-- dump date   	20240506_002517
-- class       	Genbank::Contig
-- table       	contig_comment
-- id	comment
NC_020419.1	REFSEQ INFORMATION: The reference sequence is identical toREFSEQ INFORMATION: The reference sequence is identical to AP009510.1.REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1.REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera;REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gutREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of theREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia;REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using aREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbiontsREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotypeREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, theREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cellsREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. TheseREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genomeREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16SREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence wasREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system.REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome AnnotationREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here:REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START##REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeqREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic GenomeREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP)REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference proteinREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNAREFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END##REFSEQ INFORMATION: The reference sequence is identical to AP009510.1. On Feb 28, 2013 this sequence version replaced NS_000191.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END## COMPLETENESS: full length.
NC_020420.1	REFSEQ INFORMATION: The reference sequence is identical toREFSEQ INFORMATION: The reference sequence is identical to AP009511.1.REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1.REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera;REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gutREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of theREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia;REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using aREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbiontsREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotypeREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, theREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cellsREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. TheseREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genomeREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16SREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence wasREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system.REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome AnnotationREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here:REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START##REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeqREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic GenomeREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP)REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference proteinREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNAREFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END##REFSEQ INFORMATION: The reference sequence is identical to AP009511.1. On Feb 28, 2013 this sequence version replaced NS_000192.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END## COMPLETENESS: full length.
NC_020421.1	REFSEQ INFORMATION: The reference sequence is identical toREFSEQ INFORMATION: The reference sequence is identical to AP009512.1.REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1.REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera;REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gutREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of theREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia;REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using aREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbiontsREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotypeREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, theREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cellsREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. TheseREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genomeREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16SREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence wasREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system.REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome AnnotationREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here:REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START##REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeqREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic GenomeREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP)REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference proteinREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNAREFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END##REFSEQ INFORMATION: The reference sequence is identical to AP009512.1. On Feb 28, 2013 this sequence version replaced NS_000193.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END## COMPLETENESS: full length.
NC_020422.1	REFSEQ INFORMATION: The reference sequence is identical toREFSEQ INFORMATION: The reference sequence is identical to AP009513.1.REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1.REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera;REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gutREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of theREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia;REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using aREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbiontsREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotypeREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, theREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cellsREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. TheseREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genomeREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16SREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence wasREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system.REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome AnnotationREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here:REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START##REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeqREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic GenomeREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP)REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference proteinREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNAREFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END##REFSEQ INFORMATION: The reference sequence is identical to AP009513.1. On Feb 28, 2013 this sequence version replaced NS_000194.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider               :: NCBI RefSeq Annotation Date                   :: 02/17/2024 01:35:20 Annotation Pipeline               :: NCBI Prokaryotic Genome                                      Annotation Pipeline (PGAP) Annotation Method                 :: Best-placed reference protein                                      set; GeneMarkS-2+ Annotation Software revision      :: 6.6 Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total)                     :: 1,200 CDSs (total)                      :: 1,150 Genes (coding)                    :: 1,139 CDSs (with protein)               :: 1,139 Genes (RNA)                       :: 50 rRNAs                             :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S) tRNAs                             :: 45 ncRNAs                            :: 2 Pseudo Genes (total)              :: 11 CDSs (without protein)            :: 11 Pseudo Genes (ambiguous residues) :: 0 of 11 Pseudo Genes (frameshifted)       :: 1 of 11 Pseudo Genes (incomplete)         :: 6 of 11 Pseudo Genes (internal stop)      :: 4 of 11 CRISPR Arrays                     :: 2 ##Genome-Annotation-Data-END## COMPLETENESS: full length.