c, (MTCY77.14c), len: 151 aa. rpsI, 30S ribosomal protein S9, equivalent to P40828|RS9_MYCLE|ML0365|B229_C2_191 30S ribosomal protein S9 (153 aa), FASTA scores: opt: 800, E(): 2.1e-42, (83.85% identity in 155 aa overlap). Also highly similar to others e.g. Q53875|RS9_STRCO|SC6G4.13 from Streptomyces coelicolor (170 aa), FASTA scores: opt: 533, E(): 5.7e-26, (60.75% identity in 135 aa overlap); Q9KGD4|RPSI|BH0169 (BS10) from Bacillus halodurans (130 aa), FASTA scores: opt: 469, E(): 3.8e-22, (58.65% identity in 121 aa overlap); Q9CDG7|RPSI from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (130 aa), FASTA scores: opt: 451, E(): 4.9e-21,(58.65% identity in 121 aa overlap); P07842|RS9_BACST|RPSI from Bacillus stearothermophilus (129 aa), FASTA scores: opt: 448, E(): 7.4e-21, (54.55% identity in 121 aa overlap); etc. Contains PS00360 Ribosomal protein S9 signature. Belongs to the S9P family of ribosomal proteins.; 30S ribosomal protein S9 RpsI 3861950 rpsI 887488 rpsI Mycobacterium tuberculosis H37Rv 30S ribosomal protein S9 RpsI NP_217959.1 3861495 R 83332 CDS NP_217960.1 15610579 887584 complement(3861947..3862390) 1 NC_000962.3 Rv3443c, (MTCY77.15c), len: 147 aa. rplM, 50S ribosomal protein L13, equivalent to P38014|RL13_MYCLE|RPLM|ML0364|B229_C3_232 from Mycobacterium leprae (147 aa), FASTA scores: opt: 917, E(): 7.5e-53, (91.15% identity in 147 aa overlap). Also highly similar to others e.g. Q53874|RL13_STRCO|RPLM|SC6G4.12 from Streptomyces coelicolor (147 aa), FASTA scores: opt: 668,E(): 1.1e-36, (65.5% identity in 145 aa overlap); Q9X1G5|RL13_THEMA|RPLM|TM1454 from Thermotoga maritima (149 aa), FASTA scores: opt: 536, E(): 4.4e-28, (53.65% identity in 136 aa overlap); O67722|RL13_AQUAE|RPLM|AQ_1877 from Aquifex aeolicus (144 aa), FASTA scores: opt: 529, E(): 1.2e-27, (53.2% identity in 141 aa overlap); etc. Belongs to the L13P family of ribosomal proteins.; 50S ribosomal protein L13 RplM 3862390 rplM 887584 rplM Mycobacterium tuberculosis H37Rv 50S ribosomal protein L13 RplM NP_217960.1 3861947 R 83332 CDS NP_217961.1 15610580 887587 complement(3862624..3862926) 1 NC_000962.3 Rv3444c, (MTCY77.16c), len: 100 aa. EsxT, ESAT-6 like protein (see citation below), equivalent to Q9CCV7|ML0363 possible secreted protein from Mycobacterium leprae (104 aa), FASTA scores: opt: 362, E(): 1.1e-18,(71.25% identity in 73 aa overlap). C-terminal part highly similar to Q49852|B229_C1_150 hypothetical 5.3 KDA protein from Mycobacterium leprae (49 aa), FASTA scores: opt: 227,E(): 1.4e-09, (68.9% identity in 45 aa overlap). Seems to belong to the ESAT6 family.; Putative ESAT-6 like protein EsxT 3862926 esxT 887587 esxT Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxT NP_217961.1 3862624 R 83332 CDS NP_217962.2 448824812 887585 complement(3862947..3863264) 1 NC_000962.3 Rv3445c, (MTCY77.17c), len: 105 aa. EsxU, ESAT-6 like protein (see citations below), showing weak similarity to O30373|VCD|PA2257 pyoverdine biosynthesis protein from Pseudomonas aeruginosa (215 aa), FASTA scores: opt: 103,E(): 5.6, (32.35% identity in 133 aa overlap). Seems to belong to the ESAT6 family. Start changed since first submission (-20 aa).; ESAT-6 like protein EsxU 3863264 esxU 887585 esxU Mycobacterium tuberculosis H37Rv ESAT-6 like protein EsxU NP_217962.2 3862947 R 83332 CDS NP_217963.1 15610582 887586 complement(3863317..3864531) 1 NC_000962.3 Rv3446c, (MTCY77.18c), len: 404 aa. Hypothetical unknown ala-, val-rich protein.; Hypothetical alanine and valine rich protein 3864531 887586 Rv3446c Mycobacterium tuberculosis H37Rv Hypothetical alanine and valine rich protein NP_217963.1 3863317 R 83332 CDS NP_217964.1 15610583 887581 complement(3864528..3868238) 1 NC_000962.3 Rv3447c, (MTCY77.19c), len: 1236 aa. EccC4, esx conserved component, ESX-4 type VII secretion system protein, probable membrane protein, similar to various bacterial proteins e.g. O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 1186, E(): 1.9e-60, (42.9% identity in 1312 aa overlap); Q9L0T6|SCD35.15c from Streptomyces coelicolor (1525 aa), FASTA scores: opt: 932, E(): 9.2e-46, (27.2% identity in 1374 aa overlap); Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa), FASTA scores: opt: 910, E(): 1.5e-44, (34.4% identity in 1319 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 805, E(): 1.9e-38,(25.85% identity in 1292 aa overlap); etc. The C-terminal region is similar to Q9CDD7|ML0052 (alias O33086|MLCB628.15c) hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 850, E(): 2.3e-41,(35.2% identity in 588 aa overlap); and O6973|Rv3871|MTV027.06 hypothetical protein from Mycobacterium tuberculosis (591 aa), FASTA scores: opt: 845, E(): 4.3e-41, (35.3% identity in 586 aa overlap). N-terminal part shows similarity with hypothetical proteins from Mycobacterium tuberculosis e.g. O69735|Rv3870|MTV027.05 (747 aa), FASTA scores: opt: 761,E(): 3.6e-36, (38.2% identity in 746 aa overlap). Equivalent to AAK47893 from Mycobacterium tuberculosis strain CDC1551 (1200 aa) but longer 36 aa. Contains three of PS00017 ATP/GTP-binding site motif A (P-loop).; ESX conserved component EccC4 ESX-4 type VII secretion system protein Probable membrane protein 3868238 eccC4 887581 eccC4 Mycobacterium tuberculosis H37Rv ESX conserved component EccC4 ESX-4 type VII secretion system protein Probable membrane protein NP_217964.1 3864528 R 83332 CDS NP_217965.1 15610584 887624 3868352..3869755 1 NC_000962.3 Rv3448, (MTCY77.20), len: 467 aa. EccD4, esx conserved component, ESX-4 type VII secretion system protein, probable integral membrane protein, showing some similarity with Q9CD35|ML2529 from Mycobacterium leprae (485 aa), FASTA scores: opt: 371, E(): 3.6e-14, (27.25% identity in 481 aa overlap); and two proteins from Mycobacterium tuberculosis O86362|Rv0290|MTV035.18 (472 aa), FASTA scores: opt: 429, E(): 1.6e-17, (28.6% identity in 479 aa overlap); and O05457|Rv3887c|MTCY15F10.25 (509 aa), FASTA scores: opt: 203, E(): 0.00019, (25.6% identity in 492 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature.; ESX conserved component EccD4 ESX-4 type VII secretion system protein Probable integral membrane protein 3869755 eccD4 887624 eccD4 Mycobacterium tuberculosis H37Rv ESX conserved component EccD4 ESX-4 type VII secretion system protein Probable integral membrane protein NP_217965.1 3868352 D 83332 CDS NP_217966.1 15610585 887602 3869752..3871119 1 NC_000962.3 Rv3449, (MTCY13E12.02), len: 455 aa. Probable mycP4,membrane-anchored serine protease (mycosin) (see citation below), similar to hypothetical unknowns or proteases from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK48366|MT3998 subtilase family protein from Mycobacterium tuberculosis strain CDC1551 (411 aa), FASTA scores: opt: 747, E(): 3.5e-33, (45.65% identity in 416 aa overlap); O05461|Rv3883c|MTCY15F10.29 membrane-anchored mycosin MYCP1 (446 aa), FASTA scores: opt: 747, E(): 3.8e-33, (45.45% identity in 451 aa overlap); O53695|Rv0291|MTV035.19 probable membrane-anchored mycosin MYCP2 (461 aa), FASTA scores: opt: 660, E(): 1.9e-28, (44.0% identity in 457 aa overlap); etc. And similar to hypothetical proteases from Mycobacterium leprae e.g. O33076|MLCB628.04|ML0041 hypothetical 45.7 KDA protein (probable secreted protease) (446 aa), FASTA scores: opt: 683, E(): 1.1e-29, (43.8% identity in 450 aa overlap); Q9CD36|ML2528 putative protease (475 aa), FASTA scores: opt: 608, E(): 1.3e-25,(43.0% identity in 451 aa overlap); Q9CBV3|ML1538 possible protease (567 aa), FASTA scores: opt: 389, E(): 9.7e-14,(33.8% identity in 562 aa overlap); etc. Also some similarity to other proteases from several organisms e.g. O31788|APRX alkaline serine protease from Bacillus subtilis (442 aa), FASTA scores: opt: 296, E(): 8.3e-09, (29.4% identity in 313 aa overlap); O86650|SC3C3.17c putative secreted serine protease from Streptomyces coelicolor (450 aa), FASTA scores: opt: 279, E(): 7e-08, (33.55% identity in 343 aa overlap); Q9KBJ7|APRX|BH193 intracellular alkaline serine protease from Bacillus halodurans (444 aa),FASTA scores: opt: 257, E(): 1.1e-06, (28.65% identity in 335 aa overlap); O86642|SC3C3.08 serine protease from Streptomyces coelicolor (413 aa), FASTA scores: opt: 243,E(): 5.7e-06, (38.25% identity in 387 aa overlap); etc. Has putative signal peptide at N-terminus and hydrophobic stretch at C-terminus. Contains three signatures typical of subtilase family: aspartic acid active site (PS00136),histidine active site (PS00137), and serine active site (PS00138). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily.; Probable membrane-anchored mycosin MycP4 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-4) 3871119 mycP4 887602 mycP4 Mycobacterium tuberculosis H37Rv Probable membrane-anchored mycosin MycP4 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-4) NP_217966.1 3869752 D 83332 CDS NP_217967.1 15610586 887619 complement(3871084..3872496) 1 NC_000962.3 Rv3450c, (MTCY13E12.03c), len: 470 aa. EccB4, esx conserved component, ESX-4 type VII secretion system protein, probable membrane protein (possible membrane spanning region near N-terminus). Similar to hypothetical unknowns proteins from Mycobacterium leprae e.g. O33088|MLCB628.17C|ML0054 hypothetical 51.9 KDA protein (putative membrane protein)(481 aa), FASTA scores: opt: 708, E(): 6.4e-32, (32.9% identity in 480 aa overlap); Q9CD29|ML2536 (552 aa), FASTA scores: opt: 394, E(): 1.7e-14, (33.6% identity in 503 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O69734|Rv3869|MTV027.04 (480 aa), FASTA scores: opt: 717, E(): 2e-32, (32.55% identity in 479 aa overlap); O05449|Rv3895c|MTCY15F10.17 (495 aa), FASTA scores: opt: 670, E(): 8.3e-30, (36.4% identity in 475 aa overlap); O5368|Rv0283|MTV035.11 (538 aa), FASTA scores: opt: 467, E(): 1.5e-18, (36.3% identity in 493 aa overlap); etc.; ESX conserved component EccB4 ESX-4 type VII secretion system protein Probable membrane protein 3872496 eccB4 887619 eccB4 Mycobacterium tuberculosis H37Rv ESX conserved component EccB4 ESX-4 type VII secretion system protein Probable membrane protein NP_217967.1 3871084 R 83332 CDS NP_217968.2 57117108 887611 3872617..3873405 1 NC_000962.3 Rv3451, (MTCY13E12.04), len: 262 aa. Probable cut3,cutinase precursor, similar to others e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 540, E(): 3.5e-24, (43.4% identity in 219 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 214, E(): 2e-05,(31.45% identity in 210 aa overlap); Q9Y7G8 from Pyrenopeziza brassicae (203 aa), FASTA scores: opt: 203,E(): 8.5e-05, (31.05% identity in 190 aa overlap); P29292|CUTI_ASCRA from Ascochyta rabiei (223 aa), FASTA scores: opt: 155, E(): 0.054, (31.65% identity in 120 aa overlap). Similar to other proteins from Mycobacterium tuberculosis e.g. the downstream ORF O06319|Rv3452|MTCY13E12.05 hypothetical 23.1 KDA protein (226 aa), FASTA scores: opt: 775, E(): 1e-37, (58.65% identity in 220 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 565, E(): 1.3e-25, (44.85% identity in 223 aa overlap); Q10837|CUT1_MYCTU|Rv1984c|MT2037|MTCY39.35 probable cutinase precursor (217 aa), FASTA scores: opt: 489, E(): 3e-21, (47.05% identity in 221 aa overlap); etc. Equivalent to AAK47897 from Mycobacterium tuberculosis strain CDC1551 (247 aa) but longer 15 aa. Contains cutinase, serine active site motif (PS00155). Belongs to the cutinase family. Alternative start possible at 3733. Start changed since first submission (+15 aa).; Probable cutinase precursor Cut3 3873405 cut3 887611 cut3 Mycobacterium tuberculosis H37Rv Probable cutinase precursor Cut3 NP_217968.2 3872617 D 83332 CDS NP_217969.1 15610588 887610 3873452..3874132 1 NC_000962.3 Rv3452, (MTCY13E12.05), len: 226 aa. Probable cut4,cutinase precursor, similar to other e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 522, E(): 7.3e-24, (46.6% identity in 221 aa overlap); P30272|CUTI_MAGGR|CUT1 from Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) (228 aa), FASTA scores: opt: 205, E(): 3.8e-05, (29.25% identity in 164 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 204, E(): 3.9e-05,(33.5% identity in 209 aa overlap); etc. Similar to other proteins from Mycobacterium tuberculosis e.g. upstream ORF O06318|CUT3_MYCTU|Rv3451|MT3557|MTCY13E12.04 probable cutinase precursor (247 aa), FASTA scores: opt: 773, E(): 1.3e-38, (59.35% identity in 209 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 704, E(): 1.3e-34, (53.4% identity in 219 aa overlap); etc. Contains PS00155 Cutinase, serine active site. Belongs to the cutinase family. Alternative start possible at 4553 in cSCY13E12 but no RBS.; Probable cutinase precursor Cut4 3874132 cut4 887610 cut4 Mycobacterium tuberculosis H37Rv Probable cutinase precursor Cut4 NP_217969.1 3873452 D 83332 CDS NP_217970.1 15610589 887596 3874404..3874736 1 NC_000962.3 Rv3453, (MTCY13E12.06), len: 110 aa. Possible conserved transmembrane protein, showing weak similarity with other proteins e.g. Q9F6C3 putative ABC transporter from Propionibacterium thoenii (424 aa), FASTA scores: opt: 104, E(): 6.8, (40.6% identity in 69 aa overlap).; Possible conserved transmembrane protein 3874736 887596 Rv3453 Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_217970.1 3874404 D 83332 CDS NP_217971.1 15610590 887613 3874822..3876090 1 NC_000962.3 Rv3454, (MTCY13E12.07), len: 422 aa. Probable conserved integral membrane protein, showing some similarity to various proteins (generally transporters) e.g. Q9I5C8|PA0811 probable MFS transporter from Pseudomonas aeruginosa (415 aa), FASTA scores: opt: 145,E(): 0.13, (28.2% identity in 188 aa overlap); Q01266|YHYC_PSESN hypothetical protein in HYUC 3'region (ORF 5) (fragment) from Pseudomonas sp. strain NS671 (245 aa), FASTA scores: opt: 130, E(): 0.75, (24.65% identity in 134 aa overlap); Q9I242|PA2073 probable transporter (membrane subunit) from Pseudomonas aeruginosa (476 aa),FASTA scores: opt: 125, E(): 2.5, (24.6% identity in 252 aa overlap); etc. Equivalent to AAK47900 from Mycobacterium tuberculosis strain CDC1551 (562 aa) but shorter 140 aa. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved integral membrane protein 3876090 887613 Rv3454 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_217971.1 3874822 D 83332 CDS NP_217972.3 448824813 887590 complement(3876052..3876822) 1 NC_000962.3 Rv3455c, (MTCY13E12.08c), len: 256 aa. Probable truA, pseudouridine synthase A, equivalent to Q9X796|TRUA_MYCLE|ML1955|MLCB1222.25c tRNA pseudouridine synthase a from Mycobacterium leprae (249 aa), FASTA scores: opt: 1345, E(): 3.2e-80, (77.25% identity in 246 aa overlap). Also highly similar to others e.g. O86776|TRUA_STRCO|SC6G4.09 from Streptomyces coelicolor (284 aa), FASTA scores: opt: 595, E(): 1.7e-31, (49.8% identity in 259 aa overlap); Q9RS37|DR2290 from Deinococcus radiodurans (280 aa), FASTA scores: opt: 383, E(): 1e-17,(41.2% identity in 216 aa overlap); Q9PJT0|TRUA_CHLMU|TC0748 from Chlamydia muridarum (267 aa),FASTA scores: opt: 334, E(): 1.5e-14, (37.65% identity in 231 aa overlap); P07649|TRUA_ECOLI|hist|ASUC|LEUK|B2318 from Escherichia coli strain K12 (270 aa), FASTA scores: opt: 315, E(): 2.5e-13, (33.35% identity in 240 aa overlap); etc. Belongs to the TruA family of pseudouridine synthases.; Probable tRNA pseudouridine synthase a TruA (pseudouridylate synthase I) (pseudouridine synthase I) (uracil hydrolyase) 3876822 truA 887590 truA Mycobacterium tuberculosis H37Rv Probable tRNA pseudouridine synthase a TruA (pseudouridylate synthase I) (pseudouridine synthase I) (uracil hydrolyase) NP_217972.3 3876052 R 83332 CDS NP_217973.1 15610592 887523 complement(3876890..3877432) 1 NC_000962.3 Rv3456c, (MTCY13E12.09c), len: 180 aa. rplQ, 50S ribosomal protein L17, equivalent to Q9X797|RL17_MYCLE|ML1956|MLCB1222.26c 50S ribosomal protein L17 from Mycobacterium leprae (170 aa), FASTA scores: opt: 874, E(): 9.5e-45, (81.85% identity in 171 aa overlap). Also highly similar to other e.g. O86775|RL17_STRCO|SC6G4.08 from Streptomyces coelicolor (168 aa), FASTA scores: opt: 609, E(): 3.7e-29, (60.0% identity in 170 aa overlap); BAB47931|MLR0326 from Rhizobium loti (Mesorhizobium loti) (143 aa), FASTA scores: opt: 404, E(): 3.7e-17, (49.65% identity in 139 aa overlap); Q9Z9H5|RL17_THETH|RPLQ from Thermus aquaticus (subsp. thermophilus) (118 aa), FASTA scores: opt: 366,E(): 5.5e-15, (53.15% identity in 111 aa overlap); P02416|RL17_ECOLI|RPLQ|B3294 from Escherichia coli strain K12 (127 aa), FASTA scores: opt: 347, E(): 7.6e-14, (50.4% identity in 119 aa overlap); etc. Belongs to the L17P family of ribosomal proteins.; 50S ribosomal protein L17 RplQ 3877432 rplQ 887523 rplQ Mycobacterium tuberculosis H37Rv 50S ribosomal protein L17 RplQ NP_217973.1 3876890 R 83332 CDS NP_217974.1 15610593 887629 complement(3877464..3878507) 1 NC_000962.3 Rv3457c, (MTCY13E12.10c), len: 347 aa. Probable rpoA, alpha chain of RNA polymerase, equivalent to Q9X798|RPOA_MYCLE|ML1957|MLCB1222.27c DNA-directed RNA polymerase alpha from Mycobacterium leprae (347 aa), FASTA scores: opt: 2139, E(): 1.3e-123, (95.65% identity in 347 aa overlap). Also highly similar to others e.g. P72404|RPOA_STRCO|C6G4.07 from Streptomyces coelicolor (340 aa), FASTA scores: opt: 1672, E(): 4.7e-95, (75.55% identity in 348 aa overlap); Q9X4V6|RPOA_STRGT from Streptomyces granaticolor (340 aa), FASTA scores: opt: 1671, E(): 5.4e-95, (75.55% identity in 348 aa overlap); P20429|RPOA_BACSU from Bacillus subtilis (314 aa), FASTA scores: opt: 939, E(): 3e-50, (48.9% identity in 311 aa overlap); etc. Contains (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the RNA polymerase alpha chain family.; Probable DNA-directed RNA polymerase (alpha chain) RpoA (transcriptase alpha chain) (RNA polymerase alpha subunit) (DNA-directed RNA nucleotidyltransferase) 3878507 rpoA 887629 rpoA Mycobacterium tuberculosis H37Rv Probable DNA-directed RNA polymerase (alpha chain) RpoA (transcriptase alpha chain) (RNA polymerase alpha subunit) (DNA-directed RNA nucleotidyltransferase) NP_217974.1 3877464 R 83332 CDS NP_217975.1 15610594 887620 complement(3878659..3879264) 1 NC_000962.3 Rv3458c, (MTCY13E12.11c), len: 201 aa. rpsD, 30S ribosomal protein S4, equivalent to Q9X799|RS4_MYCLE|RPSD|ML1958|MLCB1222.28c 30S ribosomal protein S4 from Mycobacterium leprae (201 aa), FASTA scores: opt: 1271, E(): 2.2e-73, (93.5% identity in 201 aa overlap); and P45811|RS4_MYCBO|RPSD from Mycobacterium bovis (131 aa), FASTA scores: opt: 867, E(): 4.9e-48,(100.0% identity in 130 aa overlap). Also highly similar to others e.g. P81288|RS4_BACST|RPSD from Bacillus stearothermophilus (198 aa), FASTA scores: opt: 665, E(): 4e-35, (52.25% identity in 201 aa overlap); Q9K7Z8|RPSD|BH3209 from Bacillus halodurans (200 aa), FASTA scores: opt: 626, E(): 1.2e-32, (48.75% identity in 203 aa overlap); Q9X1I3|RS4_THEMA|RPSD|TM1473 from Thermotoga maritima (209 aa), FASTA scores: opt: 591, E(): 2e-30,(45.0% identity in 209 aa overlap); etc. Contains ribosomal protein S4 signature (PS00632) and ATP/GTP binding site motif A (PS00017). Belongs to the S4P family of ribosomal proteins.; 30S ribosomal protein S4 RpsD 3879264 rpsD 887620 rpsD Mycobacterium tuberculosis H37Rv 30S ribosomal protein S4 RpsD NP_217975.1 3878659 R 83332 CDS NP_217976.1 15610595 887524 complement(3879273..3879692) 1 NC_000962.3 Rv3459c, (MTCY13E12.12c), len: 139 aa. rpsK, 30S ribosomal protein S11, equivalent to Q9X7A0|RS11_MYCLE|RPSK|ML1959|MLCB1222.29c 30S ribosomal protein S11 from Mycobacterium leprae (138 aa), FASTA scores: opt: 819, E(): 7.6e-44, (89.95% identity in 139 aa overlap); and P45812|RS11_MYCBO 30S ribosomal protein S11 from Mycobacterium bovis (139 aa), FASTA scores: opt: 867,E(): 8.4e-47, (94.25% identity in 139 aa overlap). Also highly similar to others e.g. P72403|RS11_STRCO|SC6G4.06 from Streptomyces coelicolor (134 aa), FASTA scores: opt: 729, E(): 2.6e-38, (79.85% identity in 139 aa overlap); O50633|RS11_BACHD|RPSK|BH0161 from Bacillus halodurans (129 aa), FASTA scores: opt: 618, E(): 1.7e-31, (70.3% identity in 128 aa overlap); P04969|RS11_BACSU|RPSK from Bacillus subtilis (131 aa), FASTA scores: opt: 601, E(): 2e-30,(69.0% identity in 129 aa overlap); etc. Contains ribosomal protein S11 signature (PS00054). Belongs to the S11P family of ribosomal proteins.; 30S ribosomal protein S11 RpsK 3879692 rpsK 887524 rpsK Mycobacterium tuberculosis H37Rv 30S ribosomal protein S11 RpsK NP_217976.1 3879273 R 83332 CDS NP_217977.1 15610596 887514 complement(3879696..3880070) 1 NC_000962.3 Rv3460c, (MTCY13E12.13c), len: 124 aa. rpsM, 30S ribosomal protein S13, equivalent to Q9X7A1|RS13_MYCLE|RPSM|ML1960|MLCB1222.30c 30S ribosomal protein S13 from Mycobacterium leprae (124 aa), FASTA scores: opt: 762, E(): 1.5e-43, (92.75% identity in 124 aa overlap); and P45813|RS13_MYCBO|RPSM from Mycobacterium bovis (123 aa), FASTA scores: opt: 727, E(): 3e-41, (98.25% identity in 114 aa overlap). Also highly similar to others e.g. O86773|RS13_STRCO|SC6G4.05 from Streptomyces coelicolor (126 aa), FASTA scores: opt: 631, E(): 6.2e-35,(73.75% identity in 122 aa overlap); Q9RA65|RPS13 from Thermus aquaticus (subsp. thermophilus) (126 aa), FASTA scores: opt: 552, E(): 9.8e-30, (62.6% identity in 123 aa overlap); P20282|RS13_BACSU|RPSM from Bacillus subtilis (120 aa), FASTA scores: opt: 533, E(): 1.7e-28, (64245% identity in 121 aa overlap); etc. Contains ribosomal protein S13 signature (PS00646). Belongs to the S13P family of ribosomal proteins.; 30S ribosomal protein S13 RpsM 3880070 rpsM 887514 rpsM Mycobacterium tuberculosis H37Rv 30S ribosomal protein S13 RpsM NP_217977.1 3879696 R 83332 CDS NP_217978.1 15610597 887272 complement(3880286..3880399) 1 NC_000962.3 Rv3461c, (MTCY13E12.14c), len: 37 aa. rpmJ, 50S ribosomal protein L36, equivalent to P45810|RL36_MYCBO|RPMJ from Mycobacterium bovis (37 aa); and Q9X7A2|RL36_MYCLE|RPMJ|ML1961|MLCB1222.31c 50S ribosomal protein L36 from Mycobacterium leprae (37 aa), FASTA scores: opt: 241, E(): 9.7e-14, (86.5% identity in 37 aa overlap). Also highly similar to others e.g. O86772|RL36_STRCO|SC6G4.04 from Streptomyces coelicolor (37 aa), FASTA scores: opt: 233, E(): 4.5e-13, (83.8% identity in 37 aa overlap); P07841|RL36_BACST|RPMJ from Bacillus stearothermophilus (37 aa), FASTA scores: opt: 214, E(): 1.6e-11, (72.95% identity in 37 aa overlap); P12230|RK36_SPIOL|RPL36 from Spinacia oleracea (Spinach) (37 aa), FASTA scores: opt: 211, E(): 2.9e-11, (70.25% identity in 37 aa overlap); etc. Contains PS00828 Ribosomal protein L36 signature. Belongs to the L36P family of ribosomal proteins.; 50S ribosomal protein L36 RpmJ 3880399 rpmJ 887272 rpmJ Mycobacterium tuberculosis H37Rv 50S ribosomal protein L36 RpmJ NP_217978.1 3880286 R 83332 CDS NP_217979.1 15610598 887325 complement(3880432..3880653) 1 NC_000962.3 Rv3462c, (MTCY13E12.15c), len: 73 aa. Probable infA,initiation factor if-1, equivalent to P45957|ML1962|INFA translation initiation factor if-1 from Mycobacterium bovis (72 aa) and Mycobacterium leprae (72 aa), FASTA scores: opt: 472, E(): 6.6e-28, (100.0% identity in 72 aa overlap). Also highly similar to others e.g. O54209|IF1_STRCO|INFA|SC6G4.03 from Streptomyces coelicolor (73 aa), FASTA scores: opt: 424, E(): 2e-24, (84.95% identity in 73 aa overlap); O50630|IF1_BACHD|INFA|BH0158 from Bacillus halodurans (71 aa), FASTA scores: opt: 388,E(): 8.1e-22, (77.8% identity in 72 aa overlap); Q9XD14|IF1_LEPIN|INFA from Leptospira interrogans (71 aa),FASTA scores: opt: 376, E(): 6e-21, (80.0% identity in 70 aa overlap); etc. Contains 1 'S1 motif' domain. Belongs to the if-1 family.; Probable translation initiation factor if-1 InfA 3880653 infA 887325 infA Mycobacterium tuberculosis H37Rv Probable translation initiation factor if-1 InfA NP_217979.1 3880432 R 83332 CDS NP_217980.1 15610599 888286 3880907..3881764 1 NC_000962.3 Rv3463, (MTCY13E12.16), len: 285 aa. Conserved protein, similar to Q9RDA2|SCE20.23 hypothetical 31.4 KDA protein from Streptomyces coelicolor (290 aa), FASTA scores: opt: 770, E(): 2.2e-41, (48.6% identity in 247 aa overlap); and Q9X7Y1|SC6A5.35 putative oxidoreductase from Streptomyces coelicolor (341 aa), (see blastp results),FASTA scores: opt: 119, E(): 2.9, (24.1% identity in 274 aa overlap).; hypothetical protein 3881764 888286 Rv3463 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217980.1 3880907 D 83332 CDS NP_217981.1 15610600 887332 3881837..3882832 1 NC_000962.3 Rv3464, (MTCY13E12.17), len: 331 aa. RmlB (alternate gene name: rfbB), dTDP-glucose-4,6-dehydratase (see citations below), nearly identical to Q50556|RMLB rhamnose biosynthesis protein from Mycobacterium tuberculosis (329 aa) (previously rfbB, now known as rmlB). Equivalent to Q9CBH7|RMLB|ML1964 dTDP-glucose 4,6-dehydratase (alias Q9X7A3|RMLB putative dTDP-(glucose or rhamnose)-4,6-dehydratase (331 aa)) from Mycobacterium leprae (333 aa), FASTA scores: opt: 1925, E(): 1.9e-112,(84.0% identity in 331 aa overlap). Also highly similar to others e.g. Q9UZH2|RFBB|PAB0785 from Pyrococcus abyssi (333 aa), FASTA scores: opt: 1115, E(): 4.2e-62, (51.55% identity in 322 aa overlap); O27817|MTH1789 from Methanobacterium thermoautotrophicum (336 aa), FASTA scores: opt: 1104, E(): 2.1e-61, (51.65% identity in 331 aa overlap); BAB60064|TVG0950610 from Thermoplasma volcanium (318 aa), FASTA scores: opt: 1102, E(): 2.6e-61, (49.65% identity in 310 aa overlap); etc. Also related to P72050|MTCY13D12.18|RV3784 hypothetical 36.3 KDA protein (similar to galactowaldenases from eukaryotic and prokaryotic origin) from Mycobacterium tuberculosis (326 aa), FASTA scores: E(): 1.4e-26, (33.8% identity in 320 aa overlap).; dTDP-glucose 4,6-dehydratase RmlB 3882832 rmlB 887332 rmlB Mycobacterium tuberculosis H37Rv dTDP-glucose 4,6-dehydratase RmlB NP_217981.1 3881837 D 83332 CDS NP_217982.1 15610601 887352 3882834..3883442 1 NC_000962.3 Rv3465, (MTCY13E12.18), len: 202 aa. RmlC (alternate gene name: rfbC), dTDP-4-dehydrorhamnose 3,5-epimerase (see citations below), nearly identical to O33170|RMLC RMLC protein from Mycobacterium tuberculosis (203 aa), FASTA scores: opt: 1171, E(): 2.6e-71, (89.5% identity in 200 aa overlap) (previously known as rfbC). Equivalent to Q9X7A4|RMLC|ML1965 putative dTDP-4-dehydrorhamnose 3,5-epimerase from Mycobacterium leprae (202 aa), FASTA scores: opt: 1072, E(): 1.1e-64, (75.4% identity in 199 aa overlap). Also highly similar to others e.g. Q9F8S7|CUMY from Streptomyces rishiriensis (198 aa), FASTA scores: opt: 671, E(): 7e-38, (51.3% identity in 193 aa overlap); Q9L6C5 from Streptomyces antibioticus (202 aa), FASTA scores: opt: 665, E(): 1.8e-37, (49.25% identity in 197 aa overlap); P29783|STRM_STRGR from Streptomyces griseus (200 aa), FASTA scores: opt: 608, E(): 1.2e-33, (49.25% identity in 201 aa overlap); Q54265|STRM from Streptomyces glaucescens (200 aa), FASTA scores: opt: 603, E(): 2.5e-33, (46.7% identity in 197 aa overlap); etc. Also highly similar to Q9S4D4|TYLJ putative NDP-hexose 3-epimerase from Streptomyces fradiae (205 aa), FASTA scores: opt: 625, E(): 8.6e-35, (45.9% identity in 194 aa overlap).; dTDP-4-dehydrorhamnose 3,5-epimerase RmlC (dTDP-4-keto-6-deoxyglucose 3,5-epimerase) (dTDP-L-rhamnose synthetase) (thymidine diphospho-4-keto-rhamnose 3,5-epimerase) 3883442 rmlC 887352 rmlC Mycobacterium tuberculosis H37Rv dTDP-4-dehydrorhamnose 3,5-epimerase RmlC (dTDP-4-keto-6-deoxyglucose 3,5-epimerase) (dTDP-L-rhamnose synthetase) (thymidine diphospho-4-keto-rhamnose 3,5-epimerase) NP_217982.1 3882834 D 83332 CDS NP_217983.1 15610602 887830 3883525..3884193 1 NC_000962.3 Rv3466, (MTCY13E12.19), len: 222 aa. Conserved hypothetical ORF in REP13E12 repeat, but extending 5' of repeat. Has segment of identity to other REP13E12 ORF's e.g. MTCY336.16, MTCI65.15c, MTCY09F9.19, cMTCY251.14c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3884193 887830 Rv3466 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217983.1 3883525 D 83332 CDS NP_217984.1 15610603 888022 3883964..3884917 1 NC_000962.3 Rv3467, (MTCY13E12.20), len: 317 aa. Conserved hypothetical ORF in REP13E12 repeat, identical to ORF's from other REP13E12 copies e.g. MTCY251.13c, MTCI65.15c,MTCY09F9.19, cMTCY336.17. Also identical to Mycobacterium bovis Q50655 hypothetical 34.6 kDa protein (317 aa) in identical repeat.; hypothetical protein 3884917 888022 Rv3467 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217984.1 3883964 D 83332 CDS YP_177974.1 57117109 888007 complement(3884975..3886069) 1 NC_000962.3 Rv3468c, (MTCY13E12.21c), len: 364 aa. Possible dTDP-glucose-4,6-dehydratase, but experimental study shown that the purified protein didn't have dTDP-glucose dehydratase (rmlB) activity (see Ma et al., 2001). Similar to others e.g. O08246|MTME from Streptomyces argillaceus (331 aa), FASTA scores: opt: 238, E(): 1.2e-07, (29.65% identity in 344 aa overlap); Q9LFG7|F4P12_220 from Arabidopsis thaliana (Mouse-ear cress) (433 aa), FASTA scores: opt: 237, E(): 1.8e-07, (27.25% identity in 308 aa overlap); Q9LZI2|F26K9_260 from Arabidopsis thaliana (Mouse-ear cress) (445 aa), FASTA scores: opt: 225, E(): 1e-06, (25.95% identity in 335 aa overlap); etc. Also similar to various enzymes and hypothetical unknowns proteins e.g. BAB48655|MLL1234 UDP-glucose 4-epimerase from Rhizobium loti (Mesorhizobium loti) (307 aa), FASTA scores: opt: 757, E(): 4.6e-40, (43.4% identity in 302 aa overlap). First start taken, alternative at 17080 in cSCYY13E12 suggested by similarity. Note that previously known as rmlB3 (see Ma et al., 2001).; Possible dTDP-glucose 4,6-dehydratase 3886069 888007 Rv3468c Mycobacterium tuberculosis H37Rv Possible dTDP-glucose 4,6-dehydratase YP_177974.1 3884975 R 83332 CDS NP_217986.1 15610605 888074 complement(3886073..3887083) 1 NC_000962.3 Rv3469c, (MTCY13E12.22c), len: 336 aa. Probable mhpE, 4-hydroxy-2-oxovalerate aldolase, similar to others (principally from Pseudomonas species) e.g. Q99PZ1|SCP1.301|SCP1.53c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 615, E(): 7.9e-31, (37.65% identity in 332 aa overlap); Q9X9Q0|NIKB NIKB protein (see Bruntner et al., 1999) from Streptomyces tendae (357 aa), FASTA scores: opt: 571, E(): 4.4e-28, (34.5% identity in 339 aa overlap); P51014|BPHF_PSES1 from Pseudomonas sp. strain KKS102 (352 aa), FASTA scores: opt: 549, E(): 9.9e-27,(31.2% identity in 314 aa overlap); Q51983|CMTG_PSEPU from Pseudomonas putida (350 aa), FASTA scores: opt: 543, E(): 2.3e-26, (30.7% identity in 319 aa overlap); P51020|MHPE_ECOLI|MHPF|B0352 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 517, E(): 9.1e-25, (31.75% identity in 312 aa overlap); etc. Also similar to P71867|MTCY03C7.22|Rv3534c hypothetical 36.4 KDA protein from Mycobacterium tuberculosis (346 aa), FASTA scores: E(): 7.5e-24, (31.9% identity in 310 aa overlap).; Probable 4-hydroxy-2-oxovalerate aldolase MhpE (HOA) 3887083 mhpE 888074 mhpE Mycobacterium tuberculosis H37Rv Probable 4-hydroxy-2-oxovalerate aldolase MhpE (HOA) NP_217986.1 3886073 R 83332 CDS NP_217987.1 15610606 888041 complement(3887144..3888802) 1 NC_000962.3 Rv3470c, (MTCY13E12.23c), len: 552 aa. Probable ilvB2, acetolactate synthase large subunit, similar to others e.g. P73913|ILVG|SLR2088 from Synechocystis sp. strain PCC 6803 (621 aa), FASTA scores: opt: 779, E(): 4.5e-39, (30.7% identity in 567 aa overlap); O78518|ILVB_GUITH from Guillardia theta (Cryptomonas phi) (575 aa), FASTA scores: opt: 742, E(): 6.9e-37, (28.8% identity in 566 aa overlap); Q59950|ILVX from Spirulina platensis (612 aa), FASTA scores: opt: 715, E(): 3e-35,(28.45% identity in 569 aa overlap); etc. Contains thiamine pyrophosphate enzymes signature (PS00187).; Probable acetolactate synthase (large subunit) IlvB2 (AHAS) (acetohydroxy-acid synthase large subunit) (ALS) 3888802 ilvB2 888041 ilvB2 Mycobacterium tuberculosis H37Rv Probable acetolactate synthase (large subunit) IlvB2 (AHAS) (acetohydroxy-acid synthase large subunit) (ALS) NP_217987.1 3887144 R 83332 CDS NP_217988.1 15610607 888050 complement(3888808..3889341) 1 NC_000962.3 Rv3471c, (MTCY13E12.24c), len: 177 aa. Conserved hypothetical protein, similar to Q59013|MJ1618 hypothetical protein from Methanococcus jannaschii (125 aa), FASTA scores: opt: 262, E(): 1.2e-09, (39.05% identity in 105 aa overlap); and O26452|MTH352 conserved protein from Methanobacterium thermoautotrophicum (131 aa), FASTA scores: opt: 222, E(): 3.8e-07, (35.05% identity in 117 aa overlap). Equivalent to AAK47934 from Mycobacterium tuberculosis strain CDC1551 (184 aa) but shorter 7 aa.; hypothetical protein 3889341 888050 Rv3471c Mycobacterium tuberculosis H37Rv hypothetical protein NP_217988.1 3888808 R 83332 CDS NP_217989.1 15610608 888006 3889362..3889868 1 NC_000962.3 Rv3472, (MTCY13E12.25), len: 168 aa. Conserved protein, showing some similarity to other proteins e.g. Q9ZAT9|DPSH daunorubicin biosynthesis enzyme from Streptomyces peucetius (194 aa), FASTA scores: opt: 181,E(): 6.8e-05, (30.7% identity in 127 aa overlap); Q53879 DAUH/E from Streptomyces sp. C5 (151 aa), FASTA scores: opt: 168, E(): 0.00038, (29.25% identity in 127 aa overlap); and Q9L4U3|AKNV from Streptomyces galilaeus (144 aa), FASTA scores: opt: 122, E(): 0.36, (31.25% identity in 129 aa overlap).; hypothetical protein 3889868 888006 Rv3472 Mycobacterium tuberculosis H37Rv hypothetical protein NP_217989.1 3889362 D 83332 CDS NP_217990.1 15610609 888101 complement(3889948..3890733) 1 NC_000962.3 Rv3473c, (MTCY13E12.26c), len: 261 aa. Possible bpoA, peroxidase (non-haem peroxidase), similar to various enzymes or hypothetical unknown proteins e.g. O85849 hypothetical 26.2 KDA protein from Sphingomonas aromaticivorans (247 aa), FASTA scores: opt: 684, E(): 4.9e-34, (43.8% identity in 242 aa overlap); AAK45412|MT1155 hydrolase, alpha/beta hydrolase fold family from Mycobacterium tuberculosis strain CDC1551 (311 aa),FASTA scores: opt: 675, E(): 2e-33, (39.45% identity in 256 aa overlap); Q9K3V0|SCD10.27 putative hydrolase from Streptomyces coelicolor (352 aa), FASTA scores: opt: 248,E(): 9.7e-08, (26.05% identity in 261 aa overlap); P29715|BPA2_STRAU|BPOA2 non-haem bromoperoxidase (bromide peroxidase) (277 aa), FASTA scores: opt: 237, E(): 3.6e-07,(29.45% identity in 265 aa overlap); O31168|PRXC_STRAU|CPO|CPOT non-heme chloroperoxidase (278 aa), FASTA scores: opt: 236, E(): 4.2e-07, (29.45% identity in 265 aa overlap); AAK62388|T5L19.180 lipase-like protein from Arabidopsis thaliana (Mouse-ear cress) (350 aa), FASTA scores: opt: 236, E(): 5.1e-07, (26.65% identity in 274 aa overlap); etc. Also similar to O06575|BPOB|Rv1123c|MTCY22G8.12c hypothetical 32.5 KDA protein from Mycobacterium tuberculosis (302 aa), FASTA scores: opt: 675, E(): 2e-33, (39.45% identity in 256 aa overlap). Equivalent to AAK47936 from Mycobacterium tuberculosis strain CDC1551 (294 aa) but shorter 33 aa. May have been inactivated or truncated by neighbouring IS6110.; Possible peroxidase BpoA (non-haem peroxidase) 3890733 bpoA 888101 bpoA Mycobacterium tuberculosis H37Rv Possible peroxidase BpoA (non-haem peroxidase) NP_217990.1 3889948 R 83332 CDS NP_217991.1 15610610 888097 3890830..3891156 1 NC_000962.3 Rv3474, (MTCY13E12.27), len: 108 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3474 and Rv3475, the sequence UUUUAAAG (directly upstream of Rv3475) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Belongs to the transposase family 8.; Possible transposase for insertion element IS6110 (fragment) 3891156 888097 Rv3474 Mycobacterium tuberculosis H37Rv Possible transposase for insertion element IS6110 (fragment) NP_217991.1 3890830 D 83332 CDS NP_217992.2 448824814 888055 <3891105..3892091 1 NC_000962.3 Rv3475, (MTCY13E12.28), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3474 and Rv3475, the sequence UUUUAAAG (directly upstream of Rv3475) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (- 18 aa).; Possible transposase for insertion element IS6110 [second part] 3892091 888055 Rv3475 Mycobacterium tuberculosis H37Rv Possible transposase for insertion element IS6110 [second part] NP_217992.2 <3891105 D 83332 CDS NP_217993.1 15610612 888002 complement(3892371..3893720) 1 NC_000962.3 Rv3476c, (MTCY13E12.29c), len: 449 aa. Probable kgtP, dicarboxylate-transport integral membrane protein,possibly member of major facilitator superfamily (MFS),highly similar to others e.g. Q9HT43|PA5530 from Pseudomonas aeruginosa (435 aa), FASTA scores: opt: 1209,E(): 2.3e-68, (47.05% identity in 425 aa overlap); Q9I6Q9|PCAT|PA0229 from Pseudomonas aeruginosa (432 aa),FASTA scores: opt: 1131, E(): 1.8e-63, (40.4% identity in 438 aa overlap); Q9WWZ2 from Pseudomonas putida (429 aa),FASTA scores: opt: 1090, E(): 6.5e-61, (41.2% identity in 425 aa overlap); P17448|KGTP_ECOLI|WITA|B2587 from Escherichia coli strain K12 (432 aa), FASTA scores: opt: 1083, E(): 1.8e-60, (40.05% identity in 422 aa overlap); etc. Also similar to O05301|MTCI364.12|Rv1200 hypothetical 44.6 KDA protein from Mycobacterium tuberculosis (425 aa),FASTA scores: E(): 5.2e-25, (28.5% identity in 382 aa overlap). Contains sugar transport protein signatures 1 and 2 (PS00216, PS00217). Belongs to the sugar transporter family.; Probable dicarboxylic acid transport integral membrane protein KgtP (dicarboxylate transporter) 3893720 kgtP 888002 kgtP Mycobacterium tuberculosis H37Rv Probable dicarboxylic acid transport integral membrane protein KgtP (dicarboxylate transporter) NP_217993.1 3892371 R 83332 CDS YP_177975.1 57117110 888474 3894093..3894389 1 NC_000962.3 Rv3477, (MTCY13E12.30), len: 98 aa. PE31, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to O53941|Rv1791|MTV049.13 (99 aa),FASTA scores: opt: 373, E(): 4.3e-18, (64.65% identity in 99 aa overlap); MTCI364.07; MTCY21C12.10c; MTCY1A11.25c; MTC1A11.04; MTCY359.33; etc.; PE family protein PE31 3894389 PE31 888474 PE31 Mycobacterium tuberculosis H37Rv PE family protein PE31 YP_177975.1 3894093 D 83332 CDS YP_177976.1 57117111 888047 3894426..3895607 1 NC_000962.3 Rv3478, (MTCY13E12.31), len: 393 aa. PPE60 (alternate gene name: mtb39c). Member of the M. tuberculosis PPE family, highly similar to others e.g. Q11031|YD61_MYCTU|Rv1361c|MT1406|MTCY02B10.25c (396 aa),FASTA scores: opt: 2165, E(): 1.1e-109, (85.35% identity in 396 aa overlap); MTCI364.08; MTCY10G2.10; MTCY03A2.22c; MTCY274.23c; MTCY164.34c; MTCY98.0029c; etc. Note that expression of Rv3478 was demonstrated in lysates by immunodetection (see Dillon et al., 1999).; PE family protein PPE60 3895607 PPE60 888047 PPE60 Mycobacterium tuberculosis H37Rv PE family protein PPE60 YP_177976.1 3894426 D 83332 CDS NP_217996.2 57117112 888478 3895820..3898885 1 NC_000962.3 Rv3479, (MTCY13E12.32), len: 1021 aa. Possible transmembrane protein, with hydrophobic stretches at C-terminus. Start changed since first submission (-54 aa). Alternative nucleotide at position 3896340 (T->G; L174R) has been observed.; Possible transmembrane protein 3898885 888478 Rv3479 Mycobacterium tuberculosis H37Rv Possible transmembrane protein NP_217996.2 3895820 D 83332 CDS NP_217997.1 15610616 888473 complement(3898909..3900402) 1 NC_000962.3 Rv3480c, (MTCY13E12.33c), len: 497 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O69701|Y1D4_MYCTU|Rv3734c|MT3839|MTV025.082c (454 aa), FASTA scores: opt: 520, E(): 2e-23, (39.95% identity in 488 aa overlap); Q10554|Y895_MYCTU|Rv0895|MTCY31.23 (505 aa), FASTA scores: opt: 434, E(): 2.7e-18, (34.2% identity in 497 aa overlap); AAK45165|MT0919 (520 aa), FASTA scores: opt: 434, E(): 2.7e-18, (34.2% identity in 497 aa overlap); etc. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 272, E(): 1e-08, (28.85% identity in 485 aa overlap); and Q9RIU8|CM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 254,E(): 1.1e-07, (30.4% identity in 497 aa overlap). Seems to belong to the UPF0089 family.; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 3900402 888473 Rv3480c Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_217997.1 3898909 R 83332 CDS NP_217998.1 15610617 888466 complement(3900493..3901182) 1 NC_000962.3 Rv3481c, (MTCY13E12.34c), len: 229 aa. Probable integral membrane protein. No real similarity with others.; Probable integral membrane protein 3901182 888466 Rv3481c Mycobacterium tuberculosis H37Rv Probable integral membrane protein NP_217998.1 3900493 R 83332 CDS NP_217999.1 15610618 888480 complement(3901324..3902106) 1 NC_000962.3 Rv3482c, (MTCY13E12.35c), len: 260 aa. Probable conserved membrane protein. N-terminal region shares some similarity with N-terminus of O88067|SCI35.32c putative membrane protein from Streptomyces coelicolor (319 aa),FASTA scores: opt: 155, E(): 0.023, (54.55% identity in 33 aa overlap); and with C-terminus of O06254|Rv3437|MTCY77.09 hypothetical 17.9 KDA protein from Mycobacterium tuberculosis strain H37Rv (alias AAK47883|MT3542.1 from strain CDC1551) (158 aa), FASTA scores: opt: 140, E(): 0.11, (58.8% identity in 34 aa overlap). Some similarity to others e.g. Q9XAN5|SC4C6.05c putative membrane protein from Streptomyces coelicolor (347 aa), FASTA scores: opt: 131,E(): 0.75, (29.4% identity in 221 aa overlap). First start taken.; Probable conserved membrane protein 3902106 888480 Rv3482c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_217999.1 3901324 R 83332 CDS NP_218000.1 15610619 888448 complement(3902150..3902812) 1 NC_000962.3 Rv3483c, (MTCY13E12.36c), len: 220 aa. Possible exported protein, similar to Q9CC94|ML1099 putative lipoprotein from Mycobacterium leprae (202 aa), FASTA scores: opt: 276, E(): 1.4e-08, (33.1% identity in 148 aa overlap). Also showing similarity with Mycobacterium tuberculosis proteins Q11065|LPRE_MYCTU|LPRE|Rv1252c|MT1291|MTCY50.30. putative lipoprotein precursor (202 aa), FASTA scores: opt: 276,E(): 1.4e-08, (29.5% identity in 200 aa overlap); O53445|Rv1097c|MTV017.50c hypothetical 29.9 KDA protein (293 aa), FASTA scores: opt: 161, E(): 0.047, (25.4% identity in 118 aa overlap); P71882|LPPP_MYCTU|Rv2330c|MT2392|MTCY3G12.04 putative lipoprotein precursor (175 aa), FASTA scores: opt: 146,E(): 0.21, (28.25% identity in 184 aa overlap); and O06170|Rv2507|MTCY07A7.13 hypothetical 28.5 KDA protein (273 aa), FASTA scores: opt: 148, E(): 0.23, (25.15% identity in 191 aa overlap). Contains possible N-terminal signal sequence; Possible exported protein 3902812 888448 Rv3483c Mycobacterium tuberculosis H37Rv Possible exported protein NP_218000.1 3902150 R 83332 CDS NP_218001.1 15610620 888445 3903078..3904616 1 NC_000962.3 Rv3484, (MTCY13E12.37), len: 512 aa. Possible cpsA,hypothetical protein, equivalent to Q50160|CPSA|ML2247 hypothetical protein CPSA from Mycobacterium leprae (516 aa), FASTA scores: opt: 2557, E(): 1.6e-143, (74.9% identity in 518 aa overlap); and with good similarity to Q9CCK9|ML0750 hypothetical protein from Mycobacterium leprae (489 aa), FASTA scores: opt: 855, E(): 4.6e-43,(34.45% identity in 502 aa overlap). Also similar (or with similarity) to hypothetical proteins from Mycobacterium tuberculosis: P96872|Rv3267|MTCY71.07 (498 aa), FASTA scores: opt: 928, E(): 2.3e-47, (37.35% identity in 498 aa overlap); and O53834|Rv0822c|MTV043.14c (684 aa), FASTA scores: opt: 425, E(): 1.5e-17, (26.15% identity in 524 aa overlap). Shows also similarity with various bacterial proteins e.g. Q9KZK0|SCE34.26 conserved hypothetical protein from Streptomyces coelicolor (507 aa), FASTA scores: opt: 329, E(): 5.3e-12, (28.85% identity in 478 aa overlap); Q9K4E6|2SC6G5.02 conserved hypothetical protein,possible membrane protein, from Streptomyces coelicolor (382 aa), FASTA scores: opt: 305, E(): 1.1e-10, (29.8% identity in 386 aa overlap); O69850|SC1C3.08c putative transcriptional regulator from Streptomyces coelicolor (366 aa), FASTA scores: opt: 304, E(): 1.2e-10, (29.6% identity in 395 aa overlap); Q9KZK3|SCE34.23 putative transcriptional regulator from Streptomyces coelicolor (396 aa), FASTA scores: opt: 296, E(): 3.8e-10, (31.25% identity in 349 aa overlap); AAK43602|CPSA CPSA protein from Streptococcus agalactiae (485 aa), FASTA scores: opt: 250,E(): 2.4e-07, (30.25% identity in 162 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al.,2008).; Possible conserved protein CpsA 3904616 cpsA 888445 cpsA Mycobacterium tuberculosis H37Rv Possible conserved protein CpsA NP_218001.1 3903078 D 83332 CDS NP_218002.1 15610621 888427 complement(3904622..3905566) 1 NC_000962.3 Rv3485c, (MTCY13E12.38c), len: 314 aa. Probable short-chain dehydrogenase/reductase, similar, but longer 41 aa, to P71824|Rv0769|MTCY369.14 putative short-chain type dehydrogenase/reductase CY369.14 from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.0% identity in 253 aa overlap). Also similar to various dehydrogenases e.g. P25529|HDHA_ECOLI|HSDH|B1619 NAD-dependent 7 alpha-hydroxysteroid dehydrogenase (SDR family) from Escherichia coli strain K12 (alias BAB35750|ECS2327 or AAG56608|HDHA for strain O157:H7) (255 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.7% identity in 248 aa overlap); Q9FD15|RUBG putative reductase (SDR family) from Streptomyces collinus (249 aa), FASTA scores: opt: 446, E(): 1.5e-18, (36.1% identity in 255 aa overlap); BAB51974|MLL5540 putative dehydrogenase from Rhizobium loti (Mesorhizobium loti) (253 aa), FASTA scores: opt: 442, E(): 2.5e-18, (36.25% identity in 251 aa overlap); Q08632|SDR1_PICAB short-chain type dehydrogenase/reductase (SDR family) from Picea abies (Norway spruce) (Picea excelsa) (271 aa), FASTA scores: opt: 441, E(): 3.1e-18,(32.3% identity in 260 aa overlap); Q9A326|CC3380 2-deoxy-D-gluconate 3-dehydrogenase from Caulobacter crescentus (260 aa), FASTA scores: opt: 436, E(): 5.7e-18,(32.8% identity in 253 aa overlap); Q16698|DECR_HUMAN 2,4-dienoyl-CoA reductase, mitochondrial precursor from Homo sapiens (Human) (335 aa), FASTA scores: opt: 430, E(): 1.5e-17, (30.4% identity in 306 aa overlap); etc. Contains short-chain alcohol dehydrogenase family signature (PS00061). Belongs to the short-chain dehydrogenases/reductases family (SDR).; Probable short-chain type dehydrogenase/reductase 3905566 888427 Rv3485c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_218002.1 3904622 R 83332 CDS NP_218003.1 15610622 888276 3905772..3906221 1 NC_000962.3 Rv3486, (MTCY13E12.39), len: 149 aa. Conserved protein, similar to Q9RC47|YFID|BH3304 hypothetical protein from Bacillus halodurans (129 aa), FASTA scores: opt: 186,E(): 2.1e-05, (40.0% identity in 95 aa overlap); and Q9KKT1|VCA1019 hypothetical protein from Vibrio cholerae (148 aa), FASTA scores: opt: 128, E(): 0.15, (35.25% identity in 139 aa overlap). Some similarity to other proteins e.g. P54720|YFID_BACSU hypothetical protein from Bacillus subtilis (134 aa), FASTA scores: opt: 165, E(): 0.00052, (31.75% identity in 126 aa overlap). Equivalent to AAK47949 from Mycobacterium tuberculosis strain CDC1551 (163 aa) but shorter 14 aa.; hypothetical protein 3906221 888276 Rv3486 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218003.1 3905772 D 83332 CDS NP_218004.3 448824815 888430 complement(3906174..3907007) 1 NC_000962.3 Rv3487c, (MTCY13E12.41c), len: 277 aa. Probable lipF, esterase/lipase (see citation below), highly similar,but shorter 50 aa, to O53424|LIPU|Rv1076|MTV017.29 putative esterase/lipase from Mycobacterium tuberculosis (297 aa),FASTA scores: opt: 1229, E(): 3.3e-71, (76.4% identity in 246 aa overlap); and similar to other putative lipases from Mycobacterium tuberculosis e.g. P71759|LIPK|RV2385|MTCY253.36c (306 aa), FASTA scores: opt: 468, E(): 1.2e-22, (36.2% identity in 254 aa overlap). Equivalent, but shorter 79 aa, to Q9ZBM4|MLCB1450.08|ML0314 putative hydrolase (putative esterase) from Mycobacterium leprae (335 aa), FASTA scores: opt: 1225, E(): 6.6e-71,(73.6% identity in 250 aa overlap). Also similar to esterases and lipases of around 300 aa e.g. Q44087|est esterase precursor from Acinetobacter lwoffii (303 aa),FASTA scores: opt: 428, E(): 4.3e-20, (31.85% identity in 251 aa overlap); P18773|EST_ACICA esterase from Acinetobacter calcoaceticus (303 aa), FASTA scores: opt: 420, E(): 1.4e-19, (31.5% identity in 251 aa overlap); Q9KIU1 esterase from uncultured bacterium Plasmid pAH116 (308 aa), FASTA scores: opt: 405, E(): 1.3e-18, (35.1% identity in 242 aa overlap); Q9X8J4|SCE9.22 putative esterase from Streptomyces coelicolor (266 aa), FASTA scores: opt: 390, E(): 1e-17, (35.85% identity in 237 aa overlap); etc. Equivalent to AAK47950 from Mycobacterium tuberculosis strain CDC1551 (327 aa) but shorter 50 aa.; Probable esterase/lipase LipF 3907007 lipF 888430 lipF Mycobacterium tuberculosis H37Rv Probable esterase/lipase LipF NP_218004.3 3906174 R 83332 CDS NP_218005.1 15610624 888417 3907667..3907990 1 NC_000962.3 Rv3488, (MTCY13E12.41), len: 107 aa. Hypothetical protein, similar to various bacterial proteins e.g. O28730|AF1542 conserved hypothetical protein from Archaeoglobus fulgidus (101 aa), FASTA scores: opt: 321,E(): 6.4e-15, (50.55% identity in 87 aa overlap); O50207 SQ1_IV (fragment) from Rhodococcus erythropolis (59 aa),FASTA scores: opt: 298, E(): 1.4e-13, (71.2% identity in 59 aa overlap); Q9KFB0|BH0575 BH0575 protein from Bacillus halodurans (102 aa), FASTA scores: opt: 294, E(): 4.1e-13,(43.15% identity in 95 aa overlap); etc. Also similar to Mycobacterium tuberculosis P71704|Rv0047c|MTCY21D4.10c (180 aa) (37.8% identity in 82 aa overlap).; hypothetical protein 3907990 888417 Rv3488 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218005.1 3907667 D 83332 CDS NP_218006.1 15610625 888410 3908072..3908236 1 NC_000962.3 Rv3489, (MTCY13E12.42), len: 54 aa. Unknown protein. No similarity with other proteins.; hypothetical protein 3908236 888410 Rv3489 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218006.1 3908072 D 83332 CDS NP_218007.1 15610626 888404 3908236..3909738 1 NC_000962.3 Rv3490, (MTCY13E12.43), len: 500 aa. otsA, alpha,alpha-trehalose-phosphate synthase (see citations below),equivalent to Q50167|OTSA|ML2254 probable trehalose-phosphate synthase from Mycobacterium leprae (498 aa), FASTA scores: opt: 2706, E(): 1.6e-166, (80.3% identity in 497 aa overlap). Also similar to others e.g. Q92410|TPS1_CANAL from Candida albicans (Yeast) (478 aa),FASTA scores: opt: 895, E(): 4.9e-50, (37.15% identity in 479 aa overlap); Q00764|TPS1_YEASTTPS1|CIF1|BYP1|FDP1|GGS1|GLC6|YBR126c|YBR 0922 from Saccharomyces cerevisiae (Baker's yeast) (495 aa),FASTA scores: opt: 847, E(): 6.2e-47, (36.1% identity in 490 aa overlap); BAB48232|MLL0691 from Rhizobium loti (Mesorhizobium loti) (520 aa), FASTA scores: opt: 884, E(): 2.7e-49, (36.2% identity in 478 aa overlap); etc. Equivalent to AAK47953 from Mycobacterium tuberculosis strain CDC1551 (478 aa) but longer 22 aa.; Alpha, alpha-trehalose-phosphate synthase [UDP-forming] OtsA (trehalose-6-phosphate synthase) (UDP-glucose-glucosephosphate glucosyltransferase) (trehalosephosphate-UDP glucosyltransferase) (trehalose-6-phosphate synthetase) (trehalose-phosphate synthase) (trehalose-phosphate synthetase) (transglucosylase) (trehalosephosphate-UDP glucosyl transferase) 3909738 otsA 888404 otsA Mycobacterium tuberculosis H37Rv Alpha, alpha-trehalose-phosphate synthase [UDP-forming] OtsA (trehalose-6-phosphate synthase) (UDP-glucose-glucosephosphate glucosyltransferase) (trehalosephosphate-UDP glucosyltransferase) (trehalose-6-phosphate synthetase) (trehalose-phosphate synthase) (trehalose-phosphate synthetase) (transglucosylase) (trehalosephosphate-UDP glucosyl transferase) NP_218007.1 3908236 D 83332 CDS NP_218008.1 15610627 888382 3909890..3910468 1 NC_000962.3 Rv3491, (MTCY13E12.44), len: 192 aa. Unknown protein. No significant homology with other proteins.; hypothetical protein 3910468 888382 Rv3491 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218008.1 3909890 D 83332 CDS NP_218009.1 15610628 888381 complement(3910465..3910947) 1 NC_000962.3 Rv3492c, (MTCY13E12.45c), len: 160 aa. Conserved hypothetical Mce-associated protein, showing some similarity to hypothetical Mycobacterium tuberculosis proteins e.g. O53974|Rv1973|MTV051.11 (near Mce operon 3) (160 aa), FASTA scores: opt: 214, E(): 2.6e-07, (25.3% identity in 154 aa overlap); and Q11032|YD62_MYCTU|Rv1362c|MT1407|MTCY02B10.26c (220 aa),FASTA scores: opt: 187, E(): 2e-05, (23.4% identity in 154 aa overlap). Contains lipocalin signature at C-terminus (PS00213). Predicted to be an outer membrane protein (See Song et al., 2008).; Conserved hypothetical Mce associated protein 3910947 888381 Rv3492c Mycobacterium tuberculosis H37Rv Conserved hypothetical Mce associated protein NP_218009.1 3910465 R 83332 CDS NP_218010.1 15610629 888379 complement(3910947..3911675) 1 NC_000962.3 Rv3493c, (MTCY13E12.46c), len: 242 aa. Conserved hypothetical Mce-associated ala-, val-rich protein, showing weak similarity to O07422|Z97050|Rv0178|MTCI28.18 hypothetical 25.9 KDA protein (near Mce operon1) from Mycobacterium tuberculosis (244 aa), FASTA scores: opt: 163, E(): 0.046, (24.65% identity in 211 aa overlap).; Conserved hypothetical Mce associated alanine and valine rich protein 3911675 888379 Rv3493c Mycobacterium tuberculosis H37Rv Conserved hypothetical Mce associated alanine and valine rich protein NP_218010.1 3910947 R 83332 CDS NP_218011.1 15610630 888376 complement(3911675..3913369) 1 NC_000962.3 Rv3494c, (MTV023.01c), len: 564 aa. Mce4F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa); O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); and O53972|Rv1971|MTV051.09|mce3F (437 aa). Also similar to others e.g. Q9CD09|MCE1F|ML2594 putative secreted protein from Mycobacterium leprae (516 aa), FASTA scores: opt: 1040, E(): 3.6e-31, (35.9% identity in 529 aa overlap); Q9F361|SC8A2.02c putative secreted protein from Streptomyces coelicolor (433 aa), FASTA scores: opt: 570,E(): 3.7e-14, (30.8% identity in 458 aa overlap); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce4F 3913369 mce4F 888376 mce4F Mycobacterium tuberculosis H37Rv Mce-family protein Mce4F NP_218011.1 3911675 R 83332 CDS NP_218012.1 15610631 888364 complement(3913380..3914534) 1 NC_000962.3 Rv3495c, (MTV023.02c), len: 384 aa. Possible lprN (alternate gene name: mce4E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); and O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa). Also similar to others e.g. Q9F360|SC8A2.03c putative secreted protein from Streptomyces coelicolor (413 aa), FASTA scores: opt: 656, E(): 2.2e-32, (37.55% identity in 317 aa overlap); Q9CD10|LPRK|ML2593 putative lipoprotein from Mycobacterium leprae (392 aa), FASTA scores: opt: 616, E(): 5.5e-30, (28.95% identity in 373 aa overlap); etc. Contains possible signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Possible Mce-family lipoprotein LprN (Mce-family lipoprotein Mce4E) 3914534 lprN 888364 lprN Mycobacterium tuberculosis H37Rv Possible Mce-family lipoprotein LprN (Mce-family lipoprotein Mce4E) NP_218012.1 3913380 R 83332 CDS NP_218013.1 15610632 888361 complement(3914531..3915886) 1 NC_000962.3 Rv3496c, (MTV023.03c), len: 451 aa. Mce4D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); and O53970|Rv1969|MTV051.07|mce3D (423 aa). Also similar to others e.g. Q9CD11|MCE1D|ML2592 putative secreted protein from Mycobacterium leprae (531 aa), FASTA scores: opt: 837,E(): 2.6e-34, (34.55% identity in 446 aa overlap); Q9F359|SC8A2.04c putative secreted protein from Streptomyces coelicolor (337 aa), FASTA scores: opt: 606,E(): 4.9e-23, (32.35% identity in 300 aa overlap); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce4D 3915886 mce4D 888361 mce4D Mycobacterium tuberculosis H37Rv Mce-family protein Mce4D NP_218013.1 3914531 R 83332 CDS NP_218014.1 15610633 888354 complement(3915883..3916956) 1 NC_000962.3 Rv3497c, (MTV023.04c), len: 357 aa. Mce4C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); and O53969|Rv1968|MTV051.06|mce3C (410 aa). Also similar to others e.g. Q9F358|SC8A2.05c putative secreted protein from Streptomyces coelicolor (351 aa), FASTA scores: opt: 658,E(): 1.1e-30, (33.95% identity in 318 aa overlap); Q9CD12|MCE1C|ML2591 putative secreted protein from Mycobacterium leprae (519 aa), FASTA scores: opt: 555, E(): 1.2e-24, (28.35% identity in 328 aa overlap); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce4C 3916956 mce4C 888354 mce4C Mycobacterium tuberculosis H37Rv Mce-family protein Mce4C NP_218014.1 3915883 R 83332 CDS NP_218015.1 15610634 888349 complement(3916946..3917998) 1 NC_000962.3 Rv3498c, (MTV023.05c), len: 350 aa. Mce4B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); and O53968|Rv1967|MTV051.05|mce3B (342 aa). Also similar to others e.g. Q9CD13|MCE1B|ML2590 putative secreted protein from Mycobacterium leprae (346 aa), FASTA scores: opt: 803,E(): 6.1e-41, (41.05% identity in 346 aa overlap); Q9F357|SC8A2.06c putative secreted protein from Streptomyces coelicolor (354 aa), FASTA scores: opt: 624,E(): 3.4e-30, (32.55% identity in 338 aa overlap); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce4B 3917998 mce4B 888349 mce4B Mycobacterium tuberculosis H37Rv Mce-family protein Mce4B NP_218015.1 3916946 R 83332 CDS YP_177977.1 57117113 888344 complement(3917998..3919200) 1 NC_000962.3 Rv3499c, (MTV023.06c), len: 400 aa. Mce4A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); and O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa). Also similar to others e.g. Q9F356|SC8A2.07c putative secreted protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 619, E(): 7.8e-30, (32.4% identity in 352 aa overlap); Q9S4U5|MCE1 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa), FASTA scores: opt: 529,E(): 2.1e-24, (30.35% identity in 448 aa overlap); Q9CD14|MCE1A|ML2589 from Mycobacterium leprae (441 aa),FASTA scores: opt: 515, E(): 1.4e-23, (28.35% identity in 430 aa overlap); etc. Contains a possible N-terminal signal sequence. Note that previously known as mce4. Predicted to be an outer membrane protein (See Song et al., 2008).; Mce-family protein Mce4A 3919200 mce4A 888344 mce4A Mycobacterium tuberculosis H37Rv Mce-family protein Mce4A YP_177977.1 3917998 R 83332 CDS NP_218017.1 15610636 888336 complement(3919220..3920062) 1 NC_000962.3 Rv3500c, (MTV023.07c), len: 280 aa. YrbE4B,conserved integral membrane protein, part of mce4 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa); O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); and O53966|Rv1965|MTV051.03|yrbE3B (271 aa). Also highly similar to conserved hypothetical integral membrane proteins of the P45030|YRBE_HAEIN (261 aa) type, e.g. Q9CD15|YRBE1B|ML2588 from Mycobacterium leprae (289 aa),FASTA scores: opt: 973, E(): 1.5e-50, (50.2% identity in 269 aa overlap); P45030|YRBE_HAEIN|HI1086 from Haemophilus influenzae (261 aa), FASTA scores: opt: 270, E(): 6e-11,(25.4% identity in 264 aa overlap); etc.; Conserved integral membrane protein YrbE4B Possible ABC transporter 3920062 yrbE4B 888336 yrbE4B Mycobacterium tuberculosis H37Rv Conserved integral membrane protein YrbE4B Possible ABC transporter NP_218017.1 3919220 R 83332 CDS NP_218018.1 15610637 888320 complement(3920097..3920861) 1 NC_000962.3 Rv3501c, (MTV023.08c), len: 254 aa. YrbE4A,conserved integral membrane protein, part of mce4 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa); O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa); and O53965|Rv1964|MTV051.02|yrbE3A (265 aa). Also highly similar to conserved hypothetical integral membrane proteins of the P45030|YRBE_HAEIN (261 aa) type, e.g. Q9CD16|YRBE1A|ML2587 from Mycobacterium leprae (267 aa),FASTA scores: opt: 1059, E(): 1e-57, (64.75% identity in 247 aa overlap); P45030|YRBE_HAEIN|HI1086 from Haemophilus influenzae (261 aa), FASTA scores: opt: 313, E(): 3e-14,(25.7% identity in 241 aa overlap); etc.; Conserved integral membrane protein YrbE4A Possible ABC transporter 3920861 yrbE4A 888320 yrbE4A Mycobacterium tuberculosis H37Rv Conserved integral membrane protein YrbE4A Possible ABC transporter NP_218018.1 3920097 R 83332 CDS NP_218019.1 15610638 887697 complement(3921087..3922040) 1 NC_000962.3 Rv3502c, (MTV023.09c), len: 317 aa (start uncertain). Probable short-chain dehydrogenase/reductase ,similar to Mycobacterium tuberculosis proteins P71853|Rv3548c|MTCY03C7.08 hypothetical 31.1 KDA protein (304 aa), FASTA scores: opt: 739, E(): 6.2e-35, (45.15% identity in 310 aa overlap); and Q11020|YD50_MYCTU|FABG2|Rv1350|MT1393|MTCY02B10.14 putative oxidoreductase (247 aa), FASTA scores: opt: 475, E(): 5.1e-20, (40.15% identity in 254 aa overlap). Also similar to various dehydrogenases e.g. Q9I4V1|PA1023 probable short-chain dehydrogenase from Pseudomonas aeruginosa (305 aa), FASTA scores: opt: 535, E(): 2.3e-23, (37.1% identity in 302 aa overlap); Q9UVH9|FOX2 FOX2 protein (SDR family) (1015 aa), FASTA scores: opt: 487, E(): 3.2e-20, (38.4% identity in 276 aa overlap); P22414|FOX2_CANTR peroxisomal hydratase-dehydrogenase, D-3-hydroxyacyl CoA dehydrogenase (SDR family) from Candida tropicalis (Yeast) (906 aa) FASTA scores: opt: 481, E(): 6.4e-20, (38.0% identity in 250 aa overlap); P50171|DHB8_MOUSE|HSD17B8|HKE6|H2-KE6 estradiol 17 beta-dehydrogenase 8 from Mus musculus (Mouse) (260 aa) FASTA scores: opt: 459, E(): 4.3e-19, (39.75% identity in 259 aa overlap); CAC41362|BKR1 3-oxyacyl-[acyl-carrier protein] reductase (fragment) from Brassica napus (Rape) (317 aa), FASTA scores: opt: 447, E(): 2.4e-18, (39.2% identity in 255 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase Possible 17-beta-hydroxysteroid dehydrogenase 3922040 887697 Rv3502c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase Possible 17-beta-hydroxysteroid dehydrogenase NP_218019.1 3921087 R 83332 CDS NP_218020.1 15610639 888311 complement(3922065..3922256) 1 NC_000962.3 Rv3503c, (MTV023.10c), len: 63 aa. Probable fdxD,ferredoxin, equivalent to Q9R6Z5|B229_C3_226 hypothetical 9.3 KDA protein from Mycobacterium leprae (83 aa) FASTA scores: opt: 276, E(): 1.8e-13, (75.9% identity in 54 aa overlap). Also similar to several e.g. Q9R6Z5|PHDC from Nocardioides sp. strain KP7 (69 aa), FASTA scores: opt: 177, E(): 2.1e-06, (43.35% identity in 60 aa overlap); Q9X4X8|DITA3 dioxygenase DITA ferredoxin component from Pseudomonas abietaniphila (78 aa), FASTA scores: opt: 166,E(): 1.4e-05, (36.2% identity in 58 aa overlap); P00203|FER_MOOTH from Moorella thermoacetica (Clostridium thermoaceticum) (63 aa), FASTA scores: opt: 157, E(): 5.4e-05, (36.65% identity in 60 aa overlap); P18325|FER2_STRGO|SUBB from Streptomyces griseolus (64 aa) FASTA scores: opt: 157, E(): 5.5e-05, (39.35% identity in 61 aa overlap); etc. Belongs to the bacterial type ferredoxin family.; Probable ferredoxin FdxD 3922256 fdxD 888311 fdxD Mycobacterium tuberculosis H37Rv Probable ferredoxin FdxD NP_218020.1 3922065 R 83332 CDS NP_218021.1 15610640 887722 3922471..3923673 1 NC_000962.3 Rv3504, (MTV023.11), len: 400 aa. Probable fadE26,acyl-CoA dehydrogenase, similar to other acyl-CoA dehydrogenases from Mycobacterium tuberculosis e.g. P71858|FADE29|Rv3543c|MTCY03C7.13 (387 aa) FASTA scores: opt: 1031, E(): 7.5e-59, (46.25% identity in 402 aa overlap); and P95280|FADE17|Rv1934c|MTCY09F9.30 (409 aa),FASTA scores: opt: 617, E(): 3.1e-32, (32.6% identity in 423 aa overlap); etc. Also similar to others e.g. Q9A6G3|CC2131 from Caulobacter crescentus (403 aa) FASTA scores: opt: 710, E(): 3.2e-38, (33.4% identity in 413 aa overlap); Q9I4V2|PA1022 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 522, E(): 3.7e-26, (34.1% identity in 358 aa overlap); Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa), FASTA scores: opt: 509, E(): 2.6e-25,(34.45% identity in 363 aa overlap); etc. Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE26 3923673 fadE26 887722 fadE26 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE26 NP_218021.1 3922471 D 83332 CDS NP_218022.1 15610641 888248 3923698..3924819 1 NC_000962.3 Rv3505, (MTV023.12), len: 373 aa. Probable fadE27,acyl-CoA dehydrogenase, similar to other acyl-CoA dehydrogenases from Mycobacterium tuberculosis e.g. P71857|FADE28|Rv3544c|MTCY03C7.12 (339 aa) FASTA scores: opt: 497, E(): 1.8e-22, (30.3% identity in 343 aa overlap); and P95281|FADE18|Rv1933c|MTCY09F9.31 (363 aa) FASTA scores: opt: 421, E(): 6.4e-18, (32.35% identity in 334 aa overlap). Also similar to other e.g. Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 425,E(): 3.5e-18, (30.75% identity in 351 aa overlap); Q9RJX3|SCF37.28c from Streptomyces coelicolor (362 aa) FASTA scores: opt: 317, E(): 1e-11, (32.8% identity in 372 aa overlap); Q9L8Q3|PDTORFO from Pseudomonas stutzeri (Pseudomonas perfectomarina) (513 aa), FASTA scores: opt: 301, E(): 1.2e-10, (25.9% identity in 394 aa overlap); etc. Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE27 3924819 fadE27 888248 fadE27 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE27 NP_218022.1 3923698 D 83332 CDS NP_218023.1 15610642 888251 3924890..3926398 1 NC_000962.3 Rv3506, (MTV023.13), len: 502 aa. fadD17,fatty-acid-CoA synthetase (ligase), similar to P72007|FADD1|RV1750c|MTCY28.13c|MTCY04C12.34 from Mycobacterium tuberculosis (532 aa), FASTA scores: opt: 666, E(): 9.8e-32, (52.05% identity in 488 aa overlap). Also similar to various ligases/synthetases e.g. Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. HR167 (491 aa), FASTA scores: opt: 490, E(): 2.1e-21, (30.3% identity in 462 aa overlap); BAB33463|ECS0040 (alias AAG54340|CAIC) probable crotonobetaine/carnitine-CoA ligase from Escherichia coli strain O157:H7 (522 aa), FASTA scores: opt: 478, E(): 1.1e-20, (28.5% identity in 347 aa overlap); Q9KHL1|ENCH putative acyl-CoA ligase from Streptomyces maritimus (535 aa), FASTA scores: opt: 477, E(): 1.3e-20,(28.7% identity in 453 aa overlap); Q50017|XCLC|ML1051 acyl-CoA synthase from Mycobacterium leprae (476 aa), FASTA scores: opt: 472, E(): 2.3e-20, (31.35% identity in 469 aa overlap); P31552|CAIC_ECOLI|B0037 from Escherichia coli strain K12 (522 aa), FASTA scores: opt: 467, E(): 4.8e-20,(28.75% identity in 348 aa overlap); Q9KBC2|BH2006 from Bacillus halodurans long-chain acyl-CoA synthetase (ligase) (513 aa), FASTA scores: opt: 462, E(): 9.4e-20, (27.65% identity in 463 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature.; Fatty-acid-CoA synthetase FadD17 (fatty-acid-CoA synthase) (fatty-acid-CoA ligase) 3926398 fadD17 888251 fadD17 Mycobacterium tuberculosis H37Rv Fatty-acid-CoA synthetase FadD17 (fatty-acid-CoA synthase) (fatty-acid-CoA ligase) NP_218023.1 3924890 D 83332 CDS YP_177978.1 57117114 888256 3926569..3930714 1 NC_000962.3 Rv3507, (MTV023.14), len: 1381 aa. PE_PGRS53, Member of the Mycobacterium tuberculosis PE protein family, PGRS subfamily of gly-rich proteins (see citation below),similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O06810|Rv1450c|MTCY493.04 (1329 aa),FASTA scores: opt: 2173, E(): 1.4e-135, (51.15% identity in 1412 aa overlap). Equivalent to AAK47970 from Mycobacterium tuberculosis strain CDC1551 (1384 aa) but with some minor differences between the proteins. Contains two PS00583 pfkB family of carbohydrate kinases signatures 1.; PE-PGRS family protein PE_PGRS53 3930714 PE_PGRS53 888256 PE_PGRS53 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS53 YP_177978.1 3926569 D 83332 CDS YP_177979.1 57117115 888270 3931005..3936710 1 NC_000962.3 Rv3508, (MTV023.15), len: 1901 aa. PE_PGRS54, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. downstream O53559|Rv3514|MTV023.21 (1489 aa),FASTA scores: opt: 6598, E(): 0, (71.05% identity in 1533 aa overlap). Equivalent to AAK47971 from Mycobacterium tuberculosis strain CDC1551 (1384 aa) but shorter 13 aa and with some minor differences between the proteins. Contains five PS00583 pfkB family of carbohydrate kinases signatures 1.; PE-PGRS family protein PE_PGRS54 3936710 PE_PGRS54 888270 PE_PGRS54 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS54 YP_177979.1 3931005 D 83332 CDS NP_218026.1 15610645 888267 complement(3936877..3938424) 1 NC_000962.3 Rv3509c, (MTV023.16), len: 515 aa. Probable ilvX,acetohydroxyacid synthase, equivalent to Mycobacterium leprae protein described as Acetolactate synthase I, valine sensitive, large subunit Q49865|ILVX|ILVI1|B229_C3_222 (515 aa), FASTA scores: opt: 2762, E(): 8.8e-145, (82.9% identity in 515 aa overlap). Also similar to various enzymes (principally acetohydroxyacid/acetolactate synthases) e.g. Q9AB41|CC0393 thiamine-pyrophosphate-requiring enzyme from Caulobacter crescentus (512 aa), FASTA scores: opt: 1572, E(): 2.8e-79,(50.95% identity in 514 aa overlap); BAB50432|MLL3567 acetolactate synthase I from Rhizobium loti (Mesorhizobium loti) (517 aa), FASTA scores: opt: 1440, E(): 5.2e-72,(47.9% identity in 548 aa overlap); P20906|MDLC_PSEPU benzoylformate decarboxylase from Pseudomonas putida (528 aa), FASTA scores: opt: 356, E(): 2.5e-12, (28.1% identity in 530 aa overlap); Q9L123|SC6D11.33c putative decarboxylase from Streptomyces coelicolor (526 aa), FASTA scores: opt: 325, E(): 1.3e-10, (33.2% identity in 530 aa overlap); Q9RDF9|SCC57A.40c putative acetolactate synthase from Streptomyces coelicolor (564 aa), FASTA scores: opt: 304, E(): 1.9e-09, (28.55% identity in 550 aa overlap); P94783 valine-sensitive acetohydroxy acid synthase from Citrobacter freundii (561 aa), FASTA scores: opt: 278, E(): 5.1e-08, (25.8% identity in 550 aa overlap); Q42767|AHAS acetohydroxyacid synthase from Gossypium hirsutum (Upland cotton) (659 aa), FASTA scores: opt: 278, E(): 5.8e-08,(26.15% identity in 558 aa overlap); etc. Note that other Mycobacterium tuberculosis proteins, e.g. O53250|MTV012.17c|ILVB_MYCTU|Rv3003c|MT3083|MTV012.17c, showbetter similarity to Acetolactate synthase I. Similar to other enzymes which require TPP. Cofactor: thiamin pyrophosphate (by similarity).; Probable acetohydroxyacid synthase IlvX (acetolactate synthase) 3938424 ilvX 888267 ilvX Mycobacterium tuberculosis H37Rv Probable acetohydroxyacid synthase IlvX (acetolactate synthase) NP_218026.1 3936877 R 83332 CDS NP_218027.1 15610646 888298 complement(3938421..3939257) 1 NC_000962.3 Rv3510c, (MTV023.17), len: 278 aa. Conserved protein, similar to Q50662|Rv2303c|MTCY339.06 hypothetical 34.6 KDA protein from Mycobacterium tuberculosis (307 aa),FASTA scores: opt: 416, E(): 1.2e-19, (35.7% identity in 255 aa overlap). Middle of the putative protein highly similar to N-terminal end of Q49860|B229_C2_182 hypothetical 11.0 KDA protein from Mycobacterium leprae (95 aa), FASTA scores: opt: 304, E(): 7.9e-13, (83.65% identity in 55 aa overlap). Also some similarity with other bacterial proteins e.g. P95886 ORF C02006 from Sulfolobus solfataricus (269 aa), FASTA scores: opt: 293, E(): 9.6e-12, (31.3% identity in 198 aa overlap); Q9XDF3|NONC NONC protein from Streptomyces griseus subsp. griseus (317 aa), FASTA scores: opt: 270, E(): 3.4e-10, (29.95% identity in 227 aa overlap); Q54229|NONR macrotetrolide antibiotic-resistance protein from Streptomyces griseus (347 aa), FASTA scores: opt: 270, E(): 3.6e-10, (29.95% identity in 227 aa overlap); etc.; hypothetical protein 3939257 888298 Rv3510c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218027.1 3938421 R 83332 CDS YP_177980.1 57117116 888273 3939617..3941761 1 NC_000962.3 Rv3511, (MTV023.18), len: 714 aa. PE_PGRS55, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002),similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK47974|MT3615.3 (1217 aa) FASTA scores: opt: 2563, E(): 1.5e-94, (59.65% identity in 773 aa overlap); and upstream O53553|Rv3508|MTV023.15 (1901 aa),FASTA scores: opt: 2455, E(): 3.9e-90, (60.4% identity in 737 aa overlap); etc. Contains PS00583 pfkB family of carbohydrate kinases signature 1.; PE-PGRS family protein PE_PGRS55 3941761 PE_PGRS55 888273 PE_PGRS55 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS55 YP_177980.1 3939617 D 83332 CDS YP_177981.1 57117117 888306 <3941724..3944963 1 NC_000962.3 Rv3512, (MTV023.19), len: 1079 aa. PE_PGRS56, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK47974|MT3615.3 (1217 aa) FASTA scores: opt: 3688, E(): 4.5e-130, (53.95% identity in 1136 aa overlap); and downstream O53559|Rv3514|MTV023.21 (1489 aa), FASTA scores: opt: 3611, E(): 3.6e-127, (53.15% identity in 1195 aa overlap); etc. Frameshifted PGRS protein, could be continuation of upstream MTV023.18, but no error could be found.; PE-PGRS family protein PE_PGRS56 3944963 PE_PGRS56 888306 PE_PGRS56 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS56 YP_177981.1 <3941724 D 83332 CDS NP_218030.1 15610649 888277 complement(3945092..3945748) 1 NC_000962.3 Rv3513c, (MTV023.20c), len: 218 aa (Start uncertain). Probable fadD18, fatty-acid-CoA synthetase (C-terminal fragment), almost identical to C-terminal end of downstream O53560|FADD19|Rv3515c|MTV023.22c, probably result of partial gene duplication. Also similar at the C-terminus to other fatty-acid-CoA synthetases e.g. Q9EXL2|FADD from Streptomyces griseus (540 aa), FASTA scores: opt: 586, E(): 1.2e-28, (52.45% identity in 185 aa overlap); AAB87139|MIG medium chain acyl-CoA synthetase precursor from Mycobacterium avium (550 aa), FASTA scores: opt: 506, E(): 9.5e-24, (50.0% identity in 150 aa overlap); Q9A7C3|CC1801 putative 4-coumarate--CoA ligase from Caulobacter crescentus (561 aa), FASTA scores: opt: 430,E(): 4.4e-19, (45.75% identity in 153 aa overlap); Q9KDT0|BH1131 acid-CoA ligase from Bacillus halodurans (546 aa), FASTA scores: opt: 338, E(): 1.9e-13, (38.05% identity in 142 aa overlap); Q9RTR4|DR1692 long-chain fatty acid--CoA ligase from Deinococcus radiodurans (584 aa),FASTA scores: opt: 331, E(): 5.3e-13, (35.15% identity in 145 aa overlap); etc.; Probable fatty-acid-CoA ligase FadD18 (fragment) (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 3945748 fadD18 888277 fadD18 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD18 (fragment) (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_218030.1 3945092 R 83332 CDS YP_177982.1 57117118 888294 3945794..3950263 1 NC_000962.3 Rv3514, (MTV023.21), len: 1489 aa. PE_PGRS57, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK47971 (1715 aa) FASTA scores: opt: 6940,E(): 0, (67.0% identity in 1713 aa overlap); and upstream O53553|YZ08_MYCTU|Rv3508|MTV023.15 (1901 aa), FASTA scores: opt: 6598,E(): 0, (71.05% identity in 1533 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures 1.; PE-PGRS family protein PE_PGRS57 3950263 PE_PGRS57 888294 PE_PGRS57 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS57 YP_177982.1 3945794 D 83332 CDS YP_177983.1 57117119 888275 complement(3950824..3952470) 1 NC_000962.3 Rv3515c, (MTV023.22c), len: 548 aa. fadD19,fatty-acid-CoA synthetase, similar (or with similarity) to many e.g. Q9EXL2|FADD FADD protein from Streptomyces griseus (540 aa), FASTA scores: opt: 1449, E(): 1.5e-81,(46.0% identity in 535 aa overlap); AAB87139|MIG medium chain acyl-CoA synthetase precursor from Mycobacterium avium (550 aa), FASTA scores: opt: 1226, E(): 7.6e-68,(40.7% identity in 543 aa overlap); Q9A7C3|CC1801 putative 4-coumarate--CoA ligase from Caulobacter crescentus (561 aa), FASTA scores: opt: 979, E(): 1.2e-52, (34.05% identity in 531 aa overlap); O28502|AF1772 long-chain-fatty-acid--CoA ligase (FADD-7) from Archaeoglobus fulgidus (569 aa), FASTA scores: opt: 560,E(): 6.9e-27, (29.3% identity in 543 aa overlap); Q9A8N2|CC1321 long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (583 aa), FASTA scores: opt: 544,E(): 6.7e-26, (27.2% identity in 518 aa overlap); P29212|LCFA_ECOLI|FADD|OLDD|B1805 long-chain-fatty-acid--CoA ligase from Escherichia coli strain K12 (561 aa), FASTA scores: opt: 460, E(): 4e-22,(26.3% identity in 567 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Note that upstream MTV023.20c|Rv3513c|fadD18 is identical to C-terminal part of FADD19|Rv3515c|MTV023.22c (probably result of partial gene duplication).; Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 3952470 fadD19 888275 fadD19 Mycobacterium tuberculosis H37Rv Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) YP_177983.1 3950824 R 83332 CDS NP_218033.1 15610652 888301 3952544..3953335 1 NC_000962.3 Rv3516, (MTV023.23), len: 263 aa. Possible echA19,enoyl-CoA hydratase, similar to other e.g. Q9ZHG2|ECHA1 from Rhodococcus fascians (275 aa) FASTA scores: opt: 613,E(): 6.4e-32, (45.15% identity in 259 aa overlap); P76082|PAAF_ECOLI|B1393 from Escherichia coli strain K12 (255 aa), FASTA scores: opt: 523, E(): 3.3e-26, (33.6% identity in 256 aa overlap); Q9I393|PA1629 from Pseudomonas aeruginosa (261 aa), FASTA scores: opt: 475, E(): 3.8e-23,(36.85% identity in 247 aa overlap); etc. Also similar to many carnitine racemases eg BAB52369|MLL6015 from Rhizobium loti (Mesorhizobium loti) (257 aa), FASTA scores: opt: 546,E(): 1.1e-27, (36.65% identity in 251 aa overlap). Similar to several putative enoyl-CoA hydratases from Mycobacterium tuberculosis, e.g. P96404|ECHA1|Rv0222|MTCY08D5.17 (262 aa), FASTA scores: opt: 630, E(): 5.1e-33, (44.5% identity in 254 aa overlap); and O53783|ECHA5|Rv0675|MTV040.03 (263 aa) FASTA scores: opt: 499, E(): 1.1e-24, (40.5% identity in 252 aa overlap). Could belong to the enoyl-CoA hydratase/isomerase family.; Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 3953335 echA19 888301 echA19 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_218033.1 3952544 D 83332 CDS NP_218034.1 15610653 888284 3953431..3954270 1 NC_000962.3 Rv3517, (MTV023.24), len: 279 aa. Hypothetical protein, similar to several hypothetical mycobacterial proteins e.g. P71763|Rv1482c|MTCY277.03c from Mycobacterium tuberculosis strain H37Rv (339 aa) (alias AAK45794|MT1529 from Mycobacterium tuberculosis strain CDC1551 (292 aa) but longer) FASTA scores: opt: 1040, E(): 3.7e-60, (59.0% identity in 273 aa overlap); O07396|MAV346 from Mycobacterium avium (346 aa) FASTA scores: opt: 1018, E(): 1e-58, (57.2% identity in 278 aa overlap); O53421|Rv1073|MTV017.26 from Mycobacterium tuberculosis strain H37Rv (283 aa), FASTA scores: opt: 903, E(): 2.4e-51, (48.0% identity in 277 aa overlap); Q50134|U650AG|MLCB57.67c from Mycobacterium leprae (75 aa) FASTA scores: opt: 158, E(): 0.0015, (41.8% identity in 55 aa overlap); etc.; hypothetical protein 3954270 888284 Rv3517 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218034.1 3953431 D 83332 CDS NP_218035.1 15610654 888282 complement(3954325..3955521) 1 NC_000962.3 Rv3518c, (MTV023.25c), len: 398 aa. Probable cyp142,cytochrome P450 monoxygenase, member of Cytochrome P450 family and similar to many e.g. Q9L465|CYP162A1|NIKQ from Streptomyces tendae (396 aa) FASTA scores: opt: 798, E(): 2e-43, (36.7% identity in 403 aa overlap); P33271|CPXK_SACER|CYP107B1 from Saccharopolyspora erythraea (Streptomyces erythraeus) (405 aa), FASTA scores: opt: 725,E(): 9.1e-39, (37.1% identity in 407 aa overlap); Q9X8Q3|CYP107P1|SCH10.14c from Streptomyces coelicolor (411 aa), FASTA scores: opt: 691, E(): 1.3e-36, (37.2% identity in 317 aa overlap); etc. Also similar to Q50696|C124_MYCTU|CYP124|Rv2266|MT2328|MTCY339.44c from Mycobacterium tuberculosis strain H37Rv (428 aa) FASTA scores: opt: 692, E(): 1.2e-36, (36.8% identity in 402 aa overlap). Equivalent to AAK47979 from Mycobacterium tuberculosis strain CDC1551 (372 aa) but longer 26 aa. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family.; Probable cytochrome P450 monooxygenase 142 Cyp142 3955521 cyp142 888282 cyp142 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 monooxygenase 142 Cyp142 NP_218035.1 3954325 R 83332 CDS NP_218036.1 15610655 887247 3955550..3956260 1 NC_000962.3 Rv3519, (MTV023.26), len: 236 aa (start uncertain). Unknown protein. The C-terminal end is highly similar to N-terminal end of AAK47980|MT3620 hypothetical 7.8 KDA protein from Mycobacterium tuberculosis strain CDC1551 (73 aa), FASTA scores: opt: 279, E(): 9.4e-12, (95.65% identity in 46 aa overlap).; hypothetical protein 3956260 887247 Rv3519 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218036.1 3955550 D 83332 CDS NP_218037.1 15610656 887310 complement(3956325..3957368) 1 NC_000962.3 Rv3520c, (MTV023.27c), len: 347 aa. Possible coenzyme F420-dependent oxidoreductase, equivalent to Q9CCV8|ML0348 possible coenzyme F420-dependent oxidoreductase from Mycobacterium leprae (350 aa), FASTA scores: opt: 2029, E(): 9.1e-120, (86.85% identity in 342 aa overlap). Similar to many coenzyme F420-dependent enzymes (and other proteins) e.g. Q9AD98|SCI52.11c putative ATP/GTP-binding protein from Streptomyces coelicolor (351 aa), FASTA scores: opt: 859, E(): 1.6e-46, (41.9% identity in 346 aa overlap); Q9X7Y1|SC6A5.35 putative oxidoreductase from Streptomyces coelicolor (341 aa), FASTA scores: opt: 800, E(): 7.9e-43, (38.95% identity in 339 aa overlap); Q9ZA30|GRA-ORF29 putative FMN-dependent monooxygenase from Streptomyces violaceoruber (343 aa), FASTA scores: opt: 354, E(): 6.7e-15, (34.2% identity in 336 aa overlap); Q49598|mer coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase from Methanopyrus kandleri (349 aa), FASTA scores: opt: 283,E(): 1.9e-10, (26.75% identity in 329 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 227,E(): 5.8e-07, (26.35% identity in 334 aa overlap); O27784|MTH1752 coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (321 aa), FASTA scores: opt: 207, E(): 1e-05, (27.4% identity in 336 aa overlap); etc. Also similar to Q11030|YD60_MYCTU|Rv1360|MT1405|MTCY02B10.24 hypothetical 37.3 KDA protein from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 313, E(): 2.5e-12, (28.0% identity in 311 aa overlap).; Possible coenzyme F420-dependent oxidoreductase 3957368 887310 Rv3520c Mycobacterium tuberculosis H37Rv Possible coenzyme F420-dependent oxidoreductase NP_218037.1 3956325 R 83332 CDS NP_218038.1 15610657 887209 3957521..3958432 1 NC_000962.3 Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, E(): 6.8e-06, (27.45% identity in 153 aa overlap); BAB60424|TVG1322512 from Thermoplasma volcanium (164 aa), FASTA scores: opt: 183,E(): 0.00015, (29.75% identity in 148 aa overlap); etc. Equivalent to AAK47982 from Mycobacterium tuberculosis strain CDC1551 (334 aa) but shorter 31 aa.; hypothetical protein 3958432 887209 Rv3521 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218038.1 3957521 D 83332 CDS NP_218039.1 15610658 887885 3958448..3959512 1 NC_000962.3 Rv3522, (MTV023.29), len: 354 aa. Possible ltp4,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30103|AF0134 3-ketoacyl-CoA thiolase (ACAB-4) from Archaeoglobus fulgidus (398 aa) FASTA scores: opt: 352, E(): 5.3e-15, (30.45% identity in 381 aa overlap); O29295|AF0967 3-ketoacyl-CoA thiolase (ACAB-9) from Archaeoglobus fulgidus (400 aa) FASTA scores: opt: 312,E(): 1.8e-12, (28.05% identity in 367 aa overlap); O29294|AF0968 3-ketoacyl-CoA thiolase (ACAB-10) from Archaeoglobus fulgidus (388 aa), FASTA scores: opt: 293,E(): 2.9e-11, (25.9% identity in 309 aa overlap); O58409|PH0676 long hypothetical nonspecific lipid-transfer protein (acethyl CoA synthetase) from Pyrococcus horikoshii (389 aa), FASTA scores: opt: 292, E(): 3.3e-11, (25.8% identity in 368 aa overlap); Q9Y9A3|APE2382 long hypothetical non specific lipid-transfer protein from Aeropyrum pernix (360 aa) FASTA scores: opt: 270, E(): 7.8e-10, (27.25% identity in 363 aa overlap); Q9YDI4|APE0929 long hypothetical nonspecific lipid-transfer protein from Aeropyrum pernix (400 aa), FASTA scores: opt: 258, E(): 4.9e-09, (26.45% identity in 306 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Possible lipid transfer protein or keto acyl-CoA thiolase Ltp4 3959512 ltp4 887885 ltp4 Mycobacterium tuberculosis H37Rv Possible lipid transfer protein or keto acyl-CoA thiolase Ltp4 NP_218039.1 3958448 D 83332 CDS NP_218040.1 15610659 888247 3959529..3960713 1 NC_000962.3 Rv3523, (MTCY03C7.33c), len: 394 aa. Probable ltp3,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30037|AF0202 3-ketoacyl-CoA thiolase (ACAB-6) from Archaeoglobus fulgidus (380 aa) FASTA scores: opt: 782, E(): 1.7e-40, (38.35% identity in 386 aa overlap); Q9Y9A1|APE2384 long hypothetical non specific lipid-transfer protein (acethyl CoA synthetase) from Aeropyrum pernix (394 aa), FASTA scores: opt: 626, E(): 5.9e-31, (35.75% identity in 386 aa overlap); BAB59210|TVG0067506 lipid transfer protein from Thermoplasma volcanium (390 aa), FASTA scores: opt: 591,E(): 8.1e-29, (34.35% identity in 384 aa overlap); Q9YDI4|APE0929 long hypothetical nonspecific lipid-transfer protein from Aeropyrum pernix (400 aa) FASTA scores: opt: 588, E(): 1.3e-28, (31.6% identity in 408 aa overlap); O30104|AF0133 3-ketoacyl-CoA thiolase (ACAB-3) from Archaeoglobus fulgidus (411 aa) FASTA scores: opt: 583,E(): 2.6e-28, (39.8% identity in 412 aa overlap); O29811|AF0438 3-ketoacyl-CoA thiolase (ACAB-8) from Archaeoglobus fulgidus (387 aa), FASTA scores: opt: 574,E(): 8.8e-28, (30.95% identity in 388 aa overlap); etc.; Probable lipid carrier protein or keto acyl-CoA thiolase Ltp3 3960713 ltp3 888247 ltp3 Mycobacterium tuberculosis H37Rv Probable lipid carrier protein or keto acyl-CoA thiolase Ltp3 NP_218040.1 3959529 D 83332 CDS NP_218041.1 15610660 888250 3960755..3961786 1 NC_000962.3 Rv3524, (MTCY03C7.32c), len: 343 aa. Probable conserved membrane protein, showing some similarity to C-terminal part of putative Mycobacterium tuberculosis proteins O05871|P95308|PKND_MYCTU|Rv0931c|MT0958|MTCY08C9.08 serine-threonine protein kinase PknD (664 aa) FASTA scores: opt: 727, E(): 8.3e-36, (45.3% identity in 298 aa overlap); O53893|Rv0980c|MTV044.08c PGRS-family protein (457 aa),FASTA scores: opt: 208, E(): 4.4e-05, (33.75% identity in 166 aa overlap); and O53891|Rv0978c|MTV044.06c PGRS-family protein (331 aa) FASTA scores: opt: 153, E(): 0.062,(30.75% identity in 117 aa overlap). Contains PS00237 G-protein coupled receptors signature.; Probable conserved membrane protein 3961786 888250 Rv3524 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_218041.1 3960755 D 83332 CDS NP_218042.1 15610661 888255 complement(3961800..3962324) 1 NC_000962.3 Rv3525c, (MTCY3C7.31), len: 174 aa. Possible siderophore-binding protein, similar to ferripyochelin binding proteins (and related) e.g. Q9RSN5|DR2089 ferripyochelin-binding protein from Deinococcus radiodurans (240 aa), FASTA scores: opt: 472, E(): 3.3e-21, (46.9% identity in 162 aa overlap); O59257|PH1591 long hypothetical ferripyochelin binding protein from Pyrococcus horikoshii (173 aa), FASTA scores: opt: 431, E(): 6.7e-19,(40.0% identity in 170 aa overlap); Q9V158|FBP|PAB0393 ferripyochelin binding protein from Pyrococcus abyssi (173 aa), FASTA scores: opt: 429, E(): 8.9e-19, (39.4% identity in 170 aa overlap); BAB47820|MLR0180 ferripyochelin binding protein-like from Rhizobium loti (Mesorhizobium loti) (175 aa), FASTA scores: opt: 415, E(): 6.1e-18, (42.55% identity in 141 aa overlap); etc.; Possible siderophore-binding protein 3962324 888255 Rv3525c Mycobacterium tuberculosis H37Rv Possible siderophore-binding protein NP_218042.1 3961800 R 83332 CDS NP_218043.1 15610662 888268 3962439..3963599 1 NC_000962.3 Rv3526, (MTCY03C7.30c), len: 386 aa. kshA, oxygenase component of 3-ketosteroid-9-alpha-hydroxylase, highly similar, except in C-terminus (also longer 69 aa), to O69348|ORF12 protein (function unknown) from Rhodococcus erythropolis (316 aa) FASTA scores: opt: 1137, E(): 6.9e-65, (59.6% identity in 250 aa overlap). Also some similarity with several aminopyrrolnitrin oxidases (PRND proteins, involved in the pathway for pyrrolnitrin biosynthesis, a secondary metabolite derived from tryptophan which has strong anti-fungal activity) e.g. Q9RPG0|PRND from Myxococcus fulvus (379 aa), FASTA scores: opt: 322, E(): 4.4e-13, (25.85% identity in 352 aa overlap); Q9RPG4|PRND from Burkholderia cepacia (Pseudomonas cepacia) (373 aa) FASTA scores: opt: 306, E(): 4.5e-12, (25.2% identity in 373 aa overlap); P95483|PRND from Pseudomonas fluorescens (363 aa), FASTA scores: opt: 305, E(): 5.1e-12, (25.0% identity in 372 aa overlap); etc. And also some similarity to other putative enzymes like dioxygenases, oxidases, vanillate O-demethyl oxygenase,etc.; Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA 3963599 kshA 888268 kshA Mycobacterium tuberculosis H37Rv Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA NP_218043.1 3962439 D 83332 CDS NP_218044.1 15610663 888295 3963605..3964054 1 NC_000962.3 Rv3527, (MTCY03C7.29c), len: 149 aa. Hypothetical unknown protein.; Hypothetical protein 3964054 888295 Rv3527 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218044.1 3963605 D 83332 CDS NP_218045.1 15610664 888297 complement(3964479..3965192) 1 NC_000962.3 Rv3528c, (MTCY03C7.28), len: 237 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 3965192 888297 Rv3528c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218045.1 3964479 R 83332 CDS NP_218046.1 15610665 888324 complement(3965884..3967038) 1 NC_000962.3 Rv3529c, (MTCY03C7.27), len: 384 aa. Conserved hypothetical protein, showing some similarity to Q50695|YM67_MYCTU|Rv2267c|MT2329|MTCY339.43 hypothetical 46.1 KDA protein from Mycobacterium tuberculosis (388 aa) FASTA scores: opt: 261, E(): 1.6e-09, (27.25% identity in 253 aa overlap).; hypothetical protein 3967038 888324 Rv3529c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218046.1 3965884 R 83332 CDS NP_218047.1 15610666 888338 complement(3967038..3967820) 1 NC_000962.3 Rv3530c, (MTCY03C7.26), len: 260 aa. Possible oxidoreductase, similar to various oxidoreductases and hypothetical proteins e.g. BAB53258|Q987E5|MLL7083 probable oxidoreductase from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 405, E(): 5.3e-18, (33.45% identity in 263 aa overlap); Q9VNF3|CG12171 hypothetical protein from Drosophila melanogaster (Fruit fly) (257 aa),FASTA scores: opt: 404, E(): 6.1e-18, (32.8% identity in 256 aa overlap); Q9A3X5|CC3076 oxidoreductase (short-chain dehydrogenase/reductase family) from Caulobacter crescentus (254 aa), FASTA scores: opt: 400, E(): 1.1e-17, (31.0% identity in 255 aa overlap); BAB50080|MLR3115 dehydrogenase from Rhizobium loti (Mesorhizobium loti) (259 aa), FASTA scores: opt: 393, E(): 3e-17, (31.9% identity in 254 aa overlap); Q9F5J1|SIM-NJ1|SIMD2 putative 3-keto-acyl-reductase from Streptomyces antibioticus (273 aa), FASTA scores: opt: 388, E(): 6.3e-17, (31.6% identity in 250 aa overlap); etc.; Possible oxidoreductase 3967820 888338 Rv3530c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_218047.1 3967038 R 83332 CDS NP_218048.1 15610667 888348 complement(3967817..3968944) 1 NC_000962.3 Rv3531c, (MTCY03C7.25), len: 375 aa. Hypothetical unknown protein.; Hypothetical protein 3968944 888348 Rv3531c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218048.1 3967817 R 83332 CDS YP_177984.1 57117120 888370 3969343..3970563 1 NC_000962.3 Rv3532, (MTCY03C7.24c), len: 406 aa. PPE61, Member of the Mycobacterium tuberculosis PPE protein family,similar to many, e.g. O53956|Rv1807|MTV049.29 (403 aa),FASTA scores: opt: 954, E(): 1.1e-43, (44.1% identity in 417 aa overlap); PPE family protein PPE61 3970563 PPE61 888370 PPE61 Mycobacterium tuberculosis H37Rv PPE family protein PPE61 YP_177984.1 3969343 D 83332 CDS YP_177985.1 57117121 888385 complement(3970705..3972453) 1 NC_000962.3 Rv3533c, (MTCY03C7.23), len: 582 aa. PPE62, Member of the Mycobacterium tuberculosis PPE protein family,similar to many, e.g. O53309|Rv3159c|MTV014.03c (590 aa) FASTA scores: opt: 2289, E(): 2.3e-95, (63.5% identity in 600 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; PPE family protein PPE62 3972453 PPE62 888385 PPE62 Mycobacterium tuberculosis H37Rv PPE family protein PPE62 YP_177985.1 3970705 R 83332 CDS NP_218051.1 15610670 888387 complement(3972552..3973592) 1 NC_000962.3 Rv3534c, (MTCY03C7.22), len: 346 aa. Probable hsaF,4-hydroxy-2-oxovalerate aldolase, highly similar to others e.g. P51015|BPHI_PSESP from Pseudomonas sp. strain LB400 (346 aa), FASTA scores: opt: 1150, E(): 2.3e-61, (51.35% identity in 331 aa overlap); Q52040|BPHX3 from Pseudomonas pseudoalcaligenes (346 aa), FASTA scores: opt: 1147, E(): 3.5e-61, (51.35% identity in 331 aa overlap); P51017|NAHM_PSEPU from Pseudomonas putida (346 aa), FASTA scores: opt: 1145, E(): 4.7e-61, (50.9% identity in 330 aa overlap) (see citation below); P51020|MHPE_ECOLI|MHPF|B0352 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 1133, E(): 2.4e-60, (52.0% identity in 327 aa overlap); O24833|ATDG from Acinetobacter sp (340 aa), FASTA scores: opt: 1132, E(): 2.7e-60, (50.45% identity in 331 aa overlap); etc. Note that also highly similar to Q9ZI56|NAHM 2-oxo-4-hydroxypentanoate aldolase from Pseudomonas stutzeri (Pseudomonas perfectomarina) (346 aa) FASTA scores: opt: 1168, E(): 2e-62, (51.05% identity in 331 aa overlap) (see citation below).; Probable 4-hydroxy-2-oxovalerate aldolase (HOA) 3973592 hsaF 888387 hsaF Mycobacterium tuberculosis H37Rv Probable 4-hydroxy-2-oxovalerate aldolase (HOA) NP_218051.1 3972552 R 83332 CDS NP_218052.1 15610671 888396 complement(3973589..3974500) 1 NC_000962.3 Rv3535c, (MTCY03C7.21), len: 303 aa. Probable hsaG,acetaldehyde dehydrogenase, highly similar to many e.g. BAB62056|TDNI from Pseudomonas putida (302 aa), FASTA scores: opt: 1159, E(): 1.5e-62, (60.45% identity in 301 aa overlap); Q9ZI57|NAHO from Pseudomonas stutzeri (Pseudomonas perfectomarina) (307 aa) FASTA scores: opt: 1151, E(): 4.6e-62, (59.55% identity in 299 aa overlap); Q9F9I4|CDOI from Comamonas sp. JS765 (302 aa) FASTA scores: opt: 1136, E(): 3.6e-61, (60.15% identity in 301 aa overlap); Q51962|NAHO from Pseudomonas putida (307 aa),FASTA scores: opt: 1133, E(): 5.6e-61, (58.55% identity in 299 aa overlap) (see citation below); P77580|MHPF_ECOLI|MHPF|MHPE|B0351 from Escherichia coli strain K12 (316 aa), FASTA scores: opt: 1040, E(): 2.2e-55,(56.85% identity in 306 aa overlap); etc.; Probable acetaldehyde dehydrogenase (acetaldehyde dehydrogenase [acetylating]) 3974500 hsaG 888396 hsaG Mycobacterium tuberculosis H37Rv Probable acetaldehyde dehydrogenase (acetaldehyde dehydrogenase [acetylating]) NP_218052.1 3973589 R 83332 CDS NP_218053.1 15610672 888406 complement(3974511..3975296) 1 NC_000962.3 Rv3536c, (MTCY03C7.20), len: 261 aa. Probable hsaE,hydratase, 2-oxo-hepta-3-ene-1,7-dioate hydratase or 2-keto-4-pentenoate hydratase. Indeed, highly similar to many 2-oxo-hepta-3-ene-1,7-dioate hydratases e.g. Q9CKS2|HPAH|PM1534 from Pasteurella multocida (267 aa) FASTA scores: opt: 743, E(): 1.5e-39, (45.5% identity in 266 aa overlap) Q9RZ31|DRA0122 from Deinococcus radiodurans (268 aa), FASTA scores: opt: 709, E(): 2e-37, (45.5% identity in 266 aa overlap); Q9HWQ4|HPCG|PA4127 from Pseudomonas aeruginosa (267 aa), FASTA scores: opt: 703,E(): 4.8e-37, (45.1% identity in 266 aa overlap); Q46982|HPAH|HPCG from Escherichia colis strain ATCC 11105 (267 aa), FASTA scores: opt: 679, E(): 1.6e-35, (41.35% identity in 266 aa overlap); etc. But also highly similar to many 2-keto-4-pentenoate hydratases (2-hydroxypentadienoic acidhydratases) e.g. Q9LAF7|PHED from Bacillus thermoglucosidasius (258 aa), FASTA scores: opt: 698, E(): 9.7e-37, (42.45% identity in 252 aa overlap); Q52442|BPHH from Pseudomonas sp (260 aa) FASTA scores: opt: 675, E(): 2.7e-35, (41.4% identity in 251 aa overlap); P77608|MHPD_ECOLI|B0350 from Escherichia coli strain K12 (269 aa), FASTA scores: opt: 674, E(): 3.2e-35,(42.75% identity in 255 aa overlap); Q52038|BPHX1 from Pseudomonas pseudoalcaligenes (260 aa), FASTA scores: opt: 663, E(): 1.5e-34, (40.6% identity in 251 aa overlap); etc.; Probable hydratase 3975296 hsaE 888406 hsaE Mycobacterium tuberculosis H37Rv Probable hydratase NP_218053.1 3974511 R 83332 CDS NP_218054.1 15610673 888422 3975369..3977060 1 NC_000962.3 Rv3537, (MTCY03C7.19c), len: 563 aa. Probable kstD,dehydrogenase, similar to many dehydrogenases or hypothetical proteins e.g. Q9I1M6|PA2243 hypothetical protein from Pseudomonas aeruginosa (577 aa), FASTA scores: opt: 984, E(): 1.2e-48, (34.75% identity in 573 aa overlap); Q06401|3O1D_COMTE 3-oxosteroid 1-dehydrogenase from Comamonas testosteroni (Pseudomonas testosteroni) (573 aa), FASTA scores: opt: 955, E(): 5.5e-47, (33.05% identity in 590 aa overlap); Q9RA02|KSTD1 3-ketosteroid dehydrogenase from Rhodococcus erythropolis (510 aa), FASTA scores: opt: 631, E(): 1.4e-28, (39.15% identity in 557 aa overlap); P77815|KSDD 3-ketosteroid-1-dehydrogenase from Nocardioides simplex (Arthrobacter simplex) (515 aa), FASTA scores: opt: 469, E(): 2.4e-19, (35.45% identity in 564 aa overlap); etc.; Probable dehydrogenase 3977060 kstD 888422 kstD Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_218054.1 3975369 D 83332 CDS YP_177986.1 57117122 888426 3977062..3977922 1 NC_000962.3 Rv3538, (MTCY03C7.18c), len: 286 aa. Probable double hotdog R-specific hydratase, substrate unknown, shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below) especially Rv3389. Probable dehydrogenase, similar to Q9L009|SCC30.12c putative dehydrogenase from Streptomyces coelicolor (333 aa), FASTA scores: opt: 842, E(): 3.6e-44, (48.4% identity in 285 aa overlap); and similar to C-terminal part of other (principally estradiol 17 beta-dehydrogenases/17-beta-hydroxysteroid dehydrogenases) e.g. P70540 peroxisomal multifunctional enzyme type II (SDR family) from Rattus norvegicus (Rat) (735 aa) FASTA scores: opt: 622, E(): 1.9e-30, (37.45% identity in 283 aa overlap); or P70523|MPF-2 multifunctional protein 2 (SDR family) (beta-oxidation protein displaying 2-enoyl-CoA hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity) from Rattus norvegicus (Rat) (734 aa), FASTA scores: opt: 616, E(): 4.3e-30, (37.1% identity in 283 aa overlap); P51659|DHB4_HUMAN|HSD17B4|EDH17B4 estradiol 17 beta-dehydrogenase from Homo sapiens (Human) (736 aa),FASTA scores: opt: 614, E(): 5.7e-30, (35.9% identity in 284 aa overlap); P97852|DHB4_RAT|HSD17B4|EDH17B4 estradiol 17 beta-dehydrogenase from Rattus norvegicus (Rat) (735 aa) FASTA scores: opt: 613, E(): 6.6e-30, (37.1% identity in 283 aa overlap); Q9DBM3|HSD17B4 estradiol 17 beta-dehydrogenase from Mus musculus (Mouse) (735 aa) FASTA scores: opt: 611, E(): 8.7e-30, (36.5% identity in 285 aa overlap); etc. Also similar to Q11198|Rv3389c|MTV004.47c hypothetical 30.3 KDA protein from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 609, E(): 5.3e-30, (39.65% identity in 285 aa overlap). Note that previously known as ufaA2.; Probable dehydrogenase Possible 2-enoyl acyl-CoA hydratase 3977922 888426 Rv3538 Mycobacterium tuberculosis H37Rv Probable dehydrogenase Possible 2-enoyl acyl-CoA hydratase YP_177986.1 3977062 D 83332 CDS YP_177987.1 57117123 888438 3978059..3979498 1 NC_000962.3 Rv3539, (MTCY03C7.17c), len: 479 aa. PPE63, Member of the Mycobacterium tuberculosis PPE protein family,similar to many e.g. O53949|Rv1800|MTV049.22 (655 aa),FASTA scores: opt: 914, E(): 7.3e-47, (37.55% identity in 490 aa overlap); etc.; PPE family protein PPE63 3979498 PPE63 888438 PPE63 Mycobacterium tuberculosis H37Rv PPE family protein PPE63 YP_177987.1 3978059 D 83332 CDS NP_218057.1 15610676 888450 complement(3979499..3980659) 1 NC_000962.3 Rv3540c, (MTCY03C7.16), len: 386 aa. Probable ltp2,lipid-transfer protein or keto acyl-CoA thiolase, similar to several e.g. Q9X4X2|DITF DITF protein (hypothetical protein, similar to non-specific lipid-transfer protein and 3-ketoacyl-CoA thiolase) from Pseudomonas abietaniphila (397 aa), FASTA scores: opt: 665, E(): 5.3e-34, (33.4% identity in 392 aa overlap); O30255|AF2416 3-ketoacyl-CoA thiolase (ACAB-12) from Archaeoglobus fulgidus (384 aa),FASTA scores: opt: 496, E(): 1.6e-23, (30.35% identity in 389 aa overlap); O28978|AF1291 3-ketoacyl-CoA thiolase (ACAB-11) from Archaeoglobus fulgidus (392 aa), FASTA scores: opt: 494, E(): 2.2e-23, (30.6% identity in 379 aa overlap); O26884|MTH793 lipid-transfer protein (sterol or nonspecific) from Methanobacterium thermoautotrophicum (383 aa), FASTA scores: opt: 487, E(): 5.9e-23, (30.4% identity in 388 aa overlap); etc.; Probable lipid transfer protein or keto acyl-CoA thiolase Ltp2 3980659 ltp2 888450 ltp2 Mycobacterium tuberculosis H37Rv Probable lipid transfer protein or keto acyl-CoA thiolase Ltp2 NP_218057.1 3979499 R 83332 CDS NP_218058.1 15610677 888475 complement(3980659..3981048) 1 NC_000962.3 Rv3541c, (MTCY03C7.15), len: 129 aa. Conserved protein, showing some similarity to Q9CBJ7|ML1909 hypothetical protein from Mycobacterium leprae (142 aa) FASTA scores: opt: 110, E(): 1.2, (27.95% identity in 118 aa overlap); and other (see also blastp results) e.g. Q9L0M3|SCD82.08 hypothetical 15.2 KDA protein from Streptomyces coelicolor (142 aa), FASTA scores: opt: 127,E(): 0.086, (27.65% identity in 123 aa overlap). Contains PS00075 Dihydrofolate reductase signature.; hypothetical protein 3981048 888475 Rv3541c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218058.1 3980659 R 83332 CDS NP_218059.1 15610678 888486 complement(3981045..3981980) 1 NC_000962.3 Rv3542c, (MTCY03C7.14), len: 311 aa. Hypothetical protein, showing some similarity to other e.g. Q58947|MJ1552 from Methanococcus jannaschii (141 aa) FASTA scores: opt: 177, E(): 0.00065, (46.65% identity in 60 aa overlap); BAB59276|TVG0142586 from Thermoplasma volcanium (135 aa), FASTA scores: opt: 175, E(): 0.00083, (35.65% identity in 87 aa overlap); Q9HI85|TA1457 from Thermoplasma acidophilum (135 aa), FASTA scores: opt: 162, E(): 0.0052,(31.8% identity in 107 aa overlap); etc.; hypothetical protein 3981980 888486 Rv3542c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218059.1 3981045 R 83332 CDS NP_218060.1 15610679 888131 complement(3981977..3983140) 1 NC_000962.3 Rv3543c, (MTCY03C7.13), len: 387 aa. Probable fadE29, acyl-CoA dehydrogenase, similar to many e.g. Q9A8P3|CC1310 from Caulobacter crescentus (404 aa), FASTA scores: opt: 624, E(): 9.4e-32, (32.75% identity in 400 aa overlap); Q9I4V2|PA1022 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 550, E(): 3.9e-27, (33.7% identity in 350 aa overlap); O28976|AF1293 from Archaeoglobus fulgidus (384 aa), FASTA scores: opt: 529, E(): 8.1e-26,(30.0% identity in 393 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. O53549|FADE26|Rv3504|MTV023.11 (400 aa), FASTA scores: opt: 1031, E(): 2.8e-57, (46.0% identity in 402 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE29 3983140 fadE29 888131 fadE29 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE29 NP_218060.1 3981977 R 83332 CDS NP_218061.1 15610680 888091 complement(3983125..3984144) 1 NC_000962.3 Rv3544c, (MTCY03C7.12), len: 339 aa. Probable fadE28, acyl-CoA dehydrogenase, similar to many e.g. Q9RJX3|SCF37.28c from Streptomyces coelicolor (362 aa),FASTA scores: opt: 334, E(): 5.1e-13, (27.65% identity in 329 aa overlap); Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 278, E(): 1.2e-09, (26.95% identity in 319 aa overlap); O29813|AF0436 from Archaeoglobus fulgidus (382 aa) FASTA scores: opt: 205,E(): 3.5e-05, (24.75% identity in 384 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. O53550|FADE27|Rv3505|MTV023.12 (373 aa) FASTA scores: opt: 497, E(): 7e-23, (30.3% identity in 343 aa overlap); and to P46703|ACDP_MYCLE|FADE25|ACD|ML0737|B1308_F1_34 probable acyl-CoA dehydrogenase from Mycobacterium leprae (389 aa) FASTA scores: opt: 165, E(): 0.0012, (25.2% identity in 345 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE28 3984144 fadE28 888091 fadE28 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE28 NP_218061.1 3983125 R 83332 CDS NP_218062.1 15610681 887782 complement(3984144..3985445) 1 NC_000962.3 Rv3545c, (MT3649, MTCY03C7.11), len: 433 aa. Probable cyp125, cytochrome P-450, similar to others e.g. Q59723|LINC|CYP111 from Pseudomonas incognita (406 aa),FASTA scores: opt: 831, E(): 8e-45, (34.75% identity in 406 aa overlap); Q9X8Q3|CYP107P1|SCH10.14c from Streptomyces coelicolor (411 aa), FASTA scores: opt: 694, E(): 3.3e-36,(32.35% identity in 417 aa overlap); Q9L465|CYP162A1|NIKQ from Streptomyces tendae (396 aa) FASTA scores: opt: 664,E(): 2.5e-34, (34.15% identity in 413 aa overlap); O08469|CPXY_BACSU|CYPA|CYP107J1 from Bacillus subtilis (410 aa), FASTA scores: opt: 579, E(): 5.6e-29, (30.05% identity in 366 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. Q50696|CYP124|Rv2266|MT2328|MTCY339.44c (428 aa) FASTA scores: opt: 1040, E(): 6.1e-58, (40.75% identity in 432 aa overlap). Belongs to the cytochrome P450 family.; Probable cytochrome P450 125 Cyp125 3985445 cyp125 887782 cyp125 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 125 Cyp125 NP_218062.1 3984144 R 83332 CDS NP_218063.1 15610682 887360 3985557..3986732 1 NC_000962.3 Rv3546, (MTCY03C7.10c), len: 391 aa. Probable fadA5,acetyl-CoA acetyltransferase, similar to many e.g. Q9AA29|CC0779 from Caulobacter crescentus (390 aa), FASTA scores: opt: 999, E(): 7.1e-54, (43.5% identity in 400 aa overlap); Q9K783|BH3487 from Bacillus halodurans (393 aa),FASTA scores: opt: 843, E(): 2.6e-44, (37.45% identity in 398 aa overlap); Q9RRK9|DR2480 from Deinococcus radiodurans (399 aa), FASTA scores: opt: 826, E(): 2.8e-43, (38.15% identity in 396 aa overlap); P45369|THIL_CHRVI|PHBA from Chromatium vinosum (394 aa) FASTA scores: opt: 790, E(): 4.5e-41, (39.4% identity in 401 aa overlap); etc. Contains PS00737 Thiolases signature 2. Belongs to the thiolase family.; Probable acetyl-CoA acetyltransferase FadA5 (acetoacetyl-CoA thiolase) 3986732 fadA5 887360 fadA5 Mycobacterium tuberculosis H37Rv Probable acetyl-CoA acetyltransferase FadA5 (acetoacetyl-CoA thiolase) NP_218063.1 3985557 D 83332 CDS NP_218064.1 15610683 887496 3986844..3987299 1 NC_000962.3 Rv3547, (MTCY03C7.09c), len: 151 aa. Ddn,deazaflavin-dependent nitroreducatse (See Singh et al.,2008). Similar to hypothetical proteins e.g. O85698|3SCF60.07 from Streptomyces lividans and Streptomyces coelicolor (149 aa), FASTA scores: opt: 353,E(): 6.3e-17, (42.55% identity in 134 aa overlap); Q9WX21|SCE68.11 from Streptomyces coelicolor (305 aa) FASTA scores: opt: 290, E(): 2.1e-12, (38.5% identity in 122 aa overlap) (similarity in N-terminus for this protein); BAB52932|Q988L5|MLL6688 from Rhizobium loti (Mesorhizobium loti) (148 aa), FASTA scores: opt: 105, E(): 3, (26.75% identity in 86 aa overlap). Also similar to mycobacterial hypothetical proteins e.g. Q9ZH81 from Mycobacterium paratuberculosis (144 aa), FASTA scores: opt: 366, E(): 8.2e-18, (43.9% identity in 123 aa overlap); and Q10772|YF58_MYCTU|Rv1558|MT1609|MTCY48.07c from Mycobacterium tuberculosis (148 aa), FASTA scores: opt: 330, E(): 2.2e-15, (39.75% identity in 151 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008).; Deazaflavin-dependent nitroreductase Ddn 3987299 ddn 887496 ddn Mycobacterium tuberculosis H37Rv Deazaflavin-dependent nitroreductase Ddn NP_218064.1 3986844 D 83332 CDS NP_218065.1 15610684 887452 complement(3987382..3988296) 1 NC_000962.3 Rv3548c, (MTCY03C7.08), len: 304 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases/reductases (generally belonging to the SDR family) e.g. Q9I4V1|PA1023 from Pseudomonas aeruginosa (305 aa), FASTA scores: opt: 446, E(): 1.7e-17,(43.75% identity in 256 aa overlap); Q9A6K0|CC2093 from Caulobacter crescentus (301 aa) FASTA scores: opt: 437,E(): 5.3e-17, (42.8% identity in 257 aa overlap); Q9HYH8|PA3427 from Pseudomonas aeruginosa (303 aa), FASTA scores: opt: 399, E(): 6.5e-15, (45.5% identity in 257 aa overlap); Q9VXJ0|CG3415 from Drosophila melanogaster (Fruit fly) (598 aa), FASTA scores: opt: 402, E(): 7.5e-15, (40.7% identity in 285 aa overlap); etc. Also highly similar to O53547|Rv3502c|MTV023.09c putative short-chain type dehydrogenase/reductase from (317 aa) FASTA scores: opt: 739, E(): 1.6e-33, (45.15% identity in 310 aa overlap); and other proteins from Mycobacterium tuberculosis. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 3988296 887452 Rv3548c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_218065.1 3987382 R 83332 CDS NP_218066.1 15610685 887849 complement(3988319..3989098) 1 NC_000962.3 Rv3549c, (MTCY03C7.07), len: 259 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases (generally belong to the SDR family) e.g. Q9UKU3 from Homo sapiens (Human) (270 aa),FASTA scores: opt: 451, E(): 4.8e-21, (38.05% identity in 247 aa overlap); Q9S274|SCI28.09c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 439, E(): 2.4e-20,(36.8% identity in 231 aa overlap); Q9PFI6|XF0671 from Xylella fastidiosa (247 aa), FASTA scores: opt: 437, E(): 3.4e-20, (37.7% identity in 252 aa overlap); etc. Also highly similar to O33308|FABG5|Rv2766c|MTV002.31c alcohol dehydrogenase (SDR family) from Mycobacterium tuberculosis (260 aa), FASTA scores: opt: 504, E(): 2.3e-24, (38.5% identity in 244 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Probable short-chain type dehydrogenase/reductase 3989098 887849 Rv3549c Mycobacterium tuberculosis H37Rv Probable short-chain type dehydrogenase/reductase NP_218066.1 3988319 R 83332 CDS NP_218067.1 15610686 888232 3989153..3989896 1 NC_000962.3 Rv3550, (MTCY03C7.06c), len: 247 aa. Probable echA20, enoyl-CoA hydratase, similar to others e.g. Q9A7B0|CC1814 from Caulobacter crescentus (275 aa), FASTA scores: opt: 488, E(): 3.5e-24, (36.4% identity in 239 aa overlap); O84978|PHAA from Pseudomonas putida (293 aa),FASTA scores: opt: 383, E(): 2e-17, (33.85% identity in 254 aa overlap); BAB48479|Q98LI4|MLL1009 from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 378, E(): 3.8e-17, (21.45% identity in 231 aa overlap); etc. Could belong to the enoyl-CoA hydratase/isomerase family.; Probable enoyl-CoA hydratase EchA20 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 3989896 echA20 888232 echA20 Mycobacterium tuberculosis H37Rv Probable enoyl-CoA hydratase EchA20 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_218067.1 3989153 D 83332 CDS NP_218068.1 15610687 887235 3989896..3990774 1 NC_000962.3 Rv3551, (MTCY03C7.05c), len: 292 aa. Possible CoA-transferase, alpha subunit, similar in part to other CoA-transferases e.g. Q59111|GCTA_ACIFE|GCTA glutaconate CoA-transferase subunit A (GCT large subunit) from Acidaminococcus fermentans (319 aa) FASTA scores: opt: 247,E(): 6.3e-09, (27.35% identity in 307 aa overlap); Q9XD83|PCAI from Streptomyces sp. 2065 (251 aa), FASTA scores: opt: 222, E(): 2.3e-07, (27.55% identity in 243 aa overlap); BAB50895|MLL4183 from Rhizobium loti (Mesorhizobium loti) (285 aa), FASTA scores: opt: 206, E(): 2.8e-06, (27.4% identity in 281 aa overlap); etc. Also some similarity with O06167|SCOA_MYCTU|RVv504c|MT2579|MTCY07A7.10c probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 210, E(): 1.4e-06, (25.5% identity in 247 aa overlap). Belongs to the glutaconate CoA-transferase subunit A family. Note that this putative protein may combine with the putative protein encoded by the downstream ORF Rv3552 to form a CoA-transferase that comprises two subunits.; Possible CoA-transferase (alpha subunit) 3990774 887235 Rv3551 Mycobacterium tuberculosis H37Rv Possible CoA-transferase (alpha subunit) NP_218068.1 3989896 D 83332 CDS NP_218069.1 15610688 887453 3990771..3991523 1 NC_000962.3 Rv3552, (MTCY03C7.03c), len: 250 aa. Possible CoA-transferase, beta subunit, similar in part to other CoA-transferases e.g. Q9I6R1|PA0227 from Pseudomonas aeruginosa (260 aa), FASTA scores: opt: 233, E(): 8.6e-08,(24.8% identity in 238 aa overlap); BAB50894|MLL4181 from Rhizobium loti (Mesorhizobium loti) (264 aa), FASTA scores: opt: 210, E(): 2.6e-06, (24.15% identity in 203 aa overlap); and AAK41345|Q97Z51|GCTB from Sulfolobus solfataricus (245 aa), FASTA scores: opt: 122, E(): 1.1,(25.5% identity in 243 aa overlap). Possibly belongs to the glutaconate CoA-transferase subunit B family. Note that this putative protein may combine with the putative protein encoded by the upstream ORF Rv3551 to form a CoA-transferase that comprises two subunits.; Possible CoA-transferase (beta subunit) 3991523 887453 Rv3552 Mycobacterium tuberculosis H37Rv Possible CoA-transferase (beta subunit) NP_218069.1 3990771 D 83332 CDS NP_218070.1 15610689 887190 3991621..3992688 1 NC_000962.3 Rv3553, (MTCY03C7.02c), len: 355 aa. Possible oxidoreductase, highly similar (except in C-terminus) to Q9A327|CC3379 hypothetical protein from Caulobacter crescentus (321 aa), FASTA scores: opt: 639, E(): 4.6e-29,(46.35% identity in 248 aa overlap); and Q9WZQ7|TM0800 conserved hypothetical protein from Thermotoga maritima (314 aa), FASTA scores: opt: 622, E(): 4.1e-28, (37.95% identity in 340 aa overlap). Also similar to two trans-2-enoyl-ACP reductases; Q99YD4|FABK|SPY1751 from Streptococcus pyogenes (323 aa), FASTA scores: opt: 604,E(): 4.4e-27, (33.25% identity in 346 aa overlap); and Q9FBC5|FABK from Streptococcus pneumoniae (324 aa), FASTA scores: opt: 553, E(): 3.3e-24, (32.1% identity in 346 aa overlap); and similar with several 2-nitropropane dioxygenases, e.g. Q9F7P8 from uncultured proteobacterium EBAC31A08 (322 aa), FASTA scores: opt: 505, E(): 1.7e-21,(33.6% identity in 348 aa overlap); Q9FMG0 (alias AAK44141) from Arabidopsis thaliana (Mouse-ear cress) (333 aa), FASTA scores: opt: 489, E(): 1.4e-20, (33.15% identity in 341 aa overlap); O28109|AF2173 (NCD2) from Archaeoglobus fulgidus (274 aa), FASTA scores: opt: 456, E(): 8.9e-19, (36.3% identity in 237 aa overlap); etc.; Possible oxidoreductase 3992688 887190 Rv3553 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_218070.1 3991621 D 83332 CDS NP_218071.1 15610690 887221 3992685..3994742 1 NC_000962.3 Rv3554, (MTCY06G11.01, MTCY03C7.01c), len: 685 aa. Possible fdxB, two-domain protein, with ferredoxin reductase electron transfer component in C-terminal part and unknown function in N-terminal part. Indeed, N-terminal end is similar to O85832 hypothetical 36.1 KDA protein from Sphingomonas aromaticivorans strain F199 (catabolic plasmid pNL1) (309 aa), FASTA scores: opt: 615, E(): 2.5e-30,(33.1% identity in 311 aa overlap); and P73428|SLL1468 hypothetical 36.2 KDA protein from Synechocystis sp. strain PCC 6803 (312 aa), FASTA scores: opt: 317, E(): 4.5e-12,(30.2% identity in 268 aa overlap). And C-terminal end is similar to Q9F9U6|PAAE protein involved in aerobic phenylacetate metabolism from Azoarcus evansii (360 aa),FASTA scores: opt: 935, E(): 7e-50, (43.85% identity in 351 aa overlap); CAC44653|PAAE|SCBAC17A6.08 putative phenylacetic acid degradation NADH oxidoreductase from Streptomyces coelicolor (368 aa), FASTA scores: opt: 93,E(): 9.5e-50, (41.95% identity in 372 aa overlap); Q9FA57|PACI ferredoxin from Azoarcus evansii (360 aa),FASTA scores: opt: 925, E(): 2.9e-49, (43.3% identity in 351 aa overlap); P76081|PAAE_ECOLI|B1392 probable phenylacetic acid degradation NADH oxidoreductase from Escherichia coli strains K12 and W (356 aa), FASTA scores: opt: 910, E(): 2.4e-48, (43.05% identity in 353 aa overlap); Q9APJ6|PAAE electron transfer protein (fragment) from Hyphomicrobium chloromethanicum (241 aa), FASTA scores: opt: 404, E(): 1.7e-17, (35.45% identity in 234 aa overlap); BAB51608|MLL5100 ferredoxin from Rhizobium loti (Mesorhizobium loti) (365 aa), FASTA scores: opt: 316, E(): 5.8e-12, (28.95% identity in 349 aa overlap); etc. C-terminus also similar to P96853|Rv3571|MTCY06G11.18 putative electron transfer protein from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 450, E(): 3.6e-20, (32.95% identity in 358 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Belongs to the 2FE2S plant-type ferredoxin family. Cofactor: binds a 2FE-2S cluster (by similarity).; Possible electron transfer protein FdxB 3994742 fdxB 887221 fdxB Mycobacterium tuberculosis H37Rv Possible electron transfer protein FdxB NP_218071.1 3992685 D 83332 CDS NP_218072.1 15610691 887952 complement(3994830..3995699) 1 NC_000962.3 Rv3555c, (MTCY06G11.02c), len: 289 aa. Conserved protein, highly similar to others from Mycobacterium tuberculosis e.g. O53562|AL022022|Rv3517|MTV023.24 (279 aa), FASTA scores: opt: 874, E(): 8.3e-48, (49.45% identity in 275 aa overlap); P71763|Rv1482c|MTCY277.03c (339 aa),FASTA scores: opt: 755, E(): 3e-40, (45.75% identity in 260 aa overlap); O69681|Rv3714c|MTV025.062c (296 aa), FASTA scores: opt: 733, E(): 6.4e-39, (44.1% identity in 281 aa overlap); etc. Also highly similar to other mycobacterial hypothetical proteins e.g. O07396|MAV346 from Mycobacterium avium (346 aa), FASTA scores: opt: 714, E(): 1.1e-37,(44.6% identity in 260 aa overlap); and Q50134|U650AG|MLCB57.67c from Mycobacterium leprae (75 aa),FASTA scores: opt: 130, E(): 0.17, (35.1% identity in 57 aa overlap) (only partial homology with this protein). Shows some similarity to P52392|NHSR_STRAS putative nosiheptide resistance regulatory protein (ORF699) from Streptomyces actuosus (233 aa), FASTA scores: opt: 120, E(): 1.9,(25.25% identity in 194 aa overlap).; hypothetical protein 3995699 887952 Rv3555c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218072.1 3994830 R 83332 CDS NP_218073.1 15610692 887285 complement(3995804..3996964) 1 NC_000962.3 Rv3556c, (MTCY06G11.03), len: 386 aa. Probable fadA6, acetyl-CoA acetyltransferase, similar to many e.g. Q9K409|2SCG61.06c from Streptomyces coelicolor (389 aa),FASTA scores: opt: 1091, E(): 2.9e-58, (48.1% identity in 399 aa overlap); Q9AAT4|CC0510 from Caulobacter crescentus (391 aa), FASTA scores: opt: 902, E(): 6.6e-47, (40.25% identity in 395 aa overlap); P45359|THL_CLOAB from Clostridium acetobutylicum (392 aa), FASTA scores: opt: 872, E(): 4.2e-45, (37.9% identity in 396 aa overlap); Q9I2A8|ATOB|PA2001 from Pseudomonas aeruginosa (393 aa),FASTA scores: opt: 872, E(): 4.2e-45, (41.3% identity in 397 aa overlap); etc. Contains PS00737 Thiolases signature 2. Belongs to the thiolase family.; Probable acetyl-CoA acetyltransferase FadA6 (acetoacetyl-CoA thiolase) 3996964 fadA6 887285 fadA6 Mycobacterium tuberculosis H37Rv Probable acetyl-CoA acetyltransferase FadA6 (acetoacetyl-CoA thiolase) NP_218073.1 3995804 R 83332 CDS NP_218074.1 15610693 887467 complement(3997029..3997631) 1 NC_000962.3 Rv3557c, (MTCY06G11.04c), len: 200 aa. Transcriptional regulator, TetR family, similar to other e.g. Q9RRV9|DR2376 from Deinococcus radiodurans (197 aa) FASTA scores: opt: 326, E(): 2.3e-14, (31.2% identity in 189 aa overlap); Q9HZW2|PA2885 from Pseudomonas aeruginosa (198 aa), FASTA scores: opt: 308, E(): 3.5e-13, (31.55% identity in 187 aa overlap); Q9RFR4 from Pseudomonas fluorescens (207 aa), FASTA scores: opt: 291, E(): 4.7e-12,(29.75% identity in 195 aa overlap); Q9K8P5|BH2958 from Bacillus halodurans (215 aa), FASTA scores: opt: 271, E(): 9.9e-11, (23.95% identity in 192 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. O53641|Rv0158|MTV032.01 (214 aa), FASTA scores: opt: 232,E(): 3.5e-08, (25.5% identity in 192 aa overlap); and O06169|Rv2506|MTCY07A7.12 (215 aa), FASTA scores: opt: 215,E(): 4.5e-07, (35.15% identity in 148 aa overlap); etc. Seems to belong to the TetR/AcrR family of transcriptional regulators.; Transcriptional regulatory protein (probably TetR-family) 3997631 887467 Rv3557c Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein (probably TetR-family) NP_218074.1 3997029 R 83332 CDS YP_177988.1 57117124 887822 3997980..3999638 1 NC_000962.3 Rv3558, (MTCY06G11.05), len: 552 aa. PPE64, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. P71868|Rv3533c|MTCY03C7.23 (582 aa), FASTA scores: opt: 1908, E(): 1.7e-83, (58.5% identity in 583 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).; PPE family protein PPE64 3999638 PPE64 887822 PPE64 Mycobacterium tuberculosis H37Rv PPE family protein PPE64 YP_177988.1 3997980 D 83332 CDS NP_218076.1 15610695 887897 complement(3999647..4000435) 1 NC_000962.3 Rv3559c, (MTCY06G11.06c), len: 262 aa. Probable oxidoreductase, similar to various oxidoreductases e.g. Q9F5J1|SIM-NJ1|SIMD2 putative 3-keto-acyl-reductase (SDR family) from Streptomyces antibioticus (273 aa), FASTA scores: opt: 510, E(): 2.8e-24, (40.15% identity in 249 aa overlap);Q9L2C9|SC7A8.29 putative dehydrogenase from Streptomyces coelicolor (255 aa), FASTA scores: opt: 500,E(): 1.1e-23, (41.4% identity in 239 aa overlap); Q9HQ41|FABG|VNG1341G 3-oxoacyl-[acyl-carrier-protein] reductase from Halobacterium sp. strain NRC-1 (255 aa) FASTA scores: opt: 500, E(): 1.1e-23, (40.0% identity in 250 aa overlap); etc. Also similar to oxidoreductases from Mycobacterium tuberculosis eg Q11020|YD50_MYCTU|FABG2|Rv1350|MT1393|MTCY02B10.14 putative oxidoreductase (247 aa), FASTA scores: opt: 497, E(): 1.6e-23, (39.2% identity in 245 aa overlap).; Probable oxidoreductase 4000435 887897 Rv3559c Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_218076.1 3999647 R 83332 CDS NP_218077.1 15610696 887838 complement(4000432..4001589) 1 NC_000962.3 Rv3560c, (MTCY06G11.07c), len: 385 aa. Probable fadE30, acyl-CoA dehydrogenase, similar to many e.g. Q9I4V2|PA1022 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 845, E(): 1.6e-47, (39.2% identity in 388 aa overlap); Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 734, E(): 2.8e-40, (35.5% identity in 386 aa overlap); Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa), FASTA scores: opt: 656, E(): 3.2e-35,(37.9% identity in 351 aa overlap); etc. Also similar to acyl-CoA dehydrogenases from Mycobacterium tuberculosis e.g. P95280|FADE17|Rv1934c|MTCY09F9.30 (409 aa), FASTA scores: opt: 939, E(): 1.4e-53, (43.8% identity in 404 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE30 4001589 fadE30 887838 fadE30 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE30 NP_218077.1 4000432 R 83332 CDS NP_218078.1 15610697 887244 4001637..4003160 1 NC_000962.3 Rv3561, (MTCY06G11.08), len: 507 aa. Probable fadD3,fatty-acid-CoA synthetase, similar to many substrate-CoA symthetases/ligases e.g. Q9KBC2|BH2006 long-chain acyl-CoA synthetase from Bacillus halodurans (513 aa), FASTA scores: opt: 821, E(): 1.6e-43, (32.9% identity in 517 aa overlap); Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. HR167 (491 aa) FASTA scores: opt: 767, E(): 3.5e-40,(37.65% identity in 502 aa overlap); Q9ZIP5|MATB malonyl CoA synthetase from Rhizobium leguminosarum (504 aa), FASTA scores: opt: 758, E(): 1.3e-39, (33.7% identity in 472 aa overlap); Q9CD27|FADD2|ML2546 acyl-CoA synthase from Mycobacterium leprae (548 aa), FASTA scores: opt: 700, E(): 5.6e-36, (31.85% identity in 515 aa overlap); P29212|LCFA_ECOLI|FADD|OLDD|B1805 long-chain-fatty-acid--CoA ligase from Escherichia coli strain K12 (561 aa), FASTA scores: opt: 532, E(): 6.3e-28,(30.0% identity in 533 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis eg O53306|FADD13|Rv3089|MTV013.10 (503 aa), FASTA scores: opt: 819, E(): 2.1e-43, (35.1% identity in 490 aa overlap). Contains PS00455 Putative AMP-binding domain signature.; Probable fatty-acid-CoA ligase FadD3 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) 4003160 fadD3 887244 fadD3 Mycobacterium tuberculosis H37Rv Probable fatty-acid-CoA ligase FadD3 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) NP_218078.1 4001637 D 83332 CDS NP_218079.1 15610698 887884 4003161..4004294 1 NC_000962.3 Rv3562, (MTCY06G11.09), len: 377 aa. Probable fadE31, acyl-CoA dehydrogenase, similar to many e.g. Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa),FASTA scores: opt: 657, E(): 1.7e-34, (36.45% identity in 351 aa overlap); Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 653, E(): 3.2e-34, (33.95% identity in 392 aa overlap); Q9EX72|MLHC from Rhodococcus erythropolis (324 aa) FASTA scores: opt: 631, E(): 6.5e-33,(36.95% identity in 330 aa overlap); P45867|ACDA_BACSU|ACD from Bacillus subtilis (379 aa), FASTA scores: opt: 347,E(): 1e-15, (28.6% identity in 385 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. P96842|FADE30|Rv3560c|MTCY06G11.07c (385 aa), FASTA scores: opt: 843, E(): 2.3e-46, (38.95% identity in 380 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE31 4004294 fadE31 887884 fadE31 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE31 NP_218079.1 4003161 D 83332 CDS NP_218080.1 15610699 887818 4004291..4005250 1 NC_000962.3 Rv3563, (MTCY06G11.10), len: 319 aa. Probable fadE32, acyl-CoA dehydrogenase, similar to many e.g. Q9I4V4|PA1020 from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 347, E(): 7.6e-14, (35.15% identity in 333 aa overlap); Q9RJX3|SCF37.28c from Streptomyces coelicolor (362 aa), FASTA scores: opt: 300, E(): 5.3e-11, (32.4% identity in 349 aa overlap); Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 285, E(): 4.1e-10,(30.4% identity in 329 aa overlap); P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 230,E(): 1.1e-07, (25.5% identity in 357 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis eg P96846|FADE33|Rv3564|MTCY06G11.11 (318 aa), FASTA scores: opt: 478, E(): 7.6e-22, (32.9% identity in 292 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE32 4005250 fadE32 887818 fadE32 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE32 NP_218080.1 4004291 D 83332 CDS NP_218081.1 15610700 888458 4005247..4006203 1 NC_000962.3 Rv3564, (MTCY06G11.11), len: 318 aa. Probable fadE33, acyl-CoA dehydrogenase, similar to others e.g. Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 373, E(): 1.9e-15, (34.3% identity in 338 aa overlap); Q9I4V4|PA1020 from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 277, E(): 1.4e-09, (31.95% identity in 335 aa overlap); Q9X7Y6|SC6A5.40c from Streptomyces coelicolor (395 aa), FASTA scores: opt: 273, E(): 2.5e-09,(30.1% identity in 352 aa overlap); P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 478,E(): 7.9e-22, (32.9% identity in 292 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. P96845|FADE32|Rv3563|MTCY06G11.10 (319 aa), FASTA scores: opt: 478, E(): 7.9e-22, (32.9% identity in 292 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE33 4006203 fadE33 888458 fadE33 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE33 NP_218081.1 4005247 D 83332 CDS NP_218082.1 15610701 888305 4006200..4007366 1 NC_000962.3 Rv3565, (MTCY06G11.12), len: 388 aa. Possible aspB,aspartate aminotransferase, similar to many e.g. Q9A5J2|CC2455 aminotransferase class I from Caulobacter crescentus (381 aa), FASTA scores: opt: 1112, E(): 1e-61,(45.85% identity in 384 aa overlap); Q9HV76|PA4722 probable aminotransferase from Pseudomonas aeruginosa (390 aa),FASTA scores: opt: 863, E(): 3.1e-46, (37.2% identity in 390 aa overlap); Q9RWP3|DR0623 aspartate aminotransferase from Deinococcus radiodurans (388 aa), FASTA scores: opt: 713, E(): 6.3e-37, (35.5% identity in 383 aa overlap); Q9HQK2|ASPC2|VNG1121G aspartate aminotransferase from Halobacterium sp. strain NRC-1 (391 aa), FASTA scores: opt: 710, E(): 9.8e-37, (34.45% identity in 380 aa overlap); O33822|AAT_THEAQ|ASPC aspartate aminotransferase from Thermus aquaticus (383 aa), FASTA scores: opt: 695, E(): 8.2e-36, (35.1% identity in 376 aa overlap); etc. Contains PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site. Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate (by similarity).; Possible aspartate aminotransferase AspB (transaminase A) (ASPAT) (glutamic--oxaloacetic transaminase) (glutamic--aspartic transaminase) 4007366 aspB 888305 aspB Mycobacterium tuberculosis H37Rv Possible aspartate aminotransferase AspB (transaminase A) (ASPAT) (glutamic--oxaloacetic transaminase) (glutamic--aspartic transaminase) NP_218082.1 4006200 D 83332 CDS YP_177989.1 57117125 888005 complement(4007331..4008182) 1 NC_000962.3 Rv3566c, (MT3671, MTCY06G11.13c), len: 283 aa. Nat (alternate gene name: nhoA), arylamine N-acetyltransferase (see citations below), highly similar to O86309|NAT_MYCSM arylamine N-acetyltransferase from Mycobacterium smegmatis (see citation below) (275 aa), FASTA scores: opt: 1114,E(): 3e-66, (60.95% identity in 274 aa overlap). Also highly similar to others e.g. Q98D42|BAB51429|MLR4870 from Rhizobium loti (Mesorhizobium loti) (278 aa), FASTA scores: opt: 697, E(): 1.1e-38, (44.1% identity in 272 aa overlap); P77567|NHOA_ECOLI|B1463 from Escherichia coli strain K12 (281 aa), FASTA scores: opt: 537, E(): 4.4e-28, (38.85% identity in 273 aa overlap); Q00267|NHOA_SALTY from Salmonella typhimurium (281 aa), FASTA scores: opt: 507,E(): 4.3e-26, (34.8% identity in 273 aa overlap); etc. Belongs to the arylamine N-acetyltransferase family. Note that previously known as nhoA (332 aa) and that nucleotide 4007874 has been changed since first submission (G deleted).; Arylamine N-acetyltransferase Nat (arylamine acetylase) 4008182 nat 888005 nat Mycobacterium tuberculosis H37Rv Arylamine N-acetyltransferase Nat (arylamine acetylase) YP_177989.1 4007331 R 83332 CDS YP_177990.1 57117126 3205089 complement(4008167..4008433) 1 NC_000962.3 Rv3566A, len: 88 aa. Hypothetical unknown protein.; Hypothetical protein 4008433 3205089 Rv3566A Mycobacterium tuberculosis H37Rv Hypothetical protein YP_177990.1 4008167 R 83332 CDS NP_218084.1 15610703 887525 complement(4008719..4009282) 1 NC_000962.3 Rv3567c, (MTCY06G11.14c), len: 187 aa. Possible hsaB, oxidoreductase, similar to various oxidoreductases and hypothetical proteins e.g. O69360 ORF61 protein from Rhodococcus erythropolis (194 aa) FASTA scores: opt: 974,E(): 3e-59, (77.05% identity in 183 aa overlap); Q9JN75|MMYF putative oxidoreductase from Streptomyces coelicolor (174 aa), FASTA scores: opt: 451, E(): 1e-23,(43.65% identity in 158 aa overlap); P54990|NTAB_CHEHE|NMOB nitrilotriacetate monooxygenase component B from Chelatobacter heintzii (322 aa), FASTA scores: opt: 409,E(): 1.3e-20, (38.3% identity in 167 aa overlap)Chelatobacter heintzii; AAK62356 putative NADH:FMN oxidoreductase from Burkholderia sp. DBT1 (177 aa), FASTA scores: opt: 360, E(): 1.6e-17, (36.15% identity in 155 aa overlap).; Possible oxidoreductase Possible 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione hydroxylase 4009282 hsaB 887525 hsaB Mycobacterium tuberculosis H37Rv Possible oxidoreductase Possible 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione hydroxylase NP_218084.1 4008719 R 83332 CDS NP_218085.1 15610704 887886 complement(4009297..4010199) 1 NC_000962.3 Rv3568c, (MTCY06G11.15c), len: 300 aa. HsaC, highly similar to e.g. Q9KWQ5|BPHC5 from Rhodococcus sp. RHA1 (300 aa), FASTA scores: opt: 1715, E(): 3.8e-103, (82.15% identity in 297 aa overlap); O50479|EDOB from Rhodococcus rhodochrous (300 aa) FASTA scores: opt: 1714, E(): 4.4e-103, (82.5% identity in 297 aa overlap); O69359|BPHC6 from Rhodococcus erythropolis (300 aa), FASTA scores: opt: 1647, E(): 9.1e-99, (78.25% identity in 299 aa overlap); Q9RBT2|BPHC1 from Pseudomonas sp. SY5 (301 aa) Pseudomonas sp. SY5 (298 aa) FASTA scores: opt: 767, E(): 3.9e-42,(42.8% identity in 299 aa overlap); P47228|BPHC_BURCE from Burkholderia cepacia (Pseudomonas cepacia) (297 aa), FASTA scores: opt: 670, E(): 6.8e-36, (40.55% identity in 296 aa overlap); etc. Contains PS00082 Extradiol ring-cleavage dioxygenases signature. Belongs to the extradiol ring-cleavage dioxygenase family.; 3,4-DHSA dioxygenase 4010199 hsaC 887886 hsaC Mycobacterium tuberculosis H37Rv 3,4-DHSA dioxygenase NP_218085.1 4009297 R 83332 CDS NP_218086.1 15610705 887378 complement(4010196..4011071) 1 NC_000962.3 Rv3569c, (MTCY06G11.16c), len: 291 aa. HsaD, highly similar to e.g. Q9KWQ6|BPHD2 from Rhodococcus sp. RHA1 (292 aa), FASTA scores: opt: 1468, E(): 1.3e-85, (75.5% identity in 294 aa overlap); Q52036 from Pseudomonas putida (286 aa), FASTA scores: opt: 785, E(): 1.9e-42, (45.1% identity in 295 aa overlap); Q52011|BPHD from Pseudomonas pseudoalcaligenes (286 aa), FASTA scores: opt: 774, E(): 9.3e-42, (44.05% identity in 295 aa overlap); P47229|BPHD_BURCE from Burkholderia cepacia (Pseudomonas cepacia) (286 aa) FASTA scores: opt: 772, E(): 1.2e-41,(44.5% identity in 295 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A. Similar to alpha/beta hydrolase fold.; 4,9-DHSA hydrolase 4011071 hsaD 887378 hsaD Mycobacterium tuberculosis H37Rv 4,9-DHSA hydrolase NP_218086.1 4010196 R 83332 CDS NP_218087.1 15610706 887241 complement(4011086..4012270) 1 NC_000962.3 Rv3570c, (MTCY06G11.17c), len: 394 aa. Possible hsaA, oxidoreductase, most similar to hydroxylases and oxygenases (and also some similarity to acyl-CoA dehydrogenases) e.g. O69349 hydroxylase from Rhodococcus erythropolis (393 aa), FASTA scores: opt: 958, E(): 1.1e-53, (39.95% identity in 383 aa overlap); P26698|PIGM_RHOSO pigment protein from Rhodococcus sp. strain ATCC 21145 (387 aa), FASTA scores: opt: 665, E(): 5.4e-35, (32.2% identity in 382 aa overlap); Q9ZGA9|LANZ5 oxygenase homolog from Streptomyces cyanogenus (397 aa) FASTA scores: opt: 588, E(): 4.5e-30, (30.55% identity in 386 aa overlap); Q9F0J3|NCNH hydroxylase from Streptomyces arenae (405 aa), FASTA scores: opt: 580, E(): 1.5e-29,(31.25% identity in 336 aa overlap); O69789|BPFA indole dioxygenase from Rhodococcus opacus (399 aa), FASTA scores: opt: 558, E(): 3.7e-28, (31.8% identity in 387 aa overlap); etc.; Possible oxidoreductase Possible 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione hydroxylase 4012270 hsaA 887241 hsaA Mycobacterium tuberculosis H37Rv Possible oxidoreductase Possible 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione hydroxylase NP_218087.1 4011086 R 83332 CDS NP_218088.1 15610707 887315 4012417..4013493 1 NC_000962.3 Rv3571, (MTCY06G11.18), len: 358 aa. kshB, reductase component of 3-ketosteroid-9-alpha-hydroxylase, similar to several e.g. Q44253|ATDA5 aniline dioxygenase reductase component from Acinetobacter sp (336 aa) FASTA scores: opt: 748, E(): 1.5e-38, (34.95% identity in 346 aa overlap); P95533|TDNB electron transfer protein from Pseudomonas putida (337 aa), FASTA scores: opt: 723, E(): 5.2e-37,(36.35% identity in 341 aa overlap); AAK65059|SMA0752 possible dioxygenase reductase subunit from Rhizobium meliloti (Sinorhizobium meliloti) (353 aa) FASTA scores: opt: 495, E(): 4.9e-23, (31.9% identity in 345 aa overlap); P76081|PAAE_ECOLI|B1392 probable phenylacetic acid degradation NADH oxidoreductase (356 aa), FASTA scores: opt: 364, E(): 5.1e-15, (34.45% identity in 357 aa overlap); Q9L131|HMPA flavohemoprotein from Streptomyces coelicolor (398 aa), FASTA scores: opt: 352, E(): 3e-14,(32.8% identity in 247 aa overlap); etc. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that it has been shown hmp transcription increased at early stationary phase and is lower at late stationary phase and during exponential growth. Note that previously known as hmp.; Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB 4013493 kshB 887315 kshB Mycobacterium tuberculosis H37Rv Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB NP_218088.1 4012417 D 83332 CDS NP_218089.1 15610708 887227 4013511..4014041 1 NC_000962.3 Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 4014041 887227 Rv3572 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218089.1 4013511 D 83332 CDS NP_218090.1 15610709 887843 complement(4014077..4016212) 1 NC_000962.3 Rv3573c, (MTCY06G11.20c), len: 711 aa. Probable fadE34, acyl-CoA dehydrogenase, similar to others,especially in C-terminal half, e.g. Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa) FASTA scores: opt: 780,E(): 2.8e-39, (44.1% identity in 347 aa overlap); Q9A6N8|CC2049 from Caulobacter crescentus (401 aa), FASTA scores: opt: 705, E(): 8.7e-35, (41.5% identity in 342 aa overlap); Q9EX72|MLHC from Rhodococcus erythropolis (324 aa), FASTA scores: opt: 673, E(): 6.1e-33, (42.05% identity in 283 aa overlap); P41367|ACDM_PIG|ACADM from Sus scrofa (Pig)(421 aa) FASTA scores: opt: 325, E(): 4.9e- 13, (28.5% identity in 368 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. P95097|FADE22|Rv3061c|MTCY22D7.20 (721 aa), FASTA scores: opt: 1635, E(): 2.7e-90, (42.65% identity in 729 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE34 4016212 fadE34 887843 fadE34 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE34 NP_218090.1 4014077 R 83332 CDS NP_218091.3 448824816 887204 4016484..4017083 1 NC_000962.3 Rv3574, (MTCY06G11.21), len: 199 aa. Probable kstR,transcriptional regulator TetR family, similar to others e.g. Q9KXK1|SCC53.10 from Streptomyces coelicolor (250 aa) FASTA scores: opt: 492, E(): 4.8e-25, (44.8% identity in 183 aa overlap); Q9RA03|KSTR from Rhodococcus erythropolis (208 aa), FASTA scores: opt: 294, E(): 3.1e-12, (28.9% identity in 187 aa overlap); BAB54261|MLR7895 from Rhizobium loti (Mesorhizobium loti) (193 aa), FASTA scores: opt: 166, E(): 0.00062, (32.05% identity in 78 aa overlap); P17446|BETI_ECOLI|B0313 from Escherichia coli strain K12 (195 aa), FASTA scores: opt: 142, E(): 0.0034, (25. 6% identity in 168 aa overlap); etc. Equivalent to AAK48038 from Mycobacterium tuberculosis strain CDC1551 (243 aa) but shorter 44 aa. Contains possible helix-turn-helix motif from aa 37-58 (+3.70 SD). Possibly belongs to the TetR/AcrR family of transcriptional regulators.; Transcriptional regulatory protein KstR (probably TetR-family) 4017083 kstR 887204 kstR Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein KstR (probably TetR-family) NP_218091.3 4016484 D 83332 CDS NP_218092.1 15610711 888084 complement(4017089..4018168) 1 NC_000962.3 Rv3575c, (MTCY06G11.22c), len: 359 aa. Probable transcriptional regulator belonging to lacI family, similar to others e.g. BAB53947|MLL8376 from Rhizobium loti (Mesorhizobium loti) (358 aa), FASTA scores: opt: 707, E(): 2.6e-35, (35.5% identity in 355 aa overlap); Q9RRI9|DR2501 from Deinococcus radiodurans (359 aa) FASTA scores: opt: 544, E(): 1.6e-25, (40.35% identity in 347 aa overlap); Q9RL31|SCF51A.34 from Streptomyces coelicolor (347 aa),FASTA scores: opt: 307, E(): 2.9e-11, (30.0% identity in 330 aa overlap); O87590|CELR_THEFU from Thermomonospora fusca (340 aa), FASTA scores: opt: 280, E(): 1.2e-09,(32.3% identity in 353 aa overlap); P21867|RAFR_ECOLI from Escherichia coli (335 aa) FASTA scores: opt: 241, E(): 2.6e-07, (27.15% identity in 269 aa overlap); etc. Equivalent to AAK48039 from Mycobacterium tuberculosis strain CDC1551 (404 aa) but shorter 45 aa. Contains possible helix-turn-helix motif, at aa 9-30 (+5.86 SD). Could belong to the LacI family of transcriptional regulators.; Transcriptional regulatory protein (probably LacI-family) 4018168 888084 Rv3575c Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein (probably LacI-family) NP_218092.1 4017089 R 83332 CDS YP_177991.1 57117127 888444 4018358..4019071 1 NC_000962.3 Rv3576, (MTCY06G11.23), len: 237 aa. Possible lppH,conserved lipoprotein, similar in part with proteins from Mycobacterium tuberculosis; C-terminus of Q11053|PKNH_MYCTU|PKNH|Rv1266c|MT1304|MTCY50.16 probable serine/threonine-protein kinase (626 aa) FASTA scores: opt: 396, E(): 6.5e-19, (36.0% identity in 200 aa overlap); and with P71740|LPPR|Rv2403c|MTCY253.17 probable lipoprotein protein (251 aa), FASTA scores: opt: 134, E(): 0.087,(22.7% identity in 207 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as pknM.; Possible conserved lipoprotein LppH 4019071 lppH 888444 lppH Mycobacterium tuberculosis H37Rv Possible conserved lipoprotein LppH YP_177991.1 4018358 D 83332 CDS NP_218094.1 15610713 888442 4019262..4020128 1 NC_000962.3 Rv3577, (MTCY06G11.24), len: 288 aa (other start sites possible upstream; equivalent to AAK48041 from Mycobacterium tuberculosis strain CDC1551 (379 aa) but shorter 91 aa). Hypothetical protein, showing some similarity to Q9RI88|SCJ11.16c hypothetical 37.9 KDA protein from Streptomyces coelicolor (349 aa) FASTA scores: opt: 285, E(): 1.5e-10, (27.45% identity in 266 aa overlap).; hypothetical protein 4020128 888442 Rv3577 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218094.1 4019262 D 83332 CDS NP_218095.1 15610714 888329 4020142..4021383 1 NC_000962.3 Rv3578, (MTCY06G11.25), len: 413 aa. Possible arsB2,arsenical pump integral membrane protein, similar to many e.g. Q9I1J6|ARSB|PA2278 from Pseudomonas aeruginosa (427 aa), FASTA scores: opt: 375, E(): 3.1e-15, (32.15% identity in 429 aa overlap); Q9K8K7|ARSB|BH2999 from Bacillus halodurans (436 aa), FASTA scores: opt: 360, E(): 2.5e-14,(28.7% identity in 432 aa overlap); P52146|ARB2_ECOLI from Escherichia coli (plasmid R46) (429 aa), FASTA scores: opt: 345, E(): 2e-13, (29.8% identity in 426 aa overlap); etc. Also highly similar to Q9KYM0|SC9H11.21c probable membrane efflux protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 730, E(): 1.7e-36, (53.95% identity in 443 aa overlap). Seems to belong to the ARS family.; Possible arsenical pump integral membrane protein ArsB2 4021383 arsB2 888329 arsB2 Mycobacterium tuberculosis H37Rv Possible arsenical pump integral membrane protein ArsB2 NP_218095.1 4020142 D 83332 CDS NP_218096.1 15610715 888317 complement(4021425..4022393) 1 NC_000962.3 Rv3579c, (MTCY06G11.26c), len: 322 aa. Possible tRNA/rRNA methyltransferase, equivalent, but longer 31 aa,to Q9CCW4|ML0324 putative methyltransferase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1517,E(): 3.4e-79, (83.75% identity in 277 aa overlap). Also highly similar to Q9L0Q5|SCD8A.09 from Streptomyces coelicolor (314 aa), FASTA scores: opt: 937, E(): 3.4e-46,(56.75% identity in 319 aa overlap); and similar to others e.g. Q06753|YACO_BACSU from Bacillus subtilis (249 aa),FASTA scores: opt: 616, E(): 4.9e-28, (41.05% identity in 246 aa overlap); Q9KGF2|BH0113 from Bacillus halodurans (249 aa), FASTA scores: opt: 596, E(): 6.7e-27, (38.5% identity in 244 aa overlap); P74328|Y955_SYNY3|SLR0955 from Synechocystis sp. strain PCC 6803 (384 aa), FASTA scores: opt: 585, E(): 4e-26, (35.85% identity in 304 aa overlap); P39290|YJFH_ECOLI|B4180 from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 521, E(): 1.2e-22, (38.1% identity in 244 aa overlap); etc. Equivalent to AAK48043 from Mycobacterium tuberculosis strain CDC1551 (253 aa) but longer 69 aa. Possibly belongs to the RNA methyltransferase TrmH family.; Possible tRNA/rRNA methyltransferase 4022393 888317 Rv3579c Mycobacterium tuberculosis H37Rv Possible tRNA/rRNA methyltransferase NP_218096.1 4021425 R 83332 CDS YP_177992.1 57117128 888628 complement(4022394..4023803) 1 NC_000962.3 Rv3580c, (MTCY06G11.27c), len: 469 aa. Probable cysS1, cysteinyl-tRNA synthetase, equivalent to P57990|SYC1_MYCLE|CYSS1|CYSS|ML0323 cysteinyl-tRNA synthetase 1 from Mycobacterium leprae (473 aa) FASTA scores: opt: 2825, E(): 3.4e-172, (86.5% identity in 467 aa overlap). Also similar to many e.g. Q9L0Q6|SCD8A.08 from Streptomyces coelicolor (613 aa), FASTA scores: opt: 1834,E(): 4.7e-109, (57.5% identity in 461 aa overlap); Q9I2U7|CYSS|PA1795 from Pseudomonas aeruginosa (460 aa) FASTA scores: opt: 1197, E(): 1.2e-68, (41.65% identity in 468 aa overlap); P21888|SYC_ECOLI P21888|CYSS|B0526 from Escherichia coli strain K12 (461 aa), FASTA scores: opt: 1189, E(): 4e-68, (43.0% identity in 463 aa overlap); etc. Belongs to class-I aminoacyl-tRNA synthetase family. Strongly similar to methionyl-tRNA synthetase.; Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase) 4023803 cysS1 888628 cysS1 Mycobacterium tuberculosis H37Rv Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase) YP_177992.1 4022394 R 83332 CDS NP_218098.1 15610717 888221 complement(4023868..4024347) 1 NC_000962.3 Rv3581c, (MT3687, MTCY06G11.28c), len: 159 aa. Probable ispF, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, equivalent to Q9CCW5|ML0322 putative 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Mycobacterium leprae (158 aa), FASTA scores: opt: 830, E(): 2.9e-47, (79.1% identity in 158 aa overlap). Also highly similar to others e.g. Q9L0Q7|ISPF_STRCO|SCD8A.07 from Streptomyces coelicolor (170 aa), FASTA scores: opt: 585,E(): 2.9e-31, (56.5% identity in 154 aa overlap); Q9PDT5|ISPF_XYLFA|XF1294 from Xylella fastidiosa (176 aa),FASTA scores: opt: 398, E(): 4.6e-19, (44.9% identity in 156 aa overlap); Q08113|ISDF_RHOCA|ISPDF from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (379 aa), FASTA scores: opt: 387, E(): 4.5e-18, (42.85% identity in 154 aa overlap) (only similar with C-terminal end of this bifunctional protein ISPD and ISPF); Q06756|ISPF_BACSU from Bacillus subtilis (158 aa), FASTA scores: opt: 367, E(): 4.5e-17, (41.2% identity in 153 aa overlap); etc. Belongs to the IspF family.; Probable 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase IspF (MECPS) 4024347 ispF 888221 ispF Mycobacterium tuberculosis H37Rv Probable 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase IspF (MECPS) NP_218098.1 4023868 R 83332 CDS NP_218099.1 15610718 887787 complement(4024344..4025039) 1 NC_000962.3 Rv3582c, (MT3688, MTCY06G11.29c), len: 231 aa. ispD,4-diphosphocytidyl-2C-methyl-D-erythritol synthase ,equivalent to Q9CCW6|ML0321 putative 4-diphosphocytidyl-2C-methyl-D-erythritol synthase from Mycobacterium leprae (241 aa), FASTA scores: opt: 694, E(): 1.7e-35, (66.95% identity in 236 aa overlap). Also highly similar to others e.g. Q9L0Q8|ISPD_STRCO|SCD8A.06 from Streptomyces coelicolor (270 aa), FASTA scores: opt: 537,E(): 7.5e-26, (43.4% identity in 242 aa overlap); P74323|ISPD_SYNY3|SLR0951 from Synechocystis sp. strain PCC 6803 (230 aa), FASTA scores: opt: 410, E(): 3.8e-18,(36.15% identity in 224 aa overlap); Q9KGF8|ISPD_BACHD|BH0107 from Bacillus halodurans (228 aa) FASTA scores: opt: 367, E(): 1.6e-15, (34.65% identity in 228 aa overlap); Q08113|ISDF_RHOCA|ISPDF from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (379 aa)FASTA scores: opt: 359, E(): 7.8e-15, (34.1% identity in 223 aa overlap) (only similar with N-terminus of this bifunctional protein ISPD and ISPF); Q46893|ISPD_ECOLI|B2747 from Escherichia coli strain K12 (235 aa), FASTA scores: opt: 336, E(): 1.3e-13, (33.65% identity in 223 aa overlap); etc. Belongs to the ISPD family.; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase IspD (MEP cytidylyltransferase) (MCT) 4025039 ispD 887787 ispD Mycobacterium tuberculosis H37Rv 4-diphosphocytidyl-2C-methyl-D-erythritol synthase IspD (MEP cytidylyltransferase) (MCT) NP_218099.1 4024344 R 83332 CDS NP_218100.1 15610719 887854 complement(4025056..4025544) 1 NC_000962.3 Rv3583c, (MTV024.01c, MTCY06G11.30c), len: 162 aa. Possible transcriptional factor, identical to Q9CCW7|ML0320 putative transcription factor from Mycobacterium leprae (165 aa), FASTA scores: opt: 1004, E(): 6.1e-56, (97.55% identity in 162 aa overlap); and Q9ZBM8|MLCB1450.01c putative transcriptional regulator from Mycobacterium leprae (94 aa), FASTA scores: opt: 600, E(): 6e-31, (97.85% identity in 94 aa overlap). Also highly similar to others e.g. Q9L0Q9|SCD8A.05 from Streptomyces coelicolor (160 aa),FASTA scores: opt: 878, E(): 4.3e-48, (85.0% identity in 160 aa overlap); Q9K600|BH3935 from Bacillus halodurans (153 aa) FASTA scores: opt: 383, E(): 3.1e-17, (36.4% identity in 151 aa overlap); Q9KD36|BH1383 from Bacillus halodurans (164 aa) FASTA scores: opt: 305, E(): 2.4e-12,(33.55% identity in 164 aa overlap); etc.; Possible transcription factor 4025544 887854 Rv3583c Mycobacterium tuberculosis H37Rv Possible transcription factor NP_218100.1 4025056 R 83332 CDS NP_218101.1 15610720 887254 4025830..4026378 1 NC_000962.3 Rv3584, (MTV024.02), len: 182 aa. Possible lpqE,conserved lipoprotein, equivalent to Q9ZBM7|MLCB1450.02|LPQE|ML0319 putative lipoprotein from Mycobacterium leprae (183 aa), FASTA scores: opt: 722, E(): 6.2e-37, (63.45% identity in 175 aa overlap). Also similar in part to Q9KK69 exported protein 996A010 (fragment) from Mycobacterium avium (41 aa), FASTA scores: opt: 180, E(): 0.00012, (69.25% identity in 39 aa overlap); and Q9L0R0|SCD8A.04c putative lipoprotein from Streptomyces coelicolor (241 aa), FASTA scores: opt: 127, E(): 0.86,(27.15% identity in 173 aa overlap). Equivalent to AAK48048 from Mycobacterium tuberculosis strain CDC1551 (238 aa) but shorter 56 aa. Contains probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved lipoprotein LpqE 4026378 lpqE 887254 lpqE Mycobacterium tuberculosis H37Rv Possible conserved lipoprotein LpqE NP_218101.1 4025830 D 83332 CDS NP_218102.1 15610721 887287 4026444..4027886 1 NC_000962.3 Rv3585, (MTV024.03), len: 480 aa. Probable radA, DNA repair protein (see citation below), similar to many e.g. Q9X8L5|SCE94.02 from Streptomyces coelicolor (469 aa),FASTA scores: opt: 1607, E(): 3.1e-84, (56.15% identity in 454 aa overlap); Q9JV51|RADA|NMA0992 from Neisseria meningitidis (serogroup A) (459 aa), FASTA scores: opt: 1275, E(): 2.5e-65, (45.0% identity in 458 aa overlap); and Q9K040|RADA|NMB0782 from Neisseria meningitidis (serogroup B) (459 aa), FASTA scores: opt: 1269, E(): 5.4e-65, (44.5% identity in 456 aa overlap); P37572|RADA_BACSU|SMS from Bacillus subtilis (458 aa), FASTA scores: opt: 1204, E(): 2.7e-61, (39.55% identity in 455 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the RadA family.; DNA repair protein RadA (DNA repair protein SMS) 4027886 radA 887287 radA Mycobacterium tuberculosis H37Rv DNA repair protein RadA (DNA repair protein SMS) NP_218102.1 4026444 D 83332 CDS NP_218103.1 15610722 887485 4027891..4028967 1 NC_000962.3 Rv3586, (MTV024.04), len: 358 aa. Conserved hypothetical protein, highly similar to Q9X8L6|SCE94.03 putative DNA-binding protein from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1338, E(): 5e-75, (59.95% identity in 347 aa overlap); P37573|YACK_BACSU hypothetical 40.7 KDA protein from Bacillus subtilis (360 aa), FASTA scores: opt: 875, E(): 1.4e-46, (42.15% identity in 344 aa overlap); Q9KGG0|BH0105 hypothetical protein from Bacillus halodurans (357 aa), FASTA scores: opt: 844, E(): 1.1e-44,(40.3% identity in 350 aa overlap); Q9WY43|TM0200 conserved hypothetical protein from Thermotoga maritima (357 aa),FASTA scores: opt: 735, E(): 5.7e-38, (39.4% identity in 353 aa overlap). Also some similarity with other proteins. Contains probable coiled-coil from 144 to 179.; hypothetical protein 4028967 887485 Rv3586 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218103.1 4027891 D 83332 CDS NP_218104.1 15610723 888057 complement(4028968..4029762) 1 NC_000962.3 Rv3587c, (MTV024.05c), len: 264 aa. Probable conserved membrane protein, equivalent to Q9CBJ2|ML1918 hypothetical membrane protein from Mycobacterium leprae (263 aa), FASTA scores: opt: 1438, E(): 2.4e-57, (77.55% identity in 267 aa overlap). Contains hydrophobic stretch in N-terminus; possible signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein 4029762 888057 Rv3587c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_218104.1 4028968 R 83332 CDS NP_218105.1 15610724 887836 complement(4029871..4030494) 1 NC_000962.3 Rv3588c, (MTV024.06c), len: 207 aa. CanB,Beta-carbonic anhydrase, proven biochemically (See Suarez Covarrubias et al. 2005) similar to others e.g. Q9CBJ1|ML1919 putative carbonic anhydrase from Mycobacterium leprae (213 aa), FASTA scores: opt: 1160,E(): 3.1e-66, (84.55% identity in 207 aa overlap). Also similar to many e.g. Q9X903|SCH35.03 from Streptomyces coelicolor (207 aa), FASTA scores: opt: 689, E(): 1.6e-36,(53.85% identity in 195 aa overlap); Q9RS89|DR2238 from Deinococcus radiodurans (264 aa), FASTA scores: opt: 451,E(): 2e-21, (39.7% identity in 189 aa overlap); Q39589|beta-CA1 from Chlamydomonas reinhardtii (267 aa) FASTA scores: opt: 419, E(): 2.1e-19, (36.55% identity in 197 aa overlap); etc. Contains PS00704 and PS00705 Prokaryotic-type carbonic anhydrases signature 1 and 2. Belongs to the plant and prokaryotic carbonic anhydrase family.; Beta-carbonic anhydrase CanB 4030494 canB 887836 canB Mycobacterium tuberculosis H37Rv Beta-carbonic anhydrase CanB NP_218105.1 4029871 R 83332 CDS NP_218106.1 15610725 886639 4030493..4031407 1 NC_000962.3 Rv3589, (MTV024.07), len: 304 aa. Probable mutY,adenine glycosylase (see citation below), equivalent to Q9CBJ0|MUTY|ML1920 probable DNA glycosylase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1592,E(): 2.6e-94, (74.9% identity in 303 aa overlap). Also similar to many DNA glycosylases (generally adenine glycosylases) e.g. Q9S6T7|SCE94.06 from Streptomyces coelicolor (308 aa), FASTA scores: opt: 965, E(): 2.6e-54,(50.5% identity in 297 aa overlap); Q9S6G1|MUTY from Streptomyces antibioticus (307 aa), FASTA scores: opt: 901,E(): 3.1e-50, (48.5% identity in 303 aa overlap); Q9HPQ6|MUTY|VNG1520G from Halobacterium sp. strain NRC-1 (312 aa), FASTA scores: opt: 566, E(): 7.2e-29, (39.85% identity in 296 aa overlap); BAB53965|MLL7523 from Rhizobium loti (Mesorhizobium loti) (396 aa), FASTA scores: opt: 511, E(): 2.8e-25, (39.65% identity in 237 aa overlap); Q05869|MUTY_SALTY|MUTB from Salmonella typhimurium (350 aa), FASTA scores: opt: 421, E(): 3.8e-20,(35.2% identity in 227 aa overlap); etc. Could belong to the nth/MUTY family.; Probable adenine glycosylase MutY 4031407 mutY 886639 mutY Mycobacterium tuberculosis H37Rv Probable adenine glycosylase MutY NP_218106.1 4030493 D 83332 CDS YP_177993.1 57117129 887874 complement(4031404..4033158) 1 NC_000962.3 Rv3590c, (MTV024.08c, MTCY6F7.04), len: 584 aa. PE_PGRS58, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to e.g. O53439|Rv1091|MTV017.44 (853 aa), FASTA scores: opt: 2005, E(): 1.4e-70, (54.95% identity in 646 aa overlap).; PE-PGRS family protein PE_PGRS58 4033158 PE_PGRS58 887874 PE_PGRS58 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS58 YP_177993.1 4031404 R 83332 CDS NP_218108.1 15610727 886316 complement(4033269..4034042) 1 NC_000962.3 Rv3591c, (MTCY6F7.03), len: 257 aa. Possible hydrolase, equivalent to Q9CBI9|ML1921 hypothetical protein from Mycobacterium leprae (256 aa) FASTA scores: opt: 1421,E(): 5.6e-83, (78.5% identity in 251 aa overlap). Also similar to others e.g. Q9K3V0|SCD10.27 putative hydrolase from Streptomyces coelicolor (352 aa), FASTA scores: opt: 193, E(): 5.2e-05, (33.35% identity in 270 aa overlap); O33745|STTC thioesterase from Streptomyces sp (308 aa) FASTA scores: opt: 242, E(): 3.6e-08, (30.35% identity in 270 aa overlap); Q9RK95|SCF1.09 putative hydrolase from Streptomyces coelicolor (258 aa), FASTA scores: opt: 239,E(): 4.9e-08, (30.75% identity in 247 aa overlap); Q9HZ14|PA3226 probable hydrolase from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 226, E(): 3.4e-07,(26.6% identity in 252 aa overlap); Q9HPT9|est|VNG1474G carboxylesterase from Halobacterium sp. strain NRC-1 (274 aa), FASTA scores: opt: 215, E(): 1.7e-06, (26.95% identity in 256 aa overlap); etc.; Possible hydrolase 4034042 886316 Rv3591c Mycobacterium tuberculosis H37Rv Possible hydrolase NP_218108.1 4033269 R 83332 CDS NP_218109.1 15610728 886278 4034057..4034374 1 NC_000962.3 Rv3592, (MTCY6F7.02c), len: 105 aa. Possible mhuD,heme-degrading protein, equivalent to Q9CBI8|ML1922 hypothetical protein from Mycobacterium leprae (105 aa) FASTA scores: opt: 591, E(): 2.5e-34, (84.6% identity in 104 aa overlap). Shows some similarity with other bacterial hypothetical proteins e.g. Q9RXN8|DR0272 from Deinococcus radiodurans (109 aa), FASTA scores: opt: 178, E(): 1e-05,(34.3% identity in 102 aa overlap); P38049|YHGC_BACSU from Bacillus subtilis (166 aa) FASTA scores: opt: 175, E(): 2.4e-05, (40.85% identity in 71 aa overlap); Q9K649|BH3883 from Bacillus halodurans (102 aa) FASTA scores: opt: 162,E(): 0.00012, (33.75% identity in 80 aa overlap); etc.; Possible heme degrading protein MhuD 4034374 mhuD 886278 mhuD Mycobacterium tuberculosis H37Rv Possible heme degrading protein MhuD NP_218109.1 4034057 D 83332 CDS NP_218110.1 15610729 886290 4034352..4035710 1 NC_000962.3 Rv3593, (MTCY6F7.01c), len: 452 aa. Probable lpqF,conserved lipoprotein, equivalent to Q9CBI7|MPQF|ML1923 probale secreted protein from Mycobacterium leprae (454 aa), FASTA scores: opt: 2465, E(): 5.7e-144, (79.15% identity in 451 aa overlap). Also similar to Q9KJ91 hypothetical 47.1 KDA protein from Streptomyces clavuligerus (430 aa), FASTA scores: opt: 609, E(): 5.2e-30, (30.3% identity in 350 aa overlap); and some similarity with putative beta-lactamases e.g. Q9RYR7|DRA0241 beta lactamase-related protein from Deinococcus radiodurans (499 aa), FASTA scores: opt: 322,E(): 2.5e-12, (28.25% identity in 322 aa overlap). Equivalent to AAK48057 from Mycobacterium tuberculosis strain CDC1551 (438 aa) but longer 14 aa. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; Probable conserved lipoprotein LpqF 4035710 lpqF 886290 lpqF Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqF NP_218110.1 4034352 D 83332 CDS NP_218111.1 15610730 885659 4035857..4036684 1 NC_000962.3 Rv3594, (MTCY07H7B.28c), len: 275 aa. Hypothetical protein, highly similar in part with Q9ZX49|GP29 from Mycobacteriophage TM4 (547 aa), FASTA scores: opt: 526,E(): 1.3e-25, (46.25% identity in 186 aa overlap); and Q9FZS0|LYSA|GP2 from Mycobacterium phage Ms6 (384 aa) FASTA scores: opt: 147, E(): 0.064, (33.35% identity in 84 aa overlap).; hypothetical protein 4036684 885659 Rv3594 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218111.1 4035857 D 83332 CDS YP_177994.1 57117130 885464 complement(4036731..4038050) 1 NC_000962.3 Rv3595c, (MTCY07H7B.27), len: 439 aa. PE_PGRS59,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below),similar to many e.g. O53439|Rv1091|MTV017.44 (853 aa),FASTA scores: opt: 1644, E(): 1.2e-57, (58.75% identity in 492 aa overlap).; PE-PGRS family protein PE_PGRS59 4038050 PE_PGRS59 885464 PE_PGRS59 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS59 YP_177994.1 4036731 R 83332 CDS YP_177995.1 57117131 885104 complement(4038158..4040704) 1 NC_000962.3 Rv3596c, (MTCY07H7B.26), len: 848 aa. Probable clpC1, ATP-dependent protease ATP-binding subunit,equivalent to P24428|CLPC_MYCLE probable ATP-dependent CLP protease ATP-binding subunit from Mycobacterium leprae (848 aa) (see Misra et al., 1996), FASTA scores: opt: 5286, E(): 0, (97.15% identity in 845 aa overlap). Also highly similar to members of the clpA/clpB family e.g. Q9S6T8|SCE94.24c from Streptomyces coelicolor (841 aa) FASTA scores: opt: 4399, E(): 0, (81.0% identity in 848 aa overlap); Q9KGG2|CLPC|BH0103 from Bacillus halodurans (813 aa), FASTA scores: opt: 3279, E(): 3.8e-173, (61.9% identity in 808 aa overlap); Q55662|CLPC|SLL0020 from Synechocystis sp. strain PCC 6803 (821 aa), FASTA scores: opt: 3201, E(): 7.6e-169,(60.5% identity in 820 aa overlap); P51332|CLPC_PORPU from Porphyra purpurea (821 aa), FASTA scores: opt: 3045, E(): 3e-160, (57.65% identity in 817 aa overlap); P37571|CLPC_BACSU|MECB from Bacillus subtilis (810 aa),FASTA scores: opt: 2969, E(): 4.6e-156, (61.15% identity in 811 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Note that previously known as clpC. Belongs to the CLPA/CLPB family, CLPC subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable ATP-dependent protease ATP-binding subunit ClpC1 4040704 clpC1 885104 clpC1 Mycobacterium tuberculosis H37Rv Probable ATP-dependent protease ATP-binding subunit ClpC1 YP_177995.1 4038158 R 83332 CDS NP_218114.1 15610733 885580 complement(4040981..4041319) 1 NC_000962.3 Rv3597c, (MTCY07H7B.25), len: 112 aa. Lsr2,H-NS-like protein, identical to P24094|LSR2_MYCLE|ML0234 LSR2 protein precursor (15 KDA antigen) (A15) from Mycobacterium leprae (112 aa), FASTA scores: opt: 698, E(): 6.7e-37, (92.85% identity in 112 aa overlap). Also highly similar to others e.g. Q9X8N1|SCE94.26c from Streptomyces coelicolor (111 aa), FASTA scores: opt: 379, E(): 4.4e-17,(58.05% identity in 112 aa overlap); Q9ETI2|LSR2 from Corynebacterium equii (Rhodococcus equi) (119 aa), FASTA scores: opt: 328, E(): 6.9e-14, (47.5% identity in 120 aa overlap); and Q9RKK8|SCD25.12c from Streptomyces coelicolor (105 aa), FASTA scores: opt: 293, E(): 9.4e-12, (47.75% identity in 111 aa overlap).; Iron-regulated H-NS-like protein Lsr2 4041319 lsr2 885580 lsr2 Mycobacterium tuberculosis H37Rv Iron-regulated H-NS-like protein Lsr2 NP_218114.1 4040981 R 83332 CDS NP_218115.1 15610734 885574 complement(4041423..4042940) 1 NC_000962.3 Rv3598c, (MTCY07H7B.24), len: 505 aa. Probable lysS,lysyl-tRNA synthetase 1, equivalent to P46861|SYK_MYCLE|LYSS|ML0233 lysyl-tRNA synthetase from Mycobacterium leprae (507 aa), FASTA scores: opt: 2835,E(): 4.5e-172, (85.45% identity in 501 aa overlap); and similar with C-terminal part of Q9CC23|LYSX|ML1393 C-term lysyl-tRNA synthase from Mycobacterium leprae (1039 aa) FASTA scores: opt: 1257, E(): 7.6e-72, (44.55% identity in 505 aa overlap). Also similar to others e.g. P37477|SYK_BACSU|LYSS from Bacillus subtilis (499 aa) FASTA scores: opt: 1294, E(): 1.9e-74, (42.35% identity in 498 aa overlap); Q9RHV9|SYK_BACST|LYSS from Bacillus stearothermophilus (494 aa), FASTA scores: opt: 1258, E(): 3.5e-72, (41.15% identity in 498 aa overlap); Q9PEB6|SYK_XYLFA|LYSS|XF1112 from Xylella fastidiosa (506 aa), FASTA scores: opt: 1228, E(): 2.9e-70, (43.05% identity in 495 aa overlap); etc. Also similar to P94974|SYK2_MYCTU|LYSS2|LYSX|Rv1640c|MTCY06H11.04c lysyl-tRNA synthetase 2 from Mycobacterium tuberculosis (1172 aa), FASTA scores: opt: 1295, E(): 3.3e-74, (45.65% identity in 506 aa overlap). Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family.; Lysyl-tRNA synthetase 1 LysS (lysine--tRNA ligase 1) (LysRS 1) (lysine translase) 4042940 lysS 885574 lysS Mycobacterium tuberculosis H37Rv Lysyl-tRNA synthetase 1 LysS (lysine--tRNA ligase 1) (LysRS 1) (lysine translase) NP_218115.1 4041423 R 83332 CDS NP_218116.1 15610735 887149 complement(4042952..4043035) 1 NC_000962.3 Rv3599c, (MTCY07H7B.23), len: 27 aa. Hypothetical unknown protein.; Hypothetical short protein 4043035 887149 Rv3599c Mycobacterium tuberculosis H37Rv Hypothetical short protein NP_218116.1 4042952 R 83332 CDS NP_218117.1 15610736 885572 complement(4043041..4043859) 1 NC_000962.3 Rv3600c, (MTCY07H7B.22), len: 272 aa. Conserved protein, identical to Q9CD56|ML0232 hypothetical protein from Mycobacterium leprae (274 aa), FASTA scores: opt: 1585, E(): 1.3e-92, (90.5% identity in 274 aa overlap). Also highly similar to others e.g. Q9X8N6|SCE94.31c from Streptomyces coelicolor (265 aa) FASTA scores: opt: 878,E(): 3.9e-48, (51.5% identity in 268 aa overlap); and Q9KGH5|BH0086 from Bacillus halodurans (254 aa), FASTA scores: opt: 611, E(): 2.4e-31, (37.5% identity in 264 aa overlap). And similar to various bacterial proteins e.g. Q9F985 putative 32 KDA replication protein from Bacillus stearothermophilus (258 aa), FASTA scores: opt: 594, E(): 2.8e-30, (37.45% identity in 267 aa overlap); P37564|YACB_BACSU from Bacillus subtilis (233 aa), FASTA scores: opt: 522, E(): 8.8e-26, (38.95% identity in 213 aa overlap); Q9RX54|DR0461 conserved hypothetical protein from Deinococcus radiodurans (262 aa), FASTA scores: opt: 503,E(): 1.5e-24, (38.45% identity in 268 aa overlap); etc.; hypothetical protein 4043859 885572 Rv3600c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218117.1 4043041 R 83332 CDS NP_218118.1 15610737 885596 complement(4043862..4044281) 1 NC_000962.3 Rv3601c, (MTCY07H7B.21), len: 139 aa. Probable panD,aspartate 1-decarboxylase, identical to Q9CD57|PAND|ML0231 putative aspartate-1-decarboxylase from Mycobacterium leprae (142 aa), FASTA scores: opt: 733, E(): 5.5e-41,(82.85% identity in 140 aa overlap). Also highly similar to many e.g. CAC44328|PAND from Streptomyces coelicolor (139 aa), FASTA scores: opt: 578, E(): 6.4e-31, (75.0% identity in 120 aa overlap); Q9X4N0|PAND from Corynebacterium glutamicum (Brevibacterium flavum) (136 aa), FASTA scores: opt: 506, E(): 3e-26, (62.2% identity in 135 aa overlap); P52999|PAND_BACSU from Bacillus subtilis (127 aa) FASTA scores: opt: 421, E(): 9.6e-21, (54.75% identity in 123 aa overlap); P31664|PAND_ECOLI|B0131 from Escherichia coli strain K12 (126 aa), FASTA scores: opt: 388, E(): 1.3e-18,(50.45% identity in 113 aa overlap); etc.; Probable aspartate 1-decarboxylase precursor PanD (aspartate alpha-decarboxylase) 4044281 panD 885596 panD Mycobacterium tuberculosis H37Rv Probable aspartate 1-decarboxylase precursor PanD (aspartate alpha-decarboxylase) NP_218118.1 4043862 R 83332 CDS NP_218119.1 15610738 885459 complement(4044281..4045210) 1 NC_000962.3 Rv3602c, (MTCY07H7B.20), len: 309 aa. panC,pantoate--beta-alanine ligase, equivalent to O69524|PANC_MYCLE|ML0230|MLCB2548.01c pantoate--beta-alanine ligase from Mycobacterium leprae (313 aa), FASTA scores: opt: 1541, E(): 3.4e-84, (82.15% identity in 297 aa overlap). Also similar to others e.g. O67891|PANC_AQUAE|AQ_2132 from Aquifex aeolicus (282 aa) FASTA scores: opt: 774, E(): 8.6e-39, (46.9% identity in 273 aa overlap); Q9HV69|PANC_PSEAE|PA4730 from Pseudomonas aeruginosa (283 aa), FASTA scores: opt: 770, E(): 1.5e-38,(51.45% identity in 276 aa overlap); Q9A6C8|CC2166 from Caulobacter crescentus (285 aa), FASTA scores: opt: 744,E(): 5.2e-37, (47.75% identity in 268 aa overlap); P31663|PANC_ECOLI|B0133 from Escherichia coli strain K12 (283 aa), FASTA scores: opt: 695, E(): 4.1e-34, (46.1% identity in 271 aa overlap); etc. Belongs to the pantothenate synthetase family.; Pantoate--beta-alanine ligase PanC (pantothenate synthetase) (pantoate activating enzyme) 4045210 panC 885459 panC Mycobacterium tuberculosis H37Rv Pantoate--beta-alanine ligase PanC (pantothenate synthetase) (pantoate activating enzyme) NP_218119.1 4044281 R 83332 CDS NP_218120.1 15610739 885583 complement(4045207..4046118) 1 NC_000962.3 Rv3603c, (MTCY07H7B.19), len: 303 aa. Conserved hypothetical ala-, leu-rich protein, identical except at N-terminus (really different) to AAK48066|MT3708 chalcone/stilbene synthase family protein from Mycobacterium tuberculosis strain CDC1551 (361 aa) FASTA scores: opt: 1742, E(): 8.3e-95, (100.0% identity in 275 aa overlap). Equivalent to O69525|MLCB2548.02c|ML0229 hypothetical 32.7 KDA protein from Mycobacterium leprae (309 aa), FASTA scores: opt: 947, E(): 2.4e-48, (67.85% identity in 311 aa overlap). Also highly similar to Q9X845|SCE126.02c hypothetical 42.2 KDA protein from Streptomyces coelicolor (420 aa), FASTA scores: opt: 683,E(): 8.5e-33, (49.3% identity in 284 aa overlap).; Conserved hypothetical alanine and leucine rich protein 4046118 885583 Rv3603c Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine and leucine rich protein NP_218120.1 4045207 R 83332 CDS NP_218121.2 57117132 885232 complement(4046303..4047496) 1 NC_000962.3 Rv3604c, (MTCY07H7B.18), len: 397 aa. Probable conserved ala-, arg-, pro-rich transmembrane protein,equivalent to O69526|MLCB2548.03c|ML0228 putative membrane protein from Mycobacterium leprae (432 aa), FASTA scores: opt: 869, E(): 2.9e-31, (59.7% identity in 432 aa overlap). Contains two possible membrane-spanning domains. N-terminus shortened since first submission (previously 462 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein rich in alanine and arginine and proline 4047496 885232 Rv3604c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein rich in alanine and arginine and proline NP_218121.2 4046303 R 83332 CDS NP_218122.1 15610741 885844 complement(4047705..4048181) 1 NC_000962.3 Rv3605c, (MTCY07H7B.17), len: 158 aa. Probable conserved secreted or membrane protein, identical to O69527|MLCB2548.04c|ML0227 putative membrane protein from Mycobacterium leprae (158 aa), FASTA scores: opt: 944, E(): 2.6e-56, (85.45% identity in 158 aa overlap). Also similar to other proteins e.g. Q9X8I2|SCE9.09 possible secreted protein from Streptomyces coelicolor (162 aa), FASTA scores: opt: 174, E(): 9.2e-05, (31.25% identity in 128 aa overlap); etc. Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved secreted protein 4048181 885844 Rv3605c Mycobacterium tuberculosis H37Rv Probable conserved secreted protein NP_218122.1 4047705 R 83332 CDS NP_218123.1 15610742 885848 complement(4048181..4048747) 1 NC_000962.3 Rv3606c, (MTCY07H7B.16), len: 188 aa. Probable folK,2-amino-4-hydroxy-6-hydroxymethyldihydropterine pyrophosphokinase, equivalent to O69528|HPPK_MYCLE|folk|ML0226\MLCB2548.05c 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase from Mycobacterium leprae (191 aa) FASTA scores: opt: 772, E(): 1.2e-44, (63.15% identity in 190 aa overlap). Also similar to many e.g. P71512|HPPK_METEX|folk|FOLA from Methylobacterium extorquens (158 aa), FASTA scores: opt: 292, E(): 1.4e-12,(36.85% identity in 171 aa overlap); O33726|HPPK_STRPY|folk|SPY1100 from Streptococcus pyogenes (166 aa), FASTA scores: opt: 234, E(): 1.1e-08, (34.3% identity in 175 aa overlap); Q9X8I1|SCE9.08 from Streptomyces coelicolor (203 aa), FASTA scores: opt: 232,E(): 1.7e-08, (43.25% identity in 185 aa overlap); P26281|HPPK_ECOLI|folk|B0142 from Escherichia coli strain K12 (158 aa), FASTA scores: opt: 198, E(): 2.6e-06, (32.85% identity in 143 aa overlap); etc. Belongs to the HppK family.; 2-amino-4-hydroxy-6- hydroxymethyldihydropteridinepyrophosphokinase FolK (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (HPPK) (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase) (PPPK) (2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase) (7,8-dihydro-6-hydroxymethylpterin-diphosphokinase) (6-hydroxymethyl-7,8-dihydropterin diphosphokinase) 4048747 folK 885848 folK Mycobacterium tuberculosis H37Rv 2-amino-4-hydroxy-6- hydroxymethyldihydropteridinepyrophosphokinase FolK (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (HPPK) (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase) (PPPK) (2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase) (7,8-dihydro-6-hydroxymethylpterin-diphosphokinase) (6-hydroxymethyl-7,8-dihydropterin diphosphokinase) NP_218123.1 4048181 R 83332 CDS YP_177996.1 57117133 885345 complement(4048744..4049145) 1 NC_000962.3 Rv3607c, (MTCY07H7B.15), len: 133 aa. Probable folB,dihydroneopterin aldolase, equivalent to O69529|FOLB_MYCLE|ML0225|MLCB2548.06c probable dihydroneopterin aldolase from Mycobacterium leprae (132 aa), FASTA scores: opt: 673, E(): 5.1e-37, (74.8% identity in 131 aa overlap). Also similar to many e.g. Q9X8I0|FOLB_STRCO|SCE9.07 from Streptomyces coelicolor (119 aa), FASTA scores: opt: 334, E(): 4.5e-15, (46.15% identity in 117 aa overlap); P74342|FOLB_SYNY3|SLR1626 from Synechocystis sp. strain PCC 6803 (118 aa) FASTA scores: opt: 287, E(): 5e-12, (38.45% identity in 117 aa overlap); P28823|FOLB_BACSU|FOLA from Bacillus subtilis (120 aa),FASTA scores: opt: 283, E(): 9.2e-12, (39.0% identity in 118 aa overlap); etc. Belongs to the DHNA family. Note that previously known as folX.; Probable dihydroneopterin aldolase FolB (DHNA) 4049145 folB 885345 folB Mycobacterium tuberculosis H37Rv Probable dihydroneopterin aldolase FolB (DHNA) YP_177996.1 4048744 R 83332 CDS YP_177997.1 57117134 885831 complement(4049138..4049980) 1 NC_000962.3 Rv3608c, (MTCY07H7B.14), len: 280 aa. Probable folP1, dihydropteroate synthase 1, equivalent to O69530|FOLP (alias Q9S0T0|FOLP and Q9R2U9|FOLP) dihydroneopterin aldolase from Mycobacterium leprae (284 aa), FASTA scores: opt: 1418, E(): 7.2e-77, (76.75% identity in 284 aa overlap). Also highly similar to many e.g. Q9X8H8|SCE9.05 from Streptomyces coelicolor (288 aa),FASTA scores: opt: 953, E(): 2.4e-49, (56.0% identity in 266 aa overlap); Q9A3I0|CC3224 from Caulobacter crescentus (274 aa), FASTA scores: opt: 682, E(): 2.6e-33, (45.5% identity in 268 aa overlap); P73248|DHPS_SYNY3|FOLP|SLR2026 from Synechocystis sp. strain PCC 6803 (289 aa), FASTA scores: opt: 665, E(): 2.7e-32, (44.55% identity in 265 aa overlap); P26282|DHPS_ECOLI|FOLP|B3177 from Escherichia coli strain K12 (282 aa), FASTA scores: opt: 642, E(): 6.1e-31, (41.95% identity in 274 aa overlap); etc. Contains PS00792 Dihydropteroate synthase signature 1, PS00793 Dihydropteroate synthase signature 2. Similar to other species DHPS.; Dihydropteroate synthase 1 FolP (DHPS 1) (dihydropteroate pyrophosphorylase 1) (dihydropteroate diphosphorylase 1) 4049980 folP1 885831 folP1 Mycobacterium tuberculosis H37Rv Dihydropteroate synthase 1 FolP (DHPS 1) (dihydropteroate pyrophosphorylase 1) (dihydropteroate diphosphorylase 1) YP_177997.1 4049138 R 83332 CDS NP_218126.1 15610745 885346 complement(4049977..4050585) 1 NC_000962.3 Rv3609c, (MTCY07H7B.13), len: 202 aa. Probable folE (alternate gene name: gchA), GTP cyclohydrolase I,equivalent to O69531|GCH1_MYCLE|FOLE|ML0223|MLCB2548.08c GTP cyclohydrolase I from Mycobacterium leprae (205 aa) FASTA scores: opt: 1112, E(): 3.8e-63, (81.95% identity in 205 aa overlap). Also highly similar to many e.g. Q9X8I3|GCH1_STRCO|FOLE|SCE9.10c from Streptomyces coelicolor (201 aa), FASTA scores: opt: 873, E(): 4.2e-48,(67.4% identity in 187 aa overlap); Q9KCC7|MTRA|BH1646 from Bacillus halodurans (188 aa), FASTA scores: opt: 757, E(): 8.1e-41, (62.3% identity in 183 aa overlap); P19465|GCH1_BACSU|FOLE|MTRA from Bacillus subtilis (190 aa), FASTA scores: opt: 750, E(): 2.3e-40, (58.95% identity in 190 aa overlap); etc. Contains PS00860 GTP cyclohydrolase I signature 2. Belongs to the GTP cyclohydrolase I family.; GTP cyclohydrolase I FolE (GTP-ch-I) 4050585 folE 885346 folE Mycobacterium tuberculosis H37Rv GTP cyclohydrolase I FolE (GTP-ch-I) NP_218126.1 4049977 R 83332 CDS NP_218127.1 15610746 885732 complement(4050601..4052883) 1 NC_000962.3 Rv3610c, (MT3714, MTCY07H7B.12), len: 760 aa. FtsH,membrane-bound protease (cell division protein) (see citations below), equivalent to Q9CD58|FTSH_MYCLE|ML0222 (alias O69532|FTSH) cell division protein FTSH homolog from Mycobacterium leprae (787 aa), FASTA scores: opt: 4388,E(): 9.6e-205, (87.2% identity in 790 aa overlap). Also highly similar to many FTSH proteins e.g. O52395|FTSH from Mycobacterium smegmatis (769 aa), FASTA scores: opt: 3976,E(): 7.6e-185, (82.4% identity in 761 aa overlap); Q9X8I4|SCE9.11c from Streptomyces coelicolor (668 aa),FASTA scores: opt: 2417, E(): 1.4e-109, (57.2% identity in 668 aa overlap); P72991|FTH4_SYNY3|SLR1604 from Synechocystis sp. strain PCC 6803 (616 aa), FASTA scores: opt: 1926, E(): 7.2e-86, (49.35% identity in 612 aa overlap); P28691|FTSH_ECOLI|HFLB|MRSC|TOLZ|B3178 from Escherichia coli strain K12 (644 aa), FASTA scores: opt: 1859, E(): 1.3e-82, (48.95% identity in 605 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00674 AAA-protein family signature. Belongs to the AAA family of ATPases and peptidase family M41 (zinc metalloprotease). Cofactor: binds one zinc ion (potential). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Membrane-bound protease FtsH (cell division protein) 4052883 ftsH 885732 ftsH Mycobacterium tuberculosis H37Rv Membrane-bound protease FtsH (cell division protein) NP_218127.1 4050601 R 83332 CDS NP_218128.1 15610747 885469 4052950..4053603 1 NC_000962.3 Rv3611, (MTCY07H7B.11c), len: 217 aa. Hypothetical unknown arg-, pro-rich protein. Possible ORF containing several direct repeats.; Hypothetical arginine and proline rich protein 4053603 885469 Rv3611 Mycobacterium tuberculosis H37Rv Hypothetical arginine and proline rich protein NP_218128.1 4052950 D 83332 CDS NP_218129.1 15610748 885381 complement(4053518..4053847) 1 NC_000962.3 Rv3612c, (MTCY07H7B.10), len: 109 aa. Conserved hypothetical protein. Residues 58 to 81 highly similar to N-terminal part of AAK46718|MT2424 hypothetical 3.9 KDA protein from Mycobacterium tuberculosis strain CDC1551 (36 aa), FASTA scores: opt: 108, E(): 0.38, (69.25% identity in 26 aa overlap).; hypothetical protein 4053847 885381 Rv3612c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218129.1 4053518 R 83332 CDS NP_218130.1 15610749 885124 complement(4053881..4054042) 1 NC_000962.3 Rv3613c, (MTCY07H7B.09), len: 53 aa. Hypothetical unknown protein.; Hypothetical protein 4054042 885124 Rv3613c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218130.1 4053881 R 83332 CDS NP_218131.1 15610750 885777 complement(4054142..4054696) 1 NC_000962.3 Rv3614c, (MTCY07H7B.08), len: 184 aa. EspD, ESX-1 secretion-associated protein, equivalent to Q49730|ML0407|B1620_C3_264|MLCL383.03 hypothetical 24.2 KDA protein from Mycobacterium leprae (216 aa) FASTA scores: opt: 899, E(): 1.7e-51, (71.3% identity in 188 aa overlap); and similar to two hypothetical proteins from Mycobacterium leprae: Q9CDD6|ML0056 (169 aa), FASTA scores: opt: 285,E(): 1.2e-11, (38.35% identity in 172 aa overlap); and O33090|MLCB628.19c (338 aa), FASTA scores: opt: 289, E(): 1.2e-11, (38.95% identity in 172 aa overlap). Also highly similar to O69732|Rv3867|MTV027.02 hypothetical 19.9 KDA protein from Mycobacterium tuberculosis (183 aa), FASTA scores: opt: 563, E(): 1e-29, (54.9% identity in 173 aa overlap). Rv3614c and Rv3882c interact, by yeast two-hybrid analysis (See MacGurn et al., 2005). EspD|Rv3614c is still secreted by M. tuberculosis H37Rv and Erdman ESX-1 secretion system mutants, but at levels lower than in wild-type (See Chen et al., 2012).; ESX-1 secretion-associated protein EspD 4054696 espD 885777 espD Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspD NP_218131.1 4054142 R 83332 CDS NP_218132.1 15610751 885770 complement(4054812..4055123) 1 NC_000962.3 Rv3615c, (MTCY07H7B.07), len: 103 aa. EspC, ESX-1 secretion-associated protein, equivalent to Q49723|ML0406|B1620_C2_214|MLCL383 hypothetical 11.1 KDA protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 364, E(): 4.1e-18, (60.85% identity in 92 aa overlap). Also shows similarity to P96212|Rv3865|MTCY01A6.03 hypothetical 10.6 KDA protein from Mycobacterium tuberculosis (103 aa), FASTA scores: opt: 198, E(): 6.8e-07, (36.25% identity in 102 aa overlap). Has been shown to interact with itself, by yeast two-hybrid analysis (See MacGurn et al., 2005).; ESX-1 secretion-associated protein EspC 4055123 espC 885770 espC Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspC NP_218132.1 4054812 R 83332 CDS NP_218133.1 15610752 885377 complement(4055197..4056375) 1 NC_000962.3 Rv3616c, (MTCY07H7B.06), len: 392 aa. EspA, ESX-1 secretion-associated protein A. Ala-, gly-rich protein,equivalent to Q49722|ML0405|B1620_C2_213|MLCL383.01 hypothetical 40.8 KDA protein from Mycobacterium leprae (394 aa) FASTA scores: opt: 1620, E(): 5.3e-75, (62.7% identity in 394 aa overlap). Also similar to P96213|Rv3864|MTCY01A6.04c hypothetical 42.1 KDA protein from Mycobacterium tuberculosis (402 aa), FASTA scores: opt: 389, E(): 1.1e-12, (31.75% identity in 400 aa overlap).; ESX-1 secretion-associated protein A, EspA 4056375 espA 885377 espA Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein A, EspA NP_218133.1 4055197 R 83332 CDS NP_218134.1 15610753 885769 4057733..4058701 1 NC_000962.3 Rv3617, (MTCY07H7B.05c, MTCY15C10.35c), len: 322 aa. Probable ephA, epoxide hydrolase (see citation below),similar to many e.g. Q9A8W9|CC1229 from Caulobacter crescentus (330 aa), FASTA scores: opt: 965, E(): 1.8e-51,(46.15% identity in 323 aa overlap); Q9M9W5|F18C1.13 from Arabidopsis thaliana (Mouse-ear cress) (331 aa), FASTA scores: opt: 778, E(): 4.3e-40, (40.35% identity in 332 aa overlap); Q9S7P1 from Oryza sativa (Rice) (322 aa), FASTA scores: opt: 774, E(): 7.4e-40, (41.1% identity in 321 aa overlap); P80299|HYES_RAT|EPHX2 from Rattus norvegicus (Rat) (554 aa), FASTA scores: opt: 759, E(): 9.5e-39,(40.5% identity in 306 aa overlap) (similarity only with the C-terminal part for this one); etc. Similar to alpha/beta hydrolase fold. Contains PS00888 Cyclic nucleotide-binding domain signature 1.; Probable epoxide hydrolase EphA (epoxide hydratase) (arene-oxide hydratase) 4058701 ephA 885769 ephA Mycobacterium tuberculosis H37Rv Probable epoxide hydrolase EphA (epoxide hydratase) (arene-oxide hydratase) NP_218134.1 4057733 D 83332 CDS NP_218135.1 15610754 885276 4058698..4059885 1 NC_000962.3 Rv3618, (MTCY15C10.34c, MTCY07H7B.04c), len: 395 aa. Possible monooxygenase, similar to others (principally bacterial luciferases alpha chain) e.g. Q9JN87|MMYO putative alkanal monooxygenase from Streptomyces coelicolor (373 aa), FASTA scores: opt: 949, E(): 8.9e-54, (41.7% identity in 374 aa overlap); Q9EUT9|limb limonene monooxygenase from Rhodococcus erythropolis (387 aa), FASTA scores: opt: 856, E(): 9.1e-48, (42.0% identity in 388 aa overlap); AAK72698 LUXA-like protein from Bradyrhizobium japonicum (458 aa) FASTA scores: opt: 350, E(): 4.4e-15,(29.7% identity in 347 aa overlap); Q9K4C1|2SC6G5.34c putative alkanal monooxygenase (luciferase) from Streptomyces coelicolor (342 aa), FASTA scores: opt: 291,E(): 2.2e-11, (26.5% identity in 362 aa overlap); etc. Also similar to P95278|Rv1936|MTCY09F9.28c hypothetical 41.8 KDA protein from Mycobacterium tuberculosis (369 aa), FASTA scores: opt: 473, E(): 4.3e-23, (32.55% identity in 378 aa overlap).; Possible monooxygenase 4059885 885276 Rv3618 Mycobacterium tuberculosis H37Rv Possible monooxygenase NP_218135.1 4058698 D 83332 CDS NP_218136.1 15610755 885328 complement(4059984..4060268) 1 NC_000962.3 Rv3619c, (MTCY15C10.33, MTCY07H7B.03, MT3721), len: 94 aa. EsxV, ESAT-6 like protein (see citations below),highly similar to many Mycobacterial ESAT-6 like proteins e.g. O53942|ES65_MYCTU putative ESAT-6 like protein 5 from Mycobacterium tuberculosis (94 aa), FASTA scores: opt: 582,E(): 4.4e-33, (92.55% identity in 94 aa overlap); Q49946|ES6X_MYCLE|U1756D putative ESAT-6 like protein X from Mycobacterium leprae (95 aa), FASTA scores: opt: 409,E(): 2.5e-21, (64.15% identity in 92 aa overlap); etc. Strictly identical to P96364|ES61_MYCTU|Rv1037c|MT1066|MTCY10G2.12 putative ESAT-6 like protein 1 (94 aa). Belongs to the ESAT6 family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Putative ESAT-6 like protein EsxV (ESAT-6 like protein 1) 4060268 esxV 885328 esxV Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxV (ESAT-6 like protein 1) NP_218136.1 4059984 R 83332 CDS NP_218137.1 15610756 885787 complement(4060295..4060591) 1 NC_000962.3 Rv3620c, (MTCY15C10.32, MTCY07H7B.02, MT3722), len: 98 aa. EsxW, ESAT-6 like protein (see citation below). Member of the M. tuberculosis hypothetical QILSS protein family with Rv1038c, Rv1792, Rv2347c and Rv1197|O05299|ES63_MYCTU|MT1235|MTCI364.09 putative ESAT-6 like protein 3 from Mycobacterium tuberculosis (98 aa),FASTA scores: opt: 638, E(): 2.3e-36, (97.95% identity in 98 aa overlap). Also similar to Q49945|ES6Y_MYCLE putative ESAT-6 like protein Y from Mycobacterium leprae (100 aa),FASTA scores: opt: 370, E(): 2.1e-18, (57.9% identity in 95 aa overlap); etc. Belongs to the ESAT6 family.; Putative ESAT-6 like protein EsxW (ESAT-6 like protein 10) 4060591 esxW 885787 esxW Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxW (ESAT-6 like protein 10) NP_218137.1 4060295 R 83332 CDS YP_177998.1 57117135 885097 complement(4060648..4061889) 1 NC_000962.3 Rv3621c, (MTCY15C10.31, MTCY07H7B.01), len: 413 aa. PPE65, Member of the Mycobacterium tuberculosis PPE family,ala-, gly-rich proteins, similar to many e.g. Q10813|YS92_MYCTU|Rv2892c|MT2959|MTCY274.23c (408 aa) FASTA scores: opt: 955, E(): 1.8e-42, (44.45% identity in 423 aa overlap).; PPE family protein PPE65 4061889 PPE65 885097 PPE65 Mycobacterium tuberculosis H37Rv PPE family protein PPE65 YP_177998.1 4060648 R 83332 CDS YP_177999.1 57117136 885712 complement(4061899..4062198) 1 NC_000962.3 Rv3622c, (MTCY15C10.30), len: 99 aa. PE32, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), but no glycine rich C-terminus present. Similar to others e.g. O53938|Rv1788|MTV049.10 (99 aa),FASTA scores: opt: 376, E(): 7.1e-17, (65.6% identity in 96 aa overlap).; PE family protein PE32 4062198 PE32 885712 PE32 Mycobacterium tuberculosis H37Rv PE family protein PE32 YP_177999.1 4061899 R 83332 CDS NP_218140.1 15610759 885233 4062527..4063249 1 NC_000962.3 Rv3623, (MTCY15C10.29c), len: 240 aa. Probable lpqG,conserved lipoprotein, showing some similarity with hypothetical proteins e.g. Q57432 from Methanosarcina barkeri (251 aa), FASTA scores: opt: 319, E(): 6.8e-12,(31.2% identity in 218 aa overlap); Q9PEA5|XF1123 outer membrane protein from Xylella fastidiosa (242 aa) FASTA scores: opt: 312, E(): 1.7e-11, (28.25% identity in 237 aa overlap); BAB49547|MLR2408 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (236 aa), FASTA scores: opt: 304, E(): 5e-11, (27.05% identity in 244 aa overlap); etc. Has suitable signal peptide and prokaryotic membrane lipoprotein lipid attachment site (PS00013).; Probable conserved lipoprotein LpqG 4063249 lpqG 885233 lpqG Mycobacterium tuberculosis H37Rv Probable conserved lipoprotein LpqG NP_218140.1 4062527 D 83332 CDS NP_218141.1 15610760 885398 complement(4063254..4063904) 1 NC_000962.3 Rv3624c, (MTCY15C10.28), len: 216 aa. Hpt (alternate gene name: hprT), hypoxanthine-guanine phosphoribosyltransferase (but seems to have a 35 aa extension at N-terminus), equivalent to other mycobacterial hypoxanthine-guanine phosphoribosyltransferases e.g. P96794 from Mycobacterium avium (203 aa), FASTA scores: opt: 1136,E(): 1.2e-65, (88.5% identity in 200 aa overlap); and O69537|HPT|ML0214 from Mycobacterium leprae (213 aa), FASTA scores: opt: 1115, E(): 2.8e-64, (81.6% identity in 212 aa overlap). Also similar to others e.g. Q9X8I5|SCE9.12c from Streptomyces coelicolor (187 aa), FASTA scores: opt: 724,E(): 2.4e-39, (60.55% identity in 180 aa overlap); P37472|HPRT_BACSU|HPT from Bacillus subtilis (180 aa) FASTA scores: opt: 574, E(): 9.1e-30, (48.6% identity in 181 aa overlap); etc. Equivalent to AAK48087 from Mycobacterium tuberculosis strain CDC1551 (202 aa) but longer 14 aa. Contains PS00103 Purine/pyrimidine phosphoribosyltransferases signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family.; Hypoxanthine-guanine phosphoribosyltransferase Hpt (HGPRT) (HGPRTase) (hypoxanthine phosphoribosyltransferase) (imp pyrophosphorylase) (imp diphosphorylase) (transphosphoribosyltransferase) (guanine phosphoribosyltransferase) 4063904 hpt 885398 hpt Mycobacterium tuberculosis H37Rv Hypoxanthine-guanine phosphoribosyltransferase Hpt (HGPRT) (HGPRTase) (hypoxanthine phosphoribosyltransferase) (imp pyrophosphorylase) (imp diphosphorylase) (transphosphoribosyltransferase) (guanine phosphoribosyltransferase) NP_218141.1 4063254 R 83332 CDS NP_218142.1 15610761 885409 complement(4063901..4064872) 1 NC_000962.3 Rv3625c, (MT3727, MTCY15C10.27), len: 323 aa. Possible mesJ, cell cycle protein, equivalent to O69538|Y0C5_MYCLE|ML0213|MLCB2548.18c hypothetical 34.1 KDA protein from Mycobacterium leprae (323 aa) FASTA scores: opt: 1592, E(): 9e-92, (78.0% identity in 327 aa overlap). Similar to bacterial hypothetical proteins Q9X8I6|SCE9.13c from Streptomyces coelicolor (352 aa) FASTA scores: opt: 705, E(): 1.4e-36, (47.85% identity in 305 aa overlap); and Q9HXZ3|PA3638 from Pseudomonas aeruginosa (442 aa), FASTA scores: opt: 382, E(): 2e-16, (40.6% identity in 271 aa overlap). But also similar (or with similarity) to bacterial cell cycle proteins (MESJ) e.g. Q9KPX0|VC2242 MESJ protein from Vibrio cholerae (440 aa), FASTA scores: opt: 363, E(): 3e-15, (34.8% identity in 253 aa overlap); Q9RV23|DR1207 (600 aa) cell cycle protein MESJ (putative/cytosine deaminase-related protein) from Deinococcus radiodurans (600 aa), FASTA scores: opt: 310,E(): 7.6e-12, (36.6% identity in 265 aa overlap) (similar only at the N-terminal end); Q9PFJ8|XF0659 cell cycle protein from Xylella fastidiosa (437 aa), FASTA scores: opt: 301, E(): 2.1e-11, (35.05% identity in 271 aa overlap); P52097|MESJ_ECOLI|B0188 putative cell cycle protein MESJ from Escherichia coli strain K12(432 aa) FASTA scores: opt: 299, E(): 2.8e-11, (34.65% identity in 277 aa overlap); etc. Belongs to the UPF0072 (MESJ/YCF62) family.; Possible cell cycle protein MesJ 4064872 mesJ 885409 mesJ Mycobacterium tuberculosis H37Rv Possible cell cycle protein MesJ NP_218142.1 4063901 R 83332 CDS NP_218143.1 15610762 885792 complement(4064851..4065903) 1 NC_000962.3 Rv3626c, (MTCY15C10.26), len: 350 aa. Conserved hypothetical protein, similar to Q9X8I7|SCE9.14c hypothetical protein from Streptomyces coelicolor (375 aa) FASTA scores: opt: 720, E(): 2.2e-38, (41.55% identity in 361 aa overlap); and shows some similarity to Q9HPS0|VNG1497C hypothetical protein (317 aa) FASTA scores: opt: 226, E(): 4.5e-07, (29.7% identity in 347 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142).; hypothetical protein 4065903 885792 Rv3626c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218143.1 4064851 R 83332 CDS NP_218144.1 15610763 885728 complement(4065900..4067285) 1 NC_000962.3 Rv3627c, (MTCY15C10.25), len: 461 aa. Conserved ala-rich protein which may have cleavable signal peptide at N-terminal end. Equivalent to O69539|MLCB2548.20c|ML0211 hypothetical 47.2 KDA protein from Mycobacterium leprae (461 aa), FASTA scores: opt: 2295, E(): 3.5e-116, (76.2% identity in 462 aa overlap); and C-terminal end shows similarity with O05758|MLCB5.28c hypothetical 24.1 KDA protein from Mycobacterium leprae (225 aa), FASTA scores: opt: 268, E(): 1.8e-07, (32.25% identity in 220 aa overlap). Also similar (or with similarity) to various proteins (notably penicillin binding proteins) e.g. Q9X8I8|SCE9.15c hypothetical 45.9 KDA protein from Streptomyces coelicolor (459 aa) FASTA scores: opt: 707,E(): 8.3e-31, (35.75% identity in 439 aa overlap); Q9Z541|SC9B2.18c putative carboxypeptidase from Streptomyces coelicolor (451 aa), FASTA scores: opt: 450,E(): 5.3e-17, (31.75% identity in 469 aa overlap); Q9JVV4|NMA0665 putative peptidase from Neisseria meningitidis (serogroup A) (or Q9JY10|NMB1797 from serogroup B) (469 aa), FASTA scores: opt: 269, E(): 3e-07,(26.15% identity in 463 aa overlap); O85665|PBP3 penicillin binding protein 3 from Neisseria gonorrhoeae (469 aa),FASTA scores: opt: 265, E(): 4.9e-07, (31.85% identity in 201 aa overlap); P45161|PBP4_HAEIN|DACB|HI1330 penicillin-binding protein 4 precursor/peptidase (479 aa) FASTA scores: opt: 230, E(): 3.8e-05, (27.9% identity in 394 aa overlap); P24228|PBP4_ECOLI|DACB|B3182 penicillin-binding protein 4 precursor from Escherichia coli strain K12 (477 aa), FASTA scores: opt: 166, E(): 0.1,(28.2% identity in 408 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 4067285 885728 Rv3627c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218144.1 4065900 R 83332 CDS NP_218145.1 15610764 885775 4067423..4067911 1 NC_000962.3 Rv3628, (MTCY15C10.24), len: 162 aa. Ppa, inorganic pyrophosphatase (see Triccas & Gicquel 2001), identical to O69540|IPYR_MYCLEPPA|ML0210|MLCB2548.21 inorganic pyrophosphatase from Mycobacterium leprae (162 aa) FASTA scores: opt: 1018, E(): 1.3e-59, (89.5% identity in 162 aa overlap). Also highly similar to many bacterial pyrophosphatases e.g. Q9X8I9|IPYR_STRCO|PPA|SCE9.16 from Streptomyces coelicolor (163 aa), FASTA scores: opt: 773,E(): 1.3e-43, (67.5% identity in 163 aa overlap); O05545|IPYR_GLUOX|PPA from Gluconobacter oxydans (Gluconobacter suboxydans) (176 aa), FASTA scores: opt: 553, E(): 3.2e-29, (53.8% identity in 145 aa overlap); P77992|IPYR_THELI|PPA from Thermococcus litoralis (176 aa) FASTA scores: opt: 537, E(): 3.5e-28, (49.35% identity in 152 aa overlap); P50308|IPYR_SULAC|PPA from Sulfolobus acidocaldarius (173 aa), FASTA scores: opt: 518, E(): 6e-27, (45.3% identity in 159 aa overlap); etc. Belongs to the PPASE family. Cofactor: requires the presence of divalent metal cation. Magnesium confers the highest activity. Binds 4 divalent cations per subunit (by similarity).; Inorganic pyrophosphatase Ppa (pyrophosphate phospho-hydrolase) (PPASE) (inorganic diphosphatase) (diphosphate phospho-hydrolase) 4067911 ppa 885775 ppa Mycobacterium tuberculosis H37Rv Inorganic pyrophosphatase Ppa (pyrophosphate phospho-hydrolase) (PPASE) (inorganic diphosphatase) (diphosphate phospho-hydrolase) NP_218145.1 4067423 D 83332 CDS NP_218146.1 15610765 885351 complement(4067957..4069054) 1 NC_000962.3 Rv3629c, (MTCY15C10.23), len: 365 aa. Probable conserved integral membrane protein, equivalent to O69543|MLCB2548.26|ML0205 putative membrane protein from Mycobacterium leprae (356 aa), FASTA scores: opt: 1547,E(): 3e-89, (66.2% identity in 361 aa overlap). Also similar to other membrane and hypothetical proteins e.g. CAC37534|SCIF3.15c putative integral membrane protein from Streptomyces coelicolor (363 aa), FASTA scores: opt: 819,E(): 7.7e-44, (51.55% identity in 351 aa overlap); Q9CGK3|YKJK hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (339 aa) FASTA scores: opt: 683, E(): 2.2e-35, (48.3% identity in 350 aa overlap); Q9KY24|SCC8A.24c putative integral membrane protein from Streptomyces coelicolor (380 aa) FASTA scores: opt: 528, E(): 1.1e-25, (50.25% identity in 372 aa overlap); Q9RJH8|SCF73.09 putative integral membrane protein from Streptomyces coelicolor (370 aa) FASTA scores: opt: 439, E(): 3.9e-20, (50.2% identity in 384 aa overlap); Q9PE36|XF1192 integral membrane protein from Xylella fastidiosa (341 aa), FASTA scores: opt: 337, E(): 8.3e-14,(47.65% identity in 361 aa overlap); etc.; Probable conserved integral membrane protein 4069054 885351 Rv3629c Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_218146.1 4067957 R 83332 CDS NP_218147.1 15610766 885802 4069175..4070470 1 NC_000962.3 Rv3630, (MTCY15C10.22c), len: 431 aa. Probable conserved integral membrane, highly similar to P71789|YF10_MYCTU|Rv1510|MTCY277.32 hypothetical 44.3 KDA protein from Mycobacterium tuberculosis (432 aa) FASTA scores: opt: 1940, E(): 2.3e-103, (70.75% identity in 424 aa overlap). Note that N-terminal end is highly similar to AAK45825|MT1558 hypothetical 18.1 KDA protein from Mycobacterium tuberculosis strain CDC1551 (172 aa) FASTA scores: opt: 649, E(): 4.2e-30, (61.65% identity in 167 aa overlap); and C-terminal end is highly similar to AAK45826|MT1560 hypothetical 25.8 KDA protein from Mycobacterium tuberculosis strain CDC1551 (256 aa), FASTA scores: opt: 1269, E(): 2.6e-65, (76.7% identity in 253 aa overlap). Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site, so could be a protease.; Probable conserved integral membrane protein 4070470 885802 Rv3630 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_218147.1 4069175 D 83332 CDS NP_218148.1 15610767 885314 4070514..4071239 1 NC_000962.3 Rv3631, (MTCY15C10.21c), len: 241 aa. Possible transferase, more specifically a glycosyltransferase ,equivalent to O69542|MLCB2548.24c|ML0207 putative transferase (putative glycosyltransferase) from Mycobacterium leprae (239 aa) FASTA scores: opt: 1303, E(): 2.8e-72, (81.2% identity in 239 aa overlap). Also similar to many dolichyl-phosphate mannose synthases and hypothetical proteins e.g. O59263|PH1585 hypothetical 34.6 KDA protein from Pyrococcus horikoshii (313 aa), FASTA scores: opt: 472, E(): 1.2e-21, (36.65% identity in 232 aa overlap); Q9V152|PAB1971 dolichyl-phosphate mannose synthase from Pyrococcus abyssi (287 aa), FASTA scores: opt: 467, E(): 2.3e-21, (35.85% identity in 223 aa overlap); Q58619|YC22_METJA|MJ1222 hypothetical protein from Methanococcus jannaschii (243 aa), FASTA scores: opt: 400, E(): 2.4e-17, (33.35% identity in 228 aa overlap); O26474|MTH374 dolichyl-phosphate mannose synthase related protein from Methanobacterium thermoautotrophicum (291 aa) FASTA scores: opt: 354, E(): 1.7e-14, (33.5% identity in 218 aa overlap); O26239|MTH136 dolichyl-phosphate mannose synthase from Methanobacterium thermoautotrophicum (220 aa), FASTA scores: opt: 345, E(): 4.8e-14, (33.5% identity in 221 aa overlap); etc.; Possible transferase (possibly glycosyltransferase) 4071239 885314 Rv3631 Mycobacterium tuberculosis H37Rv Possible transferase (possibly glycosyltransferase) NP_218148.1 4070514 D 83332 CDS NP_218149.1 15610768 885711 4071236..4071580 1 NC_000962.3 Rv3632, (MTCY15C10.20c), len: 114 aa. Possible conserved membrane protein, equivalent to O69541|MLCB2548.23c|ML0208 hypothetical 12.9 KDA protein (putative membrane protein) from Mycobacterium leprae (113 aa), FASTA scores: opt: 594, E(): 7.1e-35, (82.0% identity in 111 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved membrane protein 4071580 885711 Rv3632 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_218149.1 4071236 D 83332 CDS NP_218150.1 15610769 885252 4071791..4072666 1 NC_000962.3 Rv3633, (MTCY15C10.19c), len: 291 aa. Conserved protein, similar to Q9X5S6|MMCH from Streptomyces lavendulae (254 aa), FASTA scores: opt: 368, E(): 3.2e-16,(35.05% identity in 194 aa overlap); Q9APW1 hypothetical 32.7 KDA protein from Pseudomonas aeruginosa (295 aa),FASTA scores: opt: 359, E(): 1.3e-15, (37.65% identity in 170 aa overlap); Q9APV4 hypothetical 34.1 KDA protein from Pseudomonas aeruginosa (309 aa), FASTA scores: opt: 316,E(): 7.6e-13, (28.65% identity in 262 aa overlap). And some similarity to Q9HGD7|FUM9 FUM9P from Gibberella moniliformis (300 aa), FASTA scores: opt: 254, E(): 6.5e-09, (29.95% identity in 157 aa overlap); and P47181|YJ9S_YEAST|YJR154W|J2240 hypothetical 39.0 KDA protein from Saccharomyces cerevisiae (Baker's yeast) (346 aa), FASTA scores: opt: 190, E(): 8.5e-05, (26.75% identity in 127 aa overlap). Also similar to P71782|YF01_MYCTU|Rv1501|MT1550|MTCY277.23 from Mycobacterium tuberculosis (273 aa), FASTA scores: opt: 286, E(): 5.5e-11, (27.5% identity in 280 aa overlap).; hypothetical protein 4072666 885252 Rv3633 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218150.1 4071791 D 83332 CDS NP_215015.2 57117137 885765 complement(4072667..4073611) 1 NC_000962.3 Rv3634c, (MTCY15C10.18), len: 314 aa. GalE1,UDP-glucose 4-epimerase (see citations below), equivalent to O69544|ML0204|RMLB2|MLCB2548.27c putative sugar dehydratase (putative sugar-nucleotide dehydratase) from Mycobacterium leprae (319 aa), FASTA scores: opt: 1798,E(): 8.2e-100, (86.4% identity in 309 aa overlap). Also similar to other UDP-glucose 4-epimerases e.g. Q9WYX9|TM0509 from Thermotoga maritima (309 aa) FASTA scores: opt: 877, E(): 4.8e-45, (45.8% identity in 308 aa overlap); Q57664|GALE_METJA|MJ0211 from Methanococcus jannaschii (305 aa), FASTA scores: opt: 792, E(): 5.4e-40,(42.05% identity in 309 aa overlap); Q9K6S7|BH3649 from Bacillus halodurans (311 aa), FASTA scores: opt: 723, E(): 7e-36, (40.5% identity in 316 aa overlap); Q9HSV1|GALE2|VNG0063G from Halobacterium sp. strain NRC-1 (328 aa), FASTA scores: opt: 597, E(): 2.3e-28, (36.35% identity in 322 aa overlap); etc. Contains short-chain alcohol dehydrogenase family signature (PS00061) but this maynot be significant. Belongs to the sugar epimerase family. Note that previously known as rmlB2, a dTDP-glucose 4,6-dehydratase (see Ma et al., 2001).; UDP-glucose 4-epimerase GalE1 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) 4073611 galE1 885765 galE1 Mycobacterium tuberculosis H37Rv UDP-glucose 4-epimerase GalE1 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) NP_215015.2 4072667 R 83332 CDS NP_218152.1 15610771 885675 4073634..4075409 1 NC_000962.3 Rv3635, (MTCY15C10.17c), len: 591 aa (start unclear). Probable conserved transmembrane protein,equivalent, but longer 25 aa, to O69545|ML0203|MLCB2548.28 putative membrane protein from Mycobacterium leprae (569 aa), FASTA scores: opt: 2933, E(): 4.6e-173, (77.0% identity in 569 aa overlap).; Probable conserved transmembrane protein 4075409 885675 Rv3635 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218152.1 4073634 D 83332 CDS NP_218153.1 15610772 885274 4075752..4076099 1 NC_000962.3 Rv3636, (MTCY15C10.16c), len: 115 aa. Possible transposase, weakly similar to others e.g. O69924|SC3C8.12 putative transposase from Streptomyces coelicolor (487 aa) FASTA scores: opt: 132, E(): 0.12, (33.05% identity in 112 aa overlap); O96916 TC1-like transposase from Anopheles gambiae (African malaria mosquito) (332 aa), FASTA scores: opt: 117, E(): 0.84, (30.75% identity in 91 aa overlap); Q9R2U5|IS466A|IS466A-ORF|TNPA|IS469|SCP1.276 transposase (insertion element IS466S transposase) from Streptomyces coelicolor (513 aa), FASTA scores: opt: 114, E(): 2, (30.5% identity in 82 aa overlap); etc. Similar in part to P96288|Rv2943|MTCY24G1.06c hypothetical 45.8 KDA protein from Mycobacterium tuberculosis (413 aa), FASTA scores: opt: 533, E(): 1.4e-28, (74.55% identity in 110 aa overlap). Contains possible helix-turn-helix motif from aa 19-40 (+4.98 SD).; Possible transposase 4076099 885274 Rv3636 Mycobacterium tuberculosis H37Rv Possible transposase NP_218153.1 4075752 D 83332 CDS NP_218154.1 15610773 885496 4076484..4076984 1 NC_000962.3 Rv3637, (MTCY15C10.15c), len: 166 aa. Possible transposase. C-terminal end highly similar to Q9RLQ9|ISTA putative transposase a (fragment) from Mycobacterium bovis (102 aa), FASTA scores: opt: 397, E(): 1.4e-19, (58.8% identity in 102 aa overlap). Weakly similar to others e.g. Q9KJ02 putative transposase (fragment) from Polyangium cellulosum (329 aa), FASTA scores: opt: 191, E(): 1.6e-05,(32.1% identity in 134 aa overlap); Q9LCU2|ISTA cointegrase from Pseudomonas aeruginosa (382 aa) FASTA scores: opt: 144, E(): 0.024, (26.8% identity in 123 aa overlap); P15025|ISTA_PSEAE transposase for insertion sequence element IS21 from Pseudomonas aeruginosa (390 aa), FASTA scores: opt: 144, E(): 0.025, (26.85% identity in 123 aa overlap); etc. Also highly similar to C-terminal end of P96288|Rv2943|MTCY24G1.06c hypothetical 45.8 KDA protein from Mycobacterium tuberculosis (413 aa) FASTA scores: opt: 722, E(): 1.5e-40, (63.7% identity in 168 aa overlap).; Possible transposase 4076984 885496 Rv3637 Mycobacterium tuberculosis H37Rv Possible transposase NP_218154.1 4076484 D 83332 CDS NP_218155.1 15610774 885803 4076984..4077730 1 NC_000962.3 Rv3638, (MTCY15C10.14c), len: 248 aa. Possible transposase, highly similar to Q9RLQ8|ISTB ISTB protein from Mycobacterium bovis (266 aa), FASTA scores: opt: 784,E(): 4e-46, (78.0% identity in 259 aa overlap); and similar to others e.g. P15026|ISTB_PSEAE insertion sequence IS21 putative ATP-binding protein from Pseudomonas aeruginosa (265 aa), FASTA scores: opt: 420, E(): 2.2e-21, (38.8% identity in 255 aa overlap); Q45619|ISTB_BACST insertion sequence IS5376 putative ATP-binding protein from Bacillus stearothermophilus (251 aa), FASTA scores: opt: 402, E(): 3.6e-20, (34.5% identity in 232 aa overlap); P15026|ISTB_ECOLI ISTB protein from Escherichia coli (265 aa), FASTA scores: opt: 419, E(): 8e-23, (38.8% identity in 255 aa overlap); etc. C-terminus highly similar to C-terminus of P96287|Rv2944|MTCY24G1.05 hypothetical 25.5 KDA protein from Mycobacterium tuberculosis strain H37Rv (alias AAK47343|MT3016 IS1533, ORFB from Mycobacterium tuberculosis strain CDC1551) (238 aa), FASTA scores: opt: 784, E(): 3.6e-46, (87.4% identity in 135 aa overlap).; Possible transposase 4077730 885803 Rv3638 Mycobacterium tuberculosis H37Rv Possible transposase NP_218155.1 4076984 D 83332 CDS NP_218156.1 15610775 885244 complement(4077884..4078450) 1 NC_000962.3 Rv3639c, (MTCY15C10.13), len: 188 aa. Hypothetical protein, with C-terminus highly similar to N-terminus of P95044|Rv0698|MTCY210.15 hypothetical 22.3 KDA protein from Mycobacterium tuberculosis (203 aa), FASTA scores: opt: 224, E(): 4.5e-07, (54.8% identity in 73 aa overlap).; hypothetical protein 4078450 885244 Rv3639c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218156.1 4077884 R 83332 CDS NP_218157.1 15610776 885324 complement(4078520..4079749) 1 NC_000962.3 Rv3640c, (MTCY15C10.12), len: 409 aa. Probable transposase, highly similar to others e.g. Q48882 transposase from Mycobacterium avium (411 aa) FASTA scores: opt: 1574, E(): 6.2e-93, (59.75% identity in 400 aa overlap); Q9AKV5 putative transposase (fragment) from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 1566, E(): 1.9e-92, (60.0% identity in 395 aa overlap); Q48368 transposase from Mycobacterium avium (410 aa), FASTA scores: opt: 1561, E(): 4.1e-92, (59.4% identity in 404 aa overlap); etc.; Probable transposase 4079749 885324 Rv3640c Mycobacterium tuberculosis H37Rv Probable transposase NP_218157.1 4078520 R 83332 CDS NP_218158.1 15610777 885540 complement(4079925..4080560) 1 NC_000962.3 Rv3641c, (MTCY15C10.11), len: 211 aa. Possible fic,cell filamentation protein, similar to others e.g. Q9PCU8|XF1657 cell filamentation protein from Xylella fastidiosa (203 aa), FASTA scores: opt: 324, E(): 2.2e-14,(32.8% identity in 189 aa overlap); P20605|FIC_ECOLI|B3361 from Escherichia coli strain K12 (200 aa), FASTA scores: opt: 323, E(): 2.5e-14, (31.0% identity in 187 aa overlap); P20751|FIC_SALTY from Salmonella typhimurium (200 aa),FASTA scores: opt: 322, E(): 2.9e-14, (32.65% identity in 193 aa overlap); etc.; Possible cell filamentation protein Fic 4080560 fic 885540 fic Mycobacterium tuberculosis H37Rv Possible cell filamentation protein Fic NP_218158.1 4079925 R 83332 CDS NP_218159.1 15610778 885220 complement(4080571..4080765) 1 NC_000962.3 Rv3642c, (MTCY15C10.10), len: 64 aa. Hypothetical unknown protein.; Hypothetical protein 4080765 885220 Rv3642c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218159.1 4080571 R 83332 CDS NP_218160.1 15610779 885600 4081160..4081351 1 NC_000962.3 Rv3643, (MTCY15C10.09c), len: 63 aa (questionable ORF). Identical to AAK48106 from Mycobacterium tuberculosis strain CDC1551 (33 aa) but longer 30 aa.; Hypothetical protein 4081351 885600 Rv3643 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218160.1 4081160 D 83332 CDS NP_218161.1 15610780 885553 complement(4081516..4082721) 1 NC_000962.3 Rv3644c, (MTCY15C10.08), len: 401 aa. Possible DNA polymerase, equivalent to O69546|MLCB2548.29c|ML0202 hypothetical 42.7 KDA protein from Mycobacterium leprae (405 aa), FASTA scores: opt: 2180, E(): 6.1e-116, (84.4% identity in 404 aa overlap). Similar (in totality or in first 200 aa) to DNA polymerases III, delta' or gamma subunit, e.g. Q9X906|SCH5.03c putative DNA polymerase from Streptomyces coelicolor (401 aa), FASTA scores: opt: 1022,E(): 1.5e-50, (47.05% identity in 404 aa overlap); Q9RRS5|DR2410 DNA polymerase III, tau/gamma subunit from Deinococcus radiodurans (615 aa), FASTA scores: opt: 370,E(): 1.3e-13, (29.95% identity in 394 aa overlap); P28631|HOLB_ECOLI|B1099 DNA polymerase III, delta' subunit from Escherichia coli strain K12 (334 aa), FASTA scores: opt: 345, E(): 2.2e-12, (33.45% identity in 239 aa overlap); Q9JTS1|DNAZX|NMA1656 DNA polymerase III tau and gamma chains from Neisseria meningitidis (serogroup A) (709 aa), FASTA scores: opt: 346, E(): 3.3e-12, (28.55% identity in 364 aa overlap); etc.; Possible DNA polymerase 4082721 885553 Rv3644c Mycobacterium tuberculosis H37Rv Possible DNA polymerase NP_218161.1 4081516 R 83332 CDS NP_218162.1 15610781 885620 4082807..4084456 1 NC_000962.3 Rv3645, (MTCY15C10.07c), len: 549 aa. Probable conserved transmembrane protein, equivalent, but longer 20 aa, to O69547|ML0201|MLCB2548.30 putative membrane protein from Mycobacterium leprae (530 aa), FASTA scores: opt: 2958, E(): 1.5e-168, (85.5% identity in 530 aa overlap). Also closely related to several other hypothetical M. tuberculosis proteins, e.g. Q10631|YD18_MYCTU|Rv1318c|MT1359|MTCY130.03c (541 aa) FASTA scores: opt: 1105, E(): 2.7e-58, (39.35% identity in 506 aa overlap); Q10633|YD20_MYCTU|Rv1320c|MT1362|MTCY130.05c (567 aa) FASTA scores: opt: 1031, E(): 7.1e-54, (38.1% identity in 509 aa overlap); Q10632|YD19_MYCTU|Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 1016, E(): 5.3e-53, (37.1% identity in 531 aa overlap); etc. Also similar at C-terminal end to many adenylate cyclases e.g. O83498|TP0485 from Treponema pallidum (614 aa) FASTA scores: opt: 365, E(): 3.2e-14, (31.55% identity in 317 aa overlap); P94180|CYAA from Anabaena sp. strain PCC 7120 (735 aa), FASTA scores: opt: 364, E(): 4.2e-14, (32.75% identity in 229 aa overlap); etc.; Probable conserved transmembrane protein 4084456 885620 Rv3645 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218162.1 4082807 D 83332 CDS NP_218163.1 15610782 885608 complement(4084453..4087257) 1 NC_000962.3 Rv3646c, (MTCY15C10.06), len: 934 aa. TopA, DNA topoisomerase I (see citations below), equivalent to O69548|TOP1_MYCLE|TOPA|ML0200|MLCB2548.31c DNA topoisomerase I from Mycobacterium leprae (947 aa) FASTA scores: opt: 5150, E(): 0, (84.6% identity in 936 aa overlap). Also highly similar to many e.g. Q9X909|TOP1_STRCO|TOPA|SCH5.06c from Streptomyces coelicolor (952 aa), FASTA scores: opt: 2754, E(): 1.3e-153, (61.3% identity in 928 aa overlap); P73810|TOP1_SYNY3|TOPA|SLR2058 from Synechocystis sp. strain PCC 6803 (898 aa), FASTA scores: opt: 1442, E(): 9.1e-77, (47.15% identity in 927 aa overlap); P47368|TOP1_MYCGE|TOPA|MG122 from Mycoplasma genitalium (709 aa), FASTA scores: opt: 865, E(): 4.8e-43, (30.3% identity in 736 aa overlap); P06612|TOP1_ECOLI|TOPA|SUPX|B1274 from Escherichia coli strain K12 (865 aa), FASTA scores: opt: 397, E(): 0, (39.6% identity in 704 aa overlap); etc. Belongs to prokaryotic type I/III topoisomerase family.; DNA topoisomerase I TopA (omega-protein) (relaxing enzyme) (untwisting enzyme) (swivelase) (type I DNA topoisomerase) (nicking-closing enzyme) (TOPO I) 4087257 topA 885608 topA Mycobacterium tuberculosis H37Rv DNA topoisomerase I TopA (omega-protein) (relaxing enzyme) (untwisting enzyme) (swivelase) (type I DNA topoisomerase) (nicking-closing enzyme) (TOPO I) NP_218163.1 4084453 R 83332 CDS NP_218164.1 15610783 885217 complement(4087610..4088188) 1 NC_000962.3 Rv3647c, (MTCY15C10.05), len: 192 aa. Conserved hpothetical protein, equivalent to O69549|MLCB2548.32c|ML0199 conserved hypothetical protein from Mycobacterium leprae (200 aa), FASTA scores: opt: 1029, E(): 9e-58, (80.4% identity in 199 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 4088188 885217 Rv3647c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218164.1 4087610 R 83332 CDS NP_218165.1 15610784 885837 complement(4088328..4088531) 1 NC_000962.3 Rv3648c, (MTCY15C10.04), len: 67 aa. Probable cspA,cold shock protein A, identical to O69550|CSPB|CSPA|ML0198 small cold-shock protein from Mycobacterium leprae (67 aa) FASTA scores: opt: 451, E(): 3.7e-27, (97.0% identity in 67 aa overlap). Also highly similar to many e.g. Q9KGW0|CSPA from Mycobacterium smegmatis (67 aa) FASTA scores: opt: 439, E(): 2.9e-26, (92.55% identity in 67 aa overlap); P54584|CSP_ARTGO from Arthrobacter globiformis (67 aa),FASTA scores: opt: 335, E(): 1.5e-18, (73.45% identity in 64 aa overlap); O30875|CSPA_MICLU from Micrococcus luteus (Micrococcus lysodeikticus); Q9Z5R4|CSPA_BORPE from Bordetella pertussis (67 aa) FASTA scores: opt: 294, E(): 1.7e-15, (59.7% identity in 67 aa overlap); etc. Contains 'cold-shock' DNA-binding domain signature (PS00352) at N-terminal end. Belongs to the cold-shock domain (CSD) family.; Probable cold shock protein A CspA 4088531 cspA 885837 cspA Mycobacterium tuberculosis H37Rv Probable cold shock protein A CspA NP_218165.1 4088328 R 83332 CDS NP_218166.1 15610785 885841 4088781..4091096 1 NC_000962.3 Rv3649, (MTCY15C10.03c), len: 771 aa. Probable helicase, similar to many (known or hypothetical) ATP-dependent helicases e.g. Q9X915|SCH5.13 putative helicase from Streptomyces coelicolor (815 aa) FASTA scores: opt: 2550, E(): 9.6e-139, (52.45% identity in 774 aa overlap); Q05549|YDR291W|D9819.1 protein similar to several DNA helicases from Saccharomyces cerevisiae (Baker's yeast) (1077 aa), FASTA scores: opt: 1161, E(): 5.9e-59, (31.05% identity in 780 aa overlap); P50830|YPRA_BACSU hypothetical helicase from Bacillus subtilis (749 aa), FASTA scores: opt: 1154, E(): 1.1e-58,(34.05% identity in 734 aa overlap); Q9KC10|BH1764 ATP-dependent RNA helicase from Bacillus halodurans (764 aa), FASTA scores: opt: 1122, E(): 8e-57, (32.3% identity in 759 aa overlap); etc. Seems similar to dead/DEAH box helicase family, and to helicase C-terminal domain.; Probable helicase 4091096 885841 Rv3649 Mycobacterium tuberculosis H37Rv Probable helicase NP_218166.1 4088781 D 83332 CDS YP_178000.1 57117138 885832 4091233..4091517 1 NC_000962.3 Rv3650, (MTCY15C10.02c), len: 94 aa. PE33, Short protein, member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), but without the repetitive gly-rich region, similar to the N-terminal part of many e.g. O53809|Rv0746|MTV041.20 PGRS-family protein (783 aa),FASTA scores: opt: 363, E(): 2.1e-15, (76.55% identity in 81 aa overlap).; PE family protein PE33 4091517 PE33 885832 PE33 Mycobacterium tuberculosis H37Rv PE family protein PE33 YP_178000.1 4091233 D 83332 CDS NP_218168.1 15610787 885296 4091841..4092878 1 NC_000962.3 Rv3651, (MTCY15C10.01c), len: 345 aa. Hypothetical protein, with some similarity to Q9ZHK1 hypothetical 36.5 KDA protein from Rhodococcus sp. X309 (329 aa) FASTA scores: opt: 332, E(): 3.4e-13, (27.4% identity in 321 aa overlap).; hypothetical protein 4092878 885296 Rv3651 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218168.1 4091841 D 83332 CDS YP_178001.1 57117139 886260 4093632..4093946 1 NC_000962.3 Rv3652, (MTV025.001A), len: 104 aa. PE_PGRS60,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu,2002), similar at N-terminal end with many e.g. P56877|Y278_MYCTU|Rv0278c|MTV035.06c (957 aa) FASTA scores: opt: 242, E(): 3e-09, (77.35% identity in 53 aa overlap). Originally annotated as the first part of a PE-PGRS family protein (Rv3653/PE_PGRS61 being the second part) but more similar to a PE family protein. Length extended since first submission (+50 aa).; PE-PGRS family-related protein PE_PGRS60 4093946 PE_PGRS60 886260 PE_PGRS60 Mycobacterium tuberculosis H37Rv PE-PGRS family-related protein PE_PGRS60 YP_178001.1 4093632 D 83332 CDS YP_178002.1 57117140 886259 4093940..4094527 1 NC_000962.3 Rv3653, (MTV025.001B), len: 195 aa. PE_PGRS61,Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu,2002), highly similar to the C-termini of members of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, e.g. MTCY1A11_25, MTCY28_25, MTCY130_10,MTCY1A10_19, MTCY21B4_13, MTCI418B_6,MTCY28_34, MTV004_1,MTCY441_4; etc. Originally annotated as the second part of a PE-PGRS family protein (Rv3652/PE_PGRS60 being the first part). Start shortened since first submission (-50 aa).; PE-PGRS family-related protein PE_PGRS61 4094527 PE_PGRS61 886259 PE_PGRS61 Mycobacterium tuberculosis H37Rv PE-PGRS family-related protein PE_PGRS61 YP_178002.1 4093940 D 83332 CDS NP_218171.1 15610790 885612 complement(4094660..4094914) 1 NC_000962.3 Rv3654c, (MTV025.002c), len: 84 aa. Hypothetical protein, similar to C-terminus of Q9X916|SCH5.14c membrane spanning protein from Streptomyces coelicolor (230 aa) FASTA scores: opt: 176, E(): 2.4e-05, (47.0% identity in 83 aa overlap). Equivalent to AAK48118 from Mycobacterium tuberculosis strain CDC1551 but shorter 18 aa.; hypothetical protein 4094914 885612 Rv3654c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218171.1 4094660 R 83332 CDS NP_218172.1 15610791 885614 complement(4094923..4095300) 1 NC_000962.3 Rv3655c, (MTV025.003c), len: 125 aa. Hypothetical protein, with similarity to Q9X917|SCH5.15c hypothetical 15.2 KDA protein from Streptomyces coelicolor (150 aa) FASTA scores: opt: 211, E(): 7.7e-07, (39.65% identity in 111 aa overlap). Equivalent to AAK48119 from Mycobacterium tuberculosis strain CDC1551 (99 aa) but longer 26 aa at the C-terminus.; hypothetical protein 4095300 885614 Rv3655c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218172.1 4094923 R 83332 CDS NP_218173.1 15610792 885624 complement(4095324..4095530) 1 NC_000962.3 Rv3656c, (MTV025.004c), len: 68 aa. Conserved hypothetical protein, similar to Q9X918|SCH5.16c small hypothetical protein from Streptomyces coelicolor (75 aa),FASTA scores: opt: 129, E(): 0.0039, (40.0% identity in 60 aa overlap). Equivalent to AAK48120 from Mycobacterium tuberculosis strain CDC1551 (42 aa) but longer 26 aa.; hypothetical protein 4095530 885624 Rv3656c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218173.1 4095324 R 83332 CDS NP_218174.1 15610793 885619 complement(4095540..4096115) 1 NC_000962.3 Rv3657c, (MTV025.005c), len: 191 aa. Possible conserved membrane protein, rich in ala residues, similar to Q9X919|SCH5.17c putative integral membrane protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 324,E(): 4.7e-12, (40.9% identity in 154 aa overlap).; Possible conserved alanine rich membrane protein 4096115 885619 Rv3657c Mycobacterium tuberculosis H37Rv Possible conserved alanine rich membrane protein NP_218174.1 4095540 R 83332 CDS NP_218175.1 15610794 885618 complement(4096139..4096939) 1 NC_000962.3 Rv3658c, (MTV025.006c), len: 266 aa. Probable conserved transmembrane protein, similar to Q9X920|SCH5.18c putative integral membrane protein from Streptomyces coelicolor (321 aa), FASTA scores: opt: 335, E(): 4.1e-13,(38.05% identity in 247 aa overlap).; Probable conserved transmembrane protein 4096939 885618 Rv3658c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218175.1 4096139 R 83332 CDS YP_178003.1 57117141 885627 complement(4096936..4097994) 1 NC_000962.3 Rv3659c, (MTV025.007c), len: 352 aa. Conserved hypothetical protein, highly similar, but always shorter (various lengths) at N-terminus, to Q9X921|SCH5.19c putative secretory protein from Streptomyces coelicolor (523 aa), FASTA scores: opt: 1287, E(): 5.3e-66, (59.85% identity in 351 aa overlap); Q9HW98|PA4302 probable type II secretion system protein from Pseudomonas aeruginosa (421 aa), FASTA scores: opt: 776, E(): 5.4e-37, (42.8% identity in 320 aa overlap); AAK65510|CPAF2 probable CPAF2 PILUS assembly protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (497 aa) FASTA scores: opt: 769,E(): 1.5e-36, (40.45% identity in 309 aa overlap); Q9KY93|SCK15.11 putative secretory protein from Streptomyces coelicolor (445 aa), FASTA scores: opt: 751,E(): 1.5e-35, (38.15% identity in 333 aa overlap); etc. Contains PS00017 ATP/GTP binding site motif A (P-loop). Note that previously known as trbB.; hypothetical protein 4097994 885627 Rv3659c Mycobacterium tuberculosis H37Rv hypothetical protein YP_178003.1 4096936 R 83332 CDS NP_218177.1 15610796 885319 complement(4098096..4099148) 1 NC_000962.3 Rv3660c, (MTV025.008c), len: 350 aa. Conserved hypothetical protein, similar to O33612 protein concerned in inhibition of morphological differentiation in Streptomyces azureus from Streptomyces cyaneus (Streptomyces curacoi) (370 aa), FASTA scores: opt: 655,E(): 5.9e-31, (42.2% identity in 315 aa overlap); Q9X922|SCH5.20c putative septum site determining protein from Streptomyces coelicolor (396 aa), FASTA scores: opt: 592, E(): 2.9e-27, (43.25% identity in 275 aa overlap). And shows some similarity to AAK65513|CPAE2 probable CPAE2 PILUS assembly protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (586 aa) FASTA scores: opt: 212, E(): 5.1e-05, (25.75% identity in 295 aa overlap); and several cell division inhibitors or septum site-determining proteins. Equivalent to AAK48124 from Mycobacterium tuberculosis strain CDC1551 (261 aa) but longer 89 aa.; hypothetical protein 4099148 885319 Rv3660c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218177.1 4098096 R 83332 CDS NP_218178.1 15610797 885316 4099647..4100510 1 NC_000962.3 Rv3661, (MTV025.009), len: 287 aa. Conserved hypothetical protein, highly similar to O33611|IMD_STRCN from Streptomyces cyaneus (Streptomyces curacoi) protein involved in inhibition of morphological differentiation in Streptomyces azureus (belongs to the SerB family) (277 aa) FASTA scores: opt: 1073, E(): 3.5e-61, (61.45% identity in 262 aa overlap); and Q9X923|SCH5.21 putative morphological differentiation-associated protein from Streptomyces coelicolor (268 aa), FASTA scores: opt: 1057, E(): 3.6e-60,(61.45% identity in 262 aa overlap). Also similar to various bacterial proteins (principally serB-related proteins) e.g. Q49823|ML2424 hypothetical SERB protein from Mycobacterium leprae (300 aa), FASTA scores: opt: 452, E(): 1.4e-21, (35.8% identity in 257 aa overlap); Q9WX12|SCE68.20 hypothetical 32.0 KDA protein from Streptomyces coelicolor (298 aa), FASTA scores: opt: 415,E(): 3.1e-19, (33.55% identity in 280 aa overlap); Q9RIT2|SERB phosphoserine phosphatase (fragment) from Streptomyces coelicolor (266 aa), FASTA scores: opt: 405,E(): 1.2e-18, (34.1% identity in 261 aa overlap); etc. Also similar to Q11169|Y505_MYCTU|Rv0505c|MTCY20G9.32c hypothetical 39.5 KDA protein from Mycobacterium tuberculosis (373 aa), FASTA scores: opt: 454, E(): 1.2e-21, (35.15% identity in 276 aa overlap). Belongs to the SerB family.; hypothetical protein 4100510 885316 Rv3661 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218178.1 4099647 D 83332 CDS NP_218179.1 15610798 885178 complement(4101265..4102035) 1 NC_000962.3 Rv3662c, (MTV025.010c), len: 256 aa. Conserved hypothetical protein, equivalent to Q9CB99|ML2289 hypothetical protein from Mycobacterium leprae (256 aa) FASTA scores: opt: 1255, E(): 3.3e-69, (78.05% identity in 255 aa overlap). Also similar to Q9X924|SCH5.22c putative oxidoreductase from Streptomyces coelicolor (274 aa), FASTA scores: opt: 289, E(): 1.8e-10, (39.25% identity in 270 aa overlap).; hypothetical protein 4102035 885178 Rv3662c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218179.1 4101265 R 83332 CDS NP_218180.1 15610799 885039 complement(4102032..4103678) 1 NC_000962.3 Rv3663c, (MTV025.011c), len: 548 aa. Probable dppD,dipeptide-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins e.g. AAK65441|SMA1434 probable ABC transporter ATP-binding protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (550 aa), FASTA scores: opt: 1528, E(): 1e-78, (46.25% identity in 545 aa overlap); O50270|MOAD MOAD protein from Agrobacterium radiobacter (588 aa), FASTA scores: opt: 1354, E(): 6.7e-69, (42.9% identity in 541 aa overlap); Q9KM01|VCA0588 putative peptide ABC transporter ATP-binding protein from Vibrio cholerae (530 aa), FASTA scores: opt: 951, E(): 3.1e-46, (44.0% identity in 534 aa overlap); BAB49448|MLR2279 ATP-binding protein of peptide ABC transporter from Rhizobium loti (Mesorhizobium loti) (604 aa), FASTA scores: opt: 949, E(): 4.4e-46, (41.55% identity in 544 aa overlap); etc. Contains 2 PS00211 ABC transporters family signature, and 2 PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable dipeptide-transport ATP-binding protein ABC transporter DppD 4103678 dppD 885039 dppD Mycobacterium tuberculosis H37Rv Probable dipeptide-transport ATP-binding protein ABC transporter DppD NP_218180.1 4102032 R 83332 CDS NP_218181.1 15610800 885483 complement(4103675..4104475) 1 NC_000962.3 Rv3664c, (MTV025.012c), len: 266 aa. Probable dppC,dipeptide-transport integral membrane protein ABC-transporter (see Braibant et al., 2000), similar to many peptide permeases e.g. Q9F351|SC9E12.04 putative peptide transport system integral membrane from Streptomyces coelicolor (305 aa), FASTA scores: opt: 901,E(): 1.1e-47, (51.15% identity in 262 aa overlap); Q9KFX1|APPC|BH0349 oligopeptide ABC transporter (permease) from Bacillus halodurans (305 aa), FASTA scores: opt: 652,E(): 1.5e-32, (35.55% identity in 270 aa overlap); P94312|DPPC_BACFI dipeptide transport system permease protein from Bacillus firmus (304 aa), FASTA scores: opt: 642, E(): 5.9e-32, (35.75% identity in 263 aa overlap); P24139|OPPC_BACSU|SPO0KC oligopeptide transport system permease protein from Bacillus subtilis (305 aa), FASTA scores: opt: 637, E(): 1.2e-31, (37.4% identity in 262 aa overlap); P26904|DPPC_BACSU|DCIAC dipeptide transport system permease protein from Bacillus subtilis (320 aa),FASTA scores: opt: 621, E(): 1.2e-30, (39.9% identity in 263 aa overlap); etc. Has similarity with integral membrane components of other binding-protein-dependent transport systems. Belongs to the OPPBC subfamily.; Probable dipeptide-transport integral membrane protein ABC transporter DppC 4104475 dppC 885483 dppC Mycobacterium tuberculosis H37Rv Probable dipeptide-transport integral membrane protein ABC transporter DppC NP_218181.1 4103675 R 83332 CDS NP_218182.1 15610801 885474 complement(4104531..4105457) 1 NC_000962.3 Rv3665c, (MTV025.013c), len: 308 aa. Probable dppB,dipeptide-transport integral membrane protein ABC-transporter (see citation below), similar to many peptide permeases e.g. Q9F352|SC9E12.03 putative peptide transport system integral membrane protein from Streptomyces coelicolor (307 aa), FASTA scores: opt: 1145,E(): 1.8e-61, (57.65% identity in 307 aa overlap); Q53191|Y4TP_RHISN probable peptide ABC transporter permease protein Rhizobium sp. strain NGR234 (313 aa), FASTA scores: opt: 653, E(): 5.2e-32, (31.2% identity in 314 aa overlap); P24138|OPPB_BACSU oligopeptide transport system permease from Bacillus subtilis (311 aa), FASTA scores: opt: 643,E(): 2.1e-31, (33.45% identity in 305 aa overlap); etc. Belongs to the OPPBC subfamily.; Probable dipeptide-transport integral membrane protein ABC transporter DppB 4105457 dppB 885474 dppB Mycobacterium tuberculosis H37Rv Probable dipeptide-transport integral membrane protein ABC transporter DppB NP_218182.1 4104531 R 83332 CDS NP_218183.1 15610802 885315 complement(4105459..4107084) 1 NC_000962.3 Rv3666c, (MTV025.014c), len: 541 aa. Probable dppA,dipeptide-binding lipoprotein component of dipeptide transport system (see citation below), similar to many substrate-binding proteins e.g. Q9F353|SC9E12.02 putative peptide transport system secreted peptide-binding protein from Streptomyces coelicolor (544 aa), FASTA scores: opt: 1200, E(): 9e-67, (39.2% identity in 538 aa overlap); P24141|OPPA_BACSU oligopeptide-binding protein from Bacillus subtilis (545 aa), FASTA scores: opt: 523, E(): 7.9e-25, (26.15% identity in 516 aa overlap); P23843|OPPA_ECOLI periplasmic oligopeptide-binding protein from Escherichia coli (543 aa), FASTA scores: opt: 452,E(): 2e-20, (25.9% identity in 529 aa overlap); etc. Contains probable N-terminal signal sequence.; Probable periplasmic dipeptide-binding lipoprotein DppA 4107084 dppA 885315 dppA Mycobacterium tuberculosis H37Rv Probable periplasmic dipeptide-binding lipoprotein DppA NP_218183.1 4105459 R 83332 CDS NP_218184.1 15610803 885479 4107792..4109747 1 NC_000962.3 Rv3667, (MTV025.015), len: 651 aa. Probable acs,acetyl-coenzyme-a synthetase, similar to many e.g. Q9X928|SCH5.26 from Streptomyces coelicolor (651 aa) FASTA scores: opt: 2850, E(): 1.9e-164, (66.05% identity in 639 aa overlap); Q55404|ACSA_SYNY3|ACS|SLL0542 from Synechocystis sp. strain PCC 6803 (653 aa), FASTA scores: opt: 2342, E(): 8.8e-134, (55.15% identity in 649 aa overlap); P31638|ACSA_ALCEU|ACOE from Alcaligenes eutrophus (Ralstonia eutropha) (660 aa), FASTA scores: opt: 2181,E(): 4.6e-124, (52.05% identity in 665 aa overlap); P27550|ACSA_ECOLI|ACS|B4069 from Escherichia coli strain K12 (652 aa), FASTA scores: opt: 1625, E(): 0, (48.3% identity in 646 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.; Acetyl-coenzyme A synthetase Acs (acetate--CoA ligase) (acetyl-CoA synthetase) (acetyl-CoA synthase) (acyl-activating enzyme) (acetate thiokinase) (acetyl-activating enzyme) (acetate--coenzyme A ligase) (acetyl-coenzyme A synthase) 4109747 acs 885479 acs Mycobacterium tuberculosis H37Rv Acetyl-coenzyme A synthetase Acs (acetate--CoA ligase) (acetyl-CoA synthetase) (acetyl-CoA synthase) (acyl-activating enzyme) (acetate thiokinase) (acetyl-activating enzyme) (acetate--coenzyme A ligase) (acetyl-coenzyme A synthase) NP_218184.1 4107792 D 83332 CDS NP_218185.1 15610804 885609 complement(4109783..4110481) 1 NC_000962.3 Rv3668c, (MTV025.016c), len: 232 aa. Possible protease (and more specifically a putative alkaline serine protease, equivalent to Q9CB98|ML2295 hypothetical protein from Mycobacterium leprae (234 aa), FASTA scores: opt: 1249, E(): 7.4e-66, (77.5% identity in 231 aa overlap). Also similar at C-terminal end with many proteases e.g. O86984 alkaline serine protease precursor from Thermomonospora fusca (368 aa), FASTA scores: opt: 190,E(): 0.00056, (28.9% identity in 173 aa overlap); Q55353|SAPII alkaline serine protease II from Streptomyces sp (382 aa), FASTA scores: opt: 160, E(): 0.032, (27.15% identity in 199 aa overlap); O54109|SC10A5.18 putative secreted protease from Streptomyces coelicolor (411 aa),FASTA scores: opt: 155, E(): 0.066, (26.4% identity in 163 aa overlap); Q54392|SAL|SCI11.35C serine protease SAL precursor (300 aa), FASTA scores: opt: 153, E(): 0.068,(28.1% identity in 185 aa overlap); P00778|PRLA_LYSEN|alpha-LP alpha-LYTIC protease precursor (397 aa), FASTA scores: opt: 154, E(): 0.074, (26.75% identity in 172 aa overlap); etc. Also similar with Q50618|YI15_MYCTU|Rv1815|MT1863|MTCY1A11.28c hypothetical 22.8 KDA protein from Mycobacterium tuberculosis (221 aa),FASTA scores: opt: 134, E(): 0.69, (30.95% identity in 181 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Possible protease 4110481 885609 Rv3668c Mycobacterium tuberculosis H37Rv Possible protease NP_218185.1 4109783 R 83332 CDS NP_218186.1 15610805 885626 4110827..4111345 1 NC_000962.3 Rv3669, (MTV025.017), len: 172 aa. Probable conserved transmembrane protein, equivalent to Q9CB97|ML2296 putative membrane protein from Mycobacterium leprae (181 aa), FASTA scores: opt: 863, E(): 1.4e-47,(77.35% identity in 181 aa overlap). Also similar to two putative integral membrane transport proteins from Streptomyces coelicolor; Q9X930|SCH5.28 (162 aa) FASTA scores: opt: 265, E(): 6.3e-10, (37.4% identity in 155 aa overlap); and Q9X9W1|SCI7.29c (165 aa), FASTA scores: opt: 194, E(): 1.9e-05, (30.6% identity in 134 aa overlap). Contains two hydrophobic stretches in centre.; Probable conserved transmembrane protein 4111345 885626 Rv3669 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218186.1 4110827 D 83332 CDS NP_218187.1 15610806 885577 4111346..4112329 1 NC_000962.3 Rv3670, (MTV025.018), len: 327 aa. Possible ephE,epoxide hydrolase (see citation below), equivalent to Q9CB96|ML2297 putative hydrolase from Mycobacterium leprae (324 aa), FASTA scores: opt: 1799, E(): 7.2e-105, (80.55% identity in 324 aa overlap). Also similar to many hydrolases (epoxide hydrolases) and hypothetical proteins e.g. Q9X931|SCH5.29 putative hydrolase from Streptomyces coelicolor (324 aa), FASTA scores: opt: 687, E(): 1.4e-35,(40.65% identity in 327 aa overlap); Q9RRE3|DR2549 epoxide hydrolase-related protein from Deinococcus radiodurans (278 aa), FASTA scores: opt: 321, E(): 8.2e-13, (32.15% identity in 311 aa overlap); Q9K3Q1|2SCG4.13 putative hydrolase from Streptomyces coelicolor (292 aa), FASTA scores: opt: 295,E(): 3.5e-11, (30.18% identity in 275 aa overlap); Q9S7P1 epoxide hydrolase from Oryza sativa (Rice) (322 aa), FASTA scores: opt: 289, E(): 9.1e-11, (28.7% identity in 338 aa overlap); O23227|C7A10.830|AT4G36530 epoxide hydrolase from Arabidopsis thaliana (Mouse-ear cress) (378 aa) FASTA scores: opt: 287, E(): 1.4e-10, (26.1% identity in 272 aa overlap); Q21147|K02F3.6 epoxide hydrolase from Caenorhabditis elegans (386 aa), FASTA scores: opt: 283,E(): 2.5e-10, (33.35% identity in 156 aa overlap); etc. Also similar to P95276|EPHB|Rv1938|MTCY09F9.26c from Mycobacterium tuberculosis (356 aa), FASTA scores: opt: 296, E(): 3.6e-11, (29.7% identity in 340 aa overlap). Contains PS00213 Lipocalin signature. Similar to alpha/beta hydrolase fold.; Possible epoxide hydrolase EphE (epoxide hydratase) (arene-oxide hydratase) 4112329 ephE 885577 ephE Mycobacterium tuberculosis H37Rv Possible epoxide hydrolase EphE (epoxide hydratase) (arene-oxide hydratase) NP_218187.1 4111346 D 83332 CDS NP_218188.1 15610807 885176 complement(4112322..4113515) 1 NC_000962.3 Rv3671c, (MTV025.019c), len: 397 aa. Serine protease membrane protein, equivalent to Q9CB95|ML2298 putative membrane-associated serine protease from Mycobacterium leprae (401 aa), FASTA scores: opt: 2061, E(): 2.3e-108,(80.9% identity in 398 aa overlap). Also similar to many serine proteases, but generally with extended N-terminus,e.g. Q9X932|SCH5.30c putative serine protease (fragment) from Streptomyces coelicolor (385 aa), FASTA scores: opt: 835, E(): 1.2e-39, (39.9% identity in 386 aa overlap); Q9Z6T0|DEGP_CHLPN|HTRA|CPN0979|CP0877 probable serine protease do-like precursor from Chlamydia pneumoniae (Chlamydophila pneumoniae) (488 aa), FASTA scores: opt: 285, E(): 1e-08, (29.05% identity in 296 aa overlap); P73354|HTRA|SLR1204 serine protease from Synechocystis sp. strain PCC 6803 (452 aa), FASTA scores: opt: 284, E(): 1.1e-08, (29.55% identity in 308 aa overlap); Q9RWC4|DR0745 periplasmic serine protease, HTRA/DEGQ/DEGS family from Deinococcus radiodurans (366 aa), FASTA scores: opt: 271,E(): 4.9e-08, (35.45% identity in 206 aa overlap); etc. Also similar, but longer 114 aa at the N-terminus, to Q9S2P8|SC5F7.13 putative peptidase from Streptomyces coelicolor (282 aa), FASTA scores: opt: 594, E(): 3.1e-26,(38.95% identity in 285 aa overlap). And similar, but longer 146 aa at the N-terminus, to O07175|PEPA|Rv0125|MTCI418B.07 from Mycobacterium tuberculosis (355 aa), FASTA scores: opt: 295, E(): 2.2e-09, (29.55% identity in 254 aa overlap); and Q9CCY9|ML2659 probable secreted serine protease from Mycobacterium leprae FASTA scores: opt: 286, E(): 6.9e-09,(30.6% identity in 255 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Membrane-associated serine protease 4113515 885176 Rv3671c Mycobacterium tuberculosis H37Rv Membrane-associated serine protease NP_218188.1 4112322 R 83332 CDS NP_218189.1 15610808 885463 complement(4113521..4114342) 1 NC_000962.3 Rv3672c, (MTV025.020c), len: 273 aa. Conserved hypothetical protein, equivalent to Q9CB94|ML2299 hypothetical protein from Mycobacterium leprae (266 aa) FASTA scores: opt: 1358, E(): 5.2e-75, (76.4% identity in 267 aa overlap). Also similar to others (generally in C-terminal end) e.g. Q9XA45|SCH17.02c hypothetical 26.5 KDA protein from Streptomyces coelicolor (247 aa) FASTA scores: opt: 524, E(): 1.3e-24, (42.65% identity in 251 aa overlap); Q9AB27|CC0407 mutt/NUDIX family protein from Caulobacter crescentus (216 aa), FASTA scores: opt: 285,E(): 3.2e-10, (36.2% identity in 174 aa overlap); BAB49788|MLL2727|Q98HS8 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (204 aa), FASTA scores: opt: 278,E(): 8.1e-10, (31.45% identity in 151 aa overlap); P43337|YEAB_ECOLI|B1813 hypothetical 21.4 KDA protein from Escherichia coli strain K12 (192 aa) FASTA scores: opt: 252, E(): 2.9e-08, (35.9% identity in 170 aa overlap); etc. Contains PS01293 Uncharacterized protein family UPF0036 signature, LLT.; hypothetical protein 4114342 885463 Rv3672c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218189.1 4113521 R 83332 CDS NP_218190.1 15610809 885190 complement(4114474..4115157) 1 NC_000962.3 Rv3673c, (MTV025.021c), len: 227 aa. Possible membrane protein, thioredoxin-like protein (thiol-disulfide interchange protein), equivalent to Q9CB93|ML2300 putative membrane protein from Mycobacterium leprae (215 aa), FASTA scores: opt: 978, E(): 2.5e-52, (71.15% identity in 215 aa overlap). Some similarity with thioredoxin-related proteins e.g. P35160|RESA_BACSU RESA protein from Bacillus subtilis (181 aa), FASTA scores: opt: 212, E(): 5.7e-06, (30.55% identity in 108 aa overlap); Q9RXW6|DR0189 thiol:disulfide interchange protein from Deinococcus radiodurans (185 aa) FASTA scores: opt: 206, E(): 1.3e-05, (33.8% identity in 139 aa overlap); Q9I505|PA0953 probable thioredoxin from Pseudomonas aeruginosa (154 aa), FASTA scores: opt: 180,E(): 0.00044, (34.85% identity in 109 aa overlap); Q9KCP7|BH1522 thioredoxin (thiol:disulfide interchange protein) from Bacillus halodurans (177 aa), FASTA scores: opt: 178, E(): 0.00064, (31.75% identity in 107 aa overlap); P43221|TLPA_BRAJA thiol:disulfide interchange protein (cytochrome C biogenesis protein) from Bradyrhizobium japonicum (221 aa), FASTA scores: opt: 189,E(): 0.00017, (26.85% identity in 227 aa overlap); etc. Also similar to O06392|Rv0526|MTCY25D10.05 hypothetical 23.2 KDA protein from Mycobacterium tuberculosis (216 aa) FASTA scores: opt: 160, E(): 0.0093, (27.45% identity in 142 aa overlap). Contains PS00194 Thioredoxin family active site. Possibly belongs to the thioredoxin family.; Possible membrane-anchored thioredoxin-like protein (thiol-disulfide interchange related protein) 4115157 885190 Rv3673c Mycobacterium tuberculosis H37Rv Possible membrane-anchored thioredoxin-like protein (thiol-disulfide interchange related protein) NP_218190.1 4114474 R 83332 CDS NP_218191.2 57117142 885058 complement(4115157..4115894) 1 NC_000962.3 Rv3674c, (MT3775, MTV025.022c), len: 245 aa. Probable nth, endonuclease III (see citation below),equivalent to Q9CB92|nth|ML2301 putative endonuclease III from Mycobacterium leprae (272 aa), FASTA scores: opt: 1363, E(): 3.6e-81, (89.4% identity in 226 aa overlap). Also similar to many e.g. Q9XA44|SCH17.03c from Streptomyces coelicolor (250 aa), FASTA scores: opt: 937,E(): 2.2e-55, (61.65% identity in 219 aa overlap); P46303|UVEN_MICLU from Micrococcus luteus (Micrococcus lysodeikticus) (279 aa), FASTA scores: opt: 899, E(): 8.1e-53, (58.45% identity in 248 aa overlap); P73715|END3_SYNY3|nth|SLR1822 from Synechocystis sp. strain PCC 6803 (219 aa), FASTA scores: opt: 684, E(): 1.7e-38,(52.2% identity in 203 aa overlap); P39788|END3_BACSU|nth|JOOB from Bacillus subtilis (219 aa),FASTA scores: opt: 552, E(): 1.2e-29, (43.3% identity in 194 aa overlap); etc. Equivalent to AAK48142 from Mycobacterium tuberculosis strain CDC1551 (262 aa) but shorter 17 aa. Contains PS00764 Endonuclease III iron-sulfur binding region signature, and PS01155 Endonuclease III family signature. Belongs to the nth/MUTY family. Cofactor: binds a 4FE-4S cluster which is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand (by similarity). N-terminus extended since first submission (previously 226 aa).; Probable endonuclease III Nth (DNA-(apurinic or apyrimidinic site)lyase) (AP lyase) (AP endonuclease class I) (endodeoxyribonuclease (apurinic or apyrimidinic)) (deoxyribonuclease (apurinic or apyrimidinic)) 4115894 nth 885058 nth Mycobacterium tuberculosis H37Rv Probable endonuclease III Nth (DNA-(apurinic or apyrimidinic site)lyase) (AP lyase) (AP endonuclease class I) (endodeoxyribonuclease (apurinic or apyrimidinic)) (deoxyribonuclease (apurinic or apyrimidinic)) NP_218191.2 4115157 R 83332 CDS NP_218192.1 15610811 885155 4116002..4116379 1 NC_000962.3 Rv3675, (MTV025.023), len: 125 aa. Possible membrane protein, with some similarity to Q9YCZ2|APE1120 hypothetical 11.7 KDA protein from Aeropyrum pernix (103 aa), FASTA scores: opt: 100, E(): 9, (40.0% identity in 55 aa overlap).; Possible membrane protein 4116379 885155 Rv3675 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_218192.1 4116002 D 83332 CDS NP_218193.1 15610812 885502 4116478..4117152 1 NC_000962.3 Rv3676, (MTV025.024), len: 224 aa. Crp,transcriptional regulator belonging to crp/fnr family,identical to Q9CB91|ML2302 putative Crp/Fnr-family transcriptional regulator from Mycobacterium leprae (224 aa), FASTA scores: opt: 1408, E(): 8.8e-81, (95.95% identity in 224 aa overlap). Also highly similar to transcriptional regulators AAK58838 from Corynebacterium glutamicum (Brevibacterium flavum) (227 aa), FASTA scores: opt: 1178, E(): 1.9e-66, (79.9% identity in 224 aa overlap); and Q9XA42|SCH17.05 from Streptomyces coelicolor (224 aa), FASTA scores: opt: 869, E(): 3.4e-47, (54.45% identity in 224 aa overlap); and similar to others e.g. Q9RRX0|DR2362 from Deinococcus radiodurans (231 aa) FASTA scores: opt: 344, E(): 1.8e-14, (30.8% identity in 211 aa overlap); P29281|CRP_HAEIN from Haemophilus influenzae (224 aa), FASTA scores: opt: 330, E(): 1.3e-13, (32.25% identity in 189 aa overlap); P03020|CRP_ECOLI|cap|CSM|B3357 from Escherichia coli strain K12 and Shigella flexneri (210 aa),FASTA scores: opt: 323, E(): 3.5e-13, (32.25% identity in 189 aa overlap); etc. Contains helix-turn-helix motif at aa 175-196 (Score 1990, +5.96 SD). Belongs to the Crp/Fnr family of transcriptional regulators. Binds cAMP.; Transcriptional regulatory protein Crp (Crp/Fnr-family) 4117152 crp 885502 crp Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein Crp (Crp/Fnr-family) NP_218193.1 4116478 D 83332 CDS NP_218194.1 15610813 885079 complement(4117258..4118052) 1 NC_000962.3 Rv3677c, (MTV025.025c), len: 264 aa. Possible hydrolase, equivalent to Q9CB90|ML2303 putative hydrolase from Mycobacterium leprae (262 aa) FASTA scores: opt: 1400,E(): 8.5e-81, (82.05% identity in 262 aa overlap). Also similar to other hydrolases and hypothetical proteins e.g. Q9XA41|SCH17.06c putative hydrolase from Streptomyces coelicolor (256 aa) FASTA scores: opt: 609, E(): 3.9e-31,(54.65% identity in 247 aa overlap); Q9A9Q1|CC0923 metallo-beta-lactamase family protein from Caulobacter crescentus (297 aa), FASTA scores: opt: 306, E(): 4.7e-12,(35.45% identity in 268 aa overlap); Q9Y392 CGI-83 protein from Homo sapiens (Human) (288 aa), FASTA scores: opt: 281,E(): 1.7e-10, (33.2% identity in 259 aa overlap); Q9F7R6 predicted metallobeta lactamase fold protein from uncultured proteobacterium EBAC31A08 (265 aa), FASTA scores: opt: 257, E(): 5.1e-09, (32.55% identity in 252 aa overlap); Q9PBI4|XF2160 hydroxyacylglutathione hydrolase from Xylella fastidiosa (258 aa), FASTA scores: opt: 232,E(): 1.9e-07, (30.3% identity in 165 aa overlap); etc. Recombinant protein has beta lactamase activity (See Nampoothiri et al., 2008).; Possible hydrolase 4118052 885079 Rv3677c Mycobacterium tuberculosis H37Rv Possible hydrolase NP_218194.1 4117258 R 83332 CDS NP_218195.1 15610814 885495 complement(4118059..4118514) 1 NC_000962.3 Rv3678c, (MTV025.026c), len: 151 aa. Conserved protein, equivalent, but shorter 23 aa, to Q9CB89|ML2304 hypothetical protein from Mycobacterium leprae (174 aa),FASTA scores: opt: 746, E(): 2.1e-40, (78.15% identity in 151 aa overlap). Also highly similar to many hypothetical proteins or transcription regulators e.g. Q9XA38|SCH17.09c from Streptomyces coelicolor (155 aa), FASTA scores: opt: 637, E(): 1.5e-33, (69.1% identity in 152 aa overlap); BAB48205|MLR0658 from Rhizobium loti (Mesorhizobium loti) (154 aa), FASTA scores: opt: 500, E(): 6.8e-25, (55.35% identity in 150 aa overlap); BAB50615|MLR3802 transcription regulator from Rhizobium loti (Mesorhizobium loti) (153 aa), FASTA scores: opt: 425,E(): 3.8e-20, (44.35% identity in 151 aa overlap); Q9U0W7|L7276.02 from Leishmania major (163 aa) FASTA scores: opt: 404, E(): 8.5e-19, (47.7% identity in 151 aa overlap); Q9UZA3|PAB0825 putative translation initiation inhibitor from Pyrococcus abyssi (127 aa), FASTA scores: opt: 108, E(): 3.7, (30.75% identity in 130 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature.; hypothetical protein 4118514 885495 Rv3678c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218195.1 4118059 R 83332 CDS YP_178004.1 57117143 3205049 complement(4118530..4118691) 1 NC_000962.3 Rv3678A, len: 53 aa. Conserved hypothetical protein,similar to SCH17.10|AL079353_10 conserved hypothetical protein from Streptomyces coelicolor (53 aa), FASTA scores: opt: 259, E(): 1.5e-13, (78.0% identity in 50 aa overlap).; hypothetical protein 4118691 3205049 Rv3678A Mycobacterium tuberculosis H37Rv hypothetical protein YP_178004.1 4118530 R 83332 CDS NP_218196.1 15610815 885809 4118776..4119798 1 NC_000962.3 Rv3679, (MTV025.027), len: 340 aa. Probable anion transporting ATPase, equivalent to Q9CB88|ML2305 probable anion transporter protein from Mycobacterium leprae (341 aa), FASTA scores: opt: 1810, E(): 2.1e-98, (84.15% identity in 341 aa overlap). Also highly similar to Q9XA36|SCH17.11 putative ion-transporting ATPase from Streptomyces coelicolor (325 aa), FASTA scores: opt: 989,E(): 1.4e-50, (52.15% identity in 328 aa overlap); and similar to many anion transporting ATPases (principally arsenite transporters) e.g. O50593|ARSA_ACIMU arsenical pump-driving ATPase (arsenite-translocating ATPase) from Acidiphilium multivorum (583 aa), FASTA scores: opt: 225,E(): 8.1e-06, (25.1% identity in 319 aa overlap); AAG43231|ARSA arsenite activated ATPase from Salmonella typhimurium plasmid R46 FASTA scores: opt: 211, E(): 5.3e-05, (26.95% identity in 267 aa overlap); P52145|ARA2_ECOLI|ARSA arsenical pump-driving ATPase from Escherichia coli plasmid IncN R46 (583 aa), FASTA scores: opt: 211, E(): 5.3e-05, (26.95% identity in 267 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Some similarity to the ARSA ATPase family.; Probable anion transporter ATPase 4119798 885809 Rv3679 Mycobacterium tuberculosis H37Rv Probable anion transporter ATPase NP_218196.1 4118776 D 83332 CDS NP_218197.1 15610816 885317 4119795..4120955 1 NC_000962.3 Rv3680, (MTV025.028), len: 386 aa. Probable anion transporting ATPase, equivalent to Q9CB87|ML2306 probable anion transporter protein from Mycobacterium leprae (381 aa), FASTA scores: opt: 2131, E(): 6.5e-120, (88.1% identity in 370 aa overlap). Also highly similar, but shorter 29 aa, to Q9XA35|SCH17.12 putative ion-transporting ATPase from Streptomyces coelicolor (481 aa), FASTA scores: opt: 1190, E(): 1.1e-63, (51.25% identity in 441 aa overlap); and similar to many anion transporting ATPases e.g. Q9UZA6|PAB1555 anion transporting ATPase from Pyrococcus abyssi (330 aa) FASTA scores: opt: 242, E(): 3e-07, (24.6% identity in 297 aa overlap); Q9P7F8|SPAC1142.06 putative arsenite-translocating from Schizosaccharomyces pombe (Fission yeast) (329 aa), FASTA scores: opt: 239, E(): 4.5e-07, (27.9% identity in 197 aa overlap); Q9HS79|ARSA1|VNG0365G arsenical pump-driving ATPase from Halobacterium sp. strain NRC-1 (347 aa), FASTA scores: opt: 238, E(): 5.4e-07, (29.35% identity in 358 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; Probable anion transporter ATPase 4120955 885317 Rv3680 Mycobacterium tuberculosis H37Rv Probable anion transporter ATPase NP_218197.1 4119795 D 83332 CDS NP_218198.2 57117144 885320 complement(4121198..4121554) 1 NC_000962.3 Rv3681c, (MTV025.029c), len: 118 aa. Probable whiB4 (alternate gene name: whmA), WhiB-like regulatory protein (see Hutter & Dick 1999), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to ML2307 hypothetical protein from Mycobacterium leprae (116 aa). Also highly similar to Q9S2B9|SCH17.13c putative regulatory protein from Streptomyces coelicolor (112 aa), FASTA scores: opt: 392,E(): 1e-20, (67.95% identity in 78 aa overlap); Q9X951|WBLA hypothetical 14.3 KDA protein from Streptomyces coelicolor (129 aa), FASTA scores: opt: 392, E(): 1.1e-20, (67.95% identity in 78 aa overlap); Q9ACZ0|SCP1.161c putative regulatory protein from Streptomyces coelicolor (268 aa),FASTA scores: opt: 273, E(): 4.4e-12, (50.0% identity in 78 aa overlap); Q06387|WHIB-STV from Streptomyces griseocarneus (87 aa) FASTA scores: opt: 231, E(): 1.5e-09,(43.85% identity in 73 aa overlap); etc. Also similar to several putative regulator proteins from Mycobacterium tuberculosis e.g. MTCY7D11_7; MTCY78_13; MTCY10H4_23; MTCY1A6_6; and U00016_29 from Mycobacterium leprae. N-terminus shortened since first submission.; Probable transcriptional regulatory protein WhiB-like WhiB4 4121554 whiB4 885320 whiB4 Mycobacterium tuberculosis H37Rv Probable transcriptional regulatory protein WhiB-like WhiB4 NP_218198.2 4121198 R 83332 CDS YP_178005.1 57117145 885751 4121916..4124348 1 NC_000962.3 Rv3682, (MTV025.030), len: 810 aa. Probable ponA2,penicillin-binding protein (class A), bienzymatic membrane-associated protein with transglycosylase and transpeptidase activities. Almost identical to Q9CB85|PON1|ML2308 penicillin binding protein (class A) from Mycobacterium leprae (803 aa) FASTA scores: opt: 4743,E(): 3.3e-217, (87.7% identity in 806 aa overlap); or P72351|PON1|PBP1 high-molecular-mass class a penicillin binding protein from Mycobacterium leprae Cosmid B577 (821 aa), FASTA scores: opt: 4547, E(): 6.3e-208, (88.05% identity in 769 aa overlap) (see Basu et al., 1996). Also equivalent to a predicted homologous protein from Mycobacterium smegmatis. Also similar to others e.g. Q9XA34|SCH17.14 from Streptomyces coelicolor (428 aa; fragment), FASTA scores: opt: 727, E(): 2.3e-27, (36.55% identity in 413 aa overlap); Q9F9V7|PONA from Mycobacterium smegmatis (715 aa), FASTA scores: opt: 446, E(): 6.6e-14,(27.65% identity in 771 aa overlap) (see Billman-Jacobe et al., 1999); Q9CCY4|PONA|ML2688 from Mycobacterium leprae (708 aa), FASTA scores: opt: 413, E(): 2.4e-12, (26.8% identity in 660 aa overlap); Q9X6W0|PONB|MRCB|PA4700 from Pseudomonas aeruginosa (774 aa), FASTA scores: opt: 398,E(): 1.3e-11, (27.2% identity in 666 aa overlap); P45345|PBPB_HAEIN|MRCB|PONB|HI1725 (781 aa), FASTA scores: opt: 380, E(): 9.4e-11, (28.6% identity in 601 aa overlap); etc. Also similar to P71707|PONA1|Rv0050|MTCY21.13 probable bifunctional penicillin-binding protein 1A/1B (PBP1) from Mycobacterium tuberculosis (678 aa) FASTA scores: opt: 372,E(): 2e-10, (28.35% identity in 769 aa overlap). Seems to belong to the transglycosylase family in the N-terminal section, and to the transpeptidase family in the C-terminal section.; Probable bifunctional membrane-associated penicillin-binding protein 1A/1B PonA2 (murein polymerase) [includes: penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase)] 4124348 ponA2 885751 ponA2 Mycobacterium tuberculosis H37Rv Probable bifunctional membrane-associated penicillin-binding protein 1A/1B PonA2 (murein polymerase) [includes: penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase)] YP_178005.1 4121916 D 83332 CDS NP_218200.1 15610819 885780 4124417..4125376 1 NC_000962.3 Rv3683, (MTV025.031), len: 319 aa. Conserved protein, equivalent to Q9CB84|ML2309 hypothetical protein from Mycobacterium leprae (330 aa) FASTA scores: opt: 1791,E(): 9e-107, (85.45% identity in 296 aa overlap). Also similar to Q9X935|SCH66.03 conserved hypothetical protein from Streptomyces coelicolor (309 aa) FASTA scores: opt: 610, E(): 1.4e-31, (51.45% identity in 307 aa overlap); and Q9RRY7|YN45_DEIRA|DR2345 hypothetical protein from Deinococcus radiodurans (305 aa) FASTA scores: opt: 243,E(): 3.2e-08, (31.1% identity in 315 aa overlap) and some similarity to other hypothetical bacterial proteins e.g. Q9CF81|YQED from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (278 aa) FASTA scores: opt: 200,E(): 1.6e-05, (26.85% identity in 287 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 4125376 885780 Rv3683 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218200.1 4124417 D 83332 CDS NP_218201.3 448824817 885598 4125439..4126479 1 NC_000962.3 Rv3684, (MTV025.032), len: 346 aa. Probable lyase ,and more specifically a cysteine synthase, highly similar to many lyases e.g. Q9K3N2|SCG20A.08c putative lyase from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1469,E(): 3.7e-85, (63.35% identity in 341 aa overlap) (shorter 31 aa at N-terminus); Q9KT44|VC1061 cysteine synthase/ cystathionine beta-synthase family protein from Vibrio cholerae (355 aa), FASTA scores: opt: 1366, E(): 1.1e-78,(63.25% identity in 321 aa overlap); Q9I4R3|PA1061 hypothetical protein from Pseudomonas aeruginosa (365 aa),FASTA scores: opt: 1311, E(): 3.2e-75, (59.8% identity in 341 aa overlap); Q9PH18|XF0128 cysteine synthase from Xylella fastidiosa (390 aa), FASTA scores: opt: 1288, E(): 9.5e-74, (58.55% identity in 333 aa overlap) (shorter 34 aa at N-terminus); P55708|Y4XP_RHISN putative cysteine synthase from Rhizobium sp. strain NGR234 plasmid sym pNGR234a (336 aa), FASTA scores: opt: 376, E(): 2.1e-16,(29.2% identity in 315 aa overlap); etc. Equivalent to AAK48153 from Mycobacterium tuberculosis strain CDC1551 (368 aa) but shorter 22 aa.; Probable lyase 4126479 885598 Rv3684 Mycobacterium tuberculosis H37Rv Probable lyase NP_218201.3 4125439 D 83332 CDS NP_218202.1 15610821 885625 complement(4127295..4128725) 1 NC_000962.3 Rv3685c, (MTV025.033c), len: 476 aa. Probable cyp137, cytochrome P-450, similar to many e.g. Q9VXY0|C4S3_DROME|CYP4S3|CG9081 from Drosophila melanogaster (Fruit fly) (495 aa), FASTA scores: opt: 376,E(): 1.2e-15, (28.35% identity in 413 aa overlap); Q59163|CYP110A2 from Anabaena variabilis (459 aa) FASTA scores: opt: 320, E(): 3.1e-12, (31.4% identity in 411 aa overlap); O23051|C883_ARATH from Arabidopsis thaliana (Mouse-ear cress) (490 aa), FASTA scores: opt: 313, E(): 8.8e-12, (28.25% identity in 425 aa overlap); etc. Also similar to many from Mycobacterium tuberculosis e.g. O53765|C13B_MYCTU|CYP135B1|Rv0568|MT0594|MTV039.06 (472 aa), FASTA scores: opt: 920, E(): 4.6e-49, (36.25% identity in 447 aa overlap); P96813|C138_MYCTU|CYP138|Rv0136|MT0144|MTCI5.10 (441 aa) FASTA scores: opt: 886, E(): 5.3e-47, (35.5% identity in 445 aa overlap); etc. Belongs to the cytochrome P450 family.; Probable cytochrome P450 137 Cyp137 4128725 cyp137 885625 cyp137 Mycobacterium tuberculosis H37Rv Probable cytochrome P450 137 Cyp137 NP_218202.1 4127295 R 83332 CDS NP_218203.1 15610822 885306 complement(4128751..4129083) 1 NC_000962.3 Rv3686c, (MTV025.034c), len: 110 aa. Hypothetical protein, similar to P96893|Rv3288c|MTCY71.28c hypothetical 15.2 KDA protein from Mycobacterium tuberculosis (and Mycobacterium bovis) (137 aa) FASTA scores: opt: 106, E(): 5.6, (29.1% identity in 79 aa overlap); and a few hypothetical proteins e.g. Q9GUV6|L2259.2 from Leishmania major (360 aa) FASTA scores: opt: 118, E(): 2.1, (28.7% identity in 101 aa overlap). Equivalent to AAK48155 from Mycobacterium tuberculosis strain CDC1551 (166 aa) but shorter 56 aa.; hypothetical protein 4129083 885306 Rv3686c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218203.1 4128751 R 83332 CDS NP_218204.1 15610823 885599 complement(4129323..4129691) 1 NC_000962.3 Rv3687c, (MTV025.035c), len: 122 aa. RsfB,anti-anti-sigma factor (see citation below), showing some similarity to sporulation proteins and sigma-factor genes e.g. Q9WVX8|RSBV_STRCO|bldg|SCH5.12c anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa) FASTA scores: opt: 163, E(): 0.0007, (31.15% identity in 106 aa overlap); Q9F3A2|SC5F1.27c putative anti-sigma factor antagonist from Streptomyces coelicolor (114 aa) FASTA scores: opt: 159, E(): 0.0013, (29.8% identity in 104 aa overlap); P73609|SLR1859 hypothetical 12.0 KDA protein from Synechocystis sp. strain PCC 6803 (108 aa) FASTA scores: opt: 152, E(): 0.0034, (32.2% identity in 90 aa overlap); L47358|BACSPOI_1 spoIIA a from Paenibacillus polymyxa (117 aa), FASTA scores: opt: 107, E(): 0.23, (24.8% identity in 113 aa overlap); SQSIGB_4 rsbU, rsbV, rsbW & sigB genes from Steptomyces aureus (108 aa) (28.3% identity in 60 aa overlap); etc. Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. MTCY180_14 and MTCY441 _8.; Anti-anti-sigma factor RsfB (anti-sigma factor antagonist) (regulator of sigma F B) 4129691 rsfB 885599 rsfB Mycobacterium tuberculosis H37Rv Anti-anti-sigma factor RsfB (anti-sigma factor antagonist) (regulator of sigma F B) NP_218204.1 4129323 R 83332 CDS NP_218205.1 15610824 885563 complement(4129893..4130357) 1 NC_000962.3 Rv3688c, (MTV025.036c), len: 154 aa. Conserved protein, similar to other bacterial hypothetical proteins e.g. Q9X934|SCH66.02c from Streptomyces coelicolor (154 aa), FASTA scores: opt: 425, E(): 3.4e-19, (46.1% identity in 154 aa overlap); Q9WZF4|TM0690 from Thermotoga maritima (149 aa), FASTA scores: opt: 326, E(): 3.4e-13, (40.4% identity in 151 aa overlap); Q9PHU3|CJ0573 from Campylobacter jejuni (147 aa), FASTA scores: opt:290 , E(): 5.1e-11, (36.4% identity in 151 aa overlap); etc. Also some similarity to upstream O69654|Rv3686c|MTV025.034c conserved hypothetical protein from Mycobacterium tuberculosis.; hypothetical protein 4130357 885563 Rv3688c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218205.1 4129893 R 83332 CDS NP_218206.1 15610825 885107 4130357..4131712 1 NC_000962.3 Rv3689, (MTV025.037), len: 451 aa. Probable conserved transmembrane protein, with Proline rich N-terminus, similar to Q9KYW6|SCE33.17 putative integral membrane protein from Streptomyces coelicolor (462 aa) FASTA scores: opt: 730, E(): 2.7e-21, (38.1% identity in 412 aa overlap).; Probable conserved transmembrane protein 4131712 885107 Rv3689 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218206.1 4130357 D 83332 CDS NP_218207.1 15610826 885114 4131739..4132392 1 NC_000962.3 Rv3690, (MTV025.038), len: 217 aa. Probable conserved membrane protein, similar to Q9KYW5|SCE33.18 putative integral membrane protein from Streptomyces coelicolor (231 aa), FASTA scores: opt: 419, E(): 1.5e-19,(36.0% identity in 211 aa overlap). Equivalent to AAK48159 from Mycobacterium tuberculosis strain CDC1551 (233 aa) but shorter 16 aa.; Probable conserved membrane protein 4132392 885114 Rv3690 Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_218207.1 4131739 D 83332 CDS NP_218208.1 15610827 885623 4132518..4133519 1 NC_000962.3 Rv3691, (MTV025.039), len: 333 aa. Conserved protein, similar to Q9KYW4|SCE33.19 putative secreted protein from Streptomyces coelicolor (387 aa) FASTA scores: opt: 481, E(): 6e-23, (36.6% identity in 358 aa overlap). Equivalent to AAK48160 from Mycobacterium tuberculosis strain CDC1551 (381 aa) but shorter 48 aa.; hypothetical protein 4133519 885623 Rv3691 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218208.1 4132518 D 83332 CDS NP_218209.1 15610828 885323 4133516..4134592 1 NC_000962.3 Rv3692, (MTV025.040), len: 358 aa. Probable moxR2,methanol dehydrogenase regulatory protein, highly similar (generally longer at N-terminus) to Q9KYW3|SCE33.20 putative regulatory protein from Streptomyces coelicolor (329 aa), FASTA scores: opt: 1523, E(): 4.2e-74, (70.9% identity in 330 aa overlap); Q9Z538|SC9B2.21c putative regulatory protein from Streptomyces coelicolor (332 aa) FASTA scores: opt: 1008, E(): 1.1e-46, (50.8% identity in 313 aa overlap); Q9UZ67|MOXR-3|PAB0848 methanol dehydrogenase regulatory protein from Pyrococcus abyssi (314 aa), FASTA scores: opt: 989, E(): 1.1e-45, (50.65% identity in 302 aa overlap); Q9AAN1|CC0566 MOXR protein from Caulobacter crescentus (323 aa), FASTA scores: opt: 988, E(): 1.3e-45, (52.3% identity in 306 aa overlap); etc. Also similar to O53170|MTV007.26|MOXR|Rv1479 from Mycobacterium tuberculosis (377 aa); and O07392|AF002133_6|MOXR from Mycobacterium avium (309 aa). Also high similarity with several hypothetical bacterial proteins.; Probable methanol dehydrogenase transcriptional regulatory protein MoxR2 4134592 moxR2 885323 moxR2 Mycobacterium tuberculosis H37Rv Probable methanol dehydrogenase transcriptional regulatory protein MoxR2 NP_218209.1 4133516 D 83332 CDS NP_218210.1 15610829 885059 4134726..4136048 1 NC_000962.3 Rv3693, (MTV025.041), len: 440 aa (alternative start at 41910). Possible conserved membrane protein, similar to Q9KYW2|SCE33.21 putative lipoprotein from Streptomyces coelicolor (436 aa), FASTA scores: opt: 875, E(): 3.3e-46,(56.25% identity in 448 aa overlap); Q9AAN0|CC0567 hypothetical protein from Caulobacter crescentus (437 aa),FASTA scores: opt: 355, E(): 2.3e-14, (30.9% identity in 450 aa overlap); P73233|SLR2013 hypothetical 48.5 KDA protein from Synechocystis sp. strain PCC 6803 (435 aa),FASTA scores: opt: 340, E(): 1.9e-13, (29.7% identity in 438 aa overlap); etc. Equivalent to AAK48162 from Mycobacterium tuberculosis strain CDC1551 (475 aa) but shorter 35 aa. Also similar to other hypothetical proteins from Mycobacterium tuberculosis; MTV014_7; MTV007_27; and MTCY71_36 M. Predicted to be an outer membrane protein (See Song et al., 2008).; Possible conserved membrane protein 4136048 885059 Rv3693 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_218210.1 4134726 D 83332 CDS NP_218211.1 15610830 885578 complement(4136122..4137114) 1 NC_000962.3 Rv3694c, (MTV025.042c), len: 330 aa. Possible conserved transmembrane protein, highly similar to Q9KZM4|SCE34.01c putative integral membrane protein from Streptomyces coelicolor (335 aa), FASTA scores: opt: 1113,E(): 2.5e-60, (51.5% identity in 334 aa overlap); and similar to Q9KEW6|BH0733 hypothetical protein from Bacillus halodurans (355 aa), FASTA scores: opt: 381, E(): 6.1e-16,(24.15% identity in 331 aa overlap); Q9AAM9|CC0568 hypothetical protein from Caulobacter crescentus (332 aa),FASTA scores: opt: 352, E(): 3.3e-14, (30.3% identity in 310 aa overlap); P74166|SLR1478 hypothetical 35.4 KDA protein from Synechocystis sp. strain PCC 6803 (317 aa),FASTA scores: opt: 330, E(): 6.8e-13, (25.65% identity in 308 aa overlap); etc. C-terminal end shows similarity to O29631|AF0624|AE001061_10 conserved hypothetical protein (putative nifU protein) from Archaeoglobus fulgidus (185 aa), FASTA scores: opt: 154, E(): 0.021, (29.0% identity in 131 aa overlap). Equivalent to AAK48163 from Mycobacterium tuberculosis strain CDC1551 (395 aa) but shorter 65 aa. Also some similarity to MTCY428_20 hypothetical 43.7 KDA protein from Mycobacterium tuberculosis.; Possible conserved transmembrane protein 4137114 885578 Rv3694c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_218211.1 4136122 R 83332 CDS NP_218212.1 15610831 885538 4137206..4138138 1 NC_000962.3 Rv3695, (MTV025.043), len: 310 aa. Possible conserved membrane protein, equivalent, but longer 88 aa,to Q9CB83|ML2312 possible membrane protein from Mycobacterium leprae (196 aa), FASTA scores: opt: 898, E(): 5.2e-36, (71.05% identity in 190 aa overlap). Also highly similar to Q9KZM3|SCE34.02 putative integral membrane protein from Streptomyces coelicolor (318 aa), FASTA scores: opt: 740,E(): 2.4e-28, (43.25% identity in 319 aa overlap); and similar to P72718|SLR0254 hypothetical 30.4 KDA protein from Synechocystis sp. strain PCC 6803 (266 aa), FASTA scores: opt: 287, E(): 6.1e-07, (29.6% identity in 260 aa overlap); Q9HW83|PA4318 hypothetical protein from Pseudomonas aeruginosa (265 aa), FASTA scores: opt: 250,E(): 3.5e-05, (32.0% identity in 203 aa overlap); Q9KEW5|BH0734 hypothetical protein from Bacillus halodurans (266 aa), FASTA scores: opt: 168, E(): 0.0047, (25.95% identity in 231 aa overlap); etc. C-terminal end shows some similarity to proline-rich proteins e.g. Q62106 proline-rich salivary protein (fragment) from Mus musculus (Mouse) (188 aa) (36.1% identity in 97 aa overlap). Equivalent to AAK48164 from Mycobacterium tuberculosis strain CDC1551 (269 aa) but longer 41 aa.; Possible conserved membrane protein 4138138 885538 Rv3695 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_218212.1 4137206 D 83332 CDS NP_218213.1 15610832 885280 complement(4138202..4139755) 1 NC_000962.3 Rv3696c, (MTV025.044c), len: 517 aa. Probable glpK,glycerol kinase, equivalent to Q9CB81|GLPK_MYCLE|ML2314 glycerol kinase from Mycobacterium leprae (508 aa), FASTA scores: opt: 3120, E(): 4.7e-189, (91.35% identity in 508 aa overlap). Also highly similar to others e.g. Q9RJM2|GLPK from Streptomyces coelicolor (507 aa), FASTA scores: opt: 2606, E(): 1.1e-156, (75.35% identity in 503 aa overlap); Q9ADA7|GLPK from Streptomyces coelicolor (512 aa) FASTA scores: opt: 2002, E(): 1.3e-118, (59.05% identity in 503 aa overlap); Q9X1E4|GLK2_THEMA|TM1430 from Thermotoga maritima (496 aa), FASTA scores: opt: 1838, E(): 2.7e-108,(54.8% identity in 498 aa overlap); P08859|GLPK_ECOLI|B3926 from Escherichia coli strain K12 (501 aa), FASTA scores: opt: 1740, E(): 4.1e-102, (52.3% identity in 499 aa overlap); etc. Contains PS00933 FGGY family of carbohydrate kinases signature 1, PS00070 Aldehyde dehydrogenases cysteine active site, PS00445 FGGY family of carbohydrate kinases signature 2. Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family.; Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) (glycerokinase) (GK) 4139755 glpK 885280 glpK Mycobacterium tuberculosis H37Rv Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) (glycerokinase) (GK) NP_218213.1 4138202 R 83332 CDS NP_218214.1 15610833 885492 complement(4139805..4140242) 1 NC_000962.3 Rv3697c, (MTV025.045c), len: 145 aa. Possible vapC48, toxin, part of toxin-antitoxin (TA) operon with Rv3697A, contains PIN domain, see Arcus et al. 2005. Similar to many others in Mycobacterium tuberculosis e.g. Q10800|YS72_MYCTU|Rv2872|MT2939|MTCY274.03 (147 aa) FASTA scores: opt: 223, E(): 7.3e-08, (32.6% identity in 141 aa overlap); O53501|Rv2103c|MTV020.03 (144 aa), FASTA scores: opt: 215, E(): 2.4e-07, (31.4% identity in 137 aa overlap); O53812|Rv0749|MTV041.23 (142 aa), FASTA scores: opt: 192,E(): 7.6e-06, (31.25% identity in 144 aa overlap); etc.; Possible toxin VapC48 Contains PIN domain 4140242 vapC48 885492 vapC48 Mycobacterium tuberculosis H37Rv Possible toxin VapC48 Contains PIN domain NP_218214.1 4139805 R 83332 CDS YP_007412423.1 448818550 14515904 complement(4140239..4140463) 1 NC_000962.3 Rv3697A, len: 74 aa. Possible vapB48, antitoxin,part of toxin-antitoxin (TA) operon with Rv3697c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3321c, Rv0748; Possible antitoxin VapB48 4140463 vapB48 14515904 vapB48 Mycobacterium tuberculosis H37Rv Possible antitoxin VapB48 YP_007412423.1 4140239 R 83332 CDS NP_218215.1 15610834 885565 4140493..4142022 1 NC_000962.3 Rv3698, (MTV025.046), len: 509 aa. Conserved protein, highly similar to Q9AK89|SC10A9.15c conserved hypothetical protein from Streptomyces coelicolor (505 aa),FASTA scores: opt: 1720, E(): 9e-103, (53.65% identity in 494 aa overlap). N-terminal end highly similar to CAC42136|SCBAC25F8.01 conserved hypothetical protein (fragment) from Streptomyces coelicolor (291 aa), FASTA scores: opt: 1078, E(): 8.7e-62, (52.6% identity in 291 aa overlap); and C-terminus highly similar to CAC44687|SCBAC17A6.42c (235 aa), FASTA scores: opt: 911,E(): 3.8e-51, (57.25% identity in 234 aa overlap).; hypothetical protein 4142022 885565 Rv3698 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218215.1 4140493 D 83332 CDS NP_218216.1 15610835 885779 4142044..4142745 1 NC_000962.3 Rv3699, (MTV025.047), len: 233 aa. Conserved protein, showing similarity with hypothetical proteins e.g. Q9P3V6|SPAC1348.04 (alias Q9P3E7|SPAC750.03c or Q9P7U5|SPAC977.03) from Schizosaccharomyces pombe (Fission yeast) (145 aa), FASTA scores: opt: 188, E(): 7.5e-05,(31.65% identity in 120 aa overlap); and Q9KB70|BH2058 from Bacillus halodurans (241 aa) FASTA scores: opt: 185, E(): 0.00018, (27.8% identity in 162 aa overlap); Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 166,E(): 0.0025, (29.95% identity in 147 aa overlap); etc. Also highly similar to O06426|Rv0560c|MTCY25D10.39c hypothetical 25.9 KDA protein from Mycobacterium tuberculosis (241 aa),FASTA scores: opt: 690, E(): 6.5e-36, (53.4% identity in 234 aa overlap); and similar to other hypothetical proteins from Mycobacterium tuberculosis e.g. P71805|Rv1377c|MTCY02B12.11c (212 aa) FASTA scores: opt: 378, E(): 1.5e-16, (35.4% identity in 192 aa overlap); P71972|Rv2675c|MTCY441.44c (250 aa) FASTA scores: opt: 297,E(): 2e-11, (31.1% identity in 193 aa overlap); etc.; hypothetical protein 4142745 885779 Rv3699 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218216.1 4142044 D 83332 CDS NP_218217.1 15610836 885161 complement(4142748..4143920) 1 NC_000962.3 Rv3700c, (MTV025.048c), len: 390 aa. Conserved hypothetical protein; could be a transferase or a lyase. Indeed, similar to various enzymes e.g. Q53824|CAC capreomycin acetyltransferase from Streptomyces capreolus (359 aa), FASTA scores: opt: 338, E(): 1.1e-12, (33.35% identity in 363 aa overlap); Q9HXX3|CSD_PSEAE|PA3667 probable cysteine desulfurase from Pseudomonas aeruginosa (401 aa) FASTA scores: opt: 260, E(): 4.8e-08, (30.2% identity in 404 aa overlap); Q9X815|SC6G10.30 putative aminotransferase from Streptomyces coelicolor (460 aa),FASTA scores: opt: 243, E(): 5.4e-07, (29.15% identity in 374 aa overlap); Q9A761|CC1865 aminotransferase class V from Caulobacter crescentus (379 aa), FASTA scores: opt: 234, E(): 1.6e-06, (27.95% identity in 383 aa overlap); O74351|NFS1_SCHPO|SPBC21D10.11c probable cysteine desulfurase from Schizosaccharomyces pombe (Fission yeast) (498 aa), FASTA scores: opt: 232, E(): 2.5e-06, (29.1% identity in 285 aa overlap); Q9RME8|NIFS NIFS protein (cysteine desulfurase, tRNA splicing protein) from Zymomonas mobilis (370 aa), FASTA scores: opt: 230, E(): 2.6e-06, (32.85% identity in 201 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; hypothetical protein 4143920 885161 Rv3700c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218217.1 4142748 R 83332 CDS NP_218218.1 15610837 885521 complement(4143951..4144916) 1 NC_000962.3 Rv3701c, (MTV025.049c), len: 321 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9RCZ8|SCM1.46 from Streptomyces coelicolor (251 aa), FASTA scores: opt: 897, E(): 1.1e-50, (59.9% identity in 242 aa overlap); P73759|SLR0865 from Synechocystis sp. strain PCC 6803 (337 aa), FASTA scores: opt: 779, E(): 5.7e-43, (40.35% identity in 327 aa overlap); Q9GWA1|LM12.997 from Leishmania major (383 aa) FASTA scores: opt: 616, E(): 2.1e-32, (39.05% identity in 297 aa overlap); etc.; hypothetical protein 4144916 885521 Rv3701c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218218.1 4143951 R 83332 CDS NP_218219.1 15610838 885224 complement(4144913..4145614) 1 NC_000962.3 Rv3702c, (MTV025.050c), len: 233 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins Q9RCZ9|SCM1.45 from Streptomyces coelicolor (271 aa), FASTA scores: opt: 383, E(): 2.3e-17, (44.85% identity in 252 aa overlap); and P54004|Y199_SYNY3|SLR0199 from Synechocystis sp. strain PCC 6803 (304 aa), FASTA scores: opt: 292, E(): 1.7e-11, (30.05% identity in 263 aa overlap); and similar to others e.g. Q9KMU4|VCA0225 from Vibrio cholerae (254 aa), FASTA scores: opt: 260, E(): 1.6e-09, (29.8% identity in 245 aa overlap). Equivalent to AAK48172 from Mycobacterium tuberculosis strain CDC1551 (194 aa) but longer 39 aa.; hypothetical protein 4145614 885224 Rv3702c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218219.1 4144913 R 83332 CDS NP_218220.1 15610839 885128 complement(4145614..4146891) 1 NC_000962.3 Rv3703c, (MTV025.051c), len: 425 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. Q9RD00|SCM1.44 from Streptomyces coelicolor (446 aa), FASTA scores: opt: 1480, E(): 1.4e-85, (53.9% identity in 421 aa overlap); P72841|SLR1303 from Synechocystis sp. strain PCC 6803 (410 aa), FASTA scores: opt: 533, E(): 4.5e-26, (36.6% identity in 429 aa overlap); Q9KYH7|SCC61A.16 from Streptomyces coelicolor (256 aa),FASTA scores: opt: 266, E(): 1.9e-09, (32.25% identity in 248 aa overlap); etc. Also similar to P95060|Rv0712|MTCY210.31 hypothetical 32.7 KDA protein from Mycobacterium tuberculosis (299 aa), FASTA scores: opt: 243, E(): 5.9e-08, (30.6% identity in 304 aa overlap).; hypothetical protein 4146891 885128 Rv3703c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218220.1 4145614 R 83332 CDS NP_218221.1 15610840 885053 complement(4146888..4148186) 1 NC_000962.3 Rv3704c, (MTV025.052c), len: 432 aa. Possible gshA,glutamate--cysteine ligase, similar to many e.g. Q9A2Z2|CC3414 glutamate--cysteine ligase from Caulobacter crescentus (453 aa), FASTA scores: opt: 404, E(): 5.9e-17,(30.45% identity in 312 aa overlap); Q9SEH0|GSH1 gamma-glutamylcysteinyl synthetase precursor from Pisum sativum (Garden pea) (499 aa), FASTA scores: opt: 400, E(): 1.1e-16, (26.4% identity in 439 aa overlap); Q9RH09|GSH gamma-glutamylcysteine synthetase from Zymomonas mobilis (462 aa), FASTA scores: opt: 397, E(): 1.6e-16, (28.95% identity in 304 aa overlap); P46309|GSH1_ARATH|GSH1|AT4G23100|F7H19.290 glutamate--cysteine ligase from Arabidopsis thaliana (Mouse-ear cress) (522 aa), FASTA scores: opt: 395, E(): 2.3e-16, (27.25% identity in 385 aa overlap); etc. But note that this putative protein is also similar to Q9JMV4|GSHA putative glutathione synthetase (fragment) from Bradyrhizobium japonicum (460 aa), FASTA scores: opt: 498,E(): 1.3e-22, (33.35% identity in 333 aa overlap) (no significant publications found (August 2001)). Nucleotide position 4147070 in the genome sequence has been corrected,A:G resulting in L373L.; Glutamate--cysteine ligase GshA (gamma-glutamylcysteine synthetase) (gamma-ECS) (GCS) (gamma-glutamyl-L-cysteine synthetase) 4148186 gshA 885053 gshA Mycobacterium tuberculosis H37Rv Glutamate--cysteine ligase GshA (gamma-glutamylcysteine synthetase) (gamma-ECS) (GCS) (gamma-glutamyl-L-cysteine synthetase) NP_218221.1 4146888 R 83332 CDS NP_218222.1 15610841 885229 complement(4148318..4148962) 1 NC_000962.3 Rv3705c, (MTV025.053c), len: 214 aa. Conserved protein, equivalent to Q9CB80|ML2320 hypothetical protein from Mycobacterium leprae (215 aa) FASTA scores: opt: 1145,E(): 5.9e-68, (79.45% identity in 214 aa overlap). Some similarity to the C-terminal end of Q11053|PKNH_MYCTU|Rv1266c|MT1304|MTCY50.16 probable serine/threonine-protein from Mycobacterium tuberculosis (626 aa), FASTA scores: opt: 175, E(): 0.0005, (24.9% identity in 201 aa overlap); and to the N-terminal end of P23903|E13B_BACCI|GLCA glucan endo-1,3-beta-glucosidase A1 precursor from Bacillus circulans (682 aa), FASTA scores: opt: 122, E(): 1.6, (25.6% identity in 164 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 4148962 885229 Rv3705c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218222.1 4148318 R 83332 CDS YP_178006.1 57117146 3205111 complement(4149091..4149480) 1 NC_000962.3 Rv3705A, len: 129 aa. Conserved hypothetical protein, similar to downstream ORF O69674|Rv3706c|MTV025.054c conserved hypothetical proline rich protein from Mycobacterium tuberculosis (106 aa),FASTA scores: opt: 245, E(): 0.00013, (40.7% identity in 113 aa overlap).; Conserved hypothetical proline rich protein 4149480 3205111 Rv3705A Mycobacterium tuberculosis H37Rv Conserved hypothetical proline rich protein YP_178006.1 4149091 R 83332 CDS NP_218223.1 15610842 885222 complement(4149591..4149911) 1 NC_000962.3 Rv3706c, (MTV025.054c), len: 106 aa. Conserved ypothetical pro-rich protein, similar to upstream ORF Rv3705A (129 aa), and AAK48176|MT3808.1 hypothetical 13.0 KDA protein from Mycobacterium tuberculosis strain CDC1551 (129 aa), FASTA scores: opt: 245, E(): 4.4e-06, (40.7% identity in 113 aa overlap).; Conserved hypothetical proline rich protein 4149911 885222 Rv3706c Mycobacterium tuberculosis H37Rv Conserved hypothetical proline rich protein NP_218223.1 4149591 R 83332 CDS NP_218224.1 15610843 885587 complement(4150030..4151040) 1 NC_000962.3 Rv3707c, (MTV025.055c), len: 336 aa. Equivalent to Q9CB79|ML2321 hypothetical protein from Mycobacterium leprae (336 aa), FASTA scores: opt: 1948, E(): 6.7e-110,(81.95% identity in 332 aa overlap); and P41402|YASD_MYCSM hypothetical 35.9 KDA protein in the aspartokinase gene cluster from Mycobacterium smegmatis (333 aa), FASTA scores: opt: 1731, E(): 7.4e-97, (70.85% identity in 333 aa overlap).; hypothetical protein 4151040 885587 Rv3707c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218224.1 4150030 R 83332 CDS NP_218225.1 15610844 885118 complement(4151180..4152217) 1 NC_000962.3 Rv3708c, (MTV025.056c), len: 345 aa. Asd,aspartate-semialdehyde dehydrogenase (see citation below),equivalent to many e.g. P47730|DHAS_MYCBO|ASD from Mycobacterium bovis (345 aa) FASTA scores: opt: 2150, E(): 1.6e-124, (97.7% identity in 345 aa overlap); or Q9JN40|ASD from Mycobacterium bovis (323 aa), FASTA scores: opt: 2021,E(): 1.2e-116, (97.5% identity in 323 aa overlap); Q9CB78|ASD|ML2322 from Mycobacterium leprae (351 aa), FASTA scores: opt: 1889, E(): 1.6e-108, (84.45% identity in 347 aa overlap); P41404|DHAS_MYCSM|ASD from Mycobacterium smegmatis (346 aa), FASTA scores: opt: 1801, E(): 3.9e-103,(80.3% identity in 345 aa overlap); etc. Contains PS01103 Aspartate-semialdehyde dehydrogenase signature. Belongs to the aspartate-semialdehyde dehydrogenase family.; Aspartate-semialdehyde dehydrogenase Asd (ASA dehydrogenase) (ASADH) (aspartic semialdehyde dehydrogenase) (L-aspartate-beta-semialdehyde dehydrogenase) 4152217 asd 885118 asd Mycobacterium tuberculosis H37Rv Aspartate-semialdehyde dehydrogenase Asd (ASA dehydrogenase) (ASADH) (aspartic semialdehyde dehydrogenase) (L-aspartate-beta-semialdehyde dehydrogenase) NP_218225.1 4151180 R 83332 CDS NP_218226.1 15610845 885223 complement(4152218..4153483) 1 NC_000962.3 Rv3709c, (MTV025.057c), len: 421 aa. Ask,aspartokinase (see citation below), equivalent to Q9CB77|ask|ML2323 from Mycobacterium leprae (421 aa), FASTA scores: opt: 2531, E(): 2e-140, (92.65% identity in 421 aa overlap); and P41403|AK_MYCSM|ask from Mycobacterium smegmatis (421 aa), FASTA scores: opt: 2423, E(): 4e-134,(88.1% identity in 421 aa overlap); and to several other organisms e.g. Q9RQ25|ASKA from Amycolatopsis mediterranei (421 aa), FASTA scores: opt: 2026, E(): 5.8e-111, (72.2% identity in 421 aa overlap). Contains PS00324 Aspartokinase signature. Belongs to the aspartokinase family. Alternative products: the alpha and beta subunits of aspartokinase are produced by the use of alternative initiation sites (by similarity).; Aspartokinase Ask (aspartate kinase) [contains: aspartokinase alpha subunit (Ask-alpha); and aspartokinase beta subunit (Ask-beta)] 4153483 ask 885223 ask Mycobacterium tuberculosis H37Rv Aspartokinase Ask (aspartate kinase) [contains: aspartokinase alpha subunit (Ask-alpha); and aspartokinase beta subunit (Ask-beta)] NP_218226.1 4152218 R 83332 CDS NP_218227.3 345462027 885092 4153740..4155674 1 NC_000962.3 Rv3710, (MTV025.058), len: 644 aa. LeuA,alpha-isopropylmalate synthase (see citations below),equivalent to Q9CB76|LEUA|ML2324 2-isopropylmalate synthase from Mycobacterium leprae (607 aa), FASTA scores: opt: 3291, E(): 3.7e-192, (80.7% identity in 642 aa overlap). Also highly similar to many e.g. P42455|LEU1_CORGL|LEUA from Corynebacterium glutamicum (Brevibacterium flavum) (616 aa), FASTA scores: opt: 2547, E(): 5.3e-147, (63.25% identity in 645 aa overlap); O31046|LEU1_STRCO|LEUA from Streptomyces coelicolor (573 aa), FASTA scores: opt: 2226,E(): 1.5e-127, (57.8% identity in 616 aa overlap); BAB49833|Q98HN3|MLR2792 from Rhizobium loti (Mesorhizobium loti) (588 aa), FASTA scores: opt: 1849, E(): 1.1e-104,(58.0% identity in 536 aa overlap); etc. Equivalent to AAK48181 from Mycobacterium tuberculosis strain CDC1551 (659 aa) but shorter 15 aa. Contains PS00815 and PS00816 Alpha-isopropylmalate and homocitrate synthases signatures 1 and 2. Belongs to the alpha-IPM synthetase / homocitrate synthase family. K+ is likely the physiological activator; Zn2+ and Cd2+ are inhibitors.; 2-isopropylmalate synthase LeuA (alpha-isopropylmalate synthase) (alpha-IPM synthetase) (IPMS) 4155674 leuA 885092 leuA Mycobacterium tuberculosis H37Rv 2-isopropylmalate synthase LeuA (alpha-isopropylmalate synthase) (alpha-IPM synthetase) (IPMS) NP_218227.3 4153740 D 83332 CDS NP_218228.1 15610847 885088 complement(4155740..4156729) 1 NC_000962.3 Rv3711c, (MTV025.059c), len: 329 aa. Probable dnaQ,DNA polymerase III, epsilon subunit, similar to many e.g. Q9RJ41|SCI8.12 from Streptomyces coelicolor (328 aa), FASTA scores: opt: 509, E(): 4.2e-25, (41.6% identity in 315 aa overlap); Q9JYS6|NMB1451 from Neisseria meningitidis (serogroup B) (and Q9JTR5|MA1665 from serogroup A) (470 aa), FASTA scores: opt: 247, E(): 2.6e-08, (33.15% identity in 172 aa overlap); O83649|DP3E_TREPA|DNAQ|TP0643 from Treponema pallidum (215 aa), FASTA scores: opt: 240, E(): 3.7e-08, (29.65% identity in 162 aa overlap); P03007|DP3E_ECOLI|MUTD|B0215 from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 208, E(): 4.5e-06, (28.4% identity in 169 aa overlap); etc. Also similar to Q10384|YL91_MYCTU|Rv2191|MTCY190.02 from Mycobacterium tuberculosis (645 aa), FASTA scores: opt: 260, E(): 5e-09,(28.55% identity in 301 aa overlap).; Probable DNA polymerase III (epsilon subunit) DnaQ 4156729 dnaQ 885088 dnaQ Mycobacterium tuberculosis H37Rv Probable DNA polymerase III (epsilon subunit) DnaQ NP_218228.1 4155740 R 83332 CDS NP_218229.1 15610848 885228 4156981..4158222 1 NC_000962.3 Rv3712, (MTV025.060), len: 413 aa. Possible ligase ,equivalent to O69522|ML2326|MLCB2407.24c hypothetical 43.8 KDA protein (possible ligase) from Mycobacterium leprae (411 aa), FASTA scores: opt: 2265, E(): 8e-129, (84.25% identity in 413 aa overlap). Also similar to ligases or hypothetical proteins e.g. Q9FCA1|2SCG58.12 putative ligase from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1168, E(): 6.7e-63, (45.8% identity in 406 aa overlap); P74303|SLR0938 hypothetical 50.2 KDA protein from Synechocystis sp. strain PCC 6803 (459 aa), FASTA scores: opt: 392, E(): 3.1e-16, (28.45% identity in 397 aa overlap); Q99ZX1|SPY1035 putative UDP-N-acetylmuramyl tripeptide synthetase from Streptococcus pyogenes (445 aa),FASTA scores: opt: 335, E(): 8.1e-13, (29.2% identity in 438 aa overlap); Q9CGJ0|YLBD hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (449 aa), FASTA scores: opt: 324, E(): 3.8e-12, (28.75% identity in 445 aa overlap); Q9ZGG7|MURC UDP-N-acetylmuramyl tripeptide synthetase from Heliobacillus mobilis (455 aa), FASTA scores: opt: 292,E(): 3.2e-10, (30.75% identity in 449 aa overlap); etc.; Possible ligase 4158222 885228 Rv3712 Mycobacterium tuberculosis H37Rv Possible ligase NP_218229.1 4156981 D 83332 CDS NP_218230.1 15610849 885584 4158227..4158922 1 NC_000962.3 Rv3713, (MTV025.061), len: 231 aa. Possible cobQ2,cobyric acid synthase, equivalent to O69521|ML2327|MLCB2407.23c hypothetical 24.5 KDA protein from Mycobacterium leprae (230 aa), FASTA scores: opt: 1313, E(): 4.7e-73, (86.1% identity in 230 aa overlap). Also partially similar to several cobyric acid synthases and hypothetical proteins e.g. Q9FCA0|2SCG58.13 hypothetical 26.2 KDA protein from Streptomyces coelicolor (242 aa), FASTA scores: opt: 639, E(): 6.2e-32, (46.6% identity in 234 aa overlap); Q9ZGG8|COBQ cobyric acid synthase from Heliobacillus mobilis (252 aa), FASTA scores: opt: 501, E(): 1.7e-23, (40.75% identity in 206 aa overlap); BAB58053|SAV1891 hypothetical 27.4 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (243 aa),FASTA scores: opt: 400, E(): 2.3e-17, (35.95% identity in 217 aa overlap); Q9CGJ1|COBQ cobyric acid synthase from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (261 aa), FASTA scores: opt: 353, E(): 1.8e-14, (35.3% identity in 201 aa overlap); O26880|COBQ_METTH|MTH787 probable cobyric acid synthase from Methanobacterium thermoautotrophicum (504 aa), FASTA scores: opt: 201, E(): 5.6e-05, (33.35% identity in 171 aa overlap); etc. Also similar to hypothetical mycobacterial proteins O05811|COBB_MYCTU|Rv2848c|MT2914|MTCY24A1.09 (457 aa) and P71842|Rv0789c|MTCY369.33c (199 aa). Seems to belong to the COBB/COBQ family, COBQ subfamily.; Possible cobyric acid synthase CobQ2 4158922 cobQ2 885584 cobQ2 Mycobacterium tuberculosis H37Rv Possible cobyric acid synthase CobQ2 NP_218230.1 4158227 D 83332 CDS NP_218231.1 15610850 885456 complement(4158931..4159821) 1 NC_000962.3 Rv3714c, (MTV025.062c), len: 296 aa. Conserved hypothetical protein, highly similar to O07396|MAV346 MAV346 protein from Mycobacterium avium (346 aa) FASTA scores: opt: 834, E(): 2.2e-46, (50.0% identity in 286 aa overlap); and also highly similar to several proteins from Mycobacterium tuberculosis e.g. O53421|Rv1073|MTV017.26 (283 aa), FASTA scores: opt: 869, E(): 1e-48, (51.1% identity in 270 aa overlap); P71763|Rv1482c|MTCY277.03c (339 aa), FASTA scores: opt: 775, E(): 1.3e-42, (46.35% identity in 289 aa overlap); P96837|Rv3555c|MTCY06G11.02c (289 aa), FASTA scores: opt: 733, E(): 5.9e-40, (44.15% identity in 281 aa overlap); etc. Partially similar to Q9Z512|UVRC_STRCO|SCC54.13c excinuclease ABC subunit C from Streptomyces coelicolor (728 aa), FASTA scores: opt: 122,E(): 2.5, (27.0% identity in 174 aa overlap). Equivalent to AAK48186 from Mycobacterium tuberculosis strain CDC1551 (341 aa) but shorter 45 aa.; hypothetical protein 4159821 885456 Rv3714c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218231.1 4158931 R 83332 CDS NP_218232.1 15610851 885307 complement(4159889..4160500) 1 NC_000962.3 Rv3715c, (MTV025.063c), len: 203 aa. Probable recR,recombination protein (see citation below), equivalent to O69520|RECR_MYCLE|ML2329|MLCB2407.21 recombination protein from Mycobacterium leprae (203 aa), FASTA scores: opt: 1246, E(): 9.2e-71, (91.6% identity in 202 aa overlap). Also highly similar to many e.g. Q9XAI4|RECR_STRCO|SC66T3.29c from Streptomyces coelicolor (199 aa), FASTA scores: opt: 952, E(): 1.9e-52, (68.3% identity in 202 aa overlap); P24277|RECR_BACSU|RECM|recd from Bacillus subtilis (198 aa), FASTA scores: opt: 696,E(): 1.8e-36, (50.5% identity in 198 aa overlap); Q9ZNA2|RECR_DEIRA|DR0198 from Deinococcus radiodurans (220 aa), FASTA scores: opt: 673, E(): 5.2e-35, (49.75% identity in 195 aa overlap); etc. Belongs to the RECR family.; Probable recombination protein RecR 4160500 recR 885307 recR Mycobacterium tuberculosis H37Rv Probable recombination protein RecR NP_218232.1 4159889 R 83332 CDS NP_218233.1 15610852 885595 complement(4160512..4160913) 1 NC_000962.3 Rv3716c, (MTV025.064c), len: 133 aa. Conserved protein, equivalent to O69519|Y1B6_MYCLE|ML2330|MLCB2407.20 hypothetical 11.9 KDA protein from Mycobacterium leprae (116 aa), FASTA scores: opt: 616, E(): 2.6e-21, (84.55% identity in 110 aa overlap). Also highly similar to hypothetical ~12 kDa proteins in the vicinity of recR from other bacteria e.g. Q9XAI3|YT3D_STRCO|SC66T3.30c hypothetical 11.7 KDA protein from Streptomyces coelicolor (115 aa), FASTA scores: opt: 379, E(): 9.5e-11, (50.8% identity in 122 aa overlap); BAB56641|SAV0479 conserved hypothetical protein from Staphylococcus aureus subsp. aureus Mu50 (105 aa) FASTA scores: opt: 295, E(): 4.9e-07,(41.75% identity in 103 aa overlap); Q99WC4P24281|YAAK_BACSU hypothetical 11.8 KDA protein in DNAZ-RECR intergenic region from Bacillus subtilis (107 aa), FASTA scores: opt: 272, E(): 5.3e-06, (39.4% identity in 104 aa overlap); P17577|YBAB_ECOLI|B0471|Z0588|ECS0524 from Escherichia coli strain K and O157:H7 (109 aa), FASTA scores: opt: 256, E(): 2.8e-05, (38.0% identity in 100 aa overlap); etc. Contains probable coiled-coil domain from aa 1-40. Seems to belong to the UPF0133 family.; hypothetical protein 4160913 885595 Rv3716c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218233.1 4160512 R 83332 CDS NP_218234.1 15610853 885602 4161048..4161773 1 NC_000962.3 Rv3717, (MTV025.065), len: 241 aa. Conserved hypothetical protein, equivalent to O69518|MLCB2407.19c (alias Q9CB75|ML2331 256 aa) hypothetical 25.1 KDA protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1325, E(): 5.7e-74, (81.95% identity in 244 aa overlap). Also similar to Q9KXK7|SCC53.04 putative secreted protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 536, E(): 1.2e-25, (41.2% identity in 233 aa overlap); and shows similarity with C-terminal end of other proteins e.g. Q9RMZ0|PXO2-42 PXO2-42 protein from Bacillus anthracis (531 aa), FASTA scores: opt: 191, E(): 0.00022, (26.6% identity in 222 aa overlap); Q9RTX0 putative N-acetylmuramoyl-L-alanine amidase (603 aa); Q9LCR4|CWLU CWLU protein from Paenibacillus polymyxa (Bacillus polymyxa) (524 aa), FASTA scores: opt: 141, E(): 0.24,(29.2% identity in 219 aa overlap); etc. Shows similarity with C-terminal end of O53593|CWLM|Rv3915|MTV028.06 putative hydrolase from Mycobacterium tuberculosis (406 aa), FASTA scores: opt: 176, E(): 0.0014, (25.7% identity in 218 aa overlap).; hypothetical protein 4161773 885602 Rv3717 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218234.1 4161048 D 83332 CDS NP_218235.1 15610854 885582 complement(4161815..4162258) 1 NC_000962.3 Rv3718c, (MTV025.066c), len: 147 aa. Conserved protein, equivalent to O69517|ML2332|MLCB2407.18 hypothetical 15.5 KDA protein from Mycobacterium leprae (145 aa), FASTA scores: opt: 780, E(): 1.4e-44, (81.95% identity in 144 aa overlap). Also highly similar to Q9ZBJ2|SC9C7.18 conserved hypothetical protein from Streptomyces coelicolor (147 aa) FASTA scores: opt: 475,E(): 1.7e-24, (52.05% identity in 146 aa overlap); and showing some similarity to various proteins e.g. P27538|PR2_PETCR pathogenesis-related protein 2 from Petroselinum crispum (Parsley) (Petroselinum hortense) (158 aa); P92918|ALL2_APIGR major allergen API G 2 from Apium graveolens (Celery) (159 aa); etc. Thought to be differentially expressed within host cells (see citation below).; hypothetical protein 4162258 885582 Rv3718c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218235.1 4161815 R 83332 CDS NP_218236.1 15610855 885855 4162306..4163718 1 NC_000962.3 Rv3719, (MTV025.067), len: 470 aa. Conserved protein, equivalent to O69516|ML2333|MLCB2407.17c hypothetical 51.8 KDA protein from Mycobacterium leprae (459 aa), FASTA scores: opt: 2593, E(): 7.8e-161, (82.75% identity in 458 aa overlap). Also some similarity to Q9CU63|5830417J06RIK hypothetical protein (fragment) from Mus musculus (Mouse) (479 aa) FASTA scores: opt: 454, E(): 6.1e-22, (27.1% identity in 413 aa overlap); Q9HBA8 seladin-1 (unknown) from Homo sapiens (Human) (516 aa),FASTA scores: opt: 444, E(): 2.9e-21, (26.7% identity in 412 aa overlap); O17397|DIMH_CAEEL|F52H2.6 diminuto-like protein from Caenorhabditis elegans (525 aa), FASTA scores: opt: 419, E(): 1.2e-19, (24.4% identity in 434 aa overlap); Q39085|DIM_ARATH|DWF1 cell elongation protein diminuto from Arabidopsis thaliana (Mouse-ear cress) (561 aa) FASTA scores: opt: 318, E(): 4.8e-13, (24.6% identity in 455 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis hypothetical proteins P72056|Rv3790|MTCY13D12.24 (461 aa) FASTA scores: opt: 174,E(): 0.00016; (25.1% identity in 426 aa overlap); and Q50685|Rv2280|MTCY339_30c (459 aa).; hypothetical protein 4163718 885855 Rv3719 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218236.1 4162306 D 83332 CDS NP_218237.3 448824818 885219 4163736..4164998 1 NC_000962.3 Rv3720, (MTV025.068), len: 420 aa. Possible fatty-acyl-phospholipid synthase, equivalent to Q9CB74|ML2334 (alias O69515|MLCB2407.16c, 439 aa) hypothetical protein from Mycobacterium leprae (420 aa) FASTA scores: opt: 2508, E(): 4.7e-153, (86.45% identity in 420 aa overlap). Also similar (especially at the C-terminus) to various fatty-acid synthases (principally cyclopropane-fatty-acyl-phospholipid synthases) and hypothetical proteins e.g. Q9KZ58|SCE25.32c putative fatty acid synthase from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1101, E(): 5.5e-63, (46.1% identity in 425 aa overlap); P31049|YLP3_PSEPU hypothetical 44.7 KDA protein from Pseudomonas putida (394 aa), FASTA scores: opt: 810,E(): 2.1e-44, (46.4% identity in 293 aa overlap); Q9HT28|PA5546 hypothetical protein from Pseudomonas aeruginosa (394 aa), FASTA scores: opt: 804, E(): 5.2e-44,(40.7% identity in 371 aa overlap); Q9RSD7|DR2187 putative cyclopropane-fatty-acyl-phospholipid synthase from Deinococcus radiodurans (462 aa), FASTA scores: opt: 747,E(): 2.6e-40, (35.95% identity in 409 aa overlap); BAB50831|Q98ET6|MLL4091 cyclopropane-fatty-acyl-phospholipid synthase from Rhizobium loti (Mesorhizobium loti) (422 aa), FASTA scores: opt: 674, E(): 1.1e-35, (39.1% identity in 284 aa overlap); P30010|CFA_ECOLI|CDFA|B1661 cyclopropane-fatty-acyl-phospholip synthase from Escherichia coli strain K12 (381 aa), FASTA scores: opt: 530, E(): 1.7e-26, (33.65% identity in 312 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis e.g. CMA2|Rv0503c|MTCY20G9.30c (302 aa); P96911|Rv0621|MTCY20H10 (354 aa); O50416|LPQD|Rv3390|MTV004.48 (236 aa); etc.; Possible fatty acid synthase 4164998 885219 Rv3720 Mycobacterium tuberculosis H37Rv Possible fatty acid synthase NP_218237.3 4163736 D 83332 CDS NP_218238.1 15610857 885361 complement(4164995..4166731) 1 NC_000962.3 Rv3721c, (MTV025.069c), len: 578 aa. Probable dnaZX,DNA polymerase III gamma (dnaZ) and tau (dnaX), equivalent to O69514|DNAZX|ML2335 DNA polymerase III subunit gamma/tau from Mycobacterium leprae (611 aa) FASTA scores: opt: 2344,E(): 4.7e-118, (78.75% identity in 602 aa overlap). Also highly similar to many e.g. Q9RKL5|DNAZ from Streptomyces coelicolor (784 aa) FASTA scores: opt: 1755, E(): 1.8e-86,(59.55% identity in 435 aa overlap); Q9KGM4|DNAX|BH0034 from Bacillus halodurans (564 aa), FASTA scores: opt: 946,E(): 2.5e-43, (37.4% identity in 460 aa overlap); P09122|DP3X_BACSU|DNAX|DNAH from Bacillus subtilis (563 aa), FASTA scores: opt: 841, E(): 1e-37, (30.8% identity in 510 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; DNA polymerase III (subunit gamma/tau) DnaZ/X 4166731 dnaZX 885361 dnaZX Mycobacterium tuberculosis H37Rv DNA polymerase III (subunit gamma/tau) DnaZ/X NP_218238.1 4164995 R 83332 CDS NP_218239.2 57117147 885321 complement(4166821..4168128) 1 NC_000962.3 Rv3722c, (MTV025.070c), len: 435 aa. Conserved protein, equivalent to O69513|MLCB2407.14 (alias Q9CB73|ML2336, 463 aa) hypothetical 46.8 KDA protein from Mycobacterium leprae (426 aa), FASTA scores: opt: 2505,E(): 8.3e-154, (87.25% identity in 424 aa overlap). Also highly similar to Q9RU17|DR1579 conserved hypothetical protein from Deinococcus radiodurans (452 aa), FASTA scores: opt: 1162, E(): 3.1e-67, (44.8% identity in 422 aa overlap); and partially similar to Q9I371|PA1654 probable aminotransferase from Pseudomonas aeruginosa (388 aa) FASTA scores: opt: 162, E(): 0.0078, (25.85% identity in 348 aa overlap) and other aminotransferases. N-terminus extended since first submission (previously 408 aa).; hypothetical protein 4168128 885321 Rv3722c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218239.2 4166821 R 83332 CDS NP_218240.1 15610859 885791 4168536..4169300 1 NC_000962.3 Rv3723, (MTV025.071), len: 254 aa. Probable conserved transmembrane protein, with hydrophobic stretches at the N-terminus, and equivalent to O69512|ML2337|MLCB2407.13c putative membrane protein from Mycobacterium leprae (250 aa), FASTA scores: opt: 1029,E(): 1.2e-44, (64.45% identity in 253 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 4169300 885791 Rv3723 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218240.1 4168536 D 83332 CDS YP_178007.1 57117148 3205112 4169467..4169709 1 NC_000962.3 Rv3724A, (MTV025.072), len: 80 aa. Probable cut5a,truncated cutinase precursor, similar to N-terminal end of others e.g. Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa), FASTA scores: opt: 202, E(): 1.5e-06, (56.45% identity in 62 aa overlap); Q9XB09|RVD2-RV1758 protein (fragment) from Mycobacterium bovis BCG (143 aa), FASTA scores: opt: 200, E(): 1.5e-06,(61.4% identity in 57 aa overlap); and Q00298|CUTI_BOTCI|CUTA cutinase precursor from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 108, E(): 2.2, (40.4% identity in 52 aa overlap). Also highly similar to others from Mycobacterium tuberculosis e.g. O06318|CUT3_MYCTU|Rv3451|MT3557|MTCY13E12.04 probable cutinase precursor (247 aa), FASTA scores: opt: 189, E(): 1.2e-05, (58.0% identity in 50 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 172, E(): 0.00015, (59.2% identity in 49 aa overlap); O06793|Rv1758|MTCY28.24|Z95890 hypothetical 17.9 KDA protein (174 aa), FASTA scores: opt: 641, E(): 2.7e-29,(57.2% identity in 166 aa overlap); O06319|Rv3452|MTY13E12.05; and U00015_11 from Mycobacterium leprae. Belongs to the cutinase family. Rest of cutinase ORF continues as Rv3724B|CUT5B, frameshifting could occur near position 4169668. Sequence has been checked but no errors found.; Probable cutinase precursor [first part] Cut5a 4169709 cut5a 3205112 cut5a Mycobacterium tuberculosis H37Rv Probable cutinase precursor [first part] Cut5a YP_178007.1 4169467 D 83332 CDS YP_178008.1 57117149 885390 4169606..4170169 1 NC_000962.3 Rv3724B, (MTV025.072), len: 187 aa. Probable cut5b,truncated cutinase, similar to C-terminal end of others e.g. Q9XB09|RVD2-RV1758 protein (fragment) from Mycobacterium bovis BCG (143 aa) FASTA scores: opt: 335,E(): 3.4e-12, (53.25% identity in 92 aa overlap); Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa),FASTA scores: opt: 251, E(): 2.5e-07, (44.05% identity in 168 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. O06793|Rv1758|MTCY28.24 hypothetical 17.9 KDA protein (174 aa), FASTA scores: opt: 641, E(): 2.5e-29, (57.25% identity in 166 aa overlap); O06319|Rv3452|MTCY13E12.05 hypothetical 23.1 KDA protein (226 aa), FASTA scores: opt: 385, E(): 7.5e-15, (46.65% identity in 165 aa overlap); O06318|CUT3_MYCTU|Rv3451|MT3557|MTCY13E12.04 probable cutinase precursor (247 aa), FASTA scores: opt: 307, E(): 1.9e-10, (40.7% identity in 167 aa overlap); Q10837|CUT1_MYCTU|Rv1984c|MT2037|MTCY39.35 probable cutinase precursor (217 aa), FASTA scores: opt: 261, E(): 6.7e-08, (50.9% identity in 169 aa overlap); etc; and U00015_11 from Mycobacterium lepra. 5'-end of gene is Rv3724A|CUT5A; frameshifting may occur near position 4169668.; Probable cutinase [second part] Cut5b 4170169 cut5b 885390 cut5b Mycobacterium tuberculosis H37Rv Probable cutinase [second part] Cut5b YP_178008.1 4169606 D 83332 CDS NP_218242.1 15610861 885887 4170214..4171143 1 NC_000962.3 Rv3725, (MTV025.073), len: 309 aa. Possible reductase, similar to various oxidoreductases and hypothetical proteins e.g. O34285|HPNA HPNA protein from Zymomonas mobilis (337 aa), FASTA scores: opt: 317, E(): 6.1e-11, (30.5% identity in 272 aa overlap); Q9SZB3|F17M5.120|AT4G33360|AAK49584 hypothetical 37.9 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (344 aa), FASTA scores: opt: 314, E(): 9.1e-11, (30.35% identity in 267 aa overlap); AAK59445|AT4G33360 putative dihydrokaempferol 4-reductase from Arabidopsis thaliana (Mouse-ear cress) (332 aa), FASTA scores: opt: 313, E(): 1e-10, (30.8% identity in 263 aa overlap); Q9FSC6|CCR cinnamoyl-CoA reductase from Populus trichocarpa (Western balsam poplar) (338 aa), FASTA scores: opt: 305, E(): 2.9e-10, (30.3% identity in 274 aa overlap); Q9M631 cinnamoyl CoA reductase from Populus tremuloides (Quaking aspen) (337 aa), FASTA scores: opt: 291, E(): 1.8e-09,(30.15% identity in 272 aa overlap); P73212|DFRA_SYNY3|LR1706 putative dihydroflavonol-4-reductase (dihydrokaempferol 4-reductase) from Synechocystis sp. strain PCC 6803 (343 aa), FASTA scores: opt: 278, E(): 1e-08, (29.35% identity in 259 aa overlap); etc. Also some similarity to proteins from Mycobacterium tuberculosis e.g. P96816|Rv0139|MTCI5.13 hypothetical protein (340 aa) FASTA scores: opt: 234, E(): 3.2e-06, (28.25% identity in 269 aa overlap); and O06373|galE1|Rv3634c|MTCY15C10.18 probable UDP-glucose 4-epimerase (314 aa) (27.3% identity in 194 aa overlap).; Possible oxidoreductase 4171143 885887 Rv3725 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_218242.1 4170214 D 83332 CDS NP_218243.1 15610862 885801 4171421..4172614 1 NC_000962.3 Rv3726, (MTV025.074), len: 397 aa. Possible dehydrogenase, similar to many e.g. O34788|YDJL dehydrogenase from Bacillus subtilis (346 aa) FASTA scores: opt: 401, E(): 3.4e-17, (29.6% identity in 395 aa overlap); Q59696|ADH 2,3-butanediol dehydrogenase from seudomonas putida (362 aa), FASTA scores: opt: 326, E(): 1.3e-12,(29.45% identity in 387 aa overlap); AAG59541|YJJN putative oxidoreductase from Escherichia coli strain EDL933 (345 aa), FASTA scores: opt: 325, E(): 1.5e-12, (30.85% identity in 256 aa overlap); Q9HWM8|PA4153 2,3-butanediol dehydrogenase from Pseudomonas aeruginosa (363 aa), FASTA scores: opt: 324, E(): 1.8e-12, (30.5% identity in 387 aa overlap); etc.; Possible dehydrogenase 4172614 885801 Rv3726 Mycobacterium tuberculosis H37Rv Possible dehydrogenase NP_218243.1 4171421 D 83332 CDS NP_218244.1 15610863 885766 4172955..4174763 1 NC_000962.3 Rv3727, (MTV025.075), len: 602 aa. Possible oxidoreductase, similar to several plants phytoene dehydrogenases/desaturases e.g. Q9HSE1|CRTI3|VNG0277G phytoene dehydrogenase from Halobacterium sp. strain NRC-1 (541 aa), FASTA scores: opt: 299, E(): 1.1e-10, (29.85% identity in 576 aa overlap); Q9FZL6|CITPDS1 phytoene desaturase from Citrus unshiu (Satsuma orange) (553 aa),FASTA scores: opt: 164, E(): 0.018, (24.2% identity in 434 aa overlap); Q07356|CRTI_ARATH|PDS|AT4G14210|DL3145c phytoene dehydrogenase precursor from Arabidopsis thaliana (Mouse-ear cress) (566 aa), FASTA scores: opt: 163, E(): 0.021, (23.95% identity in 434 aa overlap); etc. N-terminal end similar to O69871|SC1C3.29 putative protoporphyrinogen oxidase (fragment) from Streptomyces coelicolor (61 aa),FASTA scores: opt: 154, E(): 0.012, (60.45% identity in 43 aa overlap). The region between aa 155-310 is highly similar to Q49778|B2126_C1_169 from Mycobacterium leprae (159 aa), FASTA scores: opt: 437, E(): 1.5e-19, (46.6% identity in 161 aa overlap). And the region between aa 462-546 is highly similar to the N-terminal end of Q50003|U1764T from Mycobacterium leprae (155 aa), FASTA scores: opt: 277, E(): 8.3e-10, (57.65% identity in 85 aa overlap).; Possible oxidoreductase 4174763 885766 Rv3727 Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_218244.1 4172955 D 83332 CDS NP_218245.1 15610864 885271 4174873..4178070 1 NC_000962.3 Rv3728, (MTV025.076), len: 1065 aa. Probable conserved transmembrane protein organised into two domains. Domain comprising the first ~510 aa residues is similar to various multidrug resistance and efflux proteins and contains sugar transport protein signature 1 (PS00216). Domain corresponding to the last 550 aa residues contains cyclic nucleotide-binding domain signature 2 (PS00889) and is very similar to Q50733|YP65_MYCTU|Rv2565|MT2641|MTCY9C4.03c hypothetical 62.1 kDa protein from Mycobacterium tuberculosis (31.0% identity in 546 aa overlap). Highly similar to O05884|Rv3239c|MTCY20B11.14c probable transmembrane transport protein from Mycobacterium tuberculosis (1048 aa) FASTA scores: opt: 4328, E(): 5e-201, (64.15% identity in 1046 aa overlap). N-terminal end similar to P71879|Rv2333c|MTCY3G12.01|MTCY98.02c (537 aa); P71836|Rv0783c|MTCY369.27c (540 aa); and O07753|Rv1877|MTCY180.41c (687 aa). Seems belong to the sugar transporter family. Possibly member of major facilitator superfamily (MFS).; Probable conserved two-domain membrane protein 4178070 885271 Rv3728 Mycobacterium tuberculosis H37Rv Probable conserved two-domain membrane protein NP_218245.1 4174873 D 83332 CDS NP_218246.1 15610865 885706 4178285..4180615 1 NC_000962.3 Rv3729, (MTV025.077), len: 776 aa. Conserved hypothetical protein, possible transferase, similar to several hypothetical proteins and various transferases e.g. O26919|MTH831 molybdenum cofactor biosynthesis MOAA homolog from Methanobacterium thermoautotrophicum (497 aa), FASTA scores: opt: 697, E(): 4.8e-34, (30.7% identity in 492 aa overlap); Q58036|Y619_METJA|MJ0619 hypothetical protein from Methanococcus jannaschii (506 aa), FASTA scores: opt: 670, E(): 2e-32, (30.6% identity in 497 aa overlap); O27968|AF2316 conserved hypothetical protein from Archaeoglobus fulgidus (518 aa), FASTA scores: opt: 477,E(): 6.4e-21, (29.4% identity in 500 aa overlap); BAB60102|TVG0985801 molybdenum cofactor biosynthesis protein from Thermoplasma volcanium (606 aa), FASTA scores: opt: 402, E(): 2.1e-16, (28.1% identity in 509 aa overlap); etc. C-terminus similar to methyltransferases e.g. Q9S0N6|AVED C5-O-methyltransferase from Streptomyces avermitilis (283 aa), FASTA scores: opt: 298, E(): 1.9e-10,(31.5% identity in 292 aa overlap). Also similar to the Mycobacterium tuberculosis proteins P71673|YE05_MYCTU|Rv1405c|MT1449|MTCY21B4.22c (274 aa); and Q50584|Rv1523|MTCY19G5.05c.; Possible transferase 4180615 885706 Rv3729 Mycobacterium tuberculosis H37Rv Possible transferase NP_218246.1 4178285 D 83332 CDS NP_218247.1 15610866 885201 complement(4180680..4181720) 1 NC_000962.3 Rv3730c, (MTV025.078c), len: 346 aa. Conserved hypothetical protein, highly similar to Q9XAM1|SC4C6.19 hypothetical 38.5 KDA protein from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1313, E(): 2.2e-75, (59.25% identity in 336 aa overlap); and similar to C-terminal end of putative ATP-dependent DNA ligases e.g. BAB49297|MLL2077 from Rhizobium loti (Mesorhizobium loti) (833 aa), FASTA scores: opt: 550, E(): 5.3e-27, (31.3% identity in 294 aa overlap); and BAB54816|MLL9625 from Rhizobium loti (Mesorhizobium loti) plasmid pMLb (883 aa) FASTA scores: opt: 492, E(): 2.5e-23, (33.7% identity in 291 aa overlap); etc. Also similar to the hypothetical proteins e.g. Q9ZC15|SC1E6.07 hypothetical 34.9 KDA protein from Streptomyces coelicolor (319 aa) FASTA scores: opt: 537,E(): 1.5e-26, (34.95% identity in 292 aa overlap); Q9XAF7|SC6G9.25 hypothetical 32.1 KDA protein from Streptomyces coelicolor (293 aa), FASTA scores: opt: 474,E(): 1.3e-22, (33.75% identity in 302 aa overlap); etc. Also highly similar to P95226|Rv0269c|MTCY06A4.13c hypothetical 44.0 KDA protein from Mycobacterium tuberculosis (397 aa), FASTA scores: opt: 940, E(): 7.7e-52, (50.3% identity in 312 aa overlap).; hypothetical protein 4181720 885201 Rv3730c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218247.1 4180680 R 83332 CDS NP_218248.1 15610867 885771 4181758..4182834 1 NC_000962.3 Rv3731, (MTV025.079), len: 358 aa. Possible ligC,DNA ligase ATP-dependent (see citation below), similar to numerous archaebacterial and eukaryotic polynucleotide DNA ligases e.g. Q9XAM3|SC4C6.17c from Streptomyces coelicolor (355 aa), FASTA scores: opt: 1429, E(): 1.7e-82, (60.4% identity in 361 aa overlap); BAB54870|MLL9685 from Rhizobium loti (Mesorhizobium loti) plasmid pMLb (337 aa),FASTA scores: opt: 667, E(): 1.2e-34, (40.35% identity in 347 aa overlap); Q9HH07|DNLI_THEFM|LIG from Thermococcus fumicolans (559 aa), FASTA scores: opt: 335, E(): 1.4e-13,(27.25% identity in 330 aa overlap); O59288|DNLI_PYRHO from Pyrococcus horikoshii (559 aa), FASTA scores: opt: 307,E(): 8e-12, (26.85% identity in 272 aa overlap); etc. Also similar to Rv3062|MTCY22D7_19c|LIGB probable DNA ligase from Mycobacterium tuberculosis (507 aa), FASTA score: (30.3% identity in 356 aa overlap). Seems to belong to the ATP-dependent DNA ligase family.; Possible ATP-dependent DNA ligase LigC (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase) 4182834 ligC 885771 ligC Mycobacterium tuberculosis H37Rv Possible ATP-dependent DNA ligase LigC (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase) NP_218248.1 4181758 D 83332 CDS NP_218249.1 15610868 885795 4182934..4183992 1 NC_000962.3 Rv3732, (MTV025.080), len: 352 aa. Conserved protein. The region between aa 175-352 is highly similar to the region between aa 72-257 of Q9KH39 hypothetical 55.5 KDA protein from Mycobacterium smegmatis (511 aa), FASTA scores: opt: 1122, E(): 7.3e-63, (98.85% identity in 176 aa overlap). Also shows some similarity with Q55304 hypotheticalk protein from Synechocystis sp. strain PCC 6803 (387 aa), FASTA scores: opt: 201, E(): 2.7e-05, (27.1% identity in 251 aa overlap); and P74254|SLR1173 hypothetical 52.5 KDA protein from Synechocystis sp. strain PCC 6803 (463 aa), FASTA scores: opt: 201, E(): 3.1e-05,(27.1% identity in 251 aa overlap). Also slightly similar to MTCY01B2_21 and DPO1_MYCTU DNA polymerase I.; hypothetical protein 4183992 885795 Rv3732 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218249.1 4182934 D 83332 CDS NP_218250.1 15610869 885721 complement(4184012..4184512) 1 NC_000962.3 Rv3733c, (MTV025.081c), len: 166 aa. Conserved hypothetical protein, highly similar to Q9FCB0|2SCG58.03 putative mutt-like protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 541, E(): 7.2e-29, (52.7% identity in 148 aa overlap); and BAB49143|MLR1881 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (156 aa), FASTA scores: opt: 526, E(): 7.2e-28,(52.65% identity in 150 aa overlap).; hypothetical protein 4184512 885721 Rv3733c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218250.1 4184012 R 83332 CDS NP_218251.1 15610870 885335 complement(4184526..4185890) 1 NC_000962.3 Rv3734c, (MTV025.082c), len: 454 aa. Putative tgs2,triacylglycerol synthase (See Daniel et al., 2004), highly similar to O69707|Y1E0_MYCTU|Rv3740c|MT3848|MTV025.088c hypothetical protein from Mycobacterium tuberculosis (448 aa), FASTA scores: opt: 1917, E(): 1.3e-111, (61.4% identity in 451 aa overlap); and similar to many other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. P71694|YE43_MYCTU|Rv1425|MT1468|MTCY21B4.43|MTCY493.29c (459 aa), FASTA scores: opt: 824, E(): 1.1e-43, (36.5% identity in 460 aa overlap); Q50680|YM85_MYCTU|Rv2285|MT2343|MTCY339.25c (445 aa) FASTA scores: opt: 766, E(): 4.1e-40, (36.4% identity in 453 aa overlap); etc. Also similar to Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 331, E(): 4.3e-13, (32.9% identity in 468 aa overlap); and Q9X7A8|ML1244|MLCB1610.05 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 296, E(): 7e-11, (28.35% identity in 413 aa overlap). Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. Start site chosen by homology, but may extend further upstream to 93257.; Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs2 4185890 tgs2 885335 tgs2 Mycobacterium tuberculosis H37Rv Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs2 NP_218251.1 4184526 R 83332 CDS NP_218252.1 15610871 885318 4186089..4186577 1 NC_000962.3 Rv3735, (MTV025.083), len: 162 aa. Conserved hypothetical protein, highly similar to several bacterial hypothetical proteins e.g. Q9UX41|ORF-C09_016|SSO0651|AAK40956 from Sulfolobus solfataricus (163 aa), FASTA scores: opt: 627, E(): 1.2e-34, (55.9% identity in 161 aa overlap); O26795|MTH699 from Methanobacterium thermoautotrophicum (168 aa), FASTA scores: opt: 616, E(): 6.7e-34, (56.1% identity in 155 aa overlap); |Q9Y9J9|APE2289 from Aeropyrum pernix (191 aa),FASTA scores: opt: 591, E(): 3.4e-32, (54.65% identity in 161 aa overlap) ; etc. Contains PS00435 Peroxidases proximal heme-ligand signature.; hypothetical protein 4186577 885318 Rv3735 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218252.1 4186089 D 83332 CDS NP_218253.1 15610872 885389 4186634..4187695 1 NC_000962.3 Rv3736, (MTV025.084), len: 353 aa. Probable transcriptional regulator, araC/xylS family, similar to many transcriptional regulators and hypothetical proteins e.g. CAC38740 hypothetical 35.4 KDA protein from Bradyrhizobium japonicum (318 aa), FASTA scores: opt: 438,E(): 2e-20, (29.4% identity in 306 aa overlap); Q9HZ25|PA3215 probable transcriptional regulator from Pseudomonas aeruginosa (337 aa), FASTA scores: opt: 395,E(): 1.1e-17, (30.3% identity in 320 aa overlap); Q9HTN1|PA5324 probable transcriptional regulator from Pseudomonas aeruginosa (356 aa), FASTA scores: opt: 313,E(): 1.8e-12, (25.85% identity in 329 aa overlap); Q9Z3Y6|PHBR transcriptional regulator PHBR from Pseudomonas sp. 61-3 (379 aa), FASTA scores: opt: 271, E(): 8.3e-10,(22.95% identity in 357 aa overlap); etc. Also highly similar to Q06861|VIRS_MYCTU|Rv3082c|MTV013.03c possible virulence-regulating protein from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 656, E(): 3.7e-34, (36.95% identity in 333 aa overlap); and similar to other hypothetical mycobacterial proteins e.g. P71663|YD95_MYCTU|Rv1395|MT1440|MTCY21B4.12 (344 aa). Contains helix-turn-helix motif at aa 245-266 (Score 1140,+3.07 SD). Seems belong to the AraC/XylS family of transcriptional regulators.; Transcriptional regulatory protein (probably AraC/XylS-family) 4187695 885389 Rv3736 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein (probably AraC/XylS-family) NP_218253.1 4186634 D 83332 CDS NP_218254.1 15610873 885794 4187699..4189288 1 NC_000962.3 Rv3737, (MTV025.085), len: 529 aa. Probable conserved transmembrane protein, similar to others and also some hypothetical proteins e.g. AAK61331|THRE threonine export carrier from Corynebacterium glutamicum (Brevibacterium flavum) (489 aa), FASTA scores: opt: 773,E(): 1.8e-36, (37.25% identity in 424 aa overlap); Q9X8J0|SCE9.17 putative membrane protein from Streptomyces coelicolor (578 aa), FASTA scores: opt: 642, E(): 5.4e-29,(31.6% identity in 481 aa overlap) (shorter 119 aa at N-terminus); Q9CJU6|PM1895 hypothetical protein from Pasteurella multocida (262 aa), FASTA scores: opt: 233,E(): 4.1e-06, (25.0% identity in 256 aa overlap); Q9S267|SCI30A.06 putative integral membrane protein from Streptomyces coelicolor (297 aa), FASTA scores: opt: 163,E(): 0.042, (29.65% identity in 263 aa overlap); etc. Also partially similar to O05435|Rv3910|MTCY15F10.01c|MTV028.01 hypothetical 123.6 KDA protein from Mycobacterium tuberculosis (1184 aa) (34.4% identity in 125 aa overlap).; Probable conserved transmembrane protein 4189288 885794 Rv3737 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218254.1 4187699 D 83332 CDS YP_178009.1 57117150 886262 complement(4189285..4190232) 1 NC_000962.3 Rv3738c, (MTV025.086c), len: 315 aa. PPE66, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O53265|Rv3018c|MTV012.32c (434 aa),FASTA scores: opt: 464, E(): 2.2e-17, (47.05% identity in 338 aa overlap). Probably a continuation of the upstream ORF MTV025.87c|Rv3739c|PPE67. At position 97470-72 a stop codon is present which interrupts a possibly longer ORF,observed in related ORFs MTV012_32 or MTCY21B4_4. The sequence has been checked and no errors were detected. A similar situation, but with a frameshift separating the ORFs is found in MTV012_36/MTV012_35. Sequence similarity is also seen with MTCY251_15; MTCY261_19; MLCB2492_30 from Mycobacterium leprae; MTCY10G2_10; MTY21C12_9; MTCI125_26; MTCY164_36; MTCY6A4_1.; PPE family protein PPE66 4190232 PPE66 886262 PPE66 Mycobacterium tuberculosis H37Rv PPE family protein PPE66 YP_178009.1 4189285 R 83332 CDS YP_178010.1 57117151 886257 complement(4190284..4190517) 1 NC_000962.3 Rv3739c, (MTV025.087c), len: 77 aa. PPE67, Member of the Mycobacterium tuberculosis PE family, showing high homology with O53269|Rv3022c|MTV012.36c (82 aa) FASTA scores: opt: 398, E(): 1.2e-19, (74.0% identity in 77 aa overlap); and similar to the N-termini of other PPE proteins e.g. O53265|Rv3018c|MTV012.32c (434 aa) FASTA scores: opt: 398, E(): 4.8e-19, (74.0% identity in 77 aa overlap). ORF ends at the stop codon at position 97470,which is not present in similar ORFs: MTV012_32, or MTCY21B4_4. Sequence homology with MTV012_32, and MTCY21B4_4 continues in the downstream ORF MTV025.086c|Rv3738c|PPE66. Sequence was checked, but no errors were detected. A similar situation, but with a frameshift separating the ORFs, is found in MTV012_36/MTV012_35. Also ORF MTV025.87c shows similarity to MTV03 _14; MTCY6A4_1; MTV035_8; MTV037_17; MLCB2492_30; MTCY261_19; MTCY251_15; MTCY3A2_23; MTCY28_16; etc.; PPE family protein PPE67 4190517 PPE67 886257 PPE67 Mycobacterium tuberculosis H37Rv PPE family protein PPE67 YP_178010.1 4190284 R 83332 CDS NP_218257.1 15610876 885781 complement(4190833..4192179) 1 NC_000962.3 Rv3740c, (MTV025.088c), len: 448 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), highly similar to several other Mycobacterium tuberculosis hypothetical proteins e.g. O69701|Y1D4_MYCTU|Rv3734c|MT3839|MTV025.082c (454 aa) FASTA scores: opt: 1917, E(): 2.3e-112, (61.4% identity in 451 aa overlap); Q50680|YM85_MYCTU|Rv2285|MT2343|MTCY339.25c (445 aa) FASTA scores: opt: 858, E(): 3.4e-46, (37.4% identity in 460 aa overlap); Q10554|Y895_MYCTU|Rv0895|MT0919|MTCY31.23 (505 aa), FASTA scores: opt: 767, E(): 1.9e-40, (44.3% identity in 467 aa overlap); MTCY31_25; MTCY28_26; MTCY493_29; MTCY21B4_43; MTCY8D5_16; MTCY3A2_28; MTV013_8; MTY13E12_33; MTV013_9; MTY20B11_9; etc. Also similar to Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 319, E(): 1.7e-12, (30.9% identity in 453 aa overlap).; Possible triacylglycerol synthase (diacylglycerol acyltransferase) 4192179 885781 Rv3740c Mycobacterium tuberculosis H37Rv Possible triacylglycerol synthase (diacylglycerol acyltransferase) NP_218257.1 4190833 R 83332 CDS NP_218258.1 15610877 885129 complement(4192179..4192853) 1 NC_000962.3 Rv3741c, (MTV025.089c), len: 224 aa. Possible oxidoreductase, probably combines with product of upstream ORF MTV025.090c to form a functional monooxygenase, highly similar to C-terminal end of various oxidoreductases e.g. Q9APW3 aromatic-ring hyroxylase from Pseudomonas aeruginosa (508 aa), FASTA scores: opt: 549, E(): 5.9e-28, (56.1% identity in 155 aa overlap); Q9A588|CC2569 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa), FASTA scores: opt: 487, E(): 5.6e-24, (39.55% identity in 225 aa overlap); Q9RZT0|DRB0033 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa), FASTA scores: opt: 460, E(): 4.7e-22, (38.5% identity in 226 aa overlap); etc. Also similar to C-terminal end of Mycobacterium tuberculosis proteins (generally monooxygenases) e.g. P96223|Rv3854c|MTCY01A6.14 hypothetical 55.3 KDA protein (489 aa), FASTA scores: opt: 542, E(): 1.6e-27, (50.0% identity in 162 aa overlap); O53762|Rv0565c|MTV039.03c putative monoxygenase (486 aa),FASTA scores: opt: 462, E(): 2.2e-22, (37.15% identity in 226 aa overlap); O53300|Rv3083|MTV013.04 monoxygenase (495 aa), FASTA scores: opt: 462, E(): 2.2e-22, (45.65% identity in 173 aa overlap); etc. Note similarity to MTCY01A6.14 and MTV013.04 continue in upstream ORF (MTV025.090c) after a gap of ~100 aa.; Possible oxidoreductase 4192853 885129 Rv3741c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_218258.1 4192179 R 83332 CDS NP_218259.1 15610878 885263 complement(4192850..4193245) 1 NC_000962.3 Rv3742c, (MTV025.090c), len: 131 aa. Possible oxidoreductase, probably combines with product of downstream ORF MTV025.090c to form a functional monooxygenase, highly similar to N-terminal end of various oxidoreductases e.g. Q9A588|CC2569 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa), FASTA scores: opt: 170, E(): 0.00048, (47.55% identity in 103 aa overlap); Q9APW3 aromatic-ring hyroxylase from Pseudomonas aeruginosa (508 aa) FASTA scores: opt: 160,E(): 0.0022, (50.55% identity in 87 aa overlap); Q9RZT0|DRB0033 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa), FASTA scores: opt: 153, E(): 0.0097,(45.45% identity in 88 aa overlap); etc. Also similar to C-terminal end of Mycobacterium tuberculosis proteins (generally monooxygenases) e.g. P96223|Rv3854c|MTCY01A6.14 hypothetical 55.3 KDA protein (489 aa), FASTA scores: opt: 140, E(): 0.044, (37.1% identity in 132 aa overlap); O53300|Rv3083|MTV013.04 monoxygenase (495 aa) FASTA scores: opt: 133, E(): 0.13, (43.05% identity in 79 aa overlap); O53762|Rv0565c|MTV039.03c putative monoxygenase (486 aa),FASTA scores: opt: 110, E(): 4.1, (42.85% identity in 77 aa overlap); etc. Note similarity to MTCY01A6.14 and MTV013.04 continue in downstream ORF (MTV025.089c) after a gap of ~100 aa.; Possible oxidoreductase 4193245 885263 Rv3742c Mycobacterium tuberculosis H37Rv Possible oxidoreductase NP_218259.1 4192850 R 83332 CDS NP_218260.1 15610879 885106 complement(4193391..4195373) 1 NC_000962.3 Rv3743c, (MTV025.091c), len: 660 aa. Probable ctpJ,cation-transporting P-type ATPase, transmembrane protein highly similar to others e.g. Q9ZBF3|SC9B5.27 putative cation-transporting ATPase from Streptomyces coelicolor (638 aa), FASTA scores: opt: 1635, E(): 2.5e-86, (62.25% identity in 63.95 aa overlap); Q59997|CADA|SLR0797 cadmium-transporting ATPase from Synechocystis sp. strain PCC 6803 (642 aa), FASTA scores: opt: 1474, E(): 4.3e-77,(42.4% identity in 604 aa overlap); P30336|CADA_BACFI probable cadmium-transporting ATPase from Bacillus firmus (723 aa), FASTA scores: opt: 1327, E(): 1.3e-68, (36.6% identity in 626 aa overlap); etc. Also highly similar to O53160|CTPD_MYCTU|Rv1469|MT1515|MTV007.16 probable cation-transporting P-type ATPase D from Mycobacterium tuberculosis (657 aa), FASTA scores: opt: 1845, E(): 2.3e-98, (55.85% identity in 650 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site and PS01229 Hypothetical family signature 2. Belongs to the cation transport ATPases family (E1-E2 ATPases). Transcription is repressed by NmtR (See Cavet et al., 2002).; Probable cation transporter P-type ATPase CtpJ 4195373 ctpJ 885106 ctpJ Mycobacterium tuberculosis H37Rv Probable cation transporter P-type ATPase CtpJ NP_218260.1 4193391 R 83332 CDS NP_218261.1 15610880 885418 4195440..4195802 1 NC_000962.3 Rv3744, (MTV025.092), len: 120 aa. Transcriptional regulator nmtR (See Cavet et al., 2002). Highly similar to many e.g. Q9ZBF4|SC9B5.26c from Streptomyces coelicolor (120 aa), FASTA scores: opt: 480, E(): 2.4e-24, (63.25% identity in 117 aa overlap); O31844|YOZA YOZA regulator from Bacillus subtilis (107 aa), FASTA scores: opt: 249,E(): 1.6e-09, (44.8% identity in 96 aa overlap); P30340|SMTB_SYNP7|SMTB from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (122 aa), FASTA scores: opt: 230, E(): 2.9e-08, (46.0% identity in 87 aa overlap); etc. Equivalent to AAK48216 from Mycobacterium tuberculosis strain CDC1551 (135 aa) but shorter 15 aa. Also similar to MTCY27_22; MTCY39_25; and MTCY441_12. Contains helix-turn-helix motif at aa 47-68 (Score 1815, +5.37 SD). Belongs to the ArsR-SmtB family of transcriptional regulators.; Metal sensor transcriptional regulator (ArsR-SmtB family) 4195802 nmtR 885418 nmtR Mycobacterium tuberculosis H37Rv Metal sensor transcriptional regulator (ArsR-SmtB family) NP_218261.1 4195440 D 83332 CDS NP_218262.1 15610881 885597 complement(4195886..4196098) 1 NC_000962.3 Rv3745c, (MTV025.093c), len: 70 aa. Conserved hypothetical protein, highly similar to others e.g. N-terminus of Q9X4E6 hypothetical 13.4 KDA protein from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (124 aa), FASTA scores: opt: 279, E(): 4.4e-14, (59.4% identity in 69 aa overlap); N-terminus of Q9A2A6|CC3660 hypothetical protein from Caulobacter crescentus (172 aa) FASTA scores: opt: 272, E(): 1.9e-13, (63.35% identity in 60 aa overlap); N-terminus of P74345|SLR1628 hypothetical 14.5 KDA protein from Synechocystis sp. strain PCC 6803 (134 aa), FASTA scores: opt: 233, E(): 1.3e-10, (54.85% identity in 62 aa overlap); etc.; hypothetical protein 4196098 885597 Rv3745c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218262.1 4195886 R 83332 CDS YP_178011.1 57117152 885764 complement(4196171..4196506) 1 NC_000962.3 Rv3746c, (MTV025.094c), len: 111 aa. PE34, Probable member of the Mycobacterium tuberculosis PE family (see citation below), but without the glycine-rich C-terminal part, similar to N-termini of many e.g. O69737|Rv3872|MTV027.07 (99 aa) FASTA scores: opt: 306,E(): 1e-13, (50.5% identity in 99 aa overlap); O53215|Rv2490c|MTV008.46 (1660 aa) FASTA scores: opt: 125,E(): 0.99, (34.25% identity in 111 aa overlap). Also weakly similar to MTV008_46; MTCI418B_6; MTCY130_1; MTY25D10_11; MTCY1A11_25; MTCY21B4_13; MTCY21B4_27; MTCY493_2; MTCY28_25; etc.; Probable PE family protein PE34 (PE family-related protein) 4196506 PE34 885764 PE34 Mycobacterium tuberculosis H37Rv Probable PE family protein PE34 (PE family-related protein) YP_178011.1 4196171 R 83332 CDS NP_218264.1 15610883 885778 4196724..4197107 1 NC_000962.3 Rv3747, (MTV025.095), len: 127 aa. Conserved protein, highly similar to downstream ORF O69715|Rv3748|MTV025.096 conserved hypothetical protein (119 aa), FASTA scores: opt: 494, E(): 6e-27, (64.4% identity in 118 aa overlap).; hypothetical protein 4197107 885778 Rv3747 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218264.1 4196724 D 83332 CDS NP_218265.1 15610884 885776 4197236..4197595 1 NC_000962.3 Rv3748, (MTV025.096), len: 119 aa. Hypothetical protein, highly similar to upstream ORF O69714|Rv3747|MTV025.095 conserved hypothetical protein (127 aa), FASTA scores: opt: 496, E(): 2.5e-28, (64.4% identity in 118 aa overlap).; hypothetical protein 4197595 885776 Rv3748 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218265.1 4197236 D 83332 CDS NP_218266.1 15610885 885384 complement(4197628..4198137) 1 NC_000962.3 Rv3749c, (MTV025.097c), len: 169 aa. Hypothetical protein, showing some similarity with O85864 hypothetical 21.4 KDA protein from Sphingomonas aromaticivorans plasmid pNL1 (196 aa), FASTA scores: opt: 148, E(): 0.011, (32.7% identity in 104 aa overlap); Q9LCU6 hypothetical 21.2 KDA protein from Arthrobacter sp. TM1 (192 aa), FASTA scores: opt: 125, E(): 0.35, (31.5% identity in 92 aa overlap); Q9L631|SPCB myo-inositol-2-dehydrogenase from Streptomyces spectabilis (374 aa); Q9WJP8|PRE-S1 PRE-S1 protein (fragment) from Hepatitis B virus (88 aa); etc. Contains PS00092 N-6 Adenine-specific DNA methylases signature. Predicted to be an outer membrane protein (See Song et al.,2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; hypothetical protein 4198137 885384 Rv3749c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218266.1 4197628 R 83332 CDS NP_218267.1 15610886 885807 complement(4198205..4198597) 1 NC_000962.3 Rv3750c, (MTV025.098c), len: 130 aa. Possible excisionase, similar to others e.g. Q9LCU5 putative excisionase from Arthrobacter sp. TM1 (174 aa) FASTA scores: opt: 297, E(): 1.2e-12, (40.35% identity in 114 aa overlap); O85865 putative excisionase from Sphingomonas aromaticivorans plasmid pNL1 (152 aa), FASTA scores: opt: 223, E(): 7.3e-08, (39.15% identity in 97 aa overlap); Q9XBH1|xis excisionase from Bacteroides fragilis (124 aa) FASTA scores: opt: 128, E(): 0.1, (30.7% identity in 88 aa overlap); etc. Also some similarity to transcriptional regulators. Also similar to Mycobacterium tuberculosis hypothetical proteins e.g. P71902|YN10_MYCTU|Rv2310|MT2372|MTCY3G12.24c (114 aa) FASTA scores: opt: 224, E(): 4.9e-08, (42.7% identity in 82 aa overlap). Contains helix-turn-helix motif at aa 55-76 (Score 1925,+5.74 SD). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Possible excisionase 4198597 885807 Rv3750c Mycobacterium tuberculosis H37Rv Possible excisionase NP_218267.1 4198205 R 83332 CDS NP_218268.1 15610887 885857 4198874..4199089 1 NC_000962.3 Rv3751, (MTV025.099), len: 71 aa. Probable integrase (fragment), similar to part of many e.g. Q48908 integrase (fragment) from Mycobacterium paratuberculosis (191 aa),FASTA scores: opt: 206, E(): 5.5e-08, (57.65% identity in 59 aa overlap); Q9ZWV7|int integrase from Corynephage 304L (395 aa), FASTA scores: opt: 156, E(): 0.00036, (45.75% identity in 59 aa overlap); Q9K722|BH3551 integrase (phage-related protein) from Bacillus halodurans (378 aa),FASTA scores: opt: 151, E(): 0.00079, (46.15% identity in 52 aa overlap); etc. Also similarity with various conjugative transposons. Also similar to Mycobacterium tuberculosis hypothetical proteins e.g. P71903|Rv2309c|MTCY3G12.25 (151 aa), FASTA scores: opt: 193, E(): 3.8e-07, (50.85% identity in 59 aa overlap); O53403|Rv1055|MTV017.08 (78 aa), FASTA scores: opt: 171,E(): 7.8e-06, (54.15% identity in 48 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Probable integrase (fragment) 4199089 885857 Rv3751 Mycobacterium tuberculosis H37Rv Probable integrase (fragment) NP_218268.1 4198874 D 83332 CDS NP_218269.1 15610888 885586 complement(4199247..4199705) 1 NC_000962.3 Rv3752c, (MTV025.100c), len: 152 aa. Probable cytidine/deoxycytidylate deaminase, equivalent to Q9CB32|ML2474 possible cytidine/deoxycytidylate deaminase from Mycobacterium leprae (171 aa), FASTA scores: opt: 890,E(): 1.6e-50, (88.1% identity in 151 aa overlap). Also highly similar to other deaminases and hypothetical proteins e.g. Q9AK79|2SCD60.04c putative deaminase from Streptomyces coelicolor (143 aa), FASTA scores: opt: 559,E(): 2.9e-29, (66.45% identity in 146 aa overlap); Q9F9W7 cytosine deaminase from Bifidobacterium longum (143 aa) FASTA scores: opt: 512, E(): 3.1e-26, (54.85% identity in 144 aa overlap); P21335|YAAJ_BACSU hypothetical 17.8 KDA protein from Bacillus subtilis (161 aa), FASTA scores: opt: 425, E(): 1.4e-20, (47.7% identity in 151 aa overlap); AAK74212|SP0020 cytidine/deoxycytidylate deaminase family protein from Streptococcus pneumoniae (155 aa), FASTA scores: opt: 401, E(): 4.7e-19, (46.25% identity in 147 aa overlap); P30134|YFHC_ECOLI|B2559 hypothetical 20.0 KDA protein from Escherichia coli strain K12 (178 aa), FASTA scores: opt: 397, E(): 9.5e-19, (47.0% identity in 149 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family.; Possible cytidine/deoxycytidylate deaminase 4199705 885586 Rv3752c Mycobacterium tuberculosis H37Rv Possible cytidine/deoxycytidylate deaminase NP_218269.1 4199247 R 83332 CDS NP_218270.3 448824819 885505 complement(4199721..4200221) 1 NC_000962.3 Rv3753c, (MTV025.101c), len: 166 aa. Conserved protein, only equivalent to Q9CB33|ML2473 hypothetical protein from Mycobacterium leprae (159 aa) FASTA scores: opt: 920 E(): 1.4e-52,, (88.6% identity in 158 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 4200221 885505 Rv3753c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218270.3 4199721 R 83332 CDS NP_218271.3 448824820 885559 4200421..4201326 1 NC_000962.3 Rv3754, (MTV025.102), len: 301 aa. Probable tyrA,prephenate dehydrogenase, equivalent, but shorter 27 aa, to Q9CB34|ML2472 possible prephenate dehydrogenase from Mycobacterium leprae (327 aa) FASTA scores: opt: 1600, E(): 1.6e-89, (80.0% identity in 300 aa overlap). Also similar to many pephenate dehydrogenases e.g. Q9RND8|TYRA from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (299 aa), FASTA scores: opt: 345, E(): 9.7e-14, (32.85% identity in 271 aa overlap); Q9RVA7|DR1122 from Deinococcus radiodurans (372 aa) FASTA scores: opt: 341, E(): 2e-13,(35.65% identity in 216 aa overlap); P20692|TYRA_BACSU from Bacillus subtilis (372 aa), FASTA scores: opt: 314, E(): 8.6e-12, (27.75% identity in 263 aa overlap); etc. Also similar to Q04983|TYRC_ZYMMO TYRC protein [includes: cyclohexadienyl dehydrogenase and prephenate dehydrogenase activities] from Zymomonas mobilis (293 aa), FASTA scores: opt: 290, E(): 2e-10, (30.15% identity in 239 aa overlap). Equivalent to AAK48225 from Mycobacterium tuberculosis strain CDC1551 (323 aa) but shorter 22 aa.; Prephenate dehydrogenase TyrA (PDH) (hydroxyphenylpyruvate synthase) 4201326 tyrA 885559 tyrA Mycobacterium tuberculosis H37Rv Prephenate dehydrogenase TyrA (PDH) (hydroxyphenylpyruvate synthase) NP_218271.3 4200421 D 83332 CDS NP_218272.1 15610891 885298 complement(4201289..4201888) 1 NC_000962.3 Rv3755c, (MTV025.103c), len: 199 aa. Conserved protein showing similarity to CAC47343|SMC03980 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (196 aa) FASTA scores: opt: 244, E(): 4.1e-09,(30.9% identity in 191 aa overlap); Q9I2B5|PA1994 from Pseudomonas aeruginosa (187 aa), FASTA scores: opt: 226,E(): 6e-08, (29.9% identity in 194 aa overlap); and Q98N73|MLR0268 hypothetical protein (183 aa), FASTA scores: opt: 234, E(): 1.8e-08, (27.05% identity in 185 aa overlap).; hypothetical protein 4201888 885298 Rv3755c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218272.1 4201289 R 83332 CDS NP_218273.1 15610892 885534 complement(4201894..4202613) 1 NC_000962.3 Rv3756c, (MTV025.104c), len: 239 aa. Possible proZ,osmoprotectant transport integral membrane protein ABC transporter (see citation below), similar to osmoprotection proteins (proW, proZ) involved in glycine betaine/L-proline/choline transport, e.g. BAB58609|Q99RI4|OPUCB|SA2236|SAV2447 OPUCB protein (probable glycine betaine/carnitine/choline ABC transporter) from Staphylococcus aureus (211 aa) FASTA scores: opt: 434, E(): 2.5e-18, (36.6% identity in 194 aa overlap); Q45461|OPBB_BACSU|OPUBB|prow choline transport system permease protein (mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine) from Bacillus subtilis (217 aa), FASTA scores: opt: 402, E(): 1.9e-16, (32.0% identity in 203 aa overlap); O34878|OPCB_BACSU|OPUCB glycine betaine/carnitine/choline transport system permease protein from Bacillus subtilis (217 aa), FASTA scores: opt: 385, E(): 1.8e-15, (30.2% identity in 222 aa overlap); P39775|O34657|OPUBD|PROZ|OPBD_BACSU choline transport system permease protein from Bacillus subtilis (226 aa) FASTA scores: opt: 350, E(): 2e-13, (31.75% identity in 208 aa overlap); etc. Could belong to the CYSTW subfamily.; Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProZ 4202613 proZ 885534 proZ Mycobacterium tuberculosis H37Rv Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProZ NP_218273.1 4201894 R 83332 CDS NP_218274.1 15610893 885581 complement(4202610..4203299) 1 NC_000962.3 Rv3757c, (MTV025.105c), len: 229 aa. Possible proW,osmoprotectant transport integral membrane protein ABC transporter (see citation below), similar to osmoprotection proteins (proW, proZ) involved in glycine betaine/L-proline/choline transport, e.g. BAB58607|Q99RI6|OPUCD|SA2234|SAV2445 OPUCD protein (probable glycine betaine/carnitine/choline ABC transporter) from Staphylococcus aureus (231 aa) FASTA scores: opt: 364, E(): 7.1e-15, (30.0% identity in 220 aa overlap); Q45461|OPBB_BACSU|OPUBB|prow choline transport system permease protein (mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine) from Bacillus subtilis (217 aa), FASTA scores: opt: 348, E(): 6.2e-14, (31.05% identity in 206 aa overlap); O34878|OPCB_BACSU|OPUCB glycine betaine/carnitine/choline transport system permease protein from Bacillus subtilis (217 aa), FASTA scores: opt: 343, E(): 1.2e-13, (30.1% identity in 206 aa overlap); O34742|OPCD_BACSU|OPUCD glycine betaine/carnitine/choline transport system permease protein from Bacillus subtilis (229 aa) FASTA scores: opt: 337, E(): 2.9e-13, (31.1% identity in 193 aa overlap); etc. Could belong to the CYSTW subfamily.; Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProW 4203299 proW 885581 proW Mycobacterium tuberculosis H37Rv Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProW NP_218274.1 4202610 R 83332 CDS NP_218275.1 15610894 886293 complement(4203287..4204417) 1 NC_000962.3 Rv3758c, (MTV025.106c), len: 376 aa. Possible proV,osmoprotectant transport ATP-binding protein ABC transporter (see citation below), highly similar to osmoprotection proteins (proV) involved in glycine betaine/L-proline/choline transport, e.g. BAB58610|Q99RI3|OPUCA|SA2237|SAV2448 glycine betaine/carnitine/choline ABC transporter (ATP-binding) from Staphylococcus aureus (410 aa), FASTA scores: opt: 816, E(): 8.4e-39, (39.5% identity in 362 aa overlap); O34992|OPCA_BACSU|OPUCA glycine betaine/carnitine/choline transport ATP-binding protein from Bacillus subtilis (380 aa), FASTA scores: opt: 807, E(): 2.5e-38, (40.55% identity in 333 aa overlap); Q45460|OPBA_BACSU|OPUBA|prov choline transport ATP-binding protein from Bacillus subtilis (381 aa), FASTA scores: opt: 801, E(): 5.6e-38, (40.65% identity in 337 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporter family signature. Belongs to the ATP-binding transport protein family (ABC transporters).; Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport ATP-binding protein ABC transporter ProV 4204417 proV 886293 proV Mycobacterium tuberculosis H37Rv Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport ATP-binding protein ABC transporter ProV NP_218275.1 4203287 R 83332 CDS NP_218276.1 15610895 886270 complement(4204426..4205373) 1 NC_000962.3 Rv3759c, (MTV025.107c), len: 315 aa. Possible proX,osmoprotectant-binding lipoprotein component of osmoprotectant transport system (see citation below),similar to osmoprotection proteins (proX) involved in glycine betaine/L-proline/choline transport, e.g. AAK79442|CAC1474 proline/glycine betaine ABC transport system periplasmic component from Clostridium acetobutylicum (303 aa), FASTA scores: opt: 308, E(): 1.2e-11, (27.4% identity in 314 aa overlap); Q9X4J2|PROXL|SCE19A.33 PROXL protein from Streptomyces coelicolor (322 aa), FASTA scores: opt: 302, E(): 3e-11,(27.2% identity in 327 aa overlap); O29280|AF0982 osmoprotection protein (PROX) from Archaeoglobus fulgidus (292 aa), FASTA scores: opt: 235, E(): 3.4e-07, (23.15% identity in 285 aa overlap); etc. Also similar to MTV006_16 hypothetical protein from Mycobacterium tuberculosis, and MLU15180_43 hypothetical protein from Mycobacterium leprae. Equivalent to AAK48230 from Mycobacterium tuberculosis strain CDC1551 (343 aa) but shorter 28 aa. Contains probable N-terminal signal sequence.; Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) binding lipoprotein ProX 4205373 proX 886270 proX Mycobacterium tuberculosis H37Rv Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) binding lipoprotein ProX NP_218276.1 4204426 R 83332 CDS NP_218277.1 15610896 886093 4205538..4205840 1 NC_000962.3 Rv3760, (MTV025.108), len: 100 aa. Possible conserved membrane protein, equivalent to Q50094|ML2366|MLCB12.11c putative membrane protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 423, E(): 1.2e-20, (67.7% identity in 99 aa overlap). Also similar with Q9JST1|NMA2149 putative inner membrane hypothetical protein from Neisseria meningitidis (serogroup A) (104 aa),FASTA scores: opt: 113, E(): 0.95, (33.85% identity in 62 aa overlap); and showing similarity with Q9ZAX7 ABC transporter membrane protein subunit from Streptococcus mutans (498 aa), FASTA scores: opt: 108, E(): 6.7, (42.35% identity in 85 aa overlap) (similarity at C-terminus); and P33108|SECY_MICLU preprotein translocase SECY subunit from Micrococcus luteus (Micrococcus lysodeikticus) (436 aa),FASTA scores: opt: 106, E(): 8.2, (29.05% identity in 86 aa overlap). Equivalent to AAK48231 from Mycobacterium tuberculosis strain CDC1551 (117 aa) but shorter 17 aa.; Possible conserved membrane protein 4205840 886093 Rv3760 Mycobacterium tuberculosis H37Rv Possible conserved membrane protein NP_218277.1 4205538 D 83332 CDS NP_218278.1 15610897 886098 complement(4205862..4206917) 1 NC_000962.3 Rv3761c, (MTV025.109c), len: 351 aa. Possible fadE36, acyl-CoA dehydrogenase, similar to many conserved hypothetical proteins and showing some similarity with few acyl-CoA dehydrogenases, e.g. Q9APX7|FADE36 FADE36 protein from Pseudomonas aeruginosa (360 aa), FASTA scores: opt: 147, E(): 0.046, (26.15% identity in 214 aa overlap); part of AAB52261.2|U97002 protein similar to acyl-CoA dehydrogenases and epoxide hydrolases from Caenorhabditis elegans (985 aa), FASTA score: (31.2% identity in 324 aa overlap). C-terminal part is highly similar to Q50095|U1740AK|MLU15183_45 hypothetical protein from Mycobacterium leprae cosmid B174 (122 aa), FASTA scores: opt: 341, E(): 7.3e-15, (57.6% identity in 99 aa overlap). Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2.; Possible acyl-CoA dehydrogenase FadE36 4206917 fadE36 886098 fadE36 Mycobacterium tuberculosis H37Rv Possible acyl-CoA dehydrogenase FadE36 NP_218278.1 4205862 R 83332 CDS NP_218279.1 15610898 886096 complement(4206996..4208876) 1 NC_000962.3 Rv3762c, (MTV025.110c), len: 626 aa. Possible hydrolase, highly similar to hypothetical proteins and beta-lactamases e.g. Q9RL04|SC5G9.23 hypothetical 70.3 KDA protein from Streptomyces coelicolor (648 aa), FASTA scores: opt: 2088, E(): 3.7e-124, (52.9% identity in 624 aa overlap); P32717|YJCS_ECOLI|B4083 hypothetical 73.2 KDA protein from Escherichia coli strain K12 (661 aa), FASTA scores: opt: 1911, E(): 5.7e-113, (46.9% identity in 631 aa overlap); Q9A824|CC1540 metallo-beta-lactamase family protein from Caulobacter crescentus (647 aa), FASTA scores: opt: 1891, E(): 1e-111, (48.55% identity in 628 aa overlap); Q08347|YOL164W chromosome xv reading frame ORF from Saccharomyces cerevisiae (Baker's yeast) (646 aa) FASTA scores: opt: 1829, E(): 8.4e-108, (45.7% identity in 615 aa overlap); Q9I5I9|PA0740 probable beta-lactamase from Pseudomonas aeruginosa (658 aa), FASTA scores: opt: 1699,E(): 1.4e-99, (43.15% identity in 630 aa overlap); Q52556|SDSA alkyl sulfatase (protein involved in the degradation of sulfate esters of long-chain primaryal cohols e.g. SDS sodium dodecyl sulfate) from Pseudomonas sp (528 aa), FASTA scores: opt: 841, E(): 1.7e-45, (33.7% identity in 534 aa overlap); etc. N-terminual end also highly similar to Q48790|SEPA SEPA protein (protein implicated in cell separation) from Listeria monocytogenes (391 aa), FASTA scores: opt: 1256, E(): 8.3e-72, (49.6% identity in 363 aa overlap). Also slight similarity to P96253|Rv0407|MTCY22G10.03 hypothetical 37.0 KDA protein from Mycobacterium tuberculosis (336 aa).; Possible hydrolase 4208876 886096 Rv3762c Mycobacterium tuberculosis H37Rv Possible hydrolase NP_218279.1 4206996 R 83332 CDS NP_218280.1 15610899 886097 4209047..4209526 1 NC_000962.3 Rv3763, (MTV025.111), len: 159 aa. LpqH, conserved 19 KDa lipoprotein antigen precursor (see citations below),equivalent to P31502|19KD_MYCIT|MI22 19 KDA lipoprotein antigen precursor (MI22 antigen) from Mycobacterium intracellulare (162 aa), FASTA scores: opt: 773, E(): 6.2e-35, 75.95(% identity in 162 aa overlap); P46733|19KD_MYCAV 19 KDA lipoprotein antigen precursor from Mycobacterium avium (161 aa), FASTA scores: opt: 743, E(): 2.5e-33, (72.5% identity in 160 aa overlap); and Q9X7A5|LPQH|ML1966 possible lipoprotein from Mycobacterium leprae FASTA scores: opt: 371, E(): 2.2e-13, (42.6% identity in 162 aa overlap). Possibly attached to the membrane by a lipid anchor. Similar to other mycobacterium 19 KDA antigen. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; 19 kDa lipoprotein antigen precursor LpqH 4209526 lpqH 886097 lpqH Mycobacterium tuberculosis H37Rv 19 kDa lipoprotein antigen precursor LpqH NP_218280.1 4209047 D 83332 CDS NP_218281.1 15610900 886094 complement(4209582..4211009) 1 NC_000962.3 Rv3764c, (MTV025.112c), len: 475 aa. Possible tcrY,histidine protein kinase, part of a two-component regulatory system, similar to others e.g. Q9ADN6|2SC10A7.25 putative two component system histidine kinase from Streptomyces coelicolor (524 aa), FASTA scores: opt: 1332,E(): 5.4e-70, (49.9% identity in 477 aa overlap); Q9L3C1|KB|CAC42479 putative histidine kinase from Amycolatopsis mediterranei (469 aa), FASTA scores: opt: 515, E(): 1.4e-22, (36.1% identity in 313 aa overlap); P72560 histidine protein kinase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (438 aa), FASTA scores: opt: 480, E(): 1.4e-20, (40.1% identity in 232 aa overlap); P30847|P76401|BAES_ECOLI|B2078 sensor protein from Escherichia coli strain K12 (467 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. P96368|Rv1032c|MTCY10G2.17 (509 aa), FASTA scores: opt: 1007, E(): 4e-51, (43.5% identity in 416 aa overlap); and P71815|Rv0758|MTCY369.03 (485 aa), FASTA scores: opt: 738,E(): 1.6e-35, (28.6% identity in 438 aa overlap). Equivalent to AAK48235 from Mycobacterium tuberculosis strain CDC1551 (506 aa) but shorter 31 aa.; Possible two component sensor kinase TcrY 4211009 tcrY 886094 tcrY Mycobacterium tuberculosis H37Rv Possible two component sensor kinase TcrY NP_218281.1 4209582 R 83332 CDS NP_218282.1 15610901 886100 complement(4211080..4211784) 1 NC_000962.3 Rv3765c, (MTV025.113c), len: 234 aa. Probable tcrX,response regulator of a two-component regulatory system,highly similar to others e.g. Q9ADN7|2SC10A7.24 putative two component system response regulator from Streptomyces coelicolor (271 aa), FASTA scores: opt: 1111, E(): 4.8e-63,(72.3% identity in 231 aa overlap); Q9F161 response regulator from Corynebacterium glutamicum (Brevibacterium flavum) (232 aa), FASTA scores: opt: 692, E(): 1.2e-36,(46.0% identity in 226 aa overlap); Q9KZU5|SCD84.23c putative two-component systen response regulator from Streptomyces coelicolor (248 aa), FASTA scores: opt: 674,E(): 1.7e-35, (44.05% identity in 236 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q50806|Rv1033c|MTCY10G2.16 response regulator homolog (257 aa), FASTA scores: opt: 947, E(): 1e-52, (59.5% identity in 232 aa overlap); P71814|Rv0757|MTCY369.02 PHOP-like protein (247 aa) FASTA scores: opt: 829, E(): 2.8e-45, (54.65% identity in 225 aa overlap); O53894|Rv0981|MTV044.09 (230 aa), FASTA scores: opt: 662, E(): 9e-35, (44.65% identity in 224 aa overlap); and also similar to MTCY31_34; MTCY19H5_20; MTY13628_5; MTCY20G9_17; and to MLCB57_27 from Mycobacterium leprae; and MBY13627_3 from Mycobacterium bovis BCG. Equivalent to AAK48236 from Mycobacterium tuberculosis strain CDC1551 (286 aa) but shorter 52 aa. The N-terminal region is similar to that of other regulatory components of sensory transduction systems. Similar to bacterial regulatory proteins involved in signal transduction.; Probable two component transcriptional regulatory protein TcrX 4211784 tcrX 886100 tcrX Mycobacterium tuberculosis H37Rv Probable two component transcriptional regulatory protein TcrX NP_218282.1 4211080 R 83332 CDS NP_218283.1 15610902 886099 4212293..4212982 1 NC_000962.3 Rv3766, (MTV025.114), len: 229 aa. Hypothetical unknown protein. Segment 183 to 229 highly similar to C-terminal part of O06288|Rv3594|MTCY07H7B.28c conserved hypothetical protein from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 128, E(): 0.92, (46.8% identity in 47 aa overlap).; Hypothetical protein 4212982 886099 Rv3766 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218283.1 4212293 D 83332 CDS NP_218284.1 15610903 886101 complement(4212996..4213940) 1 NC_000962.3 Rv3767c, (MTV025.115c, MTCY13D12.01), len: 314 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to other Mycobacterium tuberculosis hypothetical proteins e.g. P96823|Rv0146|MTCI5.20 34.0 KDA protein (310 aa), FASTA scores: opt: 909, E(): 5.3e-50, (48.1% identity in 316 aa overlap); O53686|Rv0281|MTV035.09 (302 aa), FASTA scores: opt: 802, E(): 2.8e-43, (45.2% identity in 314 aa overlap); Q50726|YX99_MYCTU|Rv3399|MT3507|MTCY78.29c (348 aa), FASTA scores: opt: 796, E(): 7.6e-43, (45.35% identity in 302 aa overlap); MTCY78_30; MTCY31_23; MTCY210_45; MTCY4C12_14; MTY13D12_21, MTCI5_19; MTCY180_22; etc. Contains probable N-terminal signal sequence; Possible S-adenosylmethionine-dependent methyltransferase 4213940 886101 Rv3767c Mycobacterium tuberculosis H37Rv Possible S-adenosylmethionine-dependent methyltransferase NP_218284.1 4212996 R 83332 CDS NP_218285.1 15610904 886102 4214070..4214429 1 NC_000962.3 Rv3768, (MTCY13D12.02), len: 119 aa. Unknown protein.; hypothetical protein 4214429 886102 Rv3768 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218285.1 4214070 D 83332 CDS NP_218286.1 15610905 885239 4214615..4214887 1 NC_000962.3 Rv3769, (MTCY13D12.03), len: 90 aa. Hypothetical unknown protein, possible coiled-coil protein.; Hypothetical protein 4214887 885239 Rv3769 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218286.1 4214615 D 83332 CDS NP_218287.1 15610906 886104 complement(4215200..4215775) 1 NC_000962.3 Rv3770c, (MTCY13D12.04c), len: 191 aa. Hypothetical unknown leu-rich protein.; Hypothetical leucine rich protein 4215775 886104 Rv3770c Mycobacterium tuberculosis H37Rv Hypothetical leucine rich protein NP_218287.1 4215200 R 83332 CDS YP_178012.1 57117153 3205079 complement(4215881..4216063) 1 NC_000962.3 Rv3770A, len: 60 aa. Probable remnant of a transposase, similar to many e.g. Rv2812|MTCY16B7.31c|Z81331_17 IS1604 putative transposase from Mycobacterium tuberculosis (469 aa), FASTA scores: opt: 204, E(): 1e-07, (80.5% identity in 41 aa overlap). Continuation of Rv3770B.; Probable remnant of a transposase 4216063 3205079 Rv3770A Mycobacterium tuberculosis H37Rv Probable remnant of a transposase YP_178012.1 4215881 R 83332 CDS YP_178013.1 57117154 3205080 complement(4216078..4216269) 1 NC_000962.3 Rv3770B, len: 63 aa. Probable remnant of a transposase, similar to many e.g. Rv2812|MTCY16B7.31c|Z81331_17 IS1604 putative transposase from Mycobacterium tuberculosis (469 aa), FASTA scores: opt: 379, E(): 1.6e-21, (93.55% identity in 62 aa overlap). Continues as Rv3770A.; Probable remnant of a transposase 4216269 3205080 Rv3770B Mycobacterium tuberculosis H37Rv Probable remnant of a transposase YP_178013.1 4216078 R 83332 CDS NP_218288.1 15610907 886103 complement(4216404..4216730) 1 NC_000962.3 Rv3771c, (MTCY13D12.05c), len: 108 aa. Hypothetical protein, highly similar, but shorter 81 aa, to P71640|Rv2811|MTCY16B7.32c hypothetical 21.1 KDA protein from Mycobacterium tuberculosis (202 aa), FASTA scores: opt: 469, E(): 2.7e-25, (73.15% identity in 108 aa overlap); hypothetical protein 4216730 886103 Rv3771c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218288.1 4216404 R 83332 CDS NP_218289.1 15610908 886105 4217134..4218195 1 NC_000962.3 Rv3772, (MTCY13D12.06), len: 353 aa. Probable hisC2,histidinol-phosphate aminotransferase, highly similar to Q9ZBY8|SCD78.11 putative histidinol-phophate aminotransferase from Streptomyces coelicolor (359 aa),FASTA scores: opt: 1165, E(): 7.1e-64, (52.55% identity in 356 aa overlap); and similar to many e.g. Q9EYX2 from Gardnerella vaginalis (317 aa) FASTA scores: opt: 814, E(): 1.7e-42, (45.15% identity in 308 aa overlap); Q9CMI7|HISH_1PM0838|HISH from Pasteurella multocida (365 aa), FASTA scores: opt: 701, E(): 1.5e-35, (35.05% identity in 351 aa overlap); O07131|HIS8_METFL|HISC|HISH from Methylobacillus flagellatum (368 aa), FASTA scores: opt: 645, E(): 4e-32, (34.5% identity in 345 aa overlap); etc. Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate.; Probable histidinol-phosphate aminotransferase HisC2 (imidazole acetol-phosphate transaminase) (imidazolylacetolphosphate aminotransferase) 4218195 hisC2 886105 hisC2 Mycobacterium tuberculosis H37Rv Probable histidinol-phosphate aminotransferase HisC2 (imidazole acetol-phosphate transaminase) (imidazolylacetolphosphate aminotransferase) NP_218289.1 4217134 D 83332 CDS NP_218290.1 15610909 886095 complement(4218241..4218825) 1 NC_000962.3 Rv3773c, (MTCY13D12.07c), len: 194 aa. Conserved protein, highly similar to C-terminal end of O53773|Rv0576|MTV039.14 possible transcriptional regulator from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 575, E(): 8.3e-30, (47.4% identity in 192 aa overlap); and some similarity with other proteins from Mycobacterium tuberculosis e.g. P71985|Rv1727|MTCY04C12.12 (189 aa) FASTA scores: opt: 176, E(): 0.00022, (31.1% identity in 180 aa overlap).; hypothetical protein 4218825 886095 Rv3773c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218290.1 4218241 R 83332 CDS NP_218291.1 15610910 886106 4218849..4219673 1 NC_000962.3 Rv3774, (MTCY13D12.08), len: 274 aa. Possible echA21, enoyl-CoA hydratase, equivalent to Q9CD94|ECHA1|ML0120 putative enoyl-CoA hydratase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1593,E(): 2.2e-92, (88.3% identity in 274 aa overlap). Also similar to others e.g. Q9I2S4|PA1821 from Pseudomonas aeruginosa (270 aa), FASTA scores: opt: 761, E(): 2e-40,(42.3% identity in 267 aa overlap); Q9FHR8 from Arabidopsis thaliana (Mouse-ear cress) (278 aa) FASTA scores: opt: 638,E(): 9.9e-33, (39.4% identity in 269 aa overlap); Q9AB78|CC0353 from Caulobacter crescentus (286 aa), FASTA scores: opt: 601, E(): 2.1e-31, (39.25% identity in 266 aa overlap); etc.; Possible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) 4219673 echA21 886106 echA21 Mycobacterium tuberculosis H37Rv Possible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) NP_218291.1 4218849 D 83332 CDS NP_218292.1 15610911 886269 4219685..4220932 1 NC_000962.3 Rv3775, (MTCY13D12.09), len: 415 aa. Probable lipE,hydrolase lipase, equivalent to Q9CD95|LIPE|ML0119 probable hydrolase from Mycobacterium leprae (411 aa), FASTA scores: opt: 2418, E(): 6.4e-144, (84.75% identity in 406 aa overlap). Also similar to other esterases e.g. Q9ABH2|CC0255 esterase a from Caulobacter crescentus (374 aa), FASTA scores: opt: 427, E(): 2.4e-19, (28.9% identity in 391 aa overlap); O87861|ESTA esterase a from Streptomyces chrysomallus (389 aa), FASTA scores: opt: 417,E(): 1e-18, (31.0% identity in 361 aa overlap); Q9RK50|SCF12.08 putative esterase from Streptomyces coelicolor (376 aa), FASTA scores: opt: 385, E(): 1e-16,(31.35% identity in 373 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. P71778|Rv1497|MTCY277.19 hypothetical 45.8 KDA protein (429 aa), FASTA scores: opt: 457, E(): 3.5e-21, (30.4% identity in 395 aa overlap).; Probable lipase LipE 4220932 lipE 886269 lipE Mycobacterium tuberculosis H37Rv Probable lipase LipE NP_218292.1 4219685 D 83332 CDS NP_218293.1 15610912 888953 4221089..4222648 1 NC_000962.3 Rv3776, (MTCY13D12.10), len: 519 aa. Conserved hypothetical protein, highly similar to Q10709|YL00_MYCTU|Rv2100|MTCY49.40 hypothetical 58.9 KDA protein from Mycobacterium tuberculosis (550 aa) FASTA scores: opt: 1646, E(): 1.2e-83, (77.85% identity in 510 aa overlap) (homology from potential start at 7744); and similar to other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O33266|Rv0336|MTCY279.03 (503 aa) FASTA scores: opt: 682, E(): 2.2e-30, (41.65% identity in 497 aa overlap).; hypothetical protein 4222648 888953 Rv3776 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218293.1 4221089 D 83332 CDS NP_218294.1 15610913 886110 4222694..4223680 1 NC_000962.3 Rv3777, (MTCY13D12.11), len: 328 aa. Probable oxidoreductase, equivalent to Q9CD96|ML0118 putative oxidoreductase from Mycobacterium leprae (336 aa) FASTA scores: opt: 1661, E(): 1.1e-87, (76.0% identity in 325 aa overlap). Also highly similar to many e.g. Q9XA55|SCGD3.24c putative quinone oxidoreductase from Streptomyces coelicolor (326 aa) FASTA scores: opt: 1118, E(): 1.3e-64,(59.6% identity in 312 aa overlap); O65423|F18E5.200|F17L22.40|AT4G21580 putative NADPH quinone oxidoreductase from Arabidopsis thaliana (Mouse-ear cress) (325 aa), FASTA scores: opt: 1110, E(): 3e-56, (52.15% identity in 326 aa overlap); Q98FI0|MLL3767 NADPH quinone oxidoreductase from Rhizobium loti (Mesorhizobium loti) (326 aa), FASTA scores: opt: 980, E(): 7.9e-49, (47.85% identity in 324 aa overlap); etc.; Probable oxidoreductase 4223680 886110 Rv3777 Mycobacterium tuberculosis H37Rv Probable oxidoreductase NP_218294.1 4222694 D 83332 CDS NP_218295.1 15610914 886116 complement(4223699..4224895) 1 NC_000962.3 Rv3778c, (MTCY13D12.12c), len: 398 aa. Possible aminotransferase, equivalent to Q9CD97|ML0117 hypothetical protein from Mycobacterium leprae (398 aa) FASTA scores: opt: 2141, E(): 1.2e-123, (83.4% identity in 398 aa overlap). Also similar to other aminotransferases and cysteine desulfurases e.g. Q9K3K6|SCG20A.34 putative aminotransferase from Streptomyces coelicolor (400 aa),FASTA scores: opt: 723, E(): 6.5e-37, (36.3% identity in 402 aa overlap); Q9KSS2|VC1184 NIFS-related protein (aminotransferase-related) from Vibrio cholerae (416 aa) FASTA scores: opt: 595, E(): 4.5e-29, (31.35% identity in 405 aa overlap); Q98NK4|MLR0102 aminotransferase from Rhizobium loti (Mesorhizobium loti) (425 aa), FASTA scores: opt: 563, E(): 4.2e-27, (29.4% identity in 408 aa overlap); Q9RY03|DR0151 NIFS-related protein from Deinococcus radiodurans (401 aa), FASTA scores: opt: 484, E(): 2.7e-22,(32.35% identity in 399 aa overlap); Q9A766|CC1860 aminotransferase class V from Caulobacter crescentus (408 aa), FASTA scores: opt: 390, E(): 1.5e-16, (27.85% identity in 413 aa overlap); etc.; Possible aminotransferase 4224895 886116 Rv3778c Mycobacterium tuberculosis H37Rv Possible aminotransferase NP_218295.1 4223699 R 83332 CDS NP_218296.1 15610915 886107 4224985..4226985 1 NC_000962.3 Rv3779, (MTCY13D12.13), len: 666 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane ala-, leu-rich protein, equivalent to Q9CD98|ML0116 putative membrane protein from Mycobacterium leprae (654 aa), FASTA scores: opt: 1991, E(): 2e-112, (66.5% identity in 666 aa overlap). Shows some similarity with Q9RRU0|DR2395 putative NA+/H+ antiporter from Deinococcus radiodurans (458 aa), FASTA scores: opt: 138, E(): 0.69,(31.9% identity in 138 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein alanine and leucine rich 4226985 886107 Rv3779 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein alanine and leucine rich NP_218296.1 4224985 D 83332 CDS NP_218297.1 15610916 886115 4226989..4227525 1 NC_000962.3 Rv3780, (MTCY13D12.14), len: 178 aa. Conserved protein, equivalent to Q9CD99|ML0115 hypothetical 19.1 KDA protein from Mycobacterium leprae (174 aa), FASTA scores: opt: 903, E(): 2.3e-48, (82.95% identity in 170 aa overlap). Also highly similar to Q9XA56|SCGD3.23c hypothetical 19.5 KDA protein from Streptomyces coelicolor (179 aa), FASTA scores: opt: 692, E(): 1.8e-35, (65.9% identity in 170 aa overlap). Note that this putative protein is 4 aa longer at the N-terminus compared to previous annotation (in Nature 393: 537-544 (1998)).; hypothetical protein 4227525 886115 Rv3780 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218297.1 4226989 D 83332 CDS NP_218298.1 15610917 886113 4227529..4228350 1 NC_000962.3 Rv3781, (MTCY13D12.15), len: 273 aa. Probable rfbE,polysaccharide-transport ATP-binding protein ABC transporter, involved in O-antigen/lipopolysaccharides (LPS) transport (see Braibant et al., 2000), equivalent to Q9CDA0|ML0114 putative ABC transporter ATP-binding component from Mycobacterium leprae (272 aa), FASTA scores: opt: 1581, E(): 3e-83, (91.4% identity in 267 aa overlap). Also highly similar to AAK71283 LPS/O-antigen export permease from Coxiella burnetii (258 aa), FASTA scores: opt: 793, E(): 2.5e-38, (45.45% identity in 253 aa overlap); Q9PAF0|XF2568 ABC transporter ATP-binding protein from Xylella fastidiosa (246 aa), FASTA scores: opt: 758,E(): 2.4e-36, (47.75% identity in 243 aa overlap); Q56903|RFBE_YEREN O-antigen export system ATP-binding protein from Yersinia enterocolitica (239 aa) (see Zhang et al., 1993), FASTA scores: opt: 697, E(): 7e-33, (48.65% identity in 224 aa overlap); Q50863|RFBB_MYXXA O-antigen export system ATP-binding from Myxococcus xanthus (437 aa),FASTA scores: opt: 605, E(): 2e-27, (42.05% identity in 207 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters).; Probable O-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter RfbE 4228350 rfbE 886113 rfbE Mycobacterium tuberculosis H37Rv Probable O-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter RfbE NP_218298.1 4227529 D 83332 CDS YP_178014.1 57117155 886114 4228347..4229261 1 NC_000962.3 Rv3782, (MTCY13D12.16), len: 304 aa. GlfT1,UDP-galactofuranosyl transferase (See Mikusova et al.,2006; Belanova et al., 2008), equivalent to Q9CDA1|RFBE|ML0113 putative glycosyl transferase from Mycobacterium leprae (283 aa), FASTA scores: opt: 1583,E(): 9.3e-96, (81.6% identity in 277 aa overlap). Also some similarity with AAK68916|WCFN putative glycosyltransferase from Bacteroides fragilis (291 aa) FASTA scores: opt: 241,E(): 2.1e-08, (30.75% identity in 195 aa overlap); O58161|PH0424 hypothetical 40.5 KDA protein from Pyrococcus horikoshii (348 aa), FASTA scores: opt: 194, E(): 2.8e-05,(23.85% identity in 302 aa overlap); O26448|MTH348 rhamnosyl transferase from Methanothermobacter thermautotrophicus (313 aa), FASTA scores: opt: 177, E(): 0.00033, (28.2% identity in 333 aa overlap); O07868|CPS19BQ putative rhamnosyl transferase FASTA from Streptococcus pneumoniae (300 aa), FASTA scores: opt: 156, E(): 0.0074,(25.45% identity in 232 aa overlap); and other putative transferases. Note that C-terminal end shows some similarity with part of Q05161|RFB O-antigen biosynthesis protein B from Escherichia coli strain 0101. Note that previously known as rfbE.; UDP-galactofuranosyl transferase GlfT1 4229261 glfT1 886114 glfT1 Mycobacterium tuberculosis H37Rv UDP-galactofuranosyl transferase GlfT1 YP_178014.1 4228347 D 83332 CDS NP_218300.1 15610919 886111 4229258..4230100 1 NC_000962.3 Rv3783, (MTCY13D12.17), len: 280 aa. Probable rfbD,polysaccharide-transport integral membrane protein ABC transporter (see Braibant et al., 2000), involved in O-antigen/lipopolysaccharides (LPS) transport, equivalent to Q9CDA2|ML0112 putative ABC transporter component from Mycobacterium leprae (276 aa), FASTA scores: opt: 1646,E(): 4e-102, (84.3% identity in 280 aa overlap). Also highly similar to Q9PAF1|XF2567 ABC transporter permease protein from Xylella fastidiosa (267 aa), FASTA scores: opt: 723, E(): 7.6e-41, (41.3% identity in 259 aa overlap); and similar to others e.g. Q56902|RFBD_YEREN O-antigen export system permease protein from Yersinia enterocolitica (259 aa) (see Zhang et al., 1993), FASTA scores: opt: 566,E(): 2e-30, (28.05% identity in 264 aa overlap); Q06955|RFBH RFBH protein (involved in the export of lipopolysaccharide) (alias Q9KVA3|VC0246) lipopolysaccharide/O-antigen transport protein from Vibrio cholerae (257 aa), FASTA scores: opt: 358, E(): 1.3e-16,(24.4% identity in 258 aa overlap); Q9HTB8|WZM|PA5451 membrane subunit of a-band LPS efflux transporter from Pseudomonas aeruginosa (265 aa), FASTA scores: opt: 263,E(): 2.7e-10, (25.45% identity in 263 aa overlap); etc. Belongs to the ABC-2 subfamily of integral membrane proteins.; Probable O-antigen/lipopolysaccharide transport integral membrane protein ABC transporter RfbD 4230100 rfbD 886111 rfbD Mycobacterium tuberculosis H37Rv Probable O-antigen/lipopolysaccharide transport integral membrane protein ABC transporter RfbD NP_218300.1 4229258 D 83332 CDS YP_178015.1 57117156 886117 4230256..4231236 1 NC_000962.3 Rv3784, (MTCY13D12.18), len: 326 aa. Possible dTDP-glucose 4,6-dehydratase, but experimental study shown that the purified protein didn't have dTDP-glucose dehydratase (rmlB) activity (see citation below). Similar to others e.g. Q9YCT1|APE1180 long hypothetical dTDP-glucose 4,6-dehydratase from Aeropyrum pernix (330 aa) FASTA scores: opt: 598, E(): 3.7e-30, (34.9% identity in 315 aa overlap); O27817|MTH1789 dTDP-glucose 4,6-dehydratase from Methanothermobacter thermautotrophicus (336 aa) FASTA scores: opt: 587, E(): 1.8e-29, (34.9% identity in 315 aa overlap); Q9X5W0|GRSE TDP-glucose-4,6-dehydratase homolog from Streptomyces griseus (324 aa), FASTA scores: opt: 583, E(): 3.2e-29,(35.7% identity in 325 aa overlap); Q9K7J7|SPSJ|BH3364 spore coat polysaccharide synthesis (dTDP glucose 4,6-dehydratase) from Bacillus halodurans (321 aa), FASTA scores: opt: 562, E(): 6.5e-28, (33.0% identity in 318 aa overlap); Q9UZH2|RFBB|PAB0785 dTDP-glucose 4,6-dehydratase from Pyrococcus abyssi (333 aa), FASTA scores: opt: 552,E(): 2.8e-27, (33.95% identity in 318 aa overlap); P27830|RFFG_ECOLI|B3788 dTDP-glucose 4,6-dehydratase from Escherichia coli strain K12 (355 aa), FASTA scores: opt: 401, E(): 7.5e-28, (31.3% identity in 348 aa overlap); etc. But also similar to several UDP-glucose 4-epimerases and other proteins e.g. O59375|PH1742 long hypothetical UDP-glucose 4-epimerase from Pyrococcus horikoshii (306 aa) FASTA scores: opt: 600, E(): 2.6e-30, (34.5% identity in 313 aa overlap); Q9ZGC7|LANH14 NDP-hexose 4,6-dehydratase HOMOLOGfrom Streptomyces cyanogenus (326 aa), FASTA scores: opt: 593, E(): 7.6e-30, (36.45% identity in 321 aa overlap); Q57664|GALE_METJA|MJ0211 putative UDP-glucose 4-epimerase from Methanococcus jannaschii (305 aa) FASTA scores: opt: 575, E(): 9.6e-29, (32.6% identity in 313 aa overlap); etc. Seems to belong to the sugar epimerase family, dTDP-glucose dehydratase subfamily. Note that previously known as epiB.; Possible dTDP-glucose 4,6-dehydratase 4231236 886117 Rv3784 Mycobacterium tuberculosis H37Rv Possible dTDP-glucose 4,6-dehydratase YP_178015.1 4230256 D 83332 CDS NP_218302.1 15610921 886119 4231320..4232393 1 NC_000962.3 Rv3785, (MTCY13D12.19), len: 357 aa. Hypothetical unknown protein. Note that this putative protein is equivalent to AAK48258|MT3893 NAD-dependent epimerase/dehydratase family protein from Mycobacterium tuberculosis strain CDC1551 (712 aa), but shorter 355 aa.; Hypothetical protein 4232393 886119 Rv3785 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218302.1 4231320 D 83332 CDS NP_218303.1 15610922 886108 complement(4232374..4233597) 1 NC_000962.3 Rv3786c, (MTCY13D12.20), len: 407 aa. Unknown protein. Segment between aa 265-300 (approximately) is highly similar to part of O03937|RORF1608 minor capsid protein from Bacteriophage phig1e (1608 aa), FASTA scores: opt: 242, E(): 8.4e-07, (26.85% identity in 272 aa overlap); Q9ETT9|ORF36 putative peptidase from Corynebacterium equii (Rhodococcus equi) plasmid pREAT701 (p33701) and Plasmid virulence (546 aa), FASTA scores: opt: 231, E(): 1.6e-06, (34.15% identity in 167 aa overlap); O69910|SC2E1.40c hypothetical 22.8 KDA protein. from Streptomyces coelicolor (226 aa) FASTA scores: opt: 218,E(): 4.6e-06, (34.15% identity in 164 aa overlap); and others.; hypothetical protein 4233597 886108 Rv3786c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218303.1 4232374 R 83332 CDS NP_218304.1 15610923 886118 complement(4233610..4234536) 1 NC_000962.3 Rv3787c, (MTCY13D12.21), len: 308 aa. Conserved hypothetical protein, highly similar to several mycobacterial hypothetical proteins e.g. P95074|Rv0726c|MTCY210.45c from Mycobacterium tuberculosis (367 aa), FASTA scores: opt: 1038, E(): 1.6e-58, (55.85% identity in 283 aa overlap); O53795|MBE50c|Rv0731c|MTV041.05c from Mycobacterium tuberculosis (318 aa), FASTA scores: opt: 1030, E(): 4.5e-58, (56.15% identity in 292 aa overlap); Q9CCZ4|ML2640 from Mycobacterium leprae (310 aa) FASTA scores: opt: 709,E(): 9.9e-38, (43.75% identity in 279 aa overlap); etc.; hypothetical protein 4234536 886118 Rv3787c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218304.1 4233610 R 83332 CDS NP_218305.1 15610924 886120 4234780..4235265 1 NC_000962.3 Rv3788, (MTCY13D12.22), len: 161 aa. Hypothetical unknown protein.; Hypothetical protein 4235265 886120 Rv3788 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218305.1 4234780 D 83332 CDS NP_218306.1 15610925 886109 4235374..4235739 1 NC_000962.3 Rv3789, (MTCY13D12.23), len: 121 aa. GtrA family protein; possible integral membrane protein, equivalent to Q9CDA3|ML0110 hypothetical 13.9 KDA protein from Mycobacterium leprae (123 aa) FASTA scores: opt: 587, E(): 7.3e-34, (72.95% identity in 122 aa overlap). Also equivalent to AAK48262 from Mycobacterium tuberculosis strain CDC1551 (142 aa) but shorter 21 aa.; GTRA family protein 4235739 886109 Rv3789 Mycobacterium tuberculosis H37Rv GTRA family protein NP_218306.1 4235374 D 83332 CDS NP_218307.1 15610926 886125 4235779..4237164 1 NC_000962.3 Rv3790, (MTCY13D12.24), len: 461 aa. DprE1,decaprenylphosphoryl-beta-D-ribose 2'-oxidase, equivalent to Q9CDA4|ML0109 putative FAD-linked oxidoreductase from Mycobacterium leprae (460 aa), FASTA scores: opt: 2722,E(): 1.4e-161, (86.55% identity in 461 aa overlap). Also highly similar to others e.g. Q9KZA4|SC5G8.10c putative oxidoreductase from Streptomyces coelicolor (457 aa), FASTA scores: opt: 1336, E(): 1.7e-75, (47.1% identity in 452 aa overlap); Q98KY4|MLL1265 probable oxidoreductase from Rhizobium loti (Mesorhizobium loti) (449 aa), FASTA scores: opt: 636, E(): 4.9e-32, (36.0% identity in 439 aa overlap); Q9HDX8|SPAPB1A10.12c putative D-arabinono-1,4-lactone oxidase from Schizosaccharomyces pombe (Fission yeast) (461 aa), FASTA scores: opt: 297, E(): 5.6e-11, (23.55% identity in 467 aa overlap); etc. C-terminal end has a high similarity to Q9AQD0 putative oxidoreductase (fragment) from Mycobacterium smegmatis (149 aa) FASTA scores: opt: 901, E(): 6.5e-49, (86.6% identity in 149 aa overlap). Identified as the target of antimicrobial agent 1,3-benzothiazin-4-ones (BTZs) (See Makarov et al., 2009).; Decaprenylphosphoryl-beta-D-ribose 2'-oxidase 4237164 dprE1 886125 dprE1 Mycobacterium tuberculosis H37Rv Decaprenylphosphoryl-beta-D-ribose 2'-oxidase NP_218307.1 4235779 D 83332 CDS NP_218308.1 15610927 886124 4237165..4237929 1 NC_000962.3 Rv3791, (MTCY13D12.25), len: 254 aa. DprE2,decaprenylphosphoryl-D-2-keto erythro pentose reductase,equivalent to Q9CDA5|ML0108 putative oxidoreductase from Mycobacterium leprae (254 aa), FASTA scores: opt: 1458,E(): 1.6e-83, (89.0% identity in 254 aa overlap); and O05764 putative protein belonging to the short-chain alcohol dehydrogenase from Mycobacterium smegmatis (254 aa), FASTA scores: opt: 1412, E(): 1.2e-80, (85.05% identity in 254 aa overlap). Also highly similar to Q9KZA5|SC5G8.09c putative short-chain dehydrogenase from Streptomyces coelicolor (256 aa), FASTA scores: opt: 733,E(): 1.8e-38, (45.3% identity in 254 aa overlap); and P43168|YMP3_STRCO hypothetical oxidoreductase from Streptomyces coelicolor (251 aa), FASTA scores: opt: 623,E(): 1.2e-31, (42.15% identity in 254 aa overlap); and similar to various oxidoreductases (principally acetoacetyl-CoA reductases) e.g. P14697|PHBB_ALCEU acetoacetyl-CoA reductase (246 aa) from Alcaligenes eutrophus (Ralstonia eutropha) (246 aa) FASTA scores: opt: 264, E(): 2.3e-09, (29.9% identity in 204 aa overlap); P45375|PHBB_CHRVI acetoacetyl-CoA reductase from Chromatium vinosum (246 aa), FASTA scores: opt: 261, E(): 3.5e-09,(27.45% identity in 226 aa overlap); Q9RT30|DR1938 oxidoreductase (short-chain dehydrogenase/reductase family) from Deinococcus radiodurans (283 aa), FASTA scores: opt: 251, E(): 1.7e-08, (27.55% identity in 236 aa overlap); etc. Also similar to Q10681|YK73_MYCTU|Rv2073c|MT2133|MTCY49.12 putative short-chain type dehydrogenase/reductase from Mycobacterium tuberculosis (249 aa), FASTA scores: opt: 589, E(): 1.5e-29, (41.25% identity in 252 aa overlap). Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family.; Decaprenylphosphoryl-D-2-keto erythro pentose reductase 4237929 dprE2 886124 dprE2 Mycobacterium tuberculosis H37Rv Decaprenylphosphoryl-D-2-keto erythro pentose reductase NP_218308.1 4237165 D 83332 CDS NP_218309.1 15610928 886127 4237932..4239863 1 NC_000962.3 Rv3792, (MTCY13D12.26), len: 643 aa. aftA,arabinofuranosyltransferase (See Alderwick et al., 2006). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent, but longer 21 aa, to Q9CDA6|ML0107 putative membrane protein from Mycobacterium leprae (632 aa), FASTA scores: opt: 1981, E(): 2.1e-110, (77.5% identity in 631 aa overlap). C-terminal end highly similar to C-terminus of O05765 putative product ORF 3 from Mycobacterium smegmatis (603 aa), FASTA scores: opt: 1261, E(): 1.4e-67, (70.7% identity in 266 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Arabinofuranosyltransferase AftA 4239863 aftA 886127 aftA Mycobacterium tuberculosis H37Rv Arabinofuranosyltransferase AftA NP_218309.1 4237932 D 83332 CDS NP_218310.1 15610929 886112 4239863..4243147 1 NC_000962.3 Rv3793, (MTCY13D12.27), len: 1094 aa. EmbC, integral membrane protein, indolylacetylinositol arabinosyltransferase (see citations below), equivalent to Q9CDA7|EMBC|ML0106 putative arabinosyl transferase from Mycobacterium leprae (1070 aa) FASTA scores: opt: 6078,E(): 0, (82.95% identity in 1072 aa overlap); Q50393|EMBC putative arabinosyl transferase from Mycobacterium smegmatis (1074 aa), FASTA scores: opt: 5523, E(): 0,(75.35% identity in 1072 aa overlap). Also similar to Q9CDA9|EMBB| ML0104 putative arabinosyl transferase from Mycobacterium leprae (1083 aa), FASTA scores: opt: 2789,E(): 1.9e-156, (44.0% identity in 1095 aa overlap); O30406|EMBB putative arabinosyl transferase from Mycobacterium smegmatis (1082 aa), FASTA scores: opt: 2746,E(): 6.4e-154, (44.6% identity in 1096 aa overlap); etc. Also similar to to P72030|EMBB|Rv3795|MTCY13D12.29 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1098 aa), FASTA scores: opt: 2276, E(): 3.1e-126, (44.45% identity in 1118 aa overlap); and P72060|EMBA|Rv3794|MTCY13D12.28 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 1974, E(): 1.9e-108, (41.0% identity in 1110 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature; and PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Integral membrane indolylacetylinositol arabinosyltransferase EmbC (arabinosylindolylacetylinositol synthase) 4243147 embC 886112 embC Mycobacterium tuberculosis H37Rv Integral membrane indolylacetylinositol arabinosyltransferase EmbC (arabinosylindolylacetylinositol synthase) NP_218310.1 4239863 D 83332 CDS NP_218311.1 15610930 886123 4243233..4246517 1 NC_000962.3 Rv3794, (MTCY13D12.28), len: 1094 aa. EmbA, integral membrane protein, indolylacetylinositol arabinosyltransferase (see citations below), equivalent to P71485|EMBA arabinosyl transferase from Mycobacterium avium (1108 aa), FASTA scores: opt: 5024, E(): 0, (81.9% identity in 1109 aa overlap); Q9CDA8|EMBA|ML0105 putative arabinosyl transferase from Mycobacterium leprae (1111 aa), FASTA scores: opt: 4782, E(): 0, (78.6% identity in 1111 aa overlap); Q50394|EMBA putative arabinosyl transferase from Mycobacterium smegmatis (1092 aa), FASTA scores: opt: 4100,E(): 0, (67.4% identity in 1092 aa overlap). Also similar to Q9CDA7|EMBC|ML0106 putative arabinosyl transferase from Mycobacterium leprae (1070 aa), FASTA scores: opt: 1933,E(): 1.5e-100, (40.6% identity in 1108 aa overlap); Q50393|EMBC putative arabinosyl transferase from Mycobacterium smegmatis (1074 aa), FASTA scores: opt: 1870,E(): 5.1e-97, (41.4% identity in 1113 aa overlap); etc. Also similar to P72059|EMBC|Rv3793|MTCY13D12.27 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 1974, E(): 7.7e-103, (40.9% identity in 1110 aa overlap); and P72030|EMBB|Rv3795|MTCY13D12.29 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1098 aa), FASTA scores: opt: 1288, E(): 2.1e-64, (42.5% identity in 1114 aa overlap). Supposedly regulated by embR|Rv1267c. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Integral membrane indolylacetylinositol arabinosyltransferase EmbA (arabinosylindolylacetylinositol synthase) 4246517 embA 886123 embA Mycobacterium tuberculosis H37Rv Integral membrane indolylacetylinositol arabinosyltransferase EmbA (arabinosylindolylacetylinositol synthase) NP_218311.1 4243233 D 83332 CDS NP_218312.1 15610931 886126 4246514..4249810 1 NC_000962.3 Rv3795, (MTCY13D12.29), len: 1098 aa. EmbB, integral membrane protein, indolylacetylinositol arabinosyltransferase (see citations below), equivalent to P71486|EMBB arabinosyl transferase from Mycobacterium avium (1065 aa), FASTA scores: opt: 4998, E(): 0, (83.25% identity in 1076 aa overlap); Q9CDA9|EMBB|ML0104 putative arabinosyl transferase from Mycobacterium leprae (1083 aa),FASTA scores: opt: 4706, E(): 0, (78.0% identity in 1101 aa overlap); O30406|EMBB (alias Q50395) putative arabinosyl transferase from Mycobacterium smegmatis (1082 aa), FASTA scores: opt: 4163, E(): 0, (68.4% identity in 1091 aa overlap); etc. Also similar to Q50393|EMBC putative arabinosyl transferase from Mycobacterium smegmatis (1074 aa), FASTA scores: opt: 2482, E(): 5e-135, (44.7% identity in 1101 aa overlap); Q9CDA7|EMBC|ML0106 putative arabinosyl transferase from Mycobacterium leprae (1070 aa), FASTA scores: opt: 2259, E(): 3.4e-122, (43.4% identity in 1104 aa overlap); etc. Also similar to P72059|EMBC|Rv3793|MTCY13D12.27 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 2276, E(): 3.6e-123, (44.45% identity in 1118 aa overlap); and P72060|EMBA|Rv3794|MTCY13D12.28 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 1288, E(): 2.5e-66, (42.35% identity in 1114 aa overlap). Supposedly regulated by embR|Rv1267c. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Integral membrane indolylacetylinositol arabinosyltransferase EmbB (arabinosylindolylacetylinositol synthase) 4249810 embB 886126 embB Mycobacterium tuberculosis H37Rv Integral membrane indolylacetylinositol arabinosyltransferase EmbB (arabinosylindolylacetylinositol synthase) NP_218312.1 4246514 D 83332 CDS YP_178016.1 57117157 886128 4249878..4251005 1 NC_000962.3 Rv3796, (MTV026.01-MTCY13D12.30), len: 375 aa. Conserved protein. C-terminal end similar in part to Q983J3|MLR8305 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (227 aa), FASTA scores: opt: 288, E(): 4e-09, (38.95% identity in 154 aa overlap). Similar to P54548|YQJK_BACSU hypothetical protein (belongs to the ATSA/ELAC family) from Bacillus subtilis (307 aa) FASTA scores: opt: 263, E(): 1.3e-07, (26.1% identity in 295 aa overlap); and some similarity to other proteins e.g. AAK46775|MT2479 putative arylsulfatase from Mycobacterium tuberculosis strain CDC1551 (224 aa), FASTA scores: opt: 194, E(): 0.00072, (25.85% identity in 259 aa overlap). Equivalent to AAK48269 from Mycobacterium tuberculosis strain CDC1551 (338 aa) but longer 37 aa. Some similarity to the A. carrageenovora AtsA / E. coli ElaC family. Note that previously known as atsH. Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 4251005 886128 Rv3796 Mycobacterium tuberculosis H37Rv hypothetical protein YP_178016.1 4249878 D 83332 CDS NP_218314.1 15610933 886122 4251085..4252866 1 NC_000962.3 Rv3797, (MTV026.02), len: 593 aa. Probable fadE35,acyl-CoA dehydrogenase, similar to many e.g. Q9HY33|PA3593 from Pseudomonas aeruginosa (575 aa) FASTA scores: opt: 838, E(): 2.1e-46, (35.3% identity in 569 aa overlap); Q9ANZ8|AIDB from Burkholderia pseudomallei (Pseudomonas pseudomallei) (554 aa), FASTA scores: opt: 633, E(): 3.4e-33, (33.1% identity in 480 aa overlap); Q9HX44|PA3972 from Pseudomonas aeruginosa (549 aa) FASTA scores: opt: 560, E(): 1.7e-28, (29.9% identity in 569 aa overlap); P33224|AIDB_ECOLI|B4187 from Escherichia coli strain K12 (541 aa), FASTA scores: opt: 455, E(): 1e-21, (31.15% identity in 514 aa overlap); etc. Also similar to O86368|FADE8|Rv0672|MTCI376.02c acyl-CoA dehydrogenase from Mycobacterium tuberculosis (542 aa), FASTA scores: opt: 479, E(): 2.9e-23, (32.2% identity in 460 aa overlap). Could belong to the acyl-CoA dehydrogenases family.; Probable acyl-CoA dehydrogenase FadE35 4252866 fadE35 886122 fadE35 Mycobacterium tuberculosis H37Rv Probable acyl-CoA dehydrogenase FadE35 NP_218314.1 4251085 D 83332 CDS NP_218315.1 15610934 886268 4252993..4254327 1 NC_000962.3 Rv3798, (MTV026.03), len: 444 aa. Probable transposase for insertion sequence element IS1557, highly similar to Q60255 similar to transposase of ISAE1 from alcaligenes eutrophus H1-4 (fragment) from dibenzofuran-degrading bacterium DPO360 (163 aa) FASTA scores: opt: 767, E(): 3.2e-42, (67.25% identity in 168 aa overlap); and similar to P74920 transposase from Thiobacillus ferrooxidans (404 aa), FASTA scores: opt: 375,E(): 1.1e-16, (27.55% identity in 439 aa overlap); Q48349 transposase from Alcaligenes eutrophus (Ralstonia eutropha) (408 aa), FASTA scores: opt: 324, E(): 2e-13, (3.9% identity in 369 aa overlap); Q9FDC1|TNP transposase from Burkholderia mallei (Pseudomonas mallei) (386 aa) FASTA scores: opt: 282, E(): 9.8e-11, (25.85% identity in 391 aa overlap); etc. C-terminal end identical to O53804|Rv0741|MTV041.15 transposase from Mycobacterium tuberculosis (104 aa), FASTA scores: opt: 582, E(): 1.8e-30, (85.6% identity in 104 aa overlap). Belongs to the transposase family 12.; Probable transposase 4254327 886268 Rv3798 Mycobacterium tuberculosis H37Rv Probable transposase NP_218315.1 4252993 D 83332 CDS NP_218316.2 57117158 886131 complement(4254380..4255948) 1 NC_000962.3 Rv3799c, (MTV026.04c), len: 522 aa. Probable accD4,propionyl-CoA carboxylase beta chain 4, equivalent to Q9CDB0|ACCD4|ML0102 putative acyl CoA carboxylase from Mycobacterium leprae (517 aa) FASTA scores: opt: 3154, E(): 8e-187, (91.2% identity in 511 aa overlap). Also similar to many e.g. Q9X4K7|PCCB from Streptomyces coelicolor (530 aa), FASTA scores: opt: 1714, E(): 4.4e-98, (50.0% identity in 510 aa overlap); P53003|PCCB_SACER from Saccharopolyspora erythraea (Streptomyces erythraeus) (546 aa), FASTA scores: opt: 1549, E(): 6.6e-88, (50.65% identity in 519 aa overlap); Q9WZH5|TM0716 from Thermotoga maritima (515 aa) FASTA scores: opt: 1529, E(): 1.1e-86,(46.7% identity in 512 aa overlap); etc. Also similar to P53002|PCCB_MYCLE|ACCD5|PCCB|ML0731|B1308_C1_125 probable propionyl-CoA carboxylase beta chain 5 from Mycobacterium leprae (549 aa), FASTA scores: opt: 1493, E(): 1.9e-84,(49.8% identity in 514 aa overlap); and P96885|PCC5_MYCTU|ACCD5|PCCB|Rv3280|MT3379.1|MTCY71.20 probable propionyl-CoA carboxylase beta chain 5 from Mycobacterium tuberculosis (548 aa), FASTA scores: opt: 1471, E(): 4.2e-83, (49.15% identity in 515 aa overlap). Belongs to the ACCD/PCCB family. Length extended since first submission (+5 aa). AccA3 (Rv3285), AccD5 (Rv3280),AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006).; Probable propionyl-CoA carboxylase beta chain 4 AccD4 (pccase) (propanoyl-CoA:carbon dioxide ligase) 4255948 accD4 886131 accD4 Mycobacterium tuberculosis H37Rv Probable propionyl-CoA carboxylase beta chain 4 AccD4 (pccase) (propanoyl-CoA:carbon dioxide ligase) NP_218316.2 4254380 R 83332 CDS NP_218317.1 15610936 886133 complement(4255945..4261146) 1 NC_000962.3 Rv3800c, (MTV026.05c), len: 1733 aa. Probable pks13,polyketide synthase, equivalent to Q9CDB1|PKS13|ML0101 polyketide synthase from Mycobacterium leprae (1784 aa),FASTA scores: opt: 7454, E(): 0, (83.6% identity in 1748 aa overlap); and similar to Q9Z5K6|ML2357|MLCB12.02c putative polyketide synthase from Mycobacterium leprae (1871 aa),FASTA scores: opt: 1682, E(): 1.2e-85, (38.3% identity in 1096 aa overlap). Also similar in part to many e.g. Q9ADL6|SORA soraphen polyketide synthase a from Polyangium cellulosum (6315 aa) FASTA scores: opt: 1422, E(): 1e-70,(31.45% identity in 1616 aa overlap); AAK73501|AMPHI AMPHI protein (involved in amphotericin biosynthesis) from Streptomyces nodosus (9510 aa), FASTA scores: opt: 1441,E(): 1.2e-71, (30.45% identity in 1662 aa overlap); Q9RFL0|MTAB MTAB protein (involved in myxothiazol biosynthesis) from Stigmatella aurantiaca (4003 aa), FASTA scores: opt: 1429, E(): 2.8e-71, (33.8% identity in 1089 aa overlap); Q9L4X2|NYSJ from Streptomyces noursei (5435 aa),FASTA scores: opt: 1407, E(): 6.1e-70, (30.5% identity in 1764 aa overlap); CAC37876|SC1G7.01c from Streptomyces coelicolor (3489 aa) FASTA scores: opt: 1382, E(): 1e-68,(31.05% identity in 1489 aa overlap); etc. Also highly similar to Q10977|PPSA_MYCTU|Rv2931|MT3000|MTCY338.20 phenolpthiocerol synthesis polyketide synthase from Mycobacterium tuberculosis (1876 aa), FASTA scores: opt: 1728, E(): 3.4e-88, (36.95% identity in 1269 aa overlap); and P96203|PPSD|Rv2934|MTCY19H9.02. Contains PS00606 Beta-ketoacyl synthases active site.; Polyketide synthase Pks13 4261146 pks13 886133 pks13 Mycobacterium tuberculosis H37Rv Polyketide synthase Pks13 NP_218317.1 4255945 R 83332 CDS NP_218318.1 15610937 886130 complement(4261153..4263066) 1 NC_000962.3 Rv3801c, (MTV026.06c), len: 637 aa. FadD32,fatty-acid-AMP synthetase, equivalent to Q9CDB2|FADD32|ML0100 putative acyl-CoA synthetase from Mycobacterium leprae (635 aa), FASTA scores: opt: 3892,E(): 0, (93.05% identity in 632 aa overlap); and highly similar to others from Mycobacterium leprae. Also similar to others from Mycobacterium tuberculosis e.g. P95288|FADD31|Rv1925|MTCY09F9.39c (620 aa), FASTA scores: opt: 1567, E(): 1.7e-88, (47.05% identity in 612 aa overlap); MTCY338_18, MTCY349_40, MTV005_21, MTCY24G1_8,MTCY19G5_7, MTCY4D9_17; and MBU75685_1 acyl-CoA ligase from Mycobacterium bovis.; Fatty-acid-AMP ligase FadD32 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Also shown to have acyl-ACP ligase activity 4263066 fadD32 886130 fadD32 Mycobacterium tuberculosis H37Rv Fatty-acid-AMP ligase FadD32 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Also shown to have acyl-ACP ligase activity NP_218318.1 4261153 R 83332 CDS NP_218319.1 15610938 886135 complement(4263355..4264365) 1 NC_000962.3 Rv3802c, (MTV026.07c), len: 336 aa. Probable conserved membrane protein, with a N-terminal signal sequence followed by Pro-rich region. Equivalent to Q9CDB3|ML0099 hypothetical protein from Mycobacterium leprae (336 aa) FASTA scores: opt: 1759, E(): 1.1e-85,(75.5% identity in 335 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008).; Probable conserved membrane protein 4264365 886135 Rv3802c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_218319.1 4263355 R 83332 CDS YP_178017.1 57117159 886121 complement(4264563..4265462) 1 NC_000962.3 Rv3803c, (MT3910, MTV026.08c), len: 299 aa. FbpD (alternate gene names: mpt51, mpb51, fbpC1), secreted MPB51/MPT51 antigen protein (fibronectin-binding protein C) (mycolyl transferase 85C) (see citations below), identical to Q48923|MPT51|MPB51 antigen precursor from Mycobacterium bovis (299 aa), FASTA scores: opt: 2093, E(): 1.5e-112,(100.0% identity in 299 aa overlap) (see Ohara et al.,1995); and highly similar to other Mycobacterial antigen precursors e.g. Q05868|MPT5_MYCLE|MPT51|ML0098 MPT51 antigen precursor from Mycobacterium leprae (301 aa), FASTA scores: opt: 1624, E(): 9.8e-86, (77.8% identity in 302 aa overlap); O52972|A85C_MYCAV|FBPC antigen 85-C precursor (fibronectin-binding protein C) from Mycobacterium avium (352 aa), FASTA scores: opt: 753, E(): 6.6e-36, (41.5% identity in 315 aa overlap); P21160|A85B_MYCKA antigen 85-B precursor (fibronectin-binding protein B) from Mycobacterium kansasii (325 aa), FASTA scores: opt: 574,E(): 1.1e-25, (37.55% identity in 309 aa overlap); P12942|A85B_MYCBO antigen 85-B precursor from Mycobacterium bovis (323 aa), FASTA scores: opt: 572, E(): 1.4e-25,(39.85% identity in 291 aa overlap); etc. Also similar to P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 secreted antigen 85-C (mycolyl transferase 85C) (fibronectin-binding protein C) from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 751, E(): 8.4e-36, (40.65% identity in 310 aa overlap); P17944|A85A_MYCTU|FBPA|MPT44|Rv3804c|MT3911|MTV026.09c secreted antigen 85-a (mycolyl transferase 85A) (fibronectin-binding protein A) from Mycobacterium tuberculosis (338 aa), FASTA scores: opt: 592, E(): 1e-26,(39.05% identity in 302 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Note that the secreted protein MPB51 is one of the major proteins in the culture filtrate of Mycobacterium bovis BCG. Note that overexpression in an FbpC-deficient M. tuberculosis clinical isolate has no effect on the amount of cell wall-linked mycolates (See Puech et al., 2002).; Secreted MPT51/MPB51 antigen protein FbpD (MPT51/MPB51 antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) (85C) 4265462 fbpD 886121 fbpD Mycobacterium tuberculosis H37Rv Secreted MPT51/MPB51 antigen protein FbpD (MPT51/MPB51 antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) (85C) YP_178017.1 4264563 R 83332 CDS NP_218321.1 15610940 886132 complement(4265642..4266658) 1 NC_000962.3 Rv3804c, (MT3911, MTV026.09c), len: 338 aa. FbpA (alternate gene names: mpt44, 85A), precursor of the 85-a antigen (fibronectin-binding protein A) (mycolyl transferase 85A) (see citations below), identical to P17944|P17996|FBPA|MPT44 antigen 85-a precursor from Mycobacterium bovis (338 aa), FASTA scores: opt: 2341, E(): 1.2e-132, (100.0% identity in 338 aa overlap); and highly similar to other Mycobacterial antigen precursors e.g. O52956|A85A_MYCAV|FBPA antigen 85-a precursor (85A) from Mycobacterium avium (347 aa), FASTA scores: opt: 1987, E(): 1.7e-111, (82.55% identity in 338 aa overlap); Q05861|A85A_MYCLE|FBPA|ML0097 antigen 85-a precursor (85A) from Mycobacterium leprae (330 aa), FASTA scores: opt: 1936, E(): 1.9e-108, (83.0% identity in 329 aa overlap); O06052|A85A_MYCGO|FBPA antigen 85-a precursor (85A) from Mycobacterium gordonae (339 aa), FASTA scores: opt: 1932,E(): 3.3e-108, (80.45% identity in 338 aa overlap); etc. Also highly similar to P31952|A85B_MYCTU|FBPB|Rv1886c|MT1934|MTCY180.32 secreted antigen 85-B from Mycobacterium tuberculosis (325 aa),FASTA scores: opt: 1830, E(): 3.9e-102, (78.85% identity in 317 aa overlap); P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 secreted antigen 85-C from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 1597, E(): 3.4e-88, (67.25% identity in 336 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008).; Secreted antigen 85-a FbpA (mycolyl transferase 85A) (fibronectin-binding protein A) (antigen 85 complex A) 4266658 fbpA 886132 fbpA Mycobacterium tuberculosis H37Rv Secreted antigen 85-a FbpA (mycolyl transferase 85A) (fibronectin-binding protein A) (antigen 85 complex A) NP_218321.1 4265642 R 83332 CDS NP_218322.1 15610941 886138 complement(4266953..4268836) 1 NC_000962.3 Rv3805c, (MTV026.10c), len: 627 aa. Possible aftB,arabinofuranosyltransferase (See Seidel et al., 2007). Probable conserved transmembrane protein, equivalent, but shorter 19 aa, to Q9CDB4|ML0096 putative membrane protein from Mycobacterium leprae (649 aa), FASTA scores: opt: 3511, E(): 1.1e-204, (80.9% identity in 629 aa overlap). Equivalent to AAK48278 from Mycobacterium tuberculosis strain CDC1551 (641 aa) but shorter 14 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible arabinofuranosyltransferase AftB 4268836 aftB 886138 aftB Mycobacterium tuberculosis H37Rv Possible arabinofuranosyltransferase AftB NP_218322.1 4266953 R 83332 CDS NP_218323.1 15610942 886129 complement(4268925..4269833) 1 NC_000962.3 Rv3806c, (MTV026.11c), len: 302 aa. UbiA,decaprenylphosphoryl-5-phosphoribose (DPPR) synthase (See Huang et al., 2005), equivalent to Q9CDB5|ML0095 putative integral membrane protein from Mycobacterium leprae (302 aa), FASTA scores: opt: 1677, E(): 3.9e-103, (83.75% identity in 302 aa overlap). Also highly similar to others e.g. Q9KZA2|SC5G8.12 putative integral membrane protein from Streptomyces coelicolor (322 aa), FASTA scores: opt: 937, E(): 2e-54, (51.4% identity in 292 aa overlap); AAK79783|CAC1818 conserved membrane protein, possible 4-hydroxybenzoate from Clostridium acetobutylicum (290 aa),FASTA scores: opt: 467, E(): 1.5e-23, (26.9% identity in 290 aa overlap); Q98KY3|MLL1266 nodulation protein NOEC (potential integral membrane protein) from Rhizobium loti (Mesorhizobium loti) (297 aa), FASTA scores: opt: 331, E(): 1.4e-14, (27.4% identity in 299 aa overlap); etc. And highly similar to C-terminal part of Q981F8|MLR9393 nodulation protein NOEC (potential integral membrane protein) from Rhizobium loti (Mesorhizobium loti) plasmid pMLa (541 aa), FASTA scores: opt: 388, E(): 4e-18, (30.9% identity in 301 aa overlap); and P55585|Y4NM_RHISN integral membrane protein (possible permease/transporter) from Rhizobium sp. strain NGR234 plasmid sym pNGR234a (516 aa),FASTA scores: opt: 380, E(): 1.3e-17, (31.85% identity in 295 aa overlap). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature.; Decaprenylphosphoryl-5-phosphoribose (DPPR) synthase (decaprenyl-phosphate 5-phosphoribosyltransferase) 4269833 ubiA 886129 ubiA Mycobacterium tuberculosis H37Rv Decaprenylphosphoryl-5-phosphoribose (DPPR) synthase (decaprenyl-phosphate 5-phosphoribosyltransferase) NP_218323.1 4268925 R 83332 CDS NP_218324.1 15610943 886134 complement(4269840..4270337) 1 NC_000962.3 Rv3807c, (MTV026.12), len: 165 aa. Possible conserved transmembrane protein, equivalent to Q9CDB6|ML0094 putative membrane protein from Mycobacterium leprae (192 aa), FASTA scores: opt: 714, E(): 2.4e-38,(72.85% identity in 151 aa overlap). Also highly similar to Q9KZA3|SC5G8.11 putative integral membrane protein from Streptomyces coelicolor (169 aa), FASTA scores: opt: 324,E(): 1.1e-13, (41.5% identity in 159 aa overlap); and similar in part to others e.g. Q9K3L3|SCG20A.27 putative integral membrane protein from Streptomyces coelicolor (230 aa), FASTA scores: opt: 277, E(): 1.3e-10, (41.65% identity in 168 aa overlap); P72269|ORF8 hypothetical protein from Rhodococcus erythropolis (487 aa) FASTA scores: opt: 229,E(): 2.7e-07, (36.25% identity in 149 aa overlap); O86625|SC3A7.24c putative integral membrane protein from Streptomyces coelicolor (201 aa) FASTA scores: opt: 200,E(): 9.1e-06, (34.95% identity in 146 aa overlap); Q9KYD7|SCD72A.19 putative integral membrane protein from Streptomyces coelicolor (238 aa) FASTA scores: opt: 178,E(): 0.00026, (35.7% identity in 112 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Possible conserved transmembrane protein 4270337 886134 Rv3807c Mycobacterium tuberculosis H37Rv Possible conserved transmembrane protein NP_218324.1 4269840 R 83332 CDS NP_218325.1 15610944 886136 complement(4270366..4272279) 1 NC_000962.3 Rv3808c, (MTV026.13c), len: 637 aa. GlfT2,bifunctional UDP-galactofuranosyl transferase (see citations below). Equivalent to Q9CDB7|ML0093 hypothetical protein from Mycobacterium leprae (643 aa), FASTA scores: opt: 3751, E(): 0, (85.4% identity in 643 aa overlap). Contains a beta-glycosyltransferase domain A. Note that previously known as glfT. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).; Bifunctional UDP-galactofuranosyl transferase GlfT2 4272279 glfT2 886136 glfT2 Mycobacterium tuberculosis H37Rv Bifunctional UDP-galactofuranosyl transferase GlfT2 NP_218325.1 4270366 R 83332 CDS NP_218326.1 15610945 886142 complement(4272276..4273475) 1 NC_000962.3 Rv3809c, (MTV026.14), len: 399 aa. Glf (alternate gene name: ceoA), UDP-galactopyranose mutase (see citations below), identical to previously sequenced gene, and equivalent to Q9CDB8|GLF|ML0092 putative UDP-galactopyranose mutase from Mycobacterium leprae (413 aa), FASTA scores: opt: 2347, E(): 1.3e-140, (86.6% identity in 396 aa overlap). Also highly similar to others e.g. AAK61905|EPSJ UDP-galactopyranose mutase (protein involved in exopolysaccharides biosynthesis) from Streptococcus thermophilus (365 aa), FASTA scores: opt: 972, E(): 5.9e-54, (45.85% identity in 375 aa overlap); P37747|GLF_ECOLI|B2036 UDP-galactopyranose mutase from Escherichia coli strain K12 (367 aa), FASTA scores: opt: 958, E(): 4.5e-53, (43.55% identity in 379 aa overlap); O86897|CAP33FN from Streptococcus pneumoniae (369 aa) FASTA scores: opt: 954, E(): 8.1e-53, (44.8% identity in 375 aa overlap); etc. Cofactor: FAD (by similarity). N-terminal SHOWS similarity to FAD or NAD containing proteins.; UDP-galactopyranose mutase Glf (UDP-GALP mutase) (NAD+-flavin adenine dinucleotide-requiring enzyme) 4273475 glf 886142 glf Mycobacterium tuberculosis H37Rv UDP-galactopyranose mutase Glf (UDP-GALP mutase) (NAD+-flavin adenine dinucleotide-requiring enzyme) NP_218326.1 4272276 R 83332 CDS NP_218327.1 15610946 886139 4273739..4274593 1 NC_000962.3 Rv3810, (MTV026.15), len: 284 aa. PirG (alternate gene names: P36 or erp for Exported Repeated Protein), cell surface protein precursor (see citations below), equivalent to P19361|28KD_MYCLE|ML0091 28 KDA antigen precursor from Mycobacterium leprae (236 aa), FASTA scores: opt: 555, E(): 9.8e-18, (52.65% identity in 281 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Exported repetitive protein precursor PirG (cell surface protein) (EXP53) 4274593 pirG 886139 pirG Mycobacterium tuberculosis H37Rv Exported repetitive protein precursor PirG (cell surface protein) (EXP53) NP_218327.1 4273739 D 83332 CDS YP_178018.1 57117160 886137 4274798..4276417 1 NC_000962.3 Rv3811, (MTV026.16), len: 539 aa. Conserved hypothetical protein, showing some similarity to Q9KZK5|SCE34.21c putative secreted protein from Streptomyces coelicolor (416 aa), FASTA scores: opt: 603,E(): 8.1e-26, (34.4% identity in 404 aa overlap); Q9S2P9|SC5F7.14c hypothetical 31.9 KDA protein from Streptomyces coelicolor (308 aa), FASTA scores: opt: 472,E(): 9.5e-19, (37.5% identity in 208 aa overlap). Middle section (approximately aa 185-350/390) shows some similarity with Q9GK12 peptidoglycan recognition protein precursor from Camelus dromedarius (Dromedary) (Arabian camel) (193 aa) FASTA scores: opt: 274, E(): 4.6e-08,(32.2% identity in 177 aa overlap); O75594|PGLYRP|PGRP from Homo sapiens (Human) (196 aa), FASTA scores: opt: 272, E(): 6e-08, (30.9% identity in 220 aa overlap); Q9JLN4|PGRP peptidoglycan recognition protein from Rattus norvegicus (Rat) (182 aa), FASTA scores: opt: 253, E(): 6.2e-07,(32.15% identity in 171 aa overlap); etc. C-terminal end shows similarity with Q01377|CSP1_CORGL PS1 protein precursor (one of the two major secreted proteins) from Corynebacterium glutamicum (Brevibacterium flavum) (657 aa), FASTA scores: opt: 250, E(): 2.7e-06, (39.45% identity in 109 aa overlap). Contains PS00687 Aldehydedehydrogenases glutamic acid active site. Note that previously known as csp.; hypothetical protein 4276417 886137 Rv3811 Mycobacterium tuberculosis H37Rv hypothetical protein YP_178018.1 4274798 D 83332 CDS YP_178019.1 57117161 886143 4276571..4278085 1 NC_000962.3 Rv3812, (MTV026.17, MTCY409.18c), len: 504 aa. PE_PGRS62, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citations below), similar to many e.g. P96828|Rv0151c|MTCI5.25c (588 aa), FASTA scores: opt: 389, E(): 6.2e-14, (29.2% identity in 473 aa overlap); MTCY7H7B_27; MTCY493_24; MTCY441_4; MTCY39_36; MTCY1A11_4; MTCY359_33; MTCY130_10; MTCY98_9; etc. The transcription of this CDS seems to be activated in macrophages (see Ramakrishnan et al., 2000).; PE-PGRS family protein PE_PGRS62 4278085 PE_PGRS62 886143 PE_PGRS62 Mycobacterium tuberculosis H37Rv PE-PGRS family protein PE_PGRS62 YP_178019.1 4276571 D 83332 CDS NP_218330.1 15610949 886147 complement(4278394..4279215) 1 NC_000962.3 Rv3813c, (MTCY409.17), len: 273 aa. Conserved protein, equivalent to Q9CDB9|ML0089 hypothetical protein from Mycobacterium leprae (281 aa) FASTA scores: opt: 1479,E(): 9.6e-81, (80.45% identity in 271 aa overlap); and similar to Q98LI0|MLL1014 from (280 aa). Also similar to many hypothetical proteins from several organisms e.g. Q9ZBX2|SCD78.27c from Streptomyces coelicolor (280 aa),FASTA scores: opt: 597, E(): 2.2e-28, (43.25% identity in 266 aa overlap); Q9RXR7|DR0240 from Deinococcus radiodurans (284 aa), FASTA scores: opt: 543, E(): 3.5e-25, (38.65% identity in 264 aa overlap); Q99YH5|SPY1700 from Streptococcus pyogenes (274 aa) FASTA scores: opt: 373,E(): 4.3e-15, (30.75% identity in 270 aa overlap); P70947|YITU from Bacillus subtilis (270 aa) FASTA scores: opt: 353, E(): 6.5e-14, (30.0% identity in 280 aa overlap); etc.; hypothetical protein 4279215 886147 Rv3813c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218330.1 4278394 R 83332 CDS NP_218331.1 15610950 886150 complement(4279230..4280015) 1 NC_000962.3 Rv3814c, (MTCY409.16), len: 261 aa. Possible acyltransferase, highly similar to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa), FASTA scores: opt: 753, E(): 7.7e-42, (46.75% identity in 246 aa overlap). Also highly similar to many acyltransferases and hypothetical proteins e.g. Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa),FASTA scores: opt: 587, E(): 4.6e-31, (41.95% identity in 243 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 293, E(): 6.6e-12, (29.2% identity in 267 aa overlap); Q9PNZ5|AAS|CJ0938 putative 2-acylglycerophosphoethanolamine acyltransferase / acyl-acyl carrier protein synthetase from Campylobacter jejuni (1170 aa), FASTA scores: opt: 274,E(): 3.9e-10, (29.1% identity in 219 aa overlap) (similarity only with middle section); Q9EY25 putative acetyl transferase from Xanthomonas oryzae pv. oryzae (249 aa), FASTA scores: opt: 238, E(): 2.4e-08, (29.2% identity in 209 aa overlap); etc. Also highly similar to downstream ORFs O07808|Rv3815c|MTCY409.15 putative acyltransferase from Mycobacterium tuberculosis (251 aa), FASTA scores: opt: 1069, E(): 2.1e-62, (60.4% identity in 245 aa overlap); and O07807|Rv3816c|MTCY409.14 putative acyltransferase from Mycobacterium tuberculosis (259 aa),FASTA scores: opt: 776, E(): 2.5e-43, (50.9% identity in 228 aa overlap). And similar to O53516|Rv2182c|MTV021.15c hypothetical 27.0 KDA protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 239, E(): 2e-08,(30.6% identity in 232 aa overlap).; Possible acyltransferase 4280015 886150 Rv3814c Mycobacterium tuberculosis H37Rv Possible acyltransferase NP_218331.1 4279230 R 83332 CDS NP_218332.1 15610951 886149 complement(4280033..4280788) 1 NC_000962.3 Rv3815c, (MTCY409.15), len: 251 aa. Possible acyltransferase, highly similar to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa), FASTA scores: opt: 845, E(): 2.7e-47, (53.25% identity in 246 aa overlap). Also highly similar to Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa),FASTA scores: opt: 656, E(): 3.7e-35, (47.85% identity in 234 aa overlap); and similar to many putative acyltransferases and hypothetical proteins e.g. P74498|SLL1848 hypothetical 24.3 KDA protein from Synechocystis sp. strain PCC 6803 (225 aa) FASTA scores: opt: 275, E(): 1.2e-10, (34.8% identity in 181 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 266, E(): 5.2e-10,(29.7% identity in 229 aa overlap); Q9PNZ5|AAS|CJ0938 putative 2-acylglycerophosphoethanolamine acyltransferase/ acyl-acyl carrier protein synthetase from Campylobacter jejuni (1170 aa), FASTA scores: opt: 264, E(): 2.3e-09,(23.55% identity in 221 aa overlap) (similarity only with middle section); etc. Also highly similar to upstream ORF O07809|Rv3814c|MTCY409.16 putative acyltransferase from Mycobacterium tuberculosis (261 aa), FASTA scores: opt: 1069, E(): 1e-61, (60.4% identity in 245 aa overlap) ; and downstream ORF O07807|Rv3816c|MTCY409.14 putative acyltransferase from Mycobacterium tuberculosis (259 aa) FASTA scores: opt: 847, E(): 2e-47, (55.7% identity in 246 aa overlap). And similar to O53516|Rv2182c|MTV021.15c hypothetical 27.0 KDA protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 237, E(): 3.6e-08, (30.9% identity in 233 aa overlap).; Possible acyltransferase 4280788 886149 Rv3815c Mycobacterium tuberculosis H37Rv Possible acyltransferase NP_218332.1 4280033 R 83332 CDS NP_218333.1 15610952 886152 complement(4280792..4281571) 1 NC_000962.3 Rv3816c, (MTCY409.14), len: 259 aa. Possible acyltransferase, equivalent to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa) FASTA scores: opt: 1401, E(): 1.5e-80, (81.9% identity in 254 aa overlap). Also highly similar to many putative acyltransferases and hypothetical proteins e.g. Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa), FASTA scores: opt: 758, E(): 2.4e-40,(51.7% identity in 234 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 312, E(): 2e-12, (29.55% identity in 237 aa overlap); O67841|AAS|AQ_2058 2-acylglycerophosphoethanolamine acyltransferase from Aquifex aeolicus (211 aa), FASTA scores: opt: 281, E(): 1.5e-10, (32.7% identity in 162 aa overlap); etc. Also highly similar to upstream ORFs O07808|Rv3815c|MTCY409.15 putative acyltransferase from Mycobacterium tuberculosis (251 aa), FASTA scores: opt: 847, E(): 6.7e-46, (55.7% identity in 246 aa overlap); and O07809|Rv3814c|MTCY409.16 putative acyltransferase from Mycobacterium tuberculosis (261 aa), FASTA scores: opt: 776, E(): 1.9e-41, (50.9% identity in 228 aa overlap).; Possible acyltransferase 4281571 886152 Rv3816c Mycobacterium tuberculosis H37Rv Possible acyltransferase NP_218333.1 4280792 R 83332 CDS NP_218334.1 15610953 886144 4281647..4282402 1 NC_000962.3 Rv3817, (MTCY409.13c), len: 251 aa. Possible phosphotransferase, similar to many phosphotransferases e.g. O53023 kanamycin marker from Escherichia coli (264 aa), FASTA scores: opt: 232, E(): 7.5e-08, (32.4% identity in 247 aa overlap); BAA78209|NEO neomycine phosphotransferase from Drosophila melanogaster (Fruit fly) (264 aa), FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); AAG09774 aminoglycoside 3'-phosphotransferase from Vibrio cholerae (264 aa), FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); P00552|KKA2_KLEPN|NEO|KAN aminoglycoside 3'-phosphotransferase from Klebsiella pneumoniae (264 aa),FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); etc.; Possible phosphotransferase 4282402 886144 Rv3817 Mycobacterium tuberculosis H37Rv Possible phosphotransferase NP_218334.1 4281647 D 83332 CDS NP_218335.1 15610954 886153 4282449..4283999 1 NC_000962.3 Rv3818, (MTCY409.12c), len: 516 aa. Unknown protein.; hypothetical protein 4283999 886153 Rv3818 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218335.1 4282449 D 83332 CDS NP_218336.1 15610955 886146 4283996..4284331 1 NC_000962.3 Rv3819, (MTCY409.11c), len: 111 aa. Unknown protein. Contains PS00012 Phosphopantetheine attachment site.; hypothetical protein 4284331 886146 Rv3819 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218336.1 4283996 D 83332 CDS YP_178020.1 57117162 886140 complement(4284419..4285825) 1 NC_000962.3 Rv3820c, (MTCY409.10), len: 468 aa. Possible papA2,conserved polyketide synthase (PKS) associated protein,highly similar to Q49618|PAPA3|ML1230|B1170_C1_180 PKS-associated protein A3 from Mycobacterium leprae (471 aa), FASTA scores: opt: 1660, E(): 2.7e-102, (53.95% identity in 456 aa overlap). Also similar to Q9F2R3|SCD65.19c hypothetical 52.8 KDA protein from Streptomyces coelicolor (473 aa), FASTA scores: opt: 575,E(): 1.8e-30, (27.8% identity in 464 aa overlap); and weakly similar to part of other proteins. Also high similarity with other PKS-associated proteins from Mycobacterium tuberculosis; O50438|PAPA3|Rv1182|MTV005.18 (472 aa), FASTA scores: opt: 1694, E(): 1.5e-104, (53.8% identity in 461 aa overlap); and O07799|PAPA1|Rv3824c|MTCY409.06 (511 aa), FASTA scores: opt: 1664, E(): 1.6e-102, (53.9% identity in 462 aa overlap); and similar to C-terminal end of O53902|PAPA4|Rv1528c|MTV045.02 (165 aa), FASTA scores: opt: 186, E(): 4.1e-05, (37.9% identity in 66 aa overlap).; Possible conserved polyketide synthase associated protein PapA2 4285825 papA2 886140 papA2 Mycobacterium tuberculosis H37Rv Possible conserved polyketide synthase associated protein PapA2 YP_178020.1 4284419 R 83332 CDS NP_218338.1 15610957 886141 4285973..4286686 1 NC_000962.3 Rv3821, (MTCY409.09c), len: 237 aa. Probable conserved integral membrane protein, equivalent to Q49630|ML1233|B1170_F2_64 hypothetical 24.4 KDA protein/INTEGRAL MEMBRANE PROTEIN (POTENTIAL) from Mycobacterium leprae (230 aa), FASTA scores: opt: 619, E(): 2.4e-32, (46.65% identity in 240 aa overlap). Shows some similarity to P29466|I1BC_HUMAN|CASP1|IL1BC|IL1BCE (404 aa), FASTA scores: opt: 126, E(): 0.88, (29.05% identity in 155 aa overlap). Also highly similar to P71796|Rv1517|MTCY277.39 HYPOTHETICAL 26.9 KDA PROTEIN from Mycobacterium tuberculosis (254 aa), FASTA scores: opt: 284, E(): 5.4e-11, (36.35% identity in 256 aa overlap). Start site chosen on basis of similarity to LEPB1170_F2_64 and MTCY277.39, but may extend further upstream. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved integral membrane protein 4286686 886141 Rv3821 Mycobacterium tuberculosis H37Rv Probable conserved integral membrane protein NP_218338.1 4285973 D 83332 CDS NP_218339.1 15610958 886155 4286721..4287935 1 NC_000962.3 Rv3822, (MTCY409.08c), len: 404 aa. Conserved hypothetical protein, similar in part to hypothetical proteins from Mycobacterium leprae: Q9CC62|ML1232 (358 aa) FASTA scores: opt: 601, E(): 1.1e-25, (36.7% identity in 335 aa overlap); and Q49633|B1170_F3_112 (391 aa) FASTA scores: opt: 601, E(): 1.2e-25, (36.25% identity in 347 aa overlap). Also similar to P71862|Rv3539|MTCY03C7.17c PPE family protein from Mycobacterium tuberculosis (479 aa),FASTA scores: opt: 547, E(): 1.3e-22, (38.1% identity in 281 aa overlap); O50440|Rv1184c|MTV005.20c (359 aa); O06828|Rv1430|MTCY493.24c (528 aa); O53642|Rv0159c|MTV032.02c (468 aa); etc.; hypothetical protein 4287935 886155 Rv3822 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218339.1 4286721 D 83332 CDS NP_218340.1 15610959 886145 complement(4288260..4291529) 1 NC_000962.3 Rv3823c, (MTCY409.07), len: 1089 aa. mmpL8,conserved integral membrane transport protein (see Tekaia et al., 1999), member of RND superfamily, equivalent to Q49619|MMLA_MYCLE|MMPL10|TP1|ML1231|B1170_C1_181 putative membrane protein from Mycobacterium leprae (1008 aa), FASTA scores: opt: 2718, E(): 7.3e-149, (56.25% identity in 1028 aa overlap). Also similar to others e.g. Q9XCF6|TMTPC from Mycobacterium avium (974 aa), FASTA scores: opt: 660, E(): 2.7e-30, (28.2% identity in 1050 aa overlap); Q9XCF5|TMTPB from Mycobacterium avium (963 aa), FASTA scores: opt: 653,E(): 6.7e-30, (27.0% identity in 1014 aa overlap); Q9KH53|TMTPC from Mycobacterium smegmatis (994 aa), FASTA scores: opt: 648, E(): 1.3e-29, (28.45% identity in 1013 aa overlap); etc. Also highly similar to other mmpL proteins from Mycobacterium tuberculosis; O50439|MMLA_MYCTU|MMPL10|RV1183|MT1220|MTV005.19 (1002 aa),FASTA scores: opt: 2777, E(): 2.9e-152, (58.25% identity in 996 aa overlap); Q50585|MMLC_MYCTU|MMPL12|Rv1522c|MT1573|MTCY19G5.06 (1146 aa), FASTA scores: opt: 2433, E(): 2.1e-132, (49.9% identity in 1050 aa overlap); and similar to others e.g. P95235|MML9_MYCTU|MMPL9|Rv2339|MT2402|MTCY98.08 (962 aa),FASTA scores: opt: 651, E(): 8.8e-30, (28.6% identity in 1038 aa overlap); etc. Belongs to the MmpL family.; Conserved integral membrane transport protein MmpL8 4291529 mmpL8 886145 mmpL8 Mycobacterium tuberculosis H37Rv Conserved integral membrane transport protein MmpL8 NP_218340.1 4288260 R 83332 CDS NP_218341.1 15610960 886156 complement(4291639..4293174) 1 NC_000962.3 Rv3824c, (MTCY409.06), len: 511 aa. papA1, conserved polyketide synthase (PKS) associated protein, highly similar to Q49618|PAPA3|ML1230|B1170_C1_180 PKS-associated protein A3 from Mycobacterium leprae (471 aa), FASTA scores: opt: 1879, E(): 7.1e-111, (55.5% identity in 465 aa overlap). Also similar to Q9F2R3|SCD65.19c hypothetical 52.8 KDA protein from Streptomyces coelicolor (473 aa),FASTA scores: opt: 476, E(): 1.7e-22, (26.7% identity in 464 aa overlap); and similar in part to Q09164|SIMA|CYSYN cyclosporin synthetase from Tolypocladium inflatum (15281 aa) FASTA scores: opt: 238, E(): 2.8e-06, (22.35% identity in 371 aa overlap). Also highly similar to other PKS-associated proteins from Mycobacterium tuberculosis; O50438|PAPA3|Rv1182|MTV005.18 (472 aa), FASTA scores: opt: 1862, E(): 8.4e-110, (55.95% identity in 470 aa overlap); and upstream ORF O07803|PAPA2|Rv3820c|MTCY409.10 (468 aa) FASTA scores: opt: 1664, E(): 2.5e-97, (53.9% identity in 462 aa overlap). Contains PS00453 FKBP-type peptidyl-prolyl cis-trans isomerase signature 1.; Conserved polyketide synthase associated protein PapA1 4293174 papA1 886156 papA1 Mycobacterium tuberculosis H37Rv Conserved polyketide synthase associated protein PapA1 NP_218341.1 4291639 R 83332 CDS NP_218342.1 15610961 886148 complement(4293225..4299605) 1 NC_000962.3 Rv3825c, (MTCY409.05), len: 2126 aa. pks2,polyketide synthase (see citation below), equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 6828,E(): 0, (63.3% identity in 2128 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase from Mycobacterium leprae (2118 aa) FASTA scores: opt: 5220, E(): 0, (62.4% identity in 2130 aa overlap); or similar in part to others from Mycobacterium leprae e.g. Q9CB70|ML2354 polyketide synthase (1822 aa) FASTA scores: opt: 2787, E(): 2.1e-145, (34.7% identity in 2135 aa overlap). Also highly similar to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 3495,E(): 2.6e-184, (61.65% identity in 2130 aa overlap). Also highly similar to other polyketide synthases from Mycobacterium tuberculosis e.g. O53901|PKS5|Rv1527c|MTV045.01c|MTCY19G5.01 (2108 aa) FASTA scores: opt: 9576, E(): 0, (69.8% identity in 2124 aa overlap); P96291|mas|Rv2940c|MTCY24G1.09|MTCY19H9.08c (2111 aa), FASTA scores: opt: 3518, E(): 1.4e-185, (64.05% identity in 2126 aa overlap); O50437|PKS4|Rv1181|MTV005.17 (1582 aa), FASTA scores: opt: 3461, E(): 1.6e-182, (64.55% identity in 1609 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site and PS00012 Phosphopantetheine attachment site.; Polyketide synthase Pks2 4299605 pks2 886148 pks2 Mycobacterium tuberculosis H37Rv Polyketide synthase Pks2 NP_218342.1 4293225 R 83332 CDS NP_218343.1 15610962 886154 4299812..4301566 1 NC_000962.3 Rv3826, (MTCY409.04c), len: 584 aa. Probable fadD23,fatty-acid-AMP synthetase, highly similar to P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2571, E(): 4.4e-146, (66.15% identity in 576 aa overlap); Q9CD79|FADD28|ML0138 acyl-CoA synthetase from Mycobacterium leprae (579 aa) FASTA scores: opt: 2520, E(): 4.9e-143, (65.2% identity in 575 aa overlap); P54200|FD21_MYCLE putative fatty-acid--CoA ligase (acyl-CoA synthetase) from Mycobacterium leprae (579 aa), FASTA scores: opt: 2330, E(): 1.1e-131, (60.2% identity in 578 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P96290|FADD28|Rv2941|MTCY24G1.08c (580 aa), FASTA scores: opt: 2587, E(): 4.9e-147, (66.5% identity in 576 aa overlap); O53903|FADD24|Rv1529|MTV045.03 (584 aa), FASTA scores: opt: 2457, E(): 2.9e-139, (63.35% identity in 584 aa overlap); Q50586|FADD25|Rv1521|MT1572|MTCY19G5.07 (583 aa) FASTA scores: opt: 2389, E(): 3.3e-135, (61.45% identity in 581 aa overlap); etc.; Probable fatty-acid-AMP ligase FadD23 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 4301566 fadD23 886154 fadD23 Mycobacterium tuberculosis H37Rv Probable fatty-acid-AMP ligase FadD23 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) NP_218343.1 4299812 D 83332 CDS NP_218344.1 15610963 886151 complement(4301563..4302789) 1 NC_000962.3 Rv3827c, (MTCY409.03), len: 408 aa. Possible transposase within IS1537 element, similar to several transposases e.g. O83029|TNPC|DR2324|DR0666|DR0978|DR1381|DR1651|DR1933 transposase from Deinococcus radiodurans(408 aa) FASTA scores: opt: 302, E(): 3.9e-12, (30.75% identity in 358 aa overlap); Q9RXX7|DR0178 putative transposase from Deinococcus radiodurans (409 aa), FASTA scores: opt: 297,E(): 8.2e-12, (31.1% identity in 360 aa overlap); P73816|SLR2062 transposase from Synechocystis sp. strain PCC 6803 (400 aa), FASTA scores: opt: 296, E(): 9.3e-12,(30.05% identity in 353 aa overlap); etc. Highly similar to proteins from Mycobacterium tuberculosis e.g. O33333|Rv2791c|MTV002.56c transposase (459 aa) FASTA scores: opt: 2211, E(): 9.4e-136, (87.75% identity in 367 aa overlap); P95117|Rv2978c|MTCY349.09 hypothetical 51.4 KDA protein (459 aa), FASTA scores: opt: 2165, E(): 9e-133,(85.85% identity in 367 aa overlap); Q10809|YS85_MYCTU|Rv2885c|MT2953|MTCY274.16c hypothetical 51.3 KDA protein (460 aa), FASTA scores: opt: 2127, E(): 2.6e-130, (83.95% identity in 368 aa overlap); O0777|Rv0606|MTCY19H5.16c probable transposase (fragment) (247 aa), FASTA scores: opt: 1405, E(): 9.3e-84, (85.3% identity in 238 aa overlap); etc.; Possible transposase 4302789 886151 Rv3827c Mycobacterium tuberculosis H37Rv Possible transposase NP_218344.1 4301563 R 83332 CDS NP_218345.1 15610964 886160 complement(4302786..4303397) 1 NC_000962.3 Rv3828c, (MTCY409.02), len: 203 aa. Possible resolvase within IS1537 element, similar to others e.g. Q97X40|SSO1915 first ORF in transposon ISC1913 from Sulfolobus solfataricus (213 aa), FASTA scores: opt: 275,E(): 1.6e-11, (30.6% identity in 196 aa overlap); Q9V1M0|PAB2076 resolvase related protein from Pyrococcus abyssi (212 aa), FASTA scores: opt: 254, E(): 4.2e-10,(29.95% identity in 197 aa overlap); Q9RMU7|ORFA putative transposase (belongs to the MerR family of transcriptional regulators) from Helicobacter pylori (Campylobacter pylori) (217 aa), FASTA scores: opt: 243, E(): 2.3e-09, (31.8% identity in 154 aa overlap); etc. Also highly similar to proteins from Mycobacterium tuberculosis e.g. O33334|Rv2792c|MTV002.57c resolvase (193 aa), FASTA scores: opt: 970, E(): 1.5e-58, (79.25% identity in 193 aa overlap); O07773|Rv0605|MTCY19H5.17c putative resolvase (202 aa), FASTA scores: opt: 964, E(): 4e-58, (76.25% identity in 202 aa overlap); P95116|Rv2979c|MTCY349.08 hypothetical 21.4 KDA protein (194 aa), FASTA scores: opt: 895, E(): 1.8e-53, (74.75% identity in 194 aa overlap); Q10831|YS86_MYCTU|Rv2886c|MT2954|MTCY274.17c hypothetical 31.9 KDA protein (295 aa), FASTA scores: opt: 826, E(): 1.1e-48, (66.2% identity in 204 aa overlap) (similarity only at C-terminus); etc. Contains PS00397 Site-specific recombinases active site. Possible helix-turn-helix motif from aa 11-32, Score 1305 (+3.63 SD).; Possible resolvase 4303397 886160 Rv3828c Mycobacterium tuberculosis H37Rv Possible resolvase NP_218345.1 4302786 R 83332 CDS NP_218346.1 15610965 886157 complement(4303398..4305008) 1 NC_000962.3 Rv3829c, (MTCY409.01, MTCY01A6.40), len: 536 aa. Probable oxidoreductase dehydrogenase, similar to others e.g. Q9A3T1|CC3121 phytoene dehydrogenase-related protein from Caulobacter crescentus (543 aa), FASTA scores: opt: 607, E(): 9.2e-28, (28.25% identity in 552 aa overlap); Q98FP6|MLR3676 phytoene dehydrogenase from Rhizobium loti (Mesorhizobium loti) (521 aa), FASTA scores: opt: 605, E(): 1.2e-27, (28.2% identity in 546 aa overlap); Q97W24|SSO2422 phytoene dehydrogenase related protein from Sulfolobus solfataricus (518 aa), FASTA scores: opt: 388, E(): 4.4e-15, (27.35% identity in 530 aa overlap); Q98BS8|MLL5443 probable dehydrogenase from Rhizobium loti (Mesorhizobium loti) (524 aa), FASTA scores: opt: 374, E(): 2.9e-14, (24.35% identity in aa overlap); etc. Also similar to MTCY493.22c|Rv1432|MTCY493.22c hypothetical 50.5 KDA protein (probable dehydrogenase) from Mycobacterium tuberculosis (25.1% identity in 295 aa overlap).; Probable dehydrogenase 4305008 886157 Rv3829c Mycobacterium tuberculosis H37Rv Probable dehydrogenase NP_218346.1 4303398 R 83332 CDS NP_218347.1 15610966 886158 complement(4305056..4305685) 1 NC_000962.3 Rv3830c, (MTCY01A6.39), len: 209 aa. Probable transcriptional regulator TetR family, similar to others e.g. P39885|TCMR_STRGA tetracenomycin C transcriptional repressor from Streptomyces glaucescens (226 aa) FASTA scores: opt: 255, E(): 6.1e-10, (33.65% identity in 202 aa overlap); Q9RDR0|SC4A7.02 putative transcriptional regulator from Streptomyces coelicolor (227 aa) FASTA scores: opt: 230, E(): 2.8e-08, (30.05% identity in 213 aa overlap); Q9EWU3|3SC5B7.06 putative regulatory protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 221,E(): 1.2e-07, (32.05% identity in 181 aa overlap); Q9AJ68|BUTR putative transcriptional repressor from Streptomyces cinnamonensis (268 aa), FASTA scores: opt: 216, E(): 2.7e-07, (37.8% identity in 119 aa overlap); etc. Contains possible helix-turn-helix motif from aa 33-54,Score 1699 (+4.97 SD). Seems to belong to the TetR/AcrR family of transcriptional regulators.; Transcriptional regulatory protein (probably TetR-family) 4305685 886158 Rv3830c Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein (probably TetR-family) NP_218347.1 4305056 R 83332 CDS NP_218348.1 15610967 886161 4305757..4306239 1 NC_000962.3 Rv3831, (MTCY01A6.38c), len: 160 aa. Hypothetical unknown protein.; Hypothetical protein 4306239 886161 Rv3831 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218348.1 4305757 D 83332 CDS NP_218349.1 15610968 886165 complement(4306236..4306811) 1 NC_000962.3 Rv3832c, (MTCY01A6.37), len: 191 aa. Conserved protein, similar in part to various proteins e.g. Q9XBC9|CZA382.22c putative rRNA methylase from Amycolatopsis orientalis (259 aa), FASTA scores: opt: 196,E(): 1.3e-05, (38.2% identity in 110 aa overlap); CAC48459|SMB20059 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (259 aa), FASTA scores: opt: 188, E(): 4.3e-05, (33.8% identity in 136 aa overlap); Q98FP8|MLL3672 methyl transferase-like protein from Rhizobium loti (Mesorhizobium loti) (264 aa), FASTA scores: opt: 180, E(): 0.00014,(32.05% identity in 156 aa overlap); etc.; hypothetical protein 4306811 886165 Rv3832c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218349.1 4306236 R 83332 CDS NP_218350.1 15610969 886162 4306867..4307658 1 NC_000962.3 Rv3833, (MTCY01A6.36c), len: 263 aa. Probable transcriptional regulator belonging to araC family, similar to others e.g. Q9KYN4|SC9H11.05 putative AraC-family transcriptional regulator from Streptomyces coelicolor (289 aa), FASTA scores: opt: 754, E(): 1.2e-42, (50.45% identity in 232 aa overlap); Q9HXH2|PA3830 probable transcriptional regulator from Pseudomonas aeruginosa (270 aa), FASTA scores: opt: 501, E(): 6.2e-26, (34.85% identity in 238 aa overlap); Q9HX87|PA3927 probable transcriptional regulator from Pseudomonas aeruginosa (262 aa), FASTA scores: opt: 496, E(): 1.3e-25, (36.45% identity in 266 aa overlap); P76241|YEAM_ECOLI|B1790 hypothetical transcriptional regulator from Escherichia coli strain K12 (273 aa) FASTA scores: opt: 388, E(): 1.9e-18, (30.5% identity in 223 aa overlap); etc. Contains probable helix-turn-helix motif from aa 164-185, Score 2014 (+6.05 SD). Seems to belong to the AraC/XylS family of transcriptional regulators.; Transcriptional regulatory protein (probably AraC-family) 4307658 886162 Rv3833 Mycobacterium tuberculosis H37Rv Transcriptional regulatory protein (probably AraC-family) NP_218350.1 4306867 D 83332 CDS NP_218351.1 15610970 886163 complement(4307655..4308914) 1 NC_000962.3 Rv3834c, (MTCY01A6.35), len: 419 aa. Probable serS,seryl-tRNA synthetase, equivalent to Q9CDC1|SERS|ML0082 putative SERYL-tRNA synthase from Mycobacterium leprae (417 aa), FASTA scores: opt: 2361, E(): 8.5e-138, (85.8% identity in 416 aa overlap). Also highly similar many e.g. Q9ZBX1|SYS_STRCO|SERS|SCD78.28c from Streptomyces coelicolor (425 aa), FASTA scores: opt: 1594, E(): 1.2e-90,(59.75% identity in 425 aa overlap); Q9X199|SYS_THEMA|SERS|TM1379 from Thermotoga maritima (425 aa), FASTA scores: opt: 1083, E(): 3.3e-59, (43.3% identity in 425 aa overlap); P37464|SYS_BACSU|SERS from Bacillus subtilis (425 aa), FASTA scores: opt: 1015, E(): 5e-55,(39.3% identity in 425 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family.; SERYL-tRNA synthetase SerS (serine--tRNA ligase) (SERRS) (serine translase) 4308914 serS 886163 serS Mycobacterium tuberculosis H37Rv SERYL-tRNA synthetase SerS (serine--tRNA ligase) (SERRS) (serine translase) NP_218351.1 4307655 R 83332 CDS NP_218352.1 15610971 886168 4309047..4310396 1 NC_000962.3 Rv3835, (MTCY01A6.34c), len: 449 aa. Conserved membrane protein, equivalent to Q9CDC2|ML0081 putative membrane protein from Mycobacterium leprae (450 aa), FASTA scores: opt: 2079, E(): 1.8e-74, (69.35% identity in 457 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 4310396 886168 Rv3835 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218352.1 4309047 D 83332 CDS NP_218353.1 15610972 886164 4310401..4310814 1 NC_000962.3 Rv3836, (MTCY01A6.33c), len: 137 aa. Conserved hypothetical protein, highly similar to Q9RKJ2|SCD25.30 hypothetical 13.1 KDA protein from Streptomyces coelicolor (116 aa), FASTA scores: opt: 395, E(): 3.3e-19, (54.4% identity in 114 aa overlap); and similar to CAC47753|SMC0379 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (144 aa) FASTA scores: opt: 194, E(): 6e-06, (33.05% identity in 109 aa overlap); and Q98E37|MLL4425 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (201 aa), FASTA scores: opt: 184, E(): 3.7e-05, (29.75% identity in 121 aa overlap). Contains PS00142 Neutral zinc metallopeptidases,zinc-binding region signature.; hypothetical protein 4310814 886164 Rv3836 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218353.1 4310401 D 83332 CDS NP_218354.1 15610973 886169 complement(4311009..4311707) 1 NC_000962.3 Rv3837c, (MTCY01A6.32), len: 232 aa. Probable phosphoglycerate mutase, equivalent to Q9CDC3|ML0079 putative phosphoglycerate mutase from Mycobacterium leprae (231 aa), FASTA scores: opt: 1116, E(): 7.3e-66, (71.55% identity in 232 aa overlap). Also similar to others e.g. Q9ZAX0|PGM 2,3-PDG dependent phosphoglycerate mutase from Amycolatopsis methanolica (205 aa), FASTA scores: opt: 474,E(): 6.4e-24, (41.85% identity in 203 aa overlap); Q9F3Q7|SC10F4.03 putative isomerase from Streptomyces coelicolor (224 aa) FASTA scores: opt: 349, E(): 1e-15,(33.2% identity in 223 aa overlap); Q9RDL0|SCC123.14c putative phosphoglycerate mutase from Streptomyces coelicolor (223 aa), FASTA scores: opt: 256, E(): 1.2e-09,(34.0% identity in 203 aa overlap); Q9RVD2|DR1097 putative phosphoglycerate mutase from Deinococcus radiodurans (232 aa), FASTA scores: opt: 201, E(): 5.1e-06, (31.45% identity in 175 aa overlap); etc. Also similar to P71724|Rv2419c|MTCY428.28|MTCY253.01 hypothetical 24.2 KDA protein from Mycobacterium tuberculosis (223 aa), FASTA scores: opt: 210, E(): 1.3e-06, (32.0% identity in 172 aa overlap). Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature.; Probable phosphoglycerate mutase (phosphoglyceromutase) (phosphoglycerate phosphomutase) 4311707 886169 Rv3837c Mycobacterium tuberculosis H37Rv Probable phosphoglycerate mutase (phosphoglyceromutase) (phosphoglycerate phosphomutase) NP_218354.1 4311009 R 83332 CDS NP_218355.1 15610974 886170 complement(4311704..4312669) 1 NC_000962.3 Rv3838c, (MTCY01A6.31), len: 321 aa. PheA,prephenate dehydratase (see citation below), equivalent to Q9CDC4|PHEA|ML0078 putative prephenate dehydratase from Mycobacterium leprae (322 aa), FASTA scores: opt: 1690,E(): 1.3e-93, (84.25% identity in 311 aa overlap). Also highly similar to others e.g. P10341|PHEA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (315 aa), FASTA scores: opt: 843, E(): 4e-43, (45.8% identity in 308 aa overlap); Q9ZBX0|SCD78.29c from Streptomyces coelicolor (310 aa), FASTA scores: opt: 820, E(): 9.2e-42,(46.45% identity in 312 aa overlap); Q44104|PHEA_AMYME|PDT from Amycolatopsis methanolica (304 aa), FASTA scores: opt: 707, E(): 4.9e-35, (45.7% identity in 313 aa overlap); etc. Contains PS00858 Prephenate dehydratase signature 2.; Prephenate dehydratase PheA 4312669 pheA 886170 pheA Mycobacterium tuberculosis H37Rv Prephenate dehydratase PheA NP_218355.1 4311704 R 83332 CDS NP_218356.1 15610975 886171 4312765..4313541 1 NC_000962.3 Rv3839, (MTCY01A6.30c), len: 258 aa. Conserved hypothetical protein, similar in part to Q9RD78|SCF43.10cfrom hypothetical 25.8 KDA protein Streptomyces coelicolor (241 aa), FASTA scores: opt: 270,E(): 3.2e-10, (33.45% identity in 272 aa overlap); and O00320|F25451_2 hypothetical protein from Homo sapiens (Human) (339 aa), FASTA scores: opt: 126, E(): 0.77,(28.75% identity in 240 aa overlap).; hypothetical protein 4313541 886171 Rv3839 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218356.1 4312765 D 83332 CDS NP_218357.1 15610976 886167 4313567..4313980 1 NC_000962.3 Rv3840, (MTCY01A6.29c), len: 137 aa. Possible transcriptional regulator, highly similar in part to PSR proteins (penicillin binding protein repressors) e.g. Q47828|PSR PSR protein from Enterococcus hirae (293 aa) FASTA scores: opt: 221, E(): 2.2e-07, (41.65% identity in 108 aa overlap); O86213|PSRFM PSRFM protein (fragment) from Enterococcus hirae (171 aa), FASTA scores: opt: 202, E(): 2.4e-06, (40.75% identity in 108 aa overlap); Q47865|PSR penicillin binding protein repressor from Enterococcus hirae (148 aa), FASTA scores: opt: 201, E(): 2.5e-06,(51.65% identity in 60 aa overlap); etc. Also highly similar in part to other transcriptional regulators e.g. BAB57524|MSRR peptide methionine sulfoxide reductase regulator from Staphylococcus aureus subsp. aureus Mu50 (327 aa), FASTA scores: opt: 195, E(): 1.2e-05, (36.7% identity in 109 aa overlap); Q99Q02|MSRR|SA1195 peptide methionine sulfoxide reductase regulator from Staphylococcus aureus subsp. aureus N315, and Staphylococcus aureus (327 aa), FASTA scores: opt: 192,E(): 1.9e-05, (36.7% identity in 109 aa overlap); Q9K6Q8|LYTR|BH3670 attenuator for lytabc and LYTR expression from Bacillus halodurans (304 aa), FASTA scores: opt: 171, E(): 0.00041, (34.5% identity in 113 aa overlap); etc.; Possible transcriptional regulatory protein 4313980 886167 Rv3840 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein NP_218357.1 4313567 D 83332 CDS NP_218358.1 15610977 886176 4314178..4314723 1 NC_000962.3 Rv3841, (MTCY01A6.28c), len: 181 aa. bfrB,bacterioferritin, similar to other ferritin or hypothetical proteins e.g. O26261|MTH158|RSGA ferritin like protein from Methanothermobacter thermautotrophicus (171 aa), FASTA scores: opt: 277, E(): 6.6e-11, (30.1% identity in 166 aa overlap); Q99SZ3|SA1709 hypothetical protein from Staphylococcus aureus subsp. aureus N315 (166 aa), FASTA scores: opt: 275, E(): 8.7e-11, (33.35% identity in 156 aa overlap); Q9X0L2|TM1128 ferritin from Thermotoga maritima (164 aa), FASTA scores: opt: 247, E(): 5.3e-09, (25.65% identity in 156 aa overlap); Q9KDT7|BH1124 ferritin from Bacillus halodurans (169 aa), FASTA scores: opt: 246, E(): 6.3e-09, (28.95% identity in 152 aa overlap); O29424|AF0834 putative ferritin from Archaeoglobus fulgidu (169 aa),FASTA scores: opt: 246, E(): 6.3e-09, (28.95% identity in 152 aa overlap); etc. Also shows similarity with Rv1876|MTCY180.42|BFRA probable bacterioferritin from Mycobacterium tuberculosis (159 aa). Seems belong to the bacterioferritin family.; Bacterioferritin BfrB 4314723 bfrB 886176 bfrB Mycobacterium tuberculosis H37Rv Bacterioferritin BfrB NP_218358.1 4314178 D 83332 CDS NP_218359.1 15610978 886177 complement(4314738..4315562) 1 NC_000962.3 Rv3842c, (MTCY01A6.27), len: 274 aa. Probable glpQ1,glycerophosphoryl diester phosphodiesterase, equivalent to Q9CDC5|GLPQ|ML0074 putative glycerophosphoryl diester phosphodiesterase from Mycobacterium leprae (271 aa), FASTA scores: opt: 1635, E(): 1.9e-100, (88.85% identity in 269 aa overlap). Also highly similar to others e.g. CAC44700|SCBAC25E3.13c putative phosphodiesterase from Streptomyces coelicolor (275 aa), FASTA scores: opt: 413,E(): 5.7e-20, (48.05% identity in 258 aa overlap); P37965|GLPQ_BACSU glycerophosphoryl diester phosphodiesterase from Bacillus subtilis (293 aa), FASTA scores: opt: 405, E(): 2e-19, (31.3% identity in 249 aa overlap); Q99VC9|GLPQ|SA0820 glycerophosphoryl diester phosphodiesterase from Staphylococcus aureus subsp. aureus N315 (309 aa) FASTA scores: opt: 341, E(): 3.5e-15, (29.3% identity in 273 aa overlap); etc.; Probable glycerophosphoryl diester phosphodiesterase GlpQ1 (glycerophosphodiester phosphodiesterase) 4315562 glpQ1 886177 glpQ1 Mycobacterium tuberculosis H37Rv Probable glycerophosphoryl diester phosphodiesterase GlpQ1 (glycerophosphodiester phosphodiesterase) NP_218359.1 4314738 R 83332 CDS NP_218360.1 15610979 886178 complement(4315568..4316596) 1 NC_000962.3 Rv3843c, (MTCY01A6.26), len: 342 aa. Probable conserved transmembrane protein, equivalent to Q9CDC6|ML0073 putative membrane protein from Mycobacterium leprae (344 aa), FASTA scores: opt: 1420, E(): 2.6e-68,(63.05% identity in 349 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Probable conserved transmembrane protein 4316596 886178 Rv3843c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218360.1 4315568 R 83332 CDS NP_218361.1 15610980 886180 4318775..4319266 1 NC_000962.3 Rv3844, (MTCY01A6.25), len: 163 aa. Possible transposase, identical to P96234|Rv3348|MTV004.04 putative transposase from Mycobacterium tuberculosis. Also some similarity with others e.g. N-terminal part of P19834|YI11_STRCL insertion element IS116 hypothetical 44.8 KDA protein from Streptomyces clavuligerus (399 aa) FASTA scores: opt: 146, E(): 0.017, (29.1% identity in 158 aa overlap).; Possible transposase 4319266 886180 Rv3844 Mycobacterium tuberculosis H37Rv Possible transposase NP_218361.1 4318775 D 83332 CDS NP_218362.1 15610981 886179 4319281..4319640 1 NC_000962.3 Rv3845, (MTCY01A6.24c), len: 119 aa. Hypothetical unknown protein. Contains PS01137 Hypothetical YBL055c/yjjV family signature 1.; Hypothetical protein 4319640 886179 Rv3845 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218362.1 4319281 D 83332 CDS NP_218363.1 15610982 886174 4320704..4321327 1 NC_000962.3 Rv3846, (MTCY01A6.22c), len: 207 aa. SodA (alternate gene names: sodB, sod), superoxide dismutase (see citations below), equivalent to many e.g. P47201|SODM_MYCAV|soda|sod from Mycobacterium avium (206 aa), FASTA scores: opt: 1210,E(): 1.8e-73, (82.5% identity in 206 aa overlap); Q9F9R1|sod from Mycobacterium paratuberculosis (207 aa),FASTA scores: opt: 1207, E(): 2.9e-73, (81.65% identity in 207 aa overlap); O86165|SODM_MYCLP|soda|sod from Mycobacterium lepraemurium (206 aa), FASTA scores: opt: 1204, E(): 4.5e-73, (82.05% identity in 206 aa overlap); P13367|SODM_MYCLE|soda|ML0072 from Mycobacterium leprae (206 aa), FASTA scores: opt: 1169, E(): 9.6e-71, (80.5% identity in 205 aa overlap); etc. Contains PS00088 Manganese and iron superoxide dismutases signature. Belongs to the iron/manganese superoxide dismutase family. Although found extracellularly, no signal sequence is present. An alternative secretory pathway may be used.; Superoxide dismutase [FE] SodA 4321327 sodA 886174 sodA Mycobacterium tuberculosis H37Rv Superoxide dismutase [FE] SodA NP_218363.1 4320704 D 83332 CDS NP_218364.1 15610983 886182 4321538..4322071 1 NC_000962.3 Rv3847, (MTCY01A6.21c), len: 177 aa. Conserved hypothetical protein, equivalent to Q9CDC7|ML0071 hypothetical protein from Mycobacterium leprae (177 aa) FASTA scores: opt: 1149, E(): 1.6e-64, (96.6% identity in 177 aa overlap); and Q9F9R0 hypothetical 18.5 KDA protein from Mycobacterium paratuberculosis (177 aa), FASTA scores: opt: 1139, E(): 6.8e-64, (96.6% identity in 177 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; Hypothetical protein 4322071 886182 Rv3847 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218364.1 4321538 D 83332 CDS NP_218365.1 15610984 886159 4322326..4323234 1 NC_000962.3 Rv3848, (MTCY01A6.20c), len: 302 aa. Probable conserved transmembrane protein, similar to hypothetical (transmembrane) proteins e.g. Q9HVG2|PA4629 hypothetical protein from Pseudomonas aeruginosa (192 aa), FASTA scores: opt: 304, E(): 5.3e-11, (35.05% identity in 174 aa overlap); Q9A5S7|CC2370 hypothetical protein from Caulobacter crescentus (207 aa), FASTA scores: opt: 285,E(): 7.4e-10, (29.9% identity in 184 aa overlap); Q9KY43|SCC8A.05c putative integral membrane protein from Streptomyces coelicolor (193 aa), FASTA scores: opt: 245,E(): 1.6e-07, (32.8% identity in 195 aa overlap); etc.; Probable conserved transmembrane protein 4323234 886159 Rv3848 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218365.1 4322326 D 83332 CDS NP_218366.1 15610985 886184 4323499..4323897 1 NC_000962.3 Rv3849, (MTCY01A6.19c), len: 132 aa. EspR, ESX-1 secreted protein regulator (See Raghavan et al., 2008),equivalent to Q9CDC9|ML0069 hypothetical protein from Mycobacterium leprae (132 aa) FASTA scores: opt: 724, E(): 8.7e-41, (83.95% identity in 131 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX-1 transcriptional regulatory protein EspR 4323897 espR 886184 espR Mycobacterium tuberculosis H37Rv ESX-1 transcriptional regulatory protein EspR NP_218366.1 4323499 D 83332 CDS NP_218367.1 15610986 886173 4324015..4324671 1 NC_000962.3 Rv3850, (MTCY01A6.18c), len: 218 aa. Conserved protein, equivalent to Q9CDD0|ML0068 hypothetical protein from Mycobacterium leprae (238 aa) FASTA scores: opt: 1071,E(): 7.2e-55, (78.35% identity in 217 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; hypothetical protein 4324671 886173 Rv3850 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218367.1 4324015 D 83332 CDS NP_218368.1 15610987 886186 4324683..4324967 1 NC_000962.3 Rv3851, (MTCY01A6.17c), len: 94 aa. Possible membrane protein.; Possible membrane protein 4324967 886186 Rv3851 Mycobacterium tuberculosis H37Rv Possible membrane protein NP_218368.1 4324683 D 83332 CDS NP_218369.1 15610988 886187 4325074..4325478 1 NC_000962.3 Rv3852, (MTCY01A6.16c), len: 134 aa. Possible hns,histone-like protein, equivalent to Q9CDD1|HNS|ML0067 histone-like protein from Mycobacterium leprae (121 aa),FASTA scores: opt: 341, E(): 4.3e-09, (51.5% identity in 134 aa overlap). Shows some similarity with other histone-like proteins e.g. O65795|HIS1 histone H1 from Triticum aestivum (Wheat) (288 aa), FASTA scores: opt: 183,E(): 0.091, (34.85% identity in 109 aa overlap); etc.; Possible histone-like protein Hns 4325478 hns 886187 hns Mycobacterium tuberculosis H37Rv Possible histone-like protein Hns NP_218369.1 4325074 D 83332 CDS NP_218370.1 15610989 886181 4325495..4325968 1 NC_000962.3 Rv3853, (MTCY01A6.15c), len: 157 aa. RraA, regulator of RNase E activity A, equivalent to Q9CDD2|RRAA|ML0066 rraA, regulator of RNase E activity a from Mycobacterium leprae (157 aa) FASTA scores: opt: 896, E(): 1.3e-49,(87.1% identity in 155 aa overlap). Also similar to others e.g. P32165|RRAA_ECOLI|B3929|Z5476|ECS4856 from Escherichia coli strain K12 (161 aa), FASTA scores: opt: 428, E(): 3.7e-20, (45.65% identity in 149 aa overlap); etc. Previously known as menG.; Regulator of RNase E activity a RraA 4325968 rraA 886181 rraA Mycobacterium tuberculosis H37Rv Regulator of RNase E activity a RraA NP_218370.1 4325495 D 83332 CDS NP_218371.1 15610990 886175 complement(4326004..4327473) 1 NC_000962.3 Rv3854c, (MTCY01A6.14), len: 489 aa. EthA (alternate gene names: aka, etaA), monooxygenase required for activation of the pro-drug ethionamide (see citations below), highly similar to other monooxygenases e.g. Q9A588|CC2569 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa), FASTA scores: opt: 1959,E(): 2.9e-114, (57.6% identity in 481 aa overlap); Q9RZT0|DRB0033 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa), FASTA scores: opt: 1771, E(): 2.2e-102, (53.75% identity in 480 aa overlap); Q9A8K5|CC1348 monooxygenase (flavin-binding family) from Caulobacter crescentus (499 aa), FASTA scores: opt: 1385,E(): 1.4e-78, (43.2% identity in 486 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. O53300|Rv3083|MTV013.04 monoxygenase (495 aa) FASTA scores: opt: 1692, E(): 1.1e-97, (49.7% identity in 489 aa overlap); O53762|Rv0565c|MTV039.03c putative monoxygenase (486 aa), FASTA scores: opt: 1571, E(): 3.7e-90, (49.05% identity in 471 aa overlap); O69708|Rv3741c|MTV025.089c possible oxidoreductase (probably second part of a two component monooxygenase) (224 aa), FASTA scores: opt: 542,E(): 1.7e-26, (50.0% identity in 162 aa overlap); etc.; Monooxygenase EthA 4327473 ethA 886175 ethA Mycobacterium tuberculosis H37Rv Monooxygenase EthA NP_218371.1 4326004 R 83332 CDS NP_218372.1 15610991 886189 4327549..4328199 1 NC_000962.3 Rv3855, (MTCY01A6.13c), len: 216 aa. EthR (alternate gene names: aka, etaR), regulatory protein TetR family,involved in ethionamide sensitivity/resistance, negatively controls neighbouring ethA (Rv3854c, MTCY01A6.14; alternate gene names: aka etaA) (see citations below). Equivalent to Q9CDD3|ML0064 putative transcriptional regulator from Mycobacterium leprae (214 aa), FASTA scores: opt: 1017,E(): 7e-62, (77.0% identity in 213 aa overlap). Also similar to other transcriptional regulator e.g. Q9S1R1|SCJ9A.09 putative TetR-family transcriptional regulator from Streptomyces coelicolor (204 aa), FASTA scores: opt: 305, E(): 1.2e-13, (34.5% identity in 200 aa overlap); Q9KYT9|SCE22.24 putative TetR-family transcriptional regulator (fragment) from Streptomyces coelicolor (244 aa), FASTA scores: opt: 179, E(): 4.9e-05,(35.5% identity in 93 aa overlap); Q9RUK2|DR1384 transcriptional regulator (TetR family) from Deinococcus radiodurans (196 aa), FASTA scores: opt: 167, E(): 0.00026,(41.75% identity in 79 aa overlap); etc. Also similar to P95100|Rv3058c|MTCY22D7.23 hypothetical 23.8 KDA protein from Mycobacterium tuberculosis (216 aa) FASTA scores: opt: 261, E(): 1.2e-10, (31.65% identity in 221 aa overlap); and O08377|Rv1534|MTCY07A7A.03 hypothetical 24.5 KDA protein from Mycobacterium tuberculosis (225 aa), FASTA scores: opt: 164, E(): 0.00047, (25.5% identity in 248 aa overlap). Contains helix-turn-helix motif at aa 45-66, Score 1320 (+3.68 SD). Belongs to the TetR/AcrR family of transcriptional regulators.; Transcriptional regulatory repressor protein (TetR-family) EthR 4328199 ethR 886189 ethR Mycobacterium tuberculosis H37Rv Transcriptional regulatory repressor protein (TetR-family) EthR NP_218372.1 4327549 D 83332 CDS NP_218373.1 15610992 886193 complement(4328401..4329408) 1 NC_000962.3 Rv3856c, (MTCY01A6.12), len: 335 aa. Conserved hypothetical protein, highly similar to various proteins from diverse organisms e.g. Q9EWR3|3SCF60.21 conserved hypothetical protein from Streptomyces coelicolor (372 aa) FASTA scores: opt: 1286, E(): 2.4e-73, (64.0% identity in 336 aa overlap); P72464|ORF1 from Streptomyces lividans (343 aa), FASTA scores: opt: 1275, E(): 1.1e-72, (60.1% identity in 336 aa overlap); Q9K899|BH3107 DNA-dependent DNA polymerase beta chain from Bacillus halodurans (571 aa), FASTA scores: opt: 592, E(): 1.2e-29, (39.15% identity in 240 aa overlap); etc. May be a DNA polymerase beta (gene name: yshC) (see citation below).; hypothetical protein 4329408 886193 Rv3856c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218373.1 4328401 R 83332 CDS NP_218374.1 15610993 886192 complement(4329417..4329614) 1 NC_000962.3 Rv3857c, (MTCY01A6.11), len: 65 aa. Possible membrane protein.; Possible membrane protein 4329614 886192 Rv3857c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_218374.1 4329417 R 83332 CDS NP_218375.1 15610994 886196 complement(4330039..4331505) 1 NC_000962.3 Rv3858c, (MTCY01A6.10), len: 488 aa. Probable gltD,small subunit of NADH-dependent glutamate synthase,equivalent to Q9CDD4|GLTD|ML0062 NADH-dependent glutamate synthase small subunit from Mycobacterium leprae (488 aa),FASTA scores: opt: 2997, E(): 1e-166, (87.7% identity in 488 aa overlap). Also highly similar to many e.g. Q9S2Z0|SC3A3.03s from Streptomyces coelicolor (487 aa),FASTA scores: opt: 2152, E(): 1.2e-117, (63.85% identity in 487 aa overlap); Q9KPJ3|VC2374 from Vibrio cholerae (489 aa), FASTA scores: opt: 1699, E(): 2.5e-91, (51.75% identity in 487 aa overlap); Q03460|GLSN_MEDSA from Medicago sativa (Alfalfa) (2194 aa), FASTA scores: opt: 1322, E(): 6.2e-69, (54.45% identity in 485 aa overlap); P09832|GLTD_ECOLI from strain (471 aa) FASTA scores: opt: 889, E() : 0, (37.4% identity in 473 aa overlap); etc. Similar to other glutamate synthases. Cofactor: FAD (by similarity).; Probable NADH-dependent glutamate synthase (small subunit) GltD (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH)) (GLTS beta chain) (NADPH-GOGAT) 4331505 gltD 886196 gltD Mycobacterium tuberculosis H37Rv Probable NADH-dependent glutamate synthase (small subunit) GltD (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH)) (GLTS beta chain) (NADPH-GOGAT) NP_218375.1 4330039 R 83332 CDS NP_218376.1 15610995 886195 complement(4331498..4336081) 1 NC_000962.3 Rv3859c, (MTCY01A6.09), len: 1527 aa. Probable gltB,ferredoxin-dependent glutamate synthase large subunit,equivalent to Q9CDD5|GLTB|ML0061 putative ferredoxin-dependent glutamate synthase from Mycobacterium leprae (1527 aa), FASTA scores: opt: 9277, E(): 0, (90.25% identity in 1527 aa overlap). Also highly similar to many e.g. Q9S2Y9|SC3A3.04c from Streptomyces coelicolor (1514 aa), FASTA scores: opt: 5939, E(): 0, (64.3% identity in 1544 aa overlap); Q9Z465|GLTB from Corynebacterium glutamicum (Brevibacterium flavum) (1510 aa), FASTA scores: opt: 5790, E(): 0, (63.25% identity in 1534 aa overlap); P39812|GLTB_BACSU|GLTA from Bacillus subtilis (1520 aa),FASTA scores: opt: 3445, E(): 2.8e-196, (52.25% identity in 1531 aa overlap); etc. Similar to other glutamate synthases.; Probable ferredoxin-dependent glutamate synthase [NADPH] (large subunit) GltB (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH))(NADPH-GOGAT) 4336081 gltB 886195 gltB Mycobacterium tuberculosis H37Rv Probable ferredoxin-dependent glutamate synthase [NADPH] (large subunit) GltB (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH))(NADPH-GOGAT) NP_218376.1 4331498 R 83332 CDS NP_218377.1 15610996 886188 4336777..4337949 1 NC_000962.3 Rv3860, (MTCY01A6.08c), len: 390 aa. Conserved protein, showing similarity with hypothetical proteins from Mycobacterium leprae e.g. Q9CDD8|ML0048 (586 aa), FASTA scores: opt: 484, E(): 5.5e-14, (29.95% identity in 407 aa overlap); O33082|MLCB628.11c (478 aa) FASTA scores: opt: 484, E(): 4.8e-14, (29.95% identity in 407 aa overlap); etc. Also some similarity with O86637|SC3C3.03c hypothetical 112.1 KDA protein from Streptomyces coelicolor(1083 aa), FASTA scores: opt: 483, E(): 9.6e-14,(30.45% identity in 404 aa overlap). And some similarity with other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O05456|Rv3888c|MTCY15F10.24 hypothetical 37.7 KDA protein (341 aa), FASTA scores: opt: 603, E(): 2.8e-19, (35.2% identity in 284 aa overlap); O06396|Rv0530|MTCY25D10.09 hypothetical 43.0 KDA protein (405 aa), FASTA scores: opt: 538, E(): 2e-16, (31.0% identity in 371 aa overlap); O69740|Rv3876|MTV027.11 (666 aa), FASTA scores: opt: 475,E(): 1.5e-13, (30.2% identity in 391 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop).; hypothetical protein 4337949 886188 Rv3860 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218377.1 4336777 D 83332 CDS NP_218378.1 15610997 886183 4337946..4338272 1 NC_000962.3 Rv3861, (MTCY01A6.07c), len: 108 aa. Hypothetical unknown protein. Overlaps in part next ORF Rv3862c|whiB6.; Hypothetical protein 4338272 886183 Rv3861 Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218378.1 4337946 D 83332 CDS NP_218379.1 15610998 886190 complement(4338171..4338521) 1 NC_000962.3 Rv3862c, (MTCY01A6.06), len: 116 aa. Possible whiB6 (alternate gene name: whmF), WhiB-like regulatory protein (see citation below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Shows similarity with Q49765|WHIB7|ML0639|B1937_F2_68 putative transcriptional regulator WHIB7 from Mycobacterium leprae (89 aa) FASTA scores: opt: 112, E(): 0.49, (41.2% identity in 51 aa overlap). Some similarity to Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa) FASTA scores: opt: 129, E(): 0.038, (32.95% identity in 85 aa overlap); AAK47632|MT3290.1 conserved hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (96 aa), FASTA scores: opt: 126, E(): 0.058,(33.35% identity in 84 aa overlap); Q9FC80|SC4B10.07 conserved hypothetical protein from Streptomyces coelicolor (88 aa), FASTA scores: opt: 119, E(): 0.16, (44.65% identity in 70 aa overlap); Q9K4K8|SC5F8.16c regulatory protein from Streptomyces coelicolor (83 aa), FASTA scores: opt: 114, E(): 0.34, (37.05% identity in 54 aa overlap); etc.; Possible transcriptional regulatory protein WhiB-like WhiB6 4338521 whiB6 886190 whiB6 Mycobacterium tuberculosis H37Rv Possible transcriptional regulatory protein WhiB-like WhiB6 NP_218379.1 4338171 R 83332 CDS NP_218380.1 15610999 886197 4338849..4340027 1 NC_000962.3 Rv3863, (MTCY01A6.05c), len: 392 aa. Unknown ala-rich protein.; Unknown alanine rich protein 4340027 886197 Rv3863 Mycobacterium tuberculosis H37Rv Unknown alanine rich protein NP_218380.1 4338849 D 83332 CDS NP_218381.1 15611000 886185 4340270..4341478 1 NC_000962.3 Rv3864, (MTCY01A6.04c), len: 402 aa. EspE, ESX-1 secretion-associated protein, similar to Q49722|ML0405|B1620_C2_213|MLCL383.01 hypothetical 40.8 KDA protein from Mycobacterium leprae (394 aa) FASTA scores: opt: 397, E(): 1.2e-12, (31.0% identity in 410 aa overlap). Also similar to various proteins from several organisms e.g. Q9VYF9|CG12723 hypothetical protein from Drosophila melanogaster (Fruit fly) (450 aa), FASTA scores: opt: 291,E(): 2.3e-07, (34.6% identity in 130 aa overlap); Q98UE3 procollagen ALPHA1(III) (fragment) from Xenopus laevis (African clawed frog) (117 aa) FASTA scores: opt: 257, E(): 3.6e-06, (41.75% identity in 103 aa overlap); P27393|CA24_ASCSU collagen alpha 2(IV) chain precursor from Ascaris suum (Pig roundworm) (Ascaris lumbricoides) (1763 aa), FASTA scores: opt: 273, E(): 5.7e-06, (32.1% identity in 240 aa overlap); etc. Also similar to O06267|Rv3616c|MTCY07H7B.06 (392 aa) FASTA scores: opt: 389, E(): 3e-12, (31.6% identity in 399 aa overlap).; ESX-1 secretion-associated protein EspE 4341478 espE 886185 espE Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspE NP_218381.1 4340270 D 83332 CDS NP_218382.1 15611001 886172 4341566..4341877 1 NC_000962.3 Rv3865, (MTCY01A6.03c), len: 103 aa. EspF, ESX-1 secretion-associated protein, showing some similarity to O06268|Rv3615c|MTCY07H7B.07 hypothetical 10.8 KDA protein from Mycobacterium tuberculosis (103 aa), FASTA scores: opt: 198, E(): 7.5e-07, (36.25% identity in 102 aa overlap); Q49723|ML0406|B1620_C2_214|MLCL383.02 hypothetical 11.1 KDA protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 154, E(): 0.00071, (31.05% identity in 103 aa overlap).; ESX-1 secretion-associated protein EspF 4341877 espF 886172 espF Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspF NP_218382.1 4341566 D 83332 CDS NP_218383.1 15611002 886200 4341880..4342731 1 NC_000962.3 Rv3866, (MTCY01A6.01c, MTV027.01), len: 283 aa. espG1, ESX-1 secretion-associated protein. N-terminal end highly similar to O33091|MLCB628.20c hypothetical 13.1 KDA protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 260, E(): 2.1e-09, (43.6% identity in 117 aa overlap); and C-terminal end highly similar to O33090|MLCB628.19c hypothetical 36.7 KDA protein from Mycobacterium leprae (338 aa), FASTA scores: opt: 540, E(): 1.4e-26, (54.5% identity in 156 aa overlap). Also similar to Q9CD34|ML2530 possible DNA-binding protein from Mycobacterium leprae (289 aa), FASTA scores: opt: 146, E(): 0.058, (28.25% identity in 269 aa overlap) and O53694|Rv0289|MTV035.17 hypothetical 31.6 KDA protein from Mycobacterium tuberculosis (295 aa),FASTA scores: opt: 133, E(): 0.39, (28.15% identity in 277 aa overlap).; ESX-1 secretion-associated protein EspG1 4342731 espG1 886200 espG1 Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspG1 NP_218383.1 4341880 D 83332 CDS NP_218384.1 15611003 886203 4342770..4343321 1 NC_000962.3 Rv3867, (MTV027.02), len: 183 aa. EspH, ESX-1 secretion-associated protein, highly similar to the hypothetical proteins from Mycobacterium leprae: Q9CDD6|ML0056 (169 aa) FASTA scores: opt: 403, E(): 1.8e-18, (48.2% identity in 166 aa overlap); Q49730|ML0407|B1620_C3_264|MLCL383.03 (216 aa), FASTA scores: opt: 517, E(): 1.7e-25, (51.45% identity in 175 aa overlap); and O33090|MLCB628.19c (338 aa), FASTA scores: opt: 403, E(): 3.4e-18, (48.2% identity in 166 aa overlap). Also highly similar to O06269|Rv3614c|MTCY07H7B.08 hypothetical 19.8 KDA protein from Mycobacterium tuberculosis (184 aa), FASTA scores: opt: 559, E(): 3.4e-28, (54.35% identity in 173 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX-1 secretion-associated protein EspH 4343321 espH 886203 espH Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspH NP_218384.1 4342770 D 83332 CDS NP_218385.1 15611004 886199 4343314..4345035 1 NC_000962.3 Rv3868, (MTV027.03), len: 573 aa. EccA1, esx conserved component, ESX-1 type VII secretion system protein. Member of the CbxX/CfqX family of hypothetical proteins; C-terminal end is highly similar to many e.g. P40118|CBXC_ALCEU|CBXXC|CFXXC CbxX protein (317 aa) FASTA scores: opt: 572, E(): 3e-24, (42.7% identity in 294 aa overlap); CAC48589 probable CBBX protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (311 aa) FASTA scores: opt: 569, E(): 4.3e-24, (40.05% identity in 292 aa overlap); P95648|CBBX_RHOSH CBBX protein from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (309 aa), FASTA scores: opt: 559, E(): 1.5e-23, (41.4% identity in 290 aa overlap); etc. Equivalent to O33089|Y2G8_MYCLE|ML0055|MLCB628.18c hypothetical 62.3 KDA protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 3330, E(): 3.9e-175, (89.2% identity in 573 aa overlap); and similar to Q9CD28|Y282_MYCLE|ML2537 hypothetical 69.1 KDA protein from Mycobacterium leprae (640 aa), FASTA scores: opt: 943, E(): 2.4e-44, (37.5% identity in 571 aa overlap). Also similar to many proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O53687|Y282_MYCTU|Rv0282|MT0295|MTV035.10 hypothetical 68.1 KDA protein (631 aa), FASTA scores: opt: 936, E(): 5.8e-44, (39.05% identity in 568 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop).; ESX conserved component EccA1 ESX-1 type VII secretion system protein 4345035 eccA1 886199 eccA1 Mycobacterium tuberculosis H37Rv ESX conserved component EccA1 ESX-1 type VII secretion system protein NP_218385.1 4343314 D 83332 CDS NP_218386.1 15611005 886166 4345039..4346481 1 NC_000962.3 Rv3869, (MTV027.04), len: 480 aa. EccB1, esx conserved component, ESX-1 type VII secretion system protein, possible membrane protein (has hydrophobic stretch near N-terminus), equivalent to O33088|ML0054|MLCB628.17c putative membrane protein from Mycobacterium leprae (481 aa), FASTA scores: opt: 2489, E(): 8.3e-136, (75.75% identity in 478 aa overlap); and similar to others e.g. Q9Z5I3|ML1544|MLCB596.27 conserved membrane protein from Mycobacterium leprae (506 aa), FASTA scores: opt: 739, E(): 3.9e-35, (33.65% identity in 490 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. O05449|Rv3895c|MTCY15F10.17 (495 aa), FASTA scores: opt: 795, E(): 2.3e-38, (35.8% identity in 486 aa overlap); O53933|Rv1782|MTV049.04 (506 aa), FASTA scores: opt: 763,E(): 1.6e-36, (34.7% identity in 490 aa overlap); O06317|Rv3450c|MTCY13E12.03c (470 aa) FASTA scores: opt: 717, E(): 6.7e-34, (32.55% identity in 479 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX conserved component EccB1 ESX-1 type VII secretion system protein Possible membrane protein 4346481 eccB1 886166 eccB1 Mycobacterium tuberculosis H37Rv ESX conserved component EccB1 ESX-1 type VII secretion system protein Possible membrane protein NP_218386.1 4345039 D 83332 CDS NP_218387.1 15611006 886204 4346481..4348724 1 NC_000962.3 Rv3870, (MTV027.05), len: 747 aa. EccCa1, esx conserved component, ESX-1 type VII secretion system protein, possible transmembrane protein, equivalent to O33087|ML0053|MLCB628.16c putative membrane protein from Mycobacterium leprae (744 aa), FASTA scores: opt: 4333,E(): 0, (85.4% identity in 746 aa overlap); and similar to N-terminal end of others e.g. Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa), FASTA scores: opt: 1003, E(): 1e-52, (33.65% identity in 725 aa overlap); O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 1078, E(): 3e-57,(35.4% identity in 774 aa overlap); P71068|YUKA YUKA protein from Bacillus subtilis (1207 aa) FASTA scores: opt: 529, E(): 4.3e-24, (26.1% identity in 636 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 455, E(): 1.5e-19, (27.1% identity in 734 aa overlap); etc. Also similar to N-terminal end of hypothetical proteins from Mycobacterium tuberculosis e.g. O53689|Rv0284|MTV035.12 (1330 aa), FASTA scores: opt: 982, E(): 1.9e-51, (33.8% identity in 719 aa overlap); O06264|Rv3447c|MTCY77.19c (1236 aa), FASTA scores: opt: 761, E(): 4.1e-38, (38.2% identity in 746 aa overlap); O53935|Rv1784|MTV049.06 (932 aa), FASTA scores: opt: 547, E(): 2.8e-25, (36.25% identity in 276 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Note some similarity (with hypothetical proteins from Mycobacterium tuberculosis and P71068|YUKA) continues in downstream ORF MTV027.06.; ESX conserved component EccCa1 ESX-1 type VII secretion system protein Possible transmembrane protein 4348724 eccCa1 886204 eccCa1 Mycobacterium tuberculosis H37Rv ESX conserved component EccCa1 ESX-1 type VII secretion system protein Possible transmembrane protein NP_218387.1 4346481 D 83332 CDS NP_218388.1 15611007 886202 4348827..4350602 1 NC_000962.3 Rv3871, (MTV027.06), len: 591 aa. EccCb1, esx conserved component, ESX-1 type VII secretion system protein, equivalent to Q9CDD7|ML0052 hypothetical protein from Mycobacterium leprae (597 aa) FASTA scores: opt: 3341,E(): 9.8e-192, (80.85% identity in 596 aa overlap); and O33086|MLCB628.15c hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 3329, E(): 5.1e-191,(80.55% identity in 596 aa overlap). And similar to C-terminal end of others e.g. Q9Z5I2|ML1543|MLCB596.28 possible SPOIIIE-family membrane protein from Mycobacterium leprae (1345 aa), FASTA scores: opt: 601, E(): 5.6e-28,(32.3% identity in 613 aa overlap); O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 977, E(): 2.1e-50, (35.15% identity in 583 aa overlap); Q9L0T6|SCD35.15c putative cell division-related protein from Streptomyces coelicolor (1525 aa), FASTA scores: opt: 414, E(): 9e-17, (27.6% identity in 424 aa overlap);P71068|YUKA YUKA protein from Bacillus subtilis (1207 aa), FASTA scores: opt: 343, E(): 1.3e-12,(25.8% identity in 395 aa overlap); etc. And similar to to C-terminal end of hypothetical proteins from Mycobacterium tuberculosis e.g. O06264|Rv3447c|MTCY77.19c (1236 aa) FASTA scores: opt: 845, E(): 1.5e-42, (35.3% identity in 586 aa overlap); O53689|Rv0284|MTV035.12 (1330 aa) FASTA scores: opt: 646, E(): 1.2e-30, (33.35% identity in 606 aa overlap); O53935|Rv1784|MTV049.06 (932 aa) FASTA scores: opt: 589, E(): 2.1e-27, (33.1% identity in 619 aa overlap); etc. Contains 2 X PS00017 ATP/GTP-binding site motif A (P-loop). Note some similarity (with hypothetical proteins from Mycobacterium tuberculosis and P71068|YUKA) continues in upstream ORF MTV027.05.; ESX conserved component EccCb1 ESX-1 type VII secretion system protein 4350602 eccCb1 886202 eccCb1 Mycobacterium tuberculosis H37Rv ESX conserved component EccCb1 ESX-1 type VII secretion system protein NP_218388.1 4348827 D 83332 CDS YP_178021.1 57117163 886191 4350745..4351044 1 NC_000962.3 Rv3872, (MTV027.07), len: 99 aa. PE35, Some similarity to Mycobacterium tuberculosis conserved PE family proteins (see Brennan & Delogu 2002), e.g. O69713|Rv3746c|MTV025.094c (111 aa), FASTA scores: opt: 306, E(): 5.5e-13, (50.5% identity in 99 aa overlap). Equivalent to AAK48354 from Mycobacterium tuberculosis strain CDC1551 (112 aa) but shorter 14 aa.; PE family-related protein PE35 4351044 PE35 886191 PE35 Mycobacterium tuberculosis H37Rv PE family-related protein PE35 YP_178021.1 4350745 D 83332 CDS YP_178022.1 57117164 886201 4351075..4352181 1 NC_000962.3 Rv3873, (MTV027.08), len: 368 aa. PPE68, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O33085|ML0051|MLCB628.14c from Mycobacterium leprae (302 aa), FASTA scores: opt: 656, E(): 2.8e-24,(46.2% identity in 288 aa overlap); and O53691|Rv0286|MTV035.14 (513 aa), FASTA scores: opt: 566,E(): 7.8e-20, (35.25% identity in 363 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al., 2008).; PPE family protein PPE68 4352181 PPE68 886201 PPE68 Mycobacterium tuberculosis H37Rv PPE family protein PPE68 YP_178022.1 4351075 D 83332 CDS NP_218391.1 15611010 886194 4352274..4352576 1 NC_000962.3 Rv3874, (MT3988, MTV027.09), len: 100 aa. EsxB, 10 KDA culture filtrate antigen (see citations below,especially first), highly similar to O33084|CF10_MYCLE|ML0050|MLCB628.13c 10 KDA culture filtrate antigen CFP10 homolog from Mycobacterium leprae (99 aa), FASTA scores: opt: 237, E(): 2.4e-08, (39.4% identity in 99 aa overlap). Also similar to O05440|ES6D_MYCTU|Rv3905c|MT4024|MTCY15F10.06 putative ESAT-6 like protein 13 from Mycobacterium tuberculosis (103 aa) FASTA scores: opt: 126, E(): 0.18, (23.1% identity in 91 aa overlap); and shows some similarity with other proteins from Mycobacterium tuberculosis. Contains probable coiled-coil from aa 49-93. Belongs to the ESAT6 family. Note that previously known as lhp (alternate gene name: cfp10). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; 10 kDa culture filtrate antigen EsxB (LHP) (CFP10) 4352576 esxB 886194 esxB Mycobacterium tuberculosis H37Rv 10 kDa culture filtrate antigen EsxB (LHP) (CFP10) NP_218391.1 4352274 D 83332 CDS YP_178023.1 57117165 886209 4352609..4352896 1 NC_000962.3 Rv3875, (MT3989, MTV027.10), len: 95 aa. EsxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 early secretory antigenic target from Mycobacterium bovis (94 aa), FASTA scores: opt: 596,E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA early secretory antigenic target homolog (ESAT-6-like protein) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03,(26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. Belongs to the ESAT6 family. Note that previously known as esat-6. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al.,2008). EspD|Rv3614c expression but not secretion is required for EsxA|Rv3875 secretion (See Chen et al.,2012).; 6 kDa early secretory antigenic target EsxA (ESAT-6) 4352896 esxA 886209 esxA Mycobacterium tuberculosis H37Rv 6 kDa early secretory antigenic target EsxA (ESAT-6) YP_178023.1 4352609 D 83332 CDS NP_218393.1 15611012 886206 4353010..4355010 1 NC_000962.3 Rv3876, (MTV027.11), len: 666 aa. EspI, ESX-1 secretion-associated protein, conserved pro-, ala-rich protein, similar to several proteins from Mycobacterium leprae e.g. Q9CDD8|ML0048 hypothetical protein (586 aa),FASTA scores: opt: 1682, E(): 2.1e-45, (50.75% identity in 672 aa overlap); O33082|MLCB628.11c hypothetical 52.0 KDA protein (478 aa), FASTA scores: opt: 1588, E(): 1.5e-42,(53.5% identity in 542 aa overlap) (also has a proline rich N-terminus); etc. Also similar to other proteins from Mycobacterium tuberculosis, especially in C-terminus, e.g. O06396|Rv0530|MTCY25D10.09 (405 aa), FASTA scores: opt: 670, E(): 2.5e-14, (34.85% identity in 396 aa overlap) (also has Pro-rich N-terminus); etc. Note that N-terminus is repetitive and highly Proline rich.; ESX-1 secretion-associated protein EspI Conserved proline and alanine rich protein 4355010 espI 886206 espI Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspI Conserved proline and alanine rich protein NP_218393.1 4353010 D 83332 CDS NP_218394.1 15611013 886207 4355007..4356542 1 NC_000962.3 Rv3877, (MTV027.12), len: 511 aa. EccD1, esx conserved component, ESX-1 type VII secretion system protein, probable transmembrane protein, equivalent to Q9CDD9|ML0047 putative membrane protein from Mycobacterium leprae (512 aa), FASTA scores: opt: 2496, E(): 2.8e-140,(74.0% identity in 512 aa overlap); and highly similar, but longer 32 aa, to O33081|MLCB628.10c hypothetical 51.4 KDA protein from Mycobacterium leprae (480 aa), FASTA scores: opt: 2346, E(): 2e-131, (74.15% identity in 480 aa overlap). Shows also similarity with other membrane proteins from Mycobacterium leprae e.g. Q9CBV2|ML1539 probable membrane protein (503 aa), FASTA scores: opt: 318,E(): 2e-11, (22.7% identity in 520 aa overlap). Also similar to various proteins from Mycobacterium tuberculosis e.g. O53944|Rv1795|MTV049.17 putative membrane protein (503 aa), FASTA scores: opt: 391, E(): 9.4e-16, (24.45% identity in 523 aa overlap); O86362|Rv0290|MTV035.18 hypothetical 47.9 KDA protein (472 aa), FASTA scores: opt: 332, E(): 2.8e-12, (28.1% identity in 509 aa overlap); O05457|Rv3887c|MTCY15F10.25 hypothetical 53.2 KDA protein (509 aa), FASTA scores: opt: 167, E(): 0.017, (24.0% identity in 517 aa overlap); etc. Equivalent to AAK48359 from Mycobacterium tuberculosis strain CDC1551 (479 aa) but longer 32 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX conserved component EccD1 ESX-1 type VII secretion system protein Probable transmembrane protein 4356542 eccD1 886207 eccD1 Mycobacterium tuberculosis H37Rv ESX conserved component EccD1 ESX-1 type VII secretion system protein Probable transmembrane protein NP_218394.1 4355007 D 83332 CDS NP_218395.1 15611014 886198 4356693..4357535 1 NC_000962.3 Rv3878, (MTV027.13), len: 280 aa. EspJ, ESX-1 secretion-associated protein, conserved ala-rich protein. Predicted to be an outer membrane protein (See Song et al.,2008).; ESX-1 secretion-associated protein EspJ Conserved alanine rich protein 4357535 espJ 886198 espJ Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspJ Conserved alanine rich protein NP_218395.1 4356693 D 83332 CDS NP_218396.1 15611015 886212 complement(4357593..4359782) 1 NC_000962.3 Rv3879c, (MTV027.14c), len: 729 aa. EspK, ESX-1 secretion-associated protein, ala- and pro-rich protein (N-terminal end is repetitive and highly Proline-rich). There may be an unknown protein Orf14 encoded in the opposite orientation, within rv3879c (See Ahmad et al.,1999; Daugelat et al., 2003).; ESX-1 secretion-associated protein EspK Alanine and proline rich protein 4359782 espK 886212 espK Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspK Alanine and proline rich protein NP_218396.1 4357593 R 83332 CDS NP_218397.1 15611016 886205 complement(4360199..4360546) 1 NC_000962.3 Rv3880c, (MTV027.15c), len: 115 aa. EspL, ESX-1 secretion-associated protein, equivalent to O33080|ML0044|MLCB628.09 hypothetical 12.2 KDA protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 397, E(): 2e-19, (56.35% identity in 110 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).; ESX-1 secretion-associated protein EspL 4360546 espL 886205 espL Mycobacterium tuberculosis H37Rv ESX-1 secretion-associated protein EspL NP_218397.1 4360199 R 83332 CDS NP_218398.1 15611017 886214 complement(4360543..4361925) 1 NC_000962.3 Rv3881c, (MTV027.16c), len: 460 aa. EspB, ESX-1 substrate protein B (See McLaughlin et al., 2007). Conserved ala-, gly-rich protein. C-terminal end highly similar to O06126 hypothetical 9.5 KDA protein (fragment) from Mycobacterium tuberculosis strain NTI 64719 (90 aa) FASTA scores: opt: 333, E(): 6.3e-07, (69.75% identity in 86 aa overlap) but sequence difference causes frameshift in NTI 64719. Also similar to part of small Mycobacterium leprae ORF O33078|MLCB628.06 (EMBL:Y14967) (101 aa), FASTA scores: opt: 194, E(): 0.04, (59.3% identity in 54 aa overlap), suggesting this is represented by a pseudogene in Mycobacterium leprae.; Secreted ESX-1 substrate protein B, EspB. Conserved alanine and glycine rich protein 4361925 espB 886214 espB Mycobacterium tuberculosis H37Rv Secreted ESX-1 substrate protein B, EspB. Conserved alanine and glycine rich protein NP_218398.1 4360543 R 83332 CDS NP_218399.1 15611018 886208 complement(4362032..4363420) 1 NC_000962.3 Rv3882c, (MTV027.17c, MTCY15F10.30), len: 462 aa. eccE1, esx conserved component, ESX-1 type VII secretion system protein, possible membrane protein, equivalent to O33077|ML0042|MLCB628.05 putative membrane protein from Mycobacterium leprae (467 aa), FASTA scores: opt: 2346,E(): 1.1e-140, (72.1% identity in 462 aa overlap). Also similar to O05459|Rv3885c|MTCY15F10.27 possible membrane protein from Mycobacterium tuberculosis (537 aa) FASTA scores: opt: 283, E(): 2.5e-10, (26.8% identity in 414 aa overlap); and C-terminal end shows similarity with AAK48368|MT4000 hypothetical 45.6 KDA protein from Mycobacterium tuberculosis strain CDC1551 (422 aa) FASTA scores: opt: 215, E(): 4.1e-06, (26.85% identity in 320 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Rv3614c and Rv3882c interact, by yeast two-hybrid analysis (See MacGurn et al., 2005).; ESX conserved component EccE1 ESX-1 type VII secretion system protein Possible membrane protein 4363420 eccE1 886208 eccE1 Mycobacterium tuberculosis H37Rv ESX conserved component EccE1 ESX-1 type VII secretion system protein Possible membrane protein NP_218399.1 4362032 R 83332 CDS NP_218400.1 15611019 886217 complement(4363417..4364757) 1 NC_000962.3 Rv3883c, (MTCY15F10.29), len: 446 aa. MycP1,membrane-anchored serine protease (mycosin) (see citations below), equivalent to O33076|ML0041|MLCB628.04 probable secreted protease from Mycobacterium leprae (446 aa), FASTA scores: opt: 2448, E(): 1.5e-124, (79.15% identity in 446 aa overlap); and highly similar, but in part, to several putative proteases from Mycobacterium leprae; Q9CBV3|ML1538 (567 aa) FASTA scores: opt: 902, E(): 3e-41, (37.25% identity in 556 aa overlap); and Q9CD36|ML2528 (475 aa),FASTA scores: opt: 873, E(): 9.4e-40, (42.7% identity in 459 aa overlap). Shows also similarity with several proteases from other organisms e.g. Q9PCD0|XF1851 serine protease from Xylella fastidiosa (1000 aa), FASTA scores: opt: 281, E(): 1.3e-07, (27.95% identity in 422 aa overlap); P42780|BPRX_BACNO extracellular subtilisin-like protease precursor from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 270, E(): 3.2e-07,(28.9% identity in 384 aa overlap); Q46541|APRV5 acidic protease V5 from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 264, E(): 6.8e-07,(28.65% identity in 384 aa overlap); etc. Also highly similar to various proteins from Mycobacterium tuberculosis e.g. O53695|Rv0291|MTV035.19 probable membrane-anchored mycosin MYCP3 (461 aa), FASTA scores: opt: 1168, E(): 1.2e-55, (44.6% identity in 453 aa overlap); O53945|Rv1796|MTV049.18 probable membrane-anchored mycosin MYCP5 (585 aa), FASTA scores: opt: 928, E(): 1.2e-42,(37.85% identity in 555 aa overlap) (note gap from aa 155-264); and downstream ORF O05458|Rv3886c|MTCY15F10.26 probable membrane-anchored mycosin MYCP2 (550 aa), FASTA scores: opt: 910, E(): 1.1e-41, (40.15% identity in 533 aa overlap) (note partial gap from aa 146-234); etc. Equivalent to AAK48366 from Mycobacterium tuberculosis strain CDC1551 (411 aa) but longer 35 aa. Has signal sequence with possible signal peptidase I cleavage site in residues 19-21 (ASA) and hydrophobic stretch at C-terminus,followed by short positively charged segment, that seems to act as a membrane anchor. Activated by Ca2+ (see Dave et al., 2002). Contains three serine protease, subtilase family active site motifs: a aspartic acid active site motif (PS00136); a histidine active site motif (PS00137); and a serine active site motif (PS00138). Belongs to peptidase family S8 (also known as the subtilase family),pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Membrane-anchored mycosin MycP1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1) 4364757 mycP1 886217 mycP1 Mycobacterium tuberculosis H37Rv Membrane-anchored mycosin MycP1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1) NP_218400.1 4363417 R 83332 CDS NP_218401.1 15611020 886210 complement(4364979..4366838) 1 NC_000962.3 Rv3884c, (MTCY15F10.28), len: 619 aa. eccA2, esx conserved component, ESX-2 type VII secretion system protein. Probable CbxX/CfqX protein family, similar to hypothetical proteins from Mycobacterium leprae e.g. Q9CD28|Y282_MYCLE|ML2537 (640 aa), FASTA scores: opt: 725,E(): 2.9e-34, (28.95% identity in 587 aa overlap); O33089|Y2G8_MYCLE|ML0055|MLCB628.18c (belongs to the CbxX/CfqX family) (573 aa); Q9CBV5|ML1536 (610 aa) FASTA scores: opt: 648, E(): 7.4e-30, (31.5% identity in 549 aa overlap). Also similar to proteins belonging to the CbxX/CfqX family e.g. Q9RKZ2|SC6D7.05c putative CbxX/CfqX family protein from Streptomyces coelicolor (618 aa) FASTA scores: opt: 557, E(): 1.3e-24, (28.6% identity in 601 aa overlap); P27643|SP5K_BACSU|SPOVK|SPOVJ stage V sporulation protein K from Bacillus subtilis (322 aa) FASTA scores: opt: 485, E(): 1.1e-20, (35.0% identity in 280 aa overlap) (similarity only at C-terminus); Q9KAC6|BH2363 stage V sporulation protein K from Bacillus halodurans (315 aa),FASTA scores: opt: 462, E(): 2.2e-19, (36.05% identity in 244 aa overlap) (similarity only at C-terminus); etc. And similar to hypothetical proteins from Mycobacterium tuberculosis belonging to the CbxX/CfqX family e.g. O53687|Y282_MYCTU|Rv0282|MT0295|MTV035.10 hypothetical 68.1 KDA protein (631 aa), FASTA scores: opt: 743, E(): 2.6e-35,(29.9% identity in 612 aa overlap); O69733|Y2G8_MYCTU|Rv3868|MT3981|MTV027.03 hypothetical 62.4 KDA protein (573 aa), FASTA scores: opt: 678, E(): 1.3e-31,(31.25% identity in 589 aa overlap); O53947|YH98_MYCTU|Rv1798|MT1847|MTV049.20 (610 aa) FASTA scores: opt: 669, E(): 4.6e-31, (30.95% identity in 549 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Seems to belong to the CbxX/CfqX family.; ESX conserved component EccA2 ESX-2 type VII secretion system protein Probable CbxX/CfqX family protein 4366838 eccA2 886210 eccA2 Mycobacterium tuberculosis H37Rv ESX conserved component EccA2 ESX-2 type VII secretion system protein Probable CbxX/CfqX family protein NP_218401.1 4364979 R 83332 CDS NP_218402.1 15611021 886220 complement(4366908..4368521) 1 NC_000962.3 Rv3885c, (MTCY15F10.27), len: 537 aa. eccE2, esx conserved component, ESX-2 type VII secretion system protein. possible membrane protein (has hydrophobic stretch near N-terminus), showing some similarity with O05462|Rv3882c|MTV027.17c|MTCY15F10.30 possible membrane protein from Mycobacterium tuberculosis (462 aa) FASTA scores: opt: 283, E(): 8.3e-10, (26.55% identity in 414 aa overlap); and O33077|ML0042|MLCB628.05 putative membrane protein from Mycobacterium leprae (467 aa), FASTA scores: opt: 260, E(): 2.1e-08, (28.0% identity in 382 aa overlap). Equivalent to AAK48368 from Mycobacterium tuberculosis strain CDC1551 (422 aa) but longer 115 aa.; ESX conserved component EccE2 ESX-2 type VII secretion system protein Possible membrane protein 4368521 eccE2 886220 eccE2 Mycobacterium tuberculosis H37Rv ESX conserved component EccE2 ESX-2 type VII secretion system protein Possible membrane protein NP_218402.1 4366908 R 83332 CDS NP_218403.1 15611022 886215 complement(4368518..4370170) 1 NC_000962.3 Rv3886c, (MTCY15F10.26), len: 550 aa. Probable mycP2, ala-, pro-rich membrane-anchored serine protease (mycosin) (see citation below), highly similar to Q9CBV3|ML1538 possible protease from Mycobacterium leprae (567 aa), FASTA scores: opt: 1034, E(): 3.9e-32, (43.5% identity in 575 aa overlap); and highly similar, but with gaps, to several putative proteases from Mycobacterium leprae; O33076|ML0041|MLCB628.04 (446 aa), FASTA scores: opt: 860, E(): 1.1e-25, (38.65% identity in 538 aa overlap); Q9CD36|ML2528 (475 aa) (475 aa), FASTA scores: opt: 413, E(): 7.1e-09, (37.7% identity in 562 aa overlap). Also similarity with Q99405|PRTM_BACSP M-protease from Bacillus sp. strain KSM-K16 (269 aa), FASTA scores: E(): 7.6e-06, (27.1% identity in 277 aa overlap). And highly similar, but also with gaps, to other mycosins from Mycobacterium tuberculosis e.g. O53945|Rv1796|MTV049.18 (585 aa), FASTA scores: opt: 1173, E(): 2.4e-37, (47.9% identity in 578 aa overlap); the upstream ORF O05461|Rv3883c|MTCY15F10.29 (446 aa) FASTA scores: opt: 910, E(): 1.5e-27, (40.15% identity in 533 aa overlap); O06316|Rv3449|MTCY13E12.02 (455 aa) FASTA scores: opt: 477,E(): 2.7e-11, (38.75% identity in 550 aa overlap); etc. Contains Pro rich protein with two serine protease,subtilase family active site motifs: aspartic acid active site motif (PS00136); and histidine active site motif (PS00137). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Thought to be cleaved into smaller molecular weight proteins, 36 and 29 KDA (see citation below).; Probable alanine and proline rich membrane-anchored mycosin MycP2 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-2) 4370170 mycP2 886215 mycP2 Mycobacterium tuberculosis H37Rv Probable alanine and proline rich membrane-anchored mycosin MycP2 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-2) NP_218403.1 4368518 R 83332 CDS NP_218404.1 15611023 886211 complement(4370155..4371684) 1 NC_000962.3 Rv3887c, (MTCY15F10.25), len: 509 aa. eccD2, esx conserved component, ESX-2 type VII secretion system protein, probable transmembrane protein (has hydrophilic stretch from ~1-130 then very hydrophobic domain), similar to other membrane proteins and with weak similarity to known transporters, e.g. Q9CBV2|ML1539 probable membrane protein from Mycobacterium leprae (503 aa), FASTA scores: opt: 395, E(): 2.3e-16, (28.0% identity in 496 aa overlap); Q9CD35|ML2529 conserved membrane protein from Mycobacterium leprae (485 aa), FASTA scores: opt: 221, E(): 6.6e-06,(24.6% identity in 423 aa overlap); Q9ADP8|2SC10A7.11 putative integral membrane protein from Streptomyces coelicolor (430 aa), FASTA scores: opt: 171, E(): 0.0062,(26.55% identity in 358 aa overlap); CAC44275|SCBAC17F8.03 putative drug efflux protein from Streptomyces coelicolor (416 aa), FASTA scores: opt: 160, E(): 0.028, (27.85% identity in 323 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. O53944|Rv1795|MTV049.17 putative membrane protein (503 aa),FASTA scores: opt: 360, E(): 2.9e-14, (26.65% identity in 514 aa overlap); etc. Equivalent to AAK48369 from Mycobacterium tuberculosis strain CDC1551 (469 aa) but longer 40 aa.; ESX conserved component EccD2 ESX-2 type VII secretion system protein Probable transmembrane protein 4371684 eccD2 886211 eccD2 Mycobacterium tuberculosis H37Rv ESX conserved component EccD2 ESX-2 type VII secretion system protein Probable transmembrane protein NP_218404.1 4370155 R 83332 CDS NP_218405.1 15611024 886219 complement(4371681..4372706) 1 NC_000962.3 Rv3888c, (MTCY15F10.24), len: 341 aa. Probable conserved membrane protein, showing similarity with hypothetical proteins from Mycobacterium leprae: O33082|MLCB628.11c (478 aa), FASTA scores: opt: 530, E(): 7.7e-26, (32.45% identity in 336 aa overlap); Q9CDD8|ML0048 (586 aa), FASTA scores: opt: 530, E(): 9.1e-26, (32.45% identity in 336 aa overlap); Q9CCI1|ML0798 (592 aa), FASTA scores: opt: 426, E(): 3e-19, (27.5% identity in 342 aa overlap) (similarity only at C-terminus). Also similar to proteins from Mycobacterium tuberculosis e.g. P96217|Rv3860|MTCY01A6.08c (390 aa), FASTA scores: opt: 603, E(): 1.7e-30, (35.2% identity in 284 aa overlap); O06396|Rv0530|MTCY25D10.09 (405 aa), FASTA scores: opt: 573, E(): 1.3e-28, (32.0% identity in 328 aa overlap); C-terminus of O69740|Rv3876|MTV027.1 (666 aa), FASTA scores: opt: 509, E(): 2.1e-24, (31.0% identity in 303 aa overlap); etc.; Probable conserved membrane protein 4372706 886219 Rv3888c Mycobacterium tuberculosis H37Rv Probable conserved membrane protein NP_218405.1 4371681 R 83332 CDS NP_218406.1 15611025 886223 complement(4372800..4373630) 1 NC_000962.3 Rv3889c, (MTCY15F10.23), len: 276 aa. EspG2, ESX-2 secretion-associated protein.; ESX-2 secretion-associated protein EspG2 4373630 espG2 886223 espG2 Mycobacterium tuberculosis H37Rv ESX-2 secretion-associated protein EspG2 NP_218406.1 4372800 R 83332 CDS NP_218407.1 15611026 886222 complement(4373726..4374013) 1 NC_000962.3 Rv3890c, (MT4005, MTCY15F10.22), len: 95 aa. EsxC,ESAT-6 like protein (see Gey Van Pittius et al., 2001),equivalent to Q9K548|ES6B_MYCPA putative ESAT-6 like protein 11 (ORF3890C) from Mycobacterium paratuberculosis (95 aa), FASTA scores: opt: 490, E(): 3.3e-26, (76.85% identity in 95 aa overlap). Belongs to the ESAT6 family.; ESAT-6 like protein EsxC (ESAT-6 like protein 11) 4374013 esxC 886222 esxC Mycobacterium tuberculosis H37Rv ESAT-6 like protein EsxC (ESAT-6 like protein 11) NP_218407.1 4373726 R 83332 CDS NP_218408.1 15611027 886218 complement(4374049..4374372) 1 NC_000962.3 Rv3891c, (MTCY15F10.21), len: 107 aa (first GTG taken). EsxD, ESAT-6 like protein, equivalent to Q9K547 hypothetical 10.3 KDA protein (fragment) from Mycobacterium paratuberculosis (100 aa), FASTA scores: opt: 498, E(): 1.7e-26, (77.25% identity in 101 aa overlap). Seems to belong to the ESAT6 family (see Gey Van Pittius et al.,2001).; Possible ESAT-6 like protein EsxD 4374372 esxD 886218 esxD Mycobacterium tuberculosis H37Rv Possible ESAT-6 like protein EsxD NP_218408.1 4374049 R 83332 CDS YP_178024.1 57117166 886227 complement(4374484..4375683) 1 NC_000962.3 Rv3892c, (MTCY15F10.20), len: 399 aa. PPE69, Member of the Mycobacterium tuberculosis PPE family of conserved proteins, similar to many e.g. O05298|Rv1196|MTCI364.08 from Mycobacterium leprae (391 aa), FASTA scores: opt: 348,E(): 2.2e-08, (26.6% identity in 380 aa overlap).; PPE family protein PPE69 4375683 PPE69 886227 PPE69 Mycobacterium tuberculosis H37Rv PPE family protein PPE69 YP_178024.1 4374484 R 83332 CDS YP_178025.1 57117167 886213 complement(4375762..4375995) 1 NC_000962.3 Rv3893c, (MTCY15F10.19), len: 77 aa. PE36, Member of the Mycobacterium tuberculosis PE family of conserved proteins (see citation below), similar to others e.g. O53690|Rv0285|MTV035.13 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 136, E(): 0.042, (35.6% identity in 73 aa overlap).; PE family protein PE36 4375995 PE36 886213 PE36 Mycobacterium tuberculosis H37Rv PE family protein PE36 YP_178025.1 4375762 R 83332 CDS NP_218411.1 15611030 886230 complement(4376262..4380452) 1 NC_000962.3 Rv3894c, (MTCY15F10.18), len: 1396 aa. EccC2, esx conserved component, ESX-2 type VII secretion system protein, possible membrane protein (possible transmembrane segments from aa ~37-85), similar to Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa),FASTA scores: opt: 652, E(): 2.2e-30, (27.85% identity in 1425 aa overlap); Q9CDD7|ML0052 hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 537, E(): 6.6e-24, (27.5% identity in 585 aa overlap) (similarity only with C-terminal end); Q9Z5I2|ML1543|MLCB596.28 possible SPOIIIE-family membrane protein from Mycobacterium leprae (1345 aa), FASTA scores: opt: 523, E(): 8.6e-23,(31.65% identity in 1412 aa overlap). Also similar to various proteins e.g. O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 973, E(): 2.8e-49, (28.1% identity in 1409 aa); Q9L0T6|SCD35.15c putative cell division-related protein from Streptomyces coelicolor(1525 aa), FASTA scores: opt: 524, E(): 8.3e-23, (24.95% identity in 1450 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 444, E(): 4.1e-18,(22.5% identity in 1346 aa overlap); etc. Also similar to AAK46103|MT1833 FTSK/SPOIIIE family protein from Mycobacterium tuberculosis strain CDC1551 (1391 aa), FASTA scores: opt: 769, E(): 2.9e-37, (30.6% identity in 1434 aa overlap); and other hypothetical proteins from Mycobacterium tuberculosis e.g. O53689|Rv0284|MTV035.12 (1330 aa), FASTA scores: opt: 634, E(): 2.5e-29, (28.2% identity in 1443 aa overlap); O06264|Rv3447c|MTCY77.19c (1236 aa), FASTA scores: opt: 632, E(): 3.1e-29, (28.75% identity in 1391 aa overlap); O69736|R3871|MTV027.06 (591 aa), FASTA scores: opt: 588, E(): 6.6e-27, (27.75% identity in 605 aa overlap) (similarity only with C-terminal end); etc. Contains two possible (PS00017) ATP/GTP-binding sites (P-loop) in central portion.; ESX conserved component EccC2 ESX-2 type VII secretion system protein Possible membrane protein 4380452 eccC2 886230 eccC2 Mycobacterium tuberculosis H37Rv ESX conserved component EccC2 ESX-2 type VII secretion system protein Possible membrane protein NP_218411.1 4376262 R 83332 CDS NP_218412.1 15611031 886231 complement(4380453..4381940) 1 NC_000962.3 Rv3895c, (MTCY15F10.17), len: 495 aa. EccB2, esx conserved component, ESX-2 type VII secretion system protein, probable membrane protein, highly similar to two conserved membrane protein from Mycobacterium leprae: Q9Z5I3|ML1544|MLCB596.27 (506 aa), FASTA scores: opt: 1070,E(): 1.4e-53, (39.8% identity in 485 aa overlap); and Q9CD29|ML2536 (552 aa), FASTA scores: opt: 483, E(): 4e-20,(36.85% identity in 499 aa overlap). Also highly similar to various proteins from Mycobacterium tuberculosis e.g. O53933|Rv1782|MTV049.04 hypothetical protein (506 aa),FASTA scores: opt: 1106, E(): 1.2e-55, (41.25% identity in 485 aa overlap); O69734|Rv3869|MTV027.04 hypothetical protein (480 aa), FASTA scores: opt: 795, E(): 6.1e-38,(36.0% identity in 486 aa overlap); O33088|ML0054|MLCB628.17c putative membrane protein (481 aa), FASTA scores: opt: 740, E(): 8.3e-35, (35.65% identity in 485 aa overlap); etc.; ESX conserved component EccB2 ESX-2 type VII secretion system protein Probable membrane protein 4381940 eccB2 886231 eccB2 Mycobacterium tuberculosis H37Rv ESX conserved component EccB2 ESX-2 type VII secretion system protein Probable membrane protein NP_218412.1 4380453 R 83332 CDS NP_218413.1 15611032 886216 complement(4381943..4382851) 1 NC_000962.3 Rv3896c, (MTCY15F10.16), len: 302 aa (first GTG taken, although TBParse suggests TTG at 16079). Putative conserved ala-rich protein. C-terminus highly similar to C-terminal end of other proteins e.g. Q9XAS4|SC10A7.01 hypothetical 17.2 KDA protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 255, E(): 1.4e-08, (32.0% identity in 222 aa overlap); CAC44611|STBAC16H6.32 putative secreted protein from Streptomyces coelicolor (172 aa),FASTA scores: opt: 214, E(): 3.4e-06, (42.55% identity in 94 aa overlap); Q38352|ORF360 from Lactococcus delbrueckii bacteriophage ll-H (360 aa), FASTA scores: opt: 211, E(): 9.5e-06, (40.0% identity in 115 aa overlap); P54334|XKDO_BACSU|XKDO phage-like element PBSX protein from Bacillus subtilis (1332 aa), FASTA scores: opt: 209, E(): 3.6e-05, (38.35% identity in 86 aa overlap); etc. Also similar to P71594|P71594|Rv0024|MTCY10H4.24 hypothetical 30.3 KDA protein from Mycobacterium tuberculosis (281 aa),FASTA scores: opt: 265, E(): 3.9e-09, (29.25% identity in 287 aa overlap).; hypothetical protein 4382851 886216 Rv3896c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218413.1 4381943 R 83332 CDS NP_218414.1 15611033 886225 complement(4383008..4383640) 1 NC_000962.3 Rv3897c, (MTCY15F10.15), len: 210 aa. Conserved hypothetical protein, highly similar in part to Q10691|YK83_MYCTU|Rv2083|MT2145|MTCY49.22 hypothetical 30.8 KDA protein from Mycobacterium tuberculosis (314 aa) FASTA scores: opt: 815, E(): 4.7e-26, (73.05% identity in 167 aa overlap). Similarity to MTCY49.22 suggests that this is a continuation of MTCY15F10.14. There is a frameshift mutation near 3'-end with respect to this sequence as well,similarity to MTCY49.22 continues in an overlapping ORF. Sequence appears to be correct.; hypothetical protein 4383640 886225 Rv3897c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218414.1 4383008 R 83332 CDS NP_218415.1 15611034 886233 complement(4383653..4383985) 1 NC_000962.3 Rv3898c, (MTCY15F10.14), len: 110 aa. Conserved hypothetical protein. Highly similar, but in part, to Q10691|YK83_MYCTU|Rv2083|MT2145|MTCY49.22 hypothetical 30.8 KDA protein from Mycobacterium tuberculosis (314 aa) FASTA scores: opt: 204, E(): 0.00042, (50.6% identity in 81 aa overlap). Similarity suggests it should be in frame with next ORF and that the stop codon could be read through, the sequence appears to be correct. Homology lost upstream at 15138 gatc sequence may suggest discontinuity due to chimerism in cY15F10 or cY49.; hypothetical protein 4383985 886233 Rv3898c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218415.1 4383653 R 83332 CDS NP_218416.1 15611035 886228 complement(4384147..4385379) 1 NC_000962.3 Rv3899c, (MTCY15F10.13), len: 410 aa. Conserved hypothetical protein, similar in part to proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551. Region between aa 29-80 is strictly identical to P96909 hypothetical 15.1 KDA protein (fragment) (143 aa) FASTA scores: opt: 562, E(): 4e-16, (69.0% identity in 142 aa overlap); and the N-terminal end is highly similar, but longer 65 aa, to O07266 hypothetical 13.7 KDA protein (fragment) (143 aa), FASTA scores: opt: 562, E(): 4e-16,(69.0% identity in 142 aa overlap). Highly similar to C-terminal end of Q10690|YK82_MYCTU|Rv2082|MTCY49.21 hypothetical 73.6 KDA protein (721 aa), FASTA scores: opt: 1388, E(): 1.5e-48, (55.25% identity in 409 aa overlap). And similar to P71599|Rv0029|MTCY10H4.29 hypothetical 39.6 KDA protein (365 aa), FASTA scores: opt: 403, E(): 1.7e-09,(33.75% identity in 252 aa overlap). Note that MTCY15F10.12 and MTCY15F10.13 appear frameshifted with respect to MTCY49.21 although the sequence appears to be correct.; hypothetical protein 4385379 886228 Rv3899c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218416.1 4384147 R 83332 CDS NP_218417.1 15611036 886235 complement(4385373..4386308) 1 NC_000962.3 Rv3900c, (MTCY15F10.12), len: 311 aa. Conserved hypothetical ala-rich protein, highly similar to N-terminal end of Q10690|YK82_MYCTU|Rv2082|MTCY49.21 hypothetical 73.6 KDA protein from Mycobacterium tuberculosis (721 aa), FASTA scores: opt: 592, E(): 2.7e-22, (37.15% identity in 280 aa overlap). Note that MTCY15F10.12 and MTCY15F10.13 appear frameshifted with respect to MTCY49.21 although the sequence appears to be correct. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Conserved hypothetical alanine rich protein 4386308 886235 Rv3900c Mycobacterium tuberculosis H37Rv Conserved hypothetical alanine rich protein NP_218417.1 4385373 R 83332 CDS NP_218418.1 15611037 886226 complement(4386365..4386814) 1 NC_000962.3 Rv3901c, (MTCY15F10.11), len: 149 aa. Possible membrane protein (hydrophobic stretch from ~30-52), showing some similarity with O53200|Rv2473|MTV008.29 hypothetical 25.1 KDA protein from Mycobacterium tuberculosis (238 aa),FASTA scores: opt: 147, E(): 0.036, (31.35% identity in 134 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).; Possible membrane protein 4386814 886226 Rv3901c Mycobacterium tuberculosis H37Rv Possible membrane protein NP_218418.1 4386365 R 83332 CDS NP_218419.1 15611038 886236 complement(4387365..4387895) 1 NC_000962.3 Rv3902c, (MTCY15F10.10), len: 176 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).; Hypothetical protein 4387895 886236 Rv3902c Mycobacterium tuberculosis H37Rv Hypothetical protein NP_218419.1 4387365 R 83332 CDS NP_218420.1 15611039 886229 complement(4387892..4390432) 1 NC_000962.3 Rv3903c, (MTCY15F10.08), len: 846 aa. Hypothetical unknown ala-, pro-rich protein.; Hypothetical alanine and proline rich protein 4390432 886229 Rv3903c Mycobacterium tuberculosis H37Rv Hypothetical alanine and proline rich protein NP_218420.1 4387892 R 83332 CDS NP_218421.1 15611040 886237 complement(4390437..4390709) 1 NC_000962.3 Rv3904c, (MT4023, MTCY15F10.07), len: 90 aa. EsxE,ESAT-6 like protein, hypothetical unknown ala-rich protein. Belongs to the ESAT6 family (see citation below).; Putative ESAT-6 like protein EsxE (hypothetical alanine rich protein) (ESAT-6 like protein 12) 4390709 esxE 886237 esxE Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxE (hypothetical alanine rich protein) (ESAT-6 like protein 12) NP_218421.1 4390437 R 83332 CDS NP_218422.1 15611041 886239 complement(4390720..4391031) 1 NC_000962.3 Rv3905c, (MT4024, MTCY15F10.06), len: 103 aa. EsxF,ESAT-6 like protein (see citation below), hypothetical unknown ala-, gly-rich protein, ESAT-6 like protein. Belongs to the ESAT6 family.; Putative ESAT-6 like protein EsxF (hypothetical alanine and glycine rich protein) (ESAT-6 like protein 13) 4391031 esxF 886239 esxF Mycobacterium tuberculosis H37Rv Putative ESAT-6 like protein EsxF (hypothetical alanine and glycine rich protein) (ESAT-6 like protein 13) NP_218422.1 4390720 R 83332 CDS NP_218423.1 15611042 886221 complement(4391097..4391606) 1 NC_000962.3 Rv3906c, (MTCY15F10.05), len: 169 aa. Conserved hypothetical protein, strongly related to Q50578|AT9S (sod related in Escherichia coli) from Mycobacterium tuberculosis strain aoyama B (155 aa), but apparently different as flanking sequences differ and shorter 43 aa,FASTA scores: opt: 548, E(): 1.3e-26, (79.4% identity in 102 aa overlap). Selfmarch results suggest that Rv3906c is not related to any other hypothetical protein from Mycobacterium tuberculosis strain H37Rv except itself. Shows also similarity with Q9VFR2|CG9297 hypothetical protein from Drosophila melanogaster (Fruit fly) (930 aa),FASTA scores: opt: 221, E(): 4.9e-06, (36.95% identity in 157 aa overlap); Q9HQ55|CBP|VNG1320G calcium-binding protein homology from Halobacterium sp. strain NRC-1 (385 aa) FASTA scores: opt: 143, E(): 0.13, (35.65% identity in 160 aa overlap); Q24795 calcium-binding protein (fragment) from Echinococcus granulosus (338 aa), FASTA scores: opt: 140, E(): 0.17, (33.95% identity in 156 aa overlap).; hypothetical protein 4391606 886221 Rv3906c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218423.1 4391097 R 83332 CDS YP_178026.1 57117168 886240 complement(4391631..4393073) 1 NC_000962.3 Rv3907c, (MTCY15F10.04), len: 480 aa. Probable pcnA,polynucleotide polymerase, equivalent to Q9CCY1|PCNA|ML2697 PCNA protein from Mycobacterium leprae (486 aa), FASTA scores: opt: 2713, E(): 4.3e-160, (84.1% identity in 478 aa overlap); and Q59534|PCNB POLYA polymerase from Mycobacterium leprae (411 aa) FASTA scores: opt: 2077, E(): 7.1e-121, (82.55% identity in 373 aa overlap). Also highly similar to many e.g. Q9X8T2|SCH24.18 putative RNA nucleotidyltransferase from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1856, E(): 3.7e-107, (61.55% identity in 455 aa overlap); Q9ZN65 POLYA polymerase from Prevotella ruminicola (Bacteroides ruminicola) (479 aa),FASTA scores: opt: 830, E(): 8.5e-44, (34.85% identity in 445 aa overlap); P42977|PAPS_BACSU poly(A) polymerase from Bacillus subtilis (397 aa), FASTA scores: opt: 479, E(): 3.5e-22, (29.35% identity in 450 aa overlap); etc. Contains: PS00017 ATP/GTP-binding site motif A (P-loop),PS00018 EF-hand calcium-binding domain, and probably less significant a PS00237 G-protein coupled receptor signature,and PS00639 Eukaryotic thiol (cysteine) proteases histidine active site. Belongs to the tRNA nucleotidyltransferase / poly(A) polymerase family.; Probable poly(A) polymerase PcnA (polynucleotide adenylyltransferase) (NTP polymerase) (RNA adenylating enzyme) (poly(A) polymerase) 4393073 pcnA 886240 pcnA Mycobacterium tuberculosis H37Rv Probable poly(A) polymerase PcnA (polynucleotide adenylyltransferase) (NTP polymerase) (RNA adenylating enzyme) (poly(A) polymerase) YP_178026.1 4391631 R 83332 CDS NP_218425.1 15611044 886242 4393449..4394195 1 NC_000962.3 Rv3908, (MTCY15F10.03c), len: 248 aa. Possible mutT4, mutator protein, equivalent to Q50195|ML2698|L222-ORF6 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1270,E(): 3.4e-62, (79.05% identity in 248 aa overlap). Also similar to O66548|APFA|AQ_158 hydrolase from Aquifex aeolicus (134 aa), FASTA scores: opt: 300, E(): 1.1e-09,(37.3% identity in 142 aa overlap); and similarity with other various proteins e.g. O93721 diadenosine 5'5'''-P1,P4-tetraphosphate pyrophosphohydrolase from Pyrobaculum aerophilum (143 aa), FASTA scores: opt: 205,E(): 0.00017, (34.85% identity in 109 aa overlap); Q9HS29|APA|VNG0431G diadenosine tetraphosphate pyrophosphohydrolase from Halobacterium sp. strain NRC-1 (142 aa), FASTA scores: opt: 199, E(): 0.00036, (34.0% identity in 147 aa overlap); Q9YA58|APE2080 hypothetical 19.2 KDA protein from Aeropyrum pernix (175 aa) FASTA scores: opt: 191, E(): 0.0012, (36.9% identity in 141 aa overlap); etc. Also similar to P95110|MUTT1|Rv2985|MTCY349.02 hypothetical 34.7 KDA protein from Mycobacterium tuberculosis (317 aa) FASTA scores: opt: 224, E(): 3e-05, (34.05% identity in 144 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). Seems to belong to the NUDIX hydrolase family.; Possible mutator protein MutT4 4394195 mutT4 886242 mutT4 Mycobacterium tuberculosis H37Rv Possible mutator protein MutT4 NP_218425.1 4393449 D 83332 CDS NP_218426.1 15611045 886245 4394192..4396600 1 NC_000962.3 Rv3909, (MTCY15F10.02c), len: 802 aa. Conserved protein, equivalent to Q9CCY0|ML2699 putative secreted protein from Mycobacterium leprae (797 aa) FASTA scores: opt: 3777, E(): 8.8e-206, (72.35% identity in 803 aa overlap). Note that the N-terminal end is highly similar to Q50196|L222-ORF7 (286 aa), FASTA scores: opt: 1213, E(): 2.7e-61, (71.75% identity in 255 aa overlap); and the C-terminal end is highly similar to Q50197|L222-ORF8 also from Mycobacterium leprae (512 aa) FASTA scores: opt: 2375,E(): 9.9e-127, (71.8% identity in 518 aa overlap). Shows some similarity with N-terminal end of Q9I2M3|PA1874 hypothetical protein from Pseudomonas aeruginosa (2468 aa),FASTA scores: opt: 171, E(): 0.13, (22.9% identity in 672 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008).; hypothetical protein 4396600 886245 Rv3909 Mycobacterium tuberculosis H37Rv hypothetical protein NP_218426.1 4394192 D 83332 CDS NP_218427.1 15611046 886247 4396597..4400151 1 NC_000962.3 Rv3910, (MTCY15F10.01c.MTV028.01), len: 1184 aa. Probable conserved transmembrane protein (hydrophobic domain ~50-550), equivalent to Q9CCX9|ML2700 possible conserved membrane protein from Mycobacterium leprae (1206 aa), FASTA scores: opt: 5554, E(): 0, (75.15% identity in 1182 aa overlap); and highly similar, but shorter 380 aa,to Q50199|L222-ORF10 from Mycobacterium leprae (784 aa) FASTA scores: opt: 3297, E(): 5.5e-170, (68.8% identity in 769 aa overlap); and at the N-terminal end with Q50198|L222-ORF also from Mycobacterium leprae (379 aa) FASTA scores: opt: 1955, E(): 5.7e-98, (88.4% identity in 353 aa overlap) (ORFs 9 and 10 are adjacent on L222). Also similar in part (principally at the N-terminal end) to other membrane proteins e.g. Q9X8T0|SCH24.16c putative transmembrane protein from Streptomyces coelicolor (811 aa), FASTA scores: opt: 573, E(): 2.8e-23, (31.05% identity in 573 aa overlap); O05467|MVIN_RHITR integral membrane protein virulence factor MVIN homolog from Rhizobium tropici (533 aa), FASTA scores: opt: 468, E(): 9e-18,(27.1% identity in 524 aa overlap); P56882|MVIN_RHIME integral membrane protein virulence factor MVIN homolog from Rhizobium meliloti (Sinorhizobium meliloti) (535 aa),FASTA scores: opt: 453, E(): 5.8e-17, (26.2% identity in 557 aa overlap); etc.; Probable conserved transmembrane protein 4400151 886247 Rv3910 Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218427.1 4396597 D 83332 CDS NP_218428.1 15611047 886246 4400186..4400854 1 NC_000962.3 Rv3911, (MTV028.02), len: 222 aa. Possible sigM,alternative RNA polymerase sigma factor (see Gomez et al.,1997; Chen et al., 2000), highly similar to others e.g. Q9S6U3|SCH24.14c (alias O86856|SIGT) putative RNA polymerase sigma factor from Streptomyces coelicolor (236 aa), FASTA scores: opt: 336, E(): 2.8e-13, (41.5% identity in 212 aa overlap); Q98KG8|MLR1481 probable RNA polymerase sigma subunit from Rhizobium loti (Mesorhizobium loti) (307 aa), FASTA scores: opt: 221, E(): 2.9e-06, (32.95% identity in 179 aa overlap); Q9A4S9|CC2751 putative RNA polymerase sigma factor from Caulobacter crescentus (186 aa), FASTA scores: opt: 217, E(): 3.3e-06, (36.95% identity in 138 aa overlap); etc. Also similarity with other mycobacterial factors e.g. O06289|SIGE|Rv1221|MTCI61.04 putative RNA polymerase sigma factor from Mycobacterium tuberculosis (257 aa), FASTA scores: opt: 193, E(): 0.00012, (33.15% identity in 163 aa overlap); and O05735|SIGE putative RNA polymerase sigma factor from Mycobacterium avium (251 aa),FASTA scores: opt: 192, E(): 0.00014, (33.15% identity in 163 aa overlap). Equivalent to AAK48395|MT4030 RNA polymerase sigma-70 factor from Mycobacterium tuberculosis strain CDC1551 (196 aa) but without similarity at the C-terminal end. Belongs to the sigma-70 factor family, ECF subfamily.; Possible alternative RNA polymerase sigma factor SigM 4400854 sigM 886246 sigM Mycobacterium tuberculosis H37Rv Possible alternative RNA polymerase sigma factor SigM NP_218428.1 4400186 D 83332 CDS NP_218429.1 15611048 886234 4400870..4401634 1 NC_000962.3 Rv3912, (MTV008.03), len: 254 aa. Hypothetical unknown ala-rich protein. Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010).; Hypothetical alanine rich protein 4401634 886234 Rv3912 Mycobacterium tuberculosis H37Rv Hypothetical alanine rich protein NP_218429.1 4400870 D 83332 CDS NP_218430.1 15611049 886232 4401728..4402735 1 NC_000962.3 Rv3913, (MT4032, MTV028.04), len: 335 aa. Probable trxB2, thioredoxin reductase (see citation below),equivalent to O30973|TRXB_MYCSM thioredoxin reductase from Mycobacterium smegmatis (311 aa), FASTA scores: opt: 1575,E(): 1.8e-87, (78.35% identity in 305 aa overlap); and highly similar, but shorter at C-terminus, to P46843|TRXB_MYCLE|TRXB/a|TRX|ML2703 bifunctional thioredoxin reductase/thioredoxin from Mycobacterium leprae (458 aa), FASTA scores: opt: 1766, E(): 8.7e-99, (83.25% identity in 328 aa overlap). Also highly similar to many e.g. P52215|TRXB_STRCO|SCH24.12 from Streptomyces coelicolor (321 aa), FASTA scores: opt: 1249, E(): 7.2e-68,(60.4% identity in 313 aa overlap); Q9Z8M4|TRXB_CHLPN from Chlamydia pneumoniae (Chlamydophila pneumoniae) (311 aa),FASTA scores: opt: 978, E(): 1.3e-51, (49.85% identity in 307 aa overlap); P09625|TRXB_ECOLI|B0888 from Escherichia coli strain K12 (320 aa), FASTA scores: opt: 948, E(): 8.6e-50, (49.2% identity in 309 aa overlap); etc. Contains PS00573 Pyridine nucleotide-disulphide oxidoreductases class-II active site. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-II. Cofactor: FAD (by similarity).; Probable thioredoxin reductase TrxB2 (TRXR) (TR) 4402735 trxB2 886232 trxB2 Mycobacterium tuberculosis H37Rv Probable thioredoxin reductase TrxB2 (TRXR) (TR) NP_218430.1 4401728 D 83332 CDS NP_218431.1 15611050 886241 4402732..4403082 1 NC_000962.3 Rv3914, (MT4033, MTV028.05), len: 116 aa. TrxC (alternate gene names: mpt46, trx, trxA *), thioredoxin (see citations below), equivalent to O30974|THIO_MYCSM|TRXA thioredoxin from Mycobacterium smegmatis (112 aa), FASTA scores: opt: 576, E(): 2.1e-32, (80.2% identity in 111 aa overlap); and also equivalent to C-terminal end of P46843|TRXB_MYCLE|TRXB/a|TRX|ML2703 bifunctional thioredoxin reductase/thioredoxin from Mycobacterium leprae (458 aa), FASTA scores: opt: 628, E(): E(): 2e-35, (82.9% identity in 117 aa overlap). Also highly similar to many e.g. P80579|THIO_ALIAC from Alicyclobacillus acidocaldarius (Bacillus acidocaldarius) (105 aa), FASTA scores: opt: 411,E(): 3e-21, (57.15% identity in 105 aa overlap); P00275|THI1_CORNE from Corynebacterium nephridii (105 aa),FASTA scores: opt: 394, E(): 4.3e-20, (56.7% identity in 97 aa overlap); P00274|THIO_ECOLI|TRXA|TSNC|FIPA|B3781 from Escherichia coli and Salmonella typhimurium strain K12 and LT2 respectively (108 aa), FASTA scores: opt: 364, E(): 4.7e-18, (54.45% identity in 101 aa overlap); etc. Also similar to O53162|TRXB|Rv1471|MTV007.18 thioredoxin from Mycobacterium tuberculosis (123 aa), FASTA scores: E(): 2.3e-15, (41.9% identity in 93 aa overlap). Contains PS00194 Thioredoxin family active site. Belongs to the thioredoxin family. The product of this CDS is supposedly secreted. In this case, this protein could exert its free radical scavenging activity inside macrophages. (*) Warning: note that Rv1470|MTV007.17 correspond also to trxA.; Thioredoxin TrxC (TRX) (MPT46) 4403082 trxC 886241 trxC Mycobacterium tuberculosis H37Rv Thioredoxin TrxC (TRX) (MPT46) NP_218431.1 4402732 D 83332 CDS YP_178027.1 57117169 886250 4403192..4404412 1 NC_000962.3 Rv3915, (MTV028.06), len: 406 aa. Probable peptidoglycan hydrolase, equivalent to Q9CCX8|ML2704 putative hydrolase from Mycobacterium leprae (406 aa) FASTA scores: opt: 2341, E(): 2.7e-138, (86.95% identity in 406 aa overlap); the N-terminal end is highly similar to Q59535 N-acetymuramyl-L-alanine amidase from Mycobacterium leprae (205 aa), FASTA scores: opt: 1046, E(): 5.7e-58, (84.85% identity in 185 aa overlap). Also similar to other hydrolases (especially amidases) e.g. C-terminal end of Q9K6R3|LYTC|BH3665 N-acetylmuramoyl-L-alanine amidase (major autolysin) from Bacillus halodurans (588 aa), FASTA scores: opt: 363, E(): 4.3e-15, (33.15% identity in 356 aa overlap); Q9PKC7|TC0539 putative N-acetylmuramoyl-L-alanine amidase from Chlamydia muridarum (268 aa), FASTA scores: opt: 285, E(): 1.6e-10, (26.05% identity in 242 aa overlap) (RV3915 product appears longer 127 aa); Q9S596|PDCA penicillin-resistant DD-carboxypeptidase from Myxococcus xanthus (302 aa), FASTA scores: opt: 270, E(): 1.5e-09,(39.85% identity in 158 aa overlap); etc. Note that previously known as cwlM. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).; Probable peptidoglycan hydrolase 4404412 886250 Rv3915 Mycobacterium tuberculosis H37Rv Probable peptidoglycan hydrolase YP_178027.1 4403192 D 83332 CDS NP_218433.1 15611052 886249 complement(4404433..4405167) 1 NC_000962.3 Rv3916c, (MTV028.07c), len: 244 aa. Conserved hypothetical protein, equivalent to Q50200|ML2705|L222-ORF1 hypothetical protein from Mycobacterium leprae (259 aa),FASTA scores: opt: 1266, E(): 2e-74, (76.4% identity in 250 aa overlap). Also highly similar (but with gaps) to Q9R3S2|STH24.10 hypothetical 22.6 KDA protein from Streptomyces coelicolor (205 aa), FASTA scores: opt: 387,E(): 7.5e-18, (40.25% identity in 231 aa overlap). Predicted to be an outer membrane protein (See Song et al.,2008).; hypothetical protein 4405167 886249 Rv3916c Mycobacterium tuberculosis H37Rv hypothetical protein NP_218433.1 4404433 R 83332 CDS NP_218435.2 57117170 886244 complement(4405457..4406491) 1 NC_000962.3 Rv3917c, (MTV028.08c, MT4036), len: 344 aa. Probable parB, chromosome partitioning protein, equivalent to Q50201|PARB_MYCLE|ML2706 probable chromosome partitioning protein from Mycobacterium leprae (333 aa), FASTA scores: opt: 1654, E(): 1.6e-88, (78.6% identity in 332 aa overlap). Also highly similar to to others e.g. Q9S6U1|STH24.09 putative partitioning or sporulation protein from Streptomyces coelicolor (328 aa), FASTA scores: opt: 966, E(): 9.7e-49, (58.55% identity in 287 aa overlap) (no similarity on N-terminus); Q9PB63|PARB_XYLFA|XF2281 probable chromosome partitioning protein from Xylella fastidiosa (310 aa), FASTA scores: opt: 598, E(): 1.8e-27, (38.65% identity in 326 aa overlap); P31857|PARB_PSEPU probable chromosome partitioning protein from Pseudomonas putida (290 aa),FASTA scores: opt: 573, E(): 4.6e-26, (40.35% identity in 322 aa overlap); etc. Contains probable helix-turn-helix motif at aa 179 to 200 (Score 1150, +3.1 0 SD). Belongs to the ParB family. Note that previously known as parA.; Probable chromosome partitioning protein ParB 4406491 parB 886244 parB Mycobacterium tuberculosis H37Rv Probable chromosome partitioning protein ParB NP_218435.2 4405457 R 83332 CDS NP_218434.2 57117171 886224 complement(4406488..4407531) 1 NC_000962.3 Rv3918c, (MTV028.09c), len: 347 aa. Probable parA,chromosome partitioning protein, highly similar to Q9CCX7|para|ML2707 putative cell division protein from Mycobacterium leprae (351 aa), FASTA scores: opt: 1679,E(): 2.9e-93, (78.1% identity in 347 aa overlap). Also highly similar to others e.g. Q9RFM1|para para protein from Streptomyces coelicolor (357 aa), FASTA scores: opt: 1197,E(): 2e-64, (60.45% identity in 306 aa overlap); Q98DZ3|MLL4479|para chromosome partitioning protein from Rhizobium loti (Mesorhizobium loti) (266 aa), FASTA scores: opt: 835, E(): 7.2e-43, (50.95% identity in 257 aa overlap); O05189|PARA_CAUCR chromosome partitioning protein from Caulobacter crescentus (267 aa), FASTA scores: opt: 813, E(): 1.5e-41, (51.35% identity in 261 aa overlap) (has its N-terminus shorter); etc. Equivalent to AAK48403 from Mycobacterium tuberculosis strain CDC1551 (381 aa) but shorter 34 aa. Also similar to other Mycobacterium tuberculosis proteins: MTCI125.30, FASTA scores: E(): 4.3e-32, (35.2% identity in 327 aa overlap); and MTCY07D11.13, FASTA scores: E(): 3e-30, (39.9% identity in 263 aa overlap). Belongs to the para family. Possible alternative start site at aa 107. Note that previously known as parB.; Probable chromosome partitioning protein ParA 4407531 parA 886224 parA Mycobacterium tuberculosis H37Rv Probable chromosome partitioning protein ParA NP_218434.2 4406488 R 83332 CDS NP_218436.2 448824821 886243 complement(4407528..4408202) 1 NC_000962.3 Rv3919c, (MT4038, MTV028.10c), len: 224 aa. Probable gid (alternate gene name: gidB), glucose-inhibited division protein B, equivalent, but shorter 20 aa, to Q9L7M3 putative GIDB (fragment) from Mycobacterium paratuberculosis (245 aa), FASTA scores: opt: 1018, E(): 4.8e-57, (73.95% identity in 211 aa overlap); and Q50203|GIDB_MYCLE|ML2708 glucose inhibited division protein B from Mycobacterium leprae (245 aa), FASTA scores: opt: 966, E(): 9.1e-54, (68.4% identity in 212 aa overlap). Also highly similar to many e.g. O54571|GIDB_STRCO|STH24.07 from Streptomyces coelicolor (239 aa), FASTA scores: opt: 654,E(): 3.9e-34, (47.95% identity in 221 aa overlap); Q9KNG5|VC2774 from Vibrio cholerae (210 aa), FASTA scores: opt: 300, E(): 6.9e-12, (38.15% identity in 139 aa overlap); P17113|GIDB_ECOLI|B3740|Z5240|ECS4682 from Escherichia coli (several strains) (207 aa), FASTA scores: opt: 287, E(): 4.5e-11, (34.8% identity in 138 aa overlap); etc. Contains PS00539 Pyrokinins signature. Belongs to the GIDB family. Nucleotide position 4407904 in the genome sequence has been corrected, G:A resulting in S100F.; Probable glucose-inhibited division protein B Gid 4408202 gid 886243 gid Mycobacterium tuberculosis H37Rv Probable glucose-inhibited division protein B Gid NP_218436.2 4407528 R 83332 CDS NP_218437.1 15611056 886255 complement(4408334..4408897) 1 NC_000962.3 Rv3920c, (MTV028.11c), len: 187 aa. Conserved protein, similar to jag protein, equivalent to Q9L7M2 hypothetical 20.1 KDA protein from Mycobacterium paratuberculosis (183 aa), FASTA scores: opt: 1004, E(): 7.3e-52, (85.05% identity in 187 aa overlap); and Q50204|ML2709 hypothetical protein similar to jag protein SPOIIIJ associated protein in bacillus subtilis from Mycobacterium leprae (193 aa), FASTA scores: opt: 871, E(): 4.4e-44, (73.05% identity in 193 aa overlap). Also similar to other bacterial proteins e.g. O54595|STH24.06|jag jag-like protein from Streptomyces coelicolor (170 aa),FASTA scores: opt: 593, E(): 6.7e-28, (62.85% identity in 167 aa overlap); Q9RCA6|jag|BH4063 jag protein homolog from Bacillus halodurans (207 aa), FASTA scores: opt: 282, E(): 1.1e-09, (35.0% identity in 140 aa overlap); Q9X1H1|TM1460 putative jag protein, putative from Thermotoga maritima (221 aa), FASTA scores: opt: 258, E(): 3e-08, (31.9% identity in 138 aa overlap);Q01620|JAG_BACSU jag protein (SPOIIIJ associated protein) from Bacillus subtilis (208 aa), FASTA scores: opt: 196, E(): 0.00012, (28.05% identity in 139 aa overlap); etc.; Conserved protein similar to jag protein 4408897 886255 Rv3920c Mycobacterium tuberculosis H37Rv Conserved protein similar to jag protein NP_218437.1 4408334 R 83332 CDS NP_218438.1 15611057 886238 complement(4408969..4410069) 1 NC_000962.3 Rv3921c, (MTV028.12c), len: 366 aa. Probable conserved transmembrane protein, equivalent to Q9L7M1 hypothetical 39.2 KDA protein from Mycobacterium paratuberculosis (353 aa), FASTA scores: opt: 2001, E(): 8.4e-100, (83.05% identity in 366 aa overlap); Q9CCX6|ML2710 putative conserved membrane protein from Mycobacterium leprae (380 aa), FASTA scores: opt: 1929,E(): 6.2e-96, (77.1% identity in 380 aa overlap); Q50205 CDS 27 on L222 from Mycobacterium leprae (312 aa) FASTA scores: opt: 1770, E(): 1.6e-87, (88.2% identity in 288 aa overlap). Also similar to other e.g. O54569|STH24.05 inner membrane protein. from Streptomyces coelicolor (431 aa),FASTA scores: opt: 412, E(): 6.5e-15, (33.45% identity in 266 aa overlap); O84253|CT251 60 KDA inner membrane protein from Chlamydia trachomatis (787 aa), FASTA scores: opt: 304, E(): 6e-09, (27.9% identity in 269 aa overlap); P29431|60IM_BUCAP 60 KDA inner-membrane protein homolog from Buchnera aphidicola (subsp. Schizaphis graminum) (536 aa), FASTA scores: opt: 282, E(): 6.7e-08, (36.1% identity in 108 aa overlap); etc.; Probable conserved transmembrane protein 4410069 886238 Rv3921c Mycobacterium tuberculosis H37Rv Probable conserved transmembrane protein NP_218438.1 4408969 R 83332 CDS NP_218439.1 15611058 886256 complement(4410053..4410415) 1 NC_000962.3 Rv3922c, (MTV028.13c), len: 120 aa. Possible hemolysin, highly similar to Q9L7M0|YIDD_MYCPA hypothetical 12.4 KDA protein from Mycobacterium paratuberculosis (115 aa), FASTA scores: opt: 521, E(): 1.9e-29, (65.2% identity in 112 aa overlap). Also highly similar to Q44066|HLYA_AERHY putative alpha-hemolysin from Aeromonas hydrophila (85 aa), FASTA scores: opt: 276, E(): 1.5e-12,(51.45% identity in 70 aa overlap); and to many bacterial hypothetical proteins from bacterium e.g. P22847|YIDD_ECOLI|B3704.1 hypothetical protein from Escherichia coli strain K12 (85 aa), FASTA scores: opt: 276, E(): 1.5e-12, (51.45% identity in 70 aa overlap).; Possible hemolysin 4410415 886256 Rv3922c Mycobacterium tuberculosis H37Rv Possible hemolysin NP_218439.1 4410053 R 83332 CDS NP_218440.3 448824822 886248 complement(4410412..4410789) 1 NC_000962.3 Rv3923c, (MT4041, MTV028.14c), len: 125 aa. RnpA,ribonuclease P protein component (see citations below),equivalent, but longer ~10 aa, to P46610|RNPA_MYCLE|ML2712 ribonuclease P protein component from Mycobacterium leprae (120 aa), FASTA scores: opt: 456, E(): 3.3e-24, (63.0% identity in 119 aa overlap); and Q9L7L9|RNPA from Mycobacterium paratuberculosis (119 aa), FASTA scores: opt: 426, E(): 3.5e-22, (60.65% identity in 122 aa overlap). Also similar to many e.g. P25817|RNPA_STRBI from Streptomyces bikiniensis (123 aa), FASTA scores: opt: 174,E(): 4.2e-05, (36.8% identity in 125 aa overlap); P25814|RNPA_BACSU from Bacillus subtilis (116 aa) FASTA scores: opt: 168, E(): 0.0001, (26.85% identity in 108 aa overlap); P48206|RNPA_STRCO|STH24.03 from Streptomyces coelicolor (123 aa), FASTA scores: opt: 166, E(): 0.00015,(37.6% identity in 125 aa overlap); etc. Contains PS00648 Bacterial Ribonuclease P protein component signature. Belongs to the RnpA family.; Ribonuclease P protein component RnpA (RNaseP protein) (RNase P protein) (protein C5) 4410789 rnpA 886248 rnpA Mycobacterium tuberculosis H37Rv Ribonuclease P protein component RnpA (RNaseP protein) (RNase P protein) (protein C5) NP_218440.3 4410412 R 83332 CDS NP_218441.1 15611060 886258 complement(4410786..4410929) 1 NC_000962.3 Rv3924c, (MTV028.15), len: 47 aa. rpmH, 50s ribosomal protein l34 (see citations below), equivalent to many mycobacterial 50S ribosomal protein L34 e.g. P46386|RL34_MYCLE|RPMH|ML2713 from Mycobacterium leprae (47 aa), FASTA scores: opt: 287, E(): 8.5e-17, (91.5% identity in 47 aa overlap); and Q9L7L8|RL34_MYCPA|RPMH from Mycobacterium paratuberculosis (47 aa), FASTA scores: opt: 281, E(): 2.6e-16, (89.35% identity in 47 aa overlap). Also highly similar to other ribosomal proteins e.g. P27901|RL34_STRCO|RPMH|STH24.02 from Streptomyces coelicolor (45 aa), FASTA scores: opt: 234, E(): 1.4e-12,(79.05% identity in 43 aa overlap); and P05647|RL34_BACSU|RPMH from Bacillus subtilis (44 aa) FASTA scores: opt: 229, E(): 3.7e-12, (72.35% identity in 47 aa overlap); etc. Contains PS00784 Ribosomal protein L34 signature. Belongs to the L34P family of ribosomal proteins.; 50S ribosomal protein L34 RpmH 4410929 rpmH 886258 rpmH Mycobacterium tuberculosis H37Rv 50S ribosomal protein L34 RpmH NP_218441.1 4410786 R 83332 CDS